LOCUS       DBMDQJ010000001        11530 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_2643_length_11530_cov_1.508706, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000001 DBMDQJ010000000
VERSION     DBMDQJ010000001.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 11530)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11530)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11530
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2643_length_11530_cov_1.508706"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="ACZO9R_00005"
     CDS             complement(<1..580)
                     /locus_tag="ACZO9R_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HIR0911485.1"
                     /translation="MREKIFNIIQIGDKSNRVSRAFDIFITITIASNILVTFLQTFEE
                     LDFLSGFFFAVEVITLLIFCVEYVLRIWTADLLYPGMRKCRARLRFMVSFDGVVDILT
                     IVPVFFLSGFVIFRMLRVARIFHLFRLNARYDSFNVITTVLYEKRNQIISSVFIVLIL
                     MLASSLCMYSVEHDSQPEVFRNAFSGIWWSMST"
     gene            complement(634..1542)
                     /locus_tag="ACZO9R_00010"
     CDS             complement(634..1542)
                     /locus_tag="ACZO9R_00010"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HIR0911486.1"
                     /translation="MNLGIDLCGVALKNPVMTASGTFGSGEEYSEFVDLNKLGAVVTK
                     GVANVPWEGNPTPRVAEVYGGMLNAIGLQNPGIDLFIDRDIPFLKKYDTKIIVNVCGH
                     TTEEYIDVVERLSDQPVDLLEINISCPNVKEGGIAFGQNPKMVEEVTKEMKRHAKQPV
                     IMKLSPNVTDITEMARAAEAGGADALSMINTITGMKIDIHRRCFALANRTGGMSGPAV
                     HPVAVRMVYQSAQAVKIPIIGMGGIQTAEDAIEMILAGATAVSVGTANFYHPHATEEI
                     VDGIRAYMERYQVEDIRELIGAVHDR"
     gene            complement(1542..2318)
                     /locus_tag="ACZO9R_00015"
     CDS             complement(1542..2318)
                     /locus_tag="ACZO9R_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HIR0911487.1"
                     /translation="MHTTQQKFEETAIIIKQEEIADDIYSMWLHTEQIAAHAKAGQFV
                     SVYCKEGSRLLPRPISICEIDRKDGALRLVYRVAGKGTEEFSSMHTGMQLKVIGPLGN
                     GFPKQEKKAFLIGGGIGIPPMLELAKELNCGKQIVLGYRDELFLLDEFRKQGEVYIAT
                     EDGSVGTEGNVLDAIRENGLTADVIYACGPLPMLRALKEYAAKEDIECWISMEERMAC
                     GIGACLACVCKSTEKDAHSNVKNKRVCKEGPVFRAEEVDF"
     gene            complement(2325..3656)
                     /locus_tag="ACZO9R_00020"
     CDS             complement(2325..3656)
                     /locus_tag="ACZO9R_00020"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR0911488.1"
                     /translation="MTILIKNGRVINPATGTDETLDVLVENGVVARTEKSIKEKCDRV
                     IDAKGCFVMPGFIDMHVHLRDPGFEQKETIETGARAAVHGGFTTILAMPNTKPVVDNP
                     DVVNYVHNKAKTVGAANVLQVGAVTKGQQGKELSDIEGMVKAGIPAISEDGKSVMNAQ
                     LYREAMELAAKYNIAVLAHCEDINLVNGGVMNEDVNARELGLGGITNSVEDIIIARDI
                     MLSRDTGARLHLCHCSTKDSASMVKHAKMEGIHVTAEVCPHHFTLTSDDIRKIEPTVD
                     TEKKVAIEADADTNYKMNPPLRTKEDVQALKEGLRDDVMDVIATDHAPHTFEDKNTSM
                     KSAPFGIVGLETAACLTYTELVLGGYLTPMQMAEKMSYNPAKIIGIDKGDIEPGKVAD
                     IVIFDPDETYVIDKNTFFSKGRNTPFDGRKVTGKVRMTLVNGQVVYEDKEK"
     gene            complement(3699..4943)
                     /gene="hflX"
                     /locus_tag="ACZO9R_00025"
     CDS             complement(3699..4943)
                     /gene="hflX"
                     /locus_tag="ACZO9R_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HIR0911489.1"
                     /translation="MAEIIELEDQIEKVILVGVSEQDGDDAEDSVAELAELVRTAGAV
                     VVGTLIQKRELMHPGTYVGTGKVAEIAAMIAERGATGIVCDDELSPAQLKNLEQMLDT
                     KVMDRTLIILDIFAARATTSEGKIQVELAQLKYRLSRLTGLGRSMSRLGGGIGTRGPG
                     EKKLEMDRRLIKDRIAQLNRELKEVRQHREITRAKRTRNGMPVAAIVGYTNAGKSTLL
                     NRLTDAGVLEEDKLFATLDPTTRVLELPGRQEILLTDTVGFIRKLPHHLIEAFKSTLE
                     EAKYADYILHVVDASNPQHEKQMHIVYDTLYQLDIREKTVITLFNKQDAVTEREPLHD
                     LRADHTLAVSAREGTGLDELKELLAELLRENKVLIERTIAYADAGILQQIRKQGELLA
                     EDYRPEGIYVKAYVPLELYGKL"
     gene            complement(4956..6101)
                     /locus_tag="ACZO9R_00030"
     CDS             complement(4956..6101)
                     /locus_tag="ACZO9R_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0911490.1"
                     /translation="MRRRMEINHHRTYRGKAAVFLLVTAALLTGCASERTEDELSYRK
                     IGIESMQSGDYEGALAAFDGALACCTGKITDLEIDICYYKAAAQYAAQDTEGALGTYN
                     AMIDYDGKNANAYYLRGCLKLGTGDTDGAKQDFADAVKYNHSDYELYINIYKNLAAYD
                     LAEDGTAYLNDAFSIKGNDAESLAYRGEIYYLLGQYDNAIKELTSAIDAGSAEANLTL
                     AQVYEAQQDTANAQTYYQAYVDAGVADSEAMNALAQIEMGKLNYSGALSYIEQGLSME
                     SVPNRRALLQNQIICMEYTGDFAGAYEVMTAYTAAYPDDAEALREYTFLKSRVDTPAG
                     TEVQGEVVVTEDIGTAEADSGTDGTDGADGTDSGTDAGTDGSGDSGQ"
     gene            complement(6216..7208)
                     /locus_tag="ACZO9R_00035"
     CDS             complement(6216..7208)
                     /locus_tag="ACZO9R_00035"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR0911491.1"
                     /translation="MMRYIDMHCDTLGSALAQKTETFLELDRTMVDGLRLQQAEAGAQ
                     FFAMFLPQRNDPDWFGLEEMPSAEVLMRKMYDIFCHTMEQYGDRFAAAHNAEELKQNQ
                     NEGKISAFLTIENGALIDGKMDKIREIYDLGVRLVTLTWNDDNCLGHCHSKKPEEMSR
                     GLTGFGKEAVSYMQELGILVDVSHLSDGGFRDVAETSRRSGVPFVASHSNCRALAPAT
                     RNLTDAMIRELAECGGVAGLNLEPTFLNEDETDKVSRIERICDHAMHLIDRGGIECVG
                     LGTDFDGISGKFEISDCTKLPLLFDALRKRGLSEDAVEQIAYKNTARVIRDGMR"
     gene            complement(7444..9696)
                     /locus_tag="ACZO9R_00040"
     CDS             complement(7444..9696)
                     /locus_tag="ACZO9R_00040"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR0911492.1"
                     /translation="MNVQEIVNNRYGKGIAQCSNEEIYYALLEMTKSMAKEKESNEGK
                     RKLYYISAEFLIGKLLSNNLINLGIYEEVKQVLAENGKNLAEIEEVEPEPSLGNGGLG
                     RLAACFLDSIATLGLNGDGVGLNYHYGLFKQVFEHNLQHEEPNPWIEKESWLTKTNVT
                     YPVQFGGFTLQSRLYDIDVIGYENRTTKLHLFDVETVDESLVGDGINFDKEDIAKNLT
                     LFLYPDDSDDKGRILRVYQQYFMVSNAAQLIIDETLARGGDLHKLNEYAAIQINDTHP
                     SMVIPELIRLLMQRGILMDEAIEIVSKTCAYTNHTILAEALEKWPIHFLEKAVPQLLP
                     IIYELNSRVVRKYDDKSVAIIDDEKRVHMAHMDIHYGYSVNGVAYLHTEILKNTELNN
                     FYKIYPEKFNNKTNGITFRRWLLHCNHELADLIESLIGPGFKKDAMELEKLGALVDDA
                     SVLQRLLDVKNSRKTELKDYLAKTQGIVLDDNSIYDIQIKRLHEYKRQQMNALYVIHK
                     YFEIKAGKKPTRPITVIFGAKAAPAYVIAKDIIHLILCLSELIANDPEVSPYLKVVMV
                     ENYNVTLAEKLIPAADIHEQISLASKEASGTSNMKFMLNGAVAIGTMDGANVEMHQFV
                     GDDNIYIFGESSEEVIKHYEKADYVSRSYYENDANIKRAVDFIVSDEMMAVGCRENLE
                     RLYNELLNKDWFMTLPDFKDYVATKERIYADYEDRMAWAKKMLINISKAGFFSSDRTI
                     AQYNEDIWHL"
     gene            9871..10758
                     /locus_tag="ACZO9R_00045"
     CDS             9871..10758
                     /locus_tag="ACZO9R_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR0911493.1"
                     /translation="MNILEYENYQEKIPHGNPLFPYITYPCSIPLDFSRVPLHWHDEM
                     EIIYIKKGTGAITVDFKSYVVTEGTIALILPGRLHSIEQYMQESMEYENIIFHPGILI
                     SKKTDTSNTEFLAPLLSGVLSVPLLYQPGSPCYAEIAACIDANDEISRTNPPGYQFFI
                     KSQLFMLFYILFSKCLLKEPAKRDHKSLEKMKLILKYLENHYMERITIADISAEVGLS
                     QSHFMKYFKNTMGTSFIEYLNEYRLTMASRLLISSESSILAIAEEVGYENLSYFNRTF
                     KKRFGMTPRDYRKQSTKQL"
     gene            complement(11006..>11530)
                     /locus_tag="ACZO9R_00050"
     CDS             complement(11006..>11530)
                     /locus_tag="ACZO9R_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024743.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR0911494.1"
                     /translation="RMDIDARIKLKSVKNNRNIHFDTMDFDIGAADADFASGSVNCRT
                     MELNVGAGELTGADFAVTGTLDIKLGAGDIDLEGGTYKDIKLDCGMGSFALDGIVAEN
                     VKAHCGMGDGSITLRGREEDYNYKMSCGMGDLTVNGESYSDLSGSYKVTNAGAVGTID
                     LDCGMGSIDFDIES"
BASE COUNT         2511 a         3388 c         2484 g         3147 t
ORIGIN      
        1 gcgttgacat gctccaccag atgccgctga aggcattgcg gaatacttcc ggctgggaat
       61 catgttccac gctgtacatg cacagactgc tggcgagcat gagaatcagc acaataaaaa
      121 cagaggagat gatctgattc cgcttctcat acagtaccgt cgtgatgaca ttaaatgaat
      181 catatcttgc attcagccgg aagagatgaa agattcttgc cacccttagc atccgaaaga
      241 tcacaaatcc ggacaaaaag aacaccggaa caatggtcag aatgtccacc acaccgtcaa
      301 aagacaccat aaagcgcagt ctggcgcggc atttgcgcat tcccggatat aaaagatccg
      361 ccgtccagat acggagcaca tactccacgc aaaaaatgag cagcgtgatg acctccaccg
      421 cgaaaaagaa accggacaga aagtccagtt cctcaaacgt ctgtaaaaat gtaaccagaa
      481 tattggaggc aatcgtaatc gtgataaaaa tatcgaacgc gcggctgacc cggttactct
      541 tatctccaat ctggataatg ttaaatattt tttctctcac cttaagaatc catctcccag
      601 cgtaactctg atactttcga tgcctgacgg atcctaccgg tcgtggacgg ctccgatcag
      661 ttcccggata tcctccacct gatagcgctc catgtaggcg cggattccgt caacgatctc
      721 ctccgtcgca tgcggatgat agaagttcgc cgtaccgacc gagactgcgg tcgcaccggc
      781 aagaatcatc tcgatggcat cctccgccgt ctggattcca cccattccga taatcggaat
      841 ctttaccgcc tgcgcactct ggtagaccat gcgcactgca accggatgca ctgccggacc
      901 ggacataccg cccgtgcggt ttgccagtgc aaagcatctt ctgtgaatat caatcttcat
      961 tcctgtgatc gtattgatca tggaaagcgc atccgcaccg cccgcttccg ccgcgcgcgc
     1021 catctccgtg atgtcggtga cattcgggga aagcttcatg atgaccggct gctttgcatg
     1081 gcgtttcatc tcttttgtga cttcttcgac catcttcgga ttctgtccga acgcaatacc
     1141 gccttccttt acattcgggc aggaaatatt gatctcgagc agatctaccg gctggtcgga
     1201 aagccgctcc accacatcaa tgtattcttc cgtcgtatgt ccgcagacat tgacgatgat
     1261 cttcgtgtca tattttttga ggaacgggat gtccctgtca atgaacaggt caatgcccgg
     1321 attctgtaaa ccgatcgcat tgagcatgcc gccatagacc tccgccacac gcggcgtcgg
     1381 atttccttcc cacggcacat ttgcgacgcc cttagtcaca accgctccca acttattcaa
     1441 atcgacaaac tccgaatact cctccccgga accgaacgtg ccggatgccg tcataaccgg
     1501 attcttcagt gcgacaccgc acagatcaat gccaagattc atcagaaatc cacctcctct
     1561 gcccgaaata ccggtccttc cttacagact ctcttgttct tcacattgga atgcgcatcc
     1621 ttctctgtgg acttgcagac gcaggcaagg caggcgccga tgccgcacgc catgcgctcc
     1681 tccatggaga tccaacattc gatgtcttcc tttgcggcat actcctttaa ggcgcggagc
     1741 atcggaagcg gtccgcaggc gtagatcaca tccgcggtca gcccgttctc gcggatggcg
     1801 tcgagcacat tgccctccgt tcccacactg ccgtcctccg ttgcgatata gacctctccc
     1861 tgtttgcgga actcatccag cagaaacagc tcatcccggt atccaagcac aatctgtttc
     1921 ccgcagttta actcttttgc gagctctaac atcggcggaa tcccaatgcc gccgccgatt
     1981 aaaaacgcct ttttctcctg cttcggaaag ccgttgccga gtggtcctat caccttaagc
     2041 tgcataccgg tgtgcatgga agaaaactct tcggttccct ttcccgccac gcggtatacc
     2101 agacggagcg cgccgtcctt tctgtcgatc tcacagatac tgatcggtct cgggagcagc
     2161 ctgctgcctt ccttacagta gaccgataca aactgccctg cctttgcatg cgctgcgatc
     2221 tgctcggtgt gcagccacat gctgtaaata tcgtccgcaa tctcttcctg tttgattatg
     2281 attgccgtct cttcaaattt ctgttgtgtt gtatgcatga tcttttattt ttccttatct
     2341 tcatatacca cttgtccgtt cacaagtgtc attcgcactt taccggtgac ttttctgccg
     2401 tcaaaggggg tattcctgcc cttgctgaaa aacgtgttct tgtcaatcac gtacgtctcg
     2461 tccgggtcaa aaataacgat gtctgcgacc tttccaggct caatatctcc cttgtcgatt
     2521 ccgatgatct tcgccggatt gtaactcatc ttctccgcca tctgcatcgg cgtcagatac
     2581 ccgccgagta caagctccgt gtacgttaag catgcggcgg tctcaagacc cacaataccg
     2641 aacggtgcgg atttcatgga ggtattcttg tcctcaaacg tgtgcggcgc atggtcggtt
     2701 gcgatcacat ccatcacgtc atcccgcagt ccctctttta acgcctgtac atcctccttc
     2761 gtgcgcagcg gcggattcat cttgtaattc gtgtccgcat ccgcctcgat cgccactttt
     2821 ttctccgtat ccacggtcgg ctcgatcttc ctgatgtcat cggaagtcag tgtaaaatga
     2881 tgcgggcaca cctctgccgt cacatggatt ccctccatct tagcatgctt gaccatgctg
     2941 gcagaatcct tcgtggagca gtggcacaag tgcagtctcg ccccggtatc ccggcttaac
     3001 atgatgtccc tggcaatgat gatgtcctcc acggaattcg tgatgccgcc aagtccaagc
     3061 tctctggcat tgacatcctc attcatgaca ccgccgttga caagattgat gtcctcgcag
     3121 tgagcaagca ccgcaatgtt gtacttcgca gcaagttcca tcgcctctct gtaaagctgt
     3181 gcattcatga cggatttgcc atcctcactg atggcaggaa ttccagcttt caccatgccc
     3241 tcgatgtcgg aaagctcctt tccctgctgt cccttcgtca ccgcaccgac ctgcagcacg
     3301 ttggcagcgc cgacggtctt tgctttgttg tgcacatagt tcacaacatc cggattatcc
     3361 acgaccggct tggtgttcgg catggcaaga atcgtcgtaa atccgccgtg cactgctgcc
     3421 cttgcgcctg tctcgatcgt ctccttctgt tcaaatcccg gatctcttaa atgcacatgc
     3481 atgtcgataa accccggcat gacaaagcag cccttcgcat cgatcacgcg gtcgcatttt
     3541 tccttgatac tcttctcagt tctggccaca accccgttct ccaccagcac atccagtgtc
     3601 tcatccgttc cggtggcagg attgattacg cgtccatttt tgatcaatat ggtcatcttt
     3661 ttttcctcca ttgtatgagt tcatcttatc atatccgcct acagttttcc atacaattcc
     3721 agcggcacat acgccttcac atagatcccc tccgggcggt aatcctccgc taaaagctct
     3781 ccctgctttc tgatctgctg caggattccg gcatccgcat aggcgatggt tcgctcaatc
     3841 agaactttgt tctcgcgcag aagttccgca agcagttctt ttaactcatc taaccccgtt
     3901 ccctctcttg ccgagaccgc cagggtatga tctgccctca gatcatgcag cggctcccgc
     3961 tccgtcaccg cgtcctgctt gttaaaaagc gtgatgaccg tcttttcccg gatatccagc
     4021 tgatagagcg tgtcataaac aatgtgcatc tgcttctcgt gctgcgggtt ggaagcatcc
     4081 accacatgca gaatgtagtc cgcgtatttt gcctcctcga gcgtactctt aaacgcctcg
     4141 atcagatggt gcggcagctt ccttataaaa ccgaccgtat cggtcagaag aatctcctgt
     4201 ctgcccggaa gttctaagac gcgcgttgtc gggtcaagtg tggcaaacag cttgtcctcc
     4261 tctaacaccc cggcgtcggt aagccggttc aacagtgtgg actttccggc attcgtgtag
     4321 ccgacgatcg ccgccacagg catcccgttt ctcgtgcgct tcgcgcgtgt gatctcgcgg
     4381 tgctggcgca cctcctttaa ttcccggttc agctgtgcga tgcggtcttt gatgagacgt
     4441 ctgtccatct ccagcttttt ctcacccggc cctctcgttc cgattccgcc gccgagtctg
     4501 gacatggaac gcccgaggcc ggtcagacgg ctgaggcggt attttaactg cgccagttcc
     4561 acctgtattt ttccctcgct cgtcgtggcg cgtgccgcaa agatatccag gataatcagc
     4621 gtgcggtcca tcaccttggt gtctaacatc tgctctaagt tcttaagctg tgccggtgaa
     4681 agttcatcgt cacagacgat gccggtcgcg ccccgctctg cgatcatcgc cgcgatctcc
     4741 gccacttttc cggtgcccac ataggtgccc gggtgcatca gttccctctt ctggatcagt
     4801 gtgccgacca cgacagctcc cgctgtcctg acaagctccg ccagctctgc cacagagtcc
     4861 tcggcatcat ccccatcctg ctcgctcacg ccgaccagaa tcaccttttc gatctgatct
     4921 tccaattcaa taatttctgc catgctgctc cttttctact gtccgctgtc tccgcttccg
     4981 tctgttccgg catcggttcc gctgtcggtt ccatctgcac cgtctgttcc gtccgttccg
     5041 ctgtctgctt ctgctgttcc gatatcctcc gtcacgacca cttccccctg cacttctgtg
     5101 cccgccggcg tatccacccg gcttttaaga aatgtgtatt cacgcagtgc ctccgcatca
     5161 tcagggtacg cggctgtata agcggtcatc acctcataag ctcccgcaaa atctccggta
     5221 tactccatgc agataatctg attctgcagc agcgcgcgcc tgttcggaac actctccatc
     5281 gaaagcccct gctcgatgta ggaaagcgca ccgctataat tcagcttgcc catctcaatc
     5341 tgtgccaatg cattcatcgc ttccgaatcc gcgacacccg catccacata agcctgataa
     5401 tacgtctgcg cgttcgccgt gtcctgctgc gcctcatata cctgcgcaag cgtcagattc
     5461 gcctccgcac tgcccgcatc gatcgcagac gtcaattctt tgattgcatt gtcatactgc
     5521 cccagcaggt agtaaatctc ccctctgtat gcaagacttt ccgcatcatt tcccttgatg
     5581 gaaaacgcat cgtttaagta cgccgtgccg tcctccgcca gatcataggc ggcaagattc
     5641 ttgtaaatat tgatataaag ttcataatcc gaatggttat acttgaccgc gtccgcaaaa
     5701 tcctgcttcg ccccgtccgt atccccggtg ccaagcttta aacatccgcg cagatagtaa
     5761 gcattggcgt tcttcccgtc ataatcaatc atggcgttgt acgtcccaag tgctccctca
     5821 gtgtcctgtg cggcatactg ggctgccgcc ttgtaatagc agatgtcgat ctccagatcg
     5881 gtaatttttc cggtgcagca cgcaagcgct ccgtcaaatg ccgccagtgc cccctcataa
     5941 tccccgctct gcatgctctc tatgccaatc ttgcggtagg aaagctcatc ctccgtgcgc
     6001 tcgctcgcac atccggtaag aagtgcggct gtcacaagca aaaacaccgc cgcttttccc
     6061 ctgtatgtcc tatggtgatt gatttccatt cgtcttctca tcttaattcc tctttttact
     6121 ataatgaagc caccaaaaag tataaacgat attttccttc cccgcaaggt gatttcccgc
     6181 tgcaacaagc gatatcttcc aggtacatga cttttctatc tcatgccgtc ccggatcacg
     6241 cgcgccgtgt ttttatatgc aatctgctcc acggcgtctt cggagagtcc tctctttcgc
     6301 agcgcatcga aaagcagcgg cagtttcgtg cagtcactga tctcaaattt gcctgagatt
     6361 ccgtcaaaat ccgtgccgag tcccacacat tcgataccgc ccctgtcaat cagatgcatt
     6421 gcgtggtcgc agatgcgctc gatacggctc accttatccg tctcgtcctc attcagaaag
     6481 gtcggttcca gattcagtcc cgccactccg ccacactctg ccagttcgcg gatcattgcg
     6541 tcagtaagat tgcgcgttgc cggcgccagt gcccggcagt tggaatgtga tgccacgaaa
     6601 ggcacgccgc ttcgtctgga cgtttccgcg acatcccgaa aaccgccgtc agataaatgg
     6661 gagacgtcga ctaagattcc cagttcctgc atataactca ctgcctcttt tccaaatccg
     6721 gtcagcccgc ggctcatctc ctccggcttt ttggaatgac agtgtcccag acagttgtcg
     6781 tcattccagg tcagtgtcac aagtcttacg ccgagatcgt aaatttcccg gattttgtcc
     6841 attttcccat caatcagagc accgttctcg atcgtgagaa acgccgatat tttcccttca
     6901 ttctgattct gctttaattc ctctgcattg tgcgctgcag caaagcggtc accgtactgc
     6961 tccatggtat ggcagaaaat gtcatacatt ttcctcatga gtacttccgc cgacggcatc
     7021 tcttctaagc cgaaccagtc cggatcattt ctctgcggca gaaacatcgc aaaaaactgc
     7081 gcacctgcct ctgcctgctg caggcgcagt ccatccacca tcgtgcggtc aagttccaga
     7141 aacgtctctg ttttctgcgc taacgcgctt cccaatgtat cacaatgcat gtcaatataa
     7201 cgcatcattt tctccatttc cgggcagagt tccttcttag ttttccggac tgctgccctg
     7261 tctttttctt attataaaga acccgcggac tttatacaat atgccggccg ccggattttg
     7321 tacagcattt tctctgtttc cactgttttc atgcaaaaag gagcgccttg cggcactcct
     7381 ctctgtgaaa gaaatatata aattttgaag caagcttttt cttccggacg gttttccccg
     7441 gttttacaga tgccagatgt cctcattgta ctgtgcgatc gtacggtcag aggagaagaa
     7501 tccggctttg ctgatgttga tgagcatttt cttcgcccac gccatgcggt cttcataatc
     7561 cgcatagatg cgctccttgg ttgccacata gtccttgaaa tccggaagcg tcatgaacca
     7621 gtctttgttc agaagttcgt tgtaaagacg ctctaagttc tctctgcatc cgactgccat
     7681 catctcgtcg ctcacgataa agtctaccgc acgcttgatg ttcgcatcat tctcatagta
     7741 gcttctggac acataatctg ccttctcgta gtgcttgatg acttcctctg aggactcacc
     7801 gaagatgtaa atgttgtcgt cgccgacaaa ctgatgcatc tccacgtttg caccgtccat
     7861 ggttccgatt gccactgcac cgtttaacat gaacttcatg ttggatgttc cggacgcctc
     7921 cttggaagca agggaaatct gctcgtggat atctgccgcc gggatcagct tttcagccag
     7981 cgtgacattg taattttcca ccatcacaac ctttaagtac ggagatacct ccggatcatt
     8041 tgcaatcaac tcggacaggc agaggatcag gtgaatgata tcttttgcaa tcacgtaagc
     8101 cggggctgcc ttggcaccga aaatcacggt gatcggacgg gtcggcttct ttcctgcctt
     8161 gatctcgaaa tatttgtgga tgacatacag cgcattcatc tgctgtctct tatactcgtg
     8221 cagacgcttg atctggatat cgtaaatgga attgtcatcc agcacgattc cctgtgtttt
     8281 tgcaagataa tctttcagct cagtctttct gctgttcttg acatcaagca ggcgctgcag
     8341 aacggaagcg tcatcgacaa gcgcgccgag cttctccagt tccatcgcat ccttcttaaa
     8401 gcccggtccg atcagggatt cgatcagatc tgccagctcg tggttgcagt gcagcagcca
     8461 gcggcggaag gtaatgccgt ttgtcttatt gttgaacttc tccggataga tcttgtagaa
     8521 gttgttgagc tccgtatttt tcaaaatttc cgtgtgcagg taggcaacgc cgttgacact
     8581 gtagccataa tgaatatcca tatgtgccat atgcacgcgt ttctcgtcat caatgatcgc
     8641 aaccgacttg tcatcgtatt tgcgtaccac gcggctgttc agttcgtaga taatcggaag
     8701 aagctgcgga actgcctttt ctaaaaaatg aatcggccat ttttccagtg cctccgcaag
     8761 aatcgtgtgg ttggtgtatg cgcaggtctt cgatacgatc tcaatcgcct catccatcag
     8821 aattccacgc tgcatcagaa gacggatcag ctccggaatc accatgctcg ggtgtgtatc
     8881 gttgatctgg attgccgcgt actcattcaa cttgtgcaga tcaccgcctc ttgccagcgt
     8941 ctcgtcgatg atgagctgtg ccgcattgct gaccataaaa tactgctggt atacacgcag
     9001 gatgcgtccc ttgtcatccg aatcgtccgg gtataagaac agcgtcaggt tctttgcaat
     9061 atcctctttg tcaaaattga tgccgtcccc tacaaggctc tcgtccacgg tctccacatc
     9121 aaacagatga agctttgtgg tacggttctc gtaaccaatc acatcgatat catacagtct
     9181 ggactgtaag gtaaaaccgc cgaactgtac cgggtaggtc acgttcgttt ttgtcagcca
     9241 gctctccttc tcgatccacg ggttcggctc ctcgtgctgt aaattgtgct caaatacctg
     9301 tttgaacaga ccgtagtgat aattgagacc cacgccgtcg ccgtttaatc caagcgttgc
     9361 aatggaatcc aggaagcatg ctgccagacg acccagaccg ccgtttccga gagacggctc
     9421 cggctcaacc tcctcgatct ctgccaggtt cttgccgttc tccgccagaa cctgctttac
     9481 ttcctcataa ataccgaggt tgatcaggtt gttggataac agcttaccga tcaaaaactc
     9541 ggcggagata taataaagct tgcgctttcc ctcgttgctc tccttttcct tcgccatgct
     9601 ctttgtcatc tcaagcagcg cgtaataaat ctcttcgttg gagcactgtg caatgccctt
     9661 accatatctg ttgtttacga tttcctgtac gttcatgata tcctccttgt tttgcgaagc
     9721 aaaatccgag tcctttgacg aggagaggat tttactcctt gttttgcatc cccaattact
     9781 ttttgtattt ccctgctgta tgagaacatt atatgcagaa gtccactttt tttcttgtca
     9841 tattatcccc aaaagtggta caatcctatc atgaacattt tagagtatga aaactatcag
     9901 gaaaaaattc cacatggcaa cccgctgttt ccttatatca cctacccttg ctcgattccg
     9961 cttgactttt cgcgcgtgcc tctgcactgg cacgatgaga tggaaatcat ctatatcaaa
    10021 aaaggaaccg gagcaatcac ggtcgatttc aaatcctacg tggtaaccga gggaaccatc
    10081 gcgctgattc tgccgggcag gctacacagc attgaacagt acatgcagga aagcatggaa
    10141 tacgaaaata tcattttcca tccgggcatc ctcatttcca aaaaaacgga caccagcaat
    10201 accgaatttc ttgcgccgct gttgtcgggc gtcctgtccg ttccgctctt atatcagccg
    10261 ggatctccct gctatgcgga gattgccgcc tgcattgacg ccaacgatga aatcagccgg
    10321 acgaacccgc ccggctatca gttctttatc aagagtcagc tctttatgct tttttacatt
    10381 ttattcagca aatgcctctt aaaagagccg gcaaagcgcg accacaaatc gctggagaag
    10441 atgaagctaa tcttaaaata tctggagaac cattatatgg agcgcattac aattgcagat
    10501 atctcggcgg aggtcgggct gagccagtcg catttcatga agtatttcaa gaatacgatg
    10561 ggcacttcct ttattgaata tctcaatgaa taccgcctga ccatggcttc ccggctgttg
    10621 atctcctccg aatcctcgat tcttgccatt gcggaggaag tcggctacga gaatctgtcg
    10681 tactttaacc gcactttcaa aaaaagattc ggcatgactc cccgggatta ccgaaaacaa
    10741 tccacgaagc agttgtaaaa cgggtcccac agaggatttt ccttgtttta gacccgtttt
    10801 tcttcatttt tccagtcaaa atggtgcttt ttctatgtaa aacgggtcca gagcagattt
    10861 tttcctctgc cagacccgtt ttttgcaaaa atccgggtcc cacagtcatt tttttctcct
    10921 gtggacccgg atacctcttt ttcacacgtc tgaacatacc atctggacag tttttctttt
    10981 tctcgtgctt tctctttctt ttttcttacg attctatatc aaagtcaatg cttcccatac
    11041 cgcagtccag atcaatcgtt cccacagccc cggcattcgt gactttgtag cttccgctga
    11101 gatcggaata gctttctccg tttaccgtga gatcccccat gccgcaggac atcttataat
    11161 tatagtcctc ttccctgccg cgcagggtga tgctgccatc ccccatgccg caatgcgctt
    11221 tcacattctc tgccacaatg ccgtccagcg caaatgatcc cataccgcaa tccagcttaa
    11281 tatctttata ggtgcctcct tccagatcaa tatcaccggc tccaagcttg atgtccaggg
    11341 ttcctgtcac agcaaaatct gctcccgtca gttctcctgc tcccacattc agctccatcg
    11401 ttctgcagtt cacactgccc gatgcaaaat ctgcatctgc cgccccgatg tcaaagtcca
    11461 tggtatcaaa gtggatattt ctgttattct taactgactt gagcttgatt ctggcatcaa
    11521 tatccattcg
//
LOCUS       DBMDQJ010000002        11477 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_2656_length_11477_cov_1.495341, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000002 DBMDQJ010000000
VERSION     DBMDQJ010000002.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 11477)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11477
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2656_length_11477_cov_1.495341"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2394
                     /locus_tag="ACZO9R_00055"
     CDS             <1..2394
                     /locus_tag="ACZO9R_00055"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911495.1"
                     /translation="KDSVGAKAAAWVGITLGAVVFFTGAAGVLLMDQAGVYEMSREQF
                     LTKSYQSVADQYAIRSLDYSVNMENGAKPENNRWNDTYFRYGIIEAENIDSVDLNESY
                     TYVERNFDEKVTRDKLYCKSYDINEVTRYDYSMTLWGGYSLYTDGGDYAAQVEIEKRC
                     YNADDGIFYYETPDAYIPVKHVSILMLSPDLATGYDYTYDADVGKYLDLHAYSGTYVT
                     FDDADSLSENTGFQTVDWTNSVILDGVTCGELVGIPVTVIGNSDLEQKGSDARTTYQL
                     TDGMLQMNYTAESLHRKYWVVTILPENVPLGWSDDLFVQVTTLTALMYGVRYAIFPIL
                     AAAFVLAVFCLVWLVRSAGHHAGEDGITLTWLDGMPLDVYLGFALVAEGFLFLILMEA
                     VNGRNAAVPMLVGVLFCLIAGGWIALLSLLTFAARVKAGAWWKNTVVYMALGVIGRLC
                     GAVTRNLPLFLRAVLVFGIAMMAEALAGTLHTGSSMGAGWRFVKVVEFACIFWGVLQM
                     KRLAEGGERLAEGDMEHPVDTTHMYRDLKEHGEHLNSIQTGMAKAVAESVKSERFKTE
                     LITNVSHDIKTPLTSIINYVDLLEKEELSNETAEGYLEVLERQSNRLKKLIEDLIEAS
                     KASTGNLAVHLEKLEAGVSVVQIVGEFEDKLNAADLTLLIQKPETPVYVMADGRHFWR
                     VIDNLMNNICKYAQPGTRVYVDLRENGGQVELIFRNTSRYPLNISSEELMERFVRGDE
                     SRNTEGSGLGISIAKSLMELMGGSFMLYVDGDLFKVVLGFAQMEEEKEPLDEDKSGK"
     gene            2568..3515
                     /locus_tag="ACZO9R_00060"
     CDS             2568..3515
                     /locus_tag="ACZO9R_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR0911496.1"
                     /translation="MRAVKKEWLLGMLCVLSGVMLMVMHPVQAQAGQADRAAYRAVFD
                     ASYYSAAYPDVAAAYGNNADALLNHFISFGVKEGRNASAEFNPQAYRQRYEDLQAAFG
                     SDMAAYCRHYVAYGKAEGRNGSADGQPVVAVQQADSQAQQTDSQTGTQNNGTAQSSAP
                     AQSTQQASGTVIGTCTTSYVDNIPRATNVALAAQRINGTVVQPGGSFSFSNTILPRTS
                     ANGYVTAPIYVSGKKGTGTGGGVCQVSSTLYAAMVYAGLPATERHAHSLPVDYLPAGL
                     DATIAGSYLDLKFTNTFSQPLLIQASANGGTLTVTLVLQ"
     gene            3863..4144
                     /gene="flgM"
                     /locus_tag="ACZO9R_00065"
     CDS             3863..4144
                     /gene="flgM"
                     /locus_tag="ACZO9R_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HIR0911497.1"
                     /translation="MRVEAYNSVAQIYKTNRAAGASAAAKTEKSRDQVEISSIGYDYQ
                     IAKQAVAETSDIREDKVAALSAKVKSGTYDVSNEAFAEKLLEKYRSFML"
     gene            4168..4680
                     /locus_tag="ACZO9R_00070"
     CDS             4168..4680
                     /locus_tag="ACZO9R_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HIR0911498.1"
                     /translation="MASLMEELLGVLEKEETEYLTLIDLADVKSQAIIKADIAKLGEV
                     TEKEQEAASTLLNLSNKRTQVLNDMATVLGKKPEQMTINRMIGYLENQPREQQMLAER
                     RDRLLEVGTKMQTLNHQNEALLKQAMEMVEFDLTLLKSMRQAPETANYNKNAYNTGDL
                     LGGSGFDAKQ"
     gene            4706..6523
                     /gene="flgK"
                     /locus_tag="ACZO9R_00075"
     CDS             4706..6523
                     /gene="flgK"
                     /locus_tag="ACZO9R_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HIR0911499.1"
                     /translation="MANGFGTLFIGVSGLQSSQNALNTTANNLANVDTTGYVRQQVLF
                     TDRNYVTFNETTAVSKQQSGLGVYIGDVIHSRDVFLDRSYRTESGRQAFYAATYEATN
                     EVETYFQEMEGQAFQDALEDFWTSFQELYKTPDDSVSQNLVVQKAQLFVSRANAVYSG
                     LQSYQYNINTQISDNIDQVNKLGDTIQDLNLQIMKVEAGGIETAMTLRDARDQALDEL
                     SALVDISYNETADGIVKVSIEGTEFIDESRCYHIEKKTDKLTGFITPYWGYLSDVNKG
                     KYVNVFDFGSRDISTENKNDMGELKALVLARGDHIANYTDIEGLDQDTYNDTTGMSVM
                     LRAEAQLDQMIHGIVTALNDVLCPNVTAEDTIKNLTNGAATTLDVILADGSTVTLNAN
                     TKILDADNCATGSDKQLPPQELFSRIGTERYTKATYTYQPVDENGNPKVDANGNPVTE
                     TKEIYIYNEEDPNDTTKQYTLQSLSVNEALVIDETLLPHLCQNGDVDYALAAKLAGVW
                     DEERLTLNPNDTKACSFKDYYKDMIGEMATYGNVYNSTATSLTGTVASVDNQRQQVIG
                     VSSDEELTYMIKYQNAYNAASRFINVVNEMIEHLLTQLG"
     gene            6555..8396
                     /gene="flgK"
                     /locus_tag="ACZO9R_00080"
     CDS             6555..8396
                     /gene="flgK"
                     /locus_tag="ACZO9R_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HIR0911500.1"
                     /translation="MPSTFFGLNIASSGLSAYQVALNTTANNIANVQTTGYSRQQSNR
                     VASDALLVHQQYGAVGSGVTTLSVKQIRNQYYDTKYWYNQSSVGLYETKLSYLQQIEN
                     YFIDDSSSKGFTTILNTMFNSLDTLKNNASDVNTRQQFIGSAQNLATYFNSVSEGLTD
                     IQKGTNDEIKSTVQNINAIAEKIAVLNKQINVIEIQGGYANELRDQRALLIDELSEIV
                     PTEVSEVPITDTNHPDEPTGANYYTVKIGGQVLVDTYNYETLECKAREYKVNQTDAAG
                     LYDIKWSKTGNTFNAGGSSMSGTLKALFDIRDGNNGENFSGLAKVLNSTEVQITNPSI
                     TSIVGVSMPEEGILTINGREYAYEGFTFEQDADGNITSYTFNMKRALTAEEQARVDGR
                     TASVGTSIDAMGIPYYMSQMNQFLRSFATEFNNILKKGVDLDGNSTSGYAFFTGTDTV
                     TGEEFSFDGKDANGNAVNGSGSDTYYKLTAANICVNSMIVKDPTKLATTVDINGKGGV
                     DAYDLVEELATLKSGKVLYRGGTADGFLKCMIADISVDTQQSEIFSKNYQNVASAIDT
                     QRMSISGVDEDEEAMDLIKFQNAYNLSSKMVSVMAEIYDRLILETGV"
     gene            8422..9993
                     /locus_tag="ACZO9R_00085"
     CDS             8422..9993
                     /locus_tag="ACZO9R_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin N-terminal helical domain-containing
                     protein"
                     /protein_id="HIR0911501.1"
                     /translation="MRITNNIILHNTTGNINGNKVNVNNLNNQMTSQKKIQRPSENPV
                     IAVRSLRLRTTLSEIDQYYENNIPDAESWMKVTETALANMKTILTDIRTQCTYGASDQ
                     ITADDRKTILTQLEKLRDQVYAEGNADYAGRTVFTGYRTNQKLTFMTDDNTTSYNITQ
                     GLSYKNLEEHRYYSDEVTLPTTATEVTGNTISNPKQATFDRIRLSYGEIDSITDKDGN
                     ALSANGATGTLSYSYTDAAGVTQTDGELKVTVYDTFEDWAKASQTADNTYNITDGEAV
                     FIKESGELILSSDASSTIKSGKASLDVNYTKTGFNKGEVRPEYYYNCTNITDAANPVE
                     YIKFENGKEIYQDINYVVAANQTLTVNTQASAVFDASIGRDVDAMIEAVKFAQDANNK
                     VEELEKMQKMQEYSSDDCQAALEDWIAAAKKERDYADDNLQKLYNSYIGNFDDYLQKV
                     NLANTDLGSKGQSLDLTKNRMANQQTTMEELKSTNEDRDLSEIIIDYASTYTAYQASL
                     QAAAKINQTTLLNYI"
     gene            10027..10488
                     /gene="fliW"
                     /locus_tag="ACZO9R_00090"
     CDS             10027..10488
                     /gene="fliW"
                     /locus_tag="ACZO9R_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HIR0911502.1"
                     /translation="MKANTRLFGEIDIADDKIISLEDGMIGLPEYRNFALIFDEEKEK
                     DKSSVMWFQSMDDPQTAFPVMQPNHVCPDYNPMVSDEMLRPLGGMTEDNTYVLVTLTA
                     APDPKDTSVNLKAPIVINTDTRRGAQIIVEDDYPVKYKIYDVVKGKKKAEE"
     gene            10492..10710
                     /gene="csrA"
                     /locus_tag="ACZO9R_00095"
     CDS             10492..10710
                     /gene="csrA"
                     /locus_tag="ACZO9R_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HIR0911503.1"
                     /translation="MLALTRKKGESLVINNNIEITVLEIRGDQVKIGISAPKEVPVYR
                     KEVYLQIQNENKAALNATDAMEALKNLF"
     gene            10801..11229
                     /locus_tag="ACZO9R_00100"
     CDS             10801..11229
                     /locus_tag="ACZO9R_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HIR0911504.1"
                     /translation="MEIESMKSGAAASYQGSAAASTKTLDAAVQPEASTQAGLSQTAD
                     KKPTVQNFFTEKDQEDKDLSEEKDAKQGTQLKKAVNDLNKQMKNSEAIFGIHDKTNRV
                     TIKIIDKTTKEVIKEYPPEETLDMIAKVWEIAGILVDEKL"
     gene            11247..>11477
                     /locus_tag="ACZO9R_00105"
     CDS             11247..>11477
                     /locus_tag="ACZO9R_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HIR0911505.1"
                     /translation="MPIRITGLNSGLDTEAIISALVSSYNYKTNKYKKAQTKLSWKQA
                     AWKTLNTKIYSLYSSVGNLKLSSAYNLKSTTVS"
BASE COUNT         3285 a         2508 c         3251 g         2433 t
ORIGIN      
        1 aaagacagtg tgggagcaaa agcggcagcg tgggtcggca tcacgctcgg tgcggtggta
       61 tttttcacag gggctgccgg cgtgttgctt atggatcagg cgggcgtgta tgagatgtcg
      121 cgcgagcagt tccttacaaa gagttatcag agtgttgcag atcagtatgc catccggtcg
      181 ctggattaca gtgtgaacat ggaaaatggt gcaaaaccgg aaaataatcg gtggaatgac
      241 acgtatttcc gctatggaat cattgaagcg gaaaatattg attccgtaga tctcaacgaa
      301 tcgtacactt atgtggagcg caattttgat gagaaggtca caagggataa gctgtactgc
      361 aaaagctatg acatcaatga ggtgacacgc tacgattaca gcatgacgct gtggggcggg
      421 tacagcctct acacggatgg cggagattac gctgcgcagg tggagattga gaagcggtgt
      481 tacaatgcgg atgacggcat attttattat gaaacgccgg acgcatacat cccggtaaaa
      541 catgtgagca ttctgatgct gtcgccggat ctggcgacag gctatgatta cacgtatgac
      601 gcagatgtag gaaaatatct ggatctccat gcgtacagcg ggacctatgt gacattcgac
      661 gatgcagaca gtctgagcga aaacacggga tttcagacgg tcgactggac gaacagtgtg
      721 attctggacg gagtcacctg cggtgagctt gtgggaatac cggtcactgt aatcgggaac
      781 tctgatctgg agcaaaaggg aagcgatgcc aggacaacgt accagcttac tgacggcatg
      841 ctgcagatga actacacggc ggaatcactg caccggaaat actgggtagt cacgattctt
      901 ccggagaatg tgccgcttgg ctggagtgac gatttgtttg tgcaggtgac gacgcttacg
      961 gcgctgatgt atggcgtccg ctatgccatt ttcccgattc tggcggcggc atttgtgctt
     1021 gccgtattct gcctggtgtg gctggtgcgg tcggcggggc atcacgcggg agaggacggg
     1081 attacgctta cctggctgga tggcatgccg cttgatgtgt atctcggatt tgcgctggtg
     1141 gcggagggat ttctgtttct gatcctgatg gaagctgtca acggcagaaa tgctgcagtg
     1201 ccgatgcttg tcggggtttt gttttgcctc atagcgggag ggtggattgc gcttctgtcg
     1261 ctgctgacct ttgctgcgcg cgtgaaagcc ggggcatggt ggaaaaatac cgtcgtctac
     1321 atggcgcttg gcgtgatcgg caggctttgc ggcgcggtga cccggaatct gcctcttttc
     1381 ctaagggcgg tgcttgtttt tggcattgcc atgatggcgg aagcgcttgc cggcacgctt
     1441 catacaggaa gcagcatggg agcgggctgg cggtttgtaa aagtcgtaga gtttgcatgc
     1501 atcttctggg gagttttaca gatgaagcgt ctcgcagagg gaggcgaacg ccttgcggag
     1561 ggcgatatgg agcacccggt cgatacgacg cacatgtacc gggatttaaa ggaacacggc
     1621 gagcacctca acagcatcca gacgggtatg gcaaaggcgg ttgccgagag cgtgaagagt
     1681 gagcggttta agacggaact catcacgaac gtctcgcacg atattaagac gccgcttact
     1741 tccatcatca attatgtgga tcttctggaa aaagaggagc tttccaatga gacggcagag
     1801 gggtatctgg aagtgttaga gcgtcagtca aaccggctga aaaagctgat tgaggatctg
     1861 atcgaggcgt cgaaagcttc cacgggcaat ctcgccgtac atctggaaaa actcgaggcg
     1921 ggtgtttctg tggtgcagat cgtcggagaa ttcgaggaca aactgaacgc cgcagatctg
     1981 acgctgctga tccagaagcc ggagacgccg gtgtatgtga tggcggacgg cagacatttc
     2041 tggcgcgtga ttgacaacct gatgaacaat atctgcaaat atgcacagcc gggcacgcgg
     2101 gtatacgtgg atctgcggga gaatggcggg caggtggaac tcattttccg gaatacgtcg
     2161 cgctacccgc tcaacatatc cagtgaggaa cttatggagc gttttgtccg cggggacgaa
     2221 tcgcgcaata ccgagggaag cggactcggt atctccattg caaagagcct gatggaactg
     2281 atgggcggca gctttatgct ctatgtggac ggcgatctct ttaaggttgt gcttggattt
     2341 gcacaaatgg aggaagaaaa agaaccactt gatgaagata aaagcggaaa atagatacaa
     2401 atgcaatttt tctgacactt tctgaaaact ttgcttgttt tttgataaac cctctgttac
     2461 aatgcaaaaa aagacagaac cgcaggaaac ggaaagaatg cacacaggag aaagacagaa
     2521 ggaaggcggt ctgtgaggaa actgtggtta cgttcggagg aagagttatg agagctgtga
     2581 aaaaggaatg gttattagga atgttgtgtg tgctgtctgg cgtgatgctt atggtgatgc
     2641 atccggtaca ggcacaggca gggcaggcgg atcgcgcggc gtaccgtgcg gtgtttgacg
     2701 catcctatta cagtgcggcg tatccggatg tggcggcagc ctatggaaat aatgcagatg
     2761 cgcttctgaa ccatttcatc agctttggcg tgaaggaagg ccgcaatgcc agcgcggaat
     2821 ttaatccgca ggcatacaga cagcgttatg aggatctgca ggcggcgttc ggcagcgaca
     2881 tggcggctta ctgcagacat tatgtggcat atggaaaagc cgagggcaga aacggcagcg
     2941 cggacggaca gccggtggtg gcagtacagc aggcagacag ccaggcgcag cagacggaca
     3001 gccagactgg gacacagaac aatgggacag cgcagagcag tgcaccggca cagagcacgc
     3061 agcaggcatc cggcacggtg atcggtacct gtacgacttc ttatgttgac aatattccgc
     3121 gcgcaacaaa cgtggcgctt gcggcacagc gcattaacgg cacggtggta cagccgggcg
     3181 gcagcttttc tttcagcaat accatcttac cgcgcacatc tgcgaacgga tatgtgacgg
     3241 ctccgatcta tgtgagcggc aaaaagggca ccggcaccgg cggcggtgtg tgccaggtat
     3301 cgtccacact gtatgcggcg atggtatatg cgggacttcc ggcaacggag cgccatgcac
     3361 attcccttcc ggtagactat cttccggcgg gacttgacgc gacgattgca ggcagctatc
     3421 tggatctgaa gtttacgaat acattttcac agccgctgct gattcaggca tcagcgaatg
     3481 gcggaacact gaccgtgaca ctcgtattgc agtgattgcc attgcgcagt gaattggctg
     3541 atacacagtg aaacggtttt tatacagcga attggctgat gcacagtgaa attgtttttg
     3601 cacagtaatg gtatctgcgg gtaagcaggt gcttgtattc aaatgaatgg attcatatac
     3661 attattttgc aggggattgc atctgcgcct taagaatgac tctgagggca gatcaatgcc
     3721 cttgttttgg tgccgtcaat gatgaaaata agacgaaatc tttttatctc ataaaaatta
     3781 tcactaagga aaaaaccaaa tcatccgata tattgactag aaaagttatt tttgcgttac
     3841 agaatagaaa ggagatgcca ttatgcgagt agaagcgtat aacagcgtgg cacagattta
     3901 taagacaaac agagcagccg gggcatccgc agcagcaaag actgagaaga gccgggatca
     3961 ggttgagatc tcttccatag gatatgatta tcagatcgca aagcaggcag tagccgagac
     4021 ttctgacatc agagaggaca aggtggcggc gctctccgca aaggtgaaat ccggcactta
     4081 cgatgtgagc aacgaggctt ttgcagagaa gcttctggag aaatatcgtt cctttatgct
     4141 gtaggataag agaaatgagg gcagattgtg gctagtttaa tggaagaact tctgggagtt
     4201 ctggaaaaag aagaaacgga atatctgacg ctgattgatc tcgcggatgt gaagagccag
     4261 gcgattatca aagcggacat tgcgaagctt ggcgaggtga cggagaagga gcaggaggcg
     4321 gcgagcaccc tgttaaacct gtcgaacaag cgcacgcagg tgcttaatga catggcgacg
     4381 gttctcggga aaaagccgga gcagatgacg atcaaccgta tgatcggtta tctggagaac
     4441 cagcccagag agcagcagat gcttgcggag cgccgggaca gacttttaga agtgggaacg
     4501 aagatgcaga cattgaacca tcagaacgag gcccttttaa agcaggcgat ggagatggtg
     4561 gaatttgacc ttacgctgct aaagagcatg cggcaggcgc cggagaccgc taattacaat
     4621 aaaaacgcct ataatacagg agacctgctt ggcggcagcg gatttgacgc gaagcagtag
     4681 atagagcagc acggaggaag ataccatggc aaatggtttt ggaacattgt ttatcggcgt
     4741 gtccggcttg cagagcagcc agaacgcatt aaatacaacg gcaaataacc ttgcaaacgt
     4801 agataccacg ggatatgtac gtcagcaggt actgtttacc gaccgcaact atgtaacctt
     4861 taatgagacg acggcagtca gcaagcagca gtccggtctg ggtgtttaca tcggtgatgt
     4921 gattcattcc agagacgtat ttctggatcg ctcttaccgc acggagtcgg gacggcaggc
     4981 attctatgcg gcaacttatg aggcgaccaa cgaggtcgag acttattttc aggagatgga
     5041 gggacaggct ttccaggatg cactggaaga tttctggacc tcttttcagg aattgtacaa
     5101 gacgccggat gacagcgtga gccagaatct ggttgtccag aaggcacagc tgttcgtgag
     5161 ccgcgcgaat gcggtttatt cgggattaca gagttaccag tacaacatca atacgcagat
     5221 cagcgacaat atcgatcagg tcaacaagct cggcgatacc atccaggatc tcaaccttca
     5281 gatcatgaaa gtagaggcgg gcggcatcga gacagccatg accctgcgcg atgcgagaga
     5341 ccaggcgctg gatgagctgt ccgccctcgt ggatatttcc tacaacgaga cagccgatgg
     5401 tattgtgaaa gtcagcattg agggcacgga gtttatcgat gagagccgtt gctatcatat
     5461 cgagaagaaa acagacaagc tgaccggttt tatcacaccg tactggggct acctttcgga
     5521 tgtgaacaag ggaaaatacg tcaacgtatt tgattttggc agccgggata tctccacaga
     5581 gaacaagaac gatatgggtg aactgaaagc gctggtactg gcgcgcggcg accatatcgc
     5641 gaactatacg gatattgaag gactggatca ggatacctat aacgatacga ccggaatgtc
     5701 cgtcatgctg cgggcagagg cacagctgga tcagatgatc cacggcatcg tgaccgcgtt
     5761 aaatgatgtg ctttgtccga atgtgactgc ggaagatacg attaagaatc ttacgaacgg
     5821 tgctgctaca acgctggatg tgatactggc ggacggctcg accgtaacat taaatgcgaa
     5881 caccaaaatt ctggatgcag acaactgcgc gaccggttcc gacaagcagc ttccgccgca
     5941 ggaactgttc tccagaatcg gaacagagcg gtacacaaag gcgacttaca cctatcagcc
     6001 ggtagatgag aacggcaatc cgaaagtgga cgccaacggc aacccggtga cggagaccaa
     6061 ggagatctac atttacaatg aagaggaccc gaacgatacc acaaagcagt acacactgca
     6121 gagcttaagt gtcaacgagg cgcttgtgat cgatgaaacg ctgctgccgc atctatgcca
     6181 gaacggtgat gtcgattatg cactggcagc aaagctggcc ggtgtgtggg atgaggagcg
     6241 gcttacgctg aacccgaacg acaccaaagc ttgcagcttt aaggattact acaaagatat
     6301 gatcggtgag atggcaacct acggaaatgt ctacaattcc acggcgacca gcctcacggg
     6361 aacggtggct tctgtggaca atcagagaca gcaggtcatc ggcgtatctt ccgatgagga
     6421 gctgacctac atgatcaagt atcagaatgc ttacaatgca gccagccgtt tcatcaatgt
     6481 ggtcaatgag atgatcgaac atctgctgac ccagcttgga taattgagcg cgtaatagaa
     6541 agagagggtg ttacatgcca tctacatttt ttggtttaaa tattgcatca tcaggcttaa
     6601 gtgcctatca ggtcgcactg aatacaacgg ctaacaatat tgcaaacgtg cagacaaccg
     6661 gctattccag acagcagtcg aaccgtgtgg catcggatgc gcttttagtg caccagcagt
     6721 acggagctgt gggaagcggc gttacgacct tatctgtcaa gcagatccga aatcagtatt
     6781 acgatacaaa atactggtac aatcagtcat cggtcggact gtatgagaca aaattgagct
     6841 acttacaaca gatcgagaac tattttatcg acgacagctc atccaagggc tttacgacga
     6901 tcctgaacac gatgttcaat tcactggata cattgaagaa caatgcatcg gatgtcaata
     6961 cccgccagca gtttatcgga agcgcacaga atctggcaac ctacttcaac agcgtgtcag
     7021 aaggactgac cgatattcag aaggggacga atgatgagat caaatccacg gtccagaata
     7081 ttaacgcgat cgcggagaag atagcggtat tgaacaagca gatcaacgtc attgagattc
     7141 agggcggcta cgcaaacgag ctgcgcgacc agcgagccct gttgattgat gagttgtccg
     7201 agattgtgcc gaccgaggtt tccgaggtgc cgatcacgga tacgaaccat ccggacgagc
     7261 cgacaggtgc caattactac actgtaaaaa tcggtggtca ggtgcttgta gatacctaca
     7321 attacgagac gctcgagtgc aaggcgagag agtataaggt caaccagacg gatgcagccg
     7381 gactttatga tattaaatgg tcgaagacag gcaatacctt taatgcgggc ggaagctcga
     7441 tgtccggcac gttaaaagcg ctgtttgaca tccgcgacgg aaataacggc gagaatttct
     7501 caggactggc aaaggtgtta aacagcaccg aggttcagat taccaatcct tccatcacga
     7561 gtattgtggg agtcagcatg ccggaggaag gcatattgac gatcaacggt agagagtacg
     7621 catatgaggg atttaccttt gagcaggatg cggacggcaa catcacttcc tataccttta
     7681 atatgaagag agcgctcacg gcggaagagc aggcacgtgt agacggacgc acggcatcgg
     7741 tcggaacgtc gattgacgcg atgggtattc cgtattatat gagtcagatg aaccagttcc
     7801 tgcgcagctt tgcgacggaa ttcaacaata ttttgaaaaa aggtgtcgat ctggacggca
     7861 attccaccag cggatatgca tttttcaccg gtacagacac cgtgaccggc gaagagtttt
     7921 ccttcgacgg caaagatgcg aacgggaatg cagtcaacgg atctggctca gacacgtatt
     7981 acaagctgac agcagcaaat atctgtgtga acagtatgat tgtcaaggat ccgaccaagc
     8041 tggcgacaac cgttgacatc aacggaaagg gtggcgtgga tgcgtacgat ctcgtggagg
     8101 aacttgcgac gttaaagagc ggcaaggtgc tttaccgcgg aggtacggcg gacggattct
     8161 taaaatgcat gattgcagat atttccgtgg acacccagca gtcagaaatt ttcagcaaga
     8221 attaccagaa tgtggcgagc gcgattgaca cgcagcgtat gtcgatttcg ggcgttgatg
     8281 aagatgagga ggcaatggat ctgatcaaat tccagaatgc ctacaatctt tcttcaaaga
     8341 tggtatctgt catggcagag atttatgaca gactgatcct tgagaccggt gtatagacaa
     8401 ggacatgagg aggaatcata aatgcgtatt acgaataaca ttatacttca caatacgacg
     8461 ggcaatatca atggaaataa agtgaatgtc aacaatctga acaatcagat gacatcccag
     8521 aaaaagattc agagaccttc cgagaatccg gtcattgcgg tgcgttcact cagactccgc
     8581 acaacactca gcgagattga ccagtattac gagaacaata ttccggatgc agaatcctgg
     8641 atgaaagtga cagagacggc acttgccaat atgaagacga ttctgacgga catccgtaca
     8701 cagtgtactt acggagcatc cgatcagatt accgcagatg accgcaagac gatcttgacc
     8761 cagttagaga agctgcggga tcaggtctac gcggagggaa atgcagatta tgcgggacgt
     8821 accgttttta ccggttaccg cacgaatcag aagctgacct ttatgacaga cgacaatacg
     8881 acatcttaca atatcacaca gggactttcc tataagaatc tggaagagca ccgctactac
     8941 agcgatgagg tgacacttcc gaccacagcg acagaagtga ccggcaacac catctcgaat
     9001 ccgaagcagg cgacattcga ccgtatccgt ttaagctacg gcgagatcga cagcatcacg
     9061 gacaaggacg gtaatgcgct gtcggcaaac ggtgcgaccg gaacgctcag ctattcctac
     9121 acagatgcag ccggcgtgac acagacggac ggcgaactga aggtaaccgt atacgatacg
     9181 tttgaggact gggcaaaagc atcccagact gcggacaaca cctacaatat cacagacggt
     9241 gaagcggtat ttattaagga gagcggtgag ctgatcttaa gctctgacgc gtcatcaacg
     9301 atcaagagcg gcaaggcaag cctggatgtc aactacacca agaccggatt taacaaaggc
     9361 gaggtacgcc cggagtatta ttacaactgc accaacatca cagatgcggc aaatccggtg
     9421 gaatatataa agtttgaaaa cggcaaggaa atctatcagg atatcaacta tgtcgtagcg
     9481 gcaaaccaga ccctgacggt caatacacag gcatccgctg tatttgatgc atcgattgga
     9541 agagacgttg acgccatgat cgaggcggta aagttcgcac aggacgcaaa caataaagtc
     9601 gaggaactgg agaaaatgca gaaaatgcag gagtattctt ccgatgactg tcaggcggca
     9661 cttgaggact ggatcgcagc ggcgaagaaa gaacgcgatt acgcggacga taatctgcag
     9721 aagctttata acagctacat cggtaatttt gacgattatc tgcagaaggt taatctggcg
     9781 aacacggacc tcggaagcaa ggggcagagc ctggatctga ccaagaaccg tatggcaaat
     9841 cagcagacca cgatggagga attaaagtcc accaacgaag accgggatct gtccgagatc
     9901 attattgact atgcgtcgac gtacactgca tatcaggcat ccttacaggc tgcggcgaag
     9961 atcaatcaga caacattatt gaattatatt taacagacac caaacgggga agctggagga
    10021 gaaccgatga aagcaaacac aagacttttt ggggagattg atattgcgga tgacaagatc
    10081 atttcattgg aggatggaat gatcggactt ccggagtacc ggaattttgc attgatattt
    10141 gatgaggaaa aggaaaaaga caagagttcc gtgatgtggt tccagtccat ggacgatcca
    10201 cagacagcat ttccggtcat gcagccaaac catgtgtgcc cggattacaa cccgatggtg
    10261 agcgatgaga tgctccgtcc gctcggcggg atgacagagg acaatactta cgtgctcgtc
    10321 acacttacgg cagcgccgga tccgaaggat acctcggtga acctcaaggc gccgattgtc
    10381 attaacacag acacccgcag gggagcacag atcattgtgg aagatgacta tcctgtcaaa
    10441 tataagatct atgacgtagt aaaaggaaag aagaaagcgg aggaataagg catgctggca
    10501 ttgacacgca aaaaagggga gtcgctggtc atcaacaata atattgagat cacggtgtta
    10561 gagatccgcg gagatcaggt caagatcgga atcagtgcac cgaaggaagt gccggtatac
    10621 cgcaaggagg tttacctcca gattcagaat gagaacaagg cggcattgaa cgcaacagat
    10681 gcaatggagg cgctgaagaa tcttttctga tgtggttaat aaaagccata gtttctccga
    10741 tataagacat agaaaaccgg gaacatagag gagccatgta tcacacggag gtggtaagag
    10801 atggaaatag agagcatgaa gagcggagcg gcagcgtcgt atcagggaag tgcggcggca
    10861 tctacaaaaa cgctggatgc agcggtacag ccggaagcat ccacacaggc agggctgtcg
    10921 cagacagcag ataaaaagcc cacagtgcag aactttttta cagagaagga tcaggaggat
    10981 aaggatctgt ccgaggaaaa agatgcaaag cagggaacac agttaaaaaa agcggtcaat
    11041 gatctgaaca aacagatgaa gaactcggag gcgatcttcg gaattcacga caagacgaac
    11101 cgtgttacaa tcaagatcat cgataagacc acaaaggaag tcatcaagga gtatccgccg
    11161 gaggagacac tggacatgat cgccaaggta tgggagatcg cgggaatcct tgtggatgag
    11221 aagttataga aaggaacggt gagcatatgc caattcgtat taccggatta aattcaggac
    11281 tggacacaga ggcgatcatc tcggctctgg tatcgtcgta taactataag accaacaagt
    11341 acaagaaagc acagaccaag ctttcctgga agcaggctgc gtggaagacg ctcaatacca
    11401 agatttacag tctgtattcc agcgtgggta acttaaagct ttccagtgca tataatttga
    11461 aatccactac ggtgtcc
//
LOCUS       DBMDQJ010000003        10162 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3039_length_10162_cov_1.611072, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000003 DBMDQJ010000000
VERSION     DBMDQJ010000003.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 10162)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 10162)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10162
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3039_length_10162_cov_1.611072"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..1901
                     /gene="glgB"
                     /locus_tag="ACZO9R_00110"
                     /pseudo
     CDS             1..1901
                     /gene="glgB"
                     /locus_tag="ACZO9R_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521831.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
     gene            2069..5278
                     /locus_tag="ACZO9R_00115"
     CDS             2069..5278
                     /locus_tag="ACZO9R_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6240 domain-containing protein"
                     /protein_id="HIR0911506.1"
                     /translation="MRVEQQSQNIIDVVKQSIGPEEKSGAVLSQYTTGAAGAAVEKSS
                     VSDNAKSVNLKDATYAKPAAEDKQTVAEEIEASATLDAEERKNQMAVLSNTTSPEDYA
                     KMQEDGFSLDDTTTNTIVTETDKIKAQLAKAGVDISFFGDDLDYEQMAAITGSEALAR
                     QLAQAMKEADLPCTEENVRGVMEALSLAQTLQTPGDGAVKYMLDNGMEPTIGNLYKAE
                     YSGSAAYQANPEAAVDMTMFSDQIDAIIARAGLSVDDTSKEACQWLLDNSVPLNEENL
                     AYYMELKNLSLPVQAEALLGSMAAAVAEGKSPQDALLVDGQSFADKAQDACDVIGEAT
                     DEDVAYVVDHGMELNIRSLKEAMAHRSGEETADSASGDAQEETAESSADGSTAYTQRE
                     LDLLTARRQLEETRLAMTAQANYALLKKGIQIDTQPLAELVEALKETENQYYEQLLKG
                     QGVEASETNVNLFRETTERVNDLKTVPAYVLGAQGVKDASVAEVHDTGRAMRDTFERA
                     NQSYETLMTAPRADLGDSIQKAFRNVDDILNDIGLEATEANQRAVRILGYNQIEITQE
                     SVAEMKAADEQVQRVFRNLTPATVTEMIRRGIDPLDMDFSTLNATAEQIKKETGANDE
                     RGFGEFLWKLERTEGISQEERASYIGTYRLIHQVEQSDGAAVGALVNQGADITMRNLM
                     MAVRSERRSGKMDYSVDKSFGKVEGSGYSGTSITDQMEAAYQNNCVKDVADLLTPERM
                     KVVASQTPDWEEMTPEQLKEALAQAQTDDARKDYAYAKEQLDQLSQSAKMTQDIYTVL
                     QKYDIPNTMTNVMAMEAMVNDRNGVFRQIFGESAKGSHKEENEEQLARAKEQVLEDFG
                     EAIASPEGLAAAQEQLAEVAENVMKGMIDSDDVTSLDIREMRLLSAQLSIGSMMAKEE
                     QYAIPVQTESGVVGISLKVVRGDGEKGLVDITMETKLHGKIAATFQAKEHGVSGLIAL
                     DREDTKELLDSRQESLTAVLDSGNEADLHYACIADLDLNHFSTGVFGVDAPEQQETTE
                     KQSDTTYQVQTTRLYHIAESFVRQVQDLLQGTDAQGADA"
     gene            complement(5284..5979)
                     /locus_tag="ACZO9R_00120"
     CDS             complement(5284..5979)
                     /locus_tag="ACZO9R_00120"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR0911507.1"
                     /translation="MKKKLELIMSLCLLAAAFILAKQGAVLVQSEKAAREKICIVVDA
                     GHGGSDPGKVGINGALEKDINLSIALKLKDLLEQREISVVLTRDSDAGLSPADATNKK
                     AADMQKRCQMITDANPAFTVSIHQNSYTTEDIKGAQVFYYGQSENGKRLADVLQEHLI
                     SEVDPQNTRVAKANESYYLLKKTPTPTVIVECGFLSNQSEADLLLTEDYQSKLAHAIY
                     LGILSYLEEESLL"
     gene            complement(6045..7814)
                     /locus_tag="ACZO9R_00125"
     CDS             complement(6045..7814)
                     /locus_tag="ACZO9R_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HIR0911508.1"
                     /translation="MKNTKKNIIPVIAVLVLIVIIILFMLLGRLIEKYTPSKEHQELS
                     EYYGLASDDSVALIFNNEVLSVQGRLIDGNVYLDFETVHDKINSRFFWDANENKLLYT
                     TATDLISADAESTTYYVTKDARTLDHTIVKADASTAYIAIDFVKQYSDFDYTVYDQPN
                     RVVLTNQWGDYNTAPVKKSTELRYQGGIKSPILADLAKGTVLTVLEPDETWTKVMTED
                     GIIGYVKTKALGSTSTATRTSDYTAEEFSHIKKDFSINLGWHQVTNSSANANIANVLS
                     NTKGLNVISPTWFYLKDTNGGIGSLASSDYVNYCHQNNIEVWALVSNFGAKDQGLESP
                     DTTQVLTYSSRRESLINNLISAAIQYKLDGINVDFESLDPSIGDSYIQFIRELSLKCA
                     NNGIVLSVDNYPPTAYTAFYNRSEQAVFADYVILMGYDEHYAGSEEAGSVASIGFVNQ
                     GVADTLQSVPADQLILGMPFYTRVWSETPVSDDGAAVSTTEDTSDSDTLYELDCYSAG
                     MKEVQNLISTNGAVPVWSDTDGQYYVEYINGGVTYKIWVEDATSLEEKLKVMQSNQLA
                     GGAFWKLGLEDPAVWDTIIKYIN"
     gene            complement(8056..8271)
                     /locus_tag="ACZO9R_00130"
     CDS             complement(8056..8271)
                     /locus_tag="ACZO9R_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911509.1"
                     /translation="MNDEHMQRLYLELTSLENRGITIWLEGAKSSSQDVASQLCVQEE
                     STYMRDYIFDEGVLKEVHFDKIRKDLP"
     gene            complement(8379..9092)
                     /locus_tag="ACZO9R_00135"
     CDS             complement(8379..9092)
                     /locus_tag="ACZO9R_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911510.1"
                     /translation="MFRPSYRRKEGDGIFYFDRNETKAHITIGILGCHPGAGVTQLAI
                     SLSTYCASRRRLRTACLELHKRSELARLIPESALADSIHRHALQCSKAPDAADTSKAE
                     FFHFRLHGVDYYPNLAPDAVPRLLNMGYDYLILDLGSLNESAAAEFLRCDRKIVLGSL
                     ALWKTWSYEEFFKQFNNFTNLGEGYHYLVQVGTFQNHSVFSRQHSISMQEVPFIKNPF
                     HIEKEHFLFFDILLSFYSL"
     gene            complement(9040..>10162)
                     /locus_tag="ACZO9R_00140"
     CDS             complement(9040..>10162)
                     /locus_tag="ACZO9R_00140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR0911511.1"
                     /translation="IYDIDEDDTYLYMIEEYIQGESLETFVSHQKSISEELIVKLGIQ
                     LCDIFTYLHHLSPYPILYQDLKPEHIILCGDQVKLLDFGIASFFTGFGKNFQLYGTSR
                     YCAPEILRGQPVTPAADIYSLGRVLTELSDAMSCRCSRQLRCILKQACAADPADRCQE
                     ACDLKAALLRVPLSENQQSSHLIRNIAVIGSRPGAGATHISISLVSTMNQKHIPAIYI
                     AADGSASLLHAARANHRLIDQSGIFIYGSFRGIPDYGDSISVSVPPDDCVVRDYGTRA
                     PALAELASFDLILFVLNGGDWDFVQAAAYGKQLALLPQTRFLCNHGCRSAAKAYARQL
                     GHRVYCFPEDAVPYDTTPEKERLVSSILPKKGGRRHLLF"
BASE COUNT         2618 a         2297 c         2895 g         2352 t
ORIGIN      
        1 tggcaaattt ttcagagctg gatcggtact tatttgggca ggcgacacac tatgagattt
       61 accggaagct gggggcgcat cccacagatg aatacggcat ccggggagtc tgctttgatc
      121 tgtgggcgcc aaacgccgca cgtgtatggg tcatcggttc ctttaacgga tgggatgagg
      181 gcgccaatga aatgaagcgc ctggagccgg agacgatggg gatttttgaa ttgtttattc
      241 cgggtgtcga ggagggtgat ctgtacaagt acctcatcga gacaaaggac ggaaagcgcc
      301 tgtacaaggc agatccttat gcaaactacg cggagttaag acccggaacg gcgtcggctg
      361 ttgcggatat cgaacatttt aagtggtcag atgccgagtg gatgaaagag cgcgccacac
      421 acaaggaaga tgtatatgaa cagccgcttg ccatctatga ggttcacccg ggatcctgga
      481 tgcgtcatcc gggcagacag gatgacggat tttacagtta tcgggatctg gcaaaatctt
      541 tgattccgta tgtgactgag atggggtatt cccatatcga gcttatggga atctgcgaat
      601 atccgtacga tggttcctgg ggctatcagg taaccggtta gtatgcgcct acctcgcgct
      661 atggaacacc ggaagacttc gcttattttg taaatgaatg ccacagacac aagatcggcg
      721 tcatcttaga ctgggttccg gcacattttc caaaggatgc gcacggtctg gcagagtttg
      781 acggaagctg tctctatgag tatgcagacc cgagaaaagg tgagcatccg gactggggca
      841 ctaagatttt tgattacggt aagaacgagg tcaagaattt cctgatcggc agcgccctgc
      901 tgtgggtgga gcattaccat gtggacggtc tgcgtgtaga cgcggtggcg tccatgctgt
      961 atcttgatta cggaaaggag gccggtcagt gggttccgaa ccagtacggc gacaataaga
     1021 acctggaggc aatcgagttc tttaaacata tcaataccct ggttctcgga agaaatccgg
     1081 gagctgtcat gattgcggag gaatctaccg catggccgaa ggtgaccgga ccggtggagg
     1141 atgacggact gaatttcagc tataagtgga acatgggctg gatgcatgat tttttggatt
     1201 acatgaagct ggatccttac tttcgacggg ataatcatca caagatgaca tttgcgatga
     1261 gctacaatga gagcgaaaaa tacattctgg tgctctccca cgatgaggtt gtacacctga
     1321 aatgctccat gattaacaaa atgccgggac ttggcattga taagttccgc aatctcatgg
     1381 caggctacgc gtttatgatg ggacatccgg ggaagaagct tttatttatg ggacaggaat
     1441 ttgcacagct tcgcgagtgg agtgaggagc gtgagctgga ttggttcctt ctcgacaatc
     1501 cggatcacaa gcatatccag aactgggtga aaaaactgct tcatatttac cggaagaatc
     1561 cagcgcttta cgaactcgac agcagctggg gcggcttcga gtggatcaat gcgaacgatg
     1621 cggacagaag catctacagt tttatccgca agagcaaaga cggaaaaaat cagctgctct
     1681 ttgtgtgcag ctttacaccg gtggcaagac cggattacag agtgggagtg cctgccaaga
     1741 agacctacaa gctgatattg gacagtgact ccgaagagtt tggaggatgc ggcagacagc
     1801 gtccgcagag ttacaaggcg gtggaggccg aatgtgacgg cagaccatac tcgatcgcgt
     1861 atcctctgga gccgtacggc gtggcggtat ttcgttactg attgcatttc gggggctggt
     1921 gaaatgcgaa gaacagctta ataatggtgc tgatatgacc gatatatata tcagctatga
     1981 atggcgcata gtgttatgcg ccatttttat gcttcatatt cgattactct atagaaccac
     2041 agctgaaaag gtaaaaggag tgcacaggat gagagtagag cagcagagcc agaatattat
     2101 agatgttgta aagcagagta tcggaccgga ggaaaagagc ggggccgttc tgagccaata
     2161 taccacagga gccgcgggag ctgccgtgga aaaaagcagt gtcagtgaca atgcgaaatc
     2221 cgtgaattta aaagatgcca cctatgccaa gccggcagcg gaagacaagc agacggttgc
     2281 cgaggagatc gaagcgtccg cgacgcttga tgccgaagag cgcaagaacc agatggcggt
     2341 gttgtccaac actacctccc cggaagatta tgcgaaaatg caggaagacg gattttcact
     2401 ggacgatacg acgaccaata cgattgtgac cgagacggat aagattaagg cacagcttgc
     2461 aaaagcggga gtggatatct ctttctttgg agatgatctg gattacgagc agatggcggc
     2521 gattaccgga agcgaagcgc ttgcaagaca gctggcgcag gcaatgaagg aagccgacct
     2581 cccgtgcacc gaggaaaatg tgcggggcgt gatggaggcg ctctcactgg cgcagacact
     2641 tcagacgccg ggggacggcg cagtgaagta tatgctggat aacgggatgg agcccacgat
     2701 cggcaacctg tacaaggcag agtacagcgg aagtgctgct tatcaggcaa atccggaggc
     2761 agcagtcgat atgacgatgt tttcagatca gattgacgcg attattgcgc gcgccgggct
     2821 ttcggtggat gatacatcaa aagaggcgtg ccagtggctg ctggacaata gcgtgccact
     2881 caatgaggag aatcttgctt actatatgga actgaaaaat ctcagtctgc cggtgcaggc
     2941 agaggcgctg ctgggcagta tggcggctgc ggtcgcagaa ggaaaaagcc cgcaggacgc
     3001 actgcttgtg gatggacaat catttgcgga caaggcgcag gatgcctgcg atgtgatcgg
     3061 cgaagcgacg gatgaggatg tcgcatatgt cgtggatcac ggcatggagt taaatatccg
     3121 cagcttaaaa gaagcgatgg cacatcgctc gggtgaggag acggcggata gcgcttcggg
     3181 agacgcgcag gaggagacgg cagagagcag tgcggatggc agcactgctt atacacagag
     3241 agaactggat ctgctgaccg ccagaagaca gctggaggag acacgtcttg cgatgacagc
     3301 acaggcaaac tatgcgttgc tcaaaaaggg aatccagatt gatacccagc cgctggcaga
     3361 gcttgtggag gctttgaagg agacggagaa ccaatattac gaacagctgt tgaagggaca
     3421 gggtgtagaa gcttcggaga cgaacgtgaa tcttttccgt gagaccacag agcgtgtcaa
     3481 cgatttaaaa acggtaccgg catacgtgct gggagcgcag ggagtaaagg atgcgtctgt
     3541 cgcagaggtt catgataccg gacgtgcgat gcgggatacc tttgagcgtg caaatcagag
     3601 ttatgagacc ctgatgacgg caccacgtgc agacctgggg gattcgattc agaaggcatt
     3661 ccgcaatgtg gatgatattt tgaacgatat cggactggag gcgacggagg caaatcagcg
     3721 tgcagtgcgc atcctcggtt ataaccagat tgaaattaca caggaatccg tggcggagat
     3781 gaaagcggcg gatgagcagg tgcagcgtgt attccgcaac ctgacgccgg caacggtgac
     3841 ggagatgatc cgcagaggaa tcgatccgct ggatatggat tttagtacgc tgaatgcgac
     3901 agcggagcag ataaaaaagg agactggcgc gaacgatgag cgcggcttcg gagaattttt
     3961 gtggaagctg gaacgcacgg agggaatcag ccaggaagag cgggcttctt atatcggaac
     4021 gtatcgcctg atccatcagg tagagcagtc ggacggcgcc gcggtgggag cgcttgtaaa
     4081 tcagggagcc gatatcacca tgcgcaatct gatgatggcg gtccgatccg agaggcgcag
     4141 tggaaaaatg gattacagtg tggataaatc ctttggaaaa gtggaaggca gtggatattc
     4201 cggcacttcc atcaccgatc agatggaagc ggcatatcag aacaactgcg tgaaagatgt
     4261 cgcagatctg ctcacaccgg agcgcatgaa ggttgtcgca tcacagacgc cggactggga
     4321 ggagatgact ccggagcagt taaaggaggc gctcgcgcag gcacagacag acgatgccag
     4381 aaaggattat gcatatgcga aggagcagct ggatcagtta tcgcagagcg cgaaaatgac
     4441 gcaggatatt tacacggtac tgcagaagta cgacattccg aataccatga cgaacgtcat
     4501 ggcgatggaa gccatggtaa atgaccggaa cggcgtattt cgtcagatct tcggcgagag
     4561 cgcaaaggga agccacaaag aggaaaatga ggagcagctt gcccgggcga aggagcaggt
     4621 gcttgaggat ttcggagagg caatcgcaag cccggaggga cttgccgctg cacaggaaca
     4681 gcttgctgag gttgctgaga atgtaatgaa gggaatgatc gacagcgatg acgtgacaag
     4741 tcttgatatc agggagatgc gtctgttgtc ggcacagtta tccatcggca gcatgatggc
     4801 aaaagaagag cagtatgcaa taccggtgca gaccgagagc ggtgttgtcg gcatttcgct
     4861 gaaggtcgtg cgtggggatg gtgaaaaagg gctggttgac atcacgatgg aaaccaagct
     4921 tcacggtaag attgcggcaa cgttccaggc aaaagagcat ggcgtcagcg gtctgattgc
     4981 attggaccgg gaggatacca aagaactttt agacagccgg caggagagcc ttacggcagt
     5041 attggacagt ggaaacgagg cagatcttca ctatgcatgc atcgcagatc tggatctgaa
     5101 ccatttttca acaggcgtct ttggcgtgga tgcaccagaa cagcaggaga ccacagagaa
     5161 acagtcggat acgacgtatc aggtgcagac aacaaggcta taccatattg cggaaagctt
     5221 tgtgcggcag gtgcaggatc ttttgcaggg aacagatgcg cagggagcgg atgcataaaa
     5281 cagttaaagc agggattctt cttcgagata ggagagaatc cccagataga tggcatgagc
     5341 aagcttgctc tgataatctt cggtcaggag cagatcggct tcgctctggt tgcttaaaaa
     5401 cccacattct acgattaccg taggtgtggg tgtttttttt aggagatagt agctttcgtt
     5461 tgccttggcg acgcgtgtat tctggggatc gacttcggaa atcagatgct cctggagaac
     5521 gtccgctaag cgcttgccgt tttcggactg cccgtaatag aatacctggg cgcctttgat
     5581 gtcttcggtt gtgtaactgt tctgatgaat gctcacggtg aaagcgggat tggcgtccgt
     5641 gatcatctgg cagcgcttct gcatatccgc ggcttttttg ttggtggcgt ctgcgggtga
     5701 gagaccggcg tcggaatcac gggtcagaac gacagagatt tctctctgtt ccagaagatc
     5761 ttttaatttt aaagcgatgg agaggttgat gtccttttcg agtgcgccgt tgatgcccac
     5821 ctttccgggg tcacttccgc cgtgaccggc atcgacgaca atacagattt tttcacgtgc
     5881 cgccttttca ctctggacca gtacggcgcc ctgctttgcg agaatgaagg cagcggccag
     5941 aagacagagg ctcatgataa gttccaactt ttttttcatg aaataaaaaa cccccgggga
     6001 ttcgttcata aaatgatatt cacgatcccc gggggttaga acggctagtt gatgtatttg
     6061 ataatggtat cccagaccgc cggatcctcc agaccaagct tccagaaagc tccgccggcg
     6121 agctggttgg actgcattac ctttaacttc tcctcaagtg aagtggcatc ctccacccag
     6181 atcttgtagg tgacgccgcc gttgatgtat tctacataat actgtccgtc tgtgtcggac
     6241 cacaccggaa cggctccgtt cgtgctgatc aggttctgga cttctttcat gcctgccgag
     6301 tagcagtcca gctcgtagag tgtatcactg tcggaggtgt cctcggttgt actgacagcc
     6361 gcgccatcgt ccgataccgg agtctcgctc cagacacgtg tgtaaaacgg cataccgaga
     6421 atcagctggt ctgccggaac gctttggagc gtatctgcga caccctgatt gacaaaaccg
     6481 atggacgcca ccgatccggc ttcctcggaa cctgcgtaat gctcatcata tcccatcagt
     6541 atgacatagt cggcaaagac tgcctgctcg gagcggttat agaatgccgt gtaagctgtt
     6601 ggcggataat tatcgacgga aaggacaatg ccgttgttgg cacatttcag cgataattcc
     6661 cggataaact ggatatagga atcaccgatg gaagggtcga gcgattcaaa gtccacgtta
     6721 atgccgtcca gcttgtactg gatcgcagcg gagatcaggt tgttgatcag actctcacgt
     6781 ctggaactgt aggtcagcac ctgcgtggta tccggactct ccagtccctg atccttggcg
     6841 ccgaagttgc ttacaagcgc ccagacttca atattgttct ggtgacaata gtttacataa
     6901 tcagaacttg ccagggaacc gatgcctccg ttcgtatcct ttaagtaaaa ccaggtcgga
     6961 gagatgacat tgagtccctt ggtgtttgaa agtacgttcg caatgtttgc gttcgcactg
     7021 ctgttggtta cctggtgcca gccgagatta atggagaaat cttttttaat atgcgaaaat
     7081 tcttccgcag tgtagtcgct ggtccgggtc gcggtggaag tgctgccgag ggctttggtc
     7141 tttacatagc cgatgatgcc gtcctccgtc atcaccttcg tccaggtctc gtccggctct
     7201 aaaaccgtga gcacggttcc ttttgcaagg tcggcgagaa tcggactctt gatgccgccc
     7261 tgatagcgca gctccgtgga tttcttgaca ggagcggtgt tgtagtcgcc ccactggttt
     7321 gtgaggacaa cgcggttcgg ctgatcgtat acggtataat caaaatcgga atactgtttt
     7381 acaaaatcga tcgcaatata tgcggtggag gcatctgcct ttacaatcgt gtgatctaac
     7441 gtccgggcgt ctttggtcac atagtaggtc gtgctctccg catccgccga gatcaggtcg
     7501 gtggcggtgg tgtataaaag cttgttctca ttggcatccc agaaaaaacg gctgttgatc
     7561 ttatcgtgaa ccgtctcaaa atccagatat acgtttccgt cgatcagtcg cccctgcaca
     7621 gacaacactt cattattaaa gatcagggct acggaatcat cagatgcaag cccgtaatac
     7681 tcggataact cctggtgctc tttggagggc gtgtattttt cgatcagtcg tccgagcagc
     7741 atgaacagaa tgataatgac gattaaaaca agaacggcaa tgaccggtat aatattcttt
     7801 tttgtattct tcatggaaaa atcctttctt cttctgagag atttccccta cattgtagca
     7861 tattgcagca caaaatcaaa gctatttgca tctgccattt tttgcgtgcc gcagaatgaa
     7921 aaacgggaac cggccgcggg agattgtgaa ctcgggcggc agttcccggc tgtcttttta
     7981 tcaaataata ttcactgcat agacagtatg accgggcatt gaggaactca gtcattctgt
     8041 tccagttacg actagttacg gaagatcttt tctgatctta tcaaaatgga cttcctttaa
     8101 caccccctcg tcaaaaatat aatctctcat gtacgtgctt tcctcctgca cgcagagctg
     8161 gctggctaca tcctgcgagc tgcttttcgc gccctccagc cagatggtaa tgcctcgatt
     8221 ctccagagac gtaagctcca gatagagtct ttgcatatgt tcatcattca cattagattc
     8281 ccccaaatct gaaaaatacc aaaaggaatt cgctggttac gtttcgtgat ataaagaaaa
     8341 attgcgctgc ctgatccccg gcagacagtg tgttgcggtt acaatgaata aaatgacaga
     8401 agtatgtcaa aaaaaagaaa gtgctccttc tctatatgaa aagggttttt gatgaacgga
     8461 acctcctgca tggatatgga gtgctgcctg gaaaaaacgg aatgattctg gaatgttccc
     8521 acctgtacca gataatgata accttcccca aggtttgtaa agttgttaaa ttgtttgaaa
     8581 aattcctcat aactccaggt tttccagagt gcaagactgc ctagcacaat cttgcggtca
     8641 cagcgcagga attctgcggc agcggattcg tttaagctgc cgagatccag gatcagataa
     8701 tcatacccca tattcagcag gcggggcaca gcatccggtg ccagattcgg ataataatcc
     8761 acgccgtgca ggcggaagtg aaaaaactct gcctttgaag tgtccgcggc atccggcgct
     8821 ttgctgcatt gtaaggcatg gcggtgtatg gagtccgcaa gagcagattc cggaatcagc
     8881 cgcgccagtt cgctgcgctt gtgaagttca agacaagcgg tgcgcagtct tcgcctggag
     8941 gcacagtatg tgctaaggct tatggcgagc tgtgtcacgc cagcccccgg atgacatccg
     9001 agaatgccga tggtgatgtg tgctttggtt tcgttgcgat caaaatagaa gatgccgtct
     9061 ccctcctttc ttcggtaaga tggacgaaac aagccgttcc ttttcgggtg ttgtatcgta
     9121 tggaaccgca tcttccggaa agcagtagac gcggtggcct aactgccgtg cgtatgcctt
     9181 tgcggcagag cggcagccgt gattgcagag aaaacgtgtc tgtggcagca gtgccagctg
     9241 tttcccatag gcggctgcct gtacaaaatc ccagtctcct ccgtttaaga cgaaaagaat
     9301 caggtcaaaa gaagccagct cggcaagcgc gggcgcgcgg gtgccatagt cccttacaac
     9361 gcagtcatcc ggcggaacgg aaacagatat actgtcccca tagtccggaa tcccgcggaa
     9421 gcttccataa ataaagatcc cgctctggtc gatcagacga tgatttgccc tggcggcgtg
     9481 tagaagcgat gcgctcccgt ccgcggcaat atagattgcg ggaatatgct tttgattcat
     9541 ggtgctgaca agagaaatgg aaatatgcgt ggcgcctgcc ccgggtctgc tcccgatgac
     9601 ggcaatgttc cgaatgagat gcgaagactg ctgattctct gataatggta cacgaagcag
     9661 tgcggctttc agatcgcagg cctcctgaca gcggtctgcc ggatctgcgg cgcacgcctg
     9721 cttcaagatg caccgaagct gtctggaaca gcggcaggac atggcgtccg aaagctccgt
     9781 taaaaccctg ccgagactgt agatgtcggc agccggagtg acgggctgtc cgcgcaggat
     9841 ttccggtgcg cagtagcggc ttgtcccgta aagttggaaa ttttttccga aaccggtaaa
     9901 aaaagatgca atgccaaaat cgagtaattt tacctgatct ccacataata taatatgctc
     9961 cggttttaag tcctgataca ggatcgggta tggagataaa tgatgcagat aggtaaaaat
    10021 gtcgcatagc tgtatgccca gttttacgat cagttcctca gaaatgcttt tttgatgaga
    10081 gacaaacgtc tcgagtgatt caccctgaat gtattcttca atcatataaa gataggtgtc
    10141 atcttcgtcg atatcatata tg
//
LOCUS       DBMDQJ010000004         9714 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3181_length_9714_cov_1.715906, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000004 DBMDQJ010000000
VERSION     DBMDQJ010000004.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3181_length_9714_cov_1.715906"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="ACZO9R_00145"
     CDS             <1..481
                     /locus_tag="ACZO9R_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR0911512.1"
                     /translation="EAGLHPVRFLLNYPGYVEGWATYVEMISYHYAGLDDDLASLLSL
                     NQSALLSLYASTDLGIHYDGWSFSDTTAFWKDFGITDQTALREIYELIVEEPAHYLKY
                     YVGYMEFVDLKEKAQETYGSAYSDIAFHKALLSIGPAPFSIIETYLDDYYLPDAAPQ"
     gene            complement(494..952)
                     /locus_tag="ACZO9R_00150"
     CDS             complement(494..952)
                     /locus_tag="ACZO9R_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HIR0911513.1"
                     /translation="MPKTKFQNVIFTLLMAFAMVYAMICYNIAINRGGMTNEVFMLAF
                     HEVIIMWPVAFVLEMFVVERLAKMLAFRLVSPDDKPIFILLAISSMIVCLMCPMMSLV
                     ATILFQNPGKEIIALWFQTTVLNFPMALCWQIFFAGPLVRLIFRTVSRAK"
     gene            complement(1156..1995)
                     /gene="rsmI"
                     /locus_tag="ACZO9R_00155"
     CDS             complement(1156..1995)
                     /gene="rsmI"
                     /locus_tag="ACZO9R_00155"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR0911514.1"
                     /translation="MAGKLYLCATPIGNLEDITYRVVRTLKEVDLIAAEDTRNSIKLL
                     NHFEIRTPMTSYHEYNKIEKAYQLVDKMREGLNIALITDAGTPGISDPGEDLVRICYE
                     EGIEVTSLPGPAACITALTMSGQPTRRFAFEAFLPKEKKERAAVLSQLVNETRTIIIY
                     EAPHHLIRTLEELYETLGDRKLTVCRELTKRYEEKTLTTFSEILQYYKDNEPRGEYVL
                     IIAGKTFEELKQEEQKNWENLSLEEHMEVYEKQGIGRKEAMKLVAKDRGISKRDVYQA
                     LLS"
     gene            complement(2062..2841)
                     /locus_tag="ACZO9R_00160"
     CDS             complement(2062..2841)
                     /locus_tag="ACZO9R_00160"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR0911515.1"
                     /translation="MFNDIVIGPITIHMYGVMIAAGFLAALLMTLRRGKKRGYDEDLI
                     WGIFFCAIIGGMLGTRILYYIVEIPEIMKDPSILWDFKNGYVVYGGIIGGILASYIYC
                     RTKKQAFMPYFDLVMPAVSFAQGFGRFGCFFAGCCYGRETDAWYGITFHNSSFAPNGV
                     KLIPTQLISAAGDFIICALLLWFASKRPKTGRVAAAYLVLYGIGRFAVEFLRNDYRGS
                     VGVLSTSQLISIGAVIGGIVLYFAAPKLAGVGNVAEEKSVG"
     gene            complement(2907..3650)
                     /locus_tag="ACZO9R_00165"
     CDS             complement(2907..3650)
                     /locus_tag="ACZO9R_00165"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR0911516.1"
                     /translation="MTPNNLVHENERLDELHRNGYYIIQDPARFCFGMDAVLLSGFAR
                     ARRGERVLDLGTGTGIIPILMEAKTEAEDFTALEIQEESADMARRSVLYNHLEEKIRV
                     VTGDIKDASGIFGASSFDIVTTNPPYMIGQHGISSGAEAKAIARHEVLCTLDDILRES
                     AKLLVPGGRFYMVHRPFRLAEIMSKMVAYGIEPKRMQLVYPYVDKEPNMVLLEGSRGG
                     RSRLTVEKPLIVYKEQGVYTDEIYDIYGY"
     gene            complement(3637..4548)
                     /locus_tag="ACZO9R_00170"
     CDS             complement(3637..4548)
                     /locus_tag="ACZO9R_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PSP1 domain-containing protein"
                     /protein_id="HIR0911517.1"
                     /translation="MVKVVGVRFRNAGKIYYFGPGKLDIHAGMHVIVETARGVEMGTV
                     MTEPKGVSEEEVIQPLKPVIRIATEADEKTVAKNREKEKDAFRICLEKIAKHKLDMKL
                     VEAEYTFDNNKLLFYFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDETKIMGGIGI
                     CGRALCCNTYLSEFAPVSIKMAKEQNLSLNPTKISGVCGRLMCCLKNEEETYEYLNSR
                     LPGIGDRVTTVDGLKGEVSSVNVLRQMVKVLVEVDDEKELREYPVDELKFRPRRRKEN
                     IKLSAKELKELSALEDKGGKSKIDDTK"
     gene            complement(4553..5575)
                     /locus_tag="ACZO9R_00175"
     CDS             complement(4553..5575)
                     /locus_tag="ACZO9R_00175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR0911518.1"
                     /translation="MAGFKAILGHEQIIEHLQNAVTMDKVSHAYIINGPDKSGKMMLA
                     EAFAQTLECEKLEDVVENAAQPSDVEPCMECHSCKQAMTKNQPDIIYVRHEKPNTISV
                     DDIRTQVNNDIVIKPYSSRYKIYMIDEAEKMNEQAQNALLKTIEEPPAYAILILLTTN
                     AAAFLPTILSRCVTLNIKAVPDEKIRGYLMRKYQIPDYQADVCVAFAQGNVGKAIQLA
                     SSEEFNELKDAVLQLIKRLRDIDLYEMTEAVKQIATYKLSINDYFDLMMVWYRDVLLF
                     KATGDVNGLIFKDEVYDIKRQAEKSSYSGIETILEALRKAQIRLNANVNFDLTMELLL
                     LTIKEN"
     gene            complement(5594..6034)
                     /locus_tag="ACZO9R_00180"
     CDS             complement(5594..6034)
                     /locus_tag="ACZO9R_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HIR0911519.1"
                     /translation="MDIKVNQAQMVTQVPDTVTTETGDGTFKFTLASAITDAELQAKV
                     DSLLSDITFQGNRIAEHMDIRDMKKYRGLIRDFMNEVVYRSHKFSRENFLDRKGRHRV
                     YGLIRLIDANLDELAQELVKDEKDHITILSKIGEIQGLLLDILT"
     gene            complement(6060..6650)
                     /locus_tag="ACZO9R_00185"
     CDS             complement(6060..6650)
                     /locus_tag="ACZO9R_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR0911520.1"
                     /translation="MGKIYCMMGKSSTGKDTLYKMLLEDGALSLKKIIPYTTRPMRRG
                     ETDGVEYYFCGEKELADLLKRGKVIELRAYDTVQGVWKYFTVDDHQIQDLRQNYLLIG
                     TLEAYGKLRDYFGKERIVPIYIEVEDGIRLERAIGREQQQREPHYEEMCRRFLADAAD
                     FSEEKLLDAQITVRFANDDLKQTEENIRDYILSQND"
     gene            complement(6680..8134)
                     /locus_tag="ACZO9R_00190"
     CDS             complement(6680..8134)
                     /locus_tag="ACZO9R_00190"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0911521.1"
                     /translation="MYQQLISYGESDVYPFHMPGHKRRALPFPNPYTIDITEIDGFDN
                     LHHAEGLIREAEERAAKLYGADRSYYLVNGSTCGLLAAICAAARRGDKVLAARNCHKA
                     VYHAISMQGLAAEFLYPAITRGDLQGQITAAQVEEALTKHPDIAVVILTSPTYEGIVS
                     DVAAIAACCHAHGAALIVDEAHGAHFGFGAGFPENAVRLGADAVIMSLHKTLPSFTQT
                     ALLHCNGTRIDPGRVARYLGVYETSSPSYLFMAGMDACIDLIREQGAELFAEYRRRLD
                     AFYRDTADLAQLHVMRREDLCKEEAYDWDDSKLIIYAGAMGGEALHQELLGHYHLQME
                     MLSADYVLGMTSLMDTDEGMRRLVTALHEIDEKNRRMAEPHETAEENRKTELDGEVPE
                     AGFTARMYRKNPRRMQIYQALDLPYREMPLDEAVGKMAADYVYLYPPGIPLIVPGEVI
                     TEEFIRHIRECRERKLNVEGQGNLAPGRIKIVYF"
     gene            8290..8895
                     /gene="mobA"
                     /locus_tag="ACZO9R_00195"
     CDS             8290..8895
                     /gene="mobA"
                     /locus_tag="ACZO9R_00195"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR0911522.1"
                     /translation="MKHALIILCGGGSTRMGTDKALLPFGEVSLLEYLVNKYKPHFSH
                     IYLSVKKPGEYTHLSLPVTEIADLYMNAGPMSGVFSGLSMMDEDQAFILPIDTPFLEP
                     EVGLQLLEHLDGFDIAAVSGTETDQKVPASAYSKSCLPSIGKCLLLHQFTFDTLREKC
                     SVSYLKREEATKDSDIPADLQFSHLDTRDDYYLALRHLHCI"
     gene            complement(8896..>9714)
                     /locus_tag="ACZO9R_00200"
     CDS             complement(8896..>9714)
                     /locus_tag="ACZO9R_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911523.1"
                     /translation="EELMYLIEDSPFSLFLWLKNSGALGVEQYRKKNLLTLDFAYVTD
                     PRAMKREGIVPGQELSLKMGYVMLAYILKVEKVPDISWKGRASAGDHRVDLEIAGEFE
                     EMTVPIHIRVTELTEEGLVPVKRTFMPFMEGGKQIPYLEYPVESILAENLFYLIRDME
                     LLPDMSVYDKVYGILKTEPVDGRHIQELLGEHCKKQQLLPEESRMKEILSYRDYSYMR
                     KRWEKYLRHRKRKEPAWTEVMQVLEQFLPQMWSTLCRDEIFFGDWMPDLQRFLD"
BASE COUNT         2169 a         2898 c         2127 g         2520 t
ORIGIN      
        1 tgaagccgga ctacatcccg tgcggtttct gctgaattat ccgggctatg tcgagggctg
       61 ggcgacctac gtggagatga tctcgtacca ttatgccggg ctggatgatg acctcgcctc
      121 gctgctctcc ctgaatcagt ccgccctcct aagtctctat gcctcaacgg atctgggaat
      181 ccactacgac ggctggagct tttccgacac aaccgctttc tggaaagact tcggcatcac
      241 ggatcagacc gcactgcgcg aaatctatga gctgatcgtg gaagaacccg cccattatct
      301 gaaatactat gtgggatata tggaatttgt ggatttaaaa gaaaaggcac aggaaaccta
      361 cggaagcgcc tacagcgata tcgcttttca taaggctctg ctctccatcg gtcctgcacc
      421 tttttcgatc attgaaacgt atctggatga ttattatctg ccggatgcgg ctccccaata
      481 gccccggcgt aacttatttc gcgcgggata ctgtacggaa aatcaggcgc accagcggtc
      541 ctgcaaaaaa gatctgccag cagagcgcca tcgggaaatt aagaacggtc gtctggaacc
      601 acagcgcaat aatttctttg cccgggttct ggaagagaat ggtcgccaca agactcatca
      661 tcgggcacat aaggcagaca atcatgctgg aaatcgcgag cagaataaaa atcggcttat
      721 catccggtga gacaaggcgg aaggcaagca tctttgcgag gcgttccaca acgaacattt
      781 ccagcacaaa agcaaccggc cacatgataa ttacctcgtg gaacgcgagc atgaaaacct
      841 cgttcgtcat tccgcctcgg ttgattgcga tgttgtagca gatcatggcg tagaccatgg
      901 cgaacgccat gagaagtgtg aagatgacat tttgaaattt ggtttttggc ataagtaact
      961 ctccttttct ctgcaaaata catacgtgtt gttcgttcat caggcgagct tatgcgtcac
     1021 atgtatcgtt tccaattaaa ccaaaatgtt tttgcgaatt gtaatatgat attggtaaaa
     1081 aacgctgcaa cacggtcatc cccgtttgca gcgttttcta atataacaca gtattttcat
     1141 ttatgtaagt attttttaag acaacagcgc ctggtagaca tcgcgcttgc tgatgccgcg
     1201 gtcctttgcc accagcttca tcgcttcctt ccggccgatc ccctgcttct catagacttc
     1261 catatgctcc tcgagagaca ggttctccca gttcttctgc tcttcctgct tcaattcctc
     1321 aaatgtcttt ccggcgatta tcaggacata ctctccgcgg ggttcattgt ctttataata
     1381 ttgaagaatc tccgagaatg tcgttaatgt cttttcctcg taccgctttg tcagttccct
     1441 gcacacggtt aatttgcggt ctccgagcgt ctcatacagc tcttccagcg tgcggatcag
     1501 atgatgcggc gcctcgtaga tgatgatcgt gcgcgtctca ttgaccagct gcgagagcac
     1561 tgctgcccgc tcttttttct ccttcggcag aaacgcctca aacgcaaacc gtctggtcgg
     1621 ctgccccgac atcgtgagtg cggtgataca tgccgccggt cccggcagag atgtcacctc
     1681 aatcccttcc tcatagcaga tgcgcacgag gtcttccccc ggatccgaga tccccggcgt
     1741 ccccgcatcc gtaatcagtg cgatgttgag tccttcgcgc atcttatcca ccagctgata
     1801 cgctttctcg atcttattgt actcgtgata gctcgtcatc ggcgtccgga tctcaaaatg
     1861 attcaacagc ttgatggaat ttctggtatc ctccgccgca atcagatcga cttcctttaa
     1921 ggttctcacc acccggtagg tgatgtcctc cagatttccg atcggagtgg cacacaaata
     1981 taactttccc gccataactt ttcctttcgt cgtgcataaa gcgcgcacat cctgtatact
     2041 tcccgcgtgc attcctgcct gctatccgac agacttctcc tcggccacat tccccactcc
     2101 tgcaagcttc ggtgcagcga aatacagtac gattcctccg atcaccgcgc cgatggagat
     2161 caactgcgac gtggacagca cacccacact gcctctgtaa tcgttgcgca aaaactccac
     2221 ggcaaatctt ccgattccgt acagcacaag ataagccgcc gcaacgcgtc cggtcttcgg
     2281 tctcttcgat gcaaaccata acagcagcgc gcagatgata aaatctcccg ccgctgagat
     2341 cagctgcgtc ggtatcagct tcacaccgtt cggtgcgaac gaggagttat gaaacgtgat
     2401 tccgtaccac gcatccgtct ccctgccgta gcaacagccc gcaaaaaagc agccaaagcg
     2461 gccgaagcct tgtgcaaacg aaaccgccgg catcaccaga tcaaaatacg gcataaacgc
     2521 ctgctttttc gtgcggcagt agatataact tgccagaatt cctccgatga ttcctccgta
     2581 gaccacatag ccgttcttga aatcccagag gatagacgga tccttcatga tctccggaat
     2641 ctctacaata taatataaaa tcctggttcc aagcattccg ccgatgatgg cgcaaaagaa
     2701 aatgccccag atgagatcct catcatagcc tctctttttg ccccggcgca gcgtcatcag
     2761 caatgccgcc agaaaacccg ctgcaatcat cactccatac atatgaatgg taatcggtcc
     2821 tatcacaata tcattaaaca tattcttttc ctttctgcca cagcgtcctg tccgctgctt
     2881 tctgtgccgc catacccggt catttgttaa tatccgtaga tatcatagat ttcatccgtg
     2941 tatacaccct gttccttata aacgatcagg ggtttttcca cggtcagtct cgatctgccg
     3001 ccgcggcttc cctccagcag aaccatgttc ggctccttgt ccacgtacgg atagaccagc
     3061 tgcatccgct tgggttcaat gccgtaagca accattttgc tcataatctc cgcaagccgg
     3121 aacggacggt gtaccatgta aaatctcccg cccggcacaa gcagcttcgc cgactcccgc
     3181 agaatgtcgt ccagagtaca cagaacctca tgacgtgcga tcgctttggc ctccgcgccg
     3241 gagctgattc cgtgctgccc gatcatatac ggcggattgg tggtgacaat atcaaaagaa
     3301 gatgccccga atattcccga ggcatctttg atatcgcctg tcacaacccg gatcttttct
     3361 tccaggtgat tatataatac gcttcttctc gccatatccg cgctctcttc ctgaatctcc
     3421 agcgcggtaa aatcttctgc ctctgtcttt gcctccatta agatcgggat aattccggtg
     3481 cccgtcccca gatcgagcac gcgctctccc cgtctggcac gcgcgaatcc cgacaacagc
     3541 acggcatcca taccgaaaca gaatctcgcc ggatcctgaa taatatagta gccgttccgg
     3601 tgcagttcat ccagacgctc gttttcatgg acaagattat ttggtgtcat caatcttgga
     3661 ttttcctccc ttatcctcaa gcgcggacaa ttccttcagc tcctttgcag aaagctttat
     3721 attttccttt ctgcggcgcg gacggaactt cagctcgtcc accggatatt cccggagttc
     3781 tttttcatcg tccacctcta ccagtacctt caccatctgg cggagcacat tgaccgaaga
     3841 cacttctcct ttcagtccgt ccaccgtcgt cacgcggtcg ccgatacccg gaagtctgct
     3901 gttgagatac tcgtaggtct cttcctcatt cttcaggcag cacatcagtc ttccgcacac
     3961 acccgaaatc ttggtcggat tcaaagaaag attctgctct tttgccatct tgatggaaac
     4021 cggcgcaaat tccgagagat aggtgttgca gcacagcgca cgtccgcaga taccgattcc
     4081 gcccatgatc tttgtctcat cgcgcacacc gatctggcga agctcgattc tggtgcggaa
     4141 taccgctgcg aggtctttta ccagctcacg gaaatcgatt cttccgtccg ccgtgaagta
     4201 aaacaacagc ttattattgt caaacgtgta ttccgcctcg acaagcttca tatcgagctt
     4261 gtgcttggca atcttctcga gacagatgcg gaaggcatcc ttctcttttt cccggttctt
     4321 cgcaaccgtc ttctcgtccg cctccgtcgc aatgcggatg accggcttca acggctggat
     4381 gacttcctcc tccgaaaccc ctttcggctc cgtcatgacc gttcccatct cgacaccgcg
     4441 ggcggtctcc acgattacgt gcattcccgc atggatgtcc agcttgcccg gtccaaaata
     4501 atatatcttg cccgcattcc gaaagcggac tcctactact tttaccatat ttctagttct
     4561 cctttattgt cagcaacagc agttccattg taaggtcaaa gttgacgttg gcattcagcc
     4621 ggatctgtgc tttgcgcagc gcctccaaga tcgtctcgat gccgctgtaa gaactcttct
     4681 ctgcctgccg cttgatgtcg tacacttcat ccttgaagat cagaccgttc acgtcgccgg
     4741 ttgccttaaa cagcagcacg tcccgatacc ataccatcat cagatcaaag taatcattga
     4801 tcgaaagctt gtatgtagca atctgtttta ccgcctctgt catctcatac aaatcgatgt
     4861 cccgaagcct cttgatcagc tggagcaccg cgtcctttaa ctcgttgaac tcctccgagg
     4921 acgcaagctg aatggctttt ccgacgttac cctgagcgaa tgccacacat acgtccgcct
     4981 ggtagtccgg gatctgatac ttgcgcatca gatagccgcg gatcttctca tccggcactg
     5041 ccttgatgtt caatgtgaca cagcgggata agatcgtcgg cagaaaagcg gcagcgtttg
     5101 tggtaagaag aatcagaatc gcgtaggcag gcggttcctc aatcgtctta agcagcgcat
     5161 tctgtgcctg ctcgttcatc ttctcggctt catcgatcat atagatctta tatctgctgc
     5221 tgtaaggttt gattactata tcgttgttga cctgagtacg gatatcatcg accgagattg
     5281 tattcggctt ctcatgccgc acatagatga tgtccggctg gttcttcgtc atcgcctgct
     5341 tacaggaatg acattccata cacggctcca catcggacgg ctgcgcggca ttttccacca
     5401 catcctccag cttctcacac tcgagtgtct gggcaaacgc ctccgcgagc atcattttgc
     5461 cggacttatc cggtccgttt atgatgtatg catgtgatac cttatccatt gtgactgcat
     5521 tctgcagatg ctctatgatc tgctcatgcc ctaaaatagc tttaaatcct gccatagctt
     5581 ccctcgtttt atgttaagtc agaatatcca acagaagtcc ctggatctcc ccgatcttgc
     5641 tcaagatcgt gatgtgatcc ttctcatcct tgaccagttc ctgtgcaagt tcgtccaggt
     5701 tggcatcaat cagccggatc agtccgtaga ctctgtggcg tcccttgcgg tccaggaaat
     5761 tctctcttga aaacttatga gagcggtaga ccacttcatt cataaaatca cggatcagtc
     5821 cgcggtactt tttcatatcg cggatgtcca tatgttccgc aatccggttc ccctgaaacg
     5881 taatgtccga taacagactg tccaccttcg cctgaagctc tgcatccgtg atcgcgctcg
     5941 ccagcgtgaa cttgaacgtt ccgtcgccgg tctccgttgt caccgtgtcc ggcacctgcg
     6001 ttaccatctg tgcctgattc accttaatat ccaatctgcc accctctttc cctgatccgt
     6061 caatcgttct gagacaatat ataatcgcgg atattttctt ccgtctgctt taaatcatca
     6121 tttgcaaacc gcaccgtaat ctgtgcgtcc aaaagctttt cttccgaaaa atctgccgcg
     6181 tccgccagaa atctccggca catctcttca tagtgcggtt cccgctgctg ctgctctctt
     6241 ccgatcgcac gctccagccg gattccgtcc tccacttcaa tatatatcgg aacaatacgt
     6301 tcctttccga agtagtcacg gagcttcccg tatgcttcca gtgtaccgat cagcagataa
     6361 ttctgccgca gatcctgtat ctgatggtca tccaccgtaa aatacttcca tactccctgc
     6421 accgtatcgt atgcccgaag ttcaatgact ttgccacgct ttagaagatc ggccagttcc
     6481 ttctctccgc agaaataata ttccacgccg tccgtttctc cccggcgcat cggccgcgtc
     6541 gtgtagggaa tgatcttctt cagcgaaagc gccccgtctt ccagaagcat cttatataat
     6601 gtatcttttc ctgttgagct cttgcccatc atgcaataga tttttcccat tcgctgcacc
     6661 ttctagtata atatagtctt taaaaatata caatctttat ccgaccgggg gcaagatttc
     6721 cctgcccttc cacgttcagt tttctctccc ggcactctct gatgtgccgg ataaactctt
     6781 ccgtgataac ttctcccggc acgatgagcg ggattccggg cggatacagg tagacgtaat
     6841 ccgccgccat tttcccgact gcctcatcaa gcggcatctc ccggtacggc agatccagcg
     6901 cctgataaat ctgcatccgg cgcggattct tccggtacat gcgcgccgtg aatccggctt
     6961 ccggcacttc tccgtctaat tccgtcttcc ggttctcttc agcggtctca tggggctccg
     7021 ccatacgccg gttcttttca tcgatctcat ggagcgccgt cacaagccgc cgcatgccct
     7081 cgtccgtatc cataaggctt gtcatgccga gcacatagtc cgccgacagc atctccatct
     7141 ggagatgata gtgtccgagc agttcctgat gcagcgcctc gccgcccatg gcgcccgcat
     7201 agataatcag cttggaatcg tcccagtcat acgcttcttc cttgcagagg tcttcccgcc
     7261 gcatgacatg cagctgcgca agatccgccg tatcacggta aaatgcatcc agacgtctcc
     7321 ggtattccgc aaaaagttct gccccctgct cacggatcag gtcgatgcat gcatccattc
     7381 ccgccataaa cagatacgac ggcgaactcg tctcatagac gcccagatac cgtgccacgc
     7441 gcccgggatc aatccgcgtg ccgttacaat gcaaaagcgc cgtctgtgta aacgacggca
     7501 gcgtcttgtg cagactcata atcacggcgt ccgccccaag gcgcaccgca ttttctggga
     7561 atccggctcc gaatccgaag tgggccccat gcgcttcatc gacaatcagc gccgccccgt
     7621 gtgcatgaca gcatgccgca atcgccgcga cgtccgacac gatcccctcg taggtcgggg
     7681 atgtgaggat cacaaccgca atatccggat gctttgtcag cgcctcctcc acctgcgcag
     7741 cggtaatctg tccctgcaga tctccccgcg tgattgccgg atagaggaac tccgccgcga
     7801 gtccctgcat ggaaatcgcg tgatagaccg ctttgtggca gttccttgcc gccagtacct
     7861 tatctccccg ccgtgcggca gcgcagatgg cggcaagcag tccgcaggtg ctcccgttta
     7921 ccaggtaata cgaccggtcc gcaccgtaaa gcttcgccgc gcgctcctcg gcctccctga
     7981 taagtccttc ggcatgatgc aggttgtcaa atccgtcaat ctccgtaata tcgatggtat
     8041 acggatttgg aaacggaagt gcccggcgct tgtgccccgg catatgaaaa gggtagacat
     8101 ccgattcccc gtatgaaatt agctgttgat acaaatgctc tctctccatt attcctgtca
     8161 ctttcccctg tcaatccgtc ttgttttgtc ccgtcgatcc gcgcagcaga atttttgtca
     8221 cttgaacaat ccgccgtttt ccattatgat atgggatata tttatttaat agaaatggcg
     8281 gaaattttta tgaaacacgc actcattatc ctgtgcggcg gcggaagcac gcgcatggga
     8341 accgataaag ccctgctgcc ttttggcgaa gtaagtctgc tggaatacct tgtgaacaag
     8401 tacaaaccac atttttcgca tatttactta tccgtgaaaa agccgggcga atatacacac
     8461 ctgtcactcc ccgtcacaga aattgccgat ctgtacatga acgccggtcc gatgtccggc
     8521 gtattttccg gtctctccat gatggatgaa gaccaggcgt ttattctgcc gatcgacacc
     8581 ccttttctgg aaccagaagt cggactgcag ctgctggagc atctggacgg ttttgacatc
     8641 gccgcggtat ccggcaccga aacggatcaa aaagtaccgg cgtccgccta ctccaaaagc
     8701 tgcctgccat ccatcggaaa gtgtctgctt ttgcaccagt tcactttcga tacgctgcgc
     8761 gagaaatgca gcgtgtccta tctgaagcgc gaagaagcga ccaaagactc cgatattccg
     8821 gcagatcttc agttttccca tctggatacc agggacgact actatctggc gctccggcac
     8881 cttcactgca tttaactaat ctaagaaccg ctgcagatcc ggcatccagt ctccgaaaaa
     8941 aatctcgtcc cggcacaggg tgctccacat ctgcggaagg aattgttcca gaacctgcat
     9001 gacctctgtc catgcgggtt cttttctttt ccggtgtctt aaatatttct cccaacgctt
     9061 tctcatgtag ctgtaatcgc ggtaggaaag aatctctttc atccgcgact cctcgggaag
     9121 taactgctgc tttttgcaat gttcgccgag cagttcctga atatgcctgc cgtccaccgg
     9181 ctccgtcttt aatatcccgt aaaccttatc gtagacgctc atatccggaa gcagctccat
     9241 atcccggatc agataaaaaa gattctccgc gagaatactc tccaccggat attccagata
     9301 cggaatctgc tttccgcctt ccataaacgg cataaaagtc cgcttgaccg gaaccaggcc
     9361 ttcctcggtc agttccgtga cgcggatatg gatcgggacc gtcatctcct cgaactcccc
     9421 cgcaatctcc agatccacac ggtgatctcc cgcgctcgct cttcctttcc aggaaatgtc
     9481 cggcaccttt tccactttta ggatgtacgc gagcatgaca tatcccatct taagagacag
     9541 ttcctgcccc ggaacgatcc cctcccgctt catcgcgcgg ggatctgtca cataggcaaa
     9601 atccagcgtc aacagatttt tcttccggta ctgttccacc ccgagcgcac cgctgttttt
     9661 cagccacaga aacagggaaa acggcgaatc ctcgatcaga tacatcagct cttc
//
LOCUS       DBMDQJ010000005         9367 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3288_length_9367_cov_1.440751, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000005 DBMDQJ010000000
VERSION     DBMDQJ010000005.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 9367)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 9367)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9367
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3288_length_9367_cov_1.440751"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="ACZO9R_00205"
     CDS             <1..267
                     /locus_tag="ACZO9R_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR0911524.1"
                     /translation="QFLYAGILGLLLAWLLERTGFLYAPVLAHIGANVMAVVRSETGW
                     LDIAYQPTAAGVAVTVLLFAVAAFAVLLVKKGGVRQEEEKTSAA"
     gene            636..1646
                     /locus_tag="ACZO9R_00210"
     CDS             636..1646
                     /locus_tag="ACZO9R_00210"
                     /inference="COORDINATES: protein motif:HMM:NF013741.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR0911525.1"
                     /translation="MVLIFLNAVFASAEIAVISMSDLKLRQLCEAGDRRAGKLEAFIK
                     QPAKFLATIQVAITLAGFLQSAFAAENFAQPLVTLLRGTGIPVAEEALKSACIVVITL
                     ILAYFNLVFGELVPKRVAMKKAEELALKMAGMLSLVAKIFAPLVWLLTASTNFWLRRF
                     GIDPEEEEETVSEEEIRLLLAEGREQGTIPTEETEMIQNVFGFDDIAVGTICTHRREM
                     VSLSTEEDEAVWDRILAESVHTYYPIFAGRSEDVVGILDGKAYFRMKDHTRDKVMQCA
                     VQKPMLASDSMKANRLFEQMKQQQNYFAVVTDAYGGVTGIVTLCDIMEYLVGDLENTA
                     TA"
     gene            2043..>4097
                     /locus_tag="ACZO9R_00215"
     CDS             2043..>4097
                     /locus_tag="ACZO9R_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR0911526.1"
                     /translation="MYDPGYEHDNCGIGSVVNIKGIKTHETVENALKIVENLKHRAGK
                     DAEGKTGDGVGILLQISHKFFSKAAKQLGIELGEERDYGVGMFFFPQDELKRNRAKKM
                     FEVIVGKEGMEFLGWREVPTDPTKLGQKAVDCMPYIMQGFVKRPADVEKGLAFDRKLY
                     VARRVFEQSNDDTYVVSLSSRTIVYKGMFLVEQLRLFFADLQDKDYESAIATVHSRFS
                     TNTNPSWERAHPNRFIVHNGEINTIKGNADKMLAREETMESEHLKGELQKVLPVINSE
                     GSDSAMLDNTLEFLVMSGMELPLAVMIMIPEPWANNNIMSQKKKDFYQYYATMTEPWD
                     GPASILFSDGDMMGAVLDRNGLRPSRYYITDDDYLILSSEVGVLDIDPTKIVVKERLR
                     PGKMLLVDTVKGRVIDDDELKETYANKQPYGEWLDRNLLKLEDLKIPNERVPEYTKEE
                     RQRMQKAFGYTYESLREAILPMAKNGGEGTSAMGIDTPLAALASDHQPLFNYFKQLFA
                     QVTNPPIDSIREKVVTSTTVYIGEDGNLLEERAENCKVLKVNNPILTNTDLMKIKAMK
                     VDGFKVEVLPIIYYKNTSLEKAIERLFVEADRAYREGANILILSDRGIDENHVPIPSL
                     LAVSALQQHLVKTKKRTAVAMILESGEPREVHHFATLLGYGACAINPYLAQDTVKQLV
                     DEH"
     assembly_gap    4098..4107
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4108..6572
                     /locus_tag="ACZO9R_00220"
     CDS             <4108..6572
                     /locus_tag="ACZO9R_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR0911527.1"
                     /translation="HMLDKDYYAAVQDYNAAILNGIVKIASKMGISTIQSYEGSKIFE
                     AIGINKDVIDKYFTNTVSPIGGITLEDIADDVNELHSAAYDPLGLETDLTLDSRGRHK
                     MRSGADPHLYNPATIHLLQEATKRGDYELFKQYTELVNKEEREITLRGLMNFNYPKKG
                     VPLEEVESVDSIVTRFKTGAMSYGSISKEAHETLAIAMNRLHGKSNSGEGGEDADRLA
                     SKNSPVNRCSAIKQVASGRFGVTSEYLVSAQEIQIKMAQGAKPGEGGHLPGKKVYPWI
                     AKTRLSTPGVSLISPPPHHDIYSIEDLEQLIFDLKNANRDARITVKLVSEAGVGTVAA
                     GVAKAGAQVVLISGYDGGTGAAPSSSIHNAGLPWELGLAETHQTLIMNGLRNKVRIET
                     DGKLMSGRDVATAALLGAEEFGFATAPLVTLGCVMMRVCNLDTCPAGIATQNPELRKR
                     FAGKPEYVENFMRFIAQDLREYMAKLGCRTIDEMVGRSDLLKVREDLSGRDREIDLSN
                     LLNNPFAGPKEKITFDPKHVYDFELEKSKDVTVLLKQLKGALEQKQRRSIDVEVTNTD
                     RSFGTIFGSEITKKYGGEGLPEDTFIVKCNGAGGQSFGAFIPKGLTLELVGDSNDYFG
                     KGLSGGKLVVYAPAGVKYEKDENIIIGNVALYGATSGKAFISGVAGERFCVRNSGASA
                     VVEGVGDHGCEYMTGGRVVVLGKTGKNFAAGMSGGIAYVLDEDNDLYTRMNKEMVFSQ
                     EISNKYDVMELKDMIKEHVALTNSEKGKKILDNFSEYLPKFKKVIPYDYNRMLMAIVQ
                     MEEKGLSSEQAQIEAFYANTRG"
     gene            6633..8126
                     /locus_tag="ACZO9R_00225"
     CDS             6633..8126
                     /locus_tag="ACZO9R_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HIR0911528.1"
                     /translation="MGKPTGFMEYDREDARAIEPKERIKNFNEFHIPLSKERQQIQGA
                     RCMNCGVPFCQAGMNIMGMTSGCPLHNLVPEWNDLVYTGNWEQAYNRLKKTNNFPEFT
                     SRVCPALCEAACTCGHWEDPVTCKANEHGIIENAYEQGYAAAKPPKVRTGKKIAVIGS
                     GPAGLAAADQLNKRGHSVTVYERDDRVGGLLMYGIPNMKLEKWVIDRKVAVMKEEGIT
                     FVTGTNVGKDVKASKLLKEYDRVILACGAKNPRDIKAPGRDAKGIYFAVDFLKATTKS
                     LLDSELKDGSYISAKGKKVVIIGGGDTGNDCVGTSIRHGCASVTQLEMMPQAPAKRAE
                     NNPWPEWPKVLKTDYGQEEAIAVFGHDPRIYQTTVKEFLKDKNGNLCGLVTVKLEPKR
                     DEKTGRVTMTEVAGSEQKMEADLVLIAAGFLGTEKYVADAFGVDLNGRTNVATAEGAY
                     ETSVKNVFAAGDVHRGQSLVVWAIREGREVAREVDESLMGYSYLEVQ"
     gene            8253..>9367
                     /locus_tag="ACZO9R_00230"
     CDS             8253..>9367
                     /locus_tag="ACZO9R_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911529.1"
                     /translation="MTRSTAAEATAEGSTPAEQAVGGTAAETTAEGSAPAEQAVGNTS
                     AKETLSASIEPAERSENEAETWQTGARIRIADMEPDYSDITVKNPRIGKVMRLEEEAK
                     RQSEIEKLRAKRIRQEQEYIDQMSLSVENHLRATKEEQQYNEQFEERIRTEVYRMHGI
                     SEDKLQGMTEYRNAWYQGTAFAMFFLSLVLIAICGVLHGFGSEICIFMAFYTAIEGAL
                     LSNGRKQSLFFTVLTKGVYLLLFPVMLVTFTCYELGFEEYALLVPVFTVAGVVVLMIG
                     ALSYFLYDPYRVDRRNRKKANRYIVEIEKAALKEVRLKEKAFAKQEKKQAKLTQKQEK
                     RQEKLAQKEEDRTKKAAARDERRRQRKEEWAARRARWR"
BASE COUNT         2443 a         2083 c         2896 g         1935 t
ORIGIN      
        1 cagtttttgt atgcgggcat tttaggtctg ctgctcgcat ggctgctgga gagaaccggc
       61 tttttgtatg cgcccgttct ggcgcatatc ggggcgaatg tgatggcggt tgtgcgcagc
      121 gagaccggct ggctggacat tgcctaccag cccacggcgg caggtgttgc agtgacggtg
      181 ctgctgtttg cggtggcagc gtttgcggtt ctgctggtga aaaagggcgg agtgcggcag
      241 gaagaggaga aaacgtccgc tgcctgaaac cttagcagta tctgtagagg tgcagttatt
      301 gactgtcata gtttcctgtg gtatgttaaa tgacgtgaat ggcgggatcc ccggtccatt
      361 tagcatcgca ggaaggagag tgacagtttc atggacgaca gtgcggacag tgacagcgat
      421 acacagatat gcaggctttt aaaaagagaa tacggctgct ccatgcagtg ttatgaatag
      481 gtaaaggtat ggcttaccgg aggtcttttt tggacaccgg aaggtcatac ctttttgtgc
      541 gtaaatgaca caacacgtaa caggcagcag gagggcgtat cacaggacgc cggaaacaga
      601 aaggagcaga catgttgcag cccatattct tattgatggt tttgattttt ttgaatgccg
      661 tatttgcaag cgcggagatt gcggtaatct cgatgagtga tttaaaactc cgacagctct
      721 gcgaggcggg agacaggcgc gccgggaagc tggaggcgtt cataaagcag ccggcaaaat
      781 tccttgccac gatccaggtg gcgatcacac tcgccggatt tttacagagt gcgtttgccg
      841 cggaaaactt tgcacagccg ttggtgacgc ttttgagggg caccggaata ccggttgcag
      901 aggaggcgtt aaaatccgca tgcatcgtgg tgattacatt gattttagcg tattttaatc
      961 tggtcttcgg ggaactagtg ccgaagcggg tggcgatgaa aaaggcggag gagctggcgc
     1021 ttaaaatggc aggaatgctc tccctggtgg cgaagatctt tgcaccgctg gtgtggcttc
     1081 ttaccgcatc gaccaatttc tggttaagac gttttggcat tgacccggag gaggaagagg
     1141 agacggtgtc ggaggaggaa atccggctgc tgctcgccga gggaagagag cagggaacca
     1201 ttccaaccga ggagacagag atgatacaga acgtgttcgg gtttgacgat attgcggtcg
     1261 gaacgatctg tacgcaccgc agagagatgg tatctctgtc cacggaggag gatgaagccg
     1321 tctgggaccg gattctggcg gagagcgtac atacctatta tccgatcttt gcggggagaa
     1381 gcgaggatgt cgtgggaatc ctcgatggaa aagcgtattt ccgcatgaaa gaccacacgc
     1441 gtgacaaggt gatgcagtgc gcggtacaaa aaccgatgct ggcatcggat tccatgaagg
     1501 caaaccggct ttttgagcag atgaaacagc agcagaatta ttttgcggtc gtgacagacg
     1561 cctacggcgg ggttaccgga atcgtgacgc tgtgcgacat catggagtat ctggtgggag
     1621 atctggagaa tacggctacc gcatagaccg gtgtgacaat tcagatacaa tttaagaaaa
     1681 ggcagaaaaa gagcccggaa aaagttttgc gtgcaaagtt tttttcgggc tcttatatgt
     1741 tgaattgtgt gtgagagaat tatttataca aaaaaataat aaataatatc tgataataac
     1801 aaaaaataaa catcgtatac aaataatcag acaattataa gaataaactg gtagacaaac
     1861 aaatgagaaa gttgtatatt aggatataac aaattcggta aagacaactg tccgtgcagt
     1921 aaagacagct gatcggaata cagaatgcag aaaggaagct gattatgaaa gagcagacca
     1981 cttataagaa ggacgccctc aaccgtttct cggtatcgga tgcgggaaaa caggcagccg
     2041 gaatgtacga tccgggttac gagcacgata actgtggtat cggttcggtt gtcaacatta
     2101 agggaatcaa gacacacgag actgtggaga atgcgttaaa gatcgttgag aacttaaagc
     2161 acagagcagg caaggatgcg gagggaaaga ccggtgacgg tgtcggtatt ctgctgcaga
     2221 tatcccacaa gtttttttcc aaggcggcaa aacagcttgg aatcgaactc ggtgaggaga
     2281 gagactatgg tgtcggcatg ttctttttcc ctcaggacga gttaaagcgc aaccgtgcca
     2341 agaagatgtt cgaggtcatt gtcggcaagg agggaatgga gttcctcggc tggcgtgaag
     2401 ttccgaccga tccgacgaag ctgggacaga aagcggtgga ctgcatgccg tatatcatgc
     2461 agggttttgt gaagcgtccg gcggatgtgg agaagggact ggcgtttgac cgcaagctgt
     2521 atgtggcgcg aagagtcttc gagcagtcca acgacgatac ctatgtggta tccctttcca
     2581 gccgtacgat cgtctataag ggaatgtttc ttgtggagca gcttcgtctg ttttttgcgg
     2641 atttacagga taaggattac gagtcggcga ttgcaaccgt gcattcccgt ttctccacga
     2701 acacgaaccc gagctgggag agagcgcacc cgaaccgttt tatcgtacat aacggtgaga
     2761 tcaatacgat caagggcaac gccgataaga tgctcgcccg tgaggagacg atggagtccg
     2821 agcacttaaa aggcgaactt cagaaagtgc ttccggtcat caacagtgag ggatcggatt
     2881 ccgcgatgct ggacaatacg ctggagttcc tcgtgatgag tggtatggag cttccgctcg
     2941 cagtcatgat catgatcccg gagccgtggg caaacaacaa tatcatgtcg cagaagaaaa
     3001 aggattttta ccagtattac gcgacgatga cggagccgtg ggacggaccg gcatccatcc
     3061 tcttttccga tggcgatatg atgggagccg tgcttgaccg caatggtctg cgtccttccc
     3121 gttactatat tacggatgat gattatctga tcctctcctc cgaggtcggc gtgctggaca
     3181 tcgacccgac gaagattgtg gtaaaggagc gtctgcgtcc gggcaagatg ctgctcgtgg
     3241 ataccgtcaa gggaagagtc atcgatgacg acgaactcaa ggagacttac gcgaacaagc
     3301 agccatacgg tgagtggctt gaccgcaacc tcttaaaact cgaggattta aagatcccga
     3361 atgagcgtgt gccggagtat accaaggaag agcgccagcg catgcagaag gcattcggtt
     3421 atacctacga atctctgcgc gaggcgatcc tgccgatggc gaaaaacggt ggcgagggta
     3481 catccgccat gggtattgac acaccgcttg cagcgcttgc gagtgaccat cagccgctgt
     3541 ttaattactt caaacagctg tttgcgcagg tgaccaaccc gccgatcgac tcgatccgtg
     3601 agaaagtcgt gacctctacg accgtctaca tcggcgagga cggcaacctc ttggaggagc
     3661 gggcggagaa ctgtaaggta ttgaaggtca acaacccgat tctgaccaac accgacctga
     3721 tgaagatcaa ggcgatgaag gtcgacggat tcaaggtaga agtactgccg attatctact
     3781 ataagaatac aagcttggag aaggcgatcg agcgtctgtt tgtggaggcg gaccgtgcgt
     3841 accgtgaggg agcgaacatt ctgatcctct ccgaccgcgg catcgacgag aaccatgtgc
     3901 cgatcccgtc actgctcgct gtatcagcac tgcagcagca tctggtaaag acgaaaaaga
     3961 gaacggcggt tgccatgatc ttagagtccg gagagcctcg tgaggtacac cattttgcga
     4021 cgctcctcgg ctacggagcc tgtgcgatca acccgtatct ggcacaggat accgtaaaac
     4081 agctggtgga cgagcatnnn nnnnnnnagc atatgctgga caaagattat tatgcggcgg
     4141 ttcaggatta caatgcagcg attttaaacg gcatcgtcaa gatcgcatcc aagatgggaa
     4201 tctcgacgat ccagtcctac gagggttcca agatcttcga ggcgatcggt atcaataagg
     4261 atgtgattga caagtatttc acgaatacgg tcagcccgat cggcggcatt acgctggagg
     4321 atattgcgga tgacgtcaat gagcttcact cggcggcgta cgatccgctg ggattagaga
     4381 cggatctcac attggacagc agaggacgcc acaagatgag aagcggcgca gatccgcatc
     4441 tctacaatcc ggcgacgatt catctgcttc aggaggcgac aaagcgcggc gactacgaac
     4501 tctttaaaca gtacacagag cttgtgaata aagaagagag agagatcacg ctccgcggtc
     4561 tgatgaattt caattacccg aaaaagggcg tgccgctcga ggaggtcgag agtgtcgatt
     4621 ccatcgtgac gcgttttaag acgggggcaa tgtcctacgg ttccatctcc aaggaggcgc
     4681 acgagacgtt ggcgatcgcc atgaaccgtc tgcacggcaa gtccaactcc ggcgaaggag
     4741 gagaggatgc agaccgtctt gcgagcaaga acagcccggt caaccgttgc tctgcgatca
     4801 agcaggtggc gagcggacgt ttcggtgtga ccagtgagta tctggtgagc gcgcaggaga
     4861 ttcagatcaa gatggcacag ggcgcaaagc cgggcgaggg cggacacctg ccgggcaaga
     4921 aggtctatcc gtggattgca aagaccagac tttccacacc gggtgtatca ttgatctccc
     4981 cgccgccgca tcatgacatc tactcgatcg aggatctgga gcagttgatc ttcgacttaa
     5041 agaatgcgaa ccgtgatgcg cgcatcaccg tgaaactggt atccgaggcg ggagtcggta
     5101 cggtcgcagc cggcgttgca aaggcaggcg cacaggttgt cctgatctcc ggctatgacg
     5161 gcggtaccgg tgcggctccg tccagctcga tccacaatgc gggacttccg tgggagcttg
     5221 gtcttgcgga gacacatcag acattgatta tgaacggact gcgcaacaaa gtacgcattg
     5281 agacggacgg caagctgatg agcggccgcg atgtcgctac ggcagcgctg ctcggagcgg
     5341 aggagttcgg atttgccaca gcgccgcttg tgaccttagg ctgcgtgatg atgcgcgtct
     5401 gtaacctgga tacctgtccg gcaggtattg cgacacagaa cccggagctt cggaagcgtt
     5461 ttgccggcaa gccggaatat gtggagaatt ttatgcgctt tatcgcacag gatctgcggg
     5521 agtatatggc aaagctcgga tgccgcacga tcgacgagat ggtcggcaga agcgatctgc
     5581 ttaaggtgcg cgaggatctt tcgggacgcg accgcgagat cgatctctcc aatcttttga
     5641 acaacccgtt tgcgggaccg aaggagaaaa taacctttga cccgaagcat gtctatgatt
     5701 ttgaactgga gaagtccaag gatgtgaccg tgctcttaaa acagttaaag ggtgcgttag
     5761 agcagaagca gcgcagaagt attgatgtgg aggtgacgaa caccgaccgt tccttcggta
     5821 cgattttcgg ttcggagatc acgaagaagt acggcggaga gggactgccg gaggatacct
     5881 tcattgtaaa atgcaacggt gccggcggac agagttttgg agcttttatc ccgaagggac
     5941 tgacgttaga gctggtcggc gattccaacg attactttgg aaaaggatta tccggcggca
     6001 agcttgtcgt ctatgcaccg gcgggcgtga agtatgagaa ggatgagaat atcatcatcg
     6061 gtaatgtggc gctctacgga gcaaccagcg gaaaagcgtt tatcagcggc gtcgcaggcg
     6121 agcgtttctg cgtgcgcaac tcgggagctt ccgcagttgt ggagggcgtc ggagaccacg
     6181 gatgtgagta tatgacgggt ggccgtgtcg tcgtgctcgg caagacgggc aagaactttg
     6241 cagccggcat gagcggcggt atcgcttatg tgctcgacga ggacaatgac ctctacacaa
     6301 gaatgaataa ggaaatggta ttcagccagg agatttccaa caaatatgat gtcatggaat
     6361 taaaggacat gatcaaggaa catgtggcgc tgaccaactc ggaaaagggc aagaagatcc
     6421 tggataattt cagcgagtat ctgccgaagt tcaagaaagt cataccgtat gactataacc
     6481 gtatgcttat ggcgattgtt cagatggagg agaaaggtct ttcttccgaa caggcacaga
     6541 ttgaagcttt ttatgcaaat acgcgtggat agtgtgtgag caggtgtcgt ttgacatgtg
     6601 tgactcatgc attttcgaga aaggatagaa ttatgggaaa accaacagga tttatggaat
     6661 atgacagaga agatgcccgc gccattgagc cgaaggagcg gatcaaaaat tttaatgagt
     6721 tccacattcc tctgtccaaa gagagacaac agattcaggg cgcgcgctgc atgaactgcg
     6781 gcgtgccgtt ctgccaggcg ggaatgaaca tcatggggat gacctcggga tgcccgctgc
     6841 acaacctggt tccggagtgg aatgatcttg tatacacggg gaactgggag caggcataca
     6901 accgcctgaa gaagaccaac aatttcccgg agtttacctc ccgggtatgt ccggcactct
     6961 gcgaggctgc ctgcacctgc gggcactggg aagatccggt aacctgcaag gcgaacgagc
     7021 acggcatcat cgagaatgcg tatgagcagg gttatgcggc ggcaaagcct ccgaaagtcc
     7081 gcaccggcaa gaagatcgcg gtgatcggct ccggtcctgc ggggcttgcg gcggcggatc
     7141 agctcaacaa gcgcggacat tccgtgacgg tctatgagcg ggacgacaga gtcggcggac
     7201 ttctgatgta cggaattccg aatatgaagc tggaaaaatg ggtcatcgac cgcaaggttg
     7261 ccgtcatgaa ggaggagggc atcacctttg tgaccggcac caatgtcgga aaggatgtaa
     7321 aggcctccaa gctgttaaag gagtacgacc gtgtgatttt agcgtgcggc gccaagaatc
     7381 cgagagacat caaggcaccg ggacgggacg ccaagggaat ctattttgcc gtggattttt
     7441 taaaagcaac gacgaagagt ctcctggatt ctgagctaaa agacggcagc tatatctccg
     7501 caaaagggaa aaaagtggtc attatcggcg gcggcgatac cggaaatgac tgcgtgggca
     7561 cgtccatccg ccacggctgc gcgagcgtga cacagcttga gatgatgccg caggcaccgg
     7621 caaagcgcgc ggagaataat ccgtggccgg agtggccgaa ggtgttaaag accgattacg
     7681 gccaggagga agcgattgcg gtgttcgggc acgacccgcg gatctatcag acgacggtga
     7741 aggaattctt aaaagacaag aacggcaatc tctgcgggct tgtcactgta aagctcgaac
     7801 cgaagcggga tgagaagacc gggcgtgtga cgatgaccga ggttgcggga agcgagcaga
     7861 agatggaagc ggatcttgtg ctgatcgctg ccggattcct cggcacggaa aaatatgtgg
     7921 cggacgcgtt cggtgtggac ttaaacggca gaaccaacgt cgcaacggcg gagggcgcct
     7981 atgagaccag cgtgaagaat gtgttcgcgg cgggcgatgt gcacagagga cagtcccttg
     8041 ttgtctgggc gatccgcgag ggtcgcgagg tcgcgcgcga ggtcgatgag agcctgatgg
     8101 gttattcgta tctggaggtg cagtaatcga ataagaatcc cccgtttgta atccaacgga
     8161 ttctgtgcta agatgagaaa aagagcacgc acgaagaaaa ggggagtagg atgagccgca
     8221 gaaggaaaaa agcaggggaa aaggcacgga cgatgacgcg aagcacagca gcagaagcga
     8281 cagcagaggg aagtacaccg gcagagcagg cggtgggagg cacagcagcg gaaacgacgg
     8341 cagagggaag tgctccggca gagcaggcgg taggaaacac atcggcgaaa gagacgttat
     8401 cggcgagcat agagccggcg gagagatcgg aaaatgaggc ggaaacctgg cagaccggcg
     8461 cgcggatccg gattgcggat atggagccgg attactcgga tatcacagtg aagaatccgc
     8521 gcatcggcaa ggtcatgcgc cttgaggaag aggcaaagag acagagcgag attgagaagc
     8581 tgcgtgcaaa aagaatccgg caggagcagg aatatattga ccagatgagt ctgtctgtcg
     8641 agaaccatct gcgcgccaca aaggaggaac agcagtacaa cgagcagttt gaggagcgca
     8701 tccgcacgga agtgtaccgg atgcacggga tcagcgagga caagctgcag ggcatgacgg
     8761 agtaccgcaa tgcatggtat cagggcacgg cgtttgctat gttttttctg tcgcttgtgc
     8821 tgatcgccat ctgcggcgta ttgcacgggt tcgggtcgga gatctgcatt tttatggcgt
     8881 tttataccgc tattgagggc gcactgctct ccaacgggag aaagcagtcc ctgtttttta
     8941 ctgttctgac gaaaggcgta tacctgctgc tgtttccggt catgctcgtg acgtttacct
     9001 gctacgaact tggttttgag gaatatgcgc ttctggtacc ggtttttacc gtggcaggtg
     9061 ttgtggtgct catgatcgga gcgctgtcct attttctgta tgatccctat cgtgtggatc
     9121 gcaggaaccg caagaaggcg aaccgctata tcgtagagat tgagaaggcg gcgttaaagg
     9181 aagtgcggct gaaggaaaag gcgtttgcaa agcaggagaa aaagcaggcg aagcttaccc
     9241 agaagcagga aaagcgtcag gaaaagcttg cccagaagga agaggacagg acaaaaaagg
     9301 cggctgcacg ggatgaacgg cgcaggcagc ggaaagagga gtgggcggca agacgtgcac
     9361 ggtggcg
//
LOCUS       DBMDQJ010000006         8934 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3453_length_8934_cov_1.620740, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000006 DBMDQJ010000000
VERSION     DBMDQJ010000006.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 8934)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8934)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8934
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3453_length_8934_cov_1.620740"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="ACZO9R_00235"
     CDS             <1..846
                     /locus_tag="ACZO9R_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HIR0911530.1"
                     /translation="EEYRSQNEELARYLEETGHKVLWSNDIYEKNAAFSGTGAERAAE
                     LMRFFNDPEVEEIYDISGGDLANQVLDGLDYAAIRRSRAVFWGYSDLTTVINAIYAQT
                     GKSSVLYQVKNMVWGEVKEVQRRRFENRAALFDPQVRLVQGEKMEGVVVGGNIRCFLK
                     LSGTRYFPDLCDKILLLEAYGGEVPQMATYLAQLKQLGAFEKVGGILLGTFTAMEAHA
                     CSPDITALVREAAGTQIPIAKTQEIGHGNDAKAIRIGERIYCGGDEACRSVVTDAERR
                     QLETR"
     gene            843..1703
                     /locus_tag="ACZO9R_00240"
     CDS             843..1703
                     /locus_tag="ACZO9R_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0911531.1"
                     /translation="MKKEMTYTELAKEMAILTAAVAIIATAVYFFLVPSHASVSSISG
                     LGIVLSNFIPLPLSAITMILNVVLLIIGFFTCGREFGVKTVYTSVALPVFLGIFERIF
                     PDYESLTGSQELDVLCYILVVSVGLSILFNRNASSGGLDIVAKIMNKYLHMELGKAMS
                     LSGMCVALSAALVYDKKTVVLSILGTYFNGIVLDHFIFDHDMKRRVCIITEKEEELRK
                     FIIEDLHSGATIYESIGAYNMQKRNEIIAIVDKAEYQKLMNYISREDPHAFITVYTVS
                     DIRYQPKRQQ"
     gene            1742..2440
                     /locus_tag="ACZO9R_00245"
     CDS             1742..2440
                     /locus_tag="ACZO9R_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911532.1"
                     /translation="MEIYDNADSSYKTAGAETYHLPVLYTEDERSSYAVWGIKKEKLL
                     LPFAVVTIIIDAFVLCATVVYLFVVQRQTPAFSVWISCWGGLVGDVSYGVALVLTVLI
                     LKPLDWLLDKIMHRPEEPKMLQVTPKETGVSYFLLRGTTVLAQGMLTWDAWEAALTVE
                     TNEILLEGQRLRIGSNTIASIYPPEKQKPWMDHPAEQITGTISLAHIWKNMQGYRASL
                     EEQKREAEWRRQNP"
     gene            2482..3510
                     /locus_tag="ACZO9R_00250"
     CDS             2482..3510
                     /locus_tag="ACZO9R_00250"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 family peptidase"
                     /protein_id="HIR0911533.1"
                     /translation="MRYPKNLSEHGTIGFVAPSFGCATEPYHTAFLNAQKKFREMGHP
                     LDLGPNCYADCGIGISNTPEACGRELTEYYCSDKNEVLISCGGGELMCETLDAVDFEQ
                     IRAAVPKWYMGYSDNTNMTYLLATLCDTASIYGPCAAAFGMEPWHQSLSDAYALLRGQ
                     KHTVNGYDLWEKESLKDTEHPLVPYHVTEQRILHVFPEPTEALTVQGRLIGGCVDCLV
                     NLLGTTYDKTTGFVEKYKNDGMIWFLESCDLNVMAIRRAVWQMKHAGWFSHVKAFLIG
                     RPAVYGQELMGLDQYHAVWDLLKDYGVPVIMDVDIGHLAPMMPLVCGSYATVQVNGND
                     ISVKMEYL"
     gene            complement(3505..4224)
                     /locus_tag="ACZO9R_00255"
     CDS             complement(3505..4224)
                     /locus_tag="ACZO9R_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911534.1"
                     /translation="MSNTAYIGSGTTLSSKYYLRSFYRSNREAGTSSKRREFSGNQLA
                     LADGRALRQAVRRLTSSDFSDDQDTNTRNSVLAYIQTYNNMLSSAGSSSDRTLERSAK
                     QLKNITSEYSSELDKIGITINDDGTLTSRTTLFESADLSKFKELFSADAAYMQRTSTY
                     AKRFASRGEALVASDNNRLMQKKNAAATGSSATDGTTTSGATESPDDGTATTAAQIVS
                     QSLDLDTLLNTGIGKNINIIL"
     gene            4393..4872
                     /locus_tag="ACZO9R_00260"
     CDS             4393..4872
                     /locus_tag="ACZO9R_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR0911535.1"
                     /translation="MEAMIWIGLIILFVVVEIATVGLTSIWFAGGALISLILCLCGVG
                     LGWQIGVFFVVSMILVAFTRPWAVKYLKPHLIRTNYEQAIGENVCLTETVDNQRGTGT
                     AVYKGQEWTARASEDGKRYEAGTIVKVKEIRGVTMYVTESGLMPQVPIPPQTEQEAE"
     gene            4874..5722
                     /locus_tag="ACZO9R_00265"
     CDS             4874..5722
                     /locus_tag="ACZO9R_00265"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR0911536.1"
                     /translation="MKLGMVFEGGANRTVFSCGVMDAFLKERLMPDYLIGVSAGIAYG
                     VSYLSGQKGRNIEVVENYIADKRYMGMRHLFNRNEKAYFNTKFVFEEVPNELIPFDYD
                     AFAAYPGKVEAAVTNIHTGKAEYVEVPRGRDMRDTLVASCSLPILFQPVKLGRHYYLD
                     GGIADSIPYEHALAEGCDRLIVVLTRERGYVKKTEKAVRLTDKLYKKYPKIVESMDSR
                     AERYNECMTRLQEFEQEGKIFVIAPESTYGVGRTTTDVIKLRRLYDEGYELTEKRMQE
                     LRAYLG"
     gene            5742..6677
                     /locus_tag="ACZO9R_00270"
     CDS             5742..6677
                     /locus_tag="ACZO9R_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HIR0911537.1"
                     /translation="MKIVFLDAKTIGDDIDLSGYDALGEVVKYGFSTPEEARERTKDA
                     DVIVLNKVVINEQSIGEAEHLKLVCVTATGTNNLDKEYLDQRGIAWRNVAGYSTETVA
                     QHTFALLFYLLEKLHYYDEYVKSEKYVGDVSFTHFDNVFHELSGKTWGVIGLGAIGRR
                     VADIAGMFGCRVLYYSTSGKHDQPGYERVDFDTLLAESDIVSVHAPLDANTEGLMNAG
                     AFAKMKKSAIFLNLGRGPIVVEKDLAEALKSGTIAAAGLDVLSAEPMRADNPLREIKD
                     SDKLMITPHIAWASIEARTRLMKIIEGQIREFFHL"
     gene            complement(6703..7671)
                     /locus_tag="ACZO9R_00275"
     CDS             complement(6703..7671)
                     /locus_tag="ACZO9R_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR0911538.1"
                     /translation="MPGFVTHYLFGEEIYHQLKCNSQKKNLFYNRSAYAFGLQGPDLF
                     FYYLPSYALHRHNLGALAHTTEPRAFFHGLLESYGRLSSAADRGIAEAYISGFLGHYL
                     LDTTCHPYIYAMTHYHDKKEKSYFSRHAYLETEIDTELLARKRHKTPCEFHAADTIQL
                     TFAQKRVIAGLLYDAYHYAFPELSIHRSTMWLGIISLPLGMRILHDETGQKKVLFRFM
                     EKHFLGYPLFSPLIPSDTLFFRTDPFNLRHASWKNPWDATRVSTESFFDLYENSSARC
                     LTMMRKLHTLLHTEPKSELQKQLTLEFLKEYGNLSFHSGLDARIPS"
     gene            8004..>8934
                     /locus_tag="ACZO9R_00280"
     CDS             8004..>8934
                     /locus_tag="ACZO9R_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR0911539.1"
                     /translation="MANLAQSVERKAFSVAIDAALKHLNKDREKGLLQIVDLAEKFMG
                     DNFRKEAYDGARKMIQDPDNKWMHYVNRLLDETDPHVAKMTALNLGYQAAFVGTKTIR
                     KMREVHNCNIPWLILMDPTSACNLRCTGCWAAEYGHKLNLTFDELDNIVTQGKELGIY
                     FYMMTGGEPLVRKDDIIKLCEKHNECAFHCYTNGTLVDQAFCDEMKRVGNLSLSISLE
                     GFEEANDFRRGEGVFDKVLHAMDLLHENGLIFGNSVCYTSKNMDAVTSDEFFDLLIEH
                     GSRFAWYFHLMPVGMKASPELMPTREQREYIYHR"
BASE COUNT         2342 a         1854 c         2657 g         2081 t
ORIGIN      
        1 gaagaatatc gaagccagaa cgaggaactg gcacggtatc tggaggaaac gggacacaag
       61 gttttatgga gcaatgatat ctatgaaaaa aatgccgcat tttcaggtac gggagcagag
      121 cgcgccgcgg aactgatgcg tttcttcaat gacccggagg tggaggagat ctatgacatt
      181 tccggaggag atctcgctaa tcaggtgctg gacgggctgg actatgccgc catccgaaga
      241 agcagggcgg ttttctgggg ctacagcgat ctgacgacgg tcatcaacgc catctatgca
      301 cagaccggaa aaagcagcgt gctctatcag gtcaagaaca tggtctgggg agaggtaaaa
      361 gaggtgcagc ggcggagatt tgagaaccgt gcggcgctgt ttgacccgca ggttcgcttg
      421 gtgcaggggg agaagatgga aggcgttgtt gtcggcggga atatccgctg ctttttgaag
      481 ctgtcgggaa cccgctattt tccggatctt tgcgacaaga ttctgctgct ggaagcatac
      541 ggcggcgaag tgccgcagat ggcgacgtat ctggcgcagc taaagcagct gggggcgttt
      601 gagaaggtcg gaggaatttt gctcggaaca tttacggcga tggaggcgca tgcgtgcagc
      661 ccggatatca ctgcattggt gagggaggcg gcagggacgc agattccgat cgcaaagaca
      721 caggaaatcg gtcacggaaa tgacgcgaaa gcgatccgga tcggagaaag gatatactgc
      781 ggcggagacg aagcatgtcg aagcgttgtt acagatgctg aaaggagaca gttggaaacc
      841 agatgaaaaa agagatgaca tacacagaac tagcgaaaga gatggcaatc ctgacggctg
      901 ccgtggcgat tatcgccaca gccgtatact tttttctggt gccgagccac gcatccgtca
      961 gcagcatttc cggtcttgga atcgtgctgt ccaattttat accgctgccg ttgtccgcga
     1021 tcacgatgat cttaaatgtg gtgcttttaa ttatcggatt ttttacctgc ggaagggaat
     1081 ttggcgtgaa gacggtttac accagtgtgg cgctgccggt gtttctggga atttttgaga
     1141 gaatcttccc ggattacgaa tcgctcaccg gcagccagga actggatgtg ctgtgctata
     1201 ttctggtggt cagtgtggga ctcagcattc tgtttaaccg gaatgcgtct tccggcggac
     1261 tggacattgt ggcaaagatc atgaacaaat atctgcatat ggagctggga aaagccatgt
     1321 cgctgtccgg catgtgtgtg gcgctttcgg cggcactcgt ctatgacaag aagacggttg
     1381 tgctcagtat cctcggtaca tatttcaacg gtattgtgct ggatcatttt atttttgacc
     1441 atgacatgaa gcgccgtgtc tgcatcatca ccgaaaagga agaagaactg cgcaagttta
     1501 tcatagagga tctgcacagt ggagcgacga tctatgagtc gatcggtgca tacaatatgc
     1561 agaagcggaa cgaaatcatc gccatcgtgg acaaggcgga gtatcagaaa ctcatgaatt
     1621 atatcagccg tgaggaccca catgcattca ttacggtata cacggtgtca gatatccgct
     1681 atcagccgaa gcggcagcaa tgaagaatgc gatacaactg ttgtaaaagg gagaaacata
     1741 catggaaatc tacgataacg cagacagttc atacaaaact gccggggcgg aaacctacca
     1801 tctgccggtt ctgtatacgg aggacgagcg cagcagctac gccgtatggg gcatcaaaaa
     1861 agaaaaactg ttgctgccgt tcgctgttgt gacgatcatc attgacgcgt ttgtgctctg
     1921 tgccacggtc gtttatctgt ttgtggtaca gcggcagacg ccggcatttt ccgtctggat
     1981 ttcgtgctgg ggcggtctgg tcggtgatgt ctcctacggt gtggcgctgg ttttgacggt
     2041 tcttatttta aagccgctcg actggctgtt ggataaaatc atgcacagac cggaggaacc
     2101 aaagatgctg caggtgacgc cgaaggaaac gggcgtctcg tatttcctgc ttcgcggtac
     2161 aacggtactt gcgcagggaa tgctcacgtg ggatgcgtgg gaggctgcgc tcactgtgga
     2221 gacgaatgag atattgctcg aggggcagag actgcggatc ggaagcaata cgatcgcatc
     2281 gatctatccg cctgagaaac agaagccgtg gatggatcat ccggcggagc agataacggg
     2341 aaccatcagc ctggcgcata tctggaaaaa tatgcagggg taccgggcgt ccctggaaga
     2401 acagaaaagg gaagcagagt ggagacggca gaatccatag atctgccggt ggaaaataaa
     2461 agaaaatgga gacagacaca gatgagatat ccgaaaaatt tatccgaaca tggaacgatc
     2521 ggttttgtgg cgccatcctt cgggtgtgcg actgagccgt atcacactgc atttttgaac
     2581 gcacagaaaa aattccggga aatgggacat cccctggatc tcggaccgaa ttgttatgcc
     2641 gactgcggaa ttgggatcag caacacgccg gaggcgtgcg gcagagaact gacagagtat
     2701 tattgcagcg ataagaatga ggtgctgatc tcctgcggtg gcggggaact gatgtgtgag
     2761 acactggacg cggtggattt tgaacagatc cgggcagcgg taccgaagtg gtacatggga
     2821 tattccgaca atacgaacat gacatatctt ctggcgacgc tctgcgatac cgcttcgatc
     2881 tatggcccgt gtgcggcggc gttcggcatg gagccgtggc atcagagtct ttcggatgca
     2941 tatgcacttc tgcgcggaca gaagcatacg gtaaacggat atgatctctg ggaaaaggaa
     3001 agcttaaaag ataccgagca tccgcttgtg ccataccatg tcacggaaca gcggattttg
     3061 catgttttcc cggaaccgac ggaggcactt acggtgcagg gacgcctgat cggcggatgt
     3121 gtggactgtc tggtgaatct tctggggaca acttatgata aaacaaccgg gtttgtggaa
     3181 aagtataaga atgacggtat gatctggttc ctggaatcct gtgatcttaa tgtgatggcg
     3241 atccggcggg cggtctggca gatgaagcat gccggctggt tttcccatgt gaaagctttt
     3301 ctgatcggaa gacctgccgt ctacgggcag gaactgatgg ggctggacca gtaccatgcg
     3361 gtgtgggatc tgttgaaaga ttacggggtg ccggtcatta tggatgtgga tatcggacac
     3421 ctcgcaccga tgatgccgct ggtgtgcgga agctatgcaa cggtgcaggt gaacggaaat
     3481 gacatctccg tgaagatgga gtatttatag aataatgtta atatttttgc cgatgccggt
     3541 gtttaagagg gtatccagat ccagactctg ggatacgatc tgtgccgcag tggtggcagt
     3601 gccgtcatcc ggactctctg tggcaccgga tgtggtggtg ccgtctgttg cggaagaacc
     3661 tgtggcagcg gcattttttt tctgcatcag ccggttgttg tcagaagcga cgagcgcctc
     3721 gccgcggctt gcaaaccgtt tggcatatgt tgaggtgcgc tgcatgtaag ccgcatcagc
     3781 ggaaaaaagt tccttgaact tggagagatc cgcactctca aataaggttg tacggcttgt
     3841 gagtgtgccg tcatcattga tggtaatgcc gatcttatcg agttcggaag agtattcgct
     3901 ggtaatattt ttcaactgct ttgcactgcg ctccaaggta cggtccgagg aactgccggc
     3961 agaggaaagc atgttgttgt aggtctggat ataggcgagc acgctgttgc gtgtgttggt
     4021 gtcctggtca tccgaaaaat cagaggaagt cagccggcgt actgcctgac gaagcgccct
     4081 gccgtctgcg agagcgagct ggttgccact gaattcacgg cgtttggagg aagtcccggc
     4141 ttcccggttg gaacggtaga aactgcgcag gtaatatttg gaacttaagg tggtgccgga
     4201 tccgatatac gctgtatttg acatagtgat tcctccttaa aaagattctc taatatgttt
     4261 cggcagaatg cggcatagaa ttaaccatag aaaacaaaaa acggatatgc tatactaaat
     4321 tccaaagaat tcagtctggg aacagaaact actgaattct ggcgactggg atgtatgaga
     4381 ggaggatatc atatggaagc aatgatctgg ataggtctga ttattctttt tgtggtggtt
     4441 gagattgcga cggtaggact gacgtccatc tggttcgcag ggggcgcgct gatttccctg
     4501 attctgtgcc tgtgcggcgt gggactgggc tggcagatcg gcgtattttt cgtggtctca
     4561 atgattctgg tggcatttac ccggccgtgg gcggtgaagt atttaaagcc gcacctgatc
     4621 cggaccaact acgagcaggc aatcggtgaa aatgtatgtc tgacggagac ggtagacaac
     4681 cagagaggaa ccggcactgc agtctacaag ggacaggagt ggacggcaag agccagtgag
     4741 gatggaaaga gatatgaggc gggaacgatc gtgaaggtaa aggagatccg cggtgtgacc
     4801 atgtatgtca ccgaaagcgg actgatgccg caggttccga tcccgccgca gacagagcaa
     4861 gaggcagaat aatatgaagc ttggaatggt atttgaggga ggcgccaacc gtacggtgtt
     4921 ctcctgcgga gtgatggatg cgtttcttaa ggagcggctg atgccggatt atctgatcgg
     4981 cgtctccgcc gggattgcat acggtgtatc ctacctgtcg ggacagaagg gcaggaatat
     5041 agaggttgtg gaaaattata tcgcggacaa gcggtatatg ggcatgcggc atctttttaa
     5101 ccggaacgag aaagcgtatt tcaatacgaa gttcgtgttt gaggaagtgc cgaatgaact
     5161 gattccgttt gactatgacg cgtttgcggc gtatccggga aaagtcgaag cagcagtgac
     5221 caacattcat accggaaaag ccgaatatgt cgaagtgccg aggggacggg acatgcgcga
     5281 tacgctggtg gcgagctgtt cgctgccgat ccttttccag ccggtgaagc ttggcagaca
     5341 ttattatctg gacggcggga ttgccgattc catcccgtat gagcacgcgc tggcggaggg
     5401 atgcgacaga ctcatcgtgg tgctcacaag agagcgcgga tatgtgaaga agacggagaa
     5461 ggcagtccgc cttacggaca agctgtacaa aaaatatccg aagattgtgg agagcatgga
     5521 ttcccgggcg gagcgctaca atgaatgtat gaccagactg caggaattcg agcaggaggg
     5581 aaaaatcttc gtcatcgctc cggaatccac ctatggggtg ggacggacga ccacggacgt
     5641 catcaagctc cggaggcttt acgacgaggg ttatgaactg acagaaaagc gcatgcagga
     5701 actgcgtgca tatctgggat aagaaagaga ggaaacaaac gatgaagatc gtatttctgg
     5761 atgcaaagac gatcggtgat gacatcgatc tgtccggtta tgacgcgctg ggcgaggttg
     5821 taaaatacgg attttccaca cccgaggagg caagagaacg caccaaagac gcggatgtga
     5881 ttgttctcaa caaggttgtg atcaacgagc agtccatcgg tgaagcggag cacttaaagc
     5941 tggtatgtgt gacggcgacg ggaaccaaca atctcgacaa ggaatatctg gatcagcgtg
     6001 ggattgcatg gcgcaacgtg gcgggctatt ccacggagac agtggcacag catacgtttg
     6061 cattattgtt ctatctgctg gaaaagttac attattatga tgaatatgta aaatcagaaa
     6121 aatatgtggg ggatgtttcc tttacacatt ttgacaatgt gtttcatgag ctgagcggga
     6181 agacatgggg cgtcatcggc cttggcgcga tcggcagaag agtggcggac attgcgggca
     6241 tgttcgggtg ccgggtgctc tactattcca cctccggaaa acacgatcag ccgggatacg
     6301 agagagtaga ttttgatacg ttgctcgcgg agtcggacat cgtgtcggtg catgcgccgt
     6361 tggatgccaa tacggaaggg cttatgaacg ccggagcgtt tgcaaaaatg aaaaaatctg
     6421 cgattttcct gaatcttgga agagggccga ttgtggtgga gaaagatctc gcggaggcgt
     6481 taaagagcgg tacgatcgct gcagcaggac tggatgtgct ctcggcggag ccgatgcggg
     6541 cagacaatcc actgcgtgag atcaaggaca gtgataagct gatgattacg ccgcatatcg
     6601 catgggcgag catcgaggcg cgcacgcgtc tgatgaagat cattgaggga cagatccggg
     6661 aatttttcca tctgtaaaag aatgcgccca tttgggggcg catcagcttg gaatccgcgc
     6721 atccagaccg ctgtggaagg acagattgcc gtattctttt agaaattcca gagtgagctg
     6781 tttctgcagc tcactttttg gttctgtatg cagtaatgtg tgaagctttc gcatcatggt
     6841 aaggcagcgc gcactgctgt tttcgtacag atcaaaaaag ctttccgtgg agacgcgtgt
     6901 ggcatcccag ggattcttcc aggaggcatg cctcaggtta aaaggatccg tgcggaaaaa
     6961 cagtgtgtcg ctcggtatca acggggaaaa taacgggtat cccagaaaat gcttctccat
     7021 gaaccggaaa agaacctttt tttgtccggt ttcatcgtga agaatccgca tgccgagcgg
     7081 aagcgagatg attcccagcc acatggtgct gcgatgaatg gaaagttcgg ggaatgcgta
     7141 atgataggca tcgtacagga gcccggcaat cacgcgtttc tgggcaaaag tcagctggat
     7201 cgtatcggcg gcgtggaatt cacagggtgt cttgtggcgc tttctggcaa gcagttcggt
     7261 gtcgatctct gtctcgagat aggcatgtct ggaaaaatag cttttctcct tcttgtcatg
     7321 gtaatgcgtc atggcataga tatagggatg gcatgtggtg tcgagcagat agtgccccag
     7381 gaaaccggag atgtaggctt cggcaatgcc ccggtctgcg gcggacgaga gacgcccgta
     7441 actttccagc agtccgtgaa aaaaggcacg cggctccgtg gtgtgtgcga gggcaccgag
     7501 attgtggcgg tgcagggcat aggacggaag ataatagaaa aacagatccg gtccctgcag
     7561 tccgaacgca taagcgctgc ggttataaaa cagatttttt ttctgggaat tacattttaa
     7621 ctggtgatag atttcttcgc caaacagata atgtgtaaca aaacctggca tgggaattcc
     7681 tcctttcgtt catcatgagt ataaccattt tacaggaagg tgttaagtga ggaatggaag
     7741 atttaagaaa aatgtaataa aacaaataga ttgacaatca aaaaaagagt gttatactat
     7801 cacgtagtaa tggacactat ataatatagt tcggcgataa tcaaaacagt cagattgtca
     7861 taagatgata gacaaaacaa ggaaaatgta tatcgggtgg caacacttaa aatgcattgc
     7921 atatgtaaaa aaagagcagt attccttata ctttcatctt agaaaagaat tagtgtgtat
     7981 ggaagaaaac ggaggattaa gtgatggcaa atttagcaca gtcagtagag agaaaggcat
     8041 tcagcgtggc gattgatgca gcattgaagc atctgaataa ggatcgtgaa aaaggcctgc
     8101 ttcagattgt tgatcttgca gaaaagttca tgggcgataa cttccgtaag gaagcgtatg
     8161 acggagccag aaagatgatt caggatccgg acaataaatg gatgcattat gtaaatcgtc
     8221 ttctggatga gaccgatccc catgtagcaa agatgacagc attaaatctg ggatatcagg
     8281 cagcatttgt gggaacgaag acgattcgta agatgaggga agtacataac tgtaacatcc
     8341 cgtggctgat cttaatggat ccgaccagtg catgtaacct gcgctgcacc ggctgctggg
     8401 ctgctgagta cggacacaag ctcaatctta cgtttgatga gcttgacaat atcgtgacac
     8461 agggtaagga gcttggtatt tacttttata tgatgaccgg tggtgagccg ttagtcagaa
     8521 aagacgatat cattaagctc tgtgagaagc acaacgagtg tgcatttcac tgctacacaa
     8581 acggaactct cgtagaccag gcattctgtg acgagatgaa gcgtgtcggc aacctgtcct
     8641 tatccatcag tcttgaggga tttgaggagg ccaatgattt ccgccgcggc gaaggtgtat
     8701 ttgacaaggt tcttcatgca atggatctgc ttcatgagaa cgggctgatc ttcggtaaca
     8761 gcgtatgcta caccagcaag aacatggatg ccgttacatc ggatgagttc tttgatcttc
     8821 tgatcgagca cggaagccgt ttcgcatggt acttccatct gatgccggta ggtatgaagg
     8881 cttcaccgga actgatgccg accagagaac agagagaata tatttaccac agaa
//
LOCUS       DBMDQJ010000007         8880 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3467_length_8880_cov_1.575236, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000007 DBMDQJ010000000
VERSION     DBMDQJ010000007.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3467_length_8880_cov_1.575236"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(620..2008)
                     /locus_tag="ACZO9R_00285"
     CDS             complement(620..2008)
                     /locus_tag="ACZO9R_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0911540.1"
                     /translation="MGNTNTKPFGVKDKIGYMCGDIANDFTFIFASSYVMVFYTKVMG
                     VSAGMVGTMFLLARCLDAFTDIGMGRIVDNSKPTKNGRIRPWILRMCAFVALASFMMY
                     QSFLVDAPYAAKVAYMFITYILWGSIFYTSVNIPYGSMASVISSEPKDRSSLSVFRSL
                     GGTLAGILIGMLAPIVVYYTDEAGNKVVSASKISMIAGIFSVLAVICYLLCYTLTTER
                     VAYVPKPDVQKKTFGQTVSALFSSRSLMGLILSAVLLLLASLMSQGINQYLFADYFKN
                     TAALSVYSLLMLPCVLVLSAVSTKLAVKYGKKECSLIGMFISAGLYFIIGVLHVKNVW
                     LFVGLAFLAMIGMYFFQMQCWALVTDVIDDMEVKNHTRDDGTVYGMYSFARKIGQAIA
                     GGLSGWALGWIGYDELATSQTQEVANGIYNLSTFFPAIIYALCGLALLLIYPLSKAKV
                     EANVKELESRRK"
     gene            2200..3423
                     /locus_tag="ACZO9R_00290"
     CDS             2200..3423
                     /locus_tag="ACZO9R_00290"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR0911541.1"
                     /translation="MALPHISVEDCFPIVRQTILDTYKIDSFLMNYPYSDLERLDMGL
                     RKMVWGTYSTNSAIFSLSPEQDAWQIIILKSVLGFYNLIITVTNEEHPTMFGFMPFRT
                     EPATPPVISRIMRENGIPPQYTADMQQFYYTLPVVEMQSLITTLQHLITAFIPEFANS
                     HIEHVNYTSEQHKIDFNEERLHKFTSDYLAAIAEHLTLCGDAVASGNQALASERLKVL
                     LDSALSFTDTPLSPLRERLAYLNAFLATKLLDTQVHPSYIFNQLHTFQLRIYETTQMS
                     ELQHLPYEMVRKYCLLVKNFTYDNYSYLIRSVVNYINQHLSSELSLATLAAEFDKNAS
                     YLSNEFKKEVGDTLTTYINKHRILASLRYFNTTDMSVAEVSVAVGYTDMGYFSRLFRK
                     YVGQSPREYKKMLDK"
     gene            complement(3438..4475)
                     /locus_tag="ACZO9R_00295"
     CDS             complement(3438..4475)
                     /locus_tag="ACZO9R_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR0911542.1"
                     /translation="MKTKIYGTIGPACCDTEMLVHLFEKGMTGIRINLSHADLEEADA
                     WISGIHSAWSRLHDGTPEILIDLRGSELRIGTLGAERLLKEGDGLSLVAAGQNNLFDP
                     TEIPVPGTLLVALAPGQEILLDDGRIALKVEEQFRCGLSVAVECTVVRGGVLKSGKSI
                     AAPGVEIQTPTLTERDYRNLARVKQCRITGVMLPFVRNQEDLKNLKEALINEDAGDIR
                     IFAKIENLQGMEHLGELLPHCDEIVIARGDLGNAMPLAKLPCAQEEIAAQCRKHGRAF
                     MVVTQMLASMEHRAVPTRAEVSDIFRAVQQGAASVMLTGETAAGEYPLEAMEVLVDTV
                     REAEWYLEERR"
     gene            complement(4554..5495)
                     /locus_tag="ACZO9R_00300"
     CDS             complement(4554..5495)
                     /locus_tag="ACZO9R_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HIR0911543.1"
                     /translation="MMKYFMTDNQVIHEEEKLQDGVWVRMISPTQEECEEIADVLNVD
                     IDDIKAALDEEESSRIELQDGYTLILVDVPTTEIRHDKQSYTTIPLGIILTQDVIVTV
                     CTEDTPVLKNFVINRVKEFSTKKRLRFVYQILYRTATIYQTNLRIIDKRRTEIEERIG
                     QHTEDVDLIDLHELESTLVYFATSLRANGVVLDRLTRYKRLEQYPEDTELLGDVIVEN
                     QQAIEMTSIYRDIINGTRELMSSVIDNRLNNVMKYLTSITIVMAIPTVISGIYGMNVD
                     ERWMPFANTPHGFLLICVLTLLICIITMLILRKKKML"
     gene            complement(5517..6008)
                     /locus_tag="ACZO9R_00305"
     CDS             complement(5517..6008)
                     /locus_tag="ACZO9R_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR0911544.1"
                     /translation="MIRTAVPQDIPALMDIYNEAVLHTTATFDTEVKDMENRKAWFAE
                     HNGRYVIYVDVEDGKVAGYASLSRYRDRKAFDPAVEISIYVHKDYRGRGIGRSLMENV
                     LAYAAECDEIDTVISLITSENAASIHLHEVYGFEYCGQIKNAGTKFGRKLSLNAYQIL
                     YDR"
     gene            complement(6143..7045)
                     /locus_tag="ACZO9R_00310"
     CDS             complement(6143..7045)
                     /locus_tag="ACZO9R_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR0911545.1"
                     /translation="MNKFVMRQSLLLLLTATIWGVAFVAQSVGMDYIGPFTFNVVRNV
                     IGGLVLIPCIAVFHRTGAKEEDAGKTPGSRKNLLLGGICCGVMLFVAGNLQQIGIQYT
                     TVGKAGFITAMYIVLVPILSIFLGKKAGIKIWAAVALAVAGLYILCMTDGSFSLQKGD
                     LFVLLSAFAFSAHILVIDYFAPLADGVKMSCIQFFVCALLSAVCMWLFEKPDMGAVLQ
                     AWVPVLYAGVFSCGVAYTLQIVGQRGMNPTVASLILSLESVISLIAGWVILGQALSVR
                     ELSGCVLMFAAIILAQLPERKRGA"
     gene            complement(7053..7226)
                     /locus_tag="ACZO9R_00315"
     CDS             complement(7053..7226)
                     /locus_tag="ACZO9R_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911546.1"
                     /translation="MEKGRTEALAKERIGKVGLADKVVFMADGRILEEGKPEEIFSNP
                     KMPEARQFLRSVL"
     gene            complement(7261..7908)
                     /locus_tag="ACZO9R_00320"
     CDS             complement(7261..7908)
                     /locus_tag="ACZO9R_00320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0911547.1"
                     /translation="MKGAIFDMDGTLLDSMQVWVHVGEQYLRNRGIEPEEGLGDVLFP
                     MSMRDGAVYVKERYGLPDPPDTIVTDMDAIVFAAYRDEVLPKPGVAAYLESLKKQGIP
                     MAVATATNRPMVEAALARTGLAGYFKQIFTCTEIGAGKERPDIYLAAAKAFGTHPADT
                     WVFEDALYAIKTAKAAGFYTVGLYDETSRNDQEAIAGLADCYVREIQSVPTQDCT"
     gene            complement(7905..8552)
                     /gene="thiE"
                     /locus_tag="ACZO9R_00325"
     CDS             complement(7905..8552)
                     /gene="thiE"
                     /locus_tag="ACZO9R_00325"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR0911548.1"
                     /translation="MKCDKRDLLLYAVTDRSWSEGTTLGAQVEEALRGGATFVQLREK
                     KLTGEALRAEALEIQAVCRRYGVPFVINDDVALAKEIDADGVHVGQDDMEAGDVRAIL
                     GADKIIGVSAHNVEEALRAERHGADYLGVGAAFTTSSKPEAGHIDAETIRAICETVKI
                     PVIAIGGIKEENLLQLRGNGLCGVAVISAIFAQKDIEAATRRLHALAEEMAGDQE"
     gene            complement(8539..>8880)
                     /locus_tag="ACZO9R_00330"
     CDS             complement(8539..>8880)
                     /locus_tag="ACZO9R_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR0911549.1"
                     /translation="QCVVIRGGRVEMSRVTGTGCQLSGLAAAFLAVARNMEAPAGSTV
                     GNTAVGAACAARGMEAVAAAVCVMNAAGEIAWEQRGLCGGNASYGTGIIDAVYRMDQK
                     MLEGRIHYEVR"
BASE COUNT         2112 a         2661 c         1875 g         2232 t
ORIGIN      
        1 aaaggggcag tcgccccttt tcgtgaagct ggattttgat aagtagcatt ataagaaact
       61 cgcactttgc gatttccgtt ggaaatgcag atggaaattc ggtcaaaaaa tatggcattt
      121 ctaaccagcc agatgtttgc atcttcgatg atccgtgtct ttccaagttt ggaacaatcc
      181 gcgggtcagg tccgcacccc gtgagacaat ttgtagggtt ttcgtgctgc acttacctgc
      241 actcccgcag taaaacataa ccgcgcgaag cagatgtttg agttgccatc tgcccggact
      301 ttcatagcag gtggcaacga gtttctgttc gcgcggtttt atgatgtcta cgagggagtg
      361 cagttagtgc agctagaaag cccgcccatt gtctccggtg tgccggacat gcacccgtcg
      421 gatagtcaaa acgtggaaga gacacggaat ctgatgcaaa catccaactg gtagaaatgc
      481 tcacattttt ctccaacgaa tattccatct gcatttccaa cggaaatctg catatgtgct
      541 tcttataatg ctgcttatca catcaactgc acgaaaaagg ggctgcacgc ttaatgtgca
      601 gccccttaac aagcctatat tattttcttc tggactcaag ttcttttacg tttgcctcta
      661 ccttcgcctt ggaaagcgga tatataagta acagtgctag tccgcataat gcatagatga
      721 ttgccgggaa gaaggtggac agattgtaga ttccatttgc aacttcctgc gtctggcttg
      781 ttgccagctc atcataaccg atccagccaa gtgcccatcc ggaaagtccg cccgcaattg
      841 cctgaccgat ctttcttgcg aaagagtaca taccgtatac ggtaccatca tctctggtgt
      901 gattcttcac ttccatgtca tcgataacgt ccgttaccag tgcccagcac tgcatctgga
      961 agaaatacat accgatcatg gcaaggaatg ccagtccgac gaacagccat acattcttta
     1021 catgcagtac gccaataata aaatataatc ctgcggagat aaacattccg ataagactgc
     1081 actctttctt tccgtacttc acggcaagct ttgtggacac tgcggaaagt acaagcacgc
     1141 acggaagcat cagcagcgaa tatacggata atgctgcagt gttcttaaag taatctgcaa
     1201 acagatactg gttgatgccc tgggacatta aagatgccaa caacaacagc actgccgaga
     1261 gaatcaggcc catcagcgat ctgctggaaa acagtgcgga aaccgtctgt ccgaacgtct
     1321 tcttctggac atccggcttc ggtacatacg cgacacgttc tgttgtcagc gtatagcata
     1381 acagatagca gataacggca agtacggaaa agattcctgc gatcatggaa attttggacg
     1441 cggatacgac cttgtttcct gcctcatcgg tgtaatatac cactatcggt gcaagcatac
     1501 cgatcaggat acctgccagc gtaccgccca gagaacggaa cacagataag gagcttcggt
     1561 ccttcggctc gctggaaata acagatgcca tggatccata tggaatattt acggatgtat
     1621 agaaaatgga tccccataaa atgtaagtga tgaacatgta agcaactttt gccgcatacg
     1681 gtgcatctac cagaaatgac tggtacatca tgaacgatgc cagtgcaaca aatgcgcaca
     1741 tacgcagaat ccacggacgg atgcgtccgt tcttggtcgg cttgctgtta tcaacaatgc
     1801 gtcccattcc gatgtcggta aatgcatcca gacatcttgc aagcaggaac atggtaccta
     1861 ccataccggc agagactccc atgacctttg tgtaaaatac cattacatag ctgctggcga
     1921 aaatgaatgt aaaatcattc gcaatatcac cgcacatgta accgattttg tcctttacgc
     1981 caaacggctt ggtgtttgtg tttcccataa tgcgcctcct gtagcagacc gccccatgcg
     2041 gtctctttga tggttttatt atagcaactt ccctttccct gtaccttgcc tttttttgtt
     2101 tgacatggat tatttttgtg ttatatttgt tttgtactaa aattgtttca cgttgttatt
     2161 tacagctaac agcttctgga ttaagcaagg aggtttctca tggcattgcc ccatatttcc
     2221 gtggaggatt gtttcccgat cgtccgccag accattttag atacttacaa aatcgattct
     2281 ttccttatga attatccgta ttccgatctc gaacggctcg acatgggtct taggaagatg
     2341 gtctggggaa cctactccac aaactccgcc attttctctc tgtcaccaga acaggatgca
     2401 tggcagatca ttatattaaa aagtgttctg ggcttctaca atctgattat caccgtcaca
     2461 aatgaggaac atcctaccat gttcggtttt atgccgttcc gtaccgaacc cgctaccccg
     2521 cctgtgatca gccgcatcat gcgcgaaaac ggcatcccgc cgcagtacac cgccgacatg
     2581 cagcaattct attacacgct gcccgttgtg gaaatgcaga gcctgattac aacactgcag
     2641 catctgatta ccgcttttat cccagaattt gcaaacagcc acattgagca tgtcaattat
     2701 acctccgaac agcataagat agattttaat gaggaacggc ttcataaatt tacctctgat
     2761 tatctggcag ccatagccga acacttaacg ctctgcggag acgcggttgc ttccgggaat
     2821 caggcgctcg cttctgaacg tttaaaagtc cttctggatt ccgctttatc cttcaccgat
     2881 acaccgcttt ccccgctcag ggaacgcctt gcctacctca atgcgttttt agccacaaag
     2941 ctgctggata cacaggtgca cccctcttat atcttcaatc agctgcacac ctttcagctt
     3001 cggatttatg agaccaccca gatgtccgag ctgcagcatc ttccttatga aatggtacgc
     3061 aaatactgcc tgctcgtgaa aaactttacc tatgacaatt attcctatct gatacgaagc
     3121 gtggtcaatt atatcaatca gcacctgtcc tcggaattat cgctcgccac gctcgccgcg
     3181 gagtttgaca aaaatgcttc ctatctctcc aacgaattta aaaaggaagt cggcgacaca
     3241 ctcacaactt atatcaacaa gcaccgcatt ctcgcctcac tgcgttactt taacaccaca
     3301 gacatgagtg tcgcggaagt atccgtcgcc gtcggttaca ccgatatggg ctatttctcc
     3361 cgcctcttcc ggaaatatgt aggacagagt cctcgcgaat ataaaaagat gctggataaa
     3421 tgacatttta tgccccatta tctgcgctct tccagatacc actccgcctc ccgcaccgta
     3481 tccaccagaa cctccatcgc ctccagcgga tattctcccg cagcggtctc gcctgtcagc
     3541 atcacagatg ccgcgccctg ctgcaccgcg cggaatatat cggacacttc cgcccgcgtc
     3601 ggcaccgccc gatgctccat ggaggcgagc atctgcgtga caaccataaa cgcgcgcccg
     3661 tgctttctac actgagccgc aatctcctcc tgcgcgcacg gaagctttgc cagaggcatc
     3721 gcattcccca gatctcctct ggcgatcacg atctcatcgc agtgtggcaa cagctccccc
     3781 agatgctcca ttccctgcag attctcaatc tttgcgaaaa tgcggatgtc cccggcatcc
     3841 tcatttatta aagcttcttt taagttcttt aagtcttctt gatttctcac gaatggaagc
     3901 atgacccctg tgatcctgca ctgctttact ctggcaagat tcctgtaatc acgctccgtc
     3961 agcgtcggtg tctgtatctc cacaccgggc gccgcgatac ttttcccgct ttttaagacg
     4021 ccgccccgca caacggtaca ttccaccgcg acagacaatc cgcagcggaa ttgttcttct
     4081 acttttaatg caatcctgcc gtcgtccagc aaaatctcct gtccgggcgc aagcgccaca
     4141 agcagcgttc ccggcaccgg aatctccgtc ggatcaaaca gattgttttg tccggccgcc
     4201 acaaggctca gaccgtctcc ctcttttaaa agacgctctg ccccaagcgt accgatgcgg
     4261 agctccgatc cccgcaggtc aatcagaatc tctggcgttc cgtcatgcag ccggctccat
     4321 gcgctgtgga tgccggagat ccacgcatcc gcttcttcca gatccgcatg ggacagattg
     4381 attctgattc ctgtcattcc tttttcaaac aaatgaacca gcatctctgt atcacagcat
     4441 gctggtccga tggttccgta aatttttgtc ttcattatac tcttctctcc gcgcagctgc
     4501 tctgctgcgc cgcccgtgat tcgatcgtgc ggcagattct cccgccgctt gttctatagc
     4561 atctttttct tacgcagtat cagcatcgtg ataatgcaga tcaacagagt cagcacacag
     4621 atcagcaaaa atccgtgcgg cgtattagca aacggcatcc accgctcatc cacgttcatt
     4681 ccatagatac cggaaatgac cgtcggaatc gccatgacaa tcgtgattga ggtcagatac
     4741 ttcatgacgt tgttcaggcg gttgtcaatc acggaggaca tcagttctct ggtgccgttg
     4801 atgatgtcac ggtagatgga agtcatctcg atcgcctgct ggttctcgac tatgacatcc
     4861 cccagaagct ccgtatcctc cgggtactgc tccagtctct tatagcgcgt cagccggtct
     4921 aacaccacgc cgtttgcacg cagggatgtg gcgaaataga ccagcgtgga ctccagttcg
     4981 tgcagatcaa tcagatccac gtcctcggtg tgttgtccga tccgctcctc aatctccgtg
     5041 cggcgcttgt caatgatgcg caggttggtc tgatagattg tcgcggtgcg gtacagaatc
     5101 tggtacacaa agcgcagacg ctttttcgtg ctgaactcct tcacgcggtt gatcacaaaa
     5161 ttcttgagca ccggcgtatc ctccgtgcac accgtcacga tcacatcctg cgttaagata
     5221 atgccgagcg gaattgtcgt gtaggactgc ttatcgtgcc ggatctccgt cgtcggaacg
     5281 tccaccagaa tcagcgtata tccgtcctgc agttcgatac gggagctttc ctcctcatcg
     5341 agtgctgcct tgatatcgtc gatatccaca ttcagcacat cggcaatctc ctcacattcc
     5401 tcctgcgtgg ggctgatcat ccgcacccac acgccgtcct gcagcttctc ctcctcatgt
     5461 atcacctgat tatcggtcat aaaatatttc atcatagctt ttgccctata tcctctctac
     5521 ctgtcatata gaatctgata tgcattcaga ctgagttttc ttccaaactt tgtccctgca
     5581 tttttaatct gaccgcaata ctcaaatcca tacacctcat gcagatgaat gctcgccgca
     5641 ttctcactgg taatcaaaga gatgaccgtg tcaatctcgt cacactccgc tgcataggca
     5701 agcacgttct ccatcaggct gcggccgatg ccacgccctc gataatcctt gtgtacataa
     5761 atcgatatct ccaccgccgg gtcaaatgcc ttacggtccc tgtaacggga aagcgacgca
     5821 tatcccgcca ccttgccgtc ctccacatca acgtaaatga catagcgccc gttatgctcc
     5881 gcaaaccatg ccttccggtt ctccatatcc ttaacttccg tatcaaacgt cgccgtcgtg
     5941 tgaaggaccg cttcattata tatatccatc aatgcaggaa tatcctgcgg tactgctgtg
     6001 cgtatcattg tcttaccagc tttcgtgtcc taaaatgtct ctgtaacaga aaacgccttc
     6061 ggaatccgaa ggcgcctgcc cttttttatc atatcacagt ttttctggca cttctacgct
     6121 aaatttgcaa aatcacagga aatcacgcgc cacgtttccg ctccggaagc tgcgccagaa
     6181 tgattgcagc aaacatcagc acacagccgg aaagctcacg caccgagagc gcctgcccta
     6241 atattaccca gcctgcaatc aatgaaatta ccgactccag gctgaggatt aaggacgcca
     6301 ccgttgggtt catgccacgc tgtccgacaa tctggagtgt gtacgccacg ccacaggaaa
     6361 aaacacctgc gtacagcaca ggaacccacg cctgcagaac cgctcccata tccggtttct
     6421 caaacagcca catgcagacc gccgacagca gtgcacacac aaaaaactgg atgcacgaca
     6481 tttttacacc gtccgccagc ggcgcaaaat aatcaataac aagaatgtgc gccgaaaatg
     6541 caaatgccga cagcagaacg aacagatctc ccttctgcag ggaaaagctg ccatctgtca
     6601 tacataaaat gtataacccc gcgacagcca gcgccacagc ggcccagatt ttgattcccg
     6661 ccttttttcc taaaaaaata cttaatatcg gcaccagcac aatgtacatc gctgtgataa
     6721 atcccgcctt gcctactgtt gtatactgta ttccgatctg ttgcagattg cctgccacaa
     6781 aaagcatgac cccgcagcag attccgccaa gcagcagatt tttccggctt cccggtgtct
     6841 ttccggcatc ctcttccttt gcacctgttc tatgaaacac tgcaatacat gggatcagca
     6901 ctaacccgcc aatcacattc cgcactacgt taaaggtaaa cggtccgata taatccattc
     6961 caacactctg cgccacaaac gcaacgcccc agatcgttgc cgtcaaaagc agcagtaaag
     7021 attgtctcat cacaaattta ttcatcaccc tactacagaa cgctccgcag gaactgtctt
     7081 gcctctggca tcttcggatt ggaaaaaatc tcctccggct ttccttcctc aagaattctt
     7141 ccatccgcca taaagacaac cttgtccgca agtccgactt ttccgatccg ctcttttgca
     7201 agagcttctg tccttccttt ctccatcttt tttacatgaa caggcgccag cattacattc
     7261 tcaagtacag tcttgtgtgg gaacagattg aatctcccgc acatagcagt ccgccaggcc
     7321 ggcaatcgcc tcctgatcat ttctgctcgt ctcatcgtag agccccaccg tataaaaccc
     7381 ggctgccttt gccgtcttaa tcgcatagag cgcatcctcg aacacccagg tgtccgccgg
     7441 gtgcgtacca aatgcctttg ccgctgccag atagatgtcc ggcctttctt ttcccgcgcc
     7501 gatctccgtg caggtaaaaa tctgtttaaa atagccggca agtcctgtcc gcgcgagcgc
     7561 cgcctccacc atcggccggt tcgtcgcggt ggcgaccgcc atcggtattc cctgcttttt
     7621 gagcgattcc agatatgccg caacgcccgg cttcggcaga acctcatccc ggtatgccgc
     7681 aaacacaatc gcgtccatgt ccgtcacaat cgtatcaggg ggatccggca aaccgtacct
     7741 ttccttcaca tacacagctc cgtcccgcat gctcatggga aaaagcacat ctccgagccc
     7801 ttcttccggt tcgattcccc ggttccgcag atactgctcg ccgacgtgaa cccacacctg
     7861 catggagtct agcagcgtgc cgtccatatc aaaaattgca cccttcattc ctgatccccc
     7921 gccatttcct ctgccagagc gtgcagacgc ctcgtcgccg cctcgatatc cttctgtgca
     7981 aaaatggcgc tgatcacggc aacgccgcac agaccgtttc cgcgcagctg cagaagattc
     8041 tcctccttga tgccgccgat cgcaatgacc ggaatcttta ccgtctcgca gatggcacgt
     8101 atcgtctccg cgtcaatgtg tcccgcctcc ggtttggagc tggtcgtaaa cgcagcgccg
     8161 acgcccagat aatccgcacc atgacgttct gcgcggagcg cttcttccac attatgcgcg
     8221 gatacgccga tgattttgtc agcgcccaga atggcacgca catcgccggc ttccatatca
     8281 tcctgcccga catgcacccc gtccgcgtca atctctttcg caagcgccac gtcgtcgtta
     8341 atcacaaacg gcacgccgta ccgccggcac actgcctgga tctccaacgc ctccgcacgg
     8401 agtgcttccc cggtcagctt tttttcccgt agctggacaa atgtcgcgcc tccccggagc
     8461 gcttcttcca cctgcgcccc cagcgttgtt ccttcgctcc agcttcgatc agtcactgca
     8521 taaagcagca gatctctttt atcgcacttc ataatgaatc cttccctcca gcattttctg
     8581 gtccatccgg tacacagcat caatgatacc ggtcccgtag gaggcatttc cgccgcataa
     8641 accgcgctgc tcccacgcaa tctctcctgc tgcattcatc acgcagactg ccgccgcgac
     8701 cgcctccatg ccgcgcgccg cgcaagctgc tcctaccgca gtattcccga cggtgcttcc
     8761 agctggcgct tccatattcc gtgccaccgc gaggaacgcc gccgcaagtc cggaaagctg
     8821 gcacccggtt ccggtcaccc ggctcatttc cactctccct ccgcggatca ccacacactg
//
LOCUS       DBMDQJ010000008         8466 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3659_length_8466_cov_1.629767, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000008 DBMDQJ010000000
VERSION     DBMDQJ010000008.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 8466)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8466)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8466
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3659_length_8466_cov_1.629767"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..990)
                     /locus_tag="ACZO9R_00335"
     CDS             complement(<1..990)
                     /locus_tag="ACZO9R_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0911550.1"
                     /translation="MPVFKAFFAIAKKRRNAALIYLGVYAVITLLLSALSQDSINENF
                     KASQRSIAITDEDNTDASRALCDYLASIHQVTTPEPGEDTALLDAIYYRTLNYAMVIP
                     EGFEDRVLAGETKDLVSSLTIPGSSSSQYVDNQITQYVQALQLYLAGGDTIAEAIDHT
                     DHTISDLPKVSVTSFQMSDADTNSMVFYFFQYLPYIFLAILFAGMAPILVTLNSRDMK
                     ARTACSALPGRTRTLAMASGCLLYSLGIWLLFLLLGLVFYRKAMLQPYVGYAILNSLA
                     FLLFTTALSLLISTFSPDDNVLNMLANIVGLSMSFLCGVFVPQSMLSGSVLAVG"
     gene            complement(977..2173)
                     /locus_tag="ACZO9R_00340"
     CDS             complement(977..2173)
                     /locus_tag="ACZO9R_00340"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0911551.1"
                     /translation="MFFHTMKYTLLNLLRDKGQVFWCVAFPLLLGTMFHFAFGGLGSG
                     ESFSAIPVAVVMQEESSELRSMFDSLSKPGEDQFLEVTYASAADAKELLKAKEVYGII
                     TADLPTVSDYRNAASAKDVSTYPLSLTLSAEMTSDPLYQSILSVLVDRFNIEYSAIAQ
                     AAMKDPTKLPALLDAVSTEVSYIRHESLGAGSVDESLTYFFNLIAMTCLYAAMIGNTL
                     AISNQANLSDLGARRNVSPVPRLLAALGDLTGALIFEFSSLLITIAYLTLVLKVDFGN
                     HLGGVALTCLCGCLAGLSLGFLVGCIGQFSKETKFGFLMAAIMICCFLSGLMIGNMRA
                     LVEDFCPLLNRINPAALISDALYSLVVYPSYTRFFTNIAGLLLLSALFCLGGLALVRR
                     KKYASL"
     gene            complement(2185..3126)
                     /locus_tag="ACZO9R_00345"
     CDS             complement(2185..3126)
                     /locus_tag="ACZO9R_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911552.1"
                     /translation="MSVPVVEIENLVKRYQELVALDHFNLTIEEGEIFGLLGPNGSGK
                     TTTINCILALLAFDKGTVRVFGQEMRPDSYDLKRQIGVVMQNVAVFQELTVYENIDYF
                     CGLYIRDKALRRQYVEDAIDFVGLNDFRKFYPKKLSGGLLRRLNIACGIAHKPKLIIL
                     DEPTVAVDPQSRNKILEGIQELNRNGATIIYTSHYMEEVEQICTRIAIMDKGKNLAIG
                     TTEELKRMIKKSEIISIDILDLLPAQIAAIRDIKHVYEVTFEHHCLKVLFSGGSHNLP
                     HILNYLQEQNVSFGRVYSELPTLNDVFLEITGKELRD"
     gene            3351..4157
                     /locus_tag="ACZO9R_00350"
     CDS             3351..4157
                     /locus_tag="ACZO9R_00350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019350.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911553.1"
                     /translation="MKRIVDKGLLLAGGLLMAGQSGRLAAPVIALLLAMTAAAYGSCV
                     DNRRWHCVCLAGMFAVCFILPELCFFVPVLLYDCAEKKEMRLWFLSVPGLAFFYREQI
                     IRQPFLWAADGMLIVAAILLACRTGRILYLEQEMIRLRDTSTELNLVLQEKNKNLMEK
                     QDYEIYLATLRERNRIAREIHDNVGHMLSRSILQMGALITIHKEEPLHGQLAGVGETL
                     NQAMNSIRESVHDLHDESIDLRQSIAEATREMKEHSSRLITICLRRFHAM"
     gene            4130..4471
                     /locus_tag="ACZO9R_00355"
     CDS             4130..4471
                     /locus_tag="ACZO9R_00355"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911554.1"
                     /translation="MSPEIPRNVKYCFITAVKEAMSNIAKHSDADKISVILREHPAFY
                     QMTVEDNGTVGRKGQSGPLWEEEVVRMDGKEGIGLSNMKERVQALGGTFRIHTEKGFV
                     IFISIPKRDKM"
     gene            4502..5125
                     /locus_tag="ACZO9R_00360"
     CDS             4502..5125
                     /locus_tag="ACZO9R_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0911555.1"
                     /translation="MRVLVVDDDKLVAVSLKTILEADGEITVVGLGEAGDDAIRLYRE
                     QHPDILLMDIRMQGMSGLDAAECILKEDQAAKILFLTTFSDDEYIVKALNMGAKGYLL
                     KQDFESIVPALKAVYGGQSVFGGQIMGKIPVLLAGKGSFSYHDYDISEKEQGIIELVA
                     EGLSNREIAEKLYLSEGTVRNYISTILDKLKLRDRTQLAVFYYKNCG"
     gene            5217..5993
                     /locus_tag="ACZO9R_00365"
     CDS             5217..5993
                     /locus_tag="ACZO9R_00365"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR0911556.1"
                     /translation="MIFETHAHYDDEVFNEDREQLLAGLPEKGIGRVINVGASIETTR
                     TTLALAEAYDYIYAAVGVHPSDIDGLNEETYSWLKAQTAHPKTVAVGEIGLDYYWDKE
                     PEVQAAQREWFVRQLALAGETDLPVIIHSRDAAEDTMRILKETGRFREHPGVIHCYSY
                     SPEQAKEYVKMGYCIGVGGVVTFKNAKKLRQTVEQISLEHILLETDCPYMAPEPHRGT
                     RNDSSNLSYVAARIAEIKGVSVQEVEQTTWENACRVFGVR"
     gene            complement(6007..6795)
                     /locus_tag="ACZO9R_00370"
     CDS             complement(6007..6795)
                     /locus_tag="ACZO9R_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03915 family putative DNA repair protein"
                     /protein_id="HIR0911557.1"
                     /translation="MYVFICEDSLEGIFTGVYDACASRLGHRNIRLATGEPENYELFS
                     EYIHVTPSADKTGKVIRTITSRFGMQFYESIYQAAMSGEPSSGRKMDKADAIYETILL
                     ALASGDGQKVLLSLGEPCVYRIFELCRATNREACHHLEFLRFSELENGVLFATIHPKD
                     NVLPYLAEHFTDRLPSENFMIYDATHQSVAVHKALSSYILTDAADLDLEKVSRYSSDE
                     EAFRQLWLTFFDHIAIESRKNPGLQMQLIPKRYWADTPEYSRFC"
     gene            complement(6836..8131)
                     /locus_tag="ACZO9R_00375"
     CDS             complement(6836..8131)
                     /locus_tag="ACZO9R_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA modification/repair radical SAM
                     protein"
                     /protein_id="HIR0911558.1"
                     /translation="MNIQQNLSLMEKLAILTDAAKYDVACTSSGADRRGDGRHMGNCL
                     APGVCHAFAADGRCISLLKILFTNECIFNCAYCQNNCNSDVPRASFTPDEICTLTMEF
                     YRRNYIEGLFLSSGILISPNYTMDLIYQTLYRLRTVHHFNGYIHVKAIPGADPALIEK
                     AGFLADRMSINLELPTANGLKQLAPCKSRHTILSPMRQIQNGITENQNELMVYRKAPK
                     FVPAGQSTQMIIGATPENDYQIMRVSEALYEKFHLKRVFYSAFINVNGDSSLPVLPGG
                     PPLLREHRLYQADWLLRYYRFHVDELLSEDRPNFNIYLDPKCDWALRHLDTFPVEINR
                     ASYQTLLRVPGIGYKSAQRIVAARRDTALTYDDLKKIGVVLKRALYFITCSGRMMYRT
                     KLDEDYICSHLMADHTQVPTELRNSGYQQLSLFDTGLAL"
BASE COUNT         2170 a         1892 c         2488 g         1916 t
ORIGIN      
        1 tcctacggca agtacggaac cggagagcat ggactgcggg acaaagacac cgcagagaaa
       61 ggacatggac agaccgacga tatttgccag catatttaat acattgtcat ccggcgagaa
      121 cgtcgagatg agcagggaga gtgcagtcgt aaaaagcaga aaggcaaggc tgttcaagat
      181 ggcatagccg acatagggct gcagcatggc tttccggtag aatacgagac caagcagcag
      241 aaagagcagc cagatgccga gactgtacag gagacagccg gatgccatgg caagagtacg
      301 tgttcttccg ggaagagcgg agcacgccgt ccgggctttc atgtcgcggc tgtttaaggt
      361 aaccagaatc ggagccatgc cggcgaacag gattgccaga aagatatacg gaagatactg
      421 gaagaaataa aaaaccatgc tgttagtgtc ggcgtcggac atctgaaaag atgtgacgga
      481 gactttggga aggtcggaaa tggtatgatc cgtatggtcg atggcctcgg caatggtgtc
      541 accgcccgcc aggtagagct gcagtgcctg cacatactgc gtaatctggt tgtccacgta
      601 ctggctgctg ctgcttcccg gaattgtcag ggaggagaca aggtctttgg tctcgccggc
      661 gagcacccgg tcttcaaatc cttccgggat gaccatggca tagttgagcg tgcggtagta
      721 gattgcatct aacagtgcgg tgtcctctcc aggctccggt gttgttacct ggtggatgga
      781 tgcgaggtaa tcgcacagag cgcggcttgc atccgtgttg tcttcatctg taatggcgat
      841 agagcgctgg ctggctttga agttctcgtt gatggaatcc tgcgagagtg ccgacagcag
      901 cagagtgatc accgcgtaga cgcccaaata gatcaatgcg gcatttcgcc gtttttttgc
      961 gattgcgaaa aatgctttaa agactggcat attttttcct ccttaccagt gcaagaccgc
     1021 cgagacagaa aagtgccgag agtaacagga gtcctgcgat atttgtaaaa aaccgtgtgt
     1081 aggacgggta cacgacgagt gaatacagcg catcggagat gagagctgca ggattgatgc
     1141 ggttgagcag cgggcagaaa tcttccacaa gtgcacgcat gtttccgatc atcagaccgc
     1201 ttaaaaagca gcagatcata attgcagcca tcaggaaacc aaacttggtc tctttggaaa
     1261 actgcccgat acaaccgacc agaaatccca gactcagccc ggcaaggcaa ccgcagaggc
     1321 aggtcagcgc cacaccgccg agatggtttc cgaaatcaac cttaagaacc agtgtcagat
     1381 aggcaatggt gatcagcaag gaggaaaact caaaaatcag agctccggtc agatcaccga
     1441 gggcggcaag cagccgggga accggcgaga cattgcgtct ggcacccaga tctgacagat
     1501 ttgcctgatt ggagatggcg agcgtgttgc cgatcatggc agcatagagg caggtcatgg
     1561 caatcagatt aaaaaagtaa gtaagggatt catccacgga gccggcacca agggattcgt
     1621 gccggatata gctgacttcc gtagaaaccg catcaagcag tgccggaagc ttcgtgggat
     1681 ctttcattgc ggcctgggcg atggcactgt attctatgtt gaaacgatcc acaagcacac
     1741 ttaaaatact ctggtacaga ggatcggaag tcatctctgc ggagagtgtc agcgatagag
     1801 gatacgtgga aacatccttg gcggatgcgg catttctata atcggaaacg gttggcagat
     1861 cagcggtaat gatgccgtag acttcttttg ctttcagcag ttccttggca tccgcagcgg
     1921 aagcataggt aacttcaaga aactgatctt cgccgggttt ggacagggaa tcaaacatgg
     1981 agcgcagctc ggaggattcc tcttgcatca caactgcaac cgggatggcg gaaaaacttt
     2041 cgccactccc cagtccgccg aatgcaaaat ggaacatcgt tccgagcaga agtggaaaag
     2101 cgacacacca gaatacctga cctttatccc gcagcagatt aagcagtgta tatttcattg
     2161 tatgaaaaaa catggtggta cctcctaatc acgaagttct tttccggtaa tttccagaaa
     2221 gacgtcattt aaggtcggaa gttcggaata gactcttccg aaagacacat tctgttcctg
     2281 cagatagttc agaatgtgcg gcagattgtg ggaaccgccg ctgaacagca cttttaagca
     2341 gtgatgttca aaggtcacct catagacgtg ctttatgtca cggatcgctg caatctgcgc
     2401 aggaagaaga tccagaatat caatcgagat gatctcgctc tttttgatca ttcgttttaa
     2461 ctcttccgtg gttccaatgg caagattttt gcctttgtcc ataatggcga tccgggtaca
     2521 gatctgctcc acctcctcca tatagtggga ggtataaatg atcgtggcac cattgcggtt
     2581 taactcctgg attccctcca gaattttatt gcggctctgt gggtcaaccg ccacagtcgg
     2641 ctcgtcgaga atgatgagct ttggcttgtg ggcaattccg cacgcgatat tcagacggcg
     2701 cagcagacca ccggaaagct ttttcggata aaatttgcgg aaatcgttga gaccgacaaa
     2761 atcgatggca tcttccacat actggcgccg gagcgctttg tcgcggatat acagaccaca
     2821 gaaataatca atgttctcat agacggtcaa ttcctgaaag acagcgacat tttgcatgac
     2881 aacgccgatc tgacgtttca gatcgtagct gtccggccgc atttcctgac cgaagacccg
     2941 gacggttccc ttgtcaaagg caagcagtgc aaggatgcag ttgatggttg tcgtctttcc
     3001 ggagccgttt ggtccaagca gaccgaagat ctcaccttcc tcaattgtga gattgaagtg
     3061 atcgagggcg acaagctcct gatagcgttt taccagattt tctatttcta caacaggtac
     3121 tgacataaca aatcctcctc atttgatggt aattacagta tagcgggtgg cgcccggcgg
     3181 taccagtgag cagcgtcaga tgtttgatgt gacaaatgtc ataaatctct tatttttcaa
     3241 aaaagatgtg gcggcagccc tgtaatgatg atatggttat cataacgtgt gcgggaaagt
     3301 ggaagatatc ccggaaatat ggcggcatat gtggacaggg agagggtaga atgaagcgga
     3361 ttgtggataa aggactgttg cttgccggcg gtctgcttat ggcgggacag tcgggaaggc
     3421 ttgcagcgcc ggtcatagcg ttgctattgg cgatgactgc ggcggcatat ggcagctgtg
     3481 tggacaacag acgatggcat tgtgtgtgcc ttgcggggat gtttgcggtc tgctttatcc
     3541 tgccggaact ctgttttttc gtgccggttc ttctgtatga ctgtgcggaa aaaaaggaga
     3601 tgcggctctg gttcttgagt gttccggggc tcgctttttt ctatcgggaa cagattatcc
     3661 ggcagccctt tttgtgggcg gcggacggta tgctgatcgt ggctgcgata ttgctggcgt
     3721 gcagaacggg cagaattctg tatctggaac aggagatgat ccgtcttcgg gataccagca
     3781 cggaattgaa tctggtgctg caggaaaaaa acaagaacct gatggaaaaa caggattatg
     3841 agatctacct tgcgacactg cgggagcgca accgcattgc aagagagatt catgataatg
     3901 tggggcatat gctttcaaga agtattctgc agatgggggc actgattacg attcataagg
     3961 aggagccgct gcacggacag ctcgccgggg tgggggagac gttaaatcag gcgatgaaca
     4021 gtatccgtga gagcgtgcac gatctgcacg atgagtccat tgatctgcgg cagtccattg
     4081 cggaggcgac ccgtgagatg aaggagcaca gctcacggtt gattacgata tgtctccgga
     4141 gattccacgc aatgtaaagt actgctttat cacagcggta aaagaagcca tgtcgaatat
     4201 tgcaaaacac tccgatgcgg ataagatctc cgtgattttg cgggagcacc cggcgttcta
     4261 tcagatgacc gttgaggaca acggaacggt gggacgaaag ggacagagcg gaccattgtg
     4321 ggaggaagag gtcgtccgta tggacggaaa ggaagggatt ggtctttcca atatgaagga
     4381 gcgcgtgcag gcactgggcg gaaccttccg gattcatacg gaaaagggat ttgtgatctt
     4441 tatttcaatt cccaagaggg ataaaatgta gatacggtaa tgatgactgg gagagagaaa
     4501 aatgagagtt ctggtggttg acgatgataa gctggtggca gtatccctaa aaacgattct
     4561 ggaagctgac ggggagatta cggtcgtggg tcttggagag gcgggagatg acgccatccg
     4621 gctttatcgg gagcagcatc ctgatattct gctgatggat atccgcatgc agggaatgtc
     4681 cggattggat gcagctgaat gtatcttaaa agaagatcaa gcggcaaaaa ttttgttttt
     4741 gacaaccttt tccgatgacg aatacattgt aaaggcactg aatatgggag cgaaggggta
     4801 tctgctgaaa caggactttg agtcgatcgt tccggcactc aaggctgtct acggcgggca
     4861 gagtgtattc ggcggacaga ttatgggaaa aattccggtg cttctggcag gaaaaggcag
     4921 cttttcctat catgattacg acatcagtga gaaggagcag ggcattattg aactggtggc
     4981 agaaggctta agcaaccggg agattgcaga aaaattgtat ttgagcgagg gtacggtgcg
     5041 caattatatc agcaccatac tggataaatt aaagttacga gaccgcacac agctggcagt
     5101 cttttattat aagaactgcg gctgagccgg cggtacagca atcgcacaga agctgtgccg
     5161 acagacagcg acatgaaaga gacggcaatg cagcaacagg gaagatgagg aaaacaatga
     5221 tatttgagac acatgcacat tatgatgatg aagtatttaa tgaggataga gagcaattat
     5281 tagctgggct cccggaaaag ggaatcggga gggtgattaa cgtcggtgcg tccattgaga
     5341 cgacgaggac gacgctcgcg ttggcggagg cgtatgatta catctatgcc gccgtgggtg
     5401 tgcatccgag cgatattgac gggctgaacg aggagacata ttcctggctc aaagcgcaga
     5461 cggcacatcc gaagacggta gcggtgggag agattggtct ggattattac tgggacaagg
     5521 agccggaagt acaggcagcg cagcgggaat ggttcgtgcg ccagcttgca cttgccgggg
     5581 agacggatct cccggtaatt atccactcgc gtgacgcggc ggaggatacc atgcggatct
     5641 taaaagagac cggaagattc cgggaacatc ccggtgtcat tcactgttac tcgtactcgc
     5701 cggagcaggc aaaagagtac gtgaagatgg gatactgcat tggtgtgggc ggtgtggtta
     5761 cctttaaaaa tgcaaagaaa ctcaggcaga cggtggagca gatctctctg gaacacattc
     5821 tgttggagac ggactgcccg tatatggcgc ccgagccgca ccggggaacc cgcaacgatt
     5881 cctccaacct ctcttatgtg gctgccagga ttgcagagat caaaggcgtc tctgtgcagg
     5941 aggtggagca gacgacctgg gaaaatgcct gccgtgtatt cggcgtccgg taggtgtctg
     6001 cccgaatcag cagaagcggg aatattccgg ggtgtctgcc caatagcgct tcgggattaa
     6061 ctgcatctgc aagcccggat ttttgcggga ttctatggca atgtgatcaa aaaaggtcag
     6121 ccagagctgc cggaaggctt cctcgtcgga ggagtagcgg ctgactttct ccagatccag
     6181 atctgcggca tctgtgagaa tgtagctgga aagcgctttg tgcaccgcga cagactgatg
     6241 tgtggcgtca tagatcataa agttctcgga gggcagacgg tcggtgaaat gctctgccag
     6301 atagggaagg acgttgtcct tcggatggat cgtggcaaag agaacgccgt tctcaagctc
     6361 ggagaagcgc agaaattcga gatgatggca ggcttcgcgg ttggtcgccc ggcagagctc
     6421 aaaaatccgg tagacacacg gttcgcctaa ggaaagaagc actttctgac cgtctccgct
     6481 tgcgagtgcg aggaggatgg tctcatagat ggcgtctgcc ttgtccatct tcctgccgga
     6541 ggatggctcg ccggacattg ctgcctgata gatcgactca tagaactgca tgccgaaacg
     6601 ggacgtgatg gtgcggatca ccttgccggt cttatccgcg gaaggagtta cgtgaatata
     6661 ttcggaaaaa agctcgtaat tctccggttc tccggtggca agccggatat tgcggtgacc
     6721 gagccggctt gcgcaggcgt catagacacc ggtaaagata ccttcaagac tgtcctcaca
     6781 gataaataca tacatgtaaa atacctcttt tctatgtgta atcagggcgg tgggctcaca
     6841 gggcaagccc ggtgtcaaac agcgacaact gctgataacc gctgttgcgg agctccgttg
     6901 gaacctgcgt gtggtctgcc atgagatgag agcagatata gtcctcgtcg agcttcgtgc
     6961 ggtacatcat ccggccggaa caggtgatga aatacagggc gcgctttagc acaacgccga
     7021 tctttttcag atcgtcatag gtgagcgcgg tatcccgtct ggcggcgacg atccgctggg
     7081 cggacttgta tccgattccg ggtacgcgca gaagagtctg ataactggcg cggttgatct
     7141 cgaccggaaa tgtgtcgaga tgacgcaatg cccagtcaca cttgggatcc agataaatat
     7201 taaagttcgg gcggtcttcc gacagaagct catccacatg aaagcggtaa tagcgcagca
     7261 gccagtctgc ctggtacagg cggtgctcgc gcagcagcgg aggtccgccg gggagcaccg
     7321 gcagtgacga atcgccgttg acattgatga atgcagagta gaaaacgcgc ttcagatgaa
     7381 acttctcata gagtgcctcg gacacacgca taatctgata gtcgttctcc ggggtagcac
     7441 cgataatcat ctgtgtgctc tgcccggcgg gcacgaactt cggcgctttg cggtagacca
     7501 tcagttcatt ctggttctcc gtgatgccat tctggatctg gcgcatgggg gagagaatcg
     7561 tgtgtcttga tttgcaggga gcaagctgct tcagtccgtt tgccgtggga agctcaagat
     7621 tgatgctcat gcggtccgca agaaaccctg ctttctcaat cagtgccggg tcagcgccgg
     7681 gaatcgcttt tacgtggatg tagccgttga agtgatggac ggtacggagc cggtagagcg
     7741 tctggtaaat cagatccatg gtgtaattcg gactgatgag gatgccggaa cttaagaaca
     7801 gaccctcgat ataattgcgc cggtagaatt ccatggtgag cgtgcagatc tcatccgggg
     7861 taaaggaggc gcgggggacg tcggagttgc agttgttctg gcagtaggca cagttaaaaa
     7921 tacactcgtt ggtgaacagg atcttgagca gggagatgca tcttccgtct gcagcaaatg
     7981 catggcacac gccgggagcc agacaattcc ccatgtgcct gccgtctccc ctgcggtcgg
     8041 cgccgcttga ggtacatgcc acgtcgtact tcgcggcatc ggtaaggatg gcaagcttct
     8101 ccatgaggga gagattctgc tgaatgttca tgtgaaaaca cctcctggca aaacaaatgt
     8161 tcggttaaag aatagcacat atgttcgaca aaagcaagat gaaatcgaat atttgttcga
     8221 gaaagattat ggttcacttg gacagtgggt atatggtata atgtacgtaa gcaatgagca
     8281 gtgccagacg tgacagtcac gagatatgcc tgcttgcagg ctatatctcg cgactgttgc
     8341 ggatgatagg gcgaatgccc gttagtccgg gaaatctatg atttcctgga cgagcactgc
     8401 gaagacgtga catttgccgg agcgggcatc ggagacttta gacggatgat agggcgaatg
     8461 cccgtc
//
LOCUS       DBMDQJ010000009         8387 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3693_length_8387_cov_1.666787, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000009 DBMDQJ010000000
VERSION     DBMDQJ010000009.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 8387)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 8387)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8387
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3693_length_8387_cov_1.666787"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="ACZO9R_00380"
     CDS             <1..375
                     /locus_tag="ACZO9R_00380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0911559.1"
                     /translation="IDIDNFKNVNDTYGHAFGDVVLKEVADICARHKGAAGFCARYGG
                     EEFAIIFKGVGVGQAEEMAEQIRQEFEQKMFTTPQGERSFSVSIGVAEYDRAYAGASA
                     FFEVADAALYRAKREGKNQVRR"
     gene            372..2102
                     /locus_tag="ACZO9R_00385"
     CDS             372..2102
                     /locus_tag="ACZO9R_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR0911560.1"
                     /translation="MKVLLTAVNAKYIHSNLAVYDLRAYAETYGTGGAQVEIAEYTIN
                     HTIDYILEGIYKAKPDVLCFSCYIWNLDYVEQLMDEYHKICPEVPIWVGGPEVSYETE
                     AFLKEHPQVTGVMTGEGEETFLELCESYAKAGIGEMAEKMNLSGIKGIMYRAGESLVK
                     TGAREPLDLNRIPFCYGGVEDFRNRIIYYESSRGCPFRCSYCLSSVEKTLRFRDVERV
                     KKELAFFLEQEVAQVKFVDRTFNCRHEHAMEIWKFLIEHDNGVTNFHFEISADLLTDE
                     EIALIGQMRPGLIQLEIGVQSTNDATITEIHRTMQLDRLKAVVRSIQEKENIHEHLDL
                     IAGLPYEGYETFARSFDEIYALKPNQLQLGFLKVLKGSYMYEHATEYGLIYRSRPPYE
                     VLKTNWLGFGEILRIRQVEEMLEVYYNSGQYATAIRLLETQYASAFAMFAELGTFYEE
                     HGYFGMGHSRMRRAEILLEFAKERRPGVLPVLGEGLVYDLYERENLGSRPAWAADANE
                     WKQMTRQYGKNGKQSHIERFFYRFSGRRQDTVLLPERLEDPVYVRFDYTGRDPLDHQA
                     RHEYLEPSGE"
     gene            2124..2840
                     /locus_tag="ACZO9R_00390"
     CDS             2124..2840
                     /locus_tag="ACZO9R_00390"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR0911561.1"
                     /translation="MLEDYVVIDLEMTGLNAKTDAILEVGAVRVRDGRQTETYGAILK
                     CGRELSERVVELTGITSEMVAGGREPEEAMREFFTFLGDDVLVGQNVIFDYSFLKQWS
                     VNHGQTFERNAVDTLKLARRFLPAEQKKDLESLCTYFGIGRERAHRALDDAMATGIVL
                     ERLKQEYGTVQPEAFLPYALCYRTKKQTPATGRQMDGLKKYAAHYGIPETEIPEQMTR
                     SEASRLLDRWIAVHGRMPRD"
     gene            complement(2837..3439)
                     /locus_tag="ACZO9R_00395"
     CDS             complement(2837..3439)
                     /locus_tag="ACZO9R_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911562.1"
                     /translation="MIFLGLFIFFVCMFAYLRHKSTAAEEAAAESFWNRENEANHTRR
                     KDISGLPYITIPLADFPMGIYDNPELKKYEETLQSLAGQKILNLGGKTNTDLKLEYGV
                     ANLETLCACDENYTTLCRTIYEYAECLKKLGHDAEAVRVLEYGISCGSDHSGNYRMLA
                     DYYLNARDSDALDRLLIAARALESPRQNAIVAMLEEKVNA"
     gene            3807..5345
                     /locus_tag="ACZO9R_00400"
     CDS             3807..5345
                     /locus_tag="ACZO9R_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR0911563.1"
                     /translation="MKKGTGFLTLFGGAVALYALLAAPVSMRAQETETIEDGVYIGNI
                     YVGGMTEEEAVSAVEAYVESADSAEMTLKTGDKSVSVTAADLGISFSNLNVVDEAIDV
                     GRSGNLIKRYKDKKDLQQGDKVIALSLDVDSDAVASILSEKAAQLNQEAVDNGLVREN
                     GAFKIIKGEQGIEVNVEDSIAAIENYISSEWDGGNAEIELVAEVVEPRGSEEDLEQIT
                     DMMGSYTTNYKDSGQNRCDNISNATSKINGTLLYPGEEFSVYEAIGPLDAANGYELAG
                     AYENGQTVESYGGGVCQVSTTLYNAVILAELEITQRSNHSMIVSYVKPSMDAAIAGDY
                     KDLRFVNNQDIPIYIEGYTEGKNVTFKIYGHDTRPSNRVVTYESEVVSEQDPGTQYVA
                     TGDPAGYMKTAQGKHIGYVARLWKVVTVDGVEESREIFNKSTYKASPKIVNVGTASAD
                     PNVSAVIGAALATGDEATINAAVAPYAASAAAILNPAPVEQPSQEEQAITGTVDESQI
                     TGGE"
     gene            5417..6400
                     /locus_tag="ACZO9R_00405"
     CDS             5417..6400
                     /locus_tag="ACZO9R_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase family protein"
                     /protein_id="HIR0911564.1"
                     /translation="MKIGKLPESVLKRSVFKQIHTRRPEVVLGAGVGEDCAAIKLAED
                     ETLVMSTDPITGTAKDIGTLAIQITVNDLASAGAEPVGVLLTVLLPESVEEPELRAMM
                     AQVEEACAKAKIQVMGGHTEVTAVVNQPVISVCGVGKVKDGCLISTGGARPGMDILVT
                     KWIGIEGTSIIAKEKETELKTKFSVPFVEKAKALDAYISVQSEAAVAVKSGVGAMHDV
                     TEGGIFGALWEMAEASGVGLEIDLKKIPVRQETIEVCEFFGINPYQLISSGCMLMAAE
                     DGNLLVRELEKAGIPATIIGKATAGNDRVLLNEEERRFLEPPKADELYKAI"
     gene            6414..6890
                     /locus_tag="ACZO9R_00410"
     CDS             6414..6890
                     /locus_tag="ACZO9R_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR0911565.1"
                     /translation="MREKILTFIEKNSRIDLKELAIVLGVDETTVMNELEKMEEEHII
                     CGYHTLIDWDKAGIEKVTALIEVRVTPQRGMGFDKVAERIYNYPEVNSVYLISGGFDF
                     MVTIEGKTLREVSQFVSDKLSPLDSVLSTKTNFILKKYKDHGTVMAEQPKDERIEM"
     gene            6892..8061
                     /locus_tag="ACZO9R_00415"
     CDS             6892..8061
                     /locus_tag="ACZO9R_00415"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0911566.1"
                     /translation="MRDPLNKTITTIQPSGIRKFFDVVHEMKDAISLGVGEPDFDTPW
                     HIRDEGIYSLEKGKTHYTSNAGLKELKVEIDHYLDRHYGVSYDPDHEIMVTIGGSEAI
                     DAAMRAMLDPGDEVLIPQPSYVSYVPCAVLAGGVPVIIELKAENEFRLTPEALEAAIT
                     PKTKLLVMPFPNNPTGAIMEKKDLEKIADIVREHDLYVISDEIYSELTYLERHVTIAS
                     LQGMRDRTIVINGFSKSHAMTGWRLGYACGPRIIIEQMLKIHQFAIMCAPSTSQYAGV
                     EALRNGDEDVAAMREEYNGRRRYLLHRFKEMGLSCFEPFGAFYVFPCIKEFGMTSEEF
                     ATELLNQQKLAVVPGTAFGDCGEGFVRISYAYSLDNLKKAMNRMEAFITDLRKRS"
     gene            8103..>8387
                     /locus_tag="ACZO9R_00420"
     CDS             8103..>8387
                     /locus_tag="ACZO9R_00420"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR0911567.1"
                     /translation="MAKLNVVLYEPEIPANTGNIGRTCVATGTRLHLIEPLGFHLDEK
                     AIKRAGMDYWSELDVTTYVNWEDFCEKNPGAKIYMATTKGRHVYTEVSYEP"
BASE COUNT         2171 a         1774 c         2616 g         1826 t
ORIGIN      
        1 atcgatattg ataattttaa gaatgtcaat gatacctacg gacatgcgtt tggagatgtc
       61 gttttaaaag aagtggcaga catctgcgcc agacacaaag gcgcggcggg attctgtgcc
      121 cgctatggcg gcgaggagtt tgccatcata tttaagggag tgggagtcgg acaggcggaa
      181 gagatggcgg agcagatccg gcaggagttt gaacagaaaa tgtttacaac gccgcagggg
      241 gagcggtcgt tttccgtgag catcggtgtg gcggagtacg acagggctta tgcgggtgcc
      301 agcgcatttt ttgaagtggc ggacgctgct ctttaccgcg ccaaaagaga gggcaagaat
      361 caggtgagac gatgaaagta ttgttgacgg cggtgaatgc aaaatacatt cactcaaatc
      421 tggcagtgta tgatctgcgc gcctatgcgg agacttacgg taccggcggg gcgcaggtgg
      481 agattgcaga gtacacgatt aaccatacga tagactacat attggaagga atttataagg
      541 caaagccgga tgtgctttgc ttttcctgtt acatctggaa tctggactat gtggaacagc
      601 tgatggacga gtaccacaag atctgtcccg aagtgccgat ctgggtcgga ggtccggaag
      661 tgtcctatga gacagaggcg tttctaaagg agcatccgca ggtgaccggg gtgatgaccg
      721 gcgagggaga agagactttt ctggaactgt gcgagagtta cgcaaaagcg ggcatcggtg
      781 agatggcaga gaagatgaat ctttcgggaa tcaagggaat catgtaccgt gccggagaga
      841 gccttgtaaa aaccggagcg cgcgagccgc tggatttaaa ccggattccg ttctgctatg
      901 gaggcgtcga agatttccgg aaccgcatca tctattatga atcgagcaga gggtgtccgt
      961 tccggtgcag ttactgtctt tcctcggtgg aaaagacact gcggttccgg gatgtggagc
     1021 gggtgaaaaa ggaactggca ttttttctgg agcaggaagt cgcgcaggtg aagtttgtgg
     1081 atcgcacctt taactgcagg catgaacatg ccatggagat ctggaagttt ttaatcgaac
     1141 atgataatgg cgtgaccaac tttcattttg agatatctgc cgatcttctg acggatgagg
     1201 agattgcgct gatcggtcag atgcggccgg gactgatcca gctggagatc ggcgtgcagt
     1261 ccacgaacga cgccacaatc accgagattc accgaacgat gcagctggac cgcctgaaag
     1321 cggtagtaag gagcatccag gaaaaagaga atattcacga gcatctggat ctgatcgcag
     1381 gtctgcccta tgagggctat gagacatttg cgcgttcgtt tgatgagatt tacgcgttaa
     1441 agcccaatca gcttcagctt ggatttttaa aagtgctaaa aggttcttac atgtatgagc
     1501 atgccacaga gtatggtctg atctaccgca gcagaccgcc gtatgaggtg cttaagacaa
     1561 actggctggg gttcggggag attcttcgca tccggcaggt ggaagagatg ctcgaggtgt
     1621 attacaacag cggacagtac gccacagcaa tccggcttct ggagacgcag tatgcgagtg
     1681 cgtttgccat gtttgcggaa ctgggaactt tttatgagga gcacggttat tttggaatgg
     1741 gtcattccag gatgcgcagg gcggagatcc tgctggaatt tgcaaaagag cgtaggccgg
     1801 gggtgctccc agtgcttgga gagggtctgg tatatgacct gtatgaaaga gagaatctcg
     1861 gaagcagacc cgcgtgggca gcagatgcaa atgaatggaa acagatgacg cgtcagtatg
     1921 gtaaaaatgg aaagcagtcg catattgagc ggtttttcta ccggttttcc ggccgcaggc
     1981 aggatactgt attgctcccg gagaggctgg aagatccggt ctatgtgaga tttgactata
     2041 ccggaaggga cccgctcgat catcaggcgc ggcatgaata tctggagcct tccggggaat
     2101 aaccggaaag aggggagaat agattgttag aggattatgt ggtcattgat cttgagatga
     2161 cgggattgaa tgcgaagacg gacgccattc tggaagtggg agccgtgcgc gtcagagacg
     2221 gcagacagac ggaaacgtat ggcgctattt taaagtgcgg gcgggaattg tcagagcgcg
     2281 tggtagaact gacgggcatc acgtcggaga tggtggcagg cggcagggag ccggaagagg
     2341 cgatgcggga attttttaca ttcctggggg acgatgtgct ggtcggccag aatgtgattt
     2401 ttgattacag ctttctgaaa cagtggtcgg tcaaccacgg tcagacgttt gagcgaaatg
     2461 ctgtggatac cttaaagctg gcgaggcgct ttcttccggc ggagcagaaa aaggatctgg
     2521 aaagtttgtg tacctatttc gggatcgggc gtgagcgggc gcaccgtgcg ctcgatgatg
     2581 cgatggcgac cggaatcgtg ctggagagat taaaacagga gtacggtacg gtacagccgg
     2641 aggcgtttct gccgtatgcg ctctgttacc gcacaaaaaa acagacgccc gcaacgggac
     2701 gtcagatgga tggcctcaag aaatatgccg cgcattacgg catcccggag acggagatcc
     2761 cggagcagat gacgcggagt gaggcgtcga gactgcttga ccgctggatc gccgtgcacg
     2821 ggcggatgcc ccgggattaa gcatttactt tttcctcaag catggcgacg atggcgttct
     2881 gtctggggga ttccagtgct ctcgcggcga tgagaagacg gtcaagtgcg tcggaatccc
     2941 tggcgtttag ataatagtct gcgagcatcc ggtagttccc actgtgatcg gacccgcagc
     3001 tgatgccgta ctcgagaacc cggactgcct ccgcgtcatg accgagcttt ttcaaacatt
     3061 cggcgtactc atagatggtg cggcagaggg tggtgtagtt ctcgtcacag gcgcagagcg
     3121 tttccagatt ggcaacgccg tactccagct tgagatcggt gttggtcttg ccgccgaggt
     3181 ttaagatttt ctgtccggca agcgattgga gagtctcctc atattttttt aattcgggat
     3241 tgtcatagat acccattgga aaatcggcaa gaggtatggt aatataggga agcccgctga
     3301 tgtccttgcg gcgtgtgtgg tttgcttcgt tctcgcggtt ccagaagctc tcggcagcgg
     3361 cttcttcggc agcggtgctt ttatggcgca gataagcaaa catacataca aaaaagataa
     3421 aaagtcctaa aaaaatcatg agaatcatcc cttcttgtgg tatagtgtaa aaggttcgcg
     3481 cggtatccag ggcgtgaacg gaaaggtggt agaacgaaat gtacgatttt ggtaaaaaaa
     3541 agaataaaaa actgcttgca ggaatcatca ttgtggttgt ggcagccatg cttttgacaa
     3601 cggtgcttgc agcgttatta taagtgtatt ccgccgtttt gctttcatgt aaaatatact
     3661 aaaaatccca gagtgagtca aacatattcg tgtttatgca ccttgaaaag aaaggcgcgt
     3721 atgggctata atgagcggaa gcagagtata tgcttggcaa aacaggcgtt agcagagcag
     3781 agaacagata tttcagaggg aagaatatga aaaagggaac aggttttttg acattgtttg
     3841 ggggtgcagt ggcactttac gcattgctgg cggcaccggt ttccatgagg gcacaggaga
     3901 cggagacgat tgaggacggt gtctatatcg gcaatattta tgtgggcggc atgacggagg
     3961 aagaagcggt cagcgcggta gaagcgtacg tggagagcgc agattcggcg gagatgacat
     4021 tgaagaccgg cgataaaagt gtatccgtga cggcagctga tcttggaata tccttttcca
     4081 acttaaacgt ggtggacgag gcgatagatg tcggcagaag cggcaacctc atcaagcggt
     4141 acaaggacaa gaaggatctg cagcaggggg acaaagtgat tgcactctca ctggatgtgg
     4201 attccgatgc ggtggcatcc atcctttccg agaaagcggc gcagctgaac caggaggctg
     4261 tggataatgg tctggttcgt gagaacggtg cgtttaagat cattaagggc gagcagggca
     4321 tcgaggtcaa cgtggaggat tccattgcgg cgatcgagaa ctatatcagc agtgagtggg
     4381 acggtggcaa cgccgagatc gagctcgtgg cggaagtggt ggagccgcgc ggcagcgagg
     4441 aagatctgga gcagatcacg gatatgatgg gaagctatac gacgaattac aaggattccg
     4501 gtcagaaccg ctgcgacaac atctccaatg caaccagcaa gatcaatggt acactgcttt
     4561 atccgggcga ggaattttcc gtctatgaag cgatcggacc gctggatgcg gcaaacggat
     4621 acgaactggc aggtgcctac gagaatggtc agacggttga gtcctacggc ggcggtgtgt
     4681 gccaggtatc tacgactttg tacaacgcag tcattctggc ggagcttgag atcacgcagc
     4741 gctcgaatca ttccatgatc gtaagctatg tgaagccatc catggatgcg gcgattgcag
     4801 gcgattacaa ggatctgcgt tttgtcaata atcaggacat tccgatttat attgaggggt
     4861 acaccgaggg aaagaatgtc acctttaaga tctacggaca cgacacgaga ccatcgaacc
     4921 gcgttgtgac ttatgagagt gaagtggtat ccgagcagga tccgggcacg cagtatgtgg
     4981 cgaccggaga tccggcgggt tatatgaaga ctgcgcaggg caagcatatc ggctatgtgg
     5041 caagactgtg gaaagttgtc acggtagacg gtgtggagga gagccgggag atttttaata
     5101 agagtaccta caaggcgtct ccgaagattg taaacgtggg aacggcatca gcagatccga
     5161 atgtgtctgc ggtgattggc gcggcgcttg cgaccgggga tgaggcaacc atcaatgcgg
     5221 cggttgcacc ttatgcggcg agtgcggcag caatcttaaa tccggcaccg gtagaacagc
     5281 cgtcgcagga agaacaggcg attaccggaa cggtggatga gagccagatc acaggcggag
     5341 aataaaagta taagagattg tttaaggctg cctcagaaga gacagcctta agctgtaaat
     5401 aaacgggaga acacagatga agatcggaaa actgccggag agcgtactga agcgctccgt
     5461 attcaaacaa atacatacca gacgccccga ggttgtctta ggagccggcg tcggagagga
     5521 ctgcgccgcg atcaagctgg cagaggatga gacgcttgtg atgtccacag atccgattac
     5581 cggaacggcg aaggacatcg ggacacttgc gattcagatt acagtgaatg atctggcaag
     5641 cgcgggggcg gagccggtgg gcgtgctgct cacggtgctg ctgccggaga gcgtggagga
     5701 gcccgaactg cgtgccatga tggcacaggt ggaggaagcc tgcgccaagg caaagatcca
     5761 ggtgatgggg ggacacacgg aagtgacggc ggtggtcaat cagccggtga tctccgtctg
     5821 cggtgtcggc aaagtgaagg atggctgtct gatctcgacc ggaggggcga ggccgggtat
     5881 ggatattctt gtcacaaagt ggatcggaat tgagggaact tccattatcg caaaggaaaa
     5941 agaaacagaa ctaaagacaa agttctcggt gccttttgtc gagaaggcaa aagcactgga
     6001 tgcttatata tcggtacaat cagaggccgc agtcgccgtc aagtctggcg ttggtgcaat
     6061 gcatgacgtc acagagggcg gaatattcgg tgcgctctgg gaaatggcgg aagcatctgg
     6121 cgtcggactt gaaatcgatt tgaaaaagat accggttcgt caggagacca ttgaggtctg
     6181 tgaatttttc ggaatcaacc cctaccagct gatttccagc ggctgtatgc tgatggcggc
     6241 agaggatggc aatctgctgg taagagaact ggagaaggca ggaatccctg caacgattat
     6301 cggaaaggca accgcgggga acgaccgcgt ccttttgaac gaggaggaac gcagattttt
     6361 ggagcctccg aaggcggacg aattatataa ggcgatataa aggagaagaa gcgatgcgtg
     6421 aaaagatttt gacatttata gagaagaaca gccggattga cttaaaggag cttgccattg
     6481 tgctcggtgt cgatgagaca acggtcatga atgagcttga gaaaatggaa gaggagcaca
     6541 ttatctgcgg ctaccacaca ctgatcgatt gggacaaagc gggcattgag aaggtgactg
     6601 cgctaatcga ggtacgtgtg acaccgcagc gcggcatggg ctttgacaag gtggcagagc
     6661 ggatctacaa ctatccggag gtcaattccg tttacctcat ctccggaggc tttgatttta
     6721 tggtgacgat cgagggaaag accctgcgcg aggtttcaca gttcgtgtcc gataagcttt
     6781 ccccgctgga ttccgtgctc agcacgaaga ccaacttcat cctgaagaag tataaggatc
     6841 atggaaccgt aatggcagag cagcccaaag atgaaaggat agagatgtaa gatgagagat
     6901 ccgttaaata agacaatcac aacgatccag ccgtctggta tccgaaagtt ctttgacgtc
     6961 gtccatgaga tgaaggacgc gatctctctg ggtgtcggcg agccggattt tgataccccg
     7021 tggcatatcc gcgacgaggg tatttattcc ctggaaaaag gaaagacaca ctatacatcc
     7081 aatgccggac tcaaggaatt aaaagtggag atcgaccatt atctggaccg gcattacggg
     7141 gtgtcctatg atccggatca tgagatcatg gtgacgatcg gaggcagcga ggcgatcgat
     7201 gcggcgatgc gcgccatgct tgatccggga gatgaggttt taatcccgca gccgagctac
     7261 gtgtcctacg tgccgtgcgc cgtgcttgca ggaggcgttc cggtgatcat tgagctgaag
     7321 gcggagaatg aattccgtct gacgccggaa gcgctggagg ctgccatcac accgaagacc
     7381 aagcttctgg tgatgccgtt cccgaataat ccgaccggcg ccattatgga gaaaaaagat
     7441 ctggaaaaga tcgcggacat cgtaagagag cacgaccttt atgtcattag cgacgagatc
     7501 tactcggagc tgacgtatct ggagcggcat gtgacgattg cctccctgca gggaatgcgt
     7561 gaccggacga tcgtgatcaa cggtttctcc aaatcccatg ccatgacagg ctggcgtctg
     7621 gggtatgcgt gcggaccgcg catcatcatc gagcagatgt taaagattca tcagttcgcg
     7681 attatgtgtg cgccgtccac cagccagtat gcgggcgtgg aggcactgcg caacggagat
     7741 gaggatgtgg ctgccatgcg cgaggagtat aacggcagac gccgctatct gctgcaccgg
     7801 tttaaagaga tgggattgtc ctgttttgag ccgtttggcg cattttacgt gttcccgtgc
     7861 atcaaggagt tcggaatgac gtccgaagaa tttgcgacgg agctgctcaa tcagcagaaa
     7921 ttagccgttg tcccgggcac tgcgttcgga gactgcggag agggatttgt gcgcatttcc
     7981 tatgcttact cgctggataa cctgaagaag gcgatgaacc gcatggaggc gtttatcaca
     8041 gatcttcgca agagatcata agaaaaaggg aggagggcgt ttcctcccga atggagactg
     8101 acatggcaaa gctgaatgtt gtattatatg agccggagat tccggcaaac accggaaata
     8161 tcggcagaac gtgcgtggcg acagggacaa gactgcacct gatcgaaccg ctgggattcc
     8221 atctggatga gaaagcaatc aagcgtgcgg gcatggatta ctggagcgaa ctggatgtga
     8281 ccacctatgt caactgggaa gatttctgcg agaagaatcc gggtgctaaa atttatatgg
     8341 caacgacgaa gggcagacat gtatacaccg aggtatccta tgagccg
//
LOCUS       DBMDQJ010000010         7985 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_3914_length_7985_cov_1.500888, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000010 DBMDQJ010000000
VERSION     DBMDQJ010000010.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7985
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3914_length_7985_cov_1.500888"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(10..2094)
                     /gene="cobK"
                     /locus_tag="ACZO9R_00425"
     CDS             complement(10..2094)
                     /gene="cobK"
                     /locus_tag="ACZO9R_00425"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HIR0911568.1"
                     /translation="MAEILIFSGTTEGRELAEALCARQIDCMASVATEYGREVMRQQE
                     HLVIREGRMGESEMEELMRTGAFLAVVDATHPYAVEVTEHIKESAKKTNLPYLRLSRS
                     TAAEREIAEHEWMIHTVADTQECVELLSKLPGNILLTTGSKELHAYAAREEIRERLFV
                     RVLPGVESIEICHREQIPGKQIIAMQGPFGTELNEALIRQYDIGVLVTKESGQAGGFP
                     EKIRAAEHTKIPVVMIQNPEQAGGMSMEKVLSEIETLCGKKSGTKHQPLQISLVGIGV
                     GNTKLFTGEAREAIAQAEVVCGAKRLLAAADPLIAPDAEKREAYLPADLLPYIYSCRE
                     KGTVRIAILFSGDTGFYSGAEAVHKALQEEVRLGKLQAGITICPGISSLSYLAARAGK
                     SWQDAKIVSTHGREADVTACVRREKKVFLIVSGRSDLIRIGEELEAAGLSGVELTIGY
                     QLSYREERIWHGTPEVCRTLKDDGLYACLIENPGAQGETLAPGVPDRWFVRGRVPMTK
                     EEIRWLSLCRLGLKSQSVFYDIGSGTGSIAVEAALRSKQLRVYAVEHGEEACGLIAEN
                     ASRAGVKNLVVVKGEAPECLGELPAPTHAFIGGSGGRLREILLALREKNPNVRVVLNA
                     VSLETVAEVVALRKELLICEEEITQIAVSRAKKAGSHHLMQAENPIYVISFSLSENGE
                     GEEQKPDRREER"
     gene            complement(2117..2887)
                     /gene="cobJ"
                     /locus_tag="ACZO9R_00430"
     CDS             complement(2117..2887)
                     /gene="cobJ"
                     /locus_tag="ACZO9R_00430"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HIR0911569.1"
                     /translation="MKHEIYIVGMGPGREEDMTQEALTVLDSCDVIVGYTVYLNLLGE
                     RFLGKTFLSTPMRQEVERCRMCYEEAKKGKRVALICSGDAGIYGMASLMYEMSAEYPS
                     CELTVVPGITAASSGAAVLGAPLNHDFCVISLSDLLTPWEQIKARLRAAVLGDFAIAL
                     YNPSSRKRADYLQRACDILLDAGAKGTRACGYVENIGRDGETAHTCTLQELREIQVNM
                     FTTVFIGNSQSVIMGDKLVTKRGYQIGTGARARADERG"
     gene            complement(2887..3942)
                     /locus_tag="ACZO9R_00435"
     CDS             complement(2887..3942)
                     /locus_tag="ACZO9R_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014006.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HIR0911570.1"
                     /translation="MKKTDIAVISFTYRGAELAERIQEAMDAVWFCKLYTKCSDARAE
                     GIGISVDQPLAEWTGKQFAEGNALLFIGACGIAVRSIAPHVKDKLSDVPVLVADEAGQ
                     FVIPILAGHYGGANRLAGELSRALGATAVLTTATDVNGLFAVDVFAASNRLAVAGHDG
                     IARVSAGLLRAGYLTMSVAGECEGEIPPEVRLVPYPPKEPVDVLVAPQCEAGERCSLW
                     LIPSCLLLGVGCRRGKSEEELEAFVRETLEKEKLSSMAVAGIASVDVKADEVGILALA
                     EQLAVPFLTYPAGRLQCVDGTFTSSGFVAQQVGVDNVCERAAVCAAGEGGRLLVQKTA
                     CEGKTLAIAEKKWSVKF"
     gene            complement(3939..4715)
                     /gene="cobM"
                     /locus_tag="ACZO9R_00440"
     CDS             complement(3939..4715)
                     /gene="cobM"
                     /locus_tag="ACZO9R_00440"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HIR0911571.1"
                     /translation="MVYFVGAGPGAKDLITVRGMRLLEQADVIIYAGSLVNPELLSYA
                     GSGAKIYDSAKMTLEEVLAVIREAEEKGLTTVRLHTGEPSLYGAVREQMDVLAAEGIS
                     YESCPGVSACFGAAADLNLEYTLPGISQSLIITRMAGRTAVPERESIESFAAHQASMA
                     VYLSAGMVKELSKRLMDGGYAPDTPAAIVYKATWPDEEAYVCTVETLAAVAVEHQITK
                     TALILVGDVLAAPGYERSKLYDPGFTTGYRKAKKEQGETK"
     gene            complement(4738..>5408)
                     /gene="cbiD"
                     /locus_tag="ACZO9R_00445"
     CDS             complement(4738..>5408)
                     /gene="cbiD"
                     /locus_tag="ACZO9R_00445"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HIR0911572.1"
                     /translation="LAKKTFNPRLGIVGGISILGTTGIVEPMSSQALLDTIYVELKQK
                     REEGKRFAAISPGNYGLEYMKRAYGFDLDRSVKCSNFIGKTMDMAVELGFEAVLLTGH
                     IGKLVKLSGGIMDTHSREADARMELLMAAAYRAGCAPDTLGKILDCLVTEEALAYIAK
                     DGCLSATMELLMKKILFYLKKRVKEELCVECILYANGYGELARSDGAVELLERIRGEE
                     EHLQ"
     assembly_gap    5409..5418
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5419..5877)
                     /locus_tag="ACZO9R_00450"
     CDS             complement(<5419..5877)
                     /locus_tag="ACZO9R_00450"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HIR0911573.1"
                     /translation="MRYGFTTGSCAAAAAKAAAYMLLTGREKHEITITTPKGILYYAR
                     MEEISRTEHSVRCAVRKDGGDDPDITTGTLIFAEVSWQEQKQEKDCGKPQILITGGAG
                     VGTVTRPGLDQPVGSAAINHVPREMITREVEEVCALTDYAGSLLVEISVQG"
     gene            complement(5882..7603)
                     /locus_tag="ACZO9R_00455"
     CDS             complement(5882..7603)
                     /locus_tag="ACZO9R_00455"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HIR0911574.1"
                     /translation="MVQQNIHGGDIYRNQVSLDFSVNINPFGMPQRVREALEAAVAWC
                     ERYPDIRSEALRDAIAGAKGIAPADIVCGNGASELFPAILRAVHAKKVLLPVPSFYGY
                     EYAAKSVGAEICCHELLEADGYAVTERFLTAIGEGVNAVFLAVPNNPVGTVVEPELLE
                     KIAERCQKYGVTLILDECFVMFTGAEERYSFLKKYRNYPGVVIVRAFTKLYAMPGVRL
                     GYLVCSDAALTEAIRADLPEWNLSVFAQQAGCVACGQRAYVEHSVAFVCRERAWLTEQ
                     LSEIGITVFASNANFLMIKDGRPLAKELLKKGILIRDCSNYRGLSGGYYRIAVRRREE
                     NERLLAALAQVTGSSVVENGKDDKKPAAVPDGIEYVMPQEIEKRSFSIIEEELQLRGI
                     TLPVEEAMVTKRVIHTSADFSYAQTMTYSAGAVETAKWLITEGADIVTDTNMALSGIN
                     KRVLARYGGSVHCFMADEDVAREAKERGVTRATVSMERAAKIEKPVIFAIGNAPTALI
                     QLYGMIEEGYRPAFIIGVPVGFVNVEAAKELILQTKIPHIVNRGRRGGSNVAAAICNA
                     ILYELGR"
     gene            complement(7634..>7985)
                     /locus_tag="ACZO9R_00460"
     CDS             complement(7634..>7985)
                     /locus_tag="ACZO9R_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobD/CbiB"
                     /protein_id="HIR0911575.1"
                     /translation="FIPARISAMLMILSCCFLGNEFDQKNAMKIFKRDRYQHASPNSA
                     QTEAACAGALGIRLAGDASYFGRIVKKPYIGDPLRAVETEDIRRANRLLYGMAFFGWG
                     ICMAVWLCVTAIVW"
BASE COUNT         1612 a         2708 c         1779 g         1876 t
ORIGIN      
        1 atgttcatct tacctctcct cccgtctgtc cggtttttgt tcttccccct cgccattctc
       61 cgacagggaa aacgagatca catagatcgg attctccgcc tgcatcagat gatgggaacc
      121 tgcttttttt gcgcggctga ccgcaatctg tgtgatctct tcctcacaga tcaaaagctc
      181 cttccgcagt gccacaacct cggcgaccgt ctcaagcgag acggcattta agacaacgcg
      241 cacgttcgga tttttctccc ggagtgccag aagaatctcc cgcagcctgc cgccgcttcc
      301 gccgatgaac gcgtgcgtcg gcgccgggag ttctccaaga cattccggtg cctccccctt
      361 tacgacaaca aggttcttca cgcctgcacg cgatgcattt tccgcaatca gtccacaggc
      421 ttcctctcca tgttccacgg catacacccg cagctgtttt gaccgcagcg ccgcctccac
      481 cgcaatcgag ccggttccgc ttccgatatc ataaaatacg ctctgactct ttaacccgag
      541 cctgcacaga gacagccagc ggatttcctc cttcgtcatg gggacacgtc cgcgcacgaa
      601 ccagcggtca gggactcccg gagcgagcgt ctccccctgt gcacccggat tttcaatcag
      661 gcaggcatag agtccatcgt cctttaacgt ccggcacact tccggcgttc cgtgccagat
      721 ccgctcttcc ctgtaggaga gctggtatcc aatggtaagc tccaccccgg acagtcccgc
      781 cgcctcaagt tcttccccga tgcggattag atcgcttctg cctgaaacaa tcagaaatac
      841 ctttttctcc ctgcgcacac acgcggtaac atccgcctct ctcccgtgtg tgctgactat
      901 tttcgcgtcc tgccagcttt tacctgcgcg cgccgccaga taggagagcg aggagattcc
      961 aggacagatg gtgatcccgg cctgtaattt tccaagacgc acttcttcct gcaatgcctt
     1021 atgcaccgcc tctgcgccgc tgtaaaatcc ggtatcgccg gaaaataaga tggcaatccg
     1081 cacggttcct ttttccctgc aggaataaat atacggaaga agatctgccg gaagatacgc
     1141 ctcccgtttt tctgcatccg gcgcgatcag aggatcagcc gcggcaagca gccgcttcgc
     1201 tccgcagacc acctccgcct gcgcaatcgc ctcccttgcc tcgccggtga acaactttgt
     1261 gttccccact ccgattccca cgagagaaat ctgcagcggc tgatgcttcg tcccgctctt
     1321 tttcccgcag agtgtttcga tttcggacag gactttttcc atgctcatcc cgcccgcctg
     1381 ctccggattc tgaatcatga ccaccgggat tttcgtgtgc tctgccgcgc ggatcttctc
     1441 cggaaatccg cccgcctgtc cgctctcctt ggtcaccagc acgccgatgt catactgccg
     1501 gatcagcgcc tcgttgagct ccgttccaaa aggtccctgc atggcaatga tctgcttccc
     1561 cggaatctgt tccctgtggc agatttcgat gctctccacc cccggaagca cacgcacaaa
     1621 aagtctctca cggatctctt cccgggcggc atatgcgtgc agttccttgc tgcccgtcgt
     1681 aagaagaatg ttccccggca gcttagacag cagttctaca cattcctgcg tgtccgccac
     1741 cgtatgtatc atccactcgt gctctgcgat ctcacgctcc gctgccgtgc tccggctgag
     1801 ccttaaatag ggaagattgg tcttttttgc gctctctttg atgtgctccg tcacctccac
     1861 cgcgtaggga tgcgtggcgt ccaccactgc aagaaatgca ccggttcgca tgagttcttc
     1921 catctccgat tcgcccatcc gcccttcccg gatcacaaga tgctcctgct gacgcatcac
     1981 ttccctgccg tactcggtcg ccacactcgc catacagtca atctgtctgg cacagagtgc
     2041 ctccgctaat tctcttccct ccgttgtccc ggaaaaaatc aatatttctg ccatattctc
     2101 tccatttcca ggtctcttat ccgcgctcat ctgcacgcgc cctggcgcct gtcccgatct
     2161 gatagccgcg ctttgtgaca agcttatctc ccataatgac cgactgtgag ttgccgataa
     2221 ataccgtggt aaacatattc acctgaatct cacgaagttc ctgcagggtg caggtgtgtg
     2281 ccgtctcccc gtctcttccg atgttttcca catagccgca ggcacgcgtt cccttcgccc
     2341 cggcatccag cagaatatca caggcgcgct gcaaataatc tgcgcgcttc ctgctcgacg
     2401 gattgtagag cgctatcgcg aaatccccca gaacagctgc ccgaagccgc gctttaatct
     2461 gctcccacgg agtcagaaga tcgcttaagc tgatcacgca gaagtcatgg tttaacggcg
     2521 cgccgagcac tgccgcacca ctgcttgccg cggtaatccc cggtaccacc gtaagttcac
     2581 aggacggata ttctgcgctc atctcataca tgagtgacgc catcccgtag attcctgcgt
     2641 ccccgctgca gatcagcgct acgcgctttc ccttcttggc ctcctcatag cacatcctgc
     2701 atcgctccac ttcctggcgc atcggagtcg acaaaaacgt tttccccaaa aatcgttccc
     2761 cgagaagatt cagatacacc gtatatccca caattacgtc acaggaatcc agcacggtga
     2821 gcgcttcctg tgtcatatct tcctctctgc ccggtcccat tccgacaata tagatttcat
     2881 gtttcatcaa aacttcacac tccatttttt ctctgctatg gcaagcgtct tgccctcaca
     2941 tgccgtcttt tgcaccagaa gtctgccgcc ctcccccgcc gcgcagaccg ccgcgcgctc
     3001 acacacatta tccacgccga cctgttgcgc cacaaagccg gatgacgtga atgtaccgtc
     3061 cacacactgc aggcgcccgg ccggataagt gagaaaagga accgctaact gctctgccag
     3121 tgccagaatt cctacctcat ccgcttttac atcgaccgac gcgattcccg ccaccgccat
     3181 gctggaaagc ttctcttttt ccagggtctc ccgcacaaac gcttccagtt cctcttcgct
     3241 tttgcccctt ctgcacccga cgccaagcag caggcaggac ggaatcagcc ataagctgca
     3301 tcgttccccg gcttcgcact gcggcgcgac cagcacatcc accggctcct ttggcggata
     3361 cggtaccagt cttacctccg gcggaatctc tccttcacat tctccggcga cggacatggt
     3421 aagatacccg gcgcgcagaa gcccggcgga cacccgggcg attccgtcat gaccggcaac
     3481 ggcaagacgg tttgacgctg caaacacatc caccgcaaaa agcccgttca catccgtcgc
     3541 cgtggtgagc acggcagtcg caccgagggc tcttgaaagt tccccggcaa gccggttggc
     3601 tcctccgtaa tgacctgcaa gaatcgggat cacaaactgc cccgcctcat ccgccacgag
     3661 caccggaaca tcggaaagct tatcttttac atgcggtgcg atactcctga ccgcaatccc
     3721 gcatgccccg ataaacagga gcgcatttcc ctccgcaaac tgttttcctg tccattcggc
     3781 aagtggctga tccacggaaa tccctatccc ctctgcccgg gcgtcgctgc attttgtata
     3841 taatttacag aaccatacgg catccatcgc ttcctgaatg cgttctgcca gttctgcccc
     3901 cctgtaagta aagctgatga cagctatgtc cgtcttcttc actttgtctc tccctgttcc
     3961 ttttttgcct tccggtatcc cgtggtaaat ccgggatcat acagcttcga gcgctcatac
     4021 cccggcgctg ccagcacatc tcccaccaga atcagcgccg tcttcgtaat ctgatgctcc
     4081 accgccacag cagcaagcgt ctccaccgtg cacacgtacg cctcctcgtc cggccaggtc
     4141 gccttataga cgatcgctgc cggcgtatcc ggcgcatacc ctccgtccat cagtcttttc
     4201 gacagttctt ttaccattcc cgcacttaag tagactgcca tggacgcctg atgtgcggca
     4261 aaactctcga tgctctcccg ctccggcact gccgtgcgtc ctgccatccg cgtgataatc
     4321 aggctctgcg agatccccgg cagcgtatac tccagattca gatccgccgc cgcgccaaag
     4381 catgcgctga cgccgggaca gctctcatag gaaatcccct ccgctgcaag tacatccatc
     4441 tgttcacgca ccgccccgta gagactcggc tccccggtgt gaagccgcac ggtggtaagc
     4501 cctttttcct ccgcctcccg gatgaccgca agcacttcct ccaacgtcat ttttgcgctg
     4561 tcatagatct ttgccccgct tccggcataa gaaagaagct ccggattcac aagcgatccc
     4621 gcatagataa tgacatccgc ctgctccagc agtctcattc cgcggaccgt gattaaatcc
     4681 tttgctcccg gacccgcccc gacaaaatat accataccgt ttcctcctta aactgcgtta
     4741 ctgcaaatgt tcttcttccc cgcggattcg ttccagaagt tctaccgcac cgtcgcttct
     4801 cgccagctcg ccgtacccgt tcgcatagag aatgcattcc acacacagtt cctctttcac
     4861 ccgcttcttt aaataaaaaa gaatcttttt cattaaaagt tccatcgtcg cggaaagaca
     4921 tccgtccttg gcaatgtagg cgagcgcctc ctccgtcaca agacagtcta agatcttccc
     4981 cagcgtatcg ggcgcacacc cggcgcggta tgccgcagcc atcagaagct ccatgcgcgc
     5041 gtccgcctcc ctggaatgtg tgtccataat tccgccggag agctttacca gctttccgat
     5101 gtgtccggtc agaagcaccg cctcaaatcc gagctccacc gccatatcca tcgtcttccc
     5161 gataaaattg ctgcacttga cgcttcggtc cagatcgaac ccgtaagcgc gcttcatata
     5221 ttccagaccg taatttcccg gtgagattgc cgcaaaccgt tttccctctt cgcgcttctg
     5281 cttaagctcc acatagatcg tgtcaaggag cgcctgactg ctcatgggct ccacaatccc
     5341 ggtcgttcca aggattgaga taccgcccac aataccgagt ctcgggttga acgtcttttt
     5401 ggcaagttnn nnnnnnnncc cctgcaccga aatctccacc aaaaggctcc ccgcataatc
     5461 ggtaagcgca cacacttctt ccacctcgcg ggtaatcatc tcccgcggca catgattgat
     5521 cgccgcgctc ccgacgggct ggtctaaccc cggtcttgtg accgttccga cacccgcgcc
     5581 tcccgtaata agaatctgcg gttttccgca gtctttttcc tgtttctgct cctgccagga
     5641 tacttccgca aaaatcagtg ttcccgtcgt gatgtccgga tcatctccgc catcttttct
     5701 gacagcacag cgcacggaat gctccgtgcg gctgatctct tccatcctgg catagtagag
     5761 gatgcctttt ggcgtggtga tggtaatctc gtgcttttct ctgccggtaa gaagcatata
     5821 agccgccgcc tttgccgccg ctgcggcgca gcttcccgtc gtaaatccat agcgcatggg
     5881 ctcacctccc aagctcgtag agaatcgcat tgcaaatggc agccgcgaca ttgctgccgc
     5941 cccttcttcc acggtttaca atatgcggta tcttcgtctg taaaatcagt tccttcgccg
     6001 cctccacatt gacaaacccg accggaacgc cgatgatgaa cgccgggcgg tagccctcct
     6061 ctatcatccc gtatagctgg attaaagctg tcggcgcatt tccgatcgca aagatgaccg
     6121 gtttttcgat ctttgccgcc cgttccatgc tcacggtggc gcgggtaacg ccccgctcct
     6181 tcgcctctct tgccacatcc tcgtccgcca taaagcagtg taccgatccg ccgtagcgtg
     6241 cgagcacgcg cttgttgatg ccggagagcg ccatgttggt gtccgtcaca atgtccgcgc
     6301 cctctgtgat gagccacttt gccgtctcca ccgctcccgc ggagtaggtc atcgtctgcg
     6361 cataggaaaa atcggcgctc gtgtggatca cacgcttcgt gaccattgct tcctccacag
     6421 gcagcgtaat cccccgaagc tgcaattctt cctcgatgat ggaaaagctg cgcttctcta
     6481 tctcctgcgg catcacatac tcgattccgt cgggcactgc tgccggcttt ttatcatctt
     6541 tgccgttttc caccacgctg cttcccgtca cctgcgccag cgccgcaagc aggcgttcat
     6601 tctcctcgcg ccgtctgacc gcaatccggt aatatccccc ggaaagtccc cggtagttgc
     6661 tgcagtcgcg gatcagaatc cccttcttta agagctcttt tgccagcgga cgcccgtcct
     6721 ttatcatcag gaaattggcg tttgacgcaa acacagtgat gccaatttcc gacagctgct
     6781 ccgtaagcca ggcacgctcc ctgcagacaa atgccaccga gtgctccaca tacgcacgct
     6841 gcccgcaggc cacacatccc gcctgctgcg caaacaccga cagattccac tccggcagat
     6901 cggctcttat cgcttccgta agagcagcat cgctgcagac gagatagccg agccgcactc
     6961 ccggcatggc atagagcttc gtaaatgccc gcacgatcac aactcccgga taattccgat
     7021 acttttttaa gaaggaatag cgttcttccg ctccggtaaa catcacaaaa cattcgtcca
     7081 gaatcagggt cacaccgtac ttctgacagc gctctgcaat cttctccaac agttccggct
     7141 ccaccaccgt acccaccgga ttattcggga ctgccagaaa aacggcattc acaccctccc
     7201 cgatggcagt aagaaagcgc tccgtcaccg cgtagccgtc cgcctcaagc agttcgtggc
     7261 agcagatctc tgctcccaca gactttgccg catactcgta tccgtaaaat gacggtacag
     7321 gaagcagcac ctttttcgca tgaaccgctc ttaaaatggc aggaaacagt tctgacgcac
     7381 cgtttccgca cacaatatcc gcaggcgcaa tcccttttgc cccggcgatg gcatcccgca
     7441 atgcctccga ccggatatcc ggataccgct cacaccatgc cactgccgcc tcaagcgcct
     7501 cacgcacccg ctgcggcatc ccgaacggat tgatgttgac cgaaaaatca agcgatacct
     7561 gattgcggta tatgtctcct ccatgtatgt tctgctgcac catcccggtt cttcctcctg
     7621 ttctgtctct atgctaccac acaatggctg tcacgcacaa ccagacagcc atacaaattc
     7681 cccatccgaa aaatgccatc ccgtacagca ggcggttggc acgtctgata tcctctgtct
     7741 ccactgctct tagcggatca ccgatgtacg gttttttgac gattcttcca aaatagctgg
     7801 catctcctgc cagtcttatg ccgagcgctc ccgcacatgc cgcctcggtc tgtgcggagt
     7861 tcgggctggc atgctgatac cggtcccgct taaaaatctt catcgcattc ttctggtcaa
     7921 attcgtttcc aaggaaacag caggataaga tcatgagcat ggcgctgatg cgcgccggaa
     7981 tgaaa
//
LOCUS       DBMDQJ010000011         7811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4006_length_7811_cov_1.732166, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000011 DBMDQJ010000000
VERSION     DBMDQJ010000011.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7811
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4006_length_7811_cov_1.732166"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="ACZO9R_00465"
     CDS             complement(<1..206)
                     /locus_tag="ACZO9R_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911576.1"
                     /translation="MQILAWTAELVLMPVLQALSARVDFSHISDDSMKGSFLWSSPFS
                     NTFYTPFFFFPFFSEAAAASLLAI"
     gene            complement(136..306)
                     /locus_tag="ACZO9R_00470"
     CDS             complement(136..306)
                     /locus_tag="ACZO9R_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR0911577.1"
                     /translation="MAYVISDSCVSCGTCESECPAGAISQGDDHYVIDADSCMDCGTC
                     ADACPTGAISEG"
     gene            complement(468..1505)
                     /locus_tag="ACZO9R_00475"
     CDS             complement(468..1505)
                     /locus_tag="ACZO9R_00475"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR0911578.1"
                     /translation="MKITKSDFGTLNTGENIYIYHMENANGAYVEVINFGCRLVKIVV
                     PDRDGKMTDVCLGFDSMEDYARDNASLGAVVGRVANRIKDGHFCLDGKEYQLAINNGS
                     NHLHGGLIGYASKPWDAKIKDDKLILTMNSYDGEEGYPGNLTLSVTYGWSEDNELSLL
                     YEASTDADTLLNVTSHGYFNLNGEGSSEVLSHELFIDADQITELDDSQAPTGRFLPVE
                     GTPFDFRSMHTIGKLMDSDYEQYHKFGTYDHNFVINGTGFREAAVLQSKESGIRMTCF
                     TDQPGMQLYVANQPIEVTGKNGTQYACHTSVCLETQHFPDAINHDNFPSIVLTPDEPF
                     RSKTLYHFSTF"
     gene            1758..2810
                     /locus_tag="ACZO9R_00480"
     CDS             1758..2810
                     /locus_tag="ACZO9R_00480"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR0911579.1"
                     /translation="MQTKVVKIDSANIDDAAMKEACDLIRAGELVAFPTETVYGLGAD
                     ALHPEASKKIYAAKGRPSDNPLIVHISKFEDLVSIAREVPPQAKKLADAFWPGPLTMI
                     VWKNDRVPYETTGGMDTVAIRMPKHPVALRLIEGSGCLIAAPSANTSGKPSPTEASHV
                     ALDMDGRIPMILDGGPVGIGIESTIVDLSESTPMILRPGYITQEMLQEVLGEEVRMDP
                     GIIASDSARKPKAPGMKYKHYAPKADLVLVEGETDKVVARINELVREKQQLGQKVGVI
                     ATDETFSRYEADHVVSIGAREDEDAIARHLYKILREFDDWNVDAIYSESFATPRIGQA
                     IMNRLLKAAGHQVIPV"
     gene            2896..3312
                     /locus_tag="ACZO9R_00485"
     CDS             2896..3312
                     /locus_tag="ACZO9R_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013607.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase/protein-tyrosine-phosphatase
                     family protein"
                     /protein_id="HIR0911580.1"
                     /translation="MAAGILQECNLKHPVEIFARGLVVLFPEPLNQKAETVMISNGIN
                     MDGFMSTQLTEEDFSEDALVLVMEHSQREKILEKYGHADPENVYVLTELVGDELEIID
                     PYGGTLQAYGLCYETMRKTILKLVNLLNESEDNGTD"
     gene            3345..3974
                     /gene="upp"
                     /locus_tag="ACZO9R_00490"
     CDS             3345..3974
                     /gene="upp"
                     /locus_tag="ACZO9R_00490"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR0911581.1"
                     /translation="MSKVVVMDHPLIQHKIGIIRREETGSKDFRTLVSEIAMLMCYEA
                     TRDLKLTDVEINTPICKTTVKELKGKKLAVVPILRAGLGMVDGMLNMIPAAKVGHIGL
                     YRDPETLEPVEYYCKLPADCANREVFVVDPMLATGGSSVAAIQMLKDKGVKNIRLMCI
                     IAAPEGVKKMQEAHPDVDIYIGALDDHLNEHGYIVPGLGDAGDRIFGTK"
     gene            4017..4502
                     /locus_tag="ACZO9R_00495"
     CDS             4017..4502
                     /locus_tag="ACZO9R_00495"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR0911582.1"
                     /translation="MSGKRTDYISWDEYFMGVATLSGMRSKDPNTQVGACIVSEEHKI
                     LSMGYNGFPTGCSDDDFPWAREGEPLENKYFYTTHSELNAILNYRGGSLEGATMYVTL
                     FPCNECAKAIIQAGIKRLVYDSDKYNGTPSTIASRKMLSAAGVDIQKYEHTGRIVTIQ
                     L"
     gene            4702..4953
                     /locus_tag="ACZO9R_00500"
     CDS             4702..4953
                     /locus_tag="ACZO9R_00500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR0911583.1"
                     /translation="MKNQRKVFQSLTLILQFGLNMIVPIVMCTLLGAWLGKKYGMPII
                     TVPLFIMGALAGFNNIFKMAKKIYGQGDEGKRDHVEKTK"
     gene            4934..5368
                     /locus_tag="ACZO9R_00505"
     CDS             4934..5368
                     /locus_tag="ACZO9R_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911584.1"
                     /translation="MLRRLNDALPGLVLGIIVYGAAVFLAGIWFVPDKLRFTTGLAIG
                     IALACGMAVNMAVVLRDAVEIYGEAKARSKIIARSITRYLVVVIVFFVMMKWELGSLP
                     AAFIGVLGLKISAYLQPLAHRFISKLGGRGDVPSDSRENEAL"
     gene            5399..6118
                     /gene="atpB"
                     /locus_tag="ACZO9R_00510"
     CDS             5399..6118
                     /gene="atpB"
                     /locus_tag="ACZO9R_00510"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR0911585.1"
                     /translation="MSSGADNIDFMIHGIFSYEIFGHTVWITTSHVCILIVLLLMLGL
                     AIAANRTMKHAKEIPEGFQNVLEIMVEKLDAMVYGTMGKVAAPKFVNYISTIFVFILM
                     SNISGLFGLRPPTADYGTTLALGLITFALIHITQFKNLPPKQIWKDMCSPLPPWLPIW
                     FPINLISEIAVPISLSLRMFANVLSGTVMMALVYGLLGKIAIIWPAALHVYFDLFSGA
                     IQTYVFCMLTMTYISQNYETE"
     gene            6158..6412
                     /gene="atpE"
                     /locus_tag="ACZO9R_00515"
     CDS             6158..6412
                     /gene="atpE"
                     /locus_tag="ACZO9R_00515"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR0911586.1"
                     /translation="MNITGQDLILACSAIGAGLAVIAGIGPGIGQGIAAGHAAAAVGR
                     NPGAKGDIMSTMLLGQAVAETTGLYGLLIAMLLLFVKPLV"
     gene            6465..6980
                     /gene="atpF"
                     /locus_tag="ACZO9R_00520"
     CDS             6465..6980
                     /gene="atpF"
                     /locus_tag="ACZO9R_00520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR0911587.1"
                     /translation="MTRLFNLDFQLLHDVVLMAIAVFFLFMMMSKLLFNPVRKMLADR
                     REKIAGELSDAANDKKDAAALKADYEARLKNIDKEAEQILSEARQKAQKNAARIESEA
                     KEEAARIIQRANEEAELSKKRAMDDVKQEMITVASMMAAKVVAANIDASVQDALVEET
                     LKEMGDSTWQS"
     gene            6968..7534
                     /gene="atpH"
                     /locus_tag="ACZO9R_00525"
     CDS             6968..7534
                     /gene="atpH"
                     /locus_tag="ACZO9R_00525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR0911588.1"
                     /translation="MAKLVSKVYGDALFEVAVESGRLDEIWEQTRAMQTALEENPELF
                     ALMRHPKIVKEEKVKIIESIFTGEVCAELVGLLRMIVEKDHFAQIGKVLAYFDDQVKE
                     YKSIGTAYVTTAMELSDAQKAAVVKRLLETTKYVTFEMHYEVDSAIIGGMMIRIGDRV
                     VDSTVRTKLYDLTRELSKIQLKAGECAP"
     gene            7531..>7811
                     /locus_tag="ACZO9R_00530"
     CDS             7531..>7811
                     /locus_tag="ACZO9R_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAS-barrel domain-containing protein"
                     /protein_id="HIR0911589.1"
                     /translation="MNLKPEEISSVIKEQIKRYAAQLEVADVGTVIQVADGIARIHGL
                     DNAMQGELLEFPGEVYGMVLNLEEDNVGAVLLGSQKNINEGDTVKTTGRV"
BASE COUNT         2105 a         1638 c         2215 g         1853 t
ORIGIN      
        1 cagatggcaa gaagagaagc agcggcagct tcggagaaga aaggaaaaaa gaaaaacgga
       61 gtgtagaatg tgtttgaaaa aggggagctc cataggaagc tccccttcat gctatcatca
      121 cttatgtggg aaaaatcaac cctcgctgat agcgcctgta ggacaggcat cagcacaagt
      181 tccgcagtcc atgcaagaat ctgcatcgat tacgtaatga tcatctccct gagaaatagc
      241 acctgctggg cactcgctct cgcatgttcc acagcttaca caagaatcag aaataacgta
      301 tgccatttta aattccctcc ttaatttgta atcaaaaccc ggctaccctt atagataacc
      361 tttaagacat tctaatacaa aaatacacgg ctttcaagtc aaaaaagccg tgtacttaaa
      421 ttagcacata atgctgttac gaaatatatt ccatgctgga gcttctgcta gaatgttgaa
      481 aaatgataca gtgtcttgga gcggaacggc tcatccggtg tcagcacgat gctcgggaaa
      541 ttgtcgtggt tgattgcgtc cggaaaatgc tgtgtctcaa gacacacgct cgtatggcag
      601 gcatattgcg taccgttctt gccggtgacc tcgatcggct ggtttgccac atacagctgc
      661 atacccggct gatcggtaaa gcatgtcatg cggataccgc tctcctttga ctggagaacc
      721 gctgcctcac ggaatccggt gccattgatg acgaaattgt gatcgtaggt gccgaacttg
      781 tgatactgct cgtagtcgga atccatcagt tttccgatgg tgtgcatgga gcggaaatca
      841 aacggcgttc cctctaccgg aaggaatctg ccggtcggtg cctgtgagtc atcgagctct
      901 gtgatctgat cggcgtcaat gaaaagctcg tgggataaaa cctcactgga gccttcaccg
      961 ttcagattga aatagccgtg gctggtaacg tttaacagcg tatccgcatc cgtggaagct
     1021 tcgtagagaa gggacagctc attgtcctcc gaccagccgt aggttacgga aagtgtgaga
     1081 ttgcccggat aaccttcctc gccgtcgtag gaattcatgg ttaaaatgag tttgtcatcc
     1141 ttgatctttg cgtcccaggg cttggaagcg tagccgatca gtccgccgtg cagatggttg
     1201 gagccgttgt tgatggcaag ctggtattcc ttgccatcca gacagaaatg accgtccttg
     1261 atacgattgg caacacggcc gacaactgcg ccgaggctgg cgttgtcgcg cgcgtaatct
     1321 tccatggaat cgaaaccgag gcagacgtcc gtcatttttc cgtcgcggtc cggaaccacg
     1381 atctttacga gacggcagcc gaaattgatg acttccacat aagcgccgtt tgcgttctcc
     1441 atatggtata tataaatatt ttctcctgtg tttaatgtgc caaaatctga ctttgtaatc
     1501 ttcataggaa actcctttcc atttatatca ggacaaacct aacgcagata attatgccac
     1561 atttgcaaaa acatggcaag tgaattgccc tattttcgga agaatgccca tagcgggcga
     1621 aatgtcaaac ttgcctaaat cgtgaaatta tgcaataatg ttcacgaaag cgaagcggtc
     1681 atgcacgcac tgtaaagcag gtcaggagac agtgcatatg acggccgcaa aggcacaaca
     1741 attgagaggc gaataatatg cagacaaagg ttgttaagat agattctgcg aatatagacg
     1801 atgcagccat gaaagaggcg tgtgatctga tccgcgcggg agagctggtg gcatttccga
     1861 cggagacggt atacggactg ggggcggatg cgctgcaccc ggaggcatcg aaaaagattt
     1921 atgcggcgaa gggaagacct tcggacaatc cgctgattgt acatatttca aagtttgaag
     1981 atctggtatc gatcgcgagg gaggtaccgc cgcaggcgaa gaagcttgcg gacgcgttct
     2041 ggccgggacc gcttaccatg attgtatgga aaaatgacag agtcccttat gagaccacgg
     2101 gcgggatgga taccgtggca atccgtatgc cgaagcatcc ggtagcgctc cggctgatcg
     2161 aggggagcgg atgcctgatt gcagcgccaa gcgctaacac atccggcaag ccgagcccta
     2221 ccgaggcgtc ccacgtggcg cttgatatgg acggcagaat accgatgatt ctggacggag
     2281 ggccggtcgg catcggaatc gagtcgacga tcgttgatct gagcgagtcg acgccgatga
     2341 tcctgcgtcc gggatatatc acacaggaga tgctgcagga agttctcggt gaggaagtcc
     2401 gcatggatcc gggaattatt gcgtcggatt cggccaggaa gccgaaagcg cccggcatga
     2461 agtacaagca ttatgcgcca aaggcggatc ttgtgctggt ggagggcgag acggacaagg
     2521 tcgttgccag aatcaatgag ctggtcagag aaaaacagca gctcggacag aaggtcggag
     2581 tgatcgctac ggatgagaca ttttcgcgct acgaggcgga tcacgttgtc agcatcggcg
     2641 ccagggaaga tgaggatgcc attgccagac acttgtacaa gatcctgcgc gagttcgacg
     2701 actggaatgt ggatgcgatc tactcggaga gttttgccac accgcgcatc ggtcaggcaa
     2761 ttatgaaccg tctgctgaag gcggcggggc atcaggtgat tccggtatag ctacatcagg
     2821 gagagggcag acatttctat gagacaatat aatcgaatca tatttgttgc ggaaagcggc
     2881 acctgcaggg cacctatggc tgcaggaatt ttgcaggaat gcaatttaaa acatccggtg
     2941 gaaatatttg ccaggggact ggtggtttta tttccggaac cattgaatca gaaggcggaa
     3001 actgttatga taagcaatgg tatcaatatg gatggattca tgtccaccca gttgacggag
     3061 gaggattttt cggaggatgc gctcgtttta gtcatggaac acagccagag agagaagatt
     3121 ctggaaaagt acgggcacgc cgatccggag aatgtctatg ttcttacgga actggtagga
     3181 gacgaacttg agatcatcga tccctacggg gggacgcttc aggcatacgg gctctgttat
     3241 gagacgatgc gcaagacgat cttaaagctt gtgaacctat tgaatgaaag tgaagataac
     3301 ggcacagatt aggctctgtg tatcagaaag ggaaggtaca aaaaatgtca aaagtagtag
     3361 ttatggatca cccgctgatt cagcacaaga ttggaattat ccgcagggag gagaccggtt
     3421 ccaaggattt ccgtacactg gtcagcgaga ttgcaatgct gatgtgctac gaggcaacca
     3481 gagacttaaa gcttacggat gtggagatta acacaccgat ctgcaagacc acggtcaaag
     3541 aattaaaggg caaaaagctt gccgttgtcc cgatcttacg tgccggactc ggcatggtgg
     3601 acggtatgct caacatgatc ccggcggcaa aggtcggaca tatcggatta taccgtgatc
     3661 ctgagacatt agagccggta gaatattact gcaagcttcc ggcagactgc gcaaacagag
     3721 aggtgtttgt ggtagacccg atgcttgcga caggaggatc ttcggttgcg gcgattcaga
     3781 tgttaaagga taaaggcgta aaaaatatcc gcctgatgtg cattatcgca gcaccggaag
     3841 gtgtgaaaaa gatgcaggag gcacatccgg atgtggatat ctacatcggc gcgctcgacg
     3901 atcatctgaa cgagcacggc tacattgtac cgggactcgg agatgcggga gaccgtattt
     3961 tcggcacgaa atagaatctg tttgatgcgg cggacacagg tgggagtgat aggaacatga
     4021 gcggaaaacg tacagattat atcagctggg atgaatactt tatgggcgtt gcgaccttgt
     4081 cggggatgcg ctccaaagat ccgaatacac aggtgggagc ctgcattgtc agtgaggagc
     4141 acaagattct ttccatggga tacaatggct ttccgacggg ctgttcggat gacgacttcc
     4201 cgtgggcgcg cgagggcgaa ccgcttgaga acaagtattt ctatacgacg cacagcgaac
     4261 tgaatgcgat tttaaattac cgcgggggaa gccttgaggg cgcgaccatg tatgtcacgc
     4321 tgtttccgtg caacgaatgt gccaaggcaa tcatccaggc aggcatcaag cggctggttt
     4381 acgacagcga caagtataac gggacgccgt caaccattgc gtcgcggaag atgctctctg
     4441 ccgcaggggt ggatatacag aaatacgaac ataccggcag gatcgtcacg atccagctgt
     4501 agaaaaaggg cttttggagc cgccagaaat ggcggctcct tttggtgtca gtaaattcca
     4561 gaatatccat ttatttttaa aacagtttct ttgcgtcggt tgatttttct ttacaaatct
     4621 tttatactta taaaagaatt tgttatggga aaaataatag gcaatggttg cagcagagac
     4681 tgcggtacgg tgaggattta tatgaaaaat cagcgaaagg ttttccagtc tttgacactg
     4741 attctgcagt ttggactgaa catgatcgtg ccgatcgtga tgtgcacgct gctcggtgcg
     4801 tggctgggga aaaaatacgg aatgcccatc ataacagtgc cgttgtttat catgggcgca
     4861 cttgccggat ttaataatat atttaagatg gctaagaaga tctacggaca gggagatgag
     4921 gggaagcggg atcatgttga gaagactaaa tgatgcattg cccggtcttg ttctgggaat
     4981 tattgtatat ggggcggcgg tttttctggc gggaatctgg ttcgtgccgg acaagctgcg
     5041 atttactacc ggacttgcca taggcattgc ccttgcctgc gggatggcgg tcaatatggc
     5101 ggtggttctg cgggatgcgg ttgagatcta cggggaggcg aaagcccggt cgaagattat
     5161 cgcccggtcc attaccaggt atctggtggt cgtcattgtg tttttcgtga tgatgaagtg
     5221 ggagcttggc agtctgccgg cagcatttat tggtgttttg ggattgaaga tatcagcgta
     5281 tctgcagccc cttgcacata gattcatttc aaagcttgga ggaagaggtg atgtgccttc
     5341 ggacagcaga gaaaatgaag ctctataagg aggtgaaatt gtgaataacg caattttgat
     5401 gtcttctggt gccgataata ttgacttcat gatacatggg attttttcct atgagatctt
     5461 tggtcatacg gtctggatta caacatcgca cgtctgcatt ctgattgtgc tgctgctgat
     5521 gttggggctg gcgatcgcgg caaatcggac gatgaaacat gcaaaggaaa ttccggaggg
     5581 ctttcaaaat gttctggaga tcatggtaga gaaactggat gcgatggtat atggaacaat
     5641 gggaaaagtt gcggcgccga aattcgtgaa ctacatcagc acgatttttg tgtttatact
     5701 tatgagcaat atttccggtt tgttcgggtt gagaccgccg actgccgact acggcacaac
     5761 gctggcgtta ggtctcatca catttgctct catccatatc acgcagttca agaatctgcc
     5821 gcctaagcag atctggaaag atatgtgttc tccactgccg ccgtggctgc cgatctggtt
     5881 cccaatcaac ctgatcagcg agatcgcagt accgatctct ctttccttac gtatgtttgc
     5941 gaacgtactg tcaggaacgg tcatgatggc gcttgtatac ggactgcttg gcaagatagc
     6001 gattatctgg ccggcggcgc ttcatgtata ctttgatttg ttctcgggag caattcagac
     6061 atacgtattc tgtatgttga cgatgactta catcagtcag aactatgaga cggaatagac
     6121 gtcgaaacca aaaagattat tttaggaggg tatcagtatg aatatcacag gacaggattt
     6181 aattttagca tgctcagcaa tcggtgcagg tctggcggtt atcgccggta tcggacctgg
     6241 tattggacag ggtatcgcag caggacatgc agctgcagca gtaggaagaa acccgggcgc
     6301 aaagggtgac atcatgtcta ccatgttact tggccaggcg gttgcggaga cgacaggtct
     6361 ctatggtctt ttgatcgcaa tgctcctgct tttcgtaaaa ccgttggtat aggaagagac
     6421 ggagagaacg aatataacgg aaaggaggcc ggaccttgga agagatgacc agactcttta
     6481 atctggattt ccagctgctc cacgatgtcg tgctcatggc gattgcagta tttttcctgt
     6541 tcatgatgat gtcgaagctg ctgtttaatc ctgtcagaaa gatgctcgcc gacagacggg
     6601 aaaagattgc cggagagttg tcggatgccg cgaatgacaa aaaagatgca gcggcgttga
     6661 aggcggatta tgaagccaga ttgaagaaca tcgacaaaga agctgaacag attttaagtg
     6721 aggccagaca gaaggcgcag aagaatgcag cgcgtatcga gtccgaagcg aaggaagagg
     6781 ctgcccgcat tatccagaga gcgaacgagg aagcagagct ttccaagaag cgcgcgatgg
     6841 atgatgtcaa acaggagatg atcacggttg catccatgat ggctgcaaaa gtggttgcgg
     6901 caaatatcga tgcgtcggta caggatgcgc ttgtggaaga gacattaaag gaaatgggtg
     6961 attcgacatg gcaaagctag tatcaaaggt atacggtgat gcactgttcg aggtcgcagt
     7021 ggagagcggc aggctggatg agatctggga gcagaccaga gccatgcaga ccgcgctgga
     7081 ggaaaatccg gagctctttg cgctgatgcg tcacccgaag atcgtaaaag aagaaaaagt
     7141 aaagatcatc gaatcgattt ttaccggtga ggtgtgtgca gaactggtgg gacttttgcg
     7201 catgattgtg gaaaaggatc attttgcaca gatcgggaag gttttggcgt attttgatga
     7261 tcaggtcaaa gaatataaga gtatcgggac tgcatatgtg acgactgcaa tggaattatc
     7321 ggatgcgcag aaggcagcag tggtgaaacg cttattagag accacaaagt atgtaacatt
     7381 tgaaatgcat tatgaagtgg attccgcgat cataggcgga atgatgattc gcattggcga
     7441 tcgtgtcgtt gactcaacgg tcaggacaaa gttgtacgat ctgacgcgcg aattatccaa
     7501 aatacagttg aaagcaggtg aatgcgctcc atgaatttaa aaccagaaga aataagttcc
     7561 gtcatcaaag aacagatcaa acggtatgca gcgcagttgg aagtagctga cgttggcacc
     7621 gtaattcagg tggctgatgg aatcgcacgt attcacggtc ttgacaatgc aatgcaggga
     7681 gaacttttgg agttcccggg cgaagtatac ggaatggtat tgaacctcga ggaggacaat
     7741 gtgggtgccg tacttctcgg ttcccagaag aacatcaacg agggagatac ggtaaagaca
     7801 acggggcgtg t
//
LOCUS       DBMDQJ010000012         7678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4070_length_7678_cov_1.576349, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000012 DBMDQJ010000000
VERSION     DBMDQJ010000012.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4070_length_7678_cov_1.576349"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="ACZO9R_00535"
     CDS             complement(<1..513)
                     /locus_tag="ACZO9R_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911590.1"
                     /translation="MKESIDCPKLVEQSKKVQKGQHWLVELLVFVTVYIIAAFGEMLI
                     LLPGELVLLNTNTAYQDAAASGDMEQLSEVSVQIAGSDAYAVLKLFSGIAMLLVVVLF
                     CRKFQKRSFEMIGFVKEHALREYAIGLGLGVVFAVVTGGIAVAAGKAKLFTGTAPGML
                     VLFLLGYLIQG"
     gene            complement(758..2452)
                     /locus_tag="ACZO9R_00540"
     CDS             complement(758..2452)
                     /locus_tag="ACZO9R_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HIR0911591.1"
                     /translation="MKKKWWHDKVAYQIYPKSFLDTNGDGIGDLRGIISKLDYLKELG
                     IDIIWLSPIYKSPFVDQGYDISDYYAIAQEFGTMEEFDELLAEAKKRGMYIVMDLVVN
                     HCSDQHEWFKKALADPDGEYADYFYFVKEKDGREPSNYRSYFGGNAWEPVPGTDKYYL
                     HMFAKEQPDLNWENPVVRQKIYDMVNWWLAKGLAGFRIDAIINIKKDTAFPSFPPDGD
                     DGLASCVKMVEEVQGVGALLEDLKHATFEKWDAFTVAEVFNMKEDELPEFIGDDGHFS
                     TMFDFSAHELTYGEHGWYDSKPIEFKKWRDTLFASQLKLQKAGFAANIIENHDEPRGA
                     SRYLPEHARNEAGKKMLGTTSILLRGIPFIYQGQEIGMTNCIRNDISEYDDISTKDQY
                     QVAIDAGCSREEALRCCYENSRDNARTPMQWTDDENAGFTDGTPWLAMNPNYRQINVR
                     EQEARTDSVLAYYRRLVHLRKADAYRETFTYGIFEPAYQEMADVFAYYRVSGESGQRI
                     LVAANFGTDAVSLTLAYPCRQVLLSNLRAESAVETACEKANALTLGSCEVAVIELG"
     gene            2596..3492
                     /locus_tag="ACZO9R_00545"
     CDS             2596..3492
                     /locus_tag="ACZO9R_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0911592.1"
                     /translation="MNRTSIPAPEQKEHARHGEQLFPLQKYITTLDDLHPSVSAHWHE
                     EAEFTRILSGSCTYQIQLQHYDVAAGDLLFIPPTLLHAITVTPRETLCTETYVFHMHF
                     LGASTADICSVQYLTPLATAKLIPPFFFDALHPLHTALDDLFDDMDALHDAAAPGYEL
                     LLKADFLRLIALLLPCCSAADEDTRLHMEYTAKLKTVLEYIDAHYTEELSIADLASLC
                     YFSEYHFMRFFKRFMGTSCLNYIKNLRLEKAAELLGQDGLTPLDASLSAGFHNLSYFY
                     REFRKKYGVTPGDFRGAGAREK"
     gene            complement(4055..4918)
                     /locus_tag="ACZO9R_00550"
     CDS             complement(4055..4918)
                     /locus_tag="ACZO9R_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006775899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0911593.1"
                     /translation="MQKNLQTRFSTRQYMLSRDFELYYYNEHYTSKVDTHTHDYYEFY
                     FFMEGDVSIEIEGQRYPLRYGDMVVIPPHRRHRAVVHNHAQAYRRFVFWVSREYASRL
                     MELSPAYGYLMQRAEVSGKNVFHSDVITFNATQFKIFQLIEEMQMERFGREARIPLLV
                     NDLILHLNRMVYEEENPAKVKEQDLYQNLIYYIEEHLEEELSLEKLAGAFFVSRYYVA
                     HVFKEQAGISVHQYILKKRMQASREAILGGENISQVYERFGFRDYSSFYRAFRREYGM
                     SPKEYREERLR"
     gene            5144..6226
                     /gene="uxuA"
                     /locus_tag="ACZO9R_00555"
     CDS             5144..6226
                     /gene="uxuA"
                     /locus_tag="ACZO9R_00555"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR0911594.1"
                     /translation="MEMTLRWYGSKFDTVTLEQIRQIPGVTGVITTLYDTAPGEVWSR
                     ERIAALKAEVAAAGLHISGIESVNVHDAIKTGAPDRDLYIDHYIKTLENLGKEDIHLV
                     CYNFMPVFDWTRTELARKRPDGSTVLAYTQEAVDALDPEKMFESIAGDTNGSIMPGWE
                     PERMAHIKELFAMYKDIDDEKLFANLKYFLERIMPVCDKYDINMAIHPDDPAWSVFGL
                     PRIIINKKNILRMMSMVDNPHNGVTFCSGSYGTNLENDLPDMIRSLKGRIHFAHVRNL
                     KFNSPTDFEEAAHLSSDGTFDMYEIMRALYEIGFDGPIRPDHGRMIWGEVAMPGYGLY
                     DRALGATYLNGLWEAIEKGAARYEAK"
     gene            6213..>7678
                     /locus_tag="ACZO9R_00560"
     CDS             6213..>7678
                     /locus_tag="ACZO9R_00560"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HIR0911595.1"
                     /translation="MKLNNKGLENKNAWEAAGYALPQYDREAVAARTKQNPFWIHFGA
                     GNIFRAFQANVVQDLLNKGDLDRGLVVAEGYDYEIVEKMNHPHDDYSILVTLKANGTV
                     EKTVVGSVMESLALDSEDDAQFSRLKEIFSADSLQMATFTITEKGYNLYGSDGNFMPA
                     VAADMAAGPQKPASYIGKVASLLYARFLAGEKPIAMVSMDNCSHNGDKLAAAITAFAE
                     GWVKNGLADEAFETYVKTSGKVSFPWTMIDKITPRPDASIEEILKKDGVEELDPVVTS
                     KNTYVAPFVNAEECQYLVIEDNFPNGRPALEKGGLIFTTRETVDKVEKMKVCTCLNPL
                     HTALAVYGCLLGYNLISEEMKNPSLKKLVEVIGYKEGLPVVINPGILDPKEFIDTVLN
                     VRIPNPFMPDTPQRIATDTSQKLAIRFGETIKAYAAAPSLNVADLKLIPLVFAGWLRY
                     LMGVDDNGNAFTPSPDPMLAEASSYVADVKLGEAFDAK"
BASE COUNT         1819 a         2227 c         1754 g         1878 t
ORIGIN      
        1 cccctggatc agatatccca ggagaaacag caccagcatc cccggcgccg ttccggtaaa
       61 cagcttcgct tttcccgccg ccaccgcgat accccccgtg actaccgcaa ataccacccc
      121 aagtcccagt ccaatggcat attcccgcag cgcgtgctcc ttcacaaaac cgatcatctc
      181 aaagctgcgc ttctggaact tcctgcagaa taagacgacc accagaagca tggcaattcc
      241 ggaaaaaagc tttaacaccg cgtaggcatc cgaacccgca atctgcaccg acacctccga
      301 cagctgttcc atatccccgg aggcagccgc atcctgatat gccgtgttcg tattcagcag
      361 cacaagctct cccggcagca gaatcagcat ctctccaaat gccgcaatga tgtacaccgt
      421 cacaaacacc agcaattcca ccagccagtg ctgccccttc tgtacctttt tcgactgctc
      481 aacgagcttc ggacaatcta tgctctcttt catctcaatc tcccatcctg cgtgtgggct
      541 tctgtctgcc cgctgcgaca tttcatttta cggaaaactg cctgtttccg ccgtttttct
      601 gcacttatca taacataaaa aatggagcga tgcaaaaatt tcctgcaccg ctccacacgc
      661 attttccggc tcatttcttc cgccggctga cgcttattcc ggctcacttc ttccgccggc
      721 tgacttccat cccgatccgg ttactaacgc cggattccta tcccagctcg attaccgcca
      781 cttcacagct tccgagtgtc agcgcgttcg ctttttcaca tgccgtctcc accgcgctct
      841 ctgctctcag attcgacagc agtacctgcc tgcacggata ggcaagcgtc agggaaaccg
      901 catccgttcc aaagtttgct gcaaccagta tgcgctgccc actctccccg gacacacggt
      961 aatacgcaaa tacatccgcc atttcctggt aagccggctc gaagataccg taggtgaatg
     1021 tttcccggta ggcgtccgcc ttgcgcagat gcaccagtct ccggtaatag gcaagcaccg
     1081 aatccgtccg cgcctcctgc tccctgacat tgatctgccg gtaattcgga ttcattgcca
     1141 gccacggcgt tccgtccgta aatccggcat tctcatcatc cgtccactgc atcggcgttc
     1201 tggcattgtc cctgctgttc tcgtagcagc agcgcagtgc ctcctccctg ctgcagcccg
     1261 cgtcaatggc aacctgatac tgatccttcg tgctgatgtc gtcatactcc gaaatgtcgt
     1321 tccggatgca atttgtcatg ccgatctcct gtccctgata gataaacggg atgccgcgga
     1381 gcagaatgga tgtggttccc agcatctttt tcccggcttc atttcttgca tgctccggca
     1441 gatagcgact cgccccgcgc ggctcgtcgt gattctcgat aatgttcgcc gcaaatcccg
     1501 ccttctgcaa ttttaactgg gatgcaaaca gcgtatcgcg ccattttttg aattcgattg
     1561 gtttgctgtc ataccagccg tgttctccgt aggtcagctc atgtgcggaa aaatcaaaca
     1621 tggtggaaaa atgcccgtca tccccgatga actccggcag ttcgtcctcc ttcatgttaa
     1681 aaacttccgc cacggtaaat gcatcccatt tttcaaaggt tgcatgtttt aagtcctcta
     1741 aaagcgcgcc gactccctgc acttcctcaa ccatcttcac acaggaggca agtccgtcgt
     1801 ctccatccgg cgggaatgac gggaatgccg tgtctttttt gatgttgatg attgcatcga
     1861 tgcggaatcc ggcaagtccc ttcgccagcc accagttcac catatcgtag atcttctgac
     1921 ggacaacggg attttcccag tttaaatccg gctgctcttt tgcgaacatg tgcaggtaat
     1981 atttgtccgt tcccggcacc ggctcccacg catttccgcc aaaatacgag cggtaattgc
     2041 tcggctccct gccgtctttt tccttcacaa agtagaagta atctgcatat tcgccatccg
     2101 gatctgccag cgcctttttg aaccattcat gctggtcgga acagtggttc accaccagat
     2161 ccatcacaat gtacatgcct ctcttttttg cctcggcaag cagctcgtca aactcctcca
     2221 tcgttccgaa ttcctgcgcg atcgcgtagt agtcggaaat gtcatacccc tgatccacaa
     2281 agggagattt ataaatcgga gagagccaga tgatgtcaat gccaagctct ttcaaatagt
     2341 ccagcttcga gataattccc cgcagatccc cgattccgtc cccgttcgta tccagaaagc
     2401 tcttcggata aatctgatat gccactttat cgtgccacca ttttttcttc attgcacatt
     2461 ctcctttttc ccaatacggt ttttcttttc ctttttgtat gatactgtta ttataataag
     2521 agaaatccgt aatatcttgc actatatttt cttttttatt gcacaatact gctatttaat
     2581 atcaggggaa cgcacatgaa caggacatcc attccggcgc cggagcaaaa ggaacacgcc
     2641 cggcacggtg aacagctttt tccactgcaa aaatatatca ccacactcga cgatctgcac
     2701 ccgtccgtca gcgcacactg gcacgaggaa gcggaattta cgcgcattct ctccgggagc
     2761 tgcacctatc agatccagct gcagcactat gacgttgccg cgggcgatct cctcttcatc
     2821 cctccgacgc tgctgcacgc aatcaccgtg acacccagag agacgctttg caccgagact
     2881 tatgtttttc acatgcattt cctcggcgca agcaccgcag atatctgctc cgtgcagtat
     2941 ctgacgccgc ttgcgacggc aaagctgatc ccaccttttt tctttgatgc ccttcatccg
     3001 ctgcacactg ccttagacga tctgttcgac gatatggatg ccctgcacga cgccgccgca
     3061 ccgggctatg aactgctgct gaaggctgat tttctgcgcc tgattgccct gcttctcccc
     3121 tgctgcagcg cagcggatga ggacacacgg ctccacatgg aatataccgc caagttaaaa
     3181 acagttctgg agtatattga cgcccattat acggaggagc tttccatcgc cgatctggct
     3241 tccctgtgct atttcagcga gtatcatttc atgcggtttt tcaagcgctt catgggtacc
     3301 tcctgcttaa actacatcaa gaacctgcgt ctggagaagg ctgccgagct gctcgggcag
     3361 gacgggctta ccccgctgga cgcctccctg tccgccggct tccacaacct gtcctacttc
     3421 taccgggaat tccggaaaaa atatggcgtg accccggggg atttccgcgg agccggtgcc
     3481 cgggagaagt agactttata ataggaaata tcattccgga gcgaagcagc gggacggagt
     3541 ctttgaaaca aaatgcaaga gtttcttccc tgccgctttc ggcaattcat tccgtgtctt
     3601 tgccacgctt gaacaatccg cgaatgtgtc cgacaaaccc gcagacaatg agcggacttc
     3661 ctagcagcac taagcacacc tcttcacaga catgataaaa ccgcgcgagc cgaaactcgt
     3721 tgctttctgc tattaaaaac aaggcagaaa gcagctcaaa catcggtctc gcgcggttat
     3781 gttttgctca tcggtgtgca aagtgctgca cgtaagtcct aaaattgtct gcggcttgcg
     3841 gacacattcg cggagtgctc caagcctgga aagacgcgga tcatcgcagt tgtcgaaagc
     3901 ggctgggtta gaaagtccgt attttgtgat cagactccgc cccgctgctt cgctccggaa
     3961 tgtgattgtc tgttataaat ccccgtaaag atcacttctc cccggcaggc tccgtgaaaa
     4021 ccgcccgggg catgccctat tttccggtca gcggctaccg caggcgctcc tcgcggtact
     4081 ccttcggcga catgccatat tcccggcgga acgcgcggta aaagctggaa tagtcccgga
     4141 acccgaaccg ctcatacacc tggctgatgt tctcaccgcc taaaatcgcc tcccgggacg
     4201 cctgcatcct tttctttaaa atatactggt gaaccgagat tcccgcctgt tccttaaaga
     4261 cgtgcgccac ataataacgg ctcacgaaaa aggctcctgc cagcttttcc aacgacagtt
     4321 cctcctccag atgctcctct atataataga tcagattctg atagagatcc tgctccttta
     4381 ccttggcggg attttcttcc tcgtacacca tccggttcaa gtgcaggatc agatcgttga
     4441 ccagcagcgg tatccgcgcc tccctcccaa aacgctccat ctgcatctcc tcaatcagct
     4501 gaaagatttt aaactgcgtg gcgttaaatg taattacatc actgtgaaac acatttttcc
     4561 cggacacttc cgcgcgctgc atcaggtagc cgtatgccgg agaaagttcc atcagccggc
     4621 tggcatattc ccggctgacc cagaacacga accggcggta tgcctgcgca tggttgtgca
     4681 caacggcacg gtgcctgcgg tgcggaggaa ttaccaccat atccccatag cggagcgggt
     4741 atctctgccc ctcaatctca atcgagacgt ccccctccat aaaaaaatag aactcataat
     4801 aatcgtgtgt gtgcgtatcc acttttgacg tgtaatgttc attataataa tatagttcaa
     4861 aatcgcggga cagcatgtac tgtctggtgc tgaatctggt ctgtaaattt ttctgcatgc
     4921 gctatttcct cttcgggcac tcctttcatg ccgccattga tccgttgctt tcctttttcg
     4981 tcttatattt tttcaaattc ttccctgatt atagcagaag atagcaactt ttgcaatgta
     5041 tgcaacaagt ctttcaattt gcttttggag cggattttat ataatgaaat catccaatat
     5101 gacacttcct taacggagca gaaaagaacg gaggttacac actatggaaa tgactttaag
     5161 atggtacggt tccaaattcg acaccgtcac cttagagcag atcagacaga ttccgggcgt
     5221 gaccggagtc atcaccaccc tctacgatac cgcaccgggt gaagtctgga gcagagagcg
     5281 catcgccgca ttaaaggcgg aagttgccgc cgcaggactt catatttctg gtatcgagag
     5341 cgtcaacgtc cacgacgcca tcaaaaccgg cgcaccggac cgtgatctct acatcgatca
     5401 ctacattaag acactggaaa atctcggaaa agaagatatc catctcgtat gctataactt
     5461 catgcctgtt ttcgactgga cccgcacgga actcgccaga aaacgtccgg acggttctac
     5521 tgtcctcgct tacacccagg aggctgtgga tgccctcgat ccggagaaaa tgttcgagtc
     5581 catcgccggg gataccaacg gcagcatcat gccgggctgg gagccggagc gcatggcgca
     5641 catcaaagag ctttttgcca tgtacaagga cattgacgat gaaaaattgt ttgccaacct
     5701 gaagtatttt ctggagcgca tcatgccggt gtgcgacaag tatgacatca acatggcaat
     5761 ccatccggac gatcctgcct ggagcgtatt tggtcttccg cgcatcatca tcaacaagaa
     5821 aaacattctc cgcatgatga gcatggtcga caatccgcac aacggcgtaa ctttctgctc
     5881 cggttcctac ggaaccaacc tggagaacga tctgccggat atgatccgtt ccttaaaggg
     5941 gcgaatccat tttgcccatg tgcgcaatct gaaattcaac agcccgacag actttgagga
     6001 agccgcacat ctctcctcag acggcacttt tgatatgtat gaaatcatgc gtgccctcta
     6061 cgagatcggg tttgacggtc cgatccgtcc ggaccacgga cgcatgatct ggggcgaagt
     6121 cgctatgccg ggttacggcc tttatgaccg cgcacttggc gccacctacc tgaacggact
     6181 ttgggaagca atcgagaaag gagctgcacg ctatgaagct aaataacaaa ggattagaga
     6241 ataagaacgc atgggaagct gccggttacg cacttccgca gtatgacaga gaggcggttg
     6301 ccgccagaac aaagcagaat ccgttctgga ttcatttcgg agccggcaac attttccgtg
     6361 ccttccaggc aaatgtggta caggatcttt taaacaaggg agatcttgac cgcggtcttg
     6421 tcgttgccga aggctacgat tatgaaattg tcgagaaaat gaatcatccc catgacgact
     6481 acagcattct cgtcacatta aaagcaaacg gcaccgttga gaaaaccgtt gtcggcagtg
     6541 tgatggagtc tctggcactc gattctgagg atgatgcaca gttctcccgc ctgaaggaga
     6601 ttttctccgc agactccctg cagatggcaa cttttaccat cacagaaaag ggttacaacc
     6661 tctacggttc tgacggaaac ttcatgccgg cagttgccgc agatatggca gcaggtccgc
     6721 aaaagccggc cagctatatc ggaaaggtcg catctcttct gtatgcacgt ttccttgcag
     6781 gtgagaagcc gatcgccatg gtaagcatgg ataactgctc ccacaacgga gataaactcg
     6841 cggctgccat cacagctttt gcagagggct gggtaaagaa cggtcttgcc gatgaggcat
     6901 ttgagactta tgtaaagact tccggcaagg tatccttccc gtggacgatg atcgataaga
     6961 tcaccccgcg cccggacgct tccatcgagg agatcttaaa gaaagacggc gtcgaggaac
     7021 tggatccggt tgtcaccagc aaaaacacct atgtggcgcc ttttgtcaac gcagaggaat
     7081 gccagtatct cgtcatcgaa gacaacttcc cgaacggccg tccggctctg gaaaagggcg
     7141 gtctgatctt taccacacgg gagaccgtcg acaaagtcga gaaaatgaag gtctgcacct
     7201 gcttaaaccc gctccacacc gcgcttgctg tctacggctg cctgctcggc tacaacttaa
     7261 tctccgagga gatgaagaat ccttctttaa agaagcttgt tgaagtgatc ggatataaag
     7321 agggactccc ggttgtcatc aacccgggca ttctcgatcc gaaagaattc atcgataccg
     7381 tattaaatgt ccgcattcca aatccgttta tgccggacac accgcagcgt attgcaaccg
     7441 atacttccca gaagctggca atccgtttcg gtgagaccat caaagcctat gcggcagctc
     7501 cttctttgaa cgttgccgac ttaaagctga tcccgcttgt atttgccgga tggctgcgct
     7561 acctgatggg tgttgacgac aacggcaacg cgttcacccc aagcccggat ccgatgcttg
     7621 cagaagcgtc ctcctatgtc gcagacgtaa aactcggcga agcgttcgat gcaaagaa
//
LOCUS       DBMDQJ010000013         7650 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4091_length_7650_cov_1.559147, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000013 DBMDQJ010000000
VERSION     DBMDQJ010000013.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_7650_cov_1.559147"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            192..713
                     /locus_tag="ACZO9R_00565"
     CDS             192..713
                     /locus_tag="ACZO9R_00565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0911596.1"
                     /translation="MQSLETLTERDFDSVWEIMEESFPRDERRSREAQRKILDVSWYH
                     LYGYRAHGELMAFLAVWEFDTFLFVEHFAVRKSCRNGGLGAVLLTQLTAQSRKPVILE
                     VELPTGELEARRIAFYERNGFVMNPFEYMQPAMGEDRHAIPLRIMSYPKRLLADEFET
                     MRDVLYCHVYRQK"
     gene            complement(716..1483)
                     /locus_tag="ACZO9R_00570"
     CDS             complement(716..1483)
                     /locus_tag="ACZO9R_00570"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HIR0911597.1"
                     /translation="MKLISWNVNGLRACMQKGFAEFFTASDADIFCIQESKLQEGQID
                     FAPEGYYAYWNYAQKKGYSGTAIFTRKEPLSVHYGIGIPQHDTEGRVITLEFPDFYMV
                     TCYTPNSQNELARLSYRMEWEDAFLSYLKGLDRNKPLILCGDLNVAHEEIDLKNPKTN
                     RKNAGFTDEERTKMTALLSAGFTDTFRHFYPDTEGIYSWWSYRFHAREKNAGWRIDYF
                     ITSSALDDRLVDAGILTDVTGSDHCPVTLTVDLPFCP"
     assembly_gap    1577..1586
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1606..2328
                     /locus_tag="ACZO9R_00575"
     CDS             1606..2328
                     /locus_tag="ACZO9R_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HIR0911598.1"
                     /translation="MAVWNPWHGCKKISAGCDNCYVYEKDAMYGKNASVIRRTANFDL
                     PVKKNRRGEYKLLPQEEPVYVCMTSDFFLPEADEWRSEAWAMIKERQDLSFVIETKRE
                     HRFFKALPGDWGDGYENVTILCSVEIQRRADDRIPAFLKLPVRHKGILCEPLLEKLVL
                     DAYLKTGEIAQVLCGGEQGADARVCDFAWVLELMNQCVNCGVAFRFLRTGTFFRKGNR
                     IYRIAEEEQEEQARRAGVDYTP"
     gene            2348..4027
                     /locus_tag="ACZO9R_00580"
     CDS             2348..4027
                     /locus_tag="ACZO9R_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025925.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR0911599.1"
                     /translation="MNVQYAKEVIELPAGSGGKAETLLMRLIRAGYYVDAPCGGKGTC
                     GRCRVRFVSEAPQPTANERRLLTEEERSSGVRLACEVRVAEACSLQLPVSREQEIDVL
                     VTADAADGAIPSRTVDEGIPGQTAGAIPGERDCAAMHNREGAAKIWGHSGKQRCGAAV
                     DIGTTTLAATLYDLTERKRIAAASSVNHQRAYGADVLSRIQAANEGAAGELRLSICRD
                     IDALLAGLVADAGIPDDAVEELVIVGNTTMCHLLRGLSCAGLGAAPFTPEDLSLWEGS
                     GAELALSDRWSVSVTILPGISAFVGADIVAGIYALEMDRKKNALLLDIGTNGEMAAGG
                     ADGFLVTSAAAGPVFEGGNISCGVAGVPGAVTSLVLERDPAAIGGIRTVELSTIAGET
                     PIGLCGTGIIDAVGEAVRLGLIDENGTFEEPWFTDGMPVAEGRFRFRQEDVRQVQMGK
                     AAIRAGIETLLAEYGGGDPEEILIAGGFGCRMDEEQTIRIGLFPEQFRGHITMIGNSA
                     LAGAQRYLLDDRTAAKQRLASITGHAQELSLAMHPKFNELYLDEMFFDTDR"
     gene            4045..5412
                     /locus_tag="ACZO9R_00585"
     CDS             4045..5412
                     /locus_tag="ACZO9R_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0911600.1"
                     /translation="MEQKNDFLGRDPIGRLLWRMSLPAIVAQLINLLYNLVDRVYIGH
                     MPEDGALALTGVGVCLPIIMIVSAFAALVSNGGAPRASMAMGKGDYDEAEKILGNSVA
                     LQIVISAVLTVLLLLFYRPLLLTFGASENTIGFASDYMKIYAAGTVFVQLTLGMNAFI
                     TAQGFARTGMLSVMIGAGCNIVLDPVLIYGFGMGVRGAATATVISQAVSTVWVLSFLT
                     GKKTVLKIRKKYLRMEAGVVLPGLALGLAPFIMQASESVITVCFNASLLKYGGDVAVG
                     AMTILSSAMQFSMLPMQGLGQGAQPITSYNYGARNAERVKRTFRLLLTTSLCYSMAVW
                     LLIMLFPQMFAMIFSSNAELIAYTKWALRIYCAAMGIFGAQIACQMTFISIGNAGASI
                     IVAIMRKFVLLLPLIYLLPHLVKNQTMGVYLAEPAADVIAVSFTLVLFFFQFKKAIAV
                     IEKPQ"
     gene            5577..>7650
                     /locus_tag="ACZO9R_00590"
     CDS             5577..>7650
                     /locus_tag="ACZO9R_00590"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HIR0911601.1"
                     /translation="MGSRDKVLIVDDSELDRIALKKMLKDAYEIVEAVDGAGALRILE
                     SQKEFDAISAIILDLVMPQMDGFEFLREYRKVDSYRRIPVIVASVERDVKTEKACLAL
                     GARDFIGKPYDPQIVRFRVENAVRSSEQQLSRELRYRADYDMLTGIYNKTHFFEATRV
                     LLKRHPEETYAFLRLDIEKFQLVNSFFGSSSGDGLLQYIAKEIRKFAGDTEQIAYGRI
                     EADIFGICMPYRGEETMLELVRYMRMRLGQYQLAFDVVPAIGIYVIRERDLSVDAMYD
                     RANLAAKQCKGNYIENYAFYVDEMREDIVREQQIVNHMRHALDEEEFVLYLQPKYSLQ
                     DNRICGAEVLVRWAKPEGGMISPGEFIPIFERNGFITKLDYYVWEKTCKLLAGWIAEG
                     KKPSPVSVNISRVSLYNPRLVEAICGLTDRYHIPRELLELELTESAYTTNPKAIKETM
                     ERLQKEGFSILMDDFGSGYSSLNVLKDIAVDVLKIDMKFLSDTDQEGRSQNILASVVR
                     MAKWLNLPVIAEGVERREQVDFLHSVGCEYVQGFYFARPMPVSDYEKLVSEKKDELQE
                     KYQKDEIGRDMLWNATSQMGSVFSGMMEPAVIYEYEKEQNRIDALRVNNAYYDMFGYG
                     DINSSGGLVMEKQLSDESRKILLGTFEDIAKSKETAACEFLRTSKSGRSIWVHLKLKY
                     VTSVGEKHI"
BASE COUNT         1847 a         1655 c         2410 g         1728 t
ORIGIN      
        1 acagacccgg catcgtttat gattatattg gagtccaatg aagtgcacgg agagggcttc
       61 cgcaccatca ggatcggaga gggtgagacg caggcaaaga acagtgcggt ttgattccat
      121 aggaaatttg tgctataatc ttgttttatg gtataaaagt aagtaagaaa gaatcagccg
      181 ggagagagaa catgcagagt ttagagacac tgacagaacg ggattttgac agcgtatggg
      241 agattatgga ggagagtttt ccgcgggacg agagacgatc cagggaggcg cagagaaaga
      301 tactggatgt ctcatggtat cacctgtacg ggtaccgtgc acatggggaa ctgatggcgt
      361 ttctggcagt gtgggagttt gatacattcc tgtttgtgga gcactttgcc gtgagaaaat
      421 catgccgaaa cggcggactt ggagcggtac ttctgacaca gctgacggcg cagagcagaa
      481 agccggttat actggaggtg gaactgccga ccggtgaact ggaagccaga cggattgcat
      541 tttatgagag aaacggattc gtgatgaatc cgtttgagta tatgcagccg gcgatgggag
      601 aggacagaca tgccatcccg cttcggatca tgtcttatcc gaagcggctt ttggcggatg
      661 aatttgagac catgcgggac gtgctttact gtcatgtcta tcggcagaaa tagtgctatg
      721 gacagaatgg cagatcgacg gtaagtgtta ccggacagtg gtcggagccg gtgacatcgg
      781 tgagaattcc tgcatccaca aggcggtcat cgagtgcgga tgaggtgatg aagtagtcga
      841 tacgccatcc ggcgttcttc tctctggcgt gaaagcggta ggaccaccag gagtagatgc
      901 cctcggtatc cggataaaaa tggcggaacg tatcggtgaa gccggcggat aaaagagccg
      961 tcatttttgt gcgctcctcg tcggtaaaac cggcgttttt ccggttggtt ttcggattct
     1021 taaggtcgat ctcttcgtgg gcgacattga gatcgccgca gagaatcaga ggcttgttcc
     1081 ggtcaagacc ttttagatat gagagaaatg catcttccca ctccatgcgg taggagagcc
     1141 ttgccagttc attctgggaa tttggcgtgt agcaggtgac catataaaaa tccgggaatt
     1201 ccagcgtaat cacacgtccc tcggtatcgt gctgtggaat gccgatgccg taatgcaccg
     1261 acagaggttc ctttctggta aaaatggcgg tgccggagta gccctttttc tgcgcataat
     1321 tccagtaagc gtaatagccc tccggtgcaa aatcaatctg cccctcctgc agtttggatt
     1381 cctggataca gaaaatatca gcatcacttg ctgtaaaaaa ttctgcgaag cctttctgca
     1441 tgcaggcgcg cagaccgttg acattccatg agatcagttt catagacaat tcctttctgt
     1501 ggctgcccgg atgtggcagc ggttttactt tttccagatt tgtgataagt ataccatccg
     1561 gcggacaaaa aggaaannnn nnnnnnagaa ggaggcgcgt ttcgtatggc ggtctggaat
     1621 ccctggcatg gctgcaagaa aatcagtgca gggtgcgaca actgttatgt atatgaaaaa
     1681 gatgcgatgt acggcaaaaa tgcgtccgtg atccgaagga cagccaattt tgatctgccg
     1741 gtaaagaaga accggagagg agaatacaag ctgctgccac aggaggaacc ggtctatgtg
     1801 tgcatgacgt cggatttttt cctgccggag gcggatgaat ggcggagcga ggcgtgggcg
     1861 atgatcaagg agcggcagga tctttccttt gtgattgaga caaagaggga gcaccgcttt
     1921 tttaaagcac tgcccggcga ctggggagac ggctatgaaa acgtgacgat tctctgttcg
     1981 gtggagattc agagacgcgc ggatgaccgg attcctgcat ttttaaaact gccggtgcgc
     2041 cataagggaa ttctctgcga accgctcctt gaaaaactgg tgctggatgc gtatctaaag
     2101 accggggaga ttgcgcaggt tctctgcggc ggcgaacagg gagcagacgc gcgtgtatgc
     2161 gattttgcct gggtgctgga actgatgaac cagtgcgtga actgcggggt tgcattccgc
     2221 tttttgcgga ccggcacttt tttccggaaa gggaaccgga tataccggat cgcggaggag
     2281 gaacaggaag agcaggcgag gcgggcaggc gtggactaca cgccataacg cggggaggaa
     2341 tgacaggatg aacgttcaat atgcaaaaga agtgatagaa cttccggcgg gatcgggcgg
     2401 gaaggcagag acgctgctga tgcgtctgat aagagcagga tattatgtgg atgcgccctg
     2461 cggtgggaag ggaacctgtg gaagatgccg cgtgcgcttt gtgtcggagg caccacagcc
     2521 gacggcaaat gagcggaggc tgctcacaga ggaagaacga agcagtggcg tgcgcctggc
     2581 gtgtgaagtg cgcgtggcag aggcatgcag cctgcagctt cccgtaagcc gggagcagga
     2641 gatcgatgtg ctggtcacgg cggatgcagc agacggagcc atccccagtc ggacggtaga
     2701 tgaaggtatc cccggtcaga cggcaggagc catccctggt gagagagatt gcgcggcgat
     2761 gcacaacaga gagggtgcag ccaagatatg ggggcattcc gggaaacagc gctgcggcgc
     2821 tgccgtggat atcggtacca ccacgctcgc tgcaacgctt tatgacctta cagagaggaa
     2881 acggattgcc gccgcatcct cggtaaatca ccaacgggca tacggcgcgg atgttctgtc
     2941 cagaatccag gcggcaaacg agggggcggc aggggaactg cgccttagca tctgccggga
     3001 tattgatgcg ctgcttgcgg gactcgtggc ggatgccggg ataccggatg atgctgtgga
     3061 ggaacttgtg attgtcggaa atacgaccat gtgtcatctc ctgcgcggat tatcctgtgc
     3121 agggcttgga gcggcgcctt ttacgcctga ggatctctct ctctgggagg ggagcggtgc
     3181 agagcttgca ctgtctgacc ggtggagtgt gtcagtgacc attttgcccg gaatctctgc
     3241 tttcgtcgga gcggatatcg tggcgggaat ctatgccttg gaaatggacc ggaagaaaaa
     3301 tgcgttgctg ctggacatcg gcaccaacgg ggagatggca gccgggggag cggatggatt
     3361 tcttgtgact tccgcagcgg cgggaccggt ctttgagggc gggaatatat cctgcggtgt
     3421 tgcgggcgtt ccgggagccg tgacaagcct ggttctggag agagatcctg ccgccatagg
     3481 aggaataaga accgtggagt taagcaccat cgctggtgag acaccgatcg gcttatgcgg
     3541 aaccgggatt attgacgctg tgggagaagc cgtgcggctc ggactcatcg atgagaacgg
     3601 gacgtttgag gaaccctggt ttacggacgg catgccggtc gcggagggca gattccggtt
     3661 ccggcaggag gatgtgagac aggtgcagat gggcaaggcg gcgatccgcg cagggatcga
     3721 gacgctgctc gcggaatacg gcggaggaga ccccgaagaa atcctgatcg caggaggttt
     3781 tggctgcagg atggacgagg agcagactat ccggatcgga ctgttcccgg aacaattccg
     3841 gggacatatc acgatgattg gcaacagtgc gcttgcggga gcacagcggt atctgctcga
     3901 cgacaggacg gcggcaaaac agaggcttgc gtctatcaca gggcacgcac aggaactttc
     3961 gcttgccatg cacccgaaat tcaacgaatt atatttggac gagatgtttt ttgatacaga
     4021 cagatgatgg gaggaaccgt tgatatggaa caaaaaaatg attttctcgg gagagatccg
     4081 attgggagac tgctgtggcg catgtcgctt ccagcgatcg ttgcacagct gatcaacctg
     4141 ttatataacc tggtagaccg ggtctatatc ggacatatgc cggaagacgg agcgctggcg
     4201 cttacagggg tgggcgtctg cctgcccatt atcatgattg tgtcggcatt tgccgcactt
     4261 gtgagcaacg gcggggcacc gagggcttcc atggcgatgg gaaagggcga ctacgacgag
     4321 gcagaaaaaa ttctcggcaa cagcgttgcg ctgcagattg tcatctccgc cgtgctcacg
     4381 gttctgctgt tactgtttta cagaccgctg ttgctgacgt tcggagcaag tgagaatact
     4441 atcggctttg cttcggatta tatgaaaatc tatgcggccg gaacggtctt tgtgcagctg
     4501 acgctcggca tgaatgcgtt tatcacggcg cagggctttg ccaggacagg aatgctcagc
     4561 gttatgatcg gcgcgggctg caatattgtg ctcgacccgg ttctgatcta tggattcggt
     4621 atgggagtcc gcggagcggc gaccgcgacg gtgatctcgc aggcggtatc gaccgtctgg
     4681 gttctctcat tcctgacggg aaaaaagact gtattaaaaa tccgcaaaaa gtatctgcgc
     4741 atggaagcgg gcgtggtact tccggggctg gcgcttggac ttgccccgtt tatcatgcag
     4801 gcaagcgaga gcgtgattac ggtctgcttc aatgcctcgc tgctcaaata cgggggagac
     4861 gtggcggtgg gagccatgac gattctatcc agtgcgatgc agttttccat gcttccgatg
     4921 cagggactcg gacagggcgc ccagccgatc acaagctaca attacggcgc cagaaatgca
     4981 gagcgcgtta aaaggacatt ccgtctgctg ttgaccacaa gcctgtgcta ttcgatggcg
     5041 gtatggcttt taatcatgct gtttccgcag atgtttgcaa tgattttttc ctccaacgca
     5101 gagctgatcg catacacgaa gtgggcgctc cgcatctact gtgccgccat gggtattttc
     5161 ggggcgcaga ttgcatgcca gatgacattt atctcgattg gcaatgcggg ggcttcgatc
     5221 attgtggcga ttatgcgtaa atttgtgctg ttgctgccgc ttatttatct gctgccgcat
     5281 ctggtgaaaa atcagaccat gggcgtgtat ctggcagagc cggcagcgga tgtgatcgcg
     5341 gtaagcttta cgcttgttct gtttttcttc cagttcaaaa aggcgattgc tgtcatagag
     5401 aagccgcaat agcgggtagg gcttttttcc tacttctttt gcccgaagta ttgaaatgcg
     5461 gcggtaactt tgctatgatg aagtgaggtt atgatgcgtt tgatgacgaa tgcagattag
     5521 gaatatagga cgaatcggat aggaaccagg agaataaaag aaaccggggt aagagtatgg
     5581 gcagcagaga taaggtactc atagtggacg attcagagct ggatcggatc gcattgaaaa
     5641 agatgctaaa ggatgcttat gagattgtag aggcagtaga cggtgcaggt gcacttcgga
     5701 ttctggaatc gcagaaggag tttgacgcga tatctgcgat cattctggat ctggtcatgc
     5761 cgcagatgga tgggtttgaa tttttaagag aataccgcaa ggtggattct tatcgtcgga
     5821 taccggtcat tgtggcgtct gtggagcgtg atgtcaagac ggaaaaggca tgtctggcac
     5881 tgggggcaag agactttata ggaaaaccgt atgatccgca gatcgtgcgg ttccgggtgg
     5941 aaaatgcggt gcgcagcagt gaacagcagc tttcgagaga attgagatac cgtgcggatt
     6001 acgacatgct caccggaatt tataataaga cgcatttctt tgaagcaacc cgtgttctgc
     6061 ttaagagaca tcccgaagag acgtatgcgt ttttacgtct cgacatcgaa aagttccaac
     6121 tggtgaattc tttcttcgga agcagcagcg gtgacggact tttacagtat atcgcgaagg
     6181 agatccggaa atttgcggga gatacggaac agattgccta cggaagaatc gaggcggata
     6241 ttttcggcat ctgcatgccg tacaggggcg aggagacgat gttagagctt gtgcgctaca
     6301 tgcgcatgcg tctcggacag taccagctcg cttttgatgt ggtgccggca atcggaatct
     6361 atgtgatccg ggagcgggat ctctccgttg atgcgatgta tgaccgggcg aatctggctg
     6421 ccaagcagtg caaaggcaat tacatcgaaa actatgcgtt ttatgtggat gagatgcggg
     6481 aagacatcgt gcgggaacag cagattgtca accatatgcg gcatgcgttg gacgaggagg
     6541 aattcgtcct ttacctgcag ccgaagtaca gcctgcagga caacaggatc tgtggggcgg
     6601 aagtgctggt gcggtgggca aagccggaag gcggcatgat ctcgccggga gaatttattc
     6661 cgatctttga gcgcaatgga ttcattacca agctggatta ctacgtctgg gagaagacct
     6721 gtaagctgct tgcaggatgg attgccgagg ggaaaaagcc gtcgccggtg tcggtcaaca
     6781 tttccagggt cagcctctat aatccaagac tggtggaggc aatctgcgga ctgacggacc
     6841 gctaccacat tccccgggaa ctgttggaac tggaactgac ggagagcgcc tacacgacga
     6901 acccgaaggc gatcaaggag acgatggaac gtctgcagaa ggaaggattt tctattctga
     6961 tggacgattt cggaagcgga tattcttctt taaatgtgtt aaaggatatt gccgtggatg
     7021 tgctcaagat cgatatgaag tttctgtcgg atacggatca ggaggggcgc agccagaata
     7081 ttcttgcgtc ggttgtgcgc atggcaaaat ggctgaatct cccggtgatt gcagagggcg
     7141 tagagagaag ggagcaggtg gactttctgc acagcgtcgg atgtgagtat gtacagggat
     7201 tttattttgc gagaccgatg ccggtgtccg attatgaaaa gctggtatcc gagaaaaagg
     7261 acgaactgca ggagaaatat cagaaggacg agatcggcag ggatatgctc tggaacgcga
     7321 cttcacagat gggaagcgtg ttctccggca tgatggagcc ggcggtcatc tatgaatatg
     7381 agaaagagca gaaccggatt gatgcgctgc gcgtgaacaa cgcctactat gacatgttcg
     7441 gatacggaga tatcaattcc tcgggcgggc ttgtgatgga aaaacaactc tcggacgaga
     7501 gcaggaagat tctcctcggc acgtttgagg atattgcaaa gtcgaaggag accgcagcct
     7561 gcgaattttt gcgcacttca aagagcggac gctcgatctg ggtacatctg aagttgaaat
     7621 acgtcacgag cgtgggcgaa aagcacatta
//
LOCUS       DBMDQJ010000014         7566 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4144_length_7566_cov_1.601608, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000014 DBMDQJ010000000
VERSION     DBMDQJ010000014.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4144_length_7566_cov_1.601608"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            337..6669
                     /locus_tag="ACZO9R_00595"
     CDS             337..6669
                     /locus_tag="ACZO9R_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HIR0911602.1"
                     /translation="MVFSTVAGDASFVTAYAAEVQTAAGSEAAENPSPAEESGADSTE
                     GAQNTINQESAAQTGNAGQTDAAQTEDAVQTDAAQTDGAAQADAVQNTESGADQNTAL
                     IQTAGAESLAAFSYTAAPEAGLPAAATAGSGTLDVSGVSYSGFSKGIAAKGWSEGTGF
                     TVAVNAAGYTDLKFSADMRSSGTGPANFALEYSIDGASWTKISGTDVTLSTGLASVYQ
                     QTALPAALSGKAFTLRVTMQGNTSVNQSTVGTNGVTSINNIVVTGTQGEAATDGTVCS
                     KVKASVEPGDVEAGTKVELSCATEGATITYHTDLDSADKTYTEALVIEEDVTIYATAE
                     LEGYETSETAEFAYAVKQGAKSKEAALATTLSDGNMVALYYENNGTGSVLSQTASGKK
                     LKGVSATAADGALAIPEDAAELTVSVDENGYYTFTCGGKYLTSGATGSSVTFADAAGD
                     YSLWKLQKANENGGYYVINANAKYNNTTEQYLEYYSGFTTYSYKSSSDAAYVFYFYLI
                     DEGTPSVHIRADESVTENVAQWSGTADYKTAGVTADTGIAGDSLNANDLLDADAIYSA
                     VVNGTAVAPYTEATSTSTGSTSYYMGGKGIGSGSNDYAQFVMPTTGYANMVLAFRLRA
                     SNTGAGSFQMQYSTDGVDFKNLSTGTYAYKYTKYVGGEPQEVSAAEKITDGVAKTSLA
                     PANYISFTFDIPNEAANADRLYIRMVPGSLSAKGDKAPAAGGVIRMDTVTVTGNPLIA
                     SDLCGYVAAEPQSGVVAVGSELTLTSATEGVTIHYSVNGGAEQIYDASAKPKLADLPA
                     VVTAYATKDGYKDSIRRTFSYQQAQVATVKATPNGGSVVKNTAVNLTCETEGATIQYS
                     ADDGATWQDYTEKLVLTELPVTYKVKAVKDGYLDSSVLTLSFTERTNEKYQIYFGQLH
                     SHTSYSDGAGSCEDAYQHATNVDNLDFVAVTDHSNSFDNADSASISDGSMSEEWKEGH
                     ALATQYTTSGFVCIYGFEMTWSNGLGHINTFNTEGFQSRTQNEYKTYSTALQNYYATL
                     KTQPDSISQFNHPGTTFGDFSDFAYYDEEIDKLITTIEVGNGEGAIGSSGYFPSYEYY
                     QRALDKGWHLAPTNNQDNHKGLWGDANTARSVVLVDSLTQENIYDAMRNYRVYATEDN
                     DLSIYYTLDGYIMGSILEDGATGDTVHLSVDLSDPTDASIGKVQVITNGGLVLAEKNV
                     SGNEETVTFDVKNDYSYYYIKVVEADSDIAVTAPVWVGSVEAAGIDSFSTDEVLPVRG
                     EPLTVNLGLYNNENSELAIDSITFEVGGETVHEADLAAAGLGSVASMSTGSYSFDYTY
                     DGVGSMEMTAVVHATMNGVQKVYKSVLKLSYATPEMVTKVIIDGTHYNDYVTGYYGGN
                     VGNFTAIAGAKNVKVEVVTDEITPEMLANCALLVISAPAKKSGTANAGDYTVSHFEDS
                     FIQMVKDYTDNGGTLITCGIADYQDSTSGQTATEMNKLLAAIGASTRLNSDEAYDETN
                     NGGQPYRLYLKGTYNKDSRYLRGASEEQEYSAYSGCTVALDADAVTAGRAEALVSGYD
                     TTYSIDCKDDAGNRVTGSPTYVEKGNMVALAHETLGSGANVFVAGTVFISDFEVKAEL
                     DNIWDLPYLNRTIAENILDEVKVELPLSTIAEVRKNGTAGDVYRVEGYVTAGTAVEGN
                     TFFDTIYIQDDTAGIDIFPYAESGLAIGTKVEITGYVDAYQGDKELKVISSRILDEEP
                     KVIAPEKLSAKDAMDYEKSGGKLVQVEGTVTDVLYDAAGTGVSQFWLDDGSGVNANIF
                     IDGYILSATTGKNELASVVSTGKRVSAVGVVYAHPEGTSDEAVTCLRVRNCDEIVEVT
                     SGEMPDDTETPGNTETPDTETPGDTGSSDSGTDSADGNGNGGSVSGSVTVSDGQTTDA
                     VTVASQNDWDPVRNRVTAASAGSTVTVLLADGVSVPGDVIAAVRGRDVRLVFRLANGI
                     VWTIDGRNVTSDTVPDMDFGVTINSHAVPDALARQTAAGRDYFQISLNHDGAFGFTAV
                     MTLPVGTQYAGMYAKLYYYNEAKGRLELASSGKVDANGNVELTFVHASDYVVVLDQMP
                     AVQTGDDTPLAVAVMMLLAGMVLLAAAARKKRIFLK"
     gene            6683..>7566
                     /locus_tag="ACZO9R_00600"
     CDS             6683..>7566
                     /locus_tag="ACZO9R_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR0911603.1"
                     /translation="MNFKKSKIRSLVLLALAAVAFCGLIWFANKDRPVYRSSNMAGTD
                     YEVGRVLRVVEDHKTVDEEMDGIWRGSMTLEVEILTGRYKGDTAIVENYFSSLYNVRV
                     AEGDKISIRIDTTGEDEYQVSVYNYYRVPQMIGCIVAFVLLLVLIGGKKGAKSVAGLA
                     FTVVCILWILLPLALKGYSPLAVTILLILVCNFVSFFLIDGISTKTVVAAIGSMLGVL
                     AGAVFALIAQNAMSITTYQMDEAETLLLITSTTELKIRDLFLCGILISCMGAVMDVAM
                     SICSAVAEIHQVDRSLGMA"
BASE COUNT         1992 a         1690 c         2312 g         1572 t
ORIGIN      
        1 atgcagggtg catcagccct tttttatttc ataggaaata ccgtgataca ttcatgtgtg
       61 aaagtagaac cttcctatga aataaaaaac gctccgcgag gatgcgcggc tcgcggtagg
      121 gaaaatatcg ctttacgaac cgctcgcgcg aggagaatct cgcaagcgag attctttttt
      181 acactttttt tacaaggctt taccggacaa gggtagaaga gaagattcat aaacgttaca
      241 gtttatttta catacaaaaa aatgagagga acagttgtga aaatgaagaa aaagaatcat
      301 tggaaaaaca tatgtgcgct ttccttcgca ttctgcatgg tgttttcgac ggtagcaggg
      361 gatgcgtctt ttgttacggc gtatgcagca gaggtgcaga cagcggccgg aagtgaagcg
      421 gcggaaaatc cgtcaccggc agaggagagc ggagcagaca gcacggaagg tgcacagaac
      481 acaataaatc aagaaagtgc ggcgcagaca gggaacgccg gtcagacgga cgcagcgcag
      541 acagaggatg cagtgcagac ggatgcagcg cagacagatg gtgcagcgca ggcggatgcg
      601 gtacagaata cggagtctgg ggcagaccag aatacggcgc tcatccagac ggcgggtgca
      661 gaaagccttg cggccttcag ttatacggcg gcaccggaag cgggacttcc ggcagcagcg
      721 acagcgggca gcggaacgct tgacgtaagc ggagtatctt acagcggatt cagcaaggga
      781 atcgcagcaa aaggatggag cgagggaacg ggctttacgg ttgcggtcaa tgctgcaggt
      841 tacacagatt tgaaattttc cgcagacatg cgctccagcg gaaccggtcc tgccaacttt
      901 gcactggaat attccattga cggagcgagc tggacaaaga tcagcggaac ggatgtgacc
      961 ttaagtacgg gtctagcctc ggtgtatcag cagacggcac tcccggcggc gctgtccgga
     1021 aaagcgttta cactgcgtgt gacgatgcag ggaaacacga gtgtgaacca gagtacagtc
     1081 ggcacgaacg gtgtgaccag catcaacaac attgtagtca ccggaacaca gggcgaggcg
     1141 gcgacagacg gaacggtctg ttccaaagtc aaagcttccg tggagccggg agatgtggaa
     1201 gccggtacga aggtggagct ctcctgtgcg acggagggag caacgatcac ataccatacc
     1261 gatctggaca gtgcagacaa gacatacacc gaagcgcttg tgatcgagga agatgtgacg
     1321 atctacgcga cagccgaact cgaaggttat gagacgagtg agacggcaga atttgcctac
     1381 gcggtaaaac agggtgcaaa gagcaaggag gcggcacttg ccaccacact ttcggacgga
     1441 aatatggttg ccctctatta cgagaacaac ggaaccggaa gcgtgctctc ccagacagca
     1501 agcggcaaga agttaaaggg cgtgtcagcg actgcggcgg acggtgcgct tgccattccg
     1561 gaagatgcgg cagaacttac ggtttccgtg gatgaaaacg gctattatac ttttacatgt
     1621 ggtggaaaat atctgacgtc cggcgcaacg ggcagctccg tgacgtttgc ggacgcggcg
     1681 ggagattaca gtctctggaa gctgcagaag gcaaatgaga acggcggata ctatgtgatc
     1741 aatgccaacg caaaatataa taataccaca gaacagtatc tggaatatta cagcggtttt
     1801 acgacataca gctacaaatc atcttcggat gcggcgtatg tattctattt ttatctgatc
     1861 gatgagggaa caccgtcggt tcatatccgt gcggacgagt ccgtcacgga aaacgtggca
     1921 cagtggtccg gaacggcaga ttacaagacg gcaggtgtga cggcagatac cggaattgca
     1981 ggagattctc tcaatgcaaa tgatctgctg gatgcggatg ctatatattc ggcggtggta
     2041 aatggaacgg cagtggcacc gtataccgag gccacaagca cttccaccgg ttcgacttcc
     2101 tattatatgg gaggaaaggg cattggaagc ggaagcaatg attatgcgca gtttgtgatg
     2161 ccgacaacgg gctatgccaa catggtgctc gcattccggc ttcgcgcaag caacaccgga
     2221 gcaggttcct tccagatgca gtattctacg gacggtgtgg attttaaaaa cctttccaca
     2281 ggaacctatg cttataagta cacgaaatac gtgggcggtg agccgcagga agtgagcgcc
     2341 gcggagaaaa ttaccgacgg tgtggcaaag acaagccttg cgccggcaaa ctatatttcg
     2401 ttcacgtttg atattccgaa cgaggcggcg aatgcagacc gtctctacat ccgcatggtc
     2461 ccgggcagcc tttctgccaa gggagataag gcaccggcag cgggcggagt gatccgtatg
     2521 gacacggtga cagtgacagg caatccgctg atcgcctcag atctctgcgg atatgtggca
     2581 gcagaaccgc agagcggcgt ggtagctgtg ggcagtgaac tgacactgac aagcgccacg
     2641 gagggtgtaa caatccatta cagtgtaaac ggcggcgcgg agcagatcta tgatgcgtcg
     2701 gcgaagccaa agcttgcaga ccttccggca gttgtgactg cctatgcgac aaaggacggc
     2761 tataaggaca gcatccgccg tacattctct tatcagcagg cacaggtggc aacggtaaaa
     2821 gcaacgccga acggcggcag cgtggtgaaa aataccgcgg tgaacctcac ctgtgagacc
     2881 gagggtgcga cgattcagta ttccgcggac gacggtgcaa cctggcagga ttataccgaa
     2941 aagctcgtcc tgacagagct tccggttacc tataaggtga aagccgtcaa agacggttac
     3001 ctggacagca gtgtgctcac actttccttt accgagcgca caaacgaaaa atatcagatt
     3061 tactttggac agttacattc ccacacttcc tattcggacg gtgcgggaag ctgtgaggat
     3121 gcataccagc atgcaacgaa cgttgacaat ctggattttg ttgcggtgac agatcactcc
     3181 aactcgtttg acaatgcgga cagcgcgtcc atctcggacg gctcgatgag tgaggaatgg
     3241 aaggaaggcc atgcgcttgc cacgcagtat acgacaagcg gatttgtgtg catctatggg
     3301 tttgagatga cctggtcgaa cggacttgga cacatcaata cgtttaacac cgaaggcttc
     3361 cagagccgta cccagaatga gtacaagact tacagcacgg cattgcagaa ttattacgcg
     3421 accttaaaga cacagccgga ttccatcagc cagtttaacc acccgggaac gacgttcggt
     3481 gatttcagcg atttcgcata ctatgacgag gagatcgaca agctgattac gacgatcgag
     3541 gtcggaaacg gcgagggtgc gatcggaagt tcgggttact tcccgtccta cgagtattac
     3601 cagcgcgcgc tggacaaggg ctggcatctt gctccgacca acaaccagga taaccataag
     3661 ggcttgtggg gcgatgcaaa cacggcgcgt tccgttgtcc ttgtggacag cctgacgcag
     3721 gaaaatattt acgatgccat gcgcaattac cgcgtgtatg cgacagagga taacgacctc
     3781 agcatctatt acacgctgga cggctacatc atgggttcca ttttagagga tggagcgacc
     3841 ggagataccg tacatctttc cgttgactta agtgatccga cggatgcgtc catcggtaaa
     3901 gtacaggtca tcacaaacgg aggactggtg cttgccgaga aaaatgtttc cggaaacgag
     3961 gagacggtca cctttgatgt gaaaaatgat tattcctatt actatattaa ggttgtggaa
     4021 gcggacagtg atatcgccgt gacggcaccg gtatgggtag gctctgtgga ggcggcagga
     4081 attgacagct tttccacgga cgaggtgctt ccggtaaggg gagagccgct gacggtgaac
     4141 cttggactct ataataatga aaacagcgag ctcgcgattg attccataac gtttgaggtg
     4201 ggcggagaga ccgtacatga ggcggatctt gccgcagcag gattgggaag cgtcgcatcc
     4261 atgagcacgg gaagctacag ctttgactat acctatgacg gcgtcggcag catggagatg
     4321 acagcagttg tgcatgcgac gatgaacggc gtgcagaagg tatacaagag cgtcttaaaa
     4381 ttaagctacg cgacaccgga gatggtgaca aaggttatca tcgacggaac ccattacaac
     4441 gattatgtga ccggttacta tggcggtaac gtcggaaact ttacggcgat tgcgggagca
     4501 aagaacgtca aggtggaagt ggtcacagac gagattacgc cggagatgct tgcaaactgt
     4561 gcgttgcttg tgatctcggc accggcgaag aaatccggga cagcaaatgc cggagattac
     4621 accgtgtcgc attttgaaga cagctttatc cagatggtaa aagactatac cgacaatggc
     4681 ggtacgctga ttacctgcgg tattgccgat tatcaggatt ctacttcggg acagacagcg
     4741 acagagatga ataagctttt ggctgcgatc ggggcatcga ccagattaaa cagcgacgag
     4801 gcgtacgatg agacgaacaa cggcggacag ccgtaccgct tatatctgaa aggaacctac
     4861 aacaaggatt ccagatatct gcgcggcgca agcgaggagc aggagtacag tgcgtacagc
     4921 ggatgtaccg tggcgctgga tgcggatgcg gtgacagccg gacgtgcgga agcgcttgtg
     4981 agcggatatg atacaacgta ttcgatcgac tgcaaggatg atgcgggaaa cagagttacc
     5041 ggaagcccga cgtatgtgga aaaaggcaat atggtagcac tcgcacatga gacactggga
     5101 agcggtgcaa atgtctttgt tgcgggaacc gtatttattt ccgactttga ggtgaaggcg
     5161 gagctggaca atatctggga tctgccatac ttaaaccgca cgattgcaga aaatatttta
     5221 gatgaagtga aagtggagct tccgctgtct acgattgcgg aagtgcgtaa gaacggtacg
     5281 gcgggcgatg tctaccgcgt ggaaggctat gtgaccgcag gaacagccgt ggagggcaac
     5341 accttctttg atacgatcta tatccaggac gacaccgcgg gaattgatat tttcccgtat
     5401 gcggagagcg gacttgccat cggaaccaag gtggagatta ccggatatgt ggacgcctac
     5461 cagggcgata aagagcttaa ggtgataagc agccggattc tggacgagga gccgaaggtg
     5521 attgcaccgg agaaactgtc ggcaaaagac gccatggact atgaaaagtc cggcggtaag
     5581 ctggtacagg tggagggaac ggtaacggat gtactctatg atgcggcggg caccggcgta
     5641 tcacagttct ggctggatga cggcagcggc gttaatgcca atattttcat cgacggatat
     5701 atcttatcgg caacgacagg aaagaatgaa ctcgcatcgg ttgtgtcgac aggaaaacgc
     5761 gtgtcggcgg tcggcgtggt ctatgcacat ccggagggaa cctcggacga ggcagtgacc
     5821 tgtctgcgtg taagaaactg cgatgagatc gtggaagtga cgtccgggga gatgccggat
     5881 gacacggaga cgccgggcaa taccgagaca ccggatacgg agacaccggg agatacaggt
     5941 tcgtcagaca gcggaactga cagcgcggac ggaaatggaa atggaggaag tgtaagcggc
     6001 agcgtgactg tatcggacgg acagacgaca gatgcggtga cggttgcttc gcagaatgac
     6061 tgggatccgg tcagaaacag agtgacagcg gcatccgccg gcagcacagt cacggttctc
     6121 cttgcagacg gtgtttctgt gccgggagat gtcattgcgg cagtgcgcgg aagggatgta
     6181 cgtctggtat ttaggctggc aaatggaatc gtgtggacga ttgacggaag aaacgtgaca
     6241 tcggatacgg ttccggatat ggactttggc gtgacgatca acagccatgc cgtgccggat
     6301 gcgcttgcga gacagaccgc ggcgggaaga gattatttcc agatcagctt aaaccatgac
     6361 ggcgcattcg gttttaccgc agtgatgacg ctgccggtgg gaacacagta tgcgggcatg
     6421 tatgcaaagt tatattatta caatgaagca aagggacggc tggaactggc atcttccgga
     6481 aaagtagatg caaacggaaa tgtggagctt acctttgtac atgcatccga ttatgtggtg
     6541 gtgctggatc agatgcctgc agtgcagacg ggggatgaca ctccgcttgc agtagcagtg
     6601 atgatgcttc ttgcaggtat ggttctgctt gcagcggcag cacgcaaaaa aaggatattt
     6661 ttgaaataag ggtggttaaa acatgaattt caaaaagagc aagatacgca gcctggtgct
     6721 tctggcactg gctgccgttg ctttttgcgg actgatctgg ttcgcgaata aagacagacc
     6781 ggtttatagg agcagcaaca tggcggggac ggactatgag gtcggaagag tcctgcgggt
     6841 tgtggaggat cacaagacgg tggatgagga gatggacgga atctggcggg gaagtatgac
     6901 gctggaggta gagatcctta ccggacgtta caagggcgac acggctattg tggaaaatta
     6961 tttcagctcc ctgtacaacg tcagggtggc ggagggggat aaaatctcca tccgcatcga
     7021 cacgacgggg gaggacgaat atcaggtctc ggtctacaat tattaccgcg ttccgcagat
     7081 gattggctgc atcgtggcgt ttgtgctgct tctcgtattg atcggaggga aaaaaggtgc
     7141 aaaatctgtg gcggggctgg cgtttaccgt ggtatgtatc ctctggattt tactgccgct
     7201 tgcgctgaag ggttactcgc cgctggcggt cactatcctg ctcattctgg tctgcaattt
     7261 tgtcagtttc ttcctgatag acggcatctc gaccaagacg gttgtggcgg caatcggaag
     7321 catgctgggt gtgcttgcgg gagcagtgtt tgcactgatc gcgcagaatg ccatgtcgat
     7381 tacgacttac cagatggatg aggcggagac actgcttctg atcacaagca ccacggaact
     7441 gaagatccgg gatctgtttt tgtgcggtat tctgatttcg tgtatgggag ccgtgatgga
     7501 tgtggcgatg agtatctgtt ccgcggtggc ggagatccat caggtggatc ggtcgcttgg
     7561 catggc
//
LOCUS       DBMDQJ010000015         7557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4152_length_7557_cov_1.741014, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000015 DBMDQJ010000000
VERSION     DBMDQJ010000015.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7557
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4152_length_7557_cov_1.741014"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="ACZO9R_00605"
     CDS             complement(<1..589)
                     /locus_tag="ACZO9R_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR0911604.1"
                     /translation="MKRKKEVALLLLTAVLAAAGCADEKTTGEKTTESGSAAAVSGSS
                     VFDGELEENVTIRVLENDTAISKGYFEELITAFNEAYADQGITAVDANMDQYLDLAND
                     GPYGYGPDVLYQANDVIMQYAKGKHIYPLPVQQLSCYEQIPESAWKAYETTVDSTTYC
                     CGVPVNVQAPMLYYRADLLPEDWQENWDEDQNGVPD"
     gene            complement(624..>2352)
                     /locus_tag="ACZO9R_00610"
     CDS             complement(624..>2352)
                     /locus_tag="ACZO9R_00610"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HIR0911605.1"
                     /translation="QYCFAVGEKKLVIRLRMAKEDADASVTLIYAGKYQFQQSREQIP
                     MEIRYTDRLYHYYETTLVLTDVRLAYVFCIRQQGTTYYFCEDGVVLQYNFAEGFYNFF
                     QMPYINRNDVLPTVKWMKSAVFYQIFVDRFFCGNTKKDRSYINMEWGALPTPKSFAGG
                     DLCGIIEKLDYLKDLGITAIYLTPIFRSISNHKYDTMDYMQIDPQFGTKEEFGELVRM
                     AHAHGIRIVLDAVFNHCSMQMQQFCDVMEKGAESPYYDWFLIDGAYPVPSRANYECFA
                     ECTYMPKLDTANREVQEYLIGIACYWIREYGIDGWRLDVSDEVSHVFWRRFREAVKKE
                     KADAVIIGENWHDAYPYLRGDQYDSIMNYAFTKACLDYFARKTFDAKQMAEKLSSNLM
                     RNMEPVNRMMLNLLDSHDTHRFYSEVGENPDRLLAALALEMVFPGAPCIYYGTEICTP
                     GGYDPDSRRCFDWEKVHTDTDVRKKLTELTHIRSCTAITDGNVELAAENGMLCVRRKA
                     GGEGVSLYINMTEEQRRQEHCLKADSKVLSAHRASVLPAAGDGKMTCGVCVEPEGYLI
                     VREQREALD"
     assembly_gap    2353..2362
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2527..3327
                     /locus_tag="ACZO9R_00615"
     CDS             2527..3327
                     /locus_tag="ACZO9R_00615"
                     /inference="COORDINATES: protein motif:HMM:NF012576.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0911606.1"
                     /translation="MSQKITIRDVAREAGVSVATVSYVINNRTDMRISDATRKKVLQV
                     INLLDYTPNQSAKALATSRNRMVALYLDTDVPALKRAEQMYLLECFSTFLHAQDYDLI
                     YLSRTYTEKYDAADAIVAYDISEEYFHQIGDNNFSPLLALDCRIGDPLFFEIYSDYAQ
                     MATGAHTFFSGEPYCFLSLDTSNAGKKSYAADMFPEVHYVNSPSELAPFSGRNLLVTD
                     HTLYELLASTANVCYLPSFSARKFQVLFDCMNSALRREPLQQHAIAVS"
     gene            complement(3335..4504)
                     /locus_tag="ACZO9R_00620"
     CDS             complement(3335..4504)
                     /locus_tag="ACZO9R_00620"
                     /inference="COORDINATES: protein motif:HMM:NF021028.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HIR0911607.1"
                     /translation="MRRRNDGAAVAMTAMLVSGMLLLSACGSADAVKTDATGATEEMA
                     QTGQAAACVVTYYDSDGKTVLQTEEVEKGACAKEYTPEKDGSIFVGWFATPQMSHKFD
                     FSQAVTEDTSVFAGFVTYVEDTREFAIVGSGTSPVLLESNWGAVIGDAQKMTKEESDS
                     ENVYTITLDLSEGDQFQFAMDSSWGDQRGYGYLDTIELDGMDYFENSGSLGDTGVKRS
                     NIKCAVAGNYTFTLTTYPGEDTYETDNANYTEENREAFNINPYDTITWTYNGASENAG
                     GDVQTDYYIKGAIVTGWEDVYSDETKFTEQDGTYTLKIDLTEGDEFMFTSMVTVGDTS
                     SAGTEYIRYTNIGGGDDTSLSHVTEGGGENLIAAESGTYVFTYDPAEQVLTVDVE"
     gene            complement(4765..>5490)
                     /locus_tag="ACZO9R_00625"
     CDS             complement(4765..>5490)
                     /locus_tag="ACZO9R_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HIR0911608.1"
                     /translation="EVVDLVQPHDVLGQKIGDKLPENEALRTMMKKSYDILVNHPINI
                     ERKKKGLNPANSCWFWGAGTKPALYPFTERTHKQGAMISAVDLLKGIAVGTSMKVITV
                     DGANGGLDTNYEGKANAALDVLTKDGYDFVYVHLEGPDEMGHQGSVEKKVKAIERLDE
                     RIIRPIAEGLAEAGEDFRMVVLPDHPTPISLRTHTADNVPYLLYDSTAERSENWHYNE
                     REAAQTANFIPEGHKLIEYLLQM"
     assembly_gap    5491..5500
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5581..6051)
                     /locus_tag="ACZO9R_00630"
     CDS             complement(5581..6051)
                     /locus_tag="ACZO9R_00630"
                     /inference="COORDINATES: protein motif:HMM:NF021646.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911609.1"
                     /translation="MKYLVILGDGMADRPIESLGGRTPLEYAKTPKMDELAAKGEIGM
                     VHTIPDGMKPGSDTANLSVLGYNPREFYSGRSPLEALSIGVPMKDTDVALRCNIVTLS
                     EEEDNYEDRTIIDHSSGEISTEECAVLLEAVKKELETDIFHFYVGTSYRHSTDR"
     gene            complement(6108..7313)
                     /locus_tag="ACZO9R_00635"
     CDS             complement(6108..7313)
                     /locus_tag="ACZO9R_00635"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR0911610.1"
                     /translation="MIRVAVMGYGTIGSGVVEVLDINKNSIAKRAGEEIGVKYILDLR
                     DFPGDPIQEKVVHDYKVIANDPEVQIVVETMGGVEPAYTFVKAMLEAGKHVTTSNKAL
                     VAAKGAELIALAKEKNVNFMFEASVGGGIPIIRPLNSCLTADEIEEITGIVNGTTNYM
                     LTKMTEEGSEFDDVLKDAQAKGYAEKDPTADIEGYDACRKIAILTSLVCGQQVDFEDI
                     HTEGITKITATDIRYAKAMGRAIKLLASSHKVGDGYVAMVAPFLLPATHPLYNVNDVF
                     NAIFVHGNVLGDAMFYGSGAGKLPTASAVVADIVDMAKHLDTNIAVEWSSKKLELVDY
                     RNVENCYFVRTSADEKRIEEVFGEVSYVTAEGVSGETGFVTARMSEADYEKKAAELGS
                     VLQMIRVAQ"
     gene            complement(7350..>7557)
                     /locus_tag="ACZO9R_00640"
     CDS             complement(7350..>7557)
                     /locus_tag="ACZO9R_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR0911611.1"
                     /translation="PGLLSIVLKTVAEFHANILTIHQSIPINGIASLTLSVDVLPETG
                     DVTQMVASIEEQTGIHYVKILGRE"
BASE COUNT         1593 a         2307 c         1616 g         2021 t
ORIGIN      
        1 tatccggcac gccgttctga tcctcatccc agttctcctg ccagtcctcc ggcagcagat
       61 ccgcccgata gtacagcatc ggcgcctgca cattgaccgg gacaccgcag cagtaggtcg
      121 tgctgtccac ggtcgtctca tatgccttcc acgcgctctc cggaatctgc tcatagcagg
      181 acagctgctg taccggaagc ggatagatat gttttccctt ggcgtactgc atgatgacat
      241 cgttcgcctg gtataacaca tccggtccat agccataagg tccgtcattt gccagatcca
      301 ggtactgatc catgttggca tccaccgccg tgattccctg atcggcgtac gcctcattga
      361 atgcggtaat cagctcctca aaatatccct tggaaatggc ggtgtcattt tccaataccc
      421 ggatcgtgac attctcctcc agttcaccgt caaaaacact gcttccggat accgcggcag
      481 cgctgccgct ctccgtcgtc ttctcccccg tcgtcttctc atccgcacat cctgctgccg
      541 caaggacggc tgtcagaagt aacagtgcca cctctttttt tcgtttcatc tgcttcctct
      601 tttctgcgga atttccgccc atatcaatcc aacgcttccc tctgctctct cacaatcaga
      661 tacccttccg gctccacgca gacgccgcag gtcattttcc catcaccagc tgccggaaga
      721 accgatgccc ggtgagcaga aagcaccttg ctgtccgctt tcaggcaatg ttcctgccgc
      781 ctctgctcct ccgtcatatt gatgtacaac gatacgccct cgcccccggc cttccgcctg
      841 acacagagca tcccgttttc cgctgcaagc tccacatttc cgtcggtgat cgcggtgcag
      901 ctgcggatat gcgtcagctc cgtcagcttc tttcgcacgt cggtatcggt gtgcaccttt
      961 tcccagtcaa agcatctgcg ggaatccgga tcatagccgc ccggcgtgca gatttccgtg
     1021 ccgtaataga tgcacggtgc tcccgggaat accatctcca gtgcaagcgc cgccagcagt
     1081 ctgtcgggat tctccccgac ttccgagtaa aagcggtgcg tgtcgtgcga atcgagaaga
     1141 tttaacatca tccggttcac cggctccatg ttgcgcatca ggttggacga cagcttttcc
     1201 gccatctgtt ttgcatcaaa cgtttttctc gcaaaataat ccaggcaggc tttggtaaat
     1261 gcataattca tgatactgtc gtactggtcg ccgcgcagat acggatatgc atcatgccag
     1321 ttctccccga taatgaccgc atctgctttt tcctttttga ccgcctcgcg gaagcgccgc
     1381 cagaacacat gcgacacctc atccgagacg tccagccgcc agccgtcgat tccgtattcc
     1441 cggatccagt agcacgcgat cccgatcaga tattcctgta cctcgcggtt tgccgtgtca
     1501 agctttggca tataggtaca ctccgcaaaa cattcataat tcgcccggct tggaaccgga
     1561 tatgccccgt cgatcaaaaa ccagtcgtag tagggtgatt ccgctccttt ttccatgaca
     1621 tcgcaaaact gctgcatctg catgctgcag tgattgaaca ccgcatccag cacaatccgg
     1681 attccgtggg catgcgccat gcgcacgagt tccccgaatt cttcttttgt cccgaactgc
     1741 ggatcaatct gcatataatc catcgtatca tacttgtgat tcgatatcga ccggaagatt
     1801 ggtgtcagat agatcgccgt aattccaagg tccttcagat agtccagttt ttcgataatt
     1861 ccacagagat ctccgcccgc aaaacttttc ggtgtcggca gcgctcccca ctccatattg
     1921 atataggagc ggtccttttt tgtatttcca cagaaaaaac ggtccacaaa aatctgatag
     1981 aacaccgcgc tcttcatcca ttttaccgtc ggcagtacgt cattgcggtt gatgtacggc
     2041 atctggaaga aattgtaaaa tccctccgcg aaattatatt gtaagacgac gccgtcctcg
     2101 cagaagtaat acgtcgtgcc ctgctgccgg atacaaaata cgtatgccag ccttacgtcc
     2161 gtgagcacga gggtcgtctc atagtaatga taaagccggt ccgtataacg tatctccatc
     2221 ggaatctgct ctctgctttg ctgaaactga tacttgcccg catagattaa cgttaccgac
     2281 gcgtccgcgt cttccttcgc catccggagt cttatcacca gttttttctc tccgactgca
     2341 aaacagtact ggnnnnnnnn nngtactggg aatccggtat gtgtaaaatt gcactctcat
     2401 tcattgctct ttcctcttta ttctctatcc cttcattctc ttcttcccga ttcccttatt
     2461 gacaaatccg ggcaaattct atataaatgg aacatagaat tgctcattac aggggaggac
     2521 attatcttga gtcagaaaat cacgatccgg gacgttgccc gcgaagccgg cgtatcggtt
     2581 gccaccgttt catacgtgat caacaaccgc accgacatgc gtatcagcga cgcaacccgc
     2641 aaaaaagttc ttcaggttat caacctttta gactacactc ccaaccagtc cgccaaggca
     2701 ctggcgacaa gccgcaaccg gatggtcgca ctctatcttg acactgatgt cccggctctt
     2761 aagcgcgcgg agcagatgta tctgctggaa tgtttctcca cctttctgca tgcgcaggat
     2821 tatgatctca tctatctcag ccgcacatac acggaaaaat acgatgctgc tgacgcgatc
     2881 gtggcctacg acatttccga agaatatttt catcagatcg gcgacaacaa tttcagtccg
     2941 cttcttgcct tggactgccg gatcggcgat cccctgtttt ttgaaatcta ttccgattat
     3001 gcacagatgg caaccggagc gcacacgttc ttttccggcg agccctattg ttttctctct
     3061 ctcgacacgt caaacgccgg gaagaaatct tacgccgcag acatgtttcc ggaagtacat
     3121 tatgtaaact ccccttcgga actggcaccg ttttccggcc ggaacctgct cgtgacggat
     3181 cacacgctgt acgagctgct ggcgtccacc gcaaatgtct gctacctgcc atctttctct
     3241 gcacgtaagt tccaggtatt atttgactgc atgaacagcg cgctgcgccg tgagccgctg
     3301 cagcagcacg ccattgcagt gtcataaagc tgttttattc cacgtccacc gtcaatacct
     3361 gctctgcggg atcatacgtg aacacatagg tgccgctctc cgctgcgatc agattctccc
     3421 cgccgccttc cgtcacatgg gaaaggctgg tgtcatcccc gccgccaatg tttgtgtagc
     3481 ggatatattc ggttcctgcg ctgctggtat ccccgacggt caccatggag gtaaacataa
     3541 attcatctcc ctccgtcaga tcaatcttta aagtgtatgt gccatcctgc tcggtaaact
     3601 tcgtctcatc cgaatacaca tcttcccatc cggtcacaat cgcacccttg atgtaataat
     3661 ctgtctgaac atctccgccg gcattctccg atgctccgtt gtatgtccat gtgatcgtat
     3721 cgtacggatt gatgttgaac gcttccctgt tctcctccgt atagtttgcg ttgtcggtct
     3781 cgtaggtatc ctctcccgga tacgtcgtca gtgtaaacgt ataatttccc gccaccgcac
     3841 acttgatatt ggagcgtttc acgccggtgt cgccaaggct tccggaattt tcaaaataat
     3901 ccatgccgtc cagttcaatc gtatcgagat acccgtagcc gcgctgatct ccccaggagg
     3961 aatccatggc aaactggaac tggtctccct cgcttaaatc gagcgtgatc gtatacacat
     4021 tctcgctgtc gctctcctcc ttcgtcatct tctgcgcatc cccgatgact gctccccagt
     4081 ttgattccag aagtaccggc gacgtgccgc ttcccacaat cgcaaactct ctggtatcct
     4141 ccacatacgt cacaaatccg gcaaacaccg aggtgtcttc tgttaccgcc tgtgaaaaat
     4201 caaatttgtg gctcatctgc ggtgtcgcaa accagccgac aaaaatgctt ccgtccttct
     4261 ctggcgtata ctctttggcg catgcccctt tttctacctc ctccgtctgc agcacggtct
     4321 tgccatccga gtcgtaatag gtcacaacgc aggctgccgc ctgcccggtc tgcgccatct
     4381 cttccgttgc cccggtcgca tccgtcttca ccgcatccgc acttccacat gcggataaca
     4441 gcagcatccc gcttaccagc attgcggtca ttgccaccgc cgccccatca tttcttcttc
     4501 tcatttttcc ctccaacctt tctatgagac ttctttgtag ttcttgttga ttaaccggtt
     4561 aagttttcgt gtatttttta agcaaaccgc tatattctgc agtttgctta accgtttaat
     4621 ctataacaaa tgtaacactc ttcctgcagg ctgtcaatag tattttgtgc atcccgcccg
     4681 gcacgttttt gtgtcataga aacgaagttt gctatgacac aaaaaaatcc accttcctac
     4741 cctaaatagg aagatggatt tttcttacat ctgcagcaga tattcgatca gtttatgtcc
     4801 ttccggaata aagttggctg tctgcgccgc ctcgcgctca ttgtaatgcc agttctcact
     4861 gcgctctgcc gtgctgtcat agagcagata cggtacattg tccgccgtgt gtgtgcgcag
     4921 ggaaatcggt gtcggatgat ccggaagcac caccatgcgg aaatcctctc ctgcctccgc
     4981 aagtccctcg gcgatcggcc ggataatgcg ctcatccagg cgctcgatcg cctttacctt
     5041 cttctcaacg ctgccctggt gtcccatctc atccggtcct tccagatgca cgtagacaaa
     5101 gtcatatccg tccttcgtca ggacatctaa cgccgcgttc gcttttccct cataatttgt
     5161 atccagaccg ccgttcgcac cgtccactgt gatgactttc atggaagtgc cgaccgcaat
     5221 tcccttcaga agatcaactg cagaaatcat cgcgccctgt ttgtgggtgc gctccgtaaa
     5281 cggatagagt gccggctttg tgcctgcgcc ccagaaccag caggagttcg caggatttaa
     5341 tccctttttc ttacgctcga tgttgatcgg atgattcacg agaatgtcat agcttttctt
     5401 catcatggtg cgaagcgctt cgttctccgg aagcttgtct ccgatcttct gtcccagcac
     5461 atcgtgcggc tgcaccagat ccaccacctc nnnnnnnnnn tatgaatacc aaccagagtg
     5521 acatccggat ggctggtttc ccagtaattt aaagtttcgt tgatcatgga agaaccattg
     5581 tcatcgatca gtgctgtggc ggtagcttgt gcccacataa aaatgaaaaa tgtctgtctc
     5641 cagttccttc ttcaccgcct ccaagagcac tgcacattcc tcggtactga tctcacccga
     5701 actgtggtcg atgatcgtgc gatcctcgta attgtcctcc tcctcggaaa gcgtgacgat
     5761 gttgcagcgc agagccacat ccgtatcttt cattggaaca ccgatagaga gtgcctcaag
     5821 cggagaacgc cccgaataaa attctctcgg attgtatccc agcacggaga gattggcagt
     5881 gtcgctgccc ggcttcatgc cgtccgggat cgtatgtacc atgccgatct cacctttcgc
     5941 tgccagttca tccatcttcg gtgtctttgc atactctaac ggagtcctgc cgcccagact
     6001 ctcgatcggg cggtctgcca tgccatctcc aagaattacc aaatacttca tctcacactt
     6061 cactttctgc tgttatcagc ctgctgtttc acatcggatc ccctgtttta ctgtgccacg
     6121 cggatcatct gaagtacact gccaagctcc gccgcctttt tctcataatc agcttcgctc
     6181 atgcgcgccg tcacaaatcc ggtctctccg gatacgcctt ccgccgttac ataactcact
     6241 tcaccgaaga cttcttctat gcgcttctca tctgcggacg tgcgcacaaa atagcagttc
     6301 tcgacgttgc ggtaatccac cagctccagt ttcttagagc tccactccac cgcgatattg
     6361 gtgtccagat gcttcgccat atccacgata tccgctacaa ccgcgctcgc cgtcgggagc
     6421 ttgccggcgc cgcttccgta aaacatggca tcgccgagca cattgccgtg tacaaagatg
     6481 gcattgaaca catcgttgac attgtagaga gggtgtgtcg ccggtaaaag gaacggcgct
     6541 accatcgcca catatccgtc tcccaccttg tggctggatg ccaaaagctt gatggctctg
     6601 cccattgcct tcgcataccg gatatcggtc gccgtgatct tcgtaatacc ctctgtgtga
     6661 atatcctcaa agtccacctg ctgtccgcat accagggatg tcaggatggc gatcttgcgg
     6721 caggcatcgt atccttcaat gtcagcggtc gggtctttct ccgcataacc cttcgcctgc
     6781 gcatccttta acacatcgtc gaactccgat ccctcctcgg tcattttcgt cagcatatag
     6841 ttcgtggttc cgttcacaat tccggtgatc tcctcgatct catctgccgt cagacaggaa
     6901 ttgagcggcc ggataatcgg gataccgccg ccgacgctcg cctcaaacat gaaattgaca
     6961 tttttctcct tggcgagtgc aatcagctcc gcaccctttg ccgcaacgag cgccttgttg
     7021 gaggtcgtca catgttttcc cgcttcaagc attgccttta cgaacgtgta cgccggctcc
     7081 acaccgccca tcgtctcaac aacaatctgc acttccgggt catttgcgat caccttataa
     7141 tcatgtacca ccttttcctg aatcggatcc cccggaaaat ctcttaagtc cagaatatat
     7201 ttcacaccaa tctcttctcc ggcacgcttt gcaatgctgt tcttattgat gtctaatact
     7261 tccactacgc cggaaccgat ggttccatat cccattactg ctactctgat catactgcct
     7321 ccactttcgt cttctatcct attctgttcc tactctcttc cgaggatctt cacataatgt
     7381 atccctgtct gctcctcgat gctcgccacc atctgtgtca catctcccgt ctccggcaga
     7441 acatcgaccg agagtgtcag cgatgcgatc ccgttgatcg gaatactctg gtggatcgtc
     7501 aggatattgg cgtgaaactc tgccaccgtc ttgagcacaa tcgacaacag ccccggc
//
LOCUS       DBMDQJ010000016         7538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4167_length_7538_cov_1.675676, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000016 DBMDQJ010000000
VERSION     DBMDQJ010000016.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7538
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4167_length_7538_cov_1.675676"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="ACZO9R_00645"
     CDS             <1..372
                     /locus_tag="ACZO9R_00645"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR0911612.1"
                     /translation="ENYKERIVKARDIDSEVTGMSTGHPIRVLSNAMSREYLKLEKEG
                     AGFEELEQLTLGSLRKAVMDGDVVHGSLMAGQCAGLVKKEQTCQEMIEELMAQTAGLL
                     GDGDFWEKKSVFLGRTSDKGE"
     gene            374..1309
                     /gene="fabD"
                     /locus_tag="ACZO9R_00650"
     CDS             374..1309
                     /gene="fabD"
                     /locus_tag="ACZO9R_00650"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR0911613.1"
                     /translation="MGKTAFLFPGQGAQYIGMGRDFYEQIPVSRDVFAAASEASGLDI
                     AALCFEENEKINITEYTQIGMLTVEAAILQALLAEGRTPDVAAGLSLGEYGALLASGV
                     MEQRDAFALVRKRGIYMQEAVPVGGAMAAVLGLDAEKIEKICGQTAGTVSIANYNCPG
                     QIVITGEEAAVAAAGEQCKGSGAKRVVPLKVSGPFHSPMLKEAGEKLGEALAAVTLRE
                     IKLPYLANVTADYVREAEAVKPLLKQQVCAPVRWQQSVERLLADGVDTFVEIGPGHTL
                     SGFMKKILKQAEVPADTVQVYNVETMDDFKEYMSK"
     gene            1323..2048
                     /gene="fabG"
                     /locus_tag="ACZO9R_00655"
     CDS             1323..2048
                     /gene="fabG"
                     /locus_tag="ACZO9R_00655"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR0911614.1"
                     /translation="MLEGKVALVTGAGRGIGAAIAETLAREGAFVVVDYRGSGQAAQE
                     LAEKIGGVAMQCDVADFEACENMIKTMIETYGHLDILVNNAGITRDGLLMKMSEADFE
                     AVLSTNLKGTFHTIRHASRYFLKQRYGRIVNISSVSGVMGNAGQANYSASKAGVIGLT
                     KSVARELASRGITCNAVAPGFIETDMTDAMTESAKEAVQKQIPLGRCGNTKDVAELVT
                     FLASDKAAYITGQVISVDGGMCM"
     gene            2081..3322
                     /gene="fabF"
                     /locus_tag="ACZO9R_00660"
     CDS             2081..3322
                     /gene="fabF"
                     /locus_tag="ACZO9R_00660"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR0911615.1"
                     /translation="MRRRVVVTGMGVITPIGNSVEEFWNAVREEKTGFGEITHFDASG
                     YKCHLAAEVKNFDGKNYMDPKAAKRMELFCQYAVAATKEAIADAGLEMEKEDPYLVGT
                     AIGSGVGSLQAIEREYKKLLEKGPGRVNPLLVPMMISNMAAGNVSIQFGFKGKSINDV
                     TACATGTNTIGEAFRSIQYGEADIMIAGGTEGSICPIGIAGFTALTALSSESDPKRCS
                     LPFDKERSGFVMGEGAGIVVLEELEHAKQRGAKIYAEVAGYGCSSDAYHITSPAEDGA
                     GAARAMENAMKDAGVKPDEIPYINAHGTATHLNDLFETRAIRLAFGEHATKLKINSTK
                     SMIGHLLGAAGAVEFVTCVKEMQENYVHATVGYQTPDEELDLDYCREAVAMEVPYALS
                     NSLGFGGHNASLLLKRYGGEA"
     gene            3322..3759
                     /gene="fabZ"
                     /locus_tag="ACZO9R_00665"
     CDS             3322..3759
                     /gene="fabZ"
                     /locus_tag="ACZO9R_00665"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HIR0911616.1"
                     /translation="MELEIGQIMEILPHRQPFLLIDAVEELEPGKRAVARKCVSYNEP
                     YFGGHFPGEPVMPGVLIVEALAQTGAVAILSMEENCGKTAYFAEINRARFKKKVVPGD
                     VLTLTTEIIRQKGPIGIGSATASVRGEVVCTAELTFAIGSKEE"
     gene            3767..5476
                     /locus_tag="ACZO9R_00670"
     CDS             3767..5476
                     /locus_tag="ACZO9R_00670"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HIR0911617.1"
                     /translation="MAALFQKRKKEPAEKTPVEAAQALQETIVCPACGREINKKEAEK
                     NLYVCYECGSYFRVRTRNRIRMVADVGTFTPWFEDLAEANPLEFPGYEDKVKAAKEKT
                     GLHEAVTIGHCKIYGEDAVLGICDARFLMSSMGHVMGEKIACAVERATEEGLPVILFC
                     CSGGARMQEGIVSLMQMAKTSAALKRHSEAGLLYIPVLTDPTTGGVTASFAMLGDIIL
                     AEPGALIGFAGPRVIEQTIGEKLPEGFQRAQFQLEHGFVDRIVERKELKLTLYRILKL
                     HHKKEGYANFDPVREADCLEATELMKERATKAKALSAWEKVKAARKVDRLASVDYIEQ
                     IFDEFMELHGDRQFRDDPAIVGGVAYLDGQPVTVIGVHKGKDLKECMHRNYGMPSPEG
                     YRKALRLMKQAEKFKRPVITFVNTSGAFCGMEAEERGQGEAIARNLYEMSGLKVPVLC
                     IMIGEGGSGGALALSVGNEVWMLENATYSILSPEGFASILWKDGRRAKEASGVMKITA
                     QDLKALGVIEEIIPEYGMADQPALISISRYMKRHIKTFLKKQNGKTGDQLAAERYQRF
                     RVF"
     gene            5494..6477
                     /locus_tag="ACZO9R_00675"
     CDS             5494..6477
                     /locus_tag="ACZO9R_00675"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HIR0911618.1"
                     /translation="MKVKIVGTGSAVPEKCVTNDDLSQWMDTSDEWIRSRTGIETRHL
                     AVRETTTEMAVEAAKRALASAHMDAEELDLIIAATITPDRLIPNLACEVQSALGACRA
                     VAFDVSAACSGFLYAMDIARHYLEAGGSRRALVIGAETLSRIMDWSDRSTCVLFGDGA
                     GAAVLEADEAEEHAGILSTVLGTDGAGGMVLRCDNRRNHNPYVKGDTALSYVSMNGQE
                     VYKFAVRTVPKVIAGAVEAAGLSMDEIDFFLLHQANLRIIEAVAKRLHQPMEKFPTNL
                     QKYGNISSASVPILLDNVNNHGMIGEGSKIVLAGFGAGLTWGAAVLRWQAV"
     gene            6524..6976
                     /locus_tag="ACZO9R_00680"
     CDS             6524..6976
                     /locus_tag="ACZO9R_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0911619.1"
                     /translation="MTTDETLNELLVRLFKDIMEIEARCLVNEEFKDISYNDFHIIEA
                     IGLKEPKSMTAVAKLMDVTTGTLTKAMDGLTEKGYAVRERSKQDKRVVWVYLTDKGKA
                     AYAHHEEFHRKMIANIKGQLNEQETPILIYTLAKLVDYFQEEYGLEEE"
     gene            6981..7244
                     /locus_tag="ACZO9R_00685"
     CDS             6981..7244
                     /locus_tag="ACZO9R_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR0911620.1"
                     /translation="MSRGALGKNNWALFLMLLAGIMLGGFIGNAAAGVSGLSWLNYGQ
                     SFGFASPIVLDLGILVITFGLSIKITIASIIGVLIAIIIYRLI"
BASE COUNT         1983 a         1536 c         2495 g         1524 t
ORIGIN      
        1 gaaaattaca aggaacggat cgtaaaggcg cgcgacattg attccgaggt tacgggcatg
       61 agcaccggac atccaatccg ggttctgagc aatgcgatga gccgcgaata tttaaaactc
      121 gaaaaagagg gcgccggatt tgaggaactg gagcagctga cgcttggctc cctccgaaag
      181 gcagtgatgg acggggatgt ggtacacggc agcctgatgg caggacagtg cgcgggactt
      241 gtaaaaaaag agcagacctg tcaggagatg atagaggaac tgatggcgca gaccgcgggg
      301 cttttaggtg acggagattt ttgggaaaag aagagcgtct tcttgggacg cacatcggat
      361 aagggagaat gacatgggaa agacagcatt tttgttcccg ggacagggag cacagtacat
      421 cggtatgggc agagattttt acgagcagat tcccgtgagc agggacgtgt ttgcggcggc
      481 atccgaggcg tccggtctgg atattgcggc gctctgcttt gaagaaaatg agaaaataaa
      541 tatcaccgaa tacacacaga ttggcatgct gaccgtggag gcggcgatcc ttcaggcgtt
      601 gcttgcagaa ggaagaacac cggatgtggc ggcaggatta agtctcggag agtacggggc
      661 actgctggca agcggcgtga tggagcagag ggatgcgttt gcgcttgtga gaaagcgcgg
      721 catctacatg caggaggcgg tgccggtcgg cggcgcgatg gcggcggtgt tgggactgga
      781 tgcggaaaaa atagagaaga tctgtggaca gactgctggc acggtatcca ttgcgaatta
      841 caactgcccg ggacagatcg tgatcaccgg ggaggaagcg gcagttgccg cggcaggtga
      901 acagtgcaaa gggagcggcg cgaagcgtgt ggtgccgctt aaagtcagcg gaccgtttca
      961 ctcgcccatg ctaaaagagg cgggagaaaa gctgggagag gcgcttgcgg cggtaacact
     1021 ccgggagata aagctgccgt atctggcgaa tgtgacggcg gactatgtcc gggaggcgga
     1081 ggctgtgaag ccactgctga aacagcaggt gtgtgcaccg gtacgctggc agcagtccgt
     1141 ggagcgtctg cttgcggatg gtgtggatac gtttgtggag atcggcccgg gtcatacact
     1201 gagcggattt atgaaaaaga tcttaaagca ggccgaagtc ccggcagata ctgtacaggt
     1261 atacaatgtg gaaaccatgg atgacttcaa ggaatatatg tcaaagtaga ggagagagtc
     1321 agatgctgga aggaaaggta gcgcttgtga ccggagcggg aagagggatc ggcgcagcga
     1381 tcgcagaaac acttgcaagg gaaggtgcgt ttgttgtcgt ggattaccgc ggatcgggac
     1441 aggcggcaca ggaactggcg gagaaaatcg gcggagtcgc catgcagtgc gatgtggcgg
     1501 attttgaagc ctgcgaaaac atgatcaaaa cgatgataga gacgtatggg catctcgata
     1561 ttttagtcaa caacgccgga atcacaaggg acggtctgct gatgaagatg agcgaggcgg
     1621 attttgaagc ggttctttcg acaaatctga agggaacatt tcatacgata cggcacgcgt
     1681 cccgctattt tttaaaacag cgttatggaa gaattgtcaa tatttcgtcc gtgtccggtg
     1741 tgatggggaa tgccgggcag gcaaattaca gtgcaagcaa ggcgggggtg atcggactga
     1801 cgaaaagtgt ggcaagggaa ctggcgtcgc gggggatcac ctgcaatgcg gtggcacccg
     1861 gttttattga gaccgatatg acggatgcca tgacagagag cgcaaaggaa gccgttcaaa
     1921 aacagattcc gctggggaga tgcggaaata caaaggatgt ggcagaactt gtgacatttc
     1981 tggcatcaga caaggccgcg tatatcaccg gtcaggtgat ctctgtggac ggcggcatgt
     2041 gtatgtaagg gagtagtaac tgaaagaaag tgaggcaggt atgagaagac gggtcgtagt
     2101 aaccggaatg ggcgtgataa cgccgatcgg gaactctgta gaagaatttt ggaatgcggt
     2161 gcgggaagaa aagacaggat ttggggaaat cacgcatttt gacgcatctg gatataaatg
     2221 ccatctggca gcggaagtaa agaattttga cgggaaaaat tatatggatc ccaaggcggc
     2281 aaaacgcatg gaactgtttt gccagtatgc ggtggcggca acgaaagagg ccatcgcgga
     2341 tgccggactt gagatggaga aggaagaccc gtatctggtg ggaacagcga tcggaagcgg
     2401 cgtcggaagt ctgcaggcga tcgagcgcga atataagaag cttctggaga agggaccggg
     2461 gcgagtcaat ccgcttctgg tgccgatgat gatctccaac atggcggcag gcaatgtgtc
     2521 gatccagttt ggcttcaagg gaaaaagcat caacgatgtg acggcttgtg cgaccggcac
     2581 gaatacgatt ggcgaggcgt tccggagcat ccagtacggg gaggcggaca tcatgattgc
     2641 aggaggtacg gagggcagca tctgtccgat cggaattgcc ggctttacgg cgctgacggc
     2701 actgtcgtcc gagagcgatc cgaagcgatg ttccctgccg tttgataagg agcggagcgg
     2761 gtttgtcatg ggagagggcg cgggaatcgt cgtgctggag gagttagagc atgcgaagca
     2821 gcgtggagcg aagatttatg cagaggtagc cggctacgga tgttcctcag atgcctacca
     2881 tattacatct ccggcggagg acggtgcggg cgcggcgcgt gcgatggaaa atgccatgaa
     2941 ggatgcgggg gtaaaaccgg acgagattcc atacatcaat gcacatggaa cggcgacaca
     3001 tctgaatgat ctgtttgaga cgcgcgcaat caggcttgcg tttggggaac atgccacgaa
     3061 gcttaaaatc aattccacaa aatcgatgat cggacatctg ctcggggctg cgggcgccgt
     3121 ggagtttgta acctgcgtca aggaaatgca ggaaaattat gtgcacgcga ccgtcggcta
     3181 ccagacaccg gatgaggaac tggatcttga ctactgcagg gaggcggtgg cgatggaggt
     3241 gccgtacgcg ctcagcaact cccttggatt cggcgggcac aatgcgagcc ttttacttaa
     3301 gagatatgga ggcgaggcgt gatggaactg gagatcggac agatcatgga gatccttccg
     3361 cacagacagc cgtttctgct gatcgatgcg gtggaagaac tggagcccgg aaaacgcgcg
     3421 gtggcgcgaa aatgcgtcag ctacaacgaa ccttatttcg gaggacattt cccgggggaa
     3481 ccggtgatgc ccggggtact gattgtcgag gcactggcgc agaccggtgc ggtggcaatc
     3541 ttaagtatgg aggaaaactg tggaaagacc gcgtattttg cggagattaa ccgtgccaga
     3601 tttaagaaaa aagtggtgcc gggggatgtg ctgaccctga cgacggagat catccggcag
     3661 aaaggaccga tcggtatcgg cagtgcgacg gcatctgtgc gcggggaggt cgtctgcacg
     3721 gcggagctta cgtttgcaat cggcagcaag gaggagtaga ggaaaaatgg cggctttgtt
     3781 tcagaagcgg aaaaaagaac cggcagaaaa aacaccggtg gaggcagcac aggcgctgca
     3841 ggagacgatt gtgtgcccgg cctgcggcag ggagatcaat aaaaaggaag cggaaaaaaa
     3901 tctgtatgtc tgctatgaat gtggcagtta tttcagggtg cgcaccagaa accgcatccg
     3961 catggtggcg gatgtcggaa cgtttacccc gtggtttgag gatctggcgg aggcgaatcc
     4021 gctggagttc ccgggatatg aggacaaagt aaaagcggca aaggaaaaga ccggactgca
     4081 tgaggcggtc accattgggc actgtaaaat atacggcgag gatgcagtgc tcggtatttg
     4141 tgacgcaaga tttctgatga gcagcatggg gcatgtgatg ggggagaaaa ttgcctgcgc
     4201 tgtggaacgc gcgacagagg aaggtctgcc ggtcatcctg ttttgctgct ccggtggcgc
     4261 cagaatgcag gaggggatcg tttccctgat gcagatggca aaaacatctg cggcattgaa
     4321 gcggcattcg gaagcggggc tattgtatat tccggtgctg acagacccga cgaccggggg
     4381 cgtgacggca agttttgcca tgcttggaga catcatcctg gcggagccgg gggcactcat
     4441 cggttttgcg ggaccgcgtg tgattgagca gacgatcggg gaaaagctgc cggaaggttt
     4501 tcagcgggca cagtttcagc tggagcacgg atttgttgac aggattgtgg agaggaagga
     4561 gctaaagctt accctgtacc gcatcttaaa gctgcatcac aaaaaagaag gttatgccaa
     4621 tttcgatccg gtgcgggagg cagactgcct cgaggcgaca gaactgatga aagagcgcgc
     4681 cacaaaggcg aaagcattgt ccgcgtggga gaaggtcaag gcagcgagaa aggtggaccg
     4741 gctggcgtct gtggactaca tagagcagat ttttgacgaa tttatggaat tacatggaga
     4801 ccggcagttc cgggacgatc cggcgattgt cggcggtgtg gcgtatctgg atggccagcc
     4861 agtcacagtg atcggtgtgc ataagggaaa agatttaaag gaatgtatgc accggaatta
     4921 tggaatgccg tcgccggagg gatatcggaa agcactccgg ctgatgaagc aggcggaaaa
     4981 atttaagcgt ccggtcatta catttgtgaa cacctccggc gccttctgcg gcatggaggc
     5041 ggaggagcgc ggacagggcg aggcgatcgc gagaaacctg tatgagatgt ccggcttaaa
     5101 ggttccggtt ctttgcatta tgatcgggga aggcggaagc ggcggtgcgc tggcactctc
     5161 tgttggaaat gaggtctgga tgcttgagaa tgcgacctac agcattttat cgccggaagg
     5221 gtttgcatcg attctctgga aggatggcag acgggcaaaa gaagcgagtg gcgtcatgaa
     5281 aattaccgcg caggatttaa aagcgctcgg cgtgattgag gagatcatac cggagtacgg
     5341 catggcggat cagccggcac tgatttccat atcgcgttac atgaagaggc atataaaaac
     5401 atttttgaaa aaacagaacg gaaaaaccgg ggatcagctc gctgcggagc gctatcagag
     5461 attccgcgtg ttttaaaagg aaggagaccc gatatgaagg taaagatcgt gggcaccgga
     5521 agtgcagtcc ccgaaaaatg tgtgacgaac gatgatctta gtcaatggat ggatacatcg
     5581 gatgagtgga tcagaagccg gacgggaatt gagaccagac atctggctgt gagagagaca
     5641 acgacagaaa tggcagtgga ggcggcgaag cgggcacttg catctgcaca tatggacgcg
     5701 gaggagctgg atctcatcat tgcggcgacg atcacgccgg accggctgat cccgaacctt
     5761 gcctgcgagg tgcagagcgc actcggggca tgccgggcgg tcgcctttga cgtcagtgcc
     5821 gcctgctcgg gttttttata cgcgatggat attgcacgcc actatctgga agccggcgga
     5881 agccggcggg cgctggtgat tggtgcggag acattgtcgc ggatcatgga ctggagcgac
     5941 cggagtacct gtgtgttgtt tggggatggc gcaggtgcgg cagtgctgga agcagatgag
     6001 gcggaggagc atgcgggcat cttaagcacc gtgcttggaa cggacggcgc gggcgggatg
     6061 gtattgcgct gtgacaaccg cagaaatcac aatccgtatg tgaagggcga caccgcgctc
     6121 tcgtacgtgt ccatgaacgg gcaggaggtc tataagtttg cggtgcgcac ggtgccgaaa
     6181 gtaattgccg gggcggtgga agcggcaggg ctttcgatgg atgaaatcga ttttttcctg
     6241 ctgcaccagg cgaaccttag aatcatcgag gcagtggcga aacgcctgca tcagccgatg
     6301 gaaaaatttc ccacaaatct gcagaagtat ggaaatattt cctcggcgag cgtaccgatt
     6361 ttacttgata atgtcaataa tcatggtatg ataggagaag gcagtaaaat agtactggca
     6421 ggctttggag ccggtcttac gtggggcgca gcagtgctcc gctggcaggc ggtgtaggcg
     6481 gtgtggtcgg gaacagatac tgctatgaac aggagtatga tacatgacca cggatgagac
     6541 attgaacgaa ttactggtaa ggctattcaa ggatattatg gagattgagg cgagatgtct
     6601 ggtgaatgag gaatttaagg acatcagcta caacgatttt catattatag aagccatcgg
     6661 attaaaggag ccgaaaagca tgacggcggt ggcaaagctg atggatgtca cgaccggtac
     6721 gctgacgaag gcgatggatg gcctgactga gaaggggtat gccgtaagag aacgcagcaa
     6781 gcaggacaaa cgcgtggtgt gggtgtactt gacggacaaa ggaaaagcgg catatgcgca
     6841 tcacgaagag tttcaccgca aaatgatcgc caacatcaag ggacagctga atgagcagga
     6901 gacgccgatt ttgatctata cgctggcaaa gctcgtggac tattttcaag aggaatacgg
     6961 attggaggag gaatagacat atgagcagag gagcgttggg caagaataac tgggcgctgt
     7021 ttttaatgct tctggcagga attatgctgg gcggttttat cggaaatgct gctgcaggtg
     7081 tttcgggact tagctggctg aattatgggc agagcttcgg cttcgccagc ccgattgtgc
     7141 tggatctggg cattctggtg atcacattcg gattatcgat caagattacc atcgccagca
     7201 ttatcggcgt gttgattgcg attattattt atcgtttgat ttaagttcac aagaaataga
     7261 aaagcctcgg gcaggggaaa actcctgccc gaggcttttt gcgtatgtct gatgcgggga
     7321 cataaatcgt tccggggaca ctatcctgct acaaagcttt aacaatttag cggaataaag
     7381 aaaaaaagat tgcacatgaa aacattttat actaaaatgt atctgacgaa aatattgcag
     7441 tgtatacaca gcacaatata ctaagagtaa ggagacaaaa aaaatgggat atgtagatga
     7501 agttcttgca aaagtaaaag agaaaaatgc ttccgagc
//
LOCUS       DBMDQJ010000017         7527 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4177_length_7527_cov_1.534608, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000017 DBMDQJ010000000
VERSION     DBMDQJ010000017.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4177_length_7527_cov_1.534608"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="ACZO9R_00690"
     CDS             complement(<1..1134)
                     /locus_tag="ACZO9R_00690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR0911621.1"
                     /translation="MKNYRILQIHNFYQIPGGEDVVVRNEKRLLEEHGHKVYTYYRSN
                     SELAKKGIVGKLFLPFTAVFSLKTYREVKKLIRENQIDIVHVHNTLTMVSPSVFYAAF
                     RCKVPVVQTLHNFRMLCPAGSFFRDNVICEECVQHGMGCAIRHKCYRNSKLQSFVSAA
                     ILQIHRFVGTYRRVNFICLTEFNRKKLLESLDTKKEIVNPERVYIKPNFTFAEEIVPN
                     EVRSDEEYFLFAGRVEALKGIDIAVRAFEKLPDKKLYVAGNGPMMEEMQRYVREHHME
                     NVKFLGYLQKDEMSEKFYQAKAVIMTSQCYEAFAMTIAEAYSYGVPVIAGRVGNMDGM
                     VQDGITGVKFTYNSAEDLADKISAFDAMDLSVLKKNARAFYAER"
     gene            complement(1143..2288)
                     /locus_tag="ACZO9R_00695"
     CDS             complement(1143..2288)
                     /locus_tag="ACZO9R_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911622.1"
                     /translation="MDRIKTFFRTSDWESVGVAAFYGYFAINILMKALAYDHGDNIYK
                     FFFIFAMSFWAIKIVTTRYTLREIAWIAVLLALGLGLSVITKQNTWLLLFMTIIAMKN
                     CRFELMIQMAVYIRVFCLAMLVIGSTFGVFDIGYKTTPDSSYVEIPVYSFAMNEPNTA
                     FLAVFLTLLLLLYYNYKKLNVWWFAGTSATALLFYKFTYCRTGIAVFFFVWALIIFEK
                     IAKDRWKVVLALSVPVGAVFSLCTMLFYDGGNSVMRLLNHLVSGRIYIMSSYYKTQGV
                     SLIPRAQELFYGQYYGLIDNTYMFVFLYCGAIVALFFLWCATKTLFRLYRGGYYKELV
                     MIAAFALYGVLEQFIMNGFMNPFVLLCGILLYPDLLEKKRDDVCAAE"
     gene            complement(2318..3100)
                     /locus_tag="ACZO9R_00700"
     CDS             complement(2318..3100)
                     /locus_tag="ACZO9R_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911623.1"
                     /translation="MGYVLALWGEFVKFTGGRAGLVHLLLFAAALVCCFFLGKEERHR
                     LFWPSVLVGIFFFNPVFYKYVGTRFLSGVYWRLLWMLPWTFVIAYAVVRMAYRLGKQA
                     LRIVVVAVACVCIFVTGKPVLSAETYGERSNVYELPQAAVEIADLVQSRLVDWQETLI
                     VPNELLCSVRQYSASACLLYGRNAEGFISGIGEEEQQVYEEMSSEHPDVARVTEIAKN
                     KNCRYIVFNTSFHQIPEDLTEYGYEKIAVIEDVYAVYSRIGS"
     gene            complement(3111..4211)
                     /locus_tag="ACZO9R_00705"
     CDS             complement(3111..4211)
                     /locus_tag="ACZO9R_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HIR0911624.1"
                     /translation="MSCKSLLYMVGMFVFVPVWIGLLWTDFLKIRERASQLLHAWVLG
                     FATMLAAAQLVLVPFVMMQKTLTQALLLWRIILQILAVLSFVIWLGRRAGTAETVADA
                     AAGTADGAGVQKKVCGKAGTWTIVFGILVAVLILLQAYIPARYEHSDDDDSRFVAEEV
                     SAVVHDTMNREDPITADYMYWDTGEVRKDLTSPWAMFVAMHAKEGNMAPAALSHAYLP
                     FFLILICYALYLLIGQVLFGGDWEKTFLFGIVLSVLHLAGYTSTHTLASMLLLRIWQG
                     KAVCASFALPLFFYLFYQIMKKEAWKRWIPLLYVAGVGTCMLSGIGIVTASVLLAVYG
                     VLDFCYYRNWRKTLAIWLAAVPCVIFLGYYLM"
     gene            complement(4201..5511)
                     /locus_tag="ACZO9R_00710"
     CDS             complement(4201..5511)
                     /locus_tag="ACZO9R_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911625.1"
                     /translation="MNQEYRLDREYSIAEIIQCNLRKWWLALICAAVCAAVLGGYKYK
                     SLKPFIEQSVYENVKQVTATLYVADYSTSASTERANNVIKMAQSYRAYELVQQKTGCD
                     LDYKTYQGIFDVLQGEAGDMVTIYLQYPIDTGTFSIADEDGAIAFTQEIIDATLQVGS
                     ELTGGETCFTVLDAPYPNSETRKAENYFISEDDFKGGVKKGIAAGALLGIIAEVALYT
                     FWLILYRKPKSAEEVRACLDIPVIDDVKTRKANEEEVYKKLALFLKEKQGGAEHKGVS
                     VNCMPVGYFKKDAGLKLAMSFANEKKKTLLIDLVKEPEGKEAGNSISRYVLGDESRPV
                     PTTQNSYLDVLCRDVAEEKNFDVVMNERFASYVKEMQDTYEYIVINSPNVAESADAFA
                     AGKLCDKNFVVCARGGVNNETLYRLKNEAAVQGIVLEGVLVYEL"
     gene            complement(5526..6947)
                     /locus_tag="ACZO9R_00715"
     CDS             complement(5526..6947)
                     /locus_tag="ACZO9R_00715"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR0911626.1"
                     /translation="MYQSKEKIQTVVLLLADLICFVISYFGGGYLWLVLYRNVSVQNM
                     KSELMDSFGIVLVIYMLILLFSDLEKNFIRRNLARDFWAALEASVIMIFVTALTIFLQ
                     HNNADASRGVYFCIAGMNLVLFFVAHAAIKYYLLHIYRNKRGTNQLFLVTTTDRVKDT
                     LAEIENAKDWEHRLTGIAIIDDNQIGTWYEGIPVVATYDTMFQYAKEQIVDEVFLNVP
                     YDTGESLAKVVEAFEDMGATVHITIELLKRFDDYHKNFNMMGNVPVVTFSNQYYDWKM
                     LFVKRLMDIAGAIVGLAITAVVTVFLAPPLLIESPGPLFFAQKRVGKNGRFFKIYKFR
                     SMYKDAEERKKELESQNEMNGFMFKMKDDPRITKVGKFIRKTSIDELPQFYNVLKGDM
                     SLVGTRPPTVDEFRQYESHQKRRLSAKPGITGLWQVSGRNEIKDFEDVVKLDVQYIDN
                     WSIGLDIKIILKTIKVVFEKGGE"
     gene            complement(6963..>7527)
                     /locus_tag="ACZO9R_00720"
     CDS             complement(6963..>7527)
                     /locus_tag="ACZO9R_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0911627.1"
                     /translation="YYQIFKAEGKSLADWLKSAGLTEEETECFARMEVHFQEYLRAGA
                     FPVRSMQRRMGTRIIPFAALLEGAGAGDGSEIVRESAYLRVRKLLYHIDRAESQDGSR
                     VCSGWAFAKTWDGRCLPVNIRVAAPGGEQFAAEITRRARADVAEMFKLRRVENPEFGF
                     DCVWQAAPEQRFCIYFSLGNRESIYEG"
BASE COUNT         1833 a         2324 c         1603 g         1767 t
ORIGIN      
        1 acgctcggca taaaaggctc tggcgttctt tttgagcacc gacagatcca tcgcatcaaa
       61 cgcgctgatc ttgtctgcga gatcctccgc ggaattgtag gtaaacttca caccggtaat
      121 gccgtcctgc accatgccgt ccatgtttcc gactcttccc gcaatcaccg gcacgccgta
      181 ggagtacgcc tccgcgatcg tcatcgcaaa cgcctcgtag cactgcgatg tcatgatgac
      241 cgccttcgcc tgatagaact tctccgacat ctcgtccttc tgcagatagc ccagaaactt
      301 tacattctcc atatgatgtt ccctgacata gcgctgcatc tcttccatca tcggcccgtt
      361 tcccgccaca tacagcttct tgtccggcag tttttcaaag gctctgactg caatatcgat
      421 tccctttaag gcttccacac gcccggcaaa caggaaatat tcttcgtccg agcgcacctc
      481 gttcggcacg atctcctccg cgaaagtaaa attcggcttg atgtaaaccc gttccggatt
      541 cacgatctct ttctttgtat ccagcgattc caacaacttc ttccggttga actcggtcag
      601 acagataaaa ttcaccctgc ggtatgtccc cacaaaccgg tgaatctgca ggatggcggc
      661 gctgacaaaa gactgcaact tgctgttccg gtagcacttg tgccggatgg cgcagcccat
      721 tccgtgctgc acacactcct cgcagatcac attgtcccgg aaaaaagatc ccgccggaca
      781 gagcatgcgg aaattatgca gcgtctgcac caccggcacc ttgcagcgga atgccgcgta
      841 aaagacggac gggctgacca tcgtcagcgt gttgtgtaca tgcacgatgt cgatctggtt
      901 ctcccggatc agctttttta cctcgcggta agtcttaagc gagaacaccg cggtaaacgg
      961 caggaacagc tttcccacaa tgcccttctt cgccagctcg ctgttggagc ggtagtacgt
     1021 gtagaccttg tgtccgtgtt cctcgagcag gcgcttttca ttgcgcacca ccacatcttc
     1081 cccgcccggg atctgataaa aattgtgtat ctgtaaaatg cgataattct tcatagactt
     1141 cctcattccg ccgcacatac atcatcccgc ttcttctcca gcagatccgg atagagcaga
     1201 attccgcaca gcagcacaaa cggattcata aaaccattca tgatgaactg ttccagcaca
     1261 ccgtaaagcg caaacgccgc gatcatgacc agttctttgt aatagccgcc gcggtaaagc
     1321 cgaaacagcg ttttcgtcgc acaccacagg aaaaacagcg ccacaatcgc accgcagtac
     1381 aggaacacaa acatgtacgt gttgtcaatc agcccgtaat actgtccgta aaacagctcc
     1441 tgcgcccgcg ggatcagtga gactccctgt gtcttataat agctgctcat gatatagatt
     1501 cttcccgaca ccagatggtt taaaagacgc atcacggaat tgccgccatc gtagaacagc
     1561 atcgtgcaca gactgaatac cgcaccgacc ggaactgaca gcgcgagaac aaccttccac
     1621 cggtcctttg caatcttttc aaaaataatc agtgcccaga caaagaaaaa gactgcgatt
     1681 cccgttctgc agtaggtaaa tttgtagaac aaaagcgcgg ttgccgatgt tccggcgaac
     1741 caccagacat tcagtttttt ataattataa tatagcagaa gcagcaatgt caaaaagacc
     1801 gcaagaaacg ccgtgttcgg ttcattcatg gcaaagctgt acaccggaat ctccacgtag
     1861 ctgctgtccg gcgtcgtttt gtaaccaata tcaaagaccc cgaacgttga cccgatcacc
     1921 agcatagcca gacagaagac ccggatgtac accgccatct gaatcatcag ctcgaacctg
     1981 cagttcttca tggcaatgat cgtcatgaaa agcagcagcc acgtattttg cttcgtaatc
     2041 acggagagcc cgagtccaag agccagcaac accgcaatcc acgcaatctc acggagcgtg
     2101 tagcgcgtgg tcacaatctt gatcgcccag aagctcatgg caaagataaa gaaaaatttg
     2161 tatatattat cgccgtgatc gtacgccagc gctttcatca ggatattgat cgcaaaatac
     2221 ccgtaaaacg ccgcaactcc cacgctctcc caatcacttg tccgaaaaaa tgtcttgatt
     2281 ctatccatac cgtcctttct gccttcctgc taccgcgcta tgatccgatt ctggaataca
     2341 ccgcatatac gtcttcgatc acagcaatct tttcatatcc gtattccgtc agatcctcgg
     2401 ggatctggtg aaaagaagta ttaaacacta tatatctgca gttcttattt ttcgcaatct
     2461 ccgtgacacg cgccacatcc ggatgctcgc tgctcatttc ctcgtagacc tgctgttctt
     2521 cctccccgat gccggagata aagccctccg catttcttcc gtacagcaga catgcggatg
     2581 cagaatattg tctgacactg cataaaagct cgttcggcac gataagcgtc tcctgccagt
     2641 caaccagccg gctctgcacc agatccgcaa tctcaactgc tgcctgcggc agttcgtaca
     2701 cattcgaccg ctccccgtaa gtctccgccg ataaaaccgg cttcccggtc acgaagatac
     2761 agacacatgc caccgccacc acaacaatgc gcagtgcctg ctttccaagc cggtacgcca
     2821 tccttaccac cgcataggcg atcacgaacg tccacggaag catccacagc agccgccagt
     2881 acacaccgct taagaagcgg gttcccacat atttgtagaa caccgggttg aaaaagaaaa
     2941 ttcccaccag caccgacggc cagaacaatc tgtgccgctc ctccttcccc aggaagaagc
     3001 agcagaccag cgccgccgca aacaacagca gatggacgag cccggcacgt ccgccggtaa
     3061 attttacaaa ttctccccac aaagccagta catagcccat tcttttactc ctacatcagg
     3121 taatatccta aaaagatcac acacggaacg gctgccagcc agatggcaag cgtctttctc
     3181 cagttcctgt aatagcaaaa atccagcacg ccatagacgg caagcagaac cgacgctgtc
     3241 acgataccaa ttccggaaag catgcacgtg cccacacccg ccacatagag aagcggaatc
     3301 cagcgcttcc acgcctcttt cttcatgatc tggtaaaaca gatagaaaaa cagcggaagc
     3361 gcgaagcttg cgcagactgc ctttccctgc cagatgcgca gtagcagcat ggacgccagc
     3421 gtgtgcgtcg acgtgtagcc cgcaaggtga agcacggaga gcacgattcc gaagagaaat
     3481 gtcttctccc agtctccgcc aaacagcacc tgcccgatca gcagatacag ggcatagcag
     3541 atcagaatca ggaaaaacgg cagatacgca tgcgacaacg ccgccggtgc catatttccc
     3601 tctttggcat gcattgcgac aaacatcgcc cacggcgagg tcagatcttt gcgtacctcc
     3661 ccggtatccc agtacatgta gtctgccgtg atcggatcct cccggttcat ggtgtcatgt
     3721 acgaccgccg atacttcctc cgccacaaag cgtgagtcgt catcgtcgct atgctcgtaa
     3781 cgcgcgggga tatatgcctg tagcagaatc agaaccgcca caagaatccc aaagacaatc
     3841 gtccacgttc cggctttccc acacactttt ttttgcacgc ctgcgccatc cgccgtccca
     3901 gcagccgcgt ccgcaaccgt ctccgcggtc cccgcccgcc gtcccagcca gatgacaaac
     3961 gacagcaccg caaggatctg cagaatgata cgccacagca ggagtgcctg cgtcagtgtt
     4021 ttctgcatca tcacaaacgg caccagcaca agctgtgccg cggcgagcat tgtcgcaaag
     4081 ccgagcaccc aggcgtgcag aagctgactc gcgcgctctc ttattttcaa aaaatccgtc
     4141 cacagaagac cgatccagac cggcacgaac acgaacatcc caaccatata gagcaggctc
     4201 ttacagctca tacaccagaa ctccttcaag cacgattccc tgtactgccg cctcattctt
     4261 aagacgatac agtgtctcgt tattcactcc gcctcttgca cacacaacaa agttcttgtc
     4321 acagagcttt cctgccgcaa aagcatctgc gctctctgcg acattcgggc tgttgatgac
     4381 aatgtattca taagtatcct gcatttcttt cacataggaa gcaaaacgct cgttcatgac
     4441 cacgtcaaaa ttcttctcct cagccacatc tctgcacagt acgtccagat agctgttctg
     4501 cgtcgtcggc accgggcggc tctcgtcacc cagaacataa cggctgatgg aattgcctgc
     4561 ctccttgccc tccggctcct tgaccagatc gatgagcagg gtcttctttt tctcatttgc
     4621 aaagctcatg gcaagcttta atccggcatc ctttttgaaa taaccgaccg gcatacagtt
     4681 cacactcacg cccttgtgct ccgctccgcc ctgtttttct tttaaaaaca gcgctaattt
     4741 cttatatact tcctcttcat tcgcttttct ggtcttcacg tcatcgatga ccggaatatc
     4801 cagacatgca cgcacttcct ctgcgctctt cggcttccgg tagagaatca gccagaaagt
     4861 atacagcgct acctccgcaa tgattccaag cagtgcaccc gccgcgattc cttttttcac
     4921 gccgcccttg aaatcgtcct cagagataaa gtaattctcc gcttttctgg tctcgctgtt
     4981 cggatacggt gcgtccagca cggtaaagca ggtctccccg cccgtgagct cactgcccac
     5041 ctgaagtgtc gcatcaatga tctcctgtgt aaatgcaatg gcgccgtcct catcggcgat
     5101 gctgaacgtt ccggtatcaa tcggatactg caggtagatc gtaaccatat ctcccgcttc
     5161 gccctgcagc acgtcgaaga ttccctgata cgtcttatag tccagatcgc atcccgtctt
     5221 ctgctgcacg agttcatacg cgcggtagct ctgcgccatc ttgatgacat tgttcgcgcg
     5281 ctccgtggag gcactggtgc tgtagtctgc cacatacagt gtggctgtca cctgttttac
     5341 attttcatat acactctgct cgataaaagg cttcagagat ttgtacttgt agccgccaag
     5401 caccgctgcg cacactgcgg cacagatcag cgccagccac catttgcgca gattgcactg
     5461 gatgatttcc gcaatactgt attccctgtc taagcgatat tcctgattca tatgattctc
     5521 cttccttatt ctccgccctt ctcaaaaaca accttgatcg tttttaagat aatttttatg
     5581 tcaagaccga tgctccagtt atcaatatac tgtacatcca gctttaccac atcctcaaag
     5641 tccttgattt catttcttcc gctcacctgc cagagtcctg taatacccgg cttcgcgctt
     5701 aagcggcgct tctgatggga ttcatactgc cggaactcat ccactgtcgg cggtcttgtt
     5761 ccaacgaggc tcatatctcc ctttaacaca ttgtagaact gcggaagttc atcgatgctg
     5821 gtcttgcgga taaatttgcc cacctttgtg atgcgcggat cgtctttcat cttgaacatg
     5881 aagccgttca tctcgttctg gctctccagc tccttcttgc gctcctccgc atccttgtac
     5941 atggagcgga acttatagat cttgaagaag cggccgttct ttcccacacg cttctgtgcg
     6001 aaaaacagcg gtcccggcga ttcgatcaga agcggcggcg ctaaaaatac cgtaacaacc
     6061 gcggtgatcg ccagaccgac gatcgctccc gcgatatcca tcagccgctt cacaaacagc
     6121 atcttccagt cataatactg attggaaaac gtcacaactg gaacatttcc catcatatta
     6181 aaattcttgt gataatcgtc aaaccgtttc agcagctcga tcgtgatatg cacggttgct
     6241 cccatatcct cgaacgcttc caccactttt gccagggatt ccccggtatc gtacggaacg
     6301 tttaagaaca cttcgtccac aatctgttcc ttcgcatact ggaacatggt gtcgtaggtc
     6361 gccacgaccg gaattccctc ataccaggtt ccgatctggt tgtcgtcgat gatcgcaatg
     6421 ccggtcagac ggtgctccca gtcttttgca ttctcgatct ctgcaagcgt atccttcacg
     6481 cggtccgtcg tggtgacaag aaacagctgg ttcgtccccc gcttgttgcg gtagatgtgc
     6541 agcagataat acttgatcgc cgcgtgcgcc acgaagaaca gcaccaggtt catccctgcg
     6601 atacagaaat atacgcctcg ggaggcatcc gcattgttat gctgcaaaaa gatcgtcagc
     6661 gctgtcacaa aaatcatgat gacgctcgcc tcaagcgccg cccagaaatc cctggcaagg
     6721 ttgcgccgga taaaattctt ctccagatcc gagaacagca ggatcagcat gtagatcaca
     6781 agcacaatgc caaagctgtc catcagctcg gacttcatgt tctgtacgga cacgttccgg
     6841 tatagaacca gccacagata gcccccgccg aaataactga tgacaaagca gatcagatcc
     6901 gccagaagca gcacgactgt ctgaatcttt tcttttgatt ggtacataat aaaccctctc
     6961 ttttatccct catagatgct ttcccggttg cccagggaaa agtatatgca aaagcgctgc
     7021 tccggcgccg cctgccacac acagtcaaaa ccgaactccg gattttccac ccggcggagt
     7081 ttaaacatct ctgccacatc cgcccgtgct cttcttgtaa tctccgccgc aaactgctct
     7141 ccgcccggcg ccgccacccg gatattgact ggaaggcatc tcccgtccca ggtctttgca
     7201 aacgcccatc cggaacagac tctgctgccg tcctggctct ccgcccggtc gatatggtag
     7261 agcagctttc tcacgcgcag atacgccgac tctctcacaa tctcgctgcc gtccccggct
     7321 cccgctccct caagcagcgc cgcaaacgga atgattctgg ttcccatccg ccgctgcatg
     7381 ctgcgcaccg gaaacgcccc ggctcttaaa tactcctgaa aatgcacttc catccgcgca
     7441 aagcattctg tctcttcctc cgtgagaccg gcagacttca gccagtccgc aagacttttt
     7501 ccctctgcct taaaaatctg gtaatag
//
LOCUS       DBMDQJ010000018         7248 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4334_length_7248_cov_1.481741, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000018 DBMDQJ010000000
VERSION     DBMDQJ010000018.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7248)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7248)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7248
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_7248_cov_1.481741"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="ACZO9R_00725"
     CDS             complement(<1..1099)
                     /locus_tag="ACZO9R_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911628.1"
                     /translation="MDKKENQPREKKGMPLAPVFLLGATVLLLIEMYFIVNLPRVYLV
                     QIILGAAILACTYVFIAALLKEIHKTGEQVKDEYERMLRGEKASYLMLKKISEQLEAV
                     DRNTREPGEEIITAQKAIAKVTITRSRENADALMNSNDRVVDNLLQLKESMDAGFEQF
                     AGKQQNMMDDSVKELVLKQQEFAEKLKEITAGLKEITEGLQAVKETVAEEKAQTLAAI
                     ESRMDELVSQNAALESRIAESTAAAAAQKPDMEAEPELVMEPQPEEVMEPQPEVLTES
                     EPDMEAEPELVMEPQPEEVMEPEADFSMPDTPEEELPVVDAETVTDDTPVMEEYEEPI
                     VDSDAIVEEEDAAAATDDQNEAEAESGEIDSF"
     gene            1282..2631
                     /locus_tag="ACZO9R_00730"
     CDS             1282..2631
                     /locus_tag="ACZO9R_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0911629.1"
                     /translation="MNETSSKNEITSGIIWKQLLLFFFPILLGSFFQQLYNTIDAVIV
                     GRFVGKNALAAVAGSSGMVINLLVNFFTGLTAGASVIVSQFFGSGDRKKVDDSIHTIY
                     AFSIIGSVIMTLIGITAAPALLRLMNTPEELMSDSILYLRIYFAGIFFVFIYNTGAGL
                     LRALGDSRRPLYCLMICCVINIVLDLLLIVGLGLGVAGAAIATLLSQGISAVIVTVFL
                     MRQKDLCDFSLKDIRIHPRLLKSELLLGLPGGFQYAMYSISNMIIQSALNKFGTDTVA
                     AWAAYGKLDAIVWMVSGAFGIAITTFVGQNYGAGKYDRVRKSIRVCIGMDFAAAILLM
                     LVLVCFRIPLFHIFVTDENVVEIGLKMMATMAPFYWLFVFTEVFSGALRGMGDVVVPM
                     LITTGGICLFRVVWIFGVVALFPTLTVTLLNYPVSWVLTSVLFLFYYHFRMKKLTAH"
     gene            complement(2546..3487)
                     /locus_tag="ACZO9R_00735"
     CDS             complement(2546..3487)
                     /locus_tag="ACZO9R_00735"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0911630.1"
                     /translation="MQQGEHEIISDDGLMQLYFTQVDASNELLPAHWHEHLEMICMQH
                     GAMTAYINETSYELQQGDILVVNPRDIHYTHVHGDGHYYLLQIPPEHLKRVSEDWRGL
                     HFGEYVPYSEETASVNCRMSQKLEEMKCLQEQAADGTHLLFLAAVYKLLYLLYTKDAA
                     TVDHVLRHRSRRDFERIEQSMQYVKRNYTNPITLKETADELSLTPEYFCRLFKKYTGQ
                     TFLTYVNQIRLQYFHSDLLQTDESITYLMNKNGITNYKVFLRSFKETYGMPPGQLRKK
                     QREVRDAGAISARSAFSSGNDSKTETERMLKPRTPDN"
     gene            3711..6128
                     /locus_tag="ACZO9R_00740"
     CDS             3711..6128
                     /locus_tag="ACZO9R_00740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR0911631.1"
                     /translation="MKKWTRVMYQPNRPLKEGCYVTASKEHISLSRKAAAEGMVLLKN
                     EHNLLPLKAGSRIALFGKGSFDYVKGGGGSGDVTVSYVHNLYDGFQMLHDAVSLYEPL
                     CDFYRANVNEQYDQGSVPGMTTEPALSAELLAGASSYTDTAVIVLSRFSGEGWDRSSI
                     EYNGEYNPWETETSMPKISGEIFPDGDFYLTKEESAMIAQVKQHFSRIAVVLNIGGII
                     DTSWIKNDDQISSALIAWQGGMEGGLATAELLCGKISPSGKLPDTFAASVDDYPSTAD
                     FHESVDYVDYTEDIYVGYRYFETFGDAAKRVCYPFGFGLSYTQFSLTVQSVTETAQAI
                     RAFVLVTNTGDYSGKEVVQMYYSAPQGLLQKPARELSAFCKTRTLLPGESQLLTLETA
                     KDSMASYDDLGKIFKSSYILEKGDYHFYIGTSVRDVIALDYVMTLNTDLLVEQLSSKA
                     APTSLKKRLLADGSYEELPQRTANDPNACVFEKMVPGTEEALAPATRGRASFLLLNSY
                     KENAHPLIDVYEGKISLDEFTAQLDDDDLIHLLGGQPNTSVANTYGYGNLPEYGVPNI
                     MTADGPAGLRIAPQCEVCTTAWPCATLLASTWNPALIEQVGTAGAAEVKENNIAVWLT
                     PAVNIHRNPLCGRNFEYYSEDPLLAGKMGAAMVRGIQSQHIAAAVKHFACNNKETNRK
                     HSDSRVSERALREIYLKAFEIIVKEADPWVIMSAYNMINGHRASENHDLLEGILRDEW
                     HFHGMVTSDWWTRGEHYKEIKAGNDVKMACGFPERVKKAMELGELDRSDLERCAKRVL
                     DLILKID"
     gene            complement(6311..>7248)
                     /locus_tag="ACZO9R_00745"
     CDS             complement(6311..>7248)
                     /locus_tag="ACZO9R_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0911632.1"
                     /translation="FMYNILSTMFTSIGESKIPLGLLIFSSVLNIIMDLWMVAGLGLG
                     VFGAALATLIAQGISAVFSFLIFFYRMRQYKSPFHWFDGQELQYMLRIAIPSVLQQST
                     VSIGMMIVQAVVNPFGTQALAGYSATMRVENVFSLIFVSIGNAVSPYVSQNLGAQKIG
                     RIKKGYHAALVLDLCFAVLAFIIIETLHTQISSLFLGKDGTAFAYQVSGDYMRWLGYF
                     FIFMGIKMATDGVLRGLGIMRPFLIANMVNLAIRLSVALIFAPRYGIAFVWLAVPAGW
                     LANFLISYAALRKSWPDDKIAASCNGCMDSAETKK"
BASE COUNT         1724 a         2003 c         1661 g         1860 t
ORIGIN      
        1 cgaaactgtc tatctccccg ctctctgcct cagcctcatt ctgatcgtct gtggcagctg
       61 ccgcatcttc ctcttccaca atcgcgtcgg aatctacgat cggttcctca tactcttcca
      121 tcaccggggt gtcatccgtc accgtctctg catctacaac cggcagttcc tcctccggtg
      181 tatccggcat gctaaaatcc gcttccggct ccatgacctc ttccggctgt ggttccatga
      241 ccagctccgg ctccgcttcc atatccggct ctgattctgt cagcacttcc ggctgcggct
      301 ccatgacctc ttccggctgt ggttccatga ccagctccgg ctccgcttcc atatccggct
      361 tctgcgctgc tgcagctgcc gtgctttctg caattctgct ctccaacgcc gcattctggc
      421 ttaccagttc gtccatccgg ctctcaatag cggcaagcgt ctgtgccttt tcctcggcaa
      481 ccgtctcctt cactgcctga agtccctcag taatctcctt aagccctgcg gtaatctcct
      541 ttaatttctc agcaaattcc tgctgcttta agacgagttc cttaacggaa tcatccatca
      601 tgttctgctg ctttccggca aactgctcga atccagcatc catgctctcc tttaactgta
      661 agagattgtc cactacgcgg tcgttggaat tcataagtgc atctgcattt tccctgcttc
      721 tggtaatggt caccttggcg atcgccttct gcgctgtgat gatctcttcg cccggctccc
      781 tggtattgcg gtcaaccgcc tccagctgct ccgagatttt cttgagcatc agatatgacg
      841 ccttttcccc gcgaagcatc ctctcatatt cgtcttttac ctgctccccg gtcttatgta
      901 tctcctttaa taaggcagcg atgaatacat aagtacaggc gagaattgct gctcccagaa
      961 tgatctgcac cagatatacc cggggcaggt tgacgataaa atacatttca atcaacagca
     1021 gcaccgttgc tcccagcaaa aagaccggcg caagcggcat accctttttc tcacgtggct
     1081 gattctcttt cttatccata gtgtgtcctc cgtggctttc ctgtccatcc ctggcaacag
     1141 gcattttata gtgttatatt atcacagaac gcaaaaaaca taaacggatt ttcttgttat
     1201 tttatgctgc agattttata atatatttcg gacttatttt cacatttttt atacctttac
     1261 aacagatatg gagattatag catgaacgaa acctcttcca agaacgaaat cacatccggt
     1321 atcatctgga aacaacttct tttatttttc tttcctattt tactgggttc tttttttcaa
     1381 cagctttaca acacgatcga cgccgtcatt gtgggacgct ttgtcgggaa aaatgcactt
     1441 gccgcggtcg ccggttcctc cggaatggtc atcaacctgc tggtcaactt ttttaccggg
     1501 ctgactgccg gtgcttccgt catcgtctcc cagttcttcg gatccgggga ccgcaaaaag
     1561 gtggacgaca gtattcatac gatctatgcc ttctccatca tcggcagcgt catcatgaca
     1621 ctgatcggca tcactgctgc cccggcgctt cttcgactga tgaatacgcc ggaagaactg
     1681 atgagtgatt ccattctcta tctgcgcatc tattttgccg gtatcttctt cgtgtttatt
     1741 tacaataccg gagcagggct gctccgcgca cttggagatt ccagacgtcc cctgtactgt
     1801 ctgatgatct gctgcgtcat caatattgtt ctcgacctgc tcctgatcgt cgggctggga
     1861 ctcggtgttg ccggtgcggc gatcgccacg cttctctcgc aggggatcag cgccgtgatc
     1921 gttaccgtct ttctgatgcg ccagaaagat ctctgcgatt tttccttaaa agacatccgt
     1981 atccacccga gacttctcaa atcagagctg cttttgggac tgccgggtgg atttcagtat
     2041 gccatgtaca gcatttccaa catgattatc cagtccgcgc tcaacaaatt cggaaccgac
     2101 acggttgccg catgggcggc ttacggaaaa ctggacgcga tcgtctggat ggtcagcggt
     2161 gctttcggta ttgccatcac aactttcgtc ggtcagaact acggcgccgg gaagtatgac
     2221 cgcgtcagaa aaagtatccg cgtgtgtatc ggcatggact ttgccgccgc catccttctg
     2281 atgctggtgc tcgtatgctt ccgcattccg ctcttccaca tttttgtgac cgatgaaaat
     2341 gtcgtggaga tcggactgaa aatgatggcg accatggctc cattttactg gctgttcgtc
     2401 tttaccgagg tcttctccgg tgcgctgcgc ggcatgggtg atgtcgtcgt tccaatgctc
     2461 atcacaaccg gcggtatctg cctgttccgt gtggtatgga ttttcggagt ggttgccctc
     2521 ttcccgacgc tcaccgtaac gctcctcaat tatccggtgt cctgggtttt aacatccgtt
     2581 ctgtttctgt tttactatca tttccggatg aaaaagctga ccgcgcactg attgctccgg
     2641 catctcttac ttctctctgc tttttgcgaa gctgaccggg cggcatgcca taggtttcct
     2701 taaagctgcg cagaaacacc ttatagttgg taattccatt tttattcatt aaataagtaa
     2761 tactctcgtc cgtctgcagc agatcactgt gaaaatactg caggcggatt tgatttacat
     2821 aagtcaaaaa tgtctggccg gtgtactttt taaacagacg gcaaaaatat tccggcgtaa
     2881 gtgacagttc atctgccgtc tcttttaaag taatcgggtt cgtgtaattt cttttcacat
     2941 actgcatgct ctgctcgatc cgctcaaaat ctctcctgga gcggtggcgc agcacatgat
     3001 cgaccgtggc cgcatccttt gtatacagca gatacagcag cttataaacc gctgcaagaa
     3061 atagcagatg cgtaccgtcc gccgcctgct cctgcaggca cttcatttct tccagcttct
     3121 gtgacatccg gcaattcacg gatgcggtct cctccgaata cggaacatat tccccgaaat
     3181 gcagtccccg ccagtcctca ctgacgcgct ttaaatgctc cggcggaatc tgcagcagat
     3241 agtaatgccc gtccccgtgg acatgcgtgt agtgaatgtc tctcggattg acaacgagaa
     3301 tatctccctg ctgcagttca tacgacgtct cattgatgta cgctgtcatt gccccgtgct
     3361 gcatacagat catttccaga tgctcatgcc agtgtgccgg cagcaattca ttggacgcgt
     3421 cgacctgcgt aaaatagagc tgcatcagcc cgtcatcgga tataatctca tgttccccct
     3481 gctgcactcc cgctccctct ctttcctgct ctctccgtgt ttttcacatg cacggtttct
     3541 ctttctacca tcataccttc agacggaaaa aatatcaata tcgacccata tttctgccaa
     3601 aattcgatct ataacaaacg gaattatctg cttataatac gaacatagac aatgttccat
     3661 gatggaaaat atcaaaataa tatcaggcgt aagccggaaa agaggtcaaa atgaagaaat
     3721 ggaccagagt gatgtatcag ccaaaccgtc cgttaaaaga gggctgttac gtgactgctt
     3781 ccaaagaaca catctcactt tccaggaaag ctgcagcaga gggtatggtt ctcttaaaga
     3841 acgagcacaa tctgctgccg ttaaaagcag gcagccggat tgcactcttt ggaaaaggca
     3901 gttttgatta tgtcaaggga ggcggcggca gcggcgatgt gaccgtatcg tatgtgcaca
     3961 atctctacga tggctttcag atgctccacg atgccgtctc cctctacgag ccgctgtgcg
     4021 atttctaccg cgccaatgtg aacgaacagt acgatcaggg ttccgttccc ggcatgacga
     4081 cagagccggc gctctccgca gagcttctcg ccggcgccag ctcctacacc gacacggctg
     4141 tgatcgtatt gagccgtttt tccggagaag gctgggaccg ctccagcatc gaatacaacg
     4201 gagaatataa tccgtgggag acagagacca gcatgccgaa aatctccgga gagatcttcc
     4261 cggacggcga cttctatctc acaaaggaag aatccgccat gatcgcgcag gtaaagcagc
     4321 atttttccag aattgcggtc gtcctgaata tcggcggcat catcgatacc tcctggatca
     4381 aaaatgacga tcagatctcc tccgcactta tcgcatggca gggcggcatg gagggcggac
     4441 ttgccacggc ggaactgcta tgcggaaaga tatctccgtc cggcaagctt ccggacacct
     4501 tcgccgcttc cgtggacgac tatccttcca ccgctgattt ccacgaatcg gttgactatg
     4561 tggactatac tgaggatatc tatgtgggct accgctattt tgagactttt ggggatgcgg
     4621 caaaacgcgt atgctatccg ttcggattcg gactttccta cacacagttt tcccttacgg
     4681 tgcagagtgt gaccgaaacc gcgcaggcca tccgcgcttt tgtccttgtc acaaacaccg
     4741 gagattacag tggaaaagaa gtcgtgcaga tgtattacag tgcgccgcag ggacttttgc
     4801 agaaaccggc gcgcgaactc tccgcttttt gcaagacaag aacacttctc ccgggcgaaa
     4861 gccagcttct gacgctcgag acagcgaagg actccatggc ttcctacgat gatctgggaa
     4921 aaatttttaa atcctcctac atactggaaa agggcgacta ccatttttac atcggaactt
     4981 ccgtccggga tgtcattgcg ctggattatg tcatgacgct taacaccgac ctgctggtag
     5041 agcagctctc ctccaaagcc gcacctacaa gcttgaagaa gcgcctgctc gcagacggaa
     5101 gctacgaaga acttccgcag agaactgcca acgatccaaa tgcctgtgtt tttgaaaaaa
     5161 tggtaccggg caccgaggag gctctcgccc cggcaacacg cggacgcgca tccttccttc
     5221 tgctgaattc ctataaggaa aacgcacatc cgctgatcga tgtttacgag ggaaagattt
     5281 ccctggatga atttaccgcc cagctggacg acgatgatct gattcatctg ctcggcggcc
     5341 agccaaatac ctctgtcgcg aatacctacg gctacggcaa tcttccggaa tacggcgttc
     5401 caaacatcat gaccgccgac ggtcctgccg gtctgcgcat cgcaccgcag tgtgaggtct
     5461 gcacaacggc ttggccatgc gccacactgc tcgcctccac ctggaaccct gccctgatcg
     5521 agcaggtcgg aaccgcaggc gccgcggaag taaaagagaa caacatcgcc gtatggctga
     5581 cgccggcggt caacattcac cgcaatcctc tttgcggaag aaattttgaa tactattcgg
     5641 aagatccgct gcttgccggc aaaatgggcg cagccatggt gcgcggcatc cagtcgcagc
     5701 acatcgccgc agccgtgaag cattttgcat gcaacaacaa ggagaccaac cgcaagcaca
     5761 gcgactcccg cgtctccgag cgcgcactcc gcgagatcta cttaaaggca tttgaaatca
     5821 tcgtgaagga agccgatccg tgggtcatca tgtccgctta caatatgatc aacggccacc
     5881 gcgcttcgga aaatcacgat ctgctcgaag gcatcctgcg tgacgagtgg cattttcacg
     5941 gaatggtaac ctccgactgg tggacccgtg gtgagcatta caaagagatc aaagccggaa
     6001 atgacgtgaa gatggcgtgc ggtttcccgg aacgcgtaaa aaaagcgatg gaactgggtg
     6061 aactggatcg aagtgatctg gagcgctgcg caaagcgcgt acttgatctg atcttaaaga
     6121 tcgattagaa aactcattct tcctgctgta gatcaaaaga cggaagcctc tcacagagac
     6181 ttccgccttt ttgcttttcg actatagata ttttccgata tggattcaac cgcagcggct
     6241 cctgcttcct ccccgcctct ctggaagttt ttgttgcagt caatccgtgc ggttccattg
     6301 gcagctgcag ctacttcttc gtttctgcag aatccataca accgttgcag gatgcagcga
     6361 ttttatcatc cggccacgat tttctaagcg ccgcatagga aatcagaaag tttgccagcc
     6421 agcccgccgg tacggcaagc cagacaaatg caatgccata acgcggtgca aaaatcaaag
     6481 caaccgacag acggatcgca agattcacca tattagctat aagaaatgga cgcatgattc
     6541 caagtccacg cagaactcca tccgttgcca tcttaatgcc catgaaaata aagaaatagc
     6601 caagccatct catgtaatcc ccggatacct gatatgcgaa agcagttccg tcttttccca
     6661 gaaatagtga agaaatctgg gtgtgcagtg tttcaataat tataaaagcc aggactgcaa
     6721 agcagagatc taacaccagt gcagcgtgat agcctttttt gatacgcccg attttctgag
     6781 caccaaggtt ctgggacaca tacggcgaaa ctgcattgcc gatggacaca aatatcagtg
     6841 agaaaacatt ctctactctc attgttgccg aataacccgc aagtgcctgt gtcccaaatg
     6901 ggtttacaac tgcctgtacg atcatcatgc ctattgatac tgtggactgc tggagaactg
     6961 acggtatggc aatccggagc atatactgta actcctgtcc gtcaaaccag tgaaagggac
     7021 tcttatattg gcgcatccgg taaaaaaaga tcaggaatga aaatactgca gaaatgccct
     7081 gtgcgatcag agttgcaagg gctgcaccaa atacaccaag accaagccct gccaccatcc
     7141 aaagatccat aataatattc aaaacagaag aaaatatcag cagacccaac ggaatctttg
     7201 attccccgat tgaggtgaac atcgttgaaa ggatattgta cataaata
//
LOCUS       DBMDQJ010000019         7177 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4375_length_7177_cov_1.842849, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000019 DBMDQJ010000000
VERSION     DBMDQJ010000019.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4375_length_7177_cov_1.842849"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(545..1429)
                     /locus_tag="ACZO9R_00750"
     CDS             complement(545..1429)
                     /locus_tag="ACZO9R_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911633.1"
                     /translation="MGISINAPGSVSSQASMTGTLSIRKSTNTFEQMAGVSSSGRTSK
                     KQLNYNHRDISGQLLRAKKPQSASAALTRAKSKVSMLQRAAASGQYDSREVADALAHA
                     RRMVRCAQLKVRNLREEEREQQSAQKENSGKSLQKEHEVKRRVAQKERQLKQKVSIEN
                     TQEVLRQKKKKNEMAQKQQRHRAQERGRIAEADFKYIKSQLEAGKGFGGGSSYCEDSG
                     FTLDISAAAMSMAELEMMAQNQIEAEAEAEVEAEMSLTDGGTLAAATAGAVPAGTGVG
                     TASDAAPAAAGVSVDVSV"
     gene            complement(1548..2942)
                     /locus_tag="ACZO9R_00755"
     CDS             complement(1548..2942)
                     /locus_tag="ACZO9R_00755"
                     /inference="COORDINATES: protein motif:HMM:NF039639.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HIR0911634.1"
                     /translation="MLKGDTGAKRTWFYENGAFVAAALAAVLWHVILSTNVGDDMVYF
                     KTLLDGNSSLGEILAHRYETWSSRMVIEAVLIPLVHCPLLWKILDIVIFTSLPVLLCG
                     LLGVTGRGRWFVTGLVLLYPFADMASAGWIATTTNYLWPLWGVLVIGMVLKQLRCGRK
                     VPVWEAAAAFLACAYAGSQEQAAVLLLLLLGMEVLHYISEKRMKQPLLYALCGIDIIS
                     LIYIFSCPGNAIRSAQEMAGRMPEFADFTFAEKLYMGLANVERIFIAELDPVYCVVAA
                     VLVLLVYRKTGDYRKTLLAGIPALLLFGQAVVRVSHPSLKKVFVRPEQTTHWDWHALV
                     TYMPLVFLVLSVWGILYALWQLADGVWKHYLWTAFLLAGGFAMGVVMGFSPTIYASAN
                     RPYLYLYFVLIYVCADAVWNMGGMKAVWKTGSSDAVRKMPVPEKLMFTVLGLLVLANI
                     LDVTRMCWLLGRIL"
     gene            complement(2936..4933)
                     /gene="metG"
                     /locus_tag="ACZO9R_00760"
     CDS             complement(2936..4933)
                     /gene="metG"
                     /locus_tag="ACZO9R_00760"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR0911635.1"
                     /translation="MSDKGKFYMTTAIAYTSGKPHIGNTYEIVLADAIARYKRLEGYD
                     VYFQTGTDEHGQKIQEKAEKAGITPKEYVDNVAGEVKRIWDLMNTSYDNFVRTTDADH
                     EKQVQKIFKKLYDQGDIYKGSYEGLYCTPCESFWTESQLVDGKCPDCGREVKPAKEEA
                     YFFKMSKYADRLIEHINTHPEFIQPVSRKNEMMNNFLLPGLQDLCVSRTSFSWGIPVD
                     FDPKHVVYVWLDALTNYITKIGYDADGNSSDLFKKNWPADLHLIGKDIIRFHTIYWPI
                     FLMALDLPLPKQVFGHPWLLQGGDKMSKSKGNVIYADDMVDLFGVDATRYFVLHEMPF
                     ENDGVITWELVVERFNSDLANILGNLVNRTVSMSNKYFGGVVRSTGVTEDVDEDLKTV
                     VTGARDRVAQKMKDLRVADAISEVFGVFRRCNKYIDETAPWVLAKDEAKSDRLAEVLY
                     NLAESITVGASLLYSFLPETAEKIVAQLHTQLRGFEELNQFGLYENGTKVTGTPEILF
                     ARLDAKDVMPKVEAIREAQKKEFEAEQRALGEETPAEEKAEDVIDIEAKEEITYDDFM
                     KLQFQVGEIISCEAVEKSKKLLCSQVRVGSEVKQIVSGIRKYYTPEEMVGKKVMVLVN
                     LKPAKLAGVLSEGMLLCAEDAEGNLALMTPEKPMPAGAEIC"
     gene            complement(5348..6496)
                     /locus_tag="ACZO9R_00765"
     CDS             complement(5348..6496)
                     /locus_tag="ACZO9R_00765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012516.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR0911636.1"
                     /translation="MEIRCVYTEGNQNVIQEAVDRLPSAGGTVIVPRGNWKSGAIHLK
                     SNVKLYLEEGCVIHFSSCMEDYLPPVFTRWEGVECYNYSPLIYAADCENVTICGTGVL
                     DGAGSAWWHWKKLQQNAADRLIRAESQGIVVEERVFATREDALRPSFIQFINCKHVTL
                     EDFTIEDGPQWTIHPVYCEDVVVRGVTVNTKGPNTDGCNPDSCRKVLIEDCTFDTGDD
                     CIAINSGMNEDGWRVGRPCEQIEVKNCRFIGGHAAVAIGSGMSGGICDIRIHDCVVRG
                     TERGIRIKSMRGRGGYVKRINVERMQMDEIEKEAIEVSMNYGSSTAVPVSQKAPEFSE
                     IRFAHIRGNHAAVGVSLCGLPESPLRKLTLEDVVIDAEDPLREEYVEK"
     gene            complement(6609..>7177)
                     /locus_tag="ACZO9R_00770"
     CDS             complement(6609..>7177)
                     /locus_tag="ACZO9R_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR0911637.1"
                     /translation="LFGAEGLIYASAYVTVFNILLWTVGFGMITGTVEPKEVVRTVCT
                     NPVLIAVVIGLVIYLCQIPVPEVIEQPLALIGNMNTPLSMIITGMMIAGSNLAQMLCD
                     RRILSVMGIRMLLIPAVCFALFFACGFSGMAAGVVLLLEACPSAAITSVFAVQYSYDE
                     EFAAGSVVITTLLSIVILPVCAMLLPGM"
BASE COUNT         1626 a         2223 c         1558 g         1770 t
ORIGIN      
        1 acattctata gatttcaggt agaactgcat aggaaagatt tattgaaaaa aatgtgtgca
       61 gttcttccag ccggatgact acatctgatt tcgtgtctct tccacgtttg gactctcccg
      121 gagagtgtcc gacaagccac agacaatgag cggactttct agtgtcccta atggcaccgc
      181 ttgacagaca atattaaacc gcgcgaaccg aaactcgttg gcatctgcta ttgaaaatag
      241 ggcagatgcc aattcagaca tcggcttcgc gcggttatgt tctgctcatt ggtgccaata
      301 agggacacac aaaagtccta aaattgtctt ctggcttgcg gacacactcc gcggagtgct
      361 ccaaacttgg aaagacacga atcatcgcag atgtagccac cggctggtta gaaatgccac
      421 attttttgaa caaaatattt cctgtgcatt ctacctgaaa tcgaaaagtg tgattttctt
      481 ataacatgac ttagccttca aaggacaccg cctgcttcat ggggcagtgt ccctcgccgt
      541 tttgttacac tgacacatcc acagatacgc cggctgcggc tggcgctgcg tccgatgcag
      601 ttcccactcc ggtacctgcc ggcacggctc ccgcggtcgc agctgcaagc gttcctccgt
      661 cggtaagtga catctccgct tccacttccg cctctgcctc cgcttcaatc tgattctgcg
      721 ccatcatctc gagctccgcc attgacattg cggcagcgct gatatccagc gtaaatccgc
      781 tgtcctcaca ataagaactg ccgccgccaa agcctttgcc agcctcgagc tgtgacttga
      841 tgtatttgaa atccgcttcc gcaatcctgc cccgctcctg cgcgcggtgt ctctgctgct
      901 tctgcgccat ctcattcttc tttttcttct gccgaagcac ttcctgcgta ttctcaatcg
      961 ataccttctg cttgagctga cgctctttct gtgccacacg acgcttcact tcatgttcct
     1021 tctgcaggct cttaccgctg ttttccttct gcgcagactg ctgctcgcgc tcttcctcgc
     1081 gcagattgcg cactttcagc tgcgcacagc gcaccatccg tctcgcatgc gcaagcgcat
     1141 ccgccacttc cctggaatca tactgcccgg atgccgccgc acgctgaagc atagacacct
     1201 tgctctttgc acgggtcagc gccgcagatg cactctgcgg cttcttcgcc cggagcagct
     1261 gtccggaaat atctctgtga ttataattga gctgtttttt cgaggttctc cctgaactac
     1321 tgacccccgc catctgctca aatgtatttg tgctttttcg gatggaaagt gtgcctgtca
     1381 tcgatgcctg actgctcacg cttcccggtg cattgatgct gattcccata tccacattcc
     1441 tccctgaatg tatccctgac tgaatccgtt catatggtat acatcggctg aaattggatt
     1501 tttcgttagg tttcctgcat gaaacagcgg atttctgtta taaacggtca cagaatcctt
     1561 ccaagcagcc agcacatccg ggtcacatcc agaatgtttg ccagcaccag aagcccgagt
     1621 acggtaaaca tcagtttttc cggcaccggc attttccgca cagcatccga actgcctgtc
     1681 ttccagacag ccttcatgcc gcccatgttc cacacggcat ccgcgcacac atagatcagc
     1741 acaaaataca gatagaggta cggccggttt gccgatgcgt aaattgtcgg tgaaaatccc
     1801 atgaccaccc ccatggcaaa accgcctgcc agtaaaaacg ccgtccacag gtaatgcttc
     1861 cacacgccgt ccgccagctg ccacagggcg tatagaatcc cccaaacaga cagtaccaga
     1921 aataccagcg gcatataggt taccagagca tgccagtccc agtgtgtcgt ctgttccggt
     1981 cgcacgaaca cctttttcaa ggatgggtgg ctgacacgca ccaccgcctg tccaaacagc
     2041 agcagcgccg gaatccccgc caaaagcgtc ttacggtaat cgcctgtctt acgatacaca
     2101 agcagcacaa gcactgctgc cacaacgcaa tagaccggat caagctccgc gataaagatc
     2161 cgttccacat tggcaagtcc catgtagagc ttctctgcga atgtaaaatc agcaaactcc
     2221 ggcattctcc ccgccatctc ctgcgcgctc cggattgcat ttcccggaca actgaaaata
     2281 tatatgaggg aaataatatc tatgccgcac aatgcataga gaagcggctg tttcatacgc
     2341 ttttccgaga tataatgcag cacttccatg ccaagcagca gaagcagcag caccgcagcc
     2401 tgttcctggc tgcccgcata agcacacgcc agaaacgcag ctgccgcctc ccacaccggc
     2461 acttttctgc cgcagcgaag ctgctttaac accatgccga tcaccagaac gccccagagc
     2521 ggccagagat aattggtcgt cgtcgcaatc catccggcgg atgccatatc cgcaaacgga
     2581 tagagcagca ccagtccggt tacaaaccag cgtccgcgcc cggtcacacc cagcagtccg
     2641 cagagcagca ccgggaggga cgtaaagatg acaatatcaa ggattttcca gagcagcgga
     2701 cagtgcacca gtggaattaa caccgcctcg atcaccatgc gcgatgacca tgtctcatag
     2761 cgatgtgcca ggatctcgcc gagtgaactg ttaccgtcca gaagggtttt aaaatagacc
     2821 atatcatcgc cgacattcgt ggacaatatc acatgccaaa ggacggcggc aagcgccgcc
     2881 gccacaaatg caccgttttc ataaaaccat gtccgttttg ctccggtgtc tccttttaac
     2941 atatttcagc acctgccggc atcggtttct ccggcgtcat cagcgccaga ttgccttccg
     3001 catcctcagc gcataaaagc atcccctctg agagaactcc cgcaagcttc gcaggcttta
     3061 agtttaccag taccatcact tttttaccga ccatctcttc cggcgtgtaa tacttacgga
     3121 tgccggaaac gatctgcttc acctcgctgc cgactcttac ctgggagcac agaagcttct
     3181 tcgacttctc cacagcctca caggaaatga tctctcccac ctggaactga agcttcataa
     3241 aatcatcata tgtgatttct tccttcgcct cgatgtcgat gacgtcttct gctttttctt
     3301 ctgcaggagt ctcctcgcca agcgctctct gctctgcctc aaactccttc ttctgtgcct
     3361 cgcggatcgc ttctaccttc ggcatcacat cctttgcatc cagacgtgcg aataagatct
     3421 ccggcgtccc ggtcactttc gtgccgttct catacagacc aaactgattc agttcctcaa
     3481 aaccgcggag ctgtgtatga agctgtgcca caatcttctc tgcggtctcc ggcaggaagc
     3541 tgtacagcag ggatgccccc accgtaatcg actctgccag attataaagt acctctgcca
     3601 gacggtcgga tttcgcctcg tccttcgcaa gtacccacgg tgccgtctca tcgatatatt
     3661 tgttgcagcg gcggaacacg ccgaatacct cagaaatggc atctgccaca cgcagatcct
     3721 tcatcttctg tgccactctg tctctggctc cggtcacaac tgtctttaag tcttcgtcca
     3781 cgtcctctgt cacgcccgtg cttcttacca caccgccaaa atacttgttg ctcatggaaa
     3841 ccgtgcggtt gacgagattt ccgaggatgt tggcaagatc ggaattaaag cgctccacca
     3901 ccagttccca ggtaatcaca ccgtcattct caaacggcat ctcatgcagt acaaagtaac
     3961 gggttgcatc cacgccaaac agatccacca tatcgtccgc atagatcaca tttcccttgg
     4021 atttgctcat cttgtctccg ccctgtaaga gccacggatg tccgaatacc tgcttcggca
     4081 gcgggagatc cagcgccatc aggaaaatcg gccagtagat cgtatggaaa cggatgatgt
     4141 ccttgccgat cagatgcaga tctgccggcc agttcttttt aaataaatcg gaagaattgc
     4201 cgtccgcatc ataaccgatc ttcgtgatat agttggtcag cgcgtccagc catacataaa
     4261 ccacatgctt cgggtcaaaa tctaccggaa ttccccagga aaatgacgtt ctggaaacac
     4321 acagatcctg caatcccgga agcaggaaat tgttcatcat ctcgtttttt ctggataccg
     4381 gctgaatgaa ctccggatgg gtgttgatat gctcgatcag gcggtccgca tatttgctca
     4441 tcttgaagaa atatgcctcc tccttcgccg gttttacctc tctgccgcag tccgggcact
     4501 tgccgtccac cagctgggac tctgtccaga aggactcaca cggcgtacag taaagtccct
     4561 cgtaagatcc cttgtagata tctccctgat cgtacagctt cttaaaaatc ttctgcacct
     4621 gtttctcatg gtctgcatcg gtcgtgcgga caaagttatc gtaggacgtg ttcatcagat
     4681 cccagatgcg ctttacctca cctgccacgt tatctacata ctcttttggc gtaatgcctg
     4741 ctttttctgc cttctcctga atcttctggc catgctcatc tgttccggtc tggaaataaa
     4801 cgtcgtagcc ctccagtctc ttataacgcg cgatcgcatc tgcgagcacg atctcatacg
     4861 tatttccgat atgcggcttg ccggatgtgt aggcaatcgc ggtcgtcatg taaaattttc
     4921 ccttgtcact cattttgtct gctccttcct aataaaacaa aaaaatcccg tcttcggggc
     4981 tgtatcagcc accagaagac gggataactc ccgtggtacc acttctattc acacagaatc
     5041 tgcactctgt gcctctgcgg atatcgcaac gaatatcccc cgctgtaaca ggcggtcccg
     5101 tcagaactta tcgctgtcct tagtaacgtc cagtgttcag tcctgccact ccaaagccat
     5161 cttccatcca cccttcctca tttcctcgca gcaagctgcg accgcgcagc tggaaatctc
     5221 tctgtaaagt atctgcagat gtactctctt cttcctcgtg tttgtgtgta tcgacttttc
     5281 taagattagc accaaacctg cggaaagtca agcgttcgta agcacaggag ccccgagaaa
     5341 aagtatttta cttttccaca tactcctccc gcagcgggtc ttctgcatcg atcaccacgt
     5401 cttccagggt aagttttcgc agcggacttt ccggcagtcc gcagagcgag acaccgacgg
     5461 ctgcatggtt tccccttata tgggcaaaac ggatctccga aaattccggc gccttctgcg
     5521 acaccggcac cgccgtgctg gaaccataat tcatgctcac ttcgatcgct tccttttcta
     5581 tctcatccat ctgcatccgt tccacattga tccgctttac atagccaccg cgcccgcgca
     5641 tggatttgat gcggatgccg cgctcggtcc ctctgaccac gcagtcatgg atccggatgt
     5701 cacaaatgcc gccggacatt ccgcttccga ttgccactgc cgcgtgtccg cctataaagc
     5761 ggcaattttt tacctctatc tgttcgcagg ggcgccctac acgccatccg tcctcgttca
     5821 tgccggaatt gatagcaatg cagtcatcgc cggtatcaaa ggtgcagtcc tctatcagca
     5881 cttttctaca tgaatccgga ttgcagccat cggtgttcgg tcccttggtg ttcaccgtca
     5941 ccccgcgcac cacaacatcc tcacaataca ccgggtggat cgtccactgc ggtccgtcct
     6001 cgatcgtaaa atcttccagc gttacatgct tacaatttat gaactggatg aacgagggac
     6061 ggagcgcatc ctccctggtc gcaaatacgc gctcttctac cacaatcccc tgactttccg
     6121 cccggatcag gcgatccgcc gcattctgct ggagcttttt ccagtgccac cacgcgcttc
     6181 ccgcaccgtc taacacaccg gtgccgcaga ttgtaacatt ctcacagtct gccgcgtaga
     6241 taagcggcga ataattgtaa cattccacgc cttcccagcg tgtaaatacc ggcggaaggt
     6301 aatcctccat gcaggaactg aaatgaatca cgcatccctc ttccagatac agtttcacat
     6361 tgcttttcag atgaattgca ccgcttttcc aattgccgcg cggcacgatt accgtgcctc
     6421 ccgccgaggg cagacgatcc accgcctcct gaatgacatt ctgatttcct tctgtataca
     6481 cacatctgat ttccatgtga ctctctcctt tttcttatcc tgcctgcttc ccatgccatc
     6541 tccgggttct tccgccatat ggcttcttcc cgctatgtag cttccatatg gcttcttccc
     6601 gcagttttct acattcccgg cagaagcatg gcgcataccg gcaggatcac aatgctgagc
     6661 agggtcgtaa tgaccacgct gcccgccgca aattcttcat catacgaata ctgcaccgca
     6721 aagacggagg tgatcgccgc gctcgggcat gcttccagca gcagcaccac gcctgcagcc
     6781 atcccggaaa agccgcatgc aaaaaacagc gcgaagcaca cggcagggat cagcagcatg
     6841 cggattccca tcacagataa aatgcgccgg tcgcaaagca tctgcgccag attgcttccg
     6901 gcgatcatca ttccggtaat aatcatggag agcggcgtat tcatatttcc gatcagcgcg
     6961 agcggctgtt cgatcacctc cggcacagga atctggcaca ggtagatcac cagtccgatc
     7021 accacggcaa tcagcaccgg atttgtgcag actgtgcgga cgacctcttt cggctccacg
     7081 gttcctgtaa tcatgccaaa tcccaccgtc cacagcagaa tgttgaacac cgtcacgtac
     7141 gcactcgcat agatcagtcc ctccgcgcca aacagcg
//
LOCUS       DBMDQJ010000020         7097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4427_length_7097_cov_1.557033, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000020 DBMDQJ010000000
VERSION     DBMDQJ010000020.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4427_length_7097_cov_1.557033"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(501..2237)
                     /locus_tag="ACZO9R_00775"
     CDS             complement(501..2237)
                     /locus_tag="ACZO9R_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0911638.1"
                     /translation="MKKKSSVIAKLIIPVAVLGCIAIMIAGVSLYSMTAVQKESNQIS
                     GEGVRATICMDEINLAFANTQKLTLALCAEPSKDLYEYVASQLTEYQSNVDSYEKELL
                     AMDHYFTADDIQLMNETFDLLTEAQGTTVELMQTAMAGDSAAAVAKANSVMTEWSDTI
                     AVNMDTLISRNDELVKQNIQEQKDLYNQNMILSLILLAISFVAFAMVVVVIIKTVVKP
                     LRKQTSELTEIIDEIKGGHGDLTKRVTVKSMDEIGQSSIGINHFIETLQNIMSNIISN
                     SNVLDGVVGNVASSVAASSDNANDISAIMEELSATMEEVSATTNSVSENTTAAEGKVQ
                     KMADQTKVMSQYAQDMKKRATELEHTATENMNNTNEMIGEITTEMNQALENSKSVEKV
                     AQLTADILSISSQTNLLALNASIEAARAGEAGKGFAVVADEIRQLADSSRETANNIQT
                     INEQVIEAVQGLVVSSEKIVGYINENILPDYRAFVQGGQQYNDDATHIDNTMAEYAGE
                     AQDILATMMEMTEAIEGISRAVEESANGVTDAATNIDSLVQSMSTVNGQMEENSTVAK
                     NLKEESAAFACV"
     gene            2390..2998
                     /locus_tag="ACZO9R_00780"
     CDS             2390..2998
                     /locus_tag="ACZO9R_00780"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR0911639.1"
                     /translation="MEEDRKKLTEKKLLSCGKREFLEKGYAKANLRSICEASGVTTGA
                     FYFSFASKEAALLRAILDPFIADSRELLSGLARMEEEHPETADDNEIQIIRYLSAHRE
                     ECIILMEKSAGSCYEHFHEEAAAMMQESFRRYYRKYLGAEPDPELIRILASMRLHGYL
                     ELVHGDYTMKERLFLARAIGIHADAGTLSLIQYLKEENDAHR"
     gene            3065..3853
                     /locus_tag="ACZO9R_00785"
     CDS             3065..3853
                     /locus_tag="ACZO9R_00785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0911640.1"
                     /translation="MNQKPNYGNWVPEKLLYLTFGGAALFLVLALLSGLAFSNTPLAV
                     ICAILCAVLLAFGIYMYACHECFAFGKGDMMAKVHAHLVAHLNWDGHGTLLDIGCGAA
                     PLTVRCAKAFPDAALIGMDYWGAQWNYAKEQCEKNAAIEGVASRIHFEKGDAAHLNYA
                     DETFDAAVSNFVFHEVHSARDKRDVVREALRVVKRGGAFSFQDLFAQKSLYGDMDAFV
                     EELKKEGISEIHYIGNLEKTLDFVPAYVRTPWMISGMGILYGKK"
     gene            complement(4320..4757)
                     /locus_tag="ACZO9R_00790"
     CDS             complement(4320..4757)
                     /locus_tag="ACZO9R_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911641.1"
                     /translation="MGTKQYERIFQILGAVLLAAVVVGVIAVCFGNGARSGVKREIGA
                     SDYYSQEEISAAMDTAQEYFDDHFPGCVMTAFRYDEESVREAEPEWADQYQADQAIVL
                     LSDFQTDKHGGDGSLNPNEIYQDWQWVLVRSGNGDWTLKTCGY"
     gene            complement(4900..5889)
                     /locus_tag="ACZO9R_00795"
     CDS             complement(4900..5889)
                     /locus_tag="ACZO9R_00795"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005427434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR0911642.1"
                     /translation="MITLNDYLYSGDTVLEILKKYTEDLRQSAAQTGNEVDVRHCEFL
                     SQIMELLEHNDFLTAQSQKIREFYKYMAREYPYLSFTFKGRIKSLIRAEAKFNGYVVE
                     YIYDYYTKNGTYPPVPELQERLNCFRDLIAYRIVISMPKCHLKNPDDGEREEIRYLYE
                     TANVLKGFLEERGFTAESAGGVKESDSPLLSDAVRPYYRDYIAHVDPDGYRSLHITFF
                     DNSAKCYMEMQLRTKRMDDIAEIGPANHLGYEKKQESDRARRDAIPEGECVYFDEAYE
                     RCRRLHELELAKLDVNMFAAIDNSLINDGCGLYRGRLILPYEHLSRFQNDLID"
     gene            complement(5936..6541)
                     /locus_tag="ACZO9R_00800"
     CDS             complement(5936..6541)
                     /locus_tag="ACZO9R_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0911643.1"
                     /translation="MDQIKTGKFIADERKKKGYTQRQLAEQLGISDKTISKWERGNGF
                     PEISLLLPLCNELDLSVNELLTGERISETEYREKAEENMVNLVKEAQESKKKIILSGM
                     VSALVIIAATPMFVVAGAFPMEDWMRIALIAVGIVVIVIGIAIACILDRDAGAYECPE
                     CKTRFVPEMGAYVMGPHTLTRRKLVCPHCGAHRYCKKVLTR"
BASE COUNT         1679 a         1985 c         1501 g         1932 t
ORIGIN      
        1 ttgcataaga aaaaagcctg aataatcagg cctatttctt atgcaaaaaa atcaaaaatt
       61 gtgttgacat ctcttaacag gtctaatggc accgcttcac agacaatata aaaccgcgcg
      121 aaccgaaact tgttggcatc tgctattgaa aatagggcag atgccaattc agacatcggc
      181 ttcgcgcggt tatgttttgt tcgacataaa ggtggcattt atgccagctg ttgtctggtg
      241 ccaataaggg acacacaaaa gtcctaaaat tgtctgttgg cttgcggaca cagtccgtag
      301 agtgctccaa acttggaaag acacggatca tcgcagatgc agtcaccggc tggttagaaa
      361 tgccgcattt tttgaacaga atgttccata ggcattccag ccgaaatcgt aggggtatgc
      421 ttctcatagt ataacttatc actcaaagct gcttacctgc ttcgtgcggc agcttctttc
      481 agatggctcc gtcccgcgtt ctatacacat gcaaatgccg cggattcttc cttgagattc
      541 tttgccacgg tactgttctc ctccatctgt ccgtttacag tggacatgga ctgtaccagg
      601 gaatcaatgt tcgtggctgc atcagtcacg ccgtttgcgg actcttcgac cgcacggctg
      661 atgccctcaa ttgcctctgt catctccatc attgtcgcca gtatatcctg cgcctcgccc
      721 gcatactctg ccatcgtgtt gtcgatgtgg gtcgcatcat cattgtactg ctgaccaccc
      781 tgcacaaatg cacggtaatc cggcagaatg ttctcgttga tataaccaac aatcttctcc
      841 gaagaaacaa ccagcccctg tactgcctca attacctgct cattgatcgt ctggatattg
      901 ttcgctgtct cacgcgaaga atctgcgagc tgtctgatct catctgcaac aactgcaaat
      961 ccctttccgg cttcaccggc acgcgctgcc tcaatcgatg cattcagcgc aagcaggttt
     1021 gtctggctgg agatactcag aatatccgcc gtcagctgtg cgaccttctc cacactcttg
     1081 gaattctcga gcgcctggtt catctccgtc gtaatctcgc cgatcatctc gttggtgttg
     1141 ttcatgttct ctgtcgccgt gtgctcaagc tccgtcgcac gcttcttcat atcctgcgca
     1201 tattgtgaca taaccttggt ctgatctgcc atcttctgta ctttgccttc cgcagcggtc
     1261 gtattctcgc tgacgctgtt cgtcgttgcg gatacctcct ccatcgttgc agaaagctct
     1321 tccatgatcg cggaaatatc attggcattg tcactggacg ctgcaacgct gcttgctaca
     1381 tttccgacaa ctccgtcaag gacattggaa ttgctgatga tgttggacat gatattctgc
     1441 agcgtttcga tgaaatggtt gataccgatc gatgactgtc cgatctcatc catcgacttt
     1501 accgtaacgc gctttgtgag gtcaccgtgt ccacctttga tctcatcaat aatctctgtc
     1561 agttcactcg tctgctttct gagcggcttt acaaccgtct tgataatcac aacaaccacc
     1621 atggcaaatg ccacaaaaga aattgccagc aggatcagcg atagtatcat attctggttg
     1681 tacagatctt tctgttcctg aatattctgc ttcaccaatt catcgttgcg agatatgagc
     1741 gtatccatat tgaccgcaat cgtatctgac cactccgtca tcacagaatt tgcctttgcc
     1801 accgctgctg cagaatcacc ggccatcgcc gtctgcatca gttccacggt tgtcccctgc
     1861 gcctctgtga gcagatcaaa tgtctcattc ataagctgga tatcatctgc cgtaaaatag
     1921 tgatccatcg ccagaagctc cttttcatag ctgtccacgt tcgactggta ttccgtcagc
     1981 tgggatgcca catactcata cagatccttg ctcggctctg cgcagagcgc aagcgtaagc
     2041 ttctgcgtgt tggcaaaagc aagattgatc tcatccatac agattgtcgc acgtacaccc
     2101 tctccggaga tctgattgct ttccttctga acggcggtca tggaataaag actcacgcct
     2161 gcaatcataa tcgcgatgca cccaagaacc gcaaccggta taatgagttt ggcaataacc
     2221 gatgattttt tcttcatcct ttcgtatcct ccattaaaat tgtctatagc cccattttat
     2281 catgatatgc ggacatattc tattcatttt tttaacgaaa tctatacatt aaaaaaaatc
     2341 ctcgtatatc ttgacattca agttagtttg tactaatata acagttgtta tggaagaaga
     2401 ccgaaaaaaa ttaaccgaaa agaaattact ttcctgcggg aaacgggaat ttctggaaaa
     2461 gggatatgcg aaggcgaacc tccgctcaat ctgcgaagct tccggcgtga caaccggcgc
     2521 cttttacttt tcttttgcga gcaaggaagc ggcgctgctt cgcgccatct tagatccctt
     2581 catcgcagat tccagagagc tcctttccgg gcttgcccgg atggaggagg aacatcccga
     2641 gactgcagac gacaatgaga tccagatcat ccggtatctc tccgcacacc gtgaggaatg
     2701 tattatcctg atggagaaat ccgccggcag ctgctatgaa cattttcatg aggaagcagc
     2761 cgcgatgatg caggaatctt tccgccgtta ttacagaaag tatctcggcg ccgaaccgga
     2821 tccggaactg atccggattc ttgcgtccat gcgcctgcac gggtatctgg aattagttca
     2881 tggagactac acaatgaaag aaagactgtt tttagccaga gcgatcggca tccacgccga
     2941 cgccggcacc ctgagtttaa tacaatattt aaaggaagag aatgatgctc accgttaatc
     3001 cggtgggcat tttcttttat aaaacataac aaaaaaaata tttgttatcg aggaggaaaa
     3061 cacaatgaat caaaaaccaa actacggaaa ctgggtacct gaaaaactct tgtatctgac
     3121 cttcggcggc gccgctctct ttctggtgct tgcactgctg tcaggtcttg ctttctcaaa
     3181 cacgcccctc gcggtcatct gcgccattct ctgcgccgtt ctgctcgcct tcggcatcta
     3241 tatgtatgcg tgccacgaat gctttgcctt tggaaaaggc gatatgatgg caaaggttca
     3301 cgcacatctg gtcgcccatc tcaactggga cggacacggc acgcttctcg acatcggctg
     3361 cggcgctgcc ccgctcaccg tccgctgtgc caaggcattc ccggacgcag cgctcatcgg
     3421 catggattac tggggcgcgc agtggaacta cgccaaagag cagtgcgaaa aaaatgcggc
     3481 aatcgagggc gtcgcttccc gcatccattt tgagaaaggc gatgcggcac acctgaacta
     3541 tgccgacgag acgtttgacg ccgccgtcag caacttcgtc ttccatgaag tccactccgc
     3601 cagagacaag cgtgatgtcg tgcgggaggc actgcgcgtc gtaaaacgcg gcggagcttt
     3661 ttctttccag gatctcttcg cccagaaaag cctctacggc gacatggatg cctttgtgga
     3721 agagctgaaa aaggaaggca tttccgagat ccactacatc ggaaatctgg aaaaaacact
     3781 ggattttgtc cccgcctacg tgcgcacacc ctggatgatc agcggcatgg gcattctcta
     3841 cgggaaaaaa taacagttcc ctacagctgc gccagcactt cgccgatgtc gccgtcaatg
     3901 cagatcgagc gtccgtcaag ttccgtgatt cctttttcca ttgtcatccc ggcaagatac
     3961 gcctcctgcc gtgtaaagta cgcacttttt ttgccccggt taccggaaag taacttaatg
     4021 aaatgaatct cgcttgcgag attctccgcg cgctcgcggt tcgcaaagcg atattttcct
     4081 taccgtgagc tgcgcatcct cgcggtgcgt tttttatttc ataggaaggt tctactttca
     4141 cacataaatg tatcacgata tttcctatga aacaaaaaag aatcctgctt tgcaggattc
     4201 tccgcctgcg gcgggttgct ctagcgacat aattccactc cgacgcagtg cgatcctgcc
     4261 cggagggctt tttcattcat aggatgtaat ttcctatgaa taaaaaaaag acatgcaggt
     4321 taatacccgc atgtcttcaa tgtccagtca ccgtttccgg agcgcactag cacccactgc
     4381 cagtcctgat aaatttcgtt tggattgagg cttccgtctc cgccgtgctt gtccgtctga
     4441 aaatctgaca gaagaacaat tgcctgatct gcctgatact gatctgccca ttccggctcc
     4501 gcttcccgca cgctctcctc atcgtaccgg aaagctgtca tcacacatcc cggaaaatga
     4561 tcgtcgaaat actcctgcgc ggtatccatc gccgcactga tctcctcctg cgaataatag
     4621 tcggacgctc cgatctctct cttgactccg ctcctcgctc cgttgccaaa acatacagcg
     4681 atcactccca caaccactgc ggcgagcagt acagcaccca gaatctgaaa gatcctctca
     4741 tactgttttg tccccatgtt ctttcccctt attcctgtgt tctctgatag aatgtgtaca
     4801 cattcctatc tttctatcat actctgcctg gctccattcg tcaatcatcc gccacagaat
     4861 ctttcacata aatacagaaa aagcggccgg tgccttctcc tagtcaatca gatcattctg
     4921 aaaacgcgac aggtgctcat acggaagaat cagccggcca cggtacagcc cgcacccgtc
     4981 attgatgagg ctgttgtcga ttgccgcaaa catattgaca tctagctttg caagctcgag
     5041 ttcgtgcagt ctccggcagc gctcgtaggc ttcatcaaag tagacacatt ccccctcagg
     5101 gatggcatcc cttctcgccc ggtcgctctc ctgctttttc tcatacccca gatgattggc
     5161 gggtccgatc tctgcaatat cgtccatccg cttcgtccga agctgcatct ccatgtagca
     5221 cttcgcgctg ttatcaaaga acgtaatatg cagggagcgg tagccgtccg ggtccacatg
     5281 cgcgatataa tcgcggtaat acgggcgcac cgcatccgaa agcagtgggg agtcgctctc
     5341 cttcacacct ccggcggact ccgccgtaaa cccgcgctcc tccagaaatc ccttaagcac
     5401 attggcggtc tcatacagat atcggatctc ctcccgctct ccgtcgtctg gattcttaag
     5461 atggcacttc ggcatggaga tgacaatccg gtacgcgatc agatcccgga agcagttcaa
     5521 gcgttcctgc aattccggca ccggcggata cgttccgttc ttcgtatagt agtcatagat
     5581 gtactccacc acataaccgt tgaacttcgc ctccgcacgg atcaaagact tgatccgccc
     5641 tttgaatgta aaggacagat aaggatattc ccgcgccata tatttataga attcccggat
     5701 cttctgcgac tgcgccgtca ggaaatcatt gtgctcaagc aattccataa tctgcgacag
     5761 aaattcacag tgcctgacat ccacttcatt cccggtctgc gctgcggact gccgcaggtc
     5821 ttcggtgtat tttttcaaaa tttctaagac ggtgtctccg gagtagagat aatcgtttaa
     5881 tgtaatcata ggcgtacctt ctttgggttt ctacctatat tttcagatat attttttatc
     5941 tcgtcaacac ctttttacag tatcggtgcg ctccgcaatg cgggcatacc agcttcctcc
     6001 tagtcagcgt gtgcggtccc ataacataag cacccatctc aggcacaaac cgcgtcttac
     6061 actccgggca ctcataagct cctgcatcac gatcaagaat gcatgcaatc gcaatgccga
     6121 tcacaatgac aactatgccg actgcaatca gcgcaatccg catccagtcc tccatcggaa
     6181 atgctcccgc taccacaaac attggcgtag ctgcaataat cacgagcgcc gataccattc
     6241 cggataaaat aattttcttc ttgctttcct gtgcttcttt cactaaattc accatattct
     6301 cctccgcttt ctcccgatac tctgtctcgg aaatccgttc tcctgtcagc aattcgttta
     6361 cagatagatc cagttcgttg cataacggca gcagcagtga aatctccggg aaaccatttc
     6421 cgcgctccca ttttgagatc gtcttatcac tgattcccag ctgctctgcc agctgtctct
     6481 gcgtgtaacc ctttttcttt ctctcatctg caataaattt tcctgtctta atctggtcca
     6541 tgtcttcctc ctttctggtc tcattatgtc cccgctccca cggctttcac acacacgcag
     6601 cgtagaatat ggtgaatttg tgcggattct acgctccgtc gagtgcctct tgccctcggt
     6661 ctctcggttt catgaccggt tatcctcaac tcaggcaact acagtttcct tctccggcaa
     6721 ccgccgatcc aggtcccggc agctacagtt tcccacttta atgcaaaaaa ggctcccaag
     6781 gaaaaccctg ggagccttaa atttactgaa taatatttaa ttactcgatg attgtagcaa
     6841 cacggcctga acctacagtt ctaccaccct cacgttattt gaataccgta tttctgcata
     6901 ttttctatgc ttttaggtgg caaaatctgc agaaaactgc tgtttttgca tactgtattt
     6961 ttatttgcct aagttcttgg caccgttttg gcaccgtagg tacttcatct tcctttacta
     7021 atacaaactt gaattatcgt agtgcatctc ttaacttcca cagaccactt tggatatttg
     7081 ctcctgcacc ctcgata
//
LOCUS       DBMDQJ010000021         7086 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4435_length_7086_cov_1.649392, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000021 DBMDQJ010000000
VERSION     DBMDQJ010000021.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 7086)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 7086)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7086
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4435_length_7086_cov_1.649392"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..759)
                     /locus_tag="ACZO9R_00805"
     CDS             complement(<1..759)
                     /locus_tag="ACZO9R_00805"
                     /inference="COORDINATES: protein motif:HMM:NF019874.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR0911644.1"
                     /translation="MIILALITLIGTLAIGILSVNRILIMERGSESVLRASIEEDYDE
                     NIKNQVDNAISMLDAVYAGYENGDYSYEEAETRGADLLRELRYGNGGYFWADTYDGEN
                     VVLLGSATEGTNRMETKDADGYQMVKEIIRVGQEPDGGYTDYVFPKEGETESSPKRSY
                     SRAFEPFGWVVGTGNYIDYIDETVEKETQAMRENVKTALIAIIGTGSLLIVIVMAVCL
                     YMAFSLSRSFQVAQIQKVTEEVTSSVAQLSSDAEQ"
     gene            1079..2575
                     /gene="glpK"
                     /locus_tag="ACZO9R_00810"
     CDS             1079..2575
                     /gene="glpK"
                     /locus_tag="ACZO9R_00810"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019163288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR0911645.1"
                     /translation="MAKYIMALDAGTTSNRCILFNEKGEMCSVAQKEFTQYFPKPGWV
                     EHDAEEIWSTQLEVAKEAMANVGATAADIGAIGITNQRETTIVWDKNTGEPIHHAIVW
                     QCRRTSEYCDSLKAKGLVDTFRQKTGLVIDAYFSGTKLKWLLDNVEGARERAERGELL
                     FGTVETWLIWKLTQGQVHVTDYSNASRTLMFNINTLEWDEDILKELNIPKCMLPEPKP
                     SSCVYGEANPVFFGGPIPIAGAAGDQQAALFGQTCFTAGEAKNTYGTGCFLLMNTGEK
                     PVFSKNGLVTTIAWGLDGKVNYALEGSIFVAGASIQWLRDEMRFIDSSPDSEYMARKV
                     KDTNGCYVVPAFTGLGAPYWDQYARGTIVGITRGVNKYHIIRATLESLAYQVNDVLAA
                     MKADSGIDLAALKVDGGASANNLLMQMQADISNAPVNRPMCVETTAMGAAYLAGLAVG
                     YWASKEDVLQNWAIDRTFTPEITDEERNKKVRMWKKAVTYSFNWAKED"
     gene            2610..3320
                     /locus_tag="ACZO9R_00815"
     CDS             2610..3320
                     /locus_tag="ACZO9R_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR0911646.1"
                     /translation="MTPYIAEFVGTMILILLGDGVVANVNLNKSGMKGAGAVQITLAW
                     GLAVLVPAFIFGAASGASFNPALTIALAADGSFAWSMVPGYIVAQLAGAFCGACLVYL
                     LFKDQFDATEDAATKRGVFCTAPAVRNIPRNILSEAVGTFVLVFAIKGLAQVPDLADG
                     TGKFLVFAIIVSVGMSLGGLTGYAINPARDLGPRLAHAVLPIQGKGSSDFGYGLVVPI
                     VGPILGALAAVLVYGLIF"
     gene            3413..4849
                     /locus_tag="ACZO9R_00820"
     CDS             3413..4849
                     /locus_tag="ACZO9R_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0911647.1"
                     /translation="MYDVVIIGAGVSGSACARELSRYNLSICVVEKEEDVCCGTSKAN
                     SAIVHSGIDCKPGTLMSEMNVRGNELMEPLSKELDFEFRRNGSLIVCFSEEDRPKLEA
                     LYEQGIANKIPDIRILDAHEVHEMEPNLSEEVVAAIYCPTGGIVCPFGLNIALAENAA
                     TNGVEFRFNTEVTDICRKDDGYQLSTNRGYILTRCVVNAAGVYADVFHNMVSSKKIHI
                     TPRRGEYCLLDKTTGGLVDKTIFQLPGKYGKGILVTPTVHGNTLIGPTADDHDDKEAT
                     NTTRQGLDHVCSAARRSVPSVPVRQVITSFAGNRAHEDNHEFIIEELADAPHFVDCSG
                     IESPGLTSSPAIGERVAQIVSGLLHAQKNPAWNGSRKGVLNPKTLSEEDYAALIRENP
                     AYGNIICRCEMITEGEILDAIHRPLGARSLDGVKRRTRAGMGRCQAGFCSPRTMEILA
                     RELGISQLEITKNGGSSNMVVGLSKDRT"
     gene            4869..6155
                     /locus_tag="ACZO9R_00825"
     CDS             4869..6155
                     /locus_tag="ACZO9R_00825"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0911648.1"
                     /translation="MTSYDIVIVGGGPAGLAAAVSAKNNGIDRILILERDRELGGILN
                     QCIHNGFGLHTFKEELTGPEYARRFINQALDLNIEYRLNTMVMDISLAEGSSNKIVTA
                     MNRTDGLFHINAKTVILAMGCRERSRGALNIPGYRPAGIYSAGTAQRLVNIEGFMPGR
                     KVVILGSGDIGLIMARRMTLEGAEVKVVAELMPYSGGLKRNIVQCLDDFGIPLKLSHT
                     VVDIRGKERVEGITLAQVDEHNKPIPGTEEDYECDTLLLSCGLIPENEISRSMGVELN
                     PVTSGPSVNESLETNIPGVFACGNVLHVHDLVDYVSEEAGVAGKNAAKYIKCGGDHAD
                     SGKEIPIVATDGVRYTVPKTLHVANMDDELTVRFRVGGVYKNCYISTYLNDQRIICRK
                     RPVMAPGEMEQVKLQKSVLEAMSDLDHITIKIEEEA"
     gene            6159..6521
                     /locus_tag="ACZO9R_00830"
     CDS             6159..6521
                     /locus_tag="ACZO9R_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1667 domain-containing protein"
                     /protein_id="HIR0911649.1"
                     /translation="METRNLTCIGCPLGCAITVTMDGGNVVSITGNTCKRGSDYAAKE
                     VTHPTRIVTSTVCVRGGSIPMVSVKTAQDIPKEKIFEIMKYINALTVTAPVHIGDVLL
                     KDIAGTGVDLIATKEVLS"
     gene            complement(6606..>7086)
                     /locus_tag="ACZO9R_00835"
     CDS             complement(6606..>7086)
                     /locus_tag="ACZO9R_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911650.1"
                     /translation="EATGMSRQSFYYHFKDIYDVLEWIGCNDFKDQLQGQYDSMEKWA
                     CDLMEVLQRERSFYEKLANELAWPMIVRGVRMALDAQVRRLLLGENPGMFEKHPEELE
                     AVTSFLSTSICYYMIDFVYYRKTLSGEQVKRDVQFMMRAIAKPDAGLAVLLPRTAVL"
BASE COUNT         1772 a         1961 c         1737 g         1616 t
ORIGIN      
        1 ctgctccgca tcagaagaaa gctgtgccac agaagatgta acttcctccg tcaccttctg
       61 tatctgggca acctgaaaag atctgctcag tgaaaacgcc atatacagac agaccgccat
      121 gacaatcaca atcagcagac tgcccgttcc gataatcgcg atcagtgccg tttttacatt
      181 ctcccgcatt gcctgcgttt ctttctcaac agtctcatcg atatagtcaa tatagtttcc
      241 cgttccgaca acccagccaa acggctcaaa tgccctgctg taggatctct tcggagaaga
      301 ttccgtctca ccttctttcg ggaatacata atccgtatat ccgccgtccg gttcctgccc
      361 gacgcggata atctctttta ccatctggta accatccgca tccttcgtct ccatgcggtt
      421 cgtcccttcc gtcgcgcttc cgagcagaac cacattctct ccatcatagg tatctgccca
      481 gaagtatccg ccgttcccgt acctgagttc ccgcagcaga tctgcaccgc gtgtttccgc
      541 ttcctcatag gaataatcac cattctcata gcctgcatac accgcatcca gcatggaaat
      601 cgcattatct acctgattct taatattctc atcgtaatct tcttcgatac ttgcgcggag
      661 aacactctct gaacccctct ccataatcag aatccggttt actgacagaa ttccgatcgc
      721 cagtgttccg atcagtgtga tcagtgccag aataatcatc ttctaacgta cactcacatt
      781 tttcatacct atccgactcc ttcctgtgct tacttctttt attatcgtat attccccact
      841 aatcgtctat atttgttaat aatttagcaa tctattcatg ttttttgtca atattgcata
      901 tatgtaaaaa cgcaggactc ctcttttaca atcacagaag tctgtctaaa gacattcacc
      961 cgcaacgcag ttacactaaa gccacaaacg gtgagcggca catcagaccg ccactcatac
     1021 aaccagaggg gaaccgggtg gggccggttt ccacactaag aaaatggagg gactttacat
     1081 ggcaaagtac atcatggcac tcgatgccgg aaccaccagc aaccgctgca tacttttcaa
     1141 tgagaagggt gaaatgtgca gcgttgccca gaaagaattt acacagtatt tcccgaagcc
     1201 gggctgggta gagcacgacg cggaggagat ctggtccaca cagcttgaag ttgccaagga
     1261 ggcaatggca aatgtgggcg cgactgcagc cgatatcggg gcaatcggaa ttaccaatca
     1321 gcgcgagacc accatcgtat gggataagaa caccggcgaa ccgattcatc acgcgatcgt
     1381 atggcagtgc cgccgtactt ccgaatactg cgattcctta aaagccaagg gactggttga
     1441 taccttccgc cagaagacag gactggtgat cgacgcctac ttctccggaa cgaaattaaa
     1501 atggctgctc gacaacgttg aaggcgccag agagagagcc gagagaggcg agctgctctt
     1561 cggaaccgtg gagacatggc tgatctggaa actgacacag ggtcaggttc acgtaactga
     1621 ttactccaac gcttccagaa ccttgatgtt caacatcaat accctggagt gggatgaaga
     1681 tattttaaaa gaactgaata ttccaaaatg tatgctgccg gaaccgaagc catccagctg
     1741 cgtgtacggc gaagcaaacc cggtattctt cggaggcccg attccgattg ccggtgctgc
     1801 cggtgaccaa caggccgctc tgttcggtca gacctgtttc accgccggtg aggcaaaaaa
     1861 tacttacgga accggctgtt tcttacttat gaacaccggc gaaaaacctg tattctccaa
     1921 gaacggactg gttaccacga tcgcatgggg tctcgacgga aaagtaaact acgcactcga
     1981 gggttccatc ttcgttgcag gtgcctccat ccagtggctt cgcgatgaga tgcgcttcat
     2041 cgattcctct ccggattccg aatacatggc aagaaaagtc aaagacacca acggctgcta
     2101 cgtcgtgcct gctttcaccg gtcttggtgc cccgtactgg gatcaatacg cacgtggaac
     2161 tatcgtaggc attacccgcg gtgtgaataa ataccatatt atccgtgcaa ccttagagtc
     2221 tctcgcttac caggttaatg atgtattggc tgcaatgaaa gcagattccg gtattgatct
     2281 tgcagcatta aaggtagacg gcggagcaag cgcaaacaat ctgctcatgc agatgcaggc
     2341 ggacatcagc aatgctccgg tcaaccgtcc gatgtgcgtg gagaccacag ccatgggtgc
     2401 tgcctatctc gccggactgg cagtcggcta ctgggcaagc aaagaggatg ttcttcagaa
     2461 ctgggcaatc gaccgcacct tcacaccgga gatcaccgac gaggagcgta acaagaaagt
     2521 ccgcatgtgg aagaaagccg ttacttattc gttcaactgg gcgaaggaag actaacacac
     2581 aatttcatat ctaagagggg gtatttatta tgacaccata tattgcagag tttgtcggca
     2641 ccatgatcct catcctgtta ggtgacggtg ttgttgccaa tgtcaacctg aacaaatccg
     2701 gcatgaaagg tgccggtgca gtccagatca cacttgcctg gggtcttgcc gtactggttc
     2761 ccgcctttat cttcggcgcg gcatccggcg caagctttaa ccctgcattg accatcgcgc
     2821 ttgctgctga cggcagtttt gcatggagca tggtaccggg atatattgtg gcacagctgg
     2881 ctggtgcttt ctgcggcgca tgccttgtat atctgctgtt caaagatcag tttgacgcaa
     2941 cagaagacgc ggcaacgaag cgcggcgtgt tctgtacggc acctgcagtg cgcaacatcc
     3001 cgcgcaatat cttaagcgag gctgtcggca cttttgtact cgtatttgcc atcaagggtc
     3061 tggcacaggt tccggatctg gcggacggca ccggaaaatt tctcgtattc gccattatcg
     3121 tatctgtcgg tatgtctctg ggcggtctga ccggatatgc aatcaatccg gcaagagatc
     3181 tgggtcctcg tcttgcacat gcagtgcttc cgattcaggg aaaaggttcc tctgatttcg
     3241 gatacggact cgttgttccg atcgtcggac cgatcctcgg cgcattggct gccgtactgg
     3301 tatatggact tattttttaa aactatagat tgaatgtcag agaagagagc caggcagaaa
     3361 gcacatcaga aatgatgtct gatctgcatg gctcttttaa ggaggaaaaa ctatgtatga
     3421 tgtagtgatc attggtgccg gcgtctctgg aagcgcctgt gcacgtgaac tttccagata
     3481 caatctctcc atttgtgttg tggaaaaaga ggaggacgtc tgctgcggca cttccaaagc
     3541 caacagcgcc atcgtccact ccggtatcga ctgtaaaccc ggaaccctga tgtcagagat
     3601 gaacgtccgg ggaaatgaac tgatggaacc gctctccaaa gagctggatt ttgaatttcg
     3661 cagaaacggt tccctgatcg tctgcttctc cgaggaggac cgcccgaagc tggaagccct
     3721 ctacgagcag ggcattgcca acaaaatccc ggatatccgg atcttagatg cacacgaagt
     3781 tcatgagatg gagccgaacc tctccgagga agtcgtcgct gccatctact gtccgaccgg
     3841 cggcattgtc tgcccgttcg gtttgaacat tgcactcgca gaaaatgctg ccacaaacgg
     3901 cgtcgaattc cgttttaaca cagaagtgac agacatctgc cgcaaggacg acggctatca
     3961 actctccacc aaccgcggtt acatcctgac ccgctgcgtg gtaaacgctg ccggtgtgta
     4021 tgcagatgtt tttcacaaca tggtcagcag caagaagatc cacattaccc cgcgccgcgg
     4081 cgaatactgc ctgctcgaca aaacaaccgg cggtctggtc gacaagacca tcttccagct
     4141 tccgggcaaa tacggcaagg gcattcttgt aaccccgacg gttcacggca atacgctgat
     4201 cggccctacc gcagacgatc acgacgataa ggaggcaacc aacaccaccc gccagggact
     4261 tgaccatgtc tgcagcgcag caagacgctc cgtgccttcc gttccggtcc gtcaggtaat
     4321 cacgtccttc gccggaaacc gcgcccatga ggacaaccat gaattcatta ttgaagagtt
     4381 ggcggatgct ccacactttg tcgactgctc cggcatcgaa tctccgggac tcaccagttc
     4441 cccggcgatc ggcgagcgcg tagcgcagat tgtatccgga ctgcttcacg cacagaaaaa
     4501 cccggcatgg aacggcagcc ggaagggcgt tctcaatccg aaaacactct ccgaggaaga
     4561 ttatgcggcg cttatccgcg aaaatccagc ttacggaaat attatctgcc gctgtgagat
     4621 gattaccgag ggagaaatct tagacgccat tcaccgtccg cttggcgcac ggtcattaga
     4681 cggcgtcaag cgccgtaccc gtgcaggcat gggacgctgc caggcaggtt tctgctcgcc
     4741 gcgcaccatg gaaatcctgg cgcgtgagct tggtatctcc cagcttgaaa tcacgaaaaa
     4801 cggcggctca tccaacatgg tcgtcggtct gagcaaagac agaacctgaa ggaaaaagga
     4861 ggattattat gacttcatat gatattgtga tcgtcggcgg aggtcccgcc ggtcttgcgg
     4921 cagccgtttc cgccaaaaat aacggcatcg accggatcct gattctcgag cgcgaccgcg
     4981 aactcggcgg catcttaaat cagtgcatcc acaacggttt cggtcttcac acctttaagg
     5041 aggagctgac cggcccggaa tacgccagac gctttatcaa tcaggctctc gatctgaaca
     5101 tcgaataccg tctgaacacg atggtgatgg atatttctct ggcagaaggc tcttccaata
     5161 agatcgtgac ggctatgaac cgcaccgacg ggctgttcca cattaacgca aagaccgtga
     5221 ttcttgccat gggctgccgc gaacgttccc gcggagcttt aaatatcccg ggctaccgtc
     5281 ctgcaggcat ctactctgcc ggaaccgcgc aacgcctggt gaacatcgag ggctttatgc
     5341 cgggacgcaa ggttgtgatc ctcggctccg gagacatcgg cctcatcatg gcacgccgta
     5401 tgaccttaga gggcgccgaa gtcaaagtcg tcgccgagct gatgccttat tccggcggtc
     5461 tgaaacgaaa cattgtacag tgtctggacg atttcggtat tccgttaaag ctgagccata
     5521 ctgtcgttga catccgcggc aaagagcgcg tggagggaat cacactggca caggtggatg
     5581 aacacaacaa gccgattccc ggcacggagg aagactacga atgcgacaca ctgctgctct
     5641 cctgcggtct gattccggaa aatgagattt cccgctctat gggcgtcgag ctaaatccgg
     5701 tcacctccgg tccttctgtc aacgaaagcc ttgagacaaa tattccgggc gttttcgcct
     5761 gcggtaatgt tcttcacgtt catgatctcg tggactatgt atccgaggaa gccggcgttg
     5821 ccggaaagaa tgccgcaaaa tatatcaagt gcgggggcga tcatgctgat tccggcaaag
     5881 agatccccat cgtcgcaacc gacggcgtcc gctataccgt tccaaaaacg ctgcatgtcg
     5941 ccaatatgga cgacgagctg accgtgcgtt tccgcgtcgg cggtgtatac aagaactgtt
     6001 atatttccac ctacttaaac gaccagcgga tcatctgccg caagcgtcct gtcatggctc
     6061 ccggcgagat ggagcaggta aaactgcaga agtccgttct ggaagcaatg agtgacctgg
     6121 atcacattac catcaaaata gaagaggagg cgtaacacat ggaaacaaga aatcttacat
     6181 gcatcggctg cccgctgggc tgtgccatca cagttaccat ggatggcgga aatgttgtct
     6241 ccatcaccgg aaatacctgc aagcgcggct ctgactatgc cgcaaaggaa gtcacacatc
     6301 cgacgcgcat cgtcacaagc accgtctgtg tccgcggcgg ctccatcccg atggtatccg
     6361 tcaagaccgc acaggatatt cccaaagaaa agatcttcga gatcatgaaa tatatcaacg
     6421 cgctgacggt gacggctccg gtacacatcg gagatgtcct gctgaaagat attgcaggaa
     6481 ccggcgtcga cctgatcgcc accaaagaag ttctctctta gaaccgcaga tgcagctgca
     6541 aaagtaaaaa agtgccggta agccagacca gcttaccggt actttttaat tttgtcagaa
     6601 tagcattaca atactgcggt gcgcgggagc aatacggcaa gccccgcgtc cggttttgca
     6661 atcgcccgca tcataaactg cacatcccgc tttacctgtt cccccgataa cgtttttctg
     6721 taatatacaa aatcaatcat atagtagcag atggatgtgg aaagaaagct cgtcaccgcc
     6781 tccagttcct cgggatgctt ttcaaacatg cccggattct cgccaagcag cagacggcgc
     6841 acctgcgcat ccaacgccat gcgtacacca cgcacgatca tcggccacgc gagctcgttc
     6901 gccagcttct cataaaagct gcgctccctc tgcagcacct ccatcagatc gcatgcccac
     6961 ttttccatac tgtcatactg tccctgcagc tgatccttaa agtcattaca gccgatccac
     7021 tccagcacat cataaatatc cttaaaatga taataaaaac tctggcggct catccccgtt
     7081 gcctcc
//
LOCUS       DBMDQJ010000022         6994 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4492_length_6994_cov_1.527782, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000022 DBMDQJ010000000
VERSION     DBMDQJ010000022.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4492_length_6994_cov_1.527782"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /gene="dctP"
                     /locus_tag="ACZO9R_00840"
     CDS             complement(<1..392)
                     /gene="dctP"
                     /locus_tag="ACZO9R_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR0911651.1"
                     /translation="MRRICAAAVVAGMTILLTLSAWKNHTYLSKTEVEPEYVFSYAEN
                     QPEDYPTTLGAKYFAELVEERTNGRIRILVQPVGVLGSENKVIKQMQYGGIDFARVSL
                     AQLAEYIPSLNVLQMPYLYTDSDHMWRVL"
     gene            complement(389..1207)
                     /locus_tag="ACZO9R_00845"
     CDS             complement(389..1207)
                     /locus_tag="ACZO9R_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0911652.1"
                     /translation="MKRVLIADDEPIERMIVSRTVEKYFSEELTVVSAANGREAVEQF
                     FAGDCQIALLDIEMPGMNGLEAAEQIRKRDKNCSIIFLTAFDEFNYARRAIAVRAMEY
                     LLKPVEDQELVAVLEEAVRIAEEQETDREAGEKKSAEQISAERDCHEDTADTERSRIR
                     LQAVAGNIQAFIEHNYMDDISLQTVAEAMNYSDAYFCKIFKQCFDKNFIVYLSEYRVA
                     RAKELLGDVLINVKDVSQKVGYRDSNYFAKVFKRIAGVTPTEYRMQVLKEAGER"
     gene            complement(1204..2700)
                     /locus_tag="ACZO9R_00850"
     CDS             complement(1204..2700)
                     /locus_tag="ACZO9R_00850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911653.1"
                     /translation="MRKRIKQFWQALTIKKKIATFTGTVFLIIAVSVLFNVWVVKFSL
                     IDFNRILQDNAAVSELAQALEEESIQFETYIKGNREERESLDIAIERTGRAVEKLPFR
                     YSEIGEQRYAKTWSIKSCYEVYCQKRDAMLAMGENAPDYIKRLYEVYEMQDYLEEYAA
                     TLMNETIEDGDGLYHKKVPMLICMPIVVVVSGIILVTGMMELAALMNQTIISPVVALV
                     KAAQRIAANDFFSEDVQVQNEDELGELVHAFNKMKYATGEYIMALEEKRKTLDLLHEE
                     ELEKLETEKRLEMIKLELLKSQINPHFLFNTLNVISGMARLEEAQTTEKMILALSSLF
                     RYNLKTPEQFVLLAKELNVAADYMYLQQMRFGERIRYELDCKARQELVMVPAFTFQPL
                     VENAIIHGIAPKEEGGSIRIVVRQKENRLRIAIGDDGIGMTEEELQQLKNRLKTADAG
                     HGGIGLGNVYRRITAMYPDGDFEICSKKDAGTVIVIDIPCREETGDMT"
     gene            complement(2795..3268)
                     /locus_tag="ACZO9R_00855"
     CDS             complement(2795..3268)
                     /locus_tag="ACZO9R_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR0911654.1"
                     /translation="MEYQYKCRITAWDFFRLTMRQTYRSMAGMCNLVFTVAMIVLAAR
                     FWSRSGEVLQVMMLIGCLLFPVIQPAAIYAKARRQAAAVPQDVRLTFDEKGLLVTTGG
                     EKQHLPWNRLRVTKQRGMVLVLSGAGSGYMLTNRVLGAEREAFLSFAQSKVEQNR"
     gene            complement(3305..>3716)
                     /locus_tag="ACZO9R_00860"
     CDS             complement(3305..>3716)
                     /locus_tag="ACZO9R_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009663286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HIR0911655.1"
                     /translation="CLTTLHVPDLAAKAIIGFSSNPIVIALLVDLILLILGCIMDMAP
                     IILIATPILLPIATSIGIDPVQFGIIMVLNCGIGLLTPPVGSVLFIGSAVSGVKMERV
                     VKATLPFYLCMIVVLLLLTFIPELSIGLPMLLGT"
     assembly_gap    3717..3726
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3727..4591)
                     /locus_tag="ACZO9R_00865"
     CDS             complement(<3727..4591)
                     /locus_tag="ACZO9R_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR0911656.1"
                     /translation="MSVQSMAIAVLLVSFLVMILLRFPIAYAVALSSLLCLLSQGLPL
                     TTICQQMVKGISSFSLMAVPFFITMGVLMGTGGISDKLIALADACVGWMTGGMAMVNI
                     VASYFFGGISGSASADTASIGSIMIPMMVDQGYDDDFSTAVTITSSCEGLLVPPSHNM
                     VIYAMSAGGVSVGSLFLAGYVPGALLAISLMIGSYIISKKRHYPKGDKFSVKRLLKQL
                     GTSFWALAAVVIVVVGVVAGWFTATESAAIAVFYSLIVSVYIYKGLTWKGVWKSLEQC
                     IDTLSIVLILIS"
     gene            complement(4591..5115)
                     /locus_tag="ACZO9R_00870"
     CDS             complement(4591..5115)
                     /locus_tag="ACZO9R_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR0911657.1"
                     /translation="MPKIFQTLDKIKPAYDVAYKFVLFVCKLLLTADIVITSVCVVGR
                     YVSFVPDPAWSEEVVLTLMSYMAVLSAALAIRRNAHIRMTAFDAYLPKNVIKVLDILA
                     DVAVFILAIVMITVGFSYATGIGAKGNYVSMPWLSKFWMYFPIPLAGVAMVVFELEAI
                     YNHIKSIFVKEGTK"
     gene            complement(5241..6359)
                     /locus_tag="ACZO9R_00875"
     CDS             complement(5241..6359)
                     /locus_tag="ACZO9R_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR0911658.1"
                     /translation="MKKKVLSALLCAAMVVSMTACGLKAPEAADTTTSQNTDAAADAT
                     ADATDSAAATEAGDSTGTEPVGPAVTLVYAEVNPLEGTIVGQTATAFKEKVEELSGGS
                     ITIDIQANGVLGAESDVLDTMLGGGGTIDMARISAFALTSYGGEKSSLLSVPFTFVSR
                     DHFWNFATSDLAQEFLLEPHENGTGIRGLFYGEEGFRHFFTVNPIEGMDDLAGMKIRV
                     SNDPIMTGMVEGLGANPTVVAMGELYSALQTGVVDAAEQPIANYQANAFPEVAPNIIL
                     DGHTLGAIQVVITDEAWDSLTPEQQDVLTEAGEYASQYNRQISQETEDKILEELKADG
                     VNVIEVDDITPWQDACKDVIEEATKNNKELYQQIVDMQ"
     assembly_gap    6637..6646
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6728..>6994)
                     /locus_tag="ACZO9R_00880"
     CDS             complement(6728..>6994)
                     /locus_tag="ACZO9R_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR0911659.1"
                     /translation="AMPTIACTKCRYCVEGCPKKIPIPDVFEDYNMTVQFGINETNRG
                     SYRRHTADGGSANVCIRCGKCEGQCPQHLPIRDLLVKVAETFAE"
BASE COUNT         1652 a         2013 c         1470 g         1839 t
ORIGIN      
        1 agcaccctcc acatatggtc ggaatccgta taaagatagg gcatctgcag cacattcagg
       61 ctcggaatat attccgcaag ctgcgccagt gacacccgcg caaaatcaat cccgccgtac
      121 tgcatctgtt tgatgacttt attctctgat ccgagcaccc cgaccggctg caccagaatc
      181 cggattctgc cgtttgtccg ctcctccacg agttctgcaa aatacttggc accgagtgtc
      241 gtcggatagt cctccggctg attttccgca taggaaaaca catattccgg ttccacctct
      301 gtcttggaga gatacgtgtg atttttccag gcggacagtg tgagcagtat cgtcattcct
      361 gccacaaccg ccgccgcgca tatccgtctc atcgctctcc cgcctccttt aatacctgca
      421 tccggtattc cgtcggcgtg acgccggcaa tccgtttgaa tacttttgca aaatagttgg
      481 aatcgcggta gcccaccttc tggctcacat cctttacatt gataaggacg tcacccagca
      541 gttcttttgc cctcgccaca cgatactccg agagatagac aataaaattc ttgtcaaagc
      601 actgcttgaa aatcttgcag aaataggcgt cggaatagtt catggcctct gccaccgtct
      661 gcagcgaaat atcatccata taattgtgtt caataaacgc ctggatattc ccggcaaccg
      721 cctgcagccg gattcttgag cgctccgtat ccgccgtgtc ctcatgacaa tcccgttcgg
      781 cagagatctg ctctgcgctt ttcttctctc cggcttccct gtctgtctcc tgctcctctg
      841 caatccgcac ggcttcctca agcaccgcca ccagttcctg atcctccacc ggctttagca
      901 gatattccat ggcgcggacc gcaatcgcac gcctggcata attaaattca tcaaatgccg
      961 taagaaaaat aatactgcag tttttatccc ttttgcggat ctgctccgcc gcctccagtc
     1021 cgttcattcc cggcatctca atgtccagca gggcgatctg gcagtctccg gcaaaaaact
     1081 gttccaccgc ctcccttccg tttgcagcgc tcacaacggt aagttcctcc gaaaaatatt
     1141 tttcaacggt tctgcttaca atcattctct caatcggttc atcatccgct atcagcactc
     1201 gtttcatgtc atatcccccg tttcctctct gcacggaatg tctatcacga tgactgttcc
     1261 cgcatctttt ttactgcata tctcaaaatc cccgtccgga tacatcgcag tgatccgccg
     1321 gtacacattg ccaaggccaa ttcctccgtg cccggcatcc gccgtcttaa gacgattttt
     1381 taactgctgc agttcttcct cggtcattcc gatcccgtca tccccgattg ctatgcgcag
     1441 ccggttttct ttctgcctga ccacgatacg gatgcttccg ccttcctcct tcggcgcaat
     1501 tccgtgaata atcgcgtttt ccacaagcgg ctggaatgta aatgccggaa ccatgaccag
     1561 ttcctgtctc gctttacagt ccagttcata acggatccgc tccccgaagc gcatctgctg
     1621 cagatacata taatccgcag ccacgttcag ctcctttgcc agcagcacaa actgttccgg
     1681 cgtctttaag ttgtagcgga acagggaact gagcgccagt atcatctttt cggtcgtctg
     1741 cgcctcctca agacgcgcca tcccgctgat gacatttagc gtgttgaaca aaaaatgcgg
     1801 attgatctgg ctttttagca attccagctt tatcatctcc aggcgcttct ccgtctccag
     1861 cttttccagt tcctcctcat gcagaagatc aagagtcttc ctcttttctt ccagcgccat
     1921 aatgtactcg cctgtcgcgt atttcatctt gttgaacgca tgcaccagtt cccccagctc
     1981 gtcctcattc tgcacctgca cgtcctccga gaaaaaatca ttggcggcaa tgcgctgcgc
     2041 cgctttcacg agcgccacca ccggcgagat aatggtctgg ttcatcaatg ccgccagctc
     2101 catcatgccg gttacgagaa taatcccaga taccacaacc acaatcggca tgcatatgag
     2161 catgggcacc tttttatgat agaggccgtc gccgtcctcg atcgtctcat tcatcagcgt
     2221 cgccgcgtat tcctcgagat agtcctgcat ctcgtagacc tcatacaggc gcttgatata
     2281 gtccggcgca ttttccccca tcgcaagcat ggcgtcccgc ttctggcaat agacttcata
     2341 acagctcttg atcgaccagg tctttgcgta gcgctgctct ccaatctcag aatagcggaa
     2401 cggaagcttt tccaccgctc ttccggttcg ttctatcgca atatccagac tttctctctc
     2461 ctcgcgattg ccctttatgt atgtctcaaa ctggatactc tcttcctcga gcgcctgtgc
     2521 cagttcgctc accgctgcgt tatcctgtaa aatccggtta aagtcgatca gggaaaactt
     2581 gacaacccat acattaaaaa gaacagagac tgcaatgatg agaaatactg tcccggtaaa
     2641 cgtcgctatt ttctttttga tcgtaagtgc ctgccagaac tgttttatcc tctttctcac
     2701 gcttgttttt cccatgcctc tctgttctct ctataatgcg gcaaaagcaa agccgtagat
     2761 acacactccg taaatacagc tttgcatatt tgctttatct attctgttcc actttgctct
     2821 gcgcaaagga taaaaacgct tcgcgttccg ctccaagcac acggttggtg agcatataac
     2881 cgcttccggc tcccgagagt accagcacca ttccccgctg ttttgtcacc cggagccggt
     2941 tccacggcag gtgctgtttc tcccctccgg ttgtaacgag cagtcccttt tcatcaaagg
     3001 taagcctgac atcctgcggc actgccgctg cctgccgcct ggctttcgca tagatggccg
     3061 ccggctggat caccggaaac aacaggcatc cgatcagcat cataacctgc aggacctcgc
     3121 ccgaccggct ccagaacctc gccgcgagga caatcatcgc caccgtaaac acgaggttgc
     3181 acattcccgc catcgaccgg taggtctggc gcatcgtaag ccggaagaaa tcccatgccg
     3241 taattctgca cttatattga tattccatat gccgtctcct ttgcgttcgc aaactgatct
     3301 ttctttacgt tccaagtaac atcggaagtc cgatacttaa ttccggtata aaggtcagaa
     3361 gtaataatac aacgatcatg cacagataga acgggagggt tgcttttacc acgcgctcca
     3421 tctttacacc gctgactgca gaaccgatga aaagaacgga tccaaccggc ggtgtcagaa
     3481 ggccgatacc acagtttaat accatgatga taccgaactg taccggatcg ataccgatgg
     3541 aggttgcgat cggaagtaag atcggtgtcg cgatcaggat gatcggtgcc atatccatga
     3601 tacatcctaa aatgagcaga atcaaatcca ctaacagtgc aatcacgatc gggttgctcg
     3661 agaatccgat gattgccttt gcagccagat ccggcacatg aagtgtcgtc aggcagnnnn
     3721 nnnnnntcga aattaagatc aggacgatag acaatgtatc aatgcactgc tccaggctct
     3781 tccagacacc cttccaggta agacccttgt aaatatatac actgacaatc aagctgtaga
     3841 atactgcgat cgcagcggac tctgtcgcgg taaaccatcc ggctacaacg ccgacaacta
     3901 cgattacaac tgctgccaat gcccagaaag aagttccaag ctgttttaac agtctcttga
     3961 cactgaactt atcccccttc ggataatgtc tctttttcga gataatataa gatccgatca
     4021 ttaaggagat cgcaagcagt gcgcccggca catatcctgc aaggaaaagg cttcctaccg
     4081 atacaccgcc ggcggacatt gcatagatta ccatgttatg gcttggtgga accagcagtc
     4141 cctcacagga ggaagtgatc gtaaccgccg tcgaaaaatc atcatcgtat ccctgatcta
     4201 ccatcatcgg gatcatgata cttccgatgg aagccgtatc tgcagatgca gaaccggaaa
     4261 taccgccgaa gaaataggat gcaacgatat ttaccatcgc cattccaccg gtcatccagc
     4321 ctacgcaggc atcggcgagt gcgatcagct tatctgatat acctccggtg cccatcagca
     4381 cgcccatcgt gatgaagaac ggcacagcca tcaaactaaa ggaactgatt ccctttacca
     4441 tctgctgaca gatcgttgtc agcggaagtc cctgcgataa caggcacaga agggaagata
     4501 acgctactgc atatgcaatc gggaaacgca gcagaatcat gacaagaaag cttacgagca
     4561 acactgcgat tgccatactc tgtacactca ttatttcgtc ccctccttta caaaaatact
     4621 cttaatatga ttataaattg cttccagttc aaaaacgacc atcgctacgc cggcaagcgg
     4681 aatcgggaag tacatccaga acttggacag ccacggcatg ctgacgtaat tacccttggc
     4741 tccaatgcct gtcgcatagc tgaatccaac ggtaatcatc acgattgcca gaataaatac
     4801 tgcgacatcc gccagaatgt ccaacacctt aatcacgttc ttcggcaggt atgcgtcaaa
     4861 cgccgtcata cggatgtgtg catttctgcg gattgcaagt gccgccgaga gcactgccat
     4921 gtaggacatc agggtcagta ccacttcctc gctccatgcc ggatcgggta cgaaggaaac
     4981 atatcttccg acaacgcaga cactcgtgat tacgatatct gccgtgagca gcaatttgca
     5041 tacaaacagt acaaacttat atgccacatc atatgccggc tttattttat ctaaagtctg
     5101 aaaaatctta ggcatttttt cctccttaaa aaagtcgggg cacgggggag cagccattct
     5161 ccctgtgccc cttgaaaacg cacttacatt attgcgggtt ttccttctat caaacctata
     5221 agtgtcaatc tttctgtcga ttactgcata tcaacgatct gctgatataa ctctttgtta
     5281 ttcttggttg cctcttcgat gacatccttg caggcatcct gccacggtgt gatatcgtct
     5341 acttcaataa cattaacgcc gtctgccttt aactcctcaa ggatcttatc ctctgtctcc
     5401 tgggagatct gacggttgta ctgggaagca tactcgccgg cctcggtcag cacatcctgc
     5461 tgctccggtg tcagggaatc ccatgcctcg tctgtgataa ctacctggat tgctcctaag
     5521 gtatgtccat caagaatgat gttcggtgct acttccggga atgcatttgc ctgatagttt
     5581 gcgatcggct gttctgctgc atctaccaca ccggtctgta atgcggaata taactcaccc
     5641 atggcaacaa cggtcgggtt tgctccaagt ccttccacca tacctgtcat gatcgggtcg
     5701 ttggatacac ggatcttcat gcctgccaga tcatccattc cctcgatcgg attgacggtg
     5761 aagaaatggc ggaaaccttc ctcgccgtag aacagtccgc ggataccggt tccgttctca
     5821 tgcggctcaa gcaggaactc ctgtgccaga tcagaggttg caaagttcca gaaatgatcg
     5881 cggcttacga atgtgaacgg tacggataag agactggatt tctctccgcc gtagcttgtg
     5941 agtgcgaatg cggaaatacg tgccatatcg atggtaccgc cgccgccgag catggtgtcg
     6001 agtacatcgg actctgcacc gagaacaccg tttgcctgaa tatcaatcgt aatacttccg
     6061 ccggaaagct cttctacctt ctctttgaat gcggttgctg tctgtcctac aatggttccc
     6121 tccagcgggt ttacctctgc gtataccaga gtcactgccg gtccgaccgg ctctgttcct
     6181 gtggaatcac cggcctcggt tgccgctgcg ctatccgttg catctgccgt tgcatctgcc
     6241 gctgcatctg tgttctggct tgtcgtcgtg tctgccgcct ctggcgcttt cagtccacag
     6301 gcagtcatgg aaaccaccat tgctgcacat aacagtgcgg ataaaacttt tttcttcatg
     6361 taaaaatcct ccttcttctc ctcccgccgc gcttcatccg gcggctttgt ttatgtctta
     6421 attataaagt tctatcctca caatggaaac cgtacatttt tattaacttt agcacatttt
     6481 taacaatttt aatagttttt ttatatttat atggtcaaaa tattctattt ctttctgaaa
     6541 ttctaacgat atacagatga aatgcacaaa aagagccagt acattgactg gctctaatat
     6601 cttggaaata tcttacagtt tattttgata attcccnnnn nnnnnngttg ctctggcatc
     6661 cttcacctgt cctttttcaa cttttttatt ccatcaaacc tgcatagcca aacgaacaga
     6721 actccattta ctccgcaaat gtctctgcca ccttcacaag caggtcgcgg atcggcagat
     6781 gctgcggaca ttgtccctca cacttgccgc agcggataca gacattcgca ctgccaccgt
     6841 cagcggtatg ccgccggtaa ctgccgcggt ttgtctcatt gatcccgaac tgcacggtca
     6901 tgttgtaatc ctcaaagaca tccgggatcg gaatcttttt cggacatccc tccacgcagt
     6961 agcggcactt ggtgcaggcg atcgtcggca tggc
//
LOCUS       DBMDQJ010000023         6976 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4501_length_6976_cov_1.808873, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000023 DBMDQJ010000000
VERSION     DBMDQJ010000023.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6976)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6976)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6976
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4501_length_6976_cov_1.808873"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="ACZO9R_00885"
     CDS             complement(<1..329)
                     /locus_tag="ACZO9R_00885"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR0911660.1"
                     /translation="MELLDIVDEKGNPTGETVPREVAHREGIRHRTAHVWIFRRRGGV
                     VQILLQKRSDNKDSDPGCYDISSAGHIPAGSDYIPSAVRELKEELGVDMNGDELIYCG
                     QRRFRFE"
     gene            complement(1078..2946)
                     /locus_tag="ACZO9R_00890"
     CDS             complement(1078..2946)
                     /locus_tag="ACZO9R_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911661.1"
                     /translation="MKWKKLVTLGCVVVMTVSSVTACGSNTTENQTVEATEQSEENQS
                     DSVIVQVTAVEGDQITADVGTLTTASADASGNGAPGGEAPSGEAPGGDDSGNGAPGEA
                     PSGEAPGGDNSGNGAPGEAPSGEAPGGEAPGGDDSGNGAPGDAPSGEAPSGDAPGGQM
                     PGGSSFEASGESITFTLTDDTAITLEYLQGSDEGNADDIAVGSVLEVVLDEDNQAVSV
                     TVRNLNAGGGFGGSGEVTNGTSANTITEDTEVDSETYTSTGDDENALRVDGATVTLKD
                     ITIEKIAGSSSNTEDGDFYGLNAGLLVLNGATATITGATVNTSVTNGNGVFSYGEGTV
                     VNISDSTIRTTENNSGGIQTTGGGTMNAANLDVETQGNSAAAIRSDRGGGTVKVDGGS
                     YVTNGTGSPAIYCTADISVSDATLTANASEGVVVEGKNSVALTDCEVTGNMSNTYNGD
                     SDENIHCIMIYQSMSGDADVGEAAFSAEGGSITAKTGDMFYITNTDCEITLKDVAFTL
                     ANDVFLRVEGNSSSRGWGTEGANGGDVTLTADSQEFTGNILVDEISSLVLTMKNGTSY
                     EGAINPDGAGGTVDVTLDDNSTWTLTGDSYITSFDGDTSNITANGYHLYVNGEQVL"
     gene            complement(3071..3424)
                     /locus_tag="ACZO9R_00895"
     CDS             complement(3071..3424)
                     /locus_tag="ACZO9R_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR0911662.1"
                     /translation="MNIFLLGMAGLLYGIFVSYTGLAIPCLFRKVTGLLCPGCGVTGM
                     CVALLHLNWKGAFFCHPVLFVLLLPLTAVFICSAAGYVQNGRFRFARWQNLILYVSVA
                     ALVIFGVVRNLVRFL"
     gene            complement(3475..3816)
                     /locus_tag="ACZO9R_00900"
     CDS             complement(3475..3816)
                     /locus_tag="ACZO9R_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4234 domain-containing protein"
                     /protein_id="HIR0911663.1"
                     /translation="MIERRNIAVCIVLTLVTCGIYGIYWIVCLTNDVNTVSGDVNGTS
                     GGMVVLLTIVTCGIYGIYWAYKQGEKLDFTKNNRGIPSSNSGVLYLILQIFGFGIIAY
                     ALMQNELNKLA"
     gene            complement(3898..4338)
                     /locus_tag="ACZO9R_00905"
     CDS             complement(3898..4338)
                     /locus_tag="ACZO9R_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0911664.1"
                     /translation="MKDKYDALKLENQLCFPLYACSKEIVRRYKPYLDEIDLTYTQYI
                     AMMVLWEHGRINVKDMGALLYLDSGTLTPVLKKLEQKGYLTRERDHADERVLNVTITE
                     AGEQLKEQAVDIPRKMGGCIGLSGEDARELYRILHKVLALMEQE"
     gene            complement(4354..>4536)
                     /locus_tag="ACZO9R_00910"
     CDS             complement(4354..>4536)
                     /locus_tag="ACZO9R_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR0911665.1"
                     /translation="HPLATIVESMLERTHLDYKETPDIKWNFTKFLIDRDGNVVERFE
                     PTADMDVVREKIENYL"
     assembly_gap    4537..4546
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4547..4837)
                     /locus_tag="ACZO9R_00915"
     CDS             complement(<4547..4837)
                     /locus_tag="ACZO9R_00915"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012479.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR0911666.1"
                     /translation="MVVNTATKCGFTPQYDELQSIYEQYGPQGFEILDFPCDQFEHQA
                     PGTNEEIVAFCDVNFGITFTHYGKIDVNGEHALPLYQYLKSQKGFAGFDEEHP"
     gene            complement(4867..5184)
                     /gene="trxA"
                     /locus_tag="ACZO9R_00920"
     CDS             complement(4867..5184)
                     /gene="trxA"
                     /locus_tag="ACZO9R_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR0911667.1"
                     /translation="MVKKIQNNNMDEALQAKAAVVDFSAVWCGPCQMLAPVLEELSGD
                     LDGKVAFYNADTDENTQLAIANGITNIPALLFLKDGKVVGKNVGFMPKPELEAWVKGC
                     MEQ"
     gene            complement(5297..6178)
                     /locus_tag="ACZO9R_00925"
     CDS             complement(5297..6178)
                     /locus_tag="ACZO9R_00925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0911668.1"
                     /translation="MERVDIAIIGTGPAGVSAAITAKVRNKSVLLIGSRELSEKMSKA
                     HQILNYPGLPEVTGADLAAGYKKQLAGLGIEVTEKRVNVVYAMGDYFGIQASGEILEA
                     STVILAAGIVMGKPFPGENELLGSGVSYCATCDAQFFRGKEVAVIGYGREAEQEADYL
                     AEIVGKVHYFPMGKEEPQLSDAIEVVHERPKAIAGTQTVSALETDAGTHPVDGVFILR
                     DAIAPSQLVPGLATEENHVKVNLQMETNLPGLFACGDIAGKPYQYIKAAGQGNVAALS
                     AVEYLDKKKREKQTDIG"
     gene            complement(6210..6752)
                     /locus_tag="ACZO9R_00930"
     CDS             complement(6210..6752)
                     /locus_tag="ACZO9R_00930"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007594116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HIR0911669.1"
                     /translation="MGIYDYSVERPDGTTQPLSAYEGKVLLIVNTATGCGFTPQYREL
                     EEFYRKYHDQGLEILDIPCNQFAGQTPGTDEEIHEFCTLKYHTGFPQMKKSDVNGEHQ
                     IPLYAYLKSQKGFAGFGKGAKAAAMGIMLRKIDKNYKENSDIKWNFTKFLVDRQGTVV
                     VRVEPTGDMNELERQILALL"
BASE COUNT         1494 a         2069 c         1418 g         1985 t
ORIGIN      
        1 ctttcaaacc ggaaccggcg ttgtccacaa taaatcagtt catcgccgtt catgtccaca
       61 cccagttctt ccttcagctc ccgcaccgcg gaaggaatat aatcactccc tgcaggaata
      121 tgaccggcac ttgaaatgtc ataacagccc ggatctgaat ccttattgtc gctccgtttc
      181 tgcaaaagga tctgcactac gccgccgcgt ctgcgaaaaa tccacacgtg cgccgtacgg
      241 tgccggattc cctcacggtg tgccacctcc cgcggcactg tctctcctgt cggatttccc
      301 ttttcgtcca caatatccag cagttccatc tctgctcccc tccagtatga ttatattctt
      361 ttcattcact atagcacact tttgctgcct ttttccacat atccgaaatt cttccggagc
      421 gtctgatata attccactaa aatacatagt cgctctcttg aaaaaaagct gccgcattca
      481 gcagatcgtc ctgtttgacg agataagtaa tattataaga cgtacactgt tacgatttcg
      541 ggtggaatgc ctagggaaca ttctgttcaa aaaatgcggc atttctaacc agccggtggc
      601 tgcatctgcg atgatccgtg tctttccaag tttggagcac tccgacgggg catgtccgca
      661 ctccggaaga caatttgtag gacttttgtg tgtgtccctt atggcacctc tgaacaaaac
      721 ataaccgcgc gaagccgctg tctgagtcgg catctgccct attttcaata gcagatgccg
      781 gcgagttcgg ttcgcgcggt tttatattgt ctgtgaagag gtgccattag ggacaccaga
      841 aagtccgccc attgtctccg gtgtgtcgga catgcacccg tcagtgtcca aacgtggaag
      901 agacacggaa tcagatgcag tcatccggct ggaagaaata cacacatttt tctccaacga
      961 atgtttccta tgcagttcca cccggaatct gcatcatgtg cttcctgtaa tattacttat
     1021 catcaactgc cacctgtgat atgtggcagc ctctttttca actgcctctc caatctgtta
     1081 cagtacctgc tctccgttca cgtacagatg atatccgttt gccgtaatat tggaggtatc
     1141 accgtcaaag cttgtgatat agctgtcacc ggtcagtgtc caggtggagt tgtcatccag
     1201 ggtcacgtca accgtaccgc cggctccgtc cggattgatg gcgccctcgt agctggtgcc
     1261 gtttttcatg gtaagcacca ggcttgagat ctcatccacg agaatattgc ccgtaaactc
     1321 ctgtgaatcc gccgtcagtg tcacatctcc gccgtttgcg ccctctgtgc cccatccgcg
     1381 ggaggaagag ttgccctcca cgcgaaggaa tacatcattg gcaagtgtaa atgcgacgtc
     1441 cttgagtgta atctcacaat ccgtattggt aatatagaac atatctcccg tcttcgccgt
     1501 aatgctgccg ccctctgcag agaatgctgc ctctccgaca tctgcatcac cggacatgct
     1561 ctggtaaatc ataatgcagt gaatattttc atcagaatcg ccgttataag tgttgctcat
     1621 atttcctgtc acttcacagt cggtcaatgc aacggaattc ttgccctcta ccacaacgcc
     1681 ctcgctggca ttcgctgtca gagtcgcgtc tgacacgctg atatctgctg tacagtagat
     1741 tgccgggctt cccgttccgt ttgtcacata ggaaccgccg tccaccttga ccgtaccgcc
     1801 gccccggtcg cttcggattg cggctgcgga atttccctgt gtctcgacat ccagatttgc
     1861 agcgttcatg gtaccaccgc cggtcgtctg gataccgccg gaattatttt ctgtcgtgcg
     1921 gattgtggaa tccgaaatat ttacaaccgt gccctcgcca tagctgaaca caccgttacc
     1981 gtttgtcacg ctggtattga ccgtcgctcc cgtaatcgtt gccgttgcgc cgtttagcac
     2041 cagaagtccg gcattcagtc cgtaaaaatc tccatcctct gtgttcgatg aggagccggc
     2101 tatcttctcg atcgtgatat cttttaaggt aacagtcgct ccgtcgacac gaagtgcgtt
     2161 ctcatcatca ccggtggatg tgtaagtctc actatccacc tcggtatcct cggtaatcgt
     2221 atttgcagaa gttccgtttg tcacctcacc gcttccgcca aatccgccgc ctgcatttaa
     2281 gttgcgcaca gtgacagaca ctgcctggtt gtcttcgtcc agaactacct ccaatacgct
     2341 gccgactgca atgtcatctg catttccctc gtcgcttccc tgcagatact ctaatgtgat
     2401 cgccgtatcg tctgtcaggg taaaggtaat gctctcaccg gaagcctcaa aactgctgcc
     2461 gcccggcatc tgaccgcccg gtgcgtctcc acttggtgct tctccgcttg gtgcgtctcc
     2521 cggcgctccg tttccactgt cgtctccgcc cggtgcttct ccgcccggtg cttctccact
     2581 tggcgcttct cccggtgctc cgtttccact gttgtctccg cccggtgctt ctccacttgg
     2641 cgcttctccc ggcgctccgt ttccactgtc gtctccgccc ggtgcttctc cgctcggtgc
     2701 ttctccgccc ggtgctccat ttccggatgc atctgcgctt gcggttgtaa gtgttcctac
     2761 atccgctgta atctgatcgc cttcaactgc tgtaacctgc acgatcacag aatcgctctg
     2821 gttctcctca ctctgctctg ttgcttctac cgtctggttc tctgttgtat tactgccgca
     2881 tgctgttacg gatgataccg tcataactac gacgcagccc aatgttacta attttttcca
     2941 tttcatgatc ttcattcctc tctgactctg tcaatgtctg ttttcttttg atagggttag
     3001 tatagaaagc caatgtgaag atcatgtgtt gaccacgtta aattattctg aatctatttt
     3061 ttcctgtttt tcacaaaaaa cgcaccagat tgcgcaccac accgaagatt acaagtgcgg
     3121 cgacgctgac gtaaagaatg agattctgcc agcgggcgaa ccggaatctg ccattctgca
     3181 cataccccgc cgcactgcag ataaatactg ccgtaagtgg aagcagcaac acaaacagca
     3241 ccggatgaca gaaaaaagcc cccttccagt taagatgcag gagagccaca cacattcccg
     3301 tcacaccgca gcccggacac aacagtcccg tcactttccg gaacagacag ggaatggcga
     3361 gccctgtgta cgacacgaat attccgtaca acagtcccgc cattcccagt aaaaatatat
     3421 tcatcagcaa ttttttcaaa cgtgcttttt tcgtcatcct acatttccga tctgttatgc
     3481 cagcttattc agctcgttct gcatcaatgc atatgcaata attccaaatc caaaaatctg
     3541 gagaatcaga tacaaaacac cgctgttgct tgacgggatt ccacggttat tctttgtaaa
     3601 gtcaagcttc tctccctgct tatatgccca gtaaatgccg taaattccac aggtcacaat
     3661 cgtcagcagc accaccatac cgccggatgt tccattcaca tcaccagaaa ccgtatttac
     3721 gtcattggtc agacacacga tccagtagat gccataaatt ccgcaggtta caagtgtcag
     3781 tacaatacac accgcaatat ttcttctctc aatcataaca aactctcctt taaataagta
     3841 tgaattcatt atatcagttt tttgtatatt gtctactgtt ttcataccgt ggccatttca
     3901 ttcctgctcc atcaatgcaa gcaccttgtg caggatgcgg tacagctccc tcgcatcctc
     3961 cccggacagt ccgatacagc cgcccatctt ccgcggaatg tcgaccgcct gctcctttag
     4021 ctgctctccc gcctccgtga tcgtcacatt taacacacgc tcatctgcgt gatcacgctc
     4081 tctggtgagg tagcccttct gctccagctt ctttaacacc ggtgtcagcg tccccgaatc
     4141 caggtacaaa agcgccccca tatccttcac attgatcctg ccatgctccc acagcaccat
     4201 catcgcaata tactgcgtgt aggtcagatc aatctcatcc agatatggct tgtagcgccg
     4261 cacgatctcc ttggagcatg cgtacagcgg aaaacagagt tgattttcta attttaaagc
     4321 atcatactta tctttcattc cggcacctgt cgcttataaa tagttttcaa tcttttcgcg
     4381 gacaacatcc atatccgccg tcggctcaaa acgctccacc acgtttccgt ctctgtcaat
     4441 caaaaacttg gtaaagttcc atttgatgtc cggtgtctcc ttataatcca gatgcgtgcg
     4501 ctctaacatg ctttccacga tcgtcgctaa cggatgnnnn nnnnnncgga tgttcctcgt
     4561 caaatcccgc aaatcccttc tgcgacttca gatactgata cagcggcagc gcatgctccc
     4621 cgttgacatc gatcttcccg tagtgcgtga atgtgattcc aaagttgaca tcgcagaatg
     4681 caacgatctc ctcgttggtt cccggtgcct gatgttcaaa ctggtcgcac ggaaaatcca
     4741 gaatctcaaa tccctgcgga ccgtactgct cgtaaatact ctgcagttca tcgtactgcg
     4801 gcgtaaatcc gcatttggtc gccgtgttga ctaccaggac aacctttccc tatcttctgt
     4861 atgctgttac tgctccatac atccttttac ccatgcttcc agttccggct tcggcatgaa
     4921 tccgacattc ttgccgacca ccttgccgtc ctttaagaat aaaagcgccg gaatgtttgt
     4981 gatgccgttt gcgatcgcaa gctgcgtatt ctcatcggta tctgcattgt aaaatgccac
     5041 cttaccgtcc agatcgcctg acagttcctc cagtaccggg gcaagcatct ggcacggtcc
     5101 gcaccatact gccgaaaagt ccaccactgc tgcctttgcc tgtaacgctt cgtccatatt
     5161 gttgttctga atcttcttaa ccatgataat cctccttaga tctgtgtatt atagtttgat
     5221 cgctgttcct gcttttgttt ctgtctctgt cttttcttgt tttttctgtg cttttacttt
     5281 ttctgtggtt ccgcctttac ccgatatctg tctgtttctc tctttttttc ttatccagat
     5341 actctaccgc ggacagcgcc gccacatttc cctgtcctgc cgccttgata tactgatacg
     5401 gctttccggc aatgtctccg catgcaaaaa gtcccggaag gtttgtctcc atctggagat
     5461 tgacctttac gtggttctcc tccgtcgcaa gtcccggcac gagctgtgac ggtgcgatcg
     5521 cgtctcttaa gataaatacg ccatccaccg gatgcgtacc cgcgtcggtc tcaagcgcac
     5581 ttaccgtctg cgtgcctgcg atcgctttcg gccgctcatg cacaacctct atggcatccg
     5641 acagctgcgg ctcctctttt cccatcggga aataatgcac ttttccaaca atctccgcca
     5701 gatagtccgc ttcctgctcc gcctctctgc cgtatccgat cacagcgact tcctttccgc
     5761 ggaagaactg cgcatcgcag gtcgcgcagt agctgacacc gcttcccagc agctcgtttt
     5821 ctcccgggaa cggttttccc atgacgattc ctgccgccag aatgacggtg gacgcctcaa
     5881 gaatctcgcc ggacgcctgg attccaaaat aatctcccat cgcatacacc acattcacgc
     5941 gcttctccgt cacctcgatg ccaagacctg ccagctgttt tttgtacccg gcagccaggt
     6001 ccgctccggt gacctccggc agtcccggat agtttaaaat ctgatgcgct ttgctcatct
     6061 tctcgctgag ttccctgctg ccgattaaca gcacgctttt atttctcact ttcgcggtaa
     6121 tcgccgccga cacacctgcc ggccctgttc caataatggc aatatccact cgttccatac
     6181 cattctccat tccctgtatc gctctgcatc tataataatg caagaatctg tcgttccagt
     6241 tcgttcatat ctcctgttgg ttcaactctt accactaccg tgccctgcct gtccacaagg
     6301 aacttcgtaa aattccattt gatgtcggaa ttctccttat aattcttatc tattttgcgg
     6361 agcattattc ccatcgccgc cgctttcgcc cctttcccga atccggcaaa tcccttctgg
     6421 gattttaaat atgcatacag cggaatctga tgttcgccgt tgacgtcgga tttcttcatc
     6481 tgagggaacc cggtgtgata cttcagcgta cagaactcat gaatctcctc atccgtcccc
     6541 ggcgtctgtc ccgcgaactg gttgcacggg atatccaaaa tctcaagtcc ctgatcatga
     6601 tacttccggt aaaactcctc cagttcccgg tactgcgggg tgaatccgca tccggtcgcc
     6661 gtattcacaa tcagaagtac ctttccctca taagcggaca acggctgcgt tgtcccgtcc
     6721 ggccgctcca cagaataatc ataaatgccc atctcttacc ctcctttctc tttgtcatta
     6781 aaaatgaatc tttttaaatt gtttcatttt taattgtgta caatcttttt gtgtttagat
     6841 tgtacacaat ctattcgagg atgtcaagca ttattttatc aatcatcgtt ctctgtgccg
     6901 gattgtcgta accggcaaaa tgaaacattg cattttttca tcgaaagctc tatgatagtc
     6961 agcagacaat gggatt
//
LOCUS       DBMDQJ010000024         6950 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4517_length_6950_cov_1.330851, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000024 DBMDQJ010000000
VERSION     DBMDQJ010000024.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6950)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4517_length_6950_cov_1.330851"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="ACZO9R_00935"
     CDS             complement(<1..639)
                     /locus_tag="ACZO9R_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911670.1"
                     /translation="MEAEQVKTKKVSAADGIPEKTGRKKWSVLFLESLLKEAKEREAE
                     GEKARPAVDGESWIRPGRDTFEHSEDPEELWCESYEDIMGVVRMVGKWKANILYQIAN
                     AILRDYHEKKLDEDGVGELFGLCCQKCKDTGGGTGRKTEPQITETVLEFFLRANARMS
                     VFSMERAGKRLTARCGLSWSGTTYYDAWYFYRCAQMHRRLFVVAGRIAAELGE"
     gene            complement(642..1403)
                     /locus_tag="ACZO9R_00940"
     CDS             complement(642..1403)
                     /locus_tag="ACZO9R_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911671.1"
                     /translation="MRIYNRDQSAILGARNNRLAKMRAFSSSTKTRASSKGTTQSAPK
                     SKNYRTSAWMQFLNQFQNGTAVSGDKIVENQKKSYDYEITGLAAERVETHMKRFLEEG
                     EKSLYQEGEDAGKQLTKEMESFVSDYNMMIRKIGDLSDSVDETYAKKLKMEISARETA
                     LKQIGITQEKDGTLSFDKTVFQTVKPEAVQKIFGDKSTLSNRVQVLASGVKSNCRKQA
                     AALFATSYTTSGNYSRYASDSNQTRQTGSRYNAKG"
     gene            complement(1434..2012)
                     /locus_tag="ACZO9R_00945"
     CDS             complement(1434..2012)
                     /locus_tag="ACZO9R_00945"
                     /inference="COORDINATES: protein motif:HMM:NF016296.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR0911672.1"
                     /translation="MLYIAACGNEASVRKYLKEMEGEYVREDVQILCGDEIEGSGTMP
                     DVLFLSDSGIEECQKKIPVWFRRKAFDNAGELKQRKAGAERKRPLLIKTDGTYCCVDQ
                     KTILYAESVGRKVVLHTNTGIIAYYARMKEVEEILGNGFFRCHRGYLVNFAAVKSFEV
                     GTILLKNGESILMAKQKFNDFASAYETYLNER"
     gene            2196..2567
                     /locus_tag="ACZO9R_00950"
     CDS             2196..2567
                     /locus_tag="ACZO9R_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0911673.1"
                     /translation="MAWTLDSDRPIFLQIVERIQTDIVSGRYQPGDKLPSVRDLAAEA
                     SVNPNTMQKAFAELERTGLVYSRRTSGRFITEDCHMIEQLKASLATEKMEEFLAQMRQ
                     LGFQNDEILSLMNQTMKGEKQ"
     gene            2564..3262
                     /locus_tag="ACZO9R_00955"
     CDS             2564..3262
                     /locus_tag="ACZO9R_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911674.1"
                     /translation="MSTLLECKDLTKCYGKKTAIDHINLAIESGHIIGLLGPNGSGKT
                     TLIKMINGLLAPTSGLIFMQQNPIGAESKKHISYLPDHTYLNMNERVRDIIRYFSDFY
                     EDFDEKRAYDMLGKLQIDASDRLKTMSKGTKEKVQLILVMSRHADLYILDEPIAGVDP
                     AARDFILNIILSNYEPEASILISTHLIADIENILDEVIFIDHGQIRLTASVDDIRMNN
                     GKSVDTLFREVYRC"
     gene            3256..4098
                     /locus_tag="ACZO9R_00960"
     CDS             3256..4098
                     /locus_tag="ACZO9R_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911675.1"
                     /translation="MLKKLFKYEFKSTAKIMLILYAILIVTTAVGSVVLYSLDLDQAG
                     ESKLASILSVSAIVLYILAIFAVLIVMYVYLCVHFYRTMYSAQGYLTHTLPVKALAPF
                     HVKLITSFVWMFLSMALMTVSIVVLIASASHGTAWQDFTSAWDEVFGQDVFSFRFIFQ
                     MVLSVICSCLTYLLWVFASASIGQLFSSNKVAASLVAGIILYFMQQIFSMILMFGTGM
                     FQSGDNSVNFFAVTTTIDNTTTSTTSILPSSLFATSNIYSIIVIVILYLVCARIITKH
                     LNLE"
     gene            complement(4734..6119)
                     /gene="gltA"
                     /locus_tag="ACZO9R_00965"
     CDS             complement(4734..6119)
                     /gene="gltA"
                     /locus_tag="ACZO9R_00965"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HIR0911676.1"
                     /translation="MDVMTRVPVREQDPQVRAANFEEVCLGYNAEEAMAEAARCLNCK
                     NAQCMKGCPVSINIPAFIAQVKEGNFEEAYHIISESSALPAVCGRVCPQENQCEGKCI
                     RGIKGEAVAIGKLERFVADWAREHGIKPKKAEKLNGHKVAVIGSGPAGLTCAGDLAKL
                     GYDVTIFEALHEAGGVLVYGIPEFRLPKQKVVAAEVENVKALGVKIETNVVIGKSVTI
                     DELMENEGFEAVFIGSGAGLPRFMGIPGEQAMGVFSANEFLTRNNLMKAFLEEYDTPI
                     KAGKKVIVVGGGNVAMDAARTAKRLGAEVHVVYRRGEEELPARKEEVHHAKEEGIIFD
                     LLQNPTELLVDENGCVKGARVIKMELGEPDASGRRSPVEILGSEYEIEADTVIMSLGT
                     SPNPLISSTTKGLETNRRKCIVAEEENGATSKSGVFAGGDAVTGAATVILAMGAGKAA
                     AKGIDAYLNTK"
     gene            complement(6204..>6950)
                     /locus_tag="ACZO9R_00970"
     CDS             complement(6204..>6950)
                     /locus_tag="ACZO9R_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HIR0911677.1"
                     /translation="IPLTICDYNREAGTITIVFQTVGASTQRMVSLEQGDAFADFVGP
                     LGCPSDLIDEDIEELKKKKIVFIAGGVGTAPVYPQVKWLHEHGVDCDVIMGSRNKDLL
                     ILEDEMRAVAANLYVTTDDGSYGFHGTGTAQLQELWDQGVRYDHCVAIGPMIMMKFVC
                     KLTKELGIPTVVSMNPIMVDGTGMCGACRLVVGDEVKFACVDGPEFDGHLVDFDQAMK
                     RQQQYKTEEGRAMLKLTEGDTHHGGCGQCG"
BASE COUNT         1571 a         2053 c         1432 g         1894 t
ORIGIN      
        1 ctctcccagt tccgctgcaa ttcttccggc aaccacaaaa agccgtctgt gcatctgtgc
       61 acagcggtaa aaataccagg cgtcatagta cgtcgtgccg gaccagctta agccgcagcg
      121 cgccgtcagg cgctttcccg ctctttccat ggaaaataca ctcatccgcg cattggcacg
      181 caggaaaaat tcaaggacag tctccgtgat ttgcggctcc gtcttcctgc cggtgccgcc
      241 tcccgtgtcc ttacacttct ggcagcacag gccaaacagc tctcccacac cgtcctcgtc
      301 cagctttttt tcgtgatagt cccgcaggat ggcattggca atctgataga gaatgttggc
      361 tttccacttc cccaccatgc gcacgacccc catgatatcc tcataggatt cacaccagag
      421 ttcctccgga tcctccgaat gttcaaaggt gtctcttcca gggcggatcc agctctcgcc
      481 gtcgactgcc ggtctggctt tttccccttc cgcctcccgc tccttcgcct ctttgagcaa
      541 tgactccaga aagagaacgc tccacttttt tctgccggtc ttctccggaa tcccgtctgc
      601 tgcgctgacc ttttttgtct ttacctgctc tgcttccatt cctaaccttt cgcattgtag
      661 cggctgccgg tctgccgcgt ctgattactg tccgacgcat agcggctgta attgccgctg
      721 gtcgtataag aagttgcaaa aagcgctgcc gcctgttttc tgcagttgct cttcacaccc
      781 gaagccagca cctgcacgcg gttcgacagc gtgctcttat ccccgaaaat cttctgcacc
      841 gcctccggtt ttactgtctg aaacaccgtc ttatcaaacg acagcgtgcc gtctttctcc
      901 tgcgtgattc cgatctgttt cagggcggtc tcccgcgcgg agatctccat tttcagcttc
      961 tttgcatacg tctcgtccac actgtccgac aagtcaccaa tcttgcggat catcatattg
     1021 taatcggata caaaactttc catttccttc gtaagctgtt ttccggcgtc ctctccctcc
     1081 tggtaaaggg atttttctcc ctcctccagg aaacgcttca tatgcgtctc cacacgctcc
     1141 gccgccagtc ccgtgatctc ataatcgtag ctttttttct gattctccac gattttgtcg
     1201 ccggatacgg ctgtcccgtt ttgaaactga tttaaaaact gcatccacgc agatgtcctg
     1261 tagtttttac tctttggcgc gctctgcgtc gttcctttgg atgatgcgcg tgtctttgtg
     1321 ctgctggaaa acgcacgcat ctttgccagc cggttgttcc tcgcacccag aatggcagac
     1381 tggtctctgt tataaattct catactcatt ttccccgttt cttttctgta tccctatcgt
     1441 tcgttaagat acgtctcata agccgacgca aaatcattaa acttctgttt cgccatcaga
     1501 atggactccc cgttcttcag caggatcgtt cccacctcaa aactttttac cgcggcaaaa
     1561 ttcaccagat acccgcgatg gcagcggaaa aacccgttgc ctaaaatttc ttccacctcc
     1621 ttcattctgg cataatatgc aatgattccc gtgtttgtgt gaagcaccac cttacgcccc
     1681 acactctctg catagagaat ggttttctga tccacacagc aataggttcc gtctgtttta
     1741 atcagcagcg ggcgcttcct ttctgcccct gcttttctct gtttcagttc tcccgcattg
     1801 tcaaacgctt ttctgcggaa ccataccgga atctttttct ggcactcctc tatgccacta
     1861 tccgaaagga aaagaacatc cggcattgtg ccgcttccct caatttcatc tccgcaaaga
     1921 atctgtacat cctctcttac atattcgcct tccatctctt ttaaatactt acgcacggat
     1981 gcctcattcc cgcacgccgc aatatataac actcatattc ctccccgctt tcgcatccat
     2041 gctctgtttt ctactttata tatcggtcat atttttcatt ctttcatttt tcgtaacgaa
     2101 atgcgctgtt tttgtcatca aaaaaacagt tgacacgctt actgtattat tgtattatct
     2161 tattagtaca gatgatttag aaaggagatg acactatggc atggaccctt gattctgatc
     2221 gtcccatctt tttacagatc gtggaacgaa tccagacgga catcgtttcc ggccgctacc
     2281 agccgggaga taagcttccg tccgtacgcg atctcgcagc ggaagcatcc gtcaacccga
     2341 acaccatgca aaaggccttt gcagaacttg aacgcaccgg tctggtctat tccagacgga
     2401 ccagcggacg ctttatcacg gaggattgtc atatgataga acagttaaaa gcctctcttg
     2461 ctaccgagaa aatggaagaa tttttagcac agatgcggca gctcggcttt caaaacgatg
     2521 aaatcctgtc gctcatgaat cagacgatga aaggagaaaa acaatgagca ctttgttgga
     2581 gtgtaaagat ctgaccaaat gttacggcaa aaaaaccgcc atcgaccata ttaacctcgc
     2641 cattgagagc ggacacatca tcggcctgct cggaccgaac ggaagcggca aaaccaccct
     2701 gatcaagatg atcaacggtc tgcttgcccc cacctccggt ttgatcttca tgcagcagaa
     2761 tccgatcggc gcggagagca aaaaacatat ctcctatctg ccggatcaca cttatctgaa
     2821 tatgaacgag cgggttcgcg atattatccg gtatttttcc gatttctatg aagattttga
     2881 tgagaagcgc gcctacgaca tgttgggaaa gcttcagatt gacgcctcgg accgtttgaa
     2941 aaccatgtcc aagggaacca aagaaaaagt gcagctgatt ctcgtgatga gccgtcatgc
     3001 agatctgtat attttagatg agccgatcgc cggcgtcgat ccggccgcac gcgattttat
     3061 cttaaatatc atcctgtcga attacgaacc ggaggcttcc attctgatct cgacgcatct
     3121 gatcgccgat atcgagaata ttctggacga agtcatcttc atcgatcacg gtcagatccg
     3181 gcttaccgca tccgtggatg atatccgcat gaacaacgga aagtccgtcg atacactttt
     3241 cagggaggta tacagatgtt aaaaaaatta tttaaatacg aatttaagag cacggcaaag
     3301 atcatgttga tcctgtatgc cattctgatc gtcaccaccg ccgtaggttc cgtggtgctg
     3361 tattccttag acctggatca ggcgggtgaa agcaaactgg catccattct gtccgtctcc
     3421 gcgattgtcc tttatatcct tgccattttt gctgtcctta tcgtgatgta cgtgtacctg
     3481 tgtgttcact tctaccgcac aatgtattct gcccagggat atctgacaca caccctcccg
     3541 gtaaaagcac ttgctccctt tcacgtaaaa ctgataacct ccttcgtgtg gatgtttctc
     3601 tccatggctc tgatgactgt ctccatcgta gtgcttatcg ccagcgcctc ccacggaacc
     3661 gcatggcagg attttacttc cgcctgggat gaggtattcg gtcaggatgt attttccttc
     3721 cgtttcatct tccagatggt attgagcgtg atctgctcct gcttaaccta cctgctctgg
     3781 gtgtttgcaa gcgcttcgat cggacagctt ttctccagca ataaagtggc tgcctccctc
     3841 gtcgcgggca tcatcctcta ttttatgcag cagattttct ccatgatcct catgtttgga
     3901 accggtatgt tccagtcggg agataattcc gtgaatttct ttgccgttac cacaacgatc
     3961 gacaatacga ccacatcgac gacaagcatc ctgccgagtt cactgtttgc caccagcaac
     4021 atttactcga tcatcgtcat tgtcattttg tacctggtgt gcgccagaat catcacaaaa
     4081 catctgaatc tcgaataacg ttctatcagg ggctgccact taagaccggc aacgcctctg
     4141 gatgtgataa gtaatattat cagaagatca caccgtggga tttcagatgg aaatgtagat
     4201 gggatattcg ttggagaaaa atgtgagtat ttcttccagc tgaatgtttg catcgaattc
     4261 cgtgtctctt ccacgtttgg acactcacgg agtgtgtccg acaagtcgca gacaatgagc
     4321 ggactttctg gtgtccctaa tggcacctct tcatagacaa tataaaaccg cgcgaaccga
     4381 aactcgccgg catctgctat tgaaaatatg gcagatgccg gctcaaacat cggcttcgcg
     4441 cggttatgtt ttattcagag gtgccaataa gggacacgca caaaagtcct aaaattgtct
     4501 gctgacttgc ggacacagtc cgtagagtgc tccaaactta gaaagacacg gatcatcgcg
     4561 gatgcaaaca ttctgttggt tagaaattcc acattttttg aaccgaattt ccatctgcat
     4621 ttccacatga aatccccaag tgtgcctctt ataatattac ttatctaata tttcttatgc
     4681 atgaaaggag gctgccactc atgtgacagc cccttcccga atctattacg tatttatttt
     4741 gtgttcaagt atgcgtcaat tcccttcgca gccgcttttc ctgcacccat tgccaggatg
     4801 accgttgcag ctcctgtcac ggcatctccg ccggcaaata caccgctctt ggaggtagcg
     4861 ccgttttctt cctctgccac gatacacttt ctgcggttcg tctcaagtcc ctttgtcgtg
     4921 gaggagatca gcggattcgg agaagttcca agcgacatga tgaccgtgtc cgcctcaatc
     4981 tcatactcag accccagaat ctccaccgga cttcttctgc cggaggcatc cggctcgcca
     5041 agttccatct tgatgactct tgcgcctttt acacagccgt tctcatccac taaaagctcc
     5101 gtcggattct gtaacaggtc aaaaataatg ccctcctcct ttgcatgatg cacctcttcc
     5161 ttacgcgccg gaagttcttc ctcaccgcgc cggtacacga catgtacttc cgcaccaaga
     5221 cgcttagccg ttctcgcagc gtccattgcc acgttaccgc cgccgacaac gatcactttc
     5281 tttcccgcct tgatcggagt atcgtactcc tctaaaaatg ccttcatgag gttgtttctc
     5341 gtcaaaaatt catttgccga aaatacgccc attgcctgct cgcccggaat acccataaaa
     5401 cgcggaagac ccgcgccgga accgataaat accgcctcaa atccctcatt ctccatcagc
     5461 tcatcgatcg tgacagattt gccgatgaca acgttcgtct cgatcttcac gccgagcgcc
     5521 ttgacattct ccacttctgc cgcaacgacc ttctgcttcg gcagacggaa ttctggaata
     5581 ccatagacaa gcacaccgcc cgcctcatgc agcgcctcaa aaatggtcac gtcatagccc
     5641 aattttgcca gatcaccggc gcaggtaagt cctgcaggac cggaaccgat gacagccacc
     5701 ttatgaccgt tcagcttctc tgccttcttc ggcttgatgc catgctctct cgcccagtct
     5761 gctacaaaac gctccagctt accgatcgca actgcctcac ccttgatgcc gcggatacac
     5821 tttccttcac actggttctc ctgcggacat acacgtccgc agactgccgg aagtgcggat
     5881 gactcactga taatgtggta agcctcctca aaattgccct cttttacctg cgcgataaac
     5941 gccggaatat tgatggatac cggacagccc ttcatgcact gtgcattttt gcagtttaag
     6001 cagcgcgctg cctctgccat tgcctcctcg gcattatatc ccaaacatac ctcctcaaag
     6061 ttcgctgctc gcacctgcgg gtcctgctca cgaaccggta ctcttgtcat tacgtccata
     6121 ggaacctcca tactctgtat tttctatccg gcagatgccc cagttctctc tgcctccacc
     6181 gtctaacgat cacttctgcc ttctcagccg cactgtccgc agccgccgtg atgggtatct
     6241 ccctccgtca gctttaacat tgctctgccc tcttctgtct tatattgctg ctggcgcttc
     6301 atcgcctggt cgaaatccac cagatgtccg tcgaattccg gtccgtccac gcaggcgaac
     6361 ttcacttcgt cgccgacaac cagacggcat gcgccgcaca ttccggtacc gtccaccatg
     6421 atcgggttca tggaaaccac cgtcggaatt cccagttctt ttgtcagctt acagacaaac
     6481 ttcatcataa tcatcgggcc gattgccaca cagtgatcat agcgcacgcc ctgatcccag
     6541 agttcctgca gctgcgccgt gccggttccg tgaaagccgt aggagccgtc atccgtcgtc
     6601 acatagagat tcgctgccac tgcccgcatc tcatcctcaa gaatcagaag atccttattg
     6661 cgggatccca taatcacatc acaatccaca ccatgctcat gcagccactt tacctgcgga
     6721 tacaccggtg cggtaccgac gccgcccgcg atgaacacaa tctttttctt cttcagttcc
     6781 tcgatatcct catcaatcag atcagaagga catccaagcg gtcccacgaa atctgcgaac
     6841 gcatccccct gctccaagga taccatacgc tgtgtggaag ctcccaccgt ctgaaatacg
     6901 atcgtgatcg taccggcttc cctgttataa tcacagattg tgagcggaat
//
LOCUS       DBMDQJ010000025         6935 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4529_length_6935_cov_1.805824, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000025 DBMDQJ010000000
VERSION     DBMDQJ010000025.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_6935_cov_1.805824"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            186..2651
                     /locus_tag="ACZO9R_00975"
     CDS             186..2651
                     /locus_tag="ACZO9R_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR0911678.1"
                     /translation="MKFGYFDDAKKEYVITSPRTPLPWINYLGSKDFFSLISNTCGGY
                     SFYKDAKLLRLTRYRYNNVPFDSNGHYYYIKEGDTIWNPGWMPTKTELDSYECHHGMG
                     YSTFRSSKNDLSAELTAFVPVDDSCEINKLTLTNNGSAPKTFSVFSYVEFCLWNAMDD
                     MTNFQRNFSTGEVEVHGSAIYHKTEYRERRNHYALYAVNAPIAGFDTDRDSFLGAYGE
                     NSAPEVVVTGASKDSMASGWAPVGSHHLSVSLAPGESKTFVFVLAYIENPIDQKWVGR
                     PEDGVINRAPAEALMARYDTTEKADAALAALKEYWDKLLSHFTISSSEKKLDRMVNVW
                     HQYQCMVTFNMSRSASYFESGIGRGMGFRDSCQDLLGFVHLIPDRARERILDIAATQF
                     EDGSAYHQYQPLTKKGNSDIGSGFNDDPLWLIAGAAAYIKETGDYTILDEMTPYDSDP
                     SKATDFMEHLRRSFHYTMEHLGPHGLPLIGRADWNDCLNLNCFSTEPGESFQTFGPSE
                     GPNAESVFIAGMFVRYGKDYAAICRHRGMDDEAAVAEKAIADMEKTVLDAGWDGEWFL
                     RAYDHYKNKVGSKECEEGKIFIEPQGFCVMAEIGLKEGNCLKAMQSVEKYLDTKYGIV
                     LLQPAYHRYHVELGEISSYPPGYKENAGIFCHNNPWISIAETVVGRGNRAWQVYTRTC
                     PAYIEDISEVHRTEPYVYSQMIAGKDAKNFGEAKNSWLTGTAAWTFLDVSQYILGIRP
                     DYDGLVIDPCIPSSLDGFTARRDFRGTTYHVTVKNPNHVEKGVISMTVDGKDVDVSGI
                     SGGMIPIALADGKKDVNVEVVMG"
     gene            complement(3338..5116)
                     /gene="pyk"
                     /locus_tag="ACZO9R_00980"
     CDS             complement(3338..5116)
                     /gene="pyk"
                     /locus_tag="ACZO9R_00980"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HIR0911679.1"
                     /translation="MLRKTKIVCTLGPSTDDENVMRSLIEEGMNVARFNFSHGGHEEQ
                     FGRLTMLRKLREEMKRPVAALLDTKGPEIRLKEFASGKVELKNGQTFTLTTEDIVGDE
                     SRVAITYKDLPDDVKPGDTILIDDGLIGLEVKKIQKVSGTKADAEGKMPMDIVCTVMN
                     GGMVSNKKGVNVPNVELSMPYISEKDYGDIVFAVENDYDFIAASFVRCADDVLAIRKI
                     LEEKGGEDIHIIAKIENMQGVQNIDEIIRVSDGIMVARGDMGVEIPLEDVPVIQKMII
                     KKVSGAGKVVITATQMLDSMMKHPRPTRAEATDVANAIYDGTSAIMLSGETAAGQYPI
                     EALKTMVRIAVRTECDINYLQRFKDRKNMSSPDVTNAISHATCTMAGDLNAAAILAVS
                     KSGRTARMISKYRPYSPIIGACLTEKVYRQLGLSWGVAPLLLEQKNKAEELFDYAVDT
                     AETAGLISKGDVVVLTAGVPLGVSGTTNLIKVQVAGHILVKGKGIAGKKIAANLCVCH
                     SEEDLANFKEGDIIVAADTNNRMMEQMRQASGLIVEADSDSCHAAIAGLSLDIPVLIG
                     AKNALEVLKSSAYVELDSENGVVTAI"
     gene            complement(5143..6075)
                     /gene="arcC"
                     /locus_tag="ACZO9R_00985"
     CDS             complement(5143..6075)
                     /gene="arcC"
                     /locus_tag="ACZO9R_00985"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019546 - L-arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HIR0911680.1"
                     /translation="MKKKKIVIALGHDALGTTLPEQKEATKRTAKAVADFIREDYQVV
                     ITHSNGPQVGMIHTAMNEFCRLYPEYTATPMSVCSAMSQGYIGYDLQNAIRAELLNRG
                     IYKTVSTILTQVVVDPYDEAFYKPSKIIGRVMTEEEAEAEEKKGNHVTKVADGYRRIV
                     AAPKPVDIVEMDAIRALTDADQIVVACGGGGIPVLSQDNHLKGASAVIEKDLAAGKLA
                     ELLEADMLVILTSVDNVCLDYGTENERPLSSMTVAEAKKYMEQGQFGEGDMQPKIQAA
                     IDFIGDSAIRSVLITKLHVDGSQVTPGMGTMITK"
     gene            complement(6352..>6935)
                     /gene="yihA"
                     /locus_tag="ACZO9R_00990"
     CDS             complement(6352..>6935)
                     /gene="yihA"
                     /locus_tag="ACZO9R_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HIR0911681.1"
                     /translation="VNLEIVCGITSKIPDTAYNEVAFAGKSNVGKSSLINALMNRKAL
                     ARTSAQPGKTQTINFYNINDAMYLVDLPGYGYAKASEEVKAKWGKMIENYLHTSKRLK
                     AVFLLIDIRHDPSANDRMMYEWMVYQGFAPIIIATKLDKIKRSQIQKNVKAIREGLGV
                     QPGTMIIPFSAETKQGREEIWELIDSLVLPQDE"
BASE COUNT         1555 a         1965 c         1622 g         1793 t
ORIGIN      
        1 ccatttcccc tgtatttaag cgtattttga ttgatttttt tatttttaca gtttatactt
       61 ttaggtagaa attgtttact taatcgtttt cgcaattatt tcagtttgaa caattcactt
      121 acctattttc gttgcaaatg tttcggaaat gcgtatgctt acatgatatg aatggaggta
      181 agcccatgaa attcggctat tttgatgatg cgaagaaaga atacgtcatc acgtctccgc
      241 ggacaccgct tccgtggatc aactatctgg gatcaaagga tttcttttcc ctgatctcca
      301 acacctgcgg cggatacagt ttttataaag acgcaaagct cctgcgtctg accagatacc
      361 gctataacaa cgtcccgttc gacagcaacg gtcattacta ctatattaag gaaggcgata
      421 caatctggaa tcctggatgg atgcccacaa agaccgagct ggattcctat gagtgccatc
      481 acggcatggg atattccacc ttccgcagtt ccaagaatga tctctccgca gagctgaccg
      541 cttttgttcc ggtcgacgac agctgcgaga tcaacaagct cacactcacc aacaacggca
      601 gcgcgccaaa gacgttctcc gttttctcct acgtggaatt ctgcctctgg aacgccatgg
      661 atgacatgac gaacttccag cgtaattttt ccaccggtga ggtagaggtt cacggttccg
      721 caatttacca caagacggaa taccgcgagc gccgcaatca ctacgcgctc tatgcggtca
      781 acgctccgat cgcaggtttt gacaccgacc gtgattcctt cctcggcgca tacggagaga
      841 attccgctcc cgaggtggtt gtcaccggtg cttccaagga ttccatggca agcggctggg
      901 ctccggtcgg ctcgcatcac ctttccgtgt cgctggcacc gggcgaatct aaaactttcg
      961 tgttcgttct cgcctacatt gagaacccca tcgaccaaaa atgggtcgga cgcccggagg
     1021 acggcgtgat caaccgcgca ccggcagaag ccctgatggc gcgctacgac accaccgaga
     1081 aggcggatgc cgcacttgcc gcattaaagg aatactggga caaactgctc tcccacttca
     1141 ccatctcttc ctccgagaag aagctcgacc gcatggtgaa cgtatggcac cagtatcagt
     1201 gtatggttac cttcaacatg agccgttccg cttcctattt tgagtccggc atcggacgcg
     1261 gcatgggatt tcgtgattcc tgtcaggatc tgctcggttt cgttcatctg atcccggatc
     1321 gtgccaggga gcgcatcctc gacatcgccg caacacagtt tgaggacgga agcgcatacc
     1381 atcagtacca gccgctcacc aagaagggca actccgacat cggaagcggt ttcaacgatg
     1441 atccgctgtg gctgattgcg ggtgctgccg cctacatcaa ggagaccggc gactacacga
     1501 tcttagatga gatgacgcca tacgacagtg atccttccaa ggcaacagac ttcatggagc
     1561 acctgcgccg ctccttccac tacacgatgg agcacctcgg tccgcacggt ctgccgttaa
     1621 tcggtcgcgc cgactggaat gactgcctga atctgaactg cttctccacc gaacccggag
     1681 aatcgttcca gacgttcggt ccttccgaag gtccgaatgc cgagtccgtc tttatcgccg
     1741 gcatgttcgt gcgctacgga aaagactatg cggcaatctg ccgtcacaga ggcatggatg
     1801 acgaggcagc ggttgcagag aaagcgatcg ccgatatgga gaaaacggtt cttgacgccg
     1861 gctgggacgg tgaatggttc ctgcgcgcct atgaccacta caagaacaaa gtcggttcca
     1921 aggagtgtga agaaggcaag attttcatcg agccacaggg cttctgcgtt atggcagaga
     1981 tcggtctcaa agaaggcaac tgcttaaagg caatgcagtc tgtcgagaag tatctcgaca
     2041 ccaagtacgg cattgttctg ctgcagccgg cttaccacag atatcacgta gaactcggcg
     2101 agatttcttc ctatccgccg ggatacaagg aaaatgcagg tatcttctgt cacaacaatc
     2161 cgtggatttc gattgctgag acagtcgtcg gacgcggcaa ccgcgcatgg caggtataca
     2221 ctagaacctg tcctgcctac atcgaggaca tcagtgaagt ccaccgcacc gagccatatg
     2281 tctactcaca gatgatcgcc ggcaaagatg ccaagaattt cggtgaggcc aagaacagct
     2341 ggctgaccgg aacggctgca tggacgttcc ttgacgtctc ccagtacatt ctgggcatcc
     2401 gcccggatta tgacggactg gtgatcgatc cgtgtattcc gtcctctctg gatggcttta
     2461 ccgccagaag agatttccgc ggtacgactt accacgtgac cgtcaagaac ccgaaccatg
     2521 tggaaaaggg ggtcatttcc atgaccgtcg acggcaagga tgtcgatgtg tccgggatct
     2581 ccggcggcat gattccgatc gcactcgcgg acggaaagaa ggacgtgaat gtcgaggttg
     2641 taatgggata acccgctgag gcttttgtca caaagaattg tcgcaataaa catagaccgc
     2701 tttgggagtg ataagtaata ttataagaaa tagatactta aaagttccag ataaacatgc
     2761 gacggcaaat tctgaccgca aaatccggta tttctaacca tgtcgccgct ggcatctgtg
     2821 atgatccgtg tctttccaag tttggaacac tctatggact gtgtccgcaa gccggcagac
     2881 aattttagga cttttgtgtg tgtcccttta tggcacctct gaacaaaaca taatcgcgcg
     2941 aagccgatgt ttgaattgcc atctgccata tttttaatag cagatggcaa cgagtttcgg
     3001 ttcgcgcggt tttatattgt ctgtgaagag gtgccattag ggacaccaga aagtccgctc
     3061 attgtctgcg gcttgtcgga cacactccgt gagtgtccaa acgtggccaa gacacggaac
     3121 gggatgccag ctgcggcatg gaagaatacg tgaattttgc ttcaaagaat ttgccgccgt
     3181 ctgtttattc tggaactttt tattctctag tatccctgta atattacttg tcacccaaaa
     3241 gggggctatg cttattgtga cagtttcttt aaaaaaagcc tcatgcggtc ttcccatgga
     3301 agaaccgcgt gaggcttttt cacatattcg ctatcactta gatcgctgtc acaactccgt
     3361 tttcgctgtc aagctctaca taagcgctgg actttaatac ctccagtgcg ttcttagcgc
     3421 cgatcagtac cggaatatcc agactgagtc ctgcgatcgc cgcatggcag ctgtcgctgt
     3481 ccgcctccac gatgagaccg gatgcctgac gcatctgctc catcatgcgg ttgttggtat
     3541 cggctgcaac gatgatgtct ccctctttga agttcgcaag atcttcctcg ctgtggcaga
     3601 cgcacaggtt tgctgcaatc ttcttacctg caattccttt tccctttacc agaatatgtc
     3661 cggctacctg taccttgatg aggttggtcg ttccggatac gccgagcggt acgccggcag
     3721 tcagaacaac cacgtcgccc ttgctgatca gacctgccgt ctctgcagtg tcaaccgcat
     3781 agtcaaacag ttcttccgcc ttattcttct gctcaagaag cagcggtgca acgccccagg
     3841 aaagtccgag ctgacggtat actttctccg tcaggcacgc gccgatgatc ggagagtacg
     3901 ggcggtactt ggagatcatg cgcgctgtgc gtccggactt ggatactgcc agaatcgccg
     3961 ccgcattgag gtctcccgcc atcgtacagg ttgcatggga aatcgcgttc gtcacatccg
     4021 ggctgctcat attcttacgg tccttgaaac gctgcagata gttgatatcg cactcggtgc
     4081 gaaccgcaat gcggaccatc gtcttcaatg cctcgatcgg atactggccg gcagccgtct
     4141 caccggaaag catgatcgca ctcgttccgt catagattgc gttggcaacg tctgtcgcct
     4201 ccgcgcgggt cggacgcgga tgtttcatca tggaatccaa catctgggtc gccgtgataa
     4261 ccacttttcc ggcaccgctg actttcttga taatcatctt ctgaatgaca ggaacgtcct
     4321 caagcggaat ctctacaccc atgtcgccac gggcaaccat gataccgtcg gagacgcgga
     4381 tgatctcatc gatgttctgc acaccctgca tgttctcgat cttcgcgatg atatggatgt
     4441 cctcgccgcc tttttcctca agaatcttgc ggattgcaag cacgtcatcc gcgcagcgca
     4501 cgaaggatgc cgcaatgaaa tcatagtcgt tctccaccgc aaatacaata tcgccgtagt
     4561 ctttctcgct gatgtacggc atggaaagct ccacgttcgg aacattcacg ccctttttat
     4621 tggaaaccat accgccgttc ataacggtac agacaatatc catcggcatt ttgccctctg
     4681 catccgcctt tgtgcccgat actttctgga tcttcttgac ttccagaccg atcagtccgt
     4741 catcgatcag aatggtatct cccggcttca catcgtccgg cagatccttg tacgtgatcg
     4801 ccacacggct ctcatcgccc acgatatcct ccgtcgtcag agtaaatgtc tgtccgttct
     4861 tcagctccac cttgccgctt gcgaactctt tcagacggat ctccggcccc tttgtatcca
     4921 gtagagccgc caccggacgc ttcatttcct ctcgcagctt gcgaagcatc gtaagtcttc
     4981 caaactgctc ctcgtgtcct ccatgtgaaa agttaaagcg cgccacattc atgccttcct
     5041 cgatcaggct gcgcatcaca ttctcatcat ccgtcgacgg tcctaacgta catacaattt
     5101 ttgtctttct taacataata ttcctctctt tattctgtca ctttattttg taatcatggt
     5161 gcccatgccc ggagttacct ggcttccatc cacatgaagc tttgtaatca atacggagcg
     5221 gatcgccgaa tcaccgataa agtcgatcgc agcctgaatc ttcggctgca tatccccctc
     5281 gccgaactgt ccctgctcca tatatttctt tgcttccgcc actgtcatgg aactcagcgg
     5341 gcgctcattc tctgtgccgt agtccaggca cacattgtct acgcttgtca gtatcacgag
     5401 catatctgcc tccagaagct ctgcaagctt tccggctgcc aggtccttct cgatcactgc
     5461 acttgctccc tttaaatgat tgtcctggga aagaaccggt ataccgcctc ctccgcaggc
     5521 aacaacgatc tgatccgcat ctgtcagtgc gcggatcgca tccatctcca caatatcgac
     5581 cggcttcggc gctgccacga tacggcggta gccgtctgcc accttcgtca catggttgcc
     5641 cttcttctcc tctgcttctg cctcttcctc cgtcatcaca cggccgatga tcttgcttgg
     5701 cttatagaat gcctcatcgt acggatcaac caccacctgt gtcaggatcg tggatacggt
     5761 cttgtagata cctctgttca gaagctctgc acgaatcgca ttctgcagat catatccgat
     5821 atatccctga ctcatcgccg agcagacaga catcggcgtc gccgtatatt ccggatacag
     5881 acgacagaac tcattcatcg cagtgtgaat catgccgacc tgcgggccgt tgctgtgcgt
     5941 gatgaccacc tgatagtcct cacggataaa atcagcgacc gcctttgccg tgcgcttcgt
     6001 agcttccttc tgctccggca gtgtcgttcc cagtgcgtca tgtcccagtg caataacgat
     6061 cttctttttc ttcattttta tcatcctcca acgcttttat gcgcgcattt gtaatccctt
     6121 ttccctgtat acaccaaaaa attttaccag ttttatttta tcacgaaccc tctgtcatgc
     6181 gcaatgggca acttgtcacg gaagcgttaa agtttgccgc accagaacgc aaaaagcggg
     6241 aaccgcacac acgttttttg tatgtgcgat tcccgccctg tgttttctaa tctcccgttt
     6301 ttccggctgg aattcaccgg aattcgtcct gcccaaactt ctgcccggct cctactcgtc
     6361 ctgcggcagg acaagcgaat cgatcaattc ccagatctcc tctctaccct gcttggtctc
     6421 cgccgagaac ggtatgatca tcgttcccgg ctgcacgccg agtccttcac ggattgcctt
     6481 gacatttttc tgaatctggc ttcgcttgat cttgtccagc ttcgtcgcaa tgatgatcgg
     6541 ggcaaatccc tgatacacca tccactcata catcatcctg tcgttggcag acggatcatg
     6601 ccggatatca atcaagagaa aaacagcctt cagacgtttg gaggtgtgaa gatagttctc
     6661 gatcattttc ccccactttg ccttcacttc ctcggatgcc ttggcgtaac cgtaacctgg
     6721 aagatccacc agatacatgg catcgttgat attgtagaaa ttgatcgtct gtgtcttgcc
     6781 cggctgtgcg gaggtacgcg ccagcgcttt ccggttcatc agcgcattga tcagcgatga
     6841 ttttccgacg ttcgactttc ccgcaaatgc cacttcattg taagccgtat ccggtatctt
     6901 gctcgtaatt ccgcagacaa tttcaagatt tacat
//
LOCUS       DBMDQJ010000026         6846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4600_length_6846_cov_1.439140, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000026 DBMDQJ010000000
VERSION     DBMDQJ010000026.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6846
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4600_length_6846_cov_1.439140"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..74
                     /locus_tag="ACZO9R_00995"
     tRNA            1..74
                     /locus_tag="ACZO9R_00995"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Val,seq:cac)
     gene            269..748
                     /locus_tag="ACZO9R_01000"
     CDS             269..748
                     /locus_tag="ACZO9R_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HIR0911682.1"
                     /translation="MSQKQVAGWLKGITLSIAGMGLLFFGLVVPYLAWDMAVTYPDYA
                     ALRIPGILYGWGIAVFCYAILYQFWKVCVQIGRDNSFSGENARSFVVISRLAFVLAVV
                     WFAGILFLAAKRALGPAFLIFMILLIFINVVIAVLAAALSHLIYKSYEMKQDAELTI"
     gene            783..1028
                     /locus_tag="ACZO9R_01005"
     CDS             783..1028
                     /locus_tag="ACZO9R_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0911683.1"
                     /translation="MAIRINIDVMLAKRKMSVTELSEKVGITMANISILKNGKAKAIR
                     MDTLDKICRSLGCQPGDILEYVEEEGEENGDGRDHDR"
     gene            1006..2514
                     /locus_tag="ACZO9R_01010"
     CDS             1006..2514
                     /locus_tag="ACZO9R_01010"
                     /inference="COORDINATES: protein motif:HMM:NF025067.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR0911684.1"
                     /translation="MEEIMIGREMNPKEMIPEEENQEGAKIFGKLAGASLLYALVYTG
                     CLFENVMGLASVLWVASSVWYVRFVFRMFGEKERRDNGIVLGMIVLLGISTVLTGNEW
                     IGWMNDLIIFVLLVGILLHNFAQDTGWNFGKYLAEIVAAVCGAVCFIGKPFADGGAFY
                     RSKKTRESHAGWYVLIGICAAVPCLMVLVLLLMSADMVFADLLVRFAGVFRFPVKLLR
                     ILLMLVFGFLSSYCGVRYVQKHAADIKPSAGKTAEPLIAIAFTAPIAVLYLLFSAIQI
                     VYLFIGNMQLPAGVTYAEYARRGFFQLLFVCVLNLAAVLSIQSYFKENRVLKALLLAI
                     SGCTLIMTASSACRMLLYIRAYQLTFLRVSVLVALAVIALLMAGVIAKIVKPQFPLFR
                     YGFVLAGAVYLVFAFSHVDYFIAAYNLTHTSWEASGEETVDYSYLYTLSTDAAPAIEK
                     YAGKSGAADYRKQGWYQLYCEANEEALNETGLRTYNVSHAVAKQLLMEDNRE"
     gene            2564..3565
                     /locus_tag="ACZO9R_01015"
     CDS             2564..3565
                     /locus_tag="ACZO9R_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HIR0911685.1"
                     /translation="MEEQMARRLVAAEGKKLQQAGLVARTWGNISCRIDENHFVITPS
                     GLDYETMTARDLVLMDIRNGVCCGAHKPSSEKGVHLAAYQTFPEVGYVIHTHQTYATA
                     IGLCGFEQLAMTEEEAEKLGGIARAAYGLSSTEKLAGAVYDAMQSGAKVVFMVHHGVL
                     ICGKDREEAFSRAMLLEEICKRSILGQPKKKPRKDQKRQEKLFHVLQKHFHYVGICDT
                     PAVLLCAASGRGIPAQVDDMAQMIGRKIPCCLHVRRDMLGLLRKYGAVLVPRVGVVVS
                     AGTADDVEALKTLVAKAAVCCLHVRATGASATLSPVNVAIQHHHYVKKYALQKDGEA"
     gene            3582..4904
                     /locus_tag="ACZO9R_01020"
     CDS             3582..4904
                     /locus_tag="ACZO9R_01020"
                     /inference="COORDINATES: protein motif:HMM:NF051259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR0911686.1"
                     /translation="MNGKHAIYRVYQALCAGMLSLFPAGRQTLVQGDGCITRIPELLK
                     THGIKGVQVITTPGTIRRGTIAPLLAQCEDAGIRTVLYTGVSPDPTVAEVDAAAECFF
                     VSGCEAILAIGGGSVIDCAKVAGARAVKRDLPVGKMSGMLKIHKKLPLLLAVPTTAGT
                     GSEVTLAAVITDETCHYKHAVMDYCLIPRYAFLDPGLSCSLPPDMTAYSGMDAMTHAV
                     EAYCNRFCSPKMKAHAMKAVSLIGANLLTAYREPANKAARMNMLTGSYEAGIAFTNAY
                     VGYVHAIAHGIGGLYHVPHGEANAILLPKVLAAYGSAVYEPLARLERKVRAAWGKPER
                     EVRMSGAEPEREVRMSGAGMERKVCASGAEDGNSKETDAVLAEAFIERIRRMNRSMGI
                     PEQLAMLRKEDVPELAARALKEGNPNYPVPVIWEEEQMRAFLMEQVPK"
     gene            5087..6535
                     /locus_tag="ACZO9R_01025"
     CDS             5087..6535
                     /locus_tag="ACZO9R_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR0911687.1"
                     /translation="MYADENVIKIKHDVLYEVAKLAFAGELDEKRDNIAMSLIPGPTP
                     QFRCCIYKEREIIRQRVRLAEGKAPGLTDDGNVIQVISSACEDCPISSYIVTENCQNC
                     LGKACINACKFGAIEPGRTRSHIDPTKCKECGRCAQACPYNAIAHLKRPCKFSCPVNA
                     ITYDEHGISVIDKKKCIRCGKCIHSCPFGAIGSKSFIVDVINALREGKHVYAMAAPAT
                     EGQFGDDITMASWKNAMKELGFTDFFDVGLGGDMTAAYESEEWAEAYKAGEKKVTSCC
                     PAFVNMVRLHYPDLADCVSTTVSPMCAVSRLIKARDPEAITVFIGPCIAKKSEVVDQQ
                     IPGNADYVLTYSEIRAIMKAKSVVLQPAANDDQIASTFGKRFANSGGVTAAVLQSLKE
                     SEDEIDAKVCVANGAAECKKALLLLKAARLPEDFIEGMACEGGCVGGPSAFNDQISSK
                     KNRDTLISQADDRGIHENIEHYEMNTISMHRE"
     gene            6718..>6846
                     /locus_tag="ACZO9R_01030"
     CDS             6718..>6846
                     /locus_tag="ACZO9R_01030"
                     /inference="COORDINATES: protein motif:HMM:NF019887.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HIR0911688.1"
                     /translation="MKKAVFFDIDGTLWNEHMQVPESTTEAIRELRADGNYAFLCSG"
BASE COUNT         1665 a         1487 c         2162 g         1532 t
ORIGIN      
        1 ggaagcatag ctcagccggg atgagcgttc gcctcacacg cgagaggtca cgggttcgag
       61 ccccgttgct tccactggag agtaccgaga gatcggtgct cttttttgcg taatgggaaa
      121 caccgttcct ggtatttttt cttaagattc ctttaaaacg atatgaattt atcgaaaaac
      181 gataaaaata tattgcaaaa tggaaaaaca tggatataat aaagctgtga gaaaaataca
      241 gcagtaagat tgcggaaaag aggtaattat gagtcagaaa caggtggcag gttggttaaa
      301 ggggatcaca ctttcgattg cagggatggg actgttgttc ttcgggctgg tggtgccgta
      361 tctggcatgg gacatggcgg tgacatatcc ggactacgcg gcgctccgca ttccggggat
      421 tctctacggg tggggcattg cggtgttctg ctatgccatt ctctatcagt tctggaaggt
      481 ctgcgtgcag atcggacgcg acaattcttt ttccggtgag aatgcaaggt cgtttgtggt
      541 aatcagcagg ctggcgtttg tgctggcggt ggtctggttt gcaggaattc tgttcctcgc
      601 ggcaaagcgg gcgcttggac cggcatttct gatttttatg attctgctca tttttatcaa
      661 tgtggtgatc gcggtgcttg cggcggcgct gtcccatctg atctataagt cgtatgagat
      721 gaagcaggat gcggagttga caatatagga cggcagcgga tcgtccggaa atgaggaaga
      781 gaatggcaat caggatcaat atagatgtca tgctggcgaa gcggaaaatg agcgtgactg
      841 agttatcgga aaaagtcggc attacaatgg cgaatatttc gattttaaag aacggaaagg
      901 caaaggcgat ccggatggat acgcttgata agatctgccg gtcattgggc tgtcagccgg
      961 gagatattct ggagtatgta gaggaagaag gggaggagaa cggcgatgga agagatcatg
     1021 atcggtaggg aaatgaatcc gaaggaaatg attccggagg aagaaaatca ggaaggggca
     1081 aagattttcg ggaagctggc gggcgccagc ctgctctatg cgcttgtata cacgggatgc
     1141 ctgtttgaaa atgtgatggg actggcgtcg gtgctttggg tggcgtcgag tgtgtggtat
     1201 gtacgttttg tgttccgaat gttcggggaa aaggagagac gggacaatgg cattgtgctg
     1261 gggatgattg tactgcttgg aatctctacc gttctgacgg gaaacgaatg gatcggctgg
     1321 atgaatgatc ttattatttt cgtgctgctt gtggggattc ttctgcacaa cttcgcgcag
     1381 gataccgggt ggaatttcgg aaaatatctg gcggagattg tggcggcggt gtgcggggcg
     1441 gtctgcttta tcgggaagcc gtttgcggat ggcggagcat tttaccgcag caaaaagaca
     1501 agggagagcc atgcgggctg gtatgttctc atcgggatct gcgcggcggt gccctgtctg
     1561 atggttctgg tgctcctgtt aatgtctgcg gacatggtat ttgcagattt gctcgtacgg
     1621 tttgcgggag tgttccggtt cccggtgaag ctacttcgga ttctgctcat gctggtgttt
     1681 ggattcttat cctcctactg cggtgtgcgg tatgtacaaa agcacgcggc agacattaag
     1741 ccttccgccg gaaaaacggc ggaaccgctg atagcgattg cgtttaccgc gccgattgcc
     1801 gtgctgtatc ttctgttttc ggcaatccag atcgtgtatc tttttatcgg aaacatgcag
     1861 ctcccggcgg gggttactta cgcggagtac gccagacgcg ggtttttcca gcttttgttc
     1921 gtgtgcgtgc tgaatctggc tgcggtgctt tccattcaga gctattttaa ggagaacaga
     1981 gtattgaagg cgctgctcct tgccatttca ggctgcacgc ttattatgac ggcgtcgagc
     2041 gcctgccgga tgcttctcta tatccgggcg taccaactga cgtttctgcg tgtgtcggtg
     2101 ctggtggcac tggcggtaat tgcacttttg atggctggcg tgatcgcaaa gattgtaaaa
     2161 ccgcagttcc cgctgttccg gtatggattt gtgctggcgg gagcggtgta tctcgtattt
     2221 gccttttccc atgtggatta ttttatcgcc gcctacaatc tgacgcacac ctcgtgggaa
     2281 gcgtccgggg aggagacggt ggactacagc tatctctaca cactctcgac ggatgcggcg
     2341 ccggcgattg aaaaatatgc gggaaaaagc ggcgcggcgg actacagaaa gcagggctgg
     2401 tatcagctgt actgcgaggc gaatgaagag gcattaaatg agacgggact tcggacatat
     2461 aatgtttcac atgcggttgc aaaacagctt ctgatggaag ataatagaga atagagaaaa
     2521 agccgggtga cagcgggcac cggcagaagc ggaggagact ggaatggaag aacagatggc
     2581 gagacgcctt gtcgcagcgg agggaaaaaa gctgcagcag gcgggactgg tggcgagaac
     2641 ctggggcaat atcagctgcc gtatcgacga gaatcatttt gttatcacgc cgagcgggct
     2701 ggattatgag acgatgacgg cgcgggatct ggtgctgatg gacataagaa acggtgtgtg
     2761 ctgcggcgcg cataagcctt cgagcgaaaa gggtgtgcat ctggcggcgt atcagacatt
     2821 cccggaggtg gggtatgtca ttcacaccca tcagacctat gccacggcga tcgggctctg
     2881 cggctttgag cagcttgcca tgacggagga agaggcagag aagctcggcg ggatcgcgag
     2941 agcggcatac gggctttcca gtacggagaa gcttgcaggg gcggtctacg atgccatgca
     3001 gtcaggagca aaggtggtct ttatggtgca ccacggcgtg ctgatctgcg gaaaggacag
     3061 ggaggaggcg ttttcccgcg ccatgctctt agaggagatc tgtaagcgga gcattttggg
     3121 acagccgaaa aagaagccga gaaaagacca gaagcgccag gaaaagctgt ttcacgtctt
     3181 acagaagcat tttcattatg tgggaatctg cgatacgccg gcagtccttc tctgcgcggc
     3241 gagcgggcgg ggaatcccgg cacaggtgga tgatatggca cagatgatcg gcaggaagat
     3301 tccgtgctgc ctgcatgtgc gcagggacat gctcggactc ttaagaaaat acggcgcggt
     3361 gcttgtgccg cgggtcggcg ttgtggtatc cgcgggaacg gcggatgatg tggaagcgtt
     3421 aaagacgctt gtggcaaagg cggcggtctg ctgtctgcat gtgcgcgcga cgggcgcgtc
     3481 ggcaacgctc tctccggtca atgtggcgat ccagcatcat cattatgtga aaaagtatgc
     3541 cctgcagaaa gatggggaag cataagggca ggaggaataa gatgaacgga aaacatgcca
     3601 tataccgcgt ctatcaggcg ctctgcgcgg ggatgctttc cctgtttcct gcgggaagac
     3661 agacgctggt gcagggggac gggtgtatca ccaggatacc ggaattattg aaaacacacg
     3721 ggataaaagg ggtgcaggtc atcaccacgc cgggaacgat ccggcgcggg acgatcgcgc
     3781 cgcttctggc gcagtgtgag gatgccggca tccggacggt gttatacaca ggtgtatcgc
     3841 ctgatccgac cgtggcggag gtggatgcgg cagcggaatg tttttttgta tccggctgtg
     3901 aggcgatcct tgccatcgga ggcggctctg tcatagactg cgccaaggtc gcgggagcgc
     3961 gcgctgtaaa gagagatctc ccggtcggaa aaatgagcgg gatgctcaag attcacaaaa
     4021 agctgccgct ccttcttgca gtgccgacga cggcgggaac cggttcggag gtgacgctgg
     4081 cggctgtcat cacggacgag acatgccact ataagcatgc cgtcatggat tactgtctca
     4141 taccgcgcta cgcatttctc gatccgggac ttagctgttc cctgccgccg gatatgaccg
     4201 cctatagtgg gatggatgcg atgacgcacg cggtggaggc gtactgcaac cggttctgtt
     4261 ccccgaagat gaaagcgcat gccatgaagg cggtgagcct gattggtgcc aatctgctga
     4321 cggcttaccg cgagcctgcg aacaaggcgg cgcggatgaa tatgctgacc ggttcctacg
     4381 aggcggggat cgcgtttacc aatgcctatg tcgggtacgt gcatgcgatt gcacatggga
     4441 tcggcggact ctaccatgtc ccgcacgggg aagcgaatgc gattctgctg ccgaaggtgc
     4501 ttgcggcata cgggagtgcc gtgtatgagc cgctggccag gctggagagg aaagtgcgcg
     4561 cagcctgggg gaagccggag agggaagtgc gcatgtccgg agcagagccc gagagggaag
     4621 tgcgaatgtc cggagcagga atggagagga aagtgtgcgc atccggggcg gaggatggaa
     4681 acagcaagga gacggacgcc gtgctggcgg aggcgtttat tgagagaatc cgccggatga
     4741 accggagcat gggcatcccg gagcagcttg ccatgctgcg caaggaggac gtgccggaac
     4801 ttgcggcgcg cgcgctcaaa gagggcaatc cgaactaccc cgttcccgtg atctgggaag
     4861 aggagcagat gcgggcgttc ctgatggaac aggtaccaaa gtaacagtac agtggacttc
     4921 ccagaaaaat attgcacaaa acagcagtcg gattttttac aatttgacgg tatacatcat
     4981 gtacgcaaag aaatacatgc gtttctcttg caattccata cagataatat ataatgtcta
     5041 agatggttaa aaaattaacg aatagaaaag ggcgaggtaa aacacgatgt atgctgatga
     5101 aaacgtgatc aaaatcaaac acgacgtact ctatgaagtg gcaaagctgg catttgccgg
     5161 tgagcttgac gagaagagag ataatatcgc aatgagcctg atcccaggtc cgacaccgca
     5221 gtttcgctgc tgtatttata aagaaagaga gattatcaga cagagagtcc gtctggcgga
     5281 gggaaaagcg ccaggcctga ccgatgacgg taatgtgatc caggtcatca gttctgcatg
     5341 tgaggactgt ccgatttcca gttatattgt aacggagaac tgccagaact gtcttggaaa
     5401 ggcttgtatc aatgcctgca aattcggtgc gattgagccg ggaagaaccc gctcccacat
     5461 cgatcctaca aaatgcaaag aatgtggaag atgtgcgcag gcctgtccgt acaatgcaat
     5521 cgcacatctg aagagaccgt gtaagttcag ctgcccggtc aacgcgatta catacgatga
     5581 gcacgggatc tccgtcatcg ataagaagaa atgtatccgc tgcggtaaat gtatccacag
     5641 ctgtccgttc ggagcgatcg gctccaagag ctttatcgta gatgtaatca acgcactgcg
     5701 cgagggcaag catgtctatg cgatggctgc accggcgacc gaaggacagt tcggggacga
     5761 catcacgatg gcaagctgga agaatgcgat gaaagaactc ggatttaccg atttcttcga
     5821 cgtaggtctc ggcggtgata tgacggcggc ttacgagtcc gaggagtggg cggaagctta
     5881 caaggcgggc gagaagaaag ttacctcctg ctgtccggca ttcgtaaata tggtacgcct
     5941 gcactatccg gatctggcag actgtgtatc gacgacggta tctccgatgt gcgcggtttc
     6001 ccgcctcatc aaggcaagag atccggaagc catcacggta tttatcggac cgtgtatcgc
     6061 aaagaagtct gaggttgtgg atcagcagat tccgggcaat gcagactatg tactgaccta
     6121 cagtgagatc cgtgcgatta tgaaagcgaa gagcgtcgta ctgcagccgg ctgcaaacga
     6181 tgatcagatc gcatccacct tcggtaagcg ttttgcaaat tccggcggtg taaccgcagc
     6241 tgttcttcag agtctgaaag agtctgagga tgagatcgat gcaaaggtat gcgtggcaaa
     6301 cggagctgcc gagtgtaaga aagcactgct tcttctgaaa gcagccagac tgccggagga
     6361 tttcatcgag ggtatggcat gcgagggcgg ctgtgtcggc ggaccgagtg cgttcaatga
     6421 ccagatctct tccaagaaga accgtgacac tctgatttcc caggcagatg accgtggaat
     6481 tcatgagaac attgagcatt acgagatgaa caccatctcc atgcacagag agtaacagga
     6541 tatttgaaat agaaaccgca agttgccaga caccgcgcgg gctgatataa tgagttcagc
     6601 ccgcgcggtg tttttttcct ttataggatg ggatgtgcac catgcggtag ggaagagaat
     6661 gttttgcata tagcagaact gacttttacg caagagaatg ggaattggag agaaaaaatg
     6721 aaaaaagcag tgttttttga tatagacgga actttgtgga atgagcacat gcaggtgccg
     6781 gaaagtacaa cggaagcgat acgcgaactt agggctgacg ggaattacgc gtttttgtgc
     6841 agcggc
//
LOCUS       DBMDQJ010000027         6780 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4658_length_6780_cov_1.416380, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000027 DBMDQJ010000000
VERSION     DBMDQJ010000027.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4658_length_6780_cov_1.416380"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /gene="miaA"
                     /locus_tag="ACZO9R_01035"
     CDS             complement(<1..594)
                     /gene="miaA"
                     /locus_tag="ACZO9R_01035"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR0911689.1"
                     /translation="MKPNPMVILTGPTAVGKTALSIALAKKINGAIISADSMQVYKYM
                     DIGSAKIMPEEMDGVRHYLVDELDPSDEFHVVRFVEMAKQALKEITANGQIPIVVGGT
                     GFYIQALLYDIDFTEQECDSAYRRQLEELAETRGNHYLHEMLKAVDPASAEAIHENNR
                     KRVIRALEFYHESGERISEHNERESQKTSPYNFAYFVL"
     gene            complement(597..2699)
                     /gene="mutL"
                     /locus_tag="ACZO9R_01040"
     CDS             complement(597..2699)
                     /gene="mutL"
                     /locus_tag="ACZO9R_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR0911690.1"
                     /translation="MPQITLLSQETIDKIAAGEVVERPSSVVKELVENAIDARATAVT
                     VEIKEGGTTFVRITDNGCGIEREQVPLAFLRHSTSKIKSVEDLLCIHSLGFRGEALSS
                     IAAVSQVELITKTYSDLTGTRYVIEGSREMSNDEIGAPDGTTFIVRNLFYNTPARKKF
                     LKTAQTEGNYISDLMERLALSHPDVSFKFISNGQTKMHTSGNSNEKDLIYHIYGRDIT
                     AALLPVCAETEYFSVKGFVGKPMISRGNRSYESYFINGRYIKSALLSKAVEEAYKGFM
                     MQHQYPFCVLYFTMDTELLDVNVHPTKMELRFSNNEEVYRKLYQTIRDVLTHKEFIPA
                     VPVEEKKEEKRPAITGSLPEPFETKRLHIPPQTQVLPQGQNVTPEPVRTPENLDVFRQ
                     LLVREESSYHTDSASAGSASTEDGSRMQEMPREAESIPAADTVQPDVRPEPIPKAEVV
                     QGTPDTPENLNTPENLNMPETDTQNVQETQNPQETADTVVYEQQTLEAADTGFFTPDA
                     ARRHRIIGEVFDTYWLIEYEDKLFIIDQHAAHEKVLYERTMARVRTQNFASQTLSPPL
                     ILTLSAEEQEMLTRYGEQIRLFGYEVEPFGGKEFAITAIPADFEAVDMKGMFLDMLDD
                     FTNISGREAPELILEKVASMSCKAAIKGGDSISRAEAEQLIDELLSLENPYHCPHGRP
                     TIISMSKYEIEKKFKRIV"
     gene            complement(2739..>5344)
                     /gene="mutS"
                     /locus_tag="ACZO9R_01045"
     CDS             complement(2739..>5344)
                     /gene="mutS"
                     /locus_tag="ACZO9R_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR0911691.1"
                     /translation="TKEEYKDCILFYRLGDFYEMFFEDAELVSKELELTLTGKNCGME
                     ERAPMCGIPYHAVEGYLNKLVANGHKVAICEQVEDPKLAKGLVRREVIRIVTPGTNLD
                     MQALDESKNNYIMCIVYLADRYGVSIADVSTGDYYVTEVDSERKLFDEITKFSPSEII
                     CNESFYMSGVDIEDLRHRLKITIYALETWYFGDELAETTLLTHFKVKSLEALGLADYD
                     CGMIAAGALLKYLYETQKNDLNNISVIHPYSTGKYMIIDSSTRRNLELVETLREKQKR
                     GSLLWVLDKTKTAMGARLLRSYVEQPLIDKAEIEKRQDAICELNQHVITREELREYLN
                     PIYDLERLITRVTYLTANPRDLIAFRSSIAMLPPIKSLLGDFQCELLGEIREDMDTLE
                     ELCALIDRAIMEEPPISVRDGGLIKDGYNEDVDKYRKAKTEGKTWLAELEAKEREKTG
                     IKNLKIKYNKVFGYYLEVTNSYKDLVPDYFMRKQTLANAERYITPELKELEDMILGAE
                     DKLVTLEYDLFCEVRSTIAAEVVRIQRTAKAVAGLDVFVSLALVADQNNYCRPKINEN
                     GIIDIKGGRHPVVEKMINNDMFIDNDTYLDNGNHRISIITGPNMAGKSTYMRQTALIV
                     LMAQLGSYVPAASAKIGIVDRIFTRVGASDDLASGQSTFMVEMNEVANILRNATSNSL
                     LILDEIGRGTSTYDGLSIAWAVVEHISNPKLLGAKTLFATHYHELTELEGKLNNVNNY
                     CVAVKEKGDDIVFLRKIVKGGADKSYGIQVAKLAGVPDSVIARAKEIVEELSANDITE
                     VARNISVDTGSKKKKEKLDEVDLTQMSLFDTVRDDDIIEELRNVDVGNLTPIEALNKL
                     YELQNKVKNRW"
     assembly_gap    5345..5354
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5378..>6780)
                     /locus_tag="ACZO9R_01050"
     CDS             complement(5378..>6780)
                     /locus_tag="ACZO9R_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0911692.1"
                     /translation="IIKRANDPNVTIVKQTGYHLPGAVSPTPSIPPVIVGAGPAGLFC
                     AYALTMSGCAPVVLERGRSVTERTKDVERFWESGVLDTVSNVQFGEGGAGTFSDGKLN
                     TLVKDPTGRNRFVLETFVKFGAPEQILYENKPHIGTDILAGVIKNMRQTMEARGARFC
                     FESCMTDYRREADGKMFVEVNHKEWIETSRLVLAIGHSARDTFGMLYQKQDLVMEAKA
                     FAVGFRVEHPQEMMNLQQYGALHAHELPAAPYKVTANLANGRGVYSFCMCPGGYVVNA
                     SSEEGRLAVNGMSYSGRDGKNANSAIIVAVTPDDFAGKDPLAGVRFQEMLEERAFAIG
                     KGRIPQQLFGDYCSNRASVSYGAFESATKGACVLGNLRGLLPQALEESFIEGMHAFSR
                     RIPAFDREDAILSGVESRTSSPVRLVRDPKLLVAAGGIYPCGEGAGYAGGIMSAAMDG
                     LKVAEAIMRDMEEQRG"
BASE COUNT         1406 a         2013 c         1603 g         1748 t
ORIGIN      
        1 taagacaaaa taggcaaaat tgtaggggga tgtcttctgg ctctcccgct cgttatgctc
       61 ggagatccgc tcgccggact catggtaaaa ttccagggcg cggatgacgc gcttgcggtt
      121 attctcgtgg atcgcctctg cagatgccgg atctaccgcc tttagcattt cgtgaagata
      181 atgattcccg cgtgtctctg ccagctcttc cagctgcctg cggtatgcac tgtcacactc
      241 ctgctccgta aaatcaatat cgtagagcag cgcctggata tagaatccgg tcccgcccac
      301 cacaatgggg atctgaccgt ttgccgtaat ctcttttaac gcctgctttg ccatctccac
      361 aaaacggacg acatggaact catcggatgg atccagttcg tccacaagat agtgccggac
      421 gccgtccatc tcctccggca tgatttttgc cgagccgata tccatgtatt tatacacctg
      481 catggaatcc gcagatataa tggctccgtt tatttttttc gccagtgcaa tggaaagcgc
      541 tgtcttcccg accgctgtcg ggccggtcag aattaccatg ggatttggtt tcattgtcat
      601 acaatcctct taaacttctt ttcaatctca tatttgctca tggagataat ggtcggccgc
      661 ccgtgcggac aatggtaggg attctccaag gacagcagct cgtcgatcag ctgttctgcc
      721 tccgccctgc tgatggaatc gcctcccttg atcgccgcct tgcaggacat ggaagccacc
      781 ttctccaaga tcagttccgg tgcttccctg ccgcttatgt tggtaaaatc gtccagcatg
      841 tccaagaaca tgcctttcat atcgaccgcc tcaaaatcgg ctggaatcgc cgtgatggca
      901 aattccttgc cgccgaacgg ctccacctca taaccgaaca gacggatctg ctccccgtag
      961 cgtgtaagca tttcctgttc ctcggcgctc aacgtcagaa tgagcggcgg gcttaaggtc
     1021 tgcgaggcaa aattctgtgt gcggacgcgc gccatggtgc gctcatagag cactttttca
     1081 tgagcggcat gctggtcgat aatgaacagc ttatcctcgt attcaatcag ccagtaggtg
     1141 tcaaacactt cgccgatgat ccggtgccgt ctggctgcat ccggcgtgaa aaatccggtg
     1201 tccgccgcct cgagcgtctg ctgctcgtac acgaccgtgt ccgctgtctc ctgcggattc
     1261 tgcgtctcct gcacgttctg tgtgtctgtc tccggcatat tcagattctc cggcgtattc
     1321 agattctccg gtgtatcagg agttccctgc acgacctctg ccttaggaat cggctccggc
     1381 cgcacatccg gctgtacggt atccgccgcc ggtatgcttt ccgcttcccg tggcatctcc
     1441 tgcatccggc tgccatcttc ggtcgaagca gaacccgcag atgcggaatc cgtatgatag
     1501 ctgctctctt cccgcaccag aagctgccgg aacacgtcca gattttccgg tgttctgacc
     1561 ggttccggcg tcacgttctg cccctgcggc agcacctgtg tctgcggcgg gatatgcaga
     1621 cgttttgtct caaatggctc cggcaggcta ccggtaatgg ctggccgctt ttcttctttc
     1681 ttttcctcca ccgggacagc cggaataaat tccttgtgag tcagcacatc gcggatcgtc
     1741 tgatatagct tccggtacac ttcctcattg ttggaaaaac gaagctccat cttggtcggg
     1801 tgcacattga cgtccaaaag ctccgtatcc atggtaaaat acagcacgca gaacggatac
     1861 tgatgctgca tcataaagcc cttgtacgcc tcttccaccg ctttggagag cagtgcgctt
     1921 ttgatgtagc gtccattgat aaaatagctt tcgtagctgc ggtttccgcg ggaaatcatc
     1981 ggttttccga caaagccctt taccgagaaa tattctgtct ctgcgcagac cggaagaagc
     2041 gccgccgtaa tatcgcgccc gtagatatga tagatcagat ctttttcatt ggagttgccg
     2101 gaggtatgca tcttggtctg tccgttgctg ataaacttaa aagagacatc cggatgggac
     2161 aaggcaaggc gctccatcag gtcactgata taatttccct ccgtctgcgc ggtttttaaa
     2221 aactttttcc gtgccggcgt attgtaaaag aggtttctga cgatgaaggt cgtgccgtcc
     2281 ggcgcgccga tctcatcgtt gctcatctcc cttgagcctt caatgacata gcgcgttccg
     2341 gtcagatcgc tgtaagtctt ggtaatcagc tccacctgcg atacggcggc aatgcttgag
     2401 agcgcctcac cgcgaaaccc gagcgaatgg atgcataaaa ggtcttccac actcttgatc
     2461 ttgctggtgg aatggcgcag aaacgcgagc ggtacctgct cacgctcaat tccgcagccg
     2521 ttatccgtaa tgcgcacgaa cgttgtgccg ccctccttga tctcgaccgt gacggcggtt
     2581 gcccgggcat cgatcgcgtt ctcgacaagc tccttgacca ccgaggacgg ccgttccacc
     2641 acctccccgg ctgctatttt atcgattgtt tcctgcgaaa gcagtgttat ctgcggcatt
     2701 ccattcctcc catctgcctt tgccaaaggc gcttccggtt accagcggtt ctttaccttg
     2761 ttctgaagtt cgtataattt gtttaaagcc tcaatcggcg tcagatttcc gacatccaca
     2821 ttccgcagtt cctctatgat gtcatcgtcc cgcacggtat caaacaacga catctgcgtc
     2881 agatccacct cgtccagctt ttcttttttc tttttgcttc ccgtatcgac ggagatattt
     2941 ctcgcaactt ccgtgatgtc gttggcactt aattcctcca cgatctcctt ggcacgcgca
     3001 atgacagaat ccggcacgcc ggcaagcttt gccacctgga taccgtaact cttgtccgcg
     3061 ccgcccttta cgattttgcg gagaaataca atgtcatcgc ccttttcctt taccgccacg
     3121 cagtaattgt tgacgttgtt taatttgcct tccagctctg tcagttcatg ataatgtgtg
     3181 gcaaaaagcg tcttggcacc gaggagcttc gggttgctga tatgctccac caccgcccag
     3241 gcaatgctca gcccgtcgta ggtgcttgtg ccgcgcccga tttcatccaa gatcagcagc
     3301 gagttggagg tagcgttgcg cagaatattg gcaacctcat tcatctccac cataaacgta
     3361 ctctgcccgc tggcaagatc atccgacgct cccacacgtg tgaaaatgcg gtccacgata
     3421 ccgatcttcg cggatgccgc aggcacatag ctgccaagct gcgccatcag aacgatcagc
     3481 gccgtctggc gcatataggt ggattttccc gccatgttcg gtcccgtaat gatcgagatg
     3541 cggtgattgc cgttgtcgag gtacgtgtca ttgtcaataa acatatcgtt gttgatcatc
     3601 ttctcgacca ccggatgacg tccgcccttg atgtcaatga tgccgttctc gttgattttc
     3661 ggacggcagt aattattctg atccgcgaca agtgcgagcg acacgaatac atccagaccg
     3721 gcaaccgcct tggcggtccg ctggatgcgc acaacctccg cagcgatcgt actccgcacc
     3781 tcacagaaga gatcgtactc taatgtgaca agcttgtcct ccgcgccgag aatcatgtcc
     3841 tccagttctt ttaattccgg cgtgatatag cgctcggcgt tggcgagcgt ctgcttgcgc
     3901 ataaaataat ccggaaccag atccttgtag gagttggtca cttccagata atagccgaat
     3961 accttgttat atttgatttt taaattcttg atgcctgtct tttcacgctc tttggcttcc
     4021 agttccgcca gccaggtctt cccttccgtc ttcgccttgc ggtatttatc gacatcctcg
     4081 ttgtagccgt ccttgatcag accgccgtcc cgcaccgaaa tgggcggttc ctccatgata
     4141 gcccggtcga tcagtgcgca gagctcctcc aacgtatcca tgtcctcgcg gatttctccc
     4201 agcagctcgc actgaaaatc gcccaaaaga ctcttgatcg gcggcagcat ggcaatcgaa
     4261 ctgcggaagg caatgagatc cctgggattg gcggtcagat atgtaacacg cgtaatcaga
     4321 cgctccagat cgtagatcgg atttaagtat tcccggagtt cctctctggt aatcacgtgc
     4381 tgattcagct cacagattgc atcctggcgc ttctcgatct ccgccttgtc aatcagcggc
     4441 tgttccacat aggagcggag cagtctggca cccatcgccg tcttcgtctt gtcaagcacc
     4501 cacaggagcg atccgcgttt ctgcttctcg cgcagcgttt ccacaagctc cagattcctt
     4561 ctggtcgagc tgtcgatgat catgtatttt ccggtggaat acggatgaat cacggaaatg
     4621 ttgttgagat cgttcttctg tgtctcgtac agatacttta acagtgctcc cgcagcaatc
     4681 atgccgcagt cataatcggc aagtcccagc gcctccagac tctttacctt aaagtgtgta
     4741 agcagtgttg tctctgcaag ctcatcccca aaataccatg tctccagcgc ataaattgta
     4801 atcttgagtc gatgccttaa atcttctata tcgacaccac tcatataaaa actttcgttg
     4861 catataattt cagatggcga aaattttgtg atctcatcga aaagcttccg ctccgaatcc
     4921 acctcggtga catagtagtc tccggttgag acgtccgcaa tggatacgcc gtagcggtct
     4981 gccagataca cgatgcacat aatataattg ttcttggatt cgtccagtgc ctgcatatcc
     5041 aggttcgttc ccggcgtcac gatccggatg acctcgcgcc tgaccagtcc ctttgccagc
     5101 ttcggatctt ccacctgctc gcagatggca accttgtgtc cgtttgccac aagtttattt
     5161 aagtatccct ccaccgcatg gtacgggatg ccgcacatcg gtgcgcgctc ctccatgccg
     5221 cagttttttc cggtcagcgt cagttccagt tctttggaca ccagttcggc atcctcaaaa
     5281 aacatctcgt agaaatctcc cagccggtaa aacaggatac agtctttata ttcttccttc
     5341 gtctnnnnnn nnnnattgct gcatcattgg tgtaaattca gccacgttgc tcctccatat
     5401 ctctcataat cgcctctgcc actttcagtc cgtccatcgc cgcggacatg atgccgcctg
     5461 catagcccgc gccctcccca cacggataga tgccgcctgc agccacaaga agcttcggat
     5521 cgcgcaccag gcgcaccggt gaggacgtcc ggctctccac gccggagagg atggcgtctt
     5581 cccggtcaaa agccggaatc cggcggctga atgcgtgcat cccctcgata aaggactctt
     5641 ccagtgcctg cggcagcaga cctcttaaat tgccaagcac acatgcgccc tttgtggcgc
     5701 tctcaaacgc cccgtaagac accgaggcac gattgctgca atagtctcca aaaagctgct
     5761 gcggaatccg cccttttccg atagcgaatg cccgctcctc gagcatctcc tgaaaacgga
     5821 caccggctag cggatctttc cccgcaaagt cgtcaggagt taccgccaca ataatcgcac
     5881 tgtttgcgtt tttcccgtca cgtccggaat agctcattcc gttgaccgca agcctgccct
     5941 cctcggagga tgcgtttacc acatatccgc ccggacacat gcagaaggaa tacacgcctc
     6001 tgccgtttgc gaggttcgct gtcactttgt agggggctgc cggaagttcg tgtgcatgca
     6061 gcgctccata ttgctgcaga ttcatcatct cctgcgggtg ttccacgcgg aaaccaaccg
     6121 caaacgcttt tgcctccatg acaagatcct gcttctgata cagcatccca aacgtgtccc
     6181 gcgcactgtg accgattgca agaacgagtc tggaagtctc gatccactcc ttgtggttga
     6241 cctcaacaaa catctttccg tccgcttccc tgcggtaatc tgtcatacaa ctctcaaagc
     6301 aaaacctggc gccgcgcgct tccatcgtct gccgcatgtt tttgatgact ccggcaagaa
     6361 tatccgttcc gatgtgcggt ttgttttcgt acagaatctg ctccggcgct ccgaatttta
     6421 caaaggtttc cagaacaaac cggttcctgc cggtgggatc cttgaccagt gtatttaatt
     6481 tgccgtccga aaaagttcct gcgccgccct ctccgaactg cacgttggaa accgtatcca
     6541 acactccact ctcccagaag cgctccacat cctttgtgcg ctccgtcact gaacgcccgc
     6601 gctcaaggac aacgggtgca cagccgctca tcgtgagcgc atacgcgcag aaaagtcccg
     6661 cagggcccgc accgacaatc accggcggga tggacggcgt tggcgaaacc gccccgggca
     6721 gatgataacc ggtctgcttt acgatcgtaa cattcggatc gtttgccctt tttatgattt
//
LOCUS       DBMDQJ010000028         6685 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4724_length_6685_cov_1.404921, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000028 DBMDQJ010000000
VERSION     DBMDQJ010000028.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6685)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6685)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6685
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4724_length_6685_cov_1.404921"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="ACZO9R_01055"
     CDS             complement(<1..1057)
                     /locus_tag="ACZO9R_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0911693.1"
                     /translation="MDKRLKYYDLMEQLHGKIMSGEIRPGEKLPSENELSAEYGVSRQ
                     TVRKALQILQNEGYIYAEHGRGTFCSEMLRHTTPSKNIAVVTTYLSDYIFPRIVQGID
                     QVLTENGYSILLKNTRNSRSLEAKCLEELLQKDIDGLIIEPSKSQIFCRHVNLYEMLD
                     SYHIPYVFIQGVHAQLLDRPQILLDDCRGAYLATRYLIELGHRRIVGVFKSDDTQGQQ
                     RHKGYVQALQEAGIAYDPDKVIWFYTEDRKTHPFERIRQMAKDRGNHPFEAVVAYNDQ
                     VAIEVIRALNEEGLSVPQDVSVTGYDNSYLAQTCRVPLTTISHPQEELGAAAAELLLR
                     IIREGNSNIEGKCVLMEP"
     gene            complement(1099..1815)
                     /locus_tag="ACZO9R_01060"
     CDS             complement(1099..1815)
                     /locus_tag="ACZO9R_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H1 domain-containing protein"
                     /protein_id="HIR0911694.1"
                     /translation="MAVKYYAVAVGKKPGIYTKWDECKAMVHGFPGAVYKSFKTLEEA
                     ETFLESAPARGGEAHAKGAAQQKATQGAAQQKSGTAGDGKAETRGAESAGETEDLPEN
                     YAFVDGSFHAGTGVYGYGGFLIHAGGKEVLQGAGKEPEMASMRNVAGEVLGSMAAIEK
                     ALELGLKDLTIYYDYMGIEMWAKGLWKRNKKGTVAYYEYVRLVEDKIRLSFVKVKGHS
                     GVMGNEEADRLAKEAVGITG"
     gene            complement(1827..2561)
                     /locus_tag="ACZO9R_01065"
     CDS             complement(1827..2561)
                     /locus_tag="ACZO9R_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HIR0911695.1"
                     /translation="MAVGAVFFLWFFIPAFVVVNIGNITGMAVSALVLLYGIFYRQVQ
                     DVAGRLWQTRGGRAGLSALGMVLLFILLIVTVETAGMIRSAANRAPENTTAVVLGCSV
                     KGERPSRVLSERLCAAYEYLNRNPKAVCVLSGGQGDGEEISEAECMYRYLTGRGIDGA
                     RLLLEDRSTTTAENLQYSMELLQQHGIDGSVTIITSEFHEYRANQTAKRLGITSYSTP
                     SRTFFLYLPTFYVRELYGILYYKISK"
     gene            complement(2662..3012)
                     /locus_tag="ACZO9R_01070"
     CDS             complement(2662..3012)
                     /locus_tag="ACZO9R_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911696.1"
                     /translation="MQDNLREELIKQFPAEVVELVEPSGKKFYACPTCKRVVTLNESK
                     CAGCSQVLSWDHIRQEEMVKGVKTATLKFEVGGDFNKGDCRKCPLSYIAKENNENVYE
                     CPLKMRANCKLDIS"
     gene            complement(3135..3851)
                     /locus_tag="ACZO9R_01075"
     CDS             complement(3135..3851)
                     /locus_tag="ACZO9R_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0911697.1"
                     /translation="MKHAKAVRRIIFTVLIVAAVIVGFNTYTADYYHAKDYELQTTEV
                     SETDAYIAYGDPQSETGLIFYPGAKVEETAYAPVMDGLAQQGIFCVTVKMPAHLAILK
                     PNAADEVRQEFGEVKRWYLAGHSLGGAMAGSYAAKHESELAGLIFLAAYPTRKLQTLP
                     VLSLYGSEDGVLNMEKYQEAIGLAADCREYVLEGGNHAGFGNYGSQKGDGEAGITDRE
                     QWQETIDYIMDMIRQQQGEK"
     gene            complement(3887..>4637)
                     /locus_tag="ACZO9R_01080"
     CDS             complement(3887..>4637)
                     /locus_tag="ACZO9R_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HIR0911698.1"
                     /translation="VAAVFVVLPNTSGSVAYAMGNLPVVGKLVEAVTFRDYQYDNGGH
                     AADIKTPELTVKESDTEQMTDTEVQVQENLKKTTAEINAEIEKITDQIVSEFEESRKE
                     EEGYQEVVVKHEVISTTDDYFTLKLMCYQAAGSGAEWDYYYTIDLKTGERLTLSDLFQ
                     SGADYITPISENIKEQMQQQMAEDDGKMYWVDNAEVPEWNFDKITDETSFYLDSDGHL
                     VICFNEGDVAPMYMGCVQFVIPDEVVADIRK"
     assembly_gap    4638..4647
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4659..4931)
                     /locus_tag="ACZO9R_01085"
     CDS             complement(4659..4931)
                     /locus_tag="ACZO9R_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911699.1"
                     /translation="MAVGIEERMVPHMGNKKQDCKKIEELKRQYAGSEMSGEQVEQMK
                     KRIEEAKMEKRNESRKMHTVRNIVAAASGQGSRCGPGTQHRKTGRF"
     gene            complement(4916..5413)
                     /locus_tag="ACZO9R_01090"
     CDS             complement(4916..5413)
                     /locus_tag="ACZO9R_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0911700.1"
                     /translation="MFFSGKNHKEQKIEQLLLENYNKYYRLAYSYVHHEADAADIVQN
                     GAYKAIKNSDALKQEEYAQTWIYRIMLNEIFGYVKKNREVSLDEMPAEEGREDRYENI
                     DLRRALDAMEERDKAVIELRYFEDMKLEDIARVLGENQNTVKSRLYRGLKKLRLELTD
                     TWQWE"
     gene            complement(5484..6581)
                     /locus_tag="ACZO9R_01095"
     CDS             complement(5484..6581)
                     /locus_tag="ACZO9R_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0911701.1"
                     /translation="MTRYETLAERLESQRHLSTVREHLLFQVARTVRTDVAVIEECYR
                     EMTGQRTENKSVGLEDMYRHLEHIRELTDSVLDGQIVGAGESVCDTFQISDVVAELTS
                     ECRTICTEMGIEVTVQEGEIFCEYLVGDVRRLKQIWARLLKKAYINARPGCRIFTGYR
                     EVRTGEDELELELAVQGSIDGGEDYPLIKSLVELLHGTMCVEVQEDAQILCRVFLPFG
                     IGKAIGQPKSFAGLKTLLLDDNELSRDYHAAVLARLGLPVDVVQNGADAVAYLRRAAS
                     KGNAYDLCFVNWYMQGGEPFIRSVRENFPKGSLTICCSTNEKELVECQMLAAGVDCVV
                     ERPIYQEGMYRFLTELCRQESGKNGTGSEKI"
BASE COUNT         1404 a         2165 c         1325 g         1781 t
ORIGIN      
        1 ccggctccat caggacgcat tttccttcga tattggaatt tccctcccgg ataatccgaa
       61 gcagcagttc ggcggctgcc gctcccagtt cctcctgcgg atgggagatc gtcgtcagcg
      121 gcacgcggca tgtctgcgcc agataggaat tgtcataccc cgtcaccgag acatcctggg
      181 gcacggagag cccctcctca ttcagcgcgc ggatcacctc gatggcaacc tgatcgttgt
      241 acgccaccac cgcctcaaac ggatgattgc cgcgatcttt cgccatctgc ctgatccgct
      301 caaacggatg ggtcttgcgg tcctccgtgt agaaccagat caccttatcc ggatcatacg
      361 caattcccgc ttcctgcagt gcctgcacat accccttgtg gcgctgctgt ccctgcgtgt
      421 cgtcggattt gaagacgccc acaatgcgcc ggtgtcccag ttcaatcagg tatctggtcg
      481 caagataggc tcccctgcag tcgtccagaa gaatctgcgg ccggtccaga agctgggcat
      541 gtactccctg tataaataca taggggatat gatagctgtc gagcatctca tacagattca
      601 catgccggca gaaaatctgg ctcttgctcg gctcgatgat gagcccgtcg atatccttct
      661 gcagaagctc ctcgagacac tttgcctcaa ggcttcttga gttgcgcgta tttttcagca
      721 gaatactgta accgttctcc gtcagcacct ggtcaattcc ctgcacgatg cgcggaaaaa
      781 tataatccga aagataagtt gtcacgaccg cgatattttt ggacggcgtc gtgtgccgta
      841 acatctccga gcagaaggtt ccgcggccat gttccgcata tatgtatccc tcattctgca
      901 aaatctgcag tgccttgcgc accgtctgcc ggctgacccc gtattccgcc gacagttcat
      961 tctccgaagg aagcttctct cccgggcgta tctcccccga catgatcttg ccatggagct
     1021 gctccatcag atcataatat tttaatctct tatccattcc gtttgatccc cctgaatgcc
     1081 ttctctcttt gcgcgccgtc agcccgttat cccgacagcc tccttcgcca ggcggtctgc
     1141 ctcctcattg cccatgacgc ccgagtgtcc ctttaccttc acaaaagaaa gccggatctt
     1201 atcctccacc aagcgcacat actcgtagta ggccaccgta ccctttttat tgcgcttcca
     1261 gagtcctttt gcccacatct cgattcccat ataatcgtaa tagatcgtaa gatcctttaa
     1321 gccaagttcg agcgcctttt cgatggctgc catgctccct aaaacctcgc ctgccacatt
     1381 gcgcatcgat gccatctccg gctccttccc cgcaccctgc agcacttctt ttccgccggc
     1441 atgaatcaaa aaacctccat aaccgtacac gccggtaccg gcgtgaaaag agccatccac
     1501 gaacgcgtaa ttctccggca gatcctctgt ctctccggca gattccgcac ctctcgtctc
     1561 tgccttgccg tctccggctg ttcctgattt ctgctgtgct gctccttgcg ttgccttctg
     1621 ctgcgccgct ccttttgcat gagcttctcc cccacgcgcc ggtgcgcttt ccagaaacgt
     1681 ctccgcttct tccagcgtct taaaactctt gtaaaccgct ccggggaacc cgtgcaccat
     1741 cgccttacac tcgtcccatt ttgtgtatat tcccggtttc ttaccaaccg ctaccgcata
     1801 atattttact gccatctcgt catcctttat ttcgatattt tataatacaa tataccatat
     1861 aattcccgca cataaaaggt cggaaggtag agaaaaaatg tccggctcgg tgtgctgtag
     1921 cttgtaatgc caagtctctt tgccgtctga ttcgcccggt actcatggaa ttcactggtt
     1981 ataatcgtga cggacccgtc gattccgtgc tgttgtaaca attccatgga atattgcaga
     2041 ttttccgccg tcgtcgtgga ccggtcctcc agaagcagtc tcgccccgtc gatcccccgc
     2101 ccggtcaggt aacggtacat gcactctgcc tcgctgatct cctctccatc tccctgtccg
     2161 ccagataata cgcacacagc ttttggattc cggttcagat actcatacgc cgcgcaaagc
     2221 cgttcgctca acacccggct cggccgctcc cccttgacgc tgcatcctaa gaccaccgcc
     2281 gtcgtattct ccggcgcacg gtttgccgcg cttcttatca tccccgccgt ctccactgtc
     2341 acgataagca gaataaaaag gagcaccatg cccagggcgg acaatcccgc tctgccaccg
     2401 cgcgtctgcc aaagtctccc tgccacatcc tgtacctgcc ggtaaaaaat accgtacagc
     2461 agcacaaggg ctgacaccgc cattcccgta atgttaccga tattaaccac gacaaatgcc
     2521 ggaatgaaaa accacaggaa aaatacggct ccaactgcca ttatccacca atgcatccgt
     2581 gacattcttt ctcctttcca aaaaagcaat gagcggcacg ccgaagcggg ccgctcttgt
     2641 cctctcttgt tacaaatctt gttacgaaat atccagctta cagttcgcac gcatttttaa
     2701 cggacattca taaacgttct cattattctc ttttgcaatg taagaaagcg gacactttct
     2761 gcagtcgcct ttattaaaat ctccgccaac ctcaaatttt aaggttgccg tcttcacacc
     2821 cttcaccatc tcttcctgcc ggatgtgatc ccagctcaat acctgactgc agccggcaca
     2881 cttgctctcg ttcaatgtca ccacgcgctt gcaggtcgga caggcataaa acttcttacc
     2941 ggacggctcc accagttcca caacttccgc cggaaactgc ttaatcaatt cctctctcaa
     3001 attgtcctgc atgatccaat cgctccttta tcctgagttt gcactggtac tatcatacca
     3061 caaaggcggc ggtactgtaa accgctttcc ttcgttttcc gctcagattc cgtttcatta
     3121 ttctccgggt tccgctactt ttccccctgt tgctgccgga tcatgtccat gatgtaatcg
     3181 atcgtttcct gccactgctc cctgtccgta atcccggcct cgccgtctcc cttctgcgat
     3241 ccgtaattgc cgaacccggc atggttgccg ccctctaaga catactcgcg gcagtccgcc
     3301 gcaagaccga ttgcttcctg atatttttcc atattcaaaa cgccgtcctc gctcccgtag
     3361 agcgagagca ccggaagcgt ctgaagtttc ctggtcgggt acgccgccag aaaaataagt
     3421 cccgcgagct ctgactcatg ctttgccgca tagctgcccg ccatggctcc gcccagagaa
     3481 tgtcccgcca gataccagcg ctttacctct ccaaactcct gccgcacctc atccgccgca
     3541 ttcggcttta aaatggcaag gtgcgccggc atctttaccg tcacgcaaaa gattccctgc
     3601 tgcgcaagcc catccataac cggtgcgtac gccgtctctt ccaccttggc tcccggataa
     3661 aagatcagtc ccgtctcact ctgcggatct ccgtaagcga tgtaggcatc cgtctccgat
     3721 acctctgttg tctgaagttc atagtccttc gcatgataat aatctgccgt ataggtatta
     3781 aatcccacaa tcacggctgc cacgattaaa accgtaaaaa taattctgcg caccgctttt
     3841 gcatgcttca ttgtctctca tcctccgaca ccggaatctg cttccgttac tttcggatat
     3901 ctgcaaccac ctcatccgga atgacgaact gtacgcatcc catatacatc ggtgccacat
     3961 caccctcgtt aaagcatatc acaagatggc cgtccgaatc cagataaaat gaagtctcat
     4021 ccgtaatttt gtcaaaattc cattccggaa cctcggcatt atccacccag tacatcttac
     4081 cgtcatcctc cgccatctgc tgctgcatct gctccttgat attctcgctg atcggggtga
     4141 tataatccgc gccactctgg aataaatccg aaagtgtcag acgctccccc gtctttaagt
     4201 caatcgtata ataatagtcc cactccgcgc cgctgcctgc cgcctgatag cacatcagct
     4261 ttaacgtaaa gtaatcgtcg gtcgtgctga taacttcgtg cttgaccacg acctcctgat
     4321 agccctcttc ttccttcctg ctctcctcga actccgatac gatctgatcc gtaatctttt
     4381 caatctccgc attgatctct gccgttgtct tttttaagtt ctcctgcacc tggacctcag
     4441 tgtccgtcat ctgttccgta tcgctctcct ttaccgtcag ctccggcgtc ttaatgtccg
     4501 ccgcatgacc tccgttgtcg tactgataat cccggaacgt cacagcctcc acgagttttc
     4561 cgaccaccgg tagattcccc atggcatacg ccacgctccc cgaggtgttc ggcagcacca
     4621 cgaagactgc cgccactnnn nnnnnnngat gatgccgctc aaaaacggcc agttttacga
     4681 tgttgtgttc cgggtccaca ccgggagcct tgcccggatg ccgctgccac aatatttctc
     4741 acggtatgca tttttctgct ctcgtttcgt ttttccatct ttgcttcctc gatccgtttt
     4801 ttcatctgct ccacctgctc gccggacatt tccgatccgg catactgtcg ctttaattcc
     4861 tcgatctttt tacaatcctg tttcttattt cccatatgcg gcaccatcct ttcctctatt
     4921 cccactgcca tgtatccgta agttccagcc gtagcttctt taatccgcgg tacagccggc
     4981 tctttaccgt attctggttc tcccccagca cgcgcgcaat atcctccagc ttcatgtcct
     5041 caaaatagcg cagttcaatg accgctttat cccgttcttc catggcgtcc aatgcccttc
     5101 ttagatcaat gttctcatag cggtcttctc ttccctcttc cgccggcatc tcatccaggg
     5161 aaacttcccg gttctttttt acatatccga agatctcgtt taacataatg cggtaaatcc
     5221 atgtctgcgc atattcctcc tgctttagcg catcgctgtt tttgatcgcc ttgtacgctc
     5281 cgttctgtac gatatccgcg gcatccgcct catgatgtac gtaactgtag gcaagacgat
     5341 agtatttgtt gtaattttcc agcaaaagct gttctatctt ttgttcctta tggtttttcc
     5401 cggagaaaaa catacgatct cacacctcct tttaactatt agaccgtccg gacaccaaaa
     5461 aagtttcgct gccgtataaa aaattatatt ttctccgatc cggttccgtt ttttccactc
     5521 tcctgtctgc acagttccgt caaaaaccgg tacattccct cctggtaaat cgggcgctcc
     5581 accacacaat ccactcctgc ggcaagcatc tgacactcca ccagttcctt ctcattggtc
     5641 gaacagcaga tcgtaaggct tcccttcgga aaattttccc gcaccgaccg gataaacggc
     5701 tcaccgccct gcatatacca gttcacaaaa cacagatcat aggcgttgcc cttagacgcg
     5761 gctctcctta aatacgccac ggcgtccgcc ccgttttgca ccacgtcaac cggaagcccg
     5821 agacgtgcaa gaaccgctgc atgataatct ctcgacagtt cattgtcgtc cagaagaagc
     5881 gtctttaacc ccgcaaaact tttgggctgt ccgatcgctt ttccgattcc gaaaggcagg
     5941 aacaccctgc agagaatctg cgcatcctcc tgtacttcca cacacatcgt tccgtgcaaa
     6001 agttctacga ggctcttgat cagcggatag tcttctcctc cgtcaatgct gccctgcacg
     6061 gcaagttcca gttcaagctc atcctccccc gttcttactt cccgatagcc tgtaaaaatc
     6121 cggcatcccg gccgcgcgtt gatatacgct ttctttagca gtcttgccca gatctgcttg
     6181 agcctcctga catcgcccac cagatattcg cagaaaatct caccctcctg caccgtcacc
     6241 tcaatcccca tttccgtaca gattgtcctg cactctgagg tcagttccgc aaccacatcc
     6301 gaaatctgaa aggtgtcgca gacactctcg cccgcaccta caatctgccc gtccaaaaca
     6361 ctgtccgtca gttcccggat gtgctccaga tgcctgtaca tatcctccag tcccacagac
     6421 ttattttccg ttctctgacc tgtcatttct ctgtaacact cctcgatcac cgcgacgtcc
     6481 gtgcgcaccg tccgcgccac ctgaaacagc agatgttctc tgaccgtgct taaatgccgc
     6541 tggctctcaa gccgctccgc cagtgtctca tagcgtgtca tgatattgga cacccgcttt
     6601 tttaccacct ccggctcgca gggagaaaat ataaaatggt actaccttgg aagcagcggc
     6661 gctgtgaaga aaggctggat caatg
//
LOCUS       DBMDQJ010000029         6684 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4725_length_6684_cov_1.523470, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000029 DBMDQJ010000000
VERSION     DBMDQJ010000029.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4725_length_6684_cov_1.523470"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /gene="rsmB"
                     /locus_tag="ACZO9R_01100"
     CDS             complement(<1..1321)
                     /gene="rsmB"
                     /locus_tag="ACZO9R_01100"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006780422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HIR0911702.1"
                     /translation="MTTDSVNTRELILEILLEVTEKGQYSHLVIRSVLDKYQYLDKRE
                     RAFLTRVAEGTIQRQIELDYILDQFSKVKVRKMKPVIRAILRMGVYQLKYMDSVPASA
                     ACNEAVKLAKKKGFGQLSGFVNGVLRGVSRSLDQLTYPEESADAVKAWSVCYSMPEWI
                     VAQWIGDYGKERTKEILAASLLEAPITVRTNLERITPEVLAERFKEEGVTAVPLDTEK
                     YPGLSYAFTIFGFDHLNGLSSFQEGLFYVQDISSMMVAEYAAPKEGDHCIDVCAAPGG
                     KSIHLAEKLRGTGHVEARDVSEQKVALIDENIKRCRLTNITAKCQDATVLDEASVRTA
                     DVLIADLPCSGLGVLRRKTDIKYRMNPEGEESLVALQRQMLSVVWQYVKPGGVLVYST
                     CTVNRLENDENRAWFLNEYPFEPVDISGRLGIDFQEDSLKEGYIQLYP"
     gene            complement(1311..2027)
                     /locus_tag="ACZO9R_01105"
     CDS             complement(1311..2027)
                     /locus_tag="ACZO9R_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HIR0911703.1"
                     /translation="MPYSYYYWGFDPTYILVLIGAVICLVASARVKSTYNKYASYRSM
                     SGMTGAQAAERILHSAGIYDVSVQHVSGNLTDHYNPSNKTLNLSDSVYGSTSVAAVGV
                     AAHECGHAIQHQQGYVPLALRSAIVPVANIGSTLAWPLILIGLFFTSNTGAFLIDLGI
                     LCFSFAVLFQLVTLPVEFNASHRALRILGEQGILSDSELPYTRKVLKAAALTYVASAA
                     ASILQLLRLVLLFGGRRRDD"
     gene            complement(2033..2968)
                     /gene="fmt"
                     /locus_tag="ACZO9R_01110"
     CDS             complement(2033..2968)
                     /gene="fmt"
                     /locus_tag="ACZO9R_01110"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR0911704.1"
                     /translation="MRVIFMGTPDFAVGTLEEIIKAGHEVVLVVSQPDKAVGRSKALR
                     YTPVKACALEHGIEVYQPQKVRDSECIERLRTYHPDIIIVEAFGQIIPRAILDMPRYG
                     CVNVHASLLPKYRGAAPIQWAVINGDTVTGVTTMRMDEGLDTGDMILKEEVGIREDET
                     GGSLFDRLAEVGAKLCVRTMEAIEAGTATYTPQDNSQATHTAKIYKELGSIDWKQGAK
                     EIECLIRGLDPWPSAYTRLGDKMLKIWKAKVVPEESGAEPGCIVKVEKDHILVQTGKG
                     MLAISELQLEGKKRMPVEAFLNGYEIEEGTYFKKG"
     gene            complement(3002..3481)
                     /gene="def"
                     /locus_tag="ACZO9R_01115"
     CDS             complement(3002..3481)
                     /gene="def"
                     /locus_tag="ACZO9R_01115"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005950048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR0911705.1"
                     /translation="MALRTIRIQGDPVLTKKCREITEITPKIRELAEDMLDTMYEANG
                     VGLAGPQVGVLKRIVVIDIGEGPVVMINPVILETSGEQTGDEGCLSLPGKAGTVTRPN
                     YVKARAFDLDMEEYEIEGTELMARAICHELDHLDGHMYTEKVEGPLHEVTYESDEEE"
     gene            complement(3498..5735)
                     /gene="priA"
                     /locus_tag="ACZO9R_01120"
     CDS             complement(3498..5735)
                     /gene="priA"
                     /locus_tag="ACZO9R_01120"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR0911706.1"
                     /translation="MAEYAKVIVDISQEKLDKTFEYRIPPYLTQRIAVGMQVYIPFGS
                     RRITGYVVELSDEAEYDEDKLKNIAGIVTDSVPIESHLIALAGWMREHYGGTMNQALK
                     MVLPIKEKKKAVEHKRVRLALHPVEAKNQLGAYERRHSTARAKLLRALLEEEELDWDL
                     VTHKLGVSGSVIRAMEESGVVKVIRETQYRNPVSHLTSRGYGLTLNDEQQEAVDAVWS
                     DYEKGIRSTYLIKGVTGSGKTEVYMELIAKMQKAGRQAIVLIPEIALTYQTVLRFYNR
                     FGDRVSILNSRMSPGERYDQFLRTKNGEIDVMIGPRSALFTPFANLGLIIIDEEHETS
                     YKSETVPRYHARETAIARAAMLDASVVLGSATPSVDSYYRAKRGEYRLLTLTKRVKER
                     PLPACGVVDLRQELREGNRSILSRRLDGLMEQRLRAGEQTMLFINRRGVAGFVSCRAC
                     GHVIKCPHCDVSLSQHANGRMVCHYCGYTEPVPVQCPACGSKYISGFKAGTEKIEMVV
                     RKRFPEARVLRMDMDTTRGKEDYEQILSAFANHEADILIGTQMIVKGHDFPDVTLVGV
                     LAADLSLHVGDYRAAERTFQLLTQAAGRAGRGNKPGEVVIQTYDPEHYSIETAKAQDY
                     DAFYEQEIAYRKIMLYPPVWNMLVILCASKSEEAADAMAAELAAAADAYTGKNTKEKV
                     YLVGPVDAAIAKVNDIYKKIIYLKAENYDTLVKIKNRTEVYARESRYYKNVTVMFDFN
                     PVNGF"
     gene            complement(5714..>6684)
                     /locus_tag="ACZO9R_01125"
     CDS             complement(5714..>6684)
                     /locus_tag="ACZO9R_01125"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008765 -
                     UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR0911707.1"
                     /translation="KMQRVEGIFFDIGMFLNIEPDHIGEGEHASFAEYLFCKSRLMRQ
                     CRIGIVNRDDPHTERILQGHTCSVETFSLRHPADVTAENIRFFMQSGELWGSFLVRGK
                     TAVYPVRMQLPGTFNVYNALAAIKAAGHLGAAESDICLALRSAKVPGRCENVRQSEDY
                     AILIDYAHNEMSLKNLLEMLRGFKPRRLIVLFGCGGNRSKLRRSKMGETAGHLADFTI
                     LTSDNPRWEDPEEILDDIEDGIRGTGGQYVRIADRREAVRYAMAHAMENDMLVLAGKG
                     HEAYQEICGVRYPLSEYELVEEARRSPGMGENTDRSEVDRWRNMRR"
BASE COUNT         1400 a         2167 c         1479 g         1638 t
ORIGIN      
        1 ccggatagag ctggatgtat ccctctttca ggctgtcttc ctgaaaatca atccccagac
       61 gaccactgat atcaaccggc tcaaatggat attcgtttaa aaaccatgcc cggttttcat
      121 cattttccag acggttgacc gtgcaggtgc tgtaaaccag cactccaccc ggcttcacgt
      181 actgccagac caccgacagc atctgcctct gcaatgctac aagcgattcc tccccttccg
      241 ggttcatgcg gtacttgata tcggtctttc tgcgcagcac cccgagtccc gagcagggaa
      301 gatccgcaat cagcacatcc gccgtgcgaa ccgatgcctc gtccaaaaca gtcgcatcct
      361 gacacttcgc cgtgatattc gtaagccggc atctttttat attctcatcg atcagcgcca
      421 ctttctgttc gctgacgtcc cgcgcctcca catgaccggt acctcttaac ttttccgcaa
      481 gatgaatgct cttgccgccc ggcgccgcac acacgtcaat gcagtggtcg ccctccttgg
      541 gcgccgcata ttctgccacc atcatggagc tgatgtcctg cacatagaac agtccttcct
      601 gaaaactgga aagaccgttt aaatgatcaa acccaaagat cgtaaatgca tacgaaagtc
      661 cgggatattt ttccgtatcc agaggaaccg cagtaacgcc ctcctcctta aaacgctctg
      721 cgagcacctc cggcgtgatc cgttccagat tcgtgcggac ggtgatcggc gcttccaaaa
      781 gggacgctgc aagaatctct tttgtgcgtt cctttccgta gtcaccaatc cactgcgcaa
      841 cgatccattc cggcatcgag tagcacaccg accaggcttt tacggcatcg gcgctctcct
      901 ctggatatgt aagctgatcc aggctgcgcg aaacaccgcg cagtaccccg ttcacgaaac
      961 cggacagctg tccgaagccc tttttcttcg caagctttac cgcctcgttg caggcggcgg
     1021 atgccggtac actgtccata tatttcagct ggtatacgcc catcctgaga atggcgcgga
     1081 tcaccggctt catttttctg acctttactt ttgagaactg atccagaata tagtccagct
     1141 caatctggcg ctgaatggtg ccctccgcca ctctcgttaa aaatgcgcgc tctcttttgt
     1201 ccagatactg gtacttatcc agcaccgagc ggatcaccag atggctgtac tgtcccttct
     1261 ccgtcacttc taaaaggatc tcaagaatca gttcacgcgt attcacggaa tcagtcgtca
     1321 cgtcttcttc ctccaaataa taacaccaga cgaagcagct gcagaatcga tgccgccgca
     1381 ctcgccacgt aagtaagcgc cgccgccttc aacacttttc gcgtataagg cagctcactg
     1441 tcgcttaaga ttccctgctc tcctaaaatg cgcagcgcgc ggtgtgatgc gttgaactcc
     1501 accggaagcg tcacaagctg aaacagaaca gcgaacgaaa aacaaagaat tccaaggtca
     1561 atcaaaaatg cccccgtatt gctggtaaaa aataatccga ttaaaattaa cggccatgcc
     1621 agcgtagaac cgatatttgc caccggcacg atcgccgagc gcagcgcaag cggcacatat
     1681 ccctgctgat gctgaatggc atgtccgcac tcatgcgccg ccacaccgac agccgccacc
     1741 gatgtacttc catacacgga atccgacaga ttcagcgtct tgttcgacgg attgtagtga
     1801 tcggtcagat tgcccgatac atgctgcacc gacacatcgt agattcctgc cgagtgcaga
     1861 atccgttccg ccgcctgtgc tccggtcatg ccggacatgc tgcggtaaga tgcatacttg
     1921 ttgtatgtcg acttcacccg tgcagatgcg accagacaga tgactgctcc gatgagcacg
     1981 agtatatagg tcggatcaaa tccccagtaa tagtatgaat atggcatagt aatcaccctt
     2041 tcttaaaata tgtgccctct tcaatctcat atccgttcag aaatgcctcc accggcatgc
     2101 gcttcttgcc ctcaagctga agctcactga tggcaagcat tccctttccg gtctgcacca
     2161 gaatatgatc tttttccact ttcacgatgc agcccggctc cgccccggac tcctcaggaa
     2221 cgaccttcgc cttccagatc tttaacatct tatcgcccag tctggtgtag gcgctcggcc
     2281 acggatccag cccgcggatt aagcactcaa tctctttggc gccctgtttc cagtcaatgc
     2341 ttcccagttc cttataaatc ttggcggtat gtgttgcctg tgagttatcc tgcggcgtgt
     2401 aggttgccgt tcccgcctcg atcgcctcca tcgtgcgcac gcacagcttc gcgccgacct
     2461 cggccagccg gtcgaacagg ctgccgcccg tctcatcctc gcggatcccg acttcttcct
     2521 ttaagatcat atctccggta tcaagtccct catccatacg catcgtcgtc actccggtga
     2581 cggtgtcgcc gttgatgacc gcccactgaa tcggcgcggc accacgatac ttcggaagca
     2641 gcgatgcgtg cacattcaca cacccgtagc gcggcatatc cagaatcgct ctcggtataa
     2701 tctgcccaaa agcctccaca attatgatat ccggatgata tgtccgcaga cgctcgatac
     2761 actcgctgtc tctgactttc tgcggctgat atacttcaat gccatgctca agcgcgcatg
     2821 ccttgaccgg tgtatagcgg agcgccttgc ttcttcccac cgccttatcc ggctgggaga
     2881 caaccagtac cacctcatgt cccgctttta tgatttcttc caacgttccc accgcgaaat
     2941 ccggtgttcc catgaatatg actctcatca ctgacctcca ttttcacaga tcatccctgc
     3001 tttattcttc ctcatcactc tcataggtga cttcgtgaag cggaccttcc accttctcag
     3061 tgtacatgtg tccgtccaga tgatccagct catgacagat ggcacgcgcc atcagctctg
     3121 tcccctcaat ctcatactct tccatatcca gatcaaacgc ccgcgctttc acgtagttcg
     3181 gcctggtcac ggtgcctgcc ttgcccggaa ggctcagaca gccctcgtcg ccggtctgct
     3241 cgccggaagt ctccaagatc accgggttga tcatcacaac cggtccctcg ccgatgtcga
     3301 tgacgacgat gcgctttaac acgccgacct gcggacctgc aagtcccacg ccgttcgcct
     3361 cgtacatcgt atcaagcata tcctctgcca gttctctgat cttcggtgta atctctgtaa
     3421 tctctctgca ttttttggtc aatacaggat ctccctgtat tctgattgtc ctaagtgcca
     3481 tctttcctcc attctgccta aaagccgttc accggattga aatcaaacat cacagtcaca
     3541 ttcttgtagt accggctctc tctggcatac acttctgtcc tattttttat ctttaccagc
     3601 gtgtcataat tttctgcctt gagataaatt atttttttat aaatatcatt cacttttgcg
     3661 atcgccgcgt cgacgggtcc cacgagatag accttctctt tcgtattctt ccctgtatac
     3721 gcatccgctg ccgccgcaag ctctgccgcc atggcatccg ccgcctcctc gctcttagag
     3781 gcgcacaaga tcacaagcat gttccagacc ggcgggtaaa gcatgatctt gcggtacgca
     3841 atctcctgct cgtaaaacgc atcgtagtcc tgcgccttcg ccgtctctat actgtaatgc
     3901 tccgggtcat acgtctggat gacgacctcg cccggcttat tcccgcggcc ggctcttcct
     3961 gccgcctgtg tcagaagctg gaatgtccgc tccgccgcgc ggtagtcccc gacatgcagc
     4021 gagagatctg ctgcaagaac gccaaccagc gtcacatccg ggaaatcatg cccctttacg
     4081 atcatctgcg taccgatcag gatatccgcc tcgtgattgg caaatgccga taaaatctgc
     4141 tcgtagtctt ccttgccccg cgtggtatcc atatccatgc gcagcacgcg cgcttccgga
     4201 aatcgttttc tgacaaccat ctcgatcttc tcagttccag ccttaaaccc gctgatgtac
     4261 ttcgatccgc acgccggaca ttgtaccggc accggctccg tatacccgca gtaatggcag
     4321 accatccgcc cgttcgcatg ctggctcagc gatacatcgc aatgcggaca ttttatcaca
     4381 tgtccgcagg ctctgcaaga gacaaagccc gccacacccc tgcggttgat aaagagcatg
     4441 gtctgttctc ccgcccgcag gcgctgctcc atcagcccgt ctaacctgcg gcttaagatg
     4501 gagcggttgc cctctcttaa ttcctgccgc agatccacca ccccacatgc cggaagcggt
     4561 ctttccttca cgcgctttgt gagcgtaagc agccggtatt ccccgcgctt tgcacggtag
     4621 taactgtcca ccgacggcgt tgccgatccc agcacgacgc tcgcgtcaag catggcagcc
     4681 cgcgcgatgg cggtctccct ggcatgatag cgtggaaccg tctcactctt ataggaagtc
     4741 tcatgctcct cgtcaatgat gataagcccc agattggcaa acggtgtaaa cagtgctgac
     4801 ctcggaccga tcatcacgtc gatctccccg ttttttgtcc taaggaactg atcgtagcgc
     4861 tcccccggtg acattctgga attcagtatg gatacccggt cgccaaaccg gttatagaac
     4921 cgcagcaccg tctgatacgt cagcgcaatc tccggaatca ggacgatggc ctgccgcccc
     4981 gccttctgca tcttcgcgat cagttccata tagacttcgg tcttgccgct gcccgtcaca
     5041 ccctttatca gataggtcga ccggattccc ttctcgtaat ccgaccagac cgcatccacc
     5101 gcttcctgct gctcatcgtt taaagtgagc ccatatcctc tgctggtcag gtgggacacc
     5161 ggattgcggt actgcgtttc ccgaattact ttgacgacgc cggattcctc catcgcccgg
     5221 atcacacttc ccgacacccc aagcttgtgt gtgacaagat cccagtccag ctcctcctct
     5281 tccagcagcg cacgaagcag cttggcgcgg gcggtgctgt ggcgtctctc atacgctccc
     5341 agctggttct tcgcctccac cggatgaagc gcaagccgca cccttttatg ttccacggct
     5401 tttttcttct ccttgatcgg aagtaccatc tttagcgcct ggttcatcgt gccgccgtaa
     5461 tgttcccgca tccagcccgc aagcgcgatc agatgggact caatcggcac actgtccgtc
     5521 acgatccccg caatattttt cagcttatcc tcgtcatact ccgcctcatc ggaaagttcg
     5581 accacatagc cggtgatcct gcggctgccg aacggaatat agacctgcat tccgactgcg
     5641 atccgctgtg tgagatacgg cggaatgcgg tactcaaatg tcttatccag tttttcctgt
     5701 gaaatatcca caatcacctt cgcatattcc gccatctgtc cacctcgctt ctatctgtat
     5761 tctcccccat gccagggctt cgtctcgcct cctccaccag ctcgtattca ctgagcggat
     5821 agcgtacccc gcaaatctcc tgatatgcct cgtgcccttt tcccgcaagc acaagcatgt
     5881 cattctccat ggcatgcgcc atggcatacc gcacagcttc ccggcggtcc gcaatgcgca
     5941 catactgacc gccggttccc cgtatcccgt cctcaatgtc gtccagaatc tcctccgggt
     6001 cttcccagcg cgggttgtcg gaagtcagta tcgtaaaatc cgccagatgt cccgccgtct
     6061 ctcccatctt cgaccgcctg agcttcgacc ggttgccgcc gcacccgaac agtacgataa
     6121 gacgccgcgg cttaaaaccg cgcagcattt ccagaagatt ttttaagctc atctcattgt
     6181 gtgcatagtc aatcagaatg gcataatctt cggactgcct cacgttctcg catcttccgg
     6241 ggacttttgc gctgcggagt gcaaggcaga tatcgctctc cgcggctccc aggtgtcctg
     6301 ccgcctttat ggccgccagt gcattgtaga cgttaaacgt tccggggagc tgcattctca
     6361 ccggatacac cgccgtcttc ccgcgcacca gaaagcttcc ccaaagttct cccgactgca
     6421 taaaaaagcg gatattctcc gccgtcacat ccgccggatg ccgcagggaa aacgtctcca
     6481 cgctgcaggt atgtccctgt agtatgcgct ccgtgtgcgg atcgtcccga ttgacaatcc
     6541 cgatccggca ctggcgcatc aggcggctct tgcagaacag atattccgca aacgaagcat
     6601 gctcgccctc cccgatgtga tccggctcga tgtttaagaa catgccgatg tcaaaaaata
     6661 tcccctccac ccgctgcatc ttca
//
LOCUS       DBMDQJ010000030         6591 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4795_length_6591_cov_1.569800, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000030 DBMDQJ010000000
VERSION     DBMDQJ010000030.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6591)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6591)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6591
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4795_length_6591_cov_1.569800"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1333)
                     /locus_tag="ACZO9R_01130"
     CDS             complement(<1..1333)
                     /locus_tag="ACZO9R_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0911708.1"
                     /translation="MTKDMTSGSPMKLILGFSVPLLFGFLFQQFYNLVDTLIVGRFLG
                     VDALAAVGSTGSLNFLIIGFCMGVCNGFAIPLAHRFGAGDYKAMRAFMMNAVYLAVIF
                     AAVMTVFTVAFCRPILQLMQTPENIIDDAYTYIVIIFAGIPATYLYNLVSGVIRSMGD
                     SKTPLIFLALAAFVNIGLDLLLIIVFPMGVAGAALATVISQAFSGIACVIYSKRKFEI
                     LHTDEEERKVNKGYMKTLCGMGVPMGLQYSITAIGSVILQSAVNTLGSNAVASMTAGS
                     KIGMFFCCPYDALGSTMATYGGQNVGAKKLDRLSGGLKAAGILGIGYSILAFVVLWLC
                     GNQLAMFFVESAQAEVIANVRLFLVCNSAFYIPLAFVNIVRFLIQGMGYSKFAILAGV
                     FEMAARTLVGFVFVPMFGYPAACFASPVAWIFADCFLFPAYFHVLKKTKKML"
     gene            complement(1361..2353)
                     /locus_tag="ACZO9R_01135"
     CDS             complement(1361..2353)
                     /locus_tag="ACZO9R_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR0911709.1"
                     /translation="MRKRWAMGMTACACALSIALTGCQIGNKDIVVSGTLSSRQVFTL
                     DKSACSLKEAKVYLANYQNIYGTAYTIDLWQHDFGDASLLDYVKDITLEELTRVYTMD
                     LLAQSQEVTLSEDETAKVAEAAKEYYASLSEDETAYMDVAETDIVEYYTHYALAQKLY
                     HSLTNGVNEEVSDDEARVMEIMQIYVTDEDRAHEVEQKLAQGDDFASVANNYNELSAI
                     QVTVSRDDLPDEVEEVAFQLDDNAVSGMIAAGNGYYFIKCLNKYNEELTEANKSNIVE
                     KREKEAFDDVYNEFVASLSSRLNTDLWDGIELTTDGSIQTNSFFAVFEKYCGEI"
     gene            complement(2379..3776)
                     /locus_tag="ACZO9R_01140"
     CDS             complement(2379..3776)
                     /locus_tag="ACZO9R_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR0911710.1"
                     /translation="MKNYDVIIIGAGPSGIFCAYELIHAKKDLKVLMIEKGRRIENRE
                     CPKRKTKVCVGCKPCSITTGFAGAGAFSDGKLSLSPDVGGNLPEILGYDKTVELLKES
                     DDIYLKFGADSKVYGVDKEKEIREIRRKAIMANLKLIECPIRHLGTEEGYKIYSRLQE
                     HLLEKGVEMEFGTMVKDIIIEDNQVKGIITDKDETYTAAEVISAVGREGADWFSHICN
                     QHDIETKVGTVDIGVRVEVRDEVMEFLNENLYEAKLVYHTPTFDDKVRTFCTNPSGEV
                     ATEYYDNGLAVVNGHAYKSKDLKTNNTNFAILVSKNFTKPFKTPIEYGKQIAQLSNML
                     CDGKILVQTFGDFKRGRRTTEERLCRNNLIPTLKDAVPGDLSLVFPHRIMVDIEEMLM
                     ALDKVTPGIASDETLLYGVEVKFYSNKVVVNSDFETSVKGLRAIGDGASVTRGLQQAS
                     ANGISVARSILKNIM"
     gene            complement(3850..4107)
                     /locus_tag="ACZO9R_01145"
     CDS             complement(3850..4107)
                     /locus_tag="ACZO9R_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HIR0911711.1"
                     /translation="MYDEMCLDTFLAQQEKLFREPVAESREEAEEFLSDCFATVCENL
                     KEVRAYFKEEGADIAGMSSEDLAEQAEVFALPDGRYLIVEA"
     gene            complement(4134..4760)
                     /locus_tag="ACZO9R_01150"
     CDS             complement(4134..4760)
                     /locus_tag="ACZO9R_01150"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR0911712.1"
                     /translation="MGQIILASASPRRKELLEQIGISFEICPAKGEERITKSVPHEVV
                     EELASQKAIEVASMVRTYEETHQELVTPSDLMVIGADTIVSCGGQILGKPKDERDACR
                     MLKLLSGGTHQVYTGVSIVLLDASGKAGQVTFHEKTDVTMRAMSEEEIRRYVATGEPM
                     DKAGAYGIQGKCAIFVDKIDGDYNNVVGLPVAAIYRELKKLGIDIYLW"
     gene            complement(4825..>5423)
                     /locus_tag="ACZO9R_01155"
     CDS             complement(4825..>5423)
                     /locus_tag="ACZO9R_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR0911713.1"
                     /translation="VPPFVQKKMEQEETGGQNQGALRGTAVHRVMECYQFSAAGVTEQ
                     IEAMLAGGQITEEMKRLVKPALIETFLTSAAGERMKRAETAGTLYREKPFVMGFTGEE
                     LAAFGFGDGEKCADDKELTLIQGIIDVFWIEEDGIVVLDYKTDRVKTGKELADRYASQ
                     LKLYGEALERIYNHGSERTLRVKERLLYSFRLGAVIPV"
     assembly_gap    5424..5433
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5434..>6591)
                     /locus_tag="ACZO9R_01160"
     CDS             complement(<5434..>6591)
                     /locus_tag="ACZO9R_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR0911714.1"
                     /translation="PIAGLTDEELGRLRAHDRSVPFCECVLARCRELAQSEEPLAEGY
                     EKKLWEFWKLYERLRALVPDTPIHELIEIVLKESGYGNYAAAMPAGARRRANLRMLVE
                     KAIAYENTSYKGLFHFVRYIDELQKYDVDFGEADMVGENEDVVRIMSIHKSKGLEFPV
                     VFVCGLGRNFNKQDTRSRMVLHPELGIGIDWIDGERRIKTPTILKRAIAKQIELENLG
                     EELRVLYVAFTRAKEKLILTGCRKDAENDLAALREAAQEELFPETEHVPLPYLLRESA
                     AGYFDWLLPAVFSYHGRYQVRVVPAEELLDAESRHMKEETENLEQCLSRIAAADAADV
                     AEFDGKFSFTYPYERDLERKNKYSVSELKHRAMREKLLEEETDVVPAFLET"
BASE COUNT         1487 a         2051 c         1368 g         1675 t
ORIGIN      
        1 tcaacatttt cttcgtcttc ttcaacacat ggaaatatgc cgggaacagg aaacagtcag
       61 caaaaatcca tgcgaccggg cttgcaaagc acgctgccgg atagccgaac atcggaacaa
      121 atacaaatcc gaccagggta cgcgccgcca tctcaaagac gcctgccaga attgcaaact
      181 tgctgtatcc cataccctga atcaaaaagc gtacaatatt cacgaaagcc aacggaatgt
      241 agaaggcact gttgcatacc agaaacagcc ttacatttgc aatgacctcc gcctgtgcac
      301 tctccacgaa aaacatggca agctgattgc cgcaaagcca cagcaccaca aacgccagga
      361 tcgaatatcc gattcccaga attcccgccg cttttaatcc gccgctcaag cggtctaact
      421 tcttcgctcc cacgttctgt ccgccgtagg ttgccattgt agaaccgagc gcatcatacg
      481 ggcagcagaa gaacataccg atcttgcttc ccgccgtcat ggatgccacg gcattcgatc
      541 cgagcgtgtt gactgcgctc tgtaggatta cgctgccgat cgcagtgatc gaatactgta
      601 atcccatcgg cacacccatg ccgcagaggg ttttcatgta gcccttattc actttccgct
      661 cttcctcatc ggtgtgcagg atctcaaact ttctcttgct gtagatgacg cacgcgatac
      721 cggaaaatgc ctgcgagatc acagttgcaa gcgctgctcc ggcaactccc atcggaaata
      781 caatgattaa aagcagatcc agtccgatat tcacaaatgc agccagcgcc aggaaaatca
      841 gaggcgtctt gctgtcgccc atcgacctta tcacgccgga caccagattg tacagatagg
      901 ttgccggaat tcccgcaaaa ataatgacaa tgtaggtgta ggcatcatca ataatattct
      961 ccggcgtctg catcagctgt aagatcggcc ggcagaatgc caccgtaaac accgtcatga
     1021 ccgccgcaaa gatcaccgcc agatagacag cattcatcat aaacgcacgc attgccttat
     1081 aatcaccggc tccaaaccgg tgcgccagag gaatggcaaa accgttgcac actcccatgc
     1141 agaatccaat aatcagaaag ttcagcgaac cggtcgatcc cactgccgca agtgcatcca
     1201 cgcccagaaa tcttccgacg atcagagtat ccacaagatt ataaaactgc tgaaacaaaa
     1261 atccaaacaa cagaggcacc gaaaatccta aaatgagttt catcggtgaa ccgcttgtca
     1321 tatctttcgt catagaatct acctcatttc atactgtatc tcagatctca ccacaatact
     1381 tctcaaatac tgcaaaaaag ctgtttgtct gaatactgcc atccgtggta agctcaattc
     1441 catcccacag atccgtgttc agcctcgagg acagggatgc aacaaattca ttgtagacat
     1501 cgtcaaatgc ttctttttcg cgcttttcca caatgttgga cttattcgct tccgtcagct
     1561 cttcattata tttattcagg cacttgataa aatagtatcc atttccggca gcgatcattc
     1621 cggagacggc attgtcgtcc agctgaaatg ccacctcctc cacctcgtcg ggcagatcat
     1681 ccctcgatac cgtcacctga atcgccgaca attcgttata attgttggcg acggaggcaa
     1741 aatcatcccc ctgcgccagc ttctgctcca cctcgtgcgc ccggtcttcg tccgtgacat
     1801 aaatctgcat gatttccatc acgcgcgcct catcgtcgct gacctcctcg ttgacaccgt
     1861 tcgtcagtga atgatacagc ttctgcgcca gcgcatagtg cgtataatac tccacaatat
     1921 ccgtctcggc gacatccata taggcagtct catcctccga gagggatgca taatattcct
     1981 tcgctgcttc cgccaccttc gcagtctcat cctccgagag ggtcacctcc tgcgactgtg
     2041 ccagcagatc catcgtgtat acacgggtga gctcctccag cgtgatgtcc ttcacatagt
     2101 caagcaggga tgcgtctccg aaatcatgct gccagaggtc aatcgtatat gctgttccat
     2161 agatattctg ataatttgcc agatatacct ttgcctcctt taagctgcat gcagacttgt
     2221 ccagtgtaaa cacctgcctg ctgcttaacg ttcccgagac cacaatatct ttattgccaa
     2281 tctggcatcc cgtaagagct attgacagcg cacatgcgca cgctgtcatt cccatcgccc
     2341 atctttttct catcgtgaaa caccctttat ctcaacatct acatgatatt tttcaagata
     2401 cttctcgcca cagaaattcc gttcgcggat gcctgctgca gtcctctggt cacggacgcg
     2461 ccgtccccga tcgcacggag tcccttgaca ctcgtctcaa agtcagagtt caccactacc
     2521 ttattggagt agaacttgac ctccacaccg tagagcagcg tctcatcact tgcaataccc
     2581 ggcgttacct tatcgagagc catcagcatc tcctcgatat ccaccatgat ccggtgcggg
     2641 aataccaggg aaagatctcc cggtaccgca tcctttaagg tcgggatgag gttgttgcgg
     2701 cacaggcgct cctccgtcgt tcttctgccg cgtttaaagt ctccgaatgt ctgcaccagt
     2761 atcttgccgt cacagagcat attagagagc tgtgcgatct gcttgccgta ttcgatcggc
     2821 gtcttaaaag gcttcgtgaa attcttcgat accagaatgg caaagttggt attgttggtc
     2881 tttaaatcct tggacttgta ggcgtgtccg ttgaccaccg ccagtccgtt gtcgtagtat
     2941 tcggttgcaa cctcaccgga cggattagtg cagaaggtac gcaccttatc gtcaaacgtc
     3001 ggtgtatggt acactaactt cgcctcgtag aggttctcat ttaaaaattc catcacctca
     3061 tcacgcacct cgacacgcac accgatatcc acggtgccga ccttcgtctc aatatcatgc
     3121 tgattgcaga tatgggagaa ccagtctgca ccctcacgtc ccacggcgga gatgacctct
     3181 gctgccgtgt atgtctcatc cttgtcggta atgataccct tgacctggtt gtcctcgatg
     3241 atgatgtcct tgaccatggt gccaaactcc atctcgaccc ctttctctaa caggtgctcc
     3301 tgcagtctgg aatagatctt gtagccttcc tccgtgccga gatgacggat cgggcattcg
     3361 atcagcttta agttcgccat gatcgctttt ctacggatct cgcggatttc cttctcttta
     3421 tccacaccgt acaccttgga atccgcaccg aatttcagat aaatatcatc cgattccttt
     3481 aacagctcca cggtcttatc gtagcctaaa atctccggca ggttgccgcc gacatccggc
     3541 gataaggaca gtttgccatc ggagaaagct cccgcaccgg cgaatccggt cgtgatggaa
     3601 cacggcttgc atcccacgca taccttggtc ttacgcttcg gacattcacg gttctcaatc
     3661 cgtcttcctt tttcaatcat taacactttc agatcctttt tcgcatgaat cagctcatac
     3721 gcacaaaaaa ttccggaagg accggctcct ataatgatca catcataatt cttcattaca
     3781 ttccctcgtt ctttctatat attctaccat tctgttatgt atcttcctac gttctccaaa
     3841 gcagacggct taagcttcca cgatcaggta tcttccgtcc ggcagtgcga acacttctgc
     3901 ctgctccgcc agatcctcgc tgctcattcc ggcgatatcc gcaccctctt ccttaaagta
     3961 cgcccgcact tccttcagat tctcacagac cgttgcgaaa cagtccgaaa gaaattcctc
     4021 cgcctcttcc ctgctttctg ccacaggttc ccgaaacagc ttctcctgct gcgccaaaaa
     4081 tgtatccagg cacatttcat cgtacatatc ttttcctccg ccaacgtttt attttaccat
     4141 aaatagatat caattccaag cttctttaat tcccggtaaa tggctgctac cggaagccct
     4201 acaacattat tatagtcgcc atcgattttg tcaacaaaaa tcgcacattt cccctgaatt
     4261 ccgtatgcgc ccgccttgtc catcggctcg ccggtggcga catagcgccg gatctcctcc
     4321 tcgctcatcg cgcgcatcgt gacatcggtt ttctcatgaa acgtcacctg accggccttt
     4381 ccggacgcat ccaaaagcac gatcgacact ccggtgtata cctggtgtgt gccgccggag
     4441 agaagcttaa gcatgcggca ggcatcacgc tcgtctttgg gcttccctaa gatctgtccc
     4501 ccgcaggaga caatcgtgtc ggcgccgatc accataagat ccgagggtgt caccagttcc
     4561 tgatgcgtct cctcgtaggt tcttaccatg gatgccacct cgatggcctt ctgcgacgcc
     4621 agttcctcca cgacttcgtg cggtacgctc ttcgtgatcc gctcctcacc tttggccggg
     4681 cagatttcaa acgaaatccc gatctgctct aacaattcct ttctccgcgg tgaggcggat
     4741 gccagtatga tctgtcccat ctctttttct tcctttctga tgttcttatc tctgaaatct
     4801 atcgtttctt atgttccatt tacgttacac cggtatcact gcgcccagcc ggaacgagta
     4861 gagaagccgc tccttcacgc gaagcgtcct ctcacttccg tggttataga tccgctccag
     4921 cgcctcgccg tagagcttta actgcgaagc ataacggtcc gccagttcct tgccggtttt
     4981 cacacggtcc gtcttatagt ccagcaccac aatgccgtcc tcctcaatcc agaacacatc
     5041 gatgattccc tggatcagcg tcagctcttt gtcatccgca catttctctc cgtctccgaa
     5101 tccgaatgcc gcgagctcct cgcccgtaaa tcccatcaca aacggtttct cccggtacag
     5161 cgttccggca gtctccgcac gcttcatccg ctctcccgcc gccgatgtga gaaacgtctc
     5221 gatgagagcc ggttttacca ggcgcttcat ctcctccgtg atctgtcctc cggcaagcat
     5281 cgcctcaatc tgttccgtga ctcctgccgc cgaaaactgg tagcactcca tcaccctgtg
     5341 aaccgcagtt ccgcgcagtg caccctggtt ctgtccgccg gtttcttcct gctccatttt
     5401 tttttggaca aacggcggaa catnnnnnnn nnnccgtctc taaaaacgcc gggacaacat
     5461 ccgtctcctc ctccagaagc ttttcccgca tcgcccgatg ctttaattcc gacacggaat
     5521 atttgttttt gcgctccaga tcgcgctcgt acggataggt gaatgaaaac ttcccgtcaa
     5581 attccgccac gtctgccgca tccgccgctg caatgcgtga cagacactgt tccagattct
     5641 ccgtctcctc cttcatgtga cggctctctg cgtcgagcag ttcctccgcc ggcacaacgc
     5701 gcacctgata gcgtccgtga taggaaaaca ccgccggaag cagccagtcg aaatagcctg
     5761 ccgcgctctc ccggagcaga tacggcagag gaacatgctc cgtttccggg aaaagttcct
     5821 cctgtgccgc ttcccttagc gccgccagat cattttctgc atcttttctg caccccgtta
     5881 agatcagctt ctccttcgcg cgtgtgaacg ccacatagag cacgcggagc tcctcgccca
     5941 gattctccag ttcgatctgc tttgcgatgg cgcgctttaa tatggtcggc gtcttgatcc
     6001 tgcgctcgcc gtcgatccag tcaattccga ttcccagttc cgggtgcagc accatgcggc
     6061 ttctggtatc ctgcttgtta aaatttcttc ccagaccgca cacaaagacc accgggaatt
     6121 ccagtccctt gctcttgtgg atactcataa tgcgcacgac gtcctcattc tccccgacca
     6181 tgtccgcctc gccaaaatcg acatcgtatt tctggagttc atcgatgtag cgcacaaaat
     6241 gaaacaatcc cttgtagctc gtattctcat aggcgatcgc cttctccacc agcatgcgca
     6301 gattcgctct gcgtctggct cctgccggca tcgccgccgc ataatttccg tagccgcttt
     6361 ccttcaacac gatctcgatc agctcgtgaa tcggggtgtc cggcacaagc gcccgcagcc
     6421 tctcatacag cttccagaac tcccacagtt ttttctcata accttccgcc aaaggttctt
     6481 cactctgcgc aagttccctg cagcgtgcga gcacacactc acagaaaggc acgctgcggt
     6541 cgtgcgccct aagccgtccc agctcctcgt ccgtaagccc ggcgatcgga g
//
LOCUS       DBMDQJ010000031         6501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4866_length_6501_cov_1.791094, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000031 DBMDQJ010000000
VERSION     DBMDQJ010000031.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4866_length_6501_cov_1.791094"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1643)
                     /locus_tag="ACZO9R_01165"
     CDS             complement(<1..1643)
                     /locus_tag="ACZO9R_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HIR0911715.1"
                     /translation="MDIIAKISEELGIKRHQTEAAVKLIDEGNTIPFIARYRKEATGA
                     LNDEVLRNLSERLTYLRGLEERKETVLASIEEQGKLTDELKAQILSAETMVLLEDLYR
                     PYKPKRRTRATIAKEKGLEPLAGVITLQMIKTPLIEEAAKYVDAEKGVTTAEEAIAGA
                     SDIIAESISDEADYRTHIRDLTVKKGRMTSTAKDPETESVYEMYYDFDEPVAKLAGHR
                     ILAVNRGEKEKFLTVKIEAPQEDILRYLEKKVIVRDNPQTNEVLRDVVRDAYDRLIAP
                     AIEREIRSNLTERAEDGAIRVFGKNLEQLLMQPPIAGQVVLGWDPAFRTGCKLAVVDP
                     TGKVLDTTVIYPTAPQNKVAEAKAVLKKLIAKYHITLISLGNGTASRESEQIIVDLLK
                     ELPVKVQYIIVNEAGASVYSASKLATEEFPNFDVGQRSAASMARRLQDPLAELVKIDP
                     KSIGVGQYQHDMNQKKLGEALGGVVEDCVNKVGVDLNTASASLLEYVSGISKTLAKNI
                     VTYREENGRFVSRAGLLKVPKLGPKAYEQCAGFLRIGDG"
     gene            1807..2145
                     /locus_tag="ACZO9R_01170"
     CDS             1807..2145
                     /locus_tag="ACZO9R_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911716.1"
                     /translation="MNNKRRSQRMENAALLLGVIGAATFCLVYPALICGSLAIVFALL
                     SRGGERTLTSRAKIALILGAIALGIVLAMIIYTFAIAELYYGGVENMARQVYGTLGID
                     FDTLMNSLGQ"
     gene            complement(2134..2577)
                     /locus_tag="ACZO9R_01175"
     CDS             complement(2134..2577)
                     /locus_tag="ACZO9R_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HIR0911717.1"
                     /translation="MEIGMFQTDRRVDRDFYIMGWCAAGIFAALFLFVRVTGIPVWKF
                     LLPCPFHALTGAYCPGCGGTRAVRALLAGKWLRSFVLHPFVPYAALLSGWFMISQTIE
                     RVSGSRIRIALRFRDVYIWIALLLIVVNCLVKNLALFVWHVDLLA"
     gene            2879..3652
                     /locus_tag="ACZO9R_01180"
     CDS             2879..3652
                     /locus_tag="ACZO9R_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HIR0911718.1"
                     /translation="MSRSSSELKRISRDILNNRYSVPMAAFLTASLIPTLIEIPFSMT
                     LGDYPGTPQLIISTIADILILLIAQMLDTGVMLVHMNMTRGQTYRIRDVFTPFRNGAE
                     RFFLAAVLFDVFLVIAGIPAIAGVLYFYKTGVSGLSGALLAAGSILGLIFTFCVLLTY
                     RMVFFFLLDHPHLSVRDAFRTCRKFMRGRRRKLLYILFSFLGWGALAICSFGIAALWI
                     SPYMTQTLLTFYLDGTGELDQIPVRDYDQEARRFTGSIF"
     gene            3735..5141
                     /locus_tag="ACZO9R_01185"
     CDS             3735..5141
                     /locus_tag="ACZO9R_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HIR0911719.1"
                     /translation="MNEKDGAIRDANVLGVPKMLILGLQHMFAMFGATILVPILVNSY
                     FGEDVLHVQVTLICAGIGTLFFHLCSKFKVPAFLGSSFAFLGGFATIANMDSGVFADM
                     SNADKLSYACGGIVVAGLLYLILALIIKLVGVKKVMRFLPPVVTGPIIICIGLSLAGS
                     AINNASTNWLLALIALVTIIVFNIWGRGMFKIIPILMGVIISYIAALIFNAMGMTNAD
                     GSAILSFTDIANASWVGVPAFSLCKFNLSAILVMAPIALATMMEHIGDISAISATVDQ
                     NFIEDPGLHRTLIGDGLATSLSALVGGPANTTYGENTGVLELSKVYDPKVIRIAACYA
                     IILSFIPKMAAVIGSMPSAIIGGVSFMLYGMISAIGVRNVVENHVDFTQSRNLIIAGV
                     IFVCGLGFSDGLTFTIAGTSVTLTALAVAAIAGIFLNAILPGNDYHFGKDITGDANRG
                     IDMKPNLHEDTSYTDTEE"
     gene            complement(5722..6414)
                     /locus_tag="ACZO9R_01190"
     CDS             complement(5722..6414)
                     /locus_tag="ACZO9R_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF transmembrane domain-containing protein"
                     /protein_id="HIR0911720.1"
                     /translation="MKSSKVFWGIFFILAAVYVVISKFGILPDIGVFSIILTVFFLWL
                     FVEGIRNVNFWEILFSIAFICIIYDEPLGITALTPWTVLGAAFLGSIGLSLIFGGKKR
                     KWKTVTGNVGTSSSEQYTGEEIRCENSFGSAIRYINSDNFRRAAIENNFGSLSVYFDN
                     AIVQEGSANVSVENNFGETNLYIPKEWKIQNNLDRSFGAIRETGSSIGTSSTVLYLNG
                     SANFGEIHLHYI"
BASE COUNT         1440 a         1837 c         1486 g         1738 t
ORIGIN      
        1 tttccgtcgc cgatccgcag gaatcctgcg cactgttcat acgccttcgg tcccagcttc
       61 ggcaccttca aaagtcctgc acgggataca aatctgccgt tttcctcgcg gtaggttaca
      121 atattttttg ccagcgtctt gctgatgccg gagacgtatt ccagaagcga tgccgatgca
      181 gtattcaaat ccacgccgac tttgttcacg caatcctcga ccacgccgcc aagtgcttcg
      241 cccagctttt tctggttcat gtcatgctga tactgtccca caccgatgga cttcggatcg
      301 attttaacaa gctccgccaa cggatcctgc agacggcgcg ccatggacgc tgcgcttcgc
      361 tgtcccacgt caaaattcgg gaattcctcc gtggcaagct tgctggcaga atagactgac
      421 gcgcccgcct cattgacaat aatgtactgt acttttaccg gaagttcctt taacaggtcc
      481 acaatgatct gttcggattc ccgcgatgcc gttccgtttc caagggaaat cagggtaatg
      541 tgatatttgg caatcaattt ctttaatacc gcttttgcct ccgccacttt attctgcgga
      601 gccgtcggat aaatcaccgt cgtatcaagc acttttccgg tcggatctac cacagcaagc
      661 ttacagcctg tccgaaacgc cgggtcccat ccgagcacca cctgtccggc aatcggcggc
      721 tgcatcagca gctgctctaa gttctttccg aatacacgga tggcgccgtc ctccgcacgc
      781 tcggtcaggt tactcctgat ctcgcgctcg atcgccggtg caatcagacg gtcataggcg
      841 tctctcacca catcgcgcag cacctcattc gtctgcgggt tgtcccggac aatcaccttt
      901 ttctccagat agcgcagaat gtcctcctgc ggcgcttcga tcttcacagt gaggaatttt
      961 tctttttctc cgcggttcac cgccagaatc cggtgtccgg caagctttgc caccggctcg
     1021 tcaaaatcgt aatacatctc atagacggat tccgtctccg ggtctttggc agtagaggtc
     1081 atccggcctt ttttcaccgt gaggtcacgg atgtgcgtac ggtaatccgc ctcgtcagag
     1141 atggactccg caataatatc cgacgctccc gcaatcgcct cctcggcggt tgtaactccc
     1201 ttttcggcat ccacgtattt tgccgcctct tctatgagcg gcgtttttat catctgcagt
     1261 gtaatcacac cggcaagcgg ttccagtccc ttttccttgg cgatcgtggc gcgcgttctg
     1321 cgcttcggct tgtacgggcg gtataaatcc tccagcagaa ccatcgtctc cgccgaaagg
     1381 atctgcgctt tcagttcgtc cgtcagcttc ccctgctcct caatgctggc gagcaccgtc
     1441 tccttgcgct cctccagtcc tcttaaatag gtaaggcgct ccgacaggtt acgaagcacc
     1501 tcatcgttta atgcgccggt cgcctccttc cggtaacgcg caataaaagg aatggtattg
     1561 ccttcatcga tcagcttgac tgctgcctct gtctgatggc gcttaattcc cagttcctca
     1621 gaaatttttg caataatatc catatctgtc ctccaaattt taccgagtct gctcccagta
     1681 taccgaaaat ccgctgtaat ctcaagtgcc ttcctcattg tctttttggc tttcccatgt
     1741 tacaatagac aaagttatcc tgcgatatac tggcaatacc ggcatatcgg gaaaggtctg
     1801 attatcatga acaacaaacg acgttcccag cgaatggaaa atgccgctct gcttctcggc
     1861 gtgatcgggg cagctacttt ttgtctggtc tatccggcac tgatctgcgg atctcttgcc
     1921 atcgtttttg cacttttatc ccggggcggc gagcgcacgc tgacctcccg cgcgaaaatt
     1981 gctctgatcc ttggcgccat tgccctgggc attgtcctcg ccatgattat ttacacattt
     2041 gcgattgccg aattatatta tggcggcgtg gaaaatatgg cgcgtcaggt atacggcacg
     2101 ctcggcatcg attttgatac actgatgaat tccctaggcc agtaaatcca catgccacac
     2161 aaacagggca agatttttga ccaggcagtt gaccacgatc aaaagcaggg cgatccatat
     2221 atagacatca cgaaagcgca gggcaatccg tatccggctt ccgctcacgc gttcaatggt
     2281 ctggctgatc ataaaccagc cgctaagcag cgccgcatac ggcacaaacg ggtgcagcac
     2341 aaaggagcgc agccatttgc cggcaagcag cgccctgacg gctcttgtcc cgccgcaccc
     2401 ggggcagtac gctccggtca gcgcatgaaa cgggcagggc agcaaaaatt tccagaccgg
     2461 aattcctgtt actctgacaa acaaaaacag cgccgcgaaa attcccgccg cgcaccagcc
     2521 cataatataa aaatcccggt ctacccgtct atccgtctga aacattccga tctccattcc
     2581 tatcttactc cctgctatta taaccgccgc attttcggtt gtaaagcacg cgcacgtttc
     2641 tggattccaa atctctttaa ctgccagtag catatcacgg tcttccatgc tataatgttc
     2701 gcgaaggcaa aagtgaacat gtatcgaagc cgaatgcaga tgcttgcagc tgcgggcggc
     2761 ttcggtgcat gtgaacgcgc ccgcgagtct gggaaaccga aggttttcca gacttttgcc
     2821 gaagcgcaaa aacctttacc aaaccataca caaatatcac atccacagga gactgcacat
     2881 gtcaagatca tccagcgaat taaaacgtat ttcacgcgac attttaaata accgttattc
     2941 cgtaccgatg gcagcatttc tgactgcctc cttaattccg acgctcatcg agattccgtt
     3001 ctccatgacg ctcggcgatt atcccggcac tccgcagctt atcatcagca cgattgcaga
     3061 tattttaatt cttctgatcg cccagatgct agacaccggc gttatgctgg ttcatatgaa
     3121 tatgacgcgc ggtcagacat accgcatccg tgatgttttt acaccgtttc gcaatggcgc
     3181 agagcgcttc tttctcgctg ccgttctctt cgatgtattt ctggtgatcg caggtattcc
     3241 tgccattgcc ggtgttcttt atttttataa aaccggtgtc tccggtctct ccggtgccct
     3301 gcttgctgcc ggaagcatcc tcggtctgat ttttactttc tgtgtgttgc ttacctaccg
     3361 catggttttc ttctttcttt tagaccatcc gcatttaagc gtgcgggatg cttttcgcac
     3421 ctgccgtaaa tttatgcgcg gacgacgcag gaagctgctt tacattttat tcagctttct
     3481 cggctggggc gcgcttgcca tctgctcttt tggcattgcg gctctctgga tcagtcctta
     3541 tatgacgcag acgctgctca ctttctatct cgacggtacc ggcgaactgg accagattcc
     3601 cgtccgtgat tacgatcagg aggcaagacg tttcaccgga tctatttttt gatatcttat
     3661 ggcaaatcag aagttctgat ttgccatttg tatatacgca actaccaatc atttcacatc
     3721 aggaggagtt tactatgaac gaaaaagacg gagcaatccg cgacgccaac gtacttggcg
     3781 taccaaaaat gctgattctt ggcctgcagc atatgtttgc catgttcggc gcaaccattc
     3841 ttgtaccaat tcttgtcaac agctacttcg gcgaagatgt tctacatgta caggttacat
     3901 tgatctgtgc cggtatcgga actttatttt tccatctatg ttccaaattc aaggttccgg
     3961 cgtttctcgg ttcttccttt gccttcctcg gcggttttgc cacgattgca aacatggatt
     4021 ccggtgtatt cgccgacatg tccaacgctg acaaactttc ctatgcctgc ggtggaattg
     4081 ttgtcgcggg tcttttatat ctgattctcg cgctgattat caagcttgtc ggtgtcaaga
     4141 aggtcatgcg ttttcttccg ccggtcgtaa ccggtccgat catcatctgc atcggactga
     4201 gccttgccgg ttctgccatc aacaacgctt ccaccaactg gcttctcgcc ctcatcgctc
     4261 tggttacgat cattgttttc aatatctggg gcaggggaat gtttaagatc atcccgattc
     4321 tgatgggcgt cattatttct tatattgccg cattgatctt caacgccatg ggcatgacaa
     4381 acgcagacgg ctccgccatt ctttctttta ccgacatcgc aaacgcttcc tgggtcggcg
     4441 taccggcatt ttccctctgc aaattcaatc tgtccgccat tttggtcatg gccccgatcg
     4501 cactcgcaac catgatggaa cacattggcg atatttccgc catctccgcg acggttgatc
     4561 agaattttat cgaagatcca ggacttcacc gcaccctgat cggtgacgga ctcgccactt
     4621 ccctgtccgc tctcgtcgga ggtcccgcaa acaccaccta cggcgaaaat accggcgtgc
     4681 tcgagctgag caaggtatac gatccaaagg tcatccgcat cgcagcctgc tacgccatca
     4741 tcttaagctt tattccgaag atggctgctg tgatcggttc catgccgtcc gctatcatcg
     4801 gcggtgtcag cttcatgctc tacggtatga tctccgccat cggcgtccgc aatgttgtgg
     4861 aaaatcatgt ggattttacg cagtcacgca atctgatcat cgcaggcgtg atcttcgtgt
     4921 gcggtcttgg tttctccgac ggtcttacct ttacgatcgc cggaacctcc gtcacactca
     4981 ccgcgctggc agtcgcagcg atcgcaggta ttttcttaaa tgccatcctg ccgggcaacg
     5041 actatcactt cggaaaagac atcacaggtg atgcgaaccg cggtattgac atgaagccga
     5101 accttcacga agataccagc tacacagata cagaggaata aacataccgc caaaagcgga
     5161 ggcggcaggg aacagtgccc ggatgatgta aaagttttct gacgtttcag gatgcaaccg
     5221 gagacggtca ttctgatcgc aaaatccggt atttctaatc cgcaccgcgt ttgacatcct
     5281 cgatgatccg tgtctttcca agttttgagc actccgcgaa atgtggccgc actccgcaga
     5341 caatttaagg gctttcgtgt gtgccccttg tcgacaccgc taaacaaaac ataaccgcgc
     5401 gaagccgatg tctgagttgg catctgccct attttcaata gcagatgcca gcgagtttcg
     5461 gttcgcgcgg ttttatattg tctgtgaagc ggtgccgcta ggggcaccag gaagtccgct
     5521 cattgtctgc ggcgtgacgg acacatcccg cggattgtca aaacgtggcc aagacacaga
     5581 atccgatgtc aaacgcagtg tggaagaaat atagggattt tgctcaaaag aatgaaacgt
     5641 ctccggctgc actctgaaac gctgtaaacc tttttacatg atgcccgcga tccctgctgc
     5701 ttccactttc cgcggtattt cttaaatgta gtgcagatga atctctccga agttcgcgga
     5761 gccattcaga tacagcaccg tgctgctcgt tccgatgctg ctgccggttt cacggatggc
     5821 gccgaacgaa cggtccagat tattctgaat cttccattct ttcgggatgt aaaggttggt
     5881 ctccccgaaa ttattctcca cgctcacatt cgcagagcct tcctgcacga tcgcattgtc
     5941 gaagtacacc gaaagcgatc cgaaattatt ttcgatggcg gcgcgtctga agttgtcgga
     6001 gttgatgtag cggattgcgg aaccgaagct gttctcacac cggatctctt cgccggtata
     6061 ctgttcactg ctggatgtcc cgacattgcc ggtcaccgtc ttccatttcc gctttttccc
     6121 gccgaaaatg agtgacagac cgatgcttcc gaggaatgcc gcgccgagca ccgtccacgg
     6181 cgtgagcgct gtaattccaa gcggctcatc gtatatgata cagataaacg caattgagaa
     6241 caggatttcc cagaaattca cattgcggat tccctcgaca aaaagccaga ggaaaaatac
     6301 ggtcagaata atgctgaaca cgccgatatc cggcagaatt ccaaatttgc ttataacgac
     6361 atacactgct gcgaggataa aaaagattcc ccagaatact ttgctacttt tcatgatgca
     6421 acctcttttc ttctaatttt tgagtcaacg gtttatagta ataccgcgat acatagacct
     6481 tcttgtgcgt gttcaaaaac g
//
LOCUS       DBMDQJ010000032         6470 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4884_length_6470_cov_1.323285, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000032 DBMDQJ010000000
VERSION     DBMDQJ010000032.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4884_length_6470_cov_1.323285"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            320..1300
                     /locus_tag="ACZO9R_01195"
     CDS             320..1300
                     /locus_tag="ACZO9R_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent 4,6-dehydratase LegB"
                     /protein_id="HIR0911721.1"
                     /translation="MKKVLVTGADGFIGSHLTESLLERGYDVKAFVYYNSFNTWGWLD
                     TLPKEKLDQIEIFAGDVRDPNGVREAMRGVDGVFHLAALIAIPFSYHSPDSYVDTNIK
                     GTLNVLQAARELQTERVMVTSTSEVYGTAQYVPIDEKHPFQGQSPYSATKIGADRLAE
                     SFYRSFQLPVAIVRPFNTYGPRQSARAVIPTIITQLLAGKEEIQLGSLTPTRDFNYVK
                     DTAAGFIAIAESEKTIGQEINIATQQEITIGELAQEIIGQINPGAKIICDEQRLRPEK
                     SEVNRLLGSNAKLKELTEWKQRYTFEQGIAETIEWIRQHMDVYKTDIYNI"
     gene            1324..2508
                     /locus_tag="ACZO9R_01200"
     CDS             1324..2508
                     /locus_tag="ACZO9R_01200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LegC family aminotransferase"
                     /protein_id="HIR0911722.1"
                     /translation="MKRFVPLSIPNFNGNEKKYVDDALEQGWVSTGGAYITRLEQQLA
                     EFLKVDKAAACQSGTSGLHLALVECGVQPGDMVIVPTLTFIAAVNPVRYQFAEPVFMD
                     CDESLCLDPEKLAEFCEKECRLEAQQLVHKKSGRVVKAVIVVHVFGNVADMEAIMSIA
                     GKYHLKVVEDATEALGSHYTAGKLAGRYAGTIGDFGVYSFNGNKIITTGGGGAVTARC
                     ADEVEHLKYLSTQAKDDPQFYIHNEIGYNYRMTNLQAALGVAQMEELQQFIETKHRNY
                     ILYKERFQDASFGTLLPFREGTCSNQWFYSLQLREDRLQGKDMRDIIGALQERGVQTR
                     AIWGLIHEQKPYRQNIAYKIEKAPCYSATVLNIPSSTQLTEEDICYAAEQITEVLGGD
                     EG"
     gene            2529..3572
                     /locus_tag="ACZO9R_01205"
     CDS             2529..3572
                     /locus_tag="ACZO9R_01205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR0911723.1"
                     /translation="MKKNVLLFSHQMKVGGAPTVLCEMGKILSKKYNVVVLSPSDGAM
                     RTQFEEAGISVLISGEITDALRDMIVQEFDFAVANTTLALPFVVKLAGRMPVYWWIHE
                     NTLAYEGRQEMKEYYQFAAKYARIFSAGHVAAENFYHFYRIKTEILEFGLSDCYVAAR
                     DNRKRDHINVICPSTVCELKGQDILVQAIKSMDAGLREKCKFVFLGELRNDNAKEYQM
                     IETLAKEYQNVELHPLVEREKLLELYYDMDVVVAPSREDATPATIVEGLMYHKICIGS
                     SGTGVSRYLKDGWDGIVFENANTDDLRNKLEYMVREFADLGELRERGRKVYETIYSYD
                     IFEKNVLEKIGTV"
     gene            3636..4778
                     /locus_tag="ACZO9R_01210"
     CDS             3636..4778
                     /locus_tag="ACZO9R_01210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0911724.1"
                     /translation="MNLSICIITKNEEQNIDRCLKALAPYGLETIVVDTGSTDRTKQI
                     AARYTDRLYDFPWCDDFSAAKNFAIEKASHSYVLVLDSDEFAEQLDLSVLEKQIAAHP
                     GAVGRIRRRNVFHRNGEEQENREWINRIFAKEYFHYEGRIHEQVMPLHGAESGYQTYE
                     TPVVILHTGYDLPEQQKKEKAKRNIRLLEQELKQLGWDENAGAKDGVAKAETAGTDAN
                     ARGSGQIPYLLYQLGKSYYMLGEYGVACEWFAQGLSFDLNPELEYVIDMVETYGYALI
                     NSGQEQTALFFENIYDEFGKSADFQFLMGLIYMKNARFTEAVREFEKAAGHAECRAQG
                     VNSYRADYNIGVIYECLGKKEEALAYYRKCGSYAPAEERIRELGRR"
     gene            4790..5116
                     /locus_tag="ACZO9R_01215"
     CDS             4790..5116
                     /locus_tag="ACZO9R_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR0911725.1"
                     /translation="MTTREEALEYGMSFPDVYVDTPFHDPNWVLVRSRKNKRAFLWTY
                     EYQGQMRINVKADPEWIDFWRKAYEAVIPGYHQNKKHWNTVILDGSVPDGDVKRMIAE
                     SYDLVR"
     gene            5200..5376
                     /locus_tag="ACZO9R_01220"
     CDS             5200..5376
                     /locus_tag="ACZO9R_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911726.1"
                     /translation="MTGIEALSVMGLALLAVGILSGLKAYMQSVGKAALQAILGAGTS
                     EVVKELLTLVLRKG"
     gene            5571..>6470
                     /locus_tag="ACZO9R_01225"
     CDS             5571..>6470
                     /locus_tag="ACZO9R_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0911727.1"
                     /translation="MTLQFPTHGLIIGAVRGGHAMQYMTSRQKATEWSVTKRMVNYWC
                     ANGQIEGAYKEGSRWWIPVDVERPGEEECLRRMRAYTVRITGKKSVAVGIQDFEALRR
                     DQMFYVDKTDFIRQWWESGETTTLITRPRRFGKTLNLSMLNCFFSVSYENRADLFEGL
                     KVWEEKSYHKLQGRFPVIFLSFAGVKGTTFEAVLRQINYGIVEVYRRFERILDMSRFT
                     ERERQDFGRISWDMDASTAAQSVRLLTDLLYVYYGHKPIILLDEYDTPLQEAYFNSFW
                     DEMVSFVGAFFNNSFKTNPSLGRA"
BASE COUNT         1871 a         1138 c         1904 g         1557 t
ORIGIN      
        1 gcagaacata gcgaggtaat agatgcttac attgaaagaa tgtataaaaa ggggacagag
       61 actgttaatt catataagat aagtccacaa atgctgttga agtgtcagaa aaatgtcagg
      121 aaaatgtgcg ggatacttat ggaagagatt agttagtgag aagaaaaaga ttttgttgtt
      181 tcatggagaa tggaagtgca ggaaattgta aaaagaatca atgggaacat tcatacatct
      241 gggtgtaatt taaaagtagt tactgttatc tggaatgtgg aaattatgaa cctgcaatac
      301 aacagttaga gggattgaga tgaaaaaagt acttgtgacg ggagcggacg gatttatcgg
      361 gagtcattta accgaaagct tattggagag aggctatgat gtgaaggcat tcgtgtatta
      421 taattcgttt aatacatggg gatggcttga tacactgccg aaggaaaagc tggatcagat
      481 agagattttt gcaggagacg tgagagatcc caatggtgtt cgggaggcga tgcggggggt
      541 agacggcgta tttcacttgg cggcgctgat tgcaattccg tttagctacc attcgccgga
      601 ttcttatgtg gatacgaata ttaagggaac gctcaatgtg ctgcaggcgg cgcgtgaact
      661 gcagacagaa cgcgttatgg tgacatccac atcggaggta tatggaacgg cgcagtatgt
      721 accgattgat gaaaagcatc cgtttcaggg acagtcgccg tattcggcaa ccaagattgg
      781 agccgaccgg ctggcggaga gtttctatcg aagctttcag ctgccggtgg ccatcgtcag
      841 accgtttaat acgtacggcc cgagacagtc cgcgcgggcg gtgattccga cgattattac
      901 acagctgctg gcaggaaagg aagagattca gcttggttcc ctgaccccta caagagattt
      961 taactatgta aaagatacag cggcaggatt tattgcgatc gcggagtctg agaagacaat
     1021 tggtcaggag atcaacattg caacgcagca ggagattacg atcggagaac ttgcgcagga
     1081 gattatcggg cagatcaatc ccggggcaaa aattatatgt gatgaacagc ggcttcgacc
     1141 ggaaaagagc gaagtaaatc gtctgctggg atcaaatgca aagttaaagg aattgacgga
     1201 atggaagcag agatatacgt ttgagcaggg gattgcggag acgatcgagt ggatcagaca
     1261 gcacatggat gtttacaaaa cagatattta taatatatag atcggacaga agagaggaag
     1321 agaatgaaac ggtttgtgcc attatccatt cctaacttta acgggaatga aaaaaaatat
     1381 gtagatgacg cattggaaca gggctgggtg tctacaggcg gcgcttatat taccaggctg
     1441 gaacagcagc tggcagaatt tttgaaggtg gacaaggctg ctgcctgcca gagtggcaca
     1501 tccggactgc acctggcact cgtggagtgc ggtgtgcagc caggagacat ggtgatagtt
     1561 cctactttaa cctttattgc tgcggtaaat ccggtgcgct atcaattcgc agaacctgtt
     1621 tttatggact gtgatgagag tctgtgcctt gatcctgaaa aattagcgga gttctgtgag
     1681 aaagaatgca gactggaggc gcagcagctg gtacataaga agagcggcag agtggtaaaa
     1741 gcggtcattg tggtacacgt attcggcaat gtcgccgata tggaagcaat catgagtatt
     1801 gccggaaaat atcatctgaa ggtagtggaa gatgccacgg aggcattggg atcacattat
     1861 acggcgggaa agcttgcggg gcgatatgcg gggacgattg gtgatttcgg cgtgtattcc
     1921 ttcaatggaa acaaaatcat tacgaccgga ggtggaggcg ctgtcactgc gagatgtgca
     1981 gatgaagtgg aacatctgaa atatctctct acacaggcaa aggatgatcc gcaattttac
     2041 atccataatg aaattggata caattatcgt atgacgaatc tgcaggcggc gcttggcgtt
     2101 gcgcagatgg aggaactgca gcagttcata gagacaaaac acaggaatta tattctgtat
     2161 aaggaacgat tccaggatgc ttcttttgga acactgctcc cgtttcgcga gggaacatgt
     2221 tccaatcagt ggttttattc acttcaatta cgggaagacc ggctgcaggg aaaggacatg
     2281 cgcgatataa tcggggcttt acaggaacgt ggggtacaga cgcgtgcaat atgggggctg
     2341 attcatgaac agaagccgta ccggcagaat attgcttata aaatagagaa agcaccctgc
     2401 tacagtgcga ctgttttaaa tattccgagc agcacgcagc tgacagaaga ggatatttgt
     2461 tatgctgcgg agcagataac ggaagttttg ggcggagacg aagggtaatc tttcattgga
     2521 gagactttat gaaaaagaat gtattgttat tttcgcatca gatgaaggtg gggggcgccc
     2581 cgacggtatt gtgcgaaatg ggaaagattt tgagcaaaaa gtataatgta gtagtattat
     2641 ccccgtcgga cggagcgatg cggacgcagt ttgaggaagc ggggatatcc gttcttataa
     2701 gcggagaaat aacggatgca ttacgcgata tgattgttca ggagttcgat tttgcagtgg
     2761 caaatactac gctggcgctc ccgtttgttg tgaaactggc aggcaggatg ccggtttatt
     2821 ggtggataca tgaaaatacg ctggcctatg aaggacgcca ggagatgaaa gagtattacc
     2881 agtttgcggc aaaatacgcg aggatttttt ctgccggaca tgttgcggca gaaaattttt
     2941 atcactttta caggataaag acggaaatat tggagtttgg tctgtcagat tgctatgtgg
     3001 cagccaggga taaccggaag cgtgaccata tcaatgtgat ctgtccttcg acagtctgcg
     3061 aattgaaggg gcaggacata ctggtgcagg caataaagag tatggatgcc gggttgcggg
     3121 aaaaatgcaa attcgtattt ctgggtgaac ttagaaacga taatgcaaaa gaatatcaga
     3181 tgatagaaac acttgcaaaa gaatatcaaa atgtagaact tcatccgctc gtggaaagag
     3241 aaaaattact ggagctgtat tatgacatgg atgtagtggt ggcgccatcc agagaggacg
     3301 ccacgccggc taccatagtg gaagggctta tgtatcacaa aatatgcatt ggttccagcg
     3361 gcaccggtgt atccagatat ttaaaagacg ggtgggatgg tattgtattt gaaaatgcaa
     3421 acacagatga cctgaggaat aagctggaat atatggtgcg ggaatttgct gatttagggg
     3481 aacttagaga acggggaaga aaggtttacg aaacaattta cagctatgat atttttgaaa
     3541 agaatgtcct ggaaaagata ggaaccgttt gagggaagcc ggcagataag cggccaggat
     3601 gagtcggcga acttatagta tgaaaggtac gtgatatgaa cctatccatc tgtatcatta
     3661 caaaaaacga agaacagaat atcgaccgat gcttaaaggc gcttgctccg tacggtctgg
     3721 agacgattgt ggtggatacc ggatcgacgg accgcacgaa acagattgcg gcgcgttata
     3781 cggacaggct gtatgatttc ccgtggtgcg acgatttttc ggcggcaaag aattttgcga
     3841 tagaaaaggc ctcacattcc tatgtactgg ttttggacag cgacgagttt gcagagcagc
     3901 tggatctgtc cgtactggaa aaacagattg ccgcgcatcc gggggcggtc ggccggatcc
     3961 gcaggcggaa tgtgtttcac agaaacgggg aggaacagga gaaccgggag tggatcaacc
     4021 gcatattcgc aaaagaatat tttcattacg aaggaaggat tcatgagcag gtgatgcccc
     4081 tgcacggcgc agagagcggt taccagacgt atgagacgcc ggttgtgatc ttgcacaccg
     4141 gatacgatct gcccgagcag cagaaaaaag aaaaggcaaa gcgcaacatc cgcttgttgg
     4201 agcaggaatt aaagcagctg ggctgggatg aaaatgccgg ggcaaaagac ggcgtcgcaa
     4261 aggcagagac agccggaaca gatgcgaatg ctcgtggaag cgggcagatt ccttatcttc
     4321 tgtatcagct cggcaagagt tattacatgc tgggagaata cggcgtggcg tgcgaatggt
     4381 tcgcgcaggg gctttcgttt gacctaaatc ctgaactgga atacgtgatt gacatggtgg
     4441 agacatacgg atatgcgtta atcaacagcg ggcaggagca gacggcactg ttttttgaga
     4501 atatctatga cgaatttgga aaaagtgcgg attttcagtt cctgatggga ttgatttaca
     4561 tgaaaaacgc acggttcaca gaggcggtgc gcgaatttga gaaggcggcc gggcatgcag
     4621 agtgccgggc acagggagtg aattcctatc gggcggacta taacatcggc gtcatctacg
     4681 agtgcctcgg gaaaaaggaa gaggcgcttg cctattaccg gaagtgcggc agctatgcgc
     4741 cggcagagga gcggatacga gagctggggc ggaggtagaa gaaaaagcta tgacaacgag
     4801 agaagaagca ttggaatacg gaatgtcatt tccggatgtc tatgtggaca caccgtttca
     4861 cgatccaaac tgggtgctgg tgcgttcccg gaaaaataag cgggcatttc tctggactta
     4921 tgaatatcag ggacagatgc ggattaatgt caaggcagat ccggagtgga ttgatttctg
     4981 gcggaaagcg tacgaagcag tcattccggg ttatcatcag aacaaaaagc actggaatac
     5041 ggtcatcctt gacggcagtg tgccggatgg agatgtaaag cggatgatag cggaaagcta
     5101 cgatctggtt aggtaagggt agtgtcgtgc tcgtgcatgg atatatgagg ggatgcaaat
     5161 agagttgtat ttgaggctac agttggattt ctactatgtt tgacagggat cgaggcgtta
     5221 agtgtaatgg gactggcatt actggcagtg gggattttat ccggtttaaa ggcttatatg
     5281 cagagcgtag gaaaagcagc acttcaggct attctgggag caggtacatc ggaggttgtg
     5341 aaagagttat tgactctggt gctgcggaaa ggatgatagc gatatcgaaa atgcttaaaa
     5401 gaagatggca gggacaacgg gaaccctgcc attttattcg tgaatatttt ggacagatag
     5461 atcgggttta taatttacat aacagatccg gggtggaagc ctacattaaa tccttgcatg
     5521 tgatataagg ataagaacat ataaggaaat aactgggaag taactgggaa atgacgttgc
     5581 aatttcccac ccacgggctt ataattgggg cagttagggg agggcatgca atgcaatata
     5641 tgacatccag acagaaagct acagaatggt ctgtcacgaa gcgtatggta aattactggt
     5701 gcgcaaacgg gcagattgaa ggtgcataca aagaaggcag ccgctggtgg attccagtgg
     5761 atgtagaacg tcctggagaa gaggagtgtt tgcggcgcat gagagcgtac accgttcgga
     5821 ttacaggcaa gaagagcgtt gcggttggca ttcaggattt tgaggcgtta agacgtgatc
     5881 agatgtttta tgtagacaag acggatttta ttcggcagtg gtgggaatct ggcgagacga
     5941 ctacgctgat tacacgcccc agacgttttg gcaagacgtt gaatctgagc atgctgaact
     6001 gctttttttc cgtgtcctat gaaaatcgtg cggatttatt tgaggggttg aaggtttggg
     6061 aagagaaaag ctatcacaag ctgcaagggc gtttccccgt tatatttctg tcatttgcag
     6121 gtgttaaggg gacaacgttt gaggcggtgc ttcggcagat aaattatggt attgtagagg
     6181 tatatcgccg ctttgagcgg atacttgaca tgtcgcggtt cacggaaagg gaacgacagg
     6241 attttggcag aatatcatgg gacatggatg cgtccacggc tgcgcagtcc gtacggcttc
     6301 tgacggattt gctctatgtg tattatgggc ataagcccat tattttactg gatgaatatg
     6361 atactccgct tcaggaagct tactttaata gcttttggga tgagatggtt tcttttgtgg
     6421 gggctttttt taataattcc tttaaaacga atccatcgct ggggagagca
//
LOCUS       DBMDQJ010000033         6440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4914_length_6440_cov_1.478151, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000033 DBMDQJ010000000
VERSION     DBMDQJ010000033.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6440
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4914_length_6440_cov_1.478151"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="ACZO9R_01230"
     CDS             <1..218
                     /locus_tag="ACZO9R_01230"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741982.1"
                     /note="catalyzes the reaction of glycine with
                     5,10-methylenetetrahydrofolate to form L-serine and
                     tetrahydrofolate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR0911728.1"
                     /translation="ANKNTIPNDPQSPFVTSGIRLGTPAVTSRGLNTEDMDQVAEAIA
                     LVIKGGEEQVPVARAIIQKLTDKYPLI"
     gene            complement(280..840)
                     /locus_tag="ACZO9R_01235"
     CDS             complement(280..840)
                     /locus_tag="ACZO9R_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HIR0911729.1"
                     /translation="MDKYITMPITEETTAELKAGDYVYLTGTMYVARDAAHKRMIEAL
                     DRGEELPIDIKDATIYYMGPSPAREGRPIGSAGPTTATRMDKYAPRLLDLGEKAMIGK
                     GKRSKEVIDAVIRNHAVYFAAIGGAGALLSKCITKSEIVCYEDLGAEAIRKIEVKDFP
                     VIVVIDREGNNLYETAIRQYHTLEEM"
     gene            complement(948..1763)
                     /locus_tag="ACZO9R_01240"
     CDS             complement(948..1763)
                     /locus_tag="ACZO9R_01240"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005608277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HIR0911730.1"
                     /translation="MIRTIQTDEITKNIKEMCIQANHYLSADTEQSELGKKILHQLQD
                     NLKIADEEMIPICQDTGMAVIFLEVGQDVHFEGTAIEDAVNEGVRQGYTEGFLRKSVV
                     GDPLIRENTKDNTPAVIHYSIVPGDQVKITMAPKGFGSENMSRMFMLKPADGIEGVRH
                     AILTAVKDAGPNACPPMVVGVGIGGTFEKCALLAKQALTRPVDEHSDIPYVKDLEEEM
                     LSKINQLGIGPGGLGGTTTALAVNINTYPTHIAGLPVAVNICCHVNRHVVRTL"
     gene            complement(1802..2965)
                     /locus_tag="ACZO9R_01245"
     CDS             complement(1802..2965)
                     /locus_tag="ACZO9R_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0911731.1"
                     /translation="MPQNFYELVWIFIIYAFIGWCTEVSYAALDTGKFVNRGFLNGPY
                     CPIYGCGVVIVVAILTPLKENLLILFAGSFLLTSVLEYITGYILEKVFHNKWWDYSDK
                     PFNIKGYVCLKFSIYWGLACTFIMDIIHPIIYAAIRFIPFVLGVVLLSIIMCVFAADC
                     IITVTTILKFNKRLKVMDEMAASIHRLSDEIGENIYENVTDVIEKSEKFQETHVELMD
                     KISETKENIYGLPQTAKDKLAETTGAAKDKLAETTGAAKDKLAETTGAAKDKLAETTG
                     AAKDKLSETKESISELPQTAKNKLAEKAENYAASREEKKRERELALEEKKRERAELME
                     RYEKLYEEKSFGFKRLMKAFPDMKSRDNNETLEKFKIHFNIKKKDENKKDQAS"
     gene            complement(3049..3432)
                     /gene="panD"
                     /locus_tag="ACZO9R_01250"
     CDS             complement(3049..3432)
                     /gene="panD"
                     /locus_tag="ACZO9R_01250"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HIR0911732.1"
                     /translation="MTIKMLKAKIHRATVTQAELDYVGSITVDMDLLEQAGILEYEKV
                     QIVDVNNGSRFETYTIAGERGSGVMCLNGAAARMVQTGDKIILMAYAQVTPEEASELR
                     PTVLFVDEKNKVTKVTNYEKHGEIG"
     gene            complement(3436..4284)
                     /gene="panC"
                     /locus_tag="ACZO9R_01255"
     CDS             complement(3436..4284)
                     /gene="panC"
                     /locus_tag="ACZO9R_01255"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR0911733.1"
                     /translation="MKIVSTIEEVRAQVKEWKKEGQSIGFVPTMGYLHEGHMSLIDAA
                     GENDKVVVSIFVNPMQFGPTEDLASYPRDLEHDAKLCEEHGVDLIFHPTPEEMYGDQF
                     YSYVDMDVLTKELCGLSRPVHFRGVCTVVTKLFNIVTPDRAYFGQKDAQQLAVIKRMV
                     KDLNMPLTITGCPIIREADGLAKSSRNTYLSIEEREAALVLSRSIFLGKEMVEKGERD
                     CKKILAAMTAEIEKEPLAKIDYVKIVDLDTMQQVEKIDRGILAAIAVYIGKTRLIDNF
                     MYELEN"
     gene            complement(4313..5140)
                     /gene="panB"
                     /locus_tag="ACZO9R_01260"
     CDS             complement(4313..5140)
                     /gene="panB"
                     /locus_tag="ACZO9R_01260"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR0911734.1"
                     /translation="MKNTVLTLQKQKEEHDKIVMVTAYDYTTAKIMDEAGVNTILVGD
                     SLGMVMLGYEDTLSVTMEDMIHHTAAVSRGAKDAFVVADMPFMSYQTSVYDAVVNAGR
                     LMKEGRANAVKLEGGVEFAPHIEAIVKSSIPVVAHIGLTPQSVNAFGGFKVQGKDVEA
                     AKKLLADAKAVEAAGACMVVMECVPAKLAAKISEELTIPTIGIGAGAGCDGQVLVYQD
                     LLAMYGDFRPKFVKVFANVGEVMREGVKAYIKETKEGTFPGEEHCFKIDDSVIEKLY"
     gene            complement(5192..6094)
                     /locus_tag="ACZO9R_01265"
     CDS             complement(5192..6094)
                     /locus_tag="ACZO9R_01265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR0911735.1"
                     /translation="MNDVQTERMQIGFIGAGKVGVSLGKYFKEKGRNVGGYYSLTRAS
                     AAWAAAFTQTTCYESLEEIISSCGMILFTVPDSAIAEVWQQAKPYVSGKVIGHCSGLY
                     SSDIFSDWDSMNCHACSVHPLAAISSKENAWRELSGVLFTLEGDSSYVKDLETFFYSM
                     GNRTRIISKEDKIKYHAAAAISSNYMTALFSMSEQLLLDCGFAQEEARSELYALAKGN
                     LDHILTQGCVQSLTGPLERNDCATVEKHISALNKKQRKIYTSCAEYLTDLAQSKHPDR
                     DYTEMRKLCRDGKHSLGAECAETV"
BASE COUNT         1434 a         1773 c         1355 g         1878 t
ORIGIN      
        1 ctgctaacaa gaacaccatc cccaacgatc ctcagtctcc gtttgtgacc agcggtatcc
       61 gcctgggaac ccccgcggtt acttccagag gtctgaatac cgaggacatg gatcaggtgg
      121 cagaagctat tgccctggtc atcaagggcg gagaagagca ggttcctgta gccagagcca
      181 tcattcagaa attaacagac aaatatcctt tgatctaaag cttggaggag tataagaaaa
      241 gagccgaaag gctctttttt gttgcaggaa aatgacccgc tacatttctt ctaatgtgtg
      301 atactgtcgg attgctgtct cgtagaggtt gtttccctcg cggtcgatca cgacgatgac
      361 cggaaaatct tttacctcga tcttgcggat tgcctccgcg ccgagatcct cataacatac
      421 aatctcagat tttgtgatgc atttggaaag cagtgcgccg gctccgccga tggctgcaaa
      481 atagacggcg tggttgcgga tcaccgcgtc aatcacttct tttgagcgct ttccttttcc
      541 aatcattgcc ttctcaccga gatccaacag tctcggagcg tatttgtcca tacgggtggc
      601 tgtggtcggt ccggcagatc cgatcggacg accctccctg gcgggagacg gtcccatata
      661 atagatcgtc gcatccttga tgtcaatcgg aagctcctcg ccccggtcca atgcctcgat
      721 catgcgctta tgtgccgcat cgcgcgccac atacatggtt cctgtcagat agacatagtc
      781 cccggctttt aattctgcgg tagtttcttc tgtaattggc attgtaatat acttatccat
      841 aatctttctt tcaaacctcc tgcttatcaa cattagattt tcattttgtg gaaaacctgt
      901 cgttttttat ttttctgcgt gcattacatg ctacatgcac cacgatctta cagggttctc
      961 actacatgcc ggttgacatg gcagcagata ttgacagcta ccggaagtcc cgcaatgtgc
     1021 gtcggatagg tattgatgtt cactgcaagc gccgtcgtcg ttcctccgag tcctcccggt
     1081 ccgattccaa gctgattgat cttcgagagc atctcttcct cgagatcttt gacataggga
     1141 atatcggaat gttcatcgac cggtctcgtc agcgcctgct tggcgagaag cgcgcatttt
     1201 tcaaatgtac cgccaattcc gactccgacg accatcggcg gacatgcatt cggccccgca
     1261 tcctttacgg cggtaagaat cgcatgcctg actccctcga tgccgtctgc cggttttaac
     1321 atgaacatgc ggctcatatt ttcactgccg aatcctttcg gcgccatggt gatttttacc
     1381 tgatcgcccg gcacgatgga ataatgaatg actgccggcg tgttatcttt tgtattttca
     1441 cgaatgagcg gatcaccgac aacggatttg cgcaggaatc cctctgtgta tccctgacgg
     1501 acaccctcgt tgacggcatc ctcaatcgct gtgccctcaa agtggacgtc ctgtccgact
     1561 tccagaaaga tcactgccat tccggtgtcc tgacagatcg gaatcatctc ctcgtccgcg
     1621 atctttaaat tgtcctgcag ctgatgtaaa atctttttcc caagttcaga ctgttctgtg
     1681 tcagcggaaa gataatgatt tgcctgtata cacatttctt tgatattttt tgtaatctca
     1741 tctgtttgaa ttgttcttat cataccgttc ttcctctccc tgtctgctgt gtatgtggcg
     1801 tttaggatgc ctgatctttt ttgttttcgt ctttcttttt gatattaaag tgaatcttga
     1861 atttttccag cgtctcgttg ttatcccgcg acttcatatc cgggaaggct ttcatcagac
     1921 gtttaaaacc aaagcttttc tcctcgtaca gcttctcgta gcgctccata agttctgcgc
     1981 gctcgcgctt tttctcctcg agtgcaagtt cgcgttcgcg ctttttctcc tcacgggaag
     2041 ctgcataatt ctctgccttc tcggcaagct tgttcttcgc tgtctgcggc agttccgaaa
     2101 tgctctcctt ggtctctgaa agcttatcct tcgccgctcc cgtcgtctcc gcaagcttat
     2161 ccttcgctgc ccctgtcgtc tccgcaagtt tatccttcgc tgctcccgtc gtctccgcaa
     2221 gcttatcctt cgccgctccc gttgtctccg caagtttatc cttcgctgtc tgcggcagcc
     2281 cgtaaatatt ttccttcgtc tcgctgatct tatccatcag ctccacatgt gtttcctgga
     2341 atttctcgga tttttcaata acgtctgtca cattttcata gatattttcg ccgatctcat
     2401 cggacagccg gtgaatagac gccgccatct catccatgac ctttaagcgc ttattgaatt
     2461 tcagaatcgt cgtcaccgtg atgatgcagt ctgccgcaaa cacgcacatg ataatactga
     2521 gcagcactac gccaagcacg aacggaataa agcggatggc tgcataaatg atcggatgaa
     2581 tgatatccat gataaaggta catgccagtc cccagtagat cgaaaacttc aggcagacgt
     2641 aacctttgat gttgaatggt ttgtcagaat agtcccacca cttgttgtga aatacttttt
     2701 ccagaatata acctgtaata tactccagca cagaggtaag aagaaaagat cctgcaaaca
     2761 ggatcagcag attttctttc agaggtgtca ggatggcaac cacaatcacc acgccgcacc
     2821 cgtagatcgg acagtacgga ccgtttaaaa atccacggtt cacgaacttt ccggtatcga
     2881 gtgcagcgta ggatacctcg gtacaccaac cgataaatgc atagataata aaaatccaga
     2941 ccagttcata aaaattctgc ggcataatca ttctcctttg tcccgtgctt tctccacgta
     3001 ctcataagaa tcattatagt ccatagaatt ttatgaaaca atcctatatt atccgatttc
     3061 tccgtgtttt tcgtaatttg tcacctttgt gactttattt ttctcatcca cgaaaagcac
     3121 cgttggacga agctctgatg cttcctccgg cgtcacctgc gcgtatgcca tcagaataat
     3181 cttgtcgccg gtctgcacca tgcgtgccgc cgcaccgttt aagcacatca cgccgctgcc
     3241 tcgctctccg gcaatcgtgt aggtctcaaa gcgggagccg ttgttgacgt ccacgatctg
     3301 taccttctcg tattccagga ttcccgcctg ctccagcaga tccatatcca cggtaatgct
     3361 cccaacgtag tctaattccg cttgtgttac tgttgcgcgg tgtatttttg ctttcaacat
     3421 tttaattgtc atcgcctagt tctccaattc atacataaaa ttatcgatca gtcttgtctt
     3481 tccgatatag accgctatcg cggcaaggat tcctctgtcg atcttctcca cctgctgcat
     3541 cgtgtcaaga tctacgattt ttacataatc aatctttgca agaggctctt tttcgatctc
     3601 cgctgtcatt gcggcaagaa tctttttgca gtcgcgctct cccttttcca ccatctcttt
     3661 tccgaggaaa atggaacggc ttaacacgag cgctgcctcc cgctcctcta tagacagata
     3721 ggtgttgcgg gaactttttg caagtccgtc cgcttcacgg atgatcggac agccggtaat
     3781 cgtaagcggc atgttcagat ctttcaccat tctcttgatg accgcgagct gctgcgcgtc
     3841 tttctgtccg aaatatgcgc ggtccggcgt tacaatattg aaaagctttg tcacaaccgt
     3901 gcacacgccg cggaagtgta ccggtctgct gagtccgcaa agctccttgg tcagcacatc
     3961 catatccaca taagaataaa actgatcccc gtacatctcc tccggtgtcg gatggaaaat
     4021 gagatccaca ccgtgctcct cacagagctt cgcatcatgt tccagatctc tcggatagct
     4081 tgcgagatcc tccgtcggcc caaactgcat cggattgaca aaaatgctca caacaacctt
     4141 gtcattctct ccggctgcat cgatcaggct catatgtccc tcatgcaggt agcccatggt
     4201 cggcacaaac ccaatgctct gtccttcctt tttccactcc ttcacctgtg ctcttacttc
     4261 ttctatggtt gatacgattt tcataatctg ctcctttcgg cattttctat atttagtata
     4321 atttttcaat caccgagtca tcgatcttaa agcaatgttc ctcacccggg aatgttccct
     4381 cttttgtctc tttgatatat gctttgacgc cctcgcgcat cacctcgccg acattcgcga
     4441 ataccttcac aaatttcgga cggaaatcgc cgtacatggc gagcagatcc tggtatacaa
     4501 gtacctgtcc gtcacatcct gctcctgcgc cgataccgat cgtcggaatt gtaagttctt
     4561 cggagatctt agctgccagc ttcgccggca cacactccat cacgaccatg catgcacctg
     4621 ccgcttccac tgccttcgca tcagcgagaa gcttcttcgc tgcctccaca tctttgccct
     4681 gcaccttgaa tccgccgaat gcgttgacgg actgcggcgt gagtccgatg tgcgcgacaa
     4741 ccggaatgga tgacttcacg atcgcttcaa tgtgaggtgc aaattccaca ccgccctcca
     4801 gttttactgc attcgcacga ccttccttca tcagtcttcc ggcatttaca actgcatcat
     4861 acacggatgt ctgataggac ataaacggca tatccgcaac tacaaatgca tcttttgcgc
     4921 cgcgggagac cgccgccgtg tgatgaatca tatcttccat ggtcacagag agcgtatcct
     4981 catatccgag cattaccatt cccaatgaat ccccgacgag aatcgtgttg acaccggctt
     5041 catccatgat ctttgccgtc gtgtaatcat aagccgtcac catgacgatc ttatcgtgtt
     5101 cttccttctg cttctgtaac gtcaatactg tgtttttcat gtttccctcc acttttcctg
     5161 cgccccaggc gcatagactg tgttttcctg tttacactgt ttccgcgcat tccgcgccca
     5221 ggctgtgttt cccgtctctg cacaattttc tcatctccgt gtaatcacga tccggatgct
     5281 tgctctgtgc gagatctgtg agatattcag cacaggaagt atatattttc cgctgttttt
     5341 tatttaacgc ggaaatatgt ttttctacgg tagcacagtc atttctctcc agcggacctg
     5401 tgagagactg cacgcatccc tgtgtcagaa tgtgatccag atttcccttt gcaagtgcat
     5461 agagctcgct tcgcgcttcc tcctgtgcga atccgcagtc gagaagcagc tgctcggaca
     5521 tggaaaataa ggcagtcata tagttggatg agattgctgc cgccgcgtga tatttgattt
     5581 tgtcttcttt ggaaatgata cgggtgcgat tgcccatgga atagaaaaat gtctccagat
     5641 ccttcacata ggaggagtcc ccctcgagtg tgaacaatac gccggataat tctctccatg
     5701 cattttcttt gctgctgatt gcagcaagag gatgtacgga acatgcatga cagttcatgc
     5761 tgtcccagtc tgaaaagatg tctgaactgt aaagaccgct gcaatgaccg atcactttac
     5821 cggatacata gggctttgcc tgctgccaca cttctgcgat ggcggaatcc ggtacagtaa
     5881 atagaatcat gccgcaactg ctgatgattt cttctaaact ttcataacag gttgtctgtg
     5941 taaatgctgc tgcccatgca gcagacgcgc gggtcaggct gtaataacct cctacgttcc
     6001 tgcccttttc tttaaaatat ttgccaagtg acacgccgac cttgccggca ccgataaaac
     6061 cgatctgcat acgttcggtc tgtacatcat tcaatgaaat acaaacgcct cctcccggaa
     6121 gcagtatgcg gcacctttcc tgtgaaatca tggtctcctc gatccacgtc ccgtcggaaa
     6181 ctggcggtct aaggttcgtt ctcttttctt ctacggtatc atttccgcgc tgcggaatat
     6241 catccgtgat ttactataac acctggtatt tcaaatgtaa ataaaggaat aagaaaaacc
     6301 cgcggtcttt taaccgcggg ttccgataca tcttcgattt tagttgtttc ccttataaga
     6361 caggaatttt acaactgcaa tgatcgggat cagaccggat acacagccga ttgcctgtgt
     6421 ctcgctctcc tgctttccct
//
LOCUS       DBMDQJ010000034         6382 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_4963_length_6382_cov_1.443560, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000034 DBMDQJ010000000
VERSION     DBMDQJ010000034.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4963_length_6382_cov_1.443560"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(23..625)
                     /gene="cas1"
                     /locus_tag="ACZO9R_01270"
     CDS             complement(23..625)
                     /gene="cas1"
                     /locus_tag="ACZO9R_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR0911736.1"
                     /translation="MKKLLNTLYVTTPDVYLVLDGENVVVKKEDRVAMRIPIHNVEAI
                     VAFNYVGASPALMRKCNENNVNLSFFHNDSFCARMIGQENGNVVLRKIQYRLSEREDE
                     SLTIARNMILGKVHNERYVLERAIRDHALRLDTEKIKDASEQLKQYLRAVQDSGSAEE
                     LRGYEGKAASTYFSVFDELILQNKESFVFHGRNKLSPPDS"
     gene            complement(622..1287)
                     /gene="cas4"
                     /locus_tag="ACZO9R_01275"
     CDS             complement(622..1287)
                     /gene="cas4"
                     /locus_tag="ACZO9R_01275"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR0911737.1"
                     /translation="MEYQEEDYLLLSGIQHFVFCRRQWALIHIEQQWEENVRTFEGRV
                     MHENAHNADFHEKRNGVIMVRAMKVFSREMGISGECDVVEFHPAPEGITLAGQKGTYI
                     AVPVEYKRGKPKPHNADEMQLMAQAMCLEEMLMTRIPKGYLYYGETRRRTEVLFTEEL
                     RQSVRSCFEEMHAYYSRKYTPMVKTGTYCRQCSLNNICLPELSGAQPADAYVSEMLKE
                     ELQ"
     gene            complement(1317..2204)
                     /gene="cas7c"
                     /locus_tag="ACZO9R_01280"
     CDS             complement(1317..2204)
                     /gene="cas7c"
                     /locus_tag="ACZO9R_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HIR0911738.1"
                     /translation="MGEAIRNRYDFVVLFDVENGNPNGDPDAGNMPRIDPETGYGLVT
                     DVCLKRKIRNYVETLKEDTTGYKIYIKDNVPLNRSDLGAFQSLGIDEKEIKNLKKTDP
                     MLDVKVRDFMCQNFYDIRTFGAVMTTFVKANLNCGQVRGPVQMGFAKSVDPILPQEIT
                     ITRVAITTEEDAAKKGTEMGRKYIVPYGLYRVEGYVSANLARKVTGFSEEDLELLWKA
                     ILNMFENDHAAARGKMAVRKLIIFKHDSELGNAPSYKLFEAVKVARKPGVDLARAFSD
                     YEVTLPEQLPEGVTCTCME"
     gene            complement(2206..3957)
                     /gene="cas8c"
                     /locus_tag="ACZO9R_01285"
     CDS             complement(2206..3957)
                     /gene="cas8c"
                     /locus_tag="ACZO9R_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002595865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HIR0911739.1"
                     /translation="MILQALVEHYEDLLKQGRIAKLGWGKVKVSYGLNLDRQGHVLGL
                     IPLKVAQETGGSKKKTVFVARMMEVPHPEKRSVAVCPNFLCDNATYILGVDEKGKPER
                     TSQCYEACMEKHMELLSDIHTPAAEAICHYFEYWSPDAARQCTDIEDNWSDLMAGVNL
                     LFYYEGEAVTEDPAIREAWQQFYDRKDESGQTLCLVTGKKDKLAVLHPSIKGVRGAQA
                     MGASLVSFNAASFCSYEKEQGANAPVGEYAAFAYTTALNELLNDRAHTGTIGDTTVVC
                     WAEGGESLYQDVGMMAMFGLTDESGMTDGELRAIIGKVANGEPVQIEDIRLDPDRHFY
                     VLGLAPNAARLTVRFFMRDSFGTMIRHVNEHQERLRIVNARQKEYPIIPIWKLMSETV
                     NQNARDKSPSPQMAADTLKAVLTGGKYPASLLNAVMLRIRAEHRITRERAAMIKAYYL
                     KNENKDCPKEVLQEMINEESKNVPYTLGRLFAVLEHLQQEANPGINTTIKDKYFNSAA
                     ATPAQIFPLLLNLAQKHLRKLANEGHKVYFNRQIGKLAEVIGESFPKRLNLPEQGAFQ
                     LGYYCQTQKRYTKKEEN"
     gene            complement(3954..4589)
                     /gene="cas5c"
                     /locus_tag="ACZO9R_01290"
     CDS             complement(3954..4589)
                     /gene="cas5c"
                     /locus_tag="ACZO9R_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas5c"
                     /protein_id="HIR0911740.1"
                     /translation="MEVWGNFACFSRPEMKVERVSYDVMTPSAARGMLEAVYWHPGLQ
                     WHIDNIYVLAPIQMTNIRRNEVQSKVLGNSVRAAMTGGRTELYINTSKDIVQRASLVL
                     QDVRYVIEAHFTLTEKAAAGDNPGKFQDIIKRRLRKGQCYHEPYFGCREFPANFKEWE
                     GGEIPAIQETRDLGYMLWDLDFSDPANIRPLFFRAKMENGVIHVADCEVLR"
     gene            complement(4806..6311)
                     /gene="cas3"
                     /locus_tag="ACZO9R_01295"
     CDS             complement(4806..6311)
                     /gene="cas3"
                     /locus_tag="ACZO9R_01295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HIR0911741.1"
                     /translation="MSKGSELPPGIYTLTVPTGGGKTTASLGFALRHAIQSKMKRIIY
                     VIPYTSIIDQNAEVFRSILGEKNVLEHHSGILYDLTEDKAENEAAYRKALATENWDMP
                     VIVTTVVQFFESLYANRSSKCRKLHNMANSVIIFDEAQTMPIPYLEPCVAAIAELAAH
                     YRSTIVLCTATQPVLDEMIQKYMPQTQVREICEHTDILFQKLRRTSIVAAGNMEREKL
                     IDCLTAQEQVLSIVNSRNLAQELFEEIPREGAFCLTTLLHPEHRKEKLREIKERLEHH
                     VPCRVIATSLVEAGVDLDFPCVYRQENGLDALIQAAGRCNREGTHSPEESKVYLFGLK
                     GDASVYFAQNISALRQTLRCYEDPTAPEAIAFYFKQYREILGRENLDQKKIMDAFTAG
                     KGINGDCFPFATVAREFHLIESETQTVYIPVGEAELWIEEMEKGKVSRKLMRKLGQYA
                     VNVYPNHLKALYDAGCVEGIGEGMYVLRDMNQYHEDTGLQMDVSTGYGLFG"
BASE COUNT         1496 a         1814 c         1163 g         1909 t
ORIGIN      
        1 agaatgaatg taaacggtat gcctatgaat ccggcggcga gagcttattc cttccgtgaa
       61 atacaaaact ttctttgttc tgaagaatca gttcatcaaa tacactaaaa tacgtacttg
      121 ccgcttttcc ttcatatccc cgcagttcct ccgcactgcc actgtcctgc accgctctta
      181 gatactgttt taactgctcc gacgcatctt ttatcttttc tgtatcaagg cgcaatgcat
      241 gatcccggat tgcccgctcg agtacataac gctcattatg tacctttccg agaatcatat
      301 ttcttgcaat cgtaagactt tcatcttccc gctccgacag ccgatattgt attttccgca
      361 aaacgacatt accgttttcc tgtccgatca ttctggcaca aaacgaatca ttgtgaaaaa
      421 aactcagatt cacattgttc tcattgcatt tgcgcatgag cgccggactg gcacctacat
      481 aattaaacgc aacgattgct tctacattgt gaatcggtat ccgcattgcc acccgatcct
      541 cttttttgac gacaacgttt tctccatcca atacaagata tacatccggt gttgttacat
      601 aaagagtatt cagcaatttc ttcattgcaa ttcctccttt aacatctccg acacataggc
      661 atccgcaggc tgtgctccgg aaagttccgg cagacagatg ttgtttaagg aacactgcct
      721 gcaatatgtg cctgtcttta ccatcggtgt atattttctg ctgtaatatg catgcatttc
      781 ttcaaaacaa gaccgtacac tctgtctcaa ttcctctgta aacagcactt ccgttctgcg
      841 tctcgtttct ccataataca aatagccctt tggaattctg gtcataagca tctcttccag
      901 acacattgcc tgcgccataa gctgcatttc atctgcattg tgcggctttg gttttccgcg
      961 cttatattcc accggcacgg caatgtacgt tcccttttgt ccagctaatg tgatgccctc
     1021 cggtgccgga tgaaactcca caacatcaca ttctccgctg attcccatct ctctggagaa
     1081 aaccttcatc gcccgcacca tgataacccc gtttcttttt tcgtgaaagt ccgcattgtg
     1141 cgcattttca tgcatgaccc ttccctcaaa tgtccgtaca ttctcttccc actgctgctc
     1201 aatatgaatc agagcccatt gtctgcggca aaaaacgaaa tgctgaattc ccgataataa
     1261 taaataatcc tcctcctgat attccatatg tttctccctg cgatagccac gcaatactat
     1321 tccatacagg tacaggtaac tccctcaggc agctgctccg gcaatgtaac ctcataatct
     1381 gaaaacgccc ttgccaaatc aactccaggt tttcttgcca ccttgacagc ttcaaacaat
     1441 ttataagacg gtgcatttcc aagttcgctg tcgtgcttaa aaatgatcag ttttctgact
     1501 gccatcttgc cgcgtgccgc cgcatgatca ttctcaaaca tattcagaat tgctttccag
     1561 agcaattcca gatcctcttc cgaaaaaccg gtcacttttc tcgccagatt tgcagacaca
     1621 tatccctcta cccggtacag accatacgga acaatatatt ttcttcccat ttccgttcct
     1681 tttttggcag catcctcctc cgtcgtgatt gccactctgg taatcgtgat ctcctgtgga
     1741 agaatcggat caacggattt cgcaaatccc atctgcaccg gtccgcgtac ctgtccacag
     1801 tttaaatttg ctttcacaaa agtggtcata actgctccga atgtacgaat atcatagaaa
     1861 ttctgacaca taaagtcacg aacctttacg tccagcattg gatctgtctt ctttaaattc
     1921 ttaatctcct tttcgtcaat gccaagtgac tggaatgctc caagatcact gcggtttaac
     1981 ggcacattgt ccttgatata gattttatat ccggtcgtat cctccttgag tgtctccaca
     2041 tagttgcgga tctttcgctt caggcacaca tctgtcacga gaccgtaacc cgtttccgga
     2101 tcaattctcg gcatattgcc ggcatccgga tctccatttg gatttccatt ctccacgtca
     2161 aatagtacta caaaatcata tctgttccta attgcctctc ccatcttagt tttcctcctt
     2221 ctttgtatat cgtttctgtg tctggcaata ataccccagc tgaaaggctc cctgttccgg
     2281 cagatttaaa cgctttggga aactctcccc aatcacttcc gcaagtttcc caatctgtcg
     2341 gttaaaataa actttgtgtc cttcgtttgc cagtttccgc agatgcttct gcgccagatt
     2401 cagcaatagc ggaaaaatct gtgccggggt cgctgctgcc gaattgaagt acttgtcttt
     2461 gatcgtcgtg ttaattcctg gattcgcttc ctgctgcaaa tgctctaaaa ccgcaaacag
     2521 gcgccccagt gtgtatggta catttttgct ctcctcattt atcatttcct gcaatacctc
     2581 cttgggacag tctttgttct cattcttcag ataataggcc tttatcatag ccgctcgctc
     2641 tcgtgttatc ctgtgttccg cccggattct taacataacg gcgtttagca gcgatgccgg
     2701 atactttccg cctgtcagaa ccgcttttag cgtatccgct gccatctgtg gtgacggaga
     2761 cttgtctctc gcattttggt ttacggtctc tgacatcaat ttccagattg gaatgatggg
     2821 atactccttt tgccgcgcat taacaatccg cagacgctcc tgatgttcat tgacatgccg
     2881 gatcatagtg ccaaaagaat ccctcataaa aaatcggaca gtcagtctgg ccgcattcgg
     2941 tgcaagtccc agcacataaa aatgtctgtc gggatccagt cgtatatcct cgatctgtac
     3001 cggctccccg tttgccacct tcccgataat tgcccgaagt tcgccatctg tcataccact
     3061 ctcatctgtc agaccgaaca tcgccatcat accgacatcc tgatagaggc tctctcctcc
     3121 ttccgcccag cataccacgg ttgtatcccc gatagttccc gtgtgagccc ggtcattcag
     3181 aagttcattt aacgctgtgg tgtaggcaaa tgccgcatac tcacccaccg gagcattggc
     3241 accctgttcc ttctcatagg aacagaagga cgctgcatta aaagatacca gtgacgctcc
     3301 catcgcctgt gcgccgcgaa cccccttaat gctcggatgc agcactgcaa gcttgtcttt
     3361 tttcccggta accagacaca acgtctgccc gctttcatcc ttcctgtcat aaaactgctg
     3421 ccatgcttca cgtattgcag gatcctctgt cactgcttct ccttcataat aaaatagaag
     3481 atttactcct gccatcagat cgctccagtt atcctctatg tcagtacact ggcgcgccgc
     3541 atccgggctc caatactcga aataatgaca gatcgcttct gctgccggcg tgtggatatc
     3601 agaaagcaat tccatgtgct tttccatgca ggcttcatag cactgactgg ttcgctctgg
     3661 ttttcctttt tcatcaacac caagaatata ggtcgcattg tcgcacaaaa agttcgggca
     3721 aaccgccacg gatctcttct ccggatgagg tacttccatc atccgcgcaa caaaaaccgt
     3781 ttttttcttg ctgcctcccg tctcctgcgc caccttcaac gggatcaacc ctagtacgtg
     3841 tccctgccga tccagattca gtccatagga aacttttact tttccccaac ccagctttgc
     3901 aatccgcccc tgcttcaaca aatcttcata atgttccacc aatgcctgta aaatcatcgc
     3961 agcacctcgc aatctgccac atggatcacg ccgttttcca tctttgcacg aaagaacagc
     4021 ggacgaatgt ttgccggatc tgagaagtca agatcccaca acatatagcc gagatccctt
     4081 gtctcctgaa tagccggaat ctctccgccc tcccactcct tgaaattcgc cgggaattcc
     4141 cgacatccaa aatatggttc gtgatagcac tgtccctttc tcaggcggcg ctttatgata
     4201 tcctgaaact ttcccggatt atctccggct gccgccttct ctgtaagggt aaaatgtgct
     4261 tcaatcacat atctgacatc ctgcagaacc aatgatgctc tctgcacaat atcctttgat
     4321 gtgttaatat acagttctgt gcgtccgccg gtcatagctg ctcttacact gttccccagc
     4381 accttggact gcacctcatt tctccgtatg tttgtcatct gaataggcgc caacacataa
     4441 atgttgtcaa tatgccactg caggcctggg tgccagtata ctgcctcgag cattccccgc
     4501 gccgccgacg gtgtcatcac atcatagctg acgcgttcca ccttcatctc cggccggctg
     4561 aagcatgcaa aattccccca cacttccatt tttactgcca catatcattc ctcctttcat
     4621 ctgtttttca tttgttatat ttttaaaata catcatctta ctatccatgt caatacattt
     4681 tttattgaaa aatgcaatat atttgtatac actggtattg tcacttttat aagtgtggat
     4741 tgaaatattt gatatatttt tctttcatca gggcggcaga tttctgccgc cttttgacca
     4801 ctcttttatc cgaacaatcc atatcctgta ctcacatcca tctgaagtcc ggtatcctca
     4861 tgatactggt tcatatcccg aagcacatac atcccctctc cgattccttc cacacagccg
     4921 gcatcgtaca atgcttttaa atgattcgga tatacattca ccgcatactg ccccagcttt
     4981 cgcataagtt ttcttgagac ctttcccttt tccatctcct ctatccacaa ttccgcttca
     5041 ccgaccggaa tataaaccgt ctgtgtctca ctctcaatca gatgaaattc ccttgccacc
     5101 gttgcaaacg gaaagcaatc cccattgatt ccttttccgg cagtaaatgc atccattatc
     5161 ttcttttgat ccagattctc tctccccaga atctcacgat actgcttaaa atagaatgcg
     5221 atcgcctccg gcgctgtcgg atcttcgtaa caacgcaggg tctgccttaa cgcgctgatg
     5281 ttctgtgcaa aatataccga tgcatcgcct ttcaaaccga acagatatac cttgctctcc
     5341 tctggggagt gcgtcccctc ccggttgcat ctgcccgccg cctgtatgag tgcatccagt
     5401 ccgttctcct gacgatatac acacggaaaa tccagatcta cgcccgcctc cacaagtgaa
     5461 gtagcgatca ccctgcaggg cacatgatgt tccagtctct ctttgatctc gcgcaatttc
     5521 tctttgcgat gctccgggtg caatagtgtt gtgaggcaaa atgctccctc tctcggaatt
     5581 tcttcaaaaa gttcctgtgc cagatttcga ctgttgacaa tggaaagcac ctgctcctgc
     5641 gcagtcagac agtcgatcaa tttttcccgt tccatattgc cggctgcaac aatcgatgtt
     5701 ctgcgcagtt tttgaaaaag aatatctgta tgttcacaga tttctcttac ctgcgtctgc
     5761 ggcatatatt tttggatcat ttcatccaat accggctgcg tagccgtaca aagaacgatt
     5821 gttgaacggt aatgcgccgc cagttctgcg attgccgcaa cacacggttc cagatatgga
     5881 atcggcattg tctgtgcctc gtcaaaaata atgacactgt ttgccatgtt atgaagcttt
     5941 ctgcattttg aactgcggtt cgcatacaac gattcaaaaa actgcaccac agtcgtcacg
     6001 atcaccggca tgtcccagtt ttcggtggcc agagccttgc ggtatgccgc ttcattctcc
     6061 gccttatcct ccgtcagatc gtagagaatt ccggaatgat gttccagaac atttttctct
     6121 cctaaaatag agcggaacac ctctgcattc tggtctataa tcgatgtgta cggtatcaca
     6181 taaataatcc gcttcatctt tgattgtatc gcatgccgca gtgcaaagcc caaagatgcc
     6241 gtggttttcc cgcctcccgt gggtaccgtc agcgtataga ttccgggcgg taattcactt
     6301 cctttgctca tacaatcctg caaaacctga tttcttatac tgcagagtat tttctgtttg
     6361 tcgcttttga aatccgtttt tt
//
LOCUS       DBMDQJ010000035         6290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5043_length_6290_cov_1.858135, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000035 DBMDQJ010000000
VERSION     DBMDQJ010000035.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5043_length_6290_cov_1.858135"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(13..1752)
                     /locus_tag="ACZO9R_01300"
     CDS             complement(13..1752)
                     /locus_tag="ACZO9R_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR0911742.1"
                     /translation="MGYVRLKQLIEQNAWREAGRELAQYMNGEWDDELAVLAATVFSA
                     LGDWEGAYTCIAQGLQYNYRNYELYLLLGNYYERKNCNQAWLCYENALYYCSDEDDRR
                     IIQQHKERVQQDDRWCVHKVSIVILTYNLKDMNMQCIGSIRDTCAPSSYELVVVDNAS
                     TDGTLEWLQEQKDMTLVSNQENKGFPAGCNQGIKAAEPENDILLLNNDTIVLPNSIFW
                     LRMGLYEEERIGTTGSMSNSVINGQRIEAKLAGVEEYITYGTAMNIPMENALEKKTWL
                     VGFAMLLKRKALDEVGLLDERFSPGQNEDVDLCIRLNLAGWQMRLCHNSFIVHYGHGN
                     GRNSYIWKQTFSVTSEKFREKWGFDMSYYTYSRKELIAMISEPKESRIRVLEVGCGCG
                     ATLAHIAYEWPEAKVSGIEIQDDIAKIGANYLDIRQGNIETMQIPYEKDTFDYIILAD
                     VLEHLHDPERILQKLLSYLKADGSILCSVPNILNRHVISDLLRGKLEYQDAGILDRTH
                     LRFFTMDSIVRVFERCGMEVTQMMATYDTEELDAEAEELMNALYQIPHIADRQLFQVY
                     QYVFRAKREREAE"
     gene            complement(1809..2930)
                     /gene="pseC"
                     /locus_tag="ACZO9R_01305"
     CDS             complement(1809..2930)
                     /gene="pseC"
                     /locus_tag="ACZO9R_01305"
                     /EC_number="2.6.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4,
                     6-dideoxy-N-acetyl-beta-L-altrosamine transaminase"
                     /protein_id="HIR0911743.1"
                     /translation="MIPYGRQTIEEDDIEEVVKVLKSDFLTTGPKVAEFERAVAEYVG
                     AKYAVAISNDTAALHAACHAAGIGPGDEVITTPITFAASANCVLYCGGTPVFADIDPQ
                     TYNIDPEDIRCKITDKTKAIIPVHLAGQPCDMDAIHAIAKEHHLIVIEDAAHALGSEY
                     KGQRIGSMSDMTTFSFHPVKPITTGEGGMIVTDSEELYRKLVLFRSHGITRDPDLMTR
                     NEGSWFYQQLDLGYNYRMTDIQCALGCSQMRKLDRFLARRREITKRYDEAFADCANIV
                     TPYQLPETNSGWHLYIIQVKNHDRKTVFEQLRENGIAVNVHYIPVYYHPYYQEHGYRE
                     VHCPVAEEVYSHIISLPVYPGLTDEQQDTVIETVKRLTK"
     gene            complement(2930..4177)
                     /locus_tag="ACZO9R_01310"
     CDS             complement(2930..4177)
                     /locus_tag="ACZO9R_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0911744.1"
                     /translation="MPVEDRKDTIAFIICVNNELYFEECKYYIEHLEVPAGYDIDVIG
                     IWEADSMCAAYNLGMRSSDAKYKVYMHQDVFIRDSRFLEKTLRIFKEHPKTGMIGMIG
                     GIGIPENGVVYTAWNVGKVDCREPDLSYVLLCGPNQTKDQIVDAVDGLLMMTQYDLPW
                     REDLFSDFDFYDVSQAFEFRRAGYEIVVPYQKEPWVVHDCGFAKLTNYDRNRKICMEE
                     YPEFLNAPEEMELISNDEWNCLSSQLAKEIRKMLDRGKWSEAERVLQIYHQKQMKSSE
                     LEMLGIICEIEQAERKAGVTHFLFPQEGGYQEIYKTYVTVRFLLHRLEFGMPSENYQE
                     LIHAVESGNISVEALIIMILHGVLNKRYVVETLESWKLPDVKSAEQLKIFYEKVLQRN
                     CDVMVSYSKRAKMELEKYSKEEQ"
     gene            complement(4189..5223)
                     /gene="pseG"
                     /locus_tag="ACZO9R_01315"
     CDS             complement(4189..5223)
                     /gene="pseG"
                     /locus_tag="ACZO9R_01315"
                     /EC_number="3.6.1.57"
                     /inference="COORDINATES: protein motif:HMM:TIGR03590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,4-diacetamido-2,4,
                     6-trideoxy-beta-L-altropyranose hydrolase"
                     /protein_id="HIR0911745.1"
                     /translation="MIVFRVDANEQVATGHLMRTLSIALACRKCGLMCEFWLAEEKET
                     ERIAKAGFPYRILGTRWDALEDELPVMKQRLQAQQVDWLVVDSYCVTVRYLAELNRIV
                     PVFYVDDFRKEHYEASALLQYIPCRERQKTPEGLHEKDGVQYLTGLLYAPLREEFSHL
                     SFDKPREKSILLSTGGTDPYNMAGRILAYGTQMQELQDYLWHVIVGSMNRNEDMLREL
                     AAENPKIILHKNIPDMWRYMQQCEAAVSAGGTTLLELCACGIPTVCFAFADNQLEFAE
                     EMAEHNLLIYAGDVREKDDAPHVICENLVCYTKDAGKRAQSFERMRRLVDGKGAERIA
                     AFLQQYKRDV"
     gene            complement(5228..6256)
                     /gene="pseI"
                     /locus_tag="ACZO9R_01320"
     CDS             complement(5228..6256)
                     /gene="pseI"
                     /locus_tag="ACZO9R_01320"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HIR0911746.1"
                     /translation="MKLYEKMNSGVYVIAEMSANHAGKIENAFKIIEEAAKAGADCVK
                     IQTYTADTLTIDCREEPYQIKGGLWDGYNYYQLYQAAYTPWEWQRALRDKCREAGVDF
                     LSTPFDRTAVDFLEDLDVEFYKIASFELVDIPLIEYTAGKGKPMIMSCGMASEEEIKE
                     ALEACRRQGNEQVVLLKCCSQYPAQYEDMNVATIADMKERFGVPVGLSDHSMGSLADV
                     VAVSLGAQVIEKHVCLDRTIDNPDAAFSMEIGEFAQMVQDVRNACVIKGKATYELTEH
                     EKSSLKYRRSLVAVKPIAEGEAFTEENVRSIRPAIGIKPKYYRTLLGRKAKKAYRFGE
                     PILPGEVE"
BASE COUNT         1391 a         1956 c         1243 g         1700 t
ORIGIN      
        1 tttgtacttt tttcattccg cctcccgctc cctctttgct cggaaaacat actggtacac
       61 ctgaaacaac tgtctgtccg caatatgcgg aatctgatac agtgcgttca tcaattcttc
      121 cgcttccgca tccagctctt ccgtatcata ggtcgccatc atctgtgtga cttccattcc
      181 gcaccgttca aataccctca caatactgtc cattgtgaaa aagcgcagat gcgtgcggtc
      241 taaaattcct gcgtcctggt actccagctt tccgcgcaga aggtcgctga ttacatggcg
      301 gtttaaaata ttgggaacac tgcacagaat actgccgtcc gccttcagat aggacagtaa
      361 tttctgcaga atccgctccg gatcatgcag gtgctccaac acatccgcca gtatgatata
      421 gtcaaacgta tctttttcat acgggatctg catggtctca atgtttccct gccggatatc
      481 cagataattt gcaccgattt tcgcaatgtc atcctggatc tcaattccgc tcaccttcgc
      541 ttccggccac tcatacgcaa tatgcgcaag cgttgcaccg catccgcatc ccacttccag
      601 cacgcgtatt ctgctctctt tcggctcgga aatcatcgca atcagttctt ttctgctgta
      661 ggtataatag gacatatcga agccccattt ttcccggaac ttctcacttg taactgagaa
      721 cgtctgcttc cagatatacg agttcctgcc gtttccatga ccgtaatgca cgataaaact
      781 attgtggcag aggcgcatct gccaccccgc cagattcagc cggatgcaca gatctacatc
      841 ctcgttctgc cccggggaaa accgttcatc cagaagtccg acctcatcca gcgccttccg
      901 tttcagcagc atcgcaaatc ctaccagcca ggtctttttc tccagcgcat tttccatggg
      961 gatattcatt gccgtgccgt atgttatgta ttcctccaca ccggcaagct tcgcctcaat
     1021 tctctgcccg ttgatcacgc tgtttgacat gcttcccgtg gtgccgatcc gttcctcctc
     1081 atataacccc atcctgagcc agaaaataga attgggcaga acaatcgtat cattgttcag
     1141 aagaaggata tcattttccg gctctgccgc cttgattccc tgattgcacc cagccggaaa
     1201 gcccttgttt tcctggttgc tcacgagtgt catatctttt tgctcctgaa gccattccag
     1261 cgtgccgtct gtagatgcat tgtccaccac gaccaactca tagctcgacg gagcgcaggt
     1321 gtcccggatg ctgccgatac actgcatatt catgtctttg aggttatatg tcaatatcac
     1381 aattgaaacc ttatgcacgc accaccggtc atcctgctgt acacgctctt tgtgctgctg
     1441 tatgatcctg cgatcatctt catcgctgca ataatagagc gcattttcat agcagagcca
     1501 cgcctgattg caatttttgc gctcataata attgcccagc agcagataca gttcataatt
     1561 tctgtaatta tactgcagcc cctgcgcgat acaggtatag gcgccttccc agtcgcccag
     1621 cgcagagaaa accgttgctg ccagcacggc gagttcatca tcccattccc cgttcatata
     1681 ctgcgcgagt tctcttccgg cttccctcca cgcattctgc tcaataagtt gctttaatct
     1741 tacatatccc atcccgctct attctcctgc tgtttctgaa gatctgcctc tccctcatcg
     1801 tccggaaact attttgtcag ccgctttacc gtttcaatga ccgtatcctg ctgctcgtcg
     1861 gtaagccccg gataaaccgg aaggctgatg atgtgcgagt agacttcctc cgccaccgga
     1921 caatgtactt ctctgtaacc gtgttcctgg tagtacggat gataatacac cggaatatag
     1981 tggacattca ctgcgatgcc gttctcccga agctgctcaa acacggtctt tctgtcgtgg
     2041 ttcttcacct gtatgatata gagatgccat ccgctgttcg tctccggcag ctgatacggc
     2101 gtcacaatat tcgcgcagtc cgcaaacgcc tcatcgtagc gctttgtgat ctcccttctg
     2161 cgagcaagga aacggtccag cttccgcatc tggctgcagc caagcgcaca ctggatatct
     2221 gtcatgcggt agttatagcc gagatcgagc tgctgataaa accacgatcc ctcatttctc
     2281 gtcatcagat ctggatctct ggtaattccg tggctgcgga acagcactaa tttccggtac
     2341 agttcctcac tgtccgtcac aatcataccg ccttcgcctg tcgtgatcgg ctttaccgga
     2401 tgaaagctga atgtcgtcat atccgacatg gagccgatgc gctgtccctt atattccgag
     2461 ccgagcgcat gcgccgcatc ctcgatcacg atcagatgat gctcctttgc gatcgcatga
     2521 atggcatcca tatcgcaggg ctgtcctgcc agatgcaccg gaataatcgc tttggtctta
     2581 tccgtgatct tgcatcggat atcctccgga tcaatgttgt acgtctgcgg atcaatgtct
     2641 gcaaacaccg gcgttccgcc gcagtacagc acacagttgg cggatgccgc aaacgtgatc
     2701 ggcgtggtga ttacctcatc ccccggtccg attcccgccg catggcatgc cgcatgcagt
     2761 gccgcagtat cgtttgaaat agcgaccgca tatttcgcgc ccacatactc tgccactgcc
     2821 cgctcgaact ctgccacctt cggtcctgtc gtcaagaaat ctgatttcag cacctttact
     2881 acttcctcaa tatcatcttc ctcaatcgtc tgtcttccgt atggtatcat cactgttcct
     2941 ccttgctgta cttttccagc tccatctttg ccctcttact gtaggaaacc ataacatcac
     3001 aatttctctg taaaaccttt tcataaaata tctttaattg ttctgcactc ttcacatccg
     3061 gcagcttcca gctttccaac gtctcaacaa catagcgctt atttaatacg ccatgcagga
     3121 tcattatgat aagtgcctct actgatatgt taccgctttc taccgcatga atcaattcct
     3181 gataattctc tgacggcatc ccaaactcca gacgatgtaa cagaaaccgt accgttacat
     3241 atgtcttata tatttcctga tagccgccct cctgcggaaa cagaaaatgc gttactcccg
     3301 ccttgcgttc cgcctgttca atctcacaga taattccaag catctccagt tccgaacttt
     3361 tcatctgctt ctgatgatag atttgaagca cgcgctccgc ttccgaccat tttccccggt
     3421 caagcatttt ccgaatctct tttgcaagct ggctgctcag acaattccat tcatcatttg
     3481 aaataagttc catttcttcc ggtgcattta aaaattccgg atattcttcc atacagattt
     3541 tccggttcct gtcataattg gtgagctttg cgaatccgca gtcgtgcacc acccagggtt
     3601 ctttctgata aggcaccaca atctcatatc ctgccctgcg gaattcaaac gcctgcgaca
     3661 catcatagaa atcaaaatcc gaaaacagat cctctctcca cggcagatca tactgtgtca
     3721 tcatcaacag tccgtccacc gcatcaacga tctgatcctt ggtctggttc ggtccgcaca
     3781 gcaggacata ggaaagatcc ggctccctgc agtctacttt tcccacattc caggcagtat
     3841 aaacaacgcc gttctccgga ataccgattc ctccaatcat cccgatcatc cccgtcttcg
     3901 ggtgctcctt aaaaatacgc agcgtcttct ccaggaaacg gctgtcgcgg ataaaaacat
     3961 cctgatgcat atagacttta tattttgcat cgctgcttcg catccccaga ttgtacgccg
     4021 cgcacatgga atccgcctcc cagatgccga tcacatctat gtcatatccc gccggaactt
     4081 ccagatgctc tatgtaatac ttgcactctt caaaatacag ctcattattc acgcatataa
     4141 tgaatgcgat tgtgtctttt ctgtcctcta ccggcatgat tttctacctc atacatctct
     4201 tttatactgc tgcaaaaatg ccgcaatccg ctctgcgccc tttccgtcca ccagccttct
     4261 catccgctca aagctctgcg ccctcttccc ggcgtctttc gtatagcaga ccagattttc
     4321 acagattaca tgcggcgcat catccttttc tctcacatcc cccgcataaa ttaacaggtt
     4381 gtgttctgcc atctcctccg caaattccag ctgattgtcc gcaaacgcaa aacagaccgt
     4441 cggaatcccg caggcgcaca gttccagaag cgttgtaccg cccgccgaga ccgccgcctc
     4501 gcactgctgc atgtagcgcc acatatcggg aatattcttg tgaagtatga tcttcgggtt
     4561 ctctgccgcc agttcacgca gcatgtcctc attccggttc atgcttccga caatcacatg
     4621 ccacagatag tcctgcaatt cctgcatctg tgtgccgtac gcaagaattc tgcccgccat
     4681 attgtaagga tcggttccgc ccgtcgagag aagaatactc ttttcccgtg gcttatcaaa
     4741 ggaaagatgt gaaaactctt cccggagcgg cgcatacaaa agtccggtaa ggtactgtac
     4801 cccgtccttt tcatgcagcc cctccggcgt cttctgtctc tccctgcacg gtatgtattg
     4861 gagcagcgcc gacgcctcgt agtgctcctt tctgaaatcg tcaacataga ataccggcac
     4921 tatccggttt aattccgcca gatagcgcac cgtcacacag taggaatcca caaccagcca
     4981 gtccacctgc tgtgcctgta accgctgctt catgaccgga agttcatcct ccagcgcatc
     5041 ccagcgcgta ccaagaatcc gatagggaaa tcccgccttt gcgatccgct ccgtctcttt
     5101 ttcctctgcc agccagaact cacacatcag cccgcacttc ctgcacgcca acgcgatcga
     5161 aagcgttcgc atcagatgtc cggttgccac ctgctcgttc gcatcaaccc gaaatacaat
     5221 catctgtcta ctccacctca cccggaagaa tcggctctcc gaagcggtac gcctttttcg
     5281 ccttgcgccc cagcaacgtg cgataatact tcggcttgat accgatggca ggccggatgc
     5341 tgcgcacatt ctcctcggtg aaagcctccc cctccgcaat cggttttacc gcaaccagcg
     5401 atctgcggta tttcaaactg ctcttctcat gttcggtcag ttcatacgtt gcctttccct
     5461 taatcacgca ggcattccgc acatcctgta ccatctgcgc aaattcgccg atctccatgg
     5521 aaaaagctgc atccggattg tcaatcgtcc gatccaggca gacatgcttt tcgatgacct
     5581 gcgctcccag tgacactgcc acaacatccg caagcgatcc catcgagtga tccgacaggc
     5641 ccaccggcac gccgaaccgc tccttcatat ccgcgatcgt tgccacattc atatcctcat
     5701 actgcgccgg atactggctg cagcatttca gaagcaccac ctgctcgttt ccctgtctcc
     5761 ggcacgcctc aagggcctct ttgatctctt cctccgacgc cattccgcag gacataatca
     5821 tcggttttcc cttgcccgcc gtgtactcga tcagcggaat atccaccagc tcaaaggacg
     5881 cgattttgta gaactccaca tccagatcct ccagaaaatc gaccgctgtc cggtcaaacg
     5941 gcgtagacaa aaaatccaca ccggcctccc tgcacttatc ccgcagcgcc cgctgccatt
     6001 cccacggggt ataggccgcc tggtagagct gataataatt atagccgtcc cacagaccgc
     6061 ctttgatctg atacggttcc tccctgcagt caatcgtgag cgtatccgcc gtgtaggtct
     6121 gtatcttcac acaatccgcg ccggctttcg ccgcctcctc aatgattttg aatgcgttct
     6181 caatttttcc cgcatggttt gcggacatct ctgcaatcac atagaccccg ctgttcattt
     6241 tctcatatag tttcatctgt gacagccgcc tctcttttct atacagactt
//
LOCUS       DBMDQJ010000036         6290 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5044_length_6290_cov_1.629019, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000036 DBMDQJ010000000
VERSION     DBMDQJ010000036.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5044_length_6290_cov_1.629019"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="ACZO9R_01325"
     CDS             <1..495
                     /locus_tag="ACZO9R_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR0911747.1"
                     /translation="REWTDCPILVVSGQTGYMDKVNALYAGADDYIVKPFHEQELAAR
                     IYTALRRRIPGGQGSFYQAGDLKIDFSRRQVSLAGEEIHFSPVEYRILECLALNSGTV
                     VTYQMLLEKIWGPYASSGSRILRVNMTNIRKKIEKIPMDPTYITTIPRVGYRMLESQT
                     ASVV"
     gene            complement(474..629)
                     /locus_tag="ACZO9R_01330"
     CDS             complement(474..629)
                     /locus_tag="ACZO9R_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911748.1"
                     /translation="MTIFEHIFSVIGILIIIGGFIYGIYDSYFEGKGTSSSKASASTS
                     AQTTDAV"
     gene            903..2978
                     /locus_tag="ACZO9R_01335"
     CDS             903..2978
                     /locus_tag="ACZO9R_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0911749.1"
                     /translation="MKKGKKQAKRRNANVTMAFGIREKIVLCFLVPVLFMIVLGIISY
                     RKAADGMSSSYRDSTQQTIEMALQNIDTSNSFISAEALKYVVDSDLGKYFVGIYDNDP
                     TSKRTLIDQVKTRILASQVGNSYISNIYIIPSGDLQMISTKNKAQKGIYQEYMDEMAV
                     DGKLGQWDDHHNALDEYLSIDSSRYIMVYQAAAQNGKAVIVIDIKAEAVTEFMQGLNL
                     GDGSIVGFVTAGGRELAVKRNAGDEQGTPVEGETIFADKDFYLQASETDGVRQVQYNG
                     TEYLFFHSKSEDTGATMCALVPLKVITGQAESIKQITIAGVLISSIIALLIGTAVTSG
                     IRKNMKRISGSLEVVAEGNLATTVSVKGRDEFRGLAAAANDMIAHNKQLVQKVSQATD
                     KLAASAGEVTNASGIIQEYSQDIANAIGEINEGMERQSTHAQECVSKTGTLSEEIQEV
                     NRVAREVETLVSEAEEMIRKGMDRIEQLGQRANETTAVTAKVEESIEDLKRESETINQ
                     FVSVITDISEQTNLLSLNASIEAARAGEAGRGFAVVAEEIRKLADNSAEAAGEIRNNV
                     ENISAQTGVSVESAKQAGEMVAQQTEAVREVIGVFQDMSDAMDKLFDGLKEILAGTER
                     ADKEREDTLEAVHNISSIIEETAASAEVVKKVAASLEQNVENLNGTAESLGDNMTGLK
                     TEIAVFKTE"
     gene            complement(3127..4146)
                     /locus_tag="ACZO9R_01340"
     CDS             complement(3127..4146)
                     /locus_tag="ACZO9R_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911750.1"
                     /translation="METYNDQLFPPAFLVNYTKEPAGRQRVLTYSLFRFLSCVSEEHP
                     DLAQFLADQVSCPSAEDSFEILEDYLSADDYFSPSIAADGFEPFYLYAAIVLTAGVPF
                     GTTTPVINSLIEDHAPEAASLPYADGNLPLDALLVDCRNFYAALCLCLMHHTDALAVL
                     LPQFLNAYRPEYHFSCESFVLYDFMDEYFEQHDCLHHPSFVQLTDTLVAATLNYYNTD
                     FGTLLETEIAQNLSGTGSRFAGLKRYGSVRLPAITDTDKACHMLANLFRYAALYELRS
                     HLFDFHLDEDRLITLDNWQTELHWHYVQYANVYQLALDSFQSAVLSRELLHRQFLLNL
                     SCLNL"
     gene            4314..4895
                     /gene="lepB"
                     /locus_tag="ACZO9R_01345"
     CDS             4314..4895
                     /gene="lepB"
                     /locus_tag="ACZO9R_01345"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR0911751.1"
                     /translation="MPKQNQPKEEKREKGTAQAVIREILSWVIPFVIALAVAMFLKNY
                     IIINADVPTGSMENTIMPGDRLIGNRLAYRSKSPERGDIVIFRYPDNEEELYVKRVIG
                     LPGETVDIRDGYIYIDGADTPLDEDYLKEDWTVATGNYHFEVPEDSYLMLGDNRNDSW
                     DARYWTNPYVREDKILGKAAVIYWPVHDIGKLE"
     gene            4923..6089
                     /locus_tag="ACZO9R_01350"
     CDS             4923..6089
                     /locus_tag="ACZO9R_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR0911752.1"
                     /translation="MVTLLAKIFIQEKNDQAKIREEYGVLCGVVGILFNVLLFAGKFL
                     AGTISHSIAITADAVNNLSDAGSSIVTLAGFKLAGTKPDPEHPFGHGRIEYISGLVVA
                     AAILLMAYELIRDSVGKIVHPEETEFSGLIVVILVLSILVKLYMYFYNHMIGKKLDSA
                     AMQATAIDSLSDTCATTAVLAATLIGHFTGLHIDGWCGVLVGVFILYAGISAAKETLN
                     PLLGQPPAEDFVMQIDRIVMSHPEICGIHDLIVHDYGPGRKMVTLHAEVPADGDILEV
                     HDVIDNVENELREKLGCEATIHMDPIVTSDEHVSELKAAVVSITKGIDDVITIHDFRV
                     VSGPTHSNLIFDVLVPFQFKMSDEELVAAICGRVTECLGDNYYPKIKVDHAYVK"
BASE COUNT         1708 a         1270 c         1901 g         1411 t
ORIGIN      
        1 agagagtgga cggattgtcc gattctcgtt gtatccggac agacgggata tatggataaa
       61 gtcaatgccc tgtatgcggg agcggacgac tacattgtca agccgtttca tgagcaggag
      121 cttgcggcga gaatttacac tgcgctgcgc agaaggattc cgggaggaca gggaagcttt
      181 tatcaggcag gagatctgaa gattgatttt tcgagacggc aggtaagcct tgccggggag
      241 gagattcatt tttcgccggt agaataccgg attctggagt gtcttgccct caattcggga
      301 actgtggtga cgtatcagat gttgttagag aagatatggg gaccgtatgc aagcagcggc
      361 agccgtattc tgcgggtcaa tatgacaaat atccgcaaga agatagagaa gatcccgatg
      421 gatccgacgt acattaccac gatcccgcgt gtgggctacc gcatgcttga gagtcagacg
      481 gcatcggttg tctgagctga ggttgaagcg gaagctttgg atgatgaagt gcctttgcct
      541 tcaaaatagg aatcatagat tccgtagata aaacctccga taatgataag aataccgatc
      601 acagaaaaaa tgtgttcaaa gattgtcata taaaatcccc ctttggtttc agttgtcatc
      661 aagtataaca gccaggaaaa cggaggggga ttttgccgct tttttttaat tttttcatag
      721 tgtaccagga agagacttaa cactttttta gcagcgggag ccaggcagag gcaatattgt
      781 atgctttttg aacaaaatgg ctatattaaa taaagcactt tttcgtagaa atatatggaa
      841 atatttgtgt tttttgctat aataagggta atatgttaag aaacagggag aaggttatca
      901 atatgaaaaa ggggaagaag caggcaaagc gaagaaacgc gaatgttacg atggcatttg
      961 gaatccggga gaagatcgtg ctgtgttttc ttgtgccggt tctgtttatg attgtgcttg
     1021 gaattatttc ctaccgcaag gcggcggacg gaatgagcag ttcctatcgc gattccacgc
     1081 agcagacgat cgagatggcg ttgcagaata tcgatacgag caactcgttc atcagtgcag
     1141 aggcattaaa atacgtggtg gattcggatc ttggaaagta ttttgtgggg atttacgaca
     1201 atgatccgac gtccaagcgg acgttgattg accaggtgaa gacaagaatt cttgcttccc
     1261 aggttggcaa ctcctatatc agcaacatct atattattcc ttccggcgat ctgcagatga
     1321 tctcgacgaa gaacaaggcg cagaagggca tttaccagga gtacatggat gagatggcgg
     1381 ttgatggcaa gctgggacag tgggatgacc atcacaatgc gctggatgag tatctgagca
     1441 tcgactcgtc cagatacatt atggtatatc aggcagccgc gcagaacggt aaggcggtta
     1501 tcgtaatcga catcaaggcg gaggcagtca cagagttcat gcagggactg aatctgggag
     1561 acggaagcat tgtgggattc gtgacggcag gcggcagaga gcttgcagtg aagcgcaatg
     1621 cgggagacga acagggaacg ccggtcgagg gagagacgat ttttgcagac aaggattttt
     1681 atctgcaggc gagtgagacg gacggcgtaa gacaggtgca gtacaacgga acagagtatc
     1741 tgtttttcca cagcaagagt gaggataccg gggcaacgat gtgtgcactg gtgccgttaa
     1801 aggtcattac cggacaggca gagtccatta agcagatcac gatagcggga gttctgatct
     1861 ccagtatcat tgcacttttg atcggaaccg cagtgacaag cggaatccgc aagaatatga
     1921 agcgtatttc cggaagcctt gaggttgtgg cggaaggtaa tctggcaacg accgtgtccg
     1981 tgaagggacg cgacgagttc cgcggacttg ctgctgcggc aaatgacatg atcgcacaca
     2041 acaagcagct ggtgcagaag gtcagccagg cgacggataa gctggcggca tctgccggtg
     2101 aagtgacgaa tgcatccggt attattcagg agtattcgca ggacatagca aacgcgatcg
     2161 gtgagatcaa cgagggcatg gagcggcagt ccacacatgc gcaggagtgc gtgagcaaga
     2221 cgggaacgct ttccgaggag attcaggaag tcaaccgtgt ggcgcgcgag gtagagacgc
     2281 ttgtgtctga ggcggaggag atgatccgca aaggtatgga ccggatcgaa cagctgggac
     2341 agcgcgcgaa tgagacgaca gctgtgaccg cgaaggtgga agaaagcatc gaagacttga
     2401 agcgcgaatc ggagacgatc aatcagtttg tttctgtcat tacagatatt tcggagcaga
     2461 cgaacctgtt atcgctcaat gcatcgattg aggcggcaag agccggagag gcaggacgcg
     2521 gatttgcggt tgtagccgag gagatccgta agcttgcaga caattcggcg gaggctgccg
     2581 gagagatccg caataatgtg gagaatatca gtgcgcagac cggcgtgagc gtcgagagcg
     2641 caaagcaggc gggcgagatg gttgcacagc agaccgaagc agtgcgtgag gttatcggtg
     2701 tattccagga tatgagcgat gccatggata agctgtttga cggattaaag gagattcttg
     2761 ccggaacgga gcgtgccgat aaggagcgcg aggatacgct tgaggcagta cataatattt
     2821 cttctatcat tgaggagacg gcagcaagcg ccgaagtggt gaagaaggtt gccgcaagtc
     2881 tggagcagaa tgtagagaac ttaaacggca cggcggagag cctgggagat aatatgacag
     2941 gtcttaagac cgagatcgcc gtatttaaga cagaataggc gaagaacatt tagagaatat
     3001 aatagaagat aaaaaagcgg cagccagtcg atcatgagat cggctggctg ccgcttcttg
     3061 aacgcagaga gaactgcatt ttataaagca aaatttgcag gggtcacact gcatcagaac
     3121 gcgcaatcag aggttcaggc aggaaagatt aagcagaaac tgccggtgca gcagctctct
     3181 ggagagtaca gcagactgaa aggagtcaag cgcaagctga tagacattcg catactgtac
     3241 atagtgccag tgaagttccg tctgccagtt gtcgagtgtg atcaggcggt cttcatccag
     3301 atgaaaatca aacagatggc tgcgcagctc gtaaagggcg gcatagcgga acaggttggc
     3361 gagcatatgg catgccttat ctgtgtcagt gatggcaggc agacgcacgc tgccatagcg
     3421 cttaagccct gcaaaccggg agccggttcc ggaaaggttc tgggcgatct cggtctcgag
     3481 aagtgtgcca aaatcggtgt tgtagtagtt taaggtggca gcgaccaggg tatccgtcaa
     3541 ctggacaaag gacggatggt gcagacagtc atgctgctca aaatactcgt ccataaagtc
     3601 atagagaaca aaagactcgc agctgaagtg atattccgga cggtatgcat ttaaaaactg
     3661 cggcagcaaa acagccagtg cgtccgtgtg atgcatcagg cacaggcaga gtgctgcgta
     3721 gaagttccgg cagtccacga gcagggcatc cagcggcaga ttgccgtctg cgtaaggcag
     3781 gctggcagcc tctggcgcat gatcttctat cagagagttg ataactggtg tggtggtgcc
     3841 gaaaggaacc cctgcagtca gcacaatggc ggcatacaga taaaacggtt caaagccgtc
     3901 cgctgcgatg gaaggcgaga aatagtcgtc tgcagagaga taatcctcca gtatctcaaa
     3961 gctgtcctcg gccgacgggc aggaaacctg atctgcgagg aattgtgcga gatccggatg
     4021 ctcttctgac acgcaggata gaaaacgaaa gagcgagtag gtgagaacac gctgtctgcc
     4081 ggcaggctcc ttcgtgtagt ttacaagaaa tgcgggggga aataattgat cgttataagt
     4141 ttccatgaag aactcctttt ccaaaagtaa tccgtatgtg tgtgcggacg gatgcccgca
     4201 gcgcataccg acatcatagc acattttaga attagatgcc atcatgcgtt ttcattttcc
     4261 gtcaggtgtg ttacaataac aagcagacat agaaagcaga cagagggaaa aagatgccaa
     4321 aacagaatca accaaaggaa gagaaaagag aaaaggggac agcacaagcg gtcatccgtg
     4381 agattttaag ctgggtgata ccgtttgtaa tcgcgcttgc cgttgcgatg tttttgaaaa
     4441 attatattat catcaatgcc gatgtgccga ccgggtcgat ggagaacacc atcatgccgg
     4501 gggatcgtct gatcggaaac cgtctggcat accggagcaa gtcgccggag cgcggtgata
     4561 ttgtcatttt ccgttatccg gacaacgagg aagaactgta tgtgaagcgt gtgatcgggc
     4621 ttccgggcga gaccgtggat atccgggacg gatacattta tattgacgga gcggatacgc
     4681 cgctggacga ggattatctc aaagaggact ggacggtggc gacggggaat tatcattttg
     4741 aggtgccgga agactcttat ctgatgctgg gggataatcg gaatgattcg tgggacgcac
     4801 gatactggac gaatccgtat gtgagagagg ataagattct gggcaaggcg gcagtgatct
     4861 actggccggt gcatgatatt ggaaagcttg agtaggggag aagcgatcga gggggtagtt
     4921 ttatggttac attattggcg aagatattca ttcaggagaa gaacgatcag gcgaagattc
     4981 gtgaagaata tggtgtgctg tgcggtgtgg taggtattct gttcaatgta ctgctctttg
     5041 cgggaaaatt tcttgcgggt accatcagcc attccattgc catcacggca gatgcggtca
     5101 acaacctgtc ggatgcggga tcttcgatcg tgacgctggc gggcttcaag ctggcaggca
     5161 caaagccgga cccggagcat ccgttcggac atggcaggat tgagtatatc tcggggctgg
     5221 tggtggcagc agcgattctt ctgatggcat atgaattgat ccgggattcg gtgggcaaaa
     5281 tcgtacatcc ggaggagaca gagttctccg gactgattgt tgtgattctg gtgttgtcga
     5341 ttctggtgaa gctgtatatg tatttttaca atcacatgat cgggaaaaag cttgattccg
     5401 cggcaatgca ggcaacagcg atcgacagcc tgagtgatac ctgcgcgaca acggcagttc
     5461 ttgcggcaac actgatcgga cattttacag gacttcacat cgacggatgg tgcggtgtgc
     5521 tggttggtgt attcatcctg tatgcgggga tcagcgcggc gaaggagaca ctcaatccgc
     5581 tgttgggaca gccgccggca gaggattttg tcatgcagat cgaccggatt gtcatgtcgc
     5641 atccggagat ctgcgggatc catgatctga tcgtgcacga ttacggcccg gggcgcaaga
     5701 tggtgacgct gcatgcggaa gtgccggcag acggcgatat tctggaagta catgatgtga
     5761 ttgacaacgt agagaatgaa ctgcgggaga agctggggtg tgaggcgacg atacatatgg
     5821 acccgattgt gacctccgat gagcacgtca gtgaactgaa agccgctgtc gtatcgatca
     5881 caaagggaat agatgatgtg attacgatcc atgatttcag ggtagtcagc ggtccgaccc
     5941 attccaacct gatcttcgat gtgctggtgc cgttccagtt caagatgagc gatgaggaac
     6001 tggttgcggc gatctgcgga agagtcacgg aatgcctggg ggataattac tacccgaaga
     6061 ttaaggttga ccatgcctat gtaaaataaa acgaagaaga ttgcggatgt aaaggaattg
     6121 ccgcaataag cagagtcctc tttggagtga taagtaatat tacaggaagc gcatgatgca
     6181 gatttcgggt ggaactgcat atgaaacatt cgttggagaa aaatgtgtgt atttcttcca
     6241 gccggatgac tgcatctgat tccgtgtctc ttccacgttt ggactctccc
//
LOCUS       DBMDQJ010000037         6219 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5116_length_6219_cov_1.615397, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000037 DBMDQJ010000000
VERSION     DBMDQJ010000037.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5116_length_6219_cov_1.615397"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(549..2435)
                     /locus_tag="ACZO9R_01355"
     CDS             complement(549..2435)
                     /locus_tag="ACZO9R_01355"
                     /inference="COORDINATES: protein motif:HMM:NF014069.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR0911753.1"
                     /translation="MISNTDIRYFFDTYYDNFMHSLAWEDHLLEPASREEWLATLRAC
                     ADDQQQRCADNQILLEQYLYPLSSNPALLSDEGYDLLLSFCRDFYYSNYTEPALLIRI
                     IDILLPHYEDRSDTESLLFLYICAGFSYMELSRTGEPTSRDKTIYFYTKVLSYRDQIE
                     FFESAASRDYIFIAYYNLIRVAPGLGILDIETAYRLWEELCQIRTMPKFCQYDTTNPR
                     IPQLCDCAINDFRAYGAVLNVERVIAPSAILSSLENMTQIYYSEILHEHGSIYECPVF
                     TVFNYHYVQARNGTITWEAAWQILDDYYVHKMQTIGQVSSPDLLAFYTNLPLNLIDVL
                     GHTHFKEEFKQATYRRYRNLITSYLMNQPASLNSYTLNNGVQLVAFHPLILGTFQNRM
                     EKMNFIVDLVVTRHLTTFTHSVMVSYLAEAIGKHILAARPELLRIPSSYLEGNVYASP
                     AEVLDYIVPAALFHDIGKNGMIPIINTQHRKLNDYEFNIIQTHPQKGSDYLSSDPDFL
                     IYQPVALGHHRSYDGTRGYPLSFDNTASPYRDAIDLIHICDCLDAATDYLSRNYHRAK
                     PFDVVLNELKAGRGTEYNPDMVDVILSDRELYNDLKMLTEQNRENIYYDIYLTFVNLR
                     KKRQ"
     gene            2697..3284
                     /locus_tag="ACZO9R_01360"
     CDS             2697..3284
                     /locus_tag="ACZO9R_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR0911754.1"
                     /translation="MSNTTKLALEASLKKLLLKKPVDKITINDLTEDCGISRMAFYYH
                     FKDIYDLIEWACLEDGKRALADKKTYSTWSEGLEQIFEAVLENKPFILNVYHSVSQKK
                     IESYLYKLTYDLLADVVEEECRDAAVSGEDKALIAEFYKYGFVGMMLHWIDGGMKADY
                     RELVQRLSTMLHGSILNAARNFEQKQSASLSKETE"
     gene            3392..5368
                     /locus_tag="ACZO9R_01365"
     CDS             3392..5368
                     /locus_tag="ACZO9R_01365"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HIR0911755.1"
                     /translation="MGKGKHIGLGVAALAAGAGVAALAAGSHKNSSERAQKKAIEEKA
                     RAEYRNTERGKHEKNSKGIYYTNGNYEAFARPRKPEGVDEKSAYIVGSGLASLAAACF
                     LVRDGQMEGSHIHILEAMDIAGGACDGINDPTRGYVMRGGREMENHFECLWDLFRSIP
                     SIETPGVSVLDEYYWLNKEDPNYSLCRATEERGKDAHTDGKFNLSQKGCMEIMKLFMT
                     KDEDLYDKTIEDVFDDEVFNSTFWLYWRTMFAFENWHSALEMKLYFQRFIHHIAGLPD
                     FSALKFTKYNQYESLILPMQRYLEEAGVDFQFNTEVTNVIFDIKDDKKVAKALECKVN
                     GVEKGIVLTENDLVFVTNGSCTEGTIYGDQNHAPNGDAEVRTSGCWSLWKNIAKQDPS
                     FGHPEKFCSDVAKTNWESATVTTLDDKIIPYITNICKRDPRSGKVVTGGIVSCKDSSW
                     LLSWTINRQGQFKDQDKDKVCVWVYGLFTDVPGDYVKKPMKECTGKEITEEWLYHLGV
                     PVEEIPDLAENSAVCVPTMMPYITAFFMPRTKGDRPDVIPDGCVNFAFLGQFADTPRD
                     TVFTTEYSVRTAMEAVYGLLGVDRGVPEVWGSVYDVRELLDSSVKLMDGKSPLEIELP
                     GPLNVLKKPLLRVVKGTVVEKLLRDHDVIKDGML"
     gene            5538..5619
                     /locus_tag="ACZO9R_01370"
     tRNA            5538..5619
                     /locus_tag="ACZO9R_01370"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5572..5574,aa:Tyr,seq:gta)
     gene            5652..5957
                     /locus_tag="ACZO9R_01375"
     CDS             5652..5957
                     /locus_tag="ACZO9R_01375"
                     /inference="COORDINATES: protein motif:HMM:NF016211.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HIR0911756.1"
                     /translation="MVLFCFSQSSTRLHDWFTGTDLYKKNLESYVRKEGMQMKTKVSI
                     VATVTLLMAIGFICMKNVPIGRIVLSVVWVCHVIYFFCFVKTLPEEKTGSGGIEYEE"
     gene            complement(6052..>6219)
                     /locus_tag="ACZO9R_01380"
     CDS             complement(6052..>6219)
                     /locus_tag="ACZO9R_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HIR0911757.1"
                     /translation="EILPALMPKVIAKVCQSTSTPVIAGGLVSEKEDILALLDAGATS
                     ISSTNEKIWFL"
BASE COUNT         1665 a         1308 c         1750 g         1496 t
ORIGIN      
        1 ctaactaaaa atatttgaaa tgggatatgt tttattataa gaatatcgga gaaaatgaag
       61 ttccagaatg caaccggggc ggcaattctt tgaagcaaaa tccgaggatt tcttccgtgc
      121 cgcgtcggac atcgggttct gtgtctttcc acgtttggac agtccccggg gtgtgtccgg
      181 caaaaccgca gacaatgagc ggactttctg gtgcccctag tggcaccgct tcgcagacaa
      241 tataaaaccg cgcgaaccga actcgttatc atctgctatt gaaaacaggg cagatgacaa
      301 ctcagacagc ggcttcgcgc ggttatgttt tactcagtgg tgccaacaag gggcacacac
      361 aaaagtcctt aaattgtctg cggtttgcgg acacaccacg tggaatgttc caaacttgga
      421 aaaacacaga tgatcaggga tgtccgacgc ggcatggtta gaaataccgg attttgtgat
      481 tagaattgcc gccccggttg catctggaac gtgatagtct ctggttctta taataaaacg
      541 tatcccattt attgtctttt cttgcggaga ttcacaaacg tgagatagat atcatagtag
      601 atattttcgc ggttctgctc cgtaagcatc tttaaatcgt tatataattc ccggtcgctt
      661 agaatcacat ccaccatatc aggattgtac tcggtgccgc gccctgcttt cagttcgttc
      721 aataccacat cgaacggttt cgcgcggtgg taattgcggc ttaagtaatc ggtcgctgca
      781 tccagacagt cacagatgtg gatcaggtcg atggcgtccc ggtacggcga ggcggtattg
      841 tcaaaactga gcggataacc gcgggtaccg tcgtaggagc ggtgatgtcc gagcgcaacc
      901 ggctggtaga ttaaaaagtc cggatcggac gacagatagt cggagccctt ctgcggatgt
      961 gtctgaataa tgttgaattc gtagtcgttt aacttgcggt gctgcgtgtt gatgatcggg
     1021 atcatgccgt ttttcccaat atcgtggaac aaagctgcgg gaacgatata gtccagtact
     1081 tccgcggggg aggcatagac atttccctcc agataggagg acggaatccg caaaagctcc
     1141 ggccgtgcgg ctaaaatatg ttttccgatc gcttctgcga gataggaaac cataacggag
     1201 tgtgtgaatg tcgtgagatg tctggttacg accagatcta cgataaagtt cattttttcc
     1261 atacggttct ggaaagtccc gaggatcagc gggtgaaacg ccaccagctg gacaccgttg
     1321 tttaacgtgt aggaattgag actggcaggc tgattcatca gatagctggt aatcagattg
     1381 cggtagcgcc ggtaagttgc ctgcttaaac tcttccttga aatgcgtatg ccccagcacg
     1441 tcgatcaggt tgagcggcag attggtataa aaagcaagca ggtcaggcga ggaaacctga
     1501 ccgatggtct gcatcttgtg gacgtaataa tcgtccagga tctgccaggc ggcttcccag
     1561 gtgattgtac cattgcgtgc ctgcacatag tggtaattaa atactgtgaa tacagggcac
     1621 tcatagatgg aaccatgctc gtgcaatatc tcggagtaat agatctgcgt catattctcc
     1681 agagaggaca ggatcgcgga tggtgcaata acacgttcaa cgttaagaac agcgccgtac
     1741 gcgcggaaat cgttgattgc acagtcacag agctgcggaa tccgtggatt ggtggtatcg
     1801 tactgacaga atttgggcat ggtacggatc tgacagagct cctcccagag ccggtaagcg
     1861 gtctcaatgt ctaagatgcc aagacccggc gccacccgga tcagattgta ataggcaata
     1921 aaaatatagt cgcggcttgc agctgattca aagaattcaa tctgatcgcg gtaagagagc
     1981 actttggtgt agaaatatat agtcttatca cgagaggtgg gctctccggt tcgtgagagc
     2041 tccatgtatg aaaagccggc gcagatatag agaaaaagca ggctttccgt atcgctgcgg
     2101 tcttcataat gtggaagcag aatatcaata atgcggatca gcagagccgg ttcggtatag
     2161 ttgctgtagt aaaaatcacg gcaaaaagaa agtaaaagat catatccctc atcggacaga
     2221 agagcaggat tgctgcttag tggatagaga tactgttcaa gcagtatctg attgtccgca
     2281 cagcgttgct gctggtcgtc tgcacatgcg cgaagtgttg ccagccattc ctcacggctg
     2341 gcaggctcca gaaggtgatc ttcccaggcg agactgtgca tgaagttgtc atagtaagta
     2401 tcaaaaaagt aacggatatc cgtgttgctg atcatgtatt cctccgcctg cgcatttaaa
     2461 tatgtgtgaa aaccacccaa tttgtccata agcgcaaata taataatatt ataacaaata
     2521 gatcataaat gtatatagta atttggatat gcaggtggtc aaaaacgtgc atcaacggct
     2581 aaaaaagaat taagaaaatt tagaaattct ttatttgccc ctgcctgtga ggaggaatac
     2641 aataaaggtg cggacggtaa gatccgttgc cgcaagaatg gaacagggag gcagccatgt
     2701 cgaacacgac aaaacttgca ctggaggcat cacttaaaaa gcttttgctt aagaagccgg
     2761 tggataagat tacgatcaac gatctgacgg aggactgtgg gatcagccgc atggcgtttt
     2821 actatcactt caaggatatt tacgacctga ttgagtgggc gtgcctggag gacggaaagc
     2881 gtgcgcttgc ggataagaag acgtacagta cgtggagtga aggactggag cagatctttg
     2941 aggcggtatt ggagaacaag ccgtttattt tgaatgttta tcactcggtc agccagaaga
     3001 agatcgagag ctatctctat aagctgacct atgatctgct cgcagatgtg gtagaggaag
     3061 aatgccgcga tgcggctgtc agcggggagg acaaggcact gattgcggag ttctacaaat
     3121 acggctttgt aggtatgatg ctgcactgga tcgacggcgg tatgaaggcg gattacagag
     3181 agcttgtgca gcggctgagt acgatgctgc acggcagtat cttaaatgcc gcccggaatt
     3241 ttgaacagaa gcaatccgca agcttatcaa aagagacgga atgataagtt ttttatcatt
     3301 ttgatctttt tgtaaattgt gtcattcatc tgggcagact aagataagga cacaaagaat
     3361 ttacagcata agtggaaagg tggtagttac gatgggaaaa ggaaaacata taggacttgg
     3421 cgtggctgcg ctggcagccg gagcgggtgt ggcggcactg gcggcaggca gccataagaa
     3481 cagcagcgag cgtgcgcaga agaaagcaat cgaggagaag gcgagagcgg aataccgcaa
     3541 taccgagcgc ggcaagcatg agaagaacag caagggtatt tactatacca acggtaatta
     3601 cgaggctttt gcgagaccga gaaagccgga gggtgtggat gagaagagcg cctatattgt
     3661 cggcagcgga ctggcctctc tggcagcagc ctgcttcctg gtgagagacg gtcagatgga
     3721 aggttcccac attcacattc ttgaggcaat ggacatcgcg ggaggcgcct gcgacggtat
     3781 caatgatccg accagaggat atgtgatgcg cggcggacgt gagatggaga accattttga
     3841 gtgtctgtgg gatctgttcc gcagcattcc gtcgattgag acgccgggcg tctccgttct
     3901 ggacgagtat tactggctga acaaggaaga cccgaactat tccctgtgcc gtgccacaga
     3961 ggagcgcggc aaggatgcgc acacagacgg caagttcaat ctgagccaga agggctgcat
     4021 ggagatcatg aagctgttca tgacaaagga tgaggatctc tacgacaaga cgatcgagga
     4081 tgtgttcgac gacgaggtat tcaattctac gttttggctg tactggcgca cgatgtttgc
     4141 ttttgagaac tggcacagtg cactggagat gaagctctat ttccagcgtt tcatccatca
     4201 catcgcggga ctgccggatt tcagcgcttt gaagttcacg aagtacaatc agtatgagtc
     4261 gctgattctg ccgatgcaga gataccttga ggaagccggt gttgacttcc agtttaacac
     4321 cgaggtaacg aatgtcattt tcgacatcaa agatgacaag aaggttgcca aggcgctcga
     4381 gtgcaaggtc aacggtgtgg agaaaggtat tgtgcttacg gagaatgatc tcgtgttcgt
     4441 gacaaacgga agctgtaccg agggtacgat ttacggtgat cagaaccatg cgccgaacgg
     4501 ggacgcggaa gtgcgcacga gcggctgctg gagcctgtgg aagaatattg caaagcagga
     4561 tccgtctttt ggacatccgg agaagttctg ctcggatgtg gcgaagacga actgggaatc
     4621 tgcaaccgtg accacgttgg acgacaagat cattccgtac atcacgaata tctgcaagcg
     4681 tgatccgaga agcggaaaag ttgtgaccgg aggtatcgta agctgtaagg attccagctg
     4741 gctgctcagc tggacgatca accgccaggg acagttcaag gatcaggata aggataaagt
     4801 atgcgtgtgg gtatacggtc tgttcaccga tgtgccgggc gattatgtga agaagccgat
     4861 gaaggagtgt accggtaagg agatcaccga ggagtggctg tatcatctgg gtgttccggt
     4921 ggaggagatc ccggatctgg cagagaacag cgccgtatgt gtaccgacga tgatgccgta
     4981 catcacagcg ttctttatgc cgagaaccaa gggcgaccgc ccggacgtca tcccggatgg
     5041 ctgtgtgaac tttgcgttcc tcggacagtt cgcggacaca ccgcgtgata cggtcttcac
     5101 gacggaatat tcggtcagaa ccgcaatgga ggcggtatac ggactgctcg gtgtagacag
     5161 aggcgttcct gaggtatggg gaagtgtcta tgatgtcaga gagctgctcg acagcagtgt
     5221 gaagctgatg gatggaaagt ctccgcttga gatcgaactg ccgggaccgc tcaatgtatt
     5281 gaagaagccg ctgcttcgtg tggtcaaggg aaccgtcgtg gagaagctgc tccgggatca
     5341 tgatgtgatc aaggatggta tgttataggg aatcctgatg tgtagaaaac tctgatttcc
     5401 tgaatgaacg gcaacgcccg gaaagccttt ggttttccgg gcatttattg aaaataggtg
     5461 acacttttta aatttataac ttgcatatct gtttaggctg tgatataatt tacctctgtc
     5521 aggacacatt gacaaatggg cagattcccg agtggccaaa ggggacagac tgtaaatctg
     5581 ctgcaaattg cttcggtggt tcgaatccac ctctgcccat gaattgttga aagaaccatt
     5641 gccgtaaggc agtggttctt ttttgctttt cacaaagttc aacgcgcctt catgactggt
     5701 ttaccggtac cgatctttac aaaaagaatc tggaatccta tgtgagaaaa gaaggtatgc
     5761 agatgaaaac caaggtcagt atcgtcgcca ctgtgacgct tcttatggcg atcggattta
     5821 tctgtatgaa aaatgtgccc atcggacgga ttgttttatc cgttgtgtgg gtatgtcatg
     5881 tcatctactt tttttgcttt gtaaagacac ttccggaaga aaaaacagga agcgggggaa
     5941 tcgaatatga agaataaacg cagcctgcat ttttgtaatc ctgctggtgg cgcatatggt
     6001 ccagcacaga atgtctggca ctgcatcata atacatgcag gagaggaatg cttataaaaa
     6061 ccagattttc tcatttgtgg aggagatact ggtcgcgcct gcatccaaaa gagcgaggat
     6121 gtcttccttt tcggagacca gcccgccggc aatgaccggt gtggaggtgg actgacagac
     6181 tttggcgatg accttgggca tcagtgccgg cagaatctc
//
LOCUS       DBMDQJ010000038         6166 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5168_length_6166_cov_1.609893, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000038 DBMDQJ010000000
VERSION     DBMDQJ010000038.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6166)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6166)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6166
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5168_length_6166_cov_1.609893"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="ACZO9R_01385"
     CDS             <1..489
                     /locus_tag="ACZO9R_01385"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlgC family sugar phosphate
                     nucleotidyltransferase"
                     /protein_id="HIR0911758.1"
                     /translation="ERWDFVTDILIRYKNMKQIYGYKMKEYWSNIATVESYYQTNMDF
                     LKPEVRKYFFHDEPKIYSKVDDLPPAKYNIGSDVRNSLIASGCIVNSKVENSILFKKV
                     FVGKNCVIKNSIILNDVYIGDNTHIENCIVESRDTLKANTYYCGDNGIKIVSENNERY
                     VL"
     gene            517..783
                     /gene="spoVG"
                     /locus_tag="ACZO9R_01390"
     CDS             517..783
                     /gene="spoVG"
                     /locus_tag="ACZO9R_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HIR0911759.1"
                     /translation="MQITDVRIRKVEKEGKMKAVVSITIDEEFVVHDIKVIEGDKGLF
                     IAMPSRKAADGEYRDIAHPINSDTRERIQTLILQKYQETMAAEE"
     gene            1010..1771
                     /locus_tag="ACZO9R_01395"
     CDS             1010..1771
                     /locus_tag="ACZO9R_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR0911760.1"
                     /translation="MSQLKAVILAAGKGTRMKSDLPKVVHTIEGKCLVDYAIEAAEGA
                     GAGEICLVVGYKYEIVRETISHKDVKFVLQEQQLGTGHAVRCAKDFLGEEGQTLILFG
                     DTPLITAETLKKLREHHIASGNTVTVLSAMVEDPTGYGRIIRDADGNFVKSVEHKDAT
                     EAELASHEINSGMYIFDTKELREALDLIQPNNAQGEYYLPDTLTIIKEKGLKVDAYAL
                     DDPEDITGVNDQEQLAAAAVVIRRRIAGNKAKNRR"
     gene            1774..2358
                     /gene="pth"
                     /locus_tag="ACZO9R_01400"
     CDS             1774..2358
                     /gene="pth"
                     /locus_tag="ACZO9R_01400"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR0911761.1"
                     /translation="MFLIVGLGNPGRQYEHTRHNAGFDVMDALAEKYNISISESGHKA
                     LFGKGMIGGQKVILAKPQTFMNLSGESVAELLHYYKIDPEEELLVIFDDISLAPGNIR
                     IRKKGSAGGHNGIKNIIAMTGTENFKRIKVGVGEKPAGWDLADHVLGRMSEEDRAAFE
                     EAVKESVKAAEMILEDEIDQAMNDFNRKKRESQS"
     gene            2355..>4910
                     /locus_tag="ACZO9R_01405"
     CDS             2355..>4910
                     /locus_tag="ACZO9R_01405"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000716 - transcription-coupled
                     nucleotide-excision repair, DNA damage recognition
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR0911762.1"
                     /translation="MKTFTQPMQGLKEFEDLEAALPKLSGVIQISGCIDAAKPHMMYS
                     IGNGSGNRIIVTFQEQKAKELAEEYRFFDPNVAYYPAKDILFYQSDIRGNVLTAERIN
                     ALKMMAEERSCTIVTTFDGLMNPMPSPDQFISAVRKVAVGDVIDLEQLTRHLAELGYE
                     KNYQVQTSGEFSVRGGIIDIFPLTEENPFRIELWDDEVDSIRSFDAESQRSIENLDEV
                     HIYPACELVLTKEERDAGIARILAEAKQVSEKLRGEMKTEEAYRVKSAADQVAEEAGE
                     LGLSAGLDAYLSYFCEERVSLLDYFGKQDTLVFLDETPRSIERGMATETEFSESMKQR
                     LEKGYILPGQMRELFTCKEILGKLAGRRCISLVALDLKNPHLEIGTRLNIQSKTVNPY
                     NNSFELLIKDLERYKKNGYQVILLSGSRTRAKRLADDLMAEGLNAFYSEDYDHEVKPG
                     EIMTGYGKIKKGYEYPMLKFVVISESDIFGGEKKKKKRKRIYEGEKIASFTDLNIGDY
                     VVHESHGLGIYRGIEKIEVDKTEKDYIKIEYAGGGNLYILATQLEQIQKYAGAGAKKP
                     KLNKLGGQEWNKTKSRVKGAVREIAEDLVKLYAVRQNDRGFAFGPDTVWQKEFEEMFP
                     FEETEDQDLAIAATKADMESTKIMDRLICGDVGYGKTEIAIRAAFKAVQDGKQVAFLV
                     PTTILAQQHYNNFVQRMKDFPVNIDLLCRFRSSAEQKKTIEKLKKGQVDIIIGTHRLL
                     SKDVVYKDLGLLIIDEEQRFGVAHKEKIKQLKTNIDVLTLTATPIPRTLHMSLIGIRD
                     MSVLEEPPMDRVPIQTYVMEYNEELVREAISRELARGGQAYFVYNRVREIADVA"
     assembly_gap    4911..4920
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4921..5873
                     /locus_tag="ACZO9R_01410"
     CDS             <4921..5873
                     /locus_tag="ACZO9R_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HIR0911763.1"
                     /translation="AHGQMKETELENIMYRFINGEIDVLVSTTIIETGLDISNVNTMI
                     IHDADNMGLSQLYQLRGRVGRSNRTAYAFLMYKRDKMLKEVAEKRLAAIKEYTELGSG
                     FKIAMRDLEIRGAGSLLGERQHGHMEAVGYDLYCKMLNEAVKEAKGIAVEESFDTSID
                     IVIDAYIPMGYIPNELQKLDIYKRIADIETQEETEEMTEELIDRFGDPPKSVENLLYI
                     AKIKSMAHRLYFTEVAQKGESVRFTLYEKARIDVTKIPELVAAYGQKVTFTADPKNPY
                     FTYHLKKNSRDKNVDIKSVVEQFLADAGGFLAEREAPKEG"
BASE COUNT         1751 a         1257 c         1889 g         1259 t
ORIGIN      
        1 gaacgctggg attttgtgac ggatattctg atccgctata agaatatgaa acagatctat
       61 ggttataaga tgaaggaata ctggagcaat attgcgaccg tagaatccta ctatcagacg
      121 aacatggatt tcttaaaacc ggaggtgcgg aaatactttt tccatgatga gccgaagatt
      181 tactctaaag tggatgatct cccgcctgca aaatacaata tcggttccga tgtgcgcaac
      241 agtttaatcg ccagcggatg catcgtcaac agcaaggtgg agaattcgat cctgttcaag
      301 aaggtatttg tcgggaagaa ttgtgtgatt aagaactcca tcattctgaa tgatgtttac
      361 atcggcgaca acacacacat tgagaactgc attgttgaga gccgtgatac actcaaggcg
      421 aacacctatt attgtggtga caacggtatc aagattgtat ccgagaacaa cgaaaggtat
      481 gtactgtaga gaagggacta aagggggaca gaatctatgc agattacgga cgtgagaatc
      541 agaaaagtcg agaaggaagg caagatgaaa gcggtggtgt ccatcactat cgatgaggaa
      601 ttcgtggtac acgacatcaa ggtgatcgag ggagacaagg gattgttcat cgcaatgccg
      661 agcagaaagg ccgcggacgg ggaataccgc gacatcgctc atccgatcaa ttcggacaca
      721 agagagcgga tacagactct gatccttcag aaataccagg agacaatggc tgctgaggaa
      781 taagaccggg agaaagccgg atggcagttt acccagggaa ataaatgcgc acaggtttta
      841 cgggaaggta aagcctgtgc gcatttgttt atacgtgcag ggatcgggca gtggtgcaga
      901 aaactcttgt aaaatctgca aactaaggta aaatgaaacg tgaactgtga caagatcaaa
      961 caaaagacag caggaatcgt caattacaat agatggcagg aggagtatga tgagtcagtt
     1021 aaaagcagtg atacttgcag cgggaaaagg cacacgaatg aagtcggatc tgccgaaggt
     1081 ggttcatacc atagagggaa aatgtctggt ggattatgcg attgaggcgg cagaaggagc
     1141 cggcgccggt gagatctgcc ttgtggtcgg ctacaaatac gagatcgtgc gggagaccat
     1201 ttcacacaag gacgtaaagt ttgtattaca ggagcagcag ctgggaaccg gtcatgcggt
     1261 gcggtgtgca aaggattttc tcggggagga aggacagaca ctgatcctgt ttggagatac
     1321 cccccttatc acggcagaga ccttaaaaaa attaagggag catcacattg cgagtggaaa
     1381 tacagtgaca gtgctctcag caatggtcga ggatccgacc ggatacggca gaattatccg
     1441 agatgcagac ggcaattttg tgaagagcgt ggagcacaag gatgcgacgg aggcggaact
     1501 ggcatcccac gagatcaact caggtatgta tatttttgat acgaaggagc tgcgtgaggc
     1561 gttggatctg attcagccga ataacgcgca gggtgaatac tatctgccgg atacgctcac
     1621 aattattaag gaaaagggac tgaaggtgga tgcgtatgcg ctggatgacc cggaggatat
     1681 cacaggggtc aacgatcagg agcagcttgc ggctgcagca gtcgtgatcc ggcggagaat
     1741 cgcaggcaac aaagcaaaga acaggagata gcgatgtttt tgatcgtagg tctggggaat
     1801 ccgggcaggc agtatgagca tacacgccat aatgcgggat ttgatgtcat ggatgccctt
     1861 gcggaaaaat ataatatcag tatcagcgaa agcggacaca aggcgctttt cggcaaaggg
     1921 atgatcggcg gacagaaggt gattctggca aaaccgcaga catttatgaa cttaagcggc
     1981 gagagcgtgg cggaactttt acattattat aagattgatc ccgaggagga gcttctcgtc
     2041 atttttgacg acatcagcct ggcgccgggg aacatccgta tccgcaaaaa agggagcgcc
     2101 ggcgggcata atggaatcaa gaacattatt gcaatgaccg ggacggagaa ctttaagcgg
     2161 attaaggtgg gcgtcggcga aaagccggcg ggatgggatc tggcggatca tgtgctcgga
     2221 cgcatgagtg aggaagaccg cgcggcattt gaggaagcgg tgaaggaatc cgtaaaggct
     2281 gcggagatga tcctggagga tgagattgat caggcaatga atgattttaa caggaaaaag
     2341 cgggagagtc agtcatgaag acattcacac agcccatgca ggggctgaag gaatttgagg
     2401 acctggaggc ggcactgccg aagttatccg gcgttattca gatttccggc tgtatcgatg
     2461 cagccaaacc acatatgatg tacagcattg gcaatggttc tggaaacaga atcattgtta
     2521 cgtttcagga acaaaaagcg aaggaactgg cggaggaata ccgctttttt gatccgaacg
     2581 ttgcctatta tccggcaaag gatattctgt tctaccagtc ggatatccgg ggaaatgtgc
     2641 tgacagcgga gcgtatcaat gccctcaaga tgatggcgga agagcgctcg tgcacgattg
     2701 taacgacgtt tgacggactg atgaatccga tgccgtcgcc ggatcagttt atcagtgcgg
     2761 tgcggaaagt ggcggtcggg gatgtgattg atctggagca gcttacaagg cacctggcgg
     2821 aactgggcta cgagaagaat tatcaggtgc agacaagcgg tgaattctcc gtgcgcggcg
     2881 gaatcattga cattttcccg ctgacggagg agaatccgtt ccggattgaa ctgtgggatg
     2941 atgaggtcga ttcaatccgc tcattcgatg cggaaagcca gcggtcgatc gagaatctgg
     3001 acgaggtcca catctatccg gcgtgcgaac tggtgctgac caaagaggag cgtgacgccg
     3061 gtattgcgcg cattctggcg gaagcaaagc aggtttcgga aaagctccgc ggggagatga
     3121 agaccgagga ggcgtaccgc gtcaagtctg cggcggacca ggtggcggag gaagccgggg
     3181 agctggggct ctctgccggg ctggacgcct acctgtccta tttttgcgag gaacgagtct
     3241 ctttgctcga ttattttgga aaacaggata cgctggtgtt cctggatgaa acaccgcgga
     3301 gcattgagcg aggaatggcg acggagaccg aattttcgga gagcatgaag cagcgcctgg
     3361 aaaaagggta tatactgccg gggcagatgc gggaactttt tacctgcaag gagattctgg
     3421 gaaaacttgc ggggcgcaga tgcatctcgc tggtggcgct cgaccttaaa aatccgcatc
     3481 tggagatcgg aacacgtctg aatatccagt ctaagacagt caatccgtac aacaacagct
     3541 ttgaacttct gataaaggat ctggagcgct acaaaaagaa tggatatcaa gtgattctgc
     3601 tgtcgggatc gagaacgcgc gcgaagcgtc tggcagatga tctgatggcg gaagggctga
     3661 acgcttttta ttcggaagac tacgatcacg aggtaaagcc cggcgagatc atgacgggtt
     3721 acggcaagat caaaaaagga tatgagtatc caatgctgaa gtttgttgtg atctccgaga
     3781 gcgatatttt cggcggcgag aagaagaaaa agaagcgcaa gcgcatctat gagggagaga
     3841 agattgcaag ctttacggat ctgaacatcg gtgactacgt ggtgcatgag agccatggac
     3901 tgggcatcta ccgcggtatt gagaagatcg aggtggataa gaccgagaag gattacatca
     3961 agatcgagta tgcaggcggg ggcaatctct acattctggc gacgcagctg gagcagatcc
     4021 agaaatatgc aggcgcgggg gcgaaaaagc cgaagttaaa caagcttggc ggacaggagt
     4081 ggaacaagac caagtcgagg gtcaagggag cggtcaggga gatcgccgaa gacctcgtga
     4141 agctttacgc agtgcgccag aatgaccggg gatttgcgtt cggaccggac accgtgtggc
     4201 agaaggagtt cgaggagatg ttcccgtttg aggagacgga agaccaggat cttgccatcg
     4261 cagcgaccaa ggccgacatg gagagcacga agattatgga tcgtctgatc tgcggcgacg
     4321 tcggatacgg aaagacggaa attgcgatcc gtgcggcgtt taaggcggtg caggatggca
     4381 agcaggtggc atttctggtg ccgacgacga tcctcgccca gcagcattac aacaactttg
     4441 tgcagcgcat gaaagatttt ccagtcaaca ttgatctgct ctgccggttc agaagtagcg
     4501 cggaacagaa aaagacgatc gaaaagctga aaaaggggca ggtggacatc atcatcggca
     4561 cgcaccggct gctgtccaaa gatgtggtct acaaggatct gggactttta atcatcgacg
     4621 aggagcagcg cttcggcgtg gcgcacaagg agaaaatcaa gcagcttaag accaacatcg
     4681 atgttctcac actgaccgcg acgccgattc cgcggacgct gcacatgagc ctcatcggca
     4741 tccgggatat gagcgtgctg gaagagccgc cgatggaccg tgtgcccatc cagacatatg
     4801 tgatggagta caatgaggaa ctggtgcggg aggcaatctc cagggaactg gcgcgcggcg
     4861 ggcaggcgta ttttgtatac aacagagtgc gcgagatcgc cgatgttgcg nnnnnnnnnn
     4921 atgcgcacgg acagatgaag gagaccgaac tggaaaatat catgtaccgg tttatcaacg
     4981 gcgagatcga tgtgcttgta tccacgacga tcattgagac gggactggat atttcaaacg
     5041 tgaatacgat gattatccac gatgcggaca atatgggtct ttcccagctg tatcagctcc
     5101 gcgggcgtgt gggcagatcg aaccgcacgg cgtatgcgtt tttgatgtat aagcgggata
     5161 agatgttaaa ggaggtcgcg gagaagcggc ttgccgcaat caaagagtac acggagcttg
     5221 gaagtggctt taagattgcc atgcgtgatc tcgaaatccg cggagcgggc agcctgctcg
     5281 gtgagcggca gcacggtcac atggaagcgg ttggttatga tctgtactgc aagatgctaa
     5341 acgaggcggt caaggaagcg aagggcatcg cggtggagga aagctttgac acgtcgatcg
     5401 atattgtgat cgacgcctat atcccgatgg ggtatattcc aaatgaactg cagaagctgg
     5461 acatctacaa acgcatcgca gacattgaga cgcaggagga gacggaggag atgacggagg
     5521 aactgatcga ccgtttcgga gatccgccga agtccgtgga aaatctgctt tacattgcaa
     5581 aaatcaaatc gatggcgcac cggctttatt ttacggaagt ggcgcagaag ggtgagagtg
     5641 tccgttttac tctctatgag aaagcacgga ttgatgtgac gaagattccg gaactggtgg
     5701 cggcatacgg tcagaaggtg acgtttaccg ctgatccgaa gaatccgtac ttcacatatc
     5761 acctgaaaaa gaattcgagg gacaagaatg tggacatcaa atcggtggtg gagcagttcc
     5821 tagccgacgc aggaggattt ctggcggagc gggaagcacc aaaggaaggg taaaaaggtg
     5881 tagaaagcca attttcgggc accggtggac cggctctccg gcgtcggaaa agcttgaaaa
     5941 aagtatgaaa aatattgcga cccggagaaa attgcactat aatagagcaa gactgccatg
     6001 gcaagctgtc atggcatata ccaggaggaa cctatgagag caaagagatg gagcgcactg
     6061 cttcttagcg gtgttatggc agcatccatg ttaacaggat gtggaatgaa taaaaatgag
     6121 acagtggcaa cttttgatga gacggaagtg ccgcttggag tagcaa
//
LOCUS       DBMDQJ010000039         6097 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5223_length_6097_cov_1.236324, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000039 DBMDQJ010000000
VERSION     DBMDQJ010000039.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6097)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6097)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6097
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5223_length_6097_cov_1.236324"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            86..1315
                     /locus_tag="ACZO9R_01415"
     CDS             86..1315
                     /locus_tag="ACZO9R_01415"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR0911764.1"
                     /translation="MKEKVILAYSGGLDTTAIIPWLKENYDYEVICCCIDCGQEEELD
                     GLEERAISCGASKLYIVDIIDEFCDDYIMPCVQANAIYENKYLLGTSMARPLIGKKLV
                     EIARKEGATAICHGATGKGNDQIRFELAIKALAPDLKIIAAWRDDKWTMQSREDEIAY
                     CQAHGIHLPFSADTSYSRDRNIWHISHEGLELEDPACEPNYDHLLVLGVSPEKAPEEG
                     EYVTMTFEKGVPTSVNGKKMKVSDIIRELNRLGGKHGVGIIDIVENRVVGMKSRGVYE
                     TPGGTILMEAHQQLEELVLDRATMEVKKDMGNKLAQIVYEGKWYTPLCDAVRAFVTST
                     QEYVTGEVKFKLYKGNIIKAGTTSPYSLYSESLASFTTGDLYDHHDASGFITLFGLPL
                     KVRAMKMQEIGEKNKYE"
     gene            complement(1428..2852)
                     /gene="pdxR"
                     /locus_tag="ACZO9R_01420"
     CDS             complement(1428..2852)
                     /gene="pdxR"
                     /locus_tag="ACZO9R_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR0911765.1"
                     /translation="MIEIMMNLQGGAKAPLYEKIYEYIKSEITDGRIPRGERLPSTRL
                     LAKNLAVSRSTVEMAYDQLLAEGYIQAEPCRGFFVCDIAELYRLRGSSVGETGQVHRP
                     EKPVCLVDFSPYAIDTRSFPYNVWRKISRNVLLDDSEELLMAGDGQGEYSLRSEIADY
                     LHHARGVNCRPENIVLGAGNEYLEILLTQLLGGNKTVLMENPGYPQAYHTFRNMGYRV
                     VTVPADEQGLSGGTVRAAAPELVYMMPSHQFPTGSVMPLGRRLELLAWAAEQEERYLI
                     EDDHDSEYRYRGKPIPSLQSVDGLGKVIYLGTFSKSIAPSLRISYMVLPQQLIERYRT
                     VCGFYSTTVPRMQQEILREFLRDGHFERHLNKMRGIYRGRHDFLIGELKKRSWVLSVS
                     GDHAGLHVLVEADTGCSEREICERAAAQGVKVSGLGEYALTKDGGGGRNHPVLLLGYG
                     SLTEQEIQMGLTVLDSILDAQESG"
     gene            complement(2872..3726)
                     /locus_tag="ACZO9R_01425"
     CDS             complement(2872..3726)
                     /locus_tag="ACZO9R_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2225 domain-containing protein"
                     /protein_id="HIR0911766.1"
                     /translation="MNLLSGLEKFGLKAEDTTNLFAEEKKEVVGEDGIKQEAVPTEDS
                     FLLDKAVRCAVCDKVFKTKMIKSGRLKRLESDMDLRPRYEHIDTLKYSVISCPYCGYT
                     AITRYFEHLSSMQVKMIREKICVNFKPADNVEPTLIDYDTAIERYKLALFNTIVKKGK
                     NSEKAYTCLNLAWLLRGKRESLDAKDPKMAEQIKECREQEEAFYAQAYEGFTKAVSTE
                     TYPMCGMDECTMDYLLATMAYHYKKYDVASKCIARILQSAAASKKMKDRAYELKERIV
                     EEIKRSKA"
     gene            complement(3757..5445)
                     /locus_tag="ACZO9R_01430"
     CDS             complement(3757..5445)
                     /locus_tag="ACZO9R_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HIR0911767.1"
                     /translation="MENENVSKNFIEQMIDKDIEEGHCKVVHTRFPPEPNGYLHIGHA
                     KSILLNYGLAQKYGGKFNLRFDDTNPTKEKTEFVEAITEDIKWLGADWEGRLFFASDY
                     FDQMYECAVELIKKGKAYVSDLSAEQIREYRGTLTEPGREDPYSSRSIEENLKLFEEM
                     REGKYADGEKVLRARIDMSSSNINMRDPVIYRVAHMTHHRTGDKWCIYPMYDFAHPLE
                     DAIEGITHSICTLEFEDHRPLYEWVINEVGAQIIPDKNEMPPRQIEFAKLYLTNVVTG
                     KRYIKRLVEEGIVDGWDDPRLVSIAALRRRGFTPESLKMFVELCGISKANSSVDYAML
                     EYCIREDLKMKKSRMMAVLDPVKVVIDNYPEGQTEYLDVVNNLENEALGSRKIPFSRE
                     LYIEREDFMEEPPKKYFRMFPGNEVRLMNAYFVTCNSFEKDADGNVTVIHCTYDPASR
                     GGNSPDGRKVKGTIHWVSATHAVPATVRLYENIVDEEKGVYNEDGSLNLNPNSLTVLK
                     NCMVEENLAGAKAYDSFQFVRQGFFCVDAKDSTPEQLVFNRIVSLKSSFKLPTA"
     gene            complement(5610..>6097)
                     /locus_tag="ACZO9R_01435"
     CDS             complement(5610..>6097)
                     /locus_tag="ACZO9R_01435"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741323.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR0911768.1"
                     /translation="KMSKSLGNCIYLSESEADVKKKIMSMFTDPDHIRIEDPGKLEGN
                     TVFTYLDAFCTDGHFAEYLPEYANLDELKAHYKRGGLGDVKVKKFLNNVMQEELSPIR
                     ARRKEYEQRIGDVYDILKAGSEVAKEEAAKTLSEVKHAMKIDYFDTPEFLEEQVKKYS
                     E"
BASE COUNT         1415 a         1780 c         1329 g         1573 t
ORIGIN      
        1 ttatgtatat tttttcactt gcttttccgt tgcatatgtt gtatattgat tgcagttaat
       61 gatttacaga aaggaaaaaa agcccatgaa agaaaaagtt atcttagctt actccggcgg
      121 tctggacacc accgccatca ttccctggtt aaaggagaat tacgattacg aagtcatctg
      181 ctgctgcatc gactgcggtc aggaggaaga actggacggt ctggaagaaa gagccatctc
      241 ctgcggagct tccaagcttt acatcgtcga catcatcgac gagttctgcg atgactacat
      301 catgccgtgc gtacaggcaa acgccatcta cgagaacaaa tatctgctcg gcacttccat
      361 ggcacgtccg ttaatcggca agaagctggt tgagatcgca agaaaagaag gcgctaccgc
      421 catctgccac ggcgctaccg gaaaagggaa cgaccagatc cgttttgagc ttgccatcaa
      481 ggcactcgct ccggatctca agattatcgc tgcatggaga gacgacaaat ggaccatgca
      541 gtcccgcgag gatgagatcg catactgcca ggcacacggc attcatcttc cgttctccgc
      601 agacaccagt tacagccgcg accgcaacat ctggcatatc agccacgagg gtcttgagct
      661 cgaggatcct gcctgtgagc cgaactacga tcacctgctg gtactcggcg tctccccgga
      721 aaaagctcct gaggagggcg agtatgtgac catgaccttc gagaaaggtg ttccgacctc
      781 cgtcaacggc aagaagatga aggtttccga tattatccgt gagttgaacc gtctcggcgg
      841 caagcacggc gtcggcatca tcgacatcgt ggagaaccgt gttgtcggca tgaagtcccg
      901 cggtgtctat gagactcctg gcggaaccat tctgatggaa gcacatcagc agctcgagga
      961 gctggtactt gaccgtgcta ccatggaagt gaaaaaggat atgggcaaca agctcgctca
     1021 gatcgtatac gagggcaaat ggtacacccc tctctgcgac gcagtgagag catttgtcac
     1081 ctccacacag gaatatgtga ccggtgaagt caagttcaag ctttacaagg gcaacatcat
     1141 caaagccgga acgacctccc cgtattctct ctacagcgag tccttggcaa gcttcaccac
     1201 cggcgatctc tacgatcacc acgatgcttc cggattcatc acactgttcg gactgccgtt
     1261 aaaagttcgt gcaatgaaga tgcaggaaat cggcgagaaa aacaaatacg aataaatcgt
     1321 acatccggat tgcttacaca agaacccccg gaaatctgcc gtttcggcag atttccgggg
     1381 gttccttttc tgtccggtta ttcacattcc aatcattttc tttctgatca tccgctttcc
     1441 tgcgcatcca gtatcgaatc cagcacggta agacccatct gaatctcctg ctcggtcagg
     1501 ctgccgtatc caagcagcag caccggatgg tttctgccac cgccgccatc tttcgtcagt
     1561 gcatattcgc caagacccga tactttcacg ccctgcgccg ccgcacgttc acagatctcc
     1621 cgctcactgc atccggtatc cgcctccacg agcacatgga gtcccgcatg gtctccactg
     1681 actgagagca cccagcttct ttttttcagt tcaccgatca aaaaatcatg cctgccgcgg
     1741 taaatgccgc gcatcttgtt gagatgccgc tcaaaatgcc catcccgcaa gaactcccgg
     1801 agaatctcct gctgcatgcg cggcaccgtc gtcgaataaa atccacagac ggtgcggtag
     1861 cgctctatca gctgctgcgg aagcaccata taactgatac gcagcgacgg cgcgatactt
     1921 ttggaaaatg ttccaagata aattaccttc ccgagtccat ccacgctctg cagcgacgga
     1981 atcggctttc cacggtaacg gtactcgctg tcgtgatcgt cctcgatcag atagcgctcc
     2041 tcctgctccg ccgcccacgc aagaagttcc agtcttctgc ccagaggcat gacgctgccg
     2101 gtcgggaact gatgggacgg catcatgtag acaagctccg gtgccgccgc acgcaccgta
     2161 ccgccgctaa gtccctgctc gtccgccggc accgtcacca cccggtatcc catattccgg
     2221 aaggtgtggt atgcctgcgg atatcccggg ttctccatga gcacggtctt atttcctccg
     2281 agaagctgcg tgagcagaat ctccagatac tcatttccgg ctccgagcac gatgttttcc
     2341 gggcggcagt tcacacctct ggcgtgatgc agataatccg caatctcact gcgcagactg
     2401 tactccccct gtccgtcccc cgccattaac agttcctcac tgtcgtccaa cagcacattc
     2461 cggctgatct tccgccagac gttgtacgga aaactgcggg tatcgatcgc atacggggaa
     2521 aaatcaacca ggcagaccgg tttttccggt ctgtgtacct gtcccgtttc tccgacgctg
     2581 cttccccgaa gccggtacag ctccgcgata tcgcatacaa aaaaaccccg gcagggttcc
     2641 gcttgtatat atccctctgc caggagctga tcatatgcca tctctacggt gcttctgctc
     2701 accgcaagat tcttcgccag aagacgcgtg gacggcaacc gttcccctct cggaatcctg
     2761 ccatctgtaa tctcgctctt gatatattcg tatattttct catagagggg agctttcgct
     2821 cccccctgta agttcatcat aatctctatc atcgtaaagc cttatccgcc gttatgcctt
     2881 gcttctcttg atctcctcga caatgcgctc cttgagttcg tacgcgcggt ccttcatctt
     2941 cttggatgcc gccgcagact gcaggattct tgcaatgcac ttcgatgcaa catcgtactt
     3001 tttgtagtgg tatgccatcg ttgcgagcag ataatccatc gtgcactcat ccatgccgca
     3061 catcggatag gtctctgtgg ataccgcctt ggtaaagcct tcatacgcct gtgcatagaa
     3121 cgcctcttcc tgttccctgc actccttgat ctgctctgcc atcttcggat ccttcgcatc
     3181 taaagactcg cgcttgccgc gcagcagcca tgcaagattc aggcaggtgt acgccttctc
     3241 actgttctta ccttttttca caatcgtgtt gaacagtgcc agcttgtagc gctcgatcgc
     3301 agtatcataa tcgatcagcg tcggctccac attgtccgcc ggcttgaaat tcacacagat
     3361 cttctccctg atcatcttga cctgcatgga ggacaggtgt tcaaaatagc gcgtaatcgc
     3421 ggtatagccg cagtacgggc aggaaatcac actgtacttt aaagtgtcga tgtgctcata
     3481 gcggggacgc agatccatgt cggactcaag acgctttaat cttccgctct tgatcatctt
     3541 tgtcttaaat accttgtcgc acactgcaca gcgcacagcc ttgtccagaa gaaagctgtc
     3601 ttctgttggc accgcctcct gctttatgcc gtcctctccc acgacttctt tcttttcttc
     3661 cgcaaaaaga ttcgtggtat cctctgcctt taaaccaaat ttttccaaac cggataataa
     3721 attcatatct attcctccta aatctcaggc cgcgtcctac gcagttggca gcttgaatga
     3781 actcttcagt gacacaatgc ggttgaacac cagctgctcc ggcgtcgaat cctttgcatc
     3841 cacacagaag aatccctggc gtacaaactg gaaactgtcg taagcctttg ccccagcaag
     3901 attctcctcc accatacagt tctttaacac agtcagtgaa ttcggattga ggttcagact
     3961 tccatcctca ttgtataccc ctttttcctc gtccacgata ttctcataca gtctgaccgt
     4021 tgcaggaacc gcatgtgttg cggataccca gtgaatcgta ccttttacct ttctgccgtc
     4081 cgggctgttg ccgccgcggg acgccggatc gtaagtacaa tggattaccg tcacgttacc
     4141 gtctgcatcc ttctcaaagc tgttgcaggt tacaaaatat gcattcatca ggcgcacttc
     4201 attgcccggg aacatacgga aatacttctt cggcggctcc tccatgaagt cttcacgctc
     4261 gatatagagt tctcttgaga acggaatctt acgtgagcca agcgcctcat tctccagatt
     4321 gttcacaacg tccagatatt cggtctgtcc ttccggatag ttgtcaatca caaccttcac
     4381 cggatcaaga actgccatca tacgggactt cttcatcttg agatcctcgc ggatgcagta
     4441 ctctaacatt gcatagtcaa cagaactgtt tgcctttgaa ataccgcaca gttctacgaa
     4501 catctttaat gattccggtg tgaatcctct tctgcgcagc gctgcgatgg ataccagacg
     4561 tggatcatcc catccgtcca caataccttc ctccaccagg cgcttgatgt aacgcttgcc
     4621 ggttaccacg ttggtcagat acagctttgc aaactcaatc tgtctcggcg gcatctcatt
     4681 cttatccggg ataatctgtg cccccacctc gttgatgacc cactcataga gcggtctgtg
     4741 atcctcaaac tccagcgtac agatggaatg cgtgattccc tcgatcgcat cctcaagcgg
     4801 atgtgcaaaa tcgtacatcg ggtagatgca ccatttatcg ccggttctgt ggtgtgtcat
     4861 gtgtgccaca cggtaaatga caggatccct catattgatg ttggaagagc tcatatcaat
     4921 gcgtgcacgg agcactttct caccgtccgc atactttcct tcccgcatct cctcaaacaa
     4981 ctttaaattc tcctcgatgc ttctgctgct gtacggatct tccctgcccg gctccgtcag
     5041 cgttccacgg tactcacgga tctgctctgc agacagatcg gagacataag cttttccttt
     5101 tttaatcagc tctacggcac actcatacat ctgatcgaaa tagtcggatg cgaagaaaag
     5161 tctgccctcc cagtccgcac caagccactt gatgtcctcc gtgatcgcct ccacgaactc
     5221 ggtcttctcc ttggtaggat ttgtatcgtc aaatctcaga ttaaactttc cgccatactt
     5281 ctgtgccaga ccataattca aaagaatgga cttcgcatgg ccgatatgca gatagccatt
     5341 cggttccggt ggaaatctgg tatgaacaac cttgcagtgc ccttcctcga tgtccttgtc
     5401 aatcatctgc tcaataaaat tctttgagac attttcattt tccataatgt cgcatcctcc
     5461 tcaattacaa tccggacagc ctgccgtccg gtctgctttc cctgtcaaaa aaagagaaag
     5521 caactcatga aatatcatac atgatacacc gtcaaggtgc aagtataata taacatgaat
     5581 tgcctgaatt tgtaaaaaac aatttgtgat tactcggaat acttcttcac ctgttcctcc
     5641 aggaactccg gtgtatcaaa ataatcaatc ttcatggcat gctttacctc ggaaagtgtc
     5701 tttgcagcct cttcctttgc cacttcgctt cccgccttta aaatatcata cacatcgccg
     5761 atgcgctgct catattcctt cctgcgcgca cggatcggag acagctcctc ctgcatgaca
     5821 ttattcagga acttcttcac cttgacatcc cccagaccgc cgcgtttgta atgtgccttc
     5881 agctcatcca ggttcgcata ctccggcaga tattccgcaa aatgcccgtc cgtacagaat
     5941 gcatccagat aggtaaatac agtatttcct tcgagttttc caggatcttc gatccggata
     6001 tgatccgggt ccgtgaacat ggacatgatc ttcttcttca catccgcctc gctctccgag
     6061 agatagatgc agttgccgag cgacttgctc attttgg
//
LOCUS       DBMDQJ010000040         6085 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5236_length_6085_cov_1.352273, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000040 DBMDQJ010000000
VERSION     DBMDQJ010000040.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5236_length_6085_cov_1.352273"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(216..821)
                     /gene="rsxE"
                     /locus_tag="ACZO9R_01440"
     CDS             complement(216..821)
                     /gene="rsxE"
                     /locus_tag="ACZO9R_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HIR0911769.1"
                     /translation="MNKNVERLYNGLIKENPTFVLMLGMCPTLAVTSSAINGLGMGLS
                     TTVVLVLSNMLISAFRKVIPDGVRMPAYIVIVASLVTVVQFIMQAYTPSLSESLGVYI
                     PLIVVNCIILGRAESYASKNPVVPSLFDGLGMGLGFTMGLTLIGLFRELLGAGQIFGF
                     QILSLSWFTPITIFLIWTHPFKAFPVWQESISRSVLLIGIC"
     gene            complement(825..1433)
                     /locus_tag="ACZO9R_01445"
     CDS             complement(825..1433)
                     /locus_tag="ACZO9R_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HIR0911770.1"
                     /translation="MNKKIVHDALVLFAFTVVLGLILGVVYGITKGPIDQVNYEKTQS
                     AYKQVFEDADSFEEYADFDTAAAEDLMAQNGYSDDIEAVNTALDASGNPLGYVLTVTA
                     KDGSQGSITFSVGIQNDGTVNGYSITSISETPGLGMKAQEEPFYSQFEGKNVDSFTVV
                     KSTPSADNEIEAISGSTITSKAMANGVNACLTYFHSVLEGGN"
     gene            complement(1436..2401)
                     /locus_tag="ACZO9R_01450"
     CDS             complement(1436..2401)
                     /locus_tag="ACZO9R_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HIR0911771.1"
                     /translation="MSDLYHVSSSPHVRAKDTTSRIMLYVIIALLPTSLFGIYNFGYK
                     ALVLILVTIASCVASEWVFNKIVHKKQTIDDLSAVVTGLLLALNLPATLPWWEAVIGG
                     VFAIVVVKCLFGGLGQNFMNPALGARCFLLIAFAADMTKFGITKNGIEVYSSVTPLAA
                     MRNGEAVNTMDMLIGRTAGTIGETSAIAILIGAIFLILMGVINLRIPASYIITFAVFM
                     LLFGGHGFDGSYLTAQLCGGGLMLGAFFMATDYVTSPITPMGQIIFGICCGILTGLFR
                     CFGANAEGVSFAIILSNILVPLIEKHTVPMAFGQVKEAKKQEGGK"
     gene            complement(2434..3753)
                     /gene="rsxC"
                     /locus_tag="ACZO9R_01455"
     CDS             complement(2434..3753)
                     /gene="rsxC"
                     /locus_tag="ACZO9R_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HIR0911772.1"
                     /translation="MGSKTFKGGVHPYEGKELAKDQPIVEVLPKGDLVYPLSQHIGAP
                     ATPVVAVGDKVLKGQKIAEAGGFVSSPIYASASGTVKAIEPHRVAVGDMVNSIVIEND
                     GAFEEVEYTPCEDVTALSKEEIVNKVKEAGVVGMGGAGFPTHVKLSPKEPEKIEYVIA
                     NCAECEPYLTADYRRMLENPEELIGGMKIILQLFDHAKGVLGIEDNKPDCIEKLTELV
                     KDEPRIEVCPLQTKYPQGGERQLIYAVTGRAINSKMLPADAGCIVDNVETIIAVYRAV
                     KLGRPVTNRISTITGDAIANPGNFLYSIGTSYAELVEAAGGFKTQPEKIISGGPMMGF
                     AMFSLDIPTTKTSSSLLCLSKDEVSKVEPSACINCGRCVEACPEQLIPSRLAKFSNNG
                     LAETFESWHGLECVECGSCSFVCPARRQLAQSIKTMKKQVLAAKRKK"
     gene            4169..5278
                     /locus_tag="ACZO9R_01460"
     CDS             4169..5278
                     /locus_tag="ACZO9R_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HIR0911773.1"
                     /translation="MITWHEELTFDFSDPVSNEVFTENCIFFDIETTGFSPARTDLYL
                     IGCATRRDSLLCIDQFFSENAADQKNVLSAFLELLSGYDTILTFNGIGFDIPYLKAKC
                     KALHLSDPFDAHSYIDLYKEVSRFKFLFALTSYKQKSIELFLGIDRIDAYSGGELIEV
                     YKEYVRHPAKDSLALLKQHNYEDVLYMPKLLPVLSYPKLWEQAFSLQSLQASEYRSMD
                     GASGNKELFFTLALQYPVPKPVSFSYDDCYLSMSGSTARLRVRLFEGELRFFYDGSPK
                     DYYYLPAEDIAVHKSIASAVDKEHRVQANASNCYGKKYAIFLPQYDAMFSPVFREQPR
                     GRKCYFELSADFAADPAMQMDYVLHLLETMRSHRK"
     gene            complement(5404..>6085)
                     /locus_tag="ACZO9R_01465"
     CDS             complement(5404..>6085)
                     /locus_tag="ACZO9R_01465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HIR0911774.1"
                     /translation="NTLITKNHIATYDEYVALIKKDKEKFEQFINFLTINVSEFYRNP
                     DQWKILENEVFPKLIQKFGKNLKIWSAACSTGDEPYSLVMALSRHLPLANIKVIATDI
                     DKQVIDTARMGLYNEKSIASVPDDLKKKYFTKVGNSYQISDEIKKRVEFKEHDLLKDP
                     YPGGCNLIVCRNVVIYFTEEAKDEIYAKFYKALATGGVLFIGSTEQIMNYREIGFERD
                     KSFFFEKA"
BASE COUNT         1562 a         1645 c         1308 g         1570 t
ORIGIN      
        1 gtttcatcct atattttagg aaataaccgg ataaaccatg atccattttg ctattatatt
       61 ccaagtgtcg tactgcataa acgccgctgc caataccggt ccaaatatga gcactgccaa
      121 aatcagggct ccgacctttt tgttttgtat aaaatataga atactatagg tcacgactgc
      181 aaagatcatt gtatttacta ctcgaagtaa cagcgtcaac aaataccaat gagcaatact
      241 gatcgtgata tgctttcctg ccaaacagga aatgctttga atgggtgcgt ccaaataaga
      301 aagatggtga tcggtgtgaa ccagcttaag gacagaatct ggaaaccaaa gatctgacct
      361 gcgcccagaa gctcacggaa cagtccgatc agggtaagac ccatcgtaaa tccaagtccc
      421 ataccaagtc catcgaaaag agacggaact accggattct tggaagcata gctctcagca
      481 cggccgagga taatacagtt tacaacgatt aacgggatgt acacacccag cgactcggag
      541 aggctcggag tatacgcctg catgataaac tgcaccactg ttaccagcga tgcaacgata
      601 acaatgtatg ccggcatacg cacgccgtcc ggaatcacct tacggaatgc ggaaatcagc
      661 atattggaaa gtaccagtac cactgttgtg gaaagtccca tgccaagtcc gttgattgcg
      721 gaggatgtga cagcaagtgt cggacacata ccaagcatca gaacgaaagt aggattttcc
      781 ttaatgagtc cattatataa acgttctaca ttcttgttca ttgattagtt acctccttct
      841 aatacgctgt ggaagtatgt aaggcaggca ttcacaccgt tcgccatcgc cttggaagta
      901 atggtggaac cggaaattgc ctcgatctca ttgtccgcag acggtgtcga cttcacaacc
      961 gtaaaggagt ctacgttctt gccctcaaac tggctgtaga acggctcttc ctgtgctttc
     1021 ataccaagtc ccggtgtctc ggaaatactt gtgatggaat aaccgttgac cgtaccgtca
     1081 ttctggatgc cgacagagaa tgtaatgctg ccctggcttc cgtccttcgc cgttacggta
     1141 agcacatagc cgagcggatt gccggaagcg tcaagtgctg tgttgacagc ctcgatatcg
     1201 tcagaatatc cattctgcgc cataagatct tccgctgcag cggtgtcgaa atcagcgtac
     1261 tcctcaaagg aatccgcatc ctcaaatacc tgcttgtaag cggactgtgt cttctcataa
     1321 tttacctggt cgatcgggcc tttggtaatg ccgtagacca cgccgaggat cagacctaat
     1381 acaacagtaa aagcaaataa aaccaatgcg tcatgtacaa tcttcttgtt catttctatt
     1441 tgcctccttc ctgcttcttc gcttccttca cctgaccgaa tgccatcggc accgtgtgct
     1501 tctcaataag cggcacaagg atattgctta agatgatggc gaaggaaaca ccctctgcat
     1561 tcgcgccgaa gcagcggaac aatccggtca ggataccgca gcagatacca aagatgatct
     1621 gccccatcgg tgtaatcggg gatgtcacat aatccgttgc catgaagaat gcgccgagca
     1681 tcagaccgcc gccgcaaagc tgtgcggtaa gatagctgcc gtcaaatcca tgaccgccga
     1741 ataaaagcat gaatacagca aatgtaataa tgtaagatgc cgggatgcga aggttgatca
     1801 cacccatcag gatcaggaaa atagctccga tcagaatagc gatcgcagat gtctcaccaa
     1861 tggtacctgc ggtgcgtccg atcagcatat ccatcgtatt gaccgcctca ccgttacgca
     1921 tggcagcaag cggtgttacg ctggaataca cttcaatacc gttctttgtg atgccaaact
     1981 ttgtcatatc agcagcaaat gcaatcaaaa ggaagcatct tgcgcccagc gccgggttca
     2041 tgaaattctg tcccagtccg ccaaacagac attttacaac cacgattgca aacacaccgc
     2101 cgattaccgc ctcccaccac ggaagggttg ccggaaggtt caatgccaga agcagtcccg
     2161 ttacaacggc gctcaggtca tcgatcgtct gctttttatg tacaatctta ttaaataccc
     2221 actctgatgc cacacagctt gcaatcgtta ccagaatcag aaccaatgcc ttatatccga
     2281 aattgtaaat tccgaacagg cttgtcggaa gcaatgcaat aatcacatac agcataatac
     2341 ggcttgtggt atctttggca cgaacgtgtg gtgatgatga aacatgatat aaatcactca
     2401 cgataatcca cctctctctt cttgtttttt gaattatttt ttgcgttttg cagcaagtac
     2461 ctgctttttc atcgtcttaa tggactgtgc gagctgtctg cgtgcagggc atacaaaact
     2521 gcagcttccg cactccacgc actcgagtcc gtgccaggat tcaaacgtct cagccagacc
     2581 gttattggaa aacttggcaa gtcttgacgg aatcagctgc tccgggcatg cttccacaca
     2641 gcggccgcag ttgatacatg cggacggctc caccttggac acttcgtcct tgcttaagca
     2701 aagcagtgag gaagatgtct ttgtcgtggg aatatccagg ctaaacattg caaatcccat
     2761 catcggacca ccggaaataa tcttctccgg ctgtgtctta aatccgccgg cagcctccac
     2821 cagttctgca tagctggtac cgatgctgta taagaagttg cccgggttgg caatggcatc
     2881 gcctgtaatc gtagaaatac gattggttac cggtctgcca agctttaccg cacggtaaac
     2941 agcaatgatg gtctccacat tatccacgat acatcctgca tctgccggaa gcatcttgga
     3001 attgatggcg cggcctgtca ctgcataaat gagctgacgc tcaccaccct gcgggtactt
     3061 ggtctgtaaa ggacagacct caattctcgg ttcatccttg acaagctctg tcagcttttc
     3121 aatacaatcc ggcttgttgt cctcgatgcc gagaacaccc ttcgcatgat cgaacagctg
     3181 gagaatgatc ttcattccac cgatcagctc ttctggattt tcgagcattc tgcggtagtc
     3241 cgccgtcaga tacggctcac actctgcgca gtttgcaatt acatattcaa tcttttccgg
     3301 ctctttcgga gacagcttca catgtgtcgg gaaacctgca ccgcccatgc cgacaacgcc
     3361 tgcctctttt accttattga caatctcctc cttggagaga gccgtcacgt cttcacacgg
     3421 cgtgtactcc acctcttcaa acgccccgtc gttctcaatg acgatggaat tcaccatgtc
     3481 accgactgcc acacggtgcg gttcgattgc ctttactgta ccggatgccg acgcatagat
     3541 cggggatgac acaaagccac ctgcctctgc gatcttctgt cctttcagca ccttgtcacc
     3601 aaccgctacc accggagtcg ccggtgcacc gatatgctgt gacaacgggt ataccagatc
     3661 acccttaggt agaacttcta cgatcggctg atcctttgcc agttcctttc cttcataagg
     3721 atggacgccg cctttaaatg tcttagaacc catttctcgt gccccacttt ctcttatcta
     3781 tattatattc tatgccaaaa gtcatagaat accctcgtgt agattatagc acaatcactc
     3841 ggaaattgta gcaatttatt gtattttttc acgaacctat tgacaaagaa ttcacaatat
     3901 tttttccaca tttatgttat aatgtacgtg aaggcaaaag tgagcatgga gcaaaaacat
     3961 acgctcatgc ttgcatgcag cggatggttc tgctccatgt gaacgcgccc gcgagtccgg
     4021 gaagccgcag gctttccgga cttttgccgg agcgtacatt gctatttgct gcgaacgccc
     4081 gcgagtccgg gaaatctatg cctttccaga cctgcacagc ggcaaaattg gaactcttac
     4141 cctaatatta catacagagg attttcagat gataacctgg catgaagaac tgacttttga
     4201 tttttcagac cccgtttcca acgaggtatt tacagaaaac tgcatttttt ttgatattga
     4261 gacaaccggc ttctcgccgg cgcgcacgga tctctacctg atcggctgcg ccacaaggcg
     4321 cgacagtctt ttgtgcatcg accagttctt ttccgagaac gccgccgatc agaaaaatgt
     4381 cctttccgcc tttctggagc tcttgtccgg ctatgatacc attctgacgt tcaacggtat
     4441 cggcttcgac attccatacc tgaaggcgaa atgcaaggcg ctgcaccttt ccgatccgtt
     4501 tgatgcgcac agttatatcg atctttataa ggaagtctca cgtttcaaat ttctgtttgc
     4561 cctgaccagc tacaagcaga aatccatcga actgtttctc gggattgacc gcatcgacgc
     4621 ctactccggc ggcgagctga tcgaagtcta caaagagtac gtgcggcatc ccgcaaagga
     4681 ttcccttgca ctgctgaaac agcacaacta tgaggacgtt ctctatatgc cgaagcttct
     4741 gcccgtcctc tcctacccaa aactctggga acaggcattt tctctgcagt cactccaggc
     4801 gtccgagtac cggtcgatgg acggtgcctc cggcaacaag gagctgtttt ttacactggc
     4861 gctgcagtac cccgttccca aaccggtctc cttctcctac gatgactgtt atctctccat
     4921 gagcggaagt accgcccggc tgcgtgtacg tctcttcgag ggagaactgc gcttctttta
     4981 tgacggcagt cccaaagatt actactatct ccctgcggag gatatcgcag ttcacaagag
     5041 cattgcatcc gccgtcgaca aagaacaccg cgtgcaggcg aacgcttcca actgctacgg
     5101 caaaaaatat gccatctttc ttccgcagta tgacgccatg ttttccccgg tgttccgtga
     5161 acagccgcgc ggcaggaaat gctattttga attatccgcg gactttgccg ccgatcccgc
     5221 tatgcagatg gactatgttc tgcatctgtt agaaaccatg cgttcccacc gcaaataaac
     5281 acatggcaga aagccctgat ataattacca ccaaaaacgg caggaaagaa tctggcatat
     5341 actgcacagc ttctttcctg ccgctttttt gctttataga aaaatgcgtc ctctgatata
     5401 accttacgcc ttctcaaaga aaaaggactt gtctctctca aatccgatct cccggtaatt
     5461 catgatctgc tccgtgcttc cgatgaaaag cacgccgcct gttgcaagcg ccttataaaa
     5521 cttcgcataa atctcgtcct ttgcctcctc ggtaaaatag atcacaacgt tccggcacac
     5581 aatcagattg catccgcctg gatacggatc ttttaaaaga tcatgctcct tgaattccac
     5641 acgtttcttg atctcatccg agatctgata cgaattgccg accttggtaa aatacttctt
     5701 cttcaaatca tccggcacac tcgcgatact cttctcattg tagagtccca tccgcgccgt
     5761 atcgatcacc tgcttgtcaa tgtcggtcgc aatcaccttg atgtttgcaa gcggcaggtg
     5821 tctggacaat gccattacca gcgaatacgg ctcatctccg gtcgagcagg cagcactcca
     5881 gattttcaga ttcttgccaa acttctgaat cagcttcggg aacacctcat tctccagaat
     5941 cttccactga tccggattgc ggtaaaactc cgacacattg atggtcagga aattgataaa
     6001 ctgttcaaac ttctctttgt ccttcttgat cagcgccaca tactcatcat atgtcgcaat
     6061 atgattcttc gtaatcagcg tgttg
//
LOCUS       DBMDQJ010000041         6046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5277_length_6046_cov_1.328343, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000041 DBMDQJ010000000
VERSION     DBMDQJ010000041.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5277_length_6046_cov_1.328343"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="ACZO9R_01470"
     CDS             complement(<1..474)
                     /locus_tag="ACZO9R_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR0911775.1"
                     /translation="MTNEQIRDFLEKHAEKQFKDFTSSLIPGTDSILGVRLPVLRLLA
                     KQLAKEEWRTYLKEARDDSYEEIMLQGLVIGYVKAEMEELLEYAAAFIPKIHDWSVND
                     GFCSTFKIVRKHRAEVWDFLMQYCASKSEFEQRVVAVMLMDHFLVPEYIDRVLAVY"
     gene            complement(1318..2055)
                     /locus_tag="ACZO9R_01475"
     CDS             complement(1318..2055)
                     /locus_tag="ACZO9R_01475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911776.1"
                     /translation="MDAVMDMEPAICAATKAESIENTDVITMRNINKSYRMGTQSLHV
                     LKDVSLTVKQGEYLAILGPSGSGKSTLMNIIGCMDVMDSGNYLLDGVAIEQAKEKELV
                     KIRNQKIGFIFQKYHLIPTYNVLQNIVMPLLIRGMGLAEAQEASMDTIDMLGLSQRIG
                     HKPNELSGGQQQRVAIARALVGKPAILLADEPTGALDVNSGKEVLKLFENLNQMGNTI
                     VMITHDLNVAKHAGRVVRIVDGEIYEN"
     gene            complement(2043..>2274)
                     /locus_tag="ACZO9R_01480"
     CDS             complement(2043..>2274)
                     /locus_tag="ACZO9R_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911777.1"
                     /translation="GDATSDGTGSNGGGAPGGNGGMSGKSVTTLIPVSTVVHTTAGTE
                     TTFQRLAAGDLLKLLIVTSEDGEEVIVEIWMQ"
     assembly_gap    2275..2290
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2291..2762)
                     /locus_tag="ACZO9R_01485"
     CDS             complement(<2291..2762)
                     /locus_tag="ACZO9R_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911778.1"
                     /translation="MIRKKVMAGGIVALAAVLGGGGIFAWTHRTADMETLADNQKYVY
                     AYVTSIEGNELTYMEVDESVVEAYLASSTEDTENAEKGGDGSGVPGGSDGSGAPGGSD
                     TAGAPEDGSGAPGGSDGSGAPGDGSGAPGRMSQGTSGTNDSTVMSYGMPQEENGD"
     gene            complement(2784..4223)
                     /locus_tag="ACZO9R_01490"
     CDS             complement(2784..4223)
                     /locus_tag="ACZO9R_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0911779.1"
                     /translation="MKPKELLRLVWINITQNKFKVVMTSIGIIVGAATIVMVIAIGKG
                     GQADVADQFKNLNAGAVDISYDGGTDFGGGGSSDDSDSGMAMMAPGGGSGGGFGGGFG
                     GAPSGDMGGGSSSGGEAPGGSPGGEMPSGDGGGMPDMGSFFGGQSDMEDRMNQENITL
                     SEDDVDDIETFASGISAATISYTTKSSVDGGDLDEADTYTIAGVKSNYETISNLSLAV
                     GSFITDDDDENKEKVCVLGASVAEEIFGSAIEAYGSVIYIDDRLYVVNGVLESMGTVA
                     SGISPDDSIFIPYATGVKYLVGSSVSPTITVLAADVNNVEGVIEEVTEVLATSYPNAE
                     FTFEDAGSKMEAAEASNQILTMLLIAMAAIVFVVGGIGIMNVLFVSVKERTNEIGILK
                     AIGCSKRDILLEFLAEAAAISLIGGVLGNLASLAVAPIVQYLGVRVELSASAFLIALV
                     FAVGTGTIFGFYPAYKASNLVPVEALGAE"
     gene            complement(4220..5674)
                     /locus_tag="ACZO9R_01495"
     CDS             complement(4220..5674)
                     /locus_tag="ACZO9R_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR0911780.1"
                     /translation="MKKKKLWLVTGVAAAAVLAAGTVTFAYQSGSDSEEQTVYKEVTV
                     EKGNLTAGVTESGSVTIGTLDQTLDFSEDSSSASTQNNSSAQNSAGENATSSTSTAST
                     LEVETVYVTAGQSVAEGDPILKLTEESVASYKKDLEEAVTEAKTAVSEAGLSAEKQKV
                     SASYSYNLSTAEGSVAQETYEAAIKQLQEAVDDAQEAVDTSASLINYYQEQIDAGVDL
                     SASLTEEQENYDKLYTKLVAAKNNYTTKSIEAEKTLKEAELSAKNASSQYSVDVSGAD
                     NDITDAKDTLSDAEEALEAFEAAIGDDGTIYAEYTGIITNLSYEAGDTISSGATVATF
                     SNTDAVTITVSVSEEDITNIAVGDVVEIELSAYEDQTFAGEVESIDTSSSSGSSTVSY
                     NVTAAFTGDITGIYTDMTGNVTFISRQVTDVLYVSNKAVQSDGTTSYVKVKDADGTIR
                     MVDVETGFSDGVNVEITSGLTEGETVLIESQVTE"
     gene            complement(5671..>6046)
                     /locus_tag="ACZO9R_01500"
     CDS             complement(5671..>6046)
                     /locus_tag="ACZO9R_01500"
                     /inference="COORDINATES: protein motif:HMM:NF024829.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR0911781.1"
                     /translation="TAFASYYDLDTILISVEVSQNNIAKLAVGDTVQVQISGSRMGAI
                     DGTVYSIASEKTSGGSMSNVTYAVIVAVDNTDGTLTTGVQATVKFETQATGATEMEET
                     KTAGKTETIGASETEEAGGKTE"
BASE COUNT         1342 a         1844 c         1239 g         1605 t
ORIGIN      
        1 atatacagcg agcacacggt caatatattc aggcaccaga aaatggtcca tcagcatcac
       61 tgccaccacc cgctgttcaa attcactctt agacgcgcag tattgcatca aaaaatccca
      121 cacctctgcc cggtgctttc ggacaatctt aaatgtagaa caaaacccat cattgactga
      181 ccagtcgtgg attttcggaa taaacgctgc ggcatattcc aaaagctcct ccatctccgc
      241 tttcacgtag ccaatcacaa gtccctgcag catgatctcc tcgtagctgt catcgcgtgc
      301 ctctttaaga taagttctcc attcttcctt tgcgagctgc ttcgccagca agcgcagcac
      361 cggaagccgc actcccagaa tcgaatctgt ccccggaatc aggctgctgg taaaatcttt
      421 gaactgcttt tccgcatgtt tctctaaaaa atcacgtatc tgttcgtttg tcattctttc
      481 ctctctttgc actgtcaatg aattactgta aatgcgattc tgtgtctgca ctgttttctg
      541 cgcacaatat gaatctgtca agattcatta tatcatcttt ttgcgctgct ttctgcgcat
      601 ccgaagttcc gcaggaactt ctaatatgaa tccgatagga ttcattatat catacatcaa
      661 cgataccccg gcaaaaggaa ttatcgcaat aagcctagac ctcttctggg tgatgtgtaa
      721 tattattaca aacacacttg gggatttcta ataatattac ttatcaaagc agggcgcaat
      781 atgcttattg cggcaattcc atgcaagcgc gttcgcatgg aacatatccg tccgctgcac
      841 gtaagcatgc gcatccgtct ttgcttcatg ctcacttttg tctccgcgaa cattatgtta
      901 caaaccgtgg attcgggtct atgcacgcaa aatcgtgctc tcagacccgc aaaatgacag
      961 aatagttgac ataacaacag tataaaagag tagaacgggt ctcagacgca aaaaagcagc
     1021 tctcagaccc gcgaaatagt caagaacggg tctcagatgg caaaatgtat ctcccagacc
     1081 cgcaaaacag cagaatggtc gacataacac caatgtaaaa tggtaaaaca ggtcccagat
     1141 gccgaaaaat gtctctcgga cccgcgaaat agtcaaaaac ctgtctcaga caacgaaatg
     1201 catctctcag acccgcaaaa atgccccgaa atgcagtttt tataacctca ttttctgctg
     1261 catcctccca gaatcagttt tttataacct cattttccta ctacaaccgc tctggaatca
     1321 gttttcatag atctcaccgt ccacaattct taccacacga cccgcatgtt ttgccacatt
     1381 cagatcatgg gtaatcatca caatggtatt tcccatctga ttcagatttt caaagagctt
     1441 caatacctct tttccgctgt tcacatcgag cgcgcctgtc ggttcatctg caagcagaat
     1501 ggcaggtttt ccgaccagcg cacgcgcaat cgccacgcgc tgctgctgac caccggaaag
     1561 ctcattgggc ttatgtccga tacgctgtga aagcccgagc atgtcaatcg tgtccatact
     1621 cgcctcctgc gcttccgcaa gccccattcc gcgtatcaga agcggcataa cgatattctg
     1681 cagcacgttg taggtcggaa tcagatgata tttctgaaaa atgaaaccta ttttctgatt
     1741 ccggattttt accagttcct tttccttcgc ctgttcgatt gccacgccat ccagcagata
     1801 gtttccgctg tccatcacat ccatgcaccc gatgatattc atgagcgtac tttttccgga
     1861 accggaagga cccaaaatgg caagatattc tccctgcttt acggtcagcg acacatcttt
     1921 gagcacatgg agggactgcg ttcccatgcg gtaggatttg ttgatatttc tcattgtgat
     1981 tacgtctgta ttttctatac tttctgcttt tgtggccgcg cagatcgccg gctccatatc
     2041 catcactgca tccatatctc tacaataacc tcctcaccgt cttcgctggt cactatcaga
     2101 agcttcagca gatcgccagc cgccagccgc tgaaacgtcg tctcggtacc tgccgtggtg
     2161 tgcaccaccg tgctgaccgg aatcaatgtt gtcacacttt taccactcat gccgccattt
     2221 ccgcccggtg ctcctccacc gttacttcct gttccgtccg atgtcgcatc gcccnnnnnn
     2281 nnnnnnnnnn tgtcgccatt ttcttcctgc ggcatcccgt acgacatcac cgtgctatca
     2341 tttgttccgg aagttccctg cgacattctg ccaggcgccc ccgagccatc tcccggcgcc
     2401 cctgaaccat cacttcctcc aggcgctccc gagccatctt ctggcgctcc tgcggtatca
     2461 cttcctcccg gcgcccctga accatcactt cctccaggca cccccgagcc atctccgcct
     2521 ttttctgcat tctcggtgtc ttccgtgctg cttgcaagat atgcctccac caccgattca
     2581 tcgacttcca tatatgtcaa ttcatttccc tcgatgctgg tcacataagc atacacatat
     2641 ttctgattat ctgccagcgt ctccatgtct gctgtcctgt gggtccatgc gaaaatacct
     2701 cctccgccga gcactgctgc aagcgccacg atgccgcccg ccatcacttt cttccttatc
     2761 atgccaaacc tcttttcaat tatttattcc gcaccaagtg cctccaccgg aaccagattg
     2821 gacgccttat acgccggata aaatccgaag atcgttcctg ttccaaccgc aaacacgaga
     2881 gcaatcaaaa atgctgatgc cgacagttcc acacggacgc cgaggtactg tacaatcggc
     2941 gccacagcca gcgatgccag attccccagc acaccaccga tgagactgat tgccgccgcc
     3001 tccgcgagga actccagcag aatgtcccgc ttggaacagc cgatcgcttt taagataccg
     3061 atctcattgg tccgctcttt tacagataca aacaatacgt tcataatgcc gattccaccg
     3121 accacgaaga cgatcgccgc catcgcaatg agaagcatcg tcagaatctg gttggaagcc
     3181 tccgccgcct ccatcttgct tcctgcatcc tcaaatgtga actcagcgtt cgggtaactg
     3241 gtggcaagca cttctgtcac ttcctcgatc acgccttcca cattattcac atctgccgcc
     3301 agcaccgtga tcgtcgggct gaccgatgat ccgaccagat atttcacacc ggtcgcataa
     3361 gggataaaaa tggaatcatc cgggctgata ccggacgcga ccgttcccat gctttccagc
     3421 acaccgttta ccacataaag ccggtcgtcg atatagatca cgcttccgta tgcctcaatg
     3481 gcactgccga aaatctcttc ggcaacgctt gcgccgagca cgcagacttt ttctttgttc
     3541 tcatcatcat cgtcggtgat aaagcttccg accgcgaggg agaggttact gatcgtctcg
     3601 tagttacttt tcactcctgc gatggtatag gtatcggctt catcgagatc tccgccatcc
     3661 acactggatt ttgtggtgta ggaaatggtc gctgccgaga tgccggacgc aaatgtttcg
     3721 atgtcatcca catcatcctc gctcagcgtg atattttcct gattcatgcg gtcttccata
     3781 tcgctctgtc cgccgaaaaa gcttcccata tccggcatac cgccgccgtc accggaaggc
     3841 atctctcccc ccggtgatcc gcccggcgcc tcgcctcccg atgaggaacc gcctcccata
     3901 tcgccggagg gtgctccgcc aaaaccgcct ccaaagcctc cgccggaacc gcctcccggc
     3961 gccatcattg ccatgccgga gtccgaatca tcgctgcttc ctccgccgcc aaaatctgtc
     4021 ccgccgtcgt aggaaatgtc caccgcaccg gcatttaagt ttttaaactg gtctgccacg
     4081 tccgcctgac cgcctttacc aatagcaatg accatcacaa tggtggcggc acccacgata
     4141 atgccgatcg aggtcatgac caccttaaat ttgttctgtg tgatattgat ccagaccagt
     4201 ctcaataatt cttttggctt cattctgtca cctgactttc tatcagcaca gtttcacctt
     4261 ccgtgagacc ggacgtgatt tccacgttga ccccgtcgga aaagcccgtc tccacatcca
     4321 ccatgcggat cgttccgtcc gcatccttca ctttgacata ggatgtcgtt ccgtcagact
     4381 ggaccgcctt gttggataca tacagcacat ccgtcacctg cctgctgata aatgtgacgt
     4441 ttcctgtcat gtccgtgtat attccagtga tgtccccagt aaacgcggcg gtcacattgt
     4501 aagacaccgt ggaggaaccg ctggaagatg aagtgtcaat gctctccacc tcccccgcaa
     4561 acgtctgatc ctcataggca gacagctcaa tctccacgac gtctccaact gcaatatttg
     4621 tgatgtcctc ctccgacacg gagacggtga tcgtcaccgc atccgtattg gaaaaagtgg
     4681 caaccgttgc gccggaagag atggtatctc ctgcctcata cgacagattg gtaatgattc
     4741 ccgtatactc cgcatagatc gtaccgtcat ccccgatggc agcttcaaat gcctcaagcg
     4801 cctcctccgc gtcggacagc gtgtcttttg catcggtaat atcattgtcc gccccggaga
     4861 catccacact gtactgactg gatgcatttt ttgccgagag ttctgcttct ttcagcgttt
     4921 tctccgcctc aattgacttc gtggtatagt tgtttttcgc tgccacaagc tttgtgtaca
     4981 gtttgtcgta attttcctgc tcctcggtaa gcgatgcact taagtccacg cccgcatcga
     5041 tctgctcctg atagtaattg atcagggacg ccgacgtatc caccgcttcc tgcgcgtcat
     5101 ccacagcctc ctgcagctgc tttatggcag cttcgtaggt ctcctgtgca acagatccct
     5161 ccgccgtgct cagattgtag gagtaacttg ccgatacttt ttgcttttct gcacttaagc
     5221 ccgcctcgct gacggctgtc ttcgcctccg tcaccgcttc ctcaagatct tttttataag
     5281 atgccacgct ctcctctgta agctttaaga tgggatcgcc ctccgcaacc gactgccctg
     5341 cggtaacata gaccgtttcc acctcaaggg tcgatgccgt gctggtggac gacgtcgcat
     5401 tttctcccgc actgttctgc gcggacgagt tgttctgcgt gcttgcagag gaagaatcct
     5461 ctgaaaaatc aagagtctga tccagcgtgc cgatcgttac actgccgctc tccgtcacgc
     5521 ccgccgtcag atttcctttc tccacggtta cttccttata tacggtctgc tcttcactgt
     5581 cacttcccga ctgatatgca aaggtgacgg ttcctgcggc aagcactgcc gccgcagcca
     5641 ctccggtcac cagccaaagc tttttcttct tcattctgtc tttcctcctg cttcctctgt
     5701 ctcagacgct ccgatcgtct cggtttttcc tgcggtctta gtctcttcca tttctgtggc
     5761 tccagtcgcc tgtgtttcaa acttcaccgt tgcctgtacg ccagtagtaa gtgtgccatc
     5821 tgtattgtcc acggcaacaa tgaccgcata cgtcacattg gacatgcttc cgccggatgt
     5881 tttttccgat gcgatggaat agacggttcc gtcgattgct cccattctgc ttccactgat
     5941 ctgtacctgc acggtgtcgc cgaccgcaag ttttgcaata ttgttctgtg atacctccac
     6001 agaaatcaga atcgtatcca gatcgtaata gcttgcaaat gcggta
//
LOCUS       DBMDQJ010000042         6042 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5284_length_6042_cov_1.388992, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000042 DBMDQJ010000000
VERSION     DBMDQJ010000042.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6042)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6042)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6042
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5284_length_6042_cov_1.388992"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="ACZO9R_01505"
     CDS             <1..190
                     /locus_tag="ACZO9R_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911782.1"
                     /translation="FVKKKESADEMHKSPADGDTTEEHSAEGDSVERDAAEGDAAEES
                     SVEKNTVKEACENETTGD"
     gene            171..932
                     /locus_tag="ACZO9R_01510"
     CDS             171..932
                     /locus_tag="ACZO9R_01510"
                     /inference="COORDINATES: protein motif:HMM:NF026158.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HIR0911783.1"
                     /translation="MRRLGIDLSYFFTYEDEIPDGVGFSHFGPVHLLWLGVCAGLLLL
                     FLHSYKRWGGRRRLLAERGIGIFLVGLEVYRIAVLALIGKMSLYQLPLHLCSMAGFLC
                     CLHAFFKWDWLGQVLYTLCLPGTVLALLFPDWVRYPAIHFITIQGFTFHAGIVLYVIC
                     QLLQHNIIPRLAVLWKVIVFLLAVVPPVYLFDKKFHANYMFVNVPSPGSPLEWLASFL
                     GNPGYLAGYAVLMLLCMVLMDAGYRVWVDRKSCGT"
     gene            993..2717
                     /locus_tag="ACZO9R_01515"
     CDS             993..2717
                     /locus_tag="ACZO9R_01515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR0911784.1"
                     /translation="MRRNEEHMADFTFSTLKYERPDFKELELLFEDLVKQVQEAVSYE
                     GLKELLKKAEHVQNKLMTACTIASIRHTLDTTDTYYEKEDEYINNTIPTVMPKFLAFD
                     AAVMNSPFRDDIEKEYGKQYFAQKELNRKTFCEANIPLMQQEAKLTNEYQKIMATAAI
                     EFEGQTLNLYGVQKYFEHEDREVRRAAVRAYSDFYHSNEKRLEEIWEELITIRNQMGK
                     NLGYANFIPVGYMQQGRTDYGEKEVAAFREQVRTELVPLCEQLYAAQAKRIGVDTLMF
                     YDEKRVFPDGNAVPAGDDDFMVEEARKMYHKISPETGEFIDFMIEHELMDLKNKPGKA
                     ATGYMTFLPDYRAPFVFSNFNQTIFDMQVLTHELGHAFAGYMAMREQPITEYYSESTD
                     IAEIHSMSMEQFSYPYAEAFFGKYADKFRFAHLQEAITFVPFGVAVDEFQHICYANPE
                     LTPKERTAEWKKLEEKYMPWRKYDADDFFDRGGFWYHKLHIYLYPFYYINYTLTTMGA
                     MEFKKKNYENHETAWQDYLNLCKCGGSMSYLETLRYANLSNPFEPGSVARAMEVAKQE
                     LMNSPFMR"
     gene            2765..3076
                     /locus_tag="ACZO9R_01520"
     CDS             2765..3076
                     /locus_tag="ACZO9R_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911785.1"
                     /translation="MEEMLEVMKKQLKMQKLISGLLAVMLVILLIAGGFFVNRMSQMT
                     AAMSEAADKLQQVDVDGINDAIGTTQEMLESAGELSDAVDSVTLKVKEFNDWMSGLFG
                     N"
     gene            complement(3083..3883)
                     /locus_tag="ACZO9R_01525"
     CDS             complement(3083..3883)
                     /locus_tag="ACZO9R_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033735 - aspartate dehydrogenase
                     [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase domain-containing
                     protein"
                     /protein_id="HIR0911786.1"
                     /translation="MKIRFGIVGCGFLGNIIANAWKDGFLEDYELVGVTSRTQESRDK
                     TAAETGCRSCRDLAELLSLEPEYIVEAASVEAVREIACTVLEHGVNLVLLSIGAFADA
                     AFYGQVKDAARRGCAKVHLANGAIGGFDVLQTITLMAQAGKLDETAGIETHTGARGFR
                     NTPVWAEHLLTDTEKTTVFTGSAKEAIVTFPRRVNVAVATSLATTGPDITQVTMHSVP
                     GWTGDDHCITAEIDGVKATVDICSSTSAIAGWSAVALLRNLASPVCFY"
     gene            complement(3900..4664)
                     /locus_tag="ACZO9R_01530"
     CDS             complement(3900..4664)
                     /locus_tag="ACZO9R_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR0911787.1"
                     /translation="MGKSAFKPGNMLYPLPAVMVSCQYPGASDPKCGNPALAGRPNIL
                     TVAWAGTVCTNPPMLSISVRPERYSYHMIETAGEFVVNLTTEALVRATDYCGVRSGRD
                     VDKFKEMHLTPLASREVSVPGIAESPVNIECRVRQITPLGSHNLILADVVAVTIDDAY
                     MDEAGKFHLNDTGLITYSHGEYFLLGKKLGTFGYSVRKDTPGKRSNSLKGDDRKKKSR
                     KNKSFPQKPSRVKEKLPGQKGILQQKNMKTDQNNFR"
     gene            complement(4705..5220)
                     /locus_tag="ACZO9R_01535"
     CDS             complement(4705..5220)
                     /locus_tag="ACZO9R_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HIR0911788.1"
                     /translation="MAEPFTIYKLTILNMLDKVDFPLTNTQISDFFLEQEYTDYFRVQ
                     QVLNDLADSGLIRTESTHSNTQYYITAAGRETLGFFRDKLTDAIEHDTIAYFEKNKMA
                     LRTVNSIIADYYKTPNQDYAVRCQFRVHDTNLIDLTLTVKNKEQAEAICNNWKKQNED
                     VYAYLMDILMQ"
     gene            complement(5213..>6042)
                     /gene="mnmL"
                     /locus_tag="ACZO9R_01540"
                     /gene_synonym="ytqA"
     CDS             complement(5213..>6042)
                     /gene="mnmL"
                     /locus_tag="ACZO9R_01540"
                     /gene_synonym="ytqA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080069.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA modification radical SAM protein MnmL/YtqA"
                     /protein_id="HIR0911789.1"
                     /translation="AQNEIYREVYESQTGGSGDFAASPQQSVTGQIAQAKLLLREKTN
                     CRKYIAYFQAYTNTYAPVGVLRALYTEALAQPDIVALSIATRCDCLPPDVLDLLAELN
                     TIKPVWVELGLQTIHEHTLRFIRSGFSLAQYEQAVSALHARGIKVITHLILGLPGETT
                     DDMRASVRYLAAHPVFGVKLQLLHVLEGTDLGTLYKNDPFPLFTLEEYCDLIADCLAL
                     LPPEMVIHRLTGDGPRRLLLAPQWSTDKKRVLNTIHRQLAGRDLWQGKYYRNEEFHG"
BASE COUNT         1419 a         1340 c         1799 g         1484 t
ORIGIN      
        1 gttcgtgaag aagaaagaat ctgcggatga gatgcataag agcccggcgg atggagatac
       61 tacggaggaa cattcggcag aaggagactc tgtggagaga gacgctgcgg agggagacgc
      121 tgcggaagaa agttccgtgg agaaaaatac ggtgaaagaa gcgtgcgaaa atgagacgac
      181 tggggattga tctttcctat tttttcacat acgaagatga gataccggac ggggtgggat
      241 tttcccactt tggtccggta catttattat ggctgggcgt gtgcgcaggg ctcttactgc
      301 tgttcctcca ttcttataag cgatggggag ggcggcgcag gcttctggcg gagagaggaa
      361 tcggcatttt cctggtgggc ttggaggtgt accggattgc ggtgctggct ttgatcggga
      421 aaatgtcgct gtatcaactg ccgcttcatc tgtgcagcat ggcaggcttt ttgtgctgtc
      481 tgcacgcatt tttcaagtgg gactggctgg gacaggttct ctataccctg tgtctgccgg
      541 gaaccgtgct tgcgctttta ttcccggact gggtgcgcta tccggcgatt cattttatca
      601 cgatacaggg atttaccttt catgccggga ttgtgctcta cgtgatctgc cagctgttgc
      661 agcacaacat tatcccgcgg cttgcggtac tgtggaaggt gatcgtattt cttctggcgg
      721 tcgtgccgcc ggtctacctg tttgataaaa agtttcatgc caattacatg tttgtgaatg
      781 tgccttcgcc cggatcgccg ttggagtggc ttgcatcgtt tctgggaaat ccgggttatc
      841 tggccggcta tgcggttctg atgcttttat gcatggtgct gatggatgcc ggttaccggg
      901 tgtgggttga cagaaaaagc tgcggaacgt agaatagaac agaaaaagag aggcgctgtg
      961 acactgcatg ggagaagaat gcctgcggtg gcatgcgcag aaatgaggag catatggcag
     1021 attttacatt ttcaacgctg aagtatgaga gaccggattt caaggaattg gaactgcttt
     1081 ttgaggatct ggtaaaacag gtacaggagg cggtctccta tgagggatta aaggaactct
     1141 taaagaaggc agagcacgtg cagaataagc ttatgactgc gtgcacgatt gcgtccatcc
     1201 gccatacatt agatacgacg gatacttatt atgagaagga agacgaatat atcaacaata
     1261 ccataccgac cgtgatgccg aagtttcttg cattcgatgc ggcagtgatg aacagtccgt
     1321 ttcgcgatga tatcgagaaa gagtacggga aacagtattt tgcacagaag gaactgaaca
     1381 ggaagacctt ctgtgaggca aacattccgc tgatgcagca ggaggcgaag ctgacaaacg
     1441 aatatcagaa gatcatggca acggcggcga tcgaatttga agggcagacg ctcaatctct
     1501 acggggtaca gaagtatttt gaacatgagg acagagaagt ccggcgtgcg gctgtgcggg
     1561 catattctga tttttaccac agcaatgaga agcggctgga ggagatctgg gaagaactca
     1621 tcaccatccg caatcagatg gggaagaacc tcggctatgc caactttatt ccggtggggt
     1681 acatgcagca gggacgcacg gattatgggg aaaaagaggt ggcagcgttc cgcgagcagg
     1741 tgcgtacgga actcgtaccg ctgtgtgaac agctctatgc ggcgcaggcg aagcggattg
     1801 gtgtggatac attgatgttt tatgatgaga agcgtgtatt tccggatgga aatgccgttc
     1861 ctgcgggaga tgatgatttt atggtggaag aggcaaggaa gatgtaccat aagatcagcc
     1921 cggagactgg agagttcatt gattttatga ttgagcacga actgatggat ttgaagaata
     1981 agccgggaaa agctgctacg ggatatatga catttctccc ggattatcgg gcaccgtttg
     2041 tgttctccaa ttttaatcag acaatctttg acatgcaggt gctgacacac gagctgggac
     2101 atgcgtttgc ggggtacatg gcgatgcggg agcagccgat cacagagtat tattccgagt
     2161 ccaccgacat cgcggagatc cattccatgt cgatggagca gttttcctac ccgtatgcgg
     2221 aggcattttt cggaaagtat gcggataagt tccgctttgc acatttgcag gaggcgatca
     2281 catttgtacc gttcggcgtg gcggtggatg agttccagca catctgctac gccaatccgg
     2341 aactgacgcc gaaggagcgc accgcagagt ggaagaagct ggaagaaaag tatatgccgt
     2401 ggagaaaata cgacgcggat gatttcttcg accgtggcgg tttctggtac cacaaactgc
     2461 atatctatct ctatccgttc tactacatca attatacgct gaccacgatg ggggcgatgg
     2521 agttcaaaaa gaaaaattat gagaaccatg agacggcatg gcaggattac ttaaatctct
     2581 gcaaatgcgg cggaagcatg agctatctgg agacattgcg ctatgcaaac ctgtcgaatc
     2641 cgtttgagcc gggcagcgtg gcgcgtgcga tggaagtagc aaaacaggaa ctgatgaaca
     2701 gtccgtttat gagatagtat agaagcagtg ttggatgagt gagaatcgga ggcaagattg
     2761 taatatggaa gaaatgttgg aagtgatgaa aaaacagttg aaaatgcaga agcttatcag
     2821 cgggctgctg gcggtaatgc tggtaattct gcttattgcg ggtgggtttt ttgtcaaccg
     2881 gatgagtcag atgacggcag caatgagtga ggcggcagat aaactgcagc aggtcgatgt
     2941 ggatggtatc aatgatgcca tcggaacgac gcaggaaatg ctcgagagtg ccggagagct
     3001 ttccgatgcg gtcgattccg tgacattaaa agtaaaagag ttcaatgact ggatgtcagg
     3061 actttttgga aattagcagg cgttaataaa aacaaaccgg cgaggcgaga ttgcggagca
     3121 gggctaccgc gctccagccg gcgattgcgc tggtggaaga acagatgtcc acggtggctt
     3181 tgacgccgtc gatctctgcg gtgatgcagt ggtcatcgcc cgtccagccc ggaacggagt
     3241 gcatggttac ctgcgtgatg tcaggtcctg tcgtggcgag cgaggtggcg acggcgacgt
     3301 tgacgcggcg cggaaaggtt acgatcgcct ccttggcgct gccggtaaag acagtcgtct
     3361 tttcggtatc ggttaaaagg tgttccgccc agaccggtgt attgcgaaaa ccgcgggcac
     3421 cggtgtgcgt ctcgatgccg gcggtctcgt caagctttcc cgcctgcgcc attaaagtga
     3481 tcgtctgtaa gacgtcaaaa ccaccgatcg cgccgtttgc cagatggact ttggcgcatc
     3541 ctctgcgggc tgcgtccttg acctgcccgt aaaaggcagc gtcggcaaac gcaccgatcg
     3601 agagaagaac gaggttgacg ccgtgttcca gtaccgtaca tgcaatttca cggacagctt
     3661 ccacggacgc tgcctccaca atatattccg gctctaagga gagcagttct gcaagatccc
     3721 gacaggatct gcatccggtt tccgccgcag tcttatcgcg cgattcctgc gtgcggctgg
     3781 tcacgccgac gagctcgtaa tcttcgagga aaccgtcctt ccaggcgttt gcgataatgt
     3841 ttccgagaaa accgcagcct acaataccaa atcttatctt cataggaaaa atcccttcgt
     3901 tatctgaaat tattttgatc tgttttcatg tttttttgtt gcaggattcc tttttgtcct
     3961 gggagtttct cctttacacg ggatggtttt tggggaaaag acttattttt cctgctcttc
     4021 ttttttctgt catccccttt gaggctgtta cttcttttgc ccggggtgtc tttccggacg
     4081 ctgtagccga atgtgccgag ctttttcccg agaagaaaat actctccgtg cgagtaggtg
     4141 atcagcccgg tgtcgtttaa gtggaatttg ccggcttcgt ccatatacgc gtcgtcgatg
     4201 gtgacagcga cgacatccgc aaggatcaga ttgtggctgc cgagcggcgt gatctggcgc
     4261 acacggcatt cgatattgac gggactctcg gcaatgccgg gaaccgatac ctcacgggag
     4321 gcaagcggcg tcagatgcat ctccttgaac ttgtcgacgt ctctgccgga gcgcacgccg
     4381 cagtagtcgg tggcacgtac aagcgcttcg gttgtgagat ttaccacgaa ctcaccggcg
     4441 gtctcgatca tgtggtagga gtagcgctcc ggacgaacgg agatggagag catcggcggg
     4501 ttcgtgcaga cggtgcctgc ccaggcaacg gtaagaatgt tcggtctgcc tgcgagggca
     4561 ggattgccgc attttggatc ggatgcgccg ggatactggc agcttaccat gacggcaggc
     4621 agcggataga gcatgttgcc gggtttgaaa gcactttttc ccataaaata aggactccct
     4681 tctgtctgtg aaaaatatct gtgtctattg catcagaatg tccatcagat atgcgtagac
     4741 atcctcgttc tgttttttcc agttgttgca gatcgcctcc gcctgttctt tgtttttgac
     4801 ggtcagcgtc aggtcgatca ggttggtatc gtggacgcgg aactgacagc gcacggcata
     4861 gtcctgattc ggcgtcttgt aatagtctgc gatgatcgag ttgacggtgc ggagcgccat
     4921 tttatttttt tcaaaatagg cgatggtgtc atgctcgatc gcgtccgtga gcttgtcgcg
     4981 gaaaaagccg agcgtctcac gccccgcggc ggtgatgtaa tactgcgtgt tgctgtgtgt
     5041 ggattccgtg cggatcagac cggagtctgc gagatcgttt aagacctgct gtacccggaa
     5101 ataatccgtg tattcctgct ccagaaaaaa gtcggagatc tgcgtgttgg tcagcggaaa
     5161 atcgaccttg tcgagcatgt ttaagatggt cagtttgtat atggtaaatg gttcagccat
     5221 ggaattcctc atttctataa tatttgccct gccagagatc gcgtcctgcg agctgtctgt
     5281 ggatggtgtt tagcacgcgc tttttgtccg tgctccattg cggagcgagg agcagacggc
     5341 gcggaccgtc accggtcaga cggtgaatga ccatttccgg cggaaggagg gcaaggcagt
     5401 ctgcgataag gtcgcagtat tcctccagcg taaaaagtgg aaacgggtca tttttgtaga
     5461 gtgttccaag atcggtgccc tctaagacgt gaagcagctg cagctttacg ccgaagacgg
     5521 ggtgcgcggc aagatagcgc acggaagcgc gcatgtcatc ggtggtctcc cccggcagcc
     5581 cgaggatcag atgggttatg actttgatgc cccgcgcgtg aagcgcggat actgcctgct
     5641 catactgggc gagggaaaaa ccactgcgta taaaacgcag ggtgtgttca tggatagtct
     5701 gaagaccgag ttcgacccag accggtttta tggtatttaa ttctgctaac agatctaaaa
     5761 catcgggtgg cagacagtca catctggtag cgatggagag cgccacaatg tctggctgtg
     5821 cgagagcttc cgtataaagc gcacgaagaa caccgacggg agcgtaggtg ttcgtgtatg
     5881 cctgaaaata ggcgatatat ttgcggcagt ttgttttttc acggaggaga agcttcgcct
     5941 gcgcgatctg cccggtgaca gattgctgcg gggaggcggc aaaatcaccg cttccgcctg
     6001 tctgagattc atatacctcg cggtaaatct cattttgggc aa
//
LOCUS       DBMDQJ010000043         6028 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5293_length_6028_cov_1.673190, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000043 DBMDQJ010000000
VERSION     DBMDQJ010000043.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5293_length_6028_cov_1.673190"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1395)
                     /gene="thrC"
                     /locus_tag="ACZO9R_01545"
     CDS             complement(<1..1395)
                     /gene="thrC"
                     /locus_tag="ACZO9R_01545"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR0911790.1"
                     /translation="MELVYASTRDAQERVTASQAILRGLANDGGLFVPTQIPALDVPM
                     EELGKMSYQETAYEVMKRFLTDFTEEELKHCINSAYDSKFDTEEIAPLVEADGAYYLE
                     LFHGSTIAFKDMALSILPYLLTTAAKKNHVKNEIVILTATSGDTGKAALAGFSDVPGT
                     KIIVFYPKNGVSPIQEKQMVTQKGDNTFVVGIHGNFDQAQTGVKKMFSDAALAKEMDA
                     AGYQFSSANSINIGRLVPQIVYYVYAYAKLYANGVIAKDEPVNVVVPTGNFGNILAAF
                     YAKNMGIPIAKLICASNENKVLYDFFSTGTYDKNREFMLTSSPSMDILISSNLERLIY
                     RIAGNDAEKNAALMRELTTTGKYEITPEMRAQLKDFYGNYTSEAETAEVIRALYEKTG
                     YVIDTHTAVAAGVYGKYKKDTGDVDTKTVIASTASPFKFTRSVMDAIDHKYDSMSDFE
                     LVDELSRIANVAVPQ"
     gene            complement(1527..3248)
                     /locus_tag="ACZO9R_01550"
     CDS             complement(1527..3248)
                     /locus_tag="ACZO9R_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0911791.1"
                     /translation="MKKISTKVLIPVVLLAVIAVISAVTGLKNANELHTSSKEISDTT
                     VNQLMLLNDISSNFKSIDAITYRMCVSTSKDERDQLMEQVDQYRTDIQTSIDSYEALT
                     QTQEEAAAMETLRENFADYTEVYDRIADFISRGNKDKARQICNTVLAPTSENVDGVLT
                     ELEGYMEANVDAGVAEQESVYNNSRAVSGVTFVLVLVFFVVAVIGSVRTVVNPVKSVT
                     TQLNDILKEIESGNGDLTRRISLKNKDEIGQLAGGINVFLETLQQIMSRIVIDSREMG
                     EIVSRVSGSVGTANSNACDISAVMEELSATMQEVASAVNTVNTNVAQIGQDVNEITKE
                     SQDMNSYATEMQSRAEDMKEKAVANKETTSRMITDIIETLKAAIEESKSVDRVNELTE
                     EILSVSSQTNLLALNASIEAARAGEAGKGFAVVADEIRKLADSTRETANNIQSINALV
                     TQAVNKLANNSNAIVEYIDGTIMPDYDRFVENGVQYRDDAAYVNTTMDHFEEKARNLQ
                     QIMEKTVDSIRDISTAIEESANSVGNAASSTTVLVSNIDTVHSEMETNQSISDRLKGE
                     AGRFKNV"
     gene            3445..4266
                     /locus_tag="ACZO9R_01555"
     CDS             3445..4266
                     /locus_tag="ACZO9R_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911792.1"
                     /translation="MTQGVSIATKKDGTVYYRAGLYYKNKHISLGSYADETTAGNAYL
                     EALAILGSLDQLPEDYIEQPTTLSFEKIIVLLNFRNNGIYFKNPIYLRNGYFSYYLSR
                     IEELKFDVDDLFYYSSRKIQKRQGHLFVSDYGMQYSILSRYGIRPYAVAGRDYQFVNG
                     DNSDFRYSNIVIVNRYHGVLQFLKNGLPRYRAVIHINGNYQIGSYSSEEKAAIAYNKA
                     VDLARAAGIQKNFPENYVDTLSPKEYADIYTGLKISRRYLNYLEQIAQKVNSISD"
     gene            complement(4253..4741)
                     /gene="leuD"
                     /locus_tag="ACZO9R_01560"
     CDS             complement(4253..4741)
                     /gene="leuD"
                     /locus_tag="ACZO9R_01560"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HIR0911793.1"
                     /translation="MKAAKGHVFKYGDNVDTDVIIPARYLNSSDPKELATHCMEDIDK
                     DFVKKVKAGDIIVANKNFGCGSSREHAPIAIKASGVSCVIAETFARIFYRNAINIGLP
                     IIECPEAAKAIEAGDEVEVDFDSGIITDKTKNTSYQGQAFPPFMQKIIDCEGLVNYIN
                     QK"
     gene            complement(4762..6024)
                     /gene="leuC"
                     /locus_tag="ACZO9R_01565"
     CDS             complement(4762..6024)
                     /gene="leuC"
                     /locus_tag="ACZO9R_01565"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HIR0911794.1"
                     /translation="MGMTMTQKILAAHAGLPSVCAGQLIEADLDLVLGNDITSPVAIH
                     EMDKMKVDGVFDKDKIALVLDHFVPNKDIKSAQHCKCVREFACRHDITNYFDVGEMGI
                     EHALLPEKGLTVAGDVIIGADSHTCTYGALGAFSTGVGSTDMAAGMATGKAWFKVPAA
                     IKFNLTGKPAKWISGKDIILHIIGMIGVDGALYKSMEFVGDGIQYLSMDDRFTIANMA
                     IEAGGKNGIFPVDDLTKQYMEKHSKRPYVIYEADEDAEYEQEFTIDLSTLKPTVAFPH
                     LPENTHTIDEVGEIKIDQVVIGSCTNGRMDDLRVAASILKGHRVAKGLRVIVIPATQQ
                     IYLDAMEEGLLKTFIEAGAVVSTPTCGPCLGGYMGILAEHERCVSTTNRNFVGRMGHV
                     ESEIYLASPAVAAASAITGKLSGPDEIA"
BASE COUNT         1390 a         1734 c         1268 g         1636 t
ORIGIN      
        1 ctgcggaacg gctacgttcg caatccggct cagctcatcc accagttcaa aatcggacat
       61 gctgtcgtac ttgtggtcaa tggcatccat cacacttctt gtaaacttaa acgggcttgc
      121 cgtggacgca atcacggtct tggtgtctac atcaccggta tcctttttgt attttccata
      181 tacaccggca gcaaccgctg tatgtgtatc gatcacgtag cctgtcttct cataaagtgc
      241 gcggatgacc tctgccgtct ccgcctcgct tgtgtaattg ccatagaaat ccttaagctg
      301 tgctctcatc tccggcgtga tctcgtattt tccggtggtc gtcagttctc tcatcagcgc
      361 ggcattcttc tcggcgtcgt ttcccgcaat gcggtaaatc agacgctcca gattgctgga
      421 gatcagaatg tccatcgacg gtgagcttgt cagcataaat tcacggttct tgtcatatgt
      481 gcctgtggag aagaaatcat ataaaacttt gttctcgttg gacgcacaaa tcagcttggc
      541 aatcggtatt cccatatttt tcgcataaaa tgcagccaga atattgccga agttgccggt
      601 aggaacaacg acattcaccg gctcatcctt tgcgatcacg ccattcgcat agagcttcgc
      661 gtaggcgtag acataatata caatctgcgg tacaagtctt ccgatattga tggaattcgc
      721 ggaggaaaac tgatatccgg cagcgtccat ctcttttgcc agcgccgcat ccgagaacat
      781 cttcttcacg ccagtctgcg cctggtcaaa gttcccgtga atgccgacaa caaacgtatt
      841 gtcacctttc tgggtcacca tctgcttctc ctggatcggg cttacaccgt tcttcggata
      901 gaatacaatg attttcgttc ccggaacatc ggaaaatcct gccagcgctg cctttccggt
      961 atctcccgag gtggcagtca gaatcacgat ctcattcttt acatgattct tcttcgcagc
     1021 ggtcgtcagc agatacggca gaatggacag tgccatatcc ttaaaagcga tcgtggaacc
     1081 gtggaacagt tccaggtaat atgcgccgtc cgcctccaca agcggtgcga tctcctctgt
     1141 gtcgaacttg ctgtcatacg cggaattgat acagtgcttt aactcctcct cggtaaaatc
     1201 cgtcagaaaa cgcttcatga cttcatatgc tgtctcctga taggacattt tgccaagctc
     1261 ttccatcggc acatccagcg ccggaatctg tgtcggtaca aacagtcctc cgtcatttgc
     1321 cagaccacgc agaatcgcct gcgatgccgt tactctttcc tgtgcgtctc tcgtacttgc
     1381 atacactaat tccatcttca taatcctctt atctctttct tcttgtaaca tgcgatgtac
     1441 aaaaaaaatg tacagtctct tatatactta actcacatta tataagaaac tgtacattca
     1501 tgtcaatgca ctaacgtact aaaatattat acatttttaa atcgtccggc ttctcccttt
     1561 aagcggtcgc tgatgctctg attcgtctcc atctccgaat gaacggtgtc gatattggac
     1621 accagaaccg tcgtgctgct cgccgcattt ccgacactgt ttgcactctc ctcaatcgcc
     1681 gtggaaatgt cgcggatgga atccaccgtc ttctccatga tctgctgcag gttgcgcgcc
     1741 ttctcctcaa aatgatccat cgtggtattc acatacgccg catcatcccg gtactgtaca
     1801 ccgttttcta caaatcggtc ataatccggc atgatcgtcc cgtcgatgta ttcgacgatc
     1861 gcgttggaat tgttggcgag cttattcact gcctgcgtta ccagcgcgtt gatggactga
     1921 atattgttcg cggtctctct ggtggaatcc gccagctttc gtatctcatc cgcgacaact
     1981 gcaaatcctt ttccggcttc gcctgctctc gccgcctcaa tggaagcatt cagtgcaagg
     2041 aggttcgtct ggctggatac gcttaagatc tcctccgtca gctcgttgac gcgatccaca
     2101 ctcttgctct cctcaatggc agcctttaaa gtctcgatga tatccgtaat catccggctg
     2161 gtcgtctcct tgttggcaac cgctttctcc ttcatatcct ccgcgcggct ctgcatctcg
     2221 gtcgcatagg agttcatatc ctgtgattct tttgtaatct cattcacatc ctgcccgatc
     2281 tgcgccacat tggtattgac cgtgttgacg gcagacgcca cttcctgcat ggtagccgag
     2341 agttcctcca tcacggcaga aatatcgcag gcattggagt ttgctgtgcc gacactgccc
     2401 gaaacgcgcg atacgatctc gcccatctcc ctggagtcaa tcacaatacg gctcataatc
     2461 tgctgcaggg tctctaagaa cacgttgatg ccgccggcaa gctgtccgat ctcatccttg
     2521 ttcttcaaag agattctgcg tgtcagatct ccgttgccgg actcgatctc ttttaaaata
     2581 tcattcagct gtgtcgtcac actcttgact ggatttacaa cagtgcgtac cgatccgatc
     2641 accgcaacga caaagaacac cagcacaagt acaaatgtga cgccgctgac tgcccgcgaa
     2701 ttattgtata cagattcctg ctccgccacg ccggcatcca catttgcttc catataccct
     2761 tccagctccg tgagcacgcc gtccacattc tcgctggtcg gcgccagcac cgtattgcat
     2821 atctgtctcg ccttgtcctt attcccgcgg gaaataaagt ctgcaatccg gtcatagacc
     2881 tcggtgtaat cggcaaaatt ctcccgaagc gtctccattg ccgcagcttc ctcctgcgtc
     2941 tgtgtcagcg cctcatagga atcaatcgac gtctgaatgt ccgtgcggta ctgatccacc
     3001 tgctccatca gctgatcgcg ctcgtccttg gacgtggaca cgcacatccg gtatgtgatt
     3061 gcatcaatgc tcttaaaatt cgacgaaatg tcatttaaca gcatcagctg attcaccgtt
     3121 gtgtcactga tctccttgct gctggtatgc agctcattgg cattttttaa tccggttacc
     3181 gccgaaatga ctgcaatcac tgcaagcagc actaccggga tcaatacttt tgttgatatt
     3241 tttttcattt aagaatggcc ccttccaaaa tatatgacat cctgcacaca tctaactctt
     3301 cttagtatag caaaattgta tattttcgtc aatcggattt ccgctattgc atcccgtctt
     3361 tccttctgtt attctatact catcacaaaa gtgtatccta atgcttattt acataatatt
     3421 tttacggtaa acaaggagcg gtctatgacg cagggtgtat ccatcgccac aaaaaaagac
     3481 ggcacggtct actaccgtgc cggtctctat tacaaaaata aacatatcag tctcggaagc
     3541 tatgcggatg agaccaccgc aggcaatgcc tatctggaag cacttgccat tctgggctct
     3601 ttggatcagc tgccggaaga ttatattgag cagcccacaa ccctctcctt tgaaaaaatc
     3661 atcgtgcttt tgaacttccg caacaacggc atttacttta agaatccgat ctacctgcgc
     3721 aacggctatt tcagctacta tctctcgcgc atcgaggagt taaagtttga cgttgacgac
     3781 ctgttctact attccagccg caagatccag aaacggcagg ggcatctgtt cgtcagcgac
     3841 tatggcatgc agtattccat cctctcccgc tacggcatcc gcccttacgc tgttgccgga
     3901 agagactatc agtttgtcaa cggggacaac agcgacttcc gctattccaa tattgtgatc
     3961 gtcaaccgtt accacggggt gctccagttt ttaaagaacg gactcccgcg gtaccgcgcc
     4021 gtcatccaca tcaatggcaa ttaccagatc ggcagctact cctccgagga aaaagccgcc
     4081 attgcctaca acaaggccgt tgatctcgcc cgcgcggcag gcattcaaaa aaatttcccg
     4141 gaaaactatg tcgatacgct ctccccgaag gaatacgcag atatctacac cggtcttaaa
     4201 atctccaggc gctatctgaa ttatctggaa cagattgccc aaaaggttaa ttctatttct
     4261 gattgatata attaaccagc ccttcgcagt cgataatctt ctgcataaac ggaggaaacg
     4321 cctgcccctg ataggaagtg ttcttcgtct tgtccgtgat gatgccggag tcaaaatcaa
     4381 cctcaacctc atctcctgcc tcgatcgctt ttgccgcctc cggacactcg atgatcggca
     4441 gtccgatatt gatcgcgttg cggtagaaga ttctcgcaaa tgtctctgcg atgacacagc
     4501 tcacgccgga agccttgatg gcgatcggtg catgctctct cgacgaacca catccaaagt
     4561 tcttatttgc tacgataata tcgcccgctt ttaccttttt cacaaaatcc ttgtcaatgt
     4621 cctccatgca gtgtgtcgcc agttccttcg gatcagaaga attcagataa cgtgcaggaa
     4681 tgatgacatc cgtatcgaca ttgtctccgt atttaaatac atgacccttt gctgctttca
     4741 tgctctgtct cctttcctcg cctatgcaat ctcatccggt ccggataact ttccggtgat
     4801 ggcgcttgcc gccgccacag ccggacttgc cagatagatc tcagactcca cgtgtcccat
     4861 tctgccgaca aagttccggt ttgtcgtgga gacacatctc tcatgttccg caaggatacc
     4921 catataaccg cccagacacg gtccgcaggt aggcgtcgaa acgactgccc ccgcctcaat
     4981 aaaggtcttt aacaaaccct cttccatcgc atccagataa atctgctgtg ttgccgggat
     5041 cacgatcacg cgcagtccct ttgccaccct gtgtcctttc agaatcgatg ccgccacgcg
     5101 cagatcgtcc attctgccgt tggtgcagga gccaatgacc acctggtcaa tcttgatctc
     5161 tcccacctcg tcgatcgtgt gcgtgttctc cggcagatgc ggaaatgcaa ccgtcggctt
     5221 taacgtgcta agatcaatcg taaattcctg ctcatactcg gcatcctcat ccgcctcata
     5281 gataacatac gggcgcttag aatgcttctc catatactgc ttcgtcagat cgtcgacagg
     5341 gaaaatgccg ttcttgccgc ccgcctcaat cgccatattt gcaatggtaa aacggtcgtc
     5401 catggaaaga tactgtatgc catccccgac aaattccatg gatttgtaca gagcaccatc
     5461 cacaccgatc atgccgatga tatgtaagat gatgtcctta ccgctgatcc actttgccgg
     5521 ctttccggtc aggttaaact tgatggcagc aggcacctta aaccacgctt ttccggttgc
     5581 cataccggca gccatatccg tgctgccgac gcctgtggaa aatgcgccga gcgccccgta
     5641 ggtacaggta tgtgaatccg ctccgatgat gacatctccc gccaccgtga gtcctttctc
     5701 cggaagcagc gcgtgctcga ttcccatctc tcccacatca aaataattcg taatgtcatg
     5761 gcggcaggca aattcacgga cacatttgca gtgctgcgca gacttaatgt ccttattcgg
     5821 cacgaaatga tccagcacga gcgcgatctt gtccttgtca aaaacgccgt ccaccttcat
     5881 cttatccatc tcatgaatcg ccaccggcga tgtaatatca tttccgagaa ccagatccag
     5941 atctgcctca atgagctgcc ctgcacagac agacggaagt ccggcatgtg ccgcaagaat
     6001 cttctgtgtc attgtcatac ccatattc
//
LOCUS       DBMDQJ010000044         5986 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5337_length_5986_cov_1.705183, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000044 DBMDQJ010000000
VERSION     DBMDQJ010000044.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5986)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5986)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5986
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5337_length_5986_cov_1.705183"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="ACZO9R_01570"
     CDS             <1..696
                     /locus_tag="ACZO9R_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter permease"
                     /protein_id="HIR0911795.1"
                     /translation="QNLYLYSISGAGLGEFVGWMAPYTLVSGVLLLLVVFVLSAGKRR
                     ISLETGALLQTSGAAGRKKNGIYLALFVLSLLSVARVLPWELMLAVVLIVLIFTDRAV
                     FLQVDYCLLFTFISFFIFIGNMGNVEAVRSVLQSLVAGRELAVGIGASQIISNVPATL
                     LLADFTHDLKNLTIGVNLGGLGTLIASMASLISYKIYAHNYNKTKWHYLLWFTVANVL
                     FLAVLVLVYVFIS"
     gene            680..1657
                     /locus_tag="ACZO9R_01575"
     CDS             680..1657
                     /locus_tag="ACZO9R_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR0911796.1"
                     /translation="MFLSHRKWQQYGWENPAEERMENSIEERMENTTETRMGGMDMSD
                     KKMNAESLRGTVCVLLSAVCFSTGGVLIKSIPWSSVTIQGARSIFSALVVGCYMLLRR
                     QKFVWNKTVLFGAVCNTVMAFAFVAATKLTTAANAIVLQFTEPVFVILLMWLIFHKKP
                     GRDAVFACAGVFAGILCFFYTSLDAGAMAGNLLAILSGLAYALVMMQKKFRGADFESS
                     LLVSCALSAAIGIPFYGQESEMSLHIWFFVLLLGVVQFGLSYVFLSRGLDAVSPVTAS
                     LTSTIEPILNPILVAVFYGETIGATAVIGALLVVGSATVYNVRQAKNAA"
     gene            2105..3529
                     /locus_tag="ACZO9R_01580"
     CDS             2105..3529
                     /locus_tag="ACZO9R_01580"
                     /inference="COORDINATES: protein motif:HMM:NF018032.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HIR0911797.1"
                     /translation="MQTEEILSAGIDIGTSTTQLVFSRMILQNTGGFGRIPQIKVVSK
                     EVIYESEIYFTPLSSREEIDGERVHEIIKKEYEKAGVCPADLATGAVIITGETSRKRN
                     AESVVRAIADVAGEFVVATAGADLESVLAGKGAGAADLSQKTGKRVVNLDIGGGTTNI
                     CVFEDGNMVDTACLDIGGRLIRVKDGRVIYMAPKLQWLCGRLEIDLAEGGTAEPEKLR
                     RLTAAMAELLAEAVHLAPEAAELSYMQTNHLLADGTPPDIVMFSGGVAACFGEEEDLF
                     RYGDIGILLAQAIRKNEAFRRAAVTDARETMRATVIGAGNYSMNISGSTIEYTTKPFP
                     LKNIPVVKLLLEREEEIEKLPENMHRALQLYREDQEKDRQVALAMKGLKCPTFAQVQR
                     IAELIADQYVRECGMGRHLILVLEEDIGKAIGQALHHRLKEKCSVICIDGISCGSGDF
                     IDLGEPVASGNVIPVIVKTLIFNG"
     gene            3539..4906
                     /locus_tag="ACZO9R_01585"
     CDS             3539..4906
                     /locus_tag="ACZO9R_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HIR0911798.1"
                     /translation="MILKTRMLGQTYEFKDVKDVLAKASEKKSGDELAGVGAESALER
                     IAAKEVLAQLTVADLTEHPVVPYEEDAVTRIIQDDIDETVYGTIKDRTIQEMREWILC
                     DVTDSGALMRASRGMTSEVIAGISKIMSNMDLILAGSKIRVTATCNTTIGTENVLASR
                     LQPNHPVDDVEGVAASTLEGLSYGIGDAVIGLNPAIDTVDSTLAIWKVLGDIRDKYEI
                     PTQTCVLSHVTTQMEALRTGQGSADLCFQSIAGSEAALSAFGINTGMLSEAEMLFREE
                     GPAKGPNVMYFETGQGSELSSSAAFGSDQQTMECRCYGLARHYHPFLVNTVVGFMGPE
                     YIYDTKQLTRAALEDVFCGHLHGLPMGCDVCYTNHMPTDQNDSETILTLLGTAGVHYV
                     MGLPQADDIMLMYQSTSYHDVASIRQLLKKEPIPEFKAWLEKRGIWENGHLGPTAGDP
                     SIFFK"
     gene            4921..5706
                     /gene="eutC"
                     /locus_tag="ACZO9R_01590"
     CDS             4921..5706
                     /gene="eutC"
                     /locus_tag="ACZO9R_01590"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HIR0911799.1"
                     /translation="MSEKETACLRDITETDIMEYMQVEHPHNEKELWKMKHATPARIG
                     IGRCGARYTTEAQLRFLAAHAAANDAVFNEVPEEVVKELGVFEVRTRCANKNEMLLRP
                     DWGREFLPDARQKIVENCVHHPQVQIYFGDGLCSPSIRANIPDLFPTIKMGLEDKGIT
                     VGTPFFVRYCRVNTARTVGPLLDAEVTCVLIGERPGLLTSESMSAYIAYHARPDMLES
                     EYTVVSNISRHGVPPVEAAAHIADLIAQILEKKKSGVEFSKAE"
     gene            5790..>5986
                     /locus_tag="ACZO9R_01595"
     CDS             5790..>5986
                     /locus_tag="ACZO9R_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR0911800.1"
                     /translation="MEKNEGGQLVRALGLKESISMTIGTVVGVGLFTCGSSQIGYVGS
                     GIIVITFLSLLISIWPCLIYG"
BASE COUNT         1436 a         1318 c         1915 g         1317 t
ORIGIN      
        1 cagaatctgt atctttacag catctccggt gccgggctgg gtgaatttgt cggatggatg
       61 gcaccgtata cgctcgtgtc aggtgtgctt ctgctgcttg tcgtgttcgt gctgagcgcg
      121 ggcaagcgcc ggatctcgct ggagacgggg gcacttttgc agacgtccgg agcagccggc
      181 aggaagaaaa acgggattta tctggcgctc tttgtgctaa gcctgctttc ggtagcgcgg
      241 gtgcttccgt gggagctgat gctcgccgtg gtgctcattg tactcatttt tacggatcgt
      301 gcggtgtttt tgcaggtgga ttactgtctg ctgtttacgt ttatcagttt cttcatcttc
      361 atcggcaata tggggaatgt cgaggcggtg aggtctgtcc tgcaaagtct tgtggcgggc
      421 agggaactgg cggtcggcat cggggcgagc cagatcatca gcaatgttcc tgcgaccctg
      481 cttctggcgg attttacgca cgatctgaag aatcttacga tcggcgtgaa tctgggcgga
      541 ctgggaacgc tcatcgcatc gatggcgagc ctgatctctt ataagatcta cgcgcacaat
      601 tataataaga cgaaatggca ctacctcctg tggtttacgg tcgcaaacgt actgtttctg
      661 gcagtactcg tgctggtcta tgtttttatc tcataggaag tggcagcagt acggatggga
      721 gaatccggca gaagagcgga tggagaattc gatagaagag cggatggaga atacgacaga
      781 aacccggatg ggaggaatgg atatgagtga taaaaaaatg aatgcggaaa gtctgcgggg
      841 aacggtctgc gtactgttat cggcagtctg tttcagcacc ggaggtgttt taatcaagtc
      901 gattccgtgg tcatcggtga cgatccaggg agcgcgcagt atcttctcgg cgctggttgt
      961 gggatgttac atgcttctgc gccgccagaa gtttgtgtgg aataagacgg tgctcttcgg
     1021 tgcggtgtgc aatacggtga tggcgtttgc ctttgtggcg gcgaccaagc tcacgacggc
     1081 tgccaatgcg atcgtgctgc agttcacgga gccggtgttt gtcattctcc tgatgtggct
     1141 gatctttcac aaaaagccgg ggcgggatgc ggtgtttgcc tgcgcgggag tctttgccgg
     1201 gattctctgc ttcttttata cgtcgctgga tgccggagcc atggcgggga atctgcttgc
     1261 gattctctca ggacttgcct atgcgcttgt gatgatgcaa aagaaattcc gcggcgcgga
     1321 ctttgaatcg tcgcttctgg tgtcctgcgc gttgagcgcg gcgatcggaa tcccgtttta
     1381 cgggcaggag agcgagatgt cactgcatat ctggttcttc gtgctgctgc tcggcgtggt
     1441 gcagttcgga ctgtcctatg tgttcctgtc gcggggactg gacgcagtct ctcctgtcac
     1501 ggcgtcgctc acctcgacga tcgagccgat cttaaatccg attctggtgg ctgtttttta
     1561 cggggagaca atcggcgcaa cggctgtcat tggcgccctg ctggtggtcg gttcggcgac
     1621 ggtctacaat gtaaggcagg caaaaaacgc tgcctgaatc cgtacaaaaa ggctggaaga
     1681 agtcatacaa aaggtacaaa aaagacaaaa atatatgagc agccggtaaa atatttgtta
     1741 aaaaaggaaa ttgctgaaca aaaagtgtca tgctataata acttctaaat tcagatatca
     1801 gatgacatgt gcaagggagc accgcggtag cggctgctct ctttttttat gtaataggaa
     1861 acttcgttcc tgttacataa aaaagctccc cggggatcgc actgcggcgg agacgaaaga
     1921 tgtcgcaaga gcgacccgcc gtaggcggag aatcctgcag gcaggattct tatttattcc
     1981 ataggaaata ccagattgca ctaagatgtg aaagccgggc tgccgcagat aaaaaaccac
     2041 cgcgcaatta tatggtgagc ggcagacaca gacagaaaag aatagttttt ggaggaggag
     2101 aagaatgcag acggaagaga ttttaagtgc cggaattgat attggaacgt cgacgacgca
     2161 gcttgtgttc agcaggatga ttctccagaa cacaggcgga tttggcagaa ttccacagat
     2221 caaggtggtg tcaaaggagg tcatctacga gagtgagatt tattttacac cgctgtcatc
     2281 ccgggaggag attgacgggg agcgcgtgca tgagatcata aaaaaggaat atgagaaggc
     2341 gggtgtttgt ccggcagatc ttgcaaccgg agccgtcatc atcacggggg agacttcacg
     2401 gaaaagaaat gccgagtcgg tagtgcgggc gatcgccgat gtggcgggag agttcgtcgt
     2461 ggcgacagcg ggagcggatc tggagtctgt tctggcagga aaaggtgcgg gagccgcgga
     2521 tttgtcccaa aagacgggga agcgggtagt caatctggat atcggcggcg gcacgacaaa
     2581 tatctgtgtc tttgaggacg gaaatatggt tgatacggca tgcctcgaca tcggcggcag
     2641 actgatccgc gtgaaggacg gcagagtgat ctacatggcg ccaaagctgc aatggctgtg
     2701 cggcaggctt gagattgatc ttgcggaggg cgggacggca gaaccggaaa agctgcgcag
     2761 actcaccgct gcgatggcgg aacttcttgc ggaggcagtg catctggcgc cggaggcagc
     2821 agagctttcc tacatgcaga ccaaccatct tctggcggat ggcacgccgc ccgatattgt
     2881 gatgttctcc ggtggggtgg cggcatgctt cggggaagag gaggatctgt tccgctatgg
     2941 ggacatcgga atccttttgg cgcaggcgat ccggaagaac gaagcgtttc gccgggcggc
     3001 agtgacggat gcgcgggaaa ccatgcgggc gacggtgatc ggcgcgggaa actacagcat
     3061 gaacatttcc ggaagtacga ttgagtacac gacgaaaccg tttccgttaa aaaatattcc
     3121 ggtggtgaag cttttgctgg agcgtgagga agagattgaa aaactgccgg aaaacatgca
     3181 ccgggcgctg cagctttacc gggaggatca ggaaaaagac cggcaggtgg cgcttgccat
     3241 gaaggggtta aagtgcccta ctttcgcaca ggtacaaaga atagcagaac tgattgcaga
     3301 ccagtatgtc agggagtgcg gcatgggcag gcatctgatc ctggtgcttg aggaggatat
     3361 cggaaaagca atcgggcagg cgctgcatca cagactgaaa gagaagtgca gcgttatctg
     3421 catcgatgga atcagctgcg ggagcggaga ttttattgat ctgggcgaac cggttgcatc
     3481 gggtaacgtc attcccgtga tagtaaagac attgatattt aatggttagg agtagcttat
     3541 gatattaaag acacggatgc tggggcagac ctatgaattt aaggatgtaa aagatgtgct
     3601 ggcaaaggcg agcgagaaaa aatcgggaga cgagctggca ggtgtgggag cggagagcgc
     3661 cttagagcgg attgccgcta aggaggtgct ggcgcagctg accgtggcgg atttaacaga
     3721 gcatcccgtg gtgccctacg aggaggatgc ggtgacgcgg atcattcagg atgatatcga
     3781 tgaaacggtc tacggcacga tcaaggatag aacgatccag gagatgcggg agtggattct
     3841 ctgtgacgtc acagacagcg gggcgctgat gcgcgcaagc cggggaatga ccagcgaagt
     3901 gattgccggg atctccaaga tcatgagcaa tatggatctg attcttgctg gaagcaagat
     3961 ccgggtgacg gcgacctgca ataccacgat cggcacagaa aacgtgctcg cgtcgagact
     4021 gcagcccaat catccggtgg atgatgtgga aggcgtggca gccagcacct tagaaggact
     4081 cagttacggc atcggcgacg ctgtcatcgg gttgaatccg gcgattgata cggtggacag
     4141 cacgcttgca atctggaaag tgctcgggga catccgggat aaatatgaga ttccgacaca
     4201 gacctgtgtg ctctcacatg tgacgacaca gatggaggcg cttagaaccg ggcagggaag
     4261 cgccgatctg tgtttccagt cgattgcggg atcggaggcg gcgctctcgg cgtttggcat
     4321 caacaccggg atgctgtcgg aggcggagat gctgtttcgg gaggagggac cggcaaaggg
     4381 acccaacgtc atgtattttg agaccgggca gggttcggaa ctgtcgtcct cggcggcgtt
     4441 cggcagcgat cagcagacga tggaatgcag atgttacggt ctggcacggc actatcatcc
     4501 gtttctggtg aacacggtgg taggtttcat ggggccggag tatatctacg acaccaagca
     4561 gctcacaagg gcggcgcttg aggacgtatt ctgcgggcat ctgcacggac tgccgatggg
     4621 ctgcgacgta tgttatacga accacatgcc gaccgatcag aacgactcgg agacgatcct
     4681 gacacttctg ggaacggcgg gcgttcacta tgtgatggga ctgccgcagg cggatgatat
     4741 tatgctgatg tatcagtcca ccagctatca cgatgtggcg agtatccggc agctgctgaa
     4801 aaaggagccg ataccggagt ttaaggcatg gcttgaaaaa cggggcatct gggaaaacgg
     4861 gcatctcggc ccgaccgccg gagatccgtc catatttttt aaataggagg aacgggattt
     4921 atgagtgaaa aagagaccgc ctgcctccgc gatattacag agacagacat tatggaatat
     4981 atgcaggtgg agcacccgca caatgaaaag gaactctgga agatgaagca tgcgacgccc
     5041 gcgcggatcg ggatcggacg gtgcggcgca cgctacacga ccgaggcgca gctccggttt
     5101 ctggcggcac atgcggcggc aaacgacgcg gtgttcaatg aagtgccgga ggaagtggta
     5161 aaggagcttg gcgtttttga ggtgcggaca agatgcgcca ataaaaacga aatgctcctc
     5221 cgcccggact ggggcaggga gtttttgccg gatgccagac aaaaaattgt ggaaaactgt
     5281 gtccatcatc cgcaggtaca gatctatttc ggcgacgggc tgtgctcgcc gtctatcagg
     5341 gcgaacattc cggatctgtt tccaaccata aaaatgggtc tggaggataa gggaattacc
     5401 gtcgggacgc cgtttttcgt gcggtattgc agagtgaata ccgcgcggac ggtcggaccg
     5461 ctcctggatg cggaggtgac ctgcgtgctc atcggagagc gcccgggtct tctgacgtca
     5521 gagagcatgt cggcatatat cgcctaccat gcgagaccgg acatgcttga gtcggagtat
     5581 acggtcgtat cgaatatcag caggcacggt gtgccgccgg tggaggcagc cgcacatatt
     5641 gcggatctga tcgcacagat cctggaaaag aaaaaaagcg gtgtagaatt ttcaaaagca
     5701 gaataaagac atggttgcga aggagcacag gcgggacgtc tgtgctcttt ttctatagga
     5761 gcagcaagac agacaaagga ggatacgtta tggagaaaaa cgaaggaggt cagctggtgc
     5821 gtgctctcgg actgaaagag tcgatcagta tgacgatagg aacggtagta ggagtgggac
     5881 tgtttacctg tggttcctca cagattggat atgtcggatc ggggattatt gtaattacat
     5941 ttttatccct gctcatttcg atctggccct gtctgatcta cggcga
//
LOCUS       DBMDQJ010000045         5966 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5354_length_5966_cov_1.593180, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000045 DBMDQJ010000000
VERSION     DBMDQJ010000045.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5966)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5966)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5966
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5354_length_5966_cov_1.593180"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(21..422)
                     /locus_tag="ACZO9R_01600"
     CDS             complement(21..422)
                     /locus_tag="ACZO9R_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR0911801.1"
                     /translation="MTKQEKAIELHDRGCNCAQAVACAFAEEIGVSEETLFAAAEGFG
                     LGMGGMEGTCGAVSGAVMLAGFKNSCADPKNPKTKAATYQLTREITRQFAEKNHALVC
                     KELKGVETGVVLRSCPDCIRDAVAIAEKVLQ"
     gene            538..1416
                     /locus_tag="ACZO9R_01605"
     CDS             538..1416
                     /locus_tag="ACZO9R_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR0911802.1"
                     /translation="MNQTTITKQRGLSGFALKYFAMACMILDHIHYFFSFTGKIPLFF
                     SWIGRLAAPLFLFCIVEGFLHTHDRKKYFLRIYAIAVFMGLVQFSFYNIASGLVRPDG
                     FFPQNQMLASFSVLLVVLWGIDRCQKKQWIRGLSAILIPVFLPFLLYGLFAVSEAFSI
                     PYGNFLLNLLVYSVLPCHTAIIDGGTATLLGGVILYLTHRHRKLQAGAFALFVLAWDI
                     LPVLLFIPAGTSASFFFTDAYEWLEVFAVIPMLCYNGTRGHGSKKLFYWFYPTHIYVL
                     YALSFLLYLTLYGMGS"
     gene            1433..2095
                     /locus_tag="ACZO9R_01610"
     CDS             1433..2095
                     /locus_tag="ACZO9R_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0911803.1"
                     /translation="MAHILAIDDDPGILALIEHALRREGHTITCMPHVPADLAAHLAE
                     YDLILLDIMMPQTDGYELCSAIRDDTDCPILFLTAKTMEEDVDYGFSVGADDYIKKPF
                     SIVELRARVAAHLRREQREKHHCLSIQGIRFLLASREVAAGGVTLPFTRTEYEITLLL
                     AKNRGHTFSREQIYEAVLGYDKTGDASAITEHIKNIRKKFAAVNMAPIQTVWGIGYRW
                     NA"
     gene            2065..3531
                     /locus_tag="ACZO9R_01615"
     CDS             2065..3531
                     /locus_tag="ACZO9R_01615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911804.1"
                     /translation="MGDRLSMERMKKKKKEIRLRTFFVRFLLKLVAAVLLAFLLWLLQ
                     ITVSVGTGLILPANAAEKEALAYLHTIGAHTDIVSSGIPSACGYAIYDASGELLETDM
                     SGSIRENADLLALSGQDSLSANMLGRTYVKRQTDTRIVVLTYDFRSAFANPALRRLFP
                     SAELLEVFLLLFLLLAAFVLVITRQAKYLSRELFHLQEAADEIRNQNLDFTIRATAIC
                     EFNQVAASLDALKSELSASLKEQWHMQQQRRRQFSALAHDIKTPLTIVRGNAELLSET
                     ALDETQQSCNRFILENAAQIQDYLARIIELAKTDDPAACSAECRFQAQLHTASFFDDL
                     LGNTKSLGQKKELHVLFSTETLPAVLPLPEHPLRRILNNLLDNAVCYSPHKGTVTLDA
                     SIRDYRDAPEKESTLFLTVSDEGPGFCEDALLYGTEAFYRESADRNDKSHFGLGLSIA
                     DQLARGLGGSITLCNAPAGGAVVIVRLPLTGQLQRDTE"
     gene            complement(3535..4599)
                     /locus_tag="ACZO9R_01620"
     CDS             complement(3535..4599)
                     /locus_tag="ACZO9R_01620"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HIR0911805.1"
                     /translation="MDIRFVKRDVLKEKPDQKHLGFGKYMTDYMFVMDWTKEDGWKDA
                     RIVPEGPISLEPACVTLHYAQETFEGMKAYRTAEGKIQLFRPEMNAKRMINSNARLCM
                     PEFPVDMFVEAVKALVKVEADWVPSEPETSLYIRPFMFATEAALGVHMASAYKFMIIC
                     CPVGAYYAEGINPVKILVEDELVRAVKGGTGFTKCGGNYAGSILGQVKAEKLGYSQVL
                     WLDGEHRKYVEEVGTMNIMFKIAGEIYTAPIEGTVLPGVTRDSMIHLLRDWGYKVNET
                     RLSVDDLMKAGHDGTLEEVFGTGTAAVISPVGELRYKDDVVTINDFKIGELTQKLYDT
                     LTGIQWGKLPDKYGWTVPVE"
     gene            complement(4936..5466)
                     /locus_tag="ACZO9R_01625"
     CDS             complement(4936..5466)
                     /locus_tag="ACZO9R_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0911806.1"
                     /translation="MKITTKQITTTAVLLAICIVSQFFKNTSVYITGPVINACLILAV
                     LSVGIPCGIILSVITPVTSFFITGSPIIGAIPAIMPCIMAGNALLVLGVGLVTKKCKG
                     NGGLIAGMAAGSVVKALFMGIVISLILIPNLLPAPMEAKMAVFQTTFSVTQLVTSLIG
                     SVYAFILWIPLKKVVK"
     gene            complement(5568..>5966)
                     /locus_tag="ACZO9R_01630"
     CDS             complement(5568..>5966)
                     /locus_tag="ACZO9R_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911807.1"
                     /translation="YGIKVIPDVIPDGYVQTDEEKTYQIGYDADGNVIDDNCVMRYEN
                     SETAGWFTIRARSTDIGEMTSYVSGGLKPMTVSGVKVTAAHYPAGSSGGTFTDGYLAT
                     YKKNGVTVNVQSEGLSRDEFCALVENLLAE"
BASE COUNT         1323 a         1764 c         1342 g         1537 t
ORIGIN      
        1 taagatgaac tcgtcatagt tcattgtagt accttttccg cgatcgcaac cgcatcgcgg
       61 atacagtccg gacagctccg caaaaccacg ccggtttcca cacccttcaa ttccttacag
      121 acgagcgcat ggttcttctc ggcaaactgt ctggtaatct ccctggtcag ctgataggtc
      181 gccgccttgg tcttcgggtt cttaggatcg gcacagctgt tcttgaagcc cgcaagcatt
      241 accgcaccgg agaccgctcc gcaggttccc tccatgccgc ccatgccgag tccgaatccc
      301 tccgccgccg caaacagcgt ctcttccgac acgccgatct cctccgcaaa cgcacacgcc
      361 accgcctgtg cacagttgca tcctctgtca tgcaattcga ttgctttttc ctgctttgtc
      421 attttataac ttcctttccg ttccttataa aatccttata atttgcctgt atgctgtttc
      481 tgtgaacatc ttatcacaac ttatcacaag aagtaaacaa ggaggattcc aggacgtatg
      541 aatcagacaa caatcacaaa acaacgggga ctctcaggct ttgcgctcaa atattttgcc
      601 atggcatgca tgatcctcga ccacattcac tattttttct catttacagg gaagatcccg
      661 ctgttctttt cgtggatcgg caggcttgcc gccccgctgt ttctcttctg tatcgtggag
      721 ggttttcttc acacgcacga ccggaaaaaa tattttctgc gcatttacgc catcgccgtt
      781 ttcatgggac tggtgcagtt ttccttctat aatattgcat ccggtctcgt ccggccggac
      841 ggctttttcc cgcagaatca gatgcttgca tccttttccg tcctgctcgt cgttctttgg
      901 ggcatcgacc ggtgtcagaa aaaacagtgg atccggggac tttctgccat cctgattccg
      961 gtctttctgc cctttcttct gtacgggctg ttcgccgtat cggaagcctt tagcattccc
     1021 tacggcaatt ttctcttaaa ccttctggtg tacagtgtcc tgccctgcca cactgcgatc
     1081 atcgacggtg gcaccgccac actgctcggc ggcgtgattc tctacctgac gcacagacac
     1141 cgcaaattgc aggcaggtgc ctttgcactt ttcgtgcttg cgtgggacat cctgcccgtg
     1201 ctgcttttta tacccgcagg aacttctgcc tctttctttt ttaccgatgc ttatgagtgg
     1261 ctggaagttt ttgccgttat ccctatgtta tgttacaatg gaacccgtgg acacggctcc
     1321 aaaaagctgt tctactggtt ttatccgacg cacatttacg tgctgtacgc actttcgttc
     1381 cttttgtacc tgacccttta tggaatgggt tcttgataga aatgaggttt ttatggctca
     1441 cattctcgca attgacgatg atcccggcat cctcgcgctg attgaacatg cactccgccg
     1501 ggaggggcat accattacct gcatgccgca tgtaccggcg gatcttgcgg cgcacctcgc
     1561 agagtatgat ctcatccttc tcgacatcat gatgccgcag acggacggct acgaactctg
     1621 cagcgccatc cgcgacgaca cggactgccc gattctgttc ctgaccgcaa agaccatgga
     1681 ggaggacgtc gactacggtt tttccgtcgg tgcggacgac tatatcaaaa agcccttctc
     1741 catcgttgaa ctgcgtgcgc gcgttgccgc acatctgcgc cgggagcagc gcgaaaaaca
     1801 ccactgtctc tccatacagg gcatccgctt tctgctcgcc tcccgcgagg tcgcagcagg
     1861 cggtgtcacg ctccccttta ccaggacgga gtatgagatc acacttctgc tcgcaaaaaa
     1921 ccgcgggcat acattttcca gggagcagat ctacgaagcg gtcttaggat acgacaagac
     1981 aggcgatgcg tctgccatta cagagcatat taaaaatatc cggaaaaaat ttgccgcagt
     2041 aaatatggcg ccaattcaga cagtatgggg gatcggttat cgatggaacg catgaaaaaa
     2101 aagaaaaaag aaatccggct gcgcacattt tttgtgcgct ttttactaaa gcttgtcgcc
     2161 gcggtgcttc tggcgttcct gctgtggctt ctccagatta ccgtctccgt cggtacgggt
     2221 ctgatcctgc ctgcgaacgc cgccgaaaaa gaggctcttg cctaccttca caccatcggc
     2281 gcacatacgg acatcgtgtc ttccgggatc ccatccgcct gcggctacgc catctatgac
     2341 gcatccggag agcttcttga aacggacatg tccggttcca tccgcgaaaa tgcagatctt
     2401 ctcgcacttt ccgggcagga ttccctctcc gcaaatatgc tcggacgaac ctatgtcaaa
     2461 cgccagaccg acacccggat tgtggtgctc acttatgatt tccggtctgc ctttgcaaat
     2521 cccgcgctcc ggcgtctctt tccgagcgct gaactgctgg aagtgtttct cctgctgttc
     2581 ctgcttcttg cagcttttgt actcgtcatc acacggcagg caaaatacct gtcgcgggaa
     2641 ctttttcatc tgcaggaagc tgccgacgaa atccgcaatc agaatctcga ctttaccatc
     2701 cgggcgactg ccatctgcga attcaaccag gtagccgctt ctctggacgc gctgaaatcc
     2761 gaactttccg cctccttaaa agagcagtgg cacatgcagc aacagcgcag acgtcaattt
     2821 tccgcgctcg cccatgacat caagacgccg cttacgatcg tccgcgggaa cgcggagctt
     2881 ttatccgaga cagcgctcga tgagacgcag caatcctgca accggtttat tctggaaaac
     2941 gccgcacaga tccaggatta tctggctaga attatcgagc tcgcaaagac ggatgatccc
     3001 gctgcatgtt ccgcagagtg ccgttttcag gcgcagcttc acacggcatc gtttttcgac
     3061 gatctgctcg ggaatacaaa aagtctcggt cagaaaaaag aactgcatgt tcttttttcc
     3121 accgagactc ttccagccgt tctgccactg ccggaacatc cgctgcggcg tattttaaac
     3181 aatctgctcg acaatgccgt gtgctactca ccgcacaaag gaacggtcac tttagatgcc
     3241 tccatccgcg attataggga tgcacccgag aaagaaagca cgctctttct cactgtatcc
     3301 gatgagggac ccggcttctg cgaagacgcc ctgctctacg gcacggaagc cttttacagg
     3361 gaatccgccg accggaatga taaaagccac ttcggtctcg gactttccat cgccgaccag
     3421 cttgcccgtg gacttggcgg atcgatcact ctgtgtaatg cgccggcagg cggagccgtc
     3481 gtaatcgtaa gacttcctct gaccggacag ctacagcgcg atacggaata aatcctattc
     3541 taccggaacg gtccagccgt acttatccgg aagcttgccc cactggatac cggtcagtgt
     3601 gtcgtacagc ttctgtgtca gctcgccgat cttgaaatcg ttgatcgtga caacatcatc
     3661 cttgtagcgg agttcaccaa ccggagagat cacggctgcg gtacccgttc cgaatacttc
     3721 ctctaaggtt ccgtcatgtc ctgccttcat caggtcatcg acggataatc tggtctcgtt
     3781 taccttgtaa ccccagtctc tcaacagatg aatcatggaa tctctggtaa cgcccggaag
     3841 taccgtaccc tcgatcggtg ctgtgtagat ctcaccggcg atcttgaaca tgatgttcat
     3901 tgtaccgact tcctcgacgt atttgcggtg ctcaccgtcc agccagagta cctgggagta
     3961 accaagcttt tctgccttta cctgtcctaa gatggagcct gcatagttgc cgccgcactt
     4021 ggtgaatccg gtaccgccct tgactgcacg caccagctcg tcctcgacaa gaatcttcac
     4081 cggattgatg ccctctgcat agtaagcgcc taccgggcag cagataatca tgaatttgta
     4141 agcggacgcc atatgtacgc cgagcgcagc ctctgttgcg aacatgaacg ggcggatata
     4201 aagagaggtc tccggctcag acggaaccca gtctgcctcc acctttacca gcgcttttac
     4261 cgcttctacg aacatatcca ccgggaactc cggcatgcag agtctcgcgt tggaattgat
     4321 cattctcttc gcattcatct ccggacggaa cagctggatc tttccctctg ccgtgcggta
     4381 tgccttcata ccctcaaatg tctcctgcgc atagtgcagc gtcacacagg caggctccag
     4441 actgatcggt ccttccggca cgattctcgc atccttccag ccgtcttcct tcgtccagtc
     4501 catgacgaac atgtaatcgg tcatatactt gccgaatccg agatgtttct gatccggctt
     4561 ctcctttaat acgtctcttt ttacaaatct gatatccatt atgctaccat tcctttcccg
     4621 acatataaaa ttcttgaaat caattatgat actttctttt ttcagtgtca agattattcc
     4681 agccgcttat gccgctcatg attgtaagtt atacattagt catcttccca tacccatatg
     4741 gaatgaagag atcaagaaca aggtgtgcgc tcgccgcaca cttgcgcgcc cgagcggtat
     4801 gcaaagcatt atttacctca ccgcgaggtg cgcatccccg cggtgcattt ttgtgtaata
     4861 ggaacaaagt ttcctattac acaaaaacgc ggagcaggat cttttctccc gctccgccat
     4921 cggagtctct tttccctatt ttaccacttt tttcaacgga atccagagaa taaatgcata
     4981 cacgcttccg atcagggaag tcacaagctg cgtcacggaa aaggtcgtct ggaacactgc
     5041 catctttgcc tccatcggtg ccggcagaag gttcggaatc agaatcagcg agatcacaat
     5101 tcccatgaac agtgccttta cgacagatcc tgccgccatg ccggcaatca gtccgccgtt
     5161 tcctttgcat tttttcgtga ccagtcccac acccaggacc agaagggcat tgcccgccat
     5221 gatacacggc atgatcgccg gaatggcacc gatgatcggg cttcccgtaa taaagaatga
     5281 tgtgaccggc gtgatcacag agagaataat gccgcaggga attcccacac ttaacaccgc
     5341 taagatcagg catgcattga tgaccggtcc ggtaatatag acgctcgtgt tcttaaaaaa
     5401 ctggctcacg atacagatcg ccagaagaac cgctgtcgtt gtgatttgtt ttgttgtgat
     5461 tttcatcgct tcctccttct gatgcagcac tccttcgcag atgtaatgca ctctcccgtc
     5521 actgcaccaa agcaagcata atacttctct ctcataaaat caagccgtca ctccgccagc
     5581 agattctcca caagtgcgca gaactcgtcc cggctcagtc cttccgactg cacattcacg
     5641 gtcacaccgt ttttcttata ggtcgccaga tacccgtccg tgaacgttcc tccactgctg
     5701 cccgccggat aatgtgctgc tgtcaccttc acaccggaaa ccgtcatcgg ctttaatcca
     5761 ccggatacat aggacgtcat ctcccctatg tccgttgagc gtgcgcgaat ggtaaaccat
     5821 cccgctgtct cactgttctc ataacgcatg acacagttgt catcgatcac attgccatct
     5881 gcatcatagc caatctgata cgttttctcc tcatccgtct gcacatagcc gtccggaatc
     5941 acatccggaa tcaccttgat tccgta
//
LOCUS       DBMDQJ010000046         5922 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5401_length_5922_cov_1.766020, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000046 DBMDQJ010000000
VERSION     DBMDQJ010000046.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5922)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5401_length_5922_cov_1.766020"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            15..1775
                     /locus_tag="ACZO9R_01635"
     CDS             15..1775
                     /locus_tag="ACZO9R_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR0911808.1"
                     /translation="MDIFGVLSLIGGLALFLYGMHVMGAGLEKMSGGRLERILENLTS
                     NPLKAVLLGAGVTAVIQSSSATTVMVVGFVNSGIMKLSQAIGIIMGANIGTTITAWLL
                     SLTGIQGDNLFINMLKPTSFSPILALIGIILLMSGKSDKKKITGEILLGFVVLMYGMS
                     AMSGAVEPLADVPEFTNLLTMFKNPVLGVLAGALLTAVIQSSSASVGILQALSVTGGF
                     TFGTAIPIILGQNIGTCVTAMLSAIGANKGAKRTAFVHLYFNMIGTVLFLCLFYLGNA
                     IFSFPFTNDVVNAADIALVHSIFNVFTTLVLLPFTKGLEKLAYLTIPKTEDEKKTAND
                     AFVILDDRFLSSPAFAIEQCRSLVSQMAEMTRDGFLEAMDVLGEYSEEKAQDVIDKEN
                     RVDVYDDKITAYLTKLSSENLSYKDSLQVTSLLHCITDFERISDHSINVVESVQQMRK
                     ENLTFSRKGEEEMNLYGAAIRDILTRTTDAFVNNDQALAHTVEPLEEVIDELNKDVKK
                     HHMKRLRKGKCSMELGLLLSDLAMNYERVADHCSNIAVYMMQLKDTQLEEHSFTEQLD
                     EAESAEFTRLLDEFGSKYAL"
     gene            1827..2498
                     /locus_tag="ACZO9R_01640"
     CDS             1827..2498
                     /locus_tag="ACZO9R_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0911809.1"
                     /translation="MATIYIVEDDVNIREIERYALKNSGYEVEEFESGESLFKRLERA
                     VPSLLLLDIMLPDEDGLEILGKLRANKNTAAIPVIMVTAKTSELDKVKGLDLGADDYI
                     TKPFGVMELISRVKALLRRTQSAAEETKICHGEIRMDNDKHAVFVGNEPCELTFKEYE
                     LLKYLMINAGIVLSRDKIMDQVWGFEYEGESRTVDMHIKTLRQKLGAAGSCIKTIRNV
                     GYMIE"
     gene            2509..3822
                     /locus_tag="ACZO9R_01645"
     CDS             2509..3822
                     /locus_tag="ACZO9R_01645"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911810.1"
                     /translation="MKKKLNSKFMLIAAIAILVTAVCSIGLFYSILVQQIYDDLKANA
                     HVISRLDFEDASQEVCDQLSEDGLRITLIASEGTVLYDSMEDETKMENHRGRPEVERA
                     LAVGEGRGMRKSTTSAEHTFYYAMRLSDGNVLRIGKESGSIYHLAWNMTMLTLGVGLC
                     VFLVCAFFAKRMTKRFVEPIEKMADNIVLVDEREVYEEMRPFVSMIKQQHMDILNHAQ
                     MRQEFTANVSHELKTPLTAISGYAELIASGMTNESDTEHFANEIHRSAERLQSLINDI
                     IKLSELDNSDLKLEFEPIDLYALATTCIDQMQIAAQKNEVTLLQEGEPVTINGNKTLI
                     EELLYNLCSNAVRYNKKGGSVTVITGMKNQRPALTVRDTGIGIPKEQQDRVFERFYRV
                     DKSRSKSTGGTGLGLAIVKHIVVQHEAQIELSSEEGVGTEVTVIF"
     gene            complement(4676..>5922)
                     /locus_tag="ACZO9R_01650"
     CDS             complement(4676..>5922)
                     /locus_tag="ACZO9R_01650"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0911811.1"
                     /translation="VALLMYFDLKLEICAGVLNMVCCILNGVYSVHVLGTNSLSETNM
                     IFVICSIIIVINTITCLASRVAVLHNTEELAELEAHKKKQEDMMNSIVTVGTSVNEST
                     QSIHALIEEMTEATGCVSQAMGDITVSMESTVASIQEQASMTGRIQDIIADTVSIADA
                     LTNISQSSGKNVETGQALVSNIVTQTAEVESENKLVKENMTALQAHTQDMLKIISIIQ
                     QISAQTNLLALNATIEAARAGEAGRGFAVVAEEIRMLSEQTKQSTENIEEIITKLNKN
                     AGDTIASMDHVMDKIGNQVSMIHDIEENFSGIRSGLTGLEQNAAAISEKTRILKETNT
                     MLVDETNTLSSTSEEISASAEETNAMCTDNAERFKSVNNVIAELAAEAGKMNGFIEEY
                     HRLHDADTDAETAISRQALHTA"
BASE COUNT         1459 a         1338 c         1689 g         1436 t
ORIGIN      
        1 caaaggagat tagaatggat atttttggag tactgtccct gattggcggg ttggcattat
       61 tcttatacgg aatgcatgtg atgggtgccg gactggagaa gatgtcaggc ggaaggctgg
      121 agcgcatcct ggagaatctg acatcgaatc cgttaaaagc ggtactgctc ggtgcaggtg
      181 tcacagcggt catacagtca tcttccgcga cgacggttat ggtcgtcgga tttgtaaatt
      241 ccggtattat gaagctttca caggcgatcg gaatcatcat gggtgccaat atcggtacga
      301 cgattaccgc atggctgctc agcttgacgg gaattcaggg agacaacctt tttataaata
      361 tgttaaagcc gacatcgttc tcaccgattc tggctttgat cggtattatt ctgctgatgt
      421 ccggcaagag cgataagaag aagatcaccg gagagattct gcttggattc gttgtgctga
      481 tgtacggtat gtcggcgatg agtggtgcgg tggagccgct tgcggacgtg ccggagttca
      541 cgaacctgct taccatgttc aagaatccgg tgcttggagt gcttgcagga gcgctgctta
      601 cggcggtcat ccagagttct tccgcttctg tcggtatttt acaggcgctt tcggtaaccg
      661 gcggatttac atttggcaca gcgatcccga ttattctggg acagaacatc ggtacctgtg
      721 tgacggcaat gctctccgca atcggtgcga acaagggtgc aaagcgtacc gcgtttgtac
      781 atttatattt caacatgatc ggtacggttc ttttcctgtg cctgttttat ctggggaatg
      841 ccattttcag cttcccgttt acgaacgacg tcgtaaatgc ggcggatatt gcgctggttc
      901 acagtatttt taatgtattt accactctgg ttctgcttcc gtttacgaag ggactggaga
      961 agctggcata tcttacgatt ccgaagacag aggatgagaa gaagacagca aacgatgcgt
     1021 ttgtaatctt ggacgacaga ttcctctcgt ccccggcatt tgcgattgag cagtgccgtt
     1081 cactggtaag ccagatggcg gagatgacaa gagatggatt cctggaggcg atggacgtgc
     1141 tgggcgagta ttcggaggag aaagcacagg atgtcattga caaggagaac cgggtcgatg
     1201 tctatgacga caagatcacc gcatatctga cgaagctgag cagtgagaat ctgtcctaca
     1261 aggacagcct acaggtgacg tcgctgctgc actgcattac ggattttgag cgtatctccg
     1321 atcactcgat caatgtggtg gagagcgtac agcagatgcg caaggaaaat ctgacgtttt
     1381 ccaggaaggg cgaagaagag atgaatctct acggtgcagc aatccgtgac attctgacaa
     1441 gaaccacgga tgcgtttgtg aacaacgatc aggcactggc acatacggtg gagccgttag
     1501 aggaagtaat tgatgaatta aataaagatg tgaagaaaca tcacatgaag cggctgcgca
     1561 agggcaaatg cagtatggaa ctgggactgc tcctgtcgga tctcgccatg aattatgagc
     1621 gtgtggcaga ccactgttcc aatattgccg tgtacatgat gcagctgaag gatacacagc
     1681 tggaggagca cagttttaca gagcagctgg atgaggcgga gagcgcagag ttcaccagac
     1741 tgctggatga gttcggaagc aagtacgcgc tgtagtctgt atcttcgatc tggtgtgcac
     1801 agggagctgt ggaagagagg agaacaatgg caaccattta tattgtggaa gatgacgtca
     1861 acatccgtga gattgagcgc tatgcgctga agaacagcgg atatgaagtg gaggaattcg
     1921 agagcggtga aagcctgttc aaacggctgg agcgggcggt tccgtccctt ctgctgctag
     1981 acatcatgct tccggatgaa gacggtctgg agattctggg aaagctccgg gcgaataaaa
     2041 atacggcagc cattccggtc attatggtca ccgcaaagac cagtgagctg gacaaggtca
     2101 agggactgga tctcggagcg gatgactata tcacgaagcc gtttggcgtg atggagctga
     2161 tctccagagt aaaagcgctg ctgcgcagga cgcagagtgc ggcggaggag acaaagatct
     2221 gccacggcga gatccggatg gacaacgaca agcatgcggt atttgtcggg aatgagccgt
     2281 gtgagctgac gttcaaggaa tacgaattgt tgaaatatct tatgatcaat gcggggattg
     2341 ttctgtccag agacaagatc atggatcagg tatggggctt tgagtacgag ggcgagagtc
     2401 ggaccgtgga tatgcacatc aagacattgc gccagaagct tggggcggcg ggaagctgca
     2461 tcaagacgat ccgcaatgtg ggatatatga tcgagtagac aaaaacctat gaagaaaaaa
     2521 ttaaattcca aatttatgtt gattgcggca atcgccattc tggtgactgc cgtatgttct
     2581 atcgggctgt tttacagcat tctggtacaa cagatttatg atgacttaaa ggcaaatgca
     2641 catgtgatct cccggctgga tttcgaggat gcgtcgcagg aagtgtgcga tcagctctcc
     2701 gaggatgggc tgcggatcac cctgattgct tcggagggaa ccgtgcttta cgacagcatg
     2761 gaggacgaga cgaagatgga gaatcacaga gggcgtccgg aggtcgagcg ggcgcttgca
     2821 gtcggggaag gccgcgggat gcgcaaatcg acgacttccg cggagcacac attctattat
     2881 gcgatgcgcc tctcagacgg aaatgttctt agaatcggaa aagagagcgg cagcatctac
     2941 catctggcat ggaatatgac gatgcttacg ctgggtgtgg gattgtgtgt attcctggtg
     3001 tgcgcctttt ttgcaaaacg catgacaaag cgctttgtgg agccgatcga aaagatggcg
     3061 gacaacattg tgcttgtcga tgagcgggaa gtatacgagg agatgcgtcc gtttgtgtcg
     3121 atgatcaagc agcagcatat ggatatctta aaccatgcac agatgcggca ggaatttacc
     3181 gcgaacgtat cgcatgaact caagacccca ctcacggcga tctccggcta cgcggaactg
     3241 atcgcaagcg gcatgaccaa cgaaagcgat accgagcatt ttgcaaatga gattcaccgg
     3301 agtgcggaac gtctgcaatc tttgattaac gacatcatca agctctcgga gctggacaac
     3361 agcgacctta agctggagtt tgagccgatc gatctctatg cgctggcgac gacctgcatc
     3421 gatcagatgc agattgcggc gcagaagaac gaagtcacat tgctgcagga aggcgagccg
     3481 gtcacgatca acggcaacaa gacgctcatc gaggaactac tctacaatct gtgcagcaat
     3541 gccgtgcgct ataataagaa gggtggcagt gtgaccgtca ttacgggcat gaagaaccag
     3601 agaccggcac tcacagtgcg cgacaccggc atcggcatcc cgaaggagca gcaggaccgt
     3661 gtctttgagc ggttctaccg tgttgacaag agccgttcca agtcgacggg cggtaccgga
     3721 ctcgggcttg cgatcgtaaa gcacatcgtc gtgcagcacg aagcccagat cgagctctcc
     3781 agcgaggagg gcgtgggcac cgaggtgacg gtaatcttct agcccgcgcg acgcggcaga
     3841 aaccggggcg ctttggtata ttatgacggc ggccggagaa atccgccgga ttctgccgat
     3901 tgccgggagt cgcgattcga tcgcaaaacc ggcggtttct aaccatgctg ttctcacatc
     3961 tgcgatcgtc cgtgtctttc caagctttga acacgctatg gggagtgttc gcacagcaaa
     4021 agacaatttt aaaatttttg tgcagtgctt ggcacaccgg tgagcaaaat ataagaaatt
     4081 gtcgcaataa gcaaagatct ctttgaagtg ataagtaata ttacaggaat accagagaac
     4141 aaaaagttcc agaataaaca gacagcggct tcgcgcggtt atgttttgtt cagaggtgcc
     4201 ataaagggac acacacaaaa gtcctaaaat tgtctgccgg cttgcggaca cagtccgtag
     4261 agtgctccaa acttggaaag acacggatca tcacggatgc cagctgcggc atggttagaa
     4321 ataccggatt ttgcggtcag actttgccgc cgcatgttta tctggaactt ttaagtatct
     4381 atttcccgta atattactta tcaaagcaaa gtgcgatatg cttattgtga cagttccttt
     4441 tattaaatct gtaaagaggt gtgccaagcg ctgcccaaaa gtccactcat tgtctttgct
     4501 gtgcggacac acccgcatag agtgtcaaag cgtggtaaga gacacgaacc cggatgttcc
     4561 agtcggcatg gaagaaaccg cgctattttg ctcaaacgaa tcgtgactcc cggcagccgg
     4621 cagaagcttg cggatttttt ccggacgtca gcatgaattg ctgaaccgcc ccgttctatg
     4681 ccgtgtggag cgcctgccgg gagattgctg tctcggcgtc ggtgtccgcg tcgtgcaggc
     4741 ggtggtactc ttcgataaaa ccgttcattt tgccggcttc ggcggcaagc tctgcgatga
     4801 cgttgttgac gctcttgaag cgctctgcat tgtcggtaca cattgcgttg gtctcctccg
     4861 cggaggcact gatctcctcg cttgtcgaag agagcgtgtt ggtctcatcg accagcatgg
     4921 tgttggtttc ctttaaaatg cgtgtcttct ctgagatggc ggcggcattc tgttcaaggc
     4981 cggtcagacc ggagcggatg ccgctgaaat tttcttcaat atcatgaatc atgctgacct
     5041 gattgccgat tttgtccatg acatgatcca tggaggcaat cgtgtcgccg gcatttttgt
     5101 tcagctttgt gatgatttct tcaatatttt cagtggactg tttggtctgc tcggagagca
     5161 tgcggatctc ctccgcgaca acagcgaacc ccctgcctgc ttcccctgcg cgcgctgctt
     5221 ctatcgtggc gttcaaggcg agaagattgg tctgtgcgga aatctgctgg ataatgctga
     5281 taatttttaa catatcctgc gtgtgtgcct gcagtgcagt catattttcc ttgacgagct
     5341 tattctcaga ttctacttcc gccgtctggg tgacaatatt gctcacaagc gcctgaccgg
     5401 tctctacatt tttaccagaa gactgggaga tgttggtaag tgcgtctgcg atcgacacgg
     5461 tatcggcaat gatgtcctga atccgtccgg tcatggaagc ctgttcctga atgcttgcga
     5521 ctgtgctctc catgcttacg gtaatgtcgc ccattgcctg gctgacgcag ccggttgcct
     5581 ctgtcatctc ctcgatcagc gcatgaatgg actgggtgga ttcattgacg gatgtgccga
     5641 cggtcacgat ggaattcatc atatcctcct gctttttctt gtgggcttcc agttcggcga
     5701 gctcctctgt gttgtgcagg acggccacgc gggacgccag gcaggtgatt gtgttgatga
     5761 caataataat ggagcagatg acaaagatca tattggtttc ggaaagagag ttggttccaa
     5821 gcacatggac agagtaaaca ccgtttaaaa tacagcagac catatttaat acaccggcgc
     5881 agatttccag cttcaggtca aagtacatta atagtgcgac aa
//
LOCUS       DBMDQJ010000047         5874 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5451_length_5874_cov_1.676944, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000047 DBMDQJ010000000
VERSION     DBMDQJ010000047.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5451_length_5874_cov_1.676944"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="ACZO9R_01655"
     CDS             <1..866
                     /locus_tag="ACZO9R_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR0911812.1"
                     /translation="VKMARDAEFQAVIPVRGKILNCLKADYNKIFKNEIITDIIKLLG
                     CGVEVTTKANKDISSFNLDGLRWNKIVICTDADVDGFQIRTLILTMLYRLTPTLIDKG
                     CVFAWFFHYMTVGNDASPELLLTPDQREKMYHKIREYRSTKAIFSMDFQNDAQYVGGC
                     IAGGRNYCHINPKGDVEPCVFIHYSGANIREKSLLECLQQPLFMAYRDGQPFNENMLR
                     PCPMLENPELLQKMVHESGAHSTDVEEAEPVEHLCGKCMEYACQWKETADKLWAEHPY
                     QQKGYTNFKKK"
     gene            915..1892
                     /locus_tag="ACZO9R_01660"
     CDS             915..1892
                     /locus_tag="ACZO9R_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR0911813.1"
                     /translation="MVLVIIGIIVYTFRDSAGPILEQLEKTTPAVIAGICAMTVVYHC
                     VEGVITTLLAKQYNREFTLGMGITNAFLCSFYRVATLGSGAGVAAIVYLGEHGIEYSK
                     SFGMYMLQYAFHKISIAFFSMILFALNWNYMRVHFGEYMWLLLAGYLVTLIITVVLIL
                     FSCSRQFHRMLLWVVDFFNGKTKGRFDPQAAMLHDQCQMLEDASRQLMGEKRLVAGAV
                     GLNLIKFAFWYSIPYLIFLGQGEITLPQTMAVTSLSVMLAAVIPSPAGIGSTEFVFTA
                     LFSGIVGTGLAGSASLLYRFGTFVFPFLVGAVVVIIRRIYLKKTGQAKR"
     gene            2601..4583
                     /locus_tag="ACZO9R_01665"
     CDS             2601..4583
                     /locus_tag="ACZO9R_01665"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR0911814.1"
                     /translation="MKIKLYFDKGNLGRLAVAMIFAAVLFWKIGFSVWALPVFGACYF
                     LFKSLEVELSEKIPWVWTGLMIGASSIFTAYHVQYLLLDAELRAKITQPKMLLNILCC
                     LVVFLAVQVFTNNTGLTCMISHIFLVSLAGINYFVYLFRGNEFIFSDLKSIQTGLSVA
                     GNYEFVMDDRAGYVILISTLYVALIRKLHVSFKKRVPMAIICISLAVLSGVYIGHKTE
                     YIVTETWEQKGSYRNGYILNFVLSIRDCFIAAPGGYSSEAVKALEEQYSGGDSGGKKQ
                     GEKDAASAYADVAADPGEKPTIIVVMSESYADLSVVGDFSTNIDVAPYFHSLKENAIH
                     GYALSSVFGAKTPNSEWEFMTGNSMAFLPSGSVVYQQYISKTPTSIVSNLKNDGYTCV
                     AMHPYYETGWSRNAVYPDLGFDETHFIDDFEQTKLLREYITDRELYEKIIDRYESRAA
                     NEDLFIMSISMQNHGGYTEKYDNFDEKVRLLGLNYPDVNQYLSLVHESDSALEYLISY
                     FQNVDDPVEIVFFGDHQPSLSSSFYPNLNGKGLSGLTEDELEDLYTIPFFIWTNYEST
                     EVELPVTSINYLSTLALERAGIELPAYNQFLADMMETIPAINSRGYYSKSAGGFLHIE
                     EATGEEADWIRNYNILQYNNMFDKKEKSEVFFPYLK"
     gene            4654..5682
                     /locus_tag="ACZO9R_01670"
     CDS             4654..5682
                     /locus_tag="ACZO9R_01670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR0911815.1"
                     /translation="MERVTLGKTGITVNKNGFGALPIQRISTEDAVKLARKAYDAGIT
                     FFDTARFYTDSEEKLGEAFEGMREKVYIATKTAAANADEFWEQLGISLHNLRTDYIDI
                     YQFHNPSFCPKPGDGTGLYEAMLEAKAQGKIHHIGITNHRLAVADEAIESGLYETLQF
                     PFCYLATEKDIELVEKCKQAGMGFIAMKALSGGLINNSAAAYAFEAQYENVLPIWGVQ
                     RERELDEFISYIEHPPVMTEEIAAVIEHDRKELSGEFCRGCGYCMPCPAGIEINNCAR
                     MSLMLRRAPSEAQLTPEMQAKMKKIEDCLHCNKCKSKCPYGLDTPALLAKNYEDYKRV
                     LAGEVSVK"
     gene            5753..>5874
                     /locus_tag="ACZO9R_01675"
     CDS             5753..>5874
                     /locus_tag="ACZO9R_01675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0911816.1"
                     /translation="MKKYETVIFDLDGTLLYTLEDLTKATNYALRRMNMPERTL"
BASE COUNT         1533 a         1259 c         1627 g         1455 t
ORIGIN      
        1 cggtaaaaat ggcacgtgac gcagaattcc aggcggtcat cccggtgcgt ggtaagattc
       61 tgaactgtct gaaagcggac tataataaga tcttcaaaaa tgagattatc acagatatca
      121 ttaaacttct gggctgcggc gttgaagtaa cgaccaaggc aaataaggac atttcctctt
      181 tcaacctgga tggcctgcgc tggaataaaa tcgttatctg taccgatgcg gacgtagacg
      241 gattccagat ccggacactg attctgacaa tgctgtaccg tctgacaccg acgctgatcg
      301 acaagggctg cgtgttcgca tggttcttcc attacatgac agttggaaat gatgcttcac
      361 cggagcttct tcttacaccg gaccagcgtg agaaaatgta tcataagatc agagagtatc
      421 gttccacaaa ggcaatcttc agcatggatt tccagaatga tgcacagtat gttggcggat
      481 gtatcgccgg aggaagaaac tactgccaca tcaacccgaa gggtgatgtt gagccgtgtg
      541 tatttattca ctattcagga gcaaatatcc gtgagaagtc tctgttagag tgtttacagc
      601 agccgctgtt catggcatac agagacggcc agccgttcaa tgagaacatg cttcgtccat
      661 gtccaatgtt agagaatccg gagctgctcc agaagatggt acacgagagc ggtgcacact
      721 caacggatgt tgaggaggcg gagccggtag agcatctttg cggaaaatgt atggagtatg
      781 catgccagtg gaaagagaca gcagataagc tgtgggcaga gcatccgtac cagcagaagg
      841 gctacaccaa ctttaagaaa aaataagggg cgatcgttat cagcaaggcg aaaaaacaga
      901 tcttaaaatg ggcattggta cttgtcatca tcggaatcat tgtttataca ttccgggatt
      961 cagcaggacc gatcttagaa caattagaaa aaacgacacc ggcggtcatt gcaggcatct
     1021 gtgcaatgac tgtcgtgtat cactgtgtcg agggcgtgat taccacgctg cttgcaaaac
     1081 aatataatcg tgaattcaca ttaggcatgg gtattaccaa tgcctttttg tgctcctttt
     1141 accgcgtggc gacacttggc agcggagcag gggttgccgc gattgtctat ctgggtgagc
     1201 acggtataga gtattccaag agcttcggaa tgtatatgct gcagtacgcg tttcataaga
     1261 tcagtatcgc gtttttctca atgattctgt ttgcactgaa ctggaattat atgcgggtac
     1321 attttgggga gtatatgtgg ctgctgctgg cgggatatct tgtaacgctg atcatcaccg
     1381 ttgttttaat tctgttcagc tgttccaggc agtttcaccg catgctgctt tgggtggtgg
     1441 atttcttcaa tggaaagacg aaagggcggt ttgatccgca ggcagcaatg cttcatgacc
     1501 agtgccagat gttggaggat gcatccagac agctgatggg agagaagcgg ctggttgcgg
     1561 gtgcagtggg attgaacctc atcaagttcg cattctggta ttccatcccg tatctgatct
     1621 ttctggggca gggggagatt acgctcccgc agacgatggc ggtcacatcg ctttctgtca
     1681 tgcttgcggc agtcattccg tcaccggcgg gaatcggttc gaccgagttt gtctttaccg
     1741 cacttttttc cggtattgtc ggaaccggac tggcaggctc cgcgtcgctt ctgtaccgtt
     1801 tcggtacttt cgtattcccg tttctggtgg gggcggttgt ggtcatcata cgccgcatct
     1861 acctcaaaaa aacgggacaa gccaaacgat agcgccagct gtgaaatgag gctggtacac
     1921 agggcggaga atagactggc agtgatacga taacagtaag tacagcagag ttccagaata
     1981 gaaacagtgc gtaggaattc ggatcgcaaa atacggaatt tctaactctg ccgcttttta
     2041 catctgtgat gatccgtgtt tttccaagtt ttgagcactc cgcaggtgtg tccgcaggcc
     2101 gacagacaat ttaaggactt ttgtgtgttc cttacatcac caatgaacaa aacataaccg
     2161 cgcgaagccg atgtctgaat tggcatctgc cttattttca atagcagatg ccaacgagtt
     2221 tcggttcgcg cggttttata ttgtctgtga agcggtgatg ttaggaacac taaaaagtcc
     2281 gctcattgtc tgcggcgtgt cggacacatc ccgcggattg tcaaaacgtg gaaagacacg
     2341 gaattggata taaaaagcgg cagagaagaa attctcgcat tttgttccaa cgaatatcct
     2401 atgcactgtt tctatctgga actttattgc atcctacatt ttattgtgac cggcaatcta
     2461 ttctccgccc tgtgtgcgag cctcattttg cgcttctttt gaaaagttta aggtttggga
     2521 aattgacaaa cgcacaagtc atgataaaat taacgttggc atgcaaaaaa tatgtaaaga
     2581 aagcgtaaat aaggaaacat atgaaaataa aattatattt tgacaagggt aatttgggca
     2641 gacttgcggt ggcgatgatc tttgccgcgg tattgttctg gaaaatcggc ttttcggtgt
     2701 gggcgctgcc ggtattcgga gcgtgctact tcctatttaa gagtctggag gtggaactgt
     2761 cagagaagat cccgtgggtc tggaccggac tgatgatcgg cgccagcagt attttcacgg
     2821 cgtatcatgt gcagtatctg ctgctggatg cagaacttcg cgccaagatc acacagccga
     2881 agatgctgct caatatcttg tgctgtctgg tggtatttct ggcggtacag gtttttacca
     2941 ataatacggg acttacctgc atgatttccc acatttttct ggtgtcattg gcgggaatca
     3001 actattttgt atatctgttc cgcgggaacg aatttatttt cagcgacctg aaatccatcc
     3061 agaccggact ttcggtggcg ggcaattatg agttcgtgat ggatgaccgg gcgggctatg
     3121 tgattctgat ttccacgctc tatgtggcgc tgatccgcaa gcttcacgtt tcctttaaaa
     3181 agagagtgcc gatggcgatc atctgtatct ctcttgccgt gctgagcggc gtgtatatcg
     3241 gtcataagac ggagtacata gtgacagaga cctgggagca gaaaggaagc taccgcaacg
     3301 gatatattct gaattttgtg ctgagtataa gagactgttt tatcgctgcg cccggcgggt
     3361 attccagtga ggcagtcaaa gcgctggaag agcagtattc cggcggggat agcggcggga
     3421 agaagcaggg agagaaagac gctgcctcgg catatgcgga tgtggcggct gatccgggag
     3481 aaaagccgac cattatcgtg gtcatgagcg agtcgtatgc ggatctttcg gtggtgggag
     3541 atttttccac gaatattgat gtggcgcctt atttccactc cctgaaggag aatgccattc
     3601 acggatatgc gctgtcctcc gtgttcggtg cgaagacgcc gaattcggag tgggagttta
     3661 tgaccgggaa ctccatggca tttctgccga gcggttccgt ggtataccag cagtatattt
     3721 ccaagacgcc gacctcgatt gtgtccaact taaagaacga cggttatacc tgtgtggcaa
     3781 tgcatccgta ctatgagacc ggatggagcc gcaatgcagt gtatccggat ctcgggtttg
     3841 atgagacaca ttttatcgac gattttgaac agacgaagct tctgcgggaa tatatcaccg
     3901 accgggaact gtatgagaaa attattgacc gctatgagag ccgtgcggcg aatgaggatc
     3961 tgttcatcat gagcatttcc atgcagaacc atggcggtta tacggaaaag tacgataatt
     4021 ttgatgagaa ggtacgcctg ctgggattaa actatccgga cgtcaatcag tatctctctc
     4081 tggtgcacga gagcgattca gcgttggagt atctgatctc ctattttcaa aatgtggatg
     4141 atccggtgga gatcgtattt ttcggcgatc accagccaag cctttcgtcc agtttttatc
     4201 cgaacttaaa cggcaaggga ctttcgggac tgaccgagga tgaactggaa gatctgtata
     4261 cgataccgtt tttcatatgg acgaactatg agagcacgga ggtggaactg ccggtcacca
     4321 gcatcaatta tctctcgacg ctggcgctgg agcgggcagg aatagaactt ccggcgtaca
     4381 atcagttcct ggcggatatg atggagacca ttccggcaat caattccaga ggctattatt
     4441 ccaagtccgc cggcggattt ttgcacatcg aggaagcgac gggtgaggaa gcggactgga
     4501 tccggaacta taatattttg cagtacaaca acatgtttga caaaaaggaa aagagtgaag
     4561 tattctttcc ttatctgaag tagtattaag aaagcaacag agatacagct ttttagcggg
     4621 caatgtgtat aagaaaaatg tggaggcgaa aaaatggaga gagtgacact tggaaaaaca
     4681 gggattaccg tcaataagaa cgggtttggc gcacttccga tccagcggat cagcacggag
     4741 gacgccgtga agctggcgcg aaaggcatat gatgccggca tcactttttt tgataccgcg
     4801 cggttctaca cggacagcga ggagaagctt ggagaagcgt ttgagggcat gcgggaaaaa
     4861 gtctatattg cgacgaagac ggcggctgcc aacgcggacg aattctggga gcagcttggc
     4921 atttccctgc acaatctgcg cacggattac attgacatct atcagttcca caatccgtcc
     4981 ttctgcccga agccgggcga cggaaccgga ctttacgagg cgatgctgga agcgaaggca
     5041 cagggaaaaa tccaccacat cggtatcacg aaccaccggc ttgccgtggc agatgaggcg
     5101 atcgagtcag gactttatga gacgcttcag ttcccgttct gctatctggc gacagagaag
     5161 gacatcgagc tggtggagaa gtgtaagcag gcgggaatgg gatttattgc aatgaaagcg
     5221 ctctcgggag gattgatcaa caattcggct gcagcgtacg cgtttgaggc acagtatgag
     5281 aatgtgctgc caatctgggg cgtgcagaga gagcgggaac tggacgagtt catttcctac
     5341 attgaacatc cgccggtcat gacggaggag atcgcagcgg tgattgagca tgacagaaaa
     5401 gaactctcgg gcgaattctg ccgtggctgc ggttactgca tgccatgtcc tgccggaata
     5461 gagatcaaca actgtgcgag aatgtccctg atgctgcgcc gcgcgccgtc ggaggcacag
     5521 ctgacaccgg agatgcaggc gaagatgaaa aaaatcgagg actgcctgca ctgcaacaag
     5581 tgcaagagca aatgtccgta cggactggac acaccggcac tgcttgcgaa gaattacgaa
     5641 gattacaagc gtgtactggc aggagaagtc agcgtaaaat aagggatgtg cagacatgca
     5701 gaacgacatg caatgcgcgt aatgcatgcg tgagacgaaa aggagtgaga gaatgaagaa
     5761 atatgagacc gtcatttttg atctggatgg aacacttttg tatacactgg aggatctgac
     5821 aaaggcgacg aactatgcgc tgcgcaggat gaatatgccg gagcgcacgc tgga
//
LOCUS       DBMDQJ010000048         5850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5473_length_5850_cov_1.670551, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000048 DBMDQJ010000000
VERSION     DBMDQJ010000048.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5473_length_5850_cov_1.670551"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            336..467
                     /locus_tag="ACZO9R_01680"
     CDS             336..467
                     /locus_tag="ACZO9R_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911817.1"
                     /translation="MECSKLGKTRIIEDANIRLVRNATFFEPNIHLNFQLKSHGVIF"
     gene            complement(530..1831)
                     /locus_tag="ACZO9R_01685"
     CDS             complement(530..1831)
                     /locus_tag="ACZO9R_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HIR0911818.1"
                     /translation="MNIVLTTIILFVVFGVLLFMGVPISVSIIVSSIVTAMSTLSWDQ
                     ITFITMQKMNSGIESFSLLAVPLFILAGNIMNNGGIAKRLVNFAQLFVGKLPGNLAQA
                     NILGNMLFGALSGSSVAAASAMGGCISPIEKEQEYDPAYSAAANIASAPTGLLIPPTS
                     AFIVYSTVAGGVSISTLFMAGYVPGILMGLGCMVVAFIYAKKMGIKATGRDKSISVAK
                     TIWDAIPSLLLIIIVIGGIVSGIFTATEGAGIAVLYCLILSIIYKSIDFKGFMEILLN
                     SAKTSGIILFLISASSAMSFVMAYSGIPAAISSALLSVSTNKYVIFILMNVILLIIGM
                     FMDITPAILIFTPIFLPIAQSFGMSDIQFGVMLIFNMCLGNITPPVGSVLFVGCGIGH
                     VSIEEVTPKLIPYFLVLIVMLLLVTFVPGLSLGVPSLMGLV"
     gene            complement(1843..2334)
                     /locus_tag="ACZO9R_01690"
     CDS             complement(1843..2334)
                     /locus_tag="ACZO9R_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HIR0911819.1"
                     /translation="MAKTLGTIKKYMNKVLAACCAVLLTFMTFLVLWQVFTRYVLNSP
                     AAFTEELVKYSLIWTGFIGAAYAFSTREHMALTLVRDKLTGRPHQILVIFIDALILLL
                     AIFVITIGGYKLAVSARAEFSALLGVPRTLVYSIAPISGIFIIIAQIINLYEDITGNK
                     VEE"
     gene            complement(2338..3381)
                     /locus_tag="ACZO9R_01695"
     CDS             complement(2338..3381)
                     /locus_tag="ACZO9R_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HIR0911820.1"
                     /translation="MKKNIALILAAALVLPTVIAAGGCGANSGVSSSAEATAENPMVL
                     TLAHGLSETHTVHIAMTQFADEVKEKTDGRIIINIFPNGQLGSETENLEQLQAGVIAM
                     TKVSAPGLATYNEAYNTFGLPYIFNDTEDFYDIMDSEDMQDFFLSSEEDGFVTITYFT
                     SGARSFYTKDKAIRVPEDLKGLKIRVQDMKSQTDMLTALGGTPVAMSYGDVYTALSTG
                     IIDGTENNETALTTGKHGEVCKVYSVDQHAMIPDVMVMSAKVWNNIAPEDQEIILQCA
                     HDATEWHKEAWDQAVDEAITEAEDMGVEFVYDVDKEAFREATAEMIDEYREEYPGVAD
                     LLDRIDGIREEEE"
     gene            complement(3421..>5850)
                     /locus_tag="ACZO9R_01700"
     CDS             complement(3421..>5850)
                     /locus_tag="ACZO9R_01700"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HIR0911821.1"
                     /translation="WFLTDDIIRIRAGFDGDWDEASYSLVTTAWESRTDELMKDYRKR
                     ITVAESTLVDGETRAVITGKKLRVEVEKDPFRICVYDAEGTMLHADIPELAYREDSNR
                     RRIHTSQIEDDDYFYGFGEKGGEINKAEKYMNMAPGDAMGYNAKETDSLYKHIPFYIK
                     LQRGTKKAVGYFYHSTAECDFNMGREKRNYWHRYSSFRADAGDVDLFLIAGPSIGEVI
                     ERYTDLTGKSVLLPKSAFGYLGSSMYYPELPENCDDAILEFIDTTKEEGIPVDGFQLS
                     SGYCAVETEQGIKRCSFTWNYKRFKDPADWFAKMKQEGIVVSPNVKPGMLLVHPLLEE
                     MKEKKMFVLDSTTDEPGIGTWWGGTGIFVDFTKEENRKNWKEYLKQGLIQYGCESVWN
                     DNCEYDSMVDKDARVYFEGKGSTIGTMKPVMSNLMCQLSNEAVTEYNPSVRPFSVCRS
                     GHAGIQRYAQVWAGDNLTCWDTLKYNIATILGMGLSGVANHGCDVGGFYGVSPEPELF
                     VRWVQNGIFMPRFSIHSTNTDNTVTEPWMYTDKKQYIRDAIKFRYRMIPYLYSLESRA
                     HETGLPIMEPMFMAFQNDPKTYNEGVDFMWGDSLLVANVVEKGAKTRSIYLPQTGDEN
                     VRFYDFYTREEYAPGQTIEVPVDISSIPLYIKSGAIIPMAENQIYNLMHETVTGLHIL
                     MAPDVDSEFTVYDDDGKTNEYQNGAYLKTNIKVTAGVRTCVDFTYEGNYENTVDTMYL
                     DVVHREKAPFYVQIDGVEVPHFLHRRKFEEAQTGWYYSQSLKSVQVKYPNPKKDHQVM
                     ISFEVFDMIGM"
BASE COUNT         1490 a         1521 c         1305 g         1534 t
ORIGIN      
        1 atgtggaaat gcagatggaa tattcgttgg agaaaaatgt gtgcatttct tcctgctcaa
       61 tgtttgcatc gaattccgtg tctcttccac gtttggacac tcccggagtg tgtccgacaa
      121 atcagagaca atgggcgaac tttctagtgg cggctaatgc cgctgcttca tagacattat
      181 aaaatcgcgc gaaccgaaac tcgctgccat ctgctattga aaataaggca gatggcaact
      241 cagacatcgg cttcgcgcga ttatgtttta ttcagcagcg gcaaaagcgc cacacgaaag
      301 ttcttaaatt gtctcatgat ttgcggacac agtccgtgga gtgctccaaa cttggaaaga
      361 cacggatcat cgaggatgca aacattcggc tggttagaaa tgccacattt tttgaaccga
      421 atatccatct gaatttccag ctgaaatccc acggtgtgat cttctaataa tattacttat
      481 caacccaaaa gaggtctatg cttattgcgg caattccaca ggtctatcct taaactaatc
      541 ccatgaggct cggaaccccg agggagagtc ccggtacaaa ggttaccaga agaagcatca
      601 caatcagtac caggaaatat ggaataagct tcggagttac ttcctcaatc gatacatgtc
      661 cgataccgca tccgacaaaa agtacggagc ctaccggcgg tgtgatattt ccaaggcaca
      721 tattgaaaat taacattacg ccaaactgga tgtcgctcat gccaaagctc tgtgcaatcg
      781 gcaggaagat cggtgtaaaa atcaagattg ccggtgtgat gtccatgaac ataccgatga
      841 tcagcaggat aacgttcatc agaatgaaga tgacatattt gtttgtggat accgagagca
      901 gcgcgctgct gattgctgcc gggattccgg aataagccat cacgaatgac atcgcggaag
      961 aagcagaaat caaaaacagg atgattccgg aagtcttggc agagttcagg agaatctcca
     1021 taaatccctt gaaatcgata ctcttgtaga taatggacag gatgaggcag taaagaactg
     1081 caatacctgc gccttccgtt gccgtaaaga taccggatac gataccgccg ataacgataa
     1141 tgatcagcag cagggacgga atggcatccc agatcgtctt tgcaacactg atggatttgt
     1201 cacggccggt cgccttgatg cccatttttt tcgcatagat gaatgcaacc accatacagc
     1261 caagacccat gaggataccc ggaacatatc cagccataaa cagcgtggaa atggatacgc
     1321 cgccggctac ggtagagtaa acgatgaatg cagaggtcgg aggaatcaga agtccggtcg
     1381 gtgcggaagc aatgttggcc gccgccgagt aagccggatc atactcctgc tctttttcaa
     1441 tcgggctgat acatccaccc attgcagatg cagccgctac ggaagaaccg gagagcgctc
     1501 caaagagcat gtttccaaga atattggcct gtgccaggtt tcccggaagt tttcccacga
     1561 aaagctgtgc aaaattgaca agcctctttg cgataccgcc attgttcatg atgttaccgg
     1621 caaggatgaa cagcggaacc gccagcaggg aaaaactttc gataccggaa ttcatcttct
     1681 gcatggtaat aaacgtaatc tgatcccagg aaagggtaga cattgcggtt acgatggaag
     1741 atacaataat acttacagaa atcggaacac ccatgaacag cagtacgccg aaaacgacaa
     1801 acaaaatgat agttgttagt acgatattca ttttttccct ccttattctt ctactttgtt
     1861 tccggtaatg tcttcgtaga gattaataat ctgtgcgatg atgataaaga tgccggaaat
     1921 cggtgcgatg gaatatacca gcgttctcgg aactcccaga agtgcggaga attccgccct
     1981 tgcagacact gccagcttat aaccgccaat cgtgattaca aagattgcaa ggagtagaat
     2041 cagcgcatca atgaagataa ccagaatctg atgcggtctt ccggtcagtt tatctcttac
     2101 cagggtcagt gccatatgct ctctggtgga aaatgcatag gccgcgccga taaagccggt
     2161 ccagatcagc gagtacttta ccagttcctc ggtaaatgcc gccgggctgt tgaggacgta
     2221 tctggtaaat acctgccaca ataccaggaa ggtcatgaac gtcagaagga ctgcgcagca
     2281 agccgcgaga actttgttca tatatttctt aatcgtgcct aaggtcttag ccatttttta
     2341 ctcctcctct tctctgattc catcaattct atccagcaaa tccgcaactc ccggatattc
     2401 ttcgcggtat tcatcgatca tttccgccgt cgcttcacgg aaagcttcct tatctacatc
     2461 gtagacaaat tccacaccca tatcttccgc ttccgtaatt gcttcatcca cagcctgatc
     2521 ccatgcttcc ttgtgccatt cggtcgcatc gtgcgcacat tgcaggatga tttcctgatc
     2581 ctccggagca atattgttcc atacttttgc tgacatgacc atcacgtcag gaatcatcgc
     2641 gtgctggtca acagagtaca ccttgcagac ctcgccgtgc ttacctgtgg tcaatgccgt
     2701 ctcattgttc tcggtaccat cgataatgcc ggtggaaagt gccgtataaa catcaccata
     2761 ggacattgcc accggagttc cgccaagcgc tgtcagcata tccgtctgcg acttcatatc
     2821 ctgtacacgg atttttaatc cctttaaatc ttccggcaca cggattgcct tgtctttggt
     2881 gtagaaggaa cgggcgccgg acgtaaagta ggtaatcgtc acaaatccgt cttcctcgga
     2941 ggaaaggaag aaatcctgca tgtcctcaga atccataatg tcgtagaagt cttcggtatc
     3001 attgaaaatg tacggaaggc cgaacgtatt gtaagcttcg ttataggtgg caagacccgg
     3061 tgcggaaacc ttggtcattg cgattacgcc tgcctgcaac tgctctaagt tctctgtctc
     3121 agaaccaagc tgtccgttcg ggaaaatgtt gataatgatg cgtccgtcgg tcttctcttt
     3181 tacctcatcc gcaaactgcg tcatggcaat atgtacggtg tgcgtctcgg aaagaccgtg
     3241 tgccagagtc aataccatcg gattctctgc cgttgcctct gcactgctgg aaactccgga
     3301 gtttgcgcca cagccgcctg ccgcaattac ggtcggcaaa acgagcgctg ccgccaaaat
     3361 gagggcgatg ttctttttca ttgcttttgt tctccttttc cttttttatt ttaatttaaa
     3421 ttacattcca atcatatcga atacctcaaa ggaaatcatc acctgatgat cctttttcgg
     3481 attcgggtac ttcacctgta ccgactttaa gctctggctg taataccagc cggtctgcgc
     3541 ttcttcaaat ttgcgtctgt gaagaaaatg cggaacttcc acaccgtcaa tctgaacata
     3601 gaacggcgct ttttccctgt gtaccacatc cagatacatc gtgtccacgg tattctcata
     3661 atttccttca taagtgaagt ccacacaggt tctgactcct gccgtcactt taatattggt
     3721 cttaagatat gccccgttct ggtactcatt ggtctttccg tcatcgtcat atacggtaaa
     3781 ttcagaatct acgtccggcg ccataagaat atgaagtccg gttaccgtct catgcatcag
     3841 gttgtagatc tgattctccg ccatcggaat aatcgcaccg ctcttgatgt aaagcggaat
     3901 ggaactgata tcgaccggca cctcgattgt ctgtcccggc gcatactctt ctctggtata
     3961 aaaatcatag aagcgaacgt tctcatcccc cgtctgcggc aggtaaatgc ttctggtctt
     4021 tgctcctttt tccacaacat tagctaccag gagtgaatcg ccccacataa aatctacgcc
     4081 ttcattgtag gtcttcgggt cattctgaaa tgccataaac atcggctcca taatcggaag
     4141 accggtctca tgcgctctgc tctccagaga atacagatac ggaatcatgc ggtagcggaa
     4201 cttgattgcg tcccggatat actgcttctt gtccgtgtac atccacggtt ccgttaccgt
     4261 attgtcggtg ttggtcgagt ggatggaaaa tctcggcata aagataccgt tctgcaccca
     4321 gcggacgaaa agctccggtt ccggagaaac accatagaaa cctccgacat cgcagccgtg
     4381 gtttgcgact ccggagagtc ccattccgag aatggttgcg atattgtatt ttaacgtatc
     4441 ccagcaggtc aggttatcgc ctgcccacac ctgtgcgtat cgctggattc cggcatgtcc
     4501 ggaccggcac acggaaaacg ggcgaaccga tggattgtac tccgtcaccg cttcattgga
     4561 cagctggcac atcaggttgc tcataaccgg cttcatcgta ccaatcgtgc tgccttttcc
     4621 ctcaaaatat actctggcat ccttgtctac catggaatcg tattcacagt tatcgttcca
     4681 tacagactcg cagccatact ggatcagccc ctgcttcaga tactccttcc agttcttgcg
     4741 gttttcttct ttggtaaagt cgacgaaaat tccggttccg ccccaccagg taccaattcc
     4801 cggctcatcc gttgtgctgt cgaggacgaa catcttcttt tccttcatct cctccaggag
     4861 tggatgaacg agaagcattc ccggctttac attcggggag acaacgatgc cctcctgctt
     4921 catctttgca aaccagtccg ccggatcctt gaatctttta taattccagg taaagctgca
     4981 tctcttgatg ccttgctctg tctcgacggc acagtatccg gaagaaagct ggaaaccatc
     5041 caccggaatg ccctcttcct tggtggtgtc aatgaattcc agaattgcat cgtcacagtt
     5101 ttccggcagt tccggatagt acattgacga tcccagatat ccgaatgcag acttcggaag
     5161 aagtacggac tttcctgtca gatcggtgta gcgctcaatg acctccccga tcgacggtcc
     5221 cgcgatcaga aataaatcca catcgcctgc atccgcacgg aaggaagaat aacggtgcca
     5281 gtaattccgc ttctcgcgtc ccatattgaa gtcacattct gccgtgctgt gataaaaata
     5341 tccgacggct tttttcgtgc cgcgctgcag cttaatgtag aacggaatat gcttgtacag
     5401 agaatcggtc tccttcgcat tgtatcccat cgcatctccg ggagccatgt tcatatattt
     5461 ctctgcttta ttgatctcgc cgcctttttc accaaatccg taaaaataat cgtcatcctc
     5521 gatctggctg gtatgaatgc ggcgcctgtt agaatcctca cggtacgcaa gctccggaat
     5581 atccgcatga agcatggttc cttctgcatc atagacacag atgcggaacg gatctttttc
     5641 cacttccaca cggagctttt ttccggtaat cactgccctg gtctcaccat ccaccagtgt
     5701 agattccgcc acggtaatcc gcttccggta atccttcatc agttcatctg tgcgggattc
     5761 ccacgcggtt gttaccagac tgtaggacgc ttcatcccag tctccgtcaa agcccgcccg
     5821 gatacggatg atatcatccg taaggaacca
//
LOCUS       DBMDQJ010000049         5819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5495_length_5819_cov_1.517664, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000049 DBMDQJ010000000
VERSION     DBMDQJ010000049.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5495_length_5819_cov_1.517664"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(148..1479)
                     /locus_tag="ACZO9R_01705"
     CDS             complement(148..1479)
                     /locus_tag="ACZO9R_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6382 domain-containing protein"
                     /protein_id="HIR0911822.1"
                     /translation="MKIQYIRKLTASYMVLAQCEELQEWEKKMIAHAPKGNIVFAECV
                     QENGEKYLWYNITGKQALDAVLETETFSYDMLCNVLNGIYEAVQQLEGMLLPAQSILL
                     SAESIFVDYRTRQISFCYYPGNPMPLPEAFRTFMEYLLTRLDHSDERAVKAAYDIYGQ
                     SSAGGVELKELKSLLLMPYEKDEAKTEPEGLDDTEPEGSKAYATVGQKEQDGKRDASI
                     WKQIFRGESTVQELLAAVWEKIWKTTGTAAGRPAGGAAGRLAGGAAGRPAGEKAGRLT
                     GGAAGRPAGEKAGRVTGKAAASGDKRRMGRWKIGGNMKNMQKEPEAFVFEPEEEEHKS
                     ANPTVLLAGISQKPAGILRYEGTGGYSDLVIEKDNYVIGSAAECDGRIPSATVSRRHA
                     KISKKGAVFFIEDLNSSNGTRVGGELLNYKTGMSLQKNEVVMFADEKFRFI"
     gene            complement(1460..1681)
                     /locus_tag="ACZO9R_01710"
     CDS             complement(1460..1681)
                     /locus_tag="ACZO9R_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR0911823.1"
                     /translation="MNGWRGSLTIEAALVVPLVLALFAFAMKSGIQMYTECRDTALAI
                     GQEQEIEAVKMFYRWQEAGELFQNEDTVY"
     gene            complement(1706..2134)
                     /locus_tag="ACZO9R_01715"
     CDS             complement(1706..2134)
                     /locus_tag="ACZO9R_01715"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR0911824.1"
                     /translation="MQNCVGEVLLLGIFAVTAYRDWKEQMICVYVPLAAGIAGLVLHL
                     MLPTQKLTDLLLGAGIGAVLLLAAWVSREEIGYGDGAMLMASGIFLGFWRNVELFLTA
                     LMLIVPAALFGLIVKHRRKDYRMPFLPFLFAAYVFQLGWR"
     gene            complement(2169..2876)
                     /locus_tag="ACZO9R_01720"
     CDS             complement(2169..2876)
                     /locus_tag="ACZO9R_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR0911825.1"
                     /translation="MMPVLACFFVSVLFFFRIMQIQLEVQRALDDTGRKLAVCATTVS
                     SDGAALPGMAEALLLREMADRTEAETYITGGKFGISLIGSDFSGEEITLRAAYHIRLP
                     IRIFWLWDFAMEQRADCRKWTGWSAAEGSDEDQWVYVTQTGTVYHREYSCTHLELSIR
                     SVAEAEIAALRNENGEAYRKCMQCRYAENAFGRLYITNQGDCYHKDLGCSGIKRTVRM
                     IRLSEVGTRRPCSRCGQ"
     gene            complement(3055..4527)
                     /locus_tag="ACZO9R_01725"
     CDS             complement(3055..4527)
                     /locus_tag="ACZO9R_01725"
                     /inference="COORDINATES: protein motif:HMM:NF039199.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5702 domain-containing protein"
                     /protein_id="HIR0911826.1"
                     /translation="MRKRASITVFSALAFALVASFLMALLEAGRVYQIQTYADMKSTL
                     ALESVCAEYQPVLWEEFHLLGLDGAYGGDIFSMDHVTAVLRERLDRNLDVDGAGGSIL
                     QVTLAGAEPVAYRLMTDQEGSVFLHCVAAYMKQNMAMETIRALQERYGEHEQVEQSGQ
                     GEESIEDARTAIEEAKSERREQAESDGTEAVIPELPPEQENPLEVVHAIKQNALLGMT
                     LGDFGAVSTRQTDLGDSIERRQKQTGNMEIQVSDWYDRILALEYMDQYFGDYLGESED
                     HALAYELEYVLAGKGSDKENLESVIKRLLFVREAANITHILGDGDKRAKTLGMAEALA
                     GFTGNPAVVRLVQTGVIAAWAYVESILDIRALLAGDKIALIKTETQWTAQFGSLAAAF
                     EDGKKAKNCENGVSYQGYLKGFLYTVSSERLAYRMMDIMERTVRLHPMYADCRMDHVL
                     CGISYRMDYEWQPLFSGLMVHTGRDLSGLCYQTQRTFSYD"
     gene            complement(4530..4697)
                     /locus_tag="ACZO9R_01730"
     CDS             complement(4530..4697)
                     /locus_tag="ACZO9R_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp1 family type IVb pilin"
                     /protein_id="HIR0911827.1"
                     /translation="MRGIREFIREEDGVGVVELILILVVLIGLVLIFKDQLTSLVNDI
                     FDTITKKAGKV"
     gene            complement(4713..>5819)
                     /locus_tag="ACZO9R_01735"
     CDS             complement(4713..>5819)
                     /locus_tag="ACZO9R_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012694.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR0911828.1"
                     /translation="TWVGFGQLLLSKLRMYVDGAKKAKAYKVHVPEQELTKQEEQAYL
                     RAAEEELIKEFPGDNESVNCIRDAVVIREEYQEGHVEAEWSFDNYRLIESRTGRIVGE
                     PDEEGELVNVQVTLTCGKSRCVQEFYICVYPRLLDEEERFRKGISEKIARQQSEAGAD
                     RLFLPDEVEGHTIHWEQRRDQRPEKILILGVILACFVPVVSMSRKQEEQKKRKMLLEL
                     EYPDLVSKLALLLGAGMTLQGAFRKIAGAYGQKRTQNKTAEMPAYEEMLVACRQMESG
                     VGEQRAYEHFGERCGVASYRKLAGILTQNLQKGTRGITVLLEQEAQNAFEERKHAAKR
                     YGEEAGTKLLFPMMLMLGIVMVILLVPAVLAFQI"
BASE COUNT         1251 a         1924 c         1092 g         1552 t
ORIGIN      
        1 gttttacttt acaaaaacga tatccttcct gagaaaatat ctccatccat tcgtttctca
       61 ttcgctgtcc actccatttc atagcctttc atggatatta tagagatttc cagaggtaga
      121 agcaatgata tttctccgat gtttttctta aataaatcta aacttttcat ctgcaaacat
      181 cacgacctca tttttctgca gactcattcc tgttttataa ttcagcaatt ctccacccac
      241 tctggtgccg ttcgacgaat tgagatcctc tataaaaaag acagctccct ttttgctgat
      301 ctttgcatgc ctcctgctca cggtcgcact cggaatccgg ccatcacact ccgctgcact
      361 cccgattaca tagttgtctt tctctatcac cagatccgag tatccgccgg ttccttcgta
      421 gcgcaggatt cccgccggtt tctgcgagat tcccgccaaa agcaccgtgg gatttgcgga
      481 tttatgttcc tcttcctccg gttcaaagac aaacgcctcc ggctccttct gcatattttt
      541 catattcccg ccgatcttcc atctgcccat tcttcttttg tccccggatg ctgccgcttt
      601 gcctgtcact ctgcctgcct tttcacctgc cggtctgccc gcagctccgc ctgtcagtct
      661 gcccgccttt tcacccgccg gtctgcctgc agctccgcct gccagtctgc ctgcagctcc
      721 gcccgccggt ctgcctgcag ctgtgcctgt cgttttccat attttctccc acactgccgc
      781 aagcagttcc tgcaccgtgc tctctccgcg aaaaatctgc ttccatatgg aagcatcccg
      841 cttcccgtcc tgttccttct gtccgacagt ggcatatgct ttcgaccctt ctggttctgt
      901 gtcatccaga ccctccggct ctgtcttcgc ttcatccttt tcatacggca ttaaaagcag
      961 cgatttcagc tcctttaact ccactccccc tgcactgctc tgtccgtaaa tatcatatgc
     1021 cgctttgacc gcacgctcat ccgaatgatc cagtcgtgtc agcagatatt ccatgaacgt
     1081 ccggaaggct tccggcagcg gcattggatt ccccggatag tagcagaaag aaatctgtct
     1141 ggtccggtaa tctacgaaaa tactttccgc cgacaacaga atactctgtg ccggcagcag
     1201 cattccctct aactgctgca ctgcctcata aattccgttg agtacattgc acaacatatc
     1261 ataggaaaac gtctccgtct ccaacaccgc atccagcgcc tgttttcctg taatgttata
     1321 ccacagatac ttttctccgt tctcctgcac acattccgca aacacaatat tccccttcgg
     1381 agcatgagca atcatctttt tttcccattc ctgcaattct tcacactgcg cgagcaccat
     1441 gtagcttgcc gtcagttttc taatatactg tatcttcatt ttgaaataat tcccccgctt
     1501 cctgccagcg gtaaaacatc tttaccgcct ctatttcctg ctcctgccct atcgccagcg
     1561 cggtgtccct gcactctgta tacatctgaa taccgctctt catggcgaat gcaaacaacg
     1621 ccagcacgag cggcaccaca agcgccgcct ctatggtcag acttcctctc catccgttca
     1681 taaattctcc atttcgtccc gttttctacc gccatcccag ctgaaataca tacgccgcaa
     1741 aaaggaatgg aagaaacggc atccggtaat cctttcttct atgcttcaca atgagtccaa
     1801 acaacgccgc cggaacaatc aacataagcg ccgttaaaaa caactccaca ttccgccaga
     1861 atcccagaaa aataccgctt gccatcagca ttgcgccatc cccgtagcca atctcctccc
     1921 tgctgaccca agccgcaagc aaaagcaccg cgccgattcc tgcacccaac agcagatccg
     1981 tcagcttctg tgtcgggagc atcaggtgaa gaaccagtcc tgcaattccc gccgcaagag
     2041 gcacatacac gcatatcatc tgttccttcc agtcgcggta tgccgtgacc gcaaatattc
     2101 caagcagcaa cacctcaccc acacaattct gcattgtctt tcttctcctc tcatccatct
     2161 ctctcccgct actgcccgca ccggctgcac ggtctgcgtg ttcccacctc ggacagccgt
     2221 atcatgcgga cggttcgctt gatgccgctg cagccaagat ctttgtggta gcagtcgccc
     2281 tggttcgtaa tatagagccg tccgaatgca ttttccgcat accggcactg catgcatttt
     2341 ctatatgctt ccccgttctc attgcgaagt gccgcaattt ccgcctccgc cacagaccgg
     2401 atcgacagct ccagatgcgt gcagctatac tccctgtgat agacggttcc tgtctgtgtg
     2461 acatacaccc actggtcttc atcacttccc tctgcagcac tccatcctgt ccatttcctg
     2521 caatctgccc gctgttccat cgcaaaatcc cacaaccaga agatccggat gggcagccgg
     2581 atatgatacg ccgcccgcag tgtgatctcc tccccggaga aatcggatcc aatcaggctg
     2641 attccaaact ttcctcctgt gatgtaagtt tctgcttccg tgcggtccgc catctctctc
     2701 aaaagcaaag cctctgccat tccagggagc gccgccccgt cggatgacac agttgtcgcg
     2761 cagactgcca gcttccgtcc cgtatcatcc aatgccctct gcacctcaag ctgaatctgc
     2821 atgatgcgga agaaaaagag aacactcaca aaaaagcatg ccagcaccgg catcacgact
     2881 gctgcctcca gtgtaaatac tgcagacaga cggaggcatt gcggtgtccc ttttcgcgcg
     2941 atattttctg aaccagatat ggaattttgt tcggggagag atttgtcctt tcttattatt
     3001 tttatgattc gccttgcttc tacctcataa agagacaccg caacacctcc ctcctcaatc
     3061 atacgaaaac gttcgctgtg tctgatagca tagaccggac agatctctcc ctgtatgtac
     3121 catcagcccg gaaaacaacg gctgccactc ataatccatg cggtaactta tgccacacag
     3181 cacatgatcc atgcggcagt ctgcatacat tggatgcagg cgtactgtcc gttccatgat
     3241 gtccatcatc cggtacgcca gccgctctga tgacactgta tacaggaatc ccttcaggta
     3301 gccctgataa ctcacaccgt tttcgcagtt ttttgccttt ttgccgtctt caaacgccgc
     3361 cgcaagactg ccaaactgcg ctgtccactg cgtctcagtt tttatcaagg caatcttatc
     3421 ccccgccagc aatgcccgga tgtccaaaat actctccaca tacgcccacg ccgcgattac
     3481 ccccgtctgg acaagccgca caaccgcagg atttcccgta aatcccgcca gcgcctccgc
     3541 cattccaagc gtctttgccc gcttgtcccc atctccgagg atatgcgtga tattggctgc
     3601 ctcccggaca aacagcaggc gcttgatgac gctttccaga ttctctttat ccgatccttt
     3661 tcctgccagc acatattcca gttcatatgc cagcgcgtga tcctctgatt ctcccagata
     3721 atctccaaaa tattgatcca tatattccag tgcgagtatt ctgtcatacc agtccgacac
     3781 ctgtatttcc atattgccgg tctgtttctg ccgcctctct atcgaatcac ccagatcggt
     3841 ctgcctcgtc gagaccgcac caaaatcccc cagcgtcatt ccaagcagtg cgttctgctt
     3901 tatcgcatga acaacctcta gcggattttc ctgctccggc ggaagttccg gtatcaccgc
     3961 ttccgtcccg tccgattctg cctgttcccg gcgttcgctt tttgcctctt cgatcgctgt
     4021 tctggcatcc tcgatactct cttccccctg tccgctctgt tccacctgct catgctcgcc
     4081 atagcgttcc tgtaatgccc gtatggtctc catcgccata ttctgcttca tatatgctgc
     4141 cacacagtgc agaaacacgc ttccctcctg atccgtcatc agacggtatg ccaccggctc
     4201 tgctcctgca agggtgacct gcaggatgct tcctcccgct ccatcaacgt ccagattccg
     4261 gtcaagccgc tctcttaaaa cagcggttac atgatccatg gaaaatatat ccccaccgta
     4321 ggcgccatcc agtcccagca gatgaaattc ttcccagagc accggctgat actctgcgca
     4381 taccgactcc agcgcaagcg tggacttcat atctgcatac gtctgtatct ggtacaccct
     4441 ccccgcctcc agaagagcca taaggaagga tgccaccagc gcaaacgcaa gtgcggaaaa
     4501 taccgtaata cttgctctct ttctcattct cagacctttc ccgccttttt cgtgatcgta
     4561 tcgaaaatat cgttcaccag acttgtaagt tgatctttaa agatcagtac cagcccgatc
     4621 aacacgacca gaatcagaat caattccaca actcccacac cgtcttcttc tctgataaac
     4681 tctctgatcc ctctcataga atccctccct ttctaaattt gaaatgccag caccgccggc
     4741 accaacagta tcaccatcac aattccgagc atcagcatca tcggaaacag caattttgtt
     4801 ccggcttcct caccgtaacg ttttgcggca tgcttccgct cctcaaacgc attctgcgct
     4861 tcctgctcca acagtaccgt aataccgcgg gttccttttt gcagattctg cgtcagtatg
     4921 ccggcaagct tccggtagga tgccacaccg caccgttctc cgaaatgctc atacgcccgc
     4981 tgttccccca caccgctctc catctgtcgg caggcgacca gcatctcctc ataggcgggc
     5041 atttccgccg ttttattctg tgtccgcttc tgtccataag ctccggcaat ttttctgaac
     5101 gctccctgaa gcgtcattcc cgcccccaga agcaaggcca gcttgctcac cagatccgga
     5161 tactcgagtt ccagaagcat tttgcgcttc ttttgctcct cctgctttct gctcatggat
     5221 accaccggta caaagcaggc gagaatcacg cccagtataa ggatcttttc cggtctctga
     5281 tcgcgccttt gctcccagtg tatggtatgt ccctccacct catccggcag aaaaagtctg
     5341 tctgctcccg cctccgactg ctgtcgcgca atcttttctg aaatcccctt ccggaagcgc
     5401 tcttcctcgt ctaacagtct gggatacacg cagatataaa actcctgtac acatctggat
     5461 tttccacatg tcagagtgac ctgcacattc accagctccc cctcctcgtc cggttctccc
     5521 acaattcttc cagtgcggct ctctatcagc cggtaattgt caaagctcca ttccgcctca
     5581 acatgccctt cctgatattc ctcacgaatg acaacagcat cccgtataca attcacagat
     5641 tcattgtctc ccgggaattc cttaatgagt tcttcctctg ccgctcgaag atatgcctgc
     5701 tcctcctgct ttgtcagctc ctgctccggc acatgcacct tgtaagcttt ggcctttttc
     5761 gccccatcca catacatacg cagtttcgag agtagcaact gtccgaaacc aacccaagt
//
LOCUS       DBMDQJ010000050         5809 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5505_length_5809_cov_1.584618, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000050 DBMDQJ010000000
VERSION     DBMDQJ010000050.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5809)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5505_length_5809_cov_1.584618"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1131)
                     /gene="tig"
                     /locus_tag="ACZO9R_01740"
     CDS             complement(<1..>1131)
                     /gene="tig"
                     /locus_tag="ACZO9R_01740"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR0911829.1"
                     /translation="LEKNMAKLTIEVAAEEVEKAIQAAYLKEKGKISMPGFRKGKVPR
                     KMIEKMYGEAVFYEDAANTLIQENYPAAVEESGIDIVSRPTIDVVQIESGKPFIFTAE
                     VAVRPEVKLGKYLGVQVTKIDTSVSDEEVEAAVEKERNNNARTVTVTDRPIANGDTAV
                     IDFEGFVDGVAFEGGKGENHPLEIGSHSFIDTFEDQLVGKNAGDEVEVNVTFPEKYQA
                     ADLAGKPAMFKVKIHEVKCKELPELNDEFAQDVSEFDTLEEYKADVKKHLEVEKENEA
                     KKTKEDEAIQKIIDKSTMEIPEAMIETQCENMVNEFAQRLAQSGLSMEQYMQFSGLTL
                     DKLKEQVRPEAETRIKSSLVLEQIAKDEKTDITDEELDAEIEK"
     assembly_gap    1132..1141
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1361..1531)
                     /locus_tag="ACZO9R_01745"
                     /pseudo
     CDS             complement(1361..1531)
                     /locus_tag="ACZO9R_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
     gene            complement(1561..2598)
                     /gene="rsgA"
                     /locus_tag="ACZO9R_01750"
     CDS             complement(1561..2598)
                     /gene="rsgA"
                     /locus_tag="ACZO9R_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR0911830.1"
                     /translation="MEHTGNKTENTKATALITAVHRERYEILVEQETSDTKLNARLKT
                     GVYYQDKGGEAFPTVGDRVRIQTNRCGDALILETLPRTSVFYRENPTPGMERQAVAAN
                     FDYVFLVMSMNHDFNQNRLDRYLTAAWESGATPAVILTKKDLCEEPEYYVNLVERQAP
                     GVAVCAVSAVTGEGMEHVQRYLGAGKTVVLLGSSGVGKSTFVNALCGETVMDTGAIRE
                     DDSKGRHTTTYRQMILLPDGSRVIDTPGMRVLGVGDAKCGMDTTFSDIEELAQHCRFR
                     DCRHEKETGCAVQEAVRDGRLPERRLLNYKKLLAEAAHAKRREEIARKKQLRSVERKK
                     NPVRKKQYKAE"
     gene            complement(3054..3605)
                     /locus_tag="ACZO9R_01755"
     CDS             complement(3054..3605)
                     /locus_tag="ACZO9R_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR0911831.1"
                     /translation="MIYEPREITCRDGRSCILRSAAVTDANDLIRYLKVTAGETPFLI
                     REPDEISLTPEQEEHFIEGIVKEPRELMLIGTVAGEHVGNCSLLSLENYERYAHRCSV
                     AIALYQKYCGLGIGREMLKTVLAVAKQCGYEQAELEVVSTNTPAIRLYESLGFEIWGT
                     QPHSVRYRDGSYADDHFMVKRLV"
     gene            complement(3602..3742)
                     /locus_tag="ACZO9R_01760"
     CDS             complement(3602..3742)
                     /locus_tag="ACZO9R_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911832.1"
                     /translation="MSEKPVFAGICGGSDTVQIDFLTITEENVEKIKCRSDTDHREET
                     QL"
     gene            complement(3870..4484)
                     /locus_tag="ACZO9R_01765"
     CDS             complement(3870..4484)
                     /locus_tag="ACZO9R_01765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0911833.1"
                     /translation="MKNIVFDMGNVLTKYKLSDYIGTYTEDEAQAALLKNQVCASVEW
                     ICMDRGTMTDEEAVRSICKRIPQSEWELVERFVREFRMKQEPNPPMEALLGELKEQGY
                     HLFLMSNTSHRFRAFSKNIPSVGYMDGIWISCECGYLKPEREAYVDFFRKMKIEPQES
                     YFVDDSPANIEAGIRLGMRGCVYHQDMPELRRNLREAGIDLKDA"
     gene            complement(4581..5102)
                     /locus_tag="ACZO9R_01770"
     CDS             complement(4581..5102)
                     /locus_tag="ACZO9R_01770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR0911834.1"
                     /translation="MQKILIVVDMQNDFIDGALGTPEAVAIVQRVEQKIREFPGKVIF
                     TRDTHEECYLETQEGKHLPVPHCIKGTKGWEICPQLEALREAEAIDKVTFGSEKLGEL
                     LQEENRKEQIEDITLVGLCTDICVISNALLLKAFLPETPIIVDASCCAGVTPKSHETA
                     LDAMRMCQINIER"
     gene            complement(5362..>5809)
                     /locus_tag="ACZO9R_01775"
     CDS             complement(5362..>5809)
                     /locus_tag="ACZO9R_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0911835.1"
                     /translation="NILYGKLDATDEEMIAAAKAVNAHEFIMKMEKGYDTELKEHGAG
                     LSIGQRQLIAFARTMISMPKILILDEATSSIDTHTEILVQKGIEALLSGRTSFVIAHR
                     LSTIQNADRIFVIDQGGILEQGSPAELMKKKGAYYDLYMAQFAGLE"
BASE COUNT         1204 a         1765 c         1121 g         1709 t
ORIGIN      
        1 cttctcaatc tcagcatcga gctcttcatc ggtaatgtct gtcttctcat cctttgcaat
       61 ctgctctaat accaggctgc tcttgatgcg tgtctcagct tccgggcgaa cctgctcttt
      121 taacttgtcg agtgtcagac cggagaactg catgtactgc tccatggaaa gaccgctctg
      181 tgccagtctc tgagcaaatt cattgaccat gttctcacac tgtgtctcga tcattgcctc
      241 cggaatctcc atggtggact tgtcaatgat cttctggatt gcctcgtcct ccttggtctt
      301 cttagcctcg ttctccttct ctacctcaag gtgcttctta acgtcagcct tgtactcctc
      361 taaggtatcg aactcggata catcctgtgc gaactcatcg ttcagttccg gaagctcctt
      421 gcactttact tcgtggatct taaccttgaa cattgcaggc tttccggcaa gatctgctgc
      481 ctgatacttc tcagggaagg ttacgtttac ttctacttcg tcgccggcat tcttgcctac
      541 gagctgatcc tcgaacgtat cgatgaagga atgggaaccg atctcaagcg gatgattctc
      601 acccttgccg ccctcaaatg caacgccatc aacaaatccc tcgaagtcga tcactgcggt
      661 atcgccgttt gcgatcggtc tgtctgtaac ggtaacagtt cttgcgttgt tatttctctc
      721 tttctctact gctgcctcta cctcttcgtc agatacggag gtatcgatct tggttacctg
      781 aacaccaaga tacttgccaa gctttacttc cggtcttaca gcaacctctg ctgtgaagat
      841 gaaaggcttg ccggactcga tctgtaccac gtcgatggtc ggtctggata cgatgtcaat
      901 accgctctcc tctactgctg caggatagtt ctcctgaatc agggtgtttg ctgcgtcctc
      961 atagaacact gcctcgccgt acatcttctc gatcatcttt ctcggaactt tacctttacg
     1021 gaaaccaggc atgctgatct tgcctttttc tttcaggtaa gcagcctgga ttgctttctc
     1081 tacttcttcg gcagccactt caatggtaag ctttgccata ttcttttcta annnnnnnnn
     1141 ntcctcctta atatatatga aatatcatgt acatcttccg tccatcgcat gacattcgtc
     1201 acgccagtcg cctgcagccc ttttttaagg gttcctgcct gtctttcgga aaaaagggcg
     1261 tactacgact atcacagtca tttaaggatt ataacataca agaccggaaa atactagctt
     1321 ttttttgatt tctttgcggt ttttccgcca aaaacacgcc ctagagctcc acaccgcggt
     1381 gttctacaga ggtagagaac acaatcagcg tctttgtccg tccaaaatac tgcaattccc
     1441 cgataaaacg gtcgagttcc atcgtatttt caaacaaaac ctccagcagc atggaatagt
     1501 cgccggtcac acagttacac tcaatcacgt tttttttcac ggtacaccct tccgcctctc
     1561 tcattcggct ttgtactgct tttttctcac ggggttcttt ttccgctcca cgctccgcag
     1621 ctgcttcttc cgcgcgatct cttcccgtcg cttcgcatgc gccgcctccg ccagaagctt
     1681 cttatagtta agcagccggc gttccggcag tcttccgtcc cgcactgcct cctgcaccgc
     1741 acaccccgtc tctttttcat gtctgcagtc tctgaatctg caatgctgcg ccaattcctc
     1801 aatatccgaa aatgtcgtat ccatcccgca ttttgcatcc cctacgccaa gtacgcgcat
     1861 tcccggtgta tctatgacac gggagccatc cggcagaagg atcatctgcc ggtaagtcgt
     1921 cgtatggcgt cctttggaat catcctcccg gatcgcgccg gtatccataa ccgtctcccc
     1981 acacagcgca ttcacaaacg tggatttgcc gacaccggat gatcccagca gcactaccgt
     2041 ttttcccgca cccagatagc gttgcacgtg ttccatccct tcgcccgtta ccgcacttac
     2101 cgcacacact gcaactcccg gcgcctgcct ctccacaaga tttacataat attccggttc
     2161 ctcgcagaga tcttttttcg tcagtatgac agccggcgtc gccccgctct cccacgcggc
     2221 tgtcagataa cggtccagcc ggttctggtt aaaatcatga ttcatcgaca tgaccagaaa
     2281 cacatagtca aagtttgccg ccaccgcctg gcgctccatg ccgggcgtcg gattttcccg
     2341 gtaaaacacg ctcgttctgg gaagcgtctc aaggatcaat gcatccccgc accggtttgt
     2401 ctgaatacgc acacggtcac ccactgtcgg aaatgcctcc ccgcccttgt cctgataata
     2461 cacaccggtc tttaacctgg cattaagctt tgtatctgac gtttcctgtt ccacaagaat
     2521 ctcataacgt tctctgtgta ccgctgtgat cagtgccgtc gctttcgtat tttctgtttt
     2581 atttcctgta tgctccatcc gttttcctct ttcctctcaa ttttccattc actgtttctt
     2641 tccattccta caatctccat tctgtttttc tccattttta cattcctcct ttattaaaaa
     2701 gaatctcgct tgcgagattc tccgcgcgcg agcggttcgc aaagcgatat tttccttacc
     2761 gcgagctgcg catcctcgcg gagcgttttt tatttcatag gaaagttcca ctttcacaca
     2821 tgaatgtatc acggtatttc ctatgaaatt aaaaagcatt cctgccacat tcctgcaccg
     2881 caatgcttct gccttcattc tatttttgtg ccggattgcc gtttccagat tgtaaaaagc
     2941 aatcgacggc ttgcaacacc catttttcaa caagcattgc cggcttgcaa catccatttt
     3001 tcaacaagca ttgccggctt ccagtctccg ggttttccaa cataatcgca gatttatacc
     3061 aggcgcttca ccataaagtg atcgtccgca tagctgccgt cccgataccg gacactgtgc
     3121 ggctgcgttc cccagatctc aaacccgagg ctctcgtaca gtcggatggc aggtgtgttt
     3181 gtgctcacca cttcaagttc tgcctgctca tatccgcact gcttcgccac cgcaagcacc
     3241 gtctttagca tttccctgcc gattccgagc ccgcagtatt tctgatagag ggcaatcgca
     3301 acactgcagc gatgcgcata gcgctcataa ttttccagag acagcaggga acagttgccg
     3361 acatgttctc cagcaacggt tccgatcaac atcagttccc gcggttcttt tacgattcct
     3421 tctataaaat gttcctcctg ctccggtgta agactgatct catccggctc acgtatcaga
     3481 aatggcgtct ccccggcagt cactttcaga taccggatca gatcatttgc atccgtcact
     3541 gctgcactgc gcagaataca gcttcgcccg tcccgacagg taatttctct tggttcataa
     3601 atcataattg ggtctcctcc ctgtgatctg tatcactccg gcactttatt ttttccacat
     3661 tttcttccgt gatcgtcaaa aagtcgatct gcacggtatc gctgccgccg caaatcccgg
     3721 caaaaacagg tttctctgac acgatgcgtc atgatagtcc cggcttttcc gctttttgtg
     3781 catccaccgg aatcggtttt tcctctttta tgcatccacc ggaacatgct tttccggtgt
     3841 cagaccttac ctggctgcgt ttgcatgtat tacgcatcct ttagatcgat tcctgcttcc
     3901 cgcagatttc ttcgcagttc cggcatatcc tgatgataaa cgcatccccg cattcccagg
     3961 cgtatccccg cttcaatgtt tgccggcgag tcgtccacaa aatagctttc ctgcggctca
     4021 atcttcattt tccggaagaa atccacatac gcttcgcgtt ccggcttcag atatccacac
     4081 tcacacgaaa tccagatgcc gtccatatac cctacagacg gaatattttt tgaaaatgcc
     4141 cggaaacggt gtgatgtatt tgacatcaaa aacaaatggt acccctgttc cttcaactct
     4201 cccagaagcg cctccatcgg cggattcggc tcctgcttca tccgaaactc cctcacgaaa
     4261 cgctccacaa gctcccactc actctgcgga attctcttgc aaatggaacg taccgcttcc
     4321 tcatcggtca ttgttcctct gtccatgcaa atccactcca ccgatgcgca aacctgattt
     4381 ttcaacaatg ctgcctgcgc ttcatcctct gtatatgtcc cgatgtaatc gctcagctta
     4441 tattttgtca gaacatttcc catatcaaat acgatatttt tcattttctc tccatattcc
     4501 gttgacctgc tgcgtcacat tgttccggcg cgtgcgcgta ctcttatctt tctatgtcga
     4561 tccggcgcat gcgcgtactt ttatctttct atgttgatct ggcacatgcg cattgcgtcg
     4621 agcgctgtct cgtggctttt cggcgttacc cctgcacagc aggatgcatc cacgataatc
     4681 ggtgtctccg gcaaaaatgc tttcaataac aatgcatttg agatcacaca aatgtccgtg
     4741 cagagtccaa ccagcgtaat gtcctcgatc tgctcttttc tattctcctc ctgcagcaac
     4801 tcgccaagct tctccgaacc gaatgtcacc ttatcaatcg cctccgcctc ccggagcgcc
     4861 tctagctgcg gacagatctc ccagcctttc gtgcccttga tgcagtgcgg caccggaaga
     4921 tgttttccct cctgtgtctc taaatagcac tcttcatgtg tatcgcgcgt gaaaatcact
     4981 tttcctggga attcgcggat tttctgttcc acccgctgaa caatggcgac cgcctccggt
     5041 gttccaagcg ctccatcaat aaaatcattc tgcatatcca ccacaatcaa tattttctgc
     5101 atcttttctc ctccttctga aacttcgcgg gtctcacggt ctctttttcc cctctcagac
     5161 ccgcagggca cctctttttc acgaatttct tctgtttttt cgctcttcgc gggtctcacg
     5221 gtctcttttt tctcttctag acccgcaggg tgaacctttc taactcggct gcagaccagg
     5281 tctactcgtc caactcagtt gcagaccaag tctacctttc cagcccaacc gcacccgcag
     5341 ccggcctcat cttccaattt tttattccag ccccgcgaac tgtgccatgt aaagatcgta
     5401 ataagcacct tttttcttca tcagctctgc cggacttccc tgctctaaga tgccgccctg
     5461 gtcgatgaca aaaatacggt ctgcattctg gatggtcgaa aggcggtgtg caatcacgaa
     5521 actggttctg ccggacagca gtgcctcaat tcccttttgc acaagaatct cggtgtgtgt
     5581 atcaatactg gacgttgcct catccagaat cagaatcttc ggcatcgaga tcatcgtccg
     5641 cgcaaatgca atcagctggc gctgtccgat cgaaagtccc gctccgtgct cctttaactc
     5701 cgtgtcatac cctttttcca tcttcataat gaactcgtgc gcattgacgg ccttcgccgc
     5761 agcgatcatc tcctcatccg tagcgtcaag ctttccatat aaaatattt
//
LOCUS       DBMDQJ010000051         5803 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5509_length_5803_cov_1.631007, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000051 DBMDQJ010000000
VERSION     DBMDQJ010000051.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5509_length_5803_cov_1.631007"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /gene="dctP"
                     /locus_tag="ACZO9R_01780"
     CDS             <1..508
                     /gene="dctP"
                     /locus_tag="ACZO9R_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HIR0911836.1"
                     /translation="LEDLKGMRIRVQESDLAVDMVEALGATAIPIAYGDVYASLERQV
                     VDGAENNWPSYEAMRHYEVAKYYTVDEHSRVPEMQICSAYTWQKLSPEDREIILECAR
                     ESAIYEREVWTQREEQSRSIAMENGTKVVELSAEEKKRFQNAVYGVYEKYCGDDMGLI
                     EEILKEGS"
     gene            588..932
                     /locus_tag="ACZO9R_01785"
     CDS             588..932
                     /locus_tag="ACZO9R_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR0911837.1"
                     /translation="MEKTVKNQVWNFNDRIDAEVCAEGVERKILAYTDELMCVENHFK
                     KGAVGALHHHPHTQITYVVSGSFEFEIDGVRRVIKPGDALLKRNDIEHGCVCLEDGIL
                     LDIFTPMRQEFV"
     gene            complement(935..2416)
                     /locus_tag="ACZO9R_01790"
     CDS             complement(935..2416)
                     /locus_tag="ACZO9R_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR0911838.1"
                     /translation="MRKLSSVILADLVASKRKEKSMTQQSLAEATGINRALISRIEKQ
                     DFIPSIPQLEQLGEVLGFEPDSVFADTAHDRLPSPSPLRIAVAGTGYVGLSIATLLAQ
                     HNHVTAVDILPEKIDLINRRKSPIQDDYIEKYLAEKELDLTATLDGAAAYRDADYVII
                     AAPTNYDSTRNYFDTSAVEAVIELVLSVNPDAVMVIKSTIPVGYTESVRQKYHTANII
                     FSPEFLRESKALYDNLYPSRIIVSTDSDDARLSAAARNFAALLQEGALKENIPTLFMG
                     FTEAEAVKLFANTYLALRVSYFNELDTYAETKNLSTRDIIDGVCLDPRIGTHYNNPSF
                     GYGGYCLPKDTKQLLANYNDVPQNMMSAIVESNRTRKDFIADRVLQMAGYYNYASANT
                     YNSGMEHPVTIGVYRLTMKSNSDNFRQSSIQGVMKRIKAKGASVIIYEPTLEDGTTFF
                     GSKVVNRLDEFKKISDSIIANRYDSCLDDVKDKVYTRDLFRRD"
     gene            2692..3318
                     /locus_tag="ACZO9R_01795"
     CDS             2692..3318
                     /locus_tag="ACZO9R_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911839.1"
                     /translation="MGGLIWIIVIVVVISEIKKFQRKNGQRVKPNVPRQQQYMRPDQT
                     KVGQQNVRPNQMPRQQQNVRPNQMPRQQQGMRPNQIPWQQNVRPNQMAGRQNVRPNRM
                     PGQRTQSAGGAAMAGCRRPQPNQAENDILKRATENVSENDGDELEQQMMAGAHELVNA
                     IDIDSASELMHEVEDLMIMGYQANLPFERDFIAEGVAMLNGYELDMGA"
     gene            3364..3981
                     /locus_tag="ACZO9R_01800"
     CDS             3364..3981
                     /locus_tag="ACZO9R_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HIR0911840.1"
                     /translation="MERVRIPQSGEIYRHFKNRLYQVIAVATHSETGEKLVIYQALYG
                     DYQVYARPLAMFVSEVDREKYPDAAQTYRFERVDRSELAGAGSGRMERAEARKQAQAT
                     ASFADEVQETEAEELTDQIRVSQMEQSLSDEVNPKLMQFFDADTLEEKYNILVSMRDE
                     VDDHLINSMAVVLDVVIPEGPVADRYEQLKACIRTKQRYETQRMR"
     gene            4006..5187
                     /locus_tag="ACZO9R_01805"
     CDS             4006..5187
                     /locus_tag="ACZO9R_01805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0911841.1"
                     /translation="MEEQKEKLSAIAQAKPYKIIISKPAGGEIPYRRIVIERKESYYQ
                     AAAYTEKQVFHENIPPEKLAEYLGKTAVGQYLQVNAWDDAAEHIYLCSKKGKVTYKTK
                     KNVQTAAKASGSHNRKKQYILEEGTPIEPLVDMGVFTPEGKVVRTMYDKFRQINRFLE
                     MVGDAVGDDTSETLHIIDFGCGKSYLTFVLYYYFTEIQKRNVQIAGLDLKADVIKNCN
                     AAAKKYGYENLHFEVGDINGYRTDAPVDMVVTLHACDTATDYALYNAIMWNARMILSV
                     PCCQHELNGQIQSEDLGLMTRYGIIKERFAALATDAIRANLLECCGYKTQVLEFVDFA
                     HTPKNLLIRAVKKGGSDAARAIVPAAVRKRYLGEVERMMEEFHLDPTLYRLLKEAGRL
                     G"
     gene            complement(5197..5652)
                     /locus_tag="ACZO9R_01810"
     CDS             complement(5197..5652)
                     /locus_tag="ACZO9R_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR0911842.1"
                     /translation="MEIERKYLVKTLPDHLEQYPCKQIEQGYLSTNPVVRIRRSNDRY
                     ILTYKGKGLMVREEYNLPLNEESFTHLKEKIDGLLIQKRRYLIPFAEKYTIELDVFEG
                     ALAPLVLAEVEFETEEEANTFVPPEWFGEDVTFSTKYHNSTLSQRTFSS"
BASE COUNT         1500 a         1276 c         1770 g         1257 t
ORIGIN      
        1 tctggaggat ctgaaaggca tgcggatccg tgtgcaggag tcggatcttg ccgttgatat
       61 ggtggaggcg ctcggcgcta cggcgattcc gattgcctat ggggatgttt atgccagcct
      121 ggagcggcag gtggtggatg gagccgagaa taactggccg tcctatgagg cgatgcggca
      181 ctatgaggtc gcgaaatact atacggtgga cgagcacagc agagtgccgg aaatgcagat
      241 ctgctcggcg tatacctggc agaaactgtc gccggaagac cgggagatta tcctggaatg
      301 tgccagagaa tcggcgatct acgagcgtga ggtgtggaca cagcgcgagg agcagtcgcg
      361 cagcattgcc atggaaaacg gcacgaaagt ggtggagctt tccgctgagg aaaaaaaacg
      421 gttccagaat gccgtgtacg gggtatatga aaaatactgc ggggatgaca tgggactcat
      481 cgaagaaatc ttaaaagagg gatcgtaatg cggcgggcag aatgtgcaga cggtcgggaa
      541 aaatgccgca tacagataaa aacaaaaaga aaaagggaga tggagcaatg gagaaaacag
      601 taaaaaatca ggtgtggaac tttaatgaca ggatcgatgc cgaggtgtgc gccgagggcg
      661 tggaacgcaa gattttagcg tacacggatg aactgatgtg cgtggaaaac cacttcaaaa
      721 aaggagcggt cggcgcactg catcatcatc cgcatacgca gatcacatat gtggtgtcgg
      781 gcagctttga attcgagatt gacggcgtca gaagagtcat caaaccgggc gacgcactgt
      841 taaagcgcaa cgacatcgag cacggctgcg tctgtctgga agacggcatc ctcttagaca
      901 tctttacccc gatgcggcag gagtttgtat aaaactaatc ccgccggaac aaatctctcg
      961 tgtagacttt gtcttttaca tcatcgagac aggaatcata gcggttggcg atgatgctgt
     1021 cgctgatctt cttaaactcg tcaagccggt tgaccacctt gcttccgaag aaggtggttc
     1081 cgtcttcgag tgtcggttcg tagataatca cggaagcccc ctttgctttg atgcgtttca
     1141 tgacgccctg gatactgctc tgccggaagt tgtcagagtt ggatttcatc gtcaggcggt
     1201 agacgccgat ggtgaccgga tgctccatgc cggaattgta ggtgttggcg gaggcgtagt
     1261 tgtagtatcc tgccatctgc agaacgcggt cggcgataaa atctttgcgg gtgcggttgc
     1321 tctccacgat tgcgctcatc atgttctgcg gaacgtcatt gtaattagcc agaagctgtt
     1381 tggtgtcctt tggcagacag taaccgccgt atccgaagga cgggttgttg taatgcgtgc
     1441 cgattctcgg gtcgagacat acgccgtcga tgatgtcgcg tgtgcttaaa tttttcgtct
     1501 ccgcataggt gtcaagctca ttgaaatagg acacgcgcag cgccagatag gtgtttgcaa
     1561 aaagtttgac ggcttccgcc tcggtaaatc ccatgaaaag cgtcgggata ttttccttga
     1621 gggcgccctc ctgcagcagt gcggcaaaat ttctcgcggc ggcagaaagc cgcgcatcgt
     1681 cggagtcggt cgagacgatg atccggctcg ggtagagatt gtcatataac gccttcgact
     1741 cgcggagaaa ttccgggctg aagatgatgt ttgcggtgtg gtatttttgg cgtaccgatt
     1801 ccgtgtagcc gaccgggatc gtcgatttga tgaccatgac cgcgtccgga ttgacggaga
     1861 gaaccagttc aatcacggcc tccacagcgg aggtgtcaaa ataattcctg gtgctgtcgt
     1921 agttggtcgg tgcggcgatg atgacgtaat cggcgtccct gtatgctgcg gcgccgtcta
     1981 aggtggcggt gagatcaagc tctttttcgg cgagatattt ttcaatataa tcgtcctgaa
     2041 tcggggattt gcgccggttg atgagatcga ttttttccgg aagaatgtcg accgccgtca
     2101 catggttgtg ctgcgcgagc agggtggcga tggagagtcc gacgtagccg gtgccggcaa
     2161 ccgcaatgcg taacggtgac ggggagggaa gcctgtcgtg cgcggtatca gcgaaaacgc
     2221 tgtccggctc aaagccgagc acttctccca gctgctctaa ctgcgggatg gacgggataa
     2281 aatcctgttt ttcaattctt gagatcaggg cgcggttgat cccggtcgcc tcggcgaggg
     2341 actgctgcgt catggacttc tcttttctct tgctggcgac gaggtccgcc agaatcacag
     2401 atgataattt cctcatgaaa tctcctcctt gcatgttatt taaaataact atatctgcaa
     2461 aacaatctga taaaagcata tcacaagacg aaaataaaag caatattgga taataatgat
     2521 atttaaaata acaacgcgaa gacaaaaaag agagtggatg tagaagaact gtaccgtagg
     2581 ggaaagttct ggttgagaaa caaaaaagaa aacactataa taagaataag tggggcgaag
     2641 ggctctgcat gaatgacagg aacaaaagag gattggaata agggggaagc tatgggcgga
     2701 ctgatatgga tcatcgtgat tgtagtggtg ataagtgaga taaagaaatt ccagagaaag
     2761 aacggacagc gtgtgaaacc gaatgtgccg aggcagcagc agtacatgcg tccggatcag
     2821 acaaaggtag gacagcagaa tgtgcgcccg aatcagatgc cgaggcagca gcagaacgtg
     2881 cgcccgaacc agatgccgcg ccagcagcag ggcatgcgcc cgaatcagat accttggcag
     2941 cagaatgtgc gcccgaacca gatggcagga cggcagaatg tgcgcccgaa ccggatgccg
     3001 ggacagcgga cgcagagtgc aggcggggct gccatggcag gctgccgcag accgcagcct
     3061 aatcaggcgg agaatgacat attaaaacgt gccacagaga atgtgagcga gaacgatggg
     3121 gatgaactgg agcagcagat gatggcggga gcacacgaac tcgtcaacgc gatcgatatt
     3181 gacagtgcca gtgaactgat gcatgaggtg gaggatctga tgattatggg ttatcaggca
     3241 aatctgccgt ttgagcgcga ttttatcgcg gagggagtgg caatgctgaa cggttatgag
     3301 ctggatatgg gtgcataaag atacggcagg acagcaaaga gggacagaag gaaggttaca
     3361 ggaatggaaa gagtgagaat cccgcagagc ggggagatct atcgtcactt taaaaacaga
     3421 ctttatcagg tcatcgcagt ggcaacacat tcggagaccg gagaaaaact ggtcatttat
     3481 caggctctct acggagatta tcaggtatat gcgagaccgc tcgccatgtt tgtgagcgag
     3541 gtcgatcgtg aaaaatatcc ggatgcggcg cagacctacc gttttgaacg cgtggaccgg
     3601 agcgaacttg cgggcgctgg cagcggacgc atggagcggg ctgaggcgcg gaagcaggca
     3661 caggcgacgg catcgtttgc ggacgaggtg caggagacgg aggcagaaga gctgacggat
     3721 cagatccggg taagccagat ggagcagtcg ctttccgatg aggtgaatcc gaagctgatg
     3781 cagttttttg acgctgatac gctggaagaa aaatataata ttctggtgtc gatgcgggat
     3841 gaggtggacg accatctcat caacagcatg gcggttgtgc tggatgttgt gattccggag
     3901 ggaccggtgg cggaccgcta tgagcagttg aaagcctgca tccgcacaaa gcagcgctac
     3961 gagacgcagc ggatgcgctg accagatacg agagcaggag agtacatgga agaacagaag
     4021 gaaaaattat ctgcgattgc gcaggccaaa ccatataaaa tcattatcag caaaccggcg
     4081 ggtggggaga tcccttaccg ccggattgtc attgagcgca aggaatccta ttaccaggcg
     4141 gcagcctata cggaaaaaca ggtatttcac gagaacatac cgcccgagaa gctggcggaa
     4201 tatctgggga aaacggccgt ggggcagtat ctgcaggtca atgcctggga tgatgctgca
     4261 gagcatatat atctgtgttc caaaaaggga aaagtaacct acaagacgaa aaagaatgtg
     4321 cagacggcgg caaaggcaag cgggagccac aaccgcaaaa agcagtatat tctggaggag
     4381 ggaacgccga tagaaccgct tgtggatatg ggagtgttta cacccgaagg aaaagtcgtg
     4441 cgcacgatgt atgacaagtt ccggcagatc aaccgttttt tggagatggt cggtgatgcg
     4501 gtcggggacg atacatcaga aacacttcac atcattgatt ttggctgtgg aaaatcttac
     4561 ctgacattcg tgctgtatta ctattttacg gagattcaga aaagaaacgt gcagattgca
     4621 gggcttgacc tgaaagcgga cgtgattaag aactgcaatg ccgccgcgaa aaagtacggc
     4681 tatgagaatc tgcattttga ggtgggagac attaacggat accgcacgga tgcgccggtg
     4741 gatatggtgg tgacgcttca cgcctgcgat actgcgacgg attatgcact gtacaacgcc
     4801 atcatgtgga acgcgcgcat gattctttcc gtgccgtgct gccagcatga attaaacgga
     4861 cagatccagt cggaggatct gggactgatg acgcgctacg gaattattaa ggagcgcttt
     4921 gcggcacttg ccacggacgc aatccgcgcg aatctgcttg agtgctgcgg ctataagacg
     4981 caggtgctgg aatttgtaga ctttgcccat acgccgaaga accttctgat ccgcgcagtg
     5041 aaaaaaggcg gatccgatgc ggcgagagcg atcgtgcctg ccgccgtccg caagcggtac
     5101 ctcggcgagg tggagcgcat gatggaggag ttccatctgg atccgacttt gtaccgcctg
     5161 ttaaaggagg cggggcgcct gggctgatgc cccggttcag gaagaaaagg tgcgctggct
     5221 taaggtgctg ttgtgatatt ttgtggagaa cgtgacgtcc tcgccgaacc actcgggcgg
     5281 gacaaacgta ttcgcttcct cctcagtctc aaactcgact tccgcgagca cgagcggggc
     5341 aagcgctccc tcaaatacat ccagctcaat ggtatatttt tccgcgaatg gaatcagata
     5401 gcggcgcttc tgaatcagaa gaccgtcaat tttttctttt aaatgggtga aagactcttc
     5461 gttcagggga agattatatt cctcgcggac catgagtcct tttcccttgt aagtcagaat
     5521 atagcggtca ttgctgcgcc ggatgcgcac gaccgggttc gtgcttagat agccctgctc
     5581 aatctgcttg caggggtact gttccaagtg gtctgggagg gtcttgacga gatacttgcg
     5641 ttcgatttcc atgtgaattc ctttccggct gccgcaggga gtgttccggc tggcggcaga
     5701 catgctgatg cattgattgt gtattgattc tatcatagca cgtttttgca aagtgtggta
     5761 ctcttttgat agggcgaaag cccgacagcg aggaatgcgc agc
//
LOCUS       DBMDQJ010000052         5803 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5510_length_5803_cov_1.461066, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000052 DBMDQJ010000000
VERSION     DBMDQJ010000052.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5510_length_5803_cov_1.461066"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(16..1686)
                     /locus_tag="ACZO9R_01815"
     CDS             complement(16..1686)
                     /locus_tag="ACZO9R_01815"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR0911843.1"
                     /translation="MKSDIEIAQEAQMVHIREVAAKLDIPEDDLELYGKYKAKLSDEL
                     IRRVSDRKDGKLILVTAINPTPAGEGKTTTSVGLGEAFGRLGKRAVIALREPSLGPCF
                     GIKGGAAGGGYAQVVPMEDLNLHFTGDFHAITSANNLLAALLDNHIQQGNELGIDPRQ
                     IVWKRCMDMNDRVLRNIVVGLGSKMDGMVREDHFVITVASEIMAILCLADDMHDLKRR
                     LGRIIVAYTFDGKPVTADDLKATGAMAALLKDALKPNLIQTLEHTPAIVHGGPFANIA
                     HGCNSVRATKTALKLADYVITEAGFGADLGAEKFFDIKCRKAGLAPDAVVLVATVRAL
                     KYNGGVAKADLGTENLDALKKGIVNLEKHIENLQKFGVPVVVTLNSFVTDTKAETDYV
                     EQFCRERGCEFALSEVWEKGGEGGIELANKVLLTLEQKESHFKPLYPDDMSLEDKIAT
                     VAREIYGADGVTYSAAAKKELKRITDMGMSSFPVCMAKTQYSLSDDQTKLGRPNGFTI
                     NVREVYVSAGAGFVVAVTGSIMTMPGLSKNPAAYGIDVNDDGVITGLF"
     gene            complement(1742..>1864)
                     /locus_tag="ACZO9R_01820"
     CDS             complement(1742..>1864)
                     /locus_tag="ACZO9R_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR0911844.1"
                     /translation="AARIMDQLEEAGIVGPEEGTKPRRVLMSPEQFEQYEEEYL"
     assembly_gap    1865..1874
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1875..4534)
                     /locus_tag="ACZO9R_01825"
     CDS             complement(<1875..4534)
                     /locus_tag="ACZO9R_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR0911845.1"
                     /translation="MATGNKKSTSGKRKAQTSRKGSTGRKKKTAAQQQEDFTREVILW
                     IIVAVSILLFISNFGIGGTIGNAVSRFFFGVMGLIAYVFPVLLLVGTFFAVSNRGNRV
                     ATVKLVAMILFDLFLCMLIELLTKGSAVDGAAAAYSYSFEHRTGGGFIGGLLAWIFCP
                     NFGLAGSYVIDAIMLIISLVLITERSALRGMQKGGKKVYESARSGNERHKERVRIQRE
                     EREQRREEQALRRMDRKVEGVAIDTRVLPRQNALEHSDEISELNAEDYLEMPEVLEEK
                     IVPLTADGGYPSAENLTPSAFSDEALAAVTPEASQNISAWTPETETARGASWNTAPAE
                     ASWDVATEPEDPWSTTASQEQKDPWSSAAEPEDPWSTASAQEQKESWGSAAGPENAWE
                     AAAPKVQLKEPQEASAAVSPAETKAVSGIGENAGHTTSAPAASGESVSAEQMPPERPY
                     VFPPADLLTKAANKAGDSRQHLQETAMKLQQTLKNFGVNVTITNISCGPAVTRYELQP
                     EMGVKVSKIVNLADDIKLNLAAADIRIEAPIPGKAAIGIEVPNKENVMVSFRELVESE
                     EFKKHPSKISFGVGKDIGGKVTVADIAKMPHLLIAGATGSGKSVCINTIIMSILYKAN
                     PKEVKLIMIDPKVVELSVYNGIPHLMIPVVTDPKKAAGALHWAVDEMTDRYQKFANAS
                     VRDLKGYNAKIESLPTIEGDPKPEKLPQIVIIVDELADLMMVSPGEVEESICRLAQLA
                     RACGIHLIIATQRPSVNVITGLIKANMPSRIAFAVTSGVDSRTILDVNGAEKLLGKGD
                     MLFSPQGIPKPVRVQGAFVSDEEVSAVVGFIKEQNGQVTYSAEMEEKLSNMESANTTV
                     AIDSSADAGDGRDVYFADAARLLMEKEKGS"
     gene            complement(4557..5435)
                     /locus_tag="ACZO9R_01830"
     CDS             complement(4557..5435)
                     /locus_tag="ACZO9R_01830"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR0911846.1"
                     /translation="MSLLQAILMGIIQGLTEFLPVSSSGHLALFKILFDVNTDTGILY
                     DVLLHVGTLIAISLVYYRDILKLVVDGCCIIRDAGYNVIVFFRNKTGKEQMAYRRVIN
                     SSYRKFAMLIIVSTIPTGIIGYFCKDVVEMASEILIVPGICLIATAVLLLIADHCKDG
                     DKLPKNVTYTDAFSVGIAQGIATLPGLSRSGTTITACLLAGYQRNFAVKYSFLMSIPA
                     ILGALVLELKDATDLAVSGTEVVYYVIGMAVAAVVGYVCIKTMLVIVRKKKFTVFAIY
                     CLLVGALSIGGYFYLA"
     gene            complement(5531..>5803)
                     /locus_tag="ACZO9R_01835"
     CDS             complement(5531..>5803)
                     /locus_tag="ACZO9R_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease"
                     /protein_id="HIR0911847.1"
                     /translation="SGTVIGAKPTYDYFKQMQNRILTFIASHSKAGEERLEELMMNTG
                     MLTKDLGTILVGEEAVREGLIDAVGGIDAAFLKLHAMIDAAKGKNY"
BASE COUNT         1286 a         1736 c         1304 g         1467 t
ORIGIN      
        1 cctccattct ggctcttaga ataatccggt aatgactccg tcatcattga catcaatacc
       61 gtacgccgcc gggttcttgg aaagtcccgg catcgtcatg atcgaaccgg taactgcaac
      121 gacaaagccg gctcctgccg atacgtaaac ctcgcgcaca ttgatggtaa aaccgttcgg
      181 tcttccaagc ttcgtctggt catccgaaag agaatactgt gtctttgcca tgcagaccgg
      241 gaaggatgac attcccatat cggtaattct cttcagttcc tttttggcag ctgcggaata
      301 ggtcactccg tcggctccgt aaatctctct cgcaacagtt gcgatcttat cctcaaggct
      361 catatcatcc ggatataatg gcttgaaatg gctttccttc tgctccagtg taagcagcac
      421 cttgttggca agctcaatgc cgccttcacc gcccttttcc cagacctcgg aaagtgcaaa
      481 ctcacatccg cgttctctgc agaactgctc cacgtaatcc gtctccgctt ttgtgtctgt
      541 gacaaaggag ttcaaggtaa ccacaaccgg cacaccgaac ttctgcaggt tctcaatatg
      601 cttctccagg ttgacaattc ctttcttcaa cgcgtccaga ttctctgtgc caagatccgc
      661 tttcgccacg ccgccgttgt atttgagtgc acgcacagtt gcaaccagca caacggcatc
      721 cggtgccagt cctgctttcc ggcacttgat atcgaagaat ttttcagcgc cgagatctgc
      781 gccaaagccg gcctccgtaa tcacataatc cgcgagcttt aaggctgtct ttgtagcacg
      841 gacactgttg catccgtgcg caatattggc gaacggacct ccgtgaacaa tggcaggcgt
      901 gtgctctaac gtctggatca ggttcggctt tagcgcgtcc tttaacagtg ccgccatcgc
      961 gccggtcgct ttcagatcgt ccgcggtcac cggctttccg tcaaacgtat atgcgacgat
     1021 aatccgtccg agccggcgct ttaagtcatg catatcgtca gccagacaaa gaatagccat
     1081 aatctccgac gcaaccgtga tgacaaagtg atcttctctt accattccgt ccatcttgct
     1141 tcccagaccg acgacaatgt tacggaggac acggtcgttc atatccatgc agcgtttcca
     1201 gacgatctgc cgcggatcaa ttccaagctc attgccctgc tggatatggt tgtccagaag
     1261 agccgccagg agattgttgg cggaggtaat tgcatggaaa tcaccggtaa agtgcagatt
     1321 cagatcctcc atcggcacaa cctgcgcgta tccgccgccg gctgcacctc ccttgattcc
     1381 aaaacacggt ccaagggacg gctctcttaa cgcgatgacc gcccttttcc caagtctgcc
     1441 gaatgcctcg ccgaggccga cgctcgtggt ggtctttccc tctcccgccg gcgttggatt
     1501 gattgcggtc acaagaatca gtttcccgtc ttttctgtcg gaaacccgac ggatcagctc
     1561 gtccgaaagc tttgccttgt actttccgta caattccaga tcatcctccg ggatgtcaag
     1621 ctttgcagcc acctcgcgaa tatgtaccat ctgggcttcc tgtgcaattt caatatcact
     1681 tttcataatg tctcctccat tccgccgcca gagcggcttt gtatgtttta tatgaatcct
     1741 gttacagata ttcctcttcg tactgctcaa actgctcggg cgacattaaa acccgccgcg
     1801 gctttgtgcc ttcctcagga cctacgatac ccgcttcctc cagttgatcc ataatgcggg
     1861 ctgcnnnnnn nnnnatggaa cctttttcct tctccatgag aaggcgcgcc gcatctgcaa
     1921 aataaacgtc cctgccgtcc cctgcatccg cgctggaatc gatcgctacg gtggtgttgg
     1981 cggattccat gttgctcagc ttctcctcca tctccgcact gtaggtcacc tgaccattct
     2041 gttccttgat aaaccctaca acggcagata cttcctcatc cgatacaaat gcgccctgca
     2101 cacgcaccgg ctttggaatt ccctgcgggc tgaacagcat gtctcccttg ccgaggagct
     2161 tctcggcgcc gttcacatcc agaatggttc tcgaatccac gcccgaagtc acggcaaagg
     2221 cgatacggct cggcatattc gccttgatca gaccggtaat gacattgacc gacggacgct
     2281 gtgtcgcaat gatcaaatga atgccgcacg ctcttgcgag ctgtgccaga cggcagatgg
     2341 attcctcgac ttcgcccggc gataccatca taagatccgc cagctcgtcc acgataatca
     2401 caatctgcgg caacttttcc ggcttcggat cgccctcgat cgtcggcagg gattcgatct
     2461 tcgcattgta gccctttaag tctctcaccg aggcattggc gaacttctga taacggtctg
     2521 tcatctcatc caccgcccag tgcagtgcac ccgccgcttt tttcggatcg gtgaccaccg
     2581 gtatcatcag gtgcgggatg ccgttataga cactcagttc cacaaccttc ggatcaatca
     2641 taatcagctt gacctctttg gggttcgcct tgtagaggat gctcatgata atcgtgttga
     2701 tacatacgga cttaccggaa cccgtcgcac cggcaatcag aagatgcggc atcttggcaa
     2761 tgtccgccac cgtcaccttt ccgccgatgt cctttccgac gccgaaagaa atctttgacg
     2821 gatgcttttt aaattcttcc gactctacca gttctctaaa agaaaccatg acgttttctt
     2881 tgttcggaac ctcaataccg atcgccgcct ttccggggat cggtgcctcg atacgaatat
     2941 ccgccgctgc cagattcagc ttgatgtcgt cggccagatt gacaatcttg ctgaccttta
     3001 ctcccatctc cggctgcagt tcatagcgcg tcacggcagg accgcagctg atgttggtaa
     3061 tcgtgacatt cacgccgaaa ttctttaagg tctgctgcag cttcatcgcc gtctcctgca
     3121 gatgctgtct ggaatccccg gctttgtttg ccgcctttgt cagaagatct gccggaggga
     3181 acacatacgg acgctccgga ggcatctgct ccgccgacac actttcaccg gatgccgcag
     3241 gtgcagatgt ggtatgtccg gcattttccc cgatgccgga cacagccttt gtctctgcgg
     3301 gcgatacggc tgccgatgct tcctgcggct ctttcagctg tactttcggc gcagccgcct
     3361 cccatgcgtt ttctggtccg gccgcactgc cccatgattc tttctgttcc tgcgcagatg
     3421 cagtgctcca cggatcttcc ggttcggctg cgctgctcca cggatctttc tgttcctgcg
     3481 aggctgtagt gctccatgga tcttccggct ctgttgccac atcccacgat gcttccgccg
     3541 gcgcagtatt ccaggatgct ccccgtgcag tctccgtttc cggagtccat gcagatatgt
     3601 tctgtgaagc ctccggtgtc acagccgcaa gggcttcgtc agagaatgca gacggcgtca
     3661 gattctctgc agacggatat ccgccgtctg ccgtgagtgg cacaatcttt tcttccagta
     3721 cctccggcat ctccagataa tcctccgcat tcagctcact gatctcatcg gaatgttcga
     3781 gggcattctg ccgcggcagc acccgggtgt caatggcaac cccttccacc ttgcgatcca
     3841 tccgccttaa tgcctgttcc tctctgcgct gctcccgctc ttcccgctgg attctcacgc
     3901 gttccttatg gcgttcattt ccgctacgcg cggattcgta gacctttttc ccgccttttt
     3961 gcattccgcg aagcgcagag cgctctgtaa ttaacaccag cgatatgatt aacatgatcg
     4021 catcgatcac ataagaacca gccagaccga aattcgggca gaaaatccat gccagaagtc
     4081 cgccgatgaa gccgcctccc gtccggtgct caaaactgta gctgtatgcc gcagcggcac
     4141 cgtcaaccgc agaccctttg gtcagcagtt cgatcagcat acataagaac agatcaaaca
     4201 gaatcatcgc cacaagcttt acggttgcca cccggttgcc cctgttcgac acggcaaaaa
     4261 aagtaccgac aagaagaagc accgggaaca catacgcgat cagtcccatg acgccaaaaa
     4321 agaacctgct cactgcattg ccgatggttc ccccgattcc gaaattactg ataaataata
     4381 aaatagatac cgctacaatg atccagagaa tgacctccct ggtaaaatcc tcctgctgct
     4441 gtgcagccgt ctttttcttc cgtcctgtac ttcccttccg gcttgtctgt gcctttcgtt
     4501 tcccagatgt actcttttta ttccctgttg ccaaaactta ctaacctcca aaaccgttat
     4561 gccagataaa aatacccgcc gatggacaat gcgccaacga gcagacagta gatggcaaaa
     4621 acggtgaact tctttttgcg gacgatgaca agcatcgtct taatgcagac atatcccaca
     4681 accgccgcga ccgccatacc gatcacatag tagacaacct ccgttcccga caccgccagg
     4741 tctgtggcat cttttaattc gagaacaagc gcgccaagaa tcgccggtat cgacatcaaa
     4801 aacgagtatt tcacggcaaa attgcgctga taacccgcaa gcaagcaggc ggtaatcgtc
     4861 gtaccggagc gtgaaagtcc cggaagcgtt gcgattccct gtgcgattcc gacagaaaac
     4921 gcatccgtgt aggtgacgtt cttcggaagt ttgtcgccgt ccttgcagtg atccgcgatc
     4981 aaaagcagca ctgccgttgc gataaggcag atgcctggaa caatcagaat ctcggaagcc
     5041 atctccacga catccttgca gaaatagccg ataattccag tcggtattgt cgatacgata
     5101 atcagcatgg caaatttgcg gtagctgctg ttgatcacgc gccgatacgc catctgctct
     5161 ttgccggtct tattccggaa gaagacaatc acattgtatc cggcgtcgcg gatgatgcag
     5221 catccatcta ccaccagttt taagatgtcc cggtagtata ccagggagat tgcaatcaat
     5281 gtaccgacgt gaagcagtac atcatataaa attcccgtgt cggtattgac atcaaacagt
     5341 attttaaaaa gtgccagatg cccggaactg ctcaccggca aaaactcggt cagtccctgt
     5401 ataatgccca tcaagatcgc ctgtaataat gacatggcag cctccttatc cgattcgtaa
     5461 aaatctgtca aacttataca tacatgacca attttaacat atttgagggc ggttgtcatt
     5521 aaaaggtttc ttaataattt tttccctttg cggcatcaat catcgcatgc agttttaaaa
     5581 acgcggcatc gattccgccc acggcatcga tcagcccttc ccgaaccgct tcctccccga
     5641 ctaaaatggt tccaaggtct tttgtcagca ttcccgtgtt catcatcagt tcctccagac
     5701 gctcttcccc cgcctttgaa tgcgacgcga taaaggttaa aatccggttc tgcatctgtt
     5761 taaaataatc gtaggtcggc ttggctccta tgacagttcc gct
//
LOCUS       DBMDQJ010000053         5795 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5525_length_5795_cov_1.387601, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000053 DBMDQJ010000000
VERSION     DBMDQJ010000053.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5795)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5795)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5795
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5525_length_5795_cov_1.387601"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="ACZO9R_01840"
     CDS             <1..1038
                     /locus_tag="ACZO9R_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911848.1"
                     /translation="DFSLEIKEGEFLILVGPSGCGKSTLLRMIAGLEDISSGELWVDG
                     EIENYKEPRERNLAMVFQNYALYPNMSVYDNIAFSLSVRKVDKKEIKKKVYDTAKMLG
                     IEHLLDRRPGELSGGQKQRVAIGNAIIRNPRALLMDEPLSNLDAKLRTQMRVELAALQ
                     HAVGTTTIYVTHDQTEAMTLGTRIVVMRDGKMQQVDTPANLYAKPVNRFVAGFIGTPS
                     MNFFEAQVVSWEEAPALLVGEYRMMPCREDAARISRHGSDSVILGIRPENILWEAPEC
                     VSAGADYVETMILEVELLGAEKLLFFLLYGKKYVAKIPAEYDARAGEVRRLYFDVTAI
                     HLFDCDSEMRI"
     gene            1050..1919
                     /locus_tag="ACZO9R_01845"
     CDS             1050..1919
                     /locus_tag="ACZO9R_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0911849.1"
                     /translation="MNAFVKLRQKISPYLFLVPCLIVFGLFLFYPFAKTIYLSLYKTD
                     KLGQAKLFVGFGNYIDLFTSESFYNSLLVTLIFVVIVVMVSMLLGLVTALLVNKNFPG
                     IRVFSTAYALPMAIASSAAALIFEIMLDPTIGILDKFLRSDINWLHDERYALVCVALL
                     TAWLNSGINYLYFSAGLANIDESIYERASVDGANEWQKFTRLTLPGLSPILFYTVIVN
                     IILAFQSFGQVKILTEGGPGESTNLIVYSIYRDAFFNFRFGGAAAQSVVLFLIVMLLT
                     LLMFRLEKKGVKY"
     gene            1991..2830
                     /locus_tag="ACZO9R_01850"
     CDS             1991..2830
                     /locus_tag="ACZO9R_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0911850.1"
                     /translation="MKKRELGRKRRRLWSRFGVNLVVSVLVILPLLYALSIAFMPSGE
                     LFSMEMNLIPKQPTLDNFAQAFTKIPLFRFILNSFLVAGCITLGQIISCSLAAFALSF
                     LDFKGKNVIFMLIMATMMVPGEATIISNYLTVSGWGWLNSYKVLIIPYITSAMGIFLF
                     RQFYLTFPKSLYEAAKIDGCPNLVFIVRILISLTKSAIGAMAVYTFINAYNMYMWPLL
                     VTGTEEMRTVQIGISMLNSVDSQSITMVIAGVVMVILPSLFVFILGQKQLIRGMFSGA
                     VKG"
     gene            2876..4303
                     /locus_tag="ACZO9R_01855"
     CDS             2876..4303
                     /locus_tag="ACZO9R_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0911851.1"
                     /translation="MKKRIIASLLTASMLAAALAGCGGSTAQTADTSADAAGEAESAQ
                     GDPATVTEKETNVSATADADLSGTTITFWHSMSGVNGEALTALVDRFNEENTYGITVD
                     AEFEGEYDDAINKLKSSMVGGSGPDLIQVYEIGTRFMIDSGWVIPMQELMDANGYDNS
                     NIVPNIAAYYTIDDQLYSMPFNTSTPILYYNKDMFDAAGITEVPTSLEGIDAIADDLV
                     NKGGAGEALSMGIYGWFFEQFICKQGLAYANNDNGRSAAATAVDFDGNGAALSIVSAW
                     KDLYDKGYAPNVGVGGDAGLTDFSAGKAAITLGSTASLKQILNDVNGSFEVGTAYFPG
                     IKDTDQGGVSIGGASLWAIQNQDDVKAQATWKFVEYLVSAESQAYWATQTGYFPVTND
                     AYNEDVFKQNIEQYPQFQTAIDQLNDTKGEYAGALLSVFPEARQTVQTEIENTLNDKE
                     TPEEAVQKMADTINASIEDYNLLNE"
     gene            4627..5127
                     /locus_tag="ACZO9R_01860"
     CDS             4627..5127
                     /locus_tag="ACZO9R_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR0911852.1"
                     /translation="MALLEQWQKVAYNEKADRGELQRFWQRYFLLEKGVYEKLLTNPD
                     EKVEGTVKELADKYGLSIMDMTGFLDGINESLVEPNPIDTMEEDTKVSLVFDKEKLYK
                     NMVDAKADWLYELPMWNDIFDPETKKRLYLEQKKSGTVIVGKKVGRNDPCPCGSGKKY
                     KFCCGK"
     gene            5305..5622
                     /locus_tag="ACZO9R_01865"
     CDS             5305..5622
                     /locus_tag="ACZO9R_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HIR0911853.1"
                     /translation="MTLLDCAVTGCAYNEDRCCCKGNIKVEGSEAVHQNETCCGSFVE
                     RGEKNCGCNVKEHESKTIDVACEACNCTFNENKKCSADHIGIAGGNACSCGETECASF
                     CCK"
BASE COUNT         1519 a         1232 c         1690 g         1354 t
ORIGIN      
        1 gacttttcgc ttgagataaa agaaggagaa tttttgattc tggttggacc gtccggctgc
       61 gggaaatcga cgctgctgcg catgatcgca ggtctggagg acatctcgtc gggcgagctg
      121 tgggtggacg gagagatcga gaattacaag gagccgcggg agcggaatct cgccatggta
      181 tttcagaatt atgcgctgta tcccaatatg agcgtgtacg acaatatcgc attttccctg
      241 agcgtgagga aggtcgataa gaaggagata aaaaagaaag tatacgatac ggcaaagatg
      301 cttggcattg agcatctgct ggacagaaga ccgggagaac tctccggcgg gcagaaacag
      361 cgtgtggcga tcggaaatgc gattatccgc aatccaaggg cgcttttgat ggacgagccg
      421 ctgtccaatc tggatgcgaa actgcggacg cagatgcgtg tggaactggc ggcattgcag
      481 catgcggtgg gaacaacgac gatctatgtc acgcacgatc agacggaggc catgacgctt
      541 ggcacacgga ttgtggtgat gcgggatgga aaaatgcagc aggtggatac accggcgaac
      601 ctctatgcaa agccggtcaa ccggtttgtc gcaggattca tcggaacgcc gtccatgaat
      661 tttttcgagg cacaagtggt ttcctgggag gaggctccgg cacttctggt cggggagtac
      721 cgcatgatgc cgtgcaggga ggatgcagcg cggataagca ggcacggaag cgacagcgtg
      781 atccttggca tacgcccgga aaacatcctg tgggaggcac cggaatgtgt aagcgccggg
      841 gcggattacg tggagacgat gattcttgag gtggagctgc tcggagccga gaagcttttg
      901 tttttccttt tatacgggaa aaagtatgtg gcaaagattc cggcggaata tgacgcccgg
      961 gcaggtgagg tgcgccggct gtacttcgat gtgacagcga tccatctgtt tgactgcgac
     1021 agtgaaatgc gcatctgaaa ggagacaaca tgaatgcatt tgtaaagcta agacaaaaga
     1081 tcagcccgta cctgtttttg gtgccgtgtc ttatcgtatt cggactgttc ttattttatc
     1141 cgtttgcaaa aacgatctat ttaagcctgt ataaaaccga caagctggga caggcaaagc
     1201 tgtttgtcgg cttcggaaac tatatcgatc tgtttacgtc agaatctttt tataacagcc
     1261 tgcttgtaac cctgattttt gtggtgatcg tggtaatggt cagcatgctg ctcggtcttg
     1321 tgacggcgct gctggtgaat aaaaattttc cgggaatccg ggtcttttcc acggcgtacg
     1381 cactgccgat ggcgatcgcc tcaagcgccg ctgcactgat tttcgagatt atgttagatc
     1441 cgacgatcgg cattctcgac aaattcctgc ggtcggacat caactggctg catgacgagc
     1501 gctatgcgct ggtgtgcgtg gcacttctga cggcgtggtt gaacagcggg atcaattatc
     1561 tgtatttttc ggcgggactt gccaatattg acgagtcgat ctatgagcgg gcgtctgtgg
     1621 atggcgcgaa cgagtggcag aagtttacca gattaacgct gccgggacta agcccgatcc
     1681 tgttttatac ggtcatcgtc aatattatcc tggcgttcca gtcgttcggg caggtgaaaa
     1741 tcttgacgga aggcgggccg ggtgaatcga cgaacctgat cgtctattcg atctaccgcg
     1801 acgcgttttt taatttccgc ttcggcgggg cggcggcaca gtctgtggta ctgtttttga
     1861 ttgtcatgct gctgacattg ctcatgttcc gtctggaaaa gaagggagtg aagtattgat
     1921 ggatatcagg ctcgagccgc gcactgcggc acatccgaag agagaagaga tcgaacggct
     1981 gcacgagacc atgaaaaaaa gggaactcgg cagaaaacgg cgcagattat ggagccgttt
     2041 cggcgtcaat ctggtggtgt cggtattggt gatccttccg cttttgtatg cgctttccat
     2101 tgcgtttatg ccatccgggg aattgttctc gatggagatg aatctgattc cgaagcagcc
     2161 gacgcttgac aattttgcac aggcgtttac aaagattccg ctgtttcgtt ttattttaaa
     2221 ttcgtttctg gtggcgggat gcattacgct cggacagatt atttcctgct cccttgcggc
     2281 gtttgcactg tcatttctgg actttaaggg aaaaaatgtg atttttatgc tgatcatggc
     2341 gacgatgatg gtgccgggag aagcgacgat tatttccaat tatttaacgg taagcggatg
     2401 gggctggctg aattcctaca aagtgctcat cattccatat atcacttccg ccatggggat
     2461 ctttttgttc cgtcagttct atctgacctt tccaaaatcc ctgtatgagg cggcaaagat
     2521 cgacggctgt ccgaatctgg tgttcattgt ccggattctg atctctctga cgaagtcggc
     2581 gatcggagcg atggcggtct ataccttcat caacgcttac aacatgtata tgtggccgtt
     2641 gttagtaacc ggaaccgagg agatgcgaac cgtgcagatt ggtatcagca tgttaaacag
     2701 tgtggattcc cagtcgatta cgatggtaat cgcgggagtt gtgatggtga ttctgccgtc
     2761 gctgtttgtg tttattctag gacaaaagca actgatccgc ggtatgtttt ccggtgccgt
     2821 caagggctga catagaaaaa aggaactcac taattcaaaa ggagaaaaaa gaggaatgaa
     2881 gaagagaatc attgcatcat tgttaacagc atccatgctt gcagcagcac ttgcaggctg
     2941 cgggggaagc acggcgcaga cggcggatac gtccgcagac gctgcagggg aagcggagag
     3001 tgcgcagggt gatccggcaa cggtgacgga aaaagagacg aatgtgtccg ccacggcaga
     3061 tgcggatctt tccgggacga cgatcacatt ctggcattct atgagcggtg taaacggcga
     3121 ggcacttacc gcgcttgtgg atcggttcaa tgaggagaac acctatggca ttacggtgga
     3181 tgcggagttt gagggcgagt acgatgatgc gatcaacaaa ttaaagagca gcatggtggg
     3241 aggaagcggt ccggatctga ttcaggtcta tgagatcggc acccgtttca tgatcgactc
     3301 cggctgggtg attccgatgc aggagctgat ggatgcgaac ggctatgaca actccaatat
     3361 cgtgccgaat attgccgctt actacacgat cgacgaccag ctctactcca tgccgtttaa
     3421 tacatccacg ccgattctct attacaataa ggatatgttt gatgcggcgg gaatcaccga
     3481 ggtgccgacg agcctggagg ggatcgatgc gattgccgac gatcttgtga acaagggcgg
     3541 tgcaggagag gcactttcca tgggaatcta cggatggttc tttgagcagt ttatctgcaa
     3601 gcagggactg gcctacgcaa acaatgacaa cggaagaagc gcggcggcga cagccgtgga
     3661 ttttgacggc aacggtgcgg ctctctcgat cgtatccgcc tggaaggatc tctacgacaa
     3721 gggatatgca ccgaatgtgg gagttggcgg cgatgcaggt ctgacagact tctctgcagg
     3781 aaaagcggcg attacgcttg gctccaccgc ttccttaaag cagatcttaa acgatgtcaa
     3841 cggctccttt gaggtgggaa ccgcatattt tccgggcatc aaggatacag atcagggcgg
     3901 tgtgtccatc ggcggagcat ccctctgggc aatccagaat caggatgatg taaaggcaca
     3961 ggcaacctgg aagttcgtgg aatatctggt atcggcagag tctcaggcat actgggcaac
     4021 ccagacagga tatttcccgg ttaccaacga tgcgtacaat gaggatgtat tcaagcagaa
     4081 catcgagcag tacccacagt tccagaccgc gatcgaccag ttgaacgaca ccaagggcga
     4141 gtacgcggga gcgcttctct ctgtatttcc ggaagcacgc cagacggtac agaccgagat
     4201 cgagaacacg ttaaatgaca aggagacacc ggaggaagcc gtacagaaga tggcggacac
     4261 gatcaacgcg tccatcgagg actacaatct gttgaacgaa tagtttttta gtggcaggca
     4321 atagggtaag cgacacatcc caccgatttc agatggaaat gcagatggaa cattttttga
     4381 tccgaatttc cagatacatt ccctctgaaa tcgaagggca tgatcttctc accctattgc
     4441 ctgccacgtc caaatgaacg atctgcttcc gtcgacgtcc cgttgctttt ggtgggttct
     4501 gtaaaaaagt ctttgaaata tgccggaaaa tttgttataa tactatagtg catctttgac
     4561 atagcagatg tcggacaagc aatccggcag atagatgcaa ataatcatga aatcgaggaa
     4621 tcagatatgg cattattaga gcagtggcag aaagttgctt ataatgagaa ggcagacaga
     4681 ggagaactgc agagattctg gcagagatat tttcttctgg aaaaaggtgt ctatgagaag
     4741 cttctgacga acccggatga gaaggtagag ggaacggtca aagagcttgc agataaatac
     4801 ggactgagca tcatggatat gacaggtttc ttggacggta tcaacgagag cctggtagag
     4861 ccgaacccga tcgacaccat ggaggaagat acgaaggtca gcctcgtatt cgacaaggag
     4921 aagttgtaca agaacatggt cgacgcgaag gcagactggc tctatgagct tccgatgtgg
     4981 aatgacattt ttgacccgga gacgaagaag agactgtatc tggagcagaa gaagtccgga
     5041 acggttatcg tcggaaagaa agttggacgc aacgatccgt gtccgtgcgg ttccggcaag
     5101 aaatacaaat tttgctgtgg aaagtaagca caggaagcag agcgcaaggg aagtaccggt
     5161 atgtggtact tccttttttg tgtaacagga aaattcttcc tgctgtggaa aaagaaaacc
     5221 ggttataaaa gaaattgctc cggagatact taagaatggg aatgtatcat tccagagaac
     5281 aaggatcgaa aggagaaaag agatatgaca ttactggatt gtgcggtaac cggatgtgct
     5341 tacaacgagg atcgctgctg ctgcaagggc aacatcaagg ttgagggaag cgaggcggtg
     5401 catcagaatg agacctgctg cggaagcttt gtggagcgcg gggagaaaaa ctgcggatgc
     5461 aatgtaaaag agcatgagtc aaagacaatc gatgtggcat gtgaggcctg caactgcacg
     5521 tttaacgaga ataagaaatg tagcgcggac catatcggca tcgcaggggg caacgcctgt
     5581 tcctgcggcg agacagagtg cgcaagtttc tgctgcaaat aggagaaaag acggggagcc
     5641 ggaggcaatg caaaacggtt ccccgttttg cattccggtg gaattttcct cttgtaaagt
     5701 gcgtctgatg gaagtacaat gaaagcaaaa ttaggaaaaa agtcatgcag tgcagaaggg
     5761 ggaaccgatg aatgagaaaa aagtcacata tttcg
//
LOCUS       DBMDQJ010000054         5761 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5568_length_5761_cov_1.629859, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000054 DBMDQJ010000000
VERSION     DBMDQJ010000054.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5568_length_5761_cov_1.629859"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /gene="sdaAA"
                     /locus_tag="ACZO9R_01870"
     CDS             <1..866
                     /gene="sdaAA"
                     /locus_tag="ACZO9R_01870"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HIR0911854.1"
                     /translation="SLEELCRMEKESGKPFWKIVQEDDCVEQGISAEESFAQMSGMYQ
                     AMQSADAHYDETLKSASGLVGTEGTKMKRAREAGTLLCGDFIGKVMEKALKTSESNAC
                     MKRIVAAPTAGSCGVVPAVFLSVQEERGFSEDRMVEALYVAAGIGGVIANRAFLAGAA
                     GGCQAEIGSASAMAAGGVAYLLGGGADEIANAAALALKNLLGLACDPVAGLVEVPCVK
                     RNVMGAVNAMTSADMTMAGIFSKIPPDEVIDAMRSIGRSMNEDIRETGKGGLAGTPTG
                     VEIRDRMAGTL"
     gene            complement(1484..2824)
                     /locus_tag="ACZO9R_01875"
     CDS             complement(1484..2824)
                     /locus_tag="ACZO9R_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyryl-CoA:acetate CoA-transferase"
                     /protein_id="HIR0911855.1"
                     /translation="MDFREEYKQKLVSADEAVKLIKSGDWVDYGWCTNTVDALDQALA
                     KRTDELTDVKLRGGILMKPLAVFAREDAGEHFCWNSWHMSGIERKMINRGVAYYCPIR
                     YSELPRYYRELDCPDDVAMFQVAPMDAHGYFNFGPSASHLGAMCERAKHIIVEVNENM
                     PRCLGGTECGIHISDVTYIVEGSNPPIGELGAGGPATDVDKAVAKLIVDEIPDGACLQ
                     LGIGGMPNAVGSLIAESDLKDLGVHTEMYVDAFVDIAKAGKINGSKKNIDRYRQTYAF
                     GAGTKKMYDYLDDNPELMSAPVDYTNDIRSISALDNFISINNAVDIDLYGQVNAESAG
                     IKQISGAGGQLDFVLGAYLSKGGKSFICLSSTFKTKDGQVQSRIRPTLANGSIVTDAR
                     PNTHYVVTEYGKVNLKGLSTWQRAEALISIAHPDFRDDLIKEAEQMHIWRRSNR"
     gene            complement(2937..4007)
                     /locus_tag="ACZO9R_01880"
     CDS             complement(2937..4007)
                     /locus_tag="ACZO9R_01880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0911856.1"
                     /translation="MATISLCMIVKNESAVLARCLDSIADLMDEIIIVDTGSTDNTKE
                     IAAQYTSRIYDFKWASDFSAARNFSFSKATMEYIYTADADEILDDANRERFLRLKDAL
                     LPEIEIVQMKYVTQGTFDTVLNAKKEYRPKLFRRLRTFTWIDPIHETVRLTPVTFDSD
                     VEILHCPQSLHSKRDFSIFIKAFEHDGQFSPKIRTMYAKELLKTGDKKDFTDAKPIFQ
                     LIFDNDPADDARKEAACVLARIYRMEDNKNEFFKLTMKDMLTTPCAEICYELGTYFVG
                     QRDYAEAVLWFYNAAYETESILDVHTSGDLPLLGLVECYETLLAGEEAKIPSDTALTI
                     QYEMMLDKYREASRDWRMPEET"
     gene            4257..4415
                     /locus_tag="ACZO9R_01885"
                     /pseudo
     CDS             4257..4415
                     /locus_tag="ACZO9R_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
     gene            4431..5630
                     /locus_tag="ACZO9R_01890"
     CDS             4431..5630
                     /locus_tag="ACZO9R_01890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR0911857.1"
                     /translation="MSAIVTLKKGEGRTIKAGGMWVFDNEIDTVTGTFENGEVVLVHD
                     FDGYPMGRGFINVNSKIHVRMLTRKIDQEIDRAFLQMRVQNAWDYRKTTVDTSSCRVI
                     FGEADFLPGLVVDKYEDVLVVECLALGMEQFKETIVELLKETLAQDGICIRGVYERSD
                     ANERTKEGLPKVKGFIGAEFDTNVEIVENGVHYMVDVVNGQKTGFFLDQKYNRLAMQR
                     ICKGKKVLDCFTHMGTFALNAGIAGAAEVTGLDISGYAVEQARENARRNGLEDTVQFR
                     CANVLDELPKLAQSGEQYDVVILDPPAFTKSRQATKNAIKGYREINMKGLKLVKDGGY
                     LATCSCSHFMTQELLAKTIREAARSAHKRLRQVEFRTQAPDHPILWAADESYYLKFYI
                     FQVVDEK"
BASE COUNT         1365 a         1306 c         1719 g         1371 t
ORIGIN      
        1 gatcattgga ggaactgtgc cggatggaga aggagtccgg gaagcctttt tggaaaattg
       61 tgcaggagga tgactgcgtg gagcagggca tcagcgcaga ggagtcgttt gcacagatga
      121 gcggaatgta tcaggcgatg cagtctgcgg atgcacatta cgatgagacg ttaaaatcgg
      181 ccagcggact cgtcggaacg gagggaacca agatgaagcg ggcgcgggaa gcgggaacgc
      241 tgctctgcgg agactttatc ggaaaagtga tggagaaggc gttgaaaacc agtgagtcca
      301 atgcatgcat gaagcggatc gtggcggcac cgactgcggg ctcctgcggt gtggtaccgg
      361 cagtgttttt gagtgtgcag gaggagcgcg gtttttcaga agatcgcatg gtggaagctt
      421 tgtatgtggc agcgggaatc ggcggcgtga ttgcaaaccg ggcgtttttg gcgggcgcag
      481 cgggcggctg ccaggcggag atcggttcgg cttccgcgat ggcggcaggc ggtgtggcgt
      541 atcttctggg cggcggtgcg gatgagattg ccaatgcagc ggcgctggcg cttaagaacc
      601 tgcttggact ggcatgcgat ccggtggcgg gacttgtaga agttccgtgt gtgaagcgta
      661 atgtcatggg agccgtgaat gccatgactt ccgcagatat gacgatggcg gggattttca
      721 gtaagattcc gccggatgaa gtgattgacg cgatgcgcag tatcgggcgc agcatgaatg
      781 aggatatccg tgagaccgga aaaggagggc ttgcggggac accgaccgga gtagagatcc
      841 gcgaccggat ggctggaacc ctgtagacaa ccggttagtg ccggaggacg gctgacagat
      901 gggaagtgga atggtaacag gattgacagg atctgaaggg gaagaatcat cctgttccag
      961 aatggaagca ggcgtggaga atcgttggag caaaatacgt gaatttcttg catgccgctt
     1021 ttgacattat attccgtgtc tttgccacgt ttggacaatc cacaggaggt gtccggcaaa
     1081 ccgcagacaa tgggcggact ttctggtgtc cctaagggca ccgcttcaca gacaatataa
     1141 aaccgcgcga accgaaactc gttggcatct gctattgaaa ataaggcaga tgccaattca
     1201 aacatcggct tcgcgcggtt atgttctgtt cagcggtgcc cataagggac acacacaaaa
     1261 gtcctaaaat tgtctgcagg ttgcgaacac cttctgcgga gtgttccaaa cttggaaaga
     1321 catggatcat cgaagatgtc aaaagcggca tgcttagaaa ttccgtattt ggcgattgga
     1381 ttctccacgc ctgctttcat ctggaacatg gctgttcttc tggcctcaga tagtgaaact
     1441 atatgcaaca ttccacgtcc cgtccgtcag tcgtcctccg gtactagcgg ttgcttcttc
     1501 tccagatgtg catctgctcc gcctctttga tgaggtcgtc gcggaaatca ggatgcgcga
     1561 tcgagatcag agcctcggct ctctgccagg tagacagacc ctttaagttc accttgccgt
     1621 attcggttac aacatagtgt gtattcggtc ttgcgtcggt aacgatggaa ccgtttgcca
     1681 gcgtcgggcg gatacgggac tgcacctgac cgtccttggt cttgaaagta gaggataagc
     1741 agataaagct cttgccgccc ttggacagat aagcgccgag cacgaagtca agctgtccgc
     1801 ctgcgccgct gatctgcttg atgcctgcag actctgcatt tacctgacca tagagatcga
     1861 tatccacggc attgttgatg gaaataaagt tatccagtgc ggagatcgag cggatgtcgt
     1921 tcgtgtagtc gaccggtgcg ctcatcagtt ccgggttgtc gtccagataa tcgtacattt
     1981 tcttggtgcc ggcgccgaaa gcgtaggtct ggcggtaacg gtcgatattc tttttggaac
     2041 cgttgatctt acctgccttt gcaatatcga caaatgcatc cacgtacatc tcagtgtgaa
     2101 cgccgagatc cttcaagtcg gactctgcaa tcagggaacc gacagcgttt ggcatgccgc
     2161 cgataccgag ctgtaagcag gcaccgtctg gaatctcatc gacgatcagc tttgcgacag
     2221 ccttatccac atccgtagca ggaccgcctg cacccagttc accgattggc gggttggagc
     2281 cttctacgat gtaggtgaca tcggaaatgt ggatgccgca ctcggtaccg ccgaggcagc
     2341 gtggcatatt ttcattgact tctacgatga tgtgctttgc gcgctcgcac attgcaccca
     2401 gatgtgaggc actcggaccg aagttaaagt agccgtgcgc atccatcgga gcgacctgga
     2461 acatggcaac gtcatccggg cagtcaagct cgcggtagta gcgcggcagc tcggagtagc
     2521 ggatcggaca gtagtaagcc acgcctctgt ttatcatctt gcgctcgata ccggacatat
     2581 gccaggagtt ccagcagaaa tgctcgcctg catcctcacg tgcaaaaaca gccagcggct
     2641 tcatcaggat accgccgcgc agcttgacgt ctgtcagttc gtcggtgcgc tttgcgagag
     2701 cctgatccag tgcgtcaacg gtgttggtgc accagccgta atctacccag tctccggatt
     2761 tgatgagctt taccgcctca tctgcggaga caagcttctg tttgtattct tcacgaaaat
     2821 ccatctcaaa tacctcccac acaaaagatt gttaaaattt taccatagtt tcatcctatc
     2881 actttatgtt gtattttaca agccctgccg gagcgtattt ccggagattt gtaagatcag
     2941 gtctcctccg gcatccgcca gtctctggaa gcctcccggt atttgtcgag catcatttca
     3001 tactggatgg taagcgctgt gtcggaagga attttggctt cctccccggc gaggagcgtc
     3061 tcgtaacatt cgacgagacc gagcagcgga agatccccgc ttgtgtgaac gtccaggatg
     3121 ctttccgtct cgtaggcggc attgtagaac cagagaaccg cttccgcgta gtcgcgctgt
     3181 cccacaaaat aggttcccag ttcgtagcag atttctgcgc acggtgtggt gagcatgtcc
     3241 ttcatggtca gcttgaaaaa ttcgttcttg ttatcctcca tgcggtaaat ccgggctagg
     3301 acgcaggcag cttcctttcg ggcatcatct gccgggtcat tgtcgaaaat gagctggaag
     3361 atcggttttg catcagtaaa atcctttttg tcgcccgttt ttaaaagctc tttggcatac
     3421 atggtgcgga tttttgggga aaattgcccg tcgtgttcaa acgctttgat gaagatggaa
     3481 aaatcgcgct tgctgtgcag ggactgcgga cagtgcagaa tctccacatc gctgtcaaaa
     3541 gtgaccggcg ttaagcggac ggtctcgtga atcggatcga tccaggtaaa cgtgcgcagt
     3601 ctgcggaaca gctttggccg gtattccttt tttgcattca aaacggtatc gaacgtgccc
     3661 tgcgtcacat acttcatctg tacaatctcg atctcaggca gaagggcatc tttaagacgg
     3721 agaaaacgct cgcggtttgc gtcgtcgaga atttcatcgg cgtcggcggt gtagatatat
     3781 tccatcgttg ctttggaaaa ggaaaaattg cgtgcggcgg aaaagtcgct ggcccatttg
     3841 aaatcgtaga tccgcgaggt gtactgtgcg gcaatttctt tcgtgttgtc ggtggagccg
     3901 gtgtctacaa tgatgatctc gtccatgaga tcggcgatgc tatccagaca gcgcgccagc
     3961 actgcggact cattttttac aatcatacat aaactgatag ttgccatagt ttctcctttg
     4021 tgggaggcag tgatatgccg ctgccccgtg cgtgataact tctatcctaa gtataacgca
     4081 gcgggggatg gcaggcaaac aggaaaataa attgtcgtgg caagatggaa ggttctgtgc
     4141 taaaatgagc ggtggttatg tctgcagggc ggacggaagg acagatggaa acggagtata
     4201 cagataaatg agacaacaat ttatgcagaa gacgatcgtc gtgtggcagc ccgggaaatg
     4261 ttgcagtctt agggtttatg aatccggtgt gcggtgtgat cctgtccgcg gttctgctcg
     4321 gtgagacgga cagcttaagc gtgatgtcgg tggctgcgct ggtgctggta tgcatgggaa
     4381 tttacattgt aaaccgcccc aaaaaggcgg aatagaaaaa ggaggaacct atgagcgcga
     4441 ttgttacatt gaaaaagggt gagggaagaa ccatcaaagc cggaggaatg tgggtgttcg
     4501 acaatgagat cgacacggtg accggaacat ttgagaacgg cgaagtggtg ctggtacacg
     4561 attttgacgg ttatccgatg ggaagaggtt ttatcaacgt caattccaag atccatgtcc
     4621 ggatgctgac aagaaagatc gatcaggaaa tagaccgcgc atttttgcag atgcgtgtgc
     4681 agaatgcatg ggattaccgc aagacgacgg tggacacctc ctcctgccgt gtgattttcg
     4741 gggaggcgga tttcctgccg gggctggttg tagataaata tgaggacgtt ctggtcgtgg
     4801 agtgtctggc gcttggaatg gagcagttca aggagacgat cgtggaactt ttaaaggaga
     4861 cacttgcgca ggacgggatc tgcattcgtg gcgtttacga gagaagcgat gcgaacgagc
     4921 gcacgaagga aggcttgccg aaggtaaagg gatttatcgg agcagagttt gacacgaatg
     4981 tggagattgt ggagaatggc gtccattata tggtagacgt tgtgaacgga cagaagaccg
     5041 gattttttct ggatcagaag tataatcgtc ttgccatgca gcggatctgt aaagggaaaa
     5101 aggttctgga ctgttttacc cacatgggaa cgtttgcctt aaatgcagga attgcgggcg
     5161 ccgcagaagt cacgggtctt gatatttccg ggtatgcggt ggagcaggcg cgggagaatg
     5221 cgcgccggaa cggactggag gacacggtgc agttccgctg tgccaatgtg ctggatgaac
     5281 tgccgaagct ggcacagagc ggcgagcagt acgacgtcgt gattcttgat ccgccggcat
     5341 tcacgaagtc gcgtcaggcg acgaaaaatg caattaaagg ttatcgtgag attaacatga
     5401 aaggtctaaa gcttgtgaag gacggcggct atcttgccac ctgctcgtgt tcacacttta
     5461 tgacacagga gctgctcgcc aagacgatcc gcgaggcggc acgctcggcg cacaagaggc
     5521 tgcgccaggt cgagttccgc acgcaggcac cggatcaccc gattctgtgg gcggcggatg
     5581 agagttatta tctgaagttc tacatttttc aggtcgttga cgagaagtaa gggctggaaa
     5641 gtggcggaaa tgcttgattt acaagggttg gaaggtgttt ggtgattagc ttgcaaacct
     5701 cgtaaaatat cgcaaaatat cgtatcacag tggtataaag tggtaagaaa agtggtaagt
     5761 c
//
LOCUS       DBMDQJ010000055         5730 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5601_length_5730_cov_1.630663, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000055 DBMDQJ010000000
VERSION     DBMDQJ010000055.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5730)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5730)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5730
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5601_length_5730_cov_1.630663"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..598)
                     /locus_tag="ACZO9R_01895"
     CDS             complement(<1..598)
                     /locus_tag="ACZO9R_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme"
                     /protein_id="HIR0911858.1"
                     /translation="MAGLGHRQDRGVMLPPLDEIDGFMVRPGFYNSEGAVPTARGVSF
                     TIHSVGATGCTLLLFRPQEKEPYARLKYPEAYHIGNTFAMLVFGLKIDEFEYAFQLDG
                     PYDESRGLLFDKNNVLLDPFAKAVTGQRNWGERPESDEGFVYHARVVENNFDWGKMTF
                     PEIPAEDLIIYETHVRGFTRDASSGVTAGGTFEGLRQKI"
     gene            796..1302
                     /locus_tag="ACZO9R_01900"
     CDS             796..1302
                     /locus_tag="ACZO9R_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR0911859.1"
                     /translation="MHTFQPLPFDLVEFNPFTKIGKEWALVTAGSREKANTMTVSWGG
                     VGVLWGKNVAFIFVRDSRYTKEFIDANEFFSITFLSGQYRDALNYCGSHSGRDEDKFA
                     AAGLNLATRHNIPYVDEGNFVLLCEKMSATRITEDSFLLPEIKEKWYKDGDMHTMYVA
                     EIIDILAR"
     gene            1331..2300
                     /locus_tag="ACZO9R_01905"
                     /pseudo
     CDS             1331..2300
                     /locus_tag="ACZO9R_01905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081027.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pectinesterase family protein"
     gene            complement(2556..3152)
                     /locus_tag="ACZO9R_01910"
     CDS             complement(2556..3152)
                     /locus_tag="ACZO9R_01910"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911860.1"
                     /translation="MRLKAEHISYKYPGGSREVLSDVSLELGSTDRLGIAAPSGFGKT
                     TLCQILAGYRTPTTGRVCVDDMPLPGSGYCPVQMIWQHPEQAVNPRVRMQAVLQEGDS
                     ISERLIEGLGIERDWCNRYPGELSGGELQRFCIARALGERTRFLIADEISTMLDPITQ
                     SQIWNFLLAEVKERKIGLLVVSHDEKLMEQVCDRIMRL"
     gene            complement(3185..4198)
                     /locus_tag="ACZO9R_01915"
     CDS             complement(3185..4198)
                     /locus_tag="ACZO9R_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911861.1"
                     /translation="MEEKKELLRIEHLTVSFAQYEKRSAKQGELPVIRDLNVTVREGE
                     IVAVVGSSGSGKSLLAHAIMGILPANAAASGSIFYRGSACGEAELKALRGREIALVPQ
                     SVNYLDPLMKVGRQIIGETKKRQKLFCGADQGKRQKLFCGADREKRQEEARMRELLAR
                     YGLADEVADLYPFELSGGMARRVLLLTALMWQPRLIIADEPTPGMDLALAKQAMQDFR
                     TFADDGNGVLLITHDLELALEVADRIVVFYAGTTVEEAKVTDFADETLLRHPYTRALY
                     EALPGRGFAALPGTQPYVKDLPAGCPFAPRCADRREACDGEIPVVCVRGGRVRCILYT
                     ENS"
     gene            complement(4221..5084)
                     /locus_tag="ACZO9R_01920"
     CDS             complement(4221..5084)
                     /locus_tag="ACZO9R_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0911862.1"
                     /translation="MDKTGHMTIAAHTAGRRNGRQWMRRFLLAAVLLLAAVAVAGICL
                     EERAVVTDFSRKNLPPCLKYLFGTDWMGRDMLSRTLAGLSTSIRIGVLASAVSAVLAL
                     FLGTAAAVGGKWADGIISWCIDLMMGVPHIVLLLLISYALGKGEKGVVVGVALTHWPN
                     LARVIRGEILQVREAAYVGIAKQLGKSPWYIARWHIFPQILPQFLVGLVLLFPHAILH
                     EASITFLGFGLPPEKPAVGVILSESMSYLSTGKWWLALFPGLALVGVVVLFDRIGSGL
                     KALFDPSSAQL"
     gene            complement(5077..>5730)
                     /locus_tag="ACZO9R_01925"
     CDS             complement(5077..>5730)
                     /locus_tag="ACZO9R_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0911863.1"
                     /translation="VLSGILGLVLGIVAGYFKNRLPDHLIRGYALLLASTPAFWIGLL
                     LLMIFAVWLGWLPFGMNLPAGMTAAEAGIGVRIRHLILPALTLGLTGVANITLHTREK
                     MIAIMESDYVLFARARGESSGYIVKRHALRNLVLPAITLQFGSISEIFGGSVLVEQVF
                     SYPGLGQAAIDAGLGSDVSLLLAITVISTLFVFLGNLAANVLYGVIDPQIRRGRSHG"
BASE COUNT         1258 a         1860 c         1359 g         1253 t
ORIGIN      
        1 gaatcttctg gcgcagtccc tcaaacgtgc cgcctgccgt caccccggaa gaagcgtccc
       61 tggtaaatcc tcttacatgt gtctcgtata tgatcagatc ctccgccgga atctccggga
      121 acgtcatttt cccccagtca aaattattct ccaccacacg ggcgtgatac acaaagccct
      181 cgtcactctc cgggcgctcg ccccagttcc gctgccccgt caccgccttc gcaaacggat
      241 ccagcagcac attgttcttg tcaaacaaca gccccctgga ctcatcatac ggtccgtcca
      301 gctgaaacgc gtattcaaat tcatcaatct tcaagccgaa taccagcatt gcgaacgtgt
      361 tgccaatgtg gtatgcctcc ggatacttca gtctggcgta tggctccttt tcctgcggcc
      421 ggaacagcag aagcgtacat cccgtcgcgc cgaccgaatg aatcgtaaag ctcactcctc
      481 tcgccgtcgg cactgcgcct tctgaattgt aaaatcccgg gcgcaccata aaaccgtcaa
      541 tctcatccaa tggcgggagc atgactcccc gatcctgtct gtggcctaat cctgccatcg
      601 ccgctcctcc ttcttccctt tgcgtactat attactatta tacgcactcc gtctattttt
      661 tcaactgctc tggcgctcat ctgcgcccct tccggtgtga aaagtatccc gttttgccgc
      721 cggctttctt gtcattttcc cgtcaaaatg gtatcatatc tacagaaatg ataccgacag
      781 aaaggaagta atcttatgca cacattccaa ccgttaccat ttgatcttgt agaatttaat
      841 ccatttacca aaatcggcaa agagtgggcg cttgtcacag ccggcagcag ggaaaaagcc
      901 aacaccatga ccgtaagctg gggcggtgtc ggtgtcctct ggggcaaaaa tgttgctttc
      961 atttttgtgc gtgattcccg ctacaccaaa gaattcatcg acgcgaacga atttttctcc
     1021 attaccttcc tgtccgggca ataccgtgac gcattaaatt actgcggttc ccattccgga
     1081 agagacgaag acaaattcgc cgcggcaggt cttaacctcg ccacgcgcca caacatccct
     1141 tacgtggacg agggcaactt tgtccttctc tgcgagaaaa tgtctgccac ccgcatcacg
     1201 gaagactctt ttcttctccc tgagatcaag gaaaaatggt acaaggacgg cgatatgcac
     1261 accatgtatg tcgcggagat catcgatatt ctcgcccgtt aattctattt ttccaaaaag
     1321 gaagccactt atgtatacaa tgaccgttgc gccaggggca gatgtctctg gcggctttta
     1381 ttcactgaaa gcggcgttcg ccgccatgcc ggaagatccg gcggttcccg tgaccatccg
     1441 cgtcatgccc ggaatctacc acgaaaaatt atccctcatg cgcccgaacg tcacgatcga
     1501 gggcgcagga gcatccccgt ccgacaccgt gatctccttc ggcgattacg gctacgagat
     1561 catgccggac ggcatcaagc gcggaacctt ccgctcctac acctttttcg tgcatgccgc
     1621 agacgtcacg ctgcgcaact taaccatcga gaatactgcc ggcgactcca aaacgcacgg
     1681 gcaggcaatt gcactctatg ctgaatgtga ccgttttgtc gcggaatact gccggattct
     1741 cgggcatcag gatacgcttt ttaccggtcc gcttcccccg gaggaatacg agcccggcgg
     1801 ctttcgcggg ccgaccgagt ccgcgccgcg catcaacggc agacaatatt atcataactg
     1861 ctatatctgc ggcgatatcg actttatttt cggcagtgcg accgcttact ttgaaggctg
     1921 tacgatcgca tccttaggac ccggatatgt caccgccgct tccacccccg aagggcagga
     1981 atacggctat gttttccacg actgccgctt tgccgcggag ggttgtcctc agcacgcggc
     2041 ctatatcggc cgtccctggc gggattacgc acgggttgtc ctcatggact gcgcgatagg
     2101 cgcccacatt catcctgccg gatttcatga ctggggcaag gagcacgcac acggcaccgt
     2161 tctttttgcc gagtatcaca gttatccaca ggacgctgac tgtggctgtg cagacagctg
     2221 ccgcccgttt gcacagcgca caggctgccc atttttccag agagcttgtg ctggcggggg
     2281 acgacggctg gctgccgtga ccgttttctt gtatattccg ccaaatgatg gtaaaatact
     2341 aaccataaat ggattgtttt gtattaagga ggttgttttt atggaagaaa agatttacaa
     2401 gacaatggga tcgactggcg ctgcaagcct tgccgtcggc atctgcgtac tgacaggcgg
     2461 cattgcagcc ggaattcttc tcattgtgaa tggcgcgcgc ttattgaaga accgcgcgaa
     2521 gatgatattc tgatttttct ttttatttgc aggcatcaca gccgcatgat gcggtcacag
     2581 acctgctcca tcagcttttc gtcgtggctt acgaccagaa gccctatttt tcgttctttc
     2641 acttccgcca gcaggaagtt ccagatctgg ctctgggtga tgggatctaa catggtgctg
     2701 atctcatctg caatcaaaaa ccgtgttctc tcccccagag ccctcgctat gcagaatcgc
     2761 tgcagttctc ctccggatag ttcgcccgga taccggttac accagtcccg ctcaatcccg
     2821 agcccctcta tcaaacgttc gctgatcgaa tctccctcct gcagaaccgc ctgcatccgc
     2881 acgcgcggat tcaccgcctg ctccggatgc tgccagatca tctgcaccgg gcagtaaccg
     2941 ctccccggaa gcggcatatc gtccacacag acacgtccag tcgtcggtgt gcggtagcct
     3001 gccaggatct gacagagcgt cgtcttacca aagccgctcg gcgcggcaat ccccagccgg
     3061 tcggtactcc ccagttccag actcacatcc gacagcacct ctctgctgcc ccccggatat
     3121 ttatagctga tatgttccgc tttcagtctc accggacgtt tcctcctctc cctctgatag
     3181 ttctttatga attctctgta tacaatatgc agcgcaccct gccgccgcgg acacaaacga
     3241 ccggaatctc cccgtcacac gcctcccgac ggtcggcgca gcgcggtgca aacggacatc
     3301 ccgccggcag atctttcacg taaggctgtg ttccgggcag tgccgcaaac ccgcgccccg
     3361 gcagcgcctc atacagcgct ctcgtatatg ggtgccgcaa cagcgtctcg tccgcaaaat
     3421 ctgtcacctt cgcttcctcc acggtcgtcc cggcataaaa cacaacgatc cggtccgcca
     3481 cttcaagcgc cagttccaga tcatgtgtaa tcagaagcac accgtttcca tcgtccgcaa
     3541 atgtccggaa atcctgcatc gcctgttttg ccagcgcaag atccatgccc ggcgtcggct
     3601 cgtccgcaat gatcaggcgc ggctgccaca tcagtgccgt cagtaaaagc acacggcgcg
     3661 ccatgccacc ggatagttca aacggataga gatccgcaac ttcgtccgca agcccatacc
     3721 ttgcaaggag ttcccgcatc cgcgcttcct cctgccgctt ttcccggtct gctccgcaaa
     3781 aaagcttctg ccgctttccc tggtctgctc cgcaaaaaag cttctgccgc ttcttcgttt
     3841 ccccaatgat ctgtctgccg accttcatca gaggatcaag ataattgaca ctctgcggca
     3901 ccagcgcgat ctccctgccg cgcagcgcct ttaattccgc ctcgccgcag gcgcttcccc
     3961 ggtaaaaaat actgccgctt gcagccgcat tggcggggag aattcccatt atggcatgcg
     4021 ccagaaggct ttttcccgat ccgctgctcc cgacgaccgc gacgatctca ccctcacgca
     4081 ccgtcacatt cagatcacgg atcaccggaa gttccccctg ctttgcgctg cgcttctcat
     4141 actgcgcgaa agataccgtc agatgctcga tgcgcagtaa ttcttttttc tcttccatcg
     4201 ttttctccac tcaaaatctt ctacagctgc gcactcgacg ggtcaaacag cgcctttaag
     4261 ccgctcccga tccggtcgaa aagaaccacc acgccgacca gcgccagtcc cgggaagagc
     4321 gccagccacc attttccggt gctcagatac gacatactct ccgagaggat cacgccaacc
     4381 gccggcttct ccggcggcag tccaaaaccc agaaacgtga tgctcgcctc gtgcaggatc
     4441 gcatgcggga acagtaacac cagccccaca agaaactgcg ggagaatctg tggaaaaatg
     4501 tgccatctgg caatgtacca cggacttttc ccgagctgtt ttgcaatgcc aacgtacgcc
     4561 gcctcccgca cctgcaggat ctccccgcgg atcacgcgcg cgaggttcgg ccagtgtgtc
     4621 agcgccacac cgacaaccac acccttttca ccctttccaa gcgcgtagga aatcaatagc
     4681 aacagcacaa tatgcggaac tcccatcatc agatcaatgc accagctgat gatgccgtcc
     4741 gcccattttc cgccgaccgc cgcggcagtt ccaaggaaaa gcgcaagcac tgcgctgacc
     4801 gccgacgcca gcacaccgat gcggatgctc gtggagagcc ccgccagcgt gcgcgacagc
     4861 atatcacgcc ccatccagtc cgtcccaaac agatacttaa ggcacggcgg cagatttttg
     4921 cgcgaaaaat ccgtgaccac cgcccgctcc tccagacaga ttccagccac tgcaacggct
     4981 gctaaaagca gcactgccgc cagaagaaag cgccgcatcc actggcgccc gttgcgtctg
     5041 cccgccgtgt gggcagcaat cgtcatatga ccggttttat ccatgacttc tgcccctcct
     5101 tatctgcgga tcaatcacac cgtaaagtac atttgccgca agatttccga ggaatacgaa
     5161 cagtgtgctg atgaccgtga tcgccaaaag cagcgacaca tcgctcccca gcccggcatc
     5221 aatcgctgcc tgtccgagtc ccggataaga aaatacctgc tccacgagca ccgagccgcc
     5281 aaagatctca ctgatggaac caaactgcag ggtgatggca ggcagcacca gattgcgcag
     5341 ggcatgtctt tttacgatat agccgctgct ctccccgcgc gcgcgcgcaa acagcacata
     5401 gtcgctctcc atgatcgcaa tcattttttc ccgggtgtgc agcgtgatgt ttgccacgcc
     5461 cgtcagcccg agcgtcagcg ccgggagaat cagatgccgg atgcgcacgc cgatgcccgc
     5521 ctccgccgcc gtcatgcctg ccggcagatt catgccgaac ggcagccagc cgagccacac
     5581 ggcaaagatc atcaagagca gcagaccgat ccagaatgcc ggcgtgctag ccagaagcag
     5641 cgcatacccg cggatcagat ggtccggcag acggttctta aaataccccg ccacaatgcc
     5701 gagcacaagc cccaggattc cggacaatac
//
LOCUS       DBMDQJ010000056         5716 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5619_length_5716_cov_1.803028, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000056 DBMDQJ010000000
VERSION     DBMDQJ010000056.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5619_length_5716_cov_1.803028"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="ACZO9R_01930"
     CDS             <1..626
                     /locus_tag="ACZO9R_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HIR0911864.1"
                     /translation="LEEISKMKEKNLAVELLKKLISEQVQIYRHTNIVKSQKFSEIIQ
                     RAMNAYLNGMLTNEQVIEELLNLAKQIAAANKEGEQLGLTADELAFYDALTKPQAIKD
                     FYENAELIAITKELADTLRKNRTIDWQKRDSARAKMRIMIKRLLKKHRYPPEGMDDAV
                     QTVMMQCELWTDNNDMESEQKNINVYTYSSKPELSRVAEKPAPYGSR"
     gene            799..2727
                     /locus_tag="ACZO9R_01935"
     CDS             799..2727
                     /locus_tag="ACZO9R_01935"
                     /inference="COORDINATES: protein motif:HMM:NF018584.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HIR0911865.1"
                     /translation="MAVGLCHSDKKSVCRNLQYNENVNMPDSPGWKWVQTVQTEAADR
                     YLIRLKSREKGRARKTYRVEVKEDAVNAKSAPNVEQIVLLLLLLPLLGIIFAGLRYSL
                     TAENPQDDLQLVLILTVIAGILGLRIWLIQWKADKTLRYFLMTQIINAPGNRNGAYAG
                     AAQRTADQTWGRTAYAQDAAYADDAAYADEGIAGLSEMEEPEERVWVAEWHLSEAYRR
                     YSRLQAIPLAALALGGIIFLYAIAYRFNTSHDTARTMLLLGEAGLYMTLLALLSKVME
                     YCYLKKAKAQAKRYHARIARVITTGKNGANSYYLYEFVDELGRKCEVRAMYPVRPANA
                     ANAQDMAGGEADIWFDVEHFPYAFSEKDGAPEIRLKLRSMIAGTAVAAVVIALVFAYG
                     GTHYVAYNQDADSLLMSYDSKKAESDLTFEDVRDNPTVRWIIDAYAIYVPFESGEYGE
                     LGGHSLNDWDQEQVKEYLSDWWGITSRATATRTISQMLKKGTRASYRHAFETYLKKGY
                     LSMDENGYVDIISISEIPEDEQCRTWVCYDAYGHLDTRGVDAWDYVRIMRITGLCYQC
                     GYISLEECLDQCLPIAQRLQKEYGSFEEIFESYIYGYQFWKNDSDDDRIYFYRRAAGE
                     AVENIQSEYNTELVKDWE"
     gene            2737..3789
                     /gene="rsmH"
                     /locus_tag="ACZO9R_01940"
     CDS             2737..3789
                     /gene="rsmH"
                     /locus_tag="ACZO9R_01940"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR0911866.1"
                     /translation="MEQETQTPHKRRVRYKGKYPKKFEEKYKELQPEKYQDTIEHVIR
                     KGNTPAGMHISIMVKEILDFLQIVPGEQGFDATLGYGGHTRAMLECLKGQGHIWATDV
                     DPEESAKTKKRLEDLGFGEDLLTVRLQNFCTIDEIAKEVGGFDFILADLGVSSMQIDN
                     PKRGFSFKVDGPLDLRLNQETGISAAERLDTITREELAGMLYENSDEPYCEELAKAIT
                     DEIRRGSRIDTTTKLRRVIEDTLDFLPEKEKKDTIKKTCQRTFQALRIDVNQEFEVLY
                     EFMEKLPGALRPGGRVAILTFHSGEDKLVKQALKEGYKSGIYSDYAKDVIRPSAQECA
                     ANGRARSTKMRWAVRA"
     gene            3838..4821
                     /gene="bsh"
                     /locus_tag="ACZO9R_01945"
     CDS             3838..4821
                     /gene="bsh"
                     /locus_tag="ACZO9R_01945"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HIR0911867.1"
                     /translation="MCTAATYQTKDFYFGRTLDYEFSYGEEIVITPRNYEFNFRHMGQ
                     AEEHYAIIGMAHVAGDYPLYYDAINEKGIGMAGLNFVGNAVYAEVTEGKENVASFEFI
                     PWVLRQCATMDEVRALLAKMNLVGTPFAEQFPASQLHWIIADENEAITVEAVADGLHI
                     YENPVGVLTNNPPFAQQMFMLNNYIGLSPKQPENSFAKDVPLSTYSRGMGALGLPGDL
                     SSTSRFARVAFTRMNAVSGDSEAESVSQFFHILGSVDQQRGCCEVAEGKYEITIYTSC
                     CNATRGIYYYTTYDNHRIMGVDMHAEDLDGTTLTCYPMIEEGQVSWQNGQY"
     gene            complement(4818..5669)
                     /locus_tag="ACZO9R_01950"
     CDS             complement(4818..5669)
                     /locus_tag="ACZO9R_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HIR0911868.1"
                     /translation="MEKIRIITDSASDLSASTREGLTILPLAIRFGDTEYLDGVTISH
                     HEFYEKLIESDTLPTTSLVSPAAFEEVYARAVADGETVIAITISGRLSGTYQSATIAA
                     EDFAGKVFVIDSYNATIGEGILVRYALLLKDQGLSAKEIVGILEASKEQIHTLGVLDT
                     LEYLKKGGRISAATAFLGSAMAIKPVVAVSEGEIVMLGKARGSKNGNNFLMKEIEKAD
                     GIDFTRPFCLGYTGLSDEMLQKYIKDSEALWSGHTDHLPVCTLGATIGTHVGPGAIAV
                     AFFSPQS"
BASE COUNT         1522 a         1226 c         1705 g         1263 t
ORIGIN      
        1 tccttgagga aatctcaaag atgaaagaga aaaacctagc agtggaatta ttgaagaagc
       61 taatctctga acaggtgcag atttatcgtc ataccaatat tgtaaaatca cagaaattca
      121 gtgagatcat tcaacgtgca atgaatgctt atctgaacgg tatgttgacg aatgagcaag
      181 tgattgaaga acttctaaat ctggcaaagc agattgcggc tgcaaacaaa gagggagaac
      241 agctggggct tacagcggat gaactggcat tctatgatgc acttacaaaa ccgcaggcaa
      301 ttaaagattt ttatgaaaat gcagagctta ttgcgatcac aaaagaattg gcagatacac
      361 ttcgtaagaa ccggacaatc gattggcaga agcgtgattc agcaagagca aagatgcgta
      421 tcatgataaa gagattgctc aagaagcatc gttatccacc ggaaggaatg gatgatgcag
      481 tgcagacagt tatgatgcag tgcgagctgt ggacggataa caatgatatg gaatcagaac
      541 agaaaaatat aaacgtatat acttattcgt ccaaaccgga attaagcaga gtggcggaaa
      601 aacctgcgcc ttacggaagc agataaaatg aaataggact gatttattgt aaaggcgata
      661 tgacaacatg caaaatcagg acgttgtaaa atgtgcggca atcgattaca attttatctg
      721 ggtcatctcg ggatggcata cacgaaagaa acaggatatt ttccggaaaa acgggaatgt
      781 tctgataagg agagaaacat ggcggttgga ttatgtcaca gcgacaaaaa gtcagtctgc
      841 agaaatctgc agtacaatga gaatgtaaat atgccggaca gccccggatg gaaatgggtg
      901 cagacggtac agacggaagc ggcggaccgt tatctgatcc ggctcaaaag cagggagaaa
      961 ggaagagcgc gcaagacgta ccgcgtggaa gtgaaggaag atgcggtgaa tgcgaaaagt
     1021 gcgcccaatg tggagcagat tgtgcttctg ctgctgttgc ttccactgct ggggattatt
     1081 ttcgcgggac tccgctacag cttaaccgcg gaaaatccgc aggacgatct gcagctggtg
     1141 ctgatactta ccgtgatcgc cgggatactt gggcttagaa tctggctgat acagtggaag
     1201 gcggataaaa cgctccgcta ttttcttatg acgcagatca tcaacgcgcc ggggaacagg
     1261 aacggggcat atgcgggtgc cgcacagcgc accgcagacc agacctgggg ccggacggca
     1321 tatgcacaag atgcagcgta tgcagatgac gcagcatacg cagacgaagg tatcgccggt
     1381 ttgtcggaga tggaagaacc ggaagagcgg gtctgggtgg cggaatggca cctgtcggag
     1441 gcgtaccggc gttacagccg cctgcaggca attccgcttg cggcacttgc attgggggga
     1501 atcatatttt tgtacgcgat tgcgtaccgg tttaatacgt cccatgacac tgcgcgcacg
     1561 atgcttctgc tgggagaggc ggggctttat atgacattgc tcgccctttt gagcaaggtg
     1621 atggagtact gctatctgaa gaaagcaaaa gcgcaggcaa agcgttatca tgccagaatt
     1681 gcccgcgtca taacaaccgg gaaaaacggc gcgaacagct attacctgta tgagtttgtc
     1741 gatgaactgg ggcgaaagtg cgaggtgcgt gccatgtatc cggtgcggcc ggcgaatgcc
     1801 gcaaacgcgc aggatatggc gggcggagag gcggatatct ggtttgacgt agaacacttt
     1861 ccgtatgcat tttcagagaa agacggcgca ccggagatcc gcctgaaact gcgcagtatg
     1921 attgcaggaa cggcagtggc cgcggtcgtg atcgctctgg tgttcgcgta tgggggaacc
     1981 cattacgtgg cgtacaatca ggatgccgac agccttttga tgtcctatga ttccaaaaag
     2041 gcagagtcgg atctgacatt cgaggatgtg cgggacaatc cgaccgtgcg atggatcata
     2101 gacgcctacg cgatctacgt tccgtttgag agcggggaat atggagaact gggcggtcac
     2161 tcactaaatg actgggatca ggagcaggtg aaggaatatc tgtctgactg gtggggcatt
     2221 acgagccggg caacggcgac ccgcacgatc agccagatgc tgaaaaaagg aaccagagcg
     2281 tcctaccgtc atgcgtttga aacttacctg aaaaaagggt atctgtcgat ggatgagaat
     2341 ggttatgtcg atattataag catcagtgag atcccggagg atgagcagtg ccggacatgg
     2401 gtatgctatg atgcgtatgg acatctggac acgcgcggtg tggatgcctg ggattatgtc
     2461 cgtattatgc gcatcacggg actgtgctat cagtgtggct acatcagcct cgaggagtgc
     2521 ctcgaccagt gtctgccgat tgcgcagaga ctgcagaagg aatatggaag ctttgaggag
     2581 atttttgaaa gctacatcta tggatatcaa ttctggaaaa atgacagcga tgacgacagg
     2641 atctattttt accggagggc ggcgggagaa gccgtcgaaa atattcagtc ggagtacaat
     2701 acagaactgg taaaagactg ggagtaggag agaaggatgg aacaggagac acagacaccg
     2761 cacaagcggc gggtgcgtta caagggaaaa tatccgaaga agtttgagga aaaatacaag
     2821 gaactgcagc cggagaagta tcaggacacg atcgagcatg tgatccggaa gggaaatacg
     2881 ccggcgggaa tgcatatctc gattatggta aaagaaattc tggacttttt gcagattgtg
     2941 cctggagagc agggattcga tgcgacgctc ggctacggcg gacacacgcg cgctatgttg
     3001 gaatgtctga aaggacaggg acatatctgg gcgaccgatg tggacccgga ggagtccgcc
     3061 aagacgaaaa agcgtctgga ggatctggga ttcggggagg atcttttgac ggtgcgcctt
     3121 cagaatttct gcacgatcga tgagatcgca aaagaggtcg gcggcttcga ctttattctg
     3181 gcggatcttg gcgtgtcgtc catgcagatc gacaatccga agcgcggctt ctcgtttaag
     3241 gtggatggac cgctggatct caggctcaat caggagacgg ggatcagcgc ggcggagcgg
     3301 ctggatacga tcacgcggga ggaacttgcc gggatgctct atgagaactc cgacgagccg
     3361 tactgtgagg agctggcaaa agcgatcacg gacgagatcc gcaggggcag ccgcatcgac
     3421 acgacgacaa agctgcgcag ggtgatcgag gatacgctgg attttctgcc ggagaaggag
     3481 aaaaaggaca caatcaaaaa gacctgccaa cgcacattcc aggcgctgcg catcgatgtg
     3541 aaccaggagt ttgaggtgct gtatgaattc atggaaaagc ttccgggagc attaagaccg
     3601 ggcgggcgcg tggcaattct caccttccac tcgggagagg acaagcttgt gaagcaggcg
     3661 ctcaaagagg gatataagag tggtatttac agtgactatg cgaaggacgt catccgtccg
     3721 tcggcgcagg agtgcgctgc aaacggaagg gcgcgttcca ccaaaatgcg ctgggcagtc
     3781 agggcgtgaa aaaagagaat atatcacaag ttttattttt aagagggaga agcgattatg
     3841 tgtacagcag caacctatca gacgaaggat ttttattttg gaagaacact ggattatgaa
     3901 ttttcctacg gtgaggagat cgtcattaca ccgcggaatt atgaatttaa cttccggcat
     3961 atgggacagg cagaggagca ttatgcgatc atcggcatgg cgcatgtggc aggggattat
     4021 ccgttgtatt acgatgccat caacgagaag gggatcggca tggcgggtct gaacttcgtc
     4081 ggaaatgccg tctacgcaga ggtgacggag ggaaaagaga atgtggcttc atttgagttc
     4141 attccgtggg tgctccgcca gtgcgcgacg atggatgagg tgcgggcgct tcttgcaaaa
     4201 atgaatctgg tcggaacgcc gttcgccgag cagttcccgg cgtcacagct tcattggatc
     4261 atcgcagacg aaaatgaagc gattacggtg gaagcggtgg cggatggtct gcatatctac
     4321 gagaatccgg tgggcgttct tacgaacaat ccaccatttg cacagcagat gtttatgctc
     4381 aacaactata tcggtctctc cccgaagcag ccggagaatt cttttgcaaa agatgttccg
     4441 cttagcacct acagccgcgg aatgggtgcg ctgggacttc cgggagatct ttcttccact
     4501 tcccggtttg cgcgcgtggc gttcacgagg atgaatgccg tttccggcga ttcggaggcg
     4561 gagagtgtca gtcagttttt ccatatttta ggatcggtgg atcagcagag aggctgctgc
     4621 gaagtggcgg aaggaaaata cgagattacg atctatacgt cctgctgtaa tgcgaccaga
     4681 ggaatctact attacacgac ctatgacaat caccgcatca tgggtgtgga catgcacgcg
     4741 gaagatctcg acggaacaac gctcacctgc tatccgatga tcgaggaagg tcaggtgagc
     4801 tggcagaacg gacagtatta agactgcggt gagaaaaatg cgacggcgat ggcgccggga
     4861 ccgacatggg ttccgatcgt cgcaccgagg gtgcagaccg ggagatgatc tgtatgtccg
     4921 ctccagagag cttcgctgtc tttgatatat ttttgcagca tctcatcgct gagtccggtg
     4981 tagccgaggc agaaggggcg ggtaaaatcg attccgtctg ccttttctat ttccttcatg
     5041 agaaaattat tcccgttttt ggagccgcgt gccttgccga gcatgacgat ttcgccttcc
     5101 gatacggcga cgaccggttt gatcgccatg gcgctgccga gaaatgcggt ggcggcggaa
     5161 atgcggccgc cttttttcag atattccagt gtatccagga caccgagagt gtggatctgc
     5221 tccttggagg cttctaagat tccgacaatt tctttggcgg ataatccctg atcttttaat
     5281 aaaagtgcat atctgaccag aatgccctcg ccgatggtgg cgttatagct gtcgatgaca
     5341 aatacttttc ctgcaaaatc ttctgccgcg atcgtggcac tctggtaagt gccggagaga
     5401 cggccagaga tcgtgattgc aatgacggtc tccccgtccg ccaccgccct ggcatatacc
     5461 tcttcaaaag cggcggggga taccagactg gtcgtgggca gagtgtcgct ctcaatcagc
     5521 ttttcgtaga attcgtgatg gctgattgtg acgccgtcga ggtattccgt atcgccgaag
     5581 cggatcgcaa gcggcagaat ggtgaggcct tctctggtag aagcagagag atcggatgct
     5641 gagtctgtga taattctgat tttttccata agattctcca tatattttta tttttgctga
     5701 ctgtgttcgg tcagat
//
LOCUS       DBMDQJ010000057         5640 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5709_length_5640_cov_1.602094, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000057 DBMDQJ010000000
VERSION     DBMDQJ010000057.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5640)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5709_length_5640_cov_1.602094"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="ACZO9R_01955"
     CDS             complement(<1..801)
                     /locus_tag="ACZO9R_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0911869.1"
                     /translation="MQKVREGRGTDAACMLDYCTQIEEYAERAGDARLLGFSHYYKGR
                     TYYLSNETGKVFEEIGEALGYLEQSGQWELVAAAYNLMAIVSVGKGNLSFAIEYYLAG
                     LKYCKKYELIEVESRIESNLGSLYMECGQYSDARDYLEQAHLDYKRVPEEERSLASLT
                     AIYNNLALCYMKSGSLEKAEEYIRKLETECEAHFEEMDYIYVNCMRARYCHLCGNISM
                     RDQYIADIQNQLSGDVLILDLFDDLYDFCELMLEIDRGDVCRNIGEKLK"
     gene            complement(896..2092)
                     /locus_tag="ACZO9R_01960"
     CDS             complement(896..2092)
                     /locus_tag="ACZO9R_01960"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR0911870.1"
                     /translation="MKFDFTTIMDRKGKDALAVDKIPYKDVQVQEGFSVLPMWVADMN
                     FATVPSVQKAMTDRIAHPAFGYFEPREEYFDAIIRWHAKRNGVQGLTRECIGYENGVL
                     GGVLSALNVFCSRGDKVLVHSPTYIGFTFSLKNNGYEIVHSPLVLDENGVWRMDYEDM
                     EKKICENHIHAAIFCSPHNPCGRVWERWEIERAMEIYEKYQVEVVADEIWSDIILGDH
                     QFVPTQSVSEYARMHTVAMYAPSKTFNLAGLVGSYHIIYNPVIRDRVKKESSLSHYNE
                     MNVLSMYALIGAYQQEGYEWTDELCKVLTKNAEYAYDYIRSHFKGVQVAMPQGTYMLL
                     LDCTEWCRKHGITIDELQKRGASIGVIWQDGRPFHSPCGIRMNLALPFSLVEEAMERL
                     RKYVFV"
     gene            complement(2089..4620)
                     /locus_tag="ACZO9R_01965"
     CDS             complement(2089..4620)
                     /locus_tag="ACZO9R_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR0911871.1"
                     /translation="MKKRGPLLAAFCIPLLITLIICVNREIYPFGDQCMLHIDMYHQY
                     CPFFTELMEKLKTGGSPFYSWNIGLGADFVSLYAYYLASPLNWLLILWPRGYVIEFMT
                     VLSILKIALSGLTFTYYLGEHFHVWQDAGNAAAVTRTEKKTAVRLPADVASYAAALCG
                     AAYALCAFMAAYAWNLMWTDCMVLAPLVILGLERLIRDNRPGLYYVSLAVCILSNYYI
                     SIMICIFLVLWFLLYWMEHRASGYRAWIRFAWYSLLAGATGAVLILPTAKVLSLSGAS
                     GISFPETMEWYFNIVSELARSLLAVDVYTGDSHWPNLYCGIFALFLLFLYVWNRAVPW
                     KKKLPRLALVVFFWLSFSNNMLDFIWHGLHFPTSLPGRQSFLYAFLVLVIAYEALLYI
                     RELKLWQVFAAGGMSVVFLLFCNHFMDETTMEQTSIWASGAFFACYFVIVLGILIGKK
                     RIRQLMLATGCLAVVAELVINYNLTGLDTISRTDYVKNLADYRAVLSETAEKSDEDSV
                     FYRTEELERKTKNDAALSGYHSGTQFSSLMNLNVSHFYQDVGMEGGKNFYCAGGATPL
                     LSAMLSIRYVLADNAMEEGPLRTLVAQRGDTYLYENAYVLPLGFMMDEDVAEKWDYAG
                     GGDIGTQNQLANLLGSDRLLLTAVESESKAGESSFVAQDSAYYYATYSKTGVDNLQEQ
                     VSSGRTRGFTKVSHGYILDLGYCEAGEEVKVTNTANELVQLVVYRLDLMAFRTAYEAL
                     AAQTVEVTEVSDTRVKGNVDVTQAGRLIFSIADEDGWTLYVDGKKRDSETFGSAFIST
                     YLTEGTHEFELRYKSPGFLTGAVISAAAVVLFAATMAVRRKRKKG"
     gene            complement(4617..>5640)
                     /locus_tag="ACZO9R_01970"
     CDS             complement(4617..>5640)
                     /locus_tag="ACZO9R_01970"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HIR0911872.1"
                     /translation="DFKTNEDYLPFLHSLQQGAENTVTGMLNVSVCGGNTADTEYEFL
                     TGNTMAFLPQGSIPYQQYVNGERPSLASYLQSLGYETVATHPYYATGWNRDKVYPWLG
                     FEQSIFKDQYYGARFVRDYVSDASCADKIIQLYEQKEEGRPLFVFNVTMQNHGGYDQT
                     YTNFSPGISVDGVNSISVSQYFSLIKLSDQALEQLIDYFSGVDEKTVIVFFGDHQPSD
                     TVAAPILAMNGMQWNALDEEQQKLRYQVPYVIWANYDIDEEQNADTSANYLGAEVLKR
                     AGVPTDAYQNFLLTLKESYPVISAVRTVEADGSETPAKEEYGEMDIYRKLQYYFMFDW
                     EEESGE"
BASE COUNT         1385 a         1689 c         1175 g         1391 t
ORIGIN      
        1 cttcagtttt tctccgatat tgcggcagac atcaccgcgg tcaatctcta acatcagttc
       61 acagaaatca tacagatcat caaacagatc cagaatcagt acatccccag aaagctgatt
      121 ctgaatatcc gcaatatact ggtctctcat ggaaatattg ccacacagat gacagtaacg
      181 tgcacgcata cagtttacat aaatgtaatc catctcctca aaatgcgctt cgcactctgt
      241 ctccagtttg cggatatatt cctctgcctt ctccaggctg ccgcttttca tataacacag
      301 ggccagattg ttgtaaatcg cggtaaggct tgccaggctg cgctcctctt ccggtacgcg
      361 cttgtaatcg agatgtgcct gctccagata atctctggca tcactgtact gtccgcactc
      421 catatacagg gaacccaggt tactctcaat ccggctttcc acctcaatca gctcatattt
      481 tttgcaatat tttaatcctg ccagatagta ctcgatcgca aacgacagat tgcccttgcc
      541 gacggataca atcgccataa gattgtaggc ggctgccacc agttcccact gcccggactg
      601 ttccagatat ccgagtgcct cgccgatctc ctcaaacacc tttccggtct cattgctaag
      661 atagtaggtt ctgcccttgt aataatgcga aaatccaagc agcctggcat cccccgcacg
      721 ttctgcgtat tcctcaatct gcgtgcagta gtcaagcatg cacgccgcat ccgtgccgcg
      781 gccctctctc actttctgca tcagatcttc catccggtca tcggtcttat gattttccct
      841 agcaagactc ctttgctttt atctgttcgt tcgcgctgca atattttctt aacttctaca
      901 caaatacata cttccggaga cgctccatcg cttcctccac aagtgaaaat ggcagcgcca
      961 gattcatgcg gatgccgcac gggctgtgga acggtctgcc atcctgccag atcacaccga
     1021 tgctggcgcc ccgcttctgc agctcatcga tcgttatgcc atgctttctg caccactctg
     1081 tgcagtctaa gagcagcatg taggttccct gtggcatagc tacctgtacg cccttaaaat
     1141 gggatctgat ataatcatac gcatattctg cattcttcgt caatacctta cataattcat
     1201 ctgtccactc atagccttct tgctgatacg caccgatgag tgcatacatg gaaagcacgt
     1261 tcatttcatt gtaatgtgac agcgatgatt cctttttcac gcggtcacgg ataacgggat
     1321 tgtaaataat atgataactt cccaccagac ccgcaagatt aaaggttttt gacggtgcgt
     1381 acattgccac cgtgtgcatt ctggcatact cggaaacgct ctgcgtcggc acgaattggt
     1441 gatccccgag gatgatatcc gaccagatct catccgcaac cacctcgacc tgatacttct
     1501 catagatttc catggcacgc tcgatctccc agcgctccca caccctgccg cacggattgt
     1561 gcggggagca gaaaattgcc gcatgaatgt ggttctcgca gatttttttc tccatgtctt
     1621 cataatccat gcgccacaca ccattctcat ccagcacaag cggactgtgc acgatctcat
     1681 acccgttgtt tttcaggctg aaggtaaatc cgatgtaggt cggcgaatgc acgagcacct
     1741 tatctccccg cgaacagaac acattgagtg cgcttaagac accgccgagc accccgtttt
     1801 cgtagccgat gcattctctc gtcagcccct gtacgccatt ccttttcgca tgccagcgga
     1861 ttattgcatc gaaatattcc tctcttggct caaaataccc aaatgccgga tgtgcgatac
     1921 gatctgtcat cgccttctgt accgacggca ccgtggcaaa attcatatcc gccacccaca
     1981 tcggaagcac cgaaaatcct tcctgcacct gcacgtcctt gtagggaatc ttatctactg
     2041 ccagtgcgtc ttttcctttt cgatccataa tcgttgtaaa atcaaatttc atcctttttt
     2101 cctctttctt ctcactgcca tggtcgccgc aaagagcacc acggctgccg ccgatattac
     2161 ggctcccgtc agaaatccgg ggcttttgta ccgcagttca aactcatgtg tgccttccgt
     2221 cagataggtg ctgataaaag cgctgccaaa cgtctcactg tcccttttct ttccgtccac
     2281 atacagcgtc cagccatcct catccgcgat ggaaaaaatc agtctgcccg cctgtgtgac
     2341 gtccacgttt ccctttaccc tggtatcgga aacttccgtc acttccaccg tctgtgcggc
     2401 aagcgcctca taggccgtac gaaacgccat gagatccagc cggtaaacca caagctgcac
     2461 cagctcattg gcggtgttag taacttttac ctcttctccc gcctcacaat atcccaaatc
     2521 cagaatatat ccgtgggaaa cttttgtaaa gcctctggtt cttcccgagc ttacctgctc
     2581 ctgcagattg tcaacgcccg tcttgctgta ggtggcataa taatatgcgg aatcctgcgc
     2641 gacaaaagag ctctctcccg cctttgattc ggattccacc gcggtaagaa gcagtctgtc
     2701 gcttcccaga agattggcga gctgattctg tgtgccgatg tcgcctccgc cggcatagtc
     2761 ccatttttcc gccacatctt catccatcat gaatcccagc ggcagcacat acgcattctc
     2821 atacagatac gtgtctccac gctgcgccac aagtgtgcgg agcggtcctt cctccatcgc
     2881 attgtccgca agcacatagc ggatggaaag cattgccgaa agaagcggtg tcgcaccgcc
     2941 ggcacaataa aagttcttgc cgccctccat tccgacatcc tgataaaaat ggctgacatt
     3001 cagattcatc agggatgaga actgtgtgcc ggagtggtag ccggacagcg ccgcatcatt
     3061 tttggtcttg cgctccagtt cctccgtccg gtaaaacacg gaatcttcat cacttttttc
     3121 cgccgtctcc gaaagaaccg cccggtaatc cgccagattt ttcacataat ccgtcctgct
     3181 tatcgtgtca agccctgtca ggttatagtt gatcacaagc tccgccacaa ccgcaaggca
     3241 cccggtcgcc agcatcagct gccggattct cttttttcct atgagtatgc cgagtacaat
     3301 cacaaaatag caggcaaaga atgccccgct tgcccatatg gaggtctgct ccatcgtcgt
     3361 ttcgtccata aaatgattgc agaaaagcag gaatacaacg ctcattccac ccgctgcaaa
     3421 cacctgccac agctttaatt cacggatgta aagcagcgcc tcataagcaa tcacaagtac
     3481 cagaaacgcg tacaaaaaag actgtcttcc cggaagcgat gtcgggaaat gaagaccatg
     3541 ccagataaaa tctagcatat tatttgaaaa gctaagccag aaaaacacga cgagtgccag
     3601 tcttggcagt tttttcttcc atggaaccgc tctgttccac acatacagaa acagcagaaa
     3661 gagcgcaaaa attccgcagt acagattggg ccagtgggaa tctcccgtgt atacatcgac
     3721 tgccagaagg gaccgcgcca gctccgatac gatattaaaa taccactcca tcgtctccgg
     3781 gaacgatatt ccggatgcac cgcttaagga gagcaccttt gccgtcggaa ggatcagcac
     3841 tgcgcctgtg gctcccgcaa gcagcgaata ccacgcaaag cggatccatg cccggtaccc
     3901 cgatgcccgg tgttccatcc agtagagcaa aaaccacagc accagaaaaa tgcagatcat
     3961 gattgaaata tagtaattgc tcagaatgca gaccgccagc gacacataat aaagccccgg
     4021 tctgttatcc cggatcagcc gctctaatcc cagaatcaca agcggcgcaa gcaccatgca
     4081 gtccgtccac atcagattcc aggcatatgc cgccataaac gcgcacagcg catacgccgc
     4141 accgcacaga gctgccgcat aggatgccac atctgccgga aggcgcactg ccgtcttctt
     4201 ctctgtccgt gtgactgccg ccgcatttcc ggcatcctgc cacacgtgaa aatgctcccc
     4261 caggtaatag gtaaacgtaa gaccgctcag tgcaatctta aggatggaca gaaccgtcat
     4321 aaattcaatc acatatcccc tcggccacag aatcaaaagc cagtttaacg ggctggcaag
     4381 gtagtacgca tacagggata caaaatccgc tcccagcccg atgttccacg aatagaacgg
     4441 actgcctccc gttttgagtt tttccatcag ctctgtaaaa aacggacaat actgatggta
     4501 catatcaata tgaagcatac actgatcccc gaacggatag atttcccggt tcacacagat
     4561 gatcagcgtg atcagaagcg gtatgcaaaa cgctgccagt aacggtcctc tctttttcat
     4621 tctccagact cctcttccca gtcaaacatg aaataatact gaagtttgcg gtaaatatcc
     4681 atctcgccgt actcctcctt cgccggagtc tcacttccgt ccgcttccac ggtacgcacc
     4741 gccgatatca ccggatacga ttcctttaat gtcagcagaa aattctgata ggcatccgtg
     4801 ggtaccccgg cgcgctttag cacctctgct cccagataat ttgcgctggt gtccgcattc
     4861 tgttcctcat caatatcgta attcgcccag atgacatacg gcacctggta gcgcagcttt
     4921 tgctgttcct catccagcgc attccactgc atgccgttca tggcaagaat cggcgccgcc
     4981 accgtatcgc tcggctgatg atccccgaaa aagacgatga ccgtcttctc atccacaccg
     5041 gagaaataat cgatcagctg ctctaatgcc tgatccgaca gctttatcag cgaaaaatat
     5101 tggctgactg atatactgtt cacaccgtcc accgaaatac ccggtgagaa gttcgtgtag
     5161 gtctggtcgt atcccccgtg gttctgcatc gtcacattga agacaaacag cggtctgccc
     5221 tcctccttct gctcatacag ctggatgatt ttgtccgcac aacttgcatc gctcacataa
     5281 tctctgacaa aacgtgcccc gtaatactga tctttaaaaa tactctgctc aaatcccagc
     5341 cacggataga ccttgtcacg gttccagccg gtcgcataat agggatgggt cgccaccgtc
     5401 tcatacccga gcgactgcag ataggatgcc aatgacgggc gctctccgtt cacatactgc
     5461 tgatacggaa tacttccctg cggcaggaac gccatcgtat tcccggtcag aaactcatac
     5521 tccgtatctg cggtatttcc accgcacacc gagacattca acatgccggt cacggtattt
     5581 tccgccccct gctgcaggct gtgcagaaac ggcagatagt cctcattggt cttaaaatcc
//
LOCUS       DBMDQJ010000058         5629 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5737_length_5629_cov_1.777315, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000058 DBMDQJ010000000
VERSION     DBMDQJ010000058.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5737_length_5629_cov_1.777315"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..171)
                     /locus_tag="ACZO9R_01975"
     CDS             complement(<1..171)
                     /locus_tag="ACZO9R_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR0911873.1"
                     /translation="MEALMKKREKIMEYVFLLAAVISIVAVALICIFLFANGIPAMKK
                     IGFVEFLTGTKWK"
     gene            complement(271..1239)
                     /locus_tag="ACZO9R_01980"
     CDS             complement(271..1239)
                     /locus_tag="ACZO9R_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0911874.1"
                     /translation="MKKKVMCMLLTGVMAASMFAGCGSSETADTTTDAAATTDAAATA
                     DAADTTSEAAPAENADFDHTSAIAVYSREDGSGTRGAFIELFGVEEKDESGEKVDNTT
                     EEAIITNSTDVMLTSVAGDTYAIGYVSLGSLNDTVKAVKIDGAEATVENIKSGTYKIA
                     RPFNIATKGEVSEAAQDFINYIMSGDGQKVISDNGYIGDDSAAAFESNGAEGKVVVGG
                     SSSVSPVMEKLIEAYKAVNANVEIELQTSDSTTGMTGAADGTLDIGMASRELKDSETE
                     EGLTATKIAMDGIAVIVNQENPVEDLSSDTVKGIFTGGTTTWDAAN"
     gene            complement(1376..1981)
                     /locus_tag="ACZO9R_01985"
     CDS             complement(1376..1981)
                     /locus_tag="ACZO9R_01985"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR0911875.1"
                     /translation="MSEKLLQPMVQLSYAAAMDLVQARLRLINADLTEQCHRQVIRNM
                     SCRIKTPDSVTKKLKKKGREVSFEEAVQTLNDIAGIRVVCFFCDDIYRIADVIKKQKD
                     FHIIKEKDYVKNPKKSGYQSIHVIAGVPVTYNDTVTEIRVEIQIRSFAMDYWAELDTQ
                     MCYKKDAGQIANVERETRGYSDVIAKVDNKMLELRRQIEMM"
     gene            complement(2058..3347)
                     /locus_tag="ACZO9R_01990"
     CDS             complement(2058..3347)
                     /locus_tag="ACZO9R_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0911876.1"
                     /translation="MKTKMSRLEKFWVLYDVGNSAFTLLVSTIIPIYYKNMASANGIS
                     ASDSTAYLSYAISISTLIVAVLGPVLGAVADNKNRKKPLFTFFMMMGVLGCAALAIPR
                     TWILFLAIFVIAKVGFSGSLIFYDSMLVDVTTDERMDEVSSQGYAWGYIGSCVPFVAS
                     LGLILGADAIGISGTVAMMIAFFINAAWWVVVTLPLLRNYQQKYYVEAGGTDVRWIFS
                     RLGKVFAEIRDDKKVLWFLAAFFFYIDGVYTIIEMATSYGKDVGISDTSLLMALLLTQ
                     VVAFPCAILFGKLTQRFAASKLISVCIGAYFVVAVYALWLDAAWKFWMMATFVGVFQG
                     AIQALSRSYYAKIIPKEKSTEYFGIFDIFGKGASFMGTMLMGITTQLFDTSRAGVAAI
                     AAMFVVGFICFQMQRRCMEGETAQSGAMGGAAGFAKE"
     gene            complement(3301..4341)
                     /locus_tag="ACZO9R_01995"
     CDS             complement(3301..4341)
                     /locus_tag="ACZO9R_01995"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0911877.1"
                     /translation="MMPEDRSCAKEEKTAYAVWMAETVEPYLKEHGEKGYLKMEDGMS
                     IAYRRYFLPDAGKCVVISHGFCEFAEKYNEVAYRFLQAGYSVYVPEHRGHGYSGREVD
                     DPELVHVQSYDCYAADLARFVETVVSPGEEHRIVFAHSMGGAIAILALERYPQLFEAA
                     VLSAPMCAMQTGKYPRFLAKLLAEFCCLTGKGKCFATLAGQQGFSETPQFEGSSCLSK
                     ERYDYMFQKRLLDEHYRTYGGSYAWVRAGLRASRKLMRRENLAKIKVPVLLFAAGRDH
                     MVDNDAIARFAEETERTKLFFMPDSRHEIFNADERTRAKYYDEIFRFIKEEEVKDYEN
                     ENEQTRKILGTV"
     gene            complement(4338..5138)
                     /locus_tag="ACZO9R_02000"
     CDS             complement(4338..5138)
                     /locus_tag="ACZO9R_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR0911878.1"
                     /translation="MEKQTLVWAHRGASGYAPENTLEAFRKAVEMGADGVELDVQLTK
                     DGELVVIHDETVDRTSDGSGWVKDFTYARISRFNYNRTHKEGYERAMIPTLEEVYELL
                     KPTGLTINVELKTGVVFYPEIEERVLDLTARMGLEERVWYSSFNHYTVQRIKELNPQA
                     KTGMLYCDGIVNPVSYGALVVGADAMHPALYNLQYPNYMEDCKKHGKRVHVWTVNEEQ
                     YMRMVCEMQVDAMITNYPDVGRRIAAEYLDGTLTPELVRVIKKQGKQG"
     gene            complement(5281..>5629)
                     /locus_tag="ACZO9R_02005"
     CDS             complement(5281..>5629)
                     /locus_tag="ACZO9R_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR0911879.1"
                     /translation="SAGSDGPCTDPDPLLWMYKACNHSNPAESISVQQALRMCTYNGY
                     YTTFDERERGSLEAGKIADMVLLDRNPYDIPKEELASVKVKQLYLRGQEYKKGNGGGA
                     AQILRGMIKRGKC"
BASE COUNT         1234 a         1651 c         1254 g         1490 t
ORIGIN      
        1 cttccacttg gttccggtca ggaattccac aaatccgatt ttcttcattg ccgggatccc
       61 gtttgcaaat aagaatatgc agatcagcgc caccgccacg atggagatga ccgcagccag
      121 cagaaataca tattccatga tcttctctct ttttttcatc agagcctcca tttacaggtt
      181 ttcctaaata acgtgtatgt tccacgaaag gaccggaaga acattctgtt cccccggtcc
      241 gtcctgctat gctattttca gctgtccgtc ctagtttgct gcatcccagg ttgttgttcc
      301 gcccgtgaag atacccttta cagtgtcaga ggagagatcc tctaccgggt tctcctggtt
      361 tacgatcact gcgataccgt ccatggcgat cttcgttgcg gtaagtcctt cctctgtctc
      421 ggaatccttt aactcgcggg aagccatacc gatgtcaagt gtgccgtctg ctgcgccggt
      481 catacctgtg gtagaatcac ttgtctgaag ctcgatctcc acgtttgcat tgactgcctt
      541 gtaagcctcg atcagtttct ccattaccgg agatacggaa gaggaaccgc cgactacgac
      601 tttgccttct gctccattgg actcgaatgc tgctgcggaa tcatctccga tataaccgtt
      661 gtcggagatt actttctgtc cgtcaccgct catgatgtag ttgatgaaat cctgtgcagc
      721 ctcgctgacc tcgccctttg ttgcaatgtt gaacggtctt gcgatcttgt aggtgccgct
      781 cttgatgttc tctacggtag cctctgctcc gtcgatcttc actgccttta ccgtgtcgtt
      841 taaggaaccg agagatacat aaccgattgc gtaggtatca cctgctaccg atgtgagcat
      901 aacgtccgtg ctgttggtga tgatcgcttc ctctgtggta ttatccactt tctcaccgct
      961 ctcatctttt tcttccacac cgaacagctc aatgaatgct cctcttgtgc cggaaccatc
     1021 ctcacgggaa tatacggcga ttgctgatgt atgatcaaaa tctgcattct ctgccggtgc
     1081 agcttcgctt gtggtatctg ctgcgtctgc ggtagctgct gcatctgtgg ttgctgctgc
     1141 gtctgtggtg gtgtctgctg tctcactgct gccgcatcct gcgaacatgc tcgctgccat
     1201 aacacctgtc aataacatac acattacttt cttcttcatt ttgaatcctc cattcaattt
     1261 tacggaatct cttctccgtt ttttctttta cctcgtttct tagaacatgg tttactttac
     1321 gggaggaatg taaaatgcag aagagcactt atgtaaaacc tgtgtaaaaa tcctgtcaca
     1381 tcatttcaat ctgccggcgc agctctaaca tcttattgtc cacctttgcg atcacgtccg
     1441 agtagccgcg cgtctcgcgc tccacattgg cgatctggcc ggcatccttc ttataacaca
     1501 tctgcgtgtc cagctctgcc cagtaatcca tcgcaaacga gcggatctga atctccaccc
     1561 ggatctccgt gaccgtgtca ttgtacgtca ccggaacacc cgcaatcaca tgaatactct
     1621 gatagccgct cttcttggga tttttcacat aatccttttc tttgataata tgaaaatcct
     1681 tctgcttctt gataacatcc gcaatccggt agatgtcgtc gcagaaaaag cacacaaccc
     1741 ggatgccggc gatgtcattg agagtctgca ccgcctcctc aaaagagact tcccgccctt
     1801 tctttttgag ttttttggtg acgctgtccg gcgtcttgat gcggcaggac atattgcgaa
     1861 tgacctggcg gtggcattgt tccgtcaggt cagcattgat cagtcttaat cgtgcctgta
     1921 cgagatccat ggcagcggca taggatagct gcaccatcgg ctgtaacagt ttctcactca
     1981 acaggtcctc attcggcaat ttttgttctc cgttttgttt ttcctttatt tctattcgct
     2041 tctttttctt tttagaatca ttctttggca aaacccgctg cgcctcccat cgcaccgctc
     2101 tgtgccgtct ccccctccat gcaccggcgc tgcatctgaa agcagataaa tcccacaaca
     2161 aacatagcgg cgatcgctgc cacaccggct cttgatgtat caaacagctg cgtggtgatt
     2221 cccatcagca ttgttcccat aaaggaagct ccttttccaa aaatatcgaa gatgccgaaa
     2281 tactccgtcg acttttcctt cgggatgatc ttggcatagt aggaacggga gagcgcctgg
     2341 atggctccct gaaatacgcc gacaaacgtt gccatcatcc agaatttcca cgccgcatcc
     2401 agccagagtg catagaccgc aaccacaaag taggcaccga tgcagaccga aatgagtttt
     2461 gaagctgcaa agcgctgggt cagttttccg aacagaatcg cgcacgggaa tgcgacgacc
     2521 tgcgtgagca gcaatgccat cagaaggctg gtgtcgctga ttccgacgtc ttttccgtag
     2581 gaagtcgcca tctcgatgat ggtatacacg ccatcaatat agaagaaaaa cgctgccaga
     2641 aaccacagca cctttttgtc atcccggatc tccgcaaaga ctttgccgag tctggaaaaa
     2701 atccagcgca catcggtgcc gcccgcttcc acatagtatt tctgctgata gttccgcagc
     2761 agcgggagtg tcacaaccac ccaccatgcc gcgttgataa aaaatgcaat catcatcgca
     2821 accgtgccgg aaatgccgat tgcgtccgcg ccgaggatca gaccgaggct cgccacaaac
     2881 ggcacgcagc ttccgatgta tccccacgca tagccctggg aggacacctc atccatccgc
     2941 tcatccgtcg tgacatccac aagcatggaa tcgtagaaaa tgaggcttcc ggagaatccg
     3001 acttttgcaa ttacgaaaat ggcaaggaac agaatccagg tccttggaat cgcaagcgcc
     3061 gcacagccga gcactcccat catcatgaaa aatgtaaaca gcggcttctt gcggttctta
     3121 ttgtcggcga ccgcaccaag caccggtcct aaaaccgcca caatcagcgt ggagatcgag
     3181 atggcgtagc ttagatatgc ggtggagtcc gatgcgctga tgccgtttgc ggacgccatg
     3241 tttttgtagt agatcggtat aatcgtcgac accagcagtg tgaacgccga atttccgacg
     3301 tcatacagta cccagaattt ttcgagtctg ctcattttcg ttttcataat cttttacctc
     3361 ttcttcctta ataaagcgga atatttcatc ataatatttc gcacgcgtcc gctcatccgc
     3421 attaaaaatc tcatgtctgg aatccggcat aaagaaaagc tttgtccgct ccgtctcctc
     3481 cgcaaaccgc gcgatcgcat cgttatccac catgtggtca cgacctgctg caaaaagcag
     3541 caccggcacc ttgatctttg ccagattctc ccgccgcatc agcttacggc tggcgcgaag
     3601 ccctgcgcgg acccaggcat aacttccgcc gtaggtgcgg taatgttcat ctaagagccg
     3661 cttttgaaac atatagtcat aacgctcttt tgaaaggcag ctgctcccct caaactgcgg
     3721 cgtctctgaa aagccctgct gtcccgccag tgtggcaaag cattttcctt ttccggtcag
     3781 acagcagaac tccgccagca gttttgccag aaaccgcgga tattttccgg tctgcatggc
     3841 acacatcggc gcggagagga ctgccgcctc aaacaactgc ggataacgtt ccagtgcaag
     3901 gattgcaatg gcgcctccca tcgaatgtgc aaacacgatc cggtgttcct ccccgggact
     3961 tacgaccgtc tccacaaacc gcgcgagatc tgcggcatag cagtcataac tttgcacatg
     4021 caccagttcc gggtcatcca cctcgcgccc ggaatacccg tgtccccggt gttccggcac
     4081 atagaccgag tatcccgcct gcagaaaacg gtatgcgact tcgttatatt tttccgcgaa
     4141 ttcgcaaaac ccgtgggaaa tcacaacaca tttccccgca tccggcagaa aatagcgccg
     4201 gtaagcgatc gacatgccat cttccatctt aagatagccc ttctcgccgt gctctttcag
     4261 ataaggctct accgtctccg ccatccagac ggcataagca gtcttctcct ctttcgcaca
     4321 gcttctatcc tccggcatca tccctgcttc ccctgcttct ttatcacgcg caccagctcc
     4381 ggtgtcagcg ttccgtccag atactcggca gcgatccgcc tgcccacgtc cggataattc
     4441 gtaatcatgg cgtccacctg catctcgcac accatgcgca tgtactgctc ctcgttcacc
     4501 gtccagacgt gtacgcgctt tccgtgcttc ttacagtctt ccatgtaatt cggatactgc
     4561 agattgtaga gcgccggatg catggcgtct gcgcccacga caagcgcgcc atagctgacc
     4621 ggattgacaa tgccgtcaca gtagagcatg ccggtcttcg cctgcggatt cagctccttg
     4681 atgcgctgca ccgtgtagtg gttaaacgac gagtaccaca cgcgctcctc cagtcccatg
     4741 cgggcggtca ggtcaagcac gcgctcttcg atctccggat aaaagaccac gccggtcttc
     4801 aattccacat tgatcgtaag tcccgtcggc tttaacagct catagacttc ctcaagcgtc
     4861 gggatcatcg cccgctcgta cccttcctta tgcgtccggt tgtaattaaa tctggaaatt
     4921 ctcgcgtatg taaaatcttt gacccagccg ctgccgtcgc tggtacggtc aaccgtctcg
     4981 tcgtggatga caaccagttc tccatccttt gtgagctgta catccagctc cacgccgtcc
     5041 gcgcccatct caaccgcttt ccggaatgcc tccagtgtat tttcaggcgc gtagccgctt
     5101 gcgccgcggt gcgcccatac caatgtctgt ttttccatgt atacaaaaac ctccaatatg
     5161 atagcttctt atttcttcta tcatatcgga ggtttgtaaa attgttcggg tgcgttgtgt
     5221 aaaatttaga agggatatgt aaaatatctg taagatcacc ctaactggga aaaatgccgg
     5281 tcagcatttt ccccgcttta tcattccgcg cagaatctgt gcggctccgc cgccgtttcc
     5341 ttttttatat tcctgccccc gcaggtagag ctgcttcact ttgacggaag ccagttcctc
     5401 cttcgggatg tcgtaaggat tgcggtccag caacaccatg tccgctattt ttcccgcttc
     5461 cagacttccg cgctccctct catcaaaggt cgtgtaatat ccgttgtagg tacacatgcg
     5521 cagcgcctgc tgcacggaga tggattctgc cggattgctg tgattgcatg ccttatacat
     5581 ccataaaagc gggtccggat ctgtgcaggg tccgtccgat cccgcactt
//
LOCUS       DBMDQJ010000059         5606 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5762_length_5606_cov_1.487193, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000059 DBMDQJ010000000
VERSION     DBMDQJ010000059.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5606)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5606)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5606
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5762_length_5606_cov_1.487193"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="ACZO9R_02010"
     CDS             complement(<1..973)
                     /locus_tag="ACZO9R_02010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0911880.1"
                     /translation="MKYITFTVPCYNSAAYMERCVDSLLIGGEDVEILLIDDGSTDRT
                     GEIADEYERNYPGIVRAVHKSNGGHGSGVNAGLHLATGCYFKVVDSDDWLSEDAYRRL
                     LARVKEFCTGAVAELMDQPDLIVCNYTYNHLEEGTAHTMAYRNVFPAETPCTWNDIGH
                     FRPSQYLIMHALIYRTSVLRETGVCLPEHTFYVDNIFAYQPLPYVKKIYYMDIDLYQY
                     FLGRADQSVNEEVMMRRIDQQIKVTKIVASCVDLDEVRQKYPKLAVYMCRNISIMMAI
                     SSIHLLLINDRAALEKRKLLWNTIREEDKMLYLRLKYTTLSGFTYLPG"
     gene            complement(1289..2008)
                     /locus_tag="ACZO9R_02015"
     CDS             complement(1289..2008)
                     /locus_tag="ACZO9R_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002568732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0911881.1"
                     /translation="MARILLAEDDREIANLERDYLEMSGHEVVIAPDGEEAMKRINEE
                     KFDLLILDIMMPKKSGYDICREIREQIDIPILMVTAKTESVDKIRGLGLGADDYIVKP
                     FDPMELVARVKSQLLRYERLTGKHEMAQEGKEYDADDYCIEVGGLKILTKSWKVIRDG
                     EEIHFPNREFELLKYLAMNPNIVFSKEHLFERIWGYDYMSDSATVTVHINRIREKIEE
                     DPKNPKIIETVWGAGYRLNMK"
     gene            complement(2153..3625)
                     /locus_tag="ACZO9R_02020"
     CDS             complement(2153..3625)
                     /locus_tag="ACZO9R_02020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HIR0911882.1"
                     /translation="MTIRKKLFWTNTFMVLLAMLVLLGIGGSMICLFKDEFLRWYGSH
                     SQLADRYTEVYAGFPEVLESDSWDGMAEQLTDYGFRLIVIDEDRNFVYNNAKHSEEEG
                     TEALFYTPNDAGKITTYLVESTTVLATRVTVDDNFYDIYAVNCPSELSFRGMDRGMFE
                     MFIVVFLVVGILAIGGILLLSQIFSRRLIRHILSPAEALDVAAKRVMDGELTTPIGYP
                     YEDEFKGVCDSFDLMQEHLREEMEKNAAYEKARTEMVSGISHDLRTPLTSIKGYVKGM
                     LDGIANTEEKRTEYLKIAYRKSCDMDVLLSKLFYFSKLETGNMPFFFRECDVERFLRD
                     YVEEKEPEFTERRIAAEVVSDLKAPVYGSLDTEQMIRVFDNLMENACKYANRPDDLRI
                     SIHIAENDGWIVVDFGDNGDGMEQEKLGAVFDEFYRGDESRSSGCDGNGLGLYVCRYI
                     IKEHGGKIRAYTKDGFHVEMCLPEAAEHEKEKAMPEEGRE"
     gene            complement(3622..4245)
                     /locus_tag="ACZO9R_02025"
     CDS             complement(3622..4245)
                     /locus_tag="ACZO9R_02025"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR0911883.1"
                     /translation="MAPKVTRRRRKEENMLEQLMDLDSGFLMYIQEFMRNDAATRTFR
                     GITTLGNAGAVWIAISLLLLVPKKTRRIGCMGLLALVLSLLFNNMLLKHLIARSRPYD
                     CIRGLIPLITPPTDYSFPSGHTAASFAVGVLLFRKLPKRYGIPALVLAALIGFSRLYL
                     GVHYPSDVLAGALLGTGISYAAEVFWLAAEEIVKKHRFCKKRETAHE"
     gene            complement(4331..5194)
                     /locus_tag="ACZO9R_02030"
     CDS             complement(4331..5194)
                     /locus_tag="ACZO9R_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR0911884.1"
                     /translation="MWIFYAFGSALFAGLTSILAKCGIRHTDSDVATAIRTIVVLIFS
                     WIMVFVVGAQDGIAAVSGKTWLFLILSGLATGASWLCYFHALQAGDVNKVVPIDKSST
                     ILTIVLAFLFLGEEVSALKIVCVILIGAGTFLMIQKKETEKSAEQEKKSWLLYACLSA
                     VFASLTSILGKIGIEGLNSNLGTAIRTAVVLLMAWIMVFAKGKQKEIGRIDRRELGFI
                     CLSGLATGGSWLCYYKALQDGLASVVVPIDKLSILVTIAFSYLVFHEKLTKKAAFGLV
                     LIVAGTLLMIL"
     gene            complement(5208..>5606)
                     /locus_tag="ACZO9R_02035"
     CDS             complement(5208..>5606)
                     /locus_tag="ACZO9R_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR0911885.1"
                     /translation="AFSGVWWSMSTLLTVGYGDIYPITTLGRLMAICIAYLGVGVVAI
                     PTGIISAGFVEQYQRKSSISNIRDADIKEIAEIFVDRKYAGKTVGEVQDEQQLTVFLI
                     LRDDLSILPQKDTILKLNDVIVIRERKDVS"
BASE COUNT         1327 a         1708 c         1162 g         1409 t
ORIGIN      
        1 ttcccggaag ataggtaaat ccgctcagcg tcgtgtactt caatctgagg tagagcattt
       61 tgtcctcctc acggatggta ttccacagaa gcttccgctt ctccaacgcg gcgcggtcgt
      121 tgatcagaag gagatggatg gaggaaattg ccatcatgat ggaaatattt ctgcacatat
      181 agacggcaag cttcggatat ttctgccgca cctcgtcgag atcgacgcag gacgccacaa
      241 tcttcgtcac cttaatctgc tggtcaatgc ggcgcatcat cacttcctcg ttcaccgact
      301 gatccgctct tcccagaaaa tactggtaca gatcgatgtc catatagtaa atctttttca
      361 catacggcag cggctgatac gcaaaaatat tatccacata aaacgtgtgc tccggcaggc
      421 agactcccgt ctcgcgcagc accgatgtac ggtagatgag cgcatgcata atcagatact
      481 gggacggtct gaaatgtccg atgtcattcc aggtacaggg cgtttccgcc gggaatacat
      541 tccggtacgc catcgtgtgc gccgttccct cctccaggtg attgtaagta taattgcaca
      601 cgatcagatc cggctgatcc ataagttctg ccaccgctcc cgtgcagaac tccttcacgc
      661 gcgcaagcag tctgcggtac gcatcctccg aaagccagtc atccgaatcc accactttaa
      721 aataacagcc cgtcgcaagg tgcagtcccg cgttgacgcc ggagccgtgt ccgccgttcg
      781 acttgtgtac cgcacgcacg attcccggat agtttcgctc atattcgtcc gcgatctccc
      841 ccgtccggtc ggtcgatccg tcatcaatca gcagaatctc cacatcctcc ccgccgatca
      901 gcagggaatc cacacagcgc tccatatatg cagccgaatt gtaacacggc accgtaaatg
      961 taatatattt cattttgtca aaccgtcctt tctctttcgc ttcatcatct gctctctacg
     1021 atactgcctg cagttaaaca tttcgtgcag aaatttccaa agtttttcca aagaatcctt
     1081 aagtttttct ctgttctctt aacataaggg ataaccttaa acgaagcgca cacaatttct
     1141 aaacaatttc taaacggttc ggtcgatcgg gaaacacaaa atcgggaaac gcaaaaaccc
     1201 cggcggcgat gtacatctct gccatccccg tcggggtctt ttcattattt catttctatc
     1261 attttcgcat ctgcggaaac tttgcgtgct atttcatatt gaggcggtac cctgcacccc
     1321 acacggtctc tatgatcttc gggttcttgg gatcttcctc aatcttctca cggatacggt
     1381 tgatatgcac ggtcaccgtt gcactgtccg acatataatc atacccccag atccgctcaa
     1441 acaggtgctc cttggaaaat acaatgttgg ggttcatcgc cagatacttg agcagttcaa
     1501 attcccggtt cggaaaatgg atctcctcac cgtcacgaat gaccttccag cttttggtca
     1561 gaatcttcag accgccgacc tcaatgcagt agtcatcggc atcgtactct tttccttcct
     1621 gtgccatctc atgcttgccg gtcaaacgct cataccggag cagttggctt tttacgcgcg
     1681 caaccagttc catagggtca aacggcttta caatatagtc gtccgctccc agaccgagcc
     1741 cccggatctt gtccacagat tccgtctttg ccgtcaccat gagaatcgga atgtcaatct
     1801 gctcccggat ctcacggcag atatcatatc cgctcttttt cggcatcatg atgtcaagaa
     1861 tcagaaggtc aaacttctcc tcattgatcc gcttcattgc ctcttctccg tccggtgcga
     1921 tcaccacctc atgcccgctc atctccagat aatcccgctc cagatttgcg atttccctgt
     1981 catcttccgc cagcaaaatg cgtgccatca tcgtttcctc cccgcgtcat ccgccatctg
     2041 tcttctcacg ccctgccaac ttccagcatg gccttttctt ttcactccct gccctcttcc
     2101 ggcatggctt ttcttctcac gccctgccct cttccggcat ggccttttct tttcactccc
     2161 tgccctcttc cggcatggcc ttttccttct cgtgctccgc cgcctccggc agacacatct
     2221 ccacatgaaa tccgtctttc gtgtaggcac ggatctttcc gccgtgctcc tttatgatat
     2281 atctgcatac ataaagtccc agcccgtttc cgtcgcaccc gctgcttctg gactcatccc
     2341 cgcggtaaaa ctcgtcaaag accgcgccca gtttttcctg ctccataccg tcgccattat
     2401 cgccaaaatc cacgacaatc catccgtcat tttccgcaat atgaatggaa atccgcagat
     2461 catcgggccg gttggcatat ttgcaggcat tctccatcag attgtcaaat acccggatca
     2521 tctgctccgt gtcaaggctg ccgtatactg gagcctttaa gtcactgaca acctccgccg
     2581 caatccgccg ctccgtaaat tccggctctt tttcctccac atagtcccgc aagaacctct
     2641 cgacatcaca ctcgcggaag aagaacggca tattcccggt ctcgagcttt gaaaaataaa
     2701 aaagctttga gagcaggaca tccatatcgc aggatttccg gtaggcaatc tttaaatatt
     2761 ccgtcctctt ttcctcggtg ttggcaatcc cgtcaagcat tcccttcacg taacccttga
     2821 ttgacgtgag cggtgtccga aggtcatgtg aaatcccgct taccatttcc gtccttgcct
     2881 tctcgtaggc ggcatttttc tccatctctt ccctcagatg ctcctgcatc aggtcaaaac
     2941 tgtcacacac gcccttaaat tcatcctcgt aaggatagcc gatcggtgtg gtgagttctc
     3001 cgtccatgac tcgctttgcg gcgacgtcga gtgcttccgc cggcgagagg atatgccgga
     3061 taagtctgcg cgagaaaatc tggctgagca gcagaattcc gccgattgcc agaattccca
     3121 ccaccaggaa gacgacaata aacatctcaa acattccgcg gtccatcccc cggaatgaca
     3181 gttcactcgg acagttgacc gcgtagatgt cataaaagtt gtcgtccact gtcaccctgg
     3241 ttgcaagcac ggtcgtgctc tccacaaggt aggttgtaat ctttccggca tcgttcggcg
     3301 tataaaaaag agcctccgtt ccctcctcct cactgtgctt tgcgttattg tagacaaaat
     3361 tcctgtcctc atcgatcaca ataaggcgga agccataatc ggtcagttgt tccgccatgc
     3421 catcccagct gtcactctct aacacctccg ggaacccggc atacacttcc gtgtatctgt
     3481 ccgcaagctg tgagtggctg ccgtaccatc ttagaaattc atccttgaac aggcagatca
     3541 tcgatccgcc gattccaagc agcaccagca tggcaagaag caccataaat gtgtttgtcc
     3601 aaaacagttt tttcctgatc gtcattcgtg tgccgtctcc cttttcttac aaaacctgtg
     3661 ttttttcaca atctcttctg ccgcaagcca gaatacttcc gccgcatagc tgattccggt
     3721 gccaagcagc gctcccgcaa gcacatcact cggataatgc acgcccagat acagtctgga
     3781 aaatccgatc agtgcagcaa gtacgagtgc cggtattcca tagcgcttcg gcagtttccg
     3841 gaacagaagc actcccacgg caaacgatgc cgccgtgtgc cccgacggaa acgagtaatc
     3901 cgtcggaggc gtaatcaacg gaatcagacc gcggatgcag tcataaggtc tgcttctggc
     3961 aatcagatgt ttcaacagca tattgttgaa aagcagcgac agcacaagcg ccagcagccc
     4021 catgcagccg atccgccttg ttttcttcgg taccagaagg agcagtgaaa ttgcaatcca
     4081 gaccgccccc gcatttccca gcgttgtgat tccgcggaaa gtccgggttg ccgcatcatt
     4141 tctcataaac tcctgtatgt acatcaaaaa tccactgtcc agatccatca gctgttctaa
     4201 catgttttcc tcttttctgc gccgtctcgt aactttcggc gccatgcacc tatcccggtg
     4261 cgatacgttt attctaggag ccacgcctaa acagcatctc gaaaaaagtt aaacattttc
     4321 taaacaaccg ttacagaatc atgagaagtg ttcccgccac aatcagcaca agaccaaacg
     4381 ccgccttttt cgtcagcttc tcatggaata ccagatagga aaaagcgatt gtgacaagaa
     4441 tgctcaattt atcaatcggc actaccacgc tcgcaagtcc gtcctgcagc gctttgtaat
     4501 agcagagcca ggaaccgcct gttgcaagtc ccgacagaca gataaatcca agttccctcc
     4561 tgtcaatcct gccaatctcc ttctgcttgc ccttcgcaaa taccatgatc catgccatca
     4621 agagcaccac cgccgtccgg attgccgttc cgagattgga attgagtccc tcgatgccga
     4681 tcttgccgag aatcgaagtg agacttgcaa aaacggctga taggcaggcg tacaaaagcc
     4741 agctcttttt ctcctgctcc gcgctctttt ccgtctcctt cttctggatc ataagaaagg
     4801 taccggcgcc gattaagatg acgcacacga tcttaagcgc gctgacttcc tctcccaaaa
     4861 aaagaaacgc cagcacaatg gtcagaattg tactggactt atcaatcgga accaccttat
     4921 tcacgtcacc tgcctgcaag gcatgaaaat agcacagcca ggatgctccc gtcgcaagcc
     4981 cggagagaat cagaaacagc catgtcttac cggacacagc cgcaatgccg tcctgtgccc
     5041 ccacgacgaa caccataatc cacgaaaaaa tgagaaccac gatcgtgcgg atcgccgtcg
     5101 ccacatccga gtctgtatgg cggatgccgc attttgccag aatcgacgta agtcccgcaa
     5161 acagcgcaga accaaacgca tagaaaatcc acatacgaat gcctcctcta cgatacatct
     5221 tttctctcgc gtatcacgat aacgtcattt aactttaaga tcgtgtcctt ctgcggcagg
     5281 atggataaat cgtcgcgcag gatcaaaaac accgtcagct gctgctcatc ctgcacttct
     5341 cccacggtct ttcccgcata tttcctgtcc acaaaaattt ccgcaatctc cttgatgtcc
     5401 gcatcccgga tatttgagat gctgctcttt ctctgatact gctccacgaa tccggcgctg
     5461 atgataccgg tcgggatcgc taccacgccg acgccgagat acgcaatgca gatcgccatc
     5521 agacgcccca gcgttgtgat cggataaata tcaccgtatc cgaccgtaag aagcgttgac
     5581 atgctccacc agacgccgct gaatgc
//
LOCUS       DBMDQJ010000060         5601 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5765_length_5601_cov_1.534727, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000060 DBMDQJ010000000
VERSION     DBMDQJ010000060.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5601)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5765_length_5601_cov_1.534727"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(137..694)
                     /locus_tag="ACZO9R_02040"
     CDS             complement(137..694)
                     /locus_tag="ACZO9R_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0911886.1"
                     /translation="MEDEKIIALYWDRDQTAIAETQQKYGRYCGSIAHNIVHDAQDAE
                     ECVNDTWMRAWNSMPRERPHLLAAFLGAITRNLSLDCYRRKHSEKRGGGVMPDIYEEL
                     HDCAGGEEPLTQIERKELAASINRFLEGLDRESRIIFMRRYWYMDGIGAIAGRLAVSE
                     SKVKSSLYRSRGKLRMHLEREGLLS"
     gene            complement(711..2063)
                     /locus_tag="ACZO9R_02045"
     CDS             complement(711..2063)
                     /locus_tag="ACZO9R_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012308.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HIR0911887.1"
                     /translation="MERVTAGVLAAVMALGMTGCANSGMSGTTTDLTGQYGTKEEGQT
                     EGTGANGQTGQAEDTGTEDTAMEEKDLGAIREAFEYSYTQFSLNLLRESRKQVQNGED
                     AKNTMVCPLSVMMALEMARTGADGDTKQQMGAVLYPGMSAEDGSMVLGRICKSLPDAE
                     GARFHMSDSVWVKTADDVFVPDENFLDTVKTAYDAEVFGAPFDETTCRDINRLVEQET
                     DGMITEILDQIPELAVMYLVNAVAFDAEWETPYDESQIQDATFYAEDGSGQEVSMMYD
                     TTYRYLTMEHAEGFCRAYKEGYDFVALLPEEGLSLSEWLEELDGETFHETIRQENDTM
                     IETGLPQFTGETDLELKDTLTALGMPLAFDEEQADFSKMGYCPDGRNISISRVLHKTH
                     IDVDGLGTKAGAATVVEMCKEMAMVEQPERIILDRPFLYAIVEKDTGIPVFIGTVESF
                     "
     gene            complement(2206..3879)
                     /locus_tag="ACZO9R_02050"
     CDS             complement(2206..3879)
                     /locus_tag="ACZO9R_02050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR0911888.1"
                     /translation="MEQVYSEEYYDFLIPYGVEAEVPSTPGCVQRIAEDYDIFFYPRE
                     GLPPLSIGNYTYTAIPKCYALLDTTALDASGITRLQNQPVLALKGEGVLVGVIDTGVD
                     YTNPLFRYSDGSSRILRIWDQTVQDGTPPEGILYGAEYRKEEIDEALQAERPYDVVPS
                     RDENGHGTFVTGVVCGGEDIPNGFIGAVPNAGIAVVKLKEAKRYLREFFFVPEGVPAY
                     QETDLMMAVSYLNGVANVLNMPLVICLALGNSMGGHGTDGPLPNFLNYISTRRRRSIV
                     TATGNEANTRHHFQGALRREMDYEDVEISVEQDMAGFFIELWAGAPELYAISVISPTG
                     ERIPKVSLRSGASKSFRFVFEGTTVSVDYRIETKETANQLIYLRFSDVRRGLWIVRVY
                     PENIVSGNYNMWLPMQKLTDGNVIFLRSNPDTTLTAPGTAAQVITVGGYQVSNNSMYA
                     DSGRGYTVAGEIKPDFVAPAVNVYGPGLRGNYVTYTGTSAAAAVTAGAVAQIMQWALV
                     QQNDPVMSNAAIKNMLIRGAKRSEDRGYPNREWGYGALDVYQAFEYLRL"
     gene            complement(3960..5594)
                     /gene="clpX"
                     /locus_tag="ACZO9R_02055"
     CDS             complement(3960..5594)
                     /gene="clpX"
                     /locus_tag="ACZO9R_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR0911889.1"
                     /translation="MNHNDFREIIDDAPETGADTNTGTAGSRQPGSADNGVPAGNAAH
                     GAQTGSEAPRAEDVAEHESTSGTGEVTQSSASDDTDRNQDTEYEDICYICRRPEHIAG
                     KMIRIPNNICICQDCMQKTFDSMNGSGFSMDDMMNMNMGRIPNISMINLSDLQGGLPN
                     NQKLKKKKPKAQSEPVIDIKKIPAPHKIKASLDEYVVGQEQAKKVMSVAVYNHYKRIA
                     ADEKDGVEIEKSNMLMIGPTGCGKTYLVKTLARLLDVPLAITDATSLTEAGYIGDDIE
                     SVVSKLLAAADNDVERAEHGIIFIDEIDKIAKKRNTNQRDVSGESVQQGMLKLLEGAE
                     VEVPVGASSKNAMVPMTMVNTKNILFICGGAFPELEEIIKERLNQEASIGFRAKLKDE
                     YDKDEKLLSKVTVEDVRKFGMIPEFLGRLPILFTLEPLSEDTLVRILQEPKNAILKQY
                     EKLLAMDEVKLAFDEDALRAIAHKAKEKKVGARALRAVIEEFMLDIMYEIPKDDNIGM
                     VTITKDYVEKKGSPVITLRGCIGIGSTENPQIAASN"
BASE COUNT         1101 a         1845 c         1210 g         1445 t
ORIGIN      
        1 cagccatctg cataaagtgt tttttccgtg ctccgagata gcgcacgtcc tcggcttcct
       61 gtatgtatgt cggatccgct tcgccgagcg catccagtaa atcgtccgtc ttcatatttt
      121 ccgctccttt ccgccgctat gaaagcagcc cctcgcgctc cagatgcatc cgcagttttc
      181 cccgggaccg gtacagcgaa gattttacct tactctcgct gaccgcaagc ctcccggcaa
      241 tcgctccgat gccatccata taccagtatc tgcgcatgaa aataatccgg ctctcacggt
      301 ctaacccctc caggaaccgg ttgatggacg ccgccagttc ctttcgctct atctgcgtca
      361 gcggctcctc gcccccggcg cagtcatgca gttcttcata tatatccggc atgacgcctc
      421 cgcctctctt ttcggagtgc tttctgcggt agcagtccag cgacagattg cgcgtgatcg
      481 cacccagaaa agccgccaga agatgcgggc gctccctggg catggaattc cacgcgcgca
      541 tccaggtgtc gttcacgcac tcctctgcat cctgcgcatc atgtacaatg ttgtgcgcga
      601 tgctcccgca gtaacgtccg tacttctgct gtgtctctgc aatcgccgtc tgatccctgt
      661 cccagtacag cgctatgatt ttttcatcct ccatgccttc tccctctcag ttaaaagctc
      721 tccaccgttc cgatgaacac cggaataccg gtgtccttct ccacgatcgc atataaaaac
      781 ggcctgtcta aaataatgcg ctccggctgt tcaaccattg ccatctcctt gcacatctcc
      841 acaaccgtgg ccgctccggc tttcgttcca agcccgtcca catcgatgtg ggtcttgtgt
      901 agcaccctgc tgatgctgat atttcttccg tccgggcagt accccatttt ggaaaaatcc
      961 gcctgttcct catcaaacgc gagcggcatt ccaagcgccg tgagcgtatc ttttagttcc
     1021 agatcggtct cgcctgtaaa ttgcggcaga cccgtctcta tcattgtatc attttcctgc
     1081 cggattgtct cgtgaaacgt ttccccgtcc agttcttcca gccattcgct cagtgacagt
     1141 ccctcctccg gaagcagcgc cacaaagtca tagccctcct tgtaagcccg gcaaaagccc
     1201 tcggcatgtt ccatggtcag atagcggtac gtggtatcgt acatcatgga aacctcctgc
     1261 ccgcttccgt cctccgcata aaatgtggca tcctgtatct ggctctcatc atagggcgtt
     1321 tcccactccg catcaaacgc caccgcattc accaggtaca tgaccgcaag ctccggaatc
     1381 tgatccagaa tctctgttat catgccgtct gtctcctgct ccaccaggcg gttgatgtcc
     1441 cggcaggtcg tctcgtcaaa cggggcgcca aagacttctg cgtcatacgc cgtttttacg
     1501 gtgtcaagga aattctcatc cggcacaaat acatcgtccg ccgtcttcac ccacaccgaa
     1561 tcagacatgt ggaatctggc gccctctgca tccggcaggc tcttgcagat cctgccaagc
     1621 accatactgc catcctccgc cgacattccc gggtacaaaa ccgctcccat ctgctgcttc
     1681 gtgtcgccgt ccgctccggt ccgcgccatc tcgagcgcca tcatcacgga aagaggacac
     1741 accatcgtat tcttcgcgtc ctcgccgttc tgcacctgct ttctgctctc ccgcagcaga
     1801 tttaaggaaa actgcgtata ggaatactca aatgcttccc tgatagcacc gagatctttt
     1861 tcttccattg cagtatcctc cgttcctgta tcttctgcct gtcctgtctg tccgtttgct
     1921 ccggttcctt ccgtctgacc ttcctctttg gtcccgtatt gtccggtaag atcagtggtt
     1981 gtaccggaca ttccgctgtt cgcacagcct gtcatcccca atgccatcac tgccgcaagc
     2041 acacctgccg tcacccgttc cattcctttt ctcctcataa gcttctccct ctcttttcta
     2101 tcatccagtt tttgtctttc atataataag acgcaaaaaa ccggaaacgg ttgcacccat
     2161 ttccggtttt tgtaaaaatt ttctatctca catcttctgt tctctctaca gccttaaata
     2221 ctcaaacgcc tgatagacat ccagcgcacc gtacccccac tcccggttcg ggtaccccct
     2281 gtcctccgag cgctttgcac cgcggatgag catatttttg atcgcggcgt tggacatcac
     2341 cggatcgttc tgctgcacca gcgcccactg catgatctgt gcgaccgccc ccgccgtcac
     2401 cgccgccgcg gcgctggttc ccgtgtaggt cacatagttt ccacgcagcc ccggtccgta
     2461 cacattcacc gcgggtgcca caaaatccgg cttgatctcc cccgccactg tgtagccgcg
     2521 cccggaatcc gcatacatgc tgttattgga tacctggtat ccgccgaccg tgatgacctg
     2581 tgccgccgtc cccggcgcag tgagcgtcgt gtccgggttg gatcttagga aaatcacatt
     2641 tccgtccgta agcttctgca tcgggagcca catattatag ttgccgctca caatattttc
     2701 cgggtaaacc cgtacaatcc acagtcctct tctcacatcg gaaaagcgca gatagattaa
     2761 ctggtttgcc gtctcctttg tctcgatacg gtaatccacc gacacggtcg ttccctcaaa
     2821 gacgaaccgg aatgacttgg aggcaccgga acgcagcgac actttcggaa tccgttcgcc
     2881 cgtcggcgaa atcacagata tcgcgtacaa ttccggcgcg cccgcccaca gctctatgaa
     2941 aaagcccgcc atatcctgtt ccacgctgat ctccacatcc tcatagtcca tctcacgacg
     3001 gagcgccccc tgaaagtggt gcctggtgtt cgcctcgttt cccgttgccg tcacaatcga
     3061 acgcctccgc ctggtactga tatagttcag aaaattaggc agcggaccgt ccgtaccgtg
     3121 tccccccatg ctgttgccga gcgcaagaca gatcacaagc ggcatattca acacatttgc
     3181 aacgccattt aaataggata ccgccatcat caggtccgtc tcctgatagg caggcactcc
     3241 ctccggcacg aagaaaaatt cccgcagata acgctttgcc tcttttagct ttacaacggc
     3301 aatcccagcg tttggcaccg ccccgataaa gccgttcggg atatcctctc ccccgcacac
     3361 gactcccgtc acgaatgttc cgtgcccgtt ttcatcgcgg gacggcacca catcatacgg
     3421 ccgctctgct tgcagcgctt catcaatctc ctctttacgg tactctgcac cgtacagaat
     3481 tccctccggc ggcgttccgt cctgtaccgt ctgatcccag atccgcagga ttctgctgct
     3541 gccgtccgaa taccggaaca gcgggtttgt gtaatctacg ccggtatcga tgacgcccac
     3601 caggacccct tcgcctttta acgcaagcac cggctgattc tgcagccggg tgattcccga
     3661 tgcatcgagc gccgtcgtat cgagcagtgc gtagcatttt ggtatcgccg tgtaggtata
     3721 attcccgata gaaagcggcg gcagtccctc tcgcgggtag aaaaaaatgt cgtaatcctc
     3781 cgcgatgcgc tgcacgcatc cgggcgtgga cggaacctct gcctccaccc cgtacggaat
     3841 caaaaaatca taatactcct ctgaatatac ctgctccatg caatttggca taaaaaactc
     3901 cggtcatcca ttgctttctt aacaatagat gccggagtgt aactatttat tccatattat
     3961 cagttgctgg ctgcaatctg cggattctcc gtactcccga taccgatgca gccgcgcagt
     4021 gtgatgaccg ggctgccctt cttctccaca tagtccttcg tgatcgtgac catgccgatg
     4081 ttgtcgtcct tcggaatctc atacatgata tccagcatga attcctctat caccgcgcgg
     4141 agcgcacgcg ctcccacctt cttctctttc gccttgtgtg cgatcgcgcg cagcgcatcc
     4201 tcatcgaacg ccagcttgac ctcgtccatg gcgagaagct tctcatactg ctttaagatc
     4261 gcattcttcg gctcctgcaa aatccgcacc agcgtatcct ccgagagcgg ctctagtgta
     4321 aacaggatcg gcagacgtcc caaaaactcc gggatcatac cgaacttgcg cacatcctcc
     4381 accgtaacct tggagagcag cttctcgtcc ttgtcgtact catcctttaa cttcgcgcgg
     4441 aatccgatcg aagcctcctg attcagtcgc tccttgatga tctcctccag ctccggaaat
     4501 gctccgccgc agatgaacag gatattcttc gtgttgacca tcgtcatcgg caccatcgcg
     4561 tttttgctgc tggctcccac cggcacctcg acctcggcac cctctaaaag ctttaacatt
     4621 ccctgctgta cggattctcc gctgacatcc cgctggttcg tgttgcgctt cttcgcaatc
     4681 ttatcaatct cgtcaataaa aatgatgccg tgctccgcac gctccacatc attgtccgcc
     4741 gccgcaagca gcttgctgac tacactctca atgtcgtcgc ctatgtaacc tgcctccgtg
     4801 agcgacgtcg cgtccgtgat cgcaagcggc acatccaaaa gccgcgcaag cgtcttaacc
     4861 agatatgtct tgccgcagcc ggtcggtccg atcataagca tgttggactt ctcaatctcc
     4921 acgccgtcct tttcatccgc cgcaatccgc ttgtagtgat tatagaccgc aaccgacatc
     4981 accttttttg cctgctcctg tccgaccaca tattcatcga gcgacgcctt gatcttgtgc
     5041 ggtgccggga tcttcttaat gtcgataacc ggctctgact gcgccttcgg ctttttcttc
     5101 ttcagcttct gattgttcgg cagtccgccc tgcagatccg acagattgat catgctgatg
     5161 ttcggaattc ttcccatgtt catattcatc atatcatcca tggaaaaacc gcttccgttc
     5221 atgctgtcaa acgtcttctg catacagtcc tggcagatgc agatattgtt cggaatacgg
     5281 atcatctttc ccgcaatatg ttccgggcgt ctacagatgt agcagatgtc ctcatactcc
     5341 gtgtcctgat tccggtctgt gtcatccgaa gcggaactct gtgtcacctc tccggtgcct
     5401 gatgtgctct catgctccgc cacatcttct gcacggggag cctcacttcc cgtctgcgca
     5461 ccgtgagccg cgtttcccgc cggcacaccg ttatccgcgc ttcccggctg tctgctgcct
     5521 gcagttcctg tatttgtatc tgcgcccgtc tccggcgcat catctataat ctctctgaaa
     5581 tcattgtgat tcatactaat c
//
LOCUS       DBMDQJ010000061         5579 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5791_length_5579_cov_1.515882, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000061 DBMDQJ010000000
VERSION     DBMDQJ010000061.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5579)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5579)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5579
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5791_length_5579_cov_1.515882"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="ACZO9R_02060"
     CDS             <1..826
                     /locus_tag="ACZO9R_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HIR0911890.1"
                     /translation="DASTFLSVRTPAQWRKITGEAAPDGWTENPLAKRLGLFNDGMLG
                     SVSDYGTYSDNTRQDAGDFSAWTREEELAFQDVLCSSVPSGGEVIVDNPYNDLENAIA
                     DLKTMHVSYLNEDYDRNVLDKWAGTVVNTDDCYDGMDGWSYVERHLGYRLWIAGAALS
                     HSFWADTVEVNAALKNSGFAPFYRQTKVCVSVTDEAGVVCYTDEWEEELLKLSGGNED
                     DRELALTGTIPIRGWESGTYRVYINLSDADSGEQVLLANEADATENGYCLGEVAVR"
     gene            973..1293
                     /locus_tag="ACZO9R_02065"
     CDS             973..1293
                     /locus_tag="ACZO9R_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911891.1"
                     /translation="MQNYEKPEVILMTECSEGVYLASGGQTGEDPTAGGCDSRYMKGA
                     WKKPDYSNSNVQMRAYDRKGCEGCAADDGDGCKLQKGDAKVAGNVYQPVWEQQGLTPD
                     TMVW"
     gene            1425..1760
                     /locus_tag="ACZO9R_02070"
     CDS             1425..1760
                     /locus_tag="ACZO9R_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911892.1"
                     /translation="MKNYERPVMIRTEELAEGVYLASGGIKCDSVYMNGVWQAPDYSD
                     WNGTTRGYKQQFGCLGCPANTASGCGLLSHYVDSGYAGSYDVDNGNRKPSWEKKGYGP
                     DDAVTDWNM"
     gene            1787..2161
                     /locus_tag="ACZO9R_02075"
     CDS             1787..2161
                     /locus_tag="ACZO9R_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911893.1"
                     /translation="MTNYESPVILRTEELAEGVYLASGCYTASAYIHQTPQSGSGEYR
                     IQVNGQHSADHTKEAQTLTISFSLPVTYTSSGGSLVSGSGTNTLVINYNYHQNPTDNI
                     GLGDLVVQADAGLAVTSVNITD"
     gene            2391..4157
                     /locus_tag="ACZO9R_02080"
     CDS             2391..4157
                     /locus_tag="ACZO9R_02080"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911894.1"
                     /translation="MLEKFRHYRERIKEGMLRDMWRQTLWIYEYARLYWKQMIFYTLL
                     GLAGTLVSLISSLISRDMVDIITGQQAGLLARTFCLYIGFSVGNVLISQISNYFANKI
                     SISVDNAIKADIFEKMLLTDLESIQNYHTGDLLTRWSSDASNISSGVLNWIPNLVIYT
                     VRFVSTFAVVFYHDPMFALLALIGMPVSMLMSKTLLRRMKGNNERSAAMSAKMSGFNQ
                     EAFSNIQTIKAFDLIRLYAERLRGLQREYLTMKLEFQKMSMATSILLSIVGLVVSYSC
                     YGFGIYRVWSGAISYGTMTMFLSLSGSLTGTLNQLVSLVPTAVSLTTSAGRLMDILDM
                     PKEDFSDADQVEQFAGQNQSDGIGIVFSGVSYAYHNGKQVFCGADFLARPKEIVALVG
                     PSGEGKTTMLRLMLGLLHPQEGERILIGETDGEKIAVDPSARRLFSYVPQGNTMFSGT
                     IAENMRNVRPEASDEEICEVLKAACAWEFVEKLPDGIYSMVGERGGGFSEGQAQRLSI
                     ARALLRRSPILLLDEATSALDVATERRVLRNIMSTTEPRTCIVTTHRPTVLSICQKVY
                     RIREQKCVQMQEEEIRQMMREF"
     gene            complement(4154..5296)
                     /locus_tag="ACZO9R_02085"
     CDS             complement(4154..5296)
                     /locus_tag="ACZO9R_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR0911895.1"
                     /translation="MKLNDRYIKATLAGIPYLLPYGQLIADPAPATRLNDSGALLWDG
                     ILEGDSREELLALLADRYHATERERAALAEDVDQYLHSLCRMGILLADTPESRGDDTP
                     PLFYRIGPLVFSYRGPSLLYDRFFSAFSCEEEDFDQEVLILTGKPASIPYGAVLIHTE
                     ELTICDSGSSYCFFFAAPWGIREMHVKKDGSRAVLYRMPDPDDMHLEDLFHALRFAFL
                     ILTQQKELYVLHSASFLYRGRAFLVSGSSGTGKSTHSALWHDLYQTPLLNGDLNLLGI
                     RNSIPYAYGLPWCGTSGICTPKDYPLGGIIFLKQAAIDQVQSLQPDEKVLHILQRMIS
                     PAWTKELLLRNLHFSEALAPLIFSCRLYCTKEPSAARTLKAVIDAL"
     gene            5396..>5579
                     /locus_tag="ACZO9R_02090"
     CDS             5396..>5579
                     /locus_tag="ACZO9R_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HIR0911896.1"
                     /translation="MEQIYEIEPLLKAGACIQIHPQGYSMYPFIDPRRDEVVLAGIED
                     GSALRRGDVVLYRRENG"
BASE COUNT         1410 a         1212 c         1734 g         1223 t
ORIGIN      
        1 cgatgcgtcc acgtttctct ccgtgcggac gccggcgcag tggagaaaaa tcacggggga
       61 ggcggcaccg gacggctgga cagagaaccc gctagccaaa aggcttggac tttttaatga
      121 cggcatgctg ggaagcgtga gcgattacgg aacttacagc gataacacca gacaggacgc
      181 cggggatttt tcggcgtgga cgcgggagga agaactggca tttcaggatg tattgtgcag
      241 cagtgtgccg tccggcgggg aagtgattgt cgataacccg tacaatgatc ttgaaaatgc
      301 gatcgcggat ttaaaaacca tgcatgtgag ttacttaaac gaggactatg acaggaatgt
      361 cctggataag tgggcaggga cggtcgtgaa tacagatgac tgctacgacg gaatggacgg
      421 ctggtcttat gtggaacggc atctggggta ccgcctgtgg attgccggtg ccgctttgag
      481 ccattcgttc tgggcggata cggtggaggt aaatgctgct ttaaaaaaca gcgggtttgc
      541 accgttttac cggcagacaa aagtctgtgt gagcgttaca gatgaggcgg gggtagtctg
      601 ctatacggat gaatgggaag aggaactact taagctttcc ggcggaaatg aggacgacag
      661 ggaactggcg ctcaccggaa ccattccgat ccgcggctgg gagagcggga cttaccgggt
      721 atatatcaat cttagcgatg cagacagcgg ggaacaggtt ttacttgcaa acgaagcgga
      781 tgccacagaa aacggatact gcctcggtga ggtggcagtg cgataggtgc acaacgatgc
      841 aagtataaag gcttacaagt gcaaaatcgt acaagaaatc acaagtttgt acgaggaatc
      901 acgccgcaga aaccactata atgaaaagaa aaagaatcct gtatgtggtg ggattgtgga
      961 aggtggtttc ttatgcagaa ctatgaaaaa ccggaagtga ttttgatgac ggaatgttcg
     1021 gagggcgtct atctggcaag cggcggacag accggggaag atccgacggc aggtggatgt
     1081 gacagccggt acatgaaggg agcctggaaa aagccggatt acagcaacag caatgtgcag
     1141 atgcgtgcct atgacagaaa aggctgcgag ggctgtgcgg cggatgacgg tgacggctgc
     1201 aagctgcaaa agggtgatgc aaaagtcgcc ggaaatgtct atcagcctgt ctgggaacag
     1261 cagggactga ccccggatac catggtctgg tagagaaaat gccggcatgc cgttctggca
     1321 gaaaattcat gcttgacagc gcaaacacag aattttatac ttaaaatatg tgtatgagtc
     1381 agcggcagaa gtgccgcaaa aagtgttaga aggtggctgg ttttatgaag aattatgagc
     1441 gtccggtgat gatacggacg gaggagcttg cagagggggt ttatcttgcg agcggaggca
     1501 tcaagtgtga cagtgtctac atgaacggtg tgtggcaggc gccggattat tccgactgga
     1561 atggcaccac acgcggatac aagcagcagt tcggatgtct tggatgcccg gcgaatactg
     1621 cttccggctg cggtctgttg agccattatg tggattccgg gtatgcggga agttacgatg
     1681 tggataatgg taaccgtaag ccgtcatggg agaagaaggg ctatggtccg gatgatgcgg
     1741 tcacagactg gaacatgtaa caatctttaa aacgggggga aatgctatga cgaattatga
     1801 gagtcctgtg attcttagaa cagaagagct tgcggaaggg gtctatcttg caagcggatg
     1861 ctatacggcg agcgcgtaca ttcatcagac accgcagtcc ggaagcgggg agtaccgtat
     1921 ccaggtgaac ggtcagcaca gtgccgatca tacgaaagag gcgcagacat tgaccatttc
     1981 gttcagtctt ccggtaacct acaccagttc cggcggatca ctggtatccg gtagcggaac
     2041 gaatacactt gtgatcaatt ataactatca tcagaatccg accgacaata tcggactcgg
     2101 ggatctggtc gtgcaggcag atgcggggct tgcggtgaca agcgtgaaca ttacagatta
     2161 agaagccggc aggcgaccgg gcggatgtga gaagtcaaaa gcttcgcggg aggaatcggt
     2221 tcctcctgtg aagctctttt ttatgtaata ggaaacttct ttcctattac ataaaaaact
     2281 ctccagggat cgcactgcgg cggagacgaa agatgtcgca agagcgaccc gccgcaggcg
     2341 gagaatcctg cagacagaat tcatttttat gaaagaatag gaaaattaca atgcttgaga
     2401 agttcagaca ttacagagag cggatcaaag agggaatgct ccgtgatatg tggagacaga
     2461 cgctatggat ctacgaatat gcgagacttt attggaagca gatgatattt tatacgcttc
     2521 tgggacttgc gggaacgctg gtatcgctca tttcaagtct catttcccgc gatatggtgg
     2581 atattatcac aggacagcag gcggggcttc tggcgcgcac cttctgcctc tatatcgggt
     2641 tctcggttgg aaatgtcctg atttcccaga tctccaatta ctttgcgaac aagatcagca
     2701 tcagcgtgga caatgccatc aaggcggata tttttgaaaa aatgctgttg acggatctgg
     2761 agtcaatcca gaactaccac acgggagatc ttttaacgcg ctggagttcg gatgcctcca
     2821 atatttcaag cggtgttcta aactggattc cgaatctcgt gatctatacg gtgcgttttg
     2881 tcagcacctt tgcggtcgtg ttctaccatg atccgatgtt cgcgttatta gcgctgatcg
     2941 gaatgccggt cagcatgctg atgtcgaaga cgctgcttcg ccgcatgaag gggaataatg
     3001 agcggagtgc ggcgatgagc gcaaagatgt cgggctttaa tcaggaggcg ttttccaata
     3061 tccagacgat caaggcattt gatctgatcc ggctgtacgc ggagcgcctg cgcggtctgc
     3121 agcgggagta tctcaccatg aagctggagt ttcagaaaat gtccatggcg acctcaattc
     3181 tgctgtcgat cgtgggactt gtggtgtcct acagctgcta cgggtttgga atctaccgtg
     3241 tgtggagcgg tgcgatctcc tacggtacca tgaccatgtt tttatcgctg tcgggctctc
     3301 tcacgggcac cttaaaccag ctggtgtctc tggtgcccac ggcagtgtct ttaaccacat
     3361 cggcgggaag actgatggac attctcgata tgccaaagga agatttttcg gatgctgatc
     3421 aggtggagca gttcgccgga cagaatcagt cggatggcat cggaatcgtc ttttccgggg
     3481 tgagttacgc ctatcataat ggaaagcagg tgttttgcgg tgcagacttt ttggcgcgtc
     3541 ccaaggagat tgtcgctctc gtgggacctt ccggggaggg gaagacgaca atgctccgcc
     3601 tgatgctggg gcttttgcat ccgcaggagg gagagcggat cctgatcggg gagacggacg
     3661 gggagaagat cgccgtagat ccctctgcaa gacggctgtt ttcctatgtg ccgcagggga
     3721 ataccatgtt ttcgggaaca atcgcggaaa atatgagaaa tgtaagaccg gaagcctcgg
     3781 atgaggaaat ctgtgaagtg ttaaaggcgg catgtgcctg ggagtttgtg gaaaaacttc
     3841 ccgatgggat ctattccatg gttggagagc gtggaggcgg attctcggag ggacaggcgc
     3901 agcgtctgtc gattgccaga gcactgcttc gccgatcacc gattcttctg ctggatgagg
     3961 cgacctcggc gttagacgtc gcaacagagc gcagagtgct gcggaatatc atgagtacca
     4021 cggagccacg cacctgtatc gtcacaacac accgcccgac ggtattgtcc atctgtcaga
     4081 aggtgtaccg catccgggag cagaagtgtg tgcagatgca ggaggaagag atccgtcaga
     4141 tgatgcggga attctaaaga gcatcgatga cggcttttaa ggtcctggca gcggaaggct
     4201 ctttcgtgca gtacagccgg caggaaaaga tgagcggagc gagggcttcg gaaaaatgca
     4261 gattgcgaag gagaagctcc tttgtccacg cgggggagat catccgctgc aggatgtgga
     4321 gcactttttc gtccggctgc agggactgca cctgatctat ggcagcctgc tttaagaaga
     4381 tgatgccgcc gagcggatag tctttcgggg tgcagatgcc cgatgtgccg caccatggaa
     4441 gtccataggc gtagggtatg ctgtttctga ttccgagcag gtttaagtcg ccgtttaaga
     4501 ggggcgtctg gtacagatca tgccacaggg cagagtgtgt ggacttcccg gttccggaac
     4561 tgccggaaac gagaaaggcg cgcccgcggt acaaaaaaga ggcggagtgc agcacataaa
     4621 gctccttctg ctgggtcagg atgagaaaag cgaagcgaag cgcgtgaaac aggtcttcga
     4681 ggtgcatatc atccggatcc ggcatgcggt aaaggacggc gcgtgagccg tcttttttta
     4741 catgcatttc gcggatgccc cacggcgctg caaaaaagaa acaatacgaa cttccgctgt
     4801 cgcagatggt aagttcctcc gtgtgaataa gcacggcgcc gtaaggaatg gaagccggtt
     4861 tcccggtcag aataagaacc tcctggtcga agtcttcctc ttcgcaggaa aaagcggaga
     4921 agaagcggtc gtataggaga gagggaccgc ggtaggaaaa gaccagagga ccgatgcggt
     4981 agaaaagagg aggcgtatca tcgccccggc tttcgggtgt atccgcaaga agaattccca
     5041 tacggcacag ggaatgaaga tactggtcga catcttccgc aagtgccgcg cgctcgcgct
     5101 ctgtggcatg gtaacggtct gcgagaaggg cgagcagttc ctcgcggcta tcgccctcta
     5161 agatgccgtc ccacagaagt gccccggaat cgttgagccg ggtggcagga gcgggatctg
     5221 cgatgagctg tccgtaagga agcagatagg gaatacctgc gagcgttgcc ttgatgtatc
     5281 gatcgttgag tttcataccg gtgtcactcc tttattagcg gttcataagt atgcttatta
     5341 tagcattcag aggtaaaaaa tgctacaatg aatgaagaga aacgggaggg gaagaatgga
     5401 gcagatctat gagattgagc cgctccttaa ggcgggagca tgcatacaga ttcatccaca
     5461 ggggtacagc atgtatccgt ttatagatcc gcggagggac gaggtggtac ttgccggcat
     5521 agaggatggg agcgcgctgc ggcgcgggga cgttgtgctc taccgtaggg agaatggaa
//
LOCUS       DBMDQJ010000062         5577 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5794_length_5577_cov_1.548028, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000062 DBMDQJ010000000
VERSION     DBMDQJ010000062.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5577)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5577)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5577
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5794_length_5577_cov_1.548028"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     assembly_gap    535..544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(591..2075)
                     /gene="spoIVA"
                     /locus_tag="ACZO9R_02095"
     CDS             complement(591..2075)
                     /gene="spoIVA"
                     /locus_tag="ACZO9R_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HIR0911897.1"
                     /translation="MDISSKKEFDLYKDIQNRTDGEVYLGIVGPVRTGKSTFIKRFMD
                     LMVLPYMEDVHSRQRTIDELPQSAQGKTIMTTEPKFIPKDAAEIALEDDTRIKIRLID
                     CVGFMVDGATGHMEGSVDRMVHTPWFDHEIPFVEAASIGTEKVIRDHATIGIVVTTDG
                     SIGDLPRENYVNAEEQTVQELEEIGKPYVVVLNSTRPYSEETVRIAEGLREKYQTAVL
                     PVNCEQLRKDDVFHILEQILYEFPVVHMEFYIPKWTEMLPPDHPMKAEIIQSARTILG
                     GMRKVKDIYAQDFTPEHYVSRMKLEEVDLASGCAKIRMEVAEKYYYENMSELAGVPIA
                     GEYELIALVKEMSQRKEAYEKVADAMAAVQVKGYGVVGPGLSDIKMEDPVLIKHGNKF
                     GVRIRATSPSIHMIRANIETEIAPIVGSEEQANDLIAYIREGEKSEEGAWETNIFGKS
                     IGELMENGIRGKISMMDDECQMKLQDTMQKIVNDNNGGMVCIIL"
     gene            complement(2209..3147)
                     /locus_tag="ACZO9R_02100"
     CDS             complement(2209..3147)
                     /locus_tag="ACZO9R_02100"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR0911898.1"
                     /translation="MLYFIVNEKSRSGRGAQIWKEVQRELVKRGIHYRHYVTEHEGHA
                     TRLAVEICAKTDDDICLAVVGGDGTANEVINGITHPEKVRFGLIPTGSGNDLARGLKL
                     SGTPSENLARMLSCMQRGREACTVIDLGEVAWDDCEKPRKFAISAGVGLDALVCKKAL
                     TSKLKIFLNKLHLGKLTYLLITVQSLFSMQTTEAAVRLDETPERKLDRIIYIAAMNFK
                     AEGGGVPMAPKADATDGKLSICSVHGIPKWRTFLCLPFLVAAKHTGIRGFDIDDCGTC
                     EVRLKDPMVLHADGEYCGDVTKLQFRCLPGMLRVML"
     gene            complement(3336..4844)
                     /gene="malQ"
                     /locus_tag="ACZO9R_02105"
     CDS             complement(3336..4844)
                     /gene="malQ"
                     /locus_tag="ACZO9R_02105"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR0911899.1"
                     /translation="MRSSGVLMHISSLPSPYGIGTMGKEARKFADFLEKSGQKYWQIL
                     PICPTSYGDSPYQSFSSFAGNPYFIDLEYLCKEKLLKKAECESFPWGKKADKVDYGVM
                     YESRYKLLKIAFERFLRAEPDDFEAFCEKEADWLSDYALFMALKDANDGNAWFSWEKD
                     LKMRKPEALAEARSTYAKDIRFYQMLQYLFFKQWWELKAYVNEKGIEIIGDVPIYVAG
                     DSADVWANPEEFYLDEDLNPIDVAGCPPDAFSEDGQLWGNPLFRWDVMKKNGYTWWTK
                     RIAAMSKLYDIVRIDHFRGFDSYYAIPAKDKTAKNGEWRKGPGMDLFHTLEQKLGRLN
                     IIVEDLGFLTPSVLKLVADSGFPGMKVIQFAFDSREGSNYLPHTYTEHCVVYTGTHDN
                     DTLLGWMKTAPKESVKFAKEYLNLTKEEGYNWGMMRGAWSSVGELAVVPMQDLIGLGS
                     EARMNTPSTLGGNWQWRATSDQITVKLAKRLYNYMEMYGRLWVGEDADRKEQ"
     gene            complement(4855..>5577)
                     /locus_tag="ACZO9R_02110"
     CDS             complement(4855..>5577)
                     /locus_tag="ACZO9R_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0911900.1"
                     /translation="EDADEVEQAILLCRERKPLGLLFLGGNPEYFHREFSGVDVPCVL
                     VTNRANEMHFENLSSVATDDIAAARCAVDQLFVAGHRRIGILGGDFDKSYTSHQRFLG
                     CEQSFSEHGAVLDVEHCYEKARFSFGSACRAMKRLIGKFPDVTAVFAMSDVMAIGAIR
                     ALRDLGYRVPEDVSVIGFDGTSLAEYYNPKLATIKQQHQTLANRSIEILFGQIELKKE
                     PIHEIVPFEFVNGESIRVIDER"
BASE COUNT         1265 a         1674 c         1116 g         1512 t
ORIGIN      
        1 tgatctgttt ctctttgcat cctgactttt gaccgagatt tggcttcaca gtcgatttcc
       61 tgggacaatt tggttttcct tataatcaat tcattttccc tttcgttttc cgggcttgat
      121 ctggctttcc gtacggattt tggtacctgc atcttttcca aacgggtctg tacggcaaaa
      181 atatcctctc cagacccgtt tttccttgtt ttccatcata tttaacatga aaaaccatag
      241 ggaacgggtc tcaggagcac tttttcttct ctgagacccg aatcacaggg aaaaacgggt
      301 ctcacaggca acttttccca ctatagaccc gttggtcata tcaaattcag gatctgagtc
      361 tcataaacat ttaattataa taccgatctt tatcagaagt gtcaatctat ccgtttctac
      421 acccgggccg gtgtctgtcg tacaaatttc ccacctctgt ttttcctgcc tgtgagttcc
      481 ctgtcacagt ttcaaatgct cattgcgctt cctcattact gatattttct gatgnnnnnn
      541 nnnncctgtc acattttcaa atgctttttc tgcttccttt tttccctgcc ctacaaaata
      601 atgcatacca tcccgccgtt gttgtcgttc acaattttct gcatagtatc ctgcagcttc
      661 atctgacact cgtcatccat catcgagatc ttgccgcgga tgccgttctc catcagctca
      721 ccgatggatt ttccgaaaat attggtctcc caggctccct cctcgctctt ttcgccctct
      781 ctgatgtagg cgatcagatc attcgcctgc tcctcgcttc cgacgatcgg cgcgatctcg
      841 gtctcaatat tggcacggat catgtggatc gagggggagg ttgcgcggat gcggacgccg
      901 aatttatttc cgtgcttgat gagcaccgga tcttccattt taatatcgga gagtccgggg
      961 cctaccacgc catagccttt cacctgtacc gccgccatcg cgtccgccac cttttcatat
     1021 gcctcttttc gctgcgacat ctccttcacc agcgcgatca gttcatattc gccggcaatc
     1081 ggcacacccg caagctcgct catattttca tagtaatact tctcggcgac ctccatgcgg
     1141 attttggcgc agccagatgc gagatccact tcctccaatt tcatccgcga gacatagtgc
     1201 tccggcgtaa aatcctgtgc ataaatatct ttcacttttc gcattccacc gaggatcgtt
     1261 ctggcgctct gtatgatttc cgccttcatc ggatgatcgg gcgggagcat ttccgtccat
     1321 ttcggaatat agaattccat gtggacgacc gggaactcat ataaaatctg ctctaatata
     1381 tgaaagacat catctttgcg aagctgctcg caattcaccg gcaacaccgc cgtctggtac
     1441 ttttcccgaa gtccctccgc aatgcggacg gtctcctcgc tgtagggtct cgtcgaattg
     1501 agcaccacaa catagggctt gccaatctcc tccagttcct gcacggtctg ctcttccgca
     1561 ttcacataat tttcccgcgg caaatctccg atacttccgt ctgttgtcac cacaatgccg
     1621 atcgtcgcat gatcccttat gactttttcc gtaccgatgg acgccgcctc cacaaacgga
     1681 atctcatggt cgaaccacgg cgtatgaacc atacggtcca cgcttccttc catgtgtcct
     1741 gtggctccgt ccaccataaa gccgacacag tcaatcagac ggattttaat ccgcgtatca
     1801 tcttcaagcg cgatctctgc cgcgtctttc ggaataaact tcggttccgt cgtcatgatt
     1861 gtttttccct gtgcagactg gggtaattca tcaatcgtgc gctgcctgct atgtacatct
     1921 tccatatagg gaagtaccat aagatccata aaccgtttga taaacgttga ctttccggtc
     1981 cggaccggtc ctacaattcc aagatacacc tccccgtctg tgcggttttg tatatctttg
     2041 tataaatcaa actctttttt gctgctgatg tccataaaaa taccccttcc atacttaata
     2101 aaatgtatga agggggatct gtaattattc ccgcggaaac taaaatatca tcgcgcgcgg
     2161 atgaccttct ggcgcagaca aagccgggga tctgtacact tcgcttttct acagcatcac
     2221 ccgcagcatt cccggcagac agcggaattg cagcttcgtg acatcaccac agtattctcc
     2281 atccgcatgc agcaccatag gatccttcag ccggacctcg catgtgccgc agtcgtctat
     2341 gtcaaatccg cggattcccg tatgctttgc cgccacgagg aacggcaggc ataaaaatgt
     2401 gcgccacttc ggaattccgt gtacactgca gatcgaaagc tttccgtccg tggcatccgc
     2461 cttcggcgcc atgggaacgc cgcctccctc tgccttaaag ttcatcgctg cgatatagat
     2521 gatgcggtct aacttccgct ccggggtctc gtccaggcgc accgcagcct ccgtcgtctg
     2581 catggaaaaa agcgactgca ccgtaatgag cagataggtc agtttgccca gatgcagctt
     2641 atttaaaaat attttcagct tcgatgtcag ggcttttttg cagactaacg cgtcaagtcc
     2701 cacgccggca ctgatggcga actttctcgg cttctcgcag tcatcccatg ccacctctcc
     2761 cagatcgatc acggtgcatg cctctctccc ccgctgcatg caggagagca tgcgcgccag
     2821 attctccgac ggcgtgccgc taagctttaa ccctctcgcc aggtcatttc ccgagccggt
     2881 cggtatcagt ccgaaccgca ctttttccgg atgtgtgatg ccgttgatga cctcatttgc
     2941 cgttccgtcc ccgccgacca ctgcaaggca gatatcatca tccgtcttcg cgcagatctc
     3001 gacagcaaga cgtgtggcat gcccctcatg ctccgtgaca taatgccggt aatggattcc
     3061 tctcttcaca agttctctct gtacctcttt ccagatctgc gcgcccctgc cgcttctgga
     3121 cttctcattc acaataaaat aaagcatctg tttctcccac cgtaacatac attctgctgt
     3181 ttccggcatg tgccggacat acaccgctct gttccacgtc tgtttttcct atataaacac
     3241 aatcgaaaac gcccacccgg cctctgccag atgagcgttt ctcttctgca atgctctttt
     3301 ttatcgtatc acggatacac ggcaattaca actgtttact gctcttttct gtctgcatct
     3361 tcgcccaccc agagacgtcc gtacatttcc atataattat aaagccgttt ggcaagcttg
     3421 acggtaatct gatctgatgt tgcacgccac tgccagttgc cccccagtgt ggacggcgtg
     3481 ttcatgcgtg cctccgaacc aagaccgatc agatcctgca tcggcaccac cgcaagctcc
     3541 ccgaccgaag accatgcacc acgcatcatg ccccagttat agccttcctc tttcgtgagg
     3601 tttaagtatt cctttgcaaa tttgacgctc tccttcggcg cagtcttcat ccagccaagc
     3661 agcgtatcat tgtcatgtgt tccggtgtac accacacagt gctccgtgta ggtatgcggg
     3721 agataattgc ttccctctct cgagtcaaac gcaaactgta tgactttcat tcccggaaat
     3781 ccggaatccg caacaagctt taacacggac ggtgtcaaaa agcccagatc ctccacgata
     3841 atgtttaatc tgccaagctt ctgctccagc gtatgaaaca gatccatgcc cggtcccttg
     3901 cgccactcac cattctttgc cgtcttatcc tttgccggaa tcgcatagta ggaatcaaat
     3961 ccgcgaaaat ggtcaatccg gacaatatcg taaagctttg acattgccgc aatgcgtttc
     4021 gtccaccagg tgtatccgtt tttcttcatt acatcccaac ggaacaacgg attgccccag
     4081 agctgtccgt cctcggaaaa tgcatccggc ggacaccctg ccacatcaat cggatttaaa
     4141 tcctcatcca gatagaactc ttccggattc gcccatacgt cagcagaatc ccctgcgacg
     4201 tagatcggaa catccccgat gatctcaata cctttttcat tcacataggc ttttagctcc
     4261 caccactgct tgaaaaacag atactgtaac atctgataaa agcggatatc cttcgcatag
     4321 gtgcttctcg cctcggcaag tgcctccggt tttctcatct ttaaatcttt ttcccaggaa
     4381 aaccatgcgt ttccgtcgtt ggcatccttt aacgccataa acagcgcata atcagaaagc
     4441 cagtctgcct ctttttcaca gaacgcctca aaatcgtccg gctctgcgcg aaggaaccgc
     4501 tcaaacgcga tctttaacag cttatagcgc gactcgtaca tcacgccgta atccaccttg
     4561 tctgctttct ttccccacgg gaaagactca cactccgcct ttttcaacag tttttcctta
     4621 cacaggtatt ccagatctat aaaatacgga ttccctgcaa agctggaaaa tgactgatac
     4681 ggcgaatcac cgtaacttgt ggggcagatc ggaagaatct gccagtattt ctgtcctgat
     4741 ttttcaagaa agtctgcaaa ctttctggct tcttttccca tcgtaccaat tccatacgga
     4801 gatggcaggg atgatatatg catcaatact ccactacttc tcataacctc gtccttacct
     4861 ttcatctatc actcgaatac tctccccatt tacaaactca aacggaacaa tctcgtgaat
     4921 cggctctttc ttcaactcga tctggccgaa taaaatctcg atgctgcggt ttgcaagcgt
     4981 ctgatgctgc tgcttgatcg tcgccagctt cggattgtaa tactctgcaa gcgatgtacc
     5041 gtcaaaaccg atcacggaaa catcctccgg cacgcggtac cccagatcac gcagtgcgcg
     5101 gatggccccg atcgccatca catccgacat tgcaaacact gcggtcacat ccggaaactt
     5161 cccgatcagg cgcttcatcg cccggcaggc gcttccaaaa gagaatcttg ccttctcata
     5221 gcagtgctcc acatcaagaa ccgccccatg ctccgaaaag ctctgctcac atcccaggaa
     5281 acgctgatga ctcgtataag acttgtcaaa atcaccgccg agaatgccga ttctgcggtg
     5341 tccagccaca aacagctgat ccaccgcaca gcgtgccgct gcaatatcgt ccgtggcaac
     5401 gctggaaaga ttctcaaaat gcatctcatt ggcacggttc gtcaccaaca cacacggcac
     5461 atccacaccg gagaactccc ggtgaaaata ttccggatta ccgccgagga acaaaagccc
     5521 aagcggcttt ctctccctgc aaagaagaat tgcctgctct acctcatctg catcctc
//
LOCUS       DBMDQJ010000063         5526 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5848_length_5526_cov_1.511336, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000063 DBMDQJ010000000
VERSION     DBMDQJ010000063.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5526)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5848_length_5526_cov_1.511336"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="ACZO9R_02115"
     CDS             complement(<1..491)
                     /locus_tag="ACZO9R_02115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0911901.1"
                     /translation="MRRANVWKKAACVALSLLYFLQIKPQVVQAADYWPEGPEVQSPS
                     VILMEMSTGTVLYEKNSDERNYPASITKIMTTLLALENSDLNEVVTFSDDAINNTEGS
                     GIYRDYGEQMTMEQCLYAVMLNSANECAYAVAEHVGGTVEHFVDMMNEKAAELGCTNT
                     HFA"
     gene            712..1350
                     /gene="trmB"
                     /locus_tag="ACZO9R_02120"
     CDS             712..1350
                     /gene="trmB"
                     /locus_tag="ACZO9R_02120"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HIR0911902.1"
                     /translation="MRLRNIPGADAAITESVYCIKSPETHKGHWQEIFPSQQPLHIEI
                     GMGKGRFMMDLAAAHPDINYLGIERYSSVLLRALQKMEENPLPNLFFICMDAADVAEV
                     FDHGEVDRIYLNFSDPWPKDRHAKRRLTSRQFFARYDEVLAPEGRLEFKTDNQDLFTF
                     SLEEIPEAGWHLDASTRDLHHDPVLNEGNIMTEYEEKFSSLGNPICKLIASR"
     gene            complement(1415..3151)
                     /locus_tag="ACZO9R_02125"
     CDS             complement(1415..3151)
                     /locus_tag="ACZO9R_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0911903.1"
                     /translation="MEQKNIEVQRKKGLNLMMKILITAIIPLIILVAFAGLAIRAVGV
                     TVSDKLMQHELKTSVYAVNQTMSILSSGDWNTDGDQLYKGDYNFSEHQDVLDAFKENT
                     GQDVTLSWNTTRMATSIVSQDGSREIGTEIAADVYQAVKEKGSYFVSDTVIDGQKYYG
                     YYEGLYNSDGSMAGLVFTGMTSASVHEIYNDRLKKNIIFMVVLAIFACIFLAIVMMKI
                     VKAMMSMIDHLDDVAEGNLDSKISDRMTSRSDEIGNIARALYSLIGGLAMIVKNIRTS
                     AKSLDDFSARFQNSFTTINESIHNVNTAMEDIANGATNQAGEMQKVNEQISDMNEAIA
                     ETTKNVENLTGSTEEMKEQNHKLDGTIKDLVEISDRTRESVDEVNRQTDRTNQSVMEI
                     GSAIQMITDIASQTNLLSLNASIEAARAGEHGKGFAVVADEIRQLADQSSDSAKKIGD
                     IVEELIKNSNKSVETMNGVLDEINEQNEKLSTTREAFDGLNTEVGNVVVAIDNIRREI
                     ETVNTAKNDVMSSMESLAAIAQENAASTEETSASMTELSGIVTDCNAQTKNLVDIAEN
                     LSDNVNQFRIKE"
     gene            complement(3313..3385)
                     /locus_tag="ACZO9R_02130"
     tRNA            complement(3313..3385)
                     /locus_tag="ACZO9R_02130"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3350..3352),aa:Lys,seq:ctt)
     gene            3539..4724
                     /gene="pepT"
                     /locus_tag="ACZO9R_02135"
                     /pseudo
     CDS             3539..4724
                     /gene="pepT"
                     /locus_tag="ACZO9R_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
     gene            complement(4912..>5526)
                     /locus_tag="ACZO9R_02140"
     CDS             complement(4912..>5526)
                     /locus_tag="ACZO9R_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911904.1"
                     /translation="AGGLDFGGDSLDLGVDGLDLGTENLDLGADNLDAGAGGLDLGSE
                     GLDLGADSLDLGGDSLDLGADSLDLGAENLDLGTENLDLGVDSLDLGADSLDLGAENL
                     DLGTENLDLGVDSLDLGADGSDLGGESLDLGADLKEEAAPENEAPDEPEPVAAPENEI
                     PDEPKVAKPQKEPETKAEEAAPMDFSDPHKLMTPEEIAALIAKM"
BASE COUNT         1307 a         1495 c         1185 g         1539 t
ORIGIN      
        1 ttggcaaaat gcgtgttggt acatcccagc tccgccgctt tttcgttcat catgtccaca
       61 aaatgctcca ccgtaccgcc aacatgctcc gccactgcgt aggcacattc atttgccgaa
      121 ttcagcataa ccgcgtacag gcattgctcc atcgtcatct gctccccgta atcgcggtag
      181 attccggaac cctcagtatt gttgatcgca tcatcagaaa atgtgacgac ctcattcaga
      241 tcggaattct ccagcgcgag aagcgtcgtc atgattttcg tgatacttgc cggataattc
      301 cgctcatcgc tgtttttttc gtacagaacc gtccccgtcg acatctccat cagtatcacg
      361 gacggtgact gtacttccgg tccttccggc cagtaatctg ccgcctgcac cacctgcggc
      421 tttatctgta aaaagtatag taaacttaag gcgacacatg ccgccttttt ccacacattc
      481 gctcttcgca ttgcttataa ctccattcta tccagaaatc ctgtccattt cttccatata
      541 tatccggaac ataactagta tcagtataat cgcagaaaaa aatacatgca acaatttttc
      601 tggcaaaagc tcttttttta tccacagatt tattgtagaa tatcttgtat gtccacaatt
      661 ataggacttc ccgacattta ttaagatttt cttatgagag gcaggaacaa tatgagactt
      721 agaaatatac cgggtgcaga cgccgccatc acagagagcg tctactgcat caaaagcccc
      781 gagacgcaca aaggccattg gcaggagatt tttccgtcac agcagccgct tcacattgag
      841 atcggtatgg gaaagggccg cttcatgatg gatcttgccg cagcgcatcc cgacatcaat
      901 tatctgggaa tcgagcgcta ctccagcgtc ctgctgcgcg ccctgcaaaa aatggaagag
      961 aatccgctcc cgaacctgtt tttcatctgc atggatgccg ccgatgtcgc tgaggtattc
     1021 gatcacggag aggtagaccg gatctactta aacttttccg atccgtggcc gaaggacaga
     1081 cacgccaagc gccgtctcac ctcccgtcag ttcttcgcgc gctacgatga agttctcgca
     1141 ccggagggac gtctggagtt caagaccgac aatcaggatc tttttacctt ttccctggag
     1201 gagatcccgg aagccggctg gcatctggac gcctccacca gagatctgca tcacgatccg
     1261 gtgctcaatg aaggcaatat catgacggag tacgaggaga agttctcctc cctcggcaat
     1321 ccgatctgca agctgatcgc ttccagataa aaaacacccg cagtctggat ttttctcgat
     1381 ccgcctgcgg gtgttttttg tgttctgttt tcttttattc tttgatccgg aactggttga
     1441 cattgtcgct taaattttct gcaatatcca cgaggttttt cgtctgcgca ttgcagtccg
     1501 tgacgatgcc gcttaactcc gtcatggaag ctgaagtctc ctcggtactt gccgcattct
     1561 cctgtgcaat cgctgccaga ctctccatgc tgctcataac atcattcttc gccgtgttga
     1621 ctgtctcgat ctcacgccgg atattgtcaa tcgcaactac cacattgccg acctccgtat
     1681 tcagtccgtc gaatgcctct ctggtggtcg aaagcttctc gttctgctca ttgatctcat
     1741 ccagcacacc gttcatcgtc tcgacgctct tgttagagtt cttaatcagt tcttccacaa
     1801 tatcaccgat cttcttcgcg gagtcactgg actgatccgc aagctgacgg atctcatccg
     1861 ccaccacagc aaatcctttt ccatgctctc ccgcacgcgc agcctcgatg ctcgcattca
     1921 gggacaacag attggtctgg cttgcaatat ctgtgatcat ctggatcgcg ctgccgatct
     1981 ccatgacaga ctggttcgtc ctgtctgtct gacggttgac ctcatccacc gattctctgg
     2041 tgcggtcgct gatctccacc agatctttga tcgtgccgtc gagcttatga ttctgctctt
     2101 tcatctcctc cgtgctgccc gtgagatttt ccacgttctt tgtcgtctcg gcaatggctt
     2161 cgttcatgtc gctgatctgc tcattgacct tctgcatctc gcccgcctga ttcgtcgctc
     2221 cgttcgcaat gtcctccatc gctgtattca cattatgaat ggactcattg atcgtcgtaa
     2281 agctgttctg gaacctggcg ctgaaatcat ccagcgactt tgcggatgtc cggatgtttt
     2341 ttacaatcat ggccagtccg ccaataagtg aatatagcgc acgcgcaata tttccgatct
     2401 cgtccgagcg gctcgtcatg cggtcgctga tcttgctgtc cagatttccc tccgcaacat
     2461 cgtccagatg atcgatcatc gacatcattg ccttcacaat cttcatcatc acaattgcca
     2521 gaaagataca tgcaaagatt gcaagcacta ccataaatat tatatttttt ttcaatcggt
     2581 cattgtagat ttcgtgaacg gaagcagacg tcataccggt aaacaccaga cctgccatgg
     2641 aaccgtcact gttgtacaat ccctcataat atccgtaata tttctgaccg tcaatcacag
     2701 tatcggacac aaaataacta cccttttctt tcacagcctg atacacatct gctgctatct
     2761 ccgtcccgat ctcgcggctg ccgtcctgac tcacaatgct ggtcgccatc cgggtcgtat
     2821 tccagctgag cgtaacatcc tgtccggtat tttccttaaa agcatccagc acatcctgat
     2881 gttccgagaa attgtaatct cccttgtaca gctggtctcc atccgtattc cagtcaccac
     2941 tgctcaaaat ggacattgtc tggttcacgg catagaccga tgtcttcaat tcatgctgca
     3001 tcagtttatc agacacagta acgccaaccg cccggattgc cagccctgca aaagccacaa
     3061 gaatgatcag cggtatgatc gcggtgatca gaatcttcat catcagattc agaccttttt
     3121 tcctttgcac ctcaatattc ttttgttcca tctgactctc tccccttcat atagtatctg
     3181 ctttttatat cgtcaggttt ttccttctgt taaagtagga tacactcttt ttttaaattt
     3241 taataatatg cgttgtaagc acaaaaaaag caccaacccc acagtcaatg ctgtaaaatc
     3301 ggtacctttc aatgcgccac caggggttcg aaccctggac accctgatta agagtcaggt
     3361 gctctgccag ctgagctagt ggcgcttatt ttgtatctcg tgttctgtat cactcgagca
     3421 cgaaaattat tatataacag aaattttaga aatgcaacat ttttttttaa aaaacttcaa
     3481 attttttttc aattgtgtta ttttatagat agaatggcat agatcaagga ggtttattat
     3541 gcaagttgaa cagcgattat tgaaatatgt gtcctattgg acgacatcgg atgagaataa
     3601 catggcagac ggcaagatac agattccgtc taccgagcgt cagtttgatc tcggaaaggt
     3661 actggagcag gaattacggg atctcggtct taaaaatgtg attctgaccg accactgcta
     3721 tgtgtacggt ctgcttccgg caactgccgg ctgtgaggga cacaaggccg ttggttttat
     3781 ctcacatatg gataccgcac cggactactc cggcaaggac gtcaaaccac agatcattcc
     3841 ggattatgac ggtgggaacg tccgtttaaa cggtaccggg gcaatgttaa aggtttccga
     3901 ttttccaacg ctgaaggatt tgaagggaag aaccctgatt accaccgacg gcacgacact
     3961 gctcggcgcg gatgacaagg ccggtgtggc agaaatcgtc acagcgctcg aagagatcat
     4021 ttcttccgga atgcctcacg gtgatatctg ggtgggattt accccggacg aagaaatcgg
     4081 ctgcggtgca gatttatttg acctggatta ttttaaggct gcttttgcct acacggtaga
     4141 cggcgattac gagggcgaag tcgcctatga gaatttcaac gccgcgagcg caaaattttc
     4201 cattaaaggc gtcaatgtgc atccgggcga ggcaaaggat attatggtaa atgccgctct
     4261 tgtggcgtgc gatatccagt catctgaccg acatgaaggg tgatgtcgct tttgcagaac
     4321 tttcctatat cgtgcgtgac cacgacaggg cttcttttga atcacgcctg aacagactcc
     4381 gcacactgga agtggagatc aaccagaaat acggcaccgg aaccgtctct cttaccatca
     4441 cggattccta ctccaatatg cttgagatca tcgaacagca tccttatgtc gtggacattg
     4501 caaaaaaggc aatctcgatg accggtctca caccgctctc acgtccggtc cgcggcggta
     4561 cagacggcgc cagattaagt tttatgggtc ttccgtgccc gaatcttggc accggcggct
     4621 acggcttcca cggtccgtat gagcatatca gcgtggaggg catgcagaca gcagtctcca
     4681 tcatcaagaa tatcgtgacg atcgtcgcgg agtctgatct gtaagattac ggaaagaatt
     4741 gccgcataga ttcccgtaat aggaagttcc gggatatata aataagtatt atcgtttcag
     4801 ataaaaaatg agcgtggaag aaattctcac attttacttc aaagaatctt ccacgctcat
     4861 tttttattct gaaactgcta ataacttcat ttcttatccc ggaacctgct cttacatttt
     4921 tgcaatcaat gcggcaattt cttccggagt cataagcttg tgtggatcgg aaaaatccat
     4981 cggtgcagct tcctctgcct ttgtctcagg ctctttctgc ggcttggcca ccttcggctc
     5041 gtccggtatt tcgttctccg gcgcggctac tggctctggc tcgtccggtg cttcattctc
     5101 cggcgcggct tcctctttca gatctgctcc aagatcgagg ctttctcctc cgagatcaga
     5161 gccatctgct cctaaatcta agctatccac tccgagatca aggttctccg ttccaaggtc
     5221 aaggttctct gctccaagat cgagactatc tgctcctaaa tctaagctat ccactccgag
     5281 atcaaggttc tctgttccaa ggtcaaggtt ctctgctcca agatcgagac tatctgctcc
     5341 taaatcaagg ctgtctcctc cgaggtctaa gctgtctgcc ccaagatcaa gtccttctga
     5401 tccaagatca agtccgcctg ctccggcatc taagttatct gctcctaggt caaggttctc
     5461 tgtcccgaga tcaagaccgt ccactccaag gtctaagctg tctcctccga aatcaagacc
     5521 gcctgc
//
LOCUS       DBMDQJ010000064         5487 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_5892_length_5487_cov_1.593762, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000064 DBMDQJ010000000
VERSION     DBMDQJ010000064.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5892_length_5487_cov_1.593762"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..>663)
                     /locus_tag="ACZO9R_02145"
     CDS             complement(100..>663)
                     /locus_tag="ACZO9R_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR0911905.1"
                     /translation="DWTSFFRLCKTAPKSDVIVLVATFLLTVFFDLVIAIEVGVVLAA
                     MLFMKRMAETADVTAWKYLDEPDVTPGEAEKLRDIPHTIRVFEISGPLFFAAADQILQ
                     INSDKATRVIVIRMRSVPAIDASAMKSLRELADRAKKKKITLVFSHVNEQPMSVMVKD
                     GFTEYVGAQNFHANIVEALDYAENLVK"
     assembly_gap    664..673
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(714..1853)
                     /locus_tag="ACZO9R_02150"
     CDS             complement(714..1853)
                     /locus_tag="ACZO9R_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR0911906.1"
                     /translation="MNELKPKLFDVLKDYSKEQAMRDVISGIIVAIIALPLSIALAIA
                     SGVGPEQGLYTAIIAGFFISFFGGSRVQIGGPTAAFVVIIYGIVTDYGTAGLTVATIL
                     AGIFLIVMGICHFGSLIKYIPYTITTGFTCGIAVTLFVGQLKDFFGLSIASVPSGFVD
                     KVIAYATNISTINWTATAVGAAAIVIMLLWPKVTDKIPGSLIAIIVTTAVVYFADLPV
                     NTIGSVYGELSSSFPTPHVPALSMKLVQEMLSPAFTIALLAGIESLLSAVVSDGMIGD
                     THKSNAELVGQGLGNIFSGIFGGIPATGAIARTAANVRNGGRTPVAGIVHCITLTIIL
                     LVLMPLAALIPMTTLAAVLLIVAANMQRRSSLSFQDVDTLDIVRI"
     gene            2044..2403
                     /locus_tag="ACZO9R_02155"
     CDS             2044..2403
                     /locus_tag="ACZO9R_02155"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HIR0911907.1"
                     /translation="MIINDIGRKAAIMAESGNSYQKIYEIVKQIPKGCVASYGQVAAL
                     AGNPRWARVVGYALHVNPDPAHIPCYRVVNREGRLSDAFAFGGVNEQKLLLEAEGVEV
                     TDGYVDMELYQWKKFRI"
     gene            2422..3057
                     /locus_tag="ACZO9R_02160"
     CDS             2422..3057
                     /locus_tag="ACZO9R_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HIR0911908.1"
                     /translation="MQKKELALQVIERLKKEYPDTGCSLDYDDAWKLLVSVRLAAQCT
                     DARVNVVVQDLYREFPDVDALADADVEEIERIVKPCGLGHSKARDISACMKMLKEKYQ
                     GKVPEDFDALLELPGVGRKSANLVMGDVFGQPAIVTDTHCIRLANRIGLVDGIREPKK
                     VEMALWKLIPPEESNDFCHRLVDHGRAVCTARTAPYCDRCCLNDICKKAGI"
     gene            3186..5183
                     /locus_tag="ACZO9R_02165"
     CDS             3186..5183
                     /locus_tag="ACZO9R_02165"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003505165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR0911909.1"
                     /translation="MNIAAKYGKSYFMPPEVTYDWVKKDSIEKPPIWCSVDLRDGNQA
                     LIEPMGLEEKLEFFRMLVDIGFKEIEVGFPAASDTEYNFMRALIERDMIPKDVTVQVL
                     TQAREHIIRKTFEAVKGAPHAVIHLYNSTSVAQREQVFKKSKEEVRQLAVDGAELLKT
                     LAAETDGNFTFEYSPESFPGTEVDYAVEVCNAVLDVWKPDAEHKAIINIPTTVQIAMP
                     HVFACQIEYIHKHLKYRDNVVLSVHPHNDRGCGISDAEFGILAGADRVEGTLFGNGER
                     TGNVDIVTLAMNMVCHGVDPKLDFSNISEIREKYERFTGMRVYERTPYAGDLVFTAFS
                     GSHQDAISKGMAWREAGKSGDRWDVPYLPLDPKDVGREYESDVIRINSQSGKGGVAFV
                     LKQNFGMDLPDKMKEEVGYLVKGVSDRRHQELSPEAIYRIFEEHYVNLCDVFQISECH
                     FEQKDGITSRLVIEHNKERRTIETTGNGRLDAVSNAIKMYFGISYELAVYEEHAISEG
                     SSSKAAAYVEIICKGKNYWGVGIDEDIITSSIAALVSAANKMLKSENIVEGREERIVD
                     IMNYIQNHYADVTLDVLADQFNLSKPYLSKYIKEKSGMTFQDAVKKARMKKARTMLKE
                     TNQTVESIAAYVGYENVEHFNRLFKKAYDMTPVQYRKQSAE"
BASE COUNT         1538 a         1150 c         1583 g         1206 t
ORIGIN      
        1 aaaaaaggca ccggtgcatc ttgtgcaccg atgccttttt cctgctatta ttgtaacagt
       61 ttggcggatg ttcataaagt agatgtaaga ggaacaggac tattttacca gattttctgc
      121 ataatccaga gcttctacga tatttgcatg gaaattttgg gctccgacgt actcggtaaa
      181 gccatctttg accattacgg acatcggctg ttcgttgacg tgggagaaaa ccagtgtaat
      241 ctttttcttt tttgcacggt cggccagttc gcgcagtgat ttcatggcac tggcgtcgat
      301 ggcagggacg ctgcgcatgc ggatgacaat cacgcgggta gccttgtcac tgttgatctg
      361 taagatctgg tctgcggcgg cgaaaaacag cggaccactg atctcgaaga cacggatggt
      421 gtgaggaata tcgcgcagtt tttccgcttc gcccggggta acgtccggct catccagata
      481 tttccatgcg gtcacatcgg cggtttctgc catacgcttc atgaaaagca tggcggcgag
      541 cacaacgccg acttcaatcg cgatcacaag atcgaaaaat acggtgagca ggaaagttgc
      601 caccagcaca atgacatcgc tttttggcgc tgttttgcag agacggaaaa aggaagtcca
      661 gtcnnnnnnn nnntagtgtt agtggagctg actatgcgaa ccgtgccgta gacttagatc
      721 cgaacgatat cgagagtatc aacatcttga aaggacaagc tgcttctgcg ttgcatgttg
      781 gcagccacaa tcagaagaac agcggcaagc gttgtcatcg ggatcaaagc ggcaagcggc
      841 atgagcacca gaaggataat ggttaaggta atacagtgca cgatgccggc gaccggggtg
      901 cggccgccgt tgcggacgtt ggctgcggta cgggcgatcg caccggttgc cgggataccg
      961 ccgaaaatac cggaaaaaat atttccgagt ccctgaccga ccagctcggc gttggatttg
     1021 tgggtatcac cgatcattcc gtccgagacg acagcggaca gcagggattc gatgcctgcc
     1081 agcagcgcga tggtaaatgc cggtgagagc atttcctgca ccagtttcat ggacagtgcc
     1141 ggaacatgcg gtgtcgggaa ggaggaagaa agctctccgt agacactgcc gattgtgttg
     1201 accgggagat ccgcgaagta gacaacggcg gtcgtgacaa taatcgcaat cagagatccc
     1261 ggaatcttgt cggtgacttt cggccagagc agcatgatga caatagctgc agcgcccaca
     1321 gcggtggcag tccagttgat ggtggaaata tttgtggcgt aggcaatgac cttatccacg
     1381 aacccggacg gcacggatgc gatggagagt ccgaagaaat ccttgagctg gccgacaaac
     1441 agggtcacag cgataccgca ggtaaaaccg gttgtgatgg tataaggaat atatttaatg
     1501 agagaaccaa agtggcagat tcccattacg atcagaaaaa tgccggcaag aatggttgcg
     1561 acagtaaggc ctgccgtgcc ataatccgtg acaatgccat agatgatgac tacaaaggct
     1621 gccgtaggac caccgatctg cacacggctg ccgccgaaaa acgagataaa aaatccagcg
     1681 atgatggcag tatagagccc ctgctccggc ccgacgccgg atgcgatggc aagcgcgatc
     1741 gacagtggca gggcaatgat ggcaacgatg ataccggaga tcacgtcgcg catggcctgt
     1801 tctttgctgt agtcctttaa gacgtcaaat agttttggtt tcaattcatt cattttcatt
     1861 tcctcagtct gtaattatta aatttttata aacacaagtc aaaattttag cactttacaa
     1921 acgcgaacgc aagaacgcac gggaaaaata caagcacatg gaaaggtgag caaaactggg
     1981 aaaaatggaa gacattttac aggaaaaaga agatgtgaaa agccggattg tatgttaaga
     2041 tagatgataa taaatgatat aggaagaaag gcggcaatca tggcggagtc gggcaattct
     2101 tatcagaaga tctatgaaat cgtaaagcag attccaaagg gatgtgtggc atcttacgga
     2161 caggtggcgg cgcttgcagg gaatcccaga tgggcaagag tggttggcta cgcgcttcac
     2221 gtgaacccgg atccagcgca tattccctgt taccgtgtgg tgaaccggga aggcagactc
     2281 tcggatgcct ttgcatttgg cggtgtgaat gagcagaagc tgctgctgga ggcagagggc
     2341 gtcgaagtaa ctgacggcta tgtggatatg gaactgtatc agtggaagaa gttccggatt
     2401 taagagagac ggaggagaaa tatgcaaaaa aaggaactgg cacttcaggt gatagagcgt
     2461 ttgaaaaagg agtatccgga cacgggatgt tcactggatt atgacgatgc atggaagctt
     2521 ctggtcagtg tgagactggc ggcacagtgt acggatgcga gagtcaatgt ggtcgtgcag
     2581 gatctgtacc gggaattccc ggatgtggat gcacttgcgg atgcagatgt ggaggagatt
     2641 gagcgtattg taaaaccgtg cgggctggga catagcaagg cgcgcgacat ctccgcctgc
     2701 atgaagatgc tgaaggaaaa gtaccaggga aaagtaccgg aagattttga tgcgctgtta
     2761 gaacttccgg gagtcggcag aaaaagcgcc aatctggtga tgggggacgt ctttggacag
     2821 ccggcaatcg tcactgatac gcactgtatc cgcctggcga accgcattgg gctcgtggac
     2881 gggatcaggg aaccaaagaa agtggagatg gcactctgga agctgattcc gccggaggag
     2941 agcaatgatt tctgccacag gcttgtggat cacgggcgtg cagtctgtac cgcgcggaca
     3001 gcgccatact gcgaccgctg ctgtctcaat gacatctgca aaaaggccgg aatttagaaa
     3061 aagataaaat aaatcaatat atttgattaa aaacgcacct tgcggaagca taaggggtat
     3121 gatagtatat acgaaatgaa taacagaaat caactttctt gaattatttt gtatggagga
     3181 aagacatgaa tattgcagca aaatacggaa aatcatactt tatgccgccg gaggtgacat
     3241 atgactgggt gaagaaggac agcattgaga agccgcccat ctggtgcagc gtggatctgc
     3301 gggatggtaa tcaggcgctg attgagccga tgggactgga ggaaaagctt gaatttttcc
     3361 ggatgctggt cgacatcgga tttaaggaga tcgaggtcgg ttttccggca gcttcggaca
     3421 cggaatacaa ctttatgcgc gcgttaatcg agcgcgacat gatcccgaag gatgtgacgg
     3481 tgcaggtttt aactcaggcg agagaacata ttataaggaa gacatttgag gcggtcaagg
     3541 gagcgcctca cgccgtcatc catctgtaca attccacatc ggtggcacag cgggagcagg
     3601 tgtttaagaa gagcaaggaa gaagtcaggc agctggcagt ggacggggca gaattgttaa
     3661 agacactcgc ggcggagacg gacggcaatt ttacgtttga gtacagtccg gaaagcttcc
     3721 cgggcacaga ggtggactat gcggttgagg tctgcaatgc agtgctcgat gtctggaagc
     3781 cggatgcaga gcacaaggcg atcatcaaca ttccgaccac ggtacagatt gcgatgccgc
     3841 atgtatttgc ctgccagatc gagtatattc acaagcattt gaaatacagg gataatgtgg
     3901 tgttgagcgt tcacccgcac aatgacagag gatgcggtat cagcgatgcg gagttcggta
     3961 ttcttgccgg tgcggaccgc gtggagggca ctcttttcgg aaacggagag cgcaccggaa
     4021 acgttgatat tgtgacgctg gcgatgaata tggtgtgcca cggtgtcgat ccgaagcttg
     4081 atttttccaa tatcagcgag atccgcgaga aatacgagcg ctttaccggt atgcgggttt
     4141 acgagcgtac accgtatgcc ggagatctgg tatttaccgc attttccggt tcccatcagg
     4201 atgccatctc caagggtatg gcgtggagag aagcgggcaa gagcggcgac cgctgggatg
     4261 tgccgtacct gccgctggat ccgaaggatg tcggaaggga atacgagtcg gatgtcatcc
     4321 gcatcaacag ccagtccggc aagggcggag tcgcattcgt attaaagcag aacttcggta
     4381 tggatctgcc ggataagatg aaggaagaag tcggttatct ggtcaagggt gtatcggatc
     4441 gcagacatca ggaactctcg ccggaagcaa tttaccggat ttttgaagaa cattatgtca
     4501 acctgtgcga tgtttttcag atttcagagt gtcatttcga gcagaaggac ggcatcacat
     4561 cacgcctggt gatcgagcac aacaaggagc gccgcacgat tgagacgaca ggaaacggcc
     4621 gcctggacgc cgtgagcaac gcaatcaaga tgtatttcgg catcagctac gagcttgccg
     4681 tatacgagga gcacgccatt tcagaaggat catcatccaa ggctgcggcg tatgtggaga
     4741 tcatctgcaa gggcaagaat tactggggcg tcggaattga cgaagatatc atcaccagct
     4801 cgatcgcggc attggtatcc gctgccaaca agatgttaaa gagtgagaac attgtggagg
     4861 gacgcgagga gcggatcgtc gacatcatga attacataca gaatcactat gcggatgtga
     4921 cgctggatgt gctggcagat cagtttaatc tctccaaacc gtacctttcc aaatacatta
     4981 aggaaaagtc tgggatgacc ttccaggatg cggtaaagaa agcacgcatg aaaaaagcac
     5041 gcactatgtt aaaggagacg aaccagacgg tagagtcgat tgccgcctac gtgggttacg
     5101 agaatgtgga gcatttcaat cgcctgttca aaaaggctta tgatatgact ccggtacagt
     5161 atcggaaaca gagtgcggaa taacaggagt ctattcagca aattaaattt aggaatggct
     5221 ttttaagtgt tgattatcgg gtaaacaaag tataacataa ttttagcatt gtataaaaat
     5281 atgctgaata ttgacaattt atgacagtaa aattatacta aagtagtatg tggtcataga
     5341 ataagaccac aacttatgga gggacaagat aatgaataac agagagaaag caaaggcagt
     5401 acaaaagaaa ttcaaccagc gactgggaat ttattacggc gtgatggcag caggtgtcat
     5461 tgtggcagcc atcggactga tgacaca
//
LOCUS       DBMDQJ010000065         5374 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6025_length_5374_cov_1.538214, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000065 DBMDQJ010000000
VERSION     DBMDQJ010000065.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5374)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6025_length_5374_cov_1.538214"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..475)
                     /locus_tag="ACZO9R_02170"
     CDS             complement(<1..475)
                     /locus_tag="ACZO9R_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911910.1"
                     /translation="MIKQPVSAGFGFIVPEEELEAHLEDKEGTVYFRQTDIDCETMPK
                     LNTKSYYYEVIYTPYILHGKTCAKDVKIGAGHPYPEKKSTAPVAVTAVTKKIVSKTLP
                     LAEYLEAEETRYAGASPKLGIISFYNGHYALISDCYINKKLVTDQEYAPDGAIAIF"
     gene            complement(602..886)
                     /locus_tag="ACZO9R_02175"
     CDS             complement(602..886)
                     /locus_tag="ACZO9R_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911911.1"
                     /translation="MVQEAVSVTLEKMHPCADYAKVWIDGNGEVNTAEETVLQTGDIA
                     QVRYVGLITAYRTDNGTGYISGEYWFHVNNLTDAGEASQLVLGRRVSFTI"
     gene            complement(862..1512)
                     /locus_tag="ACZO9R_02180"
     CDS             complement(862..1512)
                     /locus_tag="ACZO9R_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911912.1"
                     /translation="MGEDITVRESGIYLLNRVEVTLKDDSRAEGILQNIAAETITVEG
                     REIMLSDVADIRLAGELTDYNVMRDTGMIGNFTFAPEACRDAAELELMRYGDFACTVS
                     CHLKLAAGKICADDIRLVSHTHKVCVEKLSQTEAIYRFFDGSFYFSRMHSGRTALSGT
                     ATSHRIMRRWRTPDCRAEISCFRRIRLTLDSNRAISTWFPTLAPKNLVRWCRKRSV"
     assembly_gap    1910..1919
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2117..3484)
                     /locus_tag="ACZO9R_02185"
     CDS             complement(2117..3484)
                     /locus_tag="ACZO9R_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0911913.1"
                     /translation="MFAKINFHDEFYQKLKLLVLPITMQQFMLALVSATDAIMLGAVS
                     QTSLSAVSLAGQIQFVLNLFISGISAGSGIMAAQYWGKKDAASIEEVMPIALRTNLIF
                     SGAFTVFAAVAPEVLMRIFTSDPALIESGAVYLRAVSLSYLLCGISQIYLILLKNTGY
                     ATVSSRISCTAVVVNIICNAILIYGLLGLPALGIQGAAYATVAARLVELIWSYLETKQ
                     SGRVQIIWKKMFRAADAVLTKDFWRYTTPVLGAALVWGIAYVSYSVIMGHMGSDAVAA
                     NSITTIAKNMLSCLIRGVSGGAGVLIGNLLGAGELDKAKVYGGRLTRMAIVVGMTTGG
                     LLMLISPLIVRFAPLSETAAEYLQYMLIFCGCNIMAQSVNTTVLDGIFCAGGDSKFDM
                     KGNLGAMWCFSVPFGFFAAFVLKWPVMVVYCIVNLDEIVKIPAVYLHYKKYIWVRNIT
                     VQEEA"
     gene            complement(3626..>5374)
                     /locus_tag="ACZO9R_02190"
     CDS             complement(3626..>5374)
                     /locus_tag="ACZO9R_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR0911914.1"
                     /translation="YDGEIYFADGREKVTGHAAAYTRETPFALEPTLTEVRLQDEMPV
                     REATVQGDVTILDFGQNFAGIVEIDPSFLTNETITIRHGEILNADGSLYTANLRKAKA
                     TIVYHAGAEKKKYRPRFTYMGFRYMELSGAAYQPGMIRAYALHTEMERTGYFSCENPL
                     VQKLYENQVWGQKSNYVEVPTDCPQRDERMGYTGDGQVFALTGAYNFNTDAFWKNFLR
                     DLELGQLDNTEGYVCATVPQTGPAGIGFISMLGWGNAVTILPEMMYRQFGDEEALPRQ
                     YESMKLFVEAEIRKMGKKNLWIGPSLGDWLAMGKGIAWQAMHNNPVSNAFIVHDLKVI
                     SETAERLGKREDAARYRRQLAATTDAYIKKFVSKNGIVAKDYQSAYIMALKFVLPEGE
                     LREMVKKNFVANIRKNGLQTGFFATEHLLPLLVEAGEQKLAYDVLLQENCPGWMYQVK
                     CGATTTWERWDALKPDGTVNEEKMAGSGENMVSFNHYAFGSVGEFYYQYILGIRPEKP
                     GFAKLHFAPYPDERLGGVSGSYLSVAGKIESAWRYEPDGCHIRLATPVEAVVLLPDGR
                     SENVPAGTYEWTVKRP"
BASE COUNT         1216 a         1617 c         1153 g         1378 t
ORIGIN      
        1 tgaagatcgc aatcgcgccg tccggcgcat actcctgatc tgtcacaagc tttttattga
       61 tatagcagtc gctgatcagc gcataatgcc cgttgtagaa ggagataatt ccaagcttcg
      121 gagaagcccc cgcatagcgc gtctcctccg cttccagata ttccgcaagc ggcagtgtct
      181 ttgaaacaat cttttttgtc actgcggtca ccgccaccgg agcggtcgac tttttctccg
      241 gatacggatg tccggcaccg atcttcacat ccttcgcgca tgtcttgcca tgcaggatat
      301 acggggtata tataacctcg taataataac ttttggtatt cagcttcggc atcgtctcac
      361 aatcaatgtc tgtctgcctg aaatagacgg ttccctcctt atcctccaga tgggcttcca
      421 gttcttcctc cggtacaata aaaccgaacc ctgcactgac cggctgcttg atcatgtatt
      481 ttacgatata accgcgcagt ctctccggtt tggattcttc ctgcttctcc tctgcaatga
      541 ccgtcttctc tgagatcaga gcgacatcct tcgccgcgca aagcttaccc tttaaggtct
      601 gctagatcgt aaacgaaacc ctgcgcccca gaaccagctg cgacgcctcc ccggcatctg
      661 tgagattgtt gacatggaac cagtattccc cggaaatata accggtaccg ttatccgtgc
      721 ggtaagccgt aatgagtccc acataccgta cctgcgcgat atcgccggtc tgcagcaccg
      781 tctcttctgc ggtatttacc tcgccgttcc cgtcaatcca cactttcgca taatccgcac
      841 aggggtgcat cttctccagc gtcacactga ccgcttcctg caccatctca ccaggttctt
      901 tggcgcaagt gtaggaaacc acgtagatat tgcccggttt gaatccaaag tcaatctgat
      961 cctgcgaaaa catgatattt ccgcgcggca gtccggcgtt ctccatcttc gcataattct
     1021 gtgagacgta gcagttcccg ataaagccgt tcgtccggag tgcattcggg aaaaataaaa
     1081 tgatccatca aaaaaacggt aaattgcttc cgtctggctt aatttttcca cgcatacctt
     1141 gtgtgtgtgc gataccagcc ggatgtcatc cgcgcagatc tttccggctg caagcttcag
     1201 atgacaggac acggtacacg caaaatctcc ataccgcatg agttccagtt ccgcagcatc
     1261 acggcaggct tccggcgcaa atgtaaaatt cccgatcatt ccggtatcac gcatcacatt
     1321 ataatctgtc agttcccctg ccagccggat atccgccaca tccgaaagca tgatctccct
     1381 gccctctaca gtgatcgtct ccgccgcaat attctgcagg attccctctg ccctgctgtc
     1441 atctttcagc gtcacttcga cccggtttaa taaataaatc ccgctctctc tgaccgttat
     1501 atcttcaccc atacaagcta ctccttctgc aatccatcat gtatttatgg tattacagca
     1561 cccgcgcttt tgcaaggaat tctttccacg caacccgccc ggagattcac ggtgctcgtc
     1621 aaatctgcat acagtcatac aatataaata atgtctttct gcatttcata atctcccggc
     1681 ggattcatgc aactttctgt accatggttg tatttattcg ggctgctctg tattctgaaa
     1741 cttcgggtct acagccgata aaatacgccg tgagacccgt tttctggaat attttgatca
     1801 ttttttctat ctgctggagc gttctcgggt ctacagccta caaaatacgc cgtgagaccc
     1861 ggaattttac aatttccggg tctcacggcg tattttttgc ttccagtgcn nnnnnnnnnc
     1921 ttatcaagcg gttttggggt ctcacgcagc tatttttcct ctgtagaccc gcgccagcct
     1981 tctccaggct ttctcacaaa cgtcttctgc aataaaaaat atgccacgac acatttttgc
     2041 accaagcgcg gtcgcctgcg gcatgtctct ccttcgcgca agtgcgcatc ctgcccggag
     2101 ggctaaaagc accttgttac gcttcttcct gtaccgtaat attccgcacc cagatatatt
     2161 ttttataatg taaatacact gcaggaatct tcacaatctc gtcgaggttc acgatacagt
     2221 acaccaccat gaccggccat ttcagcacaa atgccgcaaa gaagccaaac ggtacggaaa
     2281 agcaccacat tgccccaaga tttcccttca tgtcgaactt ggaatcccca ccagcacaga
     2341 agatgccgtc cagcactgtc gtgttgaccg actgcgccat gatattgcag ccgcagaaga
     2401 tcagcatata ctgcagatac tccgccgccg tctcactgag cggtgcaaaa cgcacgatca
     2461 aaggcgagat cagcatcaga agaccgccgg tggtcatgcc gaccacgatt gccatacgcg
     2521 tcagtctgcc gccgtacact tttgccttgt ccagttctcc ggcaccgagc agatttccga
     2581 tcagcacgcc cgcaccgccg ctgacgccgc ggatcaggca cgataacata ttcttggcga
     2641 tcgtggtgat ggaattcgcc gccactgcgt cgcttcccat atgtcccata atcacagagt
     2701 aggacacata ggcgattccc cagacaagcg ccgcgccgag caccggcgtg gtataacgcc
     2761 agaaatcttt tgtcagaacg gcatccgccg cacggaacat tttcttccag atgatctgca
     2821 cacgcccgga ctgcttcgtc tccaggtaac tccagatgag ctccaccaga cgggcggcaa
     2881 ccgtcgcgta agccgctccc tgtattccta acgcaggaag ccccaacagc ccgtagatca
     2941 agatcgcatt gcagataata ttcacaacga ccgctgtaca gctgatccgt gaactgaccg
     3001 tggcatagcc ggtatttttc agcaaaatca ggtaaatctg tgaaataccg cacagcagat
     3061 atgacaggga caccgcgcgc agatagaccg ctccgctctc aattaacgca ggatcggacg
     3121 taaaaatgcg catgagcacc tcgggtgcca ccgccgcaaa cacggtgaac gcaccgctga
     3181 atatgagatt ggtgcgcagt gcaatcggca tgacctcctc aatggaagcc gcgtcctttt
     3241 tcccccaata ctgcgctgcc atgattccgg agccggcgga gataccgctg ataaacagat
     3301 ttaagacaaa ctggatctgc cctgccagag acaccgctga cagagaggtc tggctgaccg
     3361 ccccaagcat aatggcgtcc gtggcgctga ctagcgccag cataaactgc tgcatcgtga
     3421 tcggcagcac cagaagcttt aatttctggt agaattcatc gtggaaattg atttttgcaa
     3481 acataatttt catcctttac atgtataact catgagattt tatcatctat gccttgccgc
     3541 cgtcaatttc ctttcttgcc ttttttcagt cagattccgg atttttttta catatcctgc
     3601 actttttaca tattcgatct tttcactatg gtcttttcac cgtccactcg tacgttccgg
     3661 caggcacatt ctcacttctt ccatccggaa gaagcactac cgcctccacc ggcgttgcaa
     3721 gccggatatg gcagccgtcc ggttcgtacc gccatgcgct ctcgatcttc cctgccacgg
     3781 aaagatagct tccggatacg cctcccaggc gctcgtccgg ataaggtgca aaatgcagtt
     3841 ttgcaaagcc cggtttttcc ggtctgattc ccaggatata ttgatagtaa aactccccca
     3901 cactgccgaa tgcgtaatgg ttaaaggata ccatattttc accggagccc gccattttct
     3961 cctcgttaac agttccgtcc ggctttaagg catcccagcg ctcccaggtt gtcgttgcac
     4021 cgcatttcac ctggtacatc caccccgggc agttctcctg cagtaataca tcataggcaa
     4081 gtttctgctc tcccgcttcc accagaagcg gcagcagatg ctctgtcgca aaaaatcctg
     4141 tctgcagtcc gtttttacga atgttggcga caaagttttt cttgaccatt tcccgcagtt
     4201 cgccctccgg cagcacaaat tttaatgcca tgatgtaagc ggactgatag tccttcgcca
     4261 cgattccgtt tttactcacg aattttttga tataagcgtc cgtcgtcgcg gcaagctgtc
     4321 tgcggtaccg cgccgcatct tctcgctttc caagccgttc tgccgtctcg ctgatgacct
     4381 ttaaatcatg cacgatgaac gcgtttgaca ccggattgtt gtgcatcgcc tgccatgcga
     4441 ttccttttcc catcgcaagc cagtcgccta gggacggacc aatccagaga tttttcttcc
     4501 ccatcttgcg gatctccgcc tccacaaaaa gcttcatgct ctcatactgg cgcggcagtg
     4561 cctcctcgtc cccgaactga cggtacatca tctccggcag aatcgtgacc gcattgcccc
     4621 agcccagcat gctgataaat ccgatcccgg caggtcctgt ctgcggcacc gtggcgcaca
     4681 catatccctc ggtgttgtca agctgcccca gctccagatc ccgtagaaaa tttttccaga
     4741 acgcatccgt attaaaatta taagcccctg tcagtgcaaa cacctgaccg tccccggtat
     4801 agcccatgcg ctcatcccgc tgcgggcagt cggtcggcac ttccacatag ttggatttct
     4861 gtccccagac ctggttctca tagagtttct gcaccagcgg attctcgcag gaaaaatatc
     4921 cggtacgctc catctctgta tgaagtgcgt atgccctgat catccccggc tgatatgccg
     4981 caccggaaag ctccatgtaa cggaatccca tataggtaaa gcgcggacgg tattttttct
     5041 tctccgcgcc tgcatggtag acgatcgttg cttttgcttt gcgcagattt gcggtgtaca
     5101 ggctgccgtc ggcatttaag atctccccgt gccggatcgt aattgtctca tttgtcaaaa
     5161 aagaaggatc tatttctaca attccggcaa agttttgccc gaaatcaagg atcgtcacgt
     5221 ctccctgcac cgttgcctct ctcaccggca tctcatcctg cagacgcacc tccgtaagcg
     5281 tcggctccag tgcaaacggc gtctccctcg tgtaggcagc cgcatgaccg gtcactttct
     5341 ctcttccgtc tgcaaaataa atctcaccat cgta
//
LOCUS       DBMDQJ010000066         5341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6057_length_5341_cov_1.270038, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000066 DBMDQJ010000000
VERSION     DBMDQJ010000066.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6057_length_5341_cov_1.270038"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            217..>710
                     /gene="holA"
                     /locus_tag="ACZO9R_02195"
     CDS             217..>710
                     /gene="holA"
                     /locus_tag="ACZO9R_02195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR0911915.1"
                     /translation="MKTLNEDIKTGNFKPVYLLYGEEAYLKKQYRDRITKAIFPDGDT
                     VNYAYYEGKGINPGELIDLAETMPFFADRRLIVIENSGFFKNASPELADYIKTMPDTA
                     CFLFVENEADKRGKMYKAVKSKGRVVEMARQDEKTLLYWVAGNVKKEGYKIKEQTARY
                     LLSTT"
     assembly_gap    711..720
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <721..1212
                     /gene="holA"
                     /locus_tag="ACZO9R_02200"
     CDS             <721..1212
                     /gene="holA"
                     /locus_tag="ACZO9R_02200"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR0911916.1"
                     /translation="QTKLKIQQSEKELEKLFCYCMGKEEIEVRDIEAICTTQITNKIF
                     EMVEAVAVRNQKKALDYYYDLLALKEPPMRILYLLTRQFKLLLEVKDLAGKRYDKAAI
                     AKTVGLHPFVAGKYMQQCRTFEKKELRSILEDAVDTEELVKTGRLNDVMSVELFIVKY
                     SSV"
     gene            1266..3077
                     /gene="lepA"
                     /locus_tag="ACZO9R_02205"
     CDS             1266..3077
                     /gene="lepA"
                     /locus_tag="ACZO9R_02205"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HIR0911917.1"
                     /translation="MSMDQSKIRNFCIIAYIDHGKSTLADRIIEKTGTLTSREMQAQV
                     LDNMDLERERGITIKAQAVRTVYKASDGEEYIFNLIDTPGHVDFNYEVSRSLAACDGA
                     ILVVDAAQGVEAQTLANVYLALDHDLDVMPVINKVDLPSAQPDEVVKEIEDVIGIEAE
                     DAPRISAKTGLNVDQVLEQIVQKIPAPAGDPKAPLQALIFDALYDSYKGVIVFCRIKE
                     GTVRVGDPILMMATGAQSEVTEVGYFGAGQFIPCDELSAGMVGYICASIKNVRDTRVG
                     DTVTNAAHPCEEPLPGYKKVNPMVYCGMYPADSAKYPDLRDALEKLQINDASLQYEPE
                     TSLALGFGFRCGFLGLLHLEIVQERLEREFHLDLVTTAPGVIYKVHKTNGEVIDLTNP
                     SNLPDPSEIEYMEEPVVSAEIMVTSEYVGAIMTLCQERRGVYNGMEYLEETRALIKYD
                     LPLNEIIYDFFDALKSRSRGYASFDYELKGYERSELVKLDILINKEQVDALSFIVFKD
                     SAYDRGRKMCEKLKEEIPRHLFEIPIQAAVGGKVIARETVKALRKDVLAKCYGGDISR
                     KKKLSEKQKEGKKRMRQVGNVEIPQEAFMSVLKLDED"
     gene            3167..4399
                     /gene="hemW"
                     /locus_tag="ACZO9R_02210"
     CDS             3167..4399
                     /gene="hemW"
                     /locus_tag="ACZO9R_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR0911918.1"
                     /translation="MELYIHIPFCVKKCQYCDFLSFAADEATQAAYVEALLREIAYYG
                     PHCSDYLVSTVYIGGGTPSWLTEEWMAQIMSAVRRSFRLASDAEISIECNPGTVTDHK
                     FAGYRNAGINRISIGLQSAVDEELKILGRIHTFDQFLKTYELARKNGFDNVNVDIMSS
                     LPGQTAESFARTLQQLLWLKPEHISAYSLIIEKGTPFYDLYKFDAVKQQAGMQTVALP
                     TEDEVYRMTKLTEQVLAEAGYVHYEVSNFAKPGYACRHNIGYWERENYLGLGLGASSL
                     MENIRYSNTRELYVYLEETRHIREGIWENEITDSQGSGASGRVEQLPATNLHASAERI
                     TRKGQMEEFMFLGLRMMEGISRERFAQTFGTQIEAVYQEVLLHLTEEGLLEKRAGRIY
                     LTERGQDVSNYALAQFLL"
     gene            4511..>5341
                     /locus_tag="ACZO9R_02215"
     CDS             4511..>5341
                     /locus_tag="ACZO9R_02215"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR0911919.1"
                     /translation="MNVTKRQKRRIRNLVCIQYSIVKMLLVIAGFELLGRGLSLMQGT
                     QSATQEIEGEVIWSEETKTEENPLIVCVDAGHGGKDNGSDYKGRYEKDDNLKLAQAVS
                     AYLADKNVKVVMTRSDDTFLRLSERCGIANQSGADYMLSLHRNDGEGNGVETWISASG
                     SEETDALANHIMEVLDTAGIQRNRGVKKGTQTGESSNYYINVHSDMPSCIVEMGFIND
                     ASDNQLFDEKLDAYAAAIGDAVLATAETYRANKVTDGNGQGAGEAGSGTGDGQGTDGT
                     G"
BASE COUNT         1491 a         1055 c         1601 g         1184 t
ORIGIN      
        1 atatcagcag ggagaaagaa ccgttacgga catccgcatg atctgacgct gaaaagactt
       61 gaaaaatcgg gtgcaaagat atattctaca caggataacg gagcggttgc cgtgacggtc
      121 aggaaaaaaa ctatgtggat tcacggtgga aacagttgaa cttaaagtcg gaattcagta
      181 tacttttaag aggtgaaaga agatgggaga gaaccgatga aaactttaaa tgaagacata
      241 aaaacaggaa attttaagcc ggtatatctc ctgtacggag aagaggctta cctgaaaaag
      301 caatacagag accggatcac gaaagcaata tttccggacg gggatacggt gaattatgca
      361 tattatgaag gaaaaggaat caatccggga gaacttatcg atcttgcaga aacaatgccg
      421 ttttttgccg acagacgtct gatcgtaatc gaaaattccg gattcttcaa aaatgcgtca
      481 ccggagcttg cagattatat caaaacaatg ccggataccg cctgtttttt atttgtggaa
      541 aatgaagcag acaagcgtgg aaagatgtat aaggctgtca agagtaaagg acgcgtggta
      601 gagatggcac gtcaggatga gaagacactt ttatactggg ttgcagggaa tgtaaaaaaa
      661 gaaggatata agatcaaaga acagacggcc aggtatctgt tgtctaccac nnnnnnnnnn
      721 cagacaaagc tgaaaatcca gcagagtgaa aaagaactgg aaaagctgtt ctgttactgt
      781 atgggaaagg aagagattga agtccgggat atcgaagcaa tctgtacgac acagattaca
      841 aataaaatct ttgagatggt agaagcagtg gcggtacgga atcagaagaa agcactggat
      901 tattattatg atctgctggc attaaaggag ccgccaatgc gcatattata tttgttgaca
      961 aggcagttta aattacttct ggaagtcaaa gatctggcag gaaagcgcta tgacaaggct
     1021 gcgattgcaa agacagtcgg actgcatccg tttgttgcag gaaaatatat gcagcaatgc
     1081 cggacattcg aaaagaaaga actgagaagt attttggaag atgcagtaga tacggaagaa
     1141 ctggtcaaga ccggccgctt aaatgatgtg atgagcgtgg aacttttcat tgtaaaatac
     1201 agttctgtat gatatattca gataggtaat aacagagaaa attcaaacta aggggaacga
     1261 tagaattgtc aatggatcag agtaaaatca gaaatttttg tatcatagca tatatcgatc
     1321 acggtaaatc tacacttgcc gatcgtatta tagaaaaaac aggaaccttg actagcaggg
     1381 aaatgcaggc gcaggtgttg gataatatgg atctggaaag agagcggggc attacgatca
     1441 aggctcaggc agtccggacg gtttacaagg cttcggacgg agaagaatat atttttaatc
     1501 tgatcgacac cccgggacat gtcgatttta actacgaggt atcgagaagc cttgcggcat
     1561 gcgacggtgc gattctggtc gtggatgcgg cgcagggcgt ggaggcacag acccttgcga
     1621 atgtgtatct tgcgttagac catgatctgg atgtgatgcc ggtcatcaac aaggtcgacc
     1681 tgccgagcgc gcagccggat gaagttgtaa aggagatcga ggacgtcatc ggaatcgagg
     1741 cggaggatgc gccgcgcatt tccgcaaaga caggactcaa tgtcgatcag gtattagagc
     1801 agatcgtaca gaagattccg gcgccggccg gcgatccaaa agcgccgctt caggcgctga
     1861 tctttgacgc actctatgat tcctacaagg gtgtcatcgt gttctgccgg atcaaggagg
     1921 gcacggtgcg ggtcggcgat ccgatcctta tgatggcgac cggggcgcag tccgaagtta
     1981 ccgaggtcgg ttactttgga gcagggcagt tcattccgtg tgacgaactg tctgccggca
     2041 tggtcggcta catctgtgcg agcatcaaaa atgtgcggga tacccgcgtg ggtgatacgg
     2101 tgacgaacgc cgcacatcca tgtgaggagc cgcttccggg ttataaaaaa gtcaatccga
     2161 tggtatactg cggcatgtat ccggcggaca gtgccaaata tccggatctg cgcgatgcgt
     2221 tggagaagct tcagatcaac gatgcgtcgc tgcagtatga accggagacg tcgctggcgc
     2281 tgggcttcgg attccgttgc ggttttttag gactgcttca tctggagatc gtgcaggagc
     2341 gtctggagag agagtttcat ctggatctcg tgacgacggc accgggcgtt atctataagg
     2401 tgcacaagac gaacggagag gtgatcgatc tgacgaaccc gtccaatctg ccggatccgt
     2461 ccgagatcga gtatatggag gagccggtcg tttcggcgga gatcatggtg acgagcgagt
     2521 atgtcggcgc gattatgacg ctttgccagg aacggcgcgg tgtatacaac ggtatggagt
     2581 atctggagga gacccgtgcg ctcatcaagt atgatctgcc gctcaatgag attatctatg
     2641 atttctttga tgccttaaag tcgcgctcga gaggatacgc ttccttcgac tatgagctga
     2701 agggctatga gcgttccgag cttgtgaagc tggatatcct gattaacaag gagcaggtgg
     2761 acgcgctctc gttcatcgtc tttaaggaca gcgcttacga caggggcaga aagatgtgtg
     2821 agaagttaaa ggaagagatt ccgagacatc tgtttgagat tccgatccag gcggcagtcg
     2881 gcggcaaggt gattgcccgt gagacagtca aggcgctgcg caaggatgtg cttgcaaagt
     2941 gctacggcgg cgatatttcg cgtaagaaaa agctttcaga gaagcagaag gaaggcaaga
     3001 agcgcatgcg tcaggtcgga aatgtagaga ttccgcagga ggcattcatg agcgtcttga
     3061 agctggatga agattaaagg aacagatcgg aaaatgcagc gtcagtgatg gcagtgtcag
     3121 gaatattttt gggaaaggag cggcgtgtgg agtgagtcag aaaacaatgg aattgtacat
     3181 tcatattccg ttttgcgtca aaaaatgcca gtattgtgat ttcctttcct ttgcggcgga
     3241 cgaggcaacg caggcagcct acgtggaggc gctgctccgg gagattgcgt attacggacc
     3301 gcattgcagc gactacctgg tatcgacggt atatatcggc ggcggcacgc catcgtggct
     3361 cacagaggag tggatggcac agattatgtc ggcggttcgc cggtcgtttc gtctggcgtc
     3421 ggatgcggaa atatccattg agtgcaatcc gggaaccgtc accgatcaca agtttgccgg
     3481 ctaccggaat gcgggaatca accggatcag catcggcctg cagtcggctg tggatgagga
     3541 attaaagatt ctcgggcgga ttcacacgtt tgaccagttc ctgaagactt acgagctggc
     3601 gcgcaaaaac ggattcgaca atgtcaatgt ggatattatg agttctcttc cgggacagac
     3661 ggcagagagt tttgcccgca ccctgcagca gcttctgtgg ctgaaaccgg agcatatttc
     3721 cgcctattcc ctgatcattg agaaggggac gccattttac gatctgtata agtttgacgc
     3781 ggtcaagcag caggcgggaa tgcagacggt ggcgctgccg acggaggatg aagtgtaccg
     3841 gatgaccaag ctgaccgagc aggtgcttgc ggaagcggga tatgtgcact atgaggtttc
     3901 taattttgca aaaccgggtt atgcgtgccg gcacaatatc ggctactggg agcgggagaa
     3961 ttatctggga ctggggcttg gcgcgtcgtc tctgatggag aatatccgct acagcaacac
     4021 gcgggaattg tatgtgtatc tggaagaaac cagacatatt cgtgagggta tctgggagaa
     4081 tgagataacg gatagccagg ggagcggcgc atccggcagg gtggaacagc ttccggccac
     4141 gaatctgcat gcttcggcag agcgcattac acgaaaaggg cagatggaag agtttatgtt
     4201 tttaggactc cgcatgatgg agggaatttc cagggaacgt tttgcacaga cgtttggaac
     4261 acagattgag gcagtgtatc aggaggtgct gctgcatctt acggaggaag gactgctcga
     4321 aaaacgcgcg gggaggattt atttgacgga acgcgggcag gatgtgagca attacgctct
     4381 ggcacagttt ttactttaga acgatgaatc cggacggatt catattaaga aattcttcat
     4441 aaagattcat tcgcgttttc acggaaagag gactaaaata gaatcgtagg aatccaagga
     4501 gtgaagtgat gtgaatgtaa caaagagaca gaaaagacga attcggaatc tggtatgtat
     4561 acagtacagt attgtgaaaa tgctgctcgt gatcgcaggg tttgaactgt tgggacgggg
     4621 gctgtccctg atgcagggaa cgcagtctgc aacgcaggag attgagggag aggtaatctg
     4681 gtcggaggag acgaaaacag aggagaatcc cctgatcgtg tgcgtggatg ccggacatgg
     4741 cggaaaagac aacggctccg attataaggg gcgttatgag aaggatgata atttaaagct
     4801 ggcacaggct gtgtcagctt atttggctga taaaaatgta aaagtggtta tgacgaggtc
     4861 ggatgacacg tttttgcgcc tgtcagagcg gtgcgggatt gccaatcaga gcggtgcgga
     4921 ttatatgctg tcgctgcacc gcaatgacgg cgagggtaac ggcgtagaga cctggatctc
     4981 ggcgagtggt tcggaggaga cggatgccct cgcaaaccac attatggaag tgctggatac
     5041 ggcgggaatc cagagaaacc gcggcgtgaa aaagggaacc cagactggag aatcctccaa
     5101 ttattatatc aatgtacatt cggacatgcc gtcctgcatc gtcgagatgg gatttatcaa
     5161 cgatgcatcc gataaccagc ttttcgatga aaagctggat gcgtacgctg ccgccatcgg
     5221 tgacgcagtg cttgcaacgg cggagacata ccgtgccaac aaggttacgg atggaaacgg
     5281 tcagggcgca ggcgaagcgg gaagcggcac cggagacggt caaggtacgg atggaacggg
     5341 a
//
LOCUS       DBMDQJ010000067         5338 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6059_length_5338_cov_1.542104, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000067 DBMDQJ010000000
VERSION     DBMDQJ010000067.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6059_length_5338_cov_1.542104"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="ACZO9R_02220"
     CDS             <1..798
                     /locus_tag="ACZO9R_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR0911920.1"
                     /translation="DYDSSIIPLYTNYADGWPMEQWDGQIAGTTTGDSTYMNQKLLHT
                     KDPFQDYGDNTHPYALYKVLYDAVADGLTEDDFTTTSWEDSKGMINRGEIATMVLGSW
                     AYSQMVDADSHGEDIGYMPFPITIDGKQYASAGADYSFGINAASDADHQAAAMVFVKW
                     MTEESGFAYNEGGIPIAADDNDYPDAYAEFGDVTFVSDESALEGEEDLLNALNADSGL
                     NINAGGKEKVQEIIEHASNGDMSYDDIMAEWNEKWTQAQEDNGVTAE"
     gene            890..1936
                     /locus_tag="ACZO9R_02225"
     CDS             890..1936
                     /locus_tag="ACZO9R_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0911921.1"
                     /translation="MAATSVKKVENKTARRGFLKWAKSRKGQQTLIILAFTIIPLVLL
                     AAFTYVPFAEMVKFSFYKMKYSTPVDKRVFVGWKNYIEVFRNKDIFASLWLSLYYMVG
                     SVVQLALALFLATILSFKTRGGNLFKGLMFFPYLISGIAIGFIFKYFYTRGFVFDSIL
                     GWFGFELDSLPYWLRDQKINNWSLVATSVWRYFGNNMVLFIGAIMSVDSEMYEAAELD
                     GANKFQQFVHIILPSIKTIVTLNVILSITGALSAFEQPFVITGGSNGTATYFINMNNI
                     AHTSQKVGLASAMAVVLLLIIFACTILQKLFFKYVYRNADQDDEVMTREERKRLKQLH
                     KEQRAAKKAAKGAM"
     gene            1937..2791
                     /locus_tag="ACZO9R_02230"
     CDS             1937..2791
                     /locus_tag="ACZO9R_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0911922.1"
                     /translation="MKKRRKQYSAGAIIWIIFKYVSLVFFSFVAVIPIISCVITAFKT
                     EEEYKTTNVMTLPQNWFNFDNFVEAFQRANMGRAFVNSVIVMVCVLIGAIIIGTQLAY
                     VLNRFQFPGNGLIRNLFLFAALLPGVAMQVTVYQIMHDLGFINHLYGYIIMMMGTDVI
                     SIYIFIQYMENIPVSLDESAIIDGASYWKIYWKIMLPLLKPAIVTACILKGVGVYNEY
                     YSANLYLTKENLKTMAISLYTFTGPLGSQYNLICAGVIISLLPALIVFIVFQKQIYSG
                     ITAGAVKG"
     gene            2814..4046
                     /locus_tag="ACZO9R_02235"
     CDS             2814..4046
                     /locus_tag="ACZO9R_02235"
                     /EC_number="5.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HIR0911923.1"
                     /translation="METLESMRGEVKTELLTHIIPFWRALRDDTYGGYYGWLSYDLAL
                     DQKAEKGCILNSRITWFFSNAYTAYRKGLFTEEDCRSAGYTGEALLEEAKHGYVFLKE
                     HCIDREYGGIYWSLNYDGTPLDTTKHTYNQAFCIYALSSYYEASHDEEALALAKQLFS
                     LSEEKCTDEVGYLEAFTREFQPESNEKLSENGVLADKTMNTLLHVFEAYTELYRVSRD
                     ETVGERLRWIMDVFAEKVYNPAKRRQEVFFDAGYHTILDLHSYGHDIETAWLLDRGVK
                     VLGDEAYEAKIGPITQALAAHIYETAFDGHSLANECDRGVVNTHRVWWVQAETVVGFL
                     NACEQMPDHSEYRDAALAEWQFIKEHVIDPRRGSEWYWEVYEDGSPILDRPIVEPWKC
                     PYHNGRMCIEVLGRIKEN"
     gene            4059..5234
                     /locus_tag="ACZO9R_02240"
     CDS             4059..5234
                     /locus_tag="ACZO9R_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR0911924.1"
                     /translation="MLHERYYIEKEKQEKLLARKNEKSDFYNGVFDRYEYPVLTREHI
                     PLTWRYDLNPDTNPYFMERLGINAVMNSGAIELNGKYYLVARIEGNDRKSFFGVAESD
                     NGIDGFRFWDYPILLPDTCPEETNVYDMRLTKHEDGYIYGVFCSESKDTSVSDLSAAV
                     AAAGIVRTKDLKTWERLPNLITLRSPQQRNVVLHPEFVDGKYAFYTRPMDDFIETGSG
                     GGIGFGLCEDITHAVIDEEKMTSLRKYHTITEAKNGAGATPIKTEKGWIHIAHGVRNT
                     AAGLRYVIYAFATDLKDPSKVIAEPSGLLIGPRGEERVGDVSNVVFTNGAVVNEKNEV
                     FIYYASSDTRMHVATTTVEKLVDYVFHTPSDPGRSVECVAQRCGLIEKNLAFLKANE"
BASE COUNT         1429 a         1156 c         1492 g         1261 t
ORIGIN      
        1 gattacgatt cttccatcat tccgctctac acgaactatg cagacggatg gccgatggag
       61 cagtgggacg gacagatcgc aggtacgaca accggtgatt ctacctatat gaaccagaag
      121 cttcttcaca caaaggatcc gttccaggat tacggcgata acacacatcc gtatgcactg
      181 tacaaggtac tctacgatgc agtggcagac ggactgacag aggatgactt caccacaacg
      241 tcctgggaag attccaaggg catgatcaac agaggcgaga tcgcgaccat ggtacttggc
      301 tcctgggcat attcccagat ggtagacgct gattctcatg gcgaggatat cggctacatg
      361 ccattcccga tcacgatcga cggcaagcag tatgcttccg caggcgctga ctacagcttc
      421 ggtatcaatg cggcatcgga tgcagatcat caggcagcag caatggtatt cgtaaaatgg
      481 atgacagaag agtccggttt tgcatacaac gagggcggta tcccgatcgc tgcagacgat
      541 aacgattatc cggatgcata cgcagagttc ggtgatgtga catttgtatc cgacgagtcc
      601 gcattagagg gcgaggaaga tttactcaat gcattgaacg ctgattccgg attgaacatc
      661 aatgcaggcg gaaaagagaa ggttcaggag atcattgagc atgcttctaa cggtgatatg
      721 tcctacgatg acatcatggc tgagtggaat gagaagtgga cacaggcaca ggaagacaat
      781 ggcgtgacgg cagaataaat aagacatcat gcacagacgg gaccggaatg agagatctgt
      841 ttccggtccc gttattttaa aaacagggtg aaaaagatgg aggttggata tggcggcgac
      901 atcagtaaag aaagtagaaa ataaaacagc caggcgtgga ttcctgaagt gggcaaaaag
      961 caggaagggg cagcagacac tgattatact ggcatttacc ataattccgc ttgtgctgtt
     1021 ggcagcattt acctatgtgc catttgctga aatggtgaag ttcagttttt acaaaatgaa
     1081 atattccacg ccggtagata aacgggtatt tgtgggttgg aagaattata tcgaggtatt
     1141 ccgcaacaag gatatttttg catccctgtg gttaagcctc tactacatgg taggttcggt
     1201 ggtacagctt gcgcttgcgc tgttccttgc aacgattttg agctttaaga caaggggagg
     1261 caatctcttt aagggactga tgtttttccc gtatctgatc agcggtattg cgatcggatt
     1321 tatctttaaa tatttttaca caagaggatt tgtattcgat tccatcctcg gatggtttgg
     1381 atttgaactg gatagtcttc cgtactggct gcgcgatcag aagatcaaca actggtcact
     1441 ggttgcaact tctgtctgga gatatttcgg caacaacatg gtgcttttta tcggagcgat
     1501 tatgtctgta gattccgaga tgtacgaggc ggcggagctg gacggtgcaa ataaatttca
     1561 gcagtttgta catatcatac tgccgagcat caagacgatt gtgacgctga atgtgatttt
     1621 atccatcaca ggagcactga gcgcattcga gcagccgttc gtaattaccg gaggttccaa
     1681 cggaacggca acgtatttta tcaacatgaa caatattgca cataccagcc agaaggtcgg
     1741 actcgcgtcg gcgatggcgg ttgtgttact tcttatcatt ttcgcatgta cgatcttaca
     1801 gaaactgttc tttaaatatg tataccgcaa tgcggatcag gacgatgaag tcatgaccag
     1861 ggaagagaga aagcggttga aacagcttca caaagaacag agagcggcga aaaaagcagc
     1921 aaagggggcg atgtaaatga aaaagcgcag aaaacagtat tcggcaggag ccattatctg
     1981 gatcattttt aaatatgtat cgcttgtgtt tttttccttt gtcgccgtga ttcccattat
     2041 ttcctgcgtc atcactgcat tcaagacgga ggaagagtat aagacgacga atgttatgac
     2101 actgccgcag aactggttca attttgacaa ctttgtggaa gcgttccagc gtgcgaacat
     2161 gggaagggca ttcgtgaatt ccgtgatcgt tatggtctgt gtcctgatcg gagccatcat
     2221 catcggtaca cagcttgcgt atgtgttaaa ccgtttccag ttcccgggca acgggctgat
     2281 ccgcaacctg tttttattcg cggcactgct tccgggtgtt gccatgcagg taacggtcta
     2341 ccagatcatg catgatcttg gctttatcaa tcatctgtac ggctacatca tcatgatgat
     2401 gggaacggat gtcatctcca tctacatctt tatccagtac atggaaaata ttccggtgtc
     2461 gctggatgag tcggcaatca tcgacggagc gtcctactgg aagatctact ggaagatcat
     2521 gcttccgctg ctaaagccgg caattgtgac cgcctgcatc ttaaagggcg tgggcgtgta
     2581 caacgaatac tattcggcaa acctgtatct gaccaaggaa aacttaaaga cgatggcgat
     2641 ttctctctat acctttaccg gaccgcttgg aagccagtac aacctgatct gtgcaggtgt
     2701 tatcatttcc ctgctgccgg cacttatcgt gtttatcgta ttccagaagc agatctacag
     2761 cggtattacg gcaggcgcag tcaagggctg attacaggaa aagaggaaca gcgatggaaa
     2821 cattagaaag catgcgagga gaagttaaaa cggaacttct cacacatatc attcccttct
     2881 ggcgtgcact gcgtgacgac acttacggcg gctattacgg ctggctgtct tatgatcttg
     2941 cactggatca gaaagcggaa aaaggatgca ttttaaacag ccgcatcaca tggtttttct
     3001 ctaatgccta cacggcatat cgaaagggac tttttacgga ggaagactgt agaagcgcag
     3061 gctataccgg agaggcgctc ttggaggaag caaaacacgg ctacgtcttt ttgaaagagc
     3121 actgcatcga ccgggagtac ggcggcatct actggtcatt aaactatgac gggacaccgc
     3181 ttgatacgac aaagcacacc tacaaccagg cgttttgcat ctatgcattg tcctcctact
     3241 atgaggcgtc gcacgacgag gaagcgcttg cactggcgaa gcagctcttt tccctgagcg
     3301 aagagaagtg tacggatgag gtcggatacc tggaggcttt taccagagag ttccagccgg
     3361 aatccaacga gaagctgtcg gagaacggcg tgcttgcgga taagacgatg aacaccctgc
     3421 ttcatgtttt tgaggcgtac acagagctgt accgtgtgtc gcgggatgag accgtggggg
     3481 agcgcctccg ttggatcatg gatgtgtttg cagagaaagt atacaatccg gcgaagcgga
     3541 gacaggaagt gtttttcgat gccgggtatc acacaatctt agatctgcat tcctacgggc
     3601 atgacattga gacagcgtgg cttttggacc gcggcgtcaa ggtgctgggc gatgaggcgt
     3661 atgaggcgaa aattggtccg atcacacagg cgcttgcggc acatatttat gagacggcgt
     3721 ttgacggaca ctccctggca aatgagtgtg acaggggcgt agtcaataca caccgtgtat
     3781 ggtgggtgca ggcggagaca gtagtcggat ttttaaatgc ctgtgaacag atgccggatc
     3841 attcggagta ccgggatgcg gcgttggcgg agtggcagtt catcaaagaa catgtcattg
     3901 atcctcgcag gggaagcgag tggtactggg aagtgtatga ggacggaagc ccgattttag
     3961 accggccgat cgtggagccg tggaaatgtc cgtatcacaa cgggcgcatg tgtattgagg
     4021 tactgggacg catcaaggaa aattgaggag aattggttat gctgcatgag agatattata
     4081 tagaaaaaga aaagcaggaa aaactgcttg cacggaaaaa cgagaaatcg gatttttaca
     4141 acggcgtatt tgaccgttac gagtatccgg ttctcacaag agagcatatt ccgctgacct
     4201 ggcggtacga tttaaacccg gacaccaatc cgtactttat ggagcgtctc ggcatcaacg
     4261 ccgtgatgaa ctccggagcg atcgagctga acgggaaata ttatctggtg gcgcgcatcg
     4321 aggggaatga cagaaagtct ttcttcggcg tggcggagag cgacaacgga atcgacggtt
     4381 tccgtttctg ggattacccg attcttctgc cggatacctg ccctgaggag acgaatgtct
     4441 acgatatgcg cttaacgaag catgaggacg gttacatcta tggcgtgttc tgctccgagt
     4501 caaaggatac gagtgtgagc gatctctcgg cggcagtggc ggcagccgga atcgtccgca
     4561 cgaaggattt aaagacctgg gagcgcctgc cgaacctcat cacgctgcgt tccccgcagc
     4621 agagaaacgt ggtgctgcat ccggaattcg tcgacggcaa atatgcgttt tacacgagac
     4681 cgatggatga ttttatcgag acgggaagcg gcggaggcat cggattcggt ctctgcgagg
     4741 acatcacaca cgctgtgatc gatgaggaaa aaatgacgag tctgcgcaag tatcatacga
     4801 tcacggaggc gaagaacgga gccggtgcga cgccgatcaa gacggaaaag ggatggattc
     4861 atatcgctca cggtgtgcgc aacacggcag cgggactgcg ttatgtcatc tatgcgtttg
     4921 caacggactt aaaagatccg tcgaaggtga ttgccgagcc gtccgggctt ctgatcggac
     4981 cgagaggtga ggagcgcgtc ggggatgtca gcaatgtggt attcaccaac ggagcggtcg
     5041 tcaatgagaa aaacgaggtg tttatttact acgcatcctc ggatacacgg atgcatgtgg
     5101 cgaccacgac ggtggagaag ctggtggact acgtattcca tacgccgtcc gatccgggaa
     5161 gatcggtgga gtgtgtggca cagcggtgcg gtctcatcga gaagaatctt gcatttttaa
     5221 aagcgaatga ataaatacgg tcatcataga aaaatgagcc gcggcggcgg cagaaagagg
     5281 tagaagttat gagcaaatat aaaatgatca gccccaaagt accgaatgtg ccatggca
//
LOCUS       DBMDQJ010000068         5281 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6120_length_5281_cov_1.635907, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000068 DBMDQJ010000000
VERSION     DBMDQJ010000068.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5281)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_5281_cov_1.635907"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="ACZO9R_02245"
     CDS             <1..486
                     /locus_tag="ACZO9R_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR0911925.1"
                     /translation="NIIGIPLAAGVWIPLFGWQLNPMFGAAAMSLSSCCVVTNALRLN
                     LFRMYDAGKDRRIKNAVTGQTAAAAAKEPDDALEKKSETNQKKENETMKKTMKINGMM
                     CGHCEARVKKALEAVEGVQEAVVSHESGNAVVTLTSDVADEVLKKAVEDQDYEVVSVE
                     S"
     gene            556..2001
                     /locus_tag="ACZO9R_02250"
     CDS             556..2001
                     /locus_tag="ACZO9R_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR0911926.1"
                     /translation="MRYVPLKKAEPGMVLAADLYDSVGRTLIGCHCELTESYLEKLEA
                     YGFDGVYIEDKLSEGITIESVITPQLRQEGQERIRACDIDGCKLIARRMVEEILSCGN
                     VSLDLTDLRSYDDYTYAHSVNVAVYCGVIGMGMGMSEVELGHLVTAALLHDLGKLQIP
                     DEILNKPGRLTQEEYLIMKSHATLSYQIISERWDISAHIKEAVLHHHENVDGSGYPDG
                     LEGAQQTMFTRILHVADVYDALTSRRPYKEPYAPYEATEYLMGGCGIMFDREVVETLL
                     KYVPLYPKGTMVTLSDGREAIIYENFGVHNLRPVVRLMDGELLDLSNEANYHITLRMK
                     TESGFSTEEAEKERNEMIRPPVRCRIMVVDDMKTNLQMLRGILEPLYDVILMKSGHQA
                     LLYLKKHPAPDLVLMDIDMPEMDGIETAKRIMEMTDHTVPILFVTALCDRQTVTLCRN
                     LDAAGYIVRPYKPVFVKTEIKRILMGRSEIE"
     gene            1998..4697
                     /locus_tag="ACZO9R_02255"
     CDS             1998..4697
                     /locus_tag="ACZO9R_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0911927.1"
                     /translation="MILLVQIICLCYTLYVLQEYATSGAYSRQHRLLPLLVGLIGVYN
                     FYQVVLTLTGQTELFGRLKDMLLIQVLYLILIYIMDFLKIHIPYGVQWIMFCVLLALN
                     VIVFMRYRQPEIYHIYFRIYLIGCTAAIVAMGTYAYLRRSLTMHEHQIANLLYIVLLV
                     NAAAMCAEKFFTIPGNLLMLAATTGVCGVVHYLIQTDQLMDTGELLRERLYDDSDTAV
                     VLLDTNFYCVDANRSGRSLFAGPMQLYERDSRRHRGYMQEAEELAKSIQEGKKSREIE
                     KNGQYYQCQCTPVYYSGRLRGYILGAWDITSPKKETQLMQALKGKAEMQTARKSDFLA
                     QMSHDLKSPLHAIIGITDILSSRKELTASDRALLLHIKGAGNSLVEQVNAILDYSRIE
                     AGKFELMAECYRLDQVLEEVAHMCVINLQSKRVWFEACIQGEFPEKMYGDAMRVREIL
                     QNLLANAVKFTEEGEIRCVITCEAIRDSSDVMVRCRVSDTGTGMTSEQLRQGFDSYVS
                     YAAESGQSGNGLGLSIVKELANRMGGSVRAESNGTRGTTITVEIRQKVCGSTLHDAVC
                     YTTDSLLRESVVYTERIQPTWIYPGAHVLLADDMRINQEIFQELAAPWRFTLDVVRNG
                     KEAVEAAARKEYQLIFLDQRMPEKNGDEAAKEIRQFCDIPMVLMTADITAELKHRNRR
                     PEFTAYLDKPIDVSELKKVIETYLPVAERRDFLLHAQMNHGAYRRLLQTFLQETGSLA
                     AELPGYVPEQLELFGTKVHGIKGASQQIGRQALSESAEIMEMAAKTENIRYMEKHMTE
                     FLETIRHTLESVEDELKNLPQEIQGEGAATAEDIPEEKQQILAQLREAFDACDLNGIE
                     QGLSRFARAHLTAEERELLTELHAAYENLDYEEGSELLSGRML"
     gene            4856..>5281
                     /locus_tag="ACZO9R_02260"
     CDS             4856..>5281
                     /locus_tag="ACZO9R_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014902.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HIR0911928.1"
                     /translation="MNLAEITHRAYFLDCYPLDERHLKISIQTGKDVDEVILWAGDPY
                     SAGIAENEQNWQGERVPMELERELAHRNVYSAVLTPAYRRVKYHFEITGGGESVCLYE
                     DGFVKAGENFPADRMIQRFIYPWMNRADIMKVPEWAENIV"
BASE COUNT         1370 a         1105 c         1630 g         1176 t
ORIGIN      
        1 aatattatcg gcattcctct tgcggcaggc gtgtggattc cgctgttcgg ctggcagtta
       61 aacccgatgt tcggtgcggc agccatgagc ttatccagct gctgtgttgt gaccaacgcg
      121 ctgcgcctga atctgttccg gatgtatgac gccggaaaag accgcaggat caaaaatgcg
      181 gtgacagggc agaccgccgc agctgcggcg aaggagccgg atgacgcttt ggagaagaaa
      241 tcagaaacaa atcaaaaaaa ggagaatgag acaatgaaaa agacaatgaa gattaacggt
      301 atgatgtgtg gacactgtga ggcgagagtc aagaaggcac tcgaagcggt agaaggcgtg
      361 caggaggctg tagtcagcca cgagtcagga aacgcggttg tgacgcttac cagcgacgtg
      421 gcggatgagg tcttaaagaa ggcggttgag gatcaggatt acgaggtggt ttctgtcgaa
      481 tcctagaaaa taaggtcaaa agtttcattg actttatgag gtcggcttgg tacagtgaaa
      541 ggtaggagga atgttatgcg ttatgtacca ctcaagaagg cagaaccggg catggttctt
      601 gcagccgacc tttatgattc cgtcggaaga acgctgattg gctgccattg cgaattgacg
      661 gagagctatc tggaaaaact ggaagcttac ggattcgacg gtgtatatat agaagataaa
      721 ctttcagagg gcatcacgat tgagagtgtg attacgccgc agttaaggca ggagggacag
      781 gagcgcatcc gtgcctgcga cattgacgga tgtaagctga ttgccagacg catggtggag
      841 gagattttat cctgcggaaa tgtgtctctt gatcttacgg acttaagaag ctacgacgat
      901 tacacttacg cacattctgt gaacgtggcg gtatactgcg gcgtcattgg aatgggtatg
      961 gggatgagtg aagtggagct ggggcatctt gtgactgcgg cattgcttca tgacctcgga
     1021 aagctgcaga tcccggatga gatcttaaac aagccgggac gtctgacaca ggaagaatat
     1081 ctgattatga aatcccacgc aacactgtcg taccagatta tcagtgagcg gtgggatatt
     1141 tctgcgcata tcaaggaggc cgtgctgcat caccatgaaa atgtggacgg aagcggatac
     1201 ccggacgggc tggaaggcgc gcagcagacg atgtttacca ggattctgca cgtggcggat
     1261 gtctatgatg cacttacgtc caggcgcccg tacaaggagc catacgcacc gtacgaggcg
     1321 acagagtatc tgatgggcgg ctgcggcatc atgtttgacc gcgaggtagt ggagacactt
     1381 ctaaagtatg tgccgctcta tccgaaggga acgatggtta cgctttccga tggaagagaa
     1441 gccatcatct atgagaattt tggcgtacat aatttaagac ccgtagtgcg tctgatggac
     1501 ggggaactgt tggatctgtc caacgaagcg aactaccata ttacattgcg tatgaagacg
     1561 gaatccggct tctccacgga agaggcagaa aaggagcgca acgagatgat acgcccgccg
     1621 gtacgctgcc ggatcatggt tgtggacgat atgaagacga atctgcagat gctgcgtggc
     1681 attttagagc cgttatatga tgtcatcctg atgaaatccg gtcaccaggc attgctgtat
     1741 ttgaaaaaac atccggcgcc ggatctggtg ctgatggata tcgatatgcc ggagatggac
     1801 ggtatcgaga cggccaaacg cattatggag atgacggatc acacggtgcc gatcctgttc
     1861 gtcacggcgc tctgcgacag acagacggtc acgctgtgcc ggaatctgga tgcggcaggt
     1921 tacattgtaa gaccgtacaa gccggtcttt gtcaagacag agattaagag gattctgatg
     1981 gggcggagtg agattgaatg atattattgg ttcagataat atgtctttgt tatacgctgt
     2041 atgtactgca ggaatatgcc acttccggtg catattccag gcagcacagg ctgctgccgc
     2101 tgctggtcgg actgatcggt gtatataatt tctatcaggt tgtccttacg ctcaccggac
     2161 agacagaact gttcgggcgt ttgaaggaca tgctcttaat tcaggtgctt tatctgattc
     2221 ttatctatat catggatttt ctgaaaatcc atatccccta cggggtgcag tggattatgt
     2281 tttgtgtgct gctcgcgctg aatgtgattg tttttatgcg ctaccggcag ccggaaatct
     2341 atcacattta tttcagaatt tacttaatcg gatgtacggc ggcgatcgtt gcaatgggaa
     2401 cctacgccta tctgcgccgt tctctgacca tgcatgagca tcagattgct aatctgctct
     2461 atattgtgct gctcgtgaat gcagcagcga tgtgcgcgga aaagtttttt actattccgg
     2521 gcaatcttct gatgctggca gctacaaccg gtgtctgcgg tgtggttcat tatctgatcc
     2581 agacggatca gctgatggat acgggcgagc ttctgcgtga gcggctgtac gatgactcgg
     2641 atacggcagt ggtactgctg gataccaatt tttactgcgt tgatgcgaac cgcagcggca
     2701 gaagtctgtt tgcgggtccg atgcagttgt atgagagaga cagccgcagg caccgcggat
     2761 acatgcagga ggcggaggag ctggcgaagt ccatacagga gggaaaaaag agccgggaga
     2821 tcgagaagaa cggacagtat tatcagtgtc agtgtacgcc ggtgtactat tcagggaggc
     2881 ttcgtggata tattctcggt gcctgggata ttaccagccc gaaaaaagag acgcagctga
     2941 tgcaggcatt aaagggaaaa gcagagatgc agacggcgcg gaaaagtgat ttcctcgcgc
     3001 agatgagcca tgacctgaaa agcccgctgc acgccatcat cggaatcacg gatattttaa
     3061 gcagccgcaa ggaactcacg gcaagtgacc gtgcactcct gcttcacatc aagggagctg
     3121 ggaacagcct tgtggaacag gtgaatgcga ttcttgacta ttccagaatt gaggcaggaa
     3181 agttcgagct gatggcggag tgctaccggc tggatcaggt gctcgaggag gtggcacaca
     3241 tgtgtgtcat caatctgcag tcgaagaggg tgtggtttga ggcctgtata cagggagaat
     3301 tcccggagaa aatgtatggg gatgccatgc gcgtccggga aattttgcag aatctgctcg
     3361 caaatgcggt caagttcaca gaggagggcg agatccgctg tgtgattacc tgtgaggcaa
     3421 tccgggattc ctcggatgtg atggtccgct gcagggtgtc ggacaccggc accggaatga
     3481 caagcgaaca gctgcggcag ggatttgatt cgtatgtgag ctatgcggca gagagcggac
     3541 agtcgggaaa cggactggga cttagcattg taaaggaact tgcaaaccgt atgggaggca
     3601 gtgtccgcgc cgagagcaac ggcaccaggg gaaccacgat taccgtggaa atccggcaga
     3661 aggtgtgcgg aagcaccctg catgatgcgg tgtgctatac gacggacagt ctgctgcgcg
     3721 agtcagtggt ctatacggag cggatccagc cgacatggat ctatccgggg gcacatgttc
     3781 tgctcgcgga tgatatgcgg atcaatcagg agattttcca ggaactcgca gcgccgtggc
     3841 ggtttacact ggatgttgtg cgcaacggaa aggaggctgt ggaggcggca gcgcggaagg
     3901 agtatcagct gatttttctg gatcagagga tgccggaaaa aaatggcgat gaagcggcga
     3961 aggagatccg ccagttttgc gatatcccaa tggtacttat gactgctgat attaccgcgg
     4021 agttaaagca tcggaatcgc agaccggaat ttacggcgta tctggacaag ccgatcgatg
     4081 tgtcagagct aaagaaagtg attgagactt atctgcctgt cgcagagcgc agggatttcc
     4141 tgcttcacgc gcagatgaac catggggcat accgccgtct cttgcagacg tttttgcagg
     4201 agacgggatc gcttgcagca gaactgccgg gctatgtgcc ggagcagctg gagcttttcg
     4261 gaacgaaagt gcacggaatc aagggtgcca gccagcagat cggcagacag gcgttaagcg
     4321 agagcgcaga aatcatggaa atggcggcaa agacagaaaa tatccggtac atggaaaagc
     4381 atatgacaga gtttttggag acgatccggc acacgttaga gagtgtcgag gatgaattaa
     4441 agaatctgcc gcaggagata cagggggaag gcgcagccac ggcagaggac ataccggagg
     4501 aaaagcagca gattctggcg cagcttaggg aagcttttga tgcgtgtgat ttaaacggga
     4561 ttgagcaggg actttcccgg tttgcgcgtg cccatctgac agcggaggaa cgggaactgt
     4621 tgacagaact gcatgcggca tatgagaacc tggattacga agagggaagc gaactgcttt
     4681 ccggccggat gctgtgatag aagtgaaaga aaaaatgatt gttttgtaca ggttacgcga
     4741 ttgatagtgt gagaaaaatt cccctcgcag agatatactg tgcgtagaat agaaatccgg
     4801 aattcccgga gcagctgaca ggagatctgc agcaaatttg tacaggggga aaactatgaa
     4861 tctggcagaa attacacatc gggcgtattt tttagactgc tatccgcttg atgagagaca
     4921 cttaaaaata agtattcaga caggaaaaga tgtggacgag gtgatcctct gggcgggcga
     4981 cccgtattct gccgggattg ccgagaacga gcagaactgg caaggcgagc gggtgccgat
     5041 ggaactggag cgggaactgg cgcacagaaa tgtatatagt gccgtcctca caccggcgta
     5101 ccgcagagtc aagtaccatt ttgagatcac cggaggcggg gagagcgtct gcctgtatga
     5161 ggatggattt gtgaaagcgg gagagaattt tcctgcggac cggatgatcc agcgttttat
     5221 ctatccgtgg atgaaccggg cggacattat gaaggtgccg gaatgggcgg agaatatcgt
     5281 g
//
LOCUS       DBMDQJ010000069         5278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6130_length_5278_cov_1.599382, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000069 DBMDQJ010000000
VERSION     DBMDQJ010000069.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6130_length_5278_cov_1.599382"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /gene="recN"
                     /locus_tag="ACZO9R_02265"
     CDS             complement(<1..1568)
                     /gene="recN"
                     /locus_tag="ACZO9R_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR0911929.1"
                     /translation="MLQSLHVKNLALIDEAEVDFTRGLNILSGETGAGKSIIIGSINL
                     ALGEKVQKEMLREDPQTGEFAPALVELVFTVENGQERQKLEALEVYPEDDQVILSRRI
                     VGGRGTARVNGQSMPASAVREIAAILIDIHGQHEHQSLLSKRRHLEILDAYVGETLTE
                     KKKALAETYRSYKKLVEEEKNAGIDGAEREREISFLEYEIREIEEAGLREGEDEELEN
                     EYRRFSNGQKIVNAVNGAYSNTGGESDCASELTGRAVRELSSVSGYDEKLAELERELL
                     EIDGLLSDFNRELADYVENMEYDEETFYQVEKRLDEVNHLKSKYGSTIPDVLLALEEK
                     QERLMQLKDYDTYLAGLKEEIARTKKVLEKQSSEVSAMRKEAAERLTAAVTQALLDLN
                     FLDVRFTMNFERMAEYTANGFDDAEFLISTNPGEPLKPLGKVASGGELSRIMLAIKTV
                     LADEDAVETLIFDEIDSGISGRTAQMVSEKMNVLGRSHQIICITHLPQIAAMADSHYL
                     IQKSVQNQTTVSTIR"
     gene            complement(1604..2056)
                     /gene="argR"
                     /locus_tag="ACZO9R_02270"
     CDS             complement(1604..2056)
                     /gene="argR"
                     /locus_tag="ACZO9R_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HIR0911930.1"
                     /translation="MKTKRQAKMLELIKRNDIETQEELSDYLEREGYQVTQATVSRDI
                     RELKLTKVAMSNGRQKYVALQETDENLLQKYVRVFHDGFVSMDMAQNILVIKTVSGMA
                     MAVAAALDAMHMHEIVGCIAGDDTIMCAIRSVDDTVAVMNRLKKLVED"
     gene            complement(2076..2969)
                     /locus_tag="ACZO9R_02275"
     CDS             complement(2076..2969)
                     /locus_tag="ACZO9R_02275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR0911931.1"
                     /translation="MTGRLPDVGKLMRHFLIITNTYKDENGRLTGELAAYIRKKGGEC
                     DCFYSDGESKSEAAPALDKMDPATDCVMVLGGDGTLIRAASRLVDSRIPLIGVNLGTV
                     GYLCELEESNVFDAVDRLMADDCMVEERMMLTGAGILGGVREEYGVALNDIVIHRTGE
                     LSVVSLIVYVNGEYLHTFRADGIIVSTPTGSTGYNMSAGGPIVDPKAQMILITPINAH
                     NLNSRSIVIGAEDEVVVEIGRRRSQKDETLEVSFDGDTAARLVVGDKFSIRKASDTTR
                     ILKLSNKSFLEILSKKMQVYT"
     gene            complement(2984..3787)
                     /locus_tag="ACZO9R_02280"
     CDS             complement(2984..3787)
                     /locus_tag="ACZO9R_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HIR0911932.1"
                     /translation="MKERLDVLLVNRGLAPSREKAKTMIMEGNVFVENQREDKAGATF
                     DTEAEITVKGNTLKYVSRGGLKLEKAMTHFGITLEGKVCMDIGASTGGFTDCMLQNGA
                     VKVYSVDVGYGQFAWKLRQDPRVVCMEKTNIRYVTPADIDDVLDFASVDVSFISLTKV
                     LIPARELLRDGGEMVCLIKPQFEAGREKVGKKGVVRDKAVHEEVVERIIEFASQNGFF
                     VKNLEYSPIKGPEGNIEYLVYIRKDETGGVDAAVDIKAVVDAAHGELDK"
     gene            complement(3784..>4904)
                     /locus_tag="ACZO9R_02285"
     CDS             complement(3784..>4904)
                     /locus_tag="ACZO9R_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR0911933.1"
                     /translation="LQEASRVDGPVLVHVITHKGTGYAPAERHPARFHGTEPFDIETG
                     LPKHPRTKANYTDVFSTVMRKLGDRDERVVAITAAMTDGTGLKRFHNMFPERFFDVGI
                     AEQHAVTFAAGLAAGGLKPIFAVYSSFLQRAYDQILHDVCIQNLPVVFAIDRAGLVGS
                     DGETHQGIFDISYLSTIPNMTIMAPKNKWELSDMIKYAVDFSAPIAIRYPRGEAYDGL
                     REYRAPIACGKSEWIYRSGQIALFALGAMVKTAEAVHERLKALGYESSLINARFAKPL
                     DEDALLSAARHHDLIVTMEENVISGGMGEQVMRFYEEHNLSVRVLAIAIPDEYVEHGN
                     VEILRREVGIDAETITERTIEAYEELHAQESGAERNDF"
     assembly_gap    4905..4914
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4915..>5278)
                     /locus_tag="ACZO9R_02290"
     CDS             complement(<4915..>5278)
                     /locus_tag="ACZO9R_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HIR0911934.1"
                     /translation="AHAREITGENYKVVSVIGDGALTGGMAFEALNNAAQLNSNFIIV
                     LNDNNMSISENVGGLSTYLAGFRTADAYRDLKLGVMNSLNKIPVYGDKMVERIRRTKS
                     GIKQLFIPGMLFEELGII"
BASE COUNT         1109 a         1707 c         1128 g         1324 t
ORIGIN      
        1 cggatcgtag agactgtcgt ctgattctgc acagatttct gaatcagata atgggaatcc
       61 gccatcgccg caatctgcgg gagatgtgtg atacagataa tctggtgcga ccgtcccagc
      121 acgttcatct tctcggaaac catctgcgcc gtccggccgc tgattccgct gtcgatctca
      181 tcaaaaatca gcgtctccac cgcatcctca tccgctaaca ccgtcttgat ggcaagcatg
      241 atacgcgaaa gttctcctcc cgacgctact tttccgagcg gttttaacgg ctcgcccggg
      301 ttcgtggaaa tcaaaaattc cgcatcgtcg aatccgttcg ccgtatactc tgccatgcgc
      361 tcaaaattca tcgtaaaacg cacatcaagg aaattcagat ccagtagcgc ctgcgtgacc
      421 gccgcggtaa gccgctctgc cgcctcctta cgcattgcgg acacctcgct gctctgcttc
      481 tctaagactt ttttagtccg cgcaatctct tccttaagac cggcgagata cgtgtcatag
      541 tccttaagct gcatcaggcg ttcctgcttc tcttctaatg ccagaagcac gtccgggatc
      601 gtgctgccgt atttggattt cagatgattc acctcatcga gacgcttctc cacctggtag
      661 aacgtctcct catcgtactc catattctcg acataatcgg caagttcccg gttgaaatcg
      721 cttaacagcc cgtcgatctc aagcagctca cgctcgagtt cggcaagctt ctcatcgtat
      781 ccggagactg acgagagctc gcgtaccgcc cttccggtca gctccgacgc acagtccgat
      841 tcgccgccgg tattgctgta tgcaccgttt acggcattca caatcttctg tccgttggaa
      901 aaccggcggt actcattctc cagttcctcg tcctcgcctt cccgaagtcc tgcctcctcg
      961 atctcacgga tctcgtactc caggaacgaa atctcccgct cgcgctctgc gccgtctatg
     1021 ccggcatttt tctcttcctc caccagcttt ttgtagctgc ggtaagtctc tgcgagcgcc
     1081 ttctttttct ctgtcagcgt ctctcccaca taggcatcca gaatctccaa atgtctgcgc
     1141 tttgaaagaa gcgactgatg ctcatgctga ccgtgaatgt caattaaaat cgccgcgatc
     1201 tcgcgcaccg cggatgccgg catactctga ccgttgacgc gcgccgttcc ccttccgcct
     1261 acgattctgc ggcttaggat cacctggtca tcttccggat agacctcgag cgcctccagc
     1321 ttttgccgtt cctgcccgtt ctccaccgta aataccagtt ctaccagcgc cggagcaaac
     1381 tccccggtct gcggatcttc tctcaacatt tccttttgca ctttttcacc cagcgccaga
     1441 ttgatcgaac cgatgatgat ggacttaccc gcaccggtct ctcccgagag gatattgagt
     1501 ccccgggtaa aatccacctc cgcctcgtca atcagcgcca gatttttcac atgcagactt
     1561 tgtaacatag aatctccttc tactctcccc aaaagaatcc cgtctagtct tccacaagct
     1621 ttttcaagcg gttcatcacc gcaacggtat catccacact gcggatcgcg cacatgatgg
     1681 tgtcatcccc cgcaatgcag cccacgatct cgtgcatatg catggcatct aacgcagccg
     1741 ccaccgccat cgccataccc gagacagtct taatgaccag aatattctgc gccatatcca
     1801 tggatacaaa tccgtcgtgg aacacgcgca catatttctg caacagattt tcgtccgtct
     1861 cctgcagcgc aacgtatttc tgcctgccgt tgctcattgc aaccttggtg agctttagtt
     1921 cccggatgtc cctggaaacc gtcgcctgtg ttacctgata gccctcacgc tccagataat
     1981 ccgacagttc ttcctgtgtt tcaatgtcat ttctcttgat cagttccagc attttcgcct
     2041 gtcgtttggt tttcattctg ccctccgttt cccgcttatg tgtatacctg catctttttg
     2101 cttaggatct cgagaaagct cttgttgctc agctttaaga tccttgtcgt gtccgaagcc
     2161 ttccggatac tgaacttatc tcccaccaca agtcttgcgg cggtatcccc gtcaaagctg
     2221 acctccagcg tctcatcctt ctgtgagcgc cttctgccga tctcaacaac cacttcgtcc
     2281 tccgcaccga tcacaatact gcgtgaattg agattatgcg cattgatcgg cgtaattaaa
     2341 atcatctgtg ccttcggatc cacgatcggt ccgcctgccg acatattgta gccggtagaa
     2401 ccggtcggcg tcgacacaat gattccgtcc gcccgaaacg tatgcagata ctccccgttc
     2461 acatagacga tcaggctgac taccgacaac tcccccgtgc ggtgaatcac gatatcattc
     2521 agggcaaccc cgtattcttc ccgcactccg ccaaggatgc ctgcgccggt gagcatcatg
     2581 cgctcctcca ccatacagtc atccgccatc agacggtcaa ccgcgtcaaa cacgttgctc
     2641 tcctcgagtt cacacagata accgaccgta ccaaggttca cgccgatcaa cggaatcctg
     2701 ctgtccacca gcctggatgc cgcgcggatc agcgttccgt cgccgcctaa caccatgacg
     2761 cagtccgtcg ctggatccat cttatccaaa gcaggcgccg cctcgctttt gctctccccg
     2821 tcactgtaaa agcagtcaca ttctccgccc tttttgcgga tatacgccgc aagttctccg
     2881 gtgagtctgc cgttctcgtc cttatatgta tttgtgatga tcaaaaaatg tctcataagc
     2941 ttccccacat ccggcagacg cccggtcatt cacagacgtc cggttacttg tctaactcgc
     3001 cgtgcgctgc atccacgacc gccttaatgt cgactgccgc atccacgccg cccgtctcat
     3061 ccttccggat gtacaccaga tactcgatgt ttccttccgg tcccttgatg ggcgaatatt
     3121 ccagattctt tacaaaaaat ccgttctgtg acgcgaattc aatgatccgc tctaccactt
     3181 cctcgtgaac agccttgtca cgcacaacac cctttttccc gactttttcc cgtccggctt
     3241 caaactgggg cttgatgaga cagaccatct cgccgccgtc tcttaagagc tcccgtgccg
     3301 ggattaaaac cttggtaagg gaaataaacg acacatccac cgacgcaaaa tccagcacgt
     3361 catcgatatc cgccggggtc acatagcgga tattggtctt ctccatgcag accacgcgcg
     3421 gatcctggcg cagcttccat gcgaactgac catagccgac gtccacggaa tacaccttga
     3481 cggctccgtt ttgcagcatg cagtcggtaa atccaccggt ggaagctccg atatccatgc
     3541 agaccttacc ctccagcgtg attccgaaat gcgtcatggc tttttcaagc tttaatccgc
     3601 cgcggctcac gtatttgagc gtattcccct ttacggtgat ctcggcctcc gtgtcaaatg
     3661 tagcccccgc cttatcttcc cgctggttct ccacaaagac attgccttcc ataatcatgg
     3721 tctttgcctt ctccctcgac ggagccagtc ctctgtttac aagcaaaaca tctaatcgtt
     3781 ctttcaaaag tcattccttt ccgcaccgct ttcctgtgcg tgcagttctt catatgcctc
     3841 tatggtgcgc tccgtgatcg tctccgcatc gattccgact tccctgcgca gaatctccac
     3901 attgccatgt tccacgtact catccggaat cgcgatggca agcacccgca cagagagatt
     3961 gtgctcctca taaaaacgca tgacctgttc gcccatgccg ccgctgatca cattctcctc
     4021 catcgtaacg ataaggtcgt gatgccttgc tgcggacagg agcgcatcct catccagcgg
     4081 ctttgcaaac cgcgcattga ttaagctgct ctcatagccg agcgccttca gccgctcatg
     4141 caccgcctcc gccgtcttca ccatcgcacc aagtgcaaac agcgcaatct gacccgaccg
     4201 gtagatccac tcactcttgc cgcaggcgat cggcgcccgg tactctctta atccgtcata
     4261 cgcctctcct ctcggatatc ttatcgcaat cggcgcggaa aaatcaactg catatttaat
     4321 catatccgac agttcccatt tattcttcgg cgccataatg gtcatattcg gtatcgttga
     4381 gagataggag atgtcaaaaa ttccctggtg ggtctccccg tcgctgccca caagtcctgc
     4441 acggtcgatc gcaaatacca ccggcaggtt ctgaatacag acgtcgtgga ggatctggtc
     4501 gtaggcgcgc tgtaaaaaag atgaatacac ggcaaagatc ggctttaagc ctcccgccgc
     4561 gagccccgcc gcaaatgtca ccgcatgctg ctccgcaatg cccacatcaa aaaagcgctc
     4621 cggaaacata ttgtggaaac gttttaatcc cgttccgtcg gtcatcgccg ccgtgattgc
     4681 caccacgcgc tcatcacggt cgccaagctt gcgcatcacc gtggaaaaaa catcggtgta
     4741 atttgccttt gtccgcggat gcttcggaag tcccgtctcg atatcaaacg gctccgtccc
     4801 gtggaaccgc gccggatgcc gctcggcagg tgcatagcct gttcccttgt gcgtgatgac
     4861 atgcaccagc accggtccat ccacacgcga cgcttcctgc agcannnnnn nnnntaaata
     4921 atccccagtt cctcaaacag catccccgga atgaacagct gcttaattcc gctcttcgtg
     4981 cgccggatac gctccaccat cttatcgccg tagaccggaa tcttgttgag ggaattcatg
     5041 accccgagct ttaaatcccg gtaggcatcc gccgtgcgga acccagccag gtaggtggac
     5101 agtccgccga cattctccga gatggacata ttattgtcgt ttaggacaat gataaagttg
     5161 gagttaagct gtgccgcatt attaagcgcc tcaaacgcca tacctccggt cagcgcaccg
     5221 tctccgatca ccgacaccac cttgtaattc tccccggtaa tctctctcgc atgcgcgt
//
LOCUS       DBMDQJ010000070         5261 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6151_length_5261_cov_1.446899, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000070 DBMDQJ010000000
VERSION     DBMDQJ010000070.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6151_length_5261_cov_1.446899"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(158..1750)
                     /locus_tag="ACZO9R_02295"
     CDS             complement(158..1750)
                     /locus_tag="ACZO9R_02295"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR0911935.1"
                     /translation="METVRFDELDLYPQVLRAIADMGFEEATPIQSQAIPVVMSGVDV
                     IGQAQTGTGKTASFGIPVLHKVDPNSRKTQVIILSPTRELAIQVADEIRKLAKYMHGV
                     KVLPVYGGQEIGRQIKALKGGAQIIVGTPGRVMDHLRRKTIRCDAIHTVVLDEADEML
                     NMGFRDDIETILEYIPAEGRQTILFSATMPKPILDITRKYQHDAVNIKVVKKELTVPS
                     IEQYYYDVKRSDKVEVLTRLLDYYNPKLSLVFCNTKRMVDELAEELKGRGYFAEGLHG
                     DMKQSQRDRVMKGFRSGKTEILIATDVAARGIDVDDVEAVFNYDIPQDDEYYVHRIGR
                     TGRAGRTGRAFTFVKGKEVYKLKDIMRYCKTKIVAMPIPSTDDVAQIKAEKVMEEIGR
                     IIDEENLSDMIDMIERQVNASDYTAMDIAAAFLKQALGQVNLDNGSDADYDFDNTGAE
                     EGMVRLFINIGKKDRVKPGDILGAIAGESGMPGKLVGAIDMYDKYTFVEVPREYGREV
                     LEGMKNTKIKGRSVNMEPANQR"
     gene            complement(2063..3700)
                     /locus_tag="ACZO9R_02300"
     CDS             complement(2063..3700)
                     /locus_tag="ACZO9R_02300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR0911936.1"
                     /translation="MEFKEYSKAVMAWVDGVMQNRGADAEKTLKYCADIEQYAKQTGD
                     PKLLGFAYYYAGETYYVLNEGEQLLKTITRAITYLDQAEQWDMVARAYNILAIAFLNR
                     GNTPIALDYYLTGLGYCKKYKLANEENVINLNLGTLYLGNEQWNEAQRYFEKVSSDIK
                     AYPETPNYYGLMSCVAVCLGRCFMQREQNERVQAQMDYLDQVCWEHMQKIERLSALIF
                     KAEYYHHVGRITLREECIEQIRGLVDMDMAVMDIFDDVYGLCRLLLEIGQEDVFWDIV
                     AVLEKLTKNANIANLQRKIVSLKILCYRRKQDEAAYLEEAGRFYELTEALDRENHYMI
                     ANMLSVRRSLEHANEKRREMEKANERLLEKSETDPLTRLANRFRLNDYLERAFERTRD
                     DHRAFAVEILDIDYFKEYNDNYGHQAGDACIVAIADELRKMQSRDTFCARYGGDEFII
                     IYEYKTEDEVFSMADSLRERILDRRIEHVCSKALPVVTISQGICFDVAPEGSRSWDFL
                     HAADMMLYEVKKRSRNNISLGHLDAKEARYGEERKLL"
     gene            complement(3783..4565)
                     /locus_tag="ACZO9R_02305"
     CDS             complement(3783..4565)
                     /locus_tag="ACZO9R_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR0911937.1"
                     /translation="MRKSRFWAFLGRKQYVIAGAIVIAAAVATTVIYSDHEQQQREQL
                     EEELAQENTPEEAGLVAATESTEQTAQASAVIPPEQTAETEKLEQKAAETTESTEVAQ
                     VEKEAENETAETGAEANALHFDPEDGMLWPMEGNVILNYSMDSTIYFATLDQYKYNPA
                     VIIAGEVNNKVYSVAKGKVIGLSNNEETGCTMTVDLGDGYRAIYGQLKEPNFAVGDYV
                     ESGHVLGYVAEPTKYYSVEGSNLYFALQKDGQPVDPVAFFQS"
     gene            complement(4640..>5261)
                     /locus_tag="ACZO9R_02310"
     CDS             complement(4640..>5261)
                     /locus_tag="ACZO9R_02310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR0911938.1"
                     /translation="AAFSEEELKQALGETYEKVMERLETCVEETSGQVLSWNGGYAYA
                     AYHAISAGSTRDMSRSAEAAVPYLPAVECPADLTAEGTLSVIYCEETDILKQCAEHFA
                     EADVSAVSEIQVTERDAAGYVQQVMLGEYVCTGEAFREAMGWNSACFTITQMGENVRI
                     VTRGLGHGYGLSQNEARAMAQEGTSYEEILQYFFPGTTLVTADQIK"
BASE COUNT         1101 a         1629 c         1102 g         1429 t
ORIGIN      
        1 aagaaatacg tgaattttgc ttcaaagaat ttcccgccgt ctgtttattc tggaactttt
       61 ttgttctctg gtcttctaat aatattactt atcaccccaa agaggtctat gcttattgcg
      121 gcaattccag attccgatag cgttccattt attaaatcta cctttgattt gccggttcca
      181 tattgacaga gcgccctttg atcttagtgt tcttcattcc ctcgaggact tctctgccat
      241 actctctcgg cacttccacg aaggtatact tgtcatacat atcgatcgcg ccgaccagct
      301 tgcctggcat gccggactcc ccggcgatcg ccccgagaat gtctcccggc ttgacgcggt
      361 cttttttacc gatgttgatg aagagacgca ccatcccttc ctccgcgccg gtattgtcga
      421 agtcgtagtc agcgtcgctg ccgttgtcga gattgacctg gccgagtgcc tgcttcaaaa
      481 atgccgctgc aatatccatt gcggtgtaat ccgaagcatt cacctggcgc tcgatcatgt
      541 cgatcatgtc cgacaggttc tcctcgtcga tgatcctgcc gatctcctcc atgaccttct
      601 ctgccttgat ctgtgccacg tcatccgttg acggaatcgg catcgccacg atcttggtct
      661 tgcagtagcg catgatatcc ttcagcttgt agacttcttt gcccttcacg aaggtaaaag
      721 cgcgtccggt ccgacctgca cgtcccgttc tgccgatccg gtgcacatag tattcgtcat
      781 cctgcggaat gtcgtagttg aaaacggcct ccacatcatc gacgtcgatt ccgcgcgccg
      841 ccacatcggt cgcaatgagg atctcggtct tgccgctgcg gaatcctttc atcacgcggt
      901 cacgctgcga ctgcttcata tctccgtgca gtccctctgc aaaatagccg cgtcccttta
      961 attcttccgc aagttcgtcc accatccgct tggtattgca gaataccagc gagagcttcg
     1021 gattgtagta atcgagaagc cgcgttaaga cttctacctt gtcgctgcgc ttgacatcgt
     1081 agtaatactg ctcaatgctc ggaaccgtca gctctttttt taccaccttg atgttgaccg
     1141 catcgtgctg atatttcctg gtgatatcga gaatcggctt cggcatcgtc gcggagaaaa
     1201 ggatcgtctg acgtccctcc gccggaatat actcaagaat cgtctcgatg tcatcgcgga
     1261 agcccatgtt cagcatctcg tccgcctcat cgagtaccac cgtgtggatc gcatcacagc
     1321 ggatcgtctt gcggcgcaga tgatccatca cgcgtcccgg cgttccgacg ataatctgcg
     1381 caccgccctt taacgccttg atctggcgtc cgatctcctg cccgccatac accggaagca
     1441 ccttgacccc gtgcatatac ttggcaagtt tgcggatctc gtccgccacc tggatggcaa
     1501 gctctctggt cggagagaga atgattacct gcgtctttct gctgttcggg tctaccttgt
     1561 gaagaaccgg aataccaaag gatgccgtct taccggtacc tgtctgtgcc tgtccgatca
     1621 catccacgcc cgacatcaca accggaatcg cctggctctg aatcggcgtt gcctcctcaa
     1681 atcccatatc cgcaatcgct cttaacacct gaggatataa atctaactca tcaaatctaa
     1741 ctgtttccat ctttttcctt tctgtcacgc cagagccaat tctctgacgc atgcccagca
     1801 ccggcagaaa gtgcccacct ctgccgactg cgcagtattt tgaatcatag cacggcatct
     1861 ctcttttgtc aagaaatgcg caaatctccg gacttctgtc tatgtaaccc aaatccgctc
     1921 gcggctttgg gcaatactcc tatgaaaagc aatcgacaga tgcgtacttt acttgatctg
     1981 tactttttca tactgctgcg cttcggtaaa agtaaaaggc tcgattgcgg attttccgtt
     2041 acagtatatt ctatgccgga atctataaaa gtttcctctc ttctccgtat ctcgcctctt
     2101 ttgcatccag atgtccgagc gaaatattgt tgcggctgcg ctttttcacc tcgtagagca
     2161 tcatatccgc cgcatgcagg aaatcccagc tccggcttcc ctccggagcc acgtcaaaac
     2221 agattccctg cgagattgtg acaaccggaa gcgccttcga gcacacatgc tcgatccgcc
     2281 tgtcaagaat acgctcccgc agactgtccg ccatggaaaa aacctcgtcc tccgtcttgt
     2341 actcgtaaat aatgataaac tcatccccgc cgtaccgcgc gcagaaggtg tcgcggctct
     2401 gcattttccg cagctcatcc gcaatcgcca cgatgcaggc atctcccgcc tgatggccgt
     2461 agttatcatt gtattccttg aagtagtcga tgtcgagaat ctctaccgca aatgctctat
     2521 ggtcatcccg cgtccgctca aacgcacgct ccagataatc attcaaccgg aaccggttcg
     2581 ccagccgcgt cagcggatcc gtctcggact tctccagcag acgctcattc gctttctcca
     2641 tctcccgccg cttctcattc gcatgttcta atgatctgcg cacggagagc atgttggcaa
     2701 tcatataatg gttctcccga tccagcgcct cggtcagctc atagaaccgg cccgcctcct
     2761 caagataagc cgcctcgtcc tgtttcctgc ggtagcagag gatttttaag gaaactattt
     2821 tccgctgtag atttgcaata ttggcatttt tcgtcaattt ttccaatacc gccacgatgt
     2881 cccagaagac atcttcctgg ccgatctcca gcaacagtct gcacagcccg tacacgtcat
     2941 caaagatatc cataaccgcc atgtccatat ccacaagtcc gcggatctgc tcaatacatt
     3001 cctcacggag tgtgattctt cccacgtggt gataatattc cgccttaaaa atcagcgccg
     3061 agagccgctc gatcttctgc atgtgctccc agcagacctg atccagataa tccatctgtg
     3121 cctgcacgcg ttcattctgt tcccgctgca taaagcaccg ccccagacag accgctacac
     3181 agctcatcag cccgtaataa ttcggggtct ccggatatgc cttgatatcg gaggagactt
     3241 tttcaaaata tctctgcgcc tcattccact gttcgttgcc cagatagagg gttccgagat
     3301 tcaggttgat gacgttttct tcgttcgcca gcttatattt tttacaataa ccgagtccgg
     3361 tcagatagta atccagcgca atcggcgtat ttccacggtt caaaaaggca atcgcaagga
     3421 tattataagc cctggcaacc atgtcccact gctccgcctg atccagatag gtgatggctc
     3481 tggtaatggt cttaagaagc tgctcgccct cattgagcac atagtaggtc tcgcccgcat
     3541 aatagtaggc aaaccccaga agctttggat cccccgtctg ttttgcatat tgttcgatat
     3601 ccgcacagta tttcaatgtt ttctcggcat cggcgccgcg gttctgcatc actccgtcca
     3661 cccacgccat cacagcttta ctgtattcct tgaattccat gtaccgattc ctcccttatc
     3721 tcttgtttca ttataatgaa gaaatccaac atattcaaga accggtgtct tccttttaca
     3781 aactagctct gaaaaaatgc taccggatcg acaggctgtc cgtctttttg cagcgcaaaa
     3841 tacagattgc tgccctccac cgaataatat ttcgtcggct ctgccacgta gcccagcaca
     3901 tggccggatt ccacataatc acccaccgca aagttcggct cctttaactg tccgtagatc
     3961 gcgcggtagc cgtcacccag atcgaccgtc atcgtacagc cggtctcctc gttgttggag
     4021 agaccgatga cttttccctt tgcgacggaa tataccttat tgttgacctc tcccgcgata
     4081 atcaccgccg gattgtattt gtactggtcc agcgtcgcaa agtatatggt agaatccatg
     4141 ctgtaattta agatcacatt tccctccatc ggccagagca tgccgtcctc cgggtcaaaa
     4201 tggagcgcat tggcctctgc gcccgtctcc gcagtctcat tctccgcttc tttttccacc
     4261 tgcgccactt ccgtactctc tgtcgtctcc gctgctttct gctccagctt ttccgtctcc
     4321 gcggtctgct ccggcggaat gactgcggat gcctgtgctg tctgttccgt actttccgtc
     4381 gcagcgacaa gtccggcttc ctccggtgtg ttttcctgtg ccagttcttc ctccagctgt
     4441 tcgcgctgct gttgttcatg atcgctgtaa attaccgtgg tcgcaactgc tgccgcaatc
     4501 acaatcgccc ccgcaatcac atattgttta cgcccgagga atgcccaaaa tctgcttttt
     4561 ctcatttttt tcaccatcca tattatttat ttttattcct attgttcccc ctgcacggat
     4621 ggtttatgca gagcgtcgcc tattttatct gatccgctgt cacaagcgtt gttcccggaa
     4681 aaaaatattg taaaatctcc tcgtaagagg ttccttcctg cgccatcgcg cgcgcctcat
     4741 tctggctcag tccgtacccg tgtcccagtc cccttgtcac gatccgcaca ttttccccca
     4801 tctgcgtgat cgtaaaacac gcggaattcc agcccatcgc ctcacggaac gcttccccgg
     4861 tgcagacata ctcccccagc atgacctgct gtacatagcc cgccgcatcc cgctctgtaa
     4921 cctggatctc agacaccgcg gacacgtctg cctccgcaaa atgctccgcg cactgcttta
     4981 aaatgtccgt ttcctcacaa taaataaccg agagcgtacc ctccgccgtc agatccgccg
     5041 gacactccac cgcaggaaga tacggcaccg ccgcttccgc cgaccggctc atatctctcg
     5101 tacttcctgc actgatcgca tgatatgcag catacgcata gccgccgttc caggagagca
     5161 cctgtccgct cgtctcctcc acgcacgttt caagccgttc catgactttt tcatatgttt
     5221 cccccagcgc ctgctttaat tcttcctccg aaaaagcagc c
//
LOCUS       DBMDQJ010000071         5226 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6201_length_5226_cov_1.396098, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000071 DBMDQJ010000000
VERSION     DBMDQJ010000071.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6201_length_5226_cov_1.396098"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="ACZO9R_02315"
     CDS             <1..273
                     /locus_tag="ACZO9R_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911939.1"
                     /translation="SIAESMAETLAKPMAETLAKPMAETLAKPMAESLAASKVAQSIL
                     SLAAELGTIPAEEQQRIAGEQDDETLEKWLKLAARSTTVEEFLSGM"
     gene            443..4105
                     /locus_tag="ACZO9R_02320"
     CDS             443..4105
                     /locus_tag="ACZO9R_02320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR0911940.1"
                     /translation="MSFAHLHVHTEYSLLDGSNKIKEYVKRIKELGMTAGAITDHGVM
                     FGVIDFYKEAKAAGINPVLGCEVYVAPNSRFDREMSHGEDRYYHLVLLAENNQGYQNL
                     MKIVSKGFVEGYYYKPRVDMELLEQYHEGLIALSACLAGEVQRYLVRGLYDEAKKTAQ
                     RYEACFGKGNYFLELQDHGIPDQKTVNAGLMRLSQELGIELVATNDVHYTYAEDWEAH
                     DILLCLQTGKKLSDENRMRYEGGQYYVKSEEEMRALFPYAAQAIENTQKIADRCHVEI
                     EFGVTKLPHFTVPDGYDSWTYLNKLCHEGLIQRYPDRHDELLPQLDYELSVIQKMGYV
                     DYFLIVWDFINYARTHGIPVGPGRGSAAGSLVSYTTGITNIDPIRYNLLFERFLNPER
                     VTMPDIDIDFCYERRSEVIDYVIGKYGKDCVTQIVTFGTLAARGVIRDVGRVMDLPYN
                     FCDTIAKNIPNELNITIDKALTMNPELRTMYETDDTVHTLIDMAKRLEGLPRHTSMHA
                     AGVVISQKAMDEYVPLSRASDGTITTQFVMTTIEELGLLKMDFLGLRTLTVIKDAVAL
                     VEKNHGVRIDVDHIDYNDNSDPDAIQIDYGDKKVLDAIGTGKCEGVFQLESAGMKNFM
                     KELKPQSLEDVIAGISLYRPGPMDFIPKYIKGKNEPESITYTCKELEPILEPTYGCIV
                     YQEQVMQIVQNLAGYSMGQADNIRRAMSKKKQYVIDAERHNFVYGNEEQGIKGCIANG
                     ISEKAANEIYDSMVDFAKYAFNKSHAAAYAVVAYQTAYLKYYYPVEFMAALMTSVIDN
                     PRKVAEYIYTCRQMGIKVLAPDINEGEGRFSATKDGIRYGLYAIKSIGRSVVDAIIAE
                     RNQNGPYRTLQNFIERVADSEVNKRTVENLIKSGACDTLDGNRQQMMMVYNALMDQQV
                     QTKKKSLAGQMSLFDLVSEEEKKAYEIQYPNVGEYSKEIKLGFEKEVIGIYLTGHPLE
                     EYEEKWRRIISAVTSDFLLDEESGEVKLRDNQKVIIGGMITEKNIKYTKNNKTMAFLL
                     LEDLFGTVEVIVFPGEYERYHTLLNEDEKVFIRGHANVEEDKNAKIVCEQICTFEQAE
                     NGMPQEQRTYRQGGGYRRGAGGTAGSSPASAGDGGSSRGGAAAQVPARRGDELWLQFE
                     TKEAFAEKEKELYAMLHDSDGKDTVVIYISSVKAMKRLPENYSICIEPEIVNNLTKFL
                     GENNVKVVKKSIEKKP"
     gene            4161..5135
                     /gene="pfkA"
                     /locus_tag="ACZO9R_02325"
     CDS             4161..5135
                     /gene="pfkA"
                     /locus_tag="ACZO9R_02325"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR0911941.1"
                     /translation="MAKEINTIGVLTSGGDAPGMNAAIRAVVREAIAKGKKVKGIKRG
                     YAGLLQEEIVDMEAKDVSDIIQRGGTILQTARCMEFKTPEGQQKAAEICRKHGIDGII
                     VIGGDGSFRGAQKLAALGINTIGLPGTIDLDIACTEYTIGFDTAVNTAMEAIDKVRDT
                     STSHERCSIIEVMGRGAGYIALWCGIANGAEDILLPEKYDYDEQKIVNHIIENRKRGK
                     QHHIIVNAEGIGHSASMAKRIEAATGVETRATILGHMQRGGSPTCKDRVYASTMGALA
                     VDLLCEGKSNRVVGYRHGDFVDDDIDEALAMQKSIPEYQYQISKNLSM"
BASE COUNT         1426 a         1107 c         1545 g         1148 t
ORIGIN      
        1 tcgatagcgg aatcaatggc ggaaacgctt gcgaagccga tggcagagac gcttgcgaag
       61 ccgatggcag agacgcttgc gaagccgatg gcggagtcac ttgcagcgtc aaaagtagca
      121 cagagtatat tgtcattagc agccgagctt ggaactattc cggcagaaga gcagcagcgc
      181 attgcagggg aacaggatga tgagacactg gaaaagtggc tgaaacttgc tgcacgctca
      241 acaactgtgg aagagttcct gtctggaatg tagagacgag tctgtctcgt tcttaagcgg
      301 cgagtctgcc gctgtcatca accaaacaat ttcgggacgt gtctgtctat ctatttctgc
      361 tatggaaagc acgggaatga tccgtgaaaa gatttgaata attccgttgt tcataatata
      421 atgaaaaaac ggaggattta ttatgtcatt tgcacattta cacgtccata cagagtacag
      481 tctgctggac ggttccaata aaatcaagga atatgtaaaa cgaatcaaag aactcggcat
      541 gacagcggga gccatcacgg atcacggtgt aatgtttggc gtgattgatt tttataaaga
      601 agcgaaggcg gcagggatca atcctgtttt gggctgcgag gtctatgtgg cgccgaattc
      661 cagatttgac cgcgagatgt cccatgggga ggaccgctac tatcatctgg tgctgctggc
      721 ggagaataac caggggtatc agaatctcat gaagatcgtg tcgaaaggat ttgtggaggg
      781 atactactac aaaccgcgtg tcgatatgga acttttagaa cagtatcatg aggggctgat
      841 tgcgttgagc gcctgtctgg caggcgaggt gcagcgctac ctggtcagag gtctttatga
      901 cgaggcgaag aagacggcgc agcgctatga ggcatgcttc ggcaagggca attattttct
      961 agaactccaa gatcacggaa tcccggatca gaagacggtg aatgccggcc tgatgcggct
     1021 tagccaggag cttggcattg agcttgtcgc caccaacgac gtgcactata cctatgcgga
     1081 ggactgggag gcgcatgata tcctgctgtg tctccagaca gggaaaaagc tgtccgatga
     1141 gaaccgtatg cgctatgagg gcggacagta ttatgtcaaa tccgaggagg agatgcgcgc
     1201 acttttccct tacgccgcgc aggcgattga gaatacccag aagattgccg accggtgcca
     1261 tgtggagatt gaattcggtg tgacgaagct gccgcacttt acggtgccgg acgggtatga
     1321 ttcctggacg tacctgaaca aattgtgtca tgagggactg atccagcgtt atccggaccg
     1381 ccatgacgag ctgctgccgc agcttgatta tgagctttcc gttatccaga agatgggata
     1441 tgtcgattac ttcctgatcg tgtgggattt tatcaattat gccagaacgc acggcattcc
     1501 ggtcggaccg gggcgtggaa gcgcggcggg aagtcttgtc tcctacacga ccggtatcac
     1561 caatattgat ccgatccggt ataatcttct gtttgagcgt tttttaaatc cggagcgtgt
     1621 cacgatgccg gatattgata ttgatttctg ctatgagaga cgaagtgagg tcattgacta
     1681 tgtaatcggg aaatacggca aggactgcgt gacgcagatc gttacattcg gtacgctggc
     1741 tgcgcgcgga gtcatccgcg atgtggggcg tgtgatggat ctgccgtaca atttctgtga
     1801 tacgattgcg aagaacattc caaacgaact gaacatcacc atcgataagg cgcttaccat
     1861 gaatccggag ctgcgcacca tgtacgagac ggatgacacg gtgcatacac tcatcgatat
     1921 ggcaaaacgt ctggaaggac tgccgcgcca tacgtccatg catgcggcgg gtgtcgtgat
     1981 ttcacagaag gcgatggatg agtatgtacc gctttccaga gcttcagacg gcacgattac
     2041 cacgcagttc gtcatgacaa cgatcgagga actcggtctg ttaaaaatgg acttcctcgg
     2101 acttcggacg cttaccgtaa tcaaagatgc cgttgccctt gtggagaaga atcacggtgt
     2161 gcggatcgac gtcgatcaca tcgattataa tgacaacagc gatccggatg cgatccagat
     2221 tgattacggc gacaagaagg tgctggatgc gattggaacc ggaaaatgcg agggcgtctt
     2281 ccagcttgaa agtgccggga tgaagaactt catgaaggag ttaaaaccgc agagtctgga
     2341 ggatgttatc gccggaatct ctctgtaccg tccgggaccg atggatttca ttccaaagta
     2401 cattaaggga aaaaacgagc cggagagcat cacatacacc tgcaaggaac tggaaccgat
     2461 cctggagccg acatatggct gtattgtcta ccaggagcag gtcatgcaga tcgtgcagaa
     2521 tcttgccggc tattccatgg gacaggcgga taacatccgc cgtgcgatga gtaagaaaaa
     2581 gcagtatgtc attgatgcgg agcgccacaa tttcgtttac ggaaatgagg agcaggggat
     2641 caaaggctgt attgcaaacg gtatcagtga gaaggcggcg aatgagatct acgattccat
     2701 ggtggatttt gcgaagtacg cattcaacaa atcccatgcg gcggcatacg cggtggtcgc
     2761 atatcagacg gcgtatctga aatattatta tccggtggaa tttatggcgg cgctgatgac
     2821 ctccgtgatt gataacccgc ggaaggtggc ggagtatatc tatacctgca gacagatggg
     2881 aatcaaagtg ctggccccgg atatcaatga gggcgaggga cgtttttcgg cgacgaaaga
     2941 cggcatccgc tacggactgt atgcgatcaa gagtatcggg cgttcggtgg tggatgcgat
     3001 catagcggag cggaatcaga acggtcccta ccgtaccctg cagaatttta ttgagcgggt
     3061 ggcggactct gaggtaaaca agcgcaccgt cgaaaatctc atcaaatccg gtgcctgcga
     3121 taccttagac ggtaacagac agcagatgat gatggtgtac aacgctctga tggatcagca
     3181 ggtgcagacg aaaaagaagt cgctggcggg acagatgagc ctgtttgatc tggtgtcgga
     3241 ggaggagaaa aaagcctatg agattcagta tcccaacgtt ggagagtaca gcaaggagat
     3301 caagcttggc tttgaaaagg aagtcatcgg tatctatctg accggtcatc cgctggagga
     3361 atacgaggag aaatggcgca gaatcatatc ggcagtgacg tcggatttcc tgctggacga
     3421 ggagagcggc gaggtcaagc tgcgggataa ccagaaggtt atcatcggcg gaatgattac
     3481 ggagaagaac atcaaatata cgaagaataa taagacgatg gcatttctat tgctcgagga
     3541 tctgttcggt acggtggagg tgatcgtatt ccctggcgag tatgagcgct atcatacgct
     3601 gctgaatgag gatgagaagg tatttatccg cggacatgcc aacgtggagg aggataaaaa
     3661 cgccaagatc gtctgcgagc agatctgtac gtttgaacag gcagagaacg gaatgccaca
     3721 ggaacagcgc acttaccggc agggcggagg ataccggcgc ggtgcaggcg ggactgcggg
     3781 cagcagtccg gcatccgcgg gagacggcgg atcaagccgt ggcggtgcag ccgcgcaggt
     3841 tcccgcgcgg cgcggcgatg aattatggct tcagtttgag accaaggaag cgtttgcgga
     3901 aaaggaaaaa gaactctatg ccatgctcca tgattcggat gggaaagaca cggtggtgat
     3961 ctatatttca tcggtcaagg cgatgaagcg gctgcccgaa aactacagta tctgcatcga
     4021 accggagatt gtgaacaatt taactaagtt tttgggggaa aataacgtaa aagttgtcaa
     4081 aaagagcatt gaaaagaagc cataaagaga ttaaaataag atagattaaa tgggtatatt
     4141 atatgaggag ggaacgataa atggcgaaag agataaacac cattggtgta ctcacgagcg
     4201 gcggcgatgc cccgggtatg aacgcagcaa ttcgtgcagt ggttagggaa gccattgcaa
     4261 agggtaagaa agtcaaggga atcaagagag gttatgcagg acttctccag gaagagatcg
     4321 tagatatgga agcaaaagac gtttccgata tcatccagcg cggcggtact attttacaga
     4381 cagcacgctg catggagttt aagacaccgg aaggtcagca gaaagcagca gagatctgca
     4441 ggaagcacgg aatcgacggt attatcgtta tcggcggtga cggttctttc agaggtgcgc
     4501 agaagctggc ggcactcggc atcaacacga tcggtcttcc gggaacgatt gaccttgata
     4561 ttgcatgtac agagtatacc atcggttttg atacagcggt aaacaccgca atggaagcga
     4621 tcgacaaggt acgtgatacc tctacttctc atgagcgttg cagcatcatc gaggttatgg
     4681 gacgcggcgc gggctatatt gcattatggt gcggtattgc gaacggcgca gaggatatcc
     4741 tgcttccgga gaaatatgat tacgatgagc agaagattgt aaaccatatc atcgagaacc
     4801 gtaagcgcgg caaacagcat cacattattg tcaatgcgga ggggatcgga cattctgcaa
     4861 gcatggcaaa gcgtatcgag gctgcaaccg gcgttgagac gcgtgcgacg attctggggc
     4921 acatgcagcg tggcggaagc ccgacctgta aggatcgtgt ctatgcatcc acgatgggtg
     4981 cgctggcagt ggatctgctc tgcgagggca agagcaaccg tgtcgttggt tatcgtcacg
     5041 gtgatttcgt ggatgacgac atcgatgagg cacttgcaat gcagaaatcc attccggagt
     5101 atcagtatca gattagcaag aacctttcaa tgtaagatac ataacagtat ttggaacgat
     5161 cataagactc catgggcggg aactcatgga gttttttccg cttagggaac aaaaaggagc
     5221 agtcat
//
LOCUS       DBMDQJ010000072         5205 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6228_length_5205_cov_1.695925, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000072 DBMDQJ010000000
VERSION     DBMDQJ010000072.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5205)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5205)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5205
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6228_length_5205_cov_1.695925"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /gene="argH"
                     /locus_tag="ACZO9R_02330"
     CDS             complement(<1..719)
                     /gene="argH"
                     /locus_tag="ACZO9R_02330"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR0911942.1"
                     /translation="MAQLWGGRFTKQTDKLVYNFNASISFDQKFYKQDIEGSIAHVRM
                     LGKQGILTGEEMQAIVACLKEILADVEAGRLVITDEYEDIHSFVEANLIDRLGDTGKK
                     LHTGRSRNDQVALDMRLYTREELLHVDALLRELLGTILHIMEQNTETIMPGFTHLQKA
                     QPITLAHHMGAYFEMFKRDRLRLHDIYERMNYCPLGSGALAGTTYPLDRAYTAELLGF
                     YGPTLNSMDGVSDRDYLIEFLS"
     gene            complement(803..>1074)
                     /locus_tag="ACZO9R_02335"
     CDS             complement(803..>1074)
                     /locus_tag="ACZO9R_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR0911943.1"
                     /translation="KSLGMDYFTTTLSISPLKNAEKLNEIGDALAEEYGVAYLNSDFK
                     KKNGYKRSVELSEQYGMYRQYYCGCVFSKRERDAQIAAKAAAQGI"
     assembly_gap    1075..1084
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1085..1430)
                     /locus_tag="ACZO9R_02340"
     CDS             complement(<1085..1430)
                     /locus_tag="ACZO9R_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HIR0911944.1"
                     /translation="MRAQKNYQKMLEATIAGLKEQNRVPTLLLHSCCAPCSSYCLEYL
                     SQYFHITVDYYNPNIYPEEEYHMRVQEQQRLIRELPAKYPIAFREGAYDKERFYEMAR
                     GMEEEKEGGERCF"
     gene            complement(1427..2617)
                     /locus_tag="ACZO9R_02345"
     CDS             complement(1427..2617)
                     /locus_tag="ACZO9R_02345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0911945.1"
                     /translation="MMQERKQKGKILVFSSREICYLSSNFFANQIGAAFEELGYEVTV
                     CELSKEDDLDAKLARYIGQPYRLILDFNSLLPRMVLDDGTPYVDRLAGPFFDYILDHP
                     LFHYQGLSSGVKNLHAIVLDEAQQKYVEKYYEKVASVHMLPLGATRAVYEGTKEPECR
                     ILFPGTYDRPDAVYQIVENAPEPLGSMMKDLIERRLADPTLPMEEAFSQQLKEEKLEL
                     PAGQFALFMNSMYAVDAYVRDYFRKAVLDALLAEKLPVTVFGEGWEKYSCGDEHSLRR
                     EPAVPFALSFERIAKAHVLLNVSPIFNRGMHDRVPAGMANHAVVLTDENPYLRRTFTD
                     GKELLFYSLEKPDTLFEQAERALTDVRLREKIAASAYETFEREYTWKHRAEQILAFAE
                     EVEA"
     gene            complement(2614..3138)
                     /locus_tag="ACZO9R_02350"
     CDS             complement(2614..3138)
                     /locus_tag="ACZO9R_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0911946.1"
                     /translation="MLEKLFLEMIAYYSGDPKRIQHFTKVHSYAKLIGEREQLSPEEL
                     YILEAAAYTHDIGIKPAEEKYGSSEGKLQEQEGPAVARGMLMRLGFAENVIERVCYLI
                     GHHHTYTGIEGRDYQILVEADFLVNLYEDGRPDGDGGFMPDKAAVETAYQRIFATETG
                     KWICRSMFALGGVS"
     gene            complement(3200..3505)
                     /locus_tag="ACZO9R_02355"
     CDS             complement(3200..3505)
                     /locus_tag="ACZO9R_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911947.1"
                     /translation="MGEIANRDNKKMLILFAEVVAALATILIAVLAMKIPVVPVCVIV
                     LIEVAIAACLQEVPVWVHAVVAAVQLVAGVLCGRAVFCLLCILIYVFAILTLREARA"
     gene            3691..4866
                     /locus_tag="ACZO9R_02360"
     CDS             3691..4866
                     /locus_tag="ACZO9R_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR0911948.1"
                     /translation="MNDTKWVSMWGNAMSIAEHHPEGYAKDLTLRYPVYAPFSGSALR
                     FTLDNYCGKEPVTIHRATVAVCDSDLTGKVPLSCPLAAGTIRDITFDGSHSVTLAAGA
                     SAASDALSFPVSAGQTLCVSLYFDGYTAMQSAVLITGPLSRGYFSYGDQTHSEALPLA
                     TSKATNWFYFLSNIDILTKEDCHAIICYGDSITAQAWPDYLTLRLIEEGHNHTSVIRR
                     AASGTRILRQYDCITYDSYGLKGTNRFAHEIPTAGADTVIIQQGINDIIHPVGTDVNP
                     FRPMSDLPTAAELIDGLKWYIEEARKLHDSVYLGTLLPIYGWRTYAPFREELRGAVND
                     WIRSNHESDGCIDFDQALRAADDPRSFAEGYDSGDHLHPSNPAYKKMADTVPATLLR"
     gene            complement(4867..>5205)
                     /locus_tag="ACZO9R_02365"
     CDS             complement(4867..>5205)
                     /locus_tag="ACZO9R_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR0911949.1"
                     /translation="EETILVKPAPVIVLEGILIFAEPSLCDLMDIKVFVDTDADVRIL
                     RRIIRDVKERGRSLDSVITQYLTTVKPMHEQFVEPSKRRADIIIPEGGENIVALDMLI
                     ERVRKHLKNK"
BASE COUNT         1220 a         1570 c         1125 g         1280 t
ORIGIN      
        1 gacaaaaatt cgatcagata atcgcggtca gacacgccgt ccatgctgtt taaagtcggt
       61 ccgtaaaaac cgagcagctc tgccgtgtat gcacggtcaa gcggatacgt ggtgcctgcg
      121 agcgctccgg aaccaagcgg acagtaattc atccgctcgt agatgtcatg caggcgcagg
      181 cggtctctct taaacatctc aaaataagcc cccatgtgat gcgcgagcgt gatcggctga
      241 gccttctgca ggtgggtgaa acccggcatg atcgtctccg tattctgctc catgatatgc
      301 aatatcgtgc cgagcagttc ccttaagagc gcatccacgt ggagcagttc ctctctggtg
      361 tagagacgca tatcgagcgc cacctggtcg ttgcggctgc gcccggtatg cagcttcttt
      421 ccggtgtctc ccagacggtc gatcagattc gcctccacaa agctgtgaat atcctcatat
      481 tcatccgtta taacaagacg tccggcttcc acatccgcaa ggatttcctt taggcatgcg
      541 acgatcgcct gcatctcctc cccggttaag attccctgtt tcccgagcat ccgcacatgt
      601 gcaatacttc cttcaatgtc ctgtttatag aatttctggt caaacgaaat agacgcatta
      661 aaattataga ccagcttgtc ggtctgtttg gtaaaacgtc ctccccataa ctgagccatt
      721 ttaaaaacct cgctttcact acatttccta tcattctaca tgaagttcgc gcttcccgca
      781 agccctttca tgctttaccg catcaaattc cctgtgccgc cgcttttgca gcaatctggg
      841 catcgcgctc ccgcttggaa aaaacgcacc cgcaataata ctgccggtac attccgtatt
      901 gttcggaaag ctctaccgag cgcttgtagc catttttctt tttgaagtca gaattcaaat
      961 atgccacgcc gtattcctcc gccagcgcat ccccgatctc atttaatttt tccgcattct
     1021 tgagcggact gatggacagc gtcgtggtaa agtaatccat gccaaggctt tttgnnnnnn
     1081 nnnnggaagc agcgttcgcc gccctccttt tcctcctcca ttccgcgcgc catctcataa
     1141 aaacgctcct tatcataggc gccctcccgg aatgcgatcg gatattttgc cggaagttcc
     1201 cgaatcagcc gctgctgctc ctgcacccgc atatgatact cttcttccgg ataaatattg
     1261 gggttgtaat aatccacggt gatatgaaaa tactgcgaca gatactcgag gcagtacgag
     1321 ctgcacggcg cgcagcagct gtgcagcagc agcgttggca cacggttctg ctcctttaat
     1381 ccagcgatcg tcgcctccag cattttctga taattcttct gtgctctcat gcttccacct
     1441 cctctgcaaa tgcaaggatc tgctccgcac ggtgcttcca tgtatattcc cgctcaaagg
     1501 tctcataggc agaagccgca atcttctcac gaagtcttac gtctgtaagt gctctctccg
     1561 cctgctcaaa cagcgtgtcc ggtttttcca gcgaataaaa aagcagctcc ttcccgtctg
     1621 taaacgttct tcttaaatac ggattttcat cggttaacac caccgcatga ttcgccattc
     1681 cggcaggaac ccggtcgtgc attccccggt taaaaatcgg tgagacattt aaaagcacat
     1741 gggctttggc aatccgctca aaggagagcg caaacggcac cgccggctcg cgccgcaggc
     1801 tgtgctcgtc cccgcaggaa tatttttccc agccttcccc gaatacggta accggcagtt
     1861 tctccgcgag cagcgcgtcc aacaccgcct tgcggaaata atcccgcaca taggcatcca
     1921 ccgcatacat gctgttcata aacagcgcaa actgtccggc aggaagttcc agtttctcct
     1981 ccttaagctg ctgtgaaaat gcttcctcca tcggcagtgt gggatcggca agcctccgct
     2041 caatcagatc tttcatcatg ctcccaagcg gctcgggagc attctctaca atctgataaa
     2101 ccgcgtccgg tctgtcatac gttcccggaa ataaaatccg gcactccggc tcttttgtgc
     2161 cctcgtagac tgctcttgtc gccccgagcg gcagcatgtg aacgctcgcc accttttcat
     2221 aatatttttc tacgtacttc tgctgtgcct cgtcgaggac aatcgcatgc aggtttttca
     2281 caccgcttga aagtccctga tagtgaaaca gcgggtgatc taaaatatag tcaaaaaacg
     2341 gtcccgcaag gcggtccaca tagggagtgc cgtcgtctaa caccatccgc ggcagaaggg
     2401 aattaaaatc cagaatcagg cggtacggct gtccgatata gcgcgcaagc ttcgcatcca
     2461 gatcatcctc ctttgagagt tcgcacaccg tcacctcata tcccagttcc tcaaacgccg
     2521 cgccgatctg atttgcgaaa aagttgctgg acagatagca gatttctctg gaactgaata
     2581 ccagaatttt tcccttctgc ttcctttcct gcatcatgac acacctccaa gtgcaaacat
     2641 gctccgacag atccattttc cggtctccgt cgcaaaaatc ctctgatatg ctgtctctac
     2701 agccgcttta tccggcataa acccgccgtc tccgtccggt ctgccgtcct cgtacaaatt
     2761 caccagaaaa tccgcctcca ccagaatctg atagtctctg ccctctatgc cggtgtaggt
     2821 gtggtgatgt ccgatcagat agcagacgcg ctcaatcaca ttctccgcaa agcccagccg
     2881 catcagcatc ccgcgtgcca ccgcagggcc ttcctgctcc tgcagctttc cctcactgct
     2941 tccatacttc tcctccgccg gcttgatgcc gatatcgtgc gtgtatgcag ctgcttccag
     3001 aatatagagt tcctccggcg aaagctgttc gcgctccccg atcagctttg catagctgtg
     3061 tacctttgta aaatgctgga tccgtttcgg gtctccgctg taatatgcga tcatttccaa
     3121 aaaaagtttc tctaacatct tttctccttt gacagaaaga agacctttac caagcatccg
     3181 cttcataaag gtcttatcat tacgctcttg cttcccgcag tgtcagtatc gcaaacacat
     3241 agatcaaaat acacaacagg cagaatacgg ctctgccgca aagtaccccg gcaaccagct
     3301 gtactgcggc gaccacggcg tgtacccaca ccggtacctc ctgcagacag gcagcaatcg
     3361 ccacctcgat caaaacgatc acacacaccg gcaccaccgg aatcttcatc gctaaaacgg
     3421 cgatcagaat cgtcgcaagc gccgcaacca cctctgcaaa caaaatcagc atcttcttat
     3481 tatcacgatt tgcaatctct cccatacaaa tgaactcctt tattgtcttt ctattctgtc
     3541 tgcaggtctt cacccgcatg cgctcattat agcaaggatc gaaaaaaaga acaagtccac
     3601 tggaaaaata ccttcacttc cgctataata acgcaggaat atactcttat attccttacc
     3661 agtaaataat aaagaaaaga gaacaaactt atgaatgata caaaatgggt ctccatgtgg
     3721 ggcaatgcca tgtccatcgc agaacatcat ccggaaggct acgcaaaaga tctgacgctg
     3781 cgctatccgg tctatgcacc tttttccgga agcgcactcc gcttcacgct cgacaactac
     3841 tgcggaaagg aacctgtcac catccaccgg gcaacggtgg cggtgtgtga ttccgatctc
     3901 accgggaaag ttcctctttc ctgtccgctt gctgcaggca ccatccgcga tattaccttt
     3961 gacggcagcc attccgtcac actggcagcg ggtgcatccg ccgcgagcga tgcactctcc
     4021 tttccggtat ctgccggtca gacgctctgc gtcagcctgt attttgacgg ctacactgcc
     4081 atgcagtccg ccgtgctgat cacaggtcca ttgtcccgcg gctatttttc ctacggcgac
     4141 cagacgcaca gcgaagcgct gccccttgcc acctccaagg cgacaaactg gttctatttt
     4201 cttagcaaca tcgatattct gacaaaggaa gattgtcatg ccattatctg ctacggagat
     4261 tccatcaccg cacaggcatg gccggactat ctgacgctgc gcctcatcga agaagggcac
     4321 aaccatacct cggtgatccg ccgtgcggca agcggtacgc gtattctgcg ccagtacgac
     4381 tgtatcacct acgattccta cggtcttaag ggaaccaacc gttttgccca cgaaattcca
     4441 accgccggtg cggataccgt catcatccag cagggcatca atgacatcat ccatccggtc
     4501 ggaaccgacg tcaatccgtt ccgtcccatg agtgatctgc cgactgccgc cgaactgatc
     4561 gacggtctga aatggtatat cgaggaggcc agaaagcttc acgacagcgt ctatctcgga
     4621 acccttctgc cgatttacgg ctggcgcacc tacgcaccgt tccgcgaaga gctgcgcggt
     4681 gccgtcaatg actggatccg ctccaaccac gagagcgacg gctgtatcga tttcgatcag
     4741 gcacttcgtg ccgccgatga tccgcgctct ttcgcggaag gatatgacag cggcgatcat
     4801 ctgcatccga gcaatcctgc atataaaaaa atggcagaca cagtgcctgc cactttattg
     4861 cgctaattat ttattcttta aatgtttgcg tacgcgttca atcagcatgt caagcgccac
     4921 gatattttct cccccttccg ggatgataat atctgcccgg cgcttgctcg gttctacaaa
     4981 ctgttcatgc atcggcttta ccgttgtcag atactgggta atcacgctgt ctaacgaacg
     5041 cccgcgttcc tttacatcac ggatgatccg ccgcagaatc cgcacatccg catctgtatc
     5101 cacaaatacc ttgatatcca taagatcaca gagactcggc tcagcaaaaa tgagaatgcc
     5161 ctccagcaca atcaccggtg caggttttac cagaatggtc tcctc
//
LOCUS       DBMDQJ010000073         5201 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6235_length_5201_cov_1.891961, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000073 DBMDQJ010000000
VERSION     DBMDQJ010000073.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6235_length_5201_cov_1.891961"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(92..715)
                     /locus_tag="ACZO9R_02370"
     CDS             complement(92..715)
                     /locus_tag="ACZO9R_02370"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR0911950.1"
                     /translation="MKKTVITIARSYGSGGRTLGKLLAEQLGINCYDREILRMASDAS
                     GINEALFGQADERLKRSPLFGILKKNPYKGGVIPPENSDFVSDDNLFNYQAKVIKELA
                     AQESCVIIGRCADYLLRDDPDVIKLYFCAPKKDCVARVMNQNGLSEKEAERRIEKIDK
                     YRAEYYRYYTGRDWNDARNYNFCLDTTSMSYEKLVEVVTNFIQIYQK"
     gene            complement(743..1453)
                     /locus_tag="ACZO9R_02375"
     CDS             complement(743..1453)
                     /locus_tag="ACZO9R_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911951.1"
                     /translation="MAMLEVKNLKVHYGVIQAIKGISFEVNEGEVIALIGANGAGKTT
                     TLQTITGMLKPSEGEILFEGQDIVKVPGHKIVSMGMAHVPEGRRVFAELSVYENLKLG
                     AYTRKDKNEIAESLAKVYKSFPRLEERKNQLAGTLSGGEQQMLAMGRALMSKPKIILM
                     DEPSMGLSPIFVEEIFHIIREISASGTTVLLVEQNAKKALAIANRAYVLETGNIVLSG
                     DAKEMMNNDSIKKAYLGE"
     gene            complement(1478..2236)
                     /locus_tag="ACZO9R_02380"
     CDS             complement(1478..2236)
                     /locus_tag="ACZO9R_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016295108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911952.1"
                     /translation="MAMLEVKNLGISFGGLRAVDDFHISIEKGQLYGLIGPNGAGKTT
                     VFNMLTGVYTPDEGTISLDGADITGKKTIDINKAGIARTFQNIRLFKDQSVLDNVKIG
                     LHNQMSYSTLTGILRLPKYRKVEAEMDEKAMELLKVFDLEREAGFLSSNLPYGKQRKL
                     EIARALATNPKLLLLDEPAAGMNPNETKELMETIHFVRDEFDMTILLIEHDMKLVSGI
                     CEKLTVLNFGQVLAEGATGDVLHDPQVIKAYLGE"
     gene            complement(2239..3315)
                     /locus_tag="ACZO9R_02385"
     CDS             complement(2239..3315)
                     /locus_tag="ACZO9R_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005350624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR0911953.1"
                     /translation="MAKLNLKTVNKKTKDQLITFVLVIAVYIVVQIMVAAGAISSLMQ
                     GLLVPMCTYSIVAIGLNLCVGYLGELSIGHAGFMCVGAFSSAFCTKMLEGVIGSQIVL
                     FIIALLVGTAMAAFFGFLIGIPVLRLRGDYLAIVTLAFGEIIKNIINVLYIAKDGSGF
                     HFAISNGNAIDLADDGVWLINGAKGIAKIPHLSSFTAGVILILISLFVVYNLVNSRSG
                     RAIMAIRDNRIAAESVGINITKFKLMAFTISAAIAGAGGVLYAHNLSTVTATPANFGY
                     NMSIMILVFVVLGGMGNFRGSIIAAVLLTLLPEVLRGLSDYRMLIYSIVLIVMMLFNW
                     APKCIEWRQKMAQKFRKNTKKEAV"
     gene            complement(3335..4216)
                     /locus_tag="ACZO9R_02390"
     CDS             complement(3335..4216)
                     /locus_tag="ACZO9R_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008725457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR0911954.1"
                     /translation="MNFLSYLINGISLGSVYAIIALGYTMVYGIAKMLNFAHGDVIMI
                     GCYVVFLAMSGQGISPLPAVVLSIIVCTVLGIVVEKIAYKPLRRATPLAVLITAIGVS
                     YFLQNAALLLFGADTKSFSSVVSLPSLKLADGALTISGTTIVTVLACVVIMIALMMFI
                     KKTKAGQAMLAVSEDKDAAQLMGVNVNATISLTFAIGSGLAAIAGVLFCSAYPTLTPY
                     TGSMPGIKAFTAAVFGGIGSIPGALIGGILLGIIEILGRAYISSQLSDAIVFAVLIVV
                     LLVKPTGILGKQIHEKV"
     gene            complement(4393..>5201)
                     /locus_tag="ACZO9R_02395"
     CDS             complement(4393..>5201)
                     /locus_tag="ACZO9R_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0911955.1"
                     /translation="VEKTAEDGMYELTPSASSTDVIDNDNVFQVCFTDPNQGTASAQY
                     IGENKLAAKVAVIYDSSDVYSSGIEANFVKEAQNQGLEVVAEEAFTADSKTDFSTQLQ
                     KAQSAGAELVFLPIYYTEASIILTQADSMGYAPTFFGCDGLDGLLGVENFNTELAEGV
                     MLLTPFAADADDEKTQSFVTKYKEAYGDTPNQFAADAYDAIYIIKAAAEQAGVTPDMS
                     VADIGSALEAAMAEISVDGLTGDSMTWQATGEVSKAPKAVVIKDGAYSAM"
BASE COUNT         1285 a         1459 c         1099 g         1358 t
ORIGIN      
        1 tgtttttgct tcccaggaca cctttcctgt gaagcgaaaa aagtgtccca ccgcttgcat
       61 gcggcagaac acttttatta tagcatggat tctatttctg gtaaatctga ataaaatttg
      121 tgaccacttc cacaagcttt tcatagctca tggacgtcgt gtccagacag aaattataat
      181 ttctggcgtc attccagtct cttccggtat aatagcggta atattccgcg cggtacttgt
      241 cgattttctc aatccgtctc tccgcttcct tctccgagag gccgttctgg ttcatcactc
      301 gtgcgacgca gtccttcttc ggcgcacaga aatacagttt gatgacatcc ggatcatccc
      361 ggagtaaata atccgcacac cggccgataa tcacgcagga ttcctgcgct gccagctctt
      421 tgatgacctt cgcctgataa ttgaacaggt tatcatcgga cacaaaatcc gaattctccg
      481 gcggaatcac accacccttg tacggatttt tcttcaaaat tccaaacaac ggagaccgct
      541 tcaaccgctc gtctgcctgt ccgaaaagtg cctcgttgat gccgctggca tccgatgcca
      601 ttcttaaaat ttccctgtca tagcagttga ttccaagctg ctcagccaaa agctttccca
      661 gtgttcttcc accacttcca tagcttctcg caatggtaat gactgttttt ttcattccat
      721 tctacctccc tgcattttcc gtctattctc ccagatatgc cttcttaata gaatcattgt
      781 tcatcatttc cttcgcgtca ccggagagaa caatatttcc ggtctcaagc acatacgcgc
      841 ggtttgcgat cgcaagcgct ttcttcgcat tctgctccac cagaagaacc gtcgtaccgc
      901 tcgctgagat ctcccggatg atatggaaaa tctcctccac aaaaatcggg gaaagtccca
      961 tggacggctc atccatcaga atgatcttcg gcttggacat cagggcgcgc cccattgcaa
     1021 gcatctgctg ctcgccgccg cttaacgttc ctgcaagctg gttttttcgt tcttccagac
     1081 gtgggaaact cttgtaaacc tttgccagcg attccgcaat ctcgtttttg tcttttctgg
     1141 tataagcgcc cagcttcaga ttctcgtaga cggaaagttc cgcgaacacg cgccgtccct
     1201 ccggcacatg cgccattccc atcgatacga tcttatgtcc cggcaccttc acgatatcct
     1261 gtccctcaaa aaggatctca ccctcggatg gctttaacat tcccgttatc gtctgaagtg
     1321 tcgtcgtctt ccccgcaccg ttcgctccga tcagcgcaat gacttcccct tcgtttactt
     1381 caaacgagat ccccttaatt gcctgaatga caccataatg tactttcaga ttttttactt
     1441 ccaacattgc catagtcgtt cctccatcta tctcatgtca ttctccaaga tatgccttga
     1501 ttacctgcgg gtcatgaagt acatcacccg tcgcgccctc cgcaagtacc tgaccgaagt
     1561 taagcaccgt cagcttctca cagataccgg acaccagttt catatcatgc tcgatcagaa
     1621 ggattgtcat atcgaactca tcccgcacaa aatggatcgt ttccatcagc tctttcgtct
     1681 cgttcgggtt catgcctgcc gccggctcgt cgagcagcag cagctttgga ttcgttgcaa
     1741 gtgcacgcgc aatctcaagc tttctctgct ttccgtatgg aagattggag gacaggaaac
     1801 ccgcctcgcg ctccagatca aacactttta ataattccat tgccttctca tccatctccg
     1861 cctcaacctt gcggtatttc ggcagacgta agattccggt cagcgtcgag taggacatct
     1921 ggttgtgcag tccgatcttt acattgtcaa gcacgctctg atccttgaac agacggatgt
     1981 tctgaaatgt acgcgcaata cctgccttgt tgatgtcgat cgttttcttt cctgtgatat
     2041 ccgcaccatc caggctgata gttccctcat ccggcgtata cacaccggta agcatattaa
     2101 ataccgtcgt cttgccggca ccgttcggtc cgatcagacc gtacaactgt cctttctcga
     2161 tggaaatatg aaaatcatct accgcacgaa gtcctccgaa ggagatgcca agattcttta
     2221 cctctaacat tgccataact atactgcctc cttctttgtg tttttgcgga acttctgtgc
     2281 cattttctgt ctccattcaa tgcacttcgg agcccagttg aaaagcatca tgacgatcag
     2341 cacgatcgag tagattaaca tacggtaatc ggacagtcct cttaacacct ccggaagaag
     2401 ggtcaaaaga acggctgcaa tgatggaacc acggaaatta cccataccac caagtacgac
     2461 gaataccaga atcatgatgg acatattgta gccgaagttc gccggcgtcg ccgtaaccgt
     2521 ggaaaggttg tgcgcataga gcacgccgcc cgctcccgca atcgccgcag agatcgtgaa
     2581 tgccatcagc ttgaacttgg tgatgttgat gccgacagac tctgccgcaa tacggttatc
     2641 acggattgcc atgatcgcac ggccgcttct ggaattgacc agattgtata caacaaataa
     2701 agaaatcaga attaagatca cgcccgctgt aaagctggag agatgcggaa tctttgcgat
     2761 tcccttcgca ccgttaatca gccagacacc gtcatctgcg agatcaatcg cgtttccgtt
     2821 cgagattgca aagtggaatc cgcttccgtc ttttgcgata taaagaacgt tgatgatatt
     2881 tttgatgatc tcaccaaacg ccagcgtcac aatcgcaaga taatcgccgc gcagacgtaa
     2941 gaccggaatg ccgatcagaa atccgaagaa tgccgccatc gcagtgccca cgagcagcgc
     3001 aatgataaag agcacaatct ggctgccgat cacaccttcc agcatcttgg tgcagaaggc
     3061 actggaaaaa gctcccacac acatgaagcc cgcatgaccg atactcagct ctccgaggta
     3121 tcccacgcac agattcagac cgatcgccac gatagagtag gtgcacatgg gaacgagcag
     3181 tccctgcatc agcgaagaaa ttgctccggc agctaccatg atctgaacca caatatatac
     3241 cgcaatcacc aacacaaagg tgatcagctg gtcttttgtt tttttattca ccgtttttaa
     3301 atttaacttt gccatagctg ttctcccttt tgcattatac tttctcatga atctgttttc
     3361 caagaattcc ggtcggtttt acaagaagca caacaatcaa aaccgcaaat acaatcgcat
     3421 ccgaaagctg ggaggatatg taggcacgtc ccagaatctc aataattcca aggagaattc
     3481 cgccgatcag tgctcccggg atcgatccga ttccgccgaa aacagcagcc gtaaatgctt
     3541 tgatacccgg catggaaccg gtgtacggtg tcagcgtcgg atacgcggaa cagaacagca
     3601 cacctgcgat cgccgcaagt ccggaaccga tggcaaaggt cagggaaatc gtcgcattga
     3661 cattgactcc catgagctgt gccgcatctt tatcctcgga taccgcaagc attgcctgtc
     3721 cggctttcgt cttcttaatg aacatcatca gtgcgatcat aatcaccacg catgccagca
     3781 cggtgacaat ggttgtgccg gaaatggtca acgcgccgtc tgccagcttt aaggaaggca
     3841 aggacacaac ggaactgaat gatttcgtat ccgctccaaa cagcagcaat gccgcattct
     3901 gcaggaaata gctgacaccg atcgctgtga tgagcacggc aagcggcgtt gctcttcgca
     3961 gcggcttgta ggcaattttt tcaacaacaa ttccgagaac ggtacatacg atgatggaga
     4021 gaaccaccgc cggaagcggg ctgatcccct ggccgctcat tgcaaggaac accacatagc
     4081 acccgatcat aatgacatcg ccatgtgcaa aattcagcat tttcgcaata ccatatacca
     4141 tggtatagcc gagtgcaatg atcgcatata cgcttcccag actgattcca ttgatcagat
     4201 aggataagaa attcataaca acacctctgt ctttccttgt ttaatacgtt aaaacgtcag
     4261 cagtttcact tattataaca tgttacatac ctgtatacaa cttaaaaccg aaaaaagaag
     4321 aggttccgcg ccggcgggta gtcgcccccg ccaacgcgcc aacctcttta ttggcctttc
     4381 ctgcctgctc tattacattg ctgaataagc accgtctttg attacgactg ccttcggcgc
     4441 cttgctgacc tcgccggttg cctgccatgt catggaatca ccagtcaaac cgtcaacgct
     4501 gatctctgcc atagccgcct caagtgcact gccaatatct gccacgctca tatccggtgt
     4561 cacgcctgcc tgctctgccg ctgctttgat aatgtagatt gcatcgtaag catctgctgc
     4621 gaactggttc ggtgtatccc catatgcttc tttgtacttt gtaacaaagg actgtgtctt
     4681 ctcatcatct gcatctgctg caaacggtgt caagagcatt acgccctctg ccagctctgt
     4741 gttaaaattc tcaacaccaa gcagtccgtc cagaccgtcg catccaaaga atgtcggagc
     4801 atatcccatg ctgtctgcct gtgtcaagat gatggatgcc tctgtgtagt agatcggaag
     4861 gaatacaagc tctgcacctg cgctctgtgc cttctgaagc tgtgtggaga agtctgtctt
     4921 ggaatctgcg gtaaacgctt cttctgcaac aacttcaagt ccctgattct gggcttcttt
     4981 tacgaagttc gcctcgatac cggaagaata tacatcggaa ctgtcgtaga tcactgcaac
     5041 cttggctgcc agcttgttct caccgatata ctgtgcggat gcagtaccct ggttcgggtc
     5101 ggtaaagcat acctgaaata cattatcatt atcgattaca tccgtggaag atgcggacgg
     5161 tgtcagttca tacatgccgt cctctgcggt cttctcaaca a
//
LOCUS       DBMDQJ010000074         5194 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6241_length_5194_cov_1.727862, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000074 DBMDQJ010000000
VERSION     DBMDQJ010000074.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5194)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5194)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5194
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6241_length_5194_cov_1.727862"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..983)
                     /locus_tag="ACZO9R_02400"
     CDS             complement(<1..983)
                     /locus_tag="ACZO9R_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016297710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR0911956.1"
                     /translation="MALLQHAVAVAREYLWGFPMLALLFGTHLYFTFHLGFIQKKIPL
                     GIRLSLTGTKNQEKDQVTPYSALATALAATIGTGNIVGISTAIAIGGPGAVFWCWLTG
                     VFGIATCYAECYLSIRYRVRRADNTFAGGPMYILDRRLKKRKLAAAFAVCTLLASFGI
                     GSSVQSYSIRTALQHQITVSPHLVGMAVGVLAGIIIIGGNSQIAKVCMWLVPLMSAFY
                     VGGCLYIIGRNRAVLPETLALILSSAVCPKAVAGGCTGGALLLGIQTGVSRGLFTNEA
                     GLGSIPMVAATAASSSDSPKEAAVRQALISMTGPFWDTVVLCAITGIAAVG"
     gene            complement(1063..1668)
                     /locus_tag="ACZO9R_02405"
     CDS             complement(1063..1668)
                     /locus_tag="ACZO9R_02405"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR0911957.1"
                     /translation="MSRIITIGREFGSGGREIGKRLSDELGIAYYDNEIISQIADRTK
                     LAEGYVQHVMENGPSTLYPITIGRSFYMGVDPIMEQNNAIYREQSRLIQEFAEKSDCV
                     IVGRSADYILREKHPFRLFVYAEMEHKMERCKKRAPESEHFTDKELKRHILDVDKRRA
                     RYYQFFTGQTWGDRLNYDLCINTSNYDIKELVHAIAHLVEA"
     gene            complement(1832..2281)
                     /locus_tag="ACZO9R_02410"
     CDS             complement(1832..2281)
                     /locus_tag="ACZO9R_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subtilin biosynthesis sensor protein SpaK"
                     /protein_id="HIR0911958.1"
                     /translation="MKYTTTDELEHFSFAEAYIADVQLADGFFHMTLSNVTILPENSC
                     NRDIREMRANNLVLKIEEPVIRSLVREGYKVYDANGALMKNCADEVIDQAAWNETIRS
                     FADGTLYALKQDGENYVFEIDAPDEEEYVLAMSGTHNTASWDRFLNK"
     gene            complement(2371..3594)
                     /gene="hydF"
                     /locus_tag="ACZO9R_02415"
     CDS             complement(2371..3594)
                     /gene="hydF"
                     /locus_tag="ACZO9R_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HIR0911959.1"
                     /translation="MAVTLNETPGSSRLHIGIFGKTNSGKSALINAFTGQEVSIVANV
                     AGTTTDPVYKAMEVHPLGPCIIIDTAGFGDESELGKSRMEKTRLAAEKTDIALLLVAG
                     NGDAALDEEEMHWYRFLKEHGTPVLFVVGKTDIRSDAQVDKLAAAIRGKTGEEAYPVS
                     AKTGEGMEALKEALIRKLPENYGSRLITGTLVGREDLVLLVMPQDIQAPKGRLILPQV
                     QTLRELLDKKCLVMSVTTDQLIPALAVLKKAPKLIITDSQVFGYVYENKPAESMLTSF
                     SVLFAAYKGDLPYYVEGAEHIDALNAHSRILIAECCTHAPLKEDIGREKIPRMLRKRF
                     GESLAVDIVSGTDFPKDLSGYDLIIQCGACMFNRKYVMSRIGHAREQGVPMTNYGVTI
                     AHLTGILDKISMPET"
     gene            complement(3694..5118)
                     /gene="hydG"
                     /locus_tag="ACZO9R_02420"
     CDS             complement(3694..5118)
                     /gene="hydG"
                     /locus_tag="ACZO9R_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HIR0911960.1"
                     /translation="MYKYDAKSLKAEEFINDEEIRETLAYADANKDNVELIDAIIAKA
                     GERKGLNHREASVLLACQIPEKIEEVYALAQQIKKDFYGNRIVLFAPLYLSNYCVNGC
                     VYCPYHLKNKHIARKKLTQEEIVKEVTALQDMGHKRLAIEAGEDPVNNPIEYILECIQ
                     TIYSIKHKNGAIRRVNVNIAATTVENYRKLKEAGIGTYILFQETYHKESYENLHPTGP
                     KHDYAYHTEAMDRAMEGGIDDVGLGVLFGLEMYRYEFAGLLMHAEHLEAVHGVGPHTI
                     SVPRVKHADDIDPTAFDNGISDDIFAKICALIRISVPYTGMIISTRESQAVREKVLPL
                     GVSQISGASRTSVGGYAEPEPEEENSEQFDVSDQRTLDEVVNWLMKMGYIPSFCTACY
                     REGRTGDRFMALCKNMQILNCCHPNALMTLKEYLEDYAGEETKKIGMELIDRELEKIP
                     NPKVKQTAYEHIHDIAEGKRDFRF"
BASE COUNT         1082 a         1573 c         1186 g         1353 t
ORIGIN      
        1 ctgccgaccg ccgcaattcc cgtgatcgca cataatacca ccgtgtccca gaacggtcct
       61 gtcatggaga ttaacgcctg ccgcacagcc gcctccttcg ggctgtcaga ggaggatgct
      121 gccgtcgccg cgaccatcgg aatcgagcca agccctgctt cattggtaaa caatccccgg
      181 gagactcctg tctggatgcc aagaagcagc gctccgccgg tgcagccgcc cgccacagcc
      241 ttcgggcaga cggcggatga gagaatcagc gctaacgtct ccggcagcac cgccctgttt
      301 cttcctataa tatagagaca gccgcccaca taaaaagcgc tcatcagcgg gacgagccac
      361 atacacacct ttgcaatctg gctgtttccc ccgatgataa taatccctgc aagcaccccg
      421 accgccattc cgaccagatg cggcgacacg gtaatctgat gctggagcgc cgtgcgtatg
      481 gagtatgact gtacgctgct tccgatacca aacgatgcca gcagcgtaca tacagcaaac
      541 gccgctgcca gttttctttt tttcagacgc cgatccaaaa tatacatcgg tccgccggca
      601 aacgtattgt ctgccctgcg cacccggtat cttatggaga gataacattc cgcatagcag
      661 gtcgctatcc cgaatacccc ggtcagccag caccagaata ccgctcccgg tccgccgatg
      721 gcaatggctg tggaaattcc cacaatgtta cccgtaccga tcgtcgctgc aagcgccgtc
      781 gccaacgccg aatacggtgt tacctgatcc ttttcctgat tcttcgttcc ggtcagtgac
      841 aaccggattc ccaacgggat ttttttctgg atgaagccca ggtgaaatgt gaaatataga
      901 tgtgttccga ataagagtgc aagcatggga aatccccaca gatattcccg cgccaccgcc
      961 accgcatgct gcaataatgc cataaaaatt ccccatatac cctgcatcac aaatatctgt
     1021 aaagtatatg gggaatctgt ttttcccatg cattcgattg ttctatgctt ctaccagatg
     1081 tgcgattgca tgtaccagct ccttgatgtc atagttggaa gtattgatac acagatcgta
     1141 attgagacgg tctccccagg tctgtccggt aaagaactga tagtagcgcg ccctgcgctt
     1201 atccacgtct aaaatatggc gctttaattc cttgtccgtg aagtgttcgc tctccggcgc
     1261 gcgcttcttg cagcgctcca tcttgtgctc catctccgca tagacaaaca gacggaacgg
     1321 atgcttctcg cgcaaaatat aatcggcgct tcttccgacg atgacacagt cggacttctc
     1381 cgcaaattcc tgaatcaggc ggctctgctc acggtagatc gcattattct gctccatgat
     1441 cggatcgact cccatgtaaa agcttctccc gatcgtaatc ggatacagcg tcgacggacc
     1501 gttctccatc acatgctgca catagccctc cgccagcttc gtacggtctg caatctgact
     1561 gatgatctca ttatcgtaat atgcaattcc cagctcatcg gacaatctct ttccgatctc
     1621 acgtccgccg ctgccaaatt ctcgtccaat cgttataatc ctagacattg gtatctcctt
     1681 tcccctgtgt ggggtttgtt tttctatcta aaactatagc atttcttcct gcggttcgca
     1741 actgcaaaag ctgccaccgc agataatagc cgatccctgc cgcatcatct gcgcctgttt
     1801 tcctccctga cgccttttct tcccggcacg gctacttgtt cagaaagcgg tcccaggacg
     1861 ccgtattgtg tgtgccagac atcgcaagca catactcttc ctcatcgggc gcgtcgatct
     1921 caaatacata gttctccccg tcctgcttta acgcatagag cgtgccgtcc gcaaaggagc
     1981 ggatcgtctc gttccacgcc gcctgatcga tcacctcatc cgcgcagttt ttcatcagtg
     2041 cgccgttggc gtcgtagacc ttgtatccct cccgcaccag tgaccggatg accggctcct
     2101 cgatctttaa caccagattg ttcgcgcgca tctcgcggat atcccggttg caggaattct
     2161 ccggcaatat cgtcacattg gagagcgtca tatggaaaaa tccgtctgcc agctgcacgt
     2221 cggcaatgta ggcttccgca aaagaaaaat gttccagttc atctgttgtc gtgtatttca
     2281 tagtgttcct ccgtatctct tccctgttat ttctcctccc ctattataca gagccgtccc
     2341 cgttttttcc accaaaaaga gtaacattgt ctatgtctcc ggcatggaaa tcttatcgag
     2401 aatccccgtc agatgcgcga tcgtcacgcc gtagttcgtc atcggaacgc cctgttctct
     2461 ggcgtgcccg atccgcgaca tcacatattt gcggttaaac atgcacgcgc cacactggat
     2521 gatgagatcg taaccggaaa ggtctttcgg aaagtcagtc ccgctcacaa tatccacagc
     2581 aagcgactcg ccgaagcgtt ttctcaacat ccgcggaatc ttttcccttc cgatgtcctc
     2641 cttcagcggt gcatgggtac agcactccgc gatcagtatc ctggaatggg cgttcagcgc
     2701 gtcaatatgc tccgccccct ccacatagta cggcagatcg cccttatatg ccgcaaacag
     2761 cacggaaaag gacgtgagca tgctctccgc cggtttgttt tcgtagacat agccaaatac
     2821 ctgggagtcc gtaatgataa gcttcggtgc ttttttcaat accgcaagcg ccgggatgag
     2881 ctgatcggtc gtcacgctca tgaccaggca tttcttatcg agaagttcgc gcagggtctg
     2941 cacctgcggc agaatcagcc ttcccttggg tgcctggata tcctgcggca tcacgagaag
     3001 caccagatcc tcccggccga ccagtgtccc ggtgatgagg cggctgccgt aattctccgg
     3061 cagtttgcgg atcaatgctt cttttaatgc ctccatgccc tctcccgtct tggcgctgac
     3121 cggatatgcc tcctcaccgg tcttcccccg gatggcggct gccagtttat ccacctgtgc
     3181 gtccgaccgg atgtccgttt tccccacgac gaaaagtacc ggcgtcccgt gctctttcaa
     3241 aaagcggtac cagtgcattt cttcctcatc aagcgccgcg tcaccattcc ccgccacaag
     3301 gagcagcgca atatccgtct tttccgctgc gagacgtgtc ttttccatgc ggcttttgcc
     3361 cagttcgctc tcgtcgccaa atcctgccgt atctatgata atgcacgggc cgagcggatg
     3421 tacctccatc gccttgtaga ccgggtcggt cgtcgttccc gccacattgg cgacaatgga
     3481 tacctcctgt ccggtaaaag cgttgatgag tgcggatttc ccgctgtttg tttttccaaa
     3541 tattcctatg tgcagacggc tgctgcccgg cgtctcattc aatgtcactg ccataacttt
     3601 cttttccctt cctgcatacg aatttcccct tgtccgtatt acatccatat ccggcttcca
     3661 gcggctgccc gatcccagca tcgcttccgg ctatcagaag cggaagtctc tctttccctc
     3721 tgcaatatca tgaatgtgct cgtaggcggt ctgctttacc ttcggattcg gaatcttctc
     3781 aagctcacgg tcaatcagtt ccataccgat ctttttcgtc tcctcgcccg cataatcctc
     3841 gaggtattct tttaaggtca tgagcgcgtt cggatgacag cagtttaaga tctgcatatt
     3901 tttgcagagc gccataaaac ggtcaccggt tctaccctcg cggtaacatg cggtgcagaa
     3961 gctcggaata tagcccattt tcatcagcca gttcacgacc tcatctaagg tacgctgatc
     4021 gctgacgtca aactgctcgg aattctcctc ctccggctcc ggctcggcat aaccgccgac
     4081 actcgttctc gatgcaccgc tgatctgcga tacgccgagc gggagcactt tctcacgcac
     4141 tgcctgactc tcgcgtgtgg agatgatcat tcccgtatac ggcaccgaga tgcggatgag
     4201 cgcacagatc tttgcaaaaa tatcatcgct gataccgttg tcaaaagcag tcggatcaat
     4261 gtcatcggca tgtttgacac gcggcacgct gatcgtgtgc ggtccgacgc cgtgcacggc
     4321 ttccagatgc tccgcatgca tcagaagtcc ggcaaattca tagcggtaca tctccagtcc
     4381 gaacaacacg cccagtccga catcatcgat gccgccctcc atcgcacggt ccattgcctc
     4441 cgtgtggtac gcgtaatcat gcttcggtcc ggtcggatgc aggttctcat aactctcctt
     4501 gtggtaggtc tcctgaaaca ggatgtaggt tccgattccg gcttctttta acttgcggta
     4561 gttctcgacc gtcgtcgccg caatattgac gttgacacgg cggatggctc cgtttttgtg
     4621 cttgatactg tagatcgtct gaatacactc cagaatatat tcgatcggat tgttcaccgg
     4681 atcttcgccg gcctcgattg ccagacgctt gtgccccatg tcctgaagtg ccgtgacttc
     4741 cttcacgatc tcctcctgtg tcagcttctt tctggctatg tgcttgtttt tcaggtgata
     4801 cgggcagtat acacaaccgt tcacacagta gttggaaaga tacagcggtg caaacagcac
     4861 gatacggttg ccgtaaaagt ctttcttgat ctgctgcgcc agcgcataga cttcctcgat
     4921 tttttccgga atctgacagg cgagcagcac cgatgcctct ctgtgattta agcccttgcg
     4981 ctctcccgct tttgcgatga tcgcgtcgat cagctccaca ttgtccttgt tcgcatccgc
     5041 gtaggcaagc gtctcccgga tctcctcatc gttgataaat tcctctgctt ttaatgattt
     5101 tgcgtcatac ttgtacataa tttttccttt ctttccggtc agttcatgct tcccggtcag
     5161 tgtttttatt gtaaaccgca ggacgatagt cccc
//
LOCUS       DBMDQJ010000075         5157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6293_length_5157_cov_1.533030, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000075 DBMDQJ010000000
VERSION     DBMDQJ010000075.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_5157_cov_1.533030"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="ACZO9R_02425"
     CDS             <1..442
                     /locus_tag="ACZO9R_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0911961.1"
                     /translation="YEKDGVRIGTHRLMGLTEILAQRGCYKEHYFCVGEISRESGYRL
                     TRELLETKDVPEVLVLGNDEVAEGALEAVKELKYRIPKDVAVVIYRDIETLQTKYPTY
                     TSLRMLPDIVWSTAIKLLLERIIDKRVDSMKVYLPTKLELGDSA"
     gene            480..1592
                     /locus_tag="ACZO9R_02430"
     CDS             480..1592
                     /locus_tag="ACZO9R_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR0911962.1"
                     /translation="MKRLTATLLAAAMCMGTFAGCGSNTEETTDNSNAGAAAGETAAA
                     TTESQTASAENVHLDALNVYFVPSRDPEEIVTATEPLSDLLKNELAGLGYDVDSVNIS
                     VGTTYEAVGEALSAGTADVGFIPGGTYVLYDDGCDVILTATRDGLNKDSDNPADWNDG
                     QPTEASDKQAVSYRALFIAGPSEKGQELADKVNSGEELTWDDLDSANWSVMSSSSPAG
                     YIYPALWMQDRYGKGITDLSNAVQSDSYASAFARLASGQVDVLVTYADARRDYEENWT
                     SEYGRSASIWEETNVIGVTDPIYNDTVCVSKTSPYMDENLKTAIQTALINIGNTEEGK
                     AVIAIYSHNGYEPAQSSDYDKEREAQKLIQELNAAN"
     gene            1699..2460
                     /gene="phnC"
                     /locus_tag="ACZO9R_02435"
     CDS             1699..2460
                     /gene="phnC"
                     /locus_tag="ACZO9R_02435"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR0911963.1"
                     /translation="MIEFSNVSKVYPNGVVGLDDVNLTIEQGEFVGIIGLSGAGKSTL
                     LRTINRMHDITSGTLTVDGQEVKNLKGKQLRKFRRNIGMIFQSFNLVTRTTVIRNVLM
                     AKVPEMPFWRVLLGIFKKEDKLNALEALDKVGILDKAYIRADQLSGGQQQRVALARTL
                     AQNPKIILADEPVAALDPVTAKQVMSDFKKINQEMNITILINIHHVELALAYADRIIG
                     IRAGKIVYDGPSADVTEDVLNMIYEGKIPDEVEEA"
     gene            2460..3281
                     /gene="phnE"
                     /locus_tag="ACZO9R_02440"
     CDS             2460..3281
                     /gene="phnE"
                     /locus_tag="ACZO9R_02440"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR0911964.1"
                     /translation="MGIYDKIFKPKKLVLSDGKVVEEKRTRTPLVLFLLLIATVISVR
                     ITGFSLNTILTRGNQFFVILGQMFPPKTGYLSSIWGPLLDTIKMSLLGSFAGGVLAIP
                     FAILASSNLVKNKVVLGVVRIFLSIVRTIPTLVAALIATYIWGLGTMAGTVAIAVFTF
                     AYVGKQLYELIETVDMGAYEAMEAMGAGRAQAFLAAIQPQVLPAYLSVCLFCFEGNVR
                     YAAILGYVGAGGLGLILNEKIGWREYDSVGMILVVLFGTVLVIEALSHYIRKKLT"
     gene            3298..4107
                     /gene="phnE"
                     /locus_tag="ACZO9R_02445"
     CDS             3298..4107
                     /gene="phnE"
                     /locus_tag="ACZO9R_02445"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HIR0911965.1"
                     /translation="MKKEIDKMYEQEPKTWYAKILVALIVAALLAWSMSTVQSGTTGG
                     DGLTVAGKIISGIFHPDTALLFNFTTSGVAYLLLETMCIAFLGTIVGAVLSIPLSFLA
                     AANLVPKPIALIFRVFIMAIRTIPAFVYGLMFIRVTGPGPFAGLLTMSLCSIGMISKM
                     FIESIEDLDTKILESLDAAGCTMFQKIRYGILSQLFPDFMSTLIYRFDMNLRDATVLG
                     LVGAGGIGAPLIFAMSAYKWNQVGAILAGLIVLILIVEVISTKIRVKLTRG"
     gene            4131..>5157
                     /locus_tag="ACZO9R_02450"
     CDS             4131..>5157
                     /locus_tag="ACZO9R_02450"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR0911966.1"
                     /translation="MEQTLKIYFTSDVHGYFYPTTYGDLKRKDLGLFSFARDFKKDEN
                     TLVIDGGDILQGSAFAYYCRQKSGSPQAIADIMNDCGYDYYTLGNHDFNYGMDYQNAY
                     IEAHHGACVCQNVVDEAGRACHPYVIHTLGNGLRVGIVGIVTDYVNVWERKENLAGIC
                     ITDPFEAAKEALLHLKKEVDITLCIYHGGFECDLKTGERLQKTTENVGYRICKELDFD
                     ILLTGHQHMSVDGQYVHGTYVVQPLENAKEYHYLEAERTKDGLVVRSEKRVADGANAV
                     GALCEKYAADEERVQSWLDQPIGHLSRALLPGEKAEMALHGSPIADFLNRIQLHFSGA
                     QLSAVGLA"
BASE COUNT         1355 a         1117 c         1461 g         1224 t
ORIGIN      
        1 ctatgagaag gatggtgtac ggatcggtac ccaccgctta atgggactga cggagatcct
       61 tgcgcagaga gggtgctata aggagcacta tttctgtgtg ggtgaaatca gcagagagag
      121 cggctaccgt ctgaccagag aacttctgga gacgaaggat gtaccggaag ttctggtgct
      181 tggaaatgat gaggtggcag aaggcgccct ggaagcggta aaagaattaa aataccggat
      241 tccgaaggat gttgcggttg tcatttaccg tgatattgag acgctgcaga cgaaatatcc
      301 gacatacacc agtcttcgga tgctgccgga tattgtatgg tcaacagcaa tcaagctttt
      361 gctggaacga atcatcgaca agcgcgtgga cagtatgaag gtttacctgc cgacaaaatt
      421 ggaactgggc gacagtgcat gaataattgt ttcactgcag agaggagaaa tttgcaatta
      481 tgaagagatt gacagctact ttactggcag cagcaatgtg catgggtaca tttgcaggct
      541 gtggaagtaa cacagaagag acaacagata acagcaatgc aggtgcagca gcaggcgaga
      601 cagcagctgc tactacggag agccagacag cttccgctga gaatgtgcat ctggatgcgt
      661 taaacgtata ttttgtaccg tccagagatc cggaagagat cgtaactgca acagagccgt
      721 tgagtgatct gttaaagaac gagcttgcgg gactgggcta tgatgtagac agtgtaaaca
      781 tttccgtagg tacgacatac gaggcagtag gcgaggcact ttccgccgga acagcggatg
      841 ttggatttat tccgggcggt acttacgtac tttatgatga cggatgcgat gtcattctga
      901 cagcaaccag agatggtctg aacaaggact ccgacaatcc ggcggactgg aatgacggac
      961 agccgacaga ggcatctgac aagcaggcag tatcttaccg tgctctgttc atcgcaggtc
     1021 cttctgagaa aggacaggag cttgcagaca aggtaaattc cggagaggag cttacctggg
     1081 atgatctgga tagcgcaaac tggagcgtga tgtcttcttc ttcaccggca ggctacattt
     1141 acccggcatt atggatgcag gatcgttacg gcaagggcat cacagatctt tccaatgcag
     1201 tacagtctga ttcctatgca agtgcattcg caagacttgc atccggacag gtagatgtat
     1261 tggtaaccta cgcagacgca agacgtgact acgaggaaaa ctggaccagc gagtatggac
     1321 gctctgcttc catctgggag gagacgaacg tcatcggcgt gacagatccg atctacaacg
     1381 atactgtctg tgtgagcaag acctctccgt acatggatga gaacttaaag acagccattc
     1441 agacagcact gatcaacatc ggcaataccg aagagggcaa ggcggttatc gcaatctaca
     1501 gccacaacgg atatgagccg gcacagtctt ctgactacga taaggaaaga gaagcacaga
     1561 agctgattca ggaactgaac gcagccaact aatcagaaac gggcagacaa agggtgcagt
     1621 gtcagcggag tgatcttgga aaagagacat cactccgctg tgctgcacat tttttaaggc
     1681 aatgtggaag gagcatttat gatagagttt tcaaacgtat ccaaggtata tcccaacggc
     1741 gtggttggac tcgacgatgt caatcttacg attgagcagg gcgagtttgt gggaatcatc
     1801 ggtctttcgg gagcaggaaa atcgacactg cttcgcacga tcaaccgtat gcatgacatt
     1861 acttccggta cgcttacagt ggacggacag gaggttaaga acctcaaagg aaaacaactg
     1921 cggaaattcc gccgcaatat cggaatgatt tttcagtcct ttaatcttgt gacacgcacc
     1981 acggttatcc gtaacgtatt gatggcaaaa gtgccggaga tgccgttctg gcgtgtatta
     2041 ctcggtatat ttaaaaaaga agacaagttg aacgctttgg aggcactcga caaagtgggc
     2101 attttagata aggcatatat ccgtgcggac cagctttcag gcggacagca gcagcgcgtg
     2161 gcgctcgccc gcacactcgc acagaatccg aaaatcatcc tcgcggatga gccggtagcg
     2221 gcgctcgatc cggtaacggc aaagcaggta atgagcgatt ttaagaagat caatcaggag
     2281 atgaacatta ccattttgat caatattcac catgtagaac tggcgcttgc ctatgcggac
     2341 cggatcatcg gtatccgcgc cggaaagatc gtctatgacg gaccttccgc ggatgtgaca
     2401 gaagacgtgt taaacatgat ctacgaggga aagataccgg atgaggtgga ggaagcctga
     2461 tgggaatata cgataaaatt ttcaagccga agaaactggt gttgtccgac ggaaaagtgg
     2521 tggaggaaaa acgcacacgc acgccgcttg tgctttttct gcttctgatc gcgaccgtca
     2581 tctccgtgcg gatcaccgga ttttcactca acacaatctt aacgcgcgga aatcagttct
     2641 ttgtcatatt agggcagatg ttcccgccga agacgggata tttaagcagc atctggggac
     2701 cgcttctgga cacgatcaag atgtcactgc tcggttcttt tgccggcggt gttctcgcaa
     2761 ttccgtttgc gattttagca tccagcaacc tggtcaaaaa taaagttgtt ctgggtgtgg
     2821 tgcgcatttt cttaagtatc gtgcgcacga ttccgacgct ggtggcggcg ctgattgcaa
     2881 cctatatctg gggactcggc acaatggcgg gaaccgtggc gattgcagtc tttacgtttg
     2941 cctatgtcgg caagcagctt tacgagctca ttgaaacggt tgacatggga gcttacgaag
     3001 caatggaagc gatgggagct ggcagggcac aggcatttct ggcagcgatc cagccgcagg
     3061 tgcttccggc atatctttcc gtctgcctgt tctgttttga gggaaatgtc cgctatgcgg
     3121 caatcctggg atatgtaggt gccggaggac tcggtctgat cttaaacgag aagatcggtt
     3181 ggagagagta cgacagcgtg ggcatgatcc tcgtggtatt attcggaacg gtgctcgtga
     3241 tcgaggcact cagccattat attcgtaaga agcttaccta ggaggggaca aggcagaatg
     3301 aaaaaagaaa tagataaaat gtatgagcag gagccgaaga cgtggtatgc gaagattctt
     3361 gtggcactca tcgttgcagc acttctggca tggtcgatgt ccacggtgca aagcggaaca
     3421 acaggcggcg atgggcttac ggtagcggga aagattatca gcggcatttt tcatccggac
     3481 acagcgctct tatttaattt tacaacctcc ggcgtggcgt atctgctgtt ggagacaatg
     3541 tgtatcgcat ttctgggtac gattgtggga gcagtgctct cgatcccgct gtcttttctg
     3601 gctgcagcca accttgttcc gaagccgatt gcactgattt tccgtgtatt tatcatggcg
     3661 atccgcacaa taccggcatt tgtatatgga ctgatgttca tccgtgtgac gggaccggga
     3721 ccatttgcgg gacttctgac gatgtccctg tgctcgatcg gcatgatcag caagatgttt
     3781 atcgagagta tcgaagacct tgacaccaag atactggaat cgcttgatgc agcgggatgt
     3841 acgatgttcc agaagatccg ttatggcatt ctgtcacagt tattcccgga ctttatgtca
     3901 acgttaatct atcgttttga catgaatctt cgtgatgcga ctgtgcttgg tctggtaggt
     3961 gccggcggta ttggtgcgcc gcttattttt gcaatgagcg cgtacaaatg gaatcaggtg
     4021 ggtgcaatcc ttgcaggact gatcgtgctg attctgattg tggaggtaat ctccacaaag
     4081 atccgtgtga agctgacgcg gggataacta taatacgact gagggataag atggaacaaa
     4141 cgttaaagat atattttacc tcggatgttc acgggtattt ttacccaacg acttacgggg
     4201 atttgaaacg aaaagatctg gggctgttct cgtttgcccg ggatttcaaa aaagatgaga
     4261 atacactggt aattgatggc ggagatatat tgcagggctc cgcctttgcc tactattgca
     4321 ggcagaagtc aggatcgccg caggccattg cggatatcat gaacgactgc ggctacgatt
     4381 attatacgct cggcaatcat gattttaatt atgggatgga ctatcagaac gcttacatag
     4441 aggctcatca cggcgcgtgt gtctgccaga atgttgtaga tgaggcggga agagcatgcc
     4501 atccgtatgt gatccacacg cttggaaacg gactccgggt cgggattgtc ggtattgtga
     4561 cagactatgt gaatgtgtgg gagcggaagg aaaatctcgc gggaatttgc attacagacc
     4621 catttgaggc ggcaaaagag gcgcttttgc atctgaaaaa agaggtggac attacgcttt
     4681 gcatctacca cggcggattt gagtgtgatt taaagaccgg agagcgcctg cagaagacga
     4741 cggagaatgt cggataccgc atctgtaagg aactggattt tgacattctg ctgaccgggc
     4801 accagcatat gtccgtcgac gggcagtatg tgcacggaac ttacgtggta cagccgcttg
     4861 agaatgcaaa agagtatcac tatctggaag cggagcggac aaaagacgga cttgttgtcc
     4921 gctccgagaa gcgtgtcgcc gacggagcaa atgctgtcgg tgcattatgc gaaaagtatg
     4981 ccgccgacga agagcgggta cagagctggc tggatcagcc gatcgggcat ttaagccggg
     5041 cgcttcttcc cggagaaaaa gcggaaatgg cgctgcacgg ttcgccgatt gccgattttt
     5101 taaaccggat acagttacat ttttccggcg cacagctgtc agcggtcggt cttgcca
//
LOCUS       DBMDQJ010000076         5143 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6317_length_5143_cov_1.794129, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000076 DBMDQJ010000000
VERSION     DBMDQJ010000076.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6317_length_5143_cov_1.794129"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(319..1335)
                     /locus_tag="ACZO9R_02455"
     CDS             complement(319..1335)
                     /locus_tag="ACZO9R_02455"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HIR0911967.1"
                     /translation="MKNNKHEQNELQRMMEGRAARMIAAAVTVFLCVLTLFEFVALKM
                     EWNVPALAVLSGDNGLWTREYQREELVMDGSTENILLADDGETPLTGSGENAKTNVGE
                     NGSGGTAQTGANTENGEDLLVEGTEAVRQEETETPDVSGTGKAEADVKETQTPEFSYY
                     IRINRKQNCVTIYTQDEEGEYTVPYKAMVCSTGLYNATPRGTYQISTKYLWRELYGGV
                     YGQYATRIHGGILFHSVPYYKRAKDTLCTDKYNKLGQQASMGCVRLTVEDAKWIAENC
                     PEGTTVEIYDGEDPGPLGKPEAQKLDKSDPDCHWDPTDPDEENPWQQTEMSVTESEQE
                     KQIK"
     gene            complement(1345..2664)
                     /locus_tag="ACZO9R_02460"
     CDS             complement(1345..2664)
                     /locus_tag="ACZO9R_02460"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR0911968.1"
                     /translation="MIKVVKFGGSSLASAEQFAKVGKIIQADKERRYVVPSAPGKRNA
                     KDTKVTDMLYACYALVEAEEDFRVPLMKIKDRYDTIINGLNLKLSLEEEFKKISENFK
                     NKAGVDYAASRGEYLNGIIMANYLGYEFVDAAEVISFEEDGTFDAEKTNKVLAERLEK
                     CERAVIPGFYGALPDGTIKTFSRGGSDITGSIVARAVHATCYENWTDVSGFLIADPRI
                     IKDPQPIKTITYRELRELSYMGASVLHEDAVFPVRKAGIPINIRNTNAPDDEGTWIVE
                     STCHQSKYTITGIAGKKGFVSVNIDKDMMNSEVGFGRKVLSAFEENNISFEHMPSGID
                     TMTIFVHQPEFEGKEQAVISAIHRLAKPDSIELEGDLALIAVVGRGMKSTRGTAGRIF
                     SALAHANINVKMIDQGSSELNIIIGVSNADFEPAIRAIYDIFVETQL"
     gene            complement(2798..3595)
                     /locus_tag="ACZO9R_02465"
     CDS             complement(2798..3595)
                     /locus_tag="ACZO9R_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0911969.1"
                     /translation="MGCYRRIDRALEHALHLPLGTGSRYVVISDCHRGEGTTNDNFLK
                     NAYLYEAAMEHYIKRGFFYLELGDGEELWENRCMDRIVHYHETVYEMFACLQSRNALC
                     RIYGNHNMELRKILPEAIILDNCEGGRDVCMIHGHQADFFNSVCWRLSRALVRYVWKP
                     LERSGVNDPTSAARNYKKAVRYEQCLAGWTRLHDRYLIAGHSHRPRLPENGELYLNAG
                     SCVHPGCITAIELEQMQMTLVKWKVATRPDMTMYVVREKLAGPVAIL"
     gene            complement(3600..4511)
                     /locus_tag="ACZO9R_02470"
     CDS             complement(3600..4511)
                     /locus_tag="ACZO9R_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4474 domain-containing protein"
                     /protein_id="HIR0911970.1"
                     /translation="MCIAFLVLFAALLILAVVHRVRAKKKVLALTPGERVRLLNELAE
                     PFGFAYLPAEDIFTSRLDAWQRDKGYEAKYDTMAVGAGMVIDAFPVYFNYEGRTWLVE
                     FWKGQYGINTGGEVGIYHAKGVIPPHAYHTAHFEAVSDAELPRICSCLDTAEETFYEI
                     CARHWWLTGFRMGVYTKPSRLRLATTLIFPEQEMALAFWEGLHKTGKPRSLFRICGRE
                     VSVRMNFSEEHGLIPGICRSVVLLWNRLLCRGFRIVTYPWKRTADRMVFLYYLLPGWF
                     RKILVLSGGRCKPRCRTRKPHRSCRGR"
     gene            complement(4538..>5143)
                     /locus_tag="ACZO9R_02475"
     CDS             complement(4538..>5143)
                     /locus_tag="ACZO9R_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR0911971.1"
                     /translation="VGGNIGIGVVIPGGQRPGGNQTAGEMQNSVNQLFETMRLAWLEL
                     IYWTRMYMMSVDSGADAKEQYAVEGRLLETADAITDVFAERLPVAVTRQLRNLLVEHV
                     EMTGQIIRTLKSGDKENYEQQIREWYANANQIATLLADQNPYFAGKETRNLLLNYLDM
                     TREIIEHQMNGEYDQSIDTFRDLSDLVLELADYLARGLLAR"
BASE COUNT         1149 a         1547 c         1108 g         1339 t
ORIGIN      
        1 acgatttacc caaaacgata ttaaggaaag agaggtacag accaccgaaa acgtaattgc
       61 attcgttttt ttctaactcc cgaagaacac acgtgacaat ccaaatcatg gaaagaggaa
      121 tcagtatcag atgagttaat cagatatgat gcgatatcca ccgtcataag aagagtgata
      181 caaaggtacg aaggaaacga agaagtccga aagagagagc aaaaaaagaa aagagaggta
      241 cagtcccatg gacgagatag tttagaaggc gacatcgatt caaatgaata ggatgtcgcc
      301 ttcgtcgttc tattttttct attttatctg tttttcctgc tcgctctccg tcacggacat
      361 ctccgtctgc tgccagggat tttcctcatc cggatcagtc ggatcccagt ggcagtccgg
      421 atctgattta tccagttttt gtgcctccgg tttcccgagc ggccccgggt cttccccgtc
      481 gtatatttca accgtagtgc cttccgggca gttctccgca atccattttg catcctccac
      541 cgtcagtctt acgcagccca tcgacgcctg ctgtcccagc ttgttatact tgtccgtaca
      601 gagtgtatcc tttgcgcgct tgtaataggg cacagaatgg aacaggattc ccccgtgaat
      661 ccgcgtcgcg tactgcccat acacaccgcc atagagttcc cgccacagat acttggtgga
      721 aatctggtaa gttccgcgcg gcgttgcgtt gtaaagtccg gtcgagcata ccattgcctt
      781 atagggtacc gtatactccc cctcctcatc ctgggtgtag atcgtgacgc agttctgctt
      841 gcgattgatg cggatataat acgaaaattc cggtgtctgc gtctccttaa catccgcttc
      901 cgcttttccg gttccgctta catccggcgt ctcagtctct tcctgccgca ccgcttctgt
      961 cccttccacc agcaaatctt ccccgttctc tgtgttcgca ccggtctgcg ccgtcccgcc
     1021 gcttccgttt tcccccacat tcgttttggc attctcacca cttcctgtga gcggtgtctc
     1081 tccatcgtct gcgagcagaa tgttttccgt gctgccgtcc atcaccagtt cttcccgctg
     1141 gtactccctg gtccacagtc cgttgtcgcc gcttagcacc gccagtgcag gcacattcca
     1201 ctccatcttg agcgcaacaa attcaaacaa tgtcagtacg cacaaaaaga cagtgactgc
     1261 agccgcaatc atgcgggcag ccctgccttc catcatacgc tgtaattcat tttgctcgtg
     1321 cttgttgttc ttcatatctt aatcttaaag ctgggtctcc acgaaaatgt catagatcgc
     1381 acggattgcc ggttcaaaat ccgcattgct cacaccgata ataatgttaa gctcagaaga
     1441 tccctgatcg atcatcttga cgttgatgtt tgcatgtgcc agtgccgaga agatacggcc
     1501 tgccgttcct ctggtcgact tcatgccgcg tcccacaacc gcgatcagcg ccagatcacc
     1561 ctccagctcg atactgtccg gctttgccag acggtgaatt gctgagatca ctgcctgctc
     1621 cttgccctcg aattccggct ggtgaacgaa gattgtcatc gtgtcgatgc cagacggcat
     1681 atgctcgaat gagatattat tctcctcaaa cgcggacaat acttttctcc cgaatccaac
     1741 ctcggaattc atcatatcct tatcaatatt gacggaaaca aaaccttttt tgccggcaat
     1801 accggtaatg gtgtattttg actgatggca ggtgctctcc acgatccagg ttccctcgtc
     1861 atccggcgcg ttggtattgc ggatgttgat cggaattcct gcttttctca ccgggaacac
     1921 tgcgtcctca tgaagcacgc tggcgcccat ataggaaagc tcgcgcagct cacggtatgt
     1981 gatcgtcttg atcggctgcg gatccttaat gatgcgcgga tctgcaatga gaaagcccga
     2041 cacatccgtc cagttctcat agcaggtggc atgtaccgca cgcgccacga tggatcccgt
     2101 gatgtcggaa ccgcctctgg aaaaggtctt gatcgttccg tccggcagtg ccccgtagaa
     2161 tcccggaatt accgcacgct cgcatttctc cagacgctct gccagcacct tgttggtctt
     2221 ctccgcgtca aaagttccgt cctcctcgaa ggaaatcacc tctgccgcat ccacaaactc
     2281 gtagccaagg taattcgcca taataatacc gttcagatat tcgccgcggg acgccgcata
     2341 gtcaaccccc gctttatttt taaagttctc actgatcttt ttgaattctt cctccagtga
     2401 cagcttcaga ttcagtccat tgataattgt gtcatagcgg tccttgatct tcatcagcgg
     2461 cacgcggaaa tcctcctccg cctccaccag cgcataacag gcatataaca tatctgtgac
     2521 cttggtgtcc ttggcattgc gttttcccgg ggcactcggc actacataac gtctttcctt
     2581 atccgcctga atgatcttcc ccacttttgc aaactgttcc gcacttgcca gagagctacc
     2641 gccaaacttc acaactttaa tcatttttct gctcctccaa ttaccagcta tcgtcaggga
     2701 cgcttccgtc ctcgctttaa cgatttaata taacgatata tatattatca taaatggcgg
     2761 ttccgtcaaa tgattttttt gaaaaatcgc gtttttccta caaaatggca accggtccgg
     2821 caagcttctc acgtaccaca tacatggtca tgtccgggcg cgtcgccacc ttccatttca
     2881 caagcgtcat ctgcatctgt tccagttcga tggcagtgat gcacccggga tgcacacagc
     2941 ttcccgcatt cagatacagc tccccgtttt ccggcagtct cggtctgtgt gaatggccgg
     3001 caatcagata acggtcgtga agtctggtcc agcccgccag gcactgttca taccggaccg
     3061 cctttttata attgcgcgcc gcactcgtgg gatcattcac gccggagcgc tccaacggtt
     3121 tccacacgta gcgcaccaga gctctggaga gcctccagca cacagaatta aaaaaatccg
     3181 cctgatgccc gtgaatcatg cagacatccc ttccgccctc gcaattatct aatataatcg
     3241 cctccggcag gatttttctc agctccatat tgtggtttcc atagatgcgg cacagcgcat
     3301 tccggctttg cagacaagca aacatctcat acacggtctc gtgatagtgc acaatccggt
     3361 ccatgcagcg gttttcccag agttcctccc cgtcccccag ttccagatag aagaatcctc
     3421 tctttatata atgttccatc gccgcctcgt acaggtacgc gtttttcagg aaattgtcat
     3481 ttgttgtccc ttcccctctg tggcagtcac tgatcaccac atagcggctg cccgtgccaa
     3541 gcggaaggtg cagggcatgt tccaatgccc tgtcaatcct tcgataacat cccatgtttt
     3601 cacctgcccc gacatgatct gtgcggtttc ctggttctgc agcgcggttt gcatcgcccg
     3661 cccgaaagca ccagaatctt ccgaaaccat cccggaagca gataatatag aaataccatt
     3721 ctgtccgccg tccgtttcca cgggtatgtc acgatcctaa atcctctgca cagcagcctg
     3781 ttccagagaa gcaccacgct tctgcagatt cccggaatca atccgtgttc ctccgaaaaa
     3841 ttcatccgca cactgacttc tcttccgcag atgcgaaaaa ggctgcgcgg ttttccggtt
     3901 ttgtggagac cttcccagaa cgccagcgcc atctcctgct ctgggaaaat cagcgtcgtg
     3961 gcaagtctga ggcgcgacgg ttttgtatac acgcccatcc gaaagccggt gagccaccag
     4021 tgtctggcac agatttcata aaacgtttcc tctgccgtgt ccagacagct gcagattctc
     4081 gggagttctg catccgatac cgcttcaaaa tgcgccgtgt gataggcgtg cggcggaatc
     4141 acgcccttcg catggtaaat tccgacctct ccgccggtgt tgatcccgta ctgtcccttc
     4201 cagaattcca ccagccaggt tcttccctca taattaaagt acacgggaaa tgcgtcaatc
     4261 accatcccgg cacccaccgc catggtatca tatttcgcct catatccctt gtcacgttgc
     4321 caggcatcca gccttgaggt aaaaatgtct tccgccggca gataagcaaa tccaaagggt
     4381 tccgccagtt cattgagcag ccgcacccgt tccccgggag tcaatgcaag aacttttttc
     4441 ttcgcccgca ctctgtgcac tactgccagg atcagcagcg ccgcaaatag taccagaaag
     4501 gcgatgcaca agaggcaccg cccgtttgca aagcacatca gcgcgccaga agtcctcttg
     4561 cgagataatc agcgagttcc agcaccagat cacttaaatc ccggaaggta tcaatgctct
     4621 ggtcatactc accgttcatc tgatgttcta taatctcgcg tgtcatatcc agataattca
     4681 acagaagatt cctcgtctct tttcctgcaa agtagggatt ctggtcagcg agaagtgttg
     4741 cgatctgatt cgcattagca taccactccc ggatctgctg ttcataattc tccttatccc
     4801 cggattttaa cgtgcggatg atctgccccg tcatctccac atgttccacg agcagattgc
     4861 gcagctgtct tgtgacagct accggcagcc gctctgcaaa aacatccgtg attgcatctg
     4921 cagtttccaa caggcgtccc tccactgcat actgctcttt tgcatctgcc ccggaatcca
     4981 cactcatcat atacatccgt gtccagtaga tcagttccag ccacgccaga cgcattgtct
     5041 caaacagctg attcactgaa ttctgcatct ctcccgctgt ctgatttccg cccgggcgct
     5101 gtccacccgg aatcaccact ccgataccga tgtttccgcc cac
//
LOCUS       DBMDQJ010000077         5131 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6332_length_5131_cov_1.519682, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000077 DBMDQJ010000000
VERSION     DBMDQJ010000077.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5131)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5131)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5131
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6332_length_5131_cov_1.519682"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1623
                     /locus_tag="ACZO9R_02480"
     CDS             <1..1623
                     /locus_tag="ACZO9R_02480"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911972.1"
                     /translation="QGVEAGNIHHIYVMGAWMVVAACFSLFCGVMGGKYGARASAGFA
                     RNLRKAMYENIQSFSFSNIDKFSTAGLVTRLTTDVTNLQNAYQMLLRMCVRAPISLIC
                     AMCMAFFIRPRLASIYLVAVIILGIILAIITVRAHHYFMEVFPKYDDLNASVQENVSA
                     IRVVKAYVREDYEKNKFRKASQNIYNMFVKAESILAFNNPVMQITVYSCILSISWVGA
                     KMIVGGTLTTGELMSLLTYCMNIMMSLMMLSMVFVMLAMSVASAERICEVLNEKSDIT
                     NPENADKTVPDGSITFDHVTFRYSRTSAKPVLSDIDLSIKSGETIGIIGGTGSSKSSL
                     VNLISRLYDVSEGRVLVGGKDVRSYDLDTLRNEVSVVLQNNVLFSGTIYENLRWGDEH
                     ATDEECRHACELACADEFIERFPDGYNTYIEQGGTNVSGGQKQRLCIARALLKKPKIL
                     ILDDSTSAVDTATDAKIRRAFLTEIPNTTKLIIAQRISSVQDADRIIVLDNGELNGFG
                     THEELLQTNAIYRDVYESQTGGGGDFDEGGAA"
     gene            1623..3557
                     /locus_tag="ACZO9R_02485"
     CDS             1623..3557
                     /locus_tag="ACZO9R_02485"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0911973.1"
                     /translation="MPQNEKNAKPIGGPGGRGGHGPRPNVKNPGKLFMRLLSYIMKNY
                     AVHCVIVVICIFITVLASVQGTWFMQTLIDGYILPLMKQSDPDFSGLAHAILRVAVFY
                     GIGAIAAYIYTRIMVNVSQGTLKHLRDDMFTHMEELPIRYFDTHSHGDIMSTYTNDID
                     TLRQMISQSMPQFLNSIISIVSVFISMLLLNIPLTLVTLAMVGVTLFATKKIGALSAR
                     YFIAQQKDIATVNGYIEEMMNGQKVVKVFTHEEESIADFNRLNDQLFHSADNANKFGN
                     ILMPVNAQIGNISYVLCAIVGGILALGGYGGFTLGKLASFLTYNKSFGQPINQLSMQL
                     NNIVMALAGSERIFALLDEQPEVDDGYVTLVRARQENGQIVESKERTGMWAWKHTHQA
                     DGSIDYIELKGDVVFDDVDFGYVPEKTVLHNVDLYATPGQKIAFVGSTGAGKTTITNL
                     INRFYDIQDGKIRYDGININKIKKDDLRHSLGIVLQDTHLFTATVMENIRYGKLDATD
                     EEVMAAARLANADTFIQQLPNGYDTLLTGDGANLSQGQRQLLAIARAAIADPPVLILD
                     EATSSIDTRTEKIVQDGMDKLMAGRTTFVIAHRLSTVRNSDCIIVLEQGRVIERGSHD
                     QLIEKHGKYYQLYTGNLAEG"
     gene            complement(4199..4708)
                     /locus_tag="ACZO9R_02490"
     CDS             complement(4199..4708)
                     /locus_tag="ACZO9R_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0911974.1"
                     /translation="MDRKKKRIHLSKENEKVFREILTEVCKNSRFLSSSQFMQHGSTS
                     VFRHSVAVAYVSFYIAEKYRIRVNCHALIRGALLHDYFLYDWHEKDDSHKWHGFYHAG
                     KALQNAMEDFALSEIEKETIRRHMFPLNPIPPRCREAWIVTLADKICSGGETVEGFWE
                     KFEEIRNPS"
     gene            complement(4798..>5131)
                     /locus_tag="ACZO9R_02495"
     CDS             complement(4798..>5131)
                     /locus_tag="ACZO9R_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911975.1"
                     /translation="IERLMRRTTTQYKYVDENGDTQNFGAVTTLSGTEIYCDMDDLEI
                     LNNPTGINLAEFTWDDVRSLGNPEIKIYAKSSTNKDALQEDVIQGVQEAIQEDAGDGI
                     VITIEVDR"
BASE COUNT         1302 a         1363 c         1214 g         1252 t
ORIGIN      
        1 cagggtgtgg aagcgggcaa catccaccat atctacgtca tgggtgcctg gatggttgtc
       61 gccgcctgtt tctccctctt ctgcggtgtc atgggcggaa aatacggtgc aagagcctcc
      121 gcaggttttg cacgtaactt aagaaaagcg atgtacgaga acatccagag tttttcgttt
      181 tccaacatcg acaaattcag taccgcaggt cttgtcaccc gtctgaccac agatgtgacc
      241 aacctgcaga atgcctacca gatgctgctg cgcatgtgtg tgcgtgcgcc gatcagtctg
      301 atctgcgcga tgtgcatggc gtttttcatc agaccgagac tggcgagcat ctatcttgtt
      361 gccgtcatca ttctgggcat cattctcgcc atcatcaccg tgcgcgcgca tcactatttt
      421 atggaagttt ttccaaagta cgatgaccta aatgcctccg tgcaggaaaa tgtatccgcc
      481 atccgcgtgg tcaaggctta tgtgcgtgaa gattacgaga aaaacaagtt ccgcaaagcc
      541 agccagaata tttacaacat gttcgtcaag gcggaaagca ttctggcttt caacaacccg
      601 gtcatgcaga tcaccgttta cagctgtatt ctttcgatca gctgggtagg tgcaaaaatg
      661 attgtcggcg gaacattgac aaccggagaa ctaatgagtc ttcttaccta ttgtatgaat
      721 atcatgatga gcctgatgat gctgtccatg gtgttcgtca tgctggccat gagtgtggca
      781 agcgccgagc gtatctgtga agtattaaat gagaagagtg acatcaccaa tccggaaaat
      841 gcggataaga ccgtgccgga cggaagcatc acctttgacc atgtgacctt ccgttacagc
      901 aggacatccg caaagccagt gctctccgat attgacctct ccataaaatc cggcgagacg
      961 atcggtatca tcggcggaac cggaagttcc aagtcctcgc tcgtcaacct catcagccgt
     1021 ctctacgatg tatccgaagg ccgtgttctc gtcggcggaa aagacgtccg ttcctacgat
     1081 cttgacaccc tccgcaacga ggtctctgtc gtattgcaga acaatgtgct tttctccgga
     1141 accatctatg agaatctccg ctggggagat gaacatgcga ccgacgaaga atgccgtcac
     1201 gcgtgcgaac tggcatgtgc cgacgaattc atcgagcgtt ttccggatgg ttacaacacc
     1261 tacatcgagc agggcggaac caacgtctcc ggcggtcaga aacagcgtct gtgtattgcc
     1321 cgcgcgctct taaagaagcc gaagattctg attttagacg acagcaccag cgcggtggac
     1381 accgctaccg atgccaaaat ccgtcgcgct tttctcacgg agattccaaa cacgaccaag
     1441 ctgattatcg cacagcggat ctcctccgtt caggatgcag accgcatcat tgtgctggat
     1501 aacggagagt tgaacggttt cggcacacac gaagaattat tgcagaccaa tgccatttac
     1561 cgcgatgtct acgagtcaca gaccggcggc ggcggtgatt ttgatgaagg aggtgctgcc
     1621 tgatgccaca gaatgaaaaa aatgcaaagc cgatcggcgg accgggcggc cgcggcggtc
     1681 acggtcccag accgaatgtc aagaatccgg gaaagctttt tatgcgcctg ctttcctata
     1741 ttatgaaaaa ttatgccgta cactgcgtca ttgtcgtgat ctgtattttt atcacggttc
     1801 tcgcaagtgt gcagggaacc tggttcatgc agaccttaat cgacggctat atcctgccgc
     1861 tgatgaagca gagcgatccg gacttttctg gtctcgcaca cgccatttta cgcgttgccg
     1921 tcttctacgg catcggcgct atcgcagcct acatatacac aagaattatg gtcaatgtct
     1981 cacagggtac tttaaagcat ctgcgcgacg acatgttcac ccacatggaa gagcttccca
     2041 tccggtattt tgacacacac tcccatggag acattatgtc aacttatacc aacgatattg
     2101 ataccctgcg ccagatgatc agccagagta tgccgcagtt tttgaacagc atcatctcca
     2161 tcgtgagcgt ctttatcagt atgcttcttc tcaacattcc gctgacgctc gtcacacttg
     2221 cgatggtcgg tgtcacactg tttgccacaa agaagatcgg tgccttaagt gcccgctact
     2281 ttatcgcaca gcagaaagat attgcgacgg tcaacggcta cattgaagag atgatgaacg
     2341 gtcagaaggt ggtcaaggtc ttcacccatg aagaggaaag tatcgccgat ttcaaccggc
     2401 tcaatgacca gctgttccac agtgcagaca atgccaataa gttcggtaac attttaatgc
     2461 cggtcaatgc acagatcggt aatatcagct acgtgctctg cgcgatcgtc ggcggtattc
     2521 ttgctctggg cggatatggc ggatttacgc tcggaaagct cgcaagtttt cttacctata
     2581 ataagagctt cgggcagccg atcaaccagc tctccatgca gcttaacaat atcgtgatgg
     2641 cactcgccgg atcagagcgt atcttcgctc tgctcgatga gcagccggaa gtggacgacg
     2701 gctacgtcac acttgtccgc gccagacagg agaacggaca gattgtggaa tccaaagagc
     2761 gtacgggtat gtgggcatgg aagcacaccc atcaggcaga cggcagtatc gattatattg
     2821 aattaaaggg cgacgtggta tttgatgatg tcgatttcgg ctatgttcca gagaagaccg
     2881 ttcttcacaa cgtagatctc tacgctacgc cgggtcagaa aattgccttt gtcggcagca
     2941 ccggcgccgg aaagacgacc atcaccaacc tgatcaaccg tttctacgat atccaggacg
     3001 gcaagatccg ctatgacggc atcaatatca acaagatcaa aaaagatgac ctgcgccact
     3061 ctctcggaat cgtgctgcag gacacccatc ttttcacggc tacggttatg gaaaatatcc
     3121 gttacggcaa gctggatgcc acagatgagg aagtcatggc agctgccaga ctcgccaacg
     3181 cagatacatt tatccagcag cttccgaacg gctatgacac cctgctgacc ggcgatggtg
     3241 ccaacctaag ccagggacag cgtcagctgc ttgccatcgc aagagctgcc atcgccgatc
     3301 cgccggttct gattctggac gaagcgacaa gttccatcga tacccgcacc gaaaagattg
     3361 tgcaggacgg tatggataag ctgatggcgg gacgcaccac gttcgtgatc gcgcaccgtc
     3421 tctcgaccgt gcgcaacagt gactgcatca ttgtcctgga acagggacgc gtcatcgagc
     3481 gcggcagcca cgatcagctg attgaaaaac acggcaaata ctatcagctg tataccggga
     3541 atttagccga aggctaaatt tccgtgtaat tccagcggaa gattaagttc ctgaaaaaat
     3601 agaactgtcg cactaagcac agcgtgcttt gctctgataa gtaatattac agagatatca
     3661 gagaacaaaa aagttccaga ataaacagac ggcggcagat tctttgaagc aaaattcatg
     3721 tatttcttcc atgccgtggc tggcatcccg ttctgtgtct cttccacgtt tgaacactca
     3781 cggagtgtgt ccgacaagcc gcagacaatg ggcggacttt ctggtgtccc taatggcacc
     3841 tcttcacaga caatataaaa ccgcgcgaac cgaactcacc ggcatctgct attgaaaata
     3901 gggcagatgc cgactcagac agcggcttcg cgcggttatg ttttgttcag aggtgccaac
     3961 aagggacaca cacgaaagtc ctacaaattg tctgccggct tgcggacaca gtccgtagag
     4021 tgctccaaac ttggaaagac acggatcatc acagatgcca gctgcggcat ggttagaaat
     4081 accggatttt gcggtcagaa tttgccgccg catgtttatc tggaactttt aagtatctat
     4141 ttctaataat attacttatc aaagcaaaga gcgatatgct tattgcggca attctatttt
     4201 atgaaggatt cctaatctcc tcaaacttct cccaaaatcc ttcgacagtc tccccgccgg
     4261 agcagatctt atccgccagt gtcacaatcc acgcctccct gcagcgcggc ggaatcggat
     4321 tgagcggaaa catatgccgg cggatcgtct ccttctcaat ctcactcaac gcaaaatctt
     4381 ccatcgcatt ctgcagcgct tttcctgcat gataaaatcc atgccactta tgggaatcgt
     4441 ccttctcatg ccagtcgtat agaaaatagt catgcagtaa cgccccgcgg atcagcgcat
     4501 gacagttcac gcggattcta tacttttccg caatgtaaaa actgacgtac gccaccgcca
     4561 cgctgtggcg gaatacgctc gtggaaccat gctgcataaa ctggctggag gagagaaacc
     4621 tggaattttt gcatacctcc gtaagaatct cccgaaaaac tttctcattc tcttttgaca
     4681 aatgaatccg cttctttttt ctgtccattt cccttaaaca cttcctctgt cagatctgat
     4741 aaccaccgga tctcattcgg ctgcatggca agatcaaaac cacctctgtt tcaagtccta
     4801 cctgtccact tcgattgtga ttacaattcc atctcccgca tcttcctgaa tcgcttcctg
     4861 cacaccctga atcacatctt cctgcagcgc atccttgtta gtagaagatt ttgcataaat
     4921 ttttatctcc ggattgccga gacttctcac atcatcccac gtaaattcag ccaggttgat
     4981 cccggtcggg ttgttcaaaa tctccagatc atccatatca cagtagattt ctgtcccgct
     5041 cagtgtggtc actgccccga agttctgggt gtcgccgttc tcatctacat acttatattg
     5101 tgtcgtcgta cggcgcatca gacgctcgat c
//
LOCUS       DBMDQJ010000078         5115 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6350_length_5115_cov_1.759872, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000078 DBMDQJ010000000
VERSION     DBMDQJ010000078.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6350_length_5115_cov_1.759872"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..766)
                     /locus_tag="ACZO9R_02500"
     CDS             complement(<1..766)
                     /locus_tag="ACZO9R_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0911976.1"
                     /translation="MEKRSYSYGMKITGVLLHSFFTIVLTVSVFLLASLVSKNILELT
                     DIGTDDFLSSGYYLKCMEQKCNDLNDYLLLTQKGALRTAEDEKRYLQYTNEFKQKSTN
                     FCYWYKINGAWYTNQPDTREGQQFDTQTVLMEAKTMGDYLLYDMEDKEFGTDIRGLEN
                     YFFSNNGNQIYWPLEDVVLVIGVDTQLSAEDDLRDAKIEYERLHPWIKVSIAGAIISV
                     VGWILTLVYLTLAAGHKEGEEGIALSSIDRIKTELMI"
     gene            complement(779..1471)
                     /locus_tag="ACZO9R_02505"
     CDS             complement(779..1471)
                     /locus_tag="ACZO9R_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007886172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0911977.1"
                     /translation="MQTILVCDDDKEIVEAIDIYLQQEGYQVLKAYDGEEALEILKEK
                     EVHLLIIDVMMPRLDGIRATLKIREESSIPIIILSAKTEDADKILGLNIGADDYVEKP
                     FNPLELVARVKSQLRRYTQLGNAAENDASVYTVGGLSINDDLKEVTVDGETVKLTPIE
                     YNILLLLVKNQGKVFSINQIYESIWNEDAIGADNTVAVHIRHIREKIEINPKEPRYLK
                     VVWGVGYKIDKN"
     gene            1699..2751
                     /locus_tag="ACZO9R_02510"
     CDS             1699..2751
                     /locus_tag="ACZO9R_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR0911978.1"
                     /translation="MKKKKTKKRRRVILLIAEIILILLLTLLLFAVVKLSKIQKTKLD
                     MDQVAINEDLSSETMDAMSDYRTVALFGLDNRTNNSLSKGRSDVIMLANINNKTHEVQ
                     LVSIYRDTYLDTGSGVFEKCNAAYAKGGPEQALSMLNTNLDLAITDYVTVDFSAIVEC
                     VDLLGGVEMTITDDEASLMLGYIQELNRLTGNTASCPSSGGTYTLNGVQATAFARIRY
                     GGGDDYKRTERQRAVLSAMIAKAQKSDLKTLNALINTLFSDIQTSFSNADLLALATNI
                     FNYKLSETNGFPYEKTAHRYDKVGDVVIPCGLSSNVTALHQFLYADMAYTPSDTVKNN
                     SEMITSKTGYREGDGF"
     gene            2977..3279
                     /locus_tag="ACZO9R_02515"
     CDS             2977..3279
                     /locus_tag="ACZO9R_02515"
                     /inference="COORDINATES: protein motif:HMM:NF024949.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0911979.1"
                     /translation="MADRLIELREKNHLSQSAVANRLGVTPALISAYEKTERNPSLER
                     LVALADIYHTSTDYILGRTTTDSYATIIEVKDLTDAQIRILRELVEVMKHTDATRS"
     gene            complement(3266..3637)
                     /locus_tag="ACZO9R_02520"
     CDS             complement(3266..3637)
                     /locus_tag="ACZO9R_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911980.1"
                     /translation="MTNIQRNNLYSLVLIGPAIFCILFYYFTGITPNIALSYLIIDMI
                     VIFAKRHFAIRRIREDQAYARLGDRTEVLLQMARELSEKDQMKREQNTDHTDKRNKNK
                     RHRRRGGTDKRGKRKSDTTTG"
     gene            complement(3829..>5115)
                     /locus_tag="ACZO9R_02525"
     CDS             complement(3829..>5115)
                     /locus_tag="ACZO9R_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0911981.1"
                     /translation="QLFKILDTMMASHIGASAVSAVAYLSQINMMISAVGTGLAVGAS
                     IKVSQAYGAGDFMLVKKRVSTMFAMCGALGIAILLVLVPFTPEFLRLMNTPEEFIADG
                     STYFRLELFGMVITFFNNVYIAIERARGNSKRILILNTAVIVVKLGLTAWFVYGLGGG
                     INMISCATIISQSLLLLAACINMNQKGNAFGFSLRAISIKKEVICPMLTLSFPVIVER
                     IAFAVGKVIVNSMSTVYGSWTVGALGISNNIGGITTNPQNGFQEGGSSIISQNLGAGR
                     PKRALLAFRWVLYIDMLIGLVIMTASLLCLNQLAGLFAGDNAEFAEMIKQIYRFEAVG
                     AIPLGVNAAVMALLYGFGKTRVTLVMNFCRVFLFRVPVLWALQQFTDLGNVSAGIVMA
                     VSNVASGVLAAVVGVIEIRRICNTYDVRYFGGERKN"
BASE COUNT         1300 a         1392 c         1091 g         1332 t
ORIGIN      
        1 caatcataag ttcggtcttg atgcggtcaa tggagcttaa ggcgatgcct tcctccccct
       61 ccttgtgtcc cgctgccagt gtcaggtaca caagcgtcag aatccagccc accacactga
      121 taattgcccc tgcaatgctc accttgatcc acggatgaag cctttcatat tctatcttgg
      181 catcccgcag atcatcctct gccgaaagct gtgtgtccac gccgatgaca agcaccacgt
      241 cttccagggg ccagtagatc tgattgccgt tattgctgaa aaaataattc tccagtcccc
      301 ggatatccgt gccgaattcc ttgtcttcca tatcgtaaag cagataatct cccatggtct
      361 ttgcctccat gagaaccgtc tgcgtatcga actgctgacc ctctctggta tccggctgat
      421 tagtatacca cgcgccattg atcttatacc agtaacaaaa atttgtactc ttctgcttaa
      481 attcattggt atactgcaga tagcgtttct catcctccgc tgtccgaagc gcgcctttct
      541 gcgtcagaag cagataatcg ttcagatcat tgcacttctg ctccatgcat ttcagataat
      601 acccggaact aaggaaatca tcggttccaa tatcggtaag ctccagaata ttcttgctga
      661 caagcgatgc cagaaggaac acggacactg taagaacgat tgtaaaaaag gaatgaagga
      721 gcactcccgt tattttcatt ccgtaactgt aggatctttt ctccataaag acgctcctct
      781 agttcttgtc aatcttatag cccacgcccc agactacctt gaggtagcgc ggctctttcg
      841 ggttgatctc gatcttctcg cggatatggc ggatatgaac cgccaccgta ttgtccgcac
      901 cgatcgcatc ctcgttccag atgctctcat agatctggtt gatggaaaat accttcccct
      961 gattcttcac aaggagaagc agaatgttgt attcaatcgg cgtcagctta accgtctccc
     1021 cgtccaccgt gacctctttt aaatcatcgt tgatcgacag accgccgacg gtatatacgg
     1081 acgcgtcgtt ctctgccgca ttgccaagct gcgtataacg gcgaagctgc gactttacac
     1141 gtgccaccag ttccagagga ttgaacggct tttccacata atcgtccgcg ccgatattaa
     1201 gtcctaaaat cttgtccgca tcttccgttt tcgcagatag aataatgatc gggatactgc
     1261 tctcctcccg gatctttaac gttgcccgga tgccgtccag ccgcggcatc atgacgtcga
     1321 tgatcagaag atggacttct ttttccttta agatctcaag cgcttcctct ccatcatacg
     1381 cctttaatac ctgatagcct tcctgctgta aatatatgtc gatcgcttcc acgatctctt
     1441 tgtcatcatc acataccaga attgtctgca ttctcttctc tccattctaa cgtggcagat
     1501 atcgcactgc atatcagcct ttctattgcc tgttttcttc attttaacag acacgcgcaa
     1561 attgttatag aggagatttc ttacttattt ttaaaaatct gcgcgcaaaa ttaagagaat
     1621 ctttattgcg ggcggcatat tcccgcttta agataagcag gtaacccaac catgctttta
     1681 ttctggagga tctttgatat gaaaaagaaa aaaacgaaga aacgccgcag agtcattttg
     1741 ctgattgcag aaattattct gattcttctg ctgactcttt tgttgtttgc agttgtaaaa
     1801 ctttcaaaaa tacagaaaac aaagcttgac atggatcagg tcgccatcaa tgaggatctg
     1861 tcctccgaaa caatggatgc catgtcagac tatcgcacag ttgctctctt cggtctggac
     1921 aaccgtacaa acaattccct gtccaaaggg cggagcgatg tcattatgct cgccaatatc
     1981 aacaacaaaa cgcacgaggt ccaactggtc tccatctacc gggataccta tctggacacc
     2041 ggcagcggag tctttgaaaa atgcaacgcc gcctacgcaa aaggaggacc cgagcaggcg
     2101 ctctcaatgc tcaataccaa tctcgatctt gcaatcacag attatgtgac ggtggatttc
     2161 agtgcaattg tcgaatgtgt ggatctgctg ggcggcgtgg aaatgacgat caccgatgac
     2221 gaagccagcc tgatgctcgg ctatattcag gaattaaacc gccttaccgg aaataccgct
     2281 tcctgcccgt cttccggcgg aacttacacc ttaaacggcg tgcaggcaac ggcatttgca
     2341 cgaatccgtt acggcggcgg tgatgattac aagcgcacgg aacgtcagcg tgcggtactt
     2401 tcagctatga ttgccaaagc acaaaaatcc gatctgaaga cgctcaacgc actaatcaac
     2461 actctcttca gcgatattca gaccagcttc tccaatgcgg atctgcttgc actcgccacc
     2521 aatattttta attacaagct cagcgagacc aacgggttcc catatgaaaa aaccgcccac
     2581 cgctatgaca aagtgggaga tgtcgtgatt ccctgcggcc tatcgtccaa tgtcacggca
     2641 cttcatcagt tcctctatgc agatatggct tacacgcctt ccgatacagt gaaaaacaac
     2701 agtgaaatga tcaccagcaa aaccggttac cgcgagggcg acggcttctg atcccgcttc
     2761 aaatgggctt tctgccatat cagtgacaac tgcgccttta atgataaaaa tacacctgct
     2821 atgatattct ttatcttcag cgggtgtatt ttttcatgtc atgcatattc taagtagtat
     2881 cattttcaac ttgcagttga actcttcata accgcaagta tgggaaaagt gcactagact
     2941 aagcaggaaa gcgaggcgtt gattatggac aagaatatgg cagaccgttt aattgaatta
     3001 cgtgaaaaga atcatctgtc ccagagtgca gtcgcaaatc gtctcggtgt aacgcctgcc
     3061 ttaatcagcg cttatgagaa aacggagcgt aaccccagtc tggagcgcct ggtcgcactg
     3121 gctgatatct atcacacatc cacggactat attcttggac gaaccacgac cgattcctac
     3181 gctacgatca tcgaggtaaa agacctcacc gatgcgcaga tccgtattct gcgtgaactt
     3241 gtcgaagtca tgaagcacac ggacgctacc cggtcgtagt gtcactcttt cttttgcctc
     3301 ttttgtctgt ccctcctctt ctcctgtgtc ttttattctt attcctttta tctgtgtgat
     3361 ctgtattctg ttccctcttc atctggtctt tttcacttag ttcccgcgcc atctgcaaaa
     3421 gaacctctgt gcggtcaccg agccgggcat acgcctgatc ctcccggatt cttcggattg
     3481 caaaatgcct cttggcaaag atgacgatca tgtcaatgat cagataagac agcgcaatat
     3541 tgggcgtaat ccctgtaaaa tagtaaaata agatgcaaaa aattgccggt ccgatcaata
     3601 ccagcgaata caggttgttg cgctgtatgt tcgtcataag ataaaaccag ctctcttcgg
     3661 gatccgcatc cacccagtgt gcgcgttcgt atgcaaatct ttcccgcctt ttcagaattc
     3721 tatctttaat actcatatcc tgtcctttat tctatcgaat tgatactgtt ttagacattt
     3781 taacatagag ttgaaatatt tgcaatctat aggtgtagtt tttatttctt aatttttccg
     3841 ttctccgcca aaataccgca catcgtatgt gttacagatt ctgcgtatct cgatgacgcc
     3901 caccaccgct gcaagcacgc cgctcgccac attgctcacc gccatgacaa tgccggcgct
     3961 gacgtttccg agatccgtga actgctgcaa cgcccaaagt accggcacgc ggaacagaaa
     4021 gacacggcag aagttcatga ccagcgtcac acgcgtcttt ccaaatccat agagcagcgc
     4081 catgaccgcc gcgttgacgc cgagcgggat tgctccgacc gcctcaaacc ggtaaatctg
     4141 tttgatcatc tcggcaaact ctgcattgtc cccggcaaag agacctgcca gctggtttaa
     4201 gcataacagc gacgccgtca tgatgaccag tccgatgagc atgtcaatgt aaagcaccca
     4261 gcggaacgcc aaaagcgcgc gcttcggtct gccagccccc agattctgac tgataatgga
     4321 ggagcctccc tcctgaaatc cgttctgcgg gtttgtcgtg attccgccga tattgttgga
     4381 gattccaagc gctcccaccg tccaggaacc gtagacggta ctcatcgaat tgacaatcac
     4441 ctttccaacc gcaaacgcaa tccgctccac gatgaccgga aacgacagcg tgagcatcgg
     4501 gcagatgacc tcctttttta tggagatggc acgcaatgag aagccaaacg catttccctt
     4561 ctggttcata ttgatgcacg cagcgagaag aagaaggctc tgcgagatga tcgtcgcaca
     4621 ggagatcata ttgatgcctc cgcccagccc gtagacaaac catgccgtca gccccagttt
     4681 cactacgatg accgccgtat tgagaatcag aatccgtttg gaatttcctc tggcacgctc
     4741 aatggcgatg tagacgttat tgaaaaacgt gatgaccatt ccgaacaact ccagcctgaa
     4801 atacgtgctc ccgtccgcaa taaattcttc cggtgtattc atcaggcgca ggaactccgg
     4861 cgtgaacggc accaggacaa gcagtattgc gataccgagc gccccgcaca tggcaaacat
     4921 tgtgctgaca cgctttttta ccagcatgaa atcgccggcc ccatatgcct ggctcacctt
     4981 gatgctggcg cccaccgcaa gcccggttcc caccgccgag atcatcatgt tgatctggga
     5041 caaatacgcg accgcggaca ccgcggatgc gccgatatgt gacgccatca tggtatcgag
     5101 tatcttaaaa agctg
//
LOCUS       DBMDQJ010000079         5090 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6375_length_5090_cov_1.693526, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000079 DBMDQJ010000000
VERSION     DBMDQJ010000079.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6375_length_5090_cov_1.693526"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            7..507
                     /gene="purE"
                     /locus_tag="ACZO9R_02530"
     CDS             7..507
                     /gene="purE"
                     /locus_tag="ACZO9R_02530"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR0911982.1"
                     /translation="MAKVGIVMGSDSDMPVMAKAADILEKLGIDYEMTIISAHREPDV
                     FFEYAKSAEAKGFKVIIAGAGMAAHLPGMCAAIFPMPVIGIPMHTTSLGGRDSLYSIV
                     QMPSGIPVATVAINGGANAGLLAAKILATSDDAILQKLKEYSKELKEQVQAKDARLQE
                     VGYQNY"
     gene            589..1614
                     /gene="purM"
                     /locus_tag="ACZO9R_02535"
     CDS             589..1614
                     /gene="purM"
                     /locus_tag="ACZO9R_02535"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR0911983.1"
                     /translation="MDYKNSGVDIEAGYKSVELMKKYVKGTMRPEVLGGLGGFSGAFS
                     MESFKNMEKPTLVSGTDGCGTKVKLAFLLDKHDTIGIDCVAMCVNDIACAGGEPLFFL
                     DYIACGKNYPEKIATIVSGVAEGCKQAGCALIGGETAEHPGLMPEDEYDLAGFSVGIV
                     DEKDIITGADLAAGDVLIGMASSGVHSNGFSLVRKIFSMDTDTLNTYHEELGKTLGEA
                     LLAPTRIYVKALREVKEVGVRVKACSHITGGGFYENIPRMLPEGKHAVIRKDSYEVPA
                     IFQMMAREGKVEEQMMYNTYNMGIGMIVAVDPADVDKTIAAMKAAGDTPYIVGEIKDG
                     EKGVTLC"
     gene            1608..2234
                     /gene="purN"
                     /locus_tag="ACZO9R_02540"
     CDS             1608..2234
                     /gene="purN"
                     /locus_tag="ACZO9R_02540"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR0911984.1"
                     /translation="MLKVAVLVSGGGTNLQAVLDAIDEGRITNARVDVVISNNPGAYA
                     LERAKGHGIEALCISPKDYESRAAFNEAFLAKLDSYQVDLVVLAGFLVVIPEQMIAQY
                     RNRIINIHPSLIPSFCGKGFYGLKVHEGALARGVKVTGATVHFVDEGTDTGPIILQKP
                     VAVEEGDTPEILQRRVMEQAEWIILPQAIDLIANGRVSVANGHVTVEK"
     gene            2269..3546
                     /gene="purD"
                     /locus_tag="ACZO9R_02545"
     CDS             2269..3546
                     /gene="purD"
                     /locus_tag="ACZO9R_02545"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR0911985.1"
                     /translation="MKVLVVGSGGREHAIVTSVAKSPRVDKIYCAPGNAGIASLAECV
                     AIGAMEFDKLVAFAKEHAVDFTIVGMDDPLVGGIVDVFEAEGLKVFGPRKNAAILEGS
                     KAFSKDLMKKYHIPTAAYENFTSAEDALTYLETAKMPIVLKADGLALGKGVLICNTLE
                     EAKAGVKEIMEDKKFGSAGNTMVVEEFMTGREVSVLSYVDGKTIKIMSSAQDHKRAKD
                     GDQGLNTGGMGNFSPSPFYTKEVDEFCQKQIYQATVDAMAAEGRPFTGVIFFGLMLTE
                     DGPKVLEYNARFGDPEAQVVLPRMKNDIIDVMEACVDGKLDTIDLQFEDNAAVCVVLA
                     SDGYPVSYEKGFPITGLEKFDGKDDYFCFHAGTKFDENGTIVTNGGRVLGITATGKDL
                     KEARAKAYEATEWVDFANKYMRHDIGRAIDEVK"
     gene            complement(3828..4658)
                     /locus_tag="ACZO9R_02550"
     CDS             complement(3828..4658)
                     /locus_tag="ACZO9R_02550"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005543 - phospholipid binding [Evidence
                     IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0006593 - L-ornithine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR0911986.1"
                     /translation="MKHFLRLTDYTKADIYEIFHITDEILEGHYQNLLQGKSVVLFFP
                     ESSIRTRVTFEKGIALLGGQPILFPSSALDKKEDLQDVIGYLNNWANLLVVRHRDMAA
                     LEKIAASASVPVINALTDANHPCEMAADLYALSKIRNDFTMDHFLFCGVTGNIGLAWK
                     EASEVMGFSLEQCCIPGYEMNGVPSHHCIRDAIRGKDIICTDSLPADLLSASRGCTVD
                     LAAMKLANEGAILNPCPPFYRGEEVSNDVIDSDYFVGYEFKKHLLTVQQAIMVYCLTH
                     "
BASE COUNT         1321 a         1053 c         1562 g         1154 t
ORIGIN      
        1 agcaatatgg caaaagtagg tattgtgatg ggcagtgatt ccgatatgcc ggtaatggca
       61 aaggctgccg acattttaga gaagcttgga attgactatg agatgacgat tatctcggca
      121 caccgtgagc cggatgtatt tttcgagtat gcaaagagcg cagaggcgaa gggctttaag
      181 gtcattatcg caggcgcggg catggcggcg catcttccgg gaatgtgcgc ggcgatcttc
      241 ccgatgccgg tcatcggtat cccgatgcac accacgtccc tcggcggacg cgactccctg
      301 tactccatcg tacagatgcc gtccggcatt ccggttgcca cagtggcgat caacggcggt
      361 gcaaatgcag gattacttgc agcaaagatt ctggcaacat cagatgatgc aattttgcaa
      421 aaattaaaag aatattccaa agaattaaaa gagcaggtgc aggcgaagga tgccagactg
      481 caggaagtgg gatatcagaa ttactaggaa caggaacgga tgattttgca aaaagtcgcc
      541 cgtgttgcgt tggatagaca acagacaagc atataggagg aaaataggat ggattacaag
      601 aattccggtg tggatatcga ggcaggatac aagtctgtag aactcatgaa aaagtatgta
      661 aagggaacca tgcgcccgga ggtgcttggc ggacttggcg gattttccgg tgcgttctcc
      721 atggagagct ttaaaaatat ggagaagcct acgttagtat ccggcacaga cggctgcgga
      781 acgaaggtaa aactggcatt tttactggac aagcatgaca cgatcggtat tgactgtgtt
      841 gccatgtgtg tgaacgatat tgcatgtgcc ggcggcgagc cgctgttttt ccttgactat
      901 attgcctgtg gcaagaacta tccggagaag attgcgacga tcgtgtccgg tgtggcggag
      961 ggttgcaagc aggcaggctg tgcgttgatc ggcggtgaga cggcagaaca tccggggctg
     1021 atgccggagg atgagtacga tctggctggt ttttccgttg gtatcgtgga tgagaaggac
     1081 attattacgg gagctgatct tgcggcgggc gatgttttga tcggtatggc aagctccggc
     1141 gttcattcca acggattttc cttggtcaga aagatctttt ccatggacac cgatacatta
     1201 aatacctatc atgaggagct tggcaaaaca cttggcgagg cgcttttagc tccgaccaga
     1261 atttacgtga aggcactccg tgaggtgaag gaagtgggcg tccgcgtaaa agcatgcagc
     1321 catatcacag gcggtggttt ctatgagaat atcccgcgta tgcttccgga gggaaagcat
     1381 gcggttatcc ggaaggacag ctacgaagtt ccggcgatct ttcagatgat ggcgcgcgag
     1441 ggcaaagtcg aggagcagat gatgtacaat acctacaata tgggtatcgg catgatcgtg
     1501 gcagtggatc cggcggacgt ggacaagacg atcgcggcaa tgaaggcggc aggagacaca
     1561 ccgtatatcg tcggcgagat caaggatggt gaaaaaggag taaccttatg ctaaaagtag
     1621 cggtactggt gtccggaggc ggcacgaatc tgcaggcagt cttagacgcc atcgatgagg
     1681 gcagaatcac aaacgcccgg gtagacgttg tgatcagcaa caatcccgga gcgtatgcgt
     1741 tggagcgcgc gaaagggcac ggcattgagg cactttgcat ttccccgaag gattatgaga
     1801 gccgtgcggc attcaatgaa gcgtttctgg cgaagcttga cagctatcag gtggatctgg
     1861 tggtgctggc gggatttctc gttgtaattc cggagcagat gatcgcgcag taccgcaacc
     1921 ggatcatcaa tattcatccg tcactgatcc cgtctttctg cggcaagggc ttttacgggc
     1981 tgaaggtgca cgagggagcg cttgcgcgcg gcgtgaaagt tacgggtgca acggttcatt
     2041 ttgtggatga gggaacggat acgggaccga tcattttgca gaagccggtg gcggtcgaag
     2101 agggagatac cccggagatt ttgcagcgcc gtgtgatgga gcaggcagag tggatcattc
     2161 ttccacaggc gatcgatctg attgcaaacg gcagagtgtc agttgcaaac ggacatgtga
     2221 cagttgagaa ataagcgtat gggtgacaga gtgcaggagg aagcggatat gaaggtattg
     2281 gtagttggaa gcggcggacg tgagcatgcg atcgtgacga gtgtggcaaa gagcccgcgc
     2341 gtggataaga tctactgtgc gccgggaaac gcgggaattg catcgcttgc agaatgtgtt
     2401 gcgatcggag cgatggagtt cgacaaactg gtggcgtttg cgaaggagca tgcagttgat
     2461 tttacaatcg tcggcatgga cgatccgctc gtcggcggaa ttgtggatgt gtttgaggcg
     2521 gagggcttaa aggtgttcgg accgcggaag aatgcggcga tccttgaggg ctcgaaggca
     2581 ttttccaagg atctgatgaa aaaatatcac attccgacgg ctgcctacga gaatttcaca
     2641 tctgcagagg atgcgcttac ttatctggag acggcgaaga tgccgatcgt attgaaggcg
     2701 gacggactgg cgctcggaaa gggtgttttg atctgcaata cgctcgagga ggcaaaggcg
     2761 ggtgttaagg agatcatgga ggacaagaag ttcggctccg cgggaaatac gatggtcgtg
     2821 gaggagttca tgaccggacg cgaggtatcg gtgctttcct atgtggacgg caagacgatc
     2881 aagatcatgt cctcagcgca ggatcacaag cgcgcaaagg acggcgatca gggactcaac
     2941 accggcggca tgggtaattt ttccccgagc ccgttctaca caaaggaagt ggacgagttc
     3001 tgccaaaagc aaatctatca ggcaaccgtg gatgcgatgg cggcagaggg aagaccgttt
     3061 accggcgtta ttttctttgg actgatgctg acggaggacg gaccgaaggt gcttgagtac
     3121 aatgcgcgtt ttggcgatcc ggaggcacag gtggtgctcc cacgcatgaa gaatgacatc
     3181 attgatgtga tggaggcatg cgtcgatgga aagctggata cgatcgacct tcagtttgaa
     3241 gacaatgcgg cggtatgtgt ggtgcttgca tccgacggtt atccggtgtc ctatgagaag
     3301 ggatttccga tcacaggact tgaaaagttc gacggaaagg acgattattt ctgcttccat
     3361 gcgggaacga agttcgatga gaacggcacg atcgtcacga acggcgggcg tgtgctcggc
     3421 attacggcga ctggaaagga tttgaaggag gcgcgcgcaa aagcatacga ggcgacagag
     3481 tgggttgatt ttgcgaataa gtacatgcgc catgacatcg gacgtgcgat cgacgaggta
     3541 aagtagaata aaaagaaaca ggaagctccc gttcgtggga tgccggtatt ttctacagag
     3601 tgctgtggaa aagagaggta tcacacgcac gggagttttt ttattcatag gaaattacgt
     3661 cctatgaatg aaaaagccct ccgggcagga tcgcactgcg tcggagtgga attatatcgc
     3721 tagagcaacc cgccgcaggc ggagaatcct gcaaaacagg attttttctt gcagaaaata
     3781 ttgtgttatg tcaaaaaatg aaaagcttaa tgcgttatat gaaaagttta gtgtgttaag
     3841 cagtacacca tgattgcctg ctggacggtg agcaggtgct ttttaaattc atatccgaca
     3901 aaatagtcgg agtcgatgac gtcgtttgag acttcctcgc cgcggtaaaa aggcgggcag
     3961 ggatttaaga tggcaccctc gtttgccagc ttcatggcag caagatcgac ggtgcagccg
     4021 cgggaggcgg aaagaaggtc ggccggcagt gagtcggtgc agatgatgtc ttttccacgg
     4081 atggcgtcgc ggatgcagtg atgtgagggc acgccattca tctcatatcc cggaatacag
     4141 cactgctcca gcgaaaagcc catcacttcg gaggcttcct tccaagccag cccgatattt
     4201 cctgtgacac cgcagaacag aaagtggtcc atggtaaaat cattccgtat ttttgagagc
     4261 gcatacagat ctgcagccat ttcgcaggga tgatttgcgt cggtcagcgc attgatgaca
     4321 ggtacggatg cagatgcagc gattttttcc aaagcggcca tatcccggtg gcggacgaca
     4381 agcagattgg cccagttgtt gaggtagccg atgacgtctt gcaaatcttc ctttttgtcg
     4441 agagcggaac ttggaaacaa aatcggctga ccgcccaaaa gcgcgatgcc cttctcaaac
     4501 gtgacccttg tgcggatgct ggattccggg aaaaataaaa cgacgctttt gccctgcagc
     4561 agattctgat agtgcccctc caggatctca tcggtgatat ggaagatttc gtaaatgtca
     4621 gctttggtat agtcggtcaa tcggagaaaa tgtttcaatg gaaccgcctt tctggctgcc
     4681 ggtgcggtag tgcggcagac ctgtgctaaa tgtcagaaaa atcaatgggt tgcgctctta
     4741 tcgtatcatt tttgcgggtt agaaggaagc tataatatta gaagaaagct atatttttgt
     4801 gggatatgct ttagaaacag tgctaaagat gttttgcggg tctggagtgg agaaaaggtg
     4861 cgctcagacc catgagttgg aattatgtta acttatttga aatgtttttg tgcgacaccg
     4921 ggtctgtaga tgtgaaaata agcagctaga cccgggaaat ggaaattatg tcaaccattt
     4981 tggatagtca agcccggaaa cagggtctgg ggagagaaaa atacgcttcc agacccgccc
     5041 aaggaagaaa accaggtcta gggaatgtga aaacaagcaa ttagacccgg
//
LOCUS       DBMDQJ010000080         5051 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6426_length_5051_cov_1.694747, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000080 DBMDQJ010000000
VERSION     DBMDQJ010000080.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5051)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5051)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5051
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6426_length_5051_cov_1.694747"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="ACZO9R_02555"
     CDS             complement(<1..344)
                     /locus_tag="ACZO9R_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HIR0911987.1"
                     /translation="MEEKALRLSAVFLMILTAVSFVLLPKLPALHTWEVEAREERIAE
                     EEFAQSQVTMQNLKIKEQNEAAGAKGELSIRLPEGVTGDQIAVSNDYVTQTIRVEIPQ
                     ADRTYFDSYPVLG"
     gene            complement(359..2065)
                     /locus_tag="ACZO9R_02560"
     CDS             complement(359..2065)
                     /locus_tag="ACZO9R_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin like domain-containing protein"
                     /protein_id="HIR0911988.1"
                     /translation="MKYSKRYIVFGVLVAAMLIFKLEPGASGSGTVENFRGANLQATT
                     WNPLIASSVNDNLLSLVIDNKEYTNENHTFYMDDQLNIMVPVNILRDALNCSAHVYDG
                     KRLLVEKHANEVEFSLGASEAKVNGTREVIVSPFIRKDDTYYVSLNDLANYLDYSYSW
                     DIQENQASAMDTSDSATIIPTRYDLREKGRAAAVRNQGTFGTCWAFAALGALESSLLP
                     EEKLQFSVDHMTMNNGFTLTQDDGGEYTMGMAYLAAWEGPVYEADDPYGDNKTNPDLT
                     AVKHVQEMQIIDGKDYEKIKESVFLYGGVQTSIYNGLRSSQSKSPYYNQKTNSYCYIG
                     TEKPNHDVVIIGWDDSYSKENFNVDLEGDGAFICQNSWGDTFGEDGVFYISYYDTNIG
                     THNVVYTKIEEPGNYDHIYQSDLCGWVGQLGYNKDSAYGANVYTTSQEEELVAAGFYA
                     TGKDSQYELYVVRNFENADSLTQRIPVASGKLSNAGYYTVDFNQAISLDAGERYAIVL
                     HMITPGSVHPMAIEYAADEATANVILDDGESYISTNGARWDSVDTVEECNLCIKAFSK
                     NR"
     gene            complement(2062..3234)
                     /locus_tag="ACZO9R_02565"
     CDS             complement(2062..3234)
                     /locus_tag="ACZO9R_02565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing class I SAM-dependent
                     RNA methyltransferase"
                     /protein_id="HIR0911989.1"
                     /translation="MKTYELVVPCHFGLEAVLKREIYDLGYEISRVEDGRVSFQGDAE
                     AVCRANIFLRTAERVLIEVGRFRATTFEELFQGIKALPWEDYIPVNGKFWVKKASSIK
                     SKLFSPSDIQSIAKKAMVERMKQYYDVEWFAEDGAPYPVRIFLLKDEVMVTLDTSGDS
                     LHKRGYRTLTSKAPLTETLAASLLLLTPWRPDRILVDPFCGSGTFPIEAAMIAAGIAP
                     GMNREFTAEQWTNLIDRKLWYECVKEAEEMIHTDVKVDIQGYDIDGDVIKAARENAKR
                     AGVDHLIHFQQRPVAELHHPKKYGFIVTNPPYGERLEDKADLPKLYGEIGAAFRGLDS
                     WSMYLITSYDEAERYIGRKADKNRKIYNGMLRTYFYQFMGPKPPRRSVRDTAGGDE"
     gene            complement(3299..3595)
                     /locus_tag="ACZO9R_02570"
     CDS             complement(3299..3595)
                     /locus_tag="ACZO9R_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR0911990.1"
                     /translation="MTFYTIHPRELEDIMRRKHGILIDVREADSYREYHYPNARNLPY
                     EKIDQWMHRLPKNRPLVLYCDYGSTSLLAARKLGREGYEVYTVVGGIEAMRTSD"
     gene            complement(3723..3905)
                     /locus_tag="ACZO9R_02575"
     CDS             complement(3723..3905)
                     /locus_tag="ACZO9R_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911991.1"
                     /translation="MAKNESTQNSTSNCRDGYENSYENNAGSGSNSAKNKSTNKSTNK
                     TSNVSGSNKTSDKYQQ"
     gene            complement(4162..>5051)
                     /locus_tag="ACZO9R_02580"
     CDS             complement(4162..>5051)
                     /locus_tag="ACZO9R_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR0911992.1"
                     /translation="FMSNRELKVKFEIQNMFTSGNRATYGKLSTFCPILGEYDLINSI
                     EKMLVTAQKLDESLHKIRDIDFSAFYREVQFSDPDKGINREMIMKEIMPDIILMPNAG
                     TRSMMWQETAGVRRDTSARFLFPIFTAVDIDDMMVDTVGRYRWEICRKIQGVHWNDIR
                     EKSLTAEYCDYLQFYRKNRELSTEAKEKIKSALLRGRNNYREVFVKDYQNWMKYESKG
                     SYRLNKIAREILITYCPFAKPIREELKGNPMYESALHRLDLLNAKTVQRIQGVYDKYQ
                     KAGGRITQELTDNLEYYEL"
BASE COUNT         1099 a         1489 c         1068 g         1395 t
ORIGIN      
        1 cccagaaccg ggtagctgtc aaaataagtg cggtccgcct gcgggatttc cacccggata
       61 gtctgggtca cataatcgtt gctgacagca atctgatccc cggtcacgcc ctccggcagg
      121 cggatactca gttctccctt cgcccccgcc gcttcattct gctctttgat ctttaagttc
      181 tgcatcgtga cctgactctg ggcgaactcc tcctcggcaa tgcgttcctc tctcgcctcc
      241 acctcccacg tgtgaagcgc cggaagcttc ggcagaagca caaaagatac cgctgtcaaa
      301 atcatgagaa agacagccga cagtctcaat gctttttctt ccataaccgt atattccttt
      361 atcgattctt actgaatgcc ttaatacaga gattacattc ctccaccgta tccacgctgt
      421 cccatctggc accattggta ctgatataac tctcaccatc gtccagaatc acattcgcgg
      481 tcgcctcgtc ggcagcgtat tcgattgcca tcggatgcac cgaccccggc gtaatcatgt
      541 gaagcacaat cgcataacgc tcccccgcat ccagtgaaat tgcctgatta aaatcaaccg
      601 tataatagcc cgcgttggag agcttgccgg acgccaccgg aatccgctgt gtcagcgaat
      661 ctgcattttc aaaattccgt acaacataca gctcgtactg ggaatctttt cctgttgcgt
      721 aaaagcctgc cgccaccagt tcttcttcct gtgacgtcgt gtagacattg gcgccgtagg
      781 cgctgtcctt gttatagcca agctgtccga cccagccgca cagatcgctc tgatagatat
      841 ggtcatagtt gcccggctct tcgatctttg tatacacaac gttgtgggtg cctatattcg
      901 tatcataata cgatatgtaa aacaccccgt cctcgccgaa cgtatctccc cagctgttct
      961 ggcagataaa tgcgccgtct ccctcgagat ccacattaaa attctccttg gaataactgt
     1021 cgtcccagcc gataatcaca acgtcgtgat tcggcttctc tgtaccgatg tagcagtagg
     1081 aatttgtctt ctgattgtaa tacggagact ttgactgcga gctgcgcagc ccgttataga
     1141 tcgacgtctg cactccgccg tataaaaaga ccgactcctt gatcttctca taatcttttc
     1201 cgtcgatgat ctgcatttcc tgcacatgct tgaccgctgt aagatccgga ttcgttttat
     1261 tgtccccgta agggtcatcc gcttcgtata caggtccttc ccatgccgcc agataagcca
     1321 ttcccatggt atattccccg ccgtcatcct gcgtcagtgt aaatccattg ttcatcgtca
     1381 tatggtcgac cgaaaactgc agtttctctt ccggcagcaa cgacgactcc agtgccccca
     1441 gcgcggcaaa cgcccagcag gtgccgaaag ttccctgatt gcgcactgct gccgcccgtc
     1501 ccttctccct cagatcatag cgggtcggta tgattgtcgc actgtccgac gtgtccatcg
     1561 ccgacgcctg attctcctgt atatcccagg aataggaata atccagataa ttcgccagat
     1621 cattcaggct tacatagtag gtatcgtcct tgcggataaa cggcgacacg atcacctcac
     1681 gggttccgtt gactttcgcc tccgaggcgc ccagtgaaaa ctccacctca ttcgcatgct
     1741 tctccacaag gagccgcttt ccgtcataca catgcgcgct gcagttgagt gcatcgcgca
     1801 gaatgttcac cggaaccatg atattcagct ggtcatccat ataaaacgta tggttctcgt
     1861 tggtgtattc cttgttatcg atcaccagcg acaacagatt atcatttacg ctggatgcga
     1921 tcaacgggtt ccaggttgtc gcctgcagat tggctccgcg gaaattttcc accgttccgc
     1981 tccccgatgc ccccggttct aatttaaaaa tcagcatggc ggctaccagc acgccaaaca
     2041 cgatatatct ttttgaatat ttcattcatc ccctccggcg gtatccctta cactccgcct
     2101 tggtggcttc ggccccataa actggtaaaa ataggtgcga agcattccat tgtagatctt
     2161 gcggttcttg tccgcttttc tgccgatata gcgctccgcc tcatcataac tcgtaatcag
     2221 gtacatcgac caggaatcaa gtccccggaa tgccgccccg atctccccgt acagcttcgg
     2281 cagatccgcc ttgtcctcga ggcgctcgcc atacggcgga ttcgtcacga taaagccgta
     2341 tttcttcggg tggtggagtt ctgccaccgg gcgctgctga aaatgaatca ggtgatccac
     2401 gccggcgcgt tttgcattct ccctggctgc ctttatgacg tccccgtcaa tatcatagcc
     2461 ctgtatgtcg accttcacat ccgtgtggat catctcctcg gcttccttta cgcactcgta
     2521 ccacagcttg cggtcaatca ggttcgtcca ttgctccgcc gtgaactcac ggttcattcc
     2581 gggcgcaata ccggcagcaa tcatcgccgc ctctatcggg aacgtaccgc tgccgcagaa
     2641 cggatccacc agaatccggt ccggtctcca cggcgtcaac agcagcaggg aagctgccag
     2701 cgtctcggtc agaggcgcct tgctcgtcaa tgtgcggtat ccgcgcttgt gcagagagtc
     2761 cccggacgta tccagcgtca ccataacctc atccttcagt agaaagatgc gcaccggata
     2821 cggcgccccg tcctccgcga accattccac atcatagtac tgcttcatgc gctccaccat
     2881 cgccttcttc gcgatcgact gaatatcaga cggagaaaac agcttgctct tgatagaaga
     2941 cgcctttttt acccagaact ttccgttcac agggatatag tcctcccacg gaagcgcctt
     3001 gattccctga aataattcct caaaggtcgt tgcgcggaat ctgcccactt cgatcagcac
     3061 gcgttctgcc gtgcgcagaa agatattggc acggcagaca gcttccgcat ccccctgaaa
     3121 tgagactctg ccatcctcca cccggctgat ctcatatccc aggtcataga tttctctctt
     3181 aagtaccgct tccagtccaa aatgacatgg aaccaccagt tcatatgtct tcatgtattc
     3241 tcctctgtat catatgtcta atttcctaat aatatattat ccattctaat tttccctgtc
     3301 aatcacttgt tcgcattgcc tcaatgccgc caaccacggt atatacctca tacccctccc
     3361 tgccgagctt tctcgccgcc agaagactgg tgcttccata atcgcagtag agcaccagcg
     3421 gtctgttctt cggcagacgg tgcatccact gatcgatctt ttcatacgga agatttctgg
     3481 cgttcggata atggtattcc cgataagaat ccgcctcccg cacatcaatc agaattccgt
     3541 gctttcttct cataatatct tccaattccc ttggatgtat cgtataaaac gtcatgcccg
     3601 cgtatcctcc ttttccgcca tatgctcctg taatcgcaaa aacagattac aaagaggttc
     3661 tctgtaatct gtttctgtca atcttattct atcctatgcg ctttttctct gcctgtgcca
     3721 gtctactgct gatacttgtc agaggtcttg ttggaaccgg acacgttgga tgtcttgttg
     3781 gttgacttgt tagtcgactt attctttgcg ctgttggaac cgcttcccgc gttgttctcg
     3841 tagctgttct cataaccgtc tctgcaatta gaagtcgaat tctgtgtact ttcatttttt
     3901 gccatgttgc cctccatccc ggcacgtgct cgtgccgaat ctttttattt gcttactgca
     3961 tggttagtat ctgatgttcc ccggaaaata tacatggctt ttgtggtttt gcattgcttt
     4021 ttcaaaaaga atgttctata ctaatgctgt gatatgtaaa acatatcatt ttatacccct
     4081 tattaattat ttatactccc ctcagaaaaa gccaagtgca tcgcacctgg ctttttctat
     4141 cttatcacag ttcatatctt atcacagttc ataatattcg agattgtcgg taagctcctg
     4201 cgtgatcctg cctccggctt tctgatactt gtcatagact ccctggatac gctgcacggt
     4261 cttcgcgttc aaaagatcga ggcggtgcag agcgctctca tacatcggat tcccctttaa
     4321 ctcctcccgg atcggctttg cgaacgggca ataggtaatc aggatctctc ttgcgatctt
     4381 gttgaggcgg tagcttccct tcgactcata cttcatccag ttctgataat ccttgacgaa
     4441 gacctcacgg taattatttc ttccacgcaa gagcgcactc ttgatcttct ccttcgcctc
     4501 ggtggaaagc tcgcggttct tccggtagaa ctgcagataa tcgcaatact ccgccgtcag
     4561 ggacttctca cggatatcgt tccagtgtac accctgaatc ttcctgcaga tctcccagcg
     4621 gtatcttccg accgtatcga ccatcatatc gtcaatatcg accgctgtga aaatcgggaa
     4681 caggaaacgc gccgaggtat ctcttcgcac gcctgctgtc tcctgccaca tcatcgagcg
     4741 tgttcctgca ttcggcatca gaatgatgtc cggcatgatc tccttcataa tcatctcgcg
     4801 gttgatgccc ttgtccggat ccgagaactg cacttcacga tagaacgcgg agaaatcgat
     4861 gtcgcggatc ttgtgcagtg actcgtccag cttctgcgcc gtcacgagca tcttttcaat
     4921 ggagttgatc agatcatact cccctaaaat cgggcagaac gtggacagct tgccataggt
     4981 cgcacggttt ccggacgtga acatgttctg aatctcaaac ttgactttga gctcccggtt
     5041 ggacataaaa t
//
LOCUS       DBMDQJ010000081         5027 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6463_length_5027_cov_1.370889, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000081 DBMDQJ010000000
VERSION     DBMDQJ010000081.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5027)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5027)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5027
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6463_length_5027_cov_1.370889"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /locus_tag="ACZO9R_02585"
     CDS             <1..1251
                     /locus_tag="ACZO9R_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HIR0911993.1"
                     /translation="ERVPWAEHGYYYTAQDTPGKHPYHEAGVYYIQEPSAMAPAAYLT
                     RQMADGAGERILDLCAAPGGKSTQIAAAMAGRGILISNEIHPARAKILSENIERMGIA
                     NAVVTNETPQRLAEHFSEYFTRIMVDAPCSGEGMFRKNEEACEQWSTQNVELCADRQD
                     EILDCAASMLAGGGRLVYSTCTFAPAEDEGSIGRFLERHPEFYVEEVPLAEGMSHGLG
                     ELSATIRLWPHKLRGEGHYVAVLRKEGTLADGGSGLLKNGYENGLAAGDSKSAAAGIP
                     EYLAFAGETFAGDALEKYAGRECRYLKFGEQLYRIPEGMPSVKGLKVLRPGLHLGTAK
                     KGRFEPSHALALTLHAADVLHTMDLPSDGREIRGYLNGETFPAQGEKGWYLITVDGYS
                     TGWGKLAGGIMKNHYPKGLRKRLP"
     gene            1400..>1797
                     /locus_tag="ACZO9R_02590"
     CDS             1400..>1797
                     /locus_tag="ACZO9R_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR0911994.1"
                     /translation="MKITTRKVAIVGCGLVGSTTAFSLVTQGVCDEVMLIDINEERAY
                     GEMLDMRDSIEYLNRNVKVKTGTYAECGDMDIIVITAGAPPKQGQTRLDTLEMSAKIA
                     KSIVEPIMKSGFDGIFIAISNPVDIIAHYI"
     assembly_gap    1798..1807
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1808..2372
                     /locus_tag="ACZO9R_02595"
     CDS             <1808..2372
                     /locus_tag="ACZO9R_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR0911995.1"
                     /translation="YKLSGLPKNQVIGTGTAIDSARLQNMIAQTVNVDPRSVHAYSMG
                     EHGDSQMVPWSTVTIAGKPFYDVIADNKELVGDVDLDELVYRTTQEGWEIFNRKGTTY
                     YGIATACVGVIKAILNDENRIIPVSTLLEGEYGERDVYAGVPTVLNRSGAYDVLEIHM
                     TPGELAHFKESTRVIREYTDRIMKLNL"
     gene            2441..3046
                     /locus_tag="ACZO9R_02600"
     CDS             2441..3046
                     /locus_tag="ACZO9R_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0911996.1"
                     /translation="MERRYIESRLWTCLTSAFLEQVRSRYHFEEKDKALLKGVASDLA
                     GCLKWKEAAYLMFEDGTDTVRRVTAVVTLGSDVDLLQERYQNTGRMLESYMLESIGGE
                     LLMLGYGMLEAWIRENTGYQVAAYHFFGDDEAHPLSAMPEILARAKQHKVVCTAGYCL
                     KPKKSVVFEMELAPAEACGETAHGGRICAHCSRRDTCPNRR"
     gene            3151..4752
                     /locus_tag="ACZO9R_02605"
     CDS             3151..4752
                     /locus_tag="ACZO9R_02605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR0911997.1"
                     /translation="MGDAKSIKERGILEQVIKSELDFITYVDVHTGTFHVIVTNDDTD
                     VTPPPEGDYTQVNAQMIPLYVHPKDREACAAALELPHILSELEQRDDLVVSYRLLCGE
                     VYRRKELHISYQQDDRDTVVLVRRDVTESYEEEQRQQERLMSALFDARHANQEKNEFL
                     ERMSHEIRTPMNSIIGLTYLTREYVNNEKQVLENLDKISQSARFMLSFVDDILNLSQI
                     ESGNVALNSQKVVLDAFLEETVEEAMRQAAERRVHFAVDRRGSFDATYCFDAEKLERA
                     LSNILENAVKYTMPDGRVDFIVEHTGDDGEDAGFRFEIRDTGVGMDEAFIPHMFEPFE
                     QEDEGITTLNGGTGLGLPIARNIIEFMGGHIDAYSEKGKGSTFIVNVNLKRVRNSGEK
                     LRGQGNVQAPDYDFTGKRALLVEDNEVNIEITRNILLHKNLQVDVAVNGEEGVTHFLS
                     HEPGYYDVILMDIRMPVMDGLTATRKIRESGREDGKTVPIVAMTANVFEEDVRKSLDA
                     GMDAHLSKPIEITQMYAVLDSMIYR"
BASE COUNT         1338 a         1009 c         1594 g         1076 t
ORIGIN      
        1 gagcgtgtac cgtgggcaga gcatggctac tattacacgg cgcaggatac gccgggaaaa
       61 catccgtatc acgaagccgg cgtatattat atccaggagc cgagcgccat ggctccggca
      121 gcgtatctga cgcggcagat ggcagacggt gcgggcgagc ggattcttga tttgtgcgcg
      181 gcaccgggag gaaaaagcac gcagattgcg gcggcgatgg caggcagggg aatcctcatc
      241 agcaatgaga tccatccggc gagggcaaag attctctcgg agaatatcga gcgcatggga
      301 attgccaatg cggtggtgac gaatgagacg ccgcagcgcc tcgcagaaca tttcagtgag
      361 tattttacca gaatcatggt ggacgcaccg tgttccggcg aggggatgtt ccggaagaac
      421 gaggaggcgt gtgagcagtg gagcacacag aatgtagaac tgtgtgccga cagacaggat
      481 gagatcttag actgcgcggc atccatgctt gcgggcggcg ggagactggt ctattcgacc
      541 tgcacatttg caccggcgga agacgaggga agtatcgggc gattcctgga gagacatccg
      601 gaattctacg tggaggaggt gccgcttgcg gagggaatgt cgcacggact gggagaactt
      661 tccgcgacga tccgtctgtg gccgcataag cttcgcgggg aaggacatta tgtggcggtg
      721 ctgcgcaagg agggaacgct tgcggacggc ggatcgggtc tgttaaagaa cgggtatgag
      781 aacggacttg cggcaggaga ttctaagagc gctgcggcgg gcatcccgga atatctggca
      841 tttgccgggg agactttcgc cggggacgca ttggagaagt atgcaggacg ggagtgccgc
      901 tatctgaaat tcggcgagca gctctaccgg atcccggagg gcatgccgtc ggtaaaaggt
      961 cttaaggtgc tgcgcccggg actgcatctg ggaacggcga agaaaggcag atttgagcct
     1021 tcacacgcac ttgcgctcac acttcatgcg gcggatgttt tacatacgat ggatcttcct
     1081 tcggacgggc gggagatccg tggataccta aacggggaga cgttcccggc gcagggagaa
     1141 aagggctggt atttaatcac ggtggacgga tacagtaccg gctggggaaa gctcgcgggc
     1201 ggaatcatga agaatcatta cccgaaggga ctgcgaaaac ggcttccctg atagaattgt
     1261 ctttgtggag aaaaagagag gacttgacaa gagagaaaac gctttctata attgaaaaag
     1321 gatttgcaag ggtgctgtca taggacggta ccttttttgc gtcataggat gaaaatccgt
     1381 tgaaaggaca ggtgaatgta tgaaaataac aacaagaaaa gtagcaattg tcggctgtgg
     1441 actggtgggc agcactacgg cattttccct ggtaacccag ggagtatgtg acgaggtgat
     1501 gctgatagat atcaacgagg aacgcgcgta tggagagatg ctggacatgc gggacagcat
     1561 tgaatatctg aaccgcaatg tgaaagtgaa aacgggaacc tatgcagagt gcggagatat
     1621 ggatatcatt gtgatcactg ccggagcgcc gccaaagcag ggacagacga gactggatac
     1681 gctggagatg tcggcaaaaa tcgcaaaatc cattgttgaa ccgattatga aaagtggatt
     1741 tgacggaatt tttattgcga tctccaatcc ggtggacatt attgcacatt atatctannn
     1801 nnnnnnncta taagctgtcc ggtttgccga aaaatcaggt gatcggtacc ggaaccgcaa
     1861 tagactcggc ccgtttgcag aatatgattg cacagacagt caatgtggat ccgcgaagtg
     1921 ttcacgcgta ttccatgggg gaacatggag attcccagat ggtgccgtgg tctacggtta
     1981 cgatcgcagg aaaaccgttt tatgacgtga ttgcggacaa taaagagctg gtgggggatg
     2041 tggatctgga tgaactggtc taccgcacca cacaggaagg gtgggagatt tttaaccgca
     2101 agggaacgac ttattatgga atcgcaacgg catgcgtggg agtgatcaag gcgattttaa
     2161 atgatgagaa caggatcatt ccggtatcaa cattgctgga aggagagtac ggggagcggg
     2221 atgtctatgc gggggtgcca accgtgttaa accgcagtgg ggcatatgac gtgcttgaaa
     2281 ttcatatgac accgggtgaa ctcgcgcact ttaaagaatc caccagagtg atccgcgaat
     2341 atacggatcg cattatgaaa ttgaatttgt aaaggaaaga agaaaggaat tgcaatctcc
     2401 ctgtaatgca gtacaataag agcaggaaag ggagcgcagt gtggaacgaa gatacataga
     2461 gagccggctc tggacctgtc tgacaagtgc gtttttagaa caggtcagga gccggtatca
     2521 ttttgaggaa aaagataagg cgcttttgaa gggtgtggca tccgatctgg cgggctgcct
     2581 gaaatggaag gaagcggcgt atctgatgtt tgaggatggg acggatacgg tacgccgggt
     2641 gactgcggtt gtgacgcttg gaagcgatgt ggacctgctg caggaacgct accagaacac
     2701 cggacgcatg ctggaaagct atatgcttga gagtatcggc ggagaacttc tgatgttagg
     2761 ctatggcatg ctggaggcat ggatacggga gaataccggg tatcaggtgg cggcgtatca
     2821 ttttttcggt gatgacgaag cacatccgct gtctgcgatg ccggagatct tagcgcgggc
     2881 aaaacagcac aaggtcgtct gtacggcagg gtattgcctg aaaccgaaga aaagcgtggt
     2941 gtttgagatg gagcttgcgc cggcggaggc gtgcggggag acggcacatg gcggcagaat
     3001 ctgcgcacat tgcagcagga gggacacatg cccgaaccgt aggtagaact gcactttcag
     3061 ggggaaaaat gaaaaattgc agtggaaagt ttttcaaatc ctgttataat aaagcataat
     3121 ttgttttttt gaaataaagg agtatgcaga atgggagacg cgaaatccat caaagagcgc
     3181 ggaatactgg agcaggtcat caagtcggaa ctcgatttta ttacctatgt ggatgtacat
     3241 accggaacat ttcatgtgat cgtgaccaat gacgatacgg atgtgactcc gccgccggag
     3301 ggagattata cacaggtaaa tgcgcagatg ataccgctgt atgttcatcc caaggaccgg
     3361 gaggcatgcg cggctgcgtt agaactgccg catattctct cggaactgga acagagggat
     3421 gatctggttg tttcgtaccg cctgctgtgc ggggaggtat accgcagaaa agaactgcat
     3481 atttcttatc agcaggatga ccgtgacacg gtggttttgg tgcgccggga cgtgacggag
     3541 agctatgagg aagaacagcg gcagcaggag agactgatgt cggcgctgtt tgacgcaaga
     3601 catgccaatc aggagaagaa cgagtttttg gagcgcatga gtcacgagat ccggacgccg
     3661 atgaattcca tcataggtct tacgtatctg accagggaat atgtgaacaa tgagaagcag
     3721 gtgctcgaga atctggacaa gatcagccag tcagcccgtt ttatgctgtc ttttgtggat
     3781 gatattttaa atctttccca gatcgagagc ggcaacgtcg cattgaacag ccagaaggtg
     3841 gtcttagatg cctttttgga ggagacggtg gaagaggcga tgcggcaggc ggcggagcgt
     3901 cgcgttcact ttgcggtgga cagacgcgga agctttgatg ctacctattg ctttgacgcg
     3961 gagaagctgg agcgggcgct ttccaatatt ctggagaatg ccgtaaaata cacgatgccg
     4021 gacggaaggg ttgactttat tgtggagcat accggcgatg acggggagga tgccggcttc
     4081 cggtttgaga tccgggatac gggagtcgga atggacgaag cgtttattcc gcatatgttt
     4141 gagccgtttg agcaggagga tgaaggaatc acgacgctaa acggcggtac cggtcttggt
     4201 cttcccattg cgcgcaacat tattgaattt atgggcgggc acattgacgc atacagcgaa
     4261 aagggcaagg gctcgacgtt cattgtgaat gtgaacttaa agcgtgtcag aaattccgga
     4321 gaaaagctgc ggggacaggg caatgtacag gcgcccgact acgattttac cggcaaaaga
     4381 gcgctgctgg tcgaggacaa tgaggtcaac attgagatca cgcgcaatat cctgctgcac
     4441 aagaatctgc aggtggatgt ggcggtcaac ggagaggagg gcgtgacgca ttttctttcc
     4501 catgagcccg gatattacga cgtcatcctg atggatatcc ggatgccggt catggacgga
     4561 ctgacggcga ccagaaagat ccgggaatcc ggacgtgagg atggcaaaac ggttccgatc
     4621 gtggcgatga cagccaatgt gtttgaggag gacgtgcgaa agtccctcga tgccgggatg
     4681 gatgcacact taagcaagcc gattgagatt acacagatgt atgccgtcct cgacagtatg
     4741 atttaccgtt gagaagaaaa aggggctggt gctttaagca ccagcccctt tttatgtcga
     4801 attttgtaga aggggaagga caggggcgtt ggatgttaaa aaacggtacg gattttgctg
     4861 agaaataaaa gatggtatgt tatattttaa aagatatgtt accgatacga taaaagaatg
     4921 gagccgaatc aatgcaaaag aacagagtga tgctggtagc ggatgatatc gacgtaaaca
     4981 gagcgtcgtt tcgcgcaatg tttgaagagg aatatgaggt gctggaa
//
LOCUS       DBMDQJ010000082         5009 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6490_length_5009_cov_1.419112, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000082 DBMDQJ010000000
VERSION     DBMDQJ010000082.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6490_length_5009_cov_1.419112"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            239..1924
                     /locus_tag="ACZO9R_02610"
     CDS             239..1924
                     /locus_tag="ACZO9R_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0911998.1"
                     /translation="MRERRWIQKGYWIFFAIALLICVGGIPAYAEELVPDAGEITGRV
                     LFISSYSYAWETVPEQICGIKEALGDGVQLDYQFMDTKNVETAESEQLFYQTLAYYLK
                     MVPAYDVVIVGDDAAFQFAMTYRDELFAQTPVVFEGVNDGELAKKAAADPLVTGVVET
                     LSYENTITLAKKLYPDARQVVGILDDTVTGEGERKEFQYYDTVFPELDFVEIDASKLS
                     QEELKRQVAALGEESILVYIMCSRDGDGNVYAAAEAIQMLSDVAQIPMFSIVSHGMGK
                     GFLGGEIVSQEQMGKRAGEMAVQILEGTDCAGISIVMEPPKVYCFDENVMRRFGISAS
                     KLPGDAVIINHRETFWEKNRDTIRATLVIAAVALAMVAWLAVDNMRRRKMNEVITRAN
                     EKLSYSAHYDVLTHLKNRSVFMEDIRQRIVSGEEFTVFMYDLDNFKRVNDTYGHNTGD
                     EVLREVALRSLAVEDGHFTPYRLAGDEFVALIDCGDYRVIERYASGLMERLQKPCRMG
                     EADETLGVSLGIAVWPEHGADATELLAAADAAMYTVKKNGKNGYAFYKEPCAP"
     gene            complement(1967..3805)
                     /gene="typA"
                     /locus_tag="ACZO9R_02615"
     CDS             complement(1967..3805)
                     /gene="typA"
                     /locus_tag="ACZO9R_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR0911999.1"
                     /translation="MKMKREDVRNIAIIAHVDHGKTTLVDELLKQSGVFRENQEVQER
                     VMDSNDIERERGITILSKNTAITYKGTKINVIDTPGHADFGGEVERVLKMVNGVVLVV
                     DAFEGVMPQTKFVLMKALERSLPVIVCLNKVDRPEARPNEVVDEVLELFMDLDASEEQ
                     LDCPFLFASARDGYAVRELDDLVNNKKDMTPLFDTILDYIPAPEGDPDANTQVLISTI
                     DYNEYVGRIGIGKVDNGCLKVNQEAVVVNHHEPDKQKRVRISKLYEFDGLNKVDVTEA
                     KIGSIVAISGIADIHIGDTICAPENPVAIPFQKISEPTLSMNFIVNDSPLAGQEGKFV
                     TSRHLRDRLFRELNTDVSLRVEETDSPDSYKVSGRGELHLSVLIENMRREGYEFAVSK
                     AEVLYHTDENGKKLEPMELAYVDVPDEFTGAVIEKLSQRKGEMLNMKPITGGYTRLEF
                     NIPSRGLIGYRGEFMTDTKGNGIINTIFNGYAPYKGEIAYRKLGSLIAFETGESVTYG
                     LFSAQDRGTLFIGPGEKVYAGMVIGSSSRAEDIELNVCKKKHLTNTRSSSADEALTLV
                     PPKILSLEQALDFIDVDELLEVTPESLRIRKRILDPTLRKRAGMKK"
     gene            4062..4691
                     /locus_tag="ACZO9R_02620"
     CDS             4062..4691
                     /locus_tag="ACZO9R_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR0912000.1"
                     /translation="MADKIFENNQVSIMGEIVSDFRFSHEVYGEGFYMVDVAVNRLSN
                     YLDYIPLMISERLIDVNADYIGQIIYVNGQFRSFNRHEEKKNRLVLSVFVRELEFLEE
                     LQEDMKSNQIFLDGYICKEPIYRKTPLGREIADLLIAVNRSYGKSDYIPCICWGRNAR
                     FASGFEVGGHVQVWGRIQSREYVKKISELETEKRVAYEVSVSKVDFLEE"
     regulatory      4756..4930
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
BASE COUNT         1322 a         1036 c         1415 g         1236 t
ORIGIN      
        1 cagaaagagg gaaacaacga gatctgggtg gactgctata atctgacgcg ctttttgtat
       61 aagaaccgtt ccgcggtgca gaaagtgggg gaaagcaggg agtggaagag gacgctgcag
      121 atgacggatc cggcggttgt aaaatatcag aaaaccgtaa aaaaacagtg aaaaaatatt
      181 gacaatagaa gcgggagggt ggaaaatttt gatatacgga tagggaggag gagtgtctgt
      241 gagagaacgt cgatggattc aaaaagggta ctggattttt tttgctattg cacttcttat
      301 ctgtgtggga ggcattccgg catatgcaga agaactggta ccggacgctg gggaaatcac
      361 cggcagggta ttatttatca gttcttattc ctatgcatgg gagacggttc cggagcagat
      421 ttgcggaata aaagaagcgt tgggcgacgg tgtacagctg gattatcagt ttatggatac
      481 caaaaatgtc gagacggcgg agagtgaaca gcttttttac cagacgcttg catactatct
      541 gaagatggtt ccggcgtatg atgttgtgat tgtaggggat gatgcggcgt ttcagtttgc
      601 aatgacgtat cgggatgagc tgtttgcgca gacgccggtt gtttttgagg gagtcaatga
      661 cggggaactg gcgaagaagg cggcagcaga cccgcttgtg acgggcgtgg ttgagacgct
      721 ctcttatgag aatacgatca cgctggcaaa gaagctgtat ccggatgcaa gacaggtggt
      781 aggaatactg gatgatacgg tgaccggaga gggcgaacgc aaggaattcc agtattacga
      841 tactgttttt ccagagttgg actttgtaga gatcgacgct tcaaagctct cccaggaaga
      901 attaaaaaga caggttgctg cgctggggga ggagagcatt ctggtctata ttatgtgcag
      961 cagggatggc gacgggaatg tgtacgcggc ggcggaagcg atccagatgc tgagtgatgt
     1021 ggcgcagatt ccgatgtttt caattgtctc gcacggtatg ggaaaaggat ttttaggcgg
     1081 cgagattgtc tcgcaggaac agatgggaaa aagggcagga gaaatggcgg tgcagatact
     1141 ggagggcacg gattgtgcag ggatctccat tgtgatggag ccgccgaagg tatattgctt
     1201 tgatgaaaat gtgatgcgca ggtttggaat cagtgcatca aagcttcccg gggatgcagt
     1261 catcatcaat cacagggaga ccttctggga aaagaaccgg gatacgatca gggcaacgct
     1321 ggtgattgct gccgtggcgt tggcaatggt ggcatggctg gcggtggaca atatgcgccg
     1381 acgcaaaatg aacgaagtga ttaccagggc aaacgaaaaa ctgtcttaca gtgcacatta
     1441 tgatgtactg acacacctta agaaccgtag tgtctttatg gaagatatca ggcagcggat
     1501 tgtctccggg gaagaattta cggtttttat gtatgatctg gataatttta agcgggtgaa
     1561 cgacacctac ggacacaata caggggacga ggtgctgcgc gaagtggcac ttcggtcgct
     1621 ggcggtggag gatggccatt tcacaccgta ccgcctggca ggagatgagt ttgtagcact
     1681 gatcgactgc ggcgattaca gggtcataga gcgatatgca tctggtttga tggaacgtct
     1741 gcaaaagccg tgcagaatgg gagaggcgga cgagacactg ggagtcagtc tcggaatcgc
     1801 ggtgtggccg gaacacggag cggatgcaac ggaacttctg gcagcggcgg atgctgcgat
     1861 gtatacagtg aagaaaaacg gaaaaaatgg atatgcattt tataaggagc catgtgcacc
     1921 ataggaaacg gtgcacatgg ctccttttta agagtagaaa tacgacttat tttttcatgc
     1981 cggcgcgttt tcttaaggtt ggatcaagaa tacgcttgcg gatacgaaga ctctccggtg
     2041 taacctccag gagctcgtcg acatcgatga aatcgagtgc ctgctccaga cttaaaatct
     2101 taggcggaac cagagtcagt gcctcgtccg cagaagagga acgcgtgttg gtcaggtgct
     2161 ttttcttgca gacattcaat tcgatatcct cggctctgga agatgaacca atgaccatgc
     2221 cggcgtatac cttttcgcca ggtccaataa agagcgtacc gcgatcctgt gcggaaaaca
     2281 gaccgtaggt tacggactca ccggtctcaa atgcgatcag ggaaccgagc ttgcggtaag
     2341 cgatctcgcc cttgtacggt gcatagccgt tgaaaatggt gttgatgata ccgttaccct
     2401 tggtatccgt cataaattca ccacggtaac cgatcaggcc gcgggacggg atgttgaact
     2461 ccagacgtgt gtaaccgccg gtaatcggtt tcatgttcag catttcgccc ttgcgctggc
     2521 ttaacttttc gatgacggca ccggtaaatt catccggtac atcgacataa gcaagctcca
     2581 tcggctctaa cttcttgccg ttctcatccg tgtggtagag aacttcagct ttgctgacgg
     2641 caaactcata accctcgcgg cgcatgttct caatcagtac ggacagatga agctcaccgc
     2701 ggccggatac tttgtaggaa tccgggctgt ccgtttcctc gacacgcaga gaaacatcgg
     2761 tgtttaattc gcggaacaga cggtctctta aatgacggga ggtaacgaat ttaccctcct
     2821 gtccggcgag cgggctgtca tttacgatga aattcatgga cagtgtcggc tcggaaattt
     2881 tctggaacgg aatggcaacc ggattctcag gtgcgcagat ggtatcaccg atatggatat
     2941 ctgcaatacc ggaaatagct acgatggaac cgatctttgc ctctgtgaca tccaccttgt
     3001 tcagaccgtc aaactcgtaa agcttgctga tgcggacgcg tttctgcttg tcaggctcgt
     3061 ggtggttgac aacaaccgcc tcctgattga cttttaagca gccgttgtcg acctttccga
     3121 taccgatacg tccgacatat tcgttgtaat cgatggtgct gatcaatacc tgggtgttgg
     3181 cgtccggatc gccctccggt gcagggatat agtcaaggat cgtgtcgaac agcggagtca
     3241 tatccttttt gttgttcacc agatcgtcaa gctcgcggac tgcgtagccg tctcttgcag
     3301 aagcaaaaag gaacgggcag tcaagctgct cctcggaagc gtccagatcc atgaaaagct
     3361 caagcacttc gtccacaacc tcgttcggac gtgcctccgg acggtcgact ttgttgagac
     3421 acacgataac cggaagagaa cgctcgagcg ctttcatcag aacgaacttg gtctgcggca
     3481 taacgccctc gaaagcgtca acgaccagca caacgccgtt taccatctta aggacacgct
     3541 caacctcgcc gccgaaatca gcgtggccag gggtgtcgat gacgttgatc ttagttcctt
     3601 tataagtaat cgctgtattt ttggaaagaa tcgtgatacc acgctctctc tcaatatcat
     3661 tagagtccat gacacgctcc tgaacctcct ggttctcacg gaacacgccg ctctgtttta
     3721 acaattcatc gaccagagtg gtcttgccgt gatcgacatg ggcgataatt gcaatgttac
     3781 ggacatcttc acgtttcatt ttcatataca aaatctctct ttcctgtgtg aaacctagtc
     3841 taaaaaagta accaatctat tttatcatat tttcggagat gcgcaagtgg tacttgcgac
     3901 aaaatccgcc atttcattcc ctttgttatc gacatgttgc atgaaaattc tggcaccgga
     3961 cgtgataaaa ataatgtgtc aggcagatca taccagcaga acggaacgaa aaagaagaac
     4021 aacgcaaaaa agagtatggt cgtaagaagg gagaaagatt catggcagat aaaatttttg
     4081 aaaacaacca ggtgtcaatt atgggagaga ttgtatccga tttccggttc agtcacgaag
     4141 tgtacggaga gggattttat atggtggatg tggcagtgaa ccgcctgagc aattatctgg
     4201 attacattcc gctaatgata tccgaacggt tgattgacgt aaatgcagat tacataggac
     4261 agatcattta tgtgaacggg cagttccgtt cgtttaaccg gcatgaggag aaaaagaacc
     4321 gccttgtctt atcggtgttc gtgcgggaac tggagttttt ggaggagctg caggaggata
     4381 tgaagagcaa ccagattttt ctggacggat atatctgtaa ggaaccgatt taccgcaaga
     4441 cgccgctggg acgggagatt gcggatcttc tgattgcggt aaaccgttcc tacggaaagt
     4501 cagactatat accgtgtatc tgctggggga gaaatgcgag atttgcttcc ggctttgaag
     4561 tgggaggtca tgtgcaggtg tggggcagaa tccagagcag ggaatatgtc aaaaagatct
     4621 cggagttaga gacagaaaag cgggtggcat atgaggtatc ggtgagcaag gtggattttt
     4681 tggaggaata atcgaaagtt ccgttattga caaccgttgt tgatagtgct atgttaattt
     4741 taactatatg atacatattt tgatagaggt tgcgtgcttt aagagtagtt ttccggatgt
     4801 gacaggcaca ggggaaggaa agcaaaagga aagcatgccg aaagagcaag gcacctgctg
     4861 ccctggttct tgggcataaa gctaagaact ttatgactgt catctttgca gatggggcgc
     4921 tatcggttct gtataatggc atgcagatat gcggcagaag aatcagaccg gctcatcgag
     4981 ccggcttttt tttacttttt tattctgac
//
LOCUS       DBMDQJ010000083         5001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6497_length_5001_cov_1.588367, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000083 DBMDQJ010000000
VERSION     DBMDQJ010000083.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 5001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 5001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5001
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6497_length_5001_cov_1.588367"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            179..1342
                     /locus_tag="ACZO9R_02625"
     CDS             179..1342
                     /locus_tag="ACZO9R_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR0912001.1"
                     /translation="MRYEEKPQITEQEIREAMDFSTAQVKRNLPEFTTKFQKAYSENG
                     FYAPTDNVDWTTGFWTGEIWLSYEDTGKDIYKKAGEIQIDSFLNRIENKIEVDHHDMG
                     FLYSPSCVAGYKLTGNETGRKAAILAADQLIRRFHPVGNFIQAWGAMDAPENYRLIID
                     CLLNLPLLYWASEETGDPKYRDIAEKHIHTAVANVIREDHSTWHTFFFDMKTGAPDHG
                     ATCQGYRDGSAWARGQAWGIYGMALAYKYTEREEYIQKFRDVTDFFLAHLPKDMVPFW
                     DLEFTDGDDQPRDSSSASIAACGMLEMAKYLKPDEAEAYRATAKRLMKALYDGYAVKD
                     PKESNGLVLHSTYSNHSPYNTCNHYGVDECNIWGDYFYMEALMRLKKDWNPYW"
     gene            1369..3393
                     /locus_tag="ACZO9R_02630"
     CDS             1369..3393
                     /locus_tag="ACZO9R_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HIR0912002.1"
                     /translation="MKREAFFDRDASHYYCEDGKAVGAYCREQFPEDVAQTLAVADGV
                     CRKYFLFNSKWDLEQTDEPVQFDGAIDWTYMPADDPEFVWQFNRHRFFMTLGQAYQMT
                     GDEKYARAFMEIAEDWIDHVPLNEKYAAGPWRSLDTGFRGEYWSKAIWLFHDSPTLTE
                     AFLQKYYDSMILQAEHIMECHSAYRYMSNWGVIENHGLFEIGVCLPQSEKTKQFIAFA
                     VKNLEVQVRMQIMPDGVHWEQSPMYHNEVLHCLLDVILLAKRNGIALPDVILRQTEKM
                     AMADVAWLKPDHHIVMMGDSDDVDVRDRISVAAYLFLNPVLRFGGFDRLDYESIWDLG
                     MKAGEEYAVMERRKPDFTSLFLEHSGNTYFRSDWSERANFLHLHSGTMGAGHGHSDKL
                     HIDLVVNGEDVLMDGGRYTYVSGPKRFSYKDPSGHNTITVDDLPFTVCKDSWECSKLS
                     QPVKENFRCMELAEFAQAGQLGYMDLPGGVYVNRKVVYIKPDLYILVDEMYTGDCHTY
                     QQYFHFNNHGAVTLTAPGEKKFPCVAYRGKMAEADFYFVSAQVKAELKRGHIARHYNV
                     EEENTVCKVTKPGDGFTSLITVIDAHPGRPLSIEKLPVRSALKQTDYPETMAEALKIT
                     TGEKEYVVILCHQEVNSPTDLVEADGCMGYGNVIVFDKAEDVLVGDVLNW"
     gene            3420..4130
                     /locus_tag="ACZO9R_02635"
     CDS             3420..4130
                     /locus_tag="ACZO9R_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912003.1"
                     /translation="MRKKLEHWWTYYKGVVIFAVVLAAAVLYVADTMGKKEKTQILMG
                     EVVNNVQDDAVTTQMEEAILAGIGGDPNGEEVVVDTALTMDAAALGQTPIADANTQDS
                     LATITTYVYAHELDFMILEKDVFDYYCNLNAFADLGELLGAGACEALGARIYEKNGVA
                     CGITLTDTAFVKQYGITLLDPVIGIVSGSERKEQAVGMLRWIFEENTGVAAAFSAEEY
                     KAMISQEETGRKDDGKNV"
     gene            4114..4749
                     /locus_tag="ACZO9R_02640"
     CDS             4114..4749
                     /locus_tag="ACZO9R_02640"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR0912004.1"
                     /translation="MGKMFDMDNPVWRFMGKVTDMFFLTCLWLLFSLPVVTIGAATCA
                     LCYCSMTLASNKEGYIREDFVRAFKENFKSATIAWIGMLALGIFFGADLYFYYHLAGG
                     VGTVFFWIFLVLSVIFVFVALYVFPLLAWTDADLKKTLALAFVMSFKNFGWTLLMLTS
                     AALFITAALFVFWPLLFVSAGGIAYVHAKILTFVFSQYEWRDERKDGSDND"
     gene            4730..>5001
                     /locus_tag="ACZO9R_02645"
     CDS             4730..>5001
                     /locus_tag="ACZO9R_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HIR0912005.1"
                     /translation="MAVITIKIQNKDGMTLSVARGEDEAALVYTKEYEEGDEIIFEIS
                     PKNAFYELMADDALGAATVYVTGDVHYRIPFGERKISYSPKTFSGNR"
BASE COUNT         1247 a         1075 c         1558 g         1121 t
ORIGIN      
        1 gcagagatac tttgtagagg gaattgcatc taccggttta aagggctaag accgaaggca
       61 tgtaacaaag ctgtcacaca aaaagagatc gtttgtgtga cggctttgtt ttactctaca
      121 ggaataacgt gttggataaa cataacatga tttgaaaacg gaaaaggaga agaagggtat
      181 gcgatacgaa gaaaaaccgc agatcacaga gcaggagatc agagaggcga tggatttctc
      241 cacggcgcag gtaaagcgca atctgccgga gttcacgacg aagttccaga aggcttacag
      301 cgagaacggg ttttacgcgc cgacggacaa tgtggactgg acgacgggat tctggacggg
      361 cgagatctgg ctgtcctacg aggataccgg gaaagacatc tataaaaaag ccggtgaaat
      421 ccagattgac agctttttga accgcattga aaacaagatc gaagtcgatc atcacgatat
      481 gggatttttg tattccccgt cctgcgtggc gggctataaa ctgaccggga atgagacggg
      541 aagaaaggca gccatactgg cagccgatca gctcatccgc agatttcacc cggtgggcaa
      601 ttttatacag gcgtggggtg cgatggatgc accggaaaac taccggctga tcatcgactg
      661 tcttttaaat ctgccgctgc tctactgggc gtcggaggag accggcgatc cgaagtatcg
      721 ggacattgcg gagaagcata ttcacacggc ggtcgccaat gtgatccgtg aggatcattc
      781 cacctggcac acctttttct tcgatatgaa gacgggggca ccggatcacg gagccacctg
      841 tcagggatac cgcgacggtt cggcgtgggc gagaggccag gcatggggca tctacgggat
      901 ggcgctcgcc tacaagtaca cggaaaggga agaatacatt cagaagttcc gggatgtcac
      961 ggactttttc ctggcgcatc tgcccaagga tatggtgccg ttctgggatc tggagttcac
     1021 ggacggggac gatcagccga gagattcctc ctcggcgtcg atcgcagcat gcggaatgct
     1081 tgagatggca aaatatctaa agccggacga ggcagaagct taccgtgcaa cggcaaaacg
     1141 gttgatgaag gcgttgtacg acggatacgc ggtgaaagat ccaaaagagt cgaacgggct
     1201 ggtgttacac agcacctatt ccaaccactc tccgtataat acctgcaatc attacggggt
     1261 ggacgagtgc aatatctggg gagactattt ttacatggaa gcgctcatgc ggctgaaaaa
     1321 agactggaat ccgtattggt aacaagggaa aaaggaaggt atggcaggat gaaaagagaa
     1381 gcattttttg acagggatgc atcacactat tactgtgagg atggaaaagc ggtcggggcg
     1441 tactgcaggg agcaatttcc agaagatgtc gcgcagacgc ttgcggtggc ggacggtgtg
     1501 tgccggaaat attttctgtt caactccaag tgggatctgg agcagaccga cgagccggta
     1561 cagtttgacg gagcgatcga ctggacgtat atgccggcag acgatccgga atttgtctgg
     1621 cagttcaaca gacaccgctt tttcatgacg ctcggtcagg cgtatcagat gaccggggat
     1681 gagaaatatg cgagagcgtt tatggagatt gcggaggact ggatcgatca tgtcccgctg
     1741 aacgagaagt atgcggcggg accgtggaga agtctagaca ccggctttcg cggggaatac
     1801 tggagcaagg cgatctggct gtttcatgac agcccgacgc tcacggaagc gtttttacag
     1861 aaatattatg acagcatgat cctgcaggcg gaacacatca tggaatgtca ttccgcttac
     1921 cgctatatga gtaactgggg cgtcattgag aatcacgggc tgtttgagat cggcgtgtgt
     1981 ctcccgcaga gcgagaagac aaagcagttt atcgcgtttg ccgtgaagaa tcttgaggtg
     2041 caggtgcgga tgcagatcat gccggacggc gtgcactggg agcagtcgcc gatgtatcac
     2101 aacgaggtgc tgcactgcct gttagacgtg atccttctgg caaagcggaa cggcattgca
     2161 ctgccggacg tgatcctgcg ccagacggag aagatggcaa tggcggatgt cgcgtggtta
     2221 aagccggatc accacatcgt gatgatgggg gacagcgacg acgtggatgt gcgggaccgc
     2281 atcagcgtgg cggcgtatct gtttttaaat ccggtgctgc ggtttggcgg attcgaccgt
     2341 cttgattatg aaagtatctg ggatctcggc atgaaagccg gggaagaata tgcggtgatg
     2401 gagcgcagaa aaccggactt tacatcgctg tttctggaac acagcgggaa cacttatttc
     2461 cggtcggact ggtcagaacg cgccaatttc ctgcatctgc attccgggac gatgggagcg
     2521 gggcacggac attccgataa gctgcacatc gacctggttg taaacggcga ggatgtgctg
     2581 atggacggtg gtagatatac ctacgtttca ggaccaaagc gtttttcgta caaggatccg
     2641 tcaggacaca atacgatcac ggtggatgat ctgccgttta ccgtctgcaa ggattcctgg
     2701 gagtgctcga agctttccca gccggtcaaa gagaatttcc ggtgcatgga gctggcggaa
     2761 tttgcacagg cggggcagct cggctacatg gatcttccgg gcggcgtcta tgtgaaccgg
     2821 aaggtggttt acataaagcc tgacctgtat attctggtgg atgagatgta caccggggac
     2881 tgtcacacct accagcagta ttttcatttt aacaatcatg gtgcggtgac gttgactgcg
     2941 ccgggagaaa agaagttccc atgcgtggcc tatcgtggta aaatggcaga agcggatttt
     3001 tattttgtgt cggcgcaggt gaaagcagag cttaagcgcg gccacattgc aagacattac
     3061 aatgtggaag aggaaaatac ggtgtgtaag gtgacaaagc cgggggacgg ttttaccagc
     3121 cttatcacgg tcatcgatgc acatccggga cgcccgcttt cgattgagaa gcttccggtg
     3181 cgctcggcat taaagcagac agactatccg gagacgatgg cggaggcatt aaagatcacc
     3241 acaggcgaaa aggaatatgt ggtgatcctc tgccatcagg aggtcaattc gccgaccgac
     3301 ctcgtggagg cagacggctg tatgggctac ggaaatgtga ttgtctttga caaggccgag
     3361 gatgtgctgg tgggcgacgt tctgaactgg taggagagaa gcaaaaagga ggatgcgcgg
     3421 tgcgaaagaa actggagcat tggtggactt attataaagg tgtcgtaatt tttgcagtgg
     3481 ttctcgcggc agcggtcctc tatgttgcgg acaccatggg gaaaaaagaa aagacacaga
     3541 ttctcatggg agaagtggtc aacaatgtgc aggacgatgc ggtgacaacc cagatggagg
     3601 aggcgatcct cgccgggatc gggggcgatc ctaacgggga ggaggtcgtc gtggacacag
     3661 cgcttaccat ggatgcagcc gcgctggggc agacgccgat cgccgatgcc aatacgcagg
     3721 attccctcgc aacgatcacg acgtacgtgt atgcgcacga gctcgacttc atgattctgg
     3781 aaaaagatgt gtttgactat tactgtaacc tgaatgcgtt tgctgatctt ggggaactgc
     3841 tgggcgctgg ggcatgtgaa gcgctggggg cgcgcatcta tgaaaaaaac ggtgtggcat
     3901 gcggcatcac actgacagat accgcgtttg tgaagcagta cgggattacg ctcttagacc
     3961 cggtgatcgg gattgtgagc ggatcagaga ggaaggagca ggcggtcggg atgctccggt
     4021 ggatctttga ggagaacacc ggggtggcag ccgcgttttc ggcagaggaa tacaaagcga
     4081 tgatttcgca ggaagagaca gggaggaaag acgatgggaa aaatgtttga tatggacaat
     4141 ccggtctggc gttttatggg aaaggtgacg gatatgtttt ttctgacatg tctgtggctg
     4201 cttttttcac tgccggtcgt aacaatcggg gcggcgacgt gtgcactgtg ctattgctcc
     4261 atgacgttag cgtccaacaa ggagggctac atccgggaag attttgtgcg ggcgtttaag
     4321 gagaatttta aatctgcgac gatcgcctgg atcggaatgc tggcgctcgg aatctttttc
     4381 ggagcggatt tatattttta ttatcacctc gcaggcggcg tcggaacggt gtttttctgg
     4441 atttttctgg tgctgtcggt gatttttgtc ttcgttgccc tgtatgtgtt cccactgctg
     4501 gcatggacag acgcggattt aaagaagacg cttgcgcttg cgtttgtgat gtcgtttaag
     4561 aacttcggct ggacgcttct gatgctgaca tccgcggcac tgtttatcac ggcagcgctg
     4621 tttgtgttct ggccgctgct tttcgtgtcg gccggcggaa tcgcatatgt acacgcgaag
     4681 atcctgacgt ttgttttttc acagtatgaa tggagagatg agaggaaaga tggcagtgat
     4741 aacgattaag atacagaata aggatggcat gaccttaagt gttgccagag gagaggatga
     4801 ggcggcactg gtctacacga aagaatacga ggagggagat gagatcatct ttgagatctc
     4861 cccgaaaaat gccttctacg aactgatggc ggatgatgcg ctcggggcgg caaccgtcta
     4921 tgtgaccggg gacgtccact acaggattcc gttcggggag agaaagattt cctactcgcc
     4981 caagacattt tccgggaacc g
//
LOCUS       DBMDQJ010000084         4986 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6512_length_4986_cov_1.829273, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000084 DBMDQJ010000000
VERSION     DBMDQJ010000084.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6512_length_4986_cov_1.829273"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="ACZO9R_02650"
     CDS             complement(<1..299)
                     /locus_tag="ACZO9R_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HIR0912006.1"
                     /translation="MSFSSEVKTELAKHLGKSRHCQIAELAALIAFEGRIPAAESENR
                     LLMQKYQLLLAELFHIEEIHTEEEARSVFSTVKMYNDATGEAEPEDTVKGLLIQQ"
     gene            complement(354..1232)
                     /gene="rapZ"
                     /locus_tag="ACZO9R_02655"
     CDS             complement(354..1232)
                     /gene="rapZ"
                     /locus_tag="ACZO9R_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HIR0912007.1"
                     /translation="MKFVILTGMSGAGKSTAIKMMEDIGYYCVDNLPIALLEKFVELS
                     SLQENAELQKVAVGIDIRNGQALEEIRDVLARIKKKKVHYEILFLDAEDSVLVKRYKE
                     TRRNHPLSGGDRVDKGIEEERKRLAFLKESADYIIDTSQLLTRELKAELDKIFVQNQD
                     YKNLFITILSFGFKYGIPADSDLVFDVRFLPNPYYVEGLRAKTGNDKEIQDYVFQYEE
                     AHTFLDKLEDMLNFLIPNYIAEGKNQLVISIGCTGGKHRSVTLANALYERLSGQKGYG
                     LKIEHRDIGKDALRGK"
     gene            complement(1284..2192)
                     /gene="murB"
                     /locus_tag="ACZO9R_02660"
     CDS             complement(1284..2192)
                     /gene="murB"
                     /locus_tag="ACZO9R_02660"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR0912008.1"
                     /translation="MKQQLLDTLEQIAGKENIHPNEPMSRHTTFRVGGPADVLVTPEA
                     GKLAAVTGACREAGEPYYIVGNGSNLLVGDGGIRGVVILTRDGMDEISADGVRITAGA
                     GALLSRTASLAASHALTGMEFAAGIPGTIGGAVVMNAGAYGGETRDIIETVTVLDETG
                     AQHVLSVEELELGYRTSCILKKHYIVTQAVLKLAAGEEQAIRARMKELATQRAEKQPL
                     EYPSAGSTFKRPEGYFAGKLIMDAGLRGYRVGGAQVSQKHCGFVVNTGNATAQDVRTL
                     IADVSAEVDRQFQVKLEPEIKMLGEF"
     gene            complement(2189..3586)
                     /locus_tag="ACZO9R_02665"
     CDS             complement(2189..3586)
                     /locus_tag="ACZO9R_02665"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR0912009.1"
                     /translation="MERMNAWEKYPEGKKRNEVFDFAEEYRKFLSDCKTERECVTYFA
                     QKAEKAGFVNLDTVLEKGTKLAAGDKVYANNMGKGLALFVIGEKSMEHGMNILGAHID
                     SPRMDLKQDPLYEDTDFAMLDTHYYGGIKKYQWVTLPLALHGVIAKKDGTVVKVSVGD
                     KPGDPVFGVSDLLIHLSGEQMEKKAAKVIEGENLDLIIGSIPAEADEKDDVKEKVKAN
                     IMKILAKEYDIEEEDFLSAEIEVVPAGEARDYGFDRSMIMGYGHDDRVCAYPSFEAIA
                     AMEKPEITSVCLLVDKEEIGSVGASGMQSRFFENTVAEVMNAAGTYSELALRRALKNS
                     SVLSSDVSAAFDPNFPSVMTKRNAAYFGRGLVFNKYTGARGKSGSNDANAEYVGALRA
                     IMDRNEVSFQTAELGKVDQGGGGTIAYILANYGMRVIDSGVAVLNMHAPWEIISKVDL
                     YEAYRGYIAFLKEQA"
     gene            complement(3616..4560)
                     /gene="hprK"
                     /locus_tag="ACZO9R_02670"
     CDS             complement(3616..4560)
                     /gene="hprK"
                     /locus_tag="ACZO9R_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR0912010.1"
                     /translation="MSGVGVAKVAQILDLCNFIPQMELKGHRIMIRDVNRPALQLSGY
                     FEHFEQSRVQIIGTVEYTYLQQLDEKKKEAIYREFMAYDIPCVIFCRDLKPDEMFLKI
                     AEESNLPVFGTKRSTSEFMAELIYCLSEQLAPCITIHGVLVDVYGEGLLIMGESGIGK
                     SEAALELVRRGHRLVTDDVVEIRKINEHTLIGTSPDITRYFIELRGIGIIDVKTLFGV
                     EGVKEKQQIDLVIKLEDWKKDNEYDRLGLEEEYTEFLGNKVVCHSLPIRAGRNLAVIC
                     EAAAVNHRQKKMGYNAAQELYRRVQENLTKKSDDEEDE"
     gene            complement(4628..>4986)
                     /locus_tag="ACZO9R_02675"
     CDS             complement(4628..>4986)
                     /locus_tag="ACZO9R_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR0912011.1"
                     /translation="KDDNHRTRGLYYHNVEIPIDRGSEGFKLITRIQDEAHRFAIEYH
                     RSLRSKEQVHSVLDDIPDIGPARRKALMKKYQSLEAIREATEEDLAQTDSMSPQAARS
                     VYRFFREKERENQPSD"
BASE COUNT         1016 a         1547 c         1058 g         1365 t
ORIGIN      
        1 ctctgctgaa tgaggagtcc cttgactgta tcttccggct ccgcctcccc cgtggcatcg
       61 ttgtacattt taaccgtgga aaaaacgctg cgcgcctcct cctcggtatg gatttcttct
      121 atatgaaaca gttctgctaa cagcagctga tatttttgca tcagcagccg gttctccgat
      181 tccgccgcag ggattcttcc ctcaaatgca atcagcgccg ccagctctgc aatctggcaa
      241 tgtctgcttt ttcccagatg ttttgccagt tccgttttta cctcacttga aaatgacata
      301 gcttactcca accgtccctc gcgtccatcg gctcttcctt ttgtctgcgg ggtctatttc
      361 ccgcggagcg catctttacc gatgtcgcgg tgctctattt tcagtccgta tcccttttgt
      421 ccggaaagcc gttcatacag cgcattggcg agcgtgaccg accggtgctt tcctccggtg
      481 cagccaatgg agattacaag ctggttcttc ccttctgcaa tataattcgg aatcaggaag
      541 ttcagcatat cctcgagctt gtccaaaaac gtatgcgcct cctcatactg gaacacatag
      601 tcctgaatct ccttgtcatt gcccgtcttt gcccgcaatc cttccacata atacggattc
      661 ggcagaaaac gcacgtcaaa gaccagatcg gagtccgccg gaatcccata cttaaatcca
      721 aacgacagaa tcgtgataaa cagattctta tagtcctgat tctgcacaaa aatcttatcc
      781 agctccgctt tcagctccct ggtcagaagc tggctggtat ctataatata gtctgcactc
      841 tctttcaaaa aagccagacg ctttctctct tcttcaatgc ccttgtccac ccggtctccg
      901 ccggacagcg gatggtttct tctggtctcc ttgtagcgct tgaccaatac agaatcctct
      961 gcatccagaa acagaatctc gtagtgaacc ttcttttttt tgatccgcgc aagcacgtcg
     1021 cgtatctctt ccagtgcctg gccgttccgg atgtcaatac cgaccgccac tttctgcagt
     1081 tctgcatttt cctgcaggct cgacagctcc acgaactttt caagcaatgc aatcggaaga
     1141 ttatccacac agtagtatcc gatatcttcc atcatcttaa ttgccgtact ctttccggct
     1201 ccggacattc cggtcaaaat cacgaacttc atgccagtca ctctccattc ctgcgtatcc
     1261 tgctgttatc tttgtctatc gggctaaaat tcgccaagca tcttaatctc cggttccagc
     1321 ttcacctgaa actgtctgtc cacctctgcg ctcacatccg caatcagcgt cctcacatcc
     1381 tgtgcggtcg cattccctgt gttgaccaca aatccgcagt gtttctgcga tacctgtgct
     1441 ccgccgaccc ggtatccgcg caatcccgca tccatgatca gctttccggc aaaatatccc
     1501 tccggccgtt taaatgtgct tcccgcactc ggatattcga gcggctgctt ttcggcgcgc
     1561 tgcgtggcaa gttccttcat ccgtgcccgg atggcctgct cctctcccgc tgcaagcttc
     1621 aataccgcct gtgtgacaat atagtgcttc tttaaaatgc agctcgtgcg gtagccaagc
     1681 tccagctcct ccacggacag cacatgctgt gctcccgtct catcgagcac agtcacagtc
     1741 tctatgatgt cccgcgtctc tccgccgtaa gctccagcat tcatcacaac tgcaccgccg
     1801 atcgttcccg gaatcccggc tgcaaattcc atcccggtca gcgcatgtga cgcagcaagc
     1861 gacgccgtgc gtgacaggag tgctcccgct cccgccgtaa tccgcacgcc gtctgccgaa
     1921 atctcatcca tcccgtctct ggtaagaatg accaccccac ggatgccgcc gtctcccacg
     1981 aggaggttac tgccgtttcc gacaatgtaa tacggctctc ccgcctcccg gcatgcaccg
     2041 gtaactgctg caagcttccc cgcttccggc gtcaccagca catccgccgg accgcccaca
     2101 cgaaaggtcg tgtggcggct catcggctcg ttcggatgaa tattttcttt tcctgcgatc
     2161 tgttctaacg tatccaataa ctgttgtttc atgcctgttc ctttaaaaat gcaatatagc
     2221 cgcgatatgc ctcatacaga tccaccttgc tgatgatctc ccacggtgca tgcatgttca
     2281 gcactgccac accgctgtca atcacacgca tgccatagtt ggcaagaatg taggcgatcg
     2341 ttccgccgcc gccctggtct accttgccga gttctgccgt ctggaatgac acctcgttgc
     2401 ggtccatgat cgcgcggagt gctcccacgt actctgcatt cgcatcgttc gatccggatt
     2461 tgcctctggc tccggtatat ttgttaaaca caagtcctct tccaaaatat gctgcgttgc
     2521 gcttcgtcat cacggacggg aagttcgggt caaatgccgc cgatacatcc gaagagagaa
     2581 cgctcgagtt cttcagtgca cgtctgagtg ccagttcaga atacgttcct gccgcattca
     2641 tcacctctgc gaccgtattc tcgaagaaac gggactgcat accgctcgca ccgacacttc
     2701 cgatctcttc cttgtctacc agaaggcata cacttgtgat ctccggcttc tccatcgccg
     2761 caatcgcctc aaaggaagga tatgcgcaca ctctgtcatc gtgtccgtag cccataatca
     2821 tgcttctgtc aaagccgtag tctctcgctt cgccggccgg tacgacctca atctccgcag
     2881 agaggaaatc ctcttcctct atatcatatt cctttgcaag gatcttcatg atattggctt
     2941 ttaccttttc cttgacgtcg tctttttcat ccgcttccgc cggaatgctg ccgatgatca
     3001 gatcgaggtt ttctccctca atgaccttgg ctgccttttt ctccatctgc tctccggaaa
     3061 ggtggatcaa aagatcgctc acgccgaata ccggatcacc cggcttgtct ccgacactca
     3121 ctttgaccac ggtgccgtcc tttttcgcaa tgacgccgtg cagagccagc ggcagtgtca
     3181 cccactggta cttcttgata ccgccgtaat agtgggtatc cagcatcgcg aaatccgtgt
     3241 cttcatacag cgggtcctgc tttaagtcca tgcgcgggga atcaatatgt gcgccgagaa
     3301 tattcatgcc gtgctccatg ctcttctcac cgattacgaa cagggcaagt ccttttccca
     3361 tattgtttgc atatacttta tctccggctg caagctttgt acccttctcc agcacggtat
     3421 ccagatttac gaatcccgcc ttctctgctt tctgtgcaaa ataggtgacg cactcgcgct
     3481 ccgtcttgca gtctgacaaa aacttacggt actcctctgc aaaatcaaaa acttcgtttc
     3541 tcttttttcc ttccgggtat ttctcccatg cgttcattct ttccattgct gttgtttctc
     3601 ctaacctata ttgatctatt cgtcttcctc atcgtcggac ttcttcgtca ggttctcctg
     3661 tacacggcgg tacagctcct gtgcggcatt gtaacccatc ttcttctgtc tgtggttgac
     3721 tgccgccgcc tcacagatga ctgcaaggtt acgtccggcg cggatcggaa gcgaatgaca
     3781 tacgacttta ttgccgagga actctgtgta ctcctcctca agtcccagac ggtcatactc
     3841 gttatccttc ttccagtctt ccagcttgat gaccagatcg atctgctgct tctccttgac
     3901 tccctcgaca ccgaacagcg tcttcacgtc aatgatgccg attccgcgca gctcgataaa
     3961 gtaacgtgtg atgtccggtg aagtaccgat cagcgtatgc tcgttgatct tgcggatctc
     4021 cacgacatca tcggttacca ggcgatgtcc tctgcgcaca agctcgagtg ccgcctcgct
     4081 cttaccgatg ccgctctctc ccataatcag aagtccctcg ccgtagacat ctacaagcac
     4141 gccgtgaatg gtgatgcacg gtgccagctg ctcgctcagg cagtagatca gctccgccat
     4201 gaactcggat gtgcttcgct tggttccgaa taccggcaga ttgctctcct ccgcaatttt
     4261 gaggaacatc tcgtccggct ttaaatcacg gcagaaaatg acgcacggaa tatcgtaagc
     4321 cataaactcc cggtagatcg cttctttttt cttctcatcc agctgttgca gatacgtgta
     4381 ttccacggta ccgatgatct gcacacggga ctgctcaaaa tgctcgaaat agccgctcag
     4441 ctgcaatgcc ggacggttga catcacggat catgatccga tgtcctttta attccatctg
     4501 cggaataaaa ttgcacagat cgagaatctg cgccactttt gctacaccaa caccactcat
     4561 cccttacgct cctttgtatg tgatttactt ttgtgcaaat tataccatag gcacatggaa
     4621 tcttcaacta atcggacggc tggttttccc gttctttctc ccggaaaaac cggtaaaccg
     4681 agcgcgccgc ctgcgggctc atgctgtccg tctgtgcaag gtcttcctcc gtcgcctccc
     4741 ggattgcctc cagcgactga tattttttca tcagcgcctt gcgccttgcc ggtccgatat
     4801 ccggaatgtc atccagcacg gaatgtacct gctccttgct ccggagcgac cggtggtact
     4861 caatcgcaaa acggtgcgcc tcgtcctgaa ttcgcgtaat cagcttaaat ccctcgcttc
     4921 cacggtcgat gggtatctct acattatgat agtatagccc gcgcgtgcgg tgattatcat
     4981 ctttga
//
LOCUS       DBMDQJ010000085         4977 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6526_length_4977_cov_1.222108, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000085 DBMDQJ010000000
VERSION     DBMDQJ010000085.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4977)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6526_length_4977_cov_1.222108"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /gene="infB"
                     /locus_tag="ACZO9R_02680"
     CDS             <1..1415
                     /gene="infB"
                     /locus_tag="ACZO9R_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR0912012.1"
                     /translation="KEAGGITQHIGASVVSINGQNITFLDTPGHEAFTAMRMRGANST
                     DIAILVVAADDGVMPQTIEAINHAKAAGVEIIVAINKIDKPSANIERVKQELSEYELI
                     PEDWGGSTVFCPVSAHTKEGIENLLEMILLTAEVLELKANPKRNARGLVIEARLDKGR
                     GAVATVLVQKGTLKVGQPIACGSCYGKVRAMIDDQGRRVKEAGPSTPVEILGLSSVPE
                     AGETFVAMDTEKEARAFAETYISEGKNRLIEDTKAKMSLDDLFSQIQSGNVKELDIIV
                     KADVQGSVEAVKQSLEKLSNEEVVVKVIHGGVGAINESDVSLASASNAIIIGFNVRPD
                     PMAKTTADKENVDMRLYKVIYNAIEDVEAAMKGMLDPIFEEKVLGHAEVRQIFKASGI
                     GNIAGSYVLDGVFQRGCKVRISRAGEQIFEGNLASLKRFKDDVKEVKAGYECGLVFEG
                     FNDIAELDIVEAYTMVEVPR"
     gene            1449..1844
                     /gene="rbfA"
                     /locus_tag="ACZO9R_02685"
     CDS             1449..1844
                     /gene="rbfA"
                     /locus_tag="ACZO9R_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR0912013.1"
                     /translation="MRKNSIKNTRINGEVLRELSNIIRGEIKDPRINPMTSVVTVEVA
                     PDLKTCKAYISVLGDEESQKATLAGLRSAEGYIRRQLAHSINLRNTPEITFVLDQSIE
                     YGVRMSKMIDDVTKDIPDRADEAEEETEE"
     gene            1841..2791
                     /locus_tag="ACZO9R_02690"
     CDS             1841..2791
                     /locus_tag="ACZO9R_02690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR0912014.1"
                     /translation="MTNFDDFLAEAHTVAIGGHIRPDGDCVGSCLATYNYIKTWYPDI
                     DVTVYLEPIPNIFKFMARSNEIISDFSGDTVYDLFIAQDCGDTGRLGGAAHFFEQAKH
                     TVCVDHHISNQSFAQDNYIFPQASSASELVYELLPKERITKEIAECIYTGMVHDTGVF
                     QYSCTSRKTMEIAGALMECGINYPKIVDDTFYTKTYNQNRIMGLALLKSRLHLNGKCI
                     SSVITEEEMKQYDVLPKHLDGIVNQLRVTKDVEVAVFLYETGDGTFKVSTRSREVMDV
                     SKVAVKYGGGGHIRAAGFSMTGDADAIIEQIVSDLAEQIK"
     gene            2804..3727
                     /gene="truB"
                     /locus_tag="ACZO9R_02695"
     CDS             2804..3727
                     /gene="truB"
                     /locus_tag="ACZO9R_02695"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR0912015.1"
                     /translation="MMNGIINIYKEKGFTSFDVVAKLRGILKTRKIGHTGTLDPDAEG
                     VLPVCIGKATKICELLTDKTKEYEAVMLLGRTTDTQDVTGETLTQQEVRCSAKEVEKA
                     VLSFAGDYMQVPPMYSALKVDGRKLCDLARAGVTVERAARPVHIFSIEILSIELPRVR
                     MRVCCSKGTYIRTLCQDVGEVLGCGACMESLLRTRVGEFALADAIKLSEVEAYVIAHR
                     SDAAETEEMQLPFLQAVDSVFVKYESATVDGQFRKYLYNGNRLHPEMFLEEHESMRTA
                     PVRVYDDEGVFFGIYEYQAEKEDYKPLKVFK"
     gene            3731..4657
                     /locus_tag="ACZO9R_02700"
     CDS             3731..4657
                     /locus_tag="ACZO9R_02700"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR0912016.1"
                     /translation="MKYIRNTTDFYIEENTVLSLGKFDGIHRGHEQLLQYFAKKKDEG
                     LAGAIFTFDIPPRRNVQNVEAKVLTTNEEKRHMFEQLGIDYVVECPFTQEIMCMEPED
                     FIAKIVRQLHVKCMVVGEDFHFGHNRRGDYHMLRQYAGKYGYEVIVVEKIKEDSRDIS
                     STFVREEIAAGNIEKANHLLGYRYFVTSRVLHGNQIGRTIGVPTINQLPPKEKLLPPN
                     GVYVTEAYIGDRWYRGISNVGCKPTIEGEYPVGVETHLLDFAEDVYDKMVTVEFLSRV
                     RSERKFASIEALKEQMQNDVAYGRAYFEKHTS"
     gene            4785..>4977
                     /locus_tag="ACZO9R_02705"
     CDS             4785..>4977
                     /locus_tag="ACZO9R_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912017.1"
                     /translation="MKARLARIAGLVLAGTLVVSTAAIDQKSKNTDADISATVASGVT
                     SALSGSLSDATMGVAGVSGM"
BASE COUNT         1328 a         1043 c         1510 g         1096 t
ORIGIN      
        1 acaaagaggc aggcggtatc acacagcata tcggtgcatc tgttgtttcg atcaacggtc
       61 agaacatcac attcctggat acaccgggac atgaggcatt cacggcaatg cgtatgcgtg
      121 gagcaaattc caccgatatt gcgattctgg tagtcgctgc agatgacggt gtcatgccgc
      181 agacgatcga agcaatcaac catgcaaagg cggcgggagt tgagattatc gttgccatca
      241 ataagattga taagccgagt gccaatattg agcgcgtaaa gcaggagctg tccgagtacg
      301 aactgattcc ggaagattgg ggcggaagca cggtattttg tccggtatct gcacatacta
      361 aggaaggtat cgagaacctt ttggagatga tccttctgac cgcagaggta cttgagttaa
      421 aggcaaatcc gaagcgtaat gcgagaggcc ttgtcattga ggctcgtctg gataagggac
      481 gcggtgcagt tgccaccgtt ctggtacaga agggtacctt aaaggttggt cagccgattg
      541 catgcggaag ctgctacggc aaagtgcgtg caatgattga cgaccaggga agaagagtca
      601 aggaagcggg accgtcaaca ccggtggaga ttctcggact ttcatcggta ccggaggcag
      661 gcgagacatt cgttgccatg gatacggaga aggaggcaag agcatttgcc gagacctata
      721 tttccgaagg caagaaccgt ctgatcgaag ataccaaggc gaagatgtcg ctggatgatc
      781 tgttctcgca gattcagtcc ggtaacgtca aggaactgga tattattgtt aaggcagatg
      841 tacagggttc tgtggaggct gtaaagcaga gtctggagaa actgtccaac gaagaggtag
      901 tagtaaaagt aattcacggc ggtgtcggtg ccatcaacga gtccgatgtc agccttgcat
      961 ccgcatccaa tgcgatcatc atcggattca acgttcgtcc ggacccgatg gcgaagacaa
     1021 cagcggacaa ggagaacgtc gatatgcggc tctacaaggt catctacaat gctatcgagg
     1081 atgtagaggc ggcaatgaag ggaatgctcg acccgatctt cgaggagaag gtgctcggtc
     1141 atgcggaggt gcgtcagatc ttcaaggcat ccggaatcgg aaatatcgca ggttcctatg
     1201 tgcttgacgg tgtgttccag agaggctgca aggttcgtat ttcccgcgcc ggagagcaga
     1261 tcttcgaggg caatctggca tccttaaagc gtttcaagga cgatgtgaag gaagtaaaag
     1321 ccggatacga gtgcggtctt gtctttgaag gctttaatga cattgcagag cttgatatcg
     1381 ttgaggcata cacgatggtg gaggtaccgc gttaacagac gttttgcgtg tatggaagga
     1441 gctagaaatt gagaaaaaac agtattaaaa atacccggat caacggggaa gtgctgcgcg
     1501 agttgtcgaa tatcatccgc ggggagatca aggacccgcg gatcaatccg atgaccagcg
     1561 tggtcaccgt ggaggttgca ccggatctga aaacgtgcaa ggcttatatc agcgtgctcg
     1621 gggatgagga atcccagaag gcaacgctcg caggtcttag aagtgcggag ggttacatcc
     1681 gcaggcagct tgcccacagc atcaacctta ggaatacgcc ggagatcaca tttgtgctgg
     1741 atcagtccat cgagtacggt gtgcgcatgt ccaagatgat tgacgatgtg acaaaggata
     1801 ttcctgatcg ggcggatgag gcggaagagg aaacagaaga atgacgaatt ttgatgattt
     1861 tctggcagag gctcatacgg ttgccatcgg cggacacatc cgtcccgatg gtgactgtgt
     1921 gggctcctgc cttgctactt ataattacat taaaacatgg tatcccgaca ttgatgtgac
     1981 ggtttatctg gagccgattc ccaatatttt caaatttatg gcgcgctcca atgagatcat
     2041 ctccgatttt tccggggata cggtatacga tctgtttatc gcgcaggact gcggggatac
     2101 gggacgtctg ggcggtgcag cgcatttctt tgagcaggcg aagcataccg tctgtgtgga
     2161 tcaccacatc agcaatcaga gctttgcaca ggacaactac attttcccgc aggcaagttc
     2221 cgcgtcggag cttgtctatg aactgcttcc aaaggagcgc atcacaaagg agattgccga
     2281 gtgcatttat accggaatgg tacatgatac cggcgtcttc cagtattcgt gtacgtcgag
     2341 aaagacgatg gagatcgccg gagcgctgat ggagtgcggg atcaattatc cgaagattgt
     2401 ggatgatacc ttctacacaa agacatacaa ccagaaccgc attatggggc ttgccctgct
     2461 aaagagcagg ctgcacctga acggaaaatg tatttccagc gtgatcacgg aggaggagat
     2521 gaagcagtac gatgtgctgc caaagcatct ggacggcatt gtgaatcagc tccgcgtgac
     2581 caaggatgtg gaagtggcag tctttttgta tgagacggga gacggcacgt ttaaggtgag
     2641 caccagatcc agggaagtga tggatgtctc gaaggtggcg gtaaagtacg gcggtggcgg
     2701 acacatccgg gcagccggat tttccatgac tggcgatgca gatgccatca tcgaacagat
     2761 tgtttcggat ctggcagaac agataaagtg agaaaaaagc agtatgatga atggaattat
     2821 caatatctat aaggaaaagg gatttacctc gtttgacgtc gtggctaagc ttcggggaat
     2881 cttaaagacc agaaagatcg gacacacggg aacgctggat ccggatgcgg agggggtgct
     2941 cccggtgtgt atcggcaagg cgacgaagat ctgcgaactg ctgacggaca agacgaagga
     3001 atacgaggct gtcatgctgc ttggaagaac gacggacacg caggatgtga cgggcgagac
     3061 cctgacgcag caggaggtgc gctgcagtgc aaaggaagtg gaaaaagcgg ttctctcgtt
     3121 tgcaggtgat tatatgcagg tgccgccgat gtattctgcc ttaaaggttg acggcaggaa
     3181 gctgtgtgat ctggcacggg ccggcgtgac cgtggaacgt gcggcgcgtc cggttcatat
     3241 tttttccatc gagatccttt cgatcgaact gccgcgtgtc aggatgcgtg tctgctgttc
     3301 caagggaacg tatatccgca cgctctgcca ggatgtgggg gaggtgctcg gctgcggtgc
     3361 gtgcatggaa tcgctgctcc gcacgagagt cggagagttc gcgcttgcgg acgccataaa
     3421 gctgtccgag gtggaggcgt acgtgattgc acacaggtcg gatgcggcgg agacagaaga
     3481 gatgcagctt ccgttccttc aggcggtgga ttccgtcttt gtaaaatacg agagcgcgac
     3541 ggtggacgga cagttccgca agtatttata taacggaaac cggctgcacc cggagatgtt
     3601 tttggaggaa catgagagca tgagaacggc gccggtccgc gtctatgacg atgagggcgt
     3661 atttttcggt atttatgaat atcaggcgga aaaggaagac tataaaccat taaaggtgtt
     3721 taagtagatc atgaaatata tcaggaatac aacggatttt tatatagaag agaatacggt
     3781 gctttctctc ggcaagtttg atgggattca cagaggacat gagcagctgc tgcagtattt
     3841 tgcaaagaaa aaggacgaag gtctcgcggg agcgattttt accttcgaca ttccacctcg
     3901 cagaaatgtg cagaatgtgg aggcgaaggt gctgaccaca aacgaagaaa aaaggcacat
     3961 gtttgagcaa ctggggattg attatgtggt ggagtgcccg tttacgcagg agatcatgtg
     4021 catggagccg gaggatttca ttgcaaagat tgtgcggcag cttcatgtaa agtgcatggt
     4081 tgtgggggaa gactttcatt tcggacataa tcgcaggggc gattaccata tgctgcggca
     4141 gtatgccgga aaatacggat acgaagtgat cgttgtggag aaaataaaag aagacagccg
     4201 ggacatcagc agcacgttcg tgagggaaga gatcgcagcc gggaatatcg agaaggcaaa
     4261 tcatcttctg ggataccgtt actttgtgac cagcagagtc ctgcacggca accagatcgg
     4321 aagaacgatc ggggttccga ccatcaacca gctgccgccg aaggagaagc tgctgccgcc
     4381 gaacggtgtc tacgtgacgg aggcatacat cggagaccgg tggtaccgcg gcatcagcaa
     4441 cgtggggtgc aagcctacga ttgagggaga gtatcctgtc ggggtggaga cacatctgct
     4501 ggatttcgcg gaagatgtct acgataagat ggtgacggtg gagttcttaa gcagagtccg
     4561 cagcgagcgg aaatttgcat cgattgaggc gctcaaagag cagatgcaga acgatgttgc
     4621 ctatggccgt gcctattttg aaaaacacac ctcgtaataa ttattacata aatgttacaa
     4681 aaatatgttg acatcgtaaa aaatcgttgc tatactggac gttagattta aaaatgagca
     4741 caaactagaa ttgcgcagaa gacggcgcaa gaatggagag tcatatgaaa gcacgattag
     4801 cgagaatcgc aggtttggtc ctggcgggaa ctctggtggt ttccacagcg gcaatagatc
     4861 agaagtcaaa gaatacagat gcggatatca gtgcaaccgt ggcgtccggt gttaccagcg
     4921 cactgtccgg aagcctcagt gacgcgacga tgggtgtggc cggtgtttcc ggaatgg
//
LOCUS       DBMDQJ010000086         4951 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6562_length_4951_cov_1.570515, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000086 DBMDQJ010000000
VERSION     DBMDQJ010000086.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4951)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6562_length_4951_cov_1.570515"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(152..526)
                     /locus_tag="ACZO9R_02710"
     CDS             complement(152..526)
                     /locus_tag="ACZO9R_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912018.1"
                     /translation="MDSVKRMGQTGGQEFCPDRKQTGTMVASPNEERPEVEAAGRELS
                     EAHVIETLRQKSAYRYRNVQQLRLLVWLLECGLAGLLGALFLLAGRERGQQWELIPSG
                     RRIVAYIRRADGKKNGLVSSIK"
     gene            complement(606..854)
                     /locus_tag="ACZO9R_02715"
     CDS             complement(606..854)
                     /locus_tag="ACZO9R_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912019.1"
                     /translation="MRREEEVRLTHSGIFAKDGRRCVSVRFERGRDVAEAILPACRVT
                     KNEGFTDEEVRGLEQYLESKNDEIFAKAKEINPIRGIL"
     gene            complement(879..3539)
                     /locus_tag="ACZO9R_02720"
     CDS             complement(879..3539)
                     /locus_tag="ACZO9R_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR0912020.1"
                     /translation="MSDKANIAAAVLAHVDAGKTTLSEALLYESGMIRRAGRVDNKDA
                     FLDTFALEKERGITIFSKQAVLKLPEETVTLLDTPGHVDFSAEMERTLQVLDYAILVI
                     SGADGVQAHTRTLFGLLAKYEVPVFLFVNKMDQPGTDQEALLLELKKELHENCVDFSD
                     QESAAFAENVAVCDEQVLEQYLAGEAVGTEQIKRLIRERKLFPCYFGSALKMQGVKAF
                     LVDFIRYASIPAYGETFGARVFKIARDAQGNRLTYLKVTGGSLKVRQVIGEEKINQIR
                     IYSGEKYETAEEVGAGGVCAVTGLLRTFPGEGLGAEKASRLPVLEPVLTYQIILPEEV
                     SPAVMLPRLRELEEEEPQLHIVWDETLKEIQVQVMGEVQIEVLSELIRQRYGVQVTFG
                     AGRIVYKETIAGTVEGVGHFEPLRHYAEVHLLLEPGEPGSGLVFETDCSEDVLDRNWQ
                     RLVLTHLREKEHVGVLTGSALADMKITLVAGRAHTKHTEGGDFRQATYRALRQGLMQA
                     ETVLLEPWYDFRLSLPAGAVGRAMNDIDRMKGTIAYSADGCIVGSAPVSAMQNYQREV
                     TAYTAGEGKLSVALKGYFPCHNTEEVVAQIGYDAQSDLENTADSVFCAHGAGFIVPWD
                     QVFSYMHLPPVPGQDKSGEEWYPGQWLPDAANFHAKTGEEEAWIGTEEIDRILDRTYN
                     ANKKDKDAGATNGYKKKRIIESSPASASGGGKQKDAGVEYLLVDGYNVIFAWQELAEL
                     ARVNMDSARGRLLDILCNYQGIRRCELIVVFDAYRVEGHQTEACDYHNIHVVYTKEAE
                     TADAYIERFAHEHASRYRVTVATSDGLEQIIIRGAGCILLSAREFEQEVKEKNRQLQA
                     EYDSRKDHGKVYLRDQMPSQVLDGSLEEQQ"
     gene            complement(3558..>4951)
                     /locus_tag="ACZO9R_02725"
     CDS             complement(3558..>4951)
                     /locus_tag="ACZO9R_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR0912021.1"
                     /translation="LAVSRPGQEVLERTACQMDEAKGDLVLRFEVGFPANGRTVNARE
                     LEKIFFTFLPECVSHSLFYKALDEKAVYRAADLAEDQQYIRDHLKEQGLVAFVADASI
                     LPRESGVSGRPMKDAVKFTAPESMKVSFALPHAGKIQGMGIKKGITLIVGGGYHGKST
                     LLKALELGVYNHIAGDGREYVITDDTAMKIRAEDGRSIKKVDISMFINDLPNGKDTSA
                     FRTEDASGSTSQAANVVEAMEAGAETFLIDEDTSATNFMIRDELMQRVVNREDEPITP
                     FIDRIRELYEQYGISTILVAGSSGSYFHVADCIVQMNRYKPQEITAFAKEEAGRFPLP
                     EVKPPKQAAPGFERAVRPDRAFKEDARMKLKTMGRDGISINRDTIDVRYVEQLADTEQ
                     LAALAYFLKYAEIHVFNGKRTLRESVSLLMQYVEEHGLAAVTESSYVPCGLAMPRAQE
                     IFACINRYRRISL"
BASE COUNT         1018 a         1646 c         1076 g         1211 t
ORIGIN      
        1 acttgagctt gcggtatact ttccatccaa agattaccgg catagccagc accagatatg
       61 cagtcgataa cattccggct gctccaccaa cgatcatcag aatccatatt aaaatattca
      121 tagtttttat cctctcataa aataataatt gctattttat ggaggataca aggccgttct
      181 tttttccgtc ggccctcctt atgtatgcta caatccttct tcccgaggga atcagttccc
      241 actgctgccc gcgctctctc cccgccaaaa gaaacagcgc gcccaaaagt cccgccagac
      301 cacattccag aagccacact aggagccgaa gctgctggac attccggtag cggtaggcac
      361 tcttctgcct cagcgtctct atgacatgcg cctcactcag ttccctgcct gcggcttcca
      421 cttccgggcg ctcctcgttc ggtgacgcga ccatggtgcc ggtctgctta cggtccggac
      481 aaaattcctg tccaccggtc tgccccatcc gcttcacact gtccaaaggt ccggactccc
      541 gcactccgtt tggcagtgcc gccgccacaa gcatcgcggc tatgagcaaa aggcagagat
      601 ggcatttaaa gaattcccct gatcggattg atttcctttg cctttgcaaa aatctcatca
      661 tttttactct ccagatactg ctccagtccg cggacttctt cgtccgtaaa gccctcattc
      721 ttcgtcacgc ggcatgccgg caatatggct tccgcgacat cccttccccg ctcaaaacgc
      781 acagacacac agcgtctgcc gtcttttgca aagatacccg aatgtgtcag tctgacttct
      841 tcttcccttc tcattccaat ctccattccg gaacaaaatt actgctgttc ctccaaagat
      901 ccgtcaagca cctggcttgg catctgatcc cgcagataga cctttccatg gtcttttctg
      961 ctgtcgtact ctgcctgcag ctgccggttc ttctccttta cttcctgctc aaactctctc
     1021 gccgagagca ggatgcatcc ggctccgcgg ataataatct gctccagccc gtcggaagtg
     1081 gcaaccgtca cacggtatct cgacgcgtgt tcatgggcga aacgctcaat atacgcgtcc
     1141 gccgtctccg cctccttcgt gtacaccaca tgaatattgt gataatcaca cgcttccgtc
     1201 tgatgtccct cgacacggta cgcatcgaag accacaatca gctcacatct gcggattccc
     1261 tgatagttgc agagaatatc caaaagcctc ccgcgcgcgc tatccatatt cactctggca
     1321 agttccgcaa gctcctgcca cgcaaaaatc acattatagc cgtccaccag cagatattcc
     1381 acgccggcat ccttctgctt cccgcctccg gaagcagaag cgggactgct ttctatgatg
     1441 cgcttctttt tatagccgtt cgttgcgccg gcatctttgt ctttcttatt agcattgtat
     1501 gtccggtcaa ggattctgtc aatctcttcc gtgccgatcc aggcttcttc ctcccccgtc
     1561 tttgcgtgga aattcgccgc atccggcagc cactgtccgg gataccattc ctccccgctc
     1621 ttatcctgcc ctggcaccgg cggcaggtgc atataggaaa acacctgatc ccacggcaca
     1681 atgaatcctg ctccgtgcgc acagaatacg gaatccgccg tattctccag atcactctgg
     1741 gcatcatacc cgatctgcgc caccacttcc tccgtgttgt gacaggggaa atatcccttt
     1801 aaggcaaccg aaagcttccc ctctcccgcg gtgtatgccg tcacctcacg ctggtaattc
     1861 tgcatggcgg acacgggcgc acttcccaca atgcagccgt ctgcactgta ggcaatcgtt
     1921 cccttcatgc ggtcgatatc attcatggcg cgtccgaccg ctcctgccgg aagcgagagt
     1981 ctgaaatcat accacggctc cagcagcacc gtttccgcct gcatcagtcc ctgacgcagc
     2041 gcacgatagg ttgcctgcct gaaatcaccg ccctccgtgt gctttgtgtg cgcgcgtccg
     2101 gcaacgagtg tgatcttcat atccgcaagc gccgatcctg tcagcacacc gacgtgttcc
     2161 ttctctctta gatgggtgag caccagccgc tgccagttgc ggtcgagcac atcttcgctg
     2221 cagtccgtct caaatacgag cccacttccc ggctctcccg gttcaagcaa aagatgcacc
     2281 tcggcgtaat ggcggagcgg ttcaaaatgt cccacgccct ccaccgtacc tgcaatggtc
     2341 tccttgtata caatccttcc ggcgccaaat gtgacctgca caccgtagcg ctgacggatc
     2401 agttcgctca aaacctcgat ctgaacttcc cccatcacct gcacctggat ctcttttaag
     2461 gtctcatccc acacaatgtg aagctgcggt tcctcctcct caagttcccg cagtctggga
     2521 agcatgactg ccgggcttac ctcttccggc agtatgatct gataagtcag taccggctct
     2581 aacaccggca gcctggacgc tttctctgcg ccaagtccct cccccggaaa tgtgcgaagc
     2641 agacccgtga ccgcacagac gcctccggcg ccaacttcct ctgccgtctc atatttttcg
     2701 ccggaataaa tgcggatctg gttgatcttc tcctccccga tgacctgtct gacttttaag
     2761 cttccaccgg tcacttttaa gtaggtcagg cgatttccct gcgcatcccg tgcaatctta
     2821 aatacccgcg cgccgaatgt ctccccgtag gccgggatac tggcataccg gataaagtcc
     2881 accagaaacg ccttcacccc ctgcatcttg agcgcagatc cgaaatagca cggaaacagc
     2941 ttacgttccc ggatcagtct tttgatctgc tccgttccga ccgcttcccc tgccagatac
     3001 tgctctaaga cctgctcatc gcacaccgcc acattttccg caaaagccgc gctctcctga
     3061 tccgaaaaat ccacgcagtt ctcatgcagt tcctttttca attccaggag cagcgcctcc
     3121 tgatccgttc ccggctggtc catcttgttc acgaacaaaa acaccggcac ctcatacttc
     3181 gccagaagcc caaacagcgt cctcgtatgc gcctgcacac cgtccgcccc gctgatgaca
     3241 agaatcgcgt aatccaatac ctgaagcgtc cgctccatct ccgccgaaaa atccacatgc
     3301 cccggcgtat ctaaaagcgt gaccgtctcc tccggcagct ttaataccgc ctgcttggaa
     3361 aaaatggtga ttcctctctc cttctcaagc gcaaaggtat ccagaaacgc atccttgttg
     3421 tccacgcggc ccgccctgcg gatcattccg ctctcataga gcagtgcctc cgataaagtt
     3481 gtcttcccgg catctacatg tgccagaact gctgctgcaa tattcgcttt gtcactcacg
     3541 ttctccggtt ccttttccta caaactgatt cttctgtaac ggttgatgca tgcaaaaatc
     3601 tcctgtgccc tcggcattgc cagcccgcac ggcacatagc tgctctccgt caccgcggca
     3661 agtccgtgct cctccacata ctgcatcaat agggaaacgc tctctcttag ggtgcgcttc
     3721 ccgttgaata catggatctc ggcatacttt aaaaagtagg cgagcgccgc gagctgttcc
     3781 gtatccgcca gctgctcgac atagcgcaca tcgatcgtat cgcggttgat ggagatgccg
     3841 tcgcgtccca tcgtctttag cttcatgcgg gcatcttcct taaatgcacg gtccggccgc
     3901 accgcacgct caaaccccgg agccgcctgc ttcggcggtt tcacctccgg cagcgggaat
     3961 ctgcccgctt cctccttcgc aaatgcggtg atctcctgcg gtttgtaacg gttcatctgg
     4021 acaatgcagt ctgccacatg aaaataggag ccggaacttc ccgccaccag aatcgtggag
     4081 ataccgtact gttcatacag ctccctgata cggtcgataa acggcgtgat cggctcatcc
     4141 tcgcggttta ccaccctctg catcagctcg tcgcggatca taaaattggt tgcactcgta
     4201 tcctcatcga tgaggaacgt ctctgctccc gcctccatgg cctccaccac attggcagcc
     4261 tgggaggtgc ttccactcgc atcctccgtg cgaaaggcac tcgtatcctt tccgttcgga
     4321 agatcgttga tgaacatgga aatatccacc tttttgatgc ttcttccgtc ctccgcgcgg
     4381 atcttcatcg ccgtatcatc ggtaatgaca tactccctgc cgtccccggc gatatggttg
     4441 tacacgccaa gctccagcgc cttaagcagc gtggatttgc cgtggtatcc gccgccgacg
     4501 atgagtgtga ttcctttctt tattcccatg ccctgtatct ttcccgcatg cggcagcgca
     4561 aagcttacct tcatggattc cggcgccgta aacttcacgg cgtccttcat cggtctgccg
     4621 gacacgccgg attcacgcgg caggatggat gcatccgcca cgaatgccac cagcccctgc
     4681 tcctttaaat gatcccggat atactgctga tcctccgcga gatccgccgc acggtacacc
     4741 gccttttcat ccagcgcctt ataaaacagg ctgtgtgata cacattccgg aagaaacgta
     4801 aagaagattt tctccagttc tcttgcattg acggttctcc cgttggcagg gaatcccacc
     4861 tcaaagcgaa gcaccagatc gccctttgcc tcatccatct ggcatgcggt gcgctccaac
     4921 acttcctgtc ccgggcggct caccgccaga a
//
LOCUS       DBMDQJ010000087         4945 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6575_length_4945_cov_1.645954, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000087 DBMDQJ010000000
VERSION     DBMDQJ010000087.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4945)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4945)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4945
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6575_length_4945_cov_1.645954"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            182..2167
                     /locus_tag="ACZO9R_02730"
     CDS             182..2167
                     /locus_tag="ACZO9R_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912022.1"
                     /translation="MQKEKGIVSIKVKLLGVIVPVVIVSILVLVLIAYRTSAGLIESY
                     SENLLESSVENQASQIESWLEQNIGAFQIVKTTIENTKPDDKELQTMLDSYYNYNSNY
                     PEGLYIADETGKLWKASDSAMSESDPVNSVWYQEGLTRVNMAIGSSYVNSEGVSVISA
                     SGILNDGSGKMKVISADMTLDRISVIVNAFIEMKNAEAILVDKDTATILASRDSAKIS
                     TTLGSDGSSAFEQSVAAKIADRDYSFSTLDGNMTVFKEVSGTNWILVSYIPTSIVLAD
                     LANLRNLMILISVISILILCVVIERTTHVVIAPVRKLTNVIKALTDGDFTVSVKSSGN
                     DEIALMSRSVERFIASMKQMIASMGDISGKLGTQADASDSVSREMQSAANVQSQSMSE
                     LNMTVDQLSVSVNEIADNATKLAGVVADTKDDSVNVGNKMRETVEVSQKGREDMEHVG
                     EALENIRISIQNLEAAVNKVGTASGEIVEIVQLIGNIAEETNLLSLNASIEAARAGEA
                     GRGFAVVASEIGTLASNSTASVEHISKLIHEVNELVADAVRQAGDSADNINESSGLIH
                     TAIDTFDKIYDNIQQTSALIDQMVDKINKVDEVATNVAAISEEQAASSDEILATSESM
                     LTQAKGIAENSENVAKESRSLTESSIQLADQVKLFRI"
     gene            2181..3161
                     /locus_tag="ACZO9R_02735"
     CDS             2181..3161
                     /locus_tag="ACZO9R_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR0912023.1"
                     /translation="MKRLMSAVLVAALGVTCLGGCQAGSSGGDTAVNGKGVRIYVSLS
                     QADTFRNTLIDAAKKTAEASGAEIAVYDAENSIENQVAQIKQAVAEDYDVIMCGPVDA
                     DTALELEALAEGIPIVFYNSCPDASVLEPDKYIYVGSDEGVAGQYQAEYILDQMSDKD
                     EINVVLLKGPKNHSATKGRSEEFKNTLKASGKKINVVFEDNADWEQGIAEDYFDLFLQ
                     TGKQFDAVVCNNDTMALGVIDSCKKNGISGVPVLGIDATADGCAAIQAGDMAFTVYQS
                     GSGQGEAAVKAAIALGSGGTTQGMEGLSDDGLYVWVPFEKVDSSNVADYQ"
     gene            3219..3710
                     /locus_tag="ACZO9R_02740"
     CDS             3219..3710
                     /locus_tag="ACZO9R_02740"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HIR0912024.1"
                     /translation="MEYTHVTHTFGPVYDDASRILILGSFPSVKSREQQFYYGHPQNR
                     FWKVLAAVFEDTLPETIPQKKAFLLEHHIALWDVIASCDIAGSSDSSIRNVTANDMEV
                     ILKTADIRRICANGDTAYRLFSKYCLTAEMPEVKKLPSTSPANAAWNLERLTQCWAQE
                     LLL"
     gene            3743..4174
                     /locus_tag="ACZO9R_02745"
     CDS             3743..4174
                     /locus_tag="ACZO9R_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HIR0912025.1"
                     /translation="MKKNEMTWQVMLIEAVGIVSAIAYLGLQIYYGIAFHVNPVNLMM
                     NLVFMILVYVGLTLLAVYPERVNGLTREVCSGKIRQYTLRMVRMVKLVFVEGLLFTSV
                     CDALGKELKQGYSLIIVVLIAAIAVYYEGRIIHILKQNNKR"
     gene            4188..>4945
                     /locus_tag="ACZO9R_02750"
     CDS             4188..>4945
                     /locus_tag="ACZO9R_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912026.1"
                     /translation="MTKDMTEGAIPSLLVGFTIPLVLGNLFQLLYNAVDSMIVGKFVG
                     ETALAAVGTSSPLMTLAILFISGMCMGAGVLMGMHYGAKDYDVLERQISSTMIGGLMF
                     SVLLSAVCVFLTPWLLRLIRTQEEVLPIATGYLRIIFCGLIFTFIYNFYASTLRALGD
                     SRSPLYFLMISAALNVAGDLFFVVVLGWGSNGCAFSTVLSEALCCLLCGLYIKKKVKI
                     LCLGRLVFLFARIFLFGFFSLRLPFPVLFLLLLC"
BASE COUNT         1267 a         1028 c         1478 g         1172 t
ORIGIN      
        1 taatacgtgt atacaatttg agacgaaagg gcgctcggaa atggtataaa catgcgactt
       61 cagggcggga atccgtcgat agatgttgac agtaatatgc aaaaatgaga aaatattgac
      121 aagattatta tgcaattatc atagaatctg gggtatcaca agagaaaagg agttgaggca
      181 catgcaaaag gaaaaaggaa tcgtatcgat taaggttaag cttttaggtg tgatcgttcc
      241 ggttgtcatt gtcagcattc tggtgctcgt actgatcgcc taccgcacgt cggcagggct
      301 gattgagagc tattccgaga atctgctgga gtcatcggtg gagaatcagg cgtcgcagat
      361 tgagagctgg ctggaacaga atatcggggc gttccagata gtgaagacaa cgatagagaa
      421 cacgaagccg gacgacaagg aattacagac catgctggat tcctactata attataactc
      481 aaattatccg gagggacttt acatcgcgga tgaaaccgga aagctctgga aagcgtcgga
      541 ttcggcgatg agtgagtcgg atccggtcaa cagtgtctgg tatcaggagg gactgaccag
      601 agtcaatatg gcgatcggat cttcctatgt taattcagaa ggcgtcagtg tcatcagtgc
      661 atccggtatt ttaaatgacg gcagcggcaa gatgaaagtc atctctgcgg atatgacgct
      721 cgaccgcatt tccgtcattg taaatgcgtt tatcgagatg aagaatgcgg aggctatcct
      781 cgtggacaag gatacggcaa cgattctggc aagccgtgat tcagcgaaga tttccacgac
      841 gctcggcagc gacggcagca gtgcattcga gcagagtgta gcggcgaaga ttgcggatcg
      901 tgattacagc ttttccacat tggacggcaa tatgaccgtg ttcaaggagg tatccggaac
      961 taactggatt ctggtttcct atattccgac cagcattgta ctggcagacc ttgcgaacct
     1021 caggaatctg atgattctga tcagtgtgat ctccattctg attctgtgtg tagtgattga
     1081 gcgcacaacg catgttgtga tcgcacctgt ccggaagctg acgaacgtca tcaaggcgct
     1141 gacggacggc gactttaccg tatcggtcaa gagcagcggc aatgatgaga ttgcgcttat
     1201 gagccgcagc gtggagcgct ttatcgcatc catgaagcag atgattgcgt ccatgggaga
     1261 catttccgga aaactgggaa cgcaggcaga tgcgagcgac agtgtgtccc gagagatgca
     1321 gtcggcggcg aatgtccagt cccagtccat gagcgaactg aatatgacgg tggatcagct
     1381 gtccgtttcc gtcaatgaga ttgcagacaa tgcgaccaag cttgccggcg tggttgcgga
     1441 cacgaaggac gacagcgtga atgtcggcaa caagatgcgt gagactgtcg aagtatccca
     1501 gaagggacgc gaggatatgg agcatgtcgg tgaggcgctt gagaatatcc gcatttccat
     1561 ccagaatctg gaagcggcgg taaataaagt aggtacagca tccggtgaga ttgttgagat
     1621 tgtacagctg atcggaaata ttgcggagga gaccaacctc ctgtccttaa acgcttccat
     1681 tgaggcagca agagcgggcg aggcaggacg aggattcgca gtcgtcgctt ctgagatcgg
     1741 aacgcttgca agcaacagca ccgcatcggt agagcacatt tcaaagctca tccatgaggt
     1801 caatgaactt gtggcggatg cagtacgtca ggcaggcgac agcgccgaca atatcaatga
     1861 gagcagcgga ttgattcata cggcaatcga tacctttgat aagatttatg acaacattca
     1921 gcagacaagc gcgctgatcg accagatggt ggacaagatc aacaaggtag atgaagtagc
     1981 gaccaacgtg gcggcaattt ccgaggagca ggcggcaagc tcagatgaga ttcttgcaac
     2041 atcagagtcc atgctgacgc aggcaaaagg aattgcagag aacagtgaga acgtggcaaa
     2101 agaatccaga agcctgacag agtcgtccat acagctggcg gatcaggtta agctgtttcg
     2161 tatttaaggg ggtatcagat atgaagagat tgatgagtgc ggtactggta gctgctctgg
     2221 gagtgacatg cctcggaggc tgccaggccg gcagcagtgg aggagatacc gcggtaaacg
     2281 gcaagggcgt ccggatttat gtctcgctgt cacaggcgga tacattccgt aatacactga
     2341 ttgatgcggc gaagaagacg gccgaggcga gcggagctga gattgcggtg tacgatgcgg
     2401 agaattccat cgagaatcag gtggcgcaga tcaaacaggc ggttgcggaa gattacgatg
     2461 tgatcatgtg cggtccggta gacgcggaca cagcgctgga actggaggcg ctggcagaag
     2521 gcattccgat cgtcttttac aacagctgcc cggatgcatc ggtgctggag ccggacaaat
     2581 acatttatgt gggatcggat gagggagttg caggacagta tcaggcagaa tatattctgg
     2641 atcagatgag tgacaaggac gagatcaatg tggtactctt aaagggaccg aagaatcatt
     2701 ccgcaaccaa gggaagatcc gaggagttca aaaatacgct taaggcatcc ggcaagaaga
     2761 tcaacgtcgt gtttgaggat aacgcagact gggagcaggg aattgcagaa gattatttcg
     2821 atctgttcct gcagacaggc aagcagtttg atgcggtggt atgcaacaat gataccatgg
     2881 cgcttggagt catcgattcc tgcaagaaga atggcatatc gggagtaccg gttcttggaa
     2941 ttgacgcgac agcagatggc tgtgcggcaa tccaggcggg ggatatggca tttaccgtat
     3001 accagtcggg aagcggacag ggtgaggcgg ctgtcaaggc ggcaatcgca ctcggttccg
     3061 gaggaacgac gcagggaatg gaaggcctgt cggacgacgg actttacgtc tgggtaccgt
     3121 ttgagaaggt ggacagttcc aatgtggcag attatcagta atagagaggg aagagggcat
     3181 cagtgcggtc tgcactgatg ccgttttggt gtaagagaat ggaatatacg catgtgacac
     3241 atacgtttgg acctgtttat gacgatgcat cccggattct gattctggga tcctttccgt
     3301 ctgtaaagtc gagagaacag cagttttatt acggtcatcc gcagaaccgt ttctggaaag
     3361 tgctggcagc ggtctttgag gatacgctgc cggagacgat tccgcagaaa aaggcgtttt
     3421 tgttagaaca ccatattgcg ctgtgggatg tcattgcctc ctgtgacatt gcaggatcat
     3481 cggacagttc gatccgcaat gtgacggcga atgacatgga agtgatctta aagacggcgg
     3541 acatccggcg catctgtgcc aacggggata cggcgtaccg cctgttttct aaatattgtc
     3601 tgaccgcgga gatgccggaa gtaaagaagc tgccgtctac gagtcctgcc aatgccgcat
     3661 ggaatttaga gcggctgaca cagtgctggg cgcaggaact tttgctgtaa gaaaaagaga
     3721 gggaaaagga ggtgtgagac atatgaaaaa gaatgagatg acatggcagg tgatgctcat
     3781 tgaggcagtc ggtatcgtat cggcgatcgc ctatctcggg ttacagatct attacgggat
     3841 cgcgttccac gtcaatccgg tgaacctgat gatgaatctt gtgtttatga ttctggtata
     3901 tgtgggtctg accctccttg ccgtctaccc ggaacgtgtg aacggattga caagggaggt
     3961 atgcagcggt aagatcagac agtatacgct tcggatggtt cggatggtca agcttgtctt
     4021 tgtggaggga cttttattca ccagcgtctg tgatgcgctc gggaaagaat taaaacaggg
     4081 atacagcctg attatcgtgg tattgattgc agcgattgcg gtgtattatg agggaaggat
     4141 catacacatt ctgaagcaga ataacaaaag atagaaaggc atctgcaatg acaaaggata
     4201 tgacggaagg agcgataccg tctcttctgg taggttttac aattccactt gttctcggaa
     4261 atctgtttca gcttctctac aatgcggtcg acagtatgat tgtcggaaaa tttgtggggg
     4321 agacggcgct tgccgcggtc ggtacgagca gtccgctgat gacgcttgcg attctgttta
     4381 tcagtggcat gtgcatggga gcaggcgttc tgatgggaat gcactacggc gcaaaggatt
     4441 atgatgtcct ggagcggcag atcagttcga ccatgatcgg cgggctgatg ttttccgtcc
     4501 tgctttcagc cgtctgcgtt tttctgacgc cgtggctgct tcggctgata aggacgcagg
     4561 aggaagtgct tccgattgct acggggtacc tccgcattat tttctgcgga ctgatcttta
     4621 cgtttattta caacttttat gcgagcacat tgcgggcact tggcgacagc agatcgccgc
     4681 tttattttct gatgatcagc gctgcactca atgtggcggg agacctgttt ttcgtggtgg
     4741 tgctaggctg gggcagcaac ggatgtgcct tttcgacggt gctaagcgag gcactctgct
     4801 gtctgctgtg cgggctatat attaagaaaa aggtaaagat tctctgcctg gggcgcctcg
     4861 tcttcctgtt tgcgaggatt tttctttttg ggtttttcag cctccgtctg ccttttcctg
     4921 ttctgttcct cctgttgctt tgtag
//
LOCUS       DBMDQJ010000088         4883 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6673_length_4883_cov_1.595148, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000088 DBMDQJ010000000
VERSION     DBMDQJ010000088.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_4883_cov_1.595148"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(207..692)
                     /locus_tag="ACZO9R_02755"
     CDS             complement(207..692)
                     /locus_tag="ACZO9R_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912027.1"
                     /translation="MANSKRHKLVCCLMAMALLLSFFYVVPDPAGKAERGNAAACVAD
                     GQAALTGEMRHEGSWQNMMSAQTLLEARDAKPDACTDTFTMIHSVLDEVVMREQAGMR
                     SLLSKLLAVVVWVMLLVCVVCRILSRRYGCRMIYHWENIYYIHQTDGKKGDCLLNYLH
                     I"
     gene            895..1077
                     /locus_tag="ACZO9R_02760"
     CDS             895..1077
                     /locus_tag="ACZO9R_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR0912028.1"
                     /translation="MKANVIEGCISCGLCASVCPEVFRMNDENVSEVYHQPTPEQEDA
                     TQEAADGCPVSVIEIE"
     gene            complement(1115..2521)
                     /locus_tag="ACZO9R_02765"
     CDS             complement(1115..2521)
                     /locus_tag="ACZO9R_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0912029.1"
                     /translation="MKDGEVRDFGRRDKIGYMFGDFGNDFTFIFASSFLMVFYTKVLG
                     ISGGMVGTLFLVARFVDAFTDVTMGRIVDAAPPARDGKFRCWIRRMCGPVALSSFLMY
                     QTAMAQASMPWKIVYMYVTYLLWGSIFYTSINIPYGSMASAITTEASERTALSTSRSI
                     GATLAGLVIGIVTPLFLYTTDADGNQVVRGGGTFTVVAGIFSILALICYLVCYRMTTE
                     RVAVEREVDTESVSFAATLGAIVKSRALLGMIGAAVFLLLSQLLISTMNNYIYPDYFG
                     DARGISLFTLLSTLVILLVAAPLGVPVSRRFGKKEASVAGMLFSGVVFAVMYVLKLQN
                     MWLFLWLGAIGYLGLGFFNTVIWANITDVIDDQEVQTGHRDDGTVYAVYSFARKVGQA
                     LAGGIGGWALQVIGYEPAAAAQTDAVRRGLYTTATLVPAIGFFVVAAILWFVYPLDKR
                     RVEKNVEELKKKHLPAEH"
     gene            complement(2579..3721)
                     /locus_tag="ACZO9R_02770"
     CDS             complement(2579..3721)
                     /locus_tag="ACZO9R_02770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0912030.1"
                     /translation="MSTAAFLQINFIPAIALLMMRHNTRQTLSFSWRTRVLRTEMLLF
                     VLLLLCRTAILCNLGKGGFGPRILLKGLGVWYAFGLLACAYLCFLYVLDVAQDGNGQR
                     GIKCWILAIPALIGSAVLLSNPWTKAVYFINMVNYYKKGPAAYLIPAVGMLYLVVAFL
                     LAAFGALRAGSAQRRGELGKLAAAFLFPAAGILVEKIFSMDAAWAFTVAALLWVYLGI
                     QKGQVTQDGLTGLNNRRRLDQYMWELDERSGERETCCYLLMDVNKFKKVNDTYGHVTG
                     DEVLRLVAEQLKRTFGNMQAFLARYGGDEFVVIVKGKTQDEMDVIIHQTQENVNAIAW
                     GADTPWQLSISIGCAMRGEQGKQKSHELLALADERMYRQKKGIQDR"
     gene            complement(3749..>4883)
                     /locus_tag="ACZO9R_02775"
     CDS             complement(3749..>4883)
                     /locus_tag="ACZO9R_02775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0912031.1"
                     /translation="QLEIYLFPIVMLLAIGMNNWKREERTQGDRIFGFLPFLIICMMG
                     SDVTYHALQLYVHRFTVLYAGYLVYCFFLTAVPYAWLLYVNGKLSVRHNRRWTAAVCH
                     VMTGTAIAVVLLTVIIPWRLSTEGWGVHAVSYSLNHGSLPAKLVSIAMYLMGLILTAT
                     AYPKEVTKEGQKETRYLLEAGVFSLVGGMVQSFAESWRTGGPFVALAVLFIYLNAQNR
                     QITTDGLTGLNNRREFDAHLQRKIELCPEHEWGLLMIDVDDFKRINDELGHAVGDEAL
                     WHTADILRGVFGKDPVFLARYGGDEFAVLGDWFGQEQIEEAIARIQEGIDRFNKEGQL
                     PLQLSMSIGYAFWHEAGRRGENLIQQADERMYEEKQKKKRMRA"
BASE COUNT         1203 a         1545 c         1035 g         1100 t
ORIGIN      
        1 tcagaatcag atccgggcag atactggtgc tcatctccat gccggagcgt ccgctggtgg
       61 aagtataaag cctatagttc tcccgctgca gcaccctgcg gatcagataa aaaatacttt
      121 catcctcatc aatcagcaaa atctttactc tgtcgcttat ttggttttta tagttcactg
      181 aaactgcctc ctgtcattac gtttttctat atatgtaagt aattcagaag gcaatctcct
      241 ttctttccat ctgtctggtg tatatagtag atattctccc aatggtaaat catgcggcat
      301 ccgtaacggc ggcttagaat ccggcagaca acgcatacca gaagcatcac ccacacaacc
      361 accgccagca gtttggacag caggctgcgc atgcccgcct gctcccgcat gacgacttca
      421 tccagcacgg aatgaatcat cgtgaacgta tctgtgcagg catccggctt tgcgtccctt
      481 gcttccagca gcgtctgtgc agacatcata ttctgccagg aaccttcgtg cctcatctct
      541 cccgtcagag cagcctgccc gtctgccacg caggctgcag catttccacg ctccgccttg
      601 cctgccggat ccggtaccac ataaaaaaaa gacagcagga gagccatcgc catcagacag
      661 cataccaact tatgtctttt gctgttcgcc atttctccca ccatcctttc cgcatttgct
      721 aattattgta acatatattc tttttatatc aaacttgtat ttgtaaaatc atctttttct
      781 taatttctta gcggtttctt aacggctttt tcgtatatga cgcgtttaca ccggcaaccc
      841 taaacaggta accatcattc acctcccccg ggaggttcac agaaaggatg ccctatgaaa
      901 gcaaatgtga tcgaaggctg tatcagctgc gggctgtgtg ccagtgtatg tccggaggtc
      961 ttccggatga acgatgaaaa tgtatcggag gtctatcatc agccgacgcc ggaacaggaa
     1021 gatgccacgc aggaagccgc tgacggctgc ccggtgagcg tcatcgaaat cgagtaatcc
     1081 ccggcagaga aaaaacatct gcccgccgca ccagtcagtg ctcagcgggc agatgttttt
     1141 tcttgagttc ctccacgttt ttctccacgc gccgcttatc cagcgggtac acaaaccaca
     1201 gaatcgctgc cacaacaaaa aatccgatcg caggcaccag cgttgctgtg gtgtacaatc
     1261 cacgccgcac ggcatccgtc tgcgccgccg cagccggctc atacccgatc acctgcagcg
     1321 cccatcctcc gatgcctcct gccagcgcct gtccgacctt tctggcaaac gaatataccg
     1381 catagacagt tccgtcatcc cggtgcccag tctgcacttc ctgatcatcg atgacgtccg
     1441 tgatattcgc ccagatcacc gtattaaaaa agccaagccc gagatatccg attgccccga
     1501 gccacagaaa cagccacata ttctgtaatt tcagcacata catgactgca aatacaacac
     1561 cagagaacag catccccgcc acggacgcct cctttttccc aaatcgtctg gaaacaggca
     1621 caccgagcgg tgctgccacc agcagaatca ccagtgtgga cagcagcgtg aacagtgaaa
     1681 tgccacgggc gtccccaaag taatccggat agatatagtt gttcatcgtg ctgattaaaa
     1741 gctgggagag cagcagaaat accgcagctc cgatcatccc gagaagtgcg cggctcttca
     1801 caatcgcacc aagcgttgcc gcaaaactca cagattccgt gtccacttca cgctccaccg
     1861 ccacccgctc cgtagtcatg cggtaacaga ccagatagca gatcaacgcg agaatcgaga
     1921 aaatgcccgc aaccaccgta aatgttccgc cgccgcgcac gacctgattg ccgtctgcat
     1981 cggtcgtgta cagaaaaagc ggcgtcacga tgccgatcac aagccccgcc agtgtcgccc
     2041 caatcgaccg ggaggtagat aacgccgtcc gctccgatgc ctccgtcgtg atcgcagatg
     2101 ccatcgaacc atacggaatg ttgatcgatg tataaaaaat acttccccac aaaagataag
     2161 taacatacat gtagacaatc ttccacggca tagatgcctg cgccatggca gtctggtaca
     2221 tcaaaaaact cgacagcgcc accggtccgc acatcctgcg gatccagcat cggaactttc
     2281 catcccgcgc cggcggcgct gcgtcgacga tgcgtcccat cgtcacatcc gtgaatgcat
     2341 ccacaaatcg tgcgaccaga aacagtgtcc ccaccattcc accgctgata ccgagtacct
     2401 tggtatagaa taccatcaga aacgagctgg caaaaataaa ggtaaaatcg ttgccaaagt
     2461 caccgaacat gtagccgatc ttatcccgcc tgccaaagtc tctcacttct ccatccttca
     2521 ttgcactaac cctccgtctt ctgctcccat cggaacgtat gaagagagta tgcccggatt
     2581 atctgtcctg tatacctttt ttctggcggt acatgcgctc atccgcaagc gccagcagtt
     2641 catggctttt ttgctttccc tgctctcccc gcatcgcgca gccgatactg atcgacagct
     2701 gccacggggt atccgctccc caggcaattg catttacatt ctcctgtgtc tgatgaataa
     2761 tcacgtccat ctcgtcctgc gtcttacctt ttacaatcac aacgaattca tccccgccgt
     2821 agcgtgccag aaacgcctgc atattgccaa aggtgcgctt taactgctcc gccaccagcc
     2881 tgagcacctc gtcgccggtc acgtgaccgt aggtatcgtt tactttcttg aacttgttca
     2941 catccatcag cagatagcag caggtctccc gctctccgct gcgctcgtca agttcccaca
     3001 tgtactgatc cagtctgcgt ctgttgttaa gaccggtcag accatcctgc gttacctgac
     3061 ctttctgaat accaagatac acccacagca atgcagcaac cgtaaacgcc cacgcggcat
     3121 ccatggaaaa tattttttct accagaatgc ccgcagccgg gaacaaaaaa gcggcggcaa
     3181 gcttccccag ttctcccctg cgctgcgcac ttccggcacg gagcgcacca aacgcggcaa
     3241 gaaggaatgc caccacaagg tataacatgc ccacagcagg tatcagatat gccgctggtc
     3301 cctttttata gtaattcacc atattgataa aatagaccgc cttcgtccac gggtttgaga
     3361 gcagcacagc actgccgatc agtgccggta tcgccagaat ccagcatttt atccctctct
     3421 gtccgtttcc gtcctgcgcc acatccagca catacaaaaa gcacaggtag gcgcatgcca
     3481 aaaggccgaa cgcataccac acccccagcc cctttaacaa aatccttgga ccaaatccgc
     3541 cttttccgag attgcacaaa atcgctgtgc ggcacagtaa aagcagtaca aacagcagca
     3601 tctccgtgcg cagcacgcgt gtcctccacg aaaaagacag cgtctgcctg gtattatggc
     3661 gcatcatgag cagcgcaatc gccggtatga aattgatctg taaaaatgct gccgtactca
     3721 cgcagtccgc ctccccatgc accgcgctct atgcgcgcat acgtttcttt ttctgtttct
     3781 cctcatacat ccgctcatcc gcctgctgta tcaggttctc cccgcgcctt ccggcttcat
     3841 gccagaacgc gtagccgata ctcatggaca gctgcagcgg aagctgtccc tctttgttaa
     3901 accggtcgat accttcctgg atccgcgcga tcgcttcctc aatctgttcc tgcccaaacc
     3961 agtctccgag caccgcaaac tcatcccctc catagcgtgc gagaaatacc ggatctttgc
     4021 cgaacacccc ccgcagaata tccgctgtat gccagagcgc ctcatcgccc accgcatgtc
     4081 ccagttcatc gttgatccgc ttgaaatcat ccacatcaat catcagaagt ccccactcgt
     4141 gctccgggca caattcgatc ttccgctgca gatgtgcgtc aaactccctg cggttattca
     4201 gtccggtcaa cccgtctgtc gtgatctgcc ggttctgcgc attcagataa ataaacagta
     4261 ctgccagtgc cacaaaaggt cctcccgtgc gccagctctc cgcaaagctc tgcaccatcc
     4321 cgcccacaag gctgaacacg cctgcctcaa gcagatagcg cgtctctttc tgcccttcct
     4381 tggtaacctc cttcggatac gccgtcgctg tcagaataag tcccatcaga tacatcgcaa
     4441 tacttaccag ctttgccggc agactgccgt gatttaagct gtaggacacc gcatgcacgc
     4501 cccatccttc cgtgctcagt ctccagggaa tgatcaccgt aagcagcacc acagcaatag
     4561 cggttcctgt cataacatgg cataccgcag ccgtccaccg ccggttatgc cgcacagaaa
     4621 gctttccgtt cacatacaaa agccaggcat acggtactgc tgtcaggaag aagcagtaca
     4681 ccagataccc ggcatacagc accgtaaacc gatgtacata aagctgcagt gcatgatacg
     4741 tcacatcact gcccatcatg cagataatca gaaacggaag aaacccgaaa atccggtctc
     4801 cctgcgtccg ttcctcccgc ttccagttat tcattccgat ggcaaggagc atcactatag
     4861 gaaacaggta gatttcaagc tgt
//
LOCUS       DBMDQJ010000089         4878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6681_length_4878_cov_1.394808, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000089 DBMDQJ010000000
VERSION     DBMDQJ010000089.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6681_length_4878_cov_1.394808"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="ACZO9R_02780"
     CDS             complement(<1..650)
                     /locus_tag="ACZO9R_02780"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005343276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR0912032.1"
                     /translation="MSEVKIATRESYGNALVELGGEHENLVVLDADLAAATKTGMFKK
                     AFPDRHIDCGIAEANMTGVAAGMSTCGFVPFVSTFAMFAAGRSYEQVRNSIGYPHLNV
                     KIGATHAGISVGEDGATHQCNEDIALMRAIPGMVVINPADDIEARAAVKAAYEYVGPV
                     YLRFGRLAVPVINDGADYKFEIGKGVTLREGSDCSIIATGLCVSEALAAADTLAEQG"
     gene            complement(638..1474)
                     /locus_tag="ACZO9R_02785"
     CDS             complement(638..1474)
                     /locus_tag="ACZO9R_02785"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR0912033.1"
                     /translation="MDKLALQKMAVEVRKGIVSSVHSAKAGHPGGSLSAADIFTYLYF
                     EEMNIDPKDPKKEDRDRFVLSKGHTAPGLYSALANRGYFPVEDLLTLRHTGSYLQGHP
                     DMKHIPGVDMSSGSLGQGLSAACGMALAGKMKQQDYRVYALCGDGEIEEGQIWEAAMF
                     AGFRKLDNLCVIVDNNNLQIDGPIDEVCSPYPIDKKFESFNFHVINIDGNDFDEIKRA
                     FDEARATKGMPTAIIAHTVKGKGVSYMENSVGWHGKAPNDEEYAVAMEDLRKAGEALC
                     QK"
     gene            complement(1551..2204)
                     /gene="fsa"
                     /locus_tag="ACZO9R_02790"
     CDS             complement(1551..2204)
                     /gene="fsa"
                     /locus_tag="ACZO9R_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR0912034.1"
                     /translation="MKFFIDTANVEDIRKANDMGVICGVTTNPSLIAKEGRDFNEVIK
                     EITTIVDGPISGEVKATTVDAEGMIKEGREIAAIHPNMVVKIPMTVEGLKAVKVLSAE
                     GIKTNVTLIFTANQALLAARAGATYVSPFLGRLDDISTAGIDLIQDIVQIFDNYGLET
                     EIIAASIRNPIHVTDCALAGAHIATVPYKVIEQMTKHPLTDAGIEKFQADYRAVFGD"
     gene            complement(2325..3821)
                     /gene="araA"
                     /locus_tag="ACZO9R_02795"
     CDS             complement(2325..3821)
                     /gene="araA"
                     /locus_tag="ACZO9R_02795"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR0912035.1"
                     /translation="MLATKNYKFWFCTGSQDLYGDECLAHVAEHSKIIVAKLNESGVL
                     PYEVVWKPTLITNELIRKTFNEANVDEECAGVITWMHTFSPAKSWILGLQEYRKPLLH
                     LHTQFNEEIPYDTIDMDFMNENQSAHGDREYGHIVTRMGIERKVVVGHWSDVNVQERI
                     ASWMRTAVGVIESSHIRVMRVADNMRNVAVTEGDKVEAQLKFGWEVDTYPVNEIAEAV
                     AAVSESDTNALVDEYYDKYDILLEGRDPQEFRRHVAVQAQIEIGFERFLDEKNYQAIV
                     THFGDLGALKQLPGLAIQRLEEKGYGFGAEGDWKVAAMVRLMKIMTAGKKEAKGTSML
                     EDYTYNFVKGKEGILEAHMLEICPSIADGPISIKCQPLSMGDREDPARLVFTSKEGSG
                     IATSLIDLGNRFRLIINDVDCKKTEKPMPKLPVATNYWTPQPDLYTGAEAWILAGGAH
                     HTAFTYDLTAEQMGDWANAMGIEAVYIDKDTKIREFKKDLMLGNLFYR"
     gene            complement(3962..>4878)
                     /locus_tag="ACZO9R_02800"
     CDS             complement(3962..>4878)
                     /locus_tag="ACZO9R_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HIR0912036.1"
                     /translation="AGTLTEEGAKLLDPSGRLEAGIPLCPPEGDAGTGMVATNSVAVR
                     TGNVSAGTSVFAMVVLEEDLKAVHEELDMVTTPSGDTVAMVHCNNCTSDLNAWVGLFR
                     EFAENFGINVDMNELFGKLYNKALEGDKDCGGLLAYNYFSGEPVTGANEGRPLFVRKP
                     DAHFNLANFMRTHLYAALATLKIGLDILLKEEKVKVDRIYGHGGLFKTKGVGQGILAA
                     AMDAPVAVMETAGEGGPWGMALLASYMLQKADGESLEQYLSEKVFGGESGSVMEPDPA
                     DVAGFDAYIKAYKAALAAEKAAVEAMPL"
BASE COUNT          992 a         1473 c         1138 g         1275 t
ORIGIN      
        1 ccctgctctg ccagcgtatc cgctgctgcc agtgcttccg agacacaaag tccggttgca
       61 atgatggagc agtcgcttcc ctcacggagc gtcacgccct tgccgatctc aaacttataa
      121 tccgcgccgt cgttgatgac cggaaccgcc agtctgccaa agcgcagata caccggtccc
      181 acgtactcgt aggcagcctt cactgccgcg cgcgcctcga tgtcgtccgc cgggttgatg
      241 acaaccattc ccgggatcgc acgcatcagg gcaatatcct cgttgcactg atgggtcgcg
      301 ccgtcttccc cgacggaaat tccggcatgt gtcgcaccga tcttcacatt cagatgcgga
      361 tatccgatgg aattgcgcac ctgctcgtaa gaacgtcctg ccgcaaacat ggcgaaggtg
      421 ctcacaaacg gtacaaatcc acatgtggac attccggctg ccacgccggt catattcgcc
      481 tctgcgatac cgcagtcaat gtggcggtcc gggaatgcct tcttgaacat tccggtcttg
      541 gttgccgctg cgagatccgc atccaggaca accagattct catgctctcc tccaagctcg
      601 accaaagcat tgccgtagct ctctctcgtt gcaatcttta cttctgacac aatgcttcac
      661 ctgcctttct aagatcctcc atcgccactg catattcctc gtcgttcggc gctttaccgt
      721 gccagccgac gctgttctcc atgtaggaga caccttttcc ctttacggtg tgcgcaataa
      781 tcgcggtcgg cattcccttc gtggctctcg cctcatcaaa cgcccgcttg atctcatcga
      841 aatcattgcc gtcgatgttg atgacgtgga aattaaagct ctcgaacttc ttgtcgatcg
      901 ggtacggcga gcagacctcg tcgatcggtc cgtcgatctg caggttgttg ttgtccacaa
      961 tcacgcagag gttatccagc ttgcggaaac ctgcgaacat ggcagcctcc cagatctgtc
     1021 cctcctcgat ctcgccgtcg ccgcaaagtg catacacacg gtaatcctgc tgtttcatct
     1081 tccctgccag agccatgccg cacgctgcgg aaagtccctg tcccagagaa ccggaagaca
     1141 tatccacgcc cggaatgtgc ttcatatcag gatgtccctg taaatacgat ccggtatggc
     1201 gcagggtcaa aagatcctcc accggaaaat aaccgcggtt tgccagtgcc gagtaaagtc
     1261 ccggcgccgt gtgtcccttg gacagcacaa aacggtcgcg gtcctccttc ttcggatcct
     1321 tcggatcaat attcatctct tcaaaatata aataggtgaa gatatctgct gctgagagtg
     1381 atccgcccgg atgtcccgcc ttcgcgctgt gcacggacga tacaattccc ttgcggactt
     1441 caacagccat cttctgaagt gccagcttat ccatagttcc cccttcatgc attctttgtc
     1501 attgctactg caaaactttg tcatcaaata ccgcagagca tcttcggtaa ttagtcacca
     1561 aatacagctc tgtagtccgc ctggaatttc tcaattcccg catcggtaag cggatgcttg
     1621 gtcatctgct caataacttt gtacggaacc gttgcgatat gtgcgcctgc taatgcacag
     1681 tctgtcacat ggatcgggtt gcggatcgat gcagcgataa tctcggtctc aagtccgtag
     1741 ttgtcgaaga tctgcacgat gtcctggatc agatcgatac ctgccgtgct gatgtcgtcg
     1801 agtctgccaa ggaacggaga tacataggtt gcgcctgcgc gcgctgcaag aagcgcctgg
     1861 tttgctgtga aaataagcgt cacattcgtc ttgattccct ctgcggacag cactttgact
     1921 gcctttaatc cctctacggt catcgggatc ttgactacca tgttcggatg gatcgctgcg
     1981 atctcacgtc cttctttgat catgccttct gcatccacag tggttgcttt tacctcgccg
     2041 ctgatcggac cgtcaacgat cgtcgtaatc tccttgatga cctcgttgaa atctctgccc
     2101 tctttggcaa tcagagacgg gtttgtggta acgccgcaga tcactcccat atcatttgcc
     2161 ttgcggatgt cctctacgtt tgctgtgtca ataaaaaact tcatattcct ctccctttct
     2221 gcgcttgtgc gcgcacccta tatgtgtatc cgggtctgcc ccggtaccat tctcatttac
     2281 cgggggatgc cgttttgaca tccccgtatg cttcttattg ttttttagcg gtaaaacaga
     2341 ttgccaagca tcagatcctt cttgaactcg cggatctttg tatccttgtc aatataaact
     2401 gcctcgatgc ccatggcatt tgcccagtcg cccatctgct ctgcggtgag gtcgtaggta
     2461 aatgcggtgt ggtgtgcgcc gcctgccaaa atccatgcct ccgcaccggt gtagagatcc
     2521 ggctgcggtg tccagtaatt cgttgctacc ggaagcttcg gcatcggctt ctctgtcttc
     2581 ttacagtcca catcgttgat gatgagacgg aaacggttgc cgagatcgat caaggatgtc
     2641 gcgataccgc ttccttcctt ggaggtaaat accagtctcg cagggtcttc tctgtcaccc
     2701 atggaaagcg gctggcactt gatggaaatc gggccgtctg cgatggacgg gcagatctca
     2761 agcatatgtg cctcgaggat tccctctttg ccttttacga aattgtaggt gtaatcctcc
     2821 agcatggaag taccctttgc ctccttcttg cctgctgtca taatcttcat caggcgcacc
     2881 atcgctgcca ctttccagtc accctctgcg ccaaagccgt agcctttttc ctcgaggcgc
     2941 tggattgcaa gtcccggaag ctgctttaag gcaccgagat caccgaagtg tgttacgatt
     3001 gcctgataat tcttctcgtc caggaaacgc tcgaaaccga tctcgatctg tgcctgtacg
     3061 gctacatggc gtctgaactc ctgcggatca cgtccctcta ataaaatgtc atatttgtca
     3121 taatactcat ctaccaaagc gttggtatcg gactcggata cagctgcaac tgcctccgca
     3181 atctcgttga ccggataggt atccacttcc cagccgaact tcagctgtgc ttcgacctta
     3241 tccccctcgg taacagctac gtttctcatg ttgtctgcca cacgcatcac gcggatatgg
     3301 ctgctctcga tcacgccgac tgctgtgcgc atccaggatg caatgcgctc ctgcacattg
     3361 acgtcgctcc agtgtcccac gactaccttg cgctcgattc ccatacgtgt cacgatatga
     3421 ccgtactcac ggtctccgtg tgccgactgg ttctcgttca tgaaatccat gtcgatggtg
     3481 tcgtaaggaa tctcctcatt gaactgggta tgtaaatgaa gcagcggctt tctgtactcc
     3541 tgtaatccga gaatccagga ctttgccggg gaaaatgtgt gcatccatgt gatgacgcct
     3601 gcgcactcct catctacatt tgcctcgttg aaggtcttac ggatcagttc attggtgatc
     3661 agtgtcggct tccataccac ttcatacgga agcacgccgg actcgtttaa ctttgccacg
     3721 atgattttgg aatgctctgc cacatgtgcg aggcactcgt ctccgtaaag atcctgtgat
     3781 ccggtgcaga accagaactt gtaatttttt gttgctaaca taatgttccc tccatgaaat
     3841 atatttatgt ttcagcagta actgctgtgt aaatcccaat ctgtaagcag cggcagcatc
     3901 tgccgctgcc cccgtatctg tctataacgg catcgtccag gctgccgcct tcgtgcctgt
     3961 cttataacgg catcgcctcg actgccgcct tctctgctgc aagcgccgct ttgtatgcct
     4021 tgatgtaagc gtcaaatccc gcaacatccg ccggatccgg ctccatcacg cttccggact
     4081 ccccgccgaa gactttttcg gagaggtact gctccaggct ctcgccgtcc gctttctgca
     4141 gcatgtagga tgccagcagc gccatgcccc acgggccgcc ctcgccggct gtctccatca
     4201 ctgccaccgg cgcatccatc gctgcggcca aaattccctg tccgacaccc ttggtcttaa
     4261 agagtccgcc gtgtccgtag atgcggtcca cttttacttt ttcttccttc agcagaatat
     4321 ccagaccgat ctttaaggtt gcaagcgctg catacagatg tgtccgcatg aaattggcga
     4381 gattaaaatg tgcatccggc ttgcgcacga acagtggccg tccctcgttc gctccggtca
     4441 ccggctctcc cgagaaatag ttgtatgcca gaagtccgcc gcagtccttg tcaccctcca
     4501 gcgccttatt gtaaagcttt ccgaaaagct cgttcatatc gacattgatt ccgaagttct
     4561 ccgcaaactc ccggaacaga ccgacccagg cgttcagatc cgaggtacag ttgttgcagt
     4621 gcaccatcgc cacggtatcg ccggacggtg tggtcaccat atccagttcc tcgtgtaccg
     4681 cctttaagtc ttcctccaaa accaccattg caaacacgga tgttccggca gatacatttc
     4741 cggtgcgcac agccaccgaa ttcgttgcca ccataccggt accggcatct ccctccggcg
     4801 ggcagagtgg aattccggct tccagcctgc cgctcggatc aagaagcttt gctccctcct
     4861 ctgtcagtgt tccagcat
//
LOCUS       DBMDQJ010000090         4842 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6730_length_4842_cov_1.642480, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000090 DBMDQJ010000000
VERSION     DBMDQJ010000090.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6730_length_4842_cov_1.642480"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /gene="gatB"
                     /locus_tag="ACZO9R_02805"
     CDS             <1..943
                     /gene="gatB"
                     /locus_tag="ACZO9R_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR0912037.1"
                     /translation="AEEVIAYLEKLRLIIQYLGASDCKLNEGSMRADVNLSVREAGAA
                     EFGTRTEMKNLNSFKAIARAIEGERARQIELIEEGKKVIQETRRWDDNKESSHAMRSK
                     EDAQDYRYFPEPDLVPVVISDEWIERVKARQPEMRDEKLIRYQDEFGLPKYDAEILTG
                     DKSFADLFEAAAAICGKPKKVSNWIMTETIRLLKENEMDPDQIRFSPEHLAKLVDLAD
                     AGSVTNTVAKEVFEKVFKEDIDPEAYVKEKGLLTVNDEGALRETVAKVIAENPQSVED
                     YHNGKEKAIGFLVGQTMKAMKGKANPALVNQILKELL"
     gene            complement(1505..1942)
                     /locus_tag="ACZO9R_02810"
     CDS             complement(1505..1942)
                     /locus_tag="ACZO9R_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HIR0912038.1"
                     /translation="MLNISGIHEGFVLDHIKAGMSLQIYHDLKLDELDCTVAIIKNAK
                     SSKMGKKDILKVECPVEALDLDILGFIDHNITVNVIKDDKIYEKKELHLPKQVKNVIT
                     CRNPRCITSIEQELDQIFLLTDEEKEVYRCKYCEEKYRNPNRR"
     gene            complement(1969..2889)
                     /gene="pyrB"
                     /locus_tag="ACZO9R_02815"
     CDS             complement(1969..2889)
                     /gene="pyrB"
                     /locus_tag="ACZO9R_02815"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HIR0912039.1"
                     /translation="MRHLMSPLDFSVDELDKLLDLANDIEKYPQKYAHACEGKKLATC
                     FYEPSTRTRLSFEAAMLNLGGSVLGFASADSSSAAKGESVSDTIRIISCYADICAMRH
                     PKEGAPLVASQKSRIPVINAGDGGHQHPTQTLTDLLTIRSLKGRLGNLTIGLCGDLKF
                     GRTVHSLISALIRYPGIRFVLISPEELRIPSYIREDVLAANNIPFTEVERLEDAMPDL
                     DVLYMTRVQKERFFNEEDYVRLKNFYILTKAKMDLAKEDMLVLHPLPRVNEISVEVDD
                     DPRAAYFRQAQYGVYVRMALILTLLDIRVD"
     gene            3167..3577
                     /locus_tag="ACZO9R_02820"
     CDS             3167..3577
                     /locus_tag="ACZO9R_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HIR0912040.1"
                     /translation="MAEETNFSQGEAEEVKYQERKRLLFFGLPWTFTKYTISQDMITV
                     DEGLIKVEENDCYMYKVQDVKLTATLPERIFGMGTIICYTGDVTDKELRLSHIKHAKE
                     IKNYLLKASEAARIKRRTLNMQDIGAGIADDVDL"
     gene            complement(3643..4041)
                     /locus_tag="ACZO9R_02825"
     CDS             complement(3643..4041)
                     /locus_tag="ACZO9R_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0912041.1"
                     /translation="MRGRNFLICGLTGWCMEILFTSAGSLARHDGRLIGQTSLWMFPI
                     YGMAACIGPVYAKIKKLPALLRGSIYTVGIFSFEYLSGSLLRRHHFCPWDYSSARANV
                     NGLIRLDYAPLWLGAGLLFERILVHANRLT"
     gene            4216..>4842
                     /locus_tag="ACZO9R_02830"
     CDS             4216..>4842
                     /locus_tag="ACZO9R_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR0912042.1"
                     /translation="MKHLTYEHVQGSQSREITDICYHSGNVREGSLFVCLKGLHNDGH
                     DYLQGALEAGAAAVVVAAENVVPLDNDALLYTEQFGVRIAEIIDRWQVSVVIAADTRH
                     ALAELAAAFFDYPAAKLRMVGITGTKGKTTTAYLLSAILRESGHKTGMIGTIAIDTGD
                     GVLPAVHTTPEAYEIQKYLAQMVDNGCDTCVMEVSSQGLKMQRVEGIFF"
BASE COUNT         1286 a         1084 c         1331 g         1141 t
ORIGIN      
        1 cgcggaggag gtcattgcct atctggaaaa attgaggctg atcatccagt atctcggtgc
       61 atcggactgc aaattaaacg aaggttccat gcgtgccgac gtcaacctgt ccgtcaggga
      121 ggcgggagcc gctgagtttg gaacacgtac cgagatgaaa aacctgaact ctttcaaggc
      181 gatcgcgaga gccatcgaag gcgagcgcgc ccgccagatc gaattgatcg aggaggggaa
      241 aaaggtcatc caggagaccc gccgctggga tgacaacaag gaatcctccc acgcgatgcg
      301 atccaaggag gacgcacagg actaccgtta tttcccggag ccggatctgg ttccggtggt
      361 catcagcgac gagtggattg agcgcgtgaa ggcacgccag ccggagatgc gggatgagaa
      421 gctgatccgc tatcaggatg agttcggtct gccaaaatac gatgcggaga tcctgaccgg
      481 agacaaatca tttgcagatc tgtttgaggc agcggccgcg atctgcggaa agccgaaaaa
      541 agtctccaac tggatcatga cggagacaat ccgcctgtta aaagaaaatg aaatggatcc
      601 ggatcagatc cgcttttcac cggaacatct tgcaaagctg gtggatcttg cggatgccgg
      661 ttccgtcacc aacaccgtgg caaaggaagt cttcgagaaa gtctttaaag aggacattga
      721 tccggaagcc tatgtgaagg aaaaggggct tttgacagtc aatgacgagg gagcactccg
      781 tgagacggtg gcgaaagtca ttgcggagaa tccgcagtcg gtggaggatt accacaatgg
      841 aaaagaaaaa gcgatcggct ttctggtcgg acagacgatg aaagcgatga agggaaaggc
      901 aaatcctgca ctggtgaacc agatcttaaa agaattgctg taagcggtca gtgtcttttg
      961 tagtgataag cagtactatt agaaatagat acctgaaagt tccagataac catacggcag
     1021 caaatttttt gaagcaaaat tcaagtattt cttccatgcc gcggctggta tttcattccg
     1081 tgtctcttcc acgtttggac tctcccggag ggtgtccgac aaatcagaga caatgagcgg
     1141 actttctagc ggcgctaatg cgcgctccct cataaacatt ataaaatcgc gcggactgaa
     1201 actcggagac ttctgctatt aaaaacatgg cagaaatctc ctcagacatc agtccgcgcg
     1261 attaggtttt actcggaagc gtgcaaagcg ccgcacgaaa gctcctaaaa ttgtctcatg
     1321 atttgcggac accgtccgag gagtgctcca aacttggaaa gacacggatc atcatgaatg
     1381 ccagccttcg cttggttaga aataccggat tttgcggtca aaatttgccg ccgtatggtt
     1441 atctggaact ttcaggtatc tgtttctaat aatactgctt atcccttaaa aaggcacagt
     1501 cggtttatct gcggttggga ttccggtatt tttcttcgca gtatttgcag cggtagactt
     1561 ccttttcttc atcggtcagg aggaagatct ggtcaagctc ctgctcgatg gaagtgatac
     1621 agcgcggatt cctgcaggtg atgacattct tgacctgctt tggaagatgc agctcctttt
     1681 tctcatagat cttgtcgtcc ttgatgacgt tgactgtgat attgtgatcg atgaagccga
     1741 ggatgtcgag atccagcgct tcgaccgggc attccacctt caagatatcc ttcttgccca
     1801 ttttgctgct cttcgcgttt ttgatgatgg caaccgtgca gtcgagctcg tcgagcttca
     1861 ggtcgtggta gatctgcagg ctcataccgg ctttgatatg gtctaataca aaaccctcgt
     1921 ggattccact gatatttaac atgtatctgt cctttctgcc gattttattt aatcgaccct
     1981 gatgtctaag agtgttagga tcagcgccat gcggacatag acgccgtact gtgcctgcct
     2041 gaagtaggcg gctctcgggt cgtcgtcgac ctcgacggag atctcgttga ctctcggaag
     2101 cggatgaagc accagcatat cctccttggc gagatccatc ttcgctttgg tcagaatgta
     2161 gaaattcttt aagcggacgt agtcttcctc gttgaagaag cgttccttct gtacacgggt
     2221 catgtataaa acatccagat ccggcatggc gtcttccaga cgttccacct cggtgaacgg
     2281 gatattgttc gctgcaagga catcctcgcg gatgtagctt gggattctaa gctcttccgg
     2341 ggagatcagc acgaaacgga tgcccggata gcggatcagt gcagagatca aagaatgtac
     2401 ggtgcgcccg aactttaagt cgccgcagag tccgatcgtg agattgccaa ggcgtccttt
     2461 taaagaacgg atggttaaca gatcggtaag agtctgggtg ggatgctgat gaccgccgtc
     2521 gccggcattg ataacgggaa ttcgggattt ctgggaggca acaagaggtg caccttcctt
     2581 tggatgacgc atagcacaga tatctgcata gcaggaaata atacgaatgg tatcggaaac
     2641 actttcacct ttggctgccg aactggaatc agcactggcg aaacctaaaa cagaaccgcc
     2701 gagatttaac atggcagctt caaaacttaa tctggtacgg gtgcttggct cataaaaaca
     2761 ggttgccagc tttttccctt cgcatgcatg ggcatacttc tgtgggtact tctcgatatc
     2821 gttggcaaga tccaaaagct tgtccaactc atcgacggag aagtccaatg ggctcattaa
     2881 atgacgcata aaatcctcct tgtattgacc ggaaacggca gaacaaatct gtctgtctcc
     2941 aggcattttt tctatcatat aataatcact ctgcgaattc aaggggaaaa atatttacgc
     3001 acgaatcgca aaatggcgga ctttttgtgt gatatttcaa aagattggag ccgttttctg
     3061 atatttggtt gcgcgaaaaa gaaaaatgaa gtaaactaca ggtacgcaga aaagaatgca
     3121 aaaagtttcg cctggggcgg aacatacaag gaaaggaaga acagacatgg cagaagagac
     3181 aaattttagt cagggagaag cggaggaggt aaagtatcag gagcgcaaac ggttactttt
     3241 ctttggactt ccatggacat ttaccaaata tacgatcagt caggatatga ttaccgtcga
     3301 tgagggattg atcaaggtgg aagagaatga ctgctacatg tacaaagtgc aggatgtcaa
     3361 gctgacagcc acactgccgg agcggatttt cgggatggga acgatcatct gctacaccgg
     3421 agacgtgaca gacaaggaac ttcgcctgag tcatatcaag catgcaaagg agattaagaa
     3481 ttatcttctg aaggcatccg aggcggcgcg catcaagaga cgcacattaa acatgcagga
     3541 catcggtgca ggcattgcag atgatgtgga tctgtaggaa aagccgctgc ttttacaggg
     3601 cgcaggagtg cgtgccggga aagggcgcca tgcgcggtct ggctatgtga gccggtttgc
     3661 gtgcacgaga atgcgctcaa agagcagacc cgcgccgagc cacagcgggg cataatccag
     3721 ccggatcagc ccgttgacat ttgccctggc gctgctgtag tcccaggggc agaaatgatg
     3781 ccgccttaag agagaaccgc ttaagtactc gaatgaaaaa atacccactg tatagatgct
     3841 gccgcgcaga agtgccggca gctttttgat ttttgcatag accggaccga tacaggcagc
     3901 cataccgtag atggggaaca tccacaggga ggtctgaccg atcagacggc cgtcgtgtct
     3961 tgcgagggag cctgcggagg tgaacagaat ttccatgcac cagccggtca gtccgcagat
     4021 gagaaaatta cgtccgcgca tatgagagat tcctccggtt ttcgtttgtg tttcagtatt
     4081 tccggttcct gacaaaatat gtacattttc tgaaattaaa atgaaataaa tcgggataac
     4141 ttgtaaaacc ttaagatctg gcatataata aaaaaagtga attcggcaga ggatgacatg
     4201 cggctgaatc aattattgaa acatcttaca tatgaacatg tgcagggaag ccagagccgt
     4261 gagataacag acatctgtta ccattccggg aatgtcaggg aaggctccct atttgtttgc
     4321 ctaaaagggc tacataacga cgggcatgac tatctgcagg gagcgctgga ggccggggcg
     4381 gcagcagttg tggttgcggc ggagaatgtg gttccgctgg acaatgatgc gcttttgtac
     4441 acggaacagt ttggtgtgcg gatcgcggag atcatagacc ggtggcaggt ctccgttgtg
     4501 attgcggcgg ataccagaca cgcgctcgca gagcttgcgg cagctttttt tgattatccg
     4561 gcggcaaagc ttcgtatggt tggaattacg ggaaccaagg ggaagacaac gacggcgtat
     4621 ctgctttctg ccattcttcg agagagcggg cataagaccg gaatgatcgg aacgatcgca
     4681 atcgataccg gagacggcgt tcttccggcg gtacatacga cgccggaagc ctacgagata
     4741 cagaagtatc tggcgcagat ggtggacaac ggctgtgata cctgcgtgat ggaggtctct
     4801 tcccaggggc tgaagatgca gcgggtggag gggatatttt tt
//
LOCUS       DBMDQJ010000091         4824 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6755_length_4824_cov_1.443997, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000091 DBMDQJ010000000
VERSION     DBMDQJ010000091.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4824)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6755_length_4824_cov_1.443997"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            190..1143
                     /locus_tag="ACZO9R_02835"
     CDS             190..1143
                     /locus_tag="ACZO9R_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912043.1"
                     /translation="MIEINHLVKKYGEHVAVDDLTLTVEPGKIYGFLGPNGAGKSTTM
                     NMITGYLGATSGEVKINGHDIFADPQEAKKCVGYLPEIPPLYTEMTVREYLDFVAELK
                     GLDKKVRKIQTEEIMELTHTKEVSERLIRNLSKGYRQRVGFAQAIMGYPDVIILDEPT
                     VGLDPKQIIEVRELIKELGKKHTVILSSHILTEISAVCDHVFILSKGKLVASDSTEHL
                     LERMSGAQEIALLVKEKEQRMEELLKELPGVAEVSREPGEAAEEVLFHVTAERGEDIR
                     EALFHACAKEEIPVLEMHTETKSLEDVFLELTGADGKGEQA"
     gene            1140..3257
                     /locus_tag="ACZO9R_02840"
     CDS             1140..3257
                     /locus_tag="ACZO9R_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HIR0912044.1"
                     /translation="MIAILKREWKSYFQNITGWLFIAAVLALYGLYFLAYNLRAGYPY
                     VSYTLSAISFIMLIAVPILTMRSMAEERHSRTDQLVLTAPVSLGKMIFGKFLAMVGVF
                     TVAVAVIAVTPLVLAAFGTVPMGENYVAVLGFWLYGCTCIAVGMLASALTESQVIAAV
                     VAFAFLFLGYMMNSITGLIGDHLITKVLGAYDLYTPLQSFMSGCLDLTGVVYFVSVTA
                     LCLFLTCQCVQKRRWSMTTKKLTTGMFSVAMIVVGFAVAVAVNMVVKEMPSSWTAVDA
                     TSTKLYSLTDTTKDYVKNLSQDVTIYVLSSEKSADSTLAETLQRYEDLSKHLNVVYKD
                     PAKSPNFYQQYTDSAPSQNSMIVVSDARSRVVDYSDIYEYSYDYSTYSSSVDGYDAEG
                     QLTSALQYVTKADSELPVIYEITGHGENTLSGGFSEAVEKANMTVSQLTLLTEDAVPD
                     DAAAIIINGPTSDFSEDDAAKVKEYLQGGGRALITCNFEYQDLPNFASVLEAYGIERV
                     AGIVMENSASACYRSTPYYLLPEIESTAYTSSVTGGYIFAPYSEGLSYPEESEDITYT
                     PLLVTSDQAVSKTDVANAETSELEDGDIAGPFTIALTAEQDVDDDNTMKLVVFGSTEM
                     LTDNADSIVSGRNVSMFSDALGQLAGDDGESTGVIPVKEYTLGTITVTSLGTLIGGLA
                     ATVILPILLIVTGVVIWAVRRKK"
     gene            3257..3901
                     /locus_tag="ACZO9R_02845"
     CDS             3257..3901
                     /locus_tag="ACZO9R_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025598.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HIR0912045.1"
                     /translation="MEKQKKQFLGMLLVLAVLAAAYGGIHVYNQKQEEKEASEEEAEK
                     LTVVDFKTDDVTAFSYVLGGQVYAYTKTDGEWTWDGDISLTLDTSQIDAMLDAVTGLT
                     AESEITDGEDLAQYGLENPSGIITLTTADGTTTLQLGDKNAVTGQYYLKVAESDKIYL
                     VSRDLSGTFSKTPQELLKEEETDEPESVDVTEGVEAAVGVDVTESAASEDSTEE"
     gene            complement(4456..4701)
                     /locus_tag="ACZO9R_02850"
     CDS             complement(4456..4701)
                     /locus_tag="ACZO9R_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912046.1"
                     /translation="MKKVKRILAAAGAILLLLLYASTLVCALSSSENFMNLLMVSIYA
                     TVIIPVLLWAYTLIYKLVHKSSEEDAEQKEPPQGQGK"
BASE COUNT         1241 a         1035 c         1409 g         1139 t
ORIGIN      
        1 ccatctgcat ttccacatga aatccccaag tgtgtttctt ataatattac ttatcacccc
       61 aaagaggtct atgcttattg caacaattcc tttgggtttg ttttcacaga attatcacag
      121 aatgattccc ttttgaacac atttctttcg taggctagat acggaaacag gaaaaaggag
      181 gaaatggttt tgattgaaat caatcatctg gtcaaaaaat atggggaaca tgtggcggtg
      241 gatgatctga cgcttacggt ggaacccggg aaaatctatg gttttctcgg accgaatggt
      301 gccggaaaat ccacgacaat gaatatgatt acaggatacc ttggcgcgac gagcggcgag
      361 gtgaagatca acggtcacga tatttttgcc gatccgcagg aggcaaaaaa atgcgtgggg
      421 tatctgccgg agataccgcc gctctatacg gagatgacgg tgcgggagta tctggatttc
      481 gtggcagagc tgaaaggtct ggataaaaag gtaagaaaaa tacagacaga agagattatg
      541 gagctgacac acacgaagga agtgagtgag aggctgatcc gcaatctctc caaaggatat
      601 cggcagcgcg tcggctttgc acaggcgatc atggggtatc cggatgtcat tatactggat
      661 gagcccacgg tgggactgga tccgaagcag atcatcgaag tgcgtgaact gattaaggag
      721 ctcgggaaaa agcacaccgt gattttgagt tcccatattc tgacagagat ttcggcggtc
      781 tgcgatcatg tgtttatcct gtcgaaggga aaacttgtgg cgagcgattc gacggaacat
      841 ctgctggagc ggatgtcggg agcacaggaa atcgcgcttc tggtaaaaga gaaagagcag
      901 cgcatggagg aactcttaaa ggaacttccg ggtgtggcgg aagtgagcag agagccggga
      961 gaagcagcgg aggaagtcct gtttcatgtg acggcggagc ggggcgaaga tatccgtgag
     1021 gcattgtttc atgcctgcgc aaaagaagag attccggttc tggaaatgca cacggagaca
     1081 aaatcgctgg aggatgtttt ccttgaactt acaggagcag acgggaaagg agaacaggca
     1141 tgattgcaat tttaaaaaga gagtggaaaa gttattttca gaatatcaca ggatggctgt
     1201 ttatcgctgc cgttctggca ctgtacggtc tgtacttttt agcgtataat ctgcgggcgg
     1261 gatatccgta tgtgtcgtac acactttcgg cgatctcgtt tatcatgttg attgccgttc
     1321 cgattctcac catgcgttcc atggcggaag agcggcacag cagaactgac cagctggtac
     1381 tcacagcgcc ggtttcgctt ggaaagatga tttttggaaa atttttagcc atggtgggcg
     1441 tttttaccgt tgccgtggcg gtgattgcgg taacgccgct tgttctggct gcctttggca
     1501 cggtgccgat gggagaaaac tatgtggcgg ttttgggctt ctggctgtac ggatgtacct
     1561 gcatcgcagt cggtatgctg gcgtcggcgc tgacggagag tcaggtaatt gcagcggtcg
     1621 tggcgtttgc attccttttt ctgggatata tgatgaacag catcacggga ttgatcggcg
     1681 accatctgat cacgaaggta ctgggcgcat atgatctgta cacaccgctt cagagtttta
     1741 tgagcggctg tctggatctg acgggcgttg tgtattttgt cagtgtgacg gcattgtgtc
     1801 tgtttttgac ctgccagtgt gtgcagaaga gacgctggag catgacgacg aaaaaactga
     1861 ccacaggaat gttttccgtg gcgatgattg tggtgggatt tgcggttgcg gttgcagtca
     1921 acatggtggt aaaggagatg ccatcctcct ggacggcggt agatgcaact tccacaaagc
     1981 tgtacagtct gacggatacc acaaaggatt atgtgaaaaa tctgtcgcag gacgtcacga
     2041 tctatgtgct ttcgtccgag aagtcggcgg acagtacgtt ggcggagaca ttgcagcggt
     2101 atgaagatct ctccaaacat ttaaatgtgg tttacaaaga tccggcgaag agtccgaatt
     2161 tttatcagca gtatacggac agtgcgccga gccagaacag tatgatcgtg gtgtcggacg
     2221 cgagatcgcg cgtggtggat tacagcgaca tctatgaata ttcttatgac tattctacat
     2281 attcaagctc tgtggacggt tatgatgcgg agggacagct gaccagcgcc ctgcagtatg
     2341 tgacgaaggc agacagtgaa cttccggtca tctacgagat taccggtcat ggagaaaata
     2401 ccctttccgg cggtttttcg gaggcggttg agaaagccaa tatgacggtg tcgcagctga
     2461 cattgctgac ggaagatgcg gttccggatg atgcggcggc aattatcatc aacggaccga
     2521 catcagactt ctcggaggat gatgccgcaa aggtgaaaga atatttgcag ggcggcggaa
     2581 gagcgctcat cacctgcaac ttcgagtatc aggatctgcc gaattttgct tcggttctgg
     2641 aggcgtacgg cattgaacgg gtagctggca ttgtgatgga aaacagcgca tcggcatgct
     2701 accggagcac accgtattat ctgctgccgg aaatcgagtc gacagcctat acttcatctg
     2761 tgacgggcgg gtacattttc gcaccgtaca gcgagggact gagctatccg gaggaatcgg
     2821 aagatattac ctacacaccg ctgcttgtca caagtgatca ggcggtgtcc aagacggatg
     2881 tggcaaatgc ggagacgtct gagctggaag acggggacat tgcaggaccg tttacgattg
     2941 cgctgacggc agaacaggat gtggatgacg acaacacgat gaaactggtg gtgtttggct
     3001 ccacggaaat gctgaccgac aatgcggact cgattgtatc gggaagaaat gtgtccatgt
     3061 tctcagatgc gttaggacag cttgccggtg acgacggaga gtcgacgggt gtcattccgg
     3121 tcaaggagta tacgcttgga acgattacgg ttacgtcact cggaacgctg atcggcggac
     3181 ttgcggcaac ggttatcctg ccgattctgc tgattgtgac cggtgttgtg atctgggcgg
     3241 taaggagaaa gaaataatgg agaaacagaa aaaacagttc cttggcatgc tgcttgtcct
     3301 cgcagtgctt gcagcagcct atggaggaat tcatgtatac aatcagaaac aggaggaaaa
     3361 agaggcgagc gaggaggaag cagagaagct tacagtcgtc gattttaaaa ccgatgatgt
     3421 gacggcattc tcgtacgtgc tgggcggtca ggtttatgcc tacacgaaaa cggacggaga
     3481 gtggacctgg gacggggata tttcactcac cctcgacacg tcacagatcg acgccatgct
     3541 ggatgcggtc acgggcctta cggcggagag tgagattaca gacggcgagg atctggcgca
     3601 gtatggtctg gaaaatccgt cggggatcat taccctcacc acggcagacg gcacgacgac
     3661 gctacagctc ggagataaaa atgcagtgac cgggcagtat tacctgaagg tggcagagag
     3721 cgataagatc tatctcgtgt cgcgggatct ctccggcact ttctccaaga caccgcagga
     3781 attgttaaag gaggaagaga cggatgagcc tgagagtgtg gacgtgactg agggcgtaga
     3841 ggcggctgtg ggcgtagacg tgaccgagag cgccgcgtcc gaggacagta cggaagagta
     3901 gaggacggat caaaagagcc actgcacggg cagggagatg gactccgtaa taagagaaaa
     3961 acgatagatg ttccagatac aaaggatgcg gacattctaa acacaaaatg cggaatttct
     4021 aaccctgccc gccgaaacat ccctgatgat ccatgtcttt ccaagcttgg agcactctac
     4081 agggtgtgtc cgcaagccgg cagacaattt taggactttc gtgtgtccct ttggacaccg
     4141 ctgaacaaaa cataaccgcg cgaagccgct gtctgagtcg gcatttgccc tgtgttcaat
     4201 agcagatgcc ggcgagttcg gttcgcacgg ttttatattg actgtgaagc ggtgcccatt
     4261 agggacacta gaaagtccgc tcattgtctg cggttgccgg acacaccctg tggattgtcc
     4321 aagcgtgaca aagacacgga attcgatgtc ccggcgggca gggaagaaat tctctcattt
     4381 tgtttcaaag aatccccgca tccgtttgta ttctggaaca ttctgtcggt ttttctctta
     4441 tcctgaaatt tgtatctatt ttccctgccc ctgcggcggc tccttttgct cggcgtcctc
     4501 ttcggacgac ttgtgcacga gcttatagat gagcgtgtac gcccacagca gaaccggaat
     4561 gatgacggtc gcgtagatgg agaccatcag cagattcata aaattctcgc tggaacttaa
     4621 agcgcagacg agcgtggatg catacagcaa aagcagaaga atggcgccgg ctgcggcaag
     4681 aatacgtttc acttttttca tggtaacctc atttctaaac agccttctgg tttacggctg
     4741 tacctttatg gtagatggaa aaggcggttc cgtcaagcag aaaaaattta cacgaattgt
     4801 acaaacttta taaaaaccat ataa
//
LOCUS       DBMDQJ010000092         4775 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6842_length_4775_cov_1.338254, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000092 DBMDQJ010000000
VERSION     DBMDQJ010000092.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4775)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4775)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4775
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6842_length_4775_cov_1.338254"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="ACZO9R_02855"
     CDS             <1..226
                     /locus_tag="ACZO9R_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912047.1"
                     /translation="EDKIILTFNYKDGSKTITLAEVEGSDLSVLGAVEQEKSGRQDLN
                     LFKLLLPAVEQETERRIRREVFGFYGRFRE"
     gene            complement(347..646)
                     /locus_tag="ACZO9R_02860"
     CDS             complement(347..646)
                     /locus_tag="ACZO9R_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HIR0912048.1"
                     /translation="MIQNDDILNMNFYKKEKFTGSYQGMRYLIQKDQEEDAEDENVKH
                     DIFRVTIWPGPYNFASTADDLKSSAVFPFTPEGKEQVVGWLNEQWSARRTEWPGR"
     gene            955..1866
                     /locus_tag="ACZO9R_02865"
     CDS             955..1866
                     /locus_tag="ACZO9R_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912049.1"
                     /translation="MGKYIVKRVLLAIISILIVSAITFFVMNIIPGGPFNKEKATSAE
                     AQKVLEERYNLDKPIPEQYVIYMNNLLHGDWGVSLHSGRDIWGEISSRFKVSAKLGGM
                     AALVAIVAGLVLGSIAALTRNHWPDRLIVFFTTLGTAMPSFVLATLLLLVFCLKLGWF
                     PVWSSTSPNYVLPIIALSVYPMAYITRLTKTSMLDSLNQDYIRTARAKGVPGWKVIFK
                     HALRNALIPVITYVGPMVAYILTGSMVVENIFTIGGLGSSFVTSITNRDYTMIMATTI
                     FLAILMVVANLLTDIAYKFVDPRITFE"
     gene            1891..>2426
                     /locus_tag="ACZO9R_02870"
     CDS             1891..>2426
                     /locus_tag="ACZO9R_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912050.1"
                     /translation="MELKHRQILSAQVDLSKFNKANFEKATDEEKKQQDVMGESTTFF
                     KDGMRRLRKNPLAMGSIIVLVLILLTIIIAPHICPYKYSEIISVNGVRDKSAANLGPF
                     EYSKLEAKYMNETGEKLFPHIFGTDSLSRDYFIRVIYGTRVSLSVGVVASIMVLIIGL
                     LYGSIAGYFGGKVDLIMMR"
     assembly_gap    2427..2436
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2437..2970
                     /locus_tag="ACZO9R_02875"
     CDS             <2437..2970
                     /locus_tag="ACZO9R_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912051.1"
                     /translation="DIIYSLPDMLIIILLSVVFNETLKPLITGTVLEKLGTNMISMFI
                     VFGLLYWVSMARLIRGQILSIKNNEYVLAARCIGTKNARILRRHILPNCLSVIIITTA
                     LQVPSAIFTESYLSFLGLGVSAPLTSLGSLANDARAGMQSYPLRLVIPAIAICLIVLA
                     LNLLGDGLRDAFDSKLN"
     gene            2983..3990
                     /locus_tag="ACZO9R_02880"
     CDS             2983..3990
                     /locus_tag="ACZO9R_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912052.1"
                     /translation="MSEENKYILEVKDLHTSFFTDAGEVKAVNGISFNLEPGKTLGIV
                     GESGSGKSVTAYSIMQILAETGKITGGEVRFHGEDITKWNSKQMQKFRGEKCSIIFQD
                     PMTSLNPVFTVGSQLIEAILLHTDKNKKEAWARAVEMLTLVGVNEPESRMKQYPHELS
                     GGMRQRVMIAMALVCEPDILIADEPTTALDVTIQAQILELMQDLQKKLGMAIIMVTHD
                     LGVIASMCDEILVMYGGRVCERGTADDIFYRPAHEYTKGLLRSIPKADNMNEKLVPIG
                     GTPINLLQMPAGCAFCPRCDAAMKICLSEKPEELRISDTHLASCWMNIKELYEAQEEA
                     E"
     gene            3990..>4775
                     /locus_tag="ACZO9R_02885"
     CDS             3990..>4775
                     /locus_tag="ACZO9R_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912053.1"
                     /translation="MSEEKNLLEVSHLKQYFPVHQGFKTIPLKAVDDISFAIKPGETL
                     GLVGESGCGKTTVGRTLLRLYQPTAGRIVFDGKVLFDSGEQYDENGKLIVDANGKPVL
                     GKKVDVDMLPYRRQMQMVFQDPYSSLNPRMTVEDIIGEPLDVHKLYANKKERREKILN
                     LMELVGLNAEHAMRYAHEFSGGQRQRIGIARALAVNPKFIVCDEPVSALDVSIQAQVI
                     NMFEELQEKLGVAYLFIAHDLLVVRHISKRIAVMYLGKIVEVAD"
BASE COUNT         1291 a          998 c         1242 g         1234 t
ORIGIN      
        1 tgaggacaag ataatcctta ctttcaacta taaggacggt tctaagacca tcacactggc
       61 ggaggttgag ggttcggatt tatccgtact cggtgcagta gagcaggaga aaagtggaag
      121 acaggacttg aaccttttca agctcctgtt acccgcagta gagcaggaga cagagcgcag
      181 aatacggcgt gaagttttcg ggttttacgg gcgtttccgt gaataggaat agaataagaa
      241 aagccatgta cgaagatgta tacttcgtat gtggcttttt ttgatgctgt gttacagtgc
      301 agtgataatg aatcagcgag atgctcatat aaagaaagag actgattcaa cggccgggcc
      361 attcggtgcg gcgcgcactc cactgttcgt tcagccagcc gaccacctgc tctttgccct
      421 ccggcgtaaa cggaaagaca gcagaacttt tcagatcgtc cgcggtgctg gcaaaattat
      481 aaggaccggg ccagatggtg acgcgaaaaa tatcatgttt tacattttcg tcctcggcat
      541 cctcctcctg atctttttgg atgaggtaac gcattccctg ataactgccg gtaaattttt
      601 cctttttata aaagttcata ttcagaatat catcattttg aatcatgatg gatctgcctt
      661 ctttctgtaa gagataatgt gattgaagaa agtataacaa atttctaaaa aaaaagacag
      721 agtatttatg tgggaaaaac acttgtattt actgggcgta cagaaaaagg aggtaatatc
      781 catgtatttt tgtatacaat tagcagttga caaatgatag tgagcgagta taatacactt
      841 atattatttt atcggattaa agtgacattg cattagagat acaaacagat aaaagaacgc
      901 aatgatagag cgataaaatg agaaagaatt ttcaagaagc aggagagaaa aaaagtggga
      961 aagtatatcg taaaacgagt tttactggcg attatatcca tcctgatcgt cagtgccatt
     1021 acattctttg tgatgaacat tatcccgggc ggtcctttca ataaggaaaa ggcaacgagt
     1081 gcggaggcac agaaagtcct ggaagagaga tataatctgg acaaaccaat cccggagcag
     1141 tatgtgattt atatgaataa tcttcttcat ggtgactggg gcgtatcttt acatagcggc
     1201 cgtgatatct ggggcgagat ttcttcccgt tttaaggtat cggcaaagct tggcggcatg
     1261 gcggcattgg tggcaatcgt ggctgggctt gtacttggaa gcattgcggc gctgacgcgt
     1321 aatcactggc cggaccggct gatcgtattt tttacaacac ttggtacggc gatgccgtcc
     1381 ttcgtgcttg caacactgct tcttctggtg ttctgcttaa agctcggctg gttcccggta
     1441 tggtcgtcca cgagtccgaa ttatgtactg ccgatcatcg cgctgagtgt ttatccgatg
     1501 gcgtatatca cacgtctgac aaagaccagt atgctggatt ccctgaacca ggattacatc
     1561 cgtaccgcga gagcgaaggg tgttccgggc tggaaagtca ttttcaaaca tgcactgcgc
     1621 aatgcgctga ttccggtaat cacttacgtg ggaccgatgg ttgcatacat tttgacaggt
     1681 tccatggttg ttgagaatat ttttactatc ggaggactgg gatcgtcttt cgtaacaagt
     1741 attaccaacc gtgactatac gatgattatg gcaacgacaa ttttcctcgc aattctgatg
     1801 gttgtggcaa acctgctgac cgatattgca tataagttcg tcgatccaag aatcacattt
     1861 gagtaaagta cgaaaggacg gatagaagta atggagttga aacatagaca gattctgtcg
     1921 gcacaggtag atctgagcaa attcaataag gcaaatttcg agaaggcgac agatgaggaa
     1981 aagaagcagc aggatgtcat gggtgagtct accacatttt tcaaggatgg tatgagacgc
     2041 ctgcgcaaga atccgcttgc catgggaagt atcatcgtac tggtattgat tcttctgacc
     2101 atcatcatcg caccgcatat atgtccttac aagtattccg agattatctc ggtcaacggt
     2161 gtgcgtgaca aatccgcagc gaacctagga ccgtttgaat attccaagct tgaggcaaag
     2221 tatatgaatg agaccggcga gaagctgttc ccacacatct tcggaacaga cagcttaagc
     2281 cgtgactact ttatccgtgt aatctacgga acgagagtca gcctgtcggt cggcgttgtg
     2341 gcatcgatca tggtattgat catcggtctg ctttacggtt ccatcgcagg atattttggt
     2401 ggaaaagtcg acctcatcat gatgcgnnnn nnnnnngata ttatttattc cctgccggac
     2461 atgctgatca tcattctgct gtcggtagtg tttaatgaga cgttaaaacc gctcattacc
     2521 ggaacggtgc tggaaaagct gggaacgaac atgatctcca tgtttatcgt attcggactt
     2581 ctgtactggg taagtatggc gcgtctgatc cgaggccaga tcctttccat taagaataac
     2641 gagtacgttc tggcagcaag atgcatcggt acaaagaatg ccagaatcct gcgcagacat
     2701 attctgccga actgtctgtc cgttatcatt attacgacag cacttcaggt gccgagtgca
     2761 atttttaccg aaagttatct gagtttcctt ggccttggtg tatctgcacc gcttacttcc
     2821 ctgggaagtc tggcaaacga cgccagagcc ggcatgcagt cctacccact tagattggtc
     2881 attcctgcaa tcgccatctg cctgatcgta ctggcattga acttactggg tgatggtctt
     2941 cgtgatgctt tcgactcgaa attgaattaa ggagataagg atatgagcga agaaaataaa
     3001 tatatattag aagtaaaaga ccttcatacc agtttcttta cagatgcagg tgaggtaaag
     3061 gctgtcaatg gtatttcctt taacttagag ccgggaaaga ccctcggaat cgtaggtgag
     3121 tccggttccg gtaaatctgt taccgcatat tccatcatgc agattctggc ggagaccggt
     3181 aagatcaccg gcggcgaggt aagatttcac ggtgaggata ttaccaaatg gaacagcaag
     3241 cagatgcaga agttccgtgg agaaaaatgc tccatcattt tccaggatcc gatgaccagc
     3301 ttaaacccgg tatttaccgt tggaagccag ctgattgagg caatattatt acacacggat
     3361 aagaataaaa aagaggcgtg ggcgcgcgcg gtggagatgc tgacactggt cggcgtcaat
     3421 gagccggaaa gccgtatgaa gcagtatccg catgagctgt ccggtggtat gcgtcagcgt
     3481 gtcatgatcg ccatggcact tgtctgtgaa ccggatatcc tgattgcaga tgagccgaca
     3541 acggcacttg atgtgacaat tcaggcacag attctggaac tgatgcagga cctgcagaaa
     3601 aagcttggaa tggcgattat catggtaacc catgatctcg gtgtcattgc aagtatgtgt
     3661 gatgagattc ttgtcatgta cggtggacgt gtctgcgagc gcggtacagc ggatgatatt
     3721 ttctatcgtc cggcacatga gtataccaag ggactgcttc gttccatccc aaaggcagac
     3781 aatatgaacg aaaagctggt gccgatcggc ggtacaccga tcaacctgct gcagatgccg
     3841 gctggctgtg cattctgccc acggtgtgat gcggcgatga agatctgtct ttccgagaaa
     3901 ccggaagaac ttcgcatcag cgatacccat ctggcaagct gttggatgaa catcaaagag
     3961 ttatatgagg cacaggagga ggcagagtaa tgagtgagga aaagaacctt cttgaggtaa
     4021 gtcatttaaa acagtatttt cctgtgcatc agggatttaa gacgattccg ctaaaagcgg
     4081 tggatgatat ttcatttgca atcaagccgg gcgagacact tggtctggta ggagagtcgg
     4141 gatgcggcaa gacgactgtc ggcagaacgc tgcttcgtct gtaccagccg accgccggac
     4201 gcatcgtgtt cgatggtaag gtgctatttg acagcggcga gcagtatgat gagaatggca
     4261 agctgatcgt cgatgcaaac ggcaagccgg tgcttggcaa gaaagtggat gtggacatgc
     4321 ttccttaccg cagacagatg cagatggtat tccaggatcc gtattcatcc ttgaacccgc
     4381 gtatgacggt cgaggacatc atcggagagc ctctggatgt tcataaatta tatgcgaata
     4441 aaaaagagcg ccgtgagaaa atcttgaacc tcatggagct ggttggactg aatgccgagc
     4501 atgccatgcg ttatgcacat gagttttccg gcggacagag acagcgtatc ggtattgcaa
     4561 gagcactcgc ggtaaacccg aagtttatcg tctgtgacga gccggtatcc gcactggatg
     4621 tgtccattca ggcgcaggtc atcaacatgt ttgaagagct gcaggaaaag ctcggcgtgg
     4681 cgtatctgtt catcgcgcac gatctgctcg ttgtgcgtca catctccaag cgcattgcgg
     4741 tcatgtatct cggcaagatc gtcgaggttg ccgat
//
LOCUS       DBMDQJ010000093         4752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6885_length_4752_cov_1.555579, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000093 DBMDQJ010000000
VERSION     DBMDQJ010000093.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4752
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6885_length_4752_cov_1.555579"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..125
                     /locus_tag="ACZO9R_02890"
     CDS             <1..125
                     /locus_tag="ACZO9R_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HIR0912054.1"
                     /translation="QNFSIFSPLSKVVLIFDMLAGRLELFPLMILFMPSTWRKK"
     gene            379..1236
                     /locus_tag="ACZO9R_02895"
     CDS             379..1236
                     /locus_tag="ACZO9R_02895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0912055.1"
                     /translation="MWIADNWKDYEVIDCSKGEKLERWGDYLLVRPDPQVIWDTPKTE
                     RGWRKMNGHYHRSAKGGGEWEFFDLPQQWTIHYGKLTFNLKPFSFKHTGLFPEQAANW
                     DWFSEKIRSAGRPIKVLNLFAYTGGATIAAAAAGASVTHVDASKGMVTWAKENAVSSG
                     LGDAPIRWIVDDCVKFVEREIRRGNHYDAIIMDPPSYGRGPKGEIWKIEDAIYPLVQL
                     CEQLLSDDPLFFLINSYTTGLQPAVLSYMMNTVLKKHGGHIAADEIGLPVSSNGLVLP
                     CGASGRFER"
     gene            1392..2921
                     /locus_tag="ACZO9R_02900"
     CDS             1392..2921
                     /locus_tag="ACZO9R_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIR0912056.1"
                     /translation="MAQFVKLVPENLKALREVNPRLMSYNVEFAEVTGGTFWKAYTPE
                     QVAGTEEFHVAPSADGIAAMYKDLMQVYAPIDLYNEKLRSLAKELGTAWVRVSGTWAT
                     KTYYDFDNATGGIAPEGYLNVLTKEQWIGVLDFVKAIDAKLMISVANCPGLHSAEEPW
                     NPSEAEKIFRLSKEHGVPIDGAEFANEPNMMEDTGFPAGYTPADYHRDQDLFFAWVRA
                     NYPECICIGPSSVGEGLTINGGDDNSKSGGIEQLVRENCSTTDLLEDTKEPLDVFSYH
                     YYNGVSERLASVMPSGHWLADTAHTEAYLDVALNCARTYAPLRDKYCPGGEMWVTESG
                     DAGGGGDTWASTYLDVFRTLNELAGFAAITDGVIFHNTLASSDYGFLAREVFDPRPNY
                     FAVLLWNRLMGTTVYDTAEPIREGAHVFAHSRKDGKEGVVYLVINNSLTEATTVELPK
                     SAERYTLAGEGGNVRAAVMTLNGKPLVLGAGNSLPELTPAAQEAGTVTLAPATCTFFV
                     L"
     gene            complement(3551..3970)
                     /gene="atpC"
                     /locus_tag="ACZO9R_02905"
     CDS             complement(3551..3970)
                     /gene="atpC"
                     /locus_tag="ACZO9R_02905"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HIR0912057.1"
                     /translation="MNTFSLKVIACDRVFFDGRCEQVVLPLHDGEKAIQAHHENMVFA
                     VEIGEIRITDETGEEIVGVTGTGFAQIINNRAMVIVDTCESPEEIDVRRAEEAKERAQ
                     EQLRQKQSIQEYYRSKASLARAMSRLKVGKKDRPTGY"
     gene            complement(3967..>4752)
                     /gene="atpD"
                     /locus_tag="ACZO9R_02910"
     CDS             complement(3967..>4752)
                     /gene="atpD"
                     /locus_tag="ACZO9R_02910"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR0912058.1"
                     /translation="TALVFGQMNEPPGARMRVAETGLTMAEYFRDEEHQNVLLFIDNI
                     FRFTQAGSEVSALLGRMPSAVGYQPTLATEMGELQERIASTKNGSVTSVQAVYVPADD
                     LTDPAPATTFAHLDATTVLSRKIVEQGIYPAVDPLESNSRILEEDVVGKEHYETARRV
                     QEILQKYTELQDIIAILGMEELSEEDKLVVYRARKIQKFLSQPFHVAENFTGVPGKYV
                     PLKETVRGFKAIIDGEMDEYPEAAFFNVGTIDDVIERAKEMGA"
BASE COUNT         1060 a         1274 c         1221 g         1197 t
ORIGIN      
        1 cgcagaattt ttcgatcttc tcgccgctct ccaaggttgt gctgattttt gacatgctcg
       61 ccggcagact ggagcttttc ccgctcatga ttctgttcat gccgtccacc tggcgtaaaa
      121 aataacgtgc agccggcaat ttgggctgcg cagttttgaa ccggtagata tttttctacc
      181 ggttttttat tggcttttta tgggcttttt cctgctccgg atgctgtatc cgcatccgtt
      241 aatgcctgcg ccgcgccgtg gcgaaggctt tttctctgcc cgcaggcaga cgtttttact
      301 ttttccttgc cctctgctgg cttgtggtat atatttgtta tagttttaaa ttagaagtac
      361 acaggaatgg agattgctat gtggattgca gataactgga aagattacga agtcattgac
      421 tgctcaaaag gagaaaagct cgagcgctgg ggagattatc ttctcgtccg tcccgacccg
      481 caggtcatct gggacacgcc gaagaccgag cgcggctggc gcaaaatgaa cggccattac
      541 caccgcagcg ccaagggcgg cggggagtgg gaattttttg atcttcccca gcagtggacc
      601 atccattacg gcaagctgac tttcaactta aagccgttca gttttaagca caccggactg
      661 tttccggagc aggctgccaa ctgggactgg ttttccgaaa agatccgcag cgccggacgt
      721 ccgatcaagg tgttaaatct gttcgcctac actggcggtg ccacgattgc cgccgctgct
      781 gccggtgcca gcgtcacgca tgtggatgcc tccaagggaa tggtgacctg ggcgaaggaa
      841 aacgccgtct cttccggtct tggtgatgcc ccgatccgct ggattgtgga cgactgcgta
      901 aagtttgttg agcgcgagat ccgccgcggc aaccactatg atgcgatcat tatggacccg
      961 ccttcctacg gaagaggacc caagggtgag atctggaaaa tcgaggacgc catctatccg
     1021 ctggtgcagc tctgtgagca gttgctgtcg gacgatcctc tgtttttcct gatcaactcc
     1081 tacaccactg gtcttcagcc ggcagtgctc agctatatga tgaataccgt cttaaaaaaa
     1141 cacggtggtc atatcgccgc agatgagatc ggtctgccgg tttccagcaa cggtctggtg
     1201 cttccgtgcg gggcaagcgg cagatttgag cgctaagggc gggaacgatt ttgtcctgtt
     1261 tttgcacaat ttttgtaaat agttgttttc ctgcatacac aatacaattt ttgtggtacg
     1321 ctgtttttat gcaaaggtga tacggggatc cggatcaccg gcaaataaaa acgaacagga
     1381 gaatgatttc tatggcacag tttgtaaaat tagttcccga gaatttaaag gcgctgcgcg
     1441 aagtaaatcc gcgcctgatg tcttataatg tagaattcgc cgaggtaacc ggcggcacct
     1501 tctggaaagc gtacacaccg gagcaggttg caggaaccga ggaattccat gtcgctccga
     1561 gcgcagacgg cattgctgca atgtacaagg atctgatgca ggtatacgca ccgatcgatc
     1621 tgtacaacga gaagctccgc tccctcgcaa aggagctcgg aaccgcatgg gtgcgtgtgt
     1681 ccggcacctg ggcaaccaag acttactatg attttgacaa tgcaacaggc ggtattgctc
     1741 cggagggcta cttaaatgtc ctgacaaagg agcagtggat tggtgtgctc gacttcgtaa
     1801 aagcgatcga tgcgaaactg atgatttccg ttgcgaactg cccgggtctg cattccgcag
     1861 aggaaccgtg gaatccgagt gaggcggaga agatttttcg tctgagcaaa gaacacggcg
     1921 taccgatcga cggcgccgag ttcgcaaacg aaccgaacat gatggaggat accggcttcc
     1981 cggctggtta tacaccggct gattaccacc gcgatcagga tctgtttttc gcgtgggtac
     2041 gcgccaacta tccggaatgt atctgtatcg gaccgagcag tgttggcgag ggtcttacca
     2101 tcaatggcgg tgatgacaac agcaagagtg gtggtatcga gcagcttgta agagagaact
     2161 gctccacaac cgacctgtta gaggacacca aagagccgtt ggacgtattc agctaccatt
     2221 attacaacgg tgtatccgag cgtcttgctt ccgtgatgcc aagcggacac tggctggcag
     2281 ataccgccca taccgaggcg tatctggacg ttgcattaaa ctgtgcgcgc acctatgctc
     2341 cactccgcga caaatactgc ccgggcggcg agatgtgggt aacggagtcc ggcgacgcag
     2401 gcggtggcgg tgatacatgg gcttccacgt atctggatgt tttccgtacc ttaaacgagc
     2461 tcgctggatt cgcagcgatc acagacggcg ttattttcca caatacactg gcttcttccg
     2521 actacggctt cctggcccgc gaagtatttg atccgcgccc gaactatttt gccgtattgt
     2581 tgtggaaccg cctgatgggt acgaccgttt acgataccgc agagccaatc cgcgagggcg
     2641 cacatgtctt cgcacattcc cgcaaagatg gaaaagaggg cgttgtatat cttgtgatca
     2701 acaactcgct gacagaggca accaccgtag agcttccgaa gagcgcagag cgctacacac
     2761 tcgccggcga gggcggcaac gtgcgtgccg ctgtcatgac tttaaatggc aagccacttg
     2821 ttctgggtgc cggcaattcg cttccggaac tcactccggc agcacaggaa gccggaacag
     2881 tgactcttgc accggcaacc tgtacattct tcgtactgta agcagcgcgg ggaagcaatc
     2941 tgcaccgatg agtaagccgt ataagctggc tagtaagttg cattactggc tacagtgggg
     3001 cttaagtaag ttgcaacggc atgtgcgtta atacttgtat cggctggctg gcaatatacc
     3061 ggtatgtaat ctgtaatgac tggctacatg gataggcacg ttgcatacac tggctgggct
     3121 gcaacgttgt gtgacctgca acgactgggt aaatttgtaa gttacatcga ctggcaagct
     3181 gcagggctga gccttacatg cacccacagg agctttcttg gtcaatcatt gtttgctgat
     3241 gttgtgcata agatcaaaac gggtctacag aggacttttt ctctctgtag acctgttttt
     3301 gcgtacaatc ttctattttt ttgacttaca tggctgcatt ttggcttccg ccgggtctgg
     3361 caacaccttt ttcctctccc agacccgaaa ctaatctctt ttcgggtctc acgggctggt
     3421 ttttcattcc cagacccgcg aacagccctt ttttcttccc acgcgcataa taacaaggtc
     3481 ccggcgggtc tggagagacg ttttttctct ctcagacccg ccggaacctt gcacaagacc
     3541 atttttcttc ttaatatccg gtcggacggt cttttttccc gaccttgagg cgcgacatcg
     3601 cgcgcgccag cgatgctttg gaacgataat actcctgaat gctctgtttc tggcggagct
     3661 gctcctgtgc acgctctttt gcctcctcgg cacgccgcac atcgatctcc tccggggact
     3721 cacaggtgtc aacaatcacc attgcacggt tattgatgat ctgtgcaaat ccggtgcctg
     3781 tcacacccac gatctcctcg cctgtttcgt ccgtgatgcg gatctcgccg atctcaacgg
     3841 caaacaccat attttcgtga tgcgcctgga ttgctttctc tccgtcatgc agcggcagaa
     3901 cgacctgctc acatcttccg tcaaaaaaga cacggtcaca ggcaatcact ttcagactaa
     3961 atgtattcat gcccccatct ccttcgcacg ctcgatcaca tcatcgatcg tacctacatt
     4021 gaagaacgct gcttccggat actcatccat ctcaccgtca ataattgcct tgaatcctct
     4081 taccgtctcc ttaagcggca catacttgcc cggcacgccg gtaaagttct ctgccacgtg
     4141 gaacggctgg gataagaact tctggatctt tcttgcacgg tataccacga gcttatcctc
     4201 ctcggacagc tcctccatac cgagaattgc aatgatatcc tgcagttctg tgtacttctg
     4261 taaaatctcc tgtactcttc tggcggtctc ataatgctct tttccaacga cgtcctcctc
     4321 aaggatacgg gagttggact caagcggatc caccgccgga taaataccct gctctacgat
     4381 ctttctggaa agtaccgtgg tggcatccag atgtgcaaat gtcgtcgccg gagccgggtc
     4441 tgtcaggtca tctgccggca cgtaaacagc ctgtaccgaa gtaacagatc cgttctttgt
     4501 ggaagcaatc cgctcctgca gttcgcccat ctccgtcgcc agggtcggct gataacctac
     4561 tgcggacggc atacgtccaa gcagcgcgga tacctcagat cctgcctgtg taaaacggaa
     4621 aatattatcg atgaacagaa gtacattctg atgctcctca tcgcggaaat attctgccat
     4681 cgtaagtccc gtctcagcaa ctctcatacg tgctcccggc ggctcgttca tctgtccgaa
     4741 cactaacgcc gt
//
LOCUS       DBMDQJ010000094         4710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6949_length_4710_cov_1.332827, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000094 DBMDQJ010000000
VERSION     DBMDQJ010000094.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6949_length_4710_cov_1.332827"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="ACZO9R_02915"
     CDS             <1..591
                     /locus_tag="ACZO9R_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912059.1"
                     /translation="SAMQQATVQLGKIGIQAIVNTMGISVMAAFAVVNRIDDFAYTPE
                     QNIAHAMTALLAQNKGAGKKERIREGFRCGLWIEMIYALFICVVCFVFARPLMKLFVT
                     DEEVISLGVIYLHAIAVMYFLPAATNGIQGYFRGMGDLKVTLSSSFINMGVRVAAAVP
                     LVFSMKLGIVALPVAYLIGWLAMLAAETPLLFRRKE"
     gene            693..1598
                     /locus_tag="ACZO9R_02920"
     CDS             693..1598
                     /locus_tag="ACZO9R_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912060.1"
                     /translation="MANLFQLLLAQIHPRQGRPAGEPERAGGYKTHPKRESNNKVRET
                     RVLETGESTQERERIQTEVEKEDFLLSQIDEFREKAKQLQDLLAAKENKVQELQSIVN
                     EREGKAEEWQHILVERQEEADKIVTEFTKKVDDLADRVTAKMTEIETSISGQVAEVKR
                     ASEEQLASNRKLNEDQIAANKKLSEEQLATNKQFLEEQAIANKKLSEGQIAEVKALLE
                     NATAQLESIKTDLSEKVHSENVKCYRNIQDLFNEFDSKIEKMDEMEQGVGSVKGYVKC
                     LSWFAILNFVVLIGFILYSLGVFNF"
     gene            1620..3277
                     /locus_tag="ACZO9R_02925"
                     /pseudo
     CDS             1620..3277
                     /locus_tag="ACZO9R_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
     gene            complement(3315..4157)
                     /locus_tag="ACZO9R_02930"
     CDS             complement(3315..4157)
                     /locus_tag="ACZO9R_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR0912061.1"
                     /translation="MPEYKKVGYLTTSFKMFHLKDTSRREFHYHYHDFHKILILLGGD
                     VTYCIEGRTYQLAPGDIVLVNAGEVHKPEIHSDLPYERIILYVSPDFLTEYAGEDCDL
                     SFCFKQAYREQAHILRLQDSKGGRLGASIRALDVSLNDDDYAAALHHRLLFLEFMIQL
                     NRAALHHHIEFVGDSASNEKILSILTYLNAHLTEDLSIDDLASRFYLSRSYLMHTFKE
                     QTGYTIGGYLLTKRLFLAKELIAAGTPITEVCYSCGFQNYSTFSRAYKKSFGESPRDY
                     RQSL"
     gene            4307..>4710
                     /locus_tag="ACZO9R_02935"
     CDS             4307..>4710
                     /locus_tag="ACZO9R_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR0912062.1"
                     /translation="MKNFSNYTDFDTKENLHFESKAVHGALGTEPLTGAVSMPIFQAA
                     TFRHPELGESTGFAYSRLENPTRQELERTMAILEGGIKGFAFSSGQAANMAVFSLLNP
                     GEHVILSDDIYGGTFRIIGDVFGKYGIEHTYV"
BASE COUNT         1229 a         1021 c         1409 g         1051 t
ORIGIN      
        1 agcgccatgc agcaggcgac ggtacagctc ggcaagatcg gaatccaggc gattgtcaat
       61 acaatgggaa tctctgtcat ggcggcgttt gcagtcgtaa accgcattga tgattttgcc
      121 tacactccgg agcagaatat cgcccatgcg atgaccgcgc tgctggcaca gaacaaaggt
      181 gccggcaaaa aggagcggat ccgggaggga ttccgttgcg ggctgtggat cgagatgatc
      241 tatgcgctct ttatctgtgt ggtctgcttt gtgtttgcgc gtccgctgat gaagctgttt
      301 gtgacggatg aggaggtgat ctccctggga gtgatctatc tgcatgcgat tgcggtgatg
      361 tattttctgc cggcggccac aaacgggatt cagggatatt ttcgcggaat gggcgacctc
      421 aaggtgacgc tcagcagcag ttttatcaac atgggagtgc gggtggcggc agccgttccg
      481 ctggtatttt ccatgaagct tggaattgtg gcactgccgg ttgcctacct gatcggatgg
      541 ctggcaatgc tggcggcgga gacaccactg cttttccgcc ggaaagagtg agcgggaggc
      601 ggttgctttt tcctgcttta caaagaaaaa tgataatgat agaataccca taggtgtttt
      661 ttgttcggaa gacacaaagg gaggaagcgt ttatggcaaa cctgtttcag cttttactgg
      721 cgcagattca ccccagacaa ggaagacctg caggagagcc ggaacgggcg gggggatata
      781 agacacaccc gaagcgcgag agcaataata aggttcggga gaccagggtt ttagagaccg
      841 gggagagcac gcaggaacgg gaaaggatac agacagaagt ggaaaaggaa gatttcttat
      901 taagtcagat cgacgagttt cgtgagaagg cgaaacagct gcaggatctg cttgcagcga
      961 aggagaataa agtacaggaa ctgcagtcga ttgtcaacga gcgggaaggc aaggccgagg
     1021 agtggcagca cattctggtg gagcgccagg aggaagctga caagatcgtg acagagttca
     1081 cgaagaaggt cgatgatctg gcagaccgtg tgacagcgaa gatgaccgag attgagacaa
     1141 gcatctcggg gcaggttgcg gaggtaaagc gtgcgagcga ggagcagctt gcatcgaacc
     1201 gcaagctgaa tgaggatcag attgctgcaa ataaaaagct ttccgaggag cagcttgcga
     1261 caaacaagca gtttttggag gagcaggcaa tcgcgaacaa gaagctttcc gagggacaga
     1321 tcgccgaggt caaggcgctg cttgagaatg cgacagcaca gctggaatcc atcaagacag
     1381 atctctccga gaaggtacat tcggagaacg tgaagtgcta ccgcaacatt caggatctgt
     1441 tcaacgaatt tgattccaag atcgagaaga tggacgagat ggagcaggga gtcggatcgg
     1501 tgaagggata tgtcaaatgt ctttcctggt ttgcaattct gaactttgtt gttctgatcg
     1561 gattcatcct gtattctctc ggtgtgttta atttttagct ttaaggaata gaagatcata
     1621 tgagtacaga atcaaagaaa gtgtgggtcg tggggcataa gaatccggat acggattcga
     1681 tatgcgccgc gatttcttat gcatatctga aaaaccagag cggggacaag aagacttatg
     1741 tggcaaagcg tgcgggcgcg gtgaatgagg agacccgcta cgtgttagag cgctttggcg
     1801 tggaggaacc gccgcttgtg agctatgccg gtgcacagat caaggatatc aatatccgca
     1861 agacggccgg tgtcagcaat cagatttcgc tgaaacgggc atgggagctg atgaaaaagc
     1921 tggaggttgt aacgcttccg gtcaccaatc agttcggcaa gctggaaggt gtgatcgtca
     1981 ccaaggatat tgcgacgtcc tacatggacg tgctggacaa ctgcgtgctc tcaaaagcgc
     2041 gcacacagta caagacgatt gcggagacga tcgacggtga ggtctacgcc gggaatgagc
     2101 atgcacattt tgtgcgcgga aaagttgtga ttgcaacctc caacccggaa tatatggcgg
     2161 attatatcga ggacgacgat ctggtgatct taggcgaccg cgaggaggcg cagatgcagg
     2221 cgatccggtc gaacgcgagc tgcatcatca tcggcggcgg tctcgaggtg gcggaagaag
     2281 tcaaaaagct tgcggagaag cgggactgcg tgattatcac aacgccgttc gacacatttt
     2341 ccgtggcgcg tctgatcaat cagagtatgc cgatcaagca gtacatgacc agacgggagc
     2401 tcgtcacgtt tgacattgac gattatgtgg acgacgtcaa ggatgtcatg tcgcgggtgc
     2461 gccacagaga tttcccggtg ctcggctcaa acggcaacta cgtcggaatg atttccagac
     2521 gtaacctcat gaatatgcag aaaaagcaga tcattcttgt cgaccacaac gagaagtcac
     2581 aggcggtgga cggaatcgga gaggcggaga tcttagagat catcgaccat cacagactgg
     2641 gaagcttaga gaccgtatcg ccggtttatt tccggaatca gccgctcggc tgtacctcga
     2701 caattatcta tcagatgtat caggagcaga gggtggagat tccgaaagag atcgcgggac
     2761 ttttgctctc ggcgatcatt tccgacacgc tgatgttccg ctcgccaacc tgtacaccgt
     2821 ttgacaaggg cgttgcaaag cggctcgcag agattgcgga cgtggatatt gaggatcatg
     2881 caaagaaaat gttccgcgcc ggaagtgatt tcaagaacaa gacgaccgaa gagattttct
     2941 atcaggattt caagattttc cacacggaag taatgtgttc gctgttgtcc agatcagcgc
     3001 catgagcgga gaggaattag aacagatcgg agaacagctc cgcccgttcc tgccgcaggt
     3061 gctcggcgag aagcgtttaa atatggtata tgtcatgctg acggatattt tggaggaatc
     3121 ttccaagatt atatttgcgg gggaggatgc cggaaaaatt ctggcgcatg cgttcaagaa
     3181 gcaggaggat gcggatggca tcctgttgga cggcattatc tcacgtaaga aacagatgat
     3241 acctacactc atgaatgaga tgtcagagcg agcgtagctc gctctgtgag catagcccgc
     3301 tctgcgggca tagcttaaag gctttggcgg taatccctcg gggattcccc aaagcttttt
     3361 ttgtatgctc tcgagaaggt cgagtaattc tgaaagccgc aggaatagca tacttccgtg
     3421 atcggcgtgc cggcggcgat cagctctttg gcgagaaaca gccgcttagt gagcagatag
     3481 ccgccgatgg tgtagccggt ctgctccttg aacgtgtgca tcaggtagga gcggcttaag
     3541 taaaagcggg aggccaggtc atcgatcgag agatcctcgg tcagatgagc gtttagatag
     3601 gtgagaatcg agagaatctt ctcgttggaa gcggagtctc ccacgaattc aatgtggtgg
     3661 tgcagggcgg cgcggttgag ctgaatcata aattccagga ataaaagccg gtggtgcagc
     3721 gccgccgcat agtcgtcgtc gtttaaggag acgtccagcg cgcggatgga ggcgccgagc
     3781 ctgccgccct tggaatcctg cagacggagg atgtgtgcct gctcgcggta cgcctgcttg
     3841 aagcagaagg agagatcgca gtcttccccg gcgtactccg taagaaaatc cggggagaca
     3901 tataaaatga tgcgctcgta gggaagatcc gagtggatct cgggtttgtg tacttccccg
     3961 gcattgacca ggacaatgtc gcccggggca agctggtagg tgcgcccctc aatacagtag
     4021 gtcacgtcac cgccgagaag aatcaggatc ttgtgaaaat cgtgatagtg gtagtgaaac
     4081 tcccggcggg aagtatcttt taaatgaaac atcttaaagc tggtggttaa gtatccgacc
     4141 tttttgtatt ccggcatcgc gtgcgtcctc ctttttctat tactgtatta taaaaaagca
     4201 ctttttgcaa tataaaaagc actttgagac tatatattat aaaaagtgtt ttgtataata
     4261 gatgtatatg aggaaacgca tgtccaaaca gatggaggga aataacatga aaaatttcag
     4321 taattatacc gattttgata caaaggaaaa tctgcacttt gagtccaagg ccgtgcacgg
     4381 cgcgctggga acggaaccgc tcaccggagc agtcagcatg ccgatcttcc aggcagcgac
     4441 attccgccat ccggagcttg gcgagtcgac cggctttgca tacagccgtc tcgagaatcc
     4501 gaccagacag gaattagagc gcacgatggc aattcttgag ggcggaatca agggattcgc
     4561 attttccagc ggacaggcgg caaacatggc tgtcttttcc ctgttgaatc cgggcgagca
     4621 cgtcatttta tcggatgata tttacggcgg aaccttccgc attattgggg atgtttttgg
     4681 aaaatacggt attgagcata cctatgtaga
//
LOCUS       DBMDQJ010000095         4707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6960_length_4707_cov_1.404472, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000095 DBMDQJ010000000
VERSION     DBMDQJ010000095.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6960_length_4707_cov_1.404472"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="ACZO9R_02940"
     CDS             complement(<1..647)
                     /locus_tag="ACZO9R_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912063.1"
                     /translation="MKKRWMALLGAAVVTINLFAGCGSTGAEQTGNNADGTATEAVQG
                     KDSAGGGNAAQSVAGSADTVIVAMSPSSEPAAGFNPCINWGCGEHCHEPLIQSTLIRT
                     NVDMEFENDLATDYSVSEDGLTWTFHIRDDVKFTDGEPLTASDVAFTFNTALATPNSE
                     ADLSMLDSVEALDDTTVIFHMSKPYNAFLYTLAVFGIVPEHCYDEAAYGENPVGSG"
     gene            complement(764..1930)
                     /locus_tag="ACZO9R_02945"
     CDS             complement(764..1930)
                     /locus_tag="ACZO9R_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP-loop family protein"
                     /protein_id="HIR0912064.1"
                     /translation="MYQRTIEELQKDEREKVIIDIRREADFLRETYPGAKHIYWEEFE
                     AHRDEVPKDRPVYLICYTGERSDELARELLEEGYEVYSVQGGYRAYLRMKLLESMEQT
                     KAPDAGEKNADRCSEIERSIVKKFRKEIWRKFTKAINEYELIQDGDKIAVCISGGKDS
                     MLMAKLFQELERHGKKNFEVVFLVMNPGYNEINYETIVNNAKILNVPITVFTSEIFDI
                     VAGEEESPCYLCARMRRGHLYSKAKELGCNKIALGHHFDDVIETILMGMLYGAQVQTM
                     MPKLHSTNFPGMELIRPLYLVREEDIIHWMNYNDLHFIQCACRFTESCASCGGTEKGS
                     KRAEIKDLIRELRKKNPYIEKNIFRSVENVNLNTVIGYKKDGVRHHFLDEYDKA"
     gene            complement(1947..2702)
                     /locus_tag="ACZO9R_02950"
     CDS             complement(1947..2702)
                     /locus_tag="ACZO9R_02950"
                     /EC_number="6.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_function: GO:0061503 - tRNA threonylcarbamoyladenosine
                     dehydratase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HIR0912065.1"
                     /translation="MLNQFSRTELLLGKEAMERLSRARVAVFGIGGVGGYTVEALVRS
                     GVGAIDLIDDDKVCLTNLNRQIIATRSTVGKYKVEVMRDRILDINPDCKVEVHKCFYL
                     PETKDEFDFSSYSYVVDAVDTVTAKIALVMQSQETGTPIISSMGAGNKLNPAMFEVAD
                     IYKTSVCPLAKVMRRELKKRGVKKLKVVYSREQPVTPIEDMSISCRAHCICPPGAKHK
                     CTERRAIPGSNAFVPSVAGLIIASEVIKDLAAE"
     gene            complement(2763..3686)
                     /gene="nadA"
                     /locus_tag="ACZO9R_02955"
     CDS             complement(2763..3686)
                     /gene="nadA"
                     /locus_tag="ACZO9R_02955"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR0912066.1"
                     /translation="MEAERAKRIKELQKEKDVVILAHYYVDGAVQEIADYVGDSFYLS
                     KVATKVPQKTILFAGVTFMGESAKILNPDKTVVMADECADCPMAHMITADRIAEVREE
                     YEDLAVVCYVNSTAEIKAVSDVCVTSSNAVKVVSNIPQKNIFFVPDNNLGRYVAEKLP
                     EKHFIFHDGFCHVHKSIHAAELQAAKDACPDALVLTHPECTEDVVAMSDFVGSTSEII
                     DFATQSDCKKFIICTEMGVFFELQQKNPDKKFYSVGHRQFCPNMKKVTIDKVEQVLEK
                     LGSGELEEITLSDEILTRAAKPLQRMLELAK"
     gene            complement(4014..>4707)
                     /locus_tag="ACZO9R_02960"
     CDS             complement(4014..>4707)
                     /locus_tag="ACZO9R_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR0912067.1"
                     /translation="LAAVAAAKGYHAILTLPDTMSVERRNLLAAYGAELVLTEGAKGM
                     KGAIAKAEELKESTPGAVILGQFVNPANPAAHKATTGPEIWEQTDGKVDIFVAGVGTG
                     GTVTGVGEYLKEKNPNVKIVAVEPATSPVLSKGTPGAHKIQGIGAGFVPDVLNTKVYD
                     EVITIENDDAFTEGRAFARSEGILVGISSGAALKAAAILAARPENKGKTIVALLPDSG
                     DRYLSTSLFAKN"
BASE COUNT          983 a         1464 c         1038 g         1222 t
ORIGIN      
        1 ccggaaccga ccgggttctc cccgtaggct gcttcatcgt agcagtgctc cggcacgatg
       61 ccgaacaccg ccagtgtata caaaaacgca ttatacggct tgctcatgtg gaaaatcacc
      121 gtggtatcgt caagtgcctc cacggaatcg agcatactta agtccgcctc cgaattcggc
      181 gtggcaagcg ccgtgttaaa cgtaaatgcc acatcagacg ccgtcagcgg ttccccgtcc
      241 gtgaacttca catcgtcccg gatgtgaaac gtccaggtca gtccgtcctc actcacgctg
      301 taatccgtcg ccagatcgtt ctcgaactcc atatccacgt tcgtccggat cagcgtcgac
      361 tggatcagcg gttcatggca gtgttcccca cagccccagt tgatgcacgg gttaaatccc
      421 gccgccggct ccgaggaagg actcatcgcc acgatgaccg tatctgcgct tcctgccacg
      481 ctctgcgccg cattcccgcc tccggcgctg tcttttccct gtactgcctc tgtcgccgta
      541 ccatctgcgt tattgcctgt ctgctccgca cctgtgctgc cgcatccggc aaacagattt
      601 atcgtcacta ccgctgcccc aagcagcgcc atccacctct ttttcattat ttattctccc
      661 ttgttaaaat cgccggcatt tctgcccagt gcacaaagga tagcatgaaa aagaggggcc
      721 gcacaagccc ctcgggggga taaaaatgac acaaaatcaa agattatgct ttgtcatatt
      781 catccagaaa atgatggcgg acaccatctt ttttataacc gatcacagtg ttcaggttga
      841 cgttctccac actgcggaag atattcttct caatgtaagg attcttcttg cgaagctccc
      901 ggatcagatc cttgatctcc gcgcgcttgg aacccttctc cgtaccaccg caggaagcac
      961 agctttccgt gaaacggcag gcacactgaa taaagtgcag gtcattgtaa ttcatccagt
     1021 ggatgatgtc ctcctcgcgc accagataca gcggacggat cagctccatc cccgggaagt
     1081 tcgtactgtg cagcttcggc atcatcgtct gtacctgcgc cccgtaaagc attcccataa
     1141 ggatggtctc gatcacatcg tcaaaatgat gccccagcgc aatcttgttg cagccgagct
     1201 cctttgcctt gctgtagaga tgccctctgc gcatgcgcgc gcacaggtag cacggagatt
     1261 cctcctcacc cgccacaatg tcaaagatct cggaggtaaa taccgtgatc gggacgttta
     1321 agattttggc gttgtttaca atcgtctcgt agttgatctc attgtacccc ggattcatga
     1381 cgaggaatac cacctcgaaa ttcttcttgc cgtggcgctc cagctcctga aacagcttcg
     1441 ccatcaacat ggaatctttt cctccggaaa tacagacggc gatcttgtca ccgtcctgga
     1501 tcagctcgta ttcgttgatc gcctttgtga acttccgcca aatctctttg cggaactttt
     1561 tgacaatgct gcgctcgatc tcgctgcagc ggtccgcatt cttctccccg gcgtccgggg
     1621 cttttgtctg ttccatggat tccagcagct tcatgcgcag gtacgcacgg tatccgccct
     1681 gcacactgta tacctcataa ccctcctcta aaagttcgcg cgccagctcg tcgctgcgct
     1741 ctcccgtata gcagatcagg taaaccgggc ggtccttggg cacctcgtct ctgtgcgcct
     1801 cgaactcctc ccagtagata tgcttcgctc ccggatatgt ctctctcaaa aaatctgctt
     1861 ctctgcggat gtctatgatt actttttccc gctcgtcctt ctgcagttct tctatggttc
     1921 tctgatacat gcctgtccct cattctctat tctgccgcaa ggtccttgat gacctccgat
     1981 gcaatgatca atcctgccac cgatggtaca aatgcattgg aacccggaat cgcacggcgc
     2041 tccgtacatt tatgctttgc tcccggcggg cagatacagt gcgcacggca gctgatcgac
     2101 atatcctcga tcggcgtgac cggctgctcc ctggaataca ccacctttag tttcttcact
     2161 ccgcgctttt tgagttcgcg gcgcatgacc ttcgccagcg ggcagaccga cgtcttatag
     2221 atgtccgcca cctcaaacat tgccggattc agcttgttgc cggcgcccat gctgctgatg
     2281 atcggcgttc ctgtctcctg cgactgcatg accagcgcaa tctttgccgt cacagtatcg
     2341 actgcatcca cgacatacga atagctggaa aaatcgaact cgtcctttgt ctccggcaga
     2401 tagaagcact tgtgcacctc caccttgcag tccgggttga tgtcgagaat gcggtcacgc
     2461 atgacctcca ccttatattt ccctacggtg ctgcgcgttg cgatgatctg acggttcaga
     2521 ttggtaaggc agaccttatc atcgtcgatc agatcgatgg ctcccacacc gcttcgcacc
     2581 agcgcctcca cggtgtagcc tcccacaccg ccaatgccaa aaacagcgac cctcgcacgc
     2641 gagagccgct ccatagcctc ttttcctaat aataattctg ttctggaaaa ttgatttaac
     2701 atagatactc ctatatattg taacgtttac gcctgtccgc ttctatcata tagcgtccgc
     2761 cgctattttg caagttccaa catgcgttgc agcggctttg cggcacgggt caggatctcg
     2821 tcagagagcg tgatctcctc gagttctccg ctgccgagct tctcaagcac ctgctcgact
     2881 ttatcaatcg tcactttctt catgttcggg caaaactgtc tgtgtcccac cgaatagaac
     2941 ttcttatccg gattcttctg ctgcagttca aagaatacgc ccatctccgt gcagatgatg
     3001 aactttttgc agtcgctctg cgtcgcaaaa tcaataatct cagacgtgct tcccacgaaa
     3061 tcggacatcg caaccacgtc ctcagtacac tccgggtgcg tcagcaccag tgcgtccgga
     3121 caggcatcct ttgccgcctg cagctctgcc gcgtgaatgc tcttgtgcac atggcagaag
     3181 ccatcgtgga aaatgaaatg cttctccggc agcttctctg ccacatatct gccgaggttg
     3241 ttgtccggca cgaagaaaat attcttctgc ggaatgttgg acacgacttt taccgcgttg
     3301 gaggaagtca cgcacacatc cgataccgcc ttaatctccg ccgtggagtt cacatagcag
     3361 accactgcca gatcttcgta ttcctctctc acttccgcga tccggtctgc cgtaatcata
     3421 tgtgccatcg ggcagtccgc acactcgtcc gccatcacaa ccgtcttatc cgggtttaag
     3481 atcttcgcgc tctcacccat aaacgtcact ccggcaaaca gaatcgtctt ctgcggcacc
     3541 ttcgtcgcaa ccttgctcag ataaaaggaa tcacccacat aatcggcaat ctcctgcacg
     3601 gcgccgtcca cataataatg cgcaagaatg acaacatcct tctctttctg caattccttg
     3661 atccgcttcg ctctctctgc ttccattttt atctccgttc tctatacatc cgcttttcag
     3721 gtgacaagac acctgtcacg atagattgtc attgtgatac tttgatatca tatcatgttt
     3781 tcagcaaatg tccagagttt tcctgtctct gcgcctgttt ttccgggatg ccccgcactt
     3841 ttcccatgca ctaacagacc ttccgcactt tccacggtca aaaacagacc ttccacacat
     3901 ttcacggtca aaaacagacc ttccacacat ttcacggtca aaaacagatc ctccggcggc
     3961 acaggcaaaa ccgtgctgcc aaaggatccg gcatttgctc ttcgagcggt ctcctagttc
     4021 tttgcaaaca gggaggtgga cagatatctg tctccggaat ccggaagcaa agccacaatc
     4081 gtcttgccct tgttctccgg tcttgcagcc agaatggctg ccgcctttaa tgctgcgccg
     4141 gaagagatac ctacgaggat accttcgctg cgtgcaaacg ctcttccctc tgtaaatgcg
     4201 tcgtcgttct caatcgtgat gacttcatcg tacacctttg tgttgagtac atccggaaca
     4261 aatcctgcgc caataccctg aatcttgtgc gcacccggtg tgcccttaga taataccggg
     4321 ctggttgccg gctctaccgc aacgatcttg acgttcggat tcttctcctt taagtactcg
     4381 ccgacaccgg ttaccgttcc gccggtacct acgcctgcta caaagatatc taccttaccg
     4441 tctgtctgct cccagatctc cggtcctgtg gttgccttgt gtgccgccgg atttgccggg
     4501 ttcacgaact gtcctaagat cacagcgccc ggtgttgact cctttaattc ctctgctttg
     4561 gcgatagcgc ccttcatacc cttcgcgccc tcagtcagca ccagctcggc gccgtatgct
     4621 gccagaaggt ttctgcgctc tacgctcatc gtatccggca gtgtcaggat ggcatggtag
     4681 ccctttgccg cagcaactgc tgccagt
//
LOCUS       DBMDQJ010000096         4694 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_6989_length_4694_cov_1.217287, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000096 DBMDQJ010000000
VERSION     DBMDQJ010000096.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4694)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4694)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4694
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6989_length_4694_cov_1.217287"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="ACZO9R_02965"
     CDS             <1..498
                     /locus_tag="ACZO9R_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009264893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HIR0912068.1"
                     /translation="APSDMMDGRVAAIREALDENDCKNTMLMAYSAKFASGFYGPFRE
                     AAHSVPSFGDRRTYQMDPANRREAIRECLSDIEEGADIIMVKPALAYLDVVREVMDRT
                     DYPLAAYNVSGEYAMVKAAAANGWIDERRIVMEIMTGIKRAGAKIIITYHALDVAKWL
                     DEERK"
     gene            538..1809
                     /gene="hemL"
                     /locus_tag="ACZO9R_02970"
     CDS             538..1809
                     /gene="hemL"
                     /locus_tag="ACZO9R_02970"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009251701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HIR0912069.1"
                     /translation="MQYTESERLFEASKKCILGGVNSPVRAYQAVHRTPVFVERASGS
                     HIYDVDGNAYIDYVCSWGPCILGHAYLRVIKAVQAACENGLTYGAPTGKELKLAELIR
                     SCIPSMEVSRLVSSGTEATMSAIRVARGFTGRDKVVKFRGCYHGHSDGLLVKAGSGAL
                     TQSVPDSKGVPADYTKNTLVAEYNQPDSVQKLFETQGKEIAAIIVEPVAANMGVVLPE
                     PGFLEFLRKITEDYGALLIFDEVITGFRLGIGGAQGYFHVTPDLTTLGKIIGGGMPVG
                     AYGGRKEIMDMVAPVGPVYQAGTLSGNPIATTAGIETLTILKETPEIYTQIEASARTL
                     AEGMRGHFGESVCVNQIGSLMSIFFTAQKVKDYETATSSDISRYADYFNYMLDHQIYL
                     APSQFEAMFVSAAHTAEDIAQTCEVLRGYGR"
     gene            2048..3232
                     /gene="nifS"
                     /locus_tag="ACZO9R_02975"
     CDS             2048..3232
                     /gene="nifS"
                     /locus_tag="ACZO9R_02975"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HIR0912070.1"
                     /translation="MEKKLIYMDNAATTPVKPEVLDAMLPYFTEKFGNPSSIYSISSE
                     NKKAITDAREVIAKTINTTPENIYFTAGGSESDNWALKATADAYASKGKHIITTKIEH
                     HAILHTCEYLETKGFEITYLDVDENGLVKLDELTAAIRPDTILISVMFANNEIGTIEP
                     IAEIGKIAHEHGVLFHTDAVQAYTQVPIDVEAMNIDMMSTSGHKINGPKGIGFLYIRK
                     GVKIKSFIHGGAQERHRRAGTENVTGIIGLAKAAEIATANMKERTAEEIKVRDHLIER
                     IEKEIPYAKLNGDRVKRLPNNVNFSFQFVEGESMLILLDSKGICASSGSACTSGSLDP
                     SHVLLAIGLPHEIAHGSLRLTISDQITMEDADYVVDNLKEIVNHLREMSPLYEDFIKK
                     NK"
     gene            3259..3696
                     /gene="nifU"
                     /locus_tag="ACZO9R_02980"
     CDS             3259..3696
                     /gene="nifU"
                     /locus_tag="ACZO9R_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HIR0912071.1"
                     /translation="MYSEKVMDHFQHPRNVGEIEDASGVGTVGNAKCGDIMRMYLDID
                     ENQVIRDCKFKTFGCGAAVATSSMATELVKGKTIYEALQITNKAVMEALDGLPPVKVH
                     CSLLAEEAIHAALWDYAEKHNIKIEGLEKPKNDISEGLEEEDY"
     gene            3699..4283
                     /locus_tag="ACZO9R_02985"
     CDS             3699..4283
                     /locus_tag="ACZO9R_02985"
                     /inference="COORDINATES: protein motif:HMM:NF024395.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3877 family protein"
                     /protein_id="HIR0912072.1"
                     /translation="MNRQDEADYTALKEYLCDMILEEQLKLGYERETIRFYSPSSSIG
                     HVLKLSDRSKPAVLAALEGFGAYAADTLGEVAVSGASGGRICFLIPAAGAEYVHAHGV
                     AHPFLKDLIDCFGRHDVTREQVWELFRHYSKSARIIEIKSDEFDDVCYFEDGNPDSYL
                     YCVKFDEGHAFYHRFLKEDFEELFGEASRMKGAR"
     gene            4285..>4694
                     /locus_tag="ACZO9R_02990"
     CDS             4285..>4694
                     /locus_tag="ACZO9R_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002590232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR0912073.1"
                     /translation="MKITTKGRYAIHILYDLAVNGTEHPVSVKEMATRQEISVKYTEQ
                     IISILNKAGYVSSSRGFQGGYRLTKPLDEYVIGDILRLTEGSLAPVECLANAENPCPK
                     QQKCATIMLWEKVDKAIRDVVDHVTLQDLLDWQK"
BASE COUNT         1278 a         1017 c         1382 g         1017 t
ORIGIN      
        1 gctccatccg atatgatgga cggaagagtg gcggcgatcc gtgaggctct ggatgagaac
       61 gactgcaaaa ataccatgct gatggcgtac agcgcaaagt ttgcatccgg tttttacgga
      121 ccgttccgcg aggcggcaca ttccgtgccg agctttggcg accgcagaac ttaccagatg
      181 gatccggcaa accgcaggga agccatccgg gaatgtctgt ccgatattga ggagggcgca
      241 gacatcatta tggtaaagcc tgcgcttgcg tatctggatg tcgtaagaga ggtcatggac
      301 cgtacggatt acccgctggc ggcgtacaat gtgagcgggg agtatgcgat ggtaaaggcg
      361 gcagcggcaa acggctggat tgatgagcgg cggatcgtga tggagatcat gaccggcatc
      421 aaacgcgcgg gtgcgaaaat catcatcacc tatcacgcgc tggatgtggc aaagtggctg
      481 gacgaggaga gaaagtagtg gaagagaatg gcagcagtgc cggaaatgag gagaaatatg
      541 cagtatacag aatcagaacg attatttgag gcatcaaaaa aatgtattct gggcggcgtc
      601 aacagtccgg tgcgcgccta tcaggcggtt caccggacgc cggtctttgt ggagcgggcg
      661 agcggttccc acatctatga tgtggacggc aacgcgtata ttgactatgt gtgttcttgg
      721 ggaccgtgca ttctgggaca tgcctatctg cgcgtaataa aagccgtgca ggcggcgtgc
      781 gagaacgggc tgacatacgg ggcgccgaca ggaaaggaac taaagcttgc agagctgatc
      841 cgttcctgta tcccgtcgat ggaagtgtcc agactggtaa gttccggaac ggaggcgacg
      901 atgagcgcca tccgtgtggc gagaggattt acgggacggg ataaggtcgt gaagttcaga
      961 ggatgctatc acggacattc ggacggactt ctggtaaaag ccggatcggg agcgctcaca
     1021 cagtctgtac cggacagcaa gggcgtgccg gcggattata cgaaaaatac gctggtggcg
     1081 gagtacaacc agccggattc ggtgcagaag ctctttgaga cgcagggaaa agagatcgct
     1141 gcgatcatcg tggagccggt ggctgccaat atgggagtcg ttttgccgga gccgggattt
     1201 ctggaatttc tgcgaaaaat cacagaagat tacggggcac tgctgatttt tgatgaggta
     1261 atcaccggtt ttcgtcttgg aatcggcggg gcgcagggat attttcatgt gacgccggat
     1321 ctgacgacgc ttggcaagat catcggcggc gggatgccgg tcggcgcata cggcggcaga
     1381 aaagagatca tggatatggt tgcgccggtc ggaccggtat accaggcggg aacgctttcc
     1441 ggcaacccga tcgcaacgac ggcaggcata gagacgctta ccatcttaaa agagacgccg
     1501 gaaatctaca cacagatcga agcaagtgcg aggacgcttg cggagggcat gcgaggacat
     1561 tttggcgaaa gcgtctgcgt gaatcagatc ggatcgctga tgagcatttt ctttactgcc
     1621 cagaaagtaa aggattatga gactgcaacg tcctccgata tttccagata tgcggattac
     1681 tttaattata tgctggatca tcagatatat ctggcaccgt cgcagttcga ggcaatgttt
     1741 gtctctgcgg cacatacggc ggaggatatc gcacagacct gtgaagtcct acggggatac
     1801 ggaaggtaat ttaaggaatc gtatgcggtg aagagaaaac tggatttttt cggagaaact
     1861 gcaggaaaga tgcataaaga gaggatgtta caccgtcggg gcggctgtgc aacgtcctct
     1921 ttttgtatat aatgaagtac aatatctccc gcaggtattt tgcagttgac aaataaaaaa
     1981 aatgatacta gtataaatca tatcatatgg gtatgatatg aaataaaaaa ggaaatggta
     2041 cgagaatatg gaaaagaaac ttatttacat ggataacgca gctaccacac cggtcaaacc
     2101 ggaagtgctt gatgcaatgc ttccgtactt tacagagaag ttcggtaatc catccagcat
     2161 atacagcatt tcttctgaga ataagaaagc aatcacggat gccagagagg tcattgcgaa
     2221 gaccatcaac accacgccgg agaatatcta ttttaccgca ggtggttccg agtctgacaa
     2281 ctgggcatta aaggcaacgg cagatgccta tgcgtccaaa ggaaagcaca tcatcacgac
     2341 gaagatcgag catcatgcga ttcttcacac ctgtgagtat ctggagacga aaggcttcga
     2401 gatcacttac ctggatgtgg atgagaacgg tctggtaaag ctggacgagc ttaccgcagc
     2461 catccgtccg gacaccatct taatctccgt gatgttcgcg aacaacgaga tcggaaccat
     2521 cgagccgatt gctgagatcg gtaagatcgc acatgagcat ggcgtactgt tccacacaga
     2581 tgcagtacag gcatacacac aggtgccgat cgatgtcgag gcaatgaata tcgatatgat
     2641 gagcaccagc ggtcacaaga tcaacggacc gaagggaatc ggtttcttat atatcagaaa
     2701 aggtgtgaag atcaaatcct ttatccacgg cggagcacag gagcgccaca gaagagcagg
     2761 taccgagaac gtaaccggta tcatcggtct tgcaaaggca gcagagatcg ctacggccaa
     2821 catgaaggag cgcactgcgg aggagatcaa agtcagagat catttgatcg agcgcatcga
     2881 gaaggagatt ccgtatgcaa agttaaacgg agaccgtgtg aagcgtctgc cgaacaacgt
     2941 gaacttcagc ttccagttcg tagagggaga gtccatgctg attcttctgg attccaaggg
     3001 aatctgcgca tcaagcggtt ctgcatgtac atccggttcc ctggatccgt cccatgtact
     3061 tctggcaatc ggactgccgc atgagattgc acacggttct ctgcgtctga ccatcagcga
     3121 ccagatcacg atggaggatg cggactacgt cgttgacaac ttaaaagaga ttgtgaacca
     3181 cttaagagag atgtctccgc tgtacgagga tttcatcaag aaaaacaaat aatataaaaa
     3241 agaaagcagg tatatgttat gtatagtgaa aaggttatgg atcattttca gcacccgaga
     3301 aatgtaggag agattgagga cgcaagcggt gtcggcaccg tcggaaatgc aaagtgcggc
     3361 gatatcatgc gtatgtatct cgatattgat gagaaccagg ttatccgtga ctgcaagttc
     3421 aagacattcg gctgcggcgc tgctgtggca accagcagta tggcaacgga gcttgtaaag
     3481 ggcaagacca tttacgaggc gctgcagatc acgaacaagg cagttatgga ggcgcttgac
     3541 ggacttcctc ctgtaaaggt acactgttct cttctcgcag aggaggcaat tcatgcggca
     3601 ctctgggatt acgcagagaa gcataacatc aagatcgaag gtcttgagaa gccgaagaac
     3661 gatatcagcg aaggtctgga agaggaagat tactaagaat gaatcggcag gatgaagcgg
     3721 actatacggc attaaaagaa tatttatgcg acatgatctt agaggaacag ctaaagctcg
     3781 gctatgagcg tgagacgatc cggttttatt caccatcgtc gtcaatcgga catgtgttaa
     3841 agctgtctga ccgctccaag ccggcggttc ttgcggcgct ggagggattt ggcgcatatg
     3901 cggcggatac cctcggtgaa gtggcagtat ctggggcgag cggcgggaga atctgttttc
     3961 tcattcctgc agcgggtgcg gagtatgtgc atgcgcacgg cgtggcgcat ccgttcttaa
     4021 aggatctgat tgactgcttc ggcagacatg atgtgacgag agagcaggtg tgggagctgt
     4081 tccggcacta ttctaagagt gctcgtatca ttgagatcaa aagcgatgaa tttgatgacg
     4141 tgtgctactt tgaggatggc aatccggatt cttatctgta ctgcgtcaag ttcgacgagg
     4201 gacatgcgtt ttatcaccgc ttcttaaaag aggattttga agagctgttc ggtgaagcca
     4261 gcaggatgaa aggggcccga tagcatgaaa attacgacaa agggacgcta tgcgatccac
     4321 atactgtacg atctggcggt caacggcacg gagcatccgg tgtcggtcaa ggagatggca
     4381 acaagacagg agatttccgt caaatataca gagcagatca tttccattct gaacaaggcg
     4441 ggctatgttt ccagcagccg gggatttcag ggcggctacc gtctgacgaa gccgttggac
     4501 gaatatgtga tcggtgatat tctgcgtctg acggagggat cgctcgcgcc ggtcgagtgt
     4561 cttgcgaacg ccgagaatcc ctgccccaaa cagcagaaat gcgcgacgat catgctctgg
     4621 gaaaaagtcg ataaggcgat ccgggacgtg gtcgatcatg tgacattgca ggatcttctg
     4681 gactggcaga agca
//
LOCUS       DBMDQJ010000097         4644 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7069_length_4644_cov_1.550737, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000097 DBMDQJ010000000
VERSION     DBMDQJ010000097.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7069_length_4644_cov_1.550737"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="ACZO9R_02995"
     CDS             <1..280
                     /locus_tag="ACZO9R_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR0912074.1"
                     /translation="KMVSYHLTKGEQLEVILNSTFKQLEDITDVHEMTNAQLKRLINK
                     IEVDKDGNVDIYLRLIGDLGLDETVLIEGDKNREEKDNKTALNCHNHT"
     gene            267..419
                     /locus_tag="ACZO9R_03000"
                     /pseudo
     CDS             267..419
                     /locus_tag="ACZO9R_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611129.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
     gene            503..2419
                     /locus_tag="ACZO9R_03005"
     CDS             503..2419
                     /locus_tag="ACZO9R_03005"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR0912075.1"
                     /translation="MKKIALIMDGWKRFFTYAWPAGVLERIRETNEDVNLYIFNSSGD
                     WSRDEDYNIGEYNIYRLPDLNDFDGIIVDLNNIRYSEVREYVISSAKATGKPVISIAN
                     EIADFYYVGIDNYSAMQEIAAHLYEVHNCRRFWYVMGPDDNYESNRRVAALRDFSETH
                     GILWDARDLYCESYEYKCGVHGFEKLLTLHGKLPDAIICANDNIAVGVCETAEAHGYK
                     APDDFLVTGFDNFDKASYYSPHITTVGHIREQVGYRCADILLRLWRGETVPRFNYTGH
                     QCIFWESCGCDAGIAVDQAEHSRAQIVYGIETDEFEEQVLSLEYELLQCETVREMSRW
                     IPKCIPAMRCDAMYLIMDEHMNDFRELSDYYDRHLIEDEEFCVHGYPEKMQMEFAYED
                     GVVKESEETVVEGIFPTFDYAEGGKDFLFLPLHFREHTVGYFVIRNAVYLMEKQYLFQ
                     IINVLTSAMENLHKKERLAYLNQVLSELYVKDAMTGMYNRLGYQKLACRMFEKKKQTG
                     ENLSIIFMDMDRLKSINDEFGHIYGDWAIKTIASAILKYIPEGAVAVRSGGDEFLVVL
                     EQTADAEIGKIIGEIRKDIRERSAEMKLPFPLTVSAGCVHTDMTTGCDLDDYVREADA
                     IMYEEKTAKKANRT"
     gene            2487..3272
                     /locus_tag="ACZO9R_03010"
     CDS             2487..3272
                     /locus_tag="ACZO9R_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0912076.1"
                     /translation="MKIERERVRETFREYTDAYDATDEKIKLKIDHTYRVAELCERIA
                     KAEQMEKTEVDLAWLLGMLHDVGRFEQLRRYGTFSDADSIDHAALGADILFGGPLSAG
                     KGLIRTYVDDAAEDEVIETAIRVHSAYRIPEGLAPRTEKLCHILRDADKIDILQVNVD
                     VPLEEIYNTTTEELRNAAVTPAVMDSFYEHHATLRSIKRTPVDHVVGHISLVFELVFP
                     ESVRIVKEQGYLEKLLHFESRNAATNAQFAELRVEMERYLKGR"
     gene            complement(3259..3855)
                     /locus_tag="ACZO9R_03015"
     CDS             complement(3259..3855)
                     /locus_tag="ACZO9R_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HIR0912077.1"
                     /translation="MKKHDRLPDFSKLCAPEQTPISFFLSQQIYFFFLGSIGGFLWEV
                     AIFLYRDKTFANRGFLYGPWLPVYGIGAVLFYLLLGCRRKKIHPASVFFLSAAIGTGL
                     ELLIGWFLDAFWNLRYWDYSGYPFHFHGYICLYAAVGFGIAGVLWICVLEAPLTRLWK
                     RLSPKVRLGLNTLLLLLFAADCAAALIFPNRGAGITFP"
     gene            4015..>4644
                     /gene="guaA"
                     /locus_tag="ACZO9R_03020"
     CDS             4015..>4644
                     /gene="guaA"
                     /locus_tag="ACZO9R_03020"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR0912078.1"
                     /translation="MNNNFSQEKVIVIDFGGQYNQLVARRVRECNVYCEIYSYRTDIE
                     QIKAMNPKGIILTGGPNSCYEPDSPTYTKELFELGIPVLGLCYGAQLMMHVLGGKVEK
                     APVREYGKTEVFVDTTSPLFGDVSEKTICWMSHFDYISKVAPGFSIAAHTADCPVAAA
                     ENREKKLFAIQFHPEVLHTQEGTKMLHNFVRGVCGCAGTWRMDHFVEHTI"
BASE COUNT         1257 a          955 c         1412 g         1020 t
ORIGIN      
        1 gaaaatggtc tcttatcatc tgacgaaagg agaacaactg gaagtaattt tgaacagtac
       61 attcaagcaa ctggaagata tcacggatgt tcatgaaatg acgaatgcac agttgaaacg
      121 cctgattaac aaaattgaag tggacaagga tggtaatgta gatatttacc tgcgtcttat
      181 tggtgatttg ggattggatg aaactgtctt aattgaagga gacaaaaaca gagaggaaaa
      241 agacaataaa acagctctaa attgtcacaa ccatacataa ggacgtcacc gagcgccttg
      301 cgcaggtcaa cccgtcgctc gcggcggaga cgcgcaaggt gctggacatc aacaagtccg
      361 agcggcacat ccgcggcggg atggcgacaa aggagaagta tctgcatcag catgtgtgaa
      421 aacgcagaag catttgacgg catgaaaacg tttttatata ataggagaag ataacggcag
      481 gatagatgga ggataagcgc agatgaagaa gatagcgttg atcatggacg gatggaagcg
      541 attcttcaca tatgcatggc ccgccggagt gttggagcgt atcagagaga cgaatgagga
      601 cgtgaatctg tatattttca acagttccgg agactggagc agggatgagg attacaatat
      661 cggagagtac aacatctatc ggctgccgga cttaaacgat ttcgacggca ttattgtaga
      721 tctgaacaat atccgctatt ccgaggtgcg ggagtatgtg atctcctcgg caaaggccac
      781 cgggaagccg gtgatttcta ttgcaaatga gattgcggat ttttactatg tcggtattga
      841 caactattcc gccatgcagg aaatagcggc gcatctctat gaggtgcata attgcagacg
      901 cttctggtat gtcatgggac cggacgataa ttacgagagc aaccggcgtg tggctgcgtt
      961 gcgcgatttt tcagagacac acgggatttt gtgggatgcc agggatctct actgcgagag
     1021 ttatgaatac aagtgtggag tgcatggatt tgaaaaattg ctcacgctgc acgggaaact
     1081 tccggatgcc attatctgtg ccaacgataa cattgccgtc ggcgtgtgtg agaccgcgga
     1141 agctcacggc tacaaggcac ccgacgattt tctggtgacc ggattcgata attttgacaa
     1201 ggcgagctat tattccccgc acatcacaac ggtcggacat atccgcgagc aggtgggata
     1261 ccgttgtgcg gatattcttc tgcggctgtg gaggggcgag acggtaccgc ggtttaatta
     1321 tacggggcac cagtgcattt tctgggaaag ctgcggctgc gacgccggca ttgcggtgga
     1381 tcaggcagaa cattccaggg cgcagattgt ctatggaatc gagacggacg aatttgagga
     1441 gcaggtgctt tcgctggagt atgaattgtt gcagtgcgag acggtgcgtg agatgtcgcg
     1501 ctggattccg aaatgtattc cggctatgcg gtgtgacgcg atgtatctga tcatggatga
     1561 gcacatgaat gatttccggg aattgagcga ttattatgac agacatctga ttgaggatga
     1621 agagttctgc gtgcacggtt atccggagaa aatgcagatg gagttcgcct atgaggacgg
     1681 tgtggtaaaa gagagcgagg agacggtcgt ggaggggatt ttcccgacct ttgattacgc
     1741 ggagggcgga aaagattttc tgtttctgcc gcttcatttc cgggaacaca cggtgggata
     1801 ttttgtcatc cggaatgctg tgtatctgat ggagaagcag tatttgttcc agattatcaa
     1861 cgtgttgacg tctgcgatgg agaatctgca taaaaaggag cgccttgcct atctgaatca
     1921 ggtgctgtca gaactgtacg tgaaggatgc catgacggga atgtacaatc ggctcggtta
     1981 ccagaagctg gcgtgccgta tgtttgagaa aaagaagcag acgggggaga acctttcgat
     2041 tatttttatg gatatggaca ggctcaaaag catcaacgat gaattcgggc atatctatgg
     2101 ggactgggcg atcaagacga ttgcgtcggc gattttaaaa tatattccgg agggagcggt
     2161 cgcggtccgg agcggcgggg atgagttcct tgttgtacta gagcagaccg cggatgcaga
     2221 gatcgggaag atcatcggag agatccggaa ggatatccgg gaacgttcgg cggagatgaa
     2281 actgccattt ccgctgacgg tcagtgcggg atgtgtccac acagatatga cgactgggtg
     2341 cgatctggac gactacgtgc gcgaggcgga tgccatcatg tacgaggaga agaccgcgaa
     2401 aaaagcgaac cgcacctaag caggatgaaa aggcttccaa ccgggcaggg gaaaagaata
     2461 agaaagtcag aaggagatac cggcagatga aaatcgagcg tgaacgggtc agagaaacat
     2521 tccgggagta tacggatgcc tatgatgcga ccgatgagaa aataaaattg aaaattgacc
     2581 acacttaccg ggtggcggaa ctgtgcgagc ggatcgcgaa ggcagagcag atggagaaga
     2641 cagaggttga tctggcatgg cttcttggaa tgctgcacga tgtgggaaga tttgagcagc
     2701 ttcggagata tggaacattc agtgatgcag attccattga ccatgcggca ctcggagcgg
     2761 atattctgtt cggtggtccg ctgtctgcgg ggaaaggtct gatccgcacg tatgtggacg
     2821 atgcggcgga ggatgaggtg attgagactg ccataagagt gcacagtgcc taccggattc
     2881 cggaggggct tgcgccgcgc acggaaaagc tgtgccatat cctgcgggac gccgacaaga
     2941 tcgatattct gcaggtcaat gtggatgtgc cgctggagga gatttacaat accacgacgg
     3001 aggaactgcg caacgcagcc gtcacaccgg cagtgatgga tagcttttac gaacatcacg
     3061 cgacactgcg cagcatcaag cgcacgccgg tggatcatgt ggtggggcat atctcgctgg
     3121 tgtttgaatt ggtatttccg gagagtgtgc ggattgtaaa agagcagggt tatctggaaa
     3181 agctgctgca ttttgagagc cgcaacgctg cgacaaacgc gcagttcgca gaacttcggg
     3241 tggagatgga gcgctatctt aagggaaggt aatgcctgct ccgcggtttg gaaaaatgag
     3301 cgcagccgcg cagtctgcgg caaagagcag cagaagcagg gtgtttaatc caaggcgcac
     3361 tttgggggag agacgtttcc agagtctggt gagcggcgcc tccagcacac agatccagag
     3421 cacgcccgcg atgccgaaac cgaccgccgc gtacaggcag atatagccat gaaaatgaaa
     3481 aggatagccg ctgtaatccc agtagcgcag attccagaag gcatccagaa accatccgat
     3541 caggagttcc agaccggtgc cgatggcggc actgaggaaa aagacgctgg ccgggtggat
     3601 tttttttcgc cggcagccca gcagcagata gaaaagcact gcgccgatgc cgtagaccgg
     3661 aagccacggc ccgtagagga agccgcggtt ggcaaaggtt ttgtcccgat acagaaagat
     3721 ggcaacctcc cagagaaacc cgccgatcga gccgagaaag aagaaataaa tctgctggga
     3781 gaggaaaaac gatataggag tctgctccgg tgcgcagagt ttggaaaaat ccggaaggcg
     3841 gtcatgtttt ttcatggaga tagtataacc ggaacattga aaattattga attgtgaaat
     3901 ggtggaaaat atagaaatgc gccttgtaaa ttgcgggagc gtatcttata atagtagcaa
     3961 ttgagcaaac cgccctttgc ggcggaaaaa catcaggagg aaaagagtgt ggaaatgaac
     4021 aacaattttt cacaggaaaa agtcattgtc atcgactttg gtggtcagta caaccaactg
     4081 gttgccagac gtgtcagaga gtgcaatgtt tactgtgaga tctattccta ccgtacagac
     4141 atcgagcaga tcaaggcgat gaatccgaag ggtatcattt taaccggcgg tccgaacagc
     4201 tgctacgagc cggattctcc gacgtacacg aaggaactgt ttgaacttgg aattccggtt
     4261 ttgggactct gctacggtgc gcagcttatg atgcatgtgc tgggcggaaa ggttgaaaag
     4321 gcaccggtgc gtgagtatgg caagacagag gtgtttgtcg atacgacatc gccgcttttc
     4381 ggcgatgtgt cggagaagac gatctgctgg atgagccatt ttgattatat ttcaaaagtt
     4441 gctccgggat tttccattgc ggcgcacacg gcagactgtc cggtggcggc agcggagaac
     4501 cgcgagaaga agctttttgc aatccagttc catccggaag tgcttcacac ccaggagggc
     4561 actaagatgc tgcacaattt cgtgcgcggt gtctgcggtt gtgccggaac ctggcgcatg
     4621 gatcattttg tggagcatac gatc
//
LOCUS       DBMDQJ010000098         4636 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7082_length_4636_cov_1.500772, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000098 DBMDQJ010000000
VERSION     DBMDQJ010000098.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7082_length_4636_cov_1.500772"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(49..657)
                     /locus_tag="ACZO9R_03025"
     CDS             complement(49..657)
                     /locus_tag="ACZO9R_03025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0912079.1"
                     /translation="MIKNVIFDVGQVLVAWEPVEAMRALGMDEETVRAVADATVHTSD
                     WDEADRGALSDEELLTGFIRKAPAYEAQIRNFWEHVNRAIYPFPYVGEWMRTLKDKGY
                     RLYILSNYGAWTYAKTKDQSLGFLKDVDGALFSFEVKQIKPEPEIYHSLLERFHLRAE
                     ECVFLDDRQNNVDGAIACGISAIRFTGYEDARQKLKEYGVEC"
     gene            complement(668..1948)
                     /locus_tag="ACZO9R_03030"
     CDS             complement(668..1948)
                     /locus_tag="ACZO9R_03030"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR0912080.1"
                     /translation="MVTAVVGANWGDEGKGKITDMLADEADIVIRFQGGANAGHTIVN
                     NYGKFALHTLPSGVFHSHITNIIGNGVALDIPKVIGELKSITDRNVPAPKLMISDRAQ
                     MVMPYHVLFDQYEEERLGKNSFGSTKSGIAPFYSDKYAKIGFQVSELFDEELLKEKVK
                     RTCETKNIMLEHMYHKPLLKEEELLATLHEYRDMVAPYVGDVSLFLDQAIKDGKRILL
                     EGQLGTLKDPDHGIYPMVTSSSTLAAYGAIGAGIPPYEIKKIVTVCKAYSSAVGAGAF
                     VSEIFGDEADELRRRGGDGGEFGATTGRPRRMGWFDCVASKYGCRMQGTTDVAFTVLD
                     VLGYLDEIPVCVGYEIDGEVTTDFPVTAKLEKAKPVLKKLPGWKCDIRGIKKYEDLPE
                     NCRKYVEFVEEQIGYPITMVSNGPGREDIIYRSK"
     gene            complement(2219..3115)
                     /locus_tag="ACZO9R_03035"
     CDS             complement(2219..3115)
                     /locus_tag="ACZO9R_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0912081.1"
                     /translation="MDINLEYYKIFYYVAGSQSITLAAEQLAISQPAVSQAVKHLEQA
                     LSCPLFVRTTKGVRLTKEGEMLYSYVQRGYETILSGERKLSEMLNLEQGEICIGASDM
                     TLKYFLLPYLEEFHERYPGIRVTVTNAPTPETIRHLCDGRIDFGIVSTPVEKRASLKC
                     VPVKEIRDVFVAGRKFEYLKERMLGYEELMKLPVMCLEGNTSTRTYVEQFLASKQVTI
                     RPEFELATSDMLIQFAVRNLGIASVVRNFAEEHLTEGDLFELRFLEEIPARTFCIVAN
                     ERIPLSAAAATLMNVLVQGTSQ"
     gene            3250..4593
                     /locus_tag="ACZO9R_03040"
     CDS             3250..4593
                     /locus_tag="ACZO9R_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912082.1"
                     /translation="MTKDMTTGSPLRIILLFSIPVLLGNLFQQFYNMVDTIIVGRYLG
                     EEALAAVGSTGCLMFLVLGFANGIAQGFGVMVSHAFGAKDFKLLRHYVALSLILTLII
                     SLLLTIPTVTASRLLLAWMHTPENIIAMADAYIKVIFAGILLTMAYNVLSGILRGIGD
                     SRTPLYFLILSSVLNIILDVVLIVYTGLGTAGAAYATIIAQGVSAVLCFFYMYRKYDI
                     LRTRHEDYYLDSAGVKNMLAIGVPMALNYSITAIGTMIMQSAVNVFGSSVVAAFTAAS
                     KVSNIATQSMPTLGTAMATYCGQNLGAGKYDRIYAGMRQGLFLCFGAAATAAAICIFG
                     GRFIVSWFVSNPSEEIFSSAMQYLTIASWFFLPLAMIFLYRNALQGLDQGLIPMLSGV
                     VELACRFGVIALIPASTGFFGVCFADPAAWAGAGIPLFITYLLWEHRMKQCSMAV"
BASE COUNT         1011 a         1369 c         1039 g         1217 t
ORIGIN      
        1 ttacttatca gcccaaagca cgatatgctt cttgcgacag ttccctttct agcactctac
       61 accgtattct tttaatttct gtctggcatc ctcgtagccg gtaaaacgaa tggcagagat
      121 gccgcaggcg atggcaccgt ccacattgtt ctgtctgtcg tcgaggaaca cgcactcctc
      181 cgcgcgcaga tgaaagcgct caagcaggga gtgataaatc tccggctccg gcttaatctg
      241 cttcacctcg aaggaaaaca atgctccatc cacgtcctta aggaagccaa gcgactgatc
      301 cttcgtcttc gcgtatgtcc aggcaccgta attggaaagg atatacaggc gatacccctt
      361 gtcttttaac gtccgcatcc attccccgac atacggaaac ggataaatcg cccggttcac
      421 atgctcccag aaattgcgga tctgtgcttc gtatgccgga gcttttctga taaagcctgt
      481 gagaagttcc tcatcggaca gggctccccg gtccgcctcg tcccagtcac tggtgtgcac
      541 cgtggcatcc gcgactgcgc gcaccgtctc ttcatccatt ccaagcgcgc gcatcgcctc
      601 caccggttcc catgccacca gaacctgtcc cacatcaaaa atgacattct taatcatatg
      661 cgtcatccta cttgctgcgg tagataatat cctctctgcc cggtccgttt gataccatcg
      721 tgattggata tccgatctgc tcctccacaa actctacata ctttctgcag ttctccggca
      781 gatcttcgta tttcttgatg ccgcggatat cgcacttcca gcccggaagc ttcttaagta
      841 ccggctttgc cttctcaagc tttgctgtca ccgggaaatc tgttgtcact tcaccgtcga
      901 tctcatagcc tacgcatacc ggaatctcat ctaagtatcc caatacatcg agcacagtaa
      961 atgcaacgtc ggttgttccc tgcatacggc agccgtactt gcttgccaca cagtcgaacc
     1021 atcccatacg tctcggacga cctgttgttg cgccgaattc tccgccgtca ccgccgcgtc
     1081 ttctaagctc gtctgcctca tccccgaaaa tctcgctgac gaatgcgcct gctccgactg
     1141 cggaagaata agccttgcat accgttacga tcttcttgat ctcatacggc gggatgcccg
     1201 ctccgattgc accataagct gccagcgtgg aggaggatgt taccatcgga tagattccgt
     1261 gatccggatc ctttaaggtt ccaagctgtc cctctagcag aatgcgcttg ccgtctttga
     1321 ttgcctgatc gaggaataag gagacgtctc cgacatacgg agcaaccata tccctgtact
     1381 catgtaaggt tgcaagaagt tcctcttcct ttaataacgg cttatgatac atatgctcca
     1441 gcatgatgtt cttcgtctcg caggtgcgct ttaccttttc ttttaagagc tcctcatcga
     1501 acagctcgct gacctggaaa ccgatctttg catacttgtc cgaatagaac ggtgcgattc
     1561 ctgactttgt ggaaccgaaa gaattcttgc cgagacgctc ctcctcgtac tggtcgaaca
     1621 ggacatgata cggcataacc atctgtgctc tatccgagat catcagcttc ggtgccggta
     1681 cgttgcggtc tgtgatcgac ttgagctcgc cgattacctt cgggatgtcg agtgcgacac
     1741 cgtttccgat aatatttgtg atatggctgt ggaatacgcc ggacggcaat gtgtgcagtg
     1801 caaacttgcc ataattgttc acaatggtgt gtcctgcatt tgctccgccc tgaaaacgga
     1861 tgacgatgtc agcctcatcc gcgagcatat ctgtgatctt gccttttccc tcatcgcccc
     1921 agttagcgcc tactactgct gtaaccatat gtttttcctc ctgatatgat acactttttt
     1981 gccggaagtc cgcattttcc tgattccagg ctgcatccat gcggccggtc tgtccgaaag
     2041 cagcatccac tctgtaaatc tgcctcatga cataaaaagt gacggttgta aaaaaccgtc
     2101 actcacttcc gcctttttac ttattgatta tacacgaacc ttttgcataa gtaaaatcaa
     2161 tattcattat atgtggcata agtatttatt atgcctgttt ctaaaagaaa atccctgctt
     2221 actgtgatgt cccctgtacc agaacattca tcagcgtagc cgctgcggct gacagcggaa
     2281 tccgttcatt tgcaacaatg cagaacgtac gcgccggtat ctcctccaag aaccgcagtt
     2341 caaacagatc tccctccgtc aggtgctcct cggcaaaatt tctgaccaca cttgcaattc
     2401 caaggttgcg caccgcaaac tggatcagca tatcgctggt ggcaagttca aactccggcc
     2461 gaatcgtcac ctgcttcgac gcaaggaatt gctccacata cgtcctggtc gaggtatttc
     2521 cctccagaca catgaccggc agcttcatca gttcctcgta ccccagcatg cgctccttta
     2581 aatattcaaa cttcctgcct gccacaaaca catcccggat ctccttcacc ggaacgcatt
     2641 tgagcgaagc tctcttctcg accggcgtgc tcacgatgcc aaaatcaatg cggccgtcgc
     2701 agagatggcg gatcgtctcc ggggtcggcg cattcgtcac cgtcacgcgg attcccgggt
     2761 agcgctcatg aaattcttcc agatacggaa gcaggaaata ctttaacgtc atgtcgctgg
     2821 ccccgataca gatttcgccc tgttccagat tcagcatctc agagagcttc ctctcgcctg
     2881 agaggatcgt ctcatagccg cgctgcacat aggaatagag catctccccc tccttcgtca
     2941 gccgcacgcc cttggtcgtc cgcacaaaca atgggcacga aagcgcctgt tctaaatgct
     3001 tcaccgcctg gctgactgca ggctgtgaga tggcgagctg ctccgctgcc agtgtgatgc
     3061 tctgggagcc tgccacataa taaaaaattt tataatattc cagattgatg tccatggatc
     3121 tctcctggga aaaaagtctc atttttttac aaaaacggat tgtattttcc actgtcatga
     3181 agtatactat gccttacgca aagtaccagt atctctacta taacagacta tgaagggaga
     3241 cgcaatccaa tgaccaaaga tatgaccacc ggcagcccgc ttcggattat cctgctattt
     3301 tctattccgg ttctgctcgg caatctgttc cagcagttct ataatatggt agatacgatt
     3361 attgtcggac gctatctcgg cgaggaggct ctcgccgccg tcggctcaac cggctgcctg
     3421 atgtttctcg tgctcggttt tgccaacggc atcgcccagg gcttcggcgt catggtttca
     3481 cacgctttcg gagccaaaga ttttaagctg ctccgtcact acgtggcgct atccctgatt
     3541 cttacactga ttatatcgct tctgctgacc atcccaacgg tgacagcttc ccgcctcctt
     3601 ctggcatgga tgcacacacc ggaaaatatt attgccatgg cagacgctta tattaaggta
     3661 atttttgcgg gcattctgct gacaatggcg tacaacgtgt tatccggcat cctgcgcggc
     3721 atcggcgaca gccgcactcc tctctacttc ctgattctct cgtccgtgct caacatcatc
     3781 ttagatgtcg tactgatcgt gtacacaggg cttggaactg ccggagccgc ctacgcgaca
     3841 atcatcgcac agggagtctc cgccgtgctc tgcttctttt atatgtaccg gaaatacgat
     3901 atcctgcgca ccagacatga ggattattat ctggattccg ccggcgtaaa aaacatgctc
     3961 gccatcggtg tgccgatggc gctcaactac tcaattaccg ccattggcac catgattatg
     4021 cagagtgcgg tcaacgtgtt tggctcgagt gtggtcgccg ccttcaccgc ggcctccaag
     4081 gtcagcaata tcgcgaccca gtccatgccg acactcggca ccgcaatggc aacctactgc
     4141 ggacagaacc tcggtgccgg caaatacgac cgcatctacg ccggcatgcg gcagggactt
     4201 ttcctctgct tcggtgcggc agccacagcc gccgcgatct gcatctttgg cggacgtttt
     4261 attgtgagct ggttcgtgag caatccttcc gaggagatct tctcttccgc catgcagtac
     4321 ctgacgatcg caagctggtt ctttctcccg ctcgccatga tctttttata ccgcaatgcc
     4381 ttacagggcc tagatcaggg actgatcccc atgctctctg gtgtcgtcga gctcgcctgc
     4441 cgcttcggcg tcatcgctct cattccggca agcaccggct tcttcggtgt ctgcttcgct
     4501 gatccggctg cctgggccgg tgccggcatc ccgctgttta tcacctatct tctctgggag
     4561 caccggatga agcagtgttc catggcggtg taaacatttt tccctcacac ggaaatgcat
     4621 cctattgcat caaaaa
//
LOCUS       DBMDQJ010000099         4623 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7099_length_4623_cov_1.341663, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000099 DBMDQJ010000000
VERSION     DBMDQJ010000099.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4623)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7099_length_4623_cov_1.341663"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(192..479)
                     /locus_tag="ACZO9R_03045"
     CDS             complement(192..479)
                     /locus_tag="ACZO9R_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912083.1"
                     /translation="MDEMDDGDEERLPGEPMMTAVTLESDLGEILQFQMPHNWLLMQG
                     LQEGEEWPEDIEEEDSEALKAREQAAWMENYYHALQELEGEEEQCKHGAEG"
     gene            complement(1062..2270)
                     /locus_tag="ACZO9R_03050"
     CDS             complement(1062..2270)
                     /locus_tag="ACZO9R_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HIR0912084.1"
                     /translation="MYNVRKVTEDIVWVGASDRRLALFENIFPIPRGVSYNSYVLLDE
                     KTVLLDTVDASVAGQFFENLEHVLDGRKLDYLIVNHMEPDHCAMIGDLVRRYPEVQVV
                     GNTKTFGMIKQFFGTDFAERAVTVKEGDTLATGAHTLHFVMAPMVHWPEVMVTYDEKD
                     KVLFAADGFGTFGALNGNIFADEVDFDRDWLDDARRYYTNIVGKYGASVQALLKKASG
                     LEIAVICPLHGPIWRENLGYILEKYQKWSTYEAEDQAVVILYATMYGNTASAADALAG
                     RLAAKGVKKIAVYDVSNTHVSELISEIFRASHVVFAAPTYNGGIYPVMENLLADMKAL
                     AVQNKTVALMENGTWAPMTAKQMREKLAELKNVTILDTQITIKSAMAPEQEGQLEALA
                     DAIVASMDNQ"
     gene            complement(2540..>4623)
                     /gene="secA"
                     /locus_tag="ACZO9R_03055"
     CDS             complement(2540..>4623)
                     /gene="secA"
                     /locus_tag="ACZO9R_03055"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008564 - protein-exporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR0912085.1"
                     /translation="DAYNCDITYVTNNELGFDYLRDNMVIYKEQLVQRGLHFAIIDEV
                     DSVLIDEARTPLIISGQSGKSTRLYEACDILACQMKRGEDMPEYSKMDAIMGVEQEET
                     GDFIVNEKDKVVSLTQDGVKKVEQFFKIENLADPENLEIQHNIILALRAHNLMFRDQD
                     YVVKDDQVMIVDEFTGRIMPGRRYSDGLHQAIEAKEHVKVKRESKTLATITFQNFFNK
                     FEKKAGMTGTALTEEKEFRDIYGMDVVEIPTNRPIARIDHHDVVYKTKNEKYKAVVEE
                     VVKSHEKGQPVLVGTINIDTSELISGMLKREGIPHTVLNAKFHEKEAEIVAGAGQHGA
                     VTIATNMAGRGTDIKLDDESKAAGGLKIIGTERHESRRIDNQLRGRSGRQGDPGESQF
                     FISLEDDLMRLFGSEKLMDIFNALGVPEGEQIQHKMLTSAIEKAQKKIEGNNFGIRKN
                     LLEYDQVMNDQREIIYKERRRVLDGENMRDAIFKMITDRVENCVDTCISSDIPKEEWD
                     LNELNQLILSMIPMQAVTPEDVDSVKNNKELKHLLKERAVKLYEAKETEFPEPEQFRE
                     LERVVLLKVIDRKWMDHIDDMDQLRQGIGLQSYGQRDPLVEYKMAGFDMFDEMTASIQ
                     EDTIRLLYHVKIEQKVEREQVAKVTGTNKDESATNTPKKRAAAKVYPNDPCPCGSGKK
                     YKQCCGRNAAQ"
BASE COUNT         1056 a         1387 c          878 g         1302 t
ORIGIN      
        1 cttcttttct gctgacacag gtactccaac aaagtgatac atttctatat tttcaacatc
       61 acctacctca aaaggtaaat caatattgac tgtcttgttg ttgcacccaa ccaagccaag
      121 cacacaaacc actgctaaaa ctaatgctat caactttttc atacaatcaa cctccttcgt
      181 caaattccga tttatccctc tgccccatgc ttacattgct cttcttcccc ctcaagttcc
      241 tgcaacgcat gataataatt ttccatccac gctgcctgtt cccgcgcttt taacgcttct
      301 gaatcctcct cctcgatgtc ctcaggccac tcttctcctt cttgtaatcc ctgcataagc
      361 aaccagttgt gaggcatttg aaattgaagt atttctccca gatcactttc caacgtaacc
      421 gctgtcatca tcggctcgcc cggcaagcgt tcctcatctc catcatccat ttcgtccatt
      481 cgttgtattg tccacatttc aaagttcctc tatcccagtg ataagcccct caaaaatatc
      541 acgttcatat tacgctccgg caaaagtccg tgaaacctcc ggttccccgg actcgcgggc
      601 gcgttcacat ggcgcatatc cgtctgccgc atgcaagcat gcgcaaccgt ctctgttcat
      661 acccgcagtg ctcgtccgaa aaatcataga ttttccggac tgacgggcat tcgccctatc
      721 atccgcaaca tctgcgatat atagcctgca agcagacata tatcgcagcc gtcacgtctg
      781 gcactgctca ttgcatatgc acattatatc acatcttttt ttaattgcaa acaaatgttc
      841 gcttttgtta taaaaagagt tctctccaca gaatgcattt tcttataaag cagaaaaaga
      901 cacccatcca cttcgtacat attactttgt acaagcaaac aggtgccttt tcccaaccag
      961 cgatcctgaa acgatcgcgt tatttcttct tttgttatcc gttatttata tgagaaagtc
     1021 tttgcttccc acaaaacgca atgataaagt cagcaacaca attactgatt gtccatgctc
     1081 gctacgatcg catccgcaag tgcctcaagc tgtccttcct gctccggcgc catcgccgat
     1141 tttatggtga tctgcgtatc cagaatcgtc acatttttca gttccgccag tttttctctc
     1201 atctgctttg ccgtcatcgg tgcccaggtg ccgttctcca tcagtgctac ggtcttgttc
     1261 tgaaccgcaa gtgccttcat gtcggcgagc aggttctcca tcaccggata gatgcctccg
     1321 ttgtaagtcg gcgccgcaaa caccacatgg cttgcgcgga agatctcact gatgagctcc
     1381 gacacatggg tgttcgagac atcataaacg gcaatcttct tcacgccctt tgccgcaagt
     1441 cttcctgcaa gcgcatccgc cgccgatgcc gtgttaccgt acatggttgc atacagaatc
     1501 acaaccgcct gatcctccgc ctcataggtg ctccacttct gatatttttc cagaatatag
     1561 cccagattct ccctccagat cggtccgtgg agcgggcaga tcactgcgat ctccagcccg
     1621 gaggctttct ttaacaatgc ctgcaccgat gcaccgtact ttccgacaat gttcgtgtaa
     1681 tatcttctgg catcatccag ccagtcgcgg tcaaaatcga cttcatccgc aaaaatattg
     1741 ccgttcaatg cgccaaacgt tccgaatccg tccgcagcaa aaagcacctt gtccttctca
     1801 tcataggtta ccattacctc cggccagtgt accatcggcg ccatcacaaa gtgcaatgta
     1861 tgcgcaccgg ttgcaagcgt atctccctct tttaccgtca cggctctctc cgcgaaatcc
     1921 gttccgaaga actgttttat cattccaaat gtcttggtgt ttccgaccac ctgcacttcc
     1981 ggataacggc gcaccagatc cccgatcatc gcacagtgat ccggttccat gtgattgaca
     2041 atcagataat ccagtttccg tccgtcgagc acatgctcca gattttcaaa aaactgtccc
     2101 gccacggacg catcgactgt atccaaaagt accgtcttct catccaataa tacataagag
     2161 ttgtaggata ccccgcgcgg aattggaaat atattttcaa acagcgccag tcttctgtca
     2221 ctggctccaa cccaaacaat atcctcagtc acttttctta cgttgtacat agtgttcctc
     2281 cttctgtccg gcagtctgcc gtctcatatt ctacctgttt ggtatgaatt ataaatcaga
     2341 tttctggaaa ttgccaccca aaaatgcact ctgtatgtat ttttctgcat acagatgaaa
     2401 atatgcaaaa agagcattcc cggcaaaaat tatctacctg aaatgctctc tcttccttat
     2461 gctgttctgt cgacttttct gccggataaa cagcgttctg ccgtccgccc gtcattgcag
     2521 tctgccgttt gcagcactcc tactgcgctg cgtttctgcc gcagcactgc ttatacttct
     2581 tgccgcttcc acacggacac ggatcgttcg gatatacctt tgccgcagca cgcttcttcg
     2641 gcgtgttcgt cgcagactca tccttgttcg ttccggtcac tttcgctacc tgctcacgct
     2701 caaccttctg ctcaatcttc acatgataca gcagccggat cgtatcctcc tgaatgctcg
     2761 ccgtcatctc gtcgaacatg tcaaatcctg ccatcttgta ctcgacaagc ggatctctct
     2821 gtccgtagga ctgcagtccg ataccctgac gaagctgatc catatcatca atgtgatcca
     2881 tccacttgcg gtcgatcacc ttcaggagaa cgacacgttc aagttcacgg aactgctccg
     2941 gctcagggaa ctccgtctcc ttcgcctcat acagcttcac tgcacgctcc tttaacagat
     3001 gcttcagttc cttattattc ttaacgctgt ccacgtcctc cggcgtcact gcctgcatcg
     3061 gaatcatgga gaggatcagc tggttcagtt cattcagatc ccactcttcc ttcggaatat
     3121 cagaagaaat acaggtatct acacaatttt ccacacgatc cgtgatcatc ttgaaaatcg
     3181 catcgcgcat gttctctccg tccagtacgc ggcggcgctc cttgtagata atctctctct
     3241 gatcgttcat aacctgatca tactcaagaa gattctttct gataccgaag ttgttgccct
     3301 cgatcttctt ctgcgctttc tcaatcgcag atgtcagcat cttatgctgg atctgctcac
     3361 cttccggaac accgagtgca ttgaaaatgt ccatcagctt ctcagaaccg aacagacgca
     3421 tcagatcgtc ttcaagagaa atgaagaact gggactcacc cggatctccc tgacgtccgg
     3481 aacgaccgcg cagctggtta tcaatacgtc tggattcatg acgctctgta ccgatgatct
     3541 tcaaaccgcc tgcagccttc gactcatcat ccagcttaat atcggtacca cgtcctgcca
     3601 tgttggtcgc gatcgtcacc gctccgtgct gtccggcacc ggctacaatc tcagcctctt
     3661 tttcatggaa ctttgcgttc aataccgtat gcggaattcc ctcgcgcttc agcatgccgc
     3721 tgatcagctc ggaagtatcg atattgatgg taccgacaag taccggctgt cccttttcat
     3781 gggattttac cacttcctcc acaaccgcct tgtatttttc attctttgtc ttgtatacaa
     3841 catcatgatg atcgatacgg gcgatcgggc ggttggtcgg gatctcgacg acgtccatgc
     3901 cgtagatgtc gcggaactcc ttctcctccg tcaatgcggt accggtcata cctgctttct
     3961 tctcaaactt gttaaagaag ttctggaatg tgatcgttgc cagagtcttg ctctctctct
     4021 tgaccttcac atgctccttc gcctcaatcg cctgatgaag tccgtctgaa taacggcgtc
     4081 ccggcataat acgtccggta aactcatcga cgatcataac ctggtcgtcc ttcaccacgt
     4141 aatcctggtc gcggaacatc aggttgtgtg cgcgcagcgc cagaataatg ttatgctgaa
     4201 tctccagatt ctccggatca gccaggttct ctatcttgaa gaactgctca accttcttca
     4261 caccatcctg tgtcaggctg accaccttat ccttctcgtt tacgatgaag tctccggtct
     4321 cctcctgctc cacgcccatg atagcatcca tcttggagta ctccggcata tcctcaccgc
     4381 gcttcatctg gcacgccaga atatcgcatg cctcatacag tctggtggat ttgccgctct
     4441 gtccggaaat gatgagcggt gtacgcgcct catcaatcaa taccgagtcg acctcatcga
     4501 tgatggcaaa atgcagtccg cgctgcacca gctgttcctt atagattacc atgttatcac
     4561 gcagataatc aaagccaagc tcattgtttg tcacgtaggt aatgtcacag ttataagcat
     4621 ccc
//
LOCUS       DBMDQJ010000100         4595 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7160_length_4595_cov_1.543391, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000100 DBMDQJ010000000
VERSION     DBMDQJ010000100.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4595)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4595)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4595
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7160_length_4595_cov_1.543391"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="ACZO9R_03060"
     CDS             <1..516
                     /locus_tag="ACZO9R_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912086.1"
                     /translation="LARYHTGHCSKKQLLLGITGSTALIFCADGALTLHQGVIYSTFS
                     RDCSIFIIFTAVLLLLLFREFSLQSRIINRAATNVLAIYVLDQVIRSLLGRYLDLNAF
                     SGQWYLFFVVCGYVLLVMLLAAVCNELRKLTIGRIEPWLAGIIAAFLQACGALLIRTG
                     QRIVHWFISMD"
     gene            557..1498
                     /locus_tag="ACZO9R_03065"
     CDS             557..1498
                     /locus_tag="ACZO9R_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912087.1"
                     /translation="MKKRFLYLRYYLKHLHHKFRAKRGGMYSIMHTKFLGKMPPSDEE
                     ANHLIKEMITSGKPFALCRLGSAEFTLMQLFDEYQLFHHDRLHQSNMYTLFHKDMDEI
                     ARWVNLTKEDCRDVDIMAYFEDHPMEEYLIRSYCQNSRLIRLEQIETILYDVPWTMAL
                     EGKKVLVISPFVDTIREQYPKIDKIYEGRKMLPSFELKTLKSVWFSGDREDDDFDTWF
                     DALQYLYDEAMKIDFDIALLSCSTFGFDLACMFKRAGKQAIQYGGALQVLFGIKGARW
                     DNYPPYSKYYNEYWVRAPKTEAPSKRFANKLDNGCYW"
     gene            1537..1929
                     /locus_tag="ACZO9R_03070"
     CDS             1537..1929
                     /locus_tag="ACZO9R_03070"
                     /inference="COORDINATES: protein motif:HMM:NF024859.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR0912088.1"
                     /translation="MNVDHIGYAVKNIDASRQAFEELGYVFDPVVNDTDRNIFISFGR
                     LGDYRIELVSPLDPALPSPVDGTLKKSGNTPYHICYTSSRFEDDIKELQQKRFLLTIP
                     PAPACAFDGRRVAFLYHLQIGLLELVEA"
     gene            complement(1926..2489)
                     /locus_tag="ACZO9R_03075"
     CDS             complement(1926..2489)
                     /locus_tag="ACZO9R_03075"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR0912089.1"
                     /translation="MATSFYSDEELQQMGFAHVGHDVMISRKASIYGASNIWIGNHVR
                     VDDFCVLSGKITFGNYIHVAVTTTLFGGTAGITFEDYTGISSHCAVYAASDDYSGDFM
                     TNPTVPEKFTNVIEEPVVIKKYSNIGAGCIILPGVTIAEGCALCAMTLATKDTKPWGI
                     YLGYQCIRIKERSQKMLELDISELEAD"
     gene            complement(2558..4204)
                     /locus_tag="ACZO9R_03080"
     CDS             complement(2558..4204)
                     /locus_tag="ACZO9R_03080"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HIR0912090.1"
                     /translation="MRLSILSNVNLDMLSGLLKKENEVFQTDGYGEWITYALKKDERL
                     AAFAPECLFLLLDGNALLEGCQDMGSVKTVLDRSEQYIEKLAENYSASYLVVSTIDVR
                     PEHIGAGDASDLAQASGAYWDTLLTGLAERQKNIHRFELRRLIEDYGRKQFYSAKFWY
                     MGSIPYDMKALHLLAEEMRHVMARLVRVPKKVLVLDLDNTLWGGVIGEDGPEGIVLDS
                     AHEGAIYQDTQKQIKKMQQQGVLLAIASKNNSEDVQSAFRENPHMILKESDFSAIYAD
                     WNPKPVNIGKIAEELNLGLDSFVFVDDNEAEREAMRIQQPEVTVVDFPTDLTTLPAVM
                     AEVYENYFFTWHLTDEDKAKTMQYQQERERRKERESAVSYEDYLRSLQTTIRLAPVND
                     NTRERAVQLMNKTNQFNTCTLRMDELALEHYLGEEGGHLLMAEVSDKYGNSGWVSEFL
                     YHQDGDTAVIDNFLMSCRVMGRKVEDAILDAVLKKLQADGITRATAAYKKTAKNKPVE
                     ALWEQLGFTQVSGDEEQKQYERKLTSLPETEQIHTVVWDV"
     gene            complement(4206..4424)
                     /locus_tag="ACZO9R_03085"
     CDS             complement(4206..4424)
                     /locus_tag="ACZO9R_03085"
                     /inference="COORDINATES: protein motif:HMM:NF012759.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR0912091.1"
                     /translation="MEEFLSFIADILEVDKNTLTLETSQDDVETWDSLMQLRLLGEIE
                     AKYGVMIPMERISTIKKLNDFYQYIKES"
     gene            complement(4445..>4595)
                     /locus_tag="ACZO9R_03090"
     CDS             complement(4445..>4595)
                     /locus_tag="ACZO9R_03090"
                     /inference="COORDINATES: protein motif:HMM:NF037564.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3048 C-terminal domain-containing protein"
                     /protein_id="HIR0912092.1"
                     /translation="KALYVSWKKTSMTSPTRYYDAQGNEITINTGKTYVALVPVDDWE
                     DLVIE"
BASE COUNT         1055 a         1291 c         1015 g         1234 t
ORIGIN      
        1 ctggcgcgct atcacaccgg gcattgttcc aaaaagcagc tgctcctcgg aattacagga
       61 tccacggcgc tcatcttttg cgccgacgga gcgctgaccc tgcatcaggg tgtcatctac
      121 agcacttttt cgcgggattg ttcgatcttt attattttta ccgctgtttt gctgctgttg
      181 ctcttccgtg agttctcgct gcagagccgg atcatcaacc gcgcggcgac caatgtgctc
      241 gccatctacg tgctggatca ggtcatccgc agcctgctgg gacgctatct ggatctgaat
      301 gcattttccg gccagtggta tctgttcttt gttgtctgcg gctatgtgct cctcgtcatg
      361 ctgcttgccg cagtctgcaa tgagctgcgc aagctgacga tcggaagaat cgagccgtgg
      421 ctggccggca tcatcgccgc ctttctgcag gcttgcggtg cgctccttat tcgtacgggt
      481 cagcggattg ttcactggtt tatttcgatg gactgatccg cacaacaaat tatttttgct
      541 ttaatatgag gtttttatga aaaaacgatt cttatatctg cgctattact taaaacatct
      601 gcatcataag ttccgcgcca agcgcggtgg catgtacagc atcatgcaca caaagtttct
      661 cgggaaaatg ccgccttccg acgaggaggc caaccatctg atcaaggaga tgattacaag
      721 cggcaagccg ttcgctctgt gccgtctcgg aagcgccgag tttaccctga tgcagctgtt
      781 tgacgaatac cagctgtttc accacgacag actccatcag agcaacatgt acacgctgtt
      841 ccacaaggac atggacgaga tcgcgcggtg ggtcaatctc accaaagagg actgcaggga
      901 cgtcgatatc atggcatact ttgaggatca tccgatggaa gaatatctga tccgctccta
      961 ctgccagaat tcccgcctga tccgtctcga acagatcgag acgattctct atgatgtacc
     1021 gtggacgatg gcgttagaag gaaaaaaagt gctcgtcatc agtcctttcg tggataccat
     1081 ccgggaacag tacccgaaga tcgacaagat ctatgagggc aggaaaatgc ttccttcttt
     1141 tgagcttaag acattaaaat ccgtatggtt cagcggagac agggaggacg atgacttcga
     1201 cacctggttt gacgctctcc agtatctcta cgacgaggcg atgaagattg actttgacat
     1261 tgccctgctt agctgcagca ccttcggatt cgatcttgcc tgcatgttca agcgcgccgg
     1321 caaacaggcg attcagtacg gcggtgccct tcaggttctg ttcggcatca aaggcgcccg
     1381 ctgggacaac tacccgccct acagcaagta ttataatgaa tactgggtgc gcgcaccgaa
     1441 gaccgaagcg ccgagcaaac gctttgcaaa caaactggat aacggctgct actggtagac
     1501 ggctgtattt gattttaaag aataagaggg gacatcatga atgtagatca cattggatat
     1561 gcagtcaaaa atattgacgc atccagacag gcttttgaag agctcggtta tgtttttgat
     1621 ccggtcgtaa acgatacgga tcgcaatatt tttatttcgt tcggtcgtct gggtgattac
     1681 cgcatcgagc tggtatcgcc gctcgatccc gcacttcctt cgccggtcga cggcacgtta
     1741 aagaagagtg ggaacacacc gtatcacatc tgctatacaa gcagccgttt tgaggatgac
     1801 atcaaggagt tgcagcaaaa gcgttttctt ctcacaatcc cgcccgctcc cgcatgtgct
     1861 ttcgacggca ggcgcgttgc tttcctgtat catctccaaa tcggattact ggaactggtg
     1921 gaagcctagt cggcttccag ttccgatata tccagttcca gcatcttctg gcttcgctcc
     1981 ttgatgcgga tgcactggta tcccagatag attccccacg gcttcgtatc cttcgtcgcc
     2041 aacgtcatcg cacagagtgc acatccctcc gcaatcgtaa cacccggcag aatgatgcag
     2101 ccagctccga tgttggagta ctttttaatc acaaccggtt cctcaatcac attcgtgaac
     2161 ttctccggaa ccgtcggatt cgtcataaaa tctcccgagt aatcgtccga tgcggcataa
     2221 actgcacagt gcgatgaaat cccggtataa tcctcaaatg taatgcctgc cgttccgcca
     2281 aacaatgtcg tggtaaccgc cacatggata taattcccga atgtaatctt accgctgagc
     2341 acgcagaaat catccactct cacatgattg ccgatccaga tattggaggc accgtaaatg
     2401 cttgccttac ggctgatcat cacatcatgt cccacatggg caaatcccat ctgttgtaat
     2461 tcttcatccg aataaaaact tgttgccatc tctctctcca ttctgccgcg cgaaacatct
     2521 acgcgctgtt ttttattctg atcgctgcga cactatctta cacatcccag accacagtat
     2581 gaatctgctc tgtctcagga agcgaggtaa gctttcgctc gtactgcttc tgctcctcat
     2641 ccccggaaac ctgtgtaaat cccagttgct cccacaacgc ctcaaccggc ttgttcttcg
     2701 ccgtcttttt gtatgccgcc gtcgccctgg tgattccatc tgcctgaagc ttcttaagta
     2761 cggcgtccag gatcgcatct tcgactttgc gtcccatcac ccggcagctc ataagaaaat
     2821 tgtcgatcac tgccgtatcg ccgtcctgat gatacaaaaa ttcactcacc catccgctgt
     2881 ttccatactt gtcggagact tccgccatca gcagatgacc tccctcctcg ccaaggtaat
     2941 gctccagtgc cagttcatcc atccgcagtg tacaggtatt aaactgattc gtcttgttca
     3001 tcagctgcac ggcgcgctcc ctggtattgt cgtttaccgg tgccagccgg atcgtcgtct
     3061 gcagcgaccg aaggtaatct tcataggata ccgcgctctc acgttctttt ctccgctcgc
     3121 gttcctgctg atactgcatg gtctttgcct tgtcttcgtc tgtcagatgc catgtgaaga
     3181 aatagttctc atagacttct gccatcaccg ccggcagcgt cgtaaggtct gtcgggaaat
     3241 ccacgactgt cacttccggc tgctggatcc gcatcgcttc gcgctccgcc tcattgtcgt
     3301 ccacaaagac aaacgagtcc agaccaaggt tcaattcctc cgctattttc ccaatattga
     3361 cgggctttgg attccagtct gcatagatcg cagaaaaatc tgattccttc aggatcatat
     3421 gtggattctc acgaaatgca ctctgcacat cctcgctgtt attcttggac gcgatcgcca
     3481 gaagcactcc ctgctgctgc atctttttga tctgtttctg ggtatcctga tagatggctc
     3541 cctcgtgtgc actgtcgagc acaattcctt ccggtccatc ctcgccgatc acaccacccc
     3601 agagggtatt atccagatcg agaaccagca ctttcttggg aacacgcacc agcctcgcca
     3661 tcacatgccg catctcctcc gcgagcagat gaagcgcttt catatcgtaa ggaatggaac
     3721 ccatatacca gaattttgcg gaataaaact gttttcttcc ataatcttcg atcagacgcc
     3781 gcagttcaaa acggtgaata tttttctgcc gctccgccag cccggtcaga agcgtatccc
     3841 aataggcgcc ggatgcctgt gccagatcac ttgcatctcc cgcaccgata tgttccggcc
     3901 ggacatcaat ggtcgatacg acaagatagc tggcagagta attctccgcc agcttctcta
     3961 tatactgctc cgagcggtct aagaccgtct tcacacttcc catatcttgg cacccttcta
     4021 acagggcatt tccatccagc agtaaaaaca gacattccgg cgcaaacgcc gccagacgct
     4081 cgtccttttt aagtgcatac gtgatccact ctccataccc gtctgtctga aatacttcgt
     4141 tctctttttt taaaagtccg gacagcatgt ccagattcac gttggacaaa attgatagcc
     4201 gcattttatg attcctttat atattgataa aaatcgttca gcttcttgat cgtagagatc
     4261 cgctccatcg gtatcataac accgtatttt gcctcgatct caccgagcag acgaagctgc
     4321 atgagggaat cccatgtctc cacatcatcc tgggatgtct ccagtgtcag tgtattctta
     4381 tctacttcca atatgtccgc tataaaactt aaaaattctt ccatctttct atcctttacc
     4441 cgacttattc aatcaccaga tcttcccagt catcgacagg tactaatgca acgtaggttt
     4501 ttccggtatt gatcgtaatc tcattcccct gtgcgtcata ataacgtgtc ggagacgtca
     4561 tgctggtctt cttccatgat acatagagcg ccttg
//
LOCUS       DBMDQJ010000101         4594 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7164_length_4594_cov_1.627198, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000101 DBMDQJ010000000
VERSION     DBMDQJ010000101.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4594)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4594)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4594
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7164_length_4594_cov_1.627198"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="ACZO9R_03095"
     CDS             complement(<1..790)
                     /locus_tag="ACZO9R_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912093.1"
                     /translation="MEKKRRLSSLKKKIIVFVILCWGVPIAVFFAYTTSSYRKGIIEK
                     TEGLMEDQLENMASFASIRIEDAITLCQRPSYEKTWENAWKKYLRGDYSRNDYLQDVY
                     TSLRGKFYLDERFNMYAYYCYGTEDPESFSSRTGISLKSYVDEIQPYLKDVIDSDSAY
                     ACVRVVDGRIFIVRNLYTTMDYTRYGTLVVELNRSKVFQDVDTGLLNDMVVCFGSRDA
                     RVDFVKNEEKSDKEQLLEQLLQKYDGVSNHTFSKAEDATYRAYLY"
     gene            complement(1257..2669)
                     /locus_tag="ACZO9R_03100"
     CDS             complement(1257..2669)
                     /locus_tag="ACZO9R_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002565779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HIR0912094.1"
                     /translation="MIKFGTGGWRAVIGDDFTKANIQLLAQGLVNKMKSEGVTEQGIV
                     MGYDRRFLSKEAMQWAAEVFAAAQIKAYLINKSSPTPLVMFYVMKHDFPYGMMITASH
                     NPAIYNGIKVFTAGGRDADEIQTKDLEDYIADIASDGVCAVAYEDGKAAGYIEEIYPL
                     NEYIDNIIASVDMKAIRDADLKVALDPMYGVSETSLKTILLTARCEVATIHDRHDTLF
                     GGKLPAPSAATLHSLQNYVVEKQCDIGIATDGDADRIGVIDDKGNFLHPNDILVLLYY
                     YLVKFKGWHGPVVRNIATTHMLDKVAEQFGEKCYEVPVGFKYVSAKMQETGAIIGGES
                     SGGLTVHGHINGKDGIYAAALLIEMLAVTGKKLSQIMDDIHAECGEIHMEERDYKFTL
                     EKKLKMQEVLMEQKQLPDLPEEIDHVSYLDGSKVYFKNGGWVIARFSGTEPLLRIFCE
                     MPDAVEAADICTRYEQFLDL"
     gene            complement(2696..>4594)
                     /locus_tag="ACZO9R_03105"
     CDS             complement(2696..>4594)
                     /locus_tag="ACZO9R_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR0912095.1"
                     /translation="FQMSLYCAGSSYDENIIEYDLFYEEFGYQYFASNVTPDGFDCLR
                     DSFLGAYRTEDNPIAVERGTCSGSHELGNNHCGSLQKDLVLAPGEEVRLIFMLGEGSR
                     EAGKKIREKYSDMANVDAAYAQLKDYWENKFAQLQIKTPNEGMNTLINTWNLYQAEVN
                     IMFSRFASFIEVGGRTGLGYRDTAQDAMTIPHSNPEKCRQRIVELLRGLTTKGYGLHL
                     FSPEWFDPDAKKEKKKPFKSPTVIPTVNAQDIVHGLEDACSDDALWLVPSIIEYIKET
                     GDAGFADEAVTYADGGEGTVYEHMKRILDFSAEQVGATGICKGLRADWNDCLNLGGGE
                     SAMVSFLHYWAISHFLQLAEYLGRTEDVEKYQAMRERVGNVCNRELWDKEWFIRGITK
                     NGKKIGTSEDAEGKVHLESNAWAVLSGAADVEKGKLAMDSVDKYLFTPYGILLNAPSY
                     TVPDDDIGFVTRVYPGLKENGSIFSHPNPWAWAAECVLGRGDRAMKFYNALCPYYQNN
                     MIEIRQSEPYSYCQFVVGRDHTAFGRARHPFMTGSGGWAYFSATRYMLGIRPDFEHLT
                     IDPCIPADWREFSAVRRWRGAEYDIHVENPDGVMKGVQELYVDGEKVERIPVMAQGSR
                     HDVRVVMG"
BASE COUNT         1039 a         1353 c         1010 g         1192 t
ORIGIN      
        1 gatacagata cgcgcggtag gtagcgtcct ccgcctttga gaatgtgtgg ttgctcaccc
       61 cgtcatactt ttgcagaagc tgctctaaaa gctgttcctt gtcactcttc tcctcgtttt
      121 tcacaaagtc aacccgcgca tcgcggcttc cgaagcagac caccatatca ttcaaaagac
      181 cggtatccac atcctggaac accttactgc ggtttaactc caccaccagc gtgccgtacc
      241 gtgtataatc catcgtcgtg tacagattgc ggacgataaa aatacgcccg tcgaccaccc
      301 gcacgcaggc atacgccgaa tccgaatcta tcacatcttt gaggtaaggc tgtatctcat
      361 ccacataact ctttaacgag atgccggtgc gcgaagaaaa gctctccgga tcttcggttc
      421 cgtaacagta gtaggcatac atattaaaac gctcgtccag atagaacttt ccgcgcagac
      481 tggtatagac atcctgaaga tagtcgtttc tggaatagtc tccccggagg tattttttcc
      541 atgcattctc ccaggtcttc tcataggaag gtctctggca gagcgtgatg gcatcctcga
      601 tgcggatcga tgcaaaggaa gccatattct ctaactgatc ctccatcaga ccttccgtct
      661 tctcaatgat tcccttccgg taggagctgg tggtatatgc aaaaaatacc gcgatcggga
      721 caccccagca caggatgaca aatactataa ttttcttctt caatgacgac agtcgtctct
      781 ttttctccat aaaaatctcc attcctgcat gcgaaaaaat gtattttcac acggctgcct
      841 ccccgttttc tctctccatt atagcagaac accgccgcgc ggactagaca gcatataccc
      901 tgtctttgcc acgctttgac aatccaccgg gggaatgtcc gacaagcaca gacaatgagc
      961 ggactttcta gtgcctctaa tcgaattgct tcacagacaa tataaaaccg cgcgaaccga
     1021 aactcgttgc catctgctat tgaaaacatg gcagatggca attcagacat cggcttcgcg
     1081 cggttatgtt ttgttcagca attcgataag aggcacacga aagtcctaaa attgtctgca
     1141 gcttgcggac atctccggcg gagtgctcaa agcttcaaaa gacagtgatc atcgctaacg
     1201 gaaaacgcgg caggaataga aattatccgg tccgctccgt gtggcgtttt tctactctac
     1261 agatcaagga actgctcgta tctggtgcag atgtccgccg cctcgacggc atccggcatc
     1321 tcgcagaaaa tgcgcagcag cggctccgtg cctgagaagc gggcgatgac ccagcctcca
     1381 tttttgaagt agaccttgct gccatccaga taggagacat ggtcaatctc ttccggcaaa
     1441 tccggcagct gcttctgctc cattaaaact tcctgcatct taagcttttt ctccagtgtg
     1501 aacttataat cacgctcttc catatggatc tccccacatt ctgcgtgaat atcatccatg
     1561 atctgcgaaa gctttttccc ggtcactgcc agcatctcga tcagaagcgc cgccgcatag
     1621 atgccgtctt ttccgttgat gtgcccgtgt acggtcagac cgccggaaga ctcgcctccg
     1681 atgatggctc ctgtctcctg catcttagcg gagacatact taaaaccaac cggcacttca
     1741 tagcattttt ctccgaactg ctctgccact ttatccagca tatgggtggt cgctatattg
     1801 cgcaccacag gtccatgcca gcccttaaac tttacaagat agtaatacag cagcaccaga
     1861 atatcgttcg gatgcaggaa attgcccttg tcgtcgatca cgccgatacg gtccgcatct
     1921 ccgtctgtag cgataccgat gtcacactgt ttttccacca cataattctg cagtgagtgg
     1981 agcgttgcag cggaaggcgc cggaagtttt ccgccgaaca gtgtatcatg tctgtcatga
     2041 attgtcgcca cctcgcagcg ggcagttaaa agaatcgtct ttaacgatgt ctcactcacg
     2101 ccgtacatcg gatccagtgc caccttaaga tcagcgtcgc ggatagcttt catatccacc
     2161 gatgcaatga tattgtctat gtactcattg agcggataga tctcctcgat atagcccgcc
     2221 gcttttccat cctcatatgc aaccgcgcag acgccgtccg atgcgatgtc ggcaatgtag
     2281 tcctccagat ccttcgtctg gatctcgtcg gcatcacggc cgcccgcagt aaacaccttg
     2341 attccgttat agattgccgg gttatggctt gcggtaatca tcattccgta cggaaaatca
     2401 tgcttcatca cgtagaacat gacaagcggt gtcggcgatg atttgttgat caggtatgcc
     2461 ttgatctgtg ccgctgcaaa tacctctgcc gcccactgca tggcttcttt ggagagaaat
     2521 ctgcggtcat atcccataac gatgccctgt tcggtcacac cctcgctctt cattttgttg
     2581 accagcccct gcgccagaag ctgtatattt gcctttgtaa aatcgtctcc aatcacggct
     2641 ctccagccgc cggttccaaa tttgatcata tcaatgtccc ctttcttttc ctgtgctatc
     2701 ccatcacaac tctgacgtca tgtctgcttc cctgtgccat cactgggata cgttcgacct
     2761 tctctccgtc cacatacaat tcctgcacgc ccttcatcac gccgtccggg ttttccacgt
     2821 gaatatcgta ctctgcccca cgccagcgtc tgacggcaga aaactctctc cagtctgccg
     2881 ggatacacgg gtcaatggtc aggtgttcaa aatccggccg gatgccaagc atatatctgg
     2941 ttgcggaaaa ataagcccag ccgccgcttc cggtcataaa cggatgacgt gcccgtccga
     3001 atgctgtgtg gtctcttccg accacaaact ggcagtacga ataaggttct gactgccgga
     3061 tctcaatcat gttgttctga taatacgggc acagtgcatt gtagaacttc attgcgcggt
     3121 cgccgcgtcc gagcacgcac tccgctgccc atgcccacgg attcggatgg gagaaaatgg
     3181 agccgttctc cttcagtccc ggataaacgc gcgtcacaaa tccgatatcg tcatccggca
     3241 cggtgtacga tggagcattg agcagaattc catacggcgt aaacagatac ttatccacgg
     3301 aatccatcgc aagctttcct ttttccacat cggctgcacc ggaaagcact gcccatgcat
     3361 tggactccag atgtaccttt ccttccgcat cctccgatgt tccgatcttc tttccgttct
     3421 ttgtgatacc gcggatgaac cattccttat cccacagctc cctgttgcag acatttccta
     3481 cccgctcacg catcgcctga tatttttcca catcctccgt gcgtccgaga tattctgcca
     3541 gctgcaggaa gtggctgatc gcccagtaat gcaggaaaga caccatcgca ctctcaccgc
     3601 cgcctaagtt caggcagtcg ttccagtctg cgcggagtcc cttgcagatt ccggttgcgc
     3661 cgacctgttc cgcggaaaag tccaaaatcc gtttcatatg ttcatatacc gttccctctc
     3721 ctccgtcggc gtaggtcacg gcctcgtctg caaatccggc atctcccgtc tccttgatat
     3781 attcgataat ggacggcacc agccagagcg catcatccga gcaggcatcc tccagcccgt
     3841 gcacgatgtc ctgcgcattc actgtcggga tgaccgtcgg agacttaaac ggctttttct
     3901 tttccttttt tgcatccggg tcaaaccatt ccggcgagaa cagatgcagt ccgtagcctt
     3961 tggtcgtgag tccgcgcagc agttccacga tacgctgtct gcatttctcc ggattggagt
     4021 gcggtatcgt cattgcatcc tgtgccgtat cccggtaccc cagacccgtg cggccaccta
     4081 cctcgataaa cgatgcaaaa cgcgaaaaca tgatatttac ttccgcctga tagaggttcc
     4141 aggtattgat gagcgtattc attccctcat tcggcgtctt gatctgcagc tgtgcaaatt
     4201 tattttccca gtagtccttt aactgtgcat acgccgcatc cacatttgcc atgtcagaat
     4261 atttttcccg gatctttttt cccgcttcgc ggctgccctc tccgagcatg aagatcagcc
     4321 gtacttcctc tcccggcgcc agcacaagat ccttctgtaa gcttccgcag tggttgtttc
     4381 caagctcgtg ggaaccgctg catgttccgc gctccaccgc aatcgggttg tcttccgtcc
     4441 ggtatgctcc cagaaagctg tcacgcagac agtcaaatcc gtccggcgtc acattcgacg
     4501 cgaaatactg atagccgaat tcctcataga acagatcata ctcgatgata ttttcatcgt
     4561 aggatgatcc tgcacagtag aggctcatct ggaa
//
LOCUS       DBMDQJ010000102         4550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7243_length_4550_cov_1.325916, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000102 DBMDQJ010000000
VERSION     DBMDQJ010000102.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7243_length_4550_cov_1.325916"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="ACZO9R_03110"
     CDS             complement(<1..212)
                     /locus_tag="ACZO9R_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912096.1"
                     /translation="MKKSKAIVILIVILGALAGLAYYASIILSSTGIGEEMSIPLGLD
                     LSGGVSITYQVVDDNPSAEDMSDTIY"
     gene            complement(199..1569)
                     /gene="scfB"
                     /locus_tag="ACZO9R_03115"
     CDS             complement(199..1569)
                     /gene="scfB"
                     /locus_tag="ACZO9R_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HIR0912097.1"
                     /translation="MVHQYKNNGYDIVLDVNSGAIHVVDDVTYDVIALYEGHTREEIV
                     NSLRERYPETEVEEALDEVQMLVDNEELFTKDTYENYIMDFKKRPTVVKALCLHIAHD
                     CNLACQYCFAEEGEYHGRRALMSFEVGKKALDFLIANSGNRRNLEVDFFGGEPLMNWQ
                     VVKDLVAYGREQEKLHDKKFRFTLTTNGVLLNDEVMEFCNREMGNVVLSIDGRKEVHD
                     KMRPFRKGAGSYDLIVPKFQQFAESRHQDKYYVRGTFTHYNLDFSEDVLHLADLGFKQ
                     ISVEPVVAEPKEPYAIREEDLPKLFEEYDKLAVEMIRRHKSGEDFNFFHFMIDLEGGP
                     CVAKRLSGCGSGTEYLAVTPWGDFYPCHQFVGNEKFLLGNVDEGILNTDIRDEFKCCN
                     VYAKEKCRKCFARFYCSGGCAANAYNFSGDICGAYDIGCELQKKRIECAIMIKAAEEE
                     QKNEEE"
     gene            complement(1678..1818)
                     /gene="scfA"
                     /locus_tag="ACZO9R_03120"
     CDS             complement(1678..1818)
                     /gene="scfA"
                     /locus_tag="ACZO9R_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015524633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HIR0912098.1"
                     /translation="MKHVKTLNTRKLQNTIKKGGCGECQTSCQSACKTSCTVGNQSCE
                     NR"
     gene            complement(1935..2327)
                     /locus_tag="ACZO9R_03125"
     CDS             complement(1935..2327)
                     /locus_tag="ACZO9R_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HIR0912099.1"
                     /translation="MKLGYKIKPRDAKSNPTTAILAAVFVMFLISGLLLLLLALLLYK
                     MEPGESVIKIGIVAVYVISGLCGGFLMGKRMREQKFLWGLAAGIIYFLLLFGVSLLVK
                     GTGDLQMTRIFSTLILCGASGMAGGMLS"
     gene            complement(2381..3259)
                     /locus_tag="ACZO9R_03130"
     CDS             complement(2381..3259)
                     /locus_tag="ACZO9R_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HIR0912100.1"
                     /translation="MRYILENDTLRVEIESFGAEIKSVKRKADGREYMWCGDPKYWGR
                     TSPVLFPFVGAPKNKEYRYEGRTYPMGQHGFARDMEFALEAQEGKSIWFVLSSTEETY
                     AKYPFRFRLHIGYTLDENEVSVHWKVDNTDEKPMYFSIGAHPAFLCPINGEQDKTGYR
                     LRFGDLTDKLHHHGNTPDGMAVMVDEELELEDGEAVITPGFFDKCTYMVEGAQTGEVS
                     ILDRDGEAYVTVRFDAPLFALWSPEGKEAPFLCIEPWYGRCDAVDFEGSLAERPYENA
                     LAAGGSFDAEYAIVFA"
     gene            complement(3281..>4550)
                     /gene="asnS"
                     /locus_tag="ACZO9R_03135"
     CDS             complement(3281..>4550)
                     /gene="asnS"
                     /locus_tag="ACZO9R_03135"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR0912101.1"
                     /translation="NDGTFFEPVQVVFSNVLENFEEVEKLNVGAAIIVTGKLVATPEM
                     KQPFEIQAEKIEVEGASTPDYPLQKKKHSFEYLRTISHLRPRTNTFEAVFRVRSLIAY
                     AIHKFFQERDFVYVHTPLITGSDCEGAGEMFQVTTLDLANVPKNPDGTVDYTKDFFNK
                     PTNLTVSGQLNGETYAMAFKNIYTFGPTFRAENSNTTRHAAEFWMIEPEIAFADLEDD
                     MILAESMLKYIIRYVLENAPEEMAFFNNFIDKGLLDRLNNVVNSDFARVTYTEAIEIL
                     EKHNDKFEYKVSWGADLQTEHERYLTEEIYKRPVFVTDYPKEIKAFYMKMNEDGKTVA
                     AVDCLVPGIGEIIGGSQREDDYDKLLARINELGLREEDYEFYLDLRKYGSARHAGFGL
                     GFERCVMYLTGMSNIRDVIPFPRTVNNCEL"
BASE COUNT         1075 a         1353 c          942 g         1180 t
ORIGIN      
        1 ttgtagatcg tatcactcat atcctccgca cttggattgt catccacaac ctgataggta
       61 atggaaacac cgccggataa atccagacca agcggaatgc tcatctcttc cccgattccc
      121 gtcgaagaaa gaatgatgga tgcataataa gcaagtcccg ccagtgcacc aaggatcacg
      181 atcaaaataa caatcgcttt actcttcttc attcttttgt tcctcctctg cagcttttat
      241 cataatcgca cattcgatgc gctttttctg aagctcacat ccgatgtcat atgctccgca
      301 gatgtcgccg ctgaaattgt aggcattcgc cgcgcagccg ccactgcagt agaatcttgc
      361 gaaacacttg cggcatttct ccttcgcata gacattgcag catttaaact cgtcgcgaat
      421 gtctgtattt aaaatgccct catcgacatt tccgagaagg aacttttcat ttccgacaaa
      481 ctgatggcac ggatagaaat cgccccacgg ggtgaccgcc agatactcgg ttcccgaacc
      541 gcagccggaa agacgcttcg ccacacacgg accgccctca agatcgatca taaagtggaa
      601 gaaattaaaa tcttctccgc tcttatgacg tctgatcatc tcaactgcca gcttgtcata
      661 ctcctcgaag agcttcggca gatcctcctc ccggattgcg tacggctcct tcggctccgc
      721 cacaaccggc tccaccgaaa tctgcttgaa tccgagatct gcaagatgca gcacatcctc
      781 cgagaaatca agattataat gtgtaaaggt tcctctcaca taatacttat cctgatgtct
      841 gctctccgcg aactgctgga acttcggcac gatcagatcg tagcttcccg cacccttgcg
      901 gaacggccgc atcttatcgt gaacttcctt ccgtccgtca atacttaaaa cgacattgcc
      961 catctcgcgg ttacagaatt ccataacctc atcatttaaa agtacaccgt ttgttgtcag
     1021 tgtaaagcga aacttcttgt cgtgaagctt ctcctgctct ctgccgtatg ccacaagatc
     1081 cttgaccacc tgccagttca taagcggttc cccgccgaaa aaatccacct ccaggttgcg
     1141 tctgttgccg gaattggcaa tcagaaaatc aagtgccttc ttcccgactt caaaggacat
     1201 aagagccctt ctcccatggt actcgccctc ctcggcaaag caatactgac aggcaaggtt
     1261 gcagtcatgc gcaatgtgca ggcacagagc cttcacaacc gtcgggcgct ttttgaaatc
     1321 catgatatag ttctcgtagg tatccttcgt aaaaagctcc tcattgtcga ccagcatctg
     1381 cacctcatca agtgcctctt ccacttcagt ctccggatag cgctcccgca gagaattcac
     1441 aatctcctct cttgtatgtc cttcatacag ggcaatgaca tcgtatgtca cgtcatccac
     1501 cacatgaatc gcgccgctgt ttacatccag aacaatatca tagccgttgt tcttatactg
     1561 atgaaccact ataatatttc ctctctttct aattctgact attataacgt acaaatgacc
     1621 gcacgcttcg cgaacggtcg tctgtaggta atgctattca cttatgcccg tgaaaaatta
     1681 tctgttctca cagctctggt ttcctacggt gcaggatgtc ttacatgcag actgacatga
     1741 tgtctggcac tcgccgcatc cgcccttctt gatggtgttc tgtaactttc tggtatttaa
     1801 tgtcttaacg tgcttcataa taatctctcc tttatatctt acaataaaat tctattaacc
     1861 cgtatagtat agcacatgac cggttagatg aaaagccttt ttttgctgaa ctttcgcccc
     1921 acagagcggc tttttcagct taacattcct cccgccatgc cggatgcccc gcagaggatc
     1981 agcgttgaaa aaatgcgcgt catctgaaga tcacccgtac cctttacgag cagcgacaca
     2041 ccaaacagca gtagaaaata gatgattccc gccgccagtc cccacaaaaa cttctgctcc
     2101 cgcatccgtt ttcccatcag aaaacctccg caaagcccgg aaattacgta gaccgccaca
     2161 attccgatct taatgacgga ctctcccggc tccatcttat ataagagaag cgccagaaga
     2221 agcagcagaa gaccgcttat aaggaacatc acaaacacgg ctgcaaggat ggctgtcgtc
     2281 gggttgcttt ttgcatctct cggttttatt ttgtatccca gcttcataga tagacctgcc
     2341 tgattctttc tatcctatct atatgccggt tcgcgtgcgg ctatgcaaac acgattgcgt
     2401 actctgcgtc aaaactgcct ccggctgcaa gcgcattctc gtacgggcgc tccgccaggc
     2461 ttccctcaaa gtcaaccgca tcgcatcttc cataccacgg ctctatgcac aggaacggtg
     2521 cttcctttcc ttccggagac cacagagcaa acagcggcgc atcaaagcgt accgtcacat
     2581 aggcttcgcc gtcgcggtcc aggattgaca cctctcccgt ctgcgctccc tccaccatgt
     2641 aggtgcactt gtcaaagaat ccaggcgtaa tcacagcctc tccatcctcc agttccagct
     2701 cctcatccac catgactgcc atgccgtccg gcgtgttgcc gtgatgatgc agcttgtcgg
     2761 tgagatctcc gaaccggaga cgatagcccg tcttatcctg ttccccgttg atcgggcaca
     2821 aaaatgccgg atgtgcgccg atggaaaaat acatcggctt ctcatcggtg ttgtccacct
     2881 tccagtgaac ggagacttca ttttcgtcca gcgtatatcc gatgtgcagc cggaaccgga
     2941 acgggtattt cgcataggtc tcctctgtgg aggagagaac aaaccagatg ctctttccct
     3001 cctgcgcctc cagagcaaac tccatatcgc gcgcaaatcc atgctgtccc atcgggtagg
     3061 ttctgccctc ataccggtat tccttgttct tcggcgcgcc gacaaacggg aagagcaccg
     3121 gcgatgttct gccccagtat ttcgggtctc cgcaccacat atattccctg ccgtccgcct
     3181 tgcgtttcac agacttgatc tccgcgccga acgattcaat ctcgacccgc agcgtgtcat
     3241 tttccaaaat atatctcatg ctccacctca gtcttccctc ttacagttcg cagttgttga
     3301 ccgttctcgg gaacggaatg acgtcacgga tgttggacat accggtcaga tacatcacgc
     3361 agcgctcgaa tccgagtccg aatcccgcat gtcttgcgga accgtacttt ctgagatcga
     3421 ggtagaactc ataatcctcc tcgcgaagtc ccagctcgtt gatgcgcgcc agaagcttgt
     3481 cgtagtcgtc ctctctctgg cttccgccga tgatctctcc gattcccgga accagacagt
     3541 cgacagccgc caccgtctta ccatcctcgt tcatcttcat gtagaacgcc ttgatctcct
     3601 tcggataatc tgtcacaaat accggacgct tgtagatctc ctctgtcaga tagcgctcat
     3661 gctccgtctg aagatccgca ccccaggata ccttatactc aaacttgtcg ttgtgcttct
     3721 ctaagatctc gattgcctcc gtgtaggtca cacgcgcgaa atcggaattc acaacattgt
     3781 tcagacggtc aagaagtcct ttgtcgatga aattattgaa aaatgccatc tcctccggcg
     3841 cattctcaag cacatagcgg ataatatatt tgagcatgct ctccgcaagg atcatatcat
     3901 cctcgagatc tgcaaacgcg atctccggct cgatcatcca gaactccgcc gcatgtctgg
     3961 tcgtattgga attctccgca cggaatgtcg gtccgaacgt gtagatgttc ttaaacgcca
     4021 tcgcatatgt ctcgccgttt aactgtccgc tgacggtcag attcgtcggt ttattaaaaa
     4081 agtccttggt gtaatccacg gttccgtccg gattcttcgg cacattggct agatccagtg
     4141 tcgtcacctg gaacatctcg cctgcacctt cacagtcact tcccgtgatg agcggtgtgt
     4201 gcacatagac gaaatctctc tcctgaaaga acttgtggat cgcgtaggca atcagggaac
     4261 gcacgcggaa taccgcctca aaggtgttgg tgcgcggtct caaatgagaa atcgttctta
     4321 aatactcaaa gctgtgcttc ttcttctgca gcggataatc cggtgtggaa gctccctcca
     4381 cctcaatctt ctccgcctga atctcgaacg gctgcttcat ctccggggtt gcgacaagct
     4441 tacccgtcac gatgattgcc gcgccgacgt tcaacttctc cacttcctca aagttctcca
     4501 gcacgttgga aaataccacc tgcaccggct caaagaacgt accgtcattg
//
LOCUS       DBMDQJ010000103         4536 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7262_length_4536_cov_1.685006, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000103 DBMDQJ010000000
VERSION     DBMDQJ010000103.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7262_length_4536_cov_1.685006"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2571)
                     /locus_tag="ACZO9R_03140"
     CDS             complement(<1..2571)
                     /locus_tag="ACZO9R_03140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HelD family protein"
                     /protein_id="HIR0912102.1"
                     /translation="MDKRSTSEEQKSGYLGLTRADEERHLADVISVAQQNLERAGADI
                     RKVNEDLADLLETYDVKDKEGLALWNNATARLKENEYDLIRYEKARKKPYFGRIDFKD
                     GISQEEETCYIGRAGIAKNGSEPVVLDWRAPIASVYYESSMGPCTYTVSSEGTYEMDL
                     VRKRTYEIEQDVLKDFFDSDVVANDELLTKYLAKNKKAVLGEIIATIQKEQNVIIRRS
                     PRTNVIIQGVAGSGKTTVAMHRISYILYNYKDDFRPEDFYIVGRKRILLNYITSVLPE
                     LDVYGIRQLTMEQLFVRLLYEDWDDARYSYHSMERDDPKNSVKCDTEWFLDLKTYCEE
                     YEKESVPAEDVYLEKTGKLLIGADEIRRYLKEHPADSMESKMLMLGEIVHSRYENEIL
                     GRDITFPAEEKKRLDRKYTMYFGKGKWEGSVYAFYQDFLAAQAARGREVTPPGNSFDV
                     YDLAALAYIYKRIKETDPVREASHVVIDEAQDFGMMAYRCMDACLSDCTYTIMGDTSQ
                     NIHFQYGLNDWDELRRLILTGDYDAFGLLRKSYRNTVEISTYANEILRHGDFSIYPVE
                     PIIRHGAGVCVEPVQGERALLNRAAETIQGWQRKGYETIAVICRDEEEAERVAARLAE
                     DVPVKNGAKGETEEFGDGVMVLPVSYTKGLEFDAVLLFDPSEKKYPSDNGHVKLLYVA
                     ATRALHELTILHRGELSGILAGRAPQDKHIKEFAAEPLTKAREYEKPVRTKKEQMQQR
                     RVEGAMDMAEREYFGPRRIAAKAPAAEEKPVIRPAASPAVRPQTETVRGQQAKTVHRP
                     QTGAMHRPQAETVRGQQDMATSPAPAGKRPGPEGTNPSPYAFGTIPENTSLRVRGHAK
                     G"
     gene            complement(2692..>4536)
                     /locus_tag="ACZO9R_03145"
     CDS             complement(2692..>4536)
                     /locus_tag="ACZO9R_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR0912103.1"
                     /translation="SVISGKAYAKVTHVGNENYATKLANEVKKEKRVASELLGSMNKV
                     TRFTSMLIVPLGVILFFEAFLLRQAPMREAVVSSAAALLGMLPKGLVLLISISLATGV
                     IRLAKMKILVQNIYSLETLAHVDTLCLDKTGTITNGKLTVDAVMEISAGLSGKALPQG
                     EPEKLVQSYLAASDDNNATFQVLKEAFGGQAFYTPAHKIPFSSKRKWGCISFAGSGSV
                     FVGAPEKLMRKLPGELEQRLEEGCRVIIVGYVPMQWEDEAALPEEIQPLYGVVLEDTI
                     RENTRETLEFFHREGVDVKVISGDHVKTVSMIAKRAGLHRWKDAVDLSTLGEDIDYEQ
                     ICQQYAVFARVTPGQKKELVCALKRQGHQVAMTGDGVNDLLALREADCSIAVADGSDA
                     SRQIAQVVLLDSDFTNLPQVVMEGRKVVNNVTRTASVFFIKTIYSVLVSVICLLANIP
                     FPFIPIQITVLDAAIEGYPSFFTIWESDTRKIRGRFLKTALSNALPFALTVTAMVAVT
                     SIIAPFTVEQRQTVMYFLLIFISMAAVVKSCIPFNGLRTFICITMVIGTFGGLAILPS
                     LFEISAITAGMGKYLLAGGSLSVVCLLLLLGTRGVLGRKRLSEAEAVR"
BASE COUNT          937 a         1432 c         1036 g         1131 t
ORIGIN      
        1 tccctttgcg tgcccccgga cgcgcagact ggtattctcc gggatcgtcc cgaatgcata
       61 cggggacgga ttggttccct ccggtcccgg acgcttccct gccggcgccg gagacgtcgc
      121 catatcctgc tgcccgcgca ctgtctctgc ctgtggacgg tgcattgccc cggtctgcgg
      181 acggtgcact gtctttgcct gctgcccgcg cactgtctct gtctgcggac ggaccgccgg
      241 tgatgccgcc ggtcttatga ccggtttctc ctccgccgca ggcgcttttg ccgcaatgcg
      301 tctggggccg aaatattccc gctccgccat atccatggcg ccttccaccc tgcgctgctg
      361 catctgttcc ttttttgtcc ggacaggctt ctcatactcc ctggcttttg tgagcggttc
      421 cgccgcaaat tcctttatat gcttatcctg cggcgccctg cccgcaagaa ttccggacaa
      481 ctccccccga tggagaatcg tcagctcatg cagcgcgcgc gttgccgcaa catacaacag
      541 ctttacatga ccgttgtccg acggatattt tttctccgac gggtcaaaca gaagtaccgc
      601 gtcaaactcc agccctttcg tgtaggatac cggaagcacc atgacgccgt ccccgaactc
      661 ctccgtctct ccttttgcgc cgttctttac cggaacgtcc tctgccagcc gagccgcaac
      721 ccgctctgcc tcctcttcat cccgacagat caccgcgatg gtctcgtatc cttttctctg
      781 ccagccctgt atcgtctccg ccgcccggtt aagcagcgcc cgctctccct gcaccggttc
      841 cacacagacc ccggcgccat ggcggatgat cggttccacc ggatagatgg aaaaatcccc
      901 gtggcgcaga atctcgttcg catatgtaga gatctccacc gtgttgcggt agcttttccg
      961 cagaagccca aaagcgtcat aatcgccggt caggatgagt ctgcgcagtt catcccagtc
     1021 attcaggccg tactgaaaat ggatattctg cgaggtatcg cccatgatcg tgtaggtgca
     1081 gtccgacagg cacgcatcca tacaccggta tgccatcatc ccgaagtcct gcgcttcatc
     1141 gatcaccaca tgactcgcct ccctaaccgg gtctgtctcc ttgatccgct tgtagatata
     1201 ggccagcgcc gccagatcat agacgtcaaa ggaattcccc ggcggggtta cttccctgcc
     1261 acgggctgcc tgcgccgcca ggaaatcctg atagaaggca tagaccgatc cctcccattt
     1321 tccctttcca aagtacatcg tgtatttccg gtcgagccgc tttttctctt ctgccggaaa
     1381 cgtgatgtct cttcccagga tctcattctc atatctggaa tgaacaatct cccccagcat
     1441 cagcattttg ctctccatgg agtccgccgg atgctccttt agatagcggc ggatctcatc
     1501 cgcaccgatc agaagctttc ctgtcttttc cagataaacg tcctccgccg gcacgctctc
     1561 tttttcatat tcttcgcagt atgtttttaa atcgaggaac cactccgtat cgcacttgac
     1621 actgtttttc ggatcgtctc tctccatact gtggtacgag tagcgtgcat cgtcccagtc
     1681 ttcatataga agccggacaa acagctgctc catcgtcagc tgacggattc catacacatc
     1741 cagttccggg agaacgctcg tgatatagtt cagaaggatc cgcttccttc ccacaatgta
     1801 aaaatcctcc ggccggaaat catccttata gttatacaaa atgtaggata tgcggtgcat
     1861 tgccacggtc gtttttccgg aacccgccac gccctgaatg atgacgttcg tccgcggcga
     1921 gcggcggatg atgacattct gctccttctg gattgtcgca atgatctctc ccaataccgc
     1981 tttcttgttt tttgcaaggt attttgtcag aagttcatca ttggcaacga catccgagtc
     2041 gaagaaatcc ttcagcacat cctgttcgat ctcgtacgtc cgcttgcgca ccagatccat
     2101 ctcataggtt ccctcggagc ttaccgtata agtgcagggt cccatgctgc tctcgtaata
     2161 taccgatgca atgggcgctc tccagtccag caccaccggc tctgagccgt ttttcgcaat
     2221 tccggctctt ccgatataac aggtctcctc ctcctgggag atgccgtcct taaaatcaat
     2281 tctcccgaag tagggctttt ttctcgcctt ctcatagcgg atcagatcgt attcattttc
     2341 ctttaatctt gccgtcgcat tattccagag tgcaaggcct tctttgtctt tcacgtcgta
     2401 tgtctcgaga agatccgcca gatcttcgtt aaccttgcgt atgtccgccc cggcgcgttc
     2461 cagattctgc tgtgcaaccg agatcacgtc cgccaaatgc cgctcctcgt ctgccctggt
     2521 cagccccagg tatccgcttt tctgttcttc cgatgtgctg cgtttatcca tcctccgctt
     2581 caccccctgt ctttttacta taccaaaaaa gcagagatac cgcattacca aatgcatcaa
     2641 aggtatctct gcttttcttt ttctattaaa atataacgga aaaccggttc tctatcgcac
     2701 cgcctctgcc tctgacaatc tctttctgcc gagaactccg cgtgttccaa gcaaaagcag
     2761 cagacagacg acagataagc ttccccccgc aagaaggtat tttcccatcc cggcggtaat
     2821 cgcgctgatc tcaaacagcg acggcaggat ggcgagaccg ccaaaggttc ctatcaccat
     2881 cgtgatgcag atgaaggtgc gcaggccgtt aaacggaata cagctcttca ccacggctgc
     2941 catggaaata aagatcagca gaaaatacat gactgtctgc cgctgctcca ccgtaaacgg
     3001 tgcaatgata ctggtgactg cgaccatcgc cgtgactgtg agggcaaacg gcagcgcatt
     3061 ggataacgcc gtctttaaga atctgccgcg tatcttgcgc gtgtcggatt cccagatggt
     3121 aaagaacgac ggatacccct cgattgccgc atccagcaca gtaatctgaa tcgggataaa
     3181 cgggaacgga atgtttgcca gcaggcagat caccgaaacc agcaccgagt agatggtctt
     3241 gataaagaag acgctcgccg ttcttgtgac attgttcacg actttccgcc cttccatcac
     3301 gacctgcgga agattggtaa aatcggaatc cagcagtacc acctgcgcga tctggcggct
     3361 ggcatcgctt ccgtccgcca cggcgatgga gcagtccgcc tcccgcagcg ccagaagatc
     3421 attgactccg tctcccgtca tggctacctg gtgtccctgt cgtttcagcg cacacaccag
     3481 ttcctttttc tgtcccggcg tcactctcgc aaacaccgcg tactgctggc agatctgctc
     3541 ataatcaatg tcctctccga gcgtcgataa atccacggca tccttccacc ggtgcagtcc
     3601 tgcccgcttt gcaatcatgg agaccgtctt gacatgatct ccggaaatta ccttgacgtc
     3661 cacgccttcc cggtggaaaa attcaagcgt ctccctggtg ttctcacgga tggtatcctc
     3721 caggacaacc ccgtagagcg gctgtatctc ttccggcagc gccgcttcgt cttcccactg
     3781 catcgggaca tagcccacaa tgataacccg gcagccctct tccagcctct gttccagttc
     3841 ccccggcagc tttcgcatca gcttttccgg ggcgccgaca aaaacactgc ccgatcccgc
     3901 aaaactgata catccccatt tgcgctttga tgaaaatgga atcttatgtg ccggtgtata
     3961 aaatgcctga ccgccaaacg cctctttcag cacctggaat gtcgcattgt tgtcgtcgga
     4021 ggccgccaga tacgactgca cgagtttttc cggttcgccc tgcggcaacg cctttccaga
     4081 gagccccgcg ctgatctcca tgacggcgtc caccgtaagc tttccgtttg tgatcgttcc
     4141 tgtcttatcc agacacagcg tatccacatg cgccagtgtc tccagggagt aaatattctg
     4201 aacgaggatc ttcatttttg ccagccggat gactccggtc gccagtgaaa tcgagattaa
     4261 aagtaccagt cccttcggca gcataccaag aagcgccgcg gcggacgaca ccaccgcttc
     4321 ccgcatgggc gcctgccgca gcaggaaggc ctcgaaaaac agaatcacac ccaacggaac
     4381 aatcagcata ctggtaaacc gcgttacctt attcatagag cccagcagtt ccgatgccac
     4441 gcgtttttcc ttttttactt cgttggcaag ctttgtagca taattttcat ttccgacatg
     4501 cgtaaccttc gcatatgcct taccggaaat gacaga
//
LOCUS       DBMDQJ010000104         4532 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7269_length_4532_cov_1.640036, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000104 DBMDQJ010000000
VERSION     DBMDQJ010000104.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7269_length_4532_cov_1.640036"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="ACZO9R_03150"
     CDS             complement(<1..187)
                     /locus_tag="ACZO9R_03150"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856273.1"
                     /note="Catalyzes the formation of PRPP from ATP and ribose
                     5-phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HIR0912104.1"
                     /translation="MPNDERNLETMPDGALGLIPLESCKELGLKVDQYLVGWREKRQH
                     QHQNDPAFKGYRRDSYII"
     gene            complement(268..1248)
                     /locus_tag="ACZO9R_03155"
     CDS             complement(268..1248)
                     /locus_tag="ACZO9R_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0912105.1"
                     /translation="MPLSNSQYDEIFRKYDEKQLASQHQLERRTKDVYARIPALKEID
                     DAIATCSVEQARRLLDGDAAALATLREKLSDYRRQKEQLLQEHGLASDYLKPVYHCPD
                     CRDTGYVNGRRCHCFEQAAINLVYTQSNLKEILERENFSTFSFDYYSADDINPATGRS
                     SLETAKDAVAKCHDFIDHFDSTFSNLYLYGDTGIGKTFLSNCIAKELLDRGHSVIYFT
                     AFQLFDILSKGVFKRDEEALLSHRNIFDCDLLIIDDLGTELSNSFTTSQLFLCINERI
                     LRQKSTIISTNLGMNQLADIYSERVLSRISSNYTLLKLFGADIRILKRHR"
     gene            complement(1265..2371)
                     /locus_tag="ACZO9R_03160"
     CDS             complement(1265..2371)
                     /locus_tag="ACZO9R_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HIR0912106.1"
                     /translation="MANIKLHSDSPVSATSVSNTFIDEYMSDANGEFVKIYLYLLRLM
                     NAPQASFSISSIADKFEHTEKDVKRALAYWERMHLLQLEYDNDKNLTGIRMLDSSSRD
                     AAPSDPGTSGTQTEPAGAPAQNAPASAALAETPAPAQTVPAKKSYSADDILNFRQNES
                     VAELFFIAERYLGRTLSTTDINTLLYFYDVLGFSTELIVYLIESCVSNNHTSIRYIEK
                     VALGWADQHITTVEEAKQGSPVLGKMYYAVMNAFGITGRKLVSAEIAYISRWTNEYGF
                     DSDIILEACKRTMASLHQPNFKYTDSILSDWNKKQVHHLSDIAKLDAAHQERKKSSAS
                     AAANTASKNKFNNFTQRSYDYDELEQMLLTTNVH"
     gene            2462..2635
                     /locus_tag="ACZO9R_03165"
     CDS             2462..2635
                     /locus_tag="ACZO9R_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912107.1"
                     /translation="MRSIFSGTHPAQTIDRRNVDNVDKENESGGNRIMETKGLAQKTA
                     KAAKVQETCIRIM"
     gene            complement(2699..>3171)
                     /locus_tag="ACZO9R_03170"
     CDS             complement(2699..>3171)
                     /locus_tag="ACZO9R_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR0912108.1"
                     /translation="DAVAKGLSDFGGADRRFQYKGCVDGVTIIDDYAHHPTEIRATLT
                     AAKNYPHKRLVLCFQPHTYSRTKAFLEDFADVLSLADVVVLADIYAAREKNTYGVSSR
                     DILSKLRKRGTECYYFPSFEEIEKFLLKNCVNGDLLITMGAGDIVNVGEHLLGK"
     assembly_gap    3172..3181
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3182..4072)
                     /locus_tag="ACZO9R_03175"
     CDS             complement(<3182..4072)
                     /locus_tag="ACZO9R_03175"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR0912109.1"
                     /translation="MYKINFKEPIHIHFIGIGGISMSGLAEILSEEHFTVSGSDAKES
                     DLTRHLEHLGIKVYYGQTAANITDDIDLVVYTAAIHEDNPEFACAREKGIPMMPRAEL
                     LGQIMDNYRNSIAVAGTHGKTTTTSMISQILLAAKCDPTITVGGILKVLDGNLRVGKS
                     DFFLSEACEYTNSFLHFYPKYSIILNIEAEHLDFFKDIQDIRHSFHQFASNTREDGAI
                     IINGDIPDYTEITAGLKPQVITFGLSDSCTFHPADITFDEKACASFTAMKGQEALMKV
                     TLHVPGMHNVSNALAAIAMAD"
     gene            4337..>4532
                     /locus_tag="ACZO9R_03180"
     CDS             4337..>4532
                     /locus_tag="ACZO9R_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR0912110.1"
                     /translation="MIRKEMIAMLLAGGQGSRLGVLTAKVAKPAVAFGGKYRIIDFPL
                     SNCINSGIDTVGVLTQYQPLR"
BASE COUNT         1145 a          998 c         1199 g         1180 t
ORIGIN      
        1 agatgatata agaatctctg cgatagccct tgaacgcagg gtcgttctgg tgctggtgct
       61 gtctcttttc gcgccagccg acaaggtact ggtctacctt aagaccgagt tctttgcagc
      121 tttccagtgg gatgagaccc aatgctccgt caggcatggt ttctaagttt ctttcatcgt
      181 ttggcatgaa tggttcctcc attaaagaat agttacatag gttttatttt caatagattg
      241 ctctaatgaa atacagggat aaaaatgtta acggtgacgc tttaagatac ggatgtccgc
      301 gccgaagagc tttaataatg tataattgct ggatattctg gataaaacgc gctctgaata
      361 aatatcggca agctggttca tcccgaggtt tgtggagatg atcgtggatt tctgccgcag
      421 aatgcgttcg ttgatacata aaaataactg cgaggtcgta aaggaattgg atagttccgt
      481 gccgagatca tcaatgatca gcagatcgca gtcgaaaata ttccggtggg aaagcagtgc
      541 ctcctcgtcc cgtttgaaca cgcccttgct taatatatca aataattgga atgcggtaaa
      601 gtaaatgacg gaatgaccgc ggtctaacag ctccttggca atacagttgg acaaaaaggt
      661 cttaccgatg ccggtatctc cgtagaggta aagattggag aaggtgctgt cgaaatgatc
      721 gataaaatcg tggcattttg cgacggcatc ctttgctgtc tcaagggacg agcgtccggt
      781 tgccggattg atgtcatccg cagaatagta atcaaaggaa aacgtggaaa aattctcacg
      841 ttccagaatc tcttttaagt tggactgggt atagaccaga ttgatggctg cctgttcaaa
      901 acagtgacag cgtctgccgt tcacataacc ggtatccctg cagtccgggc agtggtacac
      961 cggcttcaga taatcagaag caagcccgtg ctcctgcaac agctgctcct tctgcctgcg
     1021 gtagtccgag agcttttcac ggagcgtggc cagagcggcc gcatctccgt ctaacagccg
     1081 gcgtgcctgc tcgacagaac aggtagcgat cgcgtcatcg atctctttaa gtgccggaat
     1141 ccgggcgtag acgtcctttg tgcggcgttc gagctggtgc tggctggcta actgtttttc
     1201 gtcatatttg cggaatatct cgtcatactg tgaattgctt agcggcatct gtttcccctt
     1261 taaatcagtg tacgttcgtg gtcaaaagca tctgctccag ttcgtcgtag tcgtaggagc
     1321 gctgcgtgaa attgttgaac ttgtttttgg atgctgtatt ggcggcagcg gaagcggaac
     1381 tttttttgcg ttcctggtgc gccgcatcca gttttgcaat gtcggacaga tggtgaacct
     1441 gctttttgtt ccagtcggaa agaatggaat ccgtgtattt gaagttcggc tgatgcagac
     1501 ttgccatggt gcgcttacat gcctcgagga tgatgtcgga gtcaaaaccg tactcgttcg
     1561 tccagcggct gatataggcg atctcggcgg aaaccagctt gcgtccggta atgccgaacg
     1621 cgttcatcac ggcgtagtac attttgccaa gaaccggaga accctgcttg gcctcctcga
     1681 cggtcgtgat atgctgatct gcccagccaa gtgcgacttt ttctatgtat cggatgctgg
     1741 tgtgattgtt gctcacacag ctctcaatca ggtatacgat cagctcggtg gaaaatccga
     1801 gcacatcata aaaatagaga agcgtattga tgtcggtggt gcttaaggtg cggccgagat
     1861 aacgctctgc gatgaagaac agctctgcga cggattcatt ctggcggaaa ttcaaaatat
     1921 cgtccgcgga atagctcttt ttggcaggaa ctgtctgcgc cggtgccggt gtttctgcca
     1981 aagcggcgga agcaggggcg ttctgtgccg gtgcgccggc tggctctgtc tgtgttccgg
     2041 aagtccccgg atcagacggg gcggcatctc tggacgaaga atccagcatg cggataccgg
     2101 tcagattctt atcattgtca tattcaagct gcagcagatg catgcgctcc caataggcaa
     2161 gggcacgctt gacatccttc tccgtgtgct cgaacttgtc cgcgatggaa gaaatggaaa
     2221 aagacgcctg cggtgcattc ataaggcgca gcaagtagag gtaaatcttg acgaattccc
     2281 cgttggcatc agacatatat tcatcgataa acgtgttgga aacactggtt gcagagactg
     2341 gactgtcact gtgtagctta atattggcca ttgaaaccga ctcctctttc tcaaacgtaa
     2401 caagcgaaat gtacggcagc cgccgacatt ccctgatgca ttatagcaca ggaaaactgg
     2461 aatgagaagt atcttttccg gaacacatcc ggcacagaca atcgaccgca gaaatgtgga
     2521 taatgtggat aaagaaaacg aatccggtgg aaaccggatt atggaaacga aaggtttggc
     2581 gcaaaagacg gcaaaggcgg caaaagtgca ggaaacgtgt ataaggatta tgtaaacaaa
     2641 aaaatccaca ttcaaaagtt gaagttttcc ccaattgaat gtggataaag tggataactt
     2701 atttgccgag aagatgttca ccaacattta caatgtctcc ggcgcccata gttatcaaca
     2761 agtctccgtt tacacaattt tttaataaaa atttttcgat ctcctcaaat gacggaaaat
     2821 aatagcattc cgtgccgcgt ttcctgagct tggagaggat atcgcgggag gagacgccgt
     2881 aggtattttt ctctctggcg gcgtagatgt cggcaaggac aaccacatca gcgagggaga
     2941 gcacatcggc aaaatcctct aaaaatgcct tggtgcgcga gtaggtgtgc ggctggaagc
     3001 agagaaccag ccgtttgtgt ggatagttct ttgcggcggt caacgttgcg cggatctccg
     3061 tcgggtgatg cgcatagtca tcgatgatgg tgacgccgtc cacgcagcct ttgtactgga
     3121 aacgccggtc ggcaccaccg aaatcggata atccctttgc gaccgcgtca gnnnnnnnnn
     3181 natctgccat cgcaatggcg gccagggcgt tggatacgtt gtgcatgccc ggaacgtgaa
     3241 gcgtgacttt catcagagct tcctgccctt tcatggcggt gaagctcgca caggctttct
     3301 cgtcaaacgt gatgtctgcc ggatggaatg tgcagctgtc actgagaccg aaggtaatta
     3361 cctgcggctt taatccggcg gtaatttcgg tataatccgg aatatcgccg tttatgatga
     3421 tcgcgccatc ctcccgggtg ttggatgcaa attggtggaa tgaatggcgg atgtcctgaa
     3481 tatctttaaa gaagtcgaga tgttctgctt cgatattcag aatgatgctg tatttcggat
     3541 aaaaatgcag gaaactgttc gtgtattcgc acgcttccga caggaagaaa tcggacttgc
     3601 ctacccgcag gttgccgtcg agcactttta aaataccgcc gaccgtgatg gtcgggtcgc
     3661 acttggctgc aagaaggatc tggctgatca tggaagtcgt ggtcgtcttg ccgtgggtgc
     3721 ctgccaccgc gatggagttg cggtaattgt ccatgatctg tccgagcagc tctgcgcgcg
     3781 gcatcatcgg aattcctttt tcgcgcgcac aggcgaattc cggattgtcc tcgtggatgg
     3841 cagcggtgta gacgacaaga tcaatatcat ccgtgatgtt tgcggctgtc tgaccgtagt
     3901 aaactttgat gccgagatgc tctaagtgtc tggtcagatc agattccttt gcgtcggatc
     3961 cggataccgt aaaatgctct tcggagagaa tctcggcaag cccgctcatg ctgatgccgc
     4021 cgatcccgat aaaatggata tgtatgggtt ctttaaaatt gattttatac attataattc
     4081 tacctgcctg tttttctttc tatgataaag ttcattataa ccttatctta ctgatttgca
     4141 aattcttttg ggataaaata ttttcgacag aaagtgaaag aggagtgtag aaaaagaaaa
     4201 gatttgacga aaatgtcaaa aaaaagaaaa atctgctggc aaatttgtga aaaatgatgt
     4261 acatgggtgg acgattatgt tataattata agtgcaaaga ggaatagact gactagaaag
     4321 acgagaggtg attgacgtga ttcgaaaaga aatgatagcc atgctattag ctggaggcca
     4381 gggcagccgc ctgggagtgc ttacagcgaa ggttgccaaa cctgcagttg cgttcggagg
     4441 aaagtaccgc attatcgatt tccctctgag taactgcatc aattccggaa ttgatacggt
     4501 cggagttctg acccagtatc agccattgcg tc
//
LOCUS       DBMDQJ010000105         4527 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7276_length_4527_cov_2.003615, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000105 DBMDQJ010000000
VERSION     DBMDQJ010000105.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4527)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7276_length_4527_cov_2.003615"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="ACZO9R_03185"
     CDS             complement(<1..571)
                     /locus_tag="ACZO9R_03185"
                     /inference="COORDINATES: protein motif:HMM:NF025620.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding domain-containing protein"
                     /protein_id="HIR0912111.1"
                     /translation="MGRHTESNNDESGNADAENAVIKCKDGSQVVLCGTGTLNIQANG
                     KNGIKSGATTENGEASLTIRDLTLNIDAPVNDAVNAETALEVEGGTLILSAGDDALHC
                     DYALNIGAEGTNGPSVTVTSCNEGLEGATVNVFSGKVSIQSADDCINAANADLTGYSY
                     ELNIFGGTITAHSDSGDGFDSNGDLTISGG"
     gene            complement(863..1933)
                     /locus_tag="ACZO9R_03190"
     CDS             complement(863..1933)
                     /locus_tag="ACZO9R_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR0912112.1"
                     /translation="MKYEIDFLGLKQKTKDADALCFRYYDEALQRYVVGVYDGGLTEY
                     GVALKEHLRKYYFNNDPNPKIDYVICSHPDQDHAVGLTEILENFEVGMLVMNRPWLYV
                     DELFPKVYDGGVTKASLEKRLRESYPYADALETLAKDKGIPIQEGFQGTDIDGHLTIL
                     SPSRDFYLQLLVESSKTPLHEDAHNLLAYIKNAIETWTNELIREDVETSAENESSIVV
                     LGDMGEERFLLTGDAGIRALGAAADYAERNIYSLQTVKVQQIPHHGGRHNVSPSILNR
                     VVGRIVPKGTAPTKTAVVSVAAGTDHPKKMVVNAYIRRGAKVYEARTSTLQHHCGDMP
                     PREGWNTVDPLKFSEEVEDWKE"
     gene            complement(1923..2444)
                     /locus_tag="ACZO9R_03195"
     CDS             complement(1923..2444)
                     /locus_tag="ACZO9R_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912113.1"
                     /translation="MEKLFEQLSVYEVLNNFLPGAFFCILFRWQTGVGYEVNDTVTEI
                     LVFYFAGMIISRFGSLVLEPLCGKLRFVEKEAYGDYIKASKRDPKIETLSMKNNMYRT
                     FAAAVILCGIAWAVQCIAEKSAVPRAVTIAGGWIALLVLLLAAYRKQNDYIRERIRAC
                     NKKEEKEEIKDEI"
     gene            complement(2542..2979)
                     /locus_tag="ACZO9R_03200"
     CDS             complement(2542..2979)
                     /locus_tag="ACZO9R_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912114.1"
                     /translation="MKKTLKKFLAFIMAVCIVGGMTTAYAATDASAYLSGYRATCTAK
                     SGCMIAVTVEVDGTGIMDDIGAAQIHVYRSADNKHFDYLRTFDSEDFPAMMDHDTRWY
                     YKTPITFLGLAGYYYYANVEVYAGKDGGSSTRTYMTASVQARN"
     gene            complement(2976..3884)
                     /locus_tag="ACZO9R_03205"
     CDS             complement(2976..3884)
                     /locus_tag="ACZO9R_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912115.1"
                     /translation="MSEAERVLRDSGQGSLKQSGGHRERELRDRWAELLLGDSSCDDV
                     DTELSDIAELLQEYDPDMTVTDTQVQESYKRFCEKNPQLMEPEKIAAPAAAESKKPLA
                     KSSRKPFERIVALAATLVLMLTLFTVQASGSNLFRTLAEWTASVLRIGTDSSGQVSMG
                     ENDLTEGEVRIYATPEEMLSDLHIKGELVPTWVPDRFSLEGCEARKTEAGVSCYIVYV
                     AGGSDLALKMWQKPLEDIYLVEKNQKDASRFVTGAVEHYIFYDGESKLEKAVWTRNDL
                     ECCIMGDVSQEEMEGIVESIYEGGFK"
     gene            complement(3836..4444)
                     /locus_tag="ACZO9R_03210"
     CDS             complement(3836..4444)
                     /locus_tag="ACZO9R_03210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR0912116.1"
                     /translation="MQDKFLSLGNARIRELYEKEYPTLFRIACASGLGQDFADDMVQE
                     TFRTLLEKRDDPKVANHENLQGWLIITLRNKIASEMQRRWRHMEQPLEELSAVSEAGY
                     EPAFRDSLPRQLTEDEKDLLCMFYEMQLSHREIGQILGIPEAASRMRLIRARNSYEKW
                     KKLEEKTEKMSLHSAALDKYKSEEVLRCPKLKECLETAGRVL"
     gene            4421..4527
                     /locus_tag="ACZO9R_03215"
                     /pseudo
     CDS             4421..4527
                     /locus_tag="ACZO9R_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289898.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          948 a         1335 c         1022 g         1222 t
ORIGIN      
        1 agccgccgga gatcgtcaga tcgccgttgg aatcaaagcc gtcaccgctg tcggagtgcg
       61 ccgtgatcgt cccgccgaag atgttcagtt cataggaata gccggtcagg tcagcgttgg
      121 cggcattgat gcagtcgtcc gcagactgga tacttacttt gccggaaaag acattgaccg
      181 tggcgccctc cagtccctcg ttgcaggagg tgacggtcac gctggggccg ttcgtcccct
      241 ccgcgccgat attcaaggca tagtcgcagt gcagcgcgtc atcgcccgcc gagaggatga
      301 gcgtgccgcc ctccacctcc agcgccgtct ccgcgttcac cgcgtcgttt accggcgcgt
      361 cgatgttcag cgtcaggtcg cggatggtca aagaggcctc gccattctcc gtggttgcgc
      421 cggacttgat gccgtttttg ccgttcgctt gaatattcag tgttcccgtg ccgcagagca
      481 cgacctgcga gccgtcctta cacttgataa cggcgttttc cgcatcggca ttgccggatt
      541 cgtcgttgtt gctctccgta tgtctaccca ttttaccgtt tgcaacggaa tgcttcttcc
      601 gttgctcttg gcaaaccgac ctgcgtttat catgcttttc tgtatctttc tctcttccat
      661 tgacgctccc tccgaatcca tggatttact ctgtagtatt gccgtcattc gccatgtaaa
      721 acacgcactt tgccgggcgg cagcgtgctg ttttcaagaa gtgaattaat tatacgccgt
      781 tccggttcaa aaggacgctc caaataacaa agcagccgct ctgacaatgc ctgcagagcg
      841 gctgctttgc cgtttcagtt atttattcct tccaatcctc tacttcttca ctgaacttca
      901 agggatcaac tgtattccat ccctcccgag gtggcatatc cccacagtga tgctgcagag
      961 tggacgttcg tgcctcgtac actttagcgc cgcgtcggat gtatgcgttg acgaccattt
     1021 tcttcgggtg atccgtcccc gccgcaacgg acacgacagc tgtttttgtg ggcgccgttc
     1081 ccttcgggac aattcgcccg actacccgat tcaggataga ggggctgaca ttatgacgtc
     1141 cgccgtggtg cgggatttgc tgcaccttaa cggtctgtaa actgtagata ttcctttctg
     1201 cgtaatcggc agctgcaccc agtgcccgaa tacctgcgtc gccggtgagc aggaatcgct
     1261 cctcgcccat atcgcccagt acgacgatgg agctttcgtt ctccgcagag gtctcaacat
     1321 cctcccgaat gagttcgttt gtccacgtct caatggcgtt ttttatgtat gccaacagat
     1381 tgtgcgcatc ctcatgcagc ggcgtctttg aggattccac cagcagctgg agatagaaat
     1441 ccctgctcgg cgacaggatc gtcaaatgcc cgtcgatgtc agtcccctga aacccctcct
     1501 gaatgggaat gcctttgtct tttgccagag tttccagtgc gtcggcatag gggtagcttt
     1561 cacgcagacg tttctccaaa gaggcttttg tgacccctcc gtcatacacc ttggggaaaa
     1621 gttcatccac atacagccac gggcggttca tgaccagcat gccgacttcg aagttctcca
     1681 ggatctcggt caaacctacg gcgtgatcct gatccggatg cgaacagatc acatagtcga
     1741 tcttagggtt cggatcattg ttgaaatagt acttgcgcag gtgttccttc agggccacac
     1801 catactcggt aaggccgccg tcatagacgc ccaccacata cctttgcagt gcctcatcgt
     1861 aatagcggaa gcagagcgca tccgcatctt ttgttttttg ctttaatccc agaaaatcaa
     1921 tttcatattt catcttttat ctcctctttt tcttctttct tgttacaggc ccggatcctc
     1981 tcccgaatgt aatcgttttg cttcctatag gccgccagta ataacacgag aagggctatc
     2041 cagccccccg ctatagtcac cgctctcggt acagcgcttt tctccgcgat gcactgtaca
     2101 gcccatgcga ttccacagag gatcactgcg gcagcaaagg tcctgtacat gttatttttc
     2161 atggaaaggg tctcgatttt tggatctcgc ttgctcgctt ttatgtagtc accgtaagct
     2221 tccttctcaa cgaacctcag cttcccgcaa agcggttcaa gaacgagcga gccaaacctg
     2281 ctgatgatca tgccggcgaa gtagaacacg agtatttcgg taacggtatc gttcacctca
     2341 tatcccacac ccgtctgcca tctgaacagg atgcagaaga atgcacccgg caggaaattg
     2401 ttcagcacct cgtacacact cagttgctca aataatttct ccacttctct ctcctctttt
     2461 tgtgcgtgac agacaaagaa cggcatgtag ttgccatgcc gttctttgaa atattgctat
     2521 gcttgttacc ctctgccagg ttcagttcct cgcctgcacg gaggctgtca tgtaggtgcg
     2581 cgtgctggat cccccatctt tcccggcgta cacttccaca ttggcgtagt agtaataccc
     2641 ggccaaaccc aggaaagtaa tgggcgtttt atagtaccat cgcgtgtcgt gatccatcat
     2701 ggccgggaag tcttcgctgt caaaggtccg cagataatca aaatgcttgt tgtcagcaga
     2761 tctgtacaca tggatctgcg ctgcaccgat gtcatccata attccagttc cgtccacttc
     2821 aacggtcacc gcgatcatgc acccgctttt ggctgtacag gtagcccggt agccagacaa
     2881 atatgcgctg gcatctgttg ccgcgtatgc agtcgtcatg ccccccacga tgcacacggc
     2941 cattatgaat gctaaaaact tcttaagtgt tttcttcatt tgaatcctcc ctcgtatatc
     3001 gactctacga ttccttccat ctcctcttgc gacacatctc ccatgatgca acactccaaa
     3061 tcgtttcgtg tccacaccgc tttctccagt ttagattctc catcgtagaa gatgtaatgc
     3121 tcgactgctc cagtgacaaa tctcgatgca tctttttgat ttttttcaac cagataaatg
     3181 tcctcgagtg gcttttgcca catctttaaa gccaaatcgc tgcccccagc gacatagacg
     3241 atatagcagc tgactcctgc ttctgttttc cttgcttcgc atccctctaa ggaaaaccga
     3301 tctggaaccc aagtcgggac caactccccc tttatgtgca ggtcagacag catctcctcc
     3361 ggtgttgcat agatccgaac ttctccttct gtcaggtcgt tctcccccat agagacctgc
     3421 ccagaggaat ccgtaccgat gcgaaggacg gatgcagtcc attcggccag tgtccggaac
     3481 agattggaac cggatgcctg taccgtaaat agcgtcagca tcaaaacaag tgtggccgcc
     3541 agagcaacaa ttctctcgaa tggcttgcgg gaagatttcg caagaggttt cttactttct
     3601 gctgcggcag gcgcagcgat tttctcaggc tccatgagct gcgggttctt ctcgcaaaac
     3661 cgcttatagc tctcttgcac ctgtgtatcc gtaaccgtca tgtccggatc gtactcctgc
     3721 agcagctctg caatgtccga caattcagta tctacatcat cgcagctgct gtcccccaga
     3781 aggagctccg cccatcggtc acgcaattct ctctcccgat gtcccccaga ttgcttcaaa
     3841 gaaccctgcc cgctgtctct aagcactctt tcagcttcgg acatctcagc acctcctcac
     3901 ttttatattt gtccagcgcc gcactatgta acgacatttt ctctgttttt tcttcaagct
     3961 tcttccattt ctcatagcta ttccttgcac ggatcagccg catacggctt gctgcctccg
     4021 ggatccccag tatttgccct atctcccggt ggctcagctg catttcatag aacatacaca
     4081 gaagatcttt ttcatcctct gttaactgac gcggcagcga gtcccggaag gcgggctcat
     4141 agcctgcttc ggatactgct gacagttcct ccaatggctg ttccatgtgg cgccacctgc
     4201 gctgcatctc tgacgcgatc ttattgcgca gcgtaatgat tagccagccc tgcaaatttt
     4261 catgattcgc caccttgggg tcatctcttt tctccagcag ggttcgaaat gtctcctgta
     4321 ccatgtcatc cgcaaaatct tgtcccagcc cagaggcaca ggcaattcga aacagtgtcg
     4381 gatattcctt ctcatacaac tcccgtattc gtgcatttcc caaactcaaa aacttatcct
     4441 gcaaaagtac gccgatgaga atgggttcca gaacaccaga ttttatgtgg acgatggcta
     4501 ttccggcacg aatttcaatc gcccagc
//
LOCUS       DBMDQJ010000106         4516 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7295_length_4516_cov_1.523216, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000106 DBMDQJ010000000
VERSION     DBMDQJ010000106.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7295_length_4516_cov_1.523216"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            25..>1414
                     /gene="pknB"
                     /locus_tag="ACZO9R_03220"
     CDS             25..>1414
                     /gene="pknB"
                     /locus_tag="ACZO9R_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR0912117.1"
                     /translation="MLTEGMYIADRYEIIGKIGAGGMSDVYKAKDLTLGRFVAIKVLK
                     PEFSEDLNFVTKFRTEAQSAAGLEHPNIVNIYDVGSEKGMHYIVMEYVEGITLKTYIE
                     KKGQLSFKEAVSIAIQVGRGIEAAHSKGIIHRDIKPQNIIISTEGKVKVTDFGIARAA
                     SSNTISSDVMGSVHYSSPEQARNGFVDGKSDIYSLGIVMYEMVTGRVPFDGDTTVAVA
                     IQHLQEEIVPPSAYAPNLPISMEKIILKCTQKNPDRRYESMTALLTDLRKALVSPNED
                     FVVMVPATNQDKTRIIGEDDLKQIKEEADSLYLKADQAVHARAEEMNRYDEDEDDEED
                     VDDDELDDEDDGDLNPKMEKAITIMGIVAAVVIVIIIIVIVVNLFGGIKSKKQSDTET
                     SRTETTQTESQNEDTVTVPDFRGMTYADAQTEAEKKGLKLENKGEVSSDDYDEGEIAN
                     QDPESGKEVEEGT"
     assembly_gap    1415..1424
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1425..2118
                     /locus_tag="ACZO9R_03225"
     CDS             <1425..2118
                     /locus_tag="ACZO9R_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR0912118.1"
                     /translation="SNGKGSVAVPNVTGDSEDDAVSALEKEGFKPNKTYAYDDSVEEG
                     KVISQTPSAGDKAKTGDTITIVISQGAEAVTVPDVKTTYKSEEQARALLTDFNVSVKT
                     AYSDTLPEGIVMDQSIDPGKQVEKGSDITITVSMGPSPSQSVTATTYSFKATINLPGD
                     TSNVSGATITLYDTSGNVLGQWDKAITDDFSISKTGLLAKEGYLVISWLDLNGNKIND
                     QTTSTYQFTPES"
     gene            2130..3008
                     /gene="rsgA"
                     /locus_tag="ACZO9R_03230"
     CDS             2130..3008
                     /gene="rsgA"
                     /locus_tag="ACZO9R_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR0912119.1"
                     /translation="MQGKIVKGISGFYYVHVAGSGIYECKAKGIFRNQKIKPLVGDNV
                     TIAVLDEEQMLGNIEEILPRENALIRPAVANIDQALVIFAAAKPKPNFNLLDRFLIMM
                     EYQKVPVTICFNKCDLLTGEELHAFSDVYEQCGYPVVYTSAREQRGIDVLLERLEGKV
                     TSVAGPSGVGKSSLINCLQPERQMETGAISRKIERGRHTTRHSEIIPIKEQTYIMDTP
                     GFSTLDIPGLEKEDLWWYYPEFEEYEPNCRFKGCSHIGEPDCGVKEAVEAGKISRLRY
                     ENYMQLYQELKEQKKY"
     gene            3028..3681
                     /gene="rpe"
                     /locus_tag="ACZO9R_03235"
     CDS             3028..3681
                     /gene="rpe"
                     /locus_tag="ACZO9R_03235"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR0912120.1"
                     /translation="MNCLSPSILSADFSRLGEQIRELDEAGAQYVHIDVMDGMFVPSI
                     SFGLPVIRSIRPCTDRIFDVHLMIEEPGRYIEDFVGAGADLITVHAESCKHLDRTIAS
                     IKERGILAGVALNPATPLQTVEYVLEKVDMVLIMTVNPGFGGQKFIPYTLQKVRDLKK
                     MIDARGLKTDIEVDGGVNLSNVEEIMSAGANIIVAGSAVFNGDVAENTQMFLDILNS"
     gene            3711..>4516
                     /locus_tag="ACZO9R_03240"
     CDS             3711..>4516
                     /locus_tag="ACZO9R_03240"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HIR0912121.1"
                     /translation="MKHFVIISGGTIDDSFADRWFEEEKPDAVIAADAGMEYLYRSHR
                     VPDVIVGDFDSVGTEALAYFKTLPGVAIQKLIPEKDDTDTESAIWLAIRRGAERITLL
                     GATGTRLDHVLGNIELLGIGLKAGIPIQICDSHNRIRMIDRGMTLARKEQFGTYVSLI
                     PYTECVEHLTLKGFKYPLTDACLRGFCSLGVSNEIVADTAEISFERISDDSLGVYDLV
                     DTDGYESLSIKGRTLRCVFSDGSMLIASENGRRLGVMTLPSGSADSFGGV"
BASE COUNT         1195 a          935 c         1382 g          994 t
ORIGIN      
        1 tgtaaatcag gttggaggtt taatatgtta acagagggaa tgtatatagc ggaccgttat
       61 gaaatcatag ggaaaattgg tgccggcgga atgtctgacg tatataaggc aaaagatctg
      121 acgctcggac gttttgtggc aatcaaggtt ttaaagccgg agttttcgga ggatctgaat
      181 tttgtgacaa agttccgcac ggaggcacag tctgctgcgg gattagagca tccgaatatt
      241 gtaaatatct acgatgtcgg aagcgaaaaa ggaatgcatt atatcgtgat ggagtatgtg
      301 gaaggcatca cgttaaagac atatattgag aaaaagggtc agctttcttt taaggaggca
      361 gtcagcattg ccattcaggt gggaagaggt attgaggcgg cgcatagcaa gggtatcatc
      421 caccgggaca ttaagccgca gaatattatt atttccacag aaggaaaagt aaaagtaaca
      481 gatttcggta tcgcgcgcgc agcaagcagc aacacgatca gctccgatgt gatgggctcc
      541 gtgcactatt cttccccgga gcaggcgcgc aacggatttg tggacggcaa gagtgatatt
      601 tattctctcg gtattgtgat gtatgagatg gtgacggggc gtgtgccgtt tgacggggat
      661 acgactgtgg cggttgctat ccagcatctg caggaggaga tcgttccacc gagtgcctac
      721 gcgccgaatc ttccgatcag tatggagaag atcatcttaa aatgtacgca gaagaatccg
      781 gaccgccgct atgagtccat gacggcactt ttgacggatc tgcgcaaggc gcttgtcagc
      841 ccgaatgagg attttgtcgt gatggttcct gcaaccaacc aggacaagac acgcattatc
      901 ggagaggatg acttaaagca gatcaaggag gaggcagaca gcctctatct taaggcggat
      961 caggcggtgc acgcgagagc ggaggagatg aaccgctacg atgaggatga ggacgacgaa
     1021 gaagatgtgg acgatgatga gctggatgac gaggatgacg gcgatctgaa tccgaagatg
     1081 gagaaagcga tcacgatcat gggaattgtt gcggcagtcg tgattgtgat tatcatcatt
     1141 gtgattgtgg taaatctgtt cggcggaatc aagtccaaga agcagtctga cacggagacg
     1201 tcgcgcacag agaccacgca gaccgagagc cagaacgagg atacggttac cgttccggat
     1261 ttccgcggca tgacatatgc ggatgcgcag accgaggcgg agaagaaggg attaaagctg
     1321 gagaacaagg gcgaagtatc ctctgacgac tatgatgagg gcgagatcgc caatcaggac
     1381 ccggagagcg gcaaggaagt cgaagagggc acgannnnnn nnnncagcaa cggaaaaggc
     1441 agtgtggcag tgccgaatgt aaccggagac tcggaggatg atgcagtgtc cgcactggaa
     1501 aaggaaggct ttaagccgaa caagacatat gcttacgatg acagtgtgga ggagggaaaa
     1561 gtcatttccc agacaccgtc cgcaggagat aaggcaaaaa caggtgatac gatcacgatt
     1621 gtcatcagtc agggtgcaga ggcagtgacc gtcccggatg tgaagacgac ctacaagtcc
     1681 gaggagcagg cgagagcact gctcaccgat ttcaacgtat cggtaaagac agcgtacagc
     1741 gatacgcttc cggaaggcat tgtcatggat cagagcattg atccgggcaa gcaggtggaa
     1801 aaaggcagcg acatcacgat cacggtaagc atggggccgt caccgtccca gagtgtgaca
     1861 gcgacaacct acagcttcaa ggcgacgatc aatctgccgg gcgatacgtc caacgtatca
     1921 ggcgcaacaa tcacgttgta tgatacttct ggcaatgtgc tgggacagtg ggataaggcg
     1981 atcacagatg atttttccat cagcaagacg ggactgcttg caaaagaggg ctatctggtg
     2041 atctcatggc tggatttaaa cggcaacaag atcaacgatc agaccacgag cacctaccag
     2101 tttacaccgg aatcataatg gataagaata tgcagggaaa gattgtaaaa ggaatttccg
     2161 gattctacta cgttcacgta gcgggatccg gaatttatga atgtaaggca aaaggaattt
     2221 tccgcaacca gaagatcaag ccgctggtgg gcgataacgt gacgattgcc gtcctggatg
     2281 aggagcagat gcttggaaat atcgaggaga tccttccgag agaaaatgcg ctgatccgtc
     2341 cggcggttgc caatatcgat caggcgcttg tgatttttgc ggcggcaaag ccgaagccaa
     2401 attttaatct gctggaccgt tttctgatta tgatggaata tcagaaggtg ccggttacga
     2461 tctgttttaa caagtgtgat cttctgaccg gggaggagtt gcacgcgttt tcggatgtct
     2521 acgaacagtg tggctatccg gtggtgtata cctcggcgag agagcagcgg ggaatcgatg
     2581 tgctgcttga gcggctggaa ggaaaggtga cgtcggtggc aggaccctcg ggtgtgggca
     2641 agtcatcgct cattaactgt ctgcagccgg agcgccagat ggagaccggg gcaatcagca
     2701 gaaagattga gcgaggccgc catacgaccc gccactcgga aattattccg atcaaagagc
     2761 agacttacat tatggataca ccgggatttt ccacgctgga tattccgggg ctggaaaagg
     2821 aagatctgtg gtggtattat ccggaatttg aggagtatga gccaaactgc cgttttaagg
     2881 gctgcagtca tatcggagag ccggactgcg gggtaaaaga ggcggtggaa gccgggaaaa
     2941 tcagccggct ccgctatgaa aattacatgc agctatatca ggaattaaaa gaacagaaga
     3001 aatattaaaa agaagggtgt aagagagatg aattgtttgt cgccatccat attatccgca
     3061 gatttttcaa gactgggaga gcagatccgt gaactggacg aggcaggggc acagtatgta
     3121 catattgatg tcatggacgg aatgtttgtg ccaagtattt ccttcgggct tccggtaatc
     3181 cgctccatcc gtccctgtac ggaccggatc ttcgacgtgc atctgatgat cgaggagccg
     3241 gggcgctata ttgaggattt tgtcggagcc ggagccgatc tgattaccgt gcatgcagag
     3301 agctgcaagc atctggaccg cacgattgct tccatcaaag agcgggggat tcttgcgggg
     3361 gttgcgttaa atcctgctac gccgctgcag acggtggaat atgtgcttga aaaagtggac
     3421 atggtgctga ttatgacggt gaaccctgga ttcggtgggc agaagtttat cccgtatacc
     3481 ttgcagaagg ttcgtgatct taagaaaatg attgatgcgc gcgggttgaa aacagacatt
     3541 gaggtggacg gcggggtcaa tctctctaat gtggaggaga ttatgagtgc cggcgccaac
     3601 atcatcgtgg cgggaagcgc tgtttttaac ggagatgtcg ctgaaaacac ccagatgttt
     3661 ctggatattt taaattctta aggaacggcg ggagaaaagc gggggagcgt atgaaacatt
     3721 ttgtaataat ttcaggcgga acgatcgacg attcttttgc ggacagatgg tttgaggaag
     3781 agaagccgga tgccgttatc gctgcggatg ccggaatgga atatctgtac cgcagccacc
     3841 gggtgccgga tgtgattgtc ggggatttcg attccgtggg aacggaggct cttgcttatt
     3901 ttaagacatt gcctggtgtg gcgatccaga agctgattcc ggagaaggac gacacggata
     3961 ccgagtctgc aatctggctt gcgatccggc gcggagcaga gcgcatcacg cttttgggag
     4021 cgacggggac gcggctggat catgtgctgg ggaatatcga actgcttgga attgggctaa
     4081 aggcggggat tcccatccag atctgcgatt cccataaccg catccgtatg attgatcgtg
     4141 gaatgacgct tgcgagaaag gaacagtttg gaacgtatgt gtcgctcatt ccctacacgg
     4201 aatgtgtgga gcatctgacg ctgaagggat ttaaatatcc cctcacggac gcctgcctgc
     4261 gtggattctg ttcgcttggc gtgagcaacg agatcgtggc agatacggca gagatctcgt
     4321 ttgagaggat ttccgacgat tcgctcggcg tgtatgacct tgtcgacaca gacggttatg
     4381 aatcgctttc gataaagggc aggacgctca ggtgcgtctt ctctgacggg agtatgctga
     4441 tagcgtcgga gaacggcagg cgtctcggtg tgatgacgct cccgtccggg tccgccgaca
     4501 gcttcggcgg cgtgat
//
LOCUS       DBMDQJ010000107         4510 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7308_length_4510_cov_1.529825, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000107 DBMDQJ010000000
VERSION     DBMDQJ010000107.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7308_length_4510_cov_1.529825"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2461)
                     /gene="uvrA"
                     /locus_tag="ACZO9R_03245"
     CDS             complement(<1..2461)
                     /gene="uvrA"
                     /locus_tag="ACZO9R_03245"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR0912122.1"
                     /translation="MKKTSERKYIKIRGANEHNLKNIDVDIPRDEFVVLTGLSGSGKS
                     SLAFDTIYAEGQRRYMESLSSYARQFLGQMEKPNVEKIEGLSPAISIDQKSTNRNPRS
                     TVGTVTEIYDYFRLLYARIGVPHCPKCGKEIKKQTVDQMVDQIMELPERTKIQLLAPV
                     VRGRKGEHQKLFEQAKRSGYVRVIVDGSMYELSEEIKLDKNKKHNIEIVVDRLMVKEG
                     IEKRLADSIENVLTLANGLMTVDVIGGEPIQFSESFSCPDCGISIGEIEPRSFSFNNP
                     FGACPTCFGLGYKMEFDEDLMIPDKRLSIAEGAIQVMGWQSCTDPSSFTYAILKALTE
                     EYHFSLETPYCEYPEEIRHVLIYGTDGREVKVHYRGQRGEGVYDVAFEGLIKNVQRKY
                     RETGSEVMKQEYEQFMRITPCETCKGQRLKAESLAVTVADKNIYEMTSMSVKNLNAFL
                     AEMKLGEQQHRIGDQILKEIRARVGFLNEVGQRIRLATQIGSGLVGVAYILDEPSIGL
                     HQRDNDKLLGALKNLKDLGNTLIVVEHDEDTMLAADYIVDIGPGAGSHGGEVVACGTA
                     QEIMKNPKSITGAYLSGRIKIPVPDERRKPTGFLTIKGARENNLKNIDVNIPLGIMTC
                     ITGVSGSGKSSLTNEILYKRLARDLNRARCIPGEHDEILGMEQLDKVIDIDQSPIGRT
                     PRSNPATYTGVFDMIRDLFAATPDAKAKGYKKGRFSFNVKGGRCEACGGDGILKIEMH
                     FLPDVYVPCEVCEGKRYNRETLEVKYKGKSIYDVLDMTVEEALEFFKNVPSIHRKIQT
                     LYDVGLSYVKLGQPSTELSGGE"
     gene            complement(2475..4457)
                     /gene="uvrB"
                     /locus_tag="ACZO9R_03250"
     CDS             complement(2475..4457)
                     /gene="uvrB"
                     /locus_tag="ACZO9R_03250"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR0912123.1"
                     /translation="MDHFKLHSKYKPTGDQPQAIEELVRGFREGNQFETLLGVTGSGK
                     TFTMANVIEQLNRPTLIISHNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYVP
                     QTDTYIAKDSAINEEIDKLRLSATAALVERRDVIVVASVSCIYGLGSPEDYLGMMVSL
                     RPGMEKDRDEVIRALIDIQYTRNEMDFHRGTFRVRGDVLEIFPANYGDTAIRVEFFGD
                     EIDRITEIDVLTGEIKCRLEHFAVFPASHYVVPQEKILRACDNIEAELEERVKYFKGE
                     DKLLEAQRIAERTNFDIEMLKETGFCSGIENYSRHLAGLPAGATPHTLMDYFKDDYLI
                     IVDESHITIPQIRGMYAGDQSRKSTLVDYGFRLPSAKDNRPLNFEEFEGKIDQMLFVS
                     ATPNVYEDEHELLRAEQIIRPTGLLDPEVVVRPVEGQIDDLVGEVNKEIKNHHKVLIT
                     TLTKKMAEDLTDYMRELGIRVKYLHSDIDTLERAEIIRDLRLDVFDVLVGINLLREGL
                     DIPEITLVAILDADKEGFLRSETSLIQTIGRAARNSEGHVIMYADTITDSMRVAIEET
                     ERRRKIQQEYNEAHGITPTTIQKSVRELISVSKKIAKEEMNFKKSPESMNKAELTKLI
                     ADIQKKMEKAAADLNFEAAAEYRDKMIELKGMLRDL"
BASE COUNT          942 a         1410 c          991 g         1167 t
ORIGIN      
        1 cctctcctcc cgaaagctct gtggacggct gtcccagttt tacataggaa agtcccacat
       61 cgtagagcgt ctgaatcttg cggtggatgg acggcacatt tttaaaaaac tccagcgcct
      121 cctccaccgt catatccagc acatcataaa tgctttttcc tttatacttg acctcaagcg
      181 tctcccggtt gtaccgcttt ccctcgcaga cctcacacgg cacatagaca tccggcagga
      241 aatgcatctc aatctttaag attccatccc ctccgcacgc ctcgcagcgt ccgcctttta
      301 cgttgaagct gaatcttccc tttttgtagc ccttcgcctt tgcatccggg gttgccgcaa
      361 acagatcgcg gatcatatcg aacacgcccg tgtaggttgc cgggtttgac cgcggcgttc
      421 tgccgatcgg cgactggtca atatcaatca ccttatccag ctgctccatg ccaagaatct
      481 catcatgctc tcccggaatg cagcgtgccc ggttcaggtc gcgcgccagc cgcttataga
      541 gaatctcatt ggtcagggag ctctttcccg agccggacac gccggtaatg caggtcataa
      601 tcccgagcgg tatgttcaca tcgatgtttt tcaggttgtt ctcccgtgcg cctttgatcg
      661 tcagaaatcc cgtcggtttt ctgcgctcat ccggtaccgg gatcttgatt ctgccactta
      721 agtacgctcc cgtgatcgac ttcggattct tcatgatctc ctgtgccgta ccgcaggcga
      781 ccacttctcc accgtgtgag ccggctcccg gtccgatatc cacaatatag tccgcggcaa
      841 gcatcgtatc ctcgtcgtgc tccacgacaa tcagcgtgtt gccgagatct tttaaattct
      901 tcagcgcccc cagcagctta tcgttatcgc gctgatgcag tccgatcgac ggctcgtcca
      961 gaatgtacgc aaccccgacc agcccggaac cgatctgcgt tgccagacgg atacgctgtc
     1021 ccacctcatt caggaatccg acgcgtgcgc ggatctcttt taaaatctga tcgccgatcc
     1081 gatgctgctg ctcccccagc ttcatctccg ccagaaaggc attgagattc ttcaccgaca
     1141 tggaagtcat ctcataaata tttttatccg caaccgtcac tgccagagac tctgctttca
     1201 gccgctgtcc cttacaggtc tcgcaggggg taatccgcat gaactgctca tattcctgct
     1261 tcatgacctc cgaaccggtc tcccggtact tgcgctgcac gtttttgatc agtccctcaa
     1321 aggcaacatc ataaacgcct tccccgcgct gtcctctgta atggactttc acttccctgc
     1381 cgtccgttcc gtagatcagg acatggcgga tttcctccgg gtattcgcag tacggcgtct
     1441 ccagcgaaaa atgatactcc tccgtcagcg cctttaaaat agcgtatgtg aaactggacg
     1501 gatctgtaca ggactgccag cccatcacct ggatggcgcc ttccgcgatc gagagacgtt
     1561 tatccggaat catcagatcc tcatcgaatt ccattttata accaagtccg aaacaggtcg
     1621 ggcacgcgcc gaacggattg ttgaaggaaa aacttctcgg ttcaatctcg ccgatgctga
     1681 tcccgcagtc cgggcaggaa aagctctccg agaactgaat cggttcaccg ccgatcacat
     1741 ccacggtcat gagtccgttt gccagtgtaa gcacattctc gatggaatcc gccaggcgct
     1801 tctcaattcc ttcctttacc atgagacggt ccaccacgat ctcgatattg tgcttcttat
     1861 tcttgtccag tttgatctct tccgacagtt catacatgct gccgtcgaca atcacacgca
     1921 cataaccact tcgcttcgcc tgctcaaaca gcttctgatg ctcgcccttt ctgccgcgca
     1981 caaccggcgc cagcagctgg atcttcgtgc gctccggcag ttccatgatc tgatcgacca
     2041 tctggtcgac cgtctgcttt ttgatctctt ttccacattt cgggcagtgc ggcacaccga
     2101 tccgcgcgta gagcagccgg aagtaatcgt agatctccgt caccgttccc accgtcgagc
     2161 ggggattgcg gttcgtcgac ttctgatcga tcgagattgc cggcgacaat ccctcaatct
     2221 tctcgacatt cggcttttcc atctgcccca ggaactgcct tgcataggaa gacagcgact
     2281 ccatatagcg ccgctgtcct tcggcataga ttgtatcaaa tgcgagggac gattttcccg
     2341 atcccgacaa tccggtcagc acgacaaatt cgtctctcgg aatatccaca tcgatatttt
     2401 tgaggttgtg ctcgttcgct cctctgattt taatatattt tctttcggac gttttcttca
     2461 ttgtcttatc ctttctacag gtcacgcagc atccctttga gttcgatcat cttgtcgcgg
     2521 tattccgccg ccgcctcgaa attgagatcc gccgccgctt tttccatctt tttctggata
     2581 tcggcaatca gcttcgtcag ttccgccttg ttcatcgact ccggagactt cttgaaattc
     2641 atttcttcct ttgcgatctt cttggaaaca gagatcagtt cccgcaccga cttctgaatc
     2701 gtcgtcggtg taatgccgtg tgcctcgttg tactcctgct ggatcttgcg gcgccgttcc
     2761 gtctcctcga tcgcaacacg catggaatcc gtaatggtat ccgcatacat gatcacatgt
     2821 ccctcgctgt tgcgcgccgc cctgccgatg gtctggatca gcgacgtctc agaacgcagg
     2881 aagccttcct tgtccgcatc caggattgcc accagggtaa tctccggtat gtccaatccc
     2941 tcacgcagaa ggttgatgcc caccagcaca tcgaacacat ccagacgcag atcgcggata
     3001 atctccgcgc gctctaacgt atcaatatcg gaatggagat atttgacgcg gatccccagt
     3061 tcccgcatgt aatcggtcag atcctccgcc atttttttcg tcagggtcgt aatcagcacc
     3121 ttgtggtggt tcttgatctc cttgttgacc tcacccacca gatcatcaat ctgcccctct
     3181 accgggcgca ccacaacctc cggatcgagc agtcccgtcg gccggataat ctgctccgcg
     3241 cgcaaaagtt catgctcatc ctcgtatacg ttcggcgtcg ccgaaacgaa cagcatctgg
     3301 tcgatcttgc cctcaaattc ctcaaaattc agcgggcggt tatccttcgc agacggcagc
     3361 cggaacccgt aatccaccag cgtgctcttt cgtgactgat cccccgcata cattccgcgg
     3421 atctgcggaa ttgtgatatg ggactcatcc acgataatca gataatcatc cttaaagtaa
     3481 tccatcagtg tgtgcggcgt cgcccccgcc ggaagccccg ccagatgcct ggagtagttc
     3541 tctataccgc tgcagaatcc cgtctctttt aacatctcaa tgtcaaaatt cgtgcgctcc
     3601 gcgattctct gtgcctccag cagcttgtcc tctcccttga aatacttcac gcgctcttcc
     3661 agctccgcct cgatgttatc gcacgctctt aaaatctttt cctgcggcac aacgtagtgg
     3721 gaagccggaa ataccgcaaa atgctcgagc cggcacttaa tctcgccggt cagcacatca
     3781 atctccgtta tacggtcaat ctcatccccg aaaaactcca cgcggatcgc cgtatcgcca
     3841 tagtttgccg ggaatatctc aagcacatcc ccgcggaccc ggaacgtgcc gcgatgaaaa
     3901 tccatctcat ttcgcgtgta ctgaatatcg atcagcgcac ggatgacctc gtccctgtcc
     3961 ttctccatgc ccgggcgcag cgataccatc atgccaaggt aatcctccgg gctgcccaga
     4021 ccatagatac aggaaaccga tgccacaacg atcacatctc tccgctccac aagcgcagcc
     4081 gtggcagaca ggcgcagttt atctatctcc tcattgattg ccgagtcttt tgcgatatag
     4141 gtatccgtct gcggcacata cgcctccggc tgataataat catagtatga cacaaaatac
     4201 tcgactgcat tctccgggaa aaattccttc atctccccgt agagctgcgc cgccagtgtt
     4261 ttattgtgag aaataatcag tgtcggccgg ttcagctgtt cgatgacatt cgccatcgta
     4321 aatgtcttgc ccgaacccgt cacgccgagc agcgtctcaa actggttgcc ctcacggaat
     4381 ccgcgcacca gttcttcaat cgcctgcggc tggtcacctg tcggcttgta tttggaatgt
     4441 aatttgaaat gatccacttg caaatctcct tttatgttct cataaaaaat agaaagcgcc
     4501 ctttcccggc
//
LOCUS       DBMDQJ010000108         4491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7345_length_4491_cov_1.828018, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000108 DBMDQJ010000000
VERSION     DBMDQJ010000108.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7345_length_4491_cov_1.828018"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            79..1647
                     /locus_tag="ACZO9R_03255"
     CDS             79..1647
                     /locus_tag="ACZO9R_03255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR0912124.1"
                     /translation="MDNNERNHNQSSDSFYSYNNGTNQNGTSQNNGAYSANGAYQNGS
                     AYGQNGGTYQNGGTYQNGGTYQSGTQNNSAYGQNGGTYQNGGYQQQFYQSQGAGNAAY
                     GQNPSGKKEKKRREKRERKPGGFGMKLAKCAALALVFGLVSGTVFEGVHLASGQIFGA
                     SDSTVAATGSVLTQSDSNTKIEATGASSSYVASDVSDIVEAVMPSIVSITNVSESQYQ
                     SFFGGTQTQQSTSCGSGIIVSQDADNLYIATNNHVVEGADSLTVAFSDNTTVSAEIKG
                     TDPSTDLAVIRVALSSIDSDTLNTIKVATLGNSDDLKAGQAAIAIGNALGYGQSVTTG
                     VISALNREVTVSDSNSNTNYTAELIQTSAAINPGNSGGALLNAAGEVIGINSVKYADT
                     SVEGIGYAIPISTAMPIIEDLITKEKVDEADSAYLGIVGVDVTSDVAKTYNMPTGVYV
                     AQVKENSAAEQAGIQKGDIITAFDGKDVSSMEDLSSKLQYYKAGTTVDVTIQRSSNGQ
                     YEEQTLSVTLGKKN"
     gene            complement(2231..3541)
                     /locus_tag="ACZO9R_03260"
     CDS             complement(2231..3541)
                     /locus_tag="ACZO9R_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR0912125.1"
                     /translation="MWAYESVFYQIYPLGFCGAPFENDGVLTHRIQKVNDWIPHIQKL
                     GANAVYFSPLFESDTHGYNTRDYTKIDTRLGTNADFKEVCDNLHAAGIKVVLDGVFNH
                     VGRGFWAFQDVLEKRWDSPYKDWFHISFDGNSNYNDGLWYEGWEGNYDLVKLNLHNED
                     VIAHLFSCIKGWVDEFDIDGLRLDVAYCLDHDFIRRLRAYCDTLKPDFFLVGEVVHGD
                     YNQIMNDAMLHSVTNYECYKGLYSSFNSMNMFEINHSLLRQFGPDNWTLYKGKHLLSF
                     VDNHDVTRVASILSNEAHLPLIYAMQFGMPGIPCVYYGSEWGAKARKEDGDPALRACF
                     DAPEWTTLTDFISRLAAAKKSSEALNYGDFRSVLLTNKQSIFERKSANERVLVAINAD
                     SESFTAHFDAGCGTAVDLLTGETHDFGGGSTLPPYSAAFWKMEH"
     gene            complement(3569..4384)
                     /gene="proC"
                     /locus_tag="ACZO9R_03265"
     CDS             complement(3569..4384)
                     /gene="proC"
                     /locus_tag="ACZO9R_03265"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR0912126.1"
                     /translation="MFDKIIGFIGAGNMGSAMIGGILNASLVTTGQVIASAHSKETLD
                     TIHSRFSIETTLSNETVAERSDILFLAVKPNKFDEVIPQIAPHVKSSCIIVSIAAGKT
                     IAAIEKAFGKEIKLVRAMPNTPALVGEAMSALCVNAHVTEAELSEVQAIFNSFGKAEV
                     VPESLIDAVVGVSGSSPAYVYLFIEAMADAAVADGMPRAQAYKFAAQSVYGAAKMVLE
                     TGRHPGELKDAVCSPGGTTIEAVAALERGGLRDTVITAQRACSKKSHDMSTAK"
BASE COUNT         1143 a         1122 c         1217 g         1009 t
ORIGIN      
        1 gcatcataga aagtgagaga gaaaaagaaa tactcactgg aacagaacaa agagttgtca
       61 gggaaaggag caattattat ggataacaat gaacgtaatc ataaccagtc ttccgattcc
      121 ttctattcat ataataacgg aacgaatcag aacggcacat cgcagaacaa cggagcatac
      181 agcgcaaacg gagcttacca gaatggcagc gcatacggtc agaacggagg aacctaccag
      241 aacggaggaa cttaccagaa cggaggaacc taccagagcg gcacacagaa taacagtgca
      301 tacggccaga acggaggaac ctaccagaac ggcggttatc agcaacagtt ctaccagagc
      361 cagggagccg ggaatgcggc gtacggtcag aatccttccg gcaagaagga aaagaagaga
      421 agagaaaaga gagagagaaa gccgggcggc tttggtatga agcttgcaaa atgtgcggca
      481 ctggcacttg tcttcgggct ggtatccgga acggtgttcg agggcgtaca tctcgcatcg
      541 ggtcagattt tcggagcctc ggattccacg gtggcagcca caggcagcgt gctgacacag
      601 tccgattcca acaccaagat tgaggcgacc ggcgcatcat ccagctatgt ggcatccgat
      661 gtctctgaca tcgtggaagc ggtaatgcct tctatcgtat cgatcacgaa tgtgagcgaa
      721 tcccagtatc agagcttttt cggcggaaca cagacacagc agagcacaag ctgcggttcc
      781 ggtattatcg tgagccagga tgcagacaat ctttacattg cgaccaacaa tcatgtggtg
      841 gagggtgcag acagcctcac agtcgcattc agtgacaaca caaccgtcag cgcggagatc
      901 aagggtaccg atccttccac agatcttgca gttatccgcg ttgcgttaag cagcatcgat
      961 tctgacacat taaatacaat caaagttgca acacttggca attcggatga tctgaaagcc
     1021 ggacaggcag cgatcgccat cggtaatgca ctcggctatg gtcagtctgt gacaaccggt
     1081 gtcatcagcg cattgaaccg tgaagtaacc gtatccgatt ccaacagcaa cacaaattac
     1141 accgcagaac tgatccagac cagtgcagcg atcaaccctg gtaacagtgg cggtgcactg
     1201 ctcaatgcag caggcgaggt catcggtatc aactctgtaa aatatgcaga cacaagtgtt
     1261 gagggaatcg gttatgcgat cccgatcagc acagcaatgc cgatcattga ggacctgatt
     1321 acgaaggaaa aggtagatga ggcagacagt gcatatcttg gtatcgtcgg cgtcgatgtc
     1381 acaagcgatg tggcaaaaac ctacaatatg ccgaccggtg tctatgtagc acaggtaaaa
     1441 gagaactccg ccgcagagca ggctggaatt cagaagggcg atattattac cgcatttgac
     1501 ggcaaggatg tctcctcgat ggaggatctg tcctctaaac ttcagtatta caaagcagga
     1561 acgaccgtgg atgtcaccat ccagagatct tccaacggac agtatgaaga gcagacgctc
     1621 tctgtcacac tgggaaagaa gaactgacag ggaaagggct gacacagagg cagtaagttt
     1681 atgataggag cacttgcagg gataagttcc aaatgcaaca ggcggcggta aatctgaccg
     1741 caaaatccgg catttctaac cacgccgcgc ttcgcatctg cgatgatctg tgtctttcca
     1801 agctttgaac actccgcagg atgtgtccgc aacctgcaga caattttagg attctcgtgt
     1861 gtcccttgcg gcaccgctga ataaaacata accgcgcgaa gccgatgttt gaattgccat
     1921 ctgccatatt ttcaatagca gatggcaacg agtttcggtt cgcgcggttt tatattgtct
     1981 gtgaagcggt gccgctaggg acactaggga attcgctcat tgtctgcggt ttgtcggaca
     2041 catcctgcgg agtgtcaaag cgtggcaaga gacacagaat gtgatgcaaa gtgcggcatg
     2101 gaagaaatac tcgaattttg cttcaaagat ttaccgccgc ctgttgcatt ttggaaccag
     2161 acacctgcaa gcccgttatg ataagccggc tgcctctgcg tcagtccctt tgatctgtca
     2221 gtcttctgtc tcagtgttcc atcttccaga aggctgcgga gtaaggcggg agcgtactgc
     2281 cacctccaaa gtcgtgcgtc tccccggtga gcaggtcaac agctgtgccg cagcctgcgt
     2341 cgaaatgcgc cgtgaagctt tcggaatcgg cgttgatggc gactaacaca cgctcattgg
     2401 cgcttttccg ctcaaagatg gactgcttat tcgtgagcag gacagagcgg aaatcgccgt
     2461 agtttaacgc ctcggaagac tttttcgcgg cggcaaggcg gctgatgaaa tccgtgagcg
     2521 tggtccattc cggtgcgtca aagcaggcgc gcaatgcggg atcgccgtcc tcttttcgcg
     2581 ctttggcacc ccattcactt ccgtagtaca cgcatggaat gcccggcatg ccgaactgca
     2641 tggcatagat taacggaagg tgcgcctcgt ttgagaggat gcttgccaca cgggtgacgt
     2701 catggttgtc gacgaaggag agcaggtgct ttcccttgta cagagtccag ttgtccgggc
     2761 cgaactggcg cagcagagag tgattgatct cgaacatgtt catggagtta aaactggaat
     2821 acagtccctt gtagcactca tagtttgtca cggagtgcag catggcgtcg ttcatgatct
     2881 ggttgtaatc gccgtgtacg acctcgccga ccaggaagaa atctggcttt aaggtgtcac
     2941 agtaggcgcg cagccttcgg ataaaatcat ggtcgaggca gtaagccaca tccagccgca
     3001 gtccgtcgat gtcaaactca tccacccagc ccttgatgca ggaaaagagg tgtgcgatga
     3061 catcctcgtt gtgcagattc agctttacga gatcataatt cccttcccag ccctcatacc
     3121 acagcccgtc gttataattg gaattcccgt caaaactgat atgaaaccag tccttgtagg
     3181 gagagtccca tctcttctct aaaacgtcct ggaatgccca gaaaccgcgg ccgacgtgat
     3241 tgaagacgcc gtctaagacg actttgatgc cggcggcgtg gaggttgtca cacacttcct
     3301 taaaatccgc gttggtgcca agtcttgtgt cgatcttcgt gtaatctctg gtattgtagc
     3361 cgtgggtgtc cgattcaaag agcggtgaaa aatatacggc gtttgcgcca agcttctgaa
     3421 tatgaggaat ccagtcgttt accttttgga tgcggtgggt gagaaccccg tcattttcaa
     3481 aaggtgcgcc gcagaaccca agcggataga tctgataaaa tacactttca tatgcccaca
     3541 taatgcgttg cctcctgtga ttgctttttt atttcgctgt gctcatgtcg tgtgattttt
     3601 tagagcaggc gcgctgggca gtgattacgg tgtcacgcag accgccccgt tccaatgcgg
     3661 cgacagcctc gatcgtcgtt ccgcccggag agcacacggc gtctttcagt tcgccggggt
     3721 gtctgccggt ctccaatacc attttggctg cgccgtagac ggactgggcg gcaaatttgt
     3781 aagcctgtgc ccgtggcatg ccgtcagcaa cggcggcgtc tgccatcgcc tcgataaaca
     3841 gatagacata ggcaggagag gaaccggaca cgccaacaac ggcgtcgatg aggctttccg
     3901 gcacgacttc tgcttttccg aaggagttga aaatcgcctg cacttcggaa agctcggctt
     3961 cggtaacatg tgcattgacg cacagggcgc tcattgcctc gccgacgagg gcaggtgtgt
     4021 ttggcatggc gcgaacgagc ttgatctctt tgccaaacgc tttttcgatg gcagcaatgg
     4081 tcttgccagc agcgatggag acgatgatgc agctgctctt cacatgcggg gcgatctgcg
     4141 gaatcacctc atcgaacttg ttcggcttga cggcgagaaa cagaatatcg ctgcgttcgg
     4201 cgacggtctc attggagagt gtcgtctcga tggagaaccg gctgtgaatg gtgtcaagcg
     4261 tctccttgga gtgtgcactg gcgatcacct gccctgtggt taccagggag gcgtttaaga
     4321 tgccgccgat catggcgctg cccatgttgc cggcaccgat gaaaccaatg attttgtcaa
     4381 acatatcaaa aaccaccttt ctatgtattg tatgctgata ccagaacagc cggctaaaga
     4441 tagccggctg tctttctata aaatatggta gcgtttttag aaccaaacgt c
//
LOCUS       DBMDQJ010000109         4491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7346_length_4491_cov_1.487244, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000109 DBMDQJ010000000
VERSION     DBMDQJ010000109.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7346_length_4491_cov_1.487244"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="ACZO9R_03270"
     CDS             complement(<1..1208)
                     /locus_tag="ACZO9R_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR0912127.1"
                     /translation="MDITGRKLFVKALQEEGVTTLFGYPGGTVTDLFDELYKQDGIEV
                     VLPRHEQGLIHEAEGYARSTGKVGVCLVTSGPGATNIMTGLADAHYDSIPLVCFTGQV
                     PLSLIGNDAFQEVDIVGMTRSITKYGVTVRKREDLGRIIKMAFYIASTGKPGPVLIDI
                     PKDIQMALGPAEYPSSVQIRGYKPNESVHVGQLKKAYKLLRGAKKPLILAGGGINIAH
                     ANDKLLQFAEKMQIPVVTTIMGKGAIPTGHPLYVGNSGMHGKYAANMAVSKCDVLFSI
                     GTRFNDRITGDLNEFAPKAKIVHIDVDTAAISRNVVVDVPIVSDANLALEKLLEWAES
                     KDTAAWQAQIAGWDEKNPLEMRRDHGMTPQMIFEHVNRTFGEAVYVTDVGQHQMWATQ
                     YLELDSWHQL"
     gene            complement(1343..2296)
                     /locus_tag="ACZO9R_03275"
     CDS             complement(1343..2296)
                     /locus_tag="ACZO9R_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HIR0912128.1"
                     /translation="MENFIYSVDATFPIFLVMVIGYILKQIGMLNDNFVTVANRFNFK
                     VTLPFMLFRDISGVDIRAVFDIRYVLFCALVSTACFWIIWGGVKLFLKDQSMRGAFVQ
                     ASFRSSAAVMGLAFIQNMYGSSAMGPLMIVSAVPLYNIFSVIVLTFEGAHSGEVDPKQ
                     KIKAACINIAKNPIILGILAGLIVGLLGIDFPVIVDKTVNSVAQMATPLALITIGAGF
                     EGRKALAKIRPTIVASMIKLVIQPLIFLPVAAWMGFRGEQMIAILIMLASPTTPSCYI
                     MAKNMDNDGVLTASVIVMTTLLAAFTLTGWIFILKTVGLIG"
     gene            complement(2313..2803)
                     /locus_tag="ACZO9R_03280"
                     /pseudo
     CDS             complement(2313..2803)
                     /locus_tag="ACZO9R_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742108.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
     gene            complement(2827..3675)
                     /gene="thyA"
                     /locus_tag="ACZO9R_03285"
     CDS             complement(2827..3675)
                     /gene="thyA"
                     /locus_tag="ACZO9R_03285"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HIR0912129.1"
                     /translation="MSLADKIFVDMCKDILENGTSTEGEKVRPHWEDGTSAYTIKKFG
                     VVNRYDLSKEFPAITLRKTAIRTCTEEMLWIWQRKSNNIHDLNSTVWDEWADADGSIG
                     KAYGYQLGVKHQYKEGMMDQVDRVIYDLKNNPFSRRIMTNIYVHADLHEMNLYPCAYS
                     MTFNVTHQPGDEKLTLNAILNQRSQDVLAANNWNVCQYAVLTHMLAQVCDMKVGELVH
                     VIADAHIYDRHVPIIRELIEREQHPAPKFTLNPDVKDFYRFTTKDITIEDYVTGPQIK
                     NIPIAV"
     gene            3853..4095
                     /locus_tag="ACZO9R_03290"
     CDS             3853..4095
                     /locus_tag="ACZO9R_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912130.1"
                     /translation="MSQAKVDRYKEEKKNRKKTMAKEKRLHLLAVVCGWLVVIALAGW
                     AGVSGYRIYESKKPVETIYANVDAITDYMNSLSTEE"
     gene            complement(4165..>4491)
                     /locus_tag="ACZO9R_03295"
     CDS             complement(4165..>4491)
                     /locus_tag="ACZO9R_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HIR0912131.1"
                     /translation="GESVETMRYVAGVYTSSIQFGEHAVDMQVIVDENRIQSVSMVNL
                     DETVETMYPLMEPALENIADQVVEKQTTEGITYNADYQYTSVVLLNAIENALAKAELS
                     GAETGE"
BASE COUNT         1022 a         1319 c          962 g         1188 t
ORIGIN      
        1 atcagctgat gccaggaatc cagttccaga tactgtgttg cccacatctg atgctgtccc
       61 acatccgtca catagaccgc ctctccgaac gtccggttca catgctcaaa aatcatctgc
      121 ggtgtcatgc cgtgatccct gcgcatctca agcggatttt tctcatccca cccggcaatc
      181 tgcgcctgcc atgctgcggt gtctttggac tccgcccact cgagcagctt ctctaacgct
      241 aggttggcat cggaaacaat cgggacatcc acgaccacgt ttcgggaaat cgccgccgta
      301 tccacgtcga tatgtacgat cttcgccttc ggcgcaaact cgtttaaatc gccggtaatg
      361 cggtcgttga atctggtacc gatggaaaac agcacgtcac atttgctgac tgccatattt
      421 gcggcatatt ttccgtgcat accgctgttc cccacataaa ggggatgccc ggtcgggatc
      481 gctccttttc ccatgatcgt cgtcacaacc gggatctgca ttttttccgc aaactgaagc
      541 agcttgtcgt tggcatgtgc aatgttaatg cctccgcccg ccagtatgag cggcttcttc
      601 gccccgcgca gcagcttata agcttttttc agctgtccca catgcacgct ctcattcggt
      661 ttgtaaccgc ggatctgcac actggaagga tactctgccg gcccgagagc catctggatg
      721 tccttcggga tatcgatgag caccggtccg ggcttccccg tggaggcaat gtagaacgcc
      781 atcttgatga tgcgccccag atcctctctt ttccgcaccg tcacgccgta cttggtgatg
      841 ctccgcgtca tgccgacaat atccacctcc tgaaatgcat cgtttccaat gagggataac
      901 ggcacctgtc cggtaaaaca gaccagagga atactgtcgt agtgcgcatc cgcaagcccc
      961 gtcatgatat tggtggctcc cggtccgctc gtgaccagac atactcccac ttttcccgtc
     1021 gaccgcgcat atccttccgc ctcatggatc agcccctgct catgtctggg taagacgact
     1081 tcgataccat cctgcttata tagttcatca aaaagatccg tcaccgttcc gcccggataa
     1141 ccgaacaggg tggtaacgcc ttcttcctgc aaagctttca caaataactt tctgcctgta
     1201 atatccatgc cgtcctcctt gccttctctc tgccgtccat ttcctgcaga aaggattatt
     1261 gtaaaaaact atagcccatc tttttcacaa atgcaatcct atccaaagat aaatcaagaa
     1321 tattaaaata catcaagaac ctttacccta tcaatcctac tgtcttcaaa ataaaaatcc
     1381 atccggtcag ggtaaatgcc gccagaagtg tcgtcatcac gatcacgctc gcggtgagta
     1441 caccgtcgtt gtccatgttc ttcgccatga tgtagcagct cggcgtcgtc ggcgatgcca
     1501 gcataatcag aattgcgatc atctgctccc cacggaatcc catccacgca gcgaccggca
     1561 gaaaaatgag cggctggatg accagcttga tcatggatgc tacaatcgtc ggacggatct
     1621 tcgccagtgc ttttcttccc tcaaatcccg ctccgatcgt gatgagtgcc agaggcgttg
     1681 ccatctgcgc cacgctgttc accgtcttat ccacgatcac tggaaaatcg atgccaagaa
     1741 gtcccacgat aagtccggcc aagattccca gaatgatcgg attctttgcg atgttgatgc
     1801 aggcagcctt aatcttctgc ttcggatcca cctccccgga atgtgcccct tcaaacgtca
     1861 ggacaatcac ggaaaaaatg ttgtagagcg gcactgcact cacgatcatg agcggtccca
     1921 ttgccgagct tccgtacatg ttctggatga acgccagtcc catcaccgcc gcactgctgc
     1981 ggaacgacgc ctgcacaaaa gctccccgca tgctctgatc cttcaaaaac agcttcacac
     2041 cgccccagat gatccagaag cacgccgtgc tcacgagcgc gcagaacagc acataccgga
     2101 tgtcaaagac cgcccggata tccacgccgg aaatatcgcg gaacagcata aacggcagtg
     2161 tcaccttaaa gttgaaccgg tttgccaccg tgacgaaatt gtcgtttaac atcccgatct
     2221 gttttaggat ataaccgatc accatgacta aaaagattgg aaacgttgca tccacactgt
     2281 aaataaaatt ctccatctct acgccatccc gcctatttta ccctctcgta ccggtaaaaa
     2341 taatactcca gatcaagtac gtctgctcct cgctgtccgc cgtaatcttc cactccggtc
     2401 tctcatccag attcgggaaa tacgcatctg catcgtatgc gtagtcaatt tttgtgatgt
     2461 gcgccacgtc gcactcatcc agaagctgtc tgtagatcgt ctctccgccg atcacataga
     2521 tatcctgact gtcgtatttt tttaattctt catgaagctc atccatgtca tgaacgacaa
     2581 ccgcacccgg aaccttatag gtatgatcgt gtgtcagcac gatattggtg cggttcttaa
     2641 gcggaagccc gttcggaaaa ctctccagcg tttttcttcc catgaccacc accttgccgg
     2701 tcgtcgtctc acggaaaaac ttctgatccg ccggaattct caccagtaac tcatttttat
     2761 tgccgattgc ccaattttga tccactgctg ctattaaatt cattttttac gcctttctgt
     2821 tcgtgcttac accgcgatcg ggatattttt gatctgcggt cccgtcacgt aatcctcgat
     2881 tgtgatgtcc tttgtcgtga accggtaaaa atctttgacg tccgggttga gggtaaactt
     2941 cggtgccgga tgctgctctc tctcgatcag ttccctgata atcggcacgt gccggtcata
     3001 aatatgcgca tccgcgatca catgtaccag ttccccgacc ttcatatcgc atacctgcgc
     3061 cagcatatgc gtcagcaccg catactggca gacgttccag ttgttggcgg caagcacatc
     3121 ctgcgaacgc tggtttagga tcgcgttgag cgtcagcttc tcatctcccg gctggtgcgt
     3181 cacattgaac gtcatactgt atgcgcacgg atacagattc atctcatgca gatccgcatg
     3241 cacatagata ttcgtcatga tgcgcctgct gaacggattg tttttcagat cgtagatcac
     3301 acggtcaacc tgatccatca tgccttcctt atactggtgc ttcacgccga gctggtaacc
     3361 gtatgctttt ccgatggagc cgtccgcatc cgcccactca tcccagaccg tgctgtttag
     3421 gtcatggatg ttgttggact tgcgctgcca gatccagagc atctcctccg tgcaggtcct
     3481 gatcgccgtc ttgcggagcg tgatcgcagg gaactctttt gagagatcat agcggttcac
     3541 cacaccgaat ttcttgatcg tataggcgct cgtcccatct tcccagtgcg gtcttacttt
     3601 ttccccctcc gtgctggttc cgttttccag tatatcctta cacatatcca caaaaatctt
     3661 atctgcgaga ctcatgccgt tcctcccgtt tttcttccat tctattctgc taattcttaa
     3721 ttgtactcaa ttttccgcga aatgacaagg tgcatttctg ttgcattttt cccgcaaatg
     3781 ggttatatta tttcttggaa ttttacttta ttttgcagag aataaagatt gaacacataa
     3841 aggagacaga ttatgagtca ggctaaagta gaccgctata aagaagagaa aaagaaccgc
     3901 aagaagacta tggcaaagga gaagcgcctg catctgctcg ctgttgtctg cggctggctg
     3961 gttgtcatcg cgctcgcagg ctgggctggt gtgtccggtt accgcatcta tgagagcaag
     4021 aagccggttg agaccattta cgccaatgtc gatgccatca ccgattacat gaattcctta
     4081 agcacagaag aataacgcaa aagaacccgg ggcgactttt ccgcacccgg gtatttttct
     4141 gccatcctgc cattttacgc tcttctactc tcccgtctct gcgccggaga gttccgcctt
     4201 cgccagtgca ttctcaatcg cgttcaacaa taccactgac gtatactggt aatccgcatt
     4261 gtatgtaatt ccttccgtgg tctgtttttc caccacctga tccgcaatgt tttccaatgc
     4321 cggctccatc agcggataca ttgtctccac cgtctcatcg agattgacca tggagacgga
     4381 ctgaatccgg ttttcatcca caatcacctg catatccacc gcatgctctc caaactggat
     4441 cgatgacgtg tacactcccg ccacatacct cattgtctct accgactctc c
//
LOCUS       DBMDQJ010000110         4490 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7350_length_4490_cov_1.103213, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000110 DBMDQJ010000000
VERSION     DBMDQJ010000110.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7350_length_4490_cov_1.103213"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="ACZO9R_03300"
     CDS             <1..241
                     /locus_tag="ACZO9R_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015527555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR0912132.1"
                     /translation="IDISLPKPRAAMRAARAASSNAPSGGSYTVKKGDCLWKIAKQYY
                     GNGSKWGTIYNANKSVIGGNPNLIYPGQVLAIPAA"
     gene            251..1207
                     /locus_tag="ACZO9R_03305"
     CDS             251..1207
                     /locus_tag="ACZO9R_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdQ/YqbQ family protein"
                     /protein_id="HIR0912133.1"
                     /translation="MYELLIQHDSTAYMPPVKEEVKVTTERQISPGVLEFSFVDTGIN
                     IGNGDPVRFKDADGKEVFYGFIFRMKRDRSNIVTITAYDQIRYLKNKDTLVYENKTAD
                     GVVALIGEKYGFNIGTLANTVWVIASRVEDNVSLLDMISNALDQTLQNTGDLYILHDD
                     FGKLNLSFLGDMYVPIMIDAETGQNYDYESSIDENTYNRIKLVYDNEDAGKREVYIAQ
                     DSSNINRWGILQYFDALQKGENGQAKADALLQLYNKETRTLTVKDAAGDSRVRGGSLV
                     VVQLDLGDVKLQNLMLVEKCVHKYGESKHTMDLTVSGGDFSA"
     gene            1191..1565
                     /locus_tag="ACZO9R_03310"
     CDS             1191..1565
                     /locus_tag="ACZO9R_03310"
                     /inference="COORDINATES: protein motif:HMM:NF022300.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HIR0912134.1"
                     /translation="MILVHDANDFVRAVQQVSTNANDAGYPATVMSGTVTSASPLKIK
                     IEQRFEISGSMLILPEHLKEREIKVTVKPTHTEDGGTPEHNHEYGGELTVTVHSGLSI
                     GDSVQVVRQQGGQKYLVIGKVV"
     gene            1568..1963
                     /locus_tag="ACZO9R_03315"
     CDS             1568..1963
                     /locus_tag="ACZO9R_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HIR0912135.1"
                     /translation="MIPVSNQLKNVEVVEQPSLCPRMIVESERIIGQCDDVEAIKQAI
                     YNILNTERYQYIIFSWDYGVELKDLFGKPIDYVMPEVERRITEALVQDDRIDSCDSFE
                     FEKKGRKLLVTFVAHTKFGSVPAQKEVDV"
     gene            1964..3001
                     /locus_tag="ACZO9R_03320"
     CDS             1964..3001
                     /locus_tag="ACZO9R_03320"
                     /inference="COORDINATES: protein motif:HMM:NF016736.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HIR0912136.1"
                     /translation="MYEEQTFDAIMQRMLERIPDTLDKRESSPVYMALAPAAVELASL
                     YVGFDCMLAETFGDTASREYLIRLCADRGITPKTATYAVLELETDVEVPVGTRFTGGD
                     HFYKVTASGQVTCEQPGAAGNEYLGDVIPVEYVMGLTTAKLTRVLIYGEDDEDTEVLR
                     LRYQESFNERAFAGNAKDYHDKTLGIAGVGAVKVIRAWNGPGTVKLVILDSVFGKATD
                     VLIQTVQKEFDPNKDGHGDGLAPIGHAVTVDTASEVTVNIAATITYDNGYDLNTCKTQ
                     IETAIEEYFAGLRKNWENQSKLVVRIASIDAAIMGVKGVVDVTGTTLNGGGNVELTEY
                     EIPVLGVVTYG"
     gene            2994..3647
                     /locus_tag="ACZO9R_03325"
     CDS             2994..3647
                     /locus_tag="ACZO9R_03325"
                     /inference="COORDINATES: protein motif:HMM:NF021583.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HIR0912137.1"
                     /translation="MADRYINLKELLPLYLQAYKELAAPMDAETPEFQIIEAEHNRII
                     ANRYIVTCDEEGIVRYEQLMGIQPKADDTLEDRIFRCITKWNVCLPYNYAFLDQKLRE
                     LCGAEYTLDLDIAGQTVTVKVGLAQKNQYDVVAEMLEEIVPCNLQLNLSLLYNQYQAL
                     KPYPHIILAQFTHWELRNLSIPRNLSAAVENIAAYAVDDLARFTVEEVADIGIRKKV"
     gene            4237..4490
                     /locus_tag="ACZO9R_03330"
                     /pseudo
     CDS             4237..4490
                     /locus_tag="ACZO9R_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT         1264 a          847 c         1338 g         1041 t
ORIGIN      
        1 gattgacatt tcgcttccga agccgagagc tgcaatgcgg gcagcaagag cggcatcatc
       61 caacgcgccg tccggcgggt cttacacggt gaaaaagggg gattgcctgt ggaagattgc
      121 aaagcagtat tatgggaacg gaagcaagtg gggaacgatt tacaatgcca ataagtcggt
      181 gatcggcggg aatccgaatc tgatctatcc ggggcaggta cttgccattc cggcggcgta
      241 ggagggacat atgtacgagt tattgataca gcatgatagc acggcgtata tgccgcctgt
      301 gaaagaagaa gtgaaggtta cgacggagcg gcagatcagc cccggagtgc tcgaattcag
      361 ctttgtggac actggaatca atattggaaa tggtgatccg gttcggttca aggatgcaga
      421 tggaaaagaa gtgttctatg gattcatttt tcgcatgaaa cgcgatcgca gtaacattgt
      481 gacgattacg gcatatgacc agatccggta tctgaagaat aaggatacgc tcgtatatga
      541 gaataagacg gcggatggtg tggtggcgct gatcggtgag aagtatggat ttaacatcgg
      601 tacacttgcc aatacggtgt gggtgatcgc gtcgcgggtg gaagataatg tgtcgctgct
      661 tgatatgatc agtaatgccc tggatcagac gttgcagaat acgggggact tgtacatcct
      721 gcatgacgat tttggaaagc tgaatttgtc tttcctcggt gatatgtatg tgccgatcat
      781 gatcgatgcg gaaactggcc agaattatga ctatgagtct tccatcgatg aaaataccta
      841 taaccggatc aaactggttt atgacaatga ggatgccggg aagcgggagg tttatatcgc
      901 acaggattca tccaatatca acaggtgggg gattttacag tattttgatg cgctacaaaa
      961 gggagaaaac gggcaggcaa aggcggacgc gttattacag ctttacaata aggagacgcg
     1021 gacgctgaca gtcaaggatg ccgccggaga ctcacgggtg cgcggtggat cgctcgtcgt
     1081 ggtgcagctt gatctcggtg acgtgaagct tcagaatctc atgttggtgg aaaaatgcgt
     1141 tcacaagtac ggcgagagca aacacacaat ggatttgaca gtatcgggag gtgattttag
     1201 tgcatgacgc aaatgatttt gtccgggcgg tgcagcaggt atcgacgaat gcgaatgatg
     1261 ccgggtatcc ggctacggtg atgtccggga cggtaacatc tgccagccct ttaaaaatca
     1321 aaatcgaaca gagatttgag atcagcggaa gcatgctgat tctgccggaa catttgaaag
     1381 agcgtgaaat caaggtgaca gtaaagccga cgcataccga ggacggcggt acgccggagc
     1441 ataaccatga atatggcggc gaattaacgg tgacggtaca tagcggtctg agtattggtg
     1501 acagcgtgca ggtggtccgg cagcagggcg ggcagaaata tcttgtaatc gggaaggtgg
     1561 tgtaagcatg ataccggtat caaaccagtt gaaaaacgtc gaagtggtag aacagccgtc
     1621 cctctgtccg agaatgatcg tggaaagtga acggatcata gggcagtgcg atgatgtcga
     1681 agctattaag caggcgatct ataacattct gaataccgag cggtatcagt atattatttt
     1741 ttcgtgggac tatggtgtgg aacttaagga tctgttcgga aaaccaatcg attatgttat
     1801 gccggaggta gaacggcgca tcacggaggc tctggtgcag gatgacagga ttgattcctg
     1861 cgacagtttt gagtttgaga aaaagggaag aaaattgctg gttacgttcg ttgcacatac
     1921 gaaatttgga agcgttccgg cacagaagga ggtggatgta taagtgtacg aggagcagac
     1981 gtttgatgca attatgcaga ggatgcttga gcgcattccg gatacgctgg ataagagaga
     2041 aagcagtcct gtatatatgg cgcttgcacc ggcggcggtc gaattggcat cattgtatgt
     2101 tggatttgat tgcatgctgg cggagacatt cggcgataca gcatcgcggg agtacctgat
     2161 ccggttatgt gcggataggg gtatcacacc caagacagct acgtatgcgg tactggaatt
     2221 agagacggat gtggaggtac cggtcggaac acggtttacc ggcggggatc acttttataa
     2281 ggtaactgca agcggacagg taacctgtga gcagccgggg gcagccggga acgaatacct
     2341 gggggatgtt atcccggtgg agtatgtgat gggactcaca actgcaaaac ttacgcgtgt
     2401 gttgatctat ggagaggacg acgaggatac cgaagttctt agattgcggt atcaggaatc
     2461 tttcaatgaa cgggcttttg ccgggaatgc aaaggactat catgacaaaa cactgggaat
     2521 agcaggagtt ggcgctgtta aagtgatccg ggcatggaat ggtccgggaa cagtcaagct
     2581 tgtgatatta gacagtgttt tcggaaaggc aacggatgta ttgattcaga cggtgcagaa
     2641 agaatttgac ccgaataagg acgggcatgg cgatggtctt gcaccgatcg ggcacgcggt
     2701 gacagttgac accgcatccg aagtcactgt taatattgcg gcgacgataa cctatgataa
     2761 cggatatgac cttaatacct gcaagaccca gatcgagaca gccatagagg agtattttgc
     2821 cggacttaga aagaactggg aaaatcagtc gaaactggtt gtgcggattg cgtctattga
     2881 tgcagcgatc atgggagtga aaggcgtggt agatgtgacc ggtacaacgc ttaacggcgg
     2941 gggaaacgtc gaattaacag aatatgagat cccggtactg ggggtggtta cttatggctg
     3001 atcgttatat caatcttaag gaactgctcc ctttgtattt gcaggcgtat aaggaacttg
     3061 ccgcacctat ggatgcggaa acaccggagt tccagataat tgaggcggaa cataacagga
     3121 tcattgccaa ccgctatatc gtgacctgcg atgaggaggg cattgttcgg tacgagcaac
     3181 tcatgggaat ccagcccaag gcagatgata cgttggaaga ccggattttc agatgcataa
     3241 caaagtggaa tgtgtgtctg ccgtacaatt atgctttcct cgatcaaaag ttaagggaac
     3301 tatgtggagc tgaatatacg ttggatctgg acattgctgg ccagaccgta acagttaaag
     3361 tgggacttgc gcagaagaat cagtatgatg tggtcgcgga aatgctggaa gagatagttc
     3421 catgcaactt acagttgaat ctttctttac tgtacaatca gtaccaggcg cttaaaccat
     3481 atccacatat tattctggcg cagtttacac attgggaatt gcggaatttg agcatccccc
     3541 ggaatttaag tgctgcggta gagaatatag cggcgtatgc agtggatgat ctggcacgct
     3601 tcacagtgga agaggttgcg gatataggaa tcagaaagaa ggtatgagat gaaacttacg
     3661 gatttattca aattcaagct gtttgagaga aaagacgttg cagatctggc ggtggtcaat
     3721 gagaacttcc aggcggcgga gagcgagatt gacaagcggc tgctcaaaac tgcggtgcag
     3781 aacacgaaca cagtcacaga agcgggatat gcactggatg ccagacaggc gaatccgaat
     3841 gttaagggat cgctggctga actgattgcg gtgttgagcg aattgttgac aagtcataaa
     3901 agctctggcg atcatgatag cagatattat acaaaaacag atatgaatac gatgcttgcc
     3961 agaaaagctg atagcgagca caaccatgac aaggcgtatt atacaaaagc atttgttgat
     4021 ggtctgcttg cgttgaaagc agaccttaca cattatcacg ataacaggta ctatactgaa
     4081 gcggagacgg atgcaagaat ggcaaaggcg gctcgatatg tgggcttata tgaacaggag
     4141 attacgttgg cagcgggcgg ggaattttac caggcaattc caagtacgta tcagaatggg
     4201 ggatatattt attttataaa ctgctcgggt aactcgttga atttcacggc caacatggaa
     4261 gctctgctgg atggtgtgga agaaggcact gtgaagtgga agaccattgt ggagaacttc
     4321 tatccggacc tggatgcagc agtgaaaaaa gctgagaaag atctggcaga ggtgacgatc
     4381 gcggatgaag aatccgacga agtctgtgaa ctgtgcggca gacgcatggt gatcaaatac
     4441 ggacctcacg gcagatttct ggcatgcccc ggattccctg agtgccgcaa
//
LOCUS       DBMDQJ010000111         4457 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7405_length_4457_cov_1.544766, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000111 DBMDQJ010000000
VERSION     DBMDQJ010000111.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7405_length_4457_cov_1.544766"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            210..911
                     /locus_tag="ACZO9R_03335"
     CDS             210..911
                     /locus_tag="ACZO9R_03335"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912138.1"
                     /translation="MAYIEFNNVTKEYKTGETSIKALDGASFSVEKGELAVILGSSGA
                     GKTTALNILGGMDVPTAGEIKVDGRDIAKYNKKQLVGYRRTDIGFVFQFYNLVPNLTA
                     IENVELAAQVCRNSLDAGEMLTKVGLADRKNNFPAQLSGGEQQRVSIARAIAKNPKLL
                     LCDEPTGALDYNTGKQILQLLQDTCRNDGITVLLITHNSALAPMADRLIRFKSGKVTE
                     ITCNAHPTPIAEIEW"
     gene            941..4402
                     /locus_tag="ACZO9R_03340"
     CDS             941..4402
                     /locus_tag="ACZO9R_03340"
                     /inference="COORDINATES: protein motif:HMM:NF024116.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR0912139.1"
                     /translation="MKSAYNKEIWRAIRKGKKRFFSIMLITILGVGMLTGLRAACMDL
                     RYSADQFYDRQGLYDISVVSTLGLTEKDVDALKALDGVADAVGVYSETVHAKVNDHEV
                     SIAVCTLNDRGINQPYVLSGRLPESADETAVTTAFADETGCGIGDVIAVDTTDDEESS
                     LLCSRFTVTGIVVDVQDVNNAKGATAFRSGQSEDDTVFILPEAADSDIYTQVCLTVDG
                     AAELFCYSDAYEEKVAQVTDAIETQIKENREQARYVEVTDEAKEKLADAKAEAEESFA
                     EAEQQLSDARAELDKGWQELSDGQEEFDAEKQQAEDGFAEARDEIAAGKQQIADGSAQ
                     LDAAEQQLSEGEAQVASGKQTLSAKEEETYAQLDAARAQLTESSKQAAETEAALEAQA
                     QEVIQAYGEAWPAEAWDAYVQAAVEAYLPVARAVVAEQDASQAQAAVPGLVADSQSAF
                     LEALQQPTADQLVQLAMGLGNVRATIQALGEAQAQLDTAEQTARQQFDAAWNELNEGE
                     AQIIFGKSQIADKRAELENGSMALSEGEAQLAQKEQETAAQIKDGEENLAEGREKLLE
                     GETEYADGKAEYEENLADFNETIAEEEQKIADIDMTQWYVQDRTSLSGYANVKSDAAS
                     IEAIGTVFPMVFFVVAILISLTTITRMVEEDRGLIGTYKALGFTDREIRRKYLVYACA
                     ASVSGGILGNVCGFVLLPEIIFRIFDTMYLFPEYLLRFMPLLGCGGAALFAAGIVIAT
                     ACACKAELVHMPAVLMRPKAPRAGSRVLLERITPVWKRLSFLNKVTARNLFRYKKRLF
                     MTVAGIMGCMALLLFGFAVRDSVTDLLPRQYENVYRYDVMAAGAASDNDKILSYVNGD
                     PNVQSALNVQISTVKLKNAAGKEEKAQMIVVPGDEPLAEYIGLYNLKDEEIGLLDGEI
                     LVTQNAANVLGFAAGDQVEIQDQDLNQREVTVSGLVRNYLGNNVYMTEKTYESLFGDY
                     EPNGVFLKLSEECSDEIAYADDLGGKEGIVSYTSKQELSDEFSDAFTLINMVVYIVII
                     MAAALAFVVLFTLSTTNISERSRELATIKVLGFFDREVHLYVNKETMILTAIGIVCGI
                     PLGYAFAQTLTYVLNMPSIYLAVSLHGKSYAMAAGLSFLFAILVDLLTNRSLDEIDPV
                     EALKSVE"
BASE COUNT         1104 a          956 c         1428 g          969 t
ORIGIN      
        1 ctgctttttc atgaaaacct cccggtataa gatttgtgtt cattttgtct tcacgtaaat
       61 tatagcacga caacgtgttg tcttcaattt tttaacacgt gcggagaatc tgttgattga
      121 tgaaaggcag cgcggggagt acattaggca tagaaaatgg aatctgcata gagataaaaa
      181 acagagtctg ggaagaaagg cggcctttta tggcatatat cgaatttaat aatgtaacga
      241 aggaatataa gacaggtgag acttcgatca aagcgctgga tggagcctca ttttctgtgg
      301 agaagggcga actggcagtg attctcggtt cctccggtgc agggaagacg acggcgctca
      361 atattttagg cggaatggat gtgccgactg cgggcgagat caaggtggat ggcagagata
      421 ttgcaaagta taacaaaaag cagctcgtcg gataccggcg taccgatatc gggtttgtat
      481 tccagttcta caatctggtg ccgaatctca cagcgataga gaatgtggaa cttgcggcgc
      541 aggtgtgcag aaattcgctc gatgccgggg agatgctcac aaaagtggga ctggcggacc
      601 gcaaaaataa ctttccggcg cagctttccg gaggagaaca gcagcgggtg tctatcgcgc
      661 gtgcgatcgc caagaatccg aaactcctgc tctgcgatga gccgacggga gcgcttgact
      721 ataacaccgg aaaacagatt ctgcagctgc tgcaggatac ctgccggaac gatgggatta
      781 cggtacttct gattacgcac aactctgcgc tggcaccgat ggctgaccgc ctgatccgct
      841 ttaagagcgg aaaggtgaca gagattacct gcaatgcgca ccctacgccg atcgcggaga
      901 tcgagtggta gcggggcaga agagaaaggc agggatactg atgaagagtg catataataa
      961 ggagatatgg cgggcgatcc gcaaggggaa aaaacggttt ttctccatca tgctgatcac
     1021 gattctgggt gtcggaatgc tcaccggatt gcgggctgcc tgcatggatc tgcgctattc
     1081 ggcagatcag ttttatgaca gacagggact gtacgatatc agtgtcgtct cgacgctcgg
     1141 acttacggag aaggatgtgg atgcgttaaa ggcactggac ggtgtcgcgg atgcggtcgg
     1201 cgtctacagt gagacggtac atgcgaaagt aaacgatcat gaggtcagca ttgcagtctg
     1261 cacgttgaat gacaggggaa tcaatcagcc gtatgtgttg tccggcaggc tgccggagag
     1321 tgcggatgag accgccgtta caaccgcgtt tgccgatgag acgggatgcg gcatcgggga
     1381 tgtcatcgcg gtggatacaa cagatgatga ggagtcttcg ctgctctgca gccgctttac
     1441 cgtaacggga attgtggtgg atgtgcagga tgtcaacaat gcaaagggag cgacggcgtt
     1501 ccgctccggg cagtcggagg atgacactgt gttcatcttg ccggaggcgg cagacagcga
     1561 tatttacaca caagtctgcc ttaccgtcga tggtgctgca gaattgttct gctattccga
     1621 tgcctatgag gaaaaggtcg cacaggtgac ggatgcgatt gaaacgcaga ttaaggaaaa
     1681 tcgggagcag gcacggtacg tggaagtcac ggatgaggcg aaagagaagc ttgcggatgc
     1741 gaaagcggag gcggaagagt cctttgctga ggcagagcag cagctttccg atgcgagagc
     1801 agagctggat aagggctggc aggaactttc cgacggccag gaggaatttg atgcggaaaa
     1861 acagcaggcg gaggatggtt ttgcggaagc gagagatgaa attgccgcgg gaaagcagca
     1921 gattgccgat ggcagcgcgc agctggatgc agcggagcag cagctttcgg aaggcgaggc
     1981 gcaggtggca tccggtaagc agacgctttc ggcaaaggaa gaggaaacct atgcacagtt
     2041 agacgcggca cgcgcacagc ttacagagag cagtaaacag gcagcggaga cggaggcggc
     2101 attggaagcg caggcgcagg aggtcatcca ggcgtacggg gaagcatggc ccgctgaggc
     2161 ctgggacgcc tatgtgcagg cggcagtgga ggcgtattta cccgtagcaa gagcggtggt
     2221 cgcagagcag gatgcctcgc aggcacaggc ggcggtgccg ggactggtgg cggacagcca
     2281 gagtgcgttt ttggaggcgc tgcagcaacc cacagccgat cagctggtac agcttgcaat
     2341 gggactggga aatgtgcggg cgacaatcca ggcgctcggc gaggcacagg cgcagctgga
     2401 tacggcagaa cagacggcac ggcagcagtt tgatgcggca tggaacgaac tgaacgaagg
     2461 ggaagcacag atcatcttcg gaaaatcgca gattgccgat aagagagcag agctggagaa
     2521 cggcagtatg gcgctttccg aaggcgaggc gcagctggcg caaaaggaac aggagactgc
     2581 ggcgcagata aaggacggtg aggaaaatct cgccgaagga cgggaaaagc tgcttgaagg
     2641 tgagacggaa tacgcggacg gcaaggcaga atatgaagaa aatcttgcgg acttcaatga
     2701 gacgattgcg gaggaagagc aaaagattgc ggacattgac atgacacagt ggtatgtaca
     2761 ggatcgcacg tctctgagcg gttatgccaa tgtgaagagc gatgcggcgt cgattgaggc
     2821 gatcggtacg gtgttcccga tggtattttt cgttgttgcc attctcatca gcctgacgac
     2881 gataacccgt atggtggagg aggaccgcgg tctgattgga acctacaaag cgctcggctt
     2941 tacggatcgt gagatccgca gaaaatatct ggtgtatgca tgtgcggcga gtgtgtccgg
     3001 cggaattctg ggaaatgtgt gcggatttgt tcttctgccg gagattattt tccggatttt
     3061 tgatacgatg tatctgttcc cggaatatct gctgcgcttt atgccgctgc tcggatgtgg
     3121 cggagctgca ctttttgcgg ccggaattgt catcgctacc gcctgtgcat gtaaagcgga
     3181 acttgtacac atgccggcag ttctgatgcg gccgaaagcg ccgcgcgcag gctcccgcgt
     3241 actgctggag cgcatcacgc cggtatggaa gcgcctgtca tttctcaata aagtgaccgc
     3301 ccgcaatctg ttccgctata aaaagcgtct tttcatgacc gtagcgggaa ttatgggatg
     3361 catggcgctc ttacttttcg gattcgcggt gagggattcg gtgacagacc tgctgccgcg
     3421 ccagtatgag aatgtgtacc ggtatgatgt catggcggcg ggggcagcat ccgacaatga
     3481 caagattctt tcctatgtaa atggggatcc gaatgtgcaa agtgcgctga acgtgcagat
     3541 ttccacggtg aagctgaaga atgctgccgg gaaggaagaa aaggcacaga tgattgtcgt
     3601 gccgggggat gagcctcttg cagagtacat tggtctgtac aatcttaagg atgaggagat
     3661 cggccttttg gacggcgaga ttctggtgac gcagaatgcg gcaaatgtgc ttgggtttgc
     3721 ggccggcgat caggtggaga ttcaggatca ggatctgaat cagcgggaag tgacggtaag
     3781 cggtctcgtc cgcaattacc tcggcaataa cgtctatatg acagagaaaa cctatgaatc
     3841 gctgtttggc gattatgaac cgaacggagt atttttaaag ttgtcggaag aatgttcgga
     3901 tgaaattgcc tatgcagatg atctcggcgg aaaagagggt atcgtgtcct acaccagcaa
     3961 gcaggagctt tcggatgagt tctcggatgc gttcaccctg atcaatatgg tggtctacat
     4021 tgtgattatc atggcggcgg cgctcgcgtt cgtggtttta tttacgctgt ctacgacgaa
     4081 tatttccgag cggagcagag agctggcgac gattaaggtg ctgggatttt ttgaccgtga
     4141 agtgcatctg tatgtcaaca aagagaccat gatactcacg gcgatcggca ttgtctgcgg
     4201 aattccgctg ggatatgcgt ttgcgcagac cttaacgtat gttttgaaca tgccgtcgat
     4261 ttatctggcg gtgtcccttc acgggaaaag ctatgccatg gcggcaggcc tctcattttt
     4321 gtttgcgatc ctggtggatc tgctgacgaa ccgctccctg gatgaaattg atccggtgga
     4381 agcgttaaag agtgtagaat agaaaaagcg ccggcaccga tgtgtgaagt tcatcggtgc
     4441 cggcgctttc tttttgt
//
LOCUS       DBMDQJ010000112         4440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7438_length_4440_cov_1.640470, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000112 DBMDQJ010000000
VERSION     DBMDQJ010000112.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7438_length_4440_cov_1.640470"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="ACZO9R_03345"
     CDS             complement(<1..451)
                     /locus_tag="ACZO9R_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR0912140.1"
                     /translation="MNVILAKSAGFCFGVKRAVDMVYERILEKEPLYTFGPIIHNEEV
                     VRDLEEKGVVVVNDVDELAGKPQGTVIIRAHGVERGVCEKIQTLGFSIVDATCPFVLK
                     IHRLVERYSGEDCHIVIVGNASHPEVKGIKSWSNAEDTRVIGTREEAE"
     gene            complement(457..1119)
                     /gene="cmk"
                     /locus_tag="ACZO9R_03350"
     CDS             complement(457..1119)
                     /gene="cmk"
                     /locus_tag="ACZO9R_03350"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR0912141.1"
                     /translation="MSMNIAIDGPAGAGKSTIAKKLAKKLTFVYVDTGAMYRAMAYYF
                     LQNGIAPDDEQAIAAACPKVDVTIVYENGEQQVLLNGENVNGVIRTEEVGNMASATSV
                     YPVVRTKLVELQRQLATKTDVIMDGRDIGTCVLPDAQVKIYLTASVATRAKRRYDELT
                     AKGTQCNLEEIEKDIEDRDYRDMHRETSPLKQAKDAVLVDSSEMNIDEVVDAIYQIYA
                     RV"
     gene            complement(1138..2367)
                     /locus_tag="ACZO9R_03355"
     CDS             complement(1138..2367)
                     /locus_tag="ACZO9R_03355"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR0912142.1"
                     /translation="MSKVIVVGGGPAGMFAAIAAAERGHEVVLLEKNEKLGKKLYITG
                     KGRCNITNAGDMDTLFANVMTNPKFLYSAFYDYDNQRVIDFFEENGLATKVERGNRVF
                     PVSDHSSDVIATLQRVLKRTGVMVQLHAEVRQILTGEGAVRGVKLADGSVLEAERVIL
                     ATGGFSYQTTGSTGDGYRFARELGHTVTQITPSLVPFYVKETCAARMQGLALKNVQVR
                     IRDGKKLLYDEFGEMLFTHFGVSGPLILSASAAIKPGRVEKELAMEIDLKPALTEEQL
                     DARLLREFEEAKNKQFKNAVVHLFPAKMIPVMLEAGAIDPEKKVNEITREERMGFVHL
                     IKAFPLTLCGVRDFNEAIITRGGVSVKEVNPSTMESKLVPGLYFCGELLDLDAMTGGF
                     NLQIAWSTGHLAGESIE"
     gene            complement(2360..3292)
                     /locus_tag="ACZO9R_03360"
     CDS             complement(2360..3292)
                     /locus_tag="ACZO9R_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HIR0912143.1"
                     /translation="MSNTNDLSNRINDAYSKLSKGQKRLATYITDNYDKAVFLTAAKL
                     GEVVGVSESTVVRFATHLGYKGYPEFQSALEELVRNKLNSIQRMEVTYGRISQSKILE
                     TVLKSDADKIHSTLEKIDQNAFELAVDTILHAKHIYIIGIRSCAPLASFMAFYFTLMF
                     PNVHLIQTSSSSEIFEQMVRIGKEDVIIGISFPRYSMRTLKAMEFANNRSAKVITLTD
                     SVHSPMNLYSSCNLIADSDMASIVDSLVAPLSVINALIVALCMKKQNEVAKTLEMLED
                     IWDEYQVYENDEINYIDDSIKMRYGKVGDAELYE"
     gene            3454..3936
                     /locus_tag="ACZO9R_03365"
     CDS             3454..3936
                     /locus_tag="ACZO9R_03365"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HIR0912144.1"
                     /translation="MRDITPATNFIHTLMQSPPDASAWIAGGNNFPSISGLARFYATE
                     YEGTLVEVEVFGLPNVAAPGSSNFYGMHIHENGVCVSPFTSAGAHYSRTPAEHPNHSG
                     DMPPLLGNQGYAWMAFYDKRFTIPEIIGKSLIIHRMPDNFTTQPAGDSGERIACGVIK
                     "
     gene            complement(4037..>4440)
                     /locus_tag="ACZO9R_03370"
     CDS             complement(4037..>4440)
                     /locus_tag="ACZO9R_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HIR0912145.1"
                     /translation="VAKFKKRMRELTCRSWGVSNSYKVGKLNQLIRGWINYFKIGSMK
                     TLCRELDGNIRYRLRMCIWKHWKTPQNKEKNLVKLGVPRWAAHKVANTGNRYAHMCHN
                     GWIQKAISIKRLTSFGLVSMLDYYTERCTTC"
BASE COUNT          982 a         1334 c          927 g         1197 t
ORIGIN      
        1 tttcggcctc ttctcgggtt ccgatcacgc gcgtatcctc tgcattcgac cagcttttga
       61 tgcctttcac ctccgggtgg ctggcattcc cgacgatcac aatgtgacag tcctccccac
      121 tgtagcgctc caccagccgg tgaatcttta acacaaacgg gcaggtcgcg tccacgatgg
      181 aaaagccgag cgtctgtatt ttctcgcaga cacctctctc cacgccgtgt gcccggatga
      241 ttaccgtccc ctgcggcttc cccgcaagtt cgtccacatc gtttaccacg acgacgccct
      301 tttcctccag atcacgcacc acttcttcat tgtggatgat cggtccgaac gtgtagagcg
      361 gttctttttc caaaatccgt tcatatacca tatccacggc acgttttacc ccgaagcaga
      421 agcccgcgct ctttgccaag attacattca tattctctat accctcgcat aaatctgata
      481 gattgcgtcc accacttcgt cgatgttcat ctcggagctg tccactaaca ccgcatcctt
      541 ggcctgcttt aacggcgacg tctcccggtg catatcgcgg taatcgcggt cttcaatatc
      601 tttctcgatc tcttccagat tgcactgggt tcccttggcg gtcagttcat catatctgcg
      661 ctttgcgcgc gttgcaacgc ttgccgtgag atatattttt acctgcgcat ccggaagcac
      721 acaggtgccg atatcccttc cgtccatgat gacgtccgtc tttgtcgcaa gctgccgctg
      781 cagttccaca agctttgtgc gcacaaccgg atagacactg gtcgctgatg ccatattgcc
      841 gacttcctcc gtccgaatga cgccgtttac attctctccg tttaagagca cctgctgttc
      901 gccgttctca tacacaatcg tcacgtcaac cttcgggcag gctgccgcaa tcgcctgctc
      961 atcatctggt gcaatgccgt tctgtaaaaa atagtatgcc atcgcacggt acattgctcc
     1021 cgtatccaca tagacaaacg tcagcttttt cgccagtttt ttcgcaattg tgctcttccc
     1081 ggcgcctgcc ggtccgtcaa tcgcaatatt catactcatc tcttcttctc tcctcttcta
     1141 ttcgatactc tcacccgcca gatgcccggt cgaccaggca atctgcagat taaatccgcc
     1201 ggtcatcgca tccagatcca atagttcccc gcagaaatac aatcccggca caagctttga
     1261 ttccatcgtg gaagggttca cttcctttac gctgacaccc ccgcgtgtga tgatcgcttc
     1321 attgaaatct ctgacaccgc agagcgtcag cggaaacgcc ttgatcagat gcacaaatcc
     1381 catccgctcc tctctcgtga tctcgttgac cttcttctcc ggatcaatcg ctccggcttc
     1441 taacatcacc ggaatcatct tcgccgggaa cagatgcacg acggcgtttt taaactgctt
     1501 attctttgcc tcctcgaatt cacgcagaag tctcgcatcc agctgttcct ccgtgagtgc
     1561 cggctttaag tcgatctcca tcgccagttc cttctccacg cggcccggct tgattgccgc
     1621 actggcgctt aaaatcagcg gtccgcttac cccgaaatgg gtaaagagca tctcgccgaa
     1681 ttcgtcatac aacagctttt ttccgtcccg gatgcgcacc tgcacatttt tcagcgccag
     1741 cccctgcatc ctcgccgcgc aggtctcctt cacgtagaac ggaaccagtg acggcgtaat
     1801 ctgtgtcacc gtatgtccga gttccctcgc aaaacggtag ccgtctccgg tcgaaccggt
     1861 cgtctgataa gaaaatcctc cggttgcaag aatcacgcgc tctgcttcaa gaacgcttcc
     1921 gtcggcaagc ttcacgccgc gcaccgcgcc ttccccggtg aggatctgcc gcacttccgc
     1981 atgaagctgc accataacac ccgtgcgctt tagtacccgc tgtagcgtgg cgatcacatc
     2041 cgaagaatga tccgataccg ggaatacacg gttgccgcgc tccaccttcg tcgccagacc
     2101 attctcctca aaaaaatcaa tcactctctg attgtcataa tcgtaaaaag cgctgtacaa
     2161 aaacttcggg ttcgtcatca cattggcaaa cagggtgtcc atatcccccg cattcgtgat
     2221 gttgcaccgc cctttgccgg tgatgtagag ctttttccca agcttttcat ttttctcgag
     2281 caaaacgacc tcatgtcccc gctccgccgc cgcaatggca gcaaacattc ccgccggccc
     2341 gccgccgact acgatcacct tactcataca gttccgcatc tcctactttt ccataccgca
     2401 tcttgatgga atcatcgata taattgatct catcgttctc ataaacctgg tattcgtccc
     2461 agatatcctc taacatctca agcgttttcg ccacctcgtt ctgctttttc atgcagagcg
     2521 ccacaatcag cgcattgatc accgacagcg gggcaaccag ggaatccaca atggaagcca
     2581 tatcgctgtc cgcaatcaga ttgcaggaag aatagaggtt catcggggaa tgcacgctgt
     2641 cggtgagtgt gattaccttc gcactccggt tattcgcaaa ttccatcgcc tttaaagtac
     2701 gcatggaata acgcggaaaa ctgattccga tgatcacatc ctccttgccg atgcgcacca
     2761 tctgttcaaa aatctcgctc gaactgcttg tctgaatcag atgaacgttc ggaaacatca
     2821 gggtaaaata aaaagccatg aagctggcaa gcggcgcaca gctccggatt ccgatgatat
     2881 agatatgttt ggcgtgcagg atcgtgtcaa ccgcgagttc aaatgcgttc tgatcaatct
     2941 tttccagtgt ggaatgaatc ttgtccgcat cggatttcag caccgtctcc agaatcttcg
     3001 actggctgat cctgccgtaa gtaacctcca tgcgctgaat ggagttcagc ttattgcgca
     3061 ccagttcctc cagcgcactc tggaattccg gatacccctt atatcccaga tgcgttgcaa
     3121 agcgcaccac cgtggactcg ctcacaccta caacctcgcc cagctttgca gcggtcagaa
     3181 ataccgcctt gtcataattg tccgtaatgt aggttgccag ccgtttctgt cccttgctca
     3241 gcttactgta agcatcattg atgcggttac tcaggtcatt tgtattgctc atgcttctcc
     3301 tcacttctgc ggaattgtga tccgctcaat gtttttatta tatcaaatac atggtgtttt
     3361 gcaagaaatt atgcatggaa ctttccttac atgcagcgca tttttctgcc gctgcatata
     3421 ataaaccaac gacactcttc cattgaggca gctatgcgcg atatcacacc agcaaccaat
     3481 tttattcata cactgatgca gtctccgccg gatgcatccg cctggattgc cggcggcaac
     3541 aattttcctt ccatctccgg gcttgcccgg ttctatgcca cagaatacga aggaactctc
     3601 gtggaggttg aagtcttcgg tcttccgaac gtcgcggctc ccggctccag taatttttac
     3661 ggcatgcaca tccacgaaaa cggcgtgtgc gtctccccgt ttaccagtgc cggtgcccat
     3721 tacagccgta cgccggccga acacccgaat cacagcggcg acatgcctcc gcttctgggc
     3781 aaccagggtt atgcctggat ggcattttat gacaagcgtt ttacgattcc ggaaattatc
     3841 ggaaaatccc tcatcattca ccggatgccg gataacttta caacgcagcc cgcgggcgac
     3901 tccggcgaac ggattgcctg cggtgtgata aaatagcaaa aagggcagat cgagtaggag
     3961 gaatttctct taaataagaa attccgacct ctcacaccac cgtgcgtacc gttcggtaca
     4021 cggcggttca atcaacttaa caagttgtac acctttcggt gtagtaatct aacattgaga
     4081 ctaatccgaa tgaggttagt ctctttatgc ttatagcctt ttgtatccat ccattatgac
     4141 acatgtgtgc ataacgattt cctgtatttg ccactttatg tgccgcccat cttggaacac
     4201 cgagcttaac taaattcttc tctttgtttt gtggtgtttt ccaatgtttc caaatacaca
     4261 tgcgtagtct atatctaatg tttccatcaa gttctctaca gagagttttc atactaccta
     4321 tcttaaagta gtttatccat cctctgataa gctgattaag tttccccact ttatagctgt
     4381 tgctaacgcc ccagctacgg caagtgagtt ccctcattct cttcttgaac ttggctactg
//
LOCUS       DBMDQJ010000113         4440 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7439_length_4440_cov_1.498732, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000113 DBMDQJ010000000
VERSION     DBMDQJ010000113.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7439_length_4440_cov_1.498732"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1050
                     /locus_tag="ACZO9R_03375"
     CDS             <1..1050
                     /locus_tag="ACZO9R_03375"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0912146.1"
                     /translation="KFIHIADVHLGVKPDQGKPWSELRARQIWDSFADVIGVAKREKP
                     EFLFVTGDLFHAQPLKKEVREVSRLFGEIPDTQVLLIAGNHDYLREKSYYLADLWPDN
                     VTVFKKEEAEAVDFPEYNTTVYGLSYWHREIRDRLYDDLHPVNHSRCNILLAHGGDER
                     HIPFSAERILQNGFDYIAAGHIHRSGWLMPHKAVMAGSLEPTDCNDTGPHGYWMGTFG
                     AAGADVYFYPLRHCEYCHEMYQADRNTTDRMLWDWAKELLEQRSEYQYFRLYIKGKKD
                     PEYSFDVQEIGKLDRIVDITDQTVPDYDYEKLLEEHADSILGEYIRSMRKKEPGVCTA
                     KALEYGVNALLGYDC"
     gene            1094..2737
                     /locus_tag="ACZO9R_03380"
     CDS             1094..2737
                     /locus_tag="ACZO9R_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0912147.1"
                     /translation="MQIDRAAIFRFGKLADRTVDFAPGINIVYGKNEAGKTTLHAFLT
                     AMLFGLEKGRGRAKGTEGYLRYEPWHAPSYYSGALQFSVGGRPFYLERNFYHKEPRAR
                     LCNLADGEELSVAYGDLGMLLGGVTREAYENTCDIPQCRAVTGAELTGLLAEYLSDMS
                     DGGNAGIRVTRAVEKLEQKKRSLQSQIKSEQEKREQQLQQLTLERRMLEEDCGRLREQ
                     IEKAAADMRAYARMHPQVRDGQRPEDARTAENAGTASDRERRADGNGFPVRSLFLGLA
                     AAVGLAGNAWWYHRAGYAPELFAAAEGVLAILLGIGVAGILRHRSAGQARAGENGKET
                     TEAPESAAAEDAVRIRLAESEKQSRRLLAGLEETLAEKETRRCNLAEQLEVCSGAGAR
                     ERELQLELDAVEMAKNEIIRLSREYGDERRDEINSVVSRYVSAITEGKYDLAEVDETG
                     KLRVQTEGREVLPEALSRGTLEQFYFAFRMAVGSIVTQEEPLPLLLDETFAMYDDDRL
                     RQTLRLLAANGSQTILFTCQRREQKLLEELGIAYHMIEL"
     gene            2813..>4440
                     /locus_tag="ACZO9R_03385"
     CDS             2813..>4440
                     /locus_tag="ACZO9R_03385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR0912148.1"
                     /translation="MAAKKKKKKYRGFWIFVKVQFVLSLLVLGGLGYYVLGGYASEVS
                     SIHKEADSLVRNSTEETFRRYRTSVVYAADGSVISKLNDQGASYYLKRDEIPQSVIDA
                     VVCMEDQRFYQHRGVDFRALVRAAKSLIENRKITQGGSTITMQLARNVFLTQEKTWQR
                     KVEEIFIAQNLEKKYTKDQILEFYLNNIYYGNGYYGIGAAGRGYFDSGVDELDLSQIA
                     FLCAIPNNPTVYDPVTHMDHAIERRDRILGKMRDDGKITQMAYAAAVVENITLKRPEA
                     MEKNNYVETYAYYCATRALMEQAGFVFQYQFATEDEREAYERAYSESYSDCQKRLYTE
                     GYQIYTSFDLQLQEELQVAVDDTLSGFTEKNDEGVYDLQGAAVCIDNDNGYVRAMVGG
                     REQDFDGYTLNRAYQSYRQPGSAIKPLTVYTPSFERNYTPESIVVDEPVEDGPKNANG
                     TYLGNVTVRTAVEKSINTIAWKLYEELTPQVGLSYLENMHFSRLDAADYVPATALGGF
                     TNGVSPLEMAAGYAALANDGMYREPTCIMRITDDT"
BASE COUNT         1140 a          953 c         1461 g          886 t
ORIGIN      
        1 aaatttatac atatcgcaga tgtacatctc ggggtaaagc cggatcaggg aaagccgtgg
       61 agtgagctgc gggcgcggca gatctgggat tcttttgcag atgtgatcgg ggtggcaaaa
      121 agagaaaaac cggagttttt gttcgtgacg ggggatcttt tccacgcaca gccgctcaaa
      181 aaggaggtgc gggaggtcag ccgccttttc ggagagattc cggacacaca ggttcttttg
      241 attgccggca atcatgatta tctcagggaa aagtcgtatt acctcgcgga tttgtggccg
      301 gacaacgtga cggtatttaa gaaagaggaa gcggaagcag tcgattttcc ggaatataac
      361 acgaccgtat acgggctaag ctactggcac agggagatcc gcgacagatt gtatgatgac
      421 ctgcaccctg taaaccacag caggtgcaac atcctgctgg cgcatggcgg cgatgagcgc
      481 catattccgt tttcggcgga gcggatatta cagaacggat ttgattatat tgcggcggga
      541 cacattcaca ggagcggctg gctcatgccg cacaaggcgg taatggcggg aagcctggag
      601 ccgacagact gcaacgacac cgggccgcac ggatactgga tgggaacgtt cggtgcagcg
      661 ggggcagatg tgtattttta tccgctgcgc cactgcgaat attgtcatga gatgtatcag
      721 gcagaccgga acacgacaga ccgtatgctg tgggactggg caaaggagct gttggagcag
      781 agatcggagt atcagtattt ccgtctgtat atcaaaggga aaaaagatcc ggagtattcc
      841 tttgatgtgc aggagatcgg gaagctcgac cggattgtgg atattacaga ccagacggtg
      901 ccggattacg attacgagaa gctgctggag gagcacgcgg attcgatact tggggaatac
      961 atacgcagta tgcgaaaaaa agaaccgggc gtctgcaccg caaaggcact ggagtacggt
     1021 gtgaatgcgc tgctcggcta tgactgctga cggaatgcct gaagtatgac aaagagcaaa
     1081 ggatgggaca gacatgcaga ttgacagagc cgcaattttt cgctttggaa agctggcaga
     1141 tagaaccgtg gattttgccc cggggatcaa cattgtatat ggaaaaaacg aagccggtaa
     1201 gacgacgctg cacgcgtttc tgaccgcgat gctgttcggg ctggaaaaag gccggggacg
     1261 ggcaaagggg acggagggct atctgcgcta tgagccgtgg catgcaccgt cctattacag
     1321 cggtgcgctg cagtttagcg tcggaggcag accgttttat ctggaacgca atttttacca
     1381 taaagaaccg agagcacgcc tctgcaacct ggcggacggg gaggaactgt ccgtcgcgta
     1441 cggagatctt ggaatgcttt taggcggggt cacccgggag gcgtatgaga acacctgtga
     1501 cattccgcag tgccgggcgg tgacgggagc agagctgacc gggcttctgg cagagtatct
     1561 ttccgatatg tcggacgggg gcaatgcggg aatccgcgtg acgcgtgcgg tggaaaaact
     1621 ggagcagaaa aagagaagcc tgcagtcgca gatcaaaagt gaacaggaga agcgggaaca
     1681 acagctgcag cagcttacct tggagcggcg catgctcgag gaggactgcg gcaggcttcg
     1741 tgagcagatc gagaaggcgg cagcggatat gcgggcgtat gccaggatgc acccgcaggt
     1801 gagagacgga cagcgaccgg aagatgcccg aactgcggaa aatgcgggga ctgcgtcaga
     1861 cagggaaagg agagctgacg gaaacggttt tcctgtgaga agcctttttc tgggactggc
     1921 ggcagcagtg ggactggcag gaaatgcatg gtggtatcac cgcgcgggtt atgcgccgga
     1981 gctgtttgcg gcggcggaag gcgttcttgc cattctgctc ggcatcggag tggcaggcat
     2041 actgcgccat aggagcgcgg ggcaggcgcg tgcaggagaa aatgggaagg agacgacgga
     2101 ggcgccagag agcgcggcag cggaggacgc ggtgcgcatc cggcttgcgg agtcggaaaa
     2161 gcagagcaga cgcctgcttg cagggttgga ggaaacactc gcggagaagg aaacgagacg
     2221 atgcaatctg gcggaacagc tggaagtatg cagcggagcg ggcgcacggg agagagaact
     2281 ccagctggaa cttgatgccg tcgagatggc aaaaaatgag atcatccgcc tgtcaaggga
     2341 atacggggac gagcggagag atgagatcaa cagtgtggta tccagatatg tctctgcgat
     2401 tacagaggga aaatacgatc tggcggaagt ggacgaaacg ggaaagctcc gcgtgcagac
     2461 ggagggaaga gaggttttgc cggaggcgct cagcagagga acgctggaac agttctattt
     2521 tgcgttccgc atggcagtcg ggagcattgt gacacaggaa gaaccgctgc cgctgcttct
     2581 ggatgaaacc tttgcaatgt atgacgatga ccgtctgcgg cagacgctgc ggcttctggc
     2641 ggcaaacgga tcgcagacga tcctgtttac ctgccagaga cgggaacaga agctgttaga
     2701 ggaactggga atcgcatacc atatgataga gttatagtac cgtttgcgtt atgtgcaaag
     2761 tggggcagga cggcactgcg cgacacgcga gaaaagggca gggaggaata cggtggctgc
     2821 aaagaaaaag aagaaaaaat atcggggatt ctggatattt gtcaaagtac agtttgttct
     2881 ttccctgctg gtgctcggcg ggctgggcta ctatgtcctt ggcggatatg cctcagaggt
     2941 gagcagcatc cacaaggaag cggacagtct ggtacggaat tccacggagg agacgttccg
     3001 gagataccgc acaagcgttg tgtatgcggc ggacggcagt gtgatatcca agctcaatga
     3061 ccagggagcg tcctattatc taaagcgcga cgagataccg cagtccgtga tagatgcggt
     3121 ggtctgtatg gaggatcagc ggttttacca gcatagaggt gtggacttca gagcgcttgt
     3181 gcgcgcggcg aaatcactga ttgaaaaccg gaagatcacg caggggggaa gcacgatcac
     3241 aatgcagctt gccagaaatg tatttctgac gcaggagaag acctggcagc gcaaggtgga
     3301 agagattttt attgcacaga atctggagaa aaaatatacc aaggatcaga ttctggaatt
     3361 ttatctgaat aatatttatt atggcaacgg ctactatgga atcggggcgg caggcagagg
     3421 ctactttgac agcggggttg atgaactgga tctctcgcag atcgcgtttt tatgcgcgat
     3481 tccgaataat ccgaccgtgt atgacccggt tacccatatg gatcatgcga ttgagcggcg
     3541 cgaccggatt ctggggaaaa tgcgggatga cggtaagatt acacagatgg catatgcggc
     3601 ggcggtggtg gaaaatatta ccttaaagcg gccggaggcg atggaaaaaa ataattatgt
     3661 ggaaacatat gcctattatt gtgcgacgcg tgcactgatg gagcaggcag ggtttgtgtt
     3721 tcagtatcag tttgcgacgg aggacgagag ggaggcttac gagcgggctt acagcgaatc
     3781 ctacagcgat tgccagaagc ggctgtacac ggagggctat cagatttata cgtcttttga
     3841 tctgcagctt caggaggaac tgcaggtagc agttgacgat accttatccg gttttacgga
     3901 gaaaaatgac gagggtgttt atgatctgca gggggcggca gtctgcattg acaacgataa
     3961 cggctacgtg cgcgcgatgg tgggcggcag ggagcaggat tttgacggat acacgttaaa
     4021 ccgcgcctac cagagttacc gtcagccggg aagtgcgatt aagccgctca cggtatacac
     4081 gccgtcgttc gagcggaatt atacgccgga gagcattgtt gtggatgaac cggtggaaga
     4141 cggaccgaaa aatgcgaacg gtacctacct tggaaatgtg acggtgcgca ccgcagtcga
     4201 aaaatcaatc aataccattg cgtggaagct ctatgaagaa ctgacgccgc aggtcggtct
     4261 gtcctatctt gagaatatgc atttttccag attggatgcg gcggattatg taccggcgac
     4321 agcgctgggc ggttttacca acggtgtgtc gcctctggag atggcagccg gatatgcggc
     4381 gctcgccaat gacggaatgt accgcgagcc gacctgtatt atgcggatta cggatgacac
//
LOCUS       DBMDQJ010000114         4427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7458_length_4427_cov_1.627832, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000114 DBMDQJ010000000
VERSION     DBMDQJ010000114.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7458_length_4427_cov_1.627832"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..729
                     /locus_tag="ACZO9R_03390"
     CDS             <1..729
                     /locus_tag="ACZO9R_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR0912149.1"
                     /translation="SKTEGGCSGNRKYMIIETALYQDVLEHSSIFRQLPKDKYPNVLR
                     CLNARVVDYPQDASIAALGDSSGCSGILLTGMLEEYLVDENGNQIIINHLRSGDVFGA
                     ESVCAGQVASHVYIHTVEKSKILMLDFAALLSEKTFSCSCRMQVTTNLMQEFARQVMF
                     FNSKIRILSQKKLRDKLKIYLQTLEIAPDGTVCLPYSRRELSEYLYADRSALSRELCK
                     MRDEGILNFSGSRLQILDPDFLAI"
     gene            852..2048
                     /locus_tag="ACZO9R_03395"
     CDS             852..2048
                     /locus_tag="ACZO9R_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912150.1"
                     /translation="MIKKTKLTAALLTAAMVLQLAACGSNAASGTENTSDNGATAATE
                     TAAASESAESVDTETAATEDTGTHTVVDHAGNTVEVPNHITRVVIDQIPILSTYMSYF
                     GGEAPYIVGYCGSFKDTISKTVLKNIAPELMESADTVYAQSDLNIEEIMKLEPDVILY
                     NAGNTAHAEILQESGIPCIGFATVGHETEADPLNRYKQWLDLLEDVFGEEGKTDAFTA
                     AGDEIVADVEARIAAVPEDERPSALILWKYSDGVPQVSGNGTFGTYWMKHLGVTDVAA
                     EATGFTQVSMEQIYDWNPDILFVDGPGLIGLTSEDVLENRVEGTDFSSIKAVQDGRVY
                     DTTLGMWNWFTPNPDAPLVFAWLACNTYPEVFADYPLEDTIREYYHDWYGYDVTDEEM
                     EEMLQY"
     gene            2048..3076
                     /locus_tag="ACZO9R_03400"
     CDS             2048..3076
                     /locus_tag="ACZO9R_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR0912151.1"
                     /translation="MNDNFLIKRGHFTPLAILLMILLPLVTALICICLGRMNVPVGDV
                     ITVFRDVFTGNEGHAQNYSIVINLRLPRILMAIIVGAGLTCAGNTFQSLFSNPLATPD
                     ILGVTSGTCVGAILAIILSCGILETQLIALAFGLASVFFTLKIAGKNENRQVVYLVLA
                     GVIASSLFNAIGSLLKYTADPQDKLPEITYWLMGSFTSATYKKLLIGSPLILIGIVVI
                     YLLRWRLNILSLSEDEAKASGINLKRTQMIFILASTVITASAVSMCGQVGWIGLLVPH
                     CARMLVGSNNRYVVPISLSLGASFMILIDTLSRTISVIELPLSILTAIIGAPVFISLL
                     NKNRSNLQ"
     gene            3073..3873
                     /locus_tag="ACZO9R_03405"
     CDS             3073..3873
                     /locus_tag="ACZO9R_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912152.1"
                     /translation="MILEIKNGCFGYPKQDIILSDINLSLEEGHILAILGPNGIGKTT
                     LLKCMIGLLPWREGTTFLYGQDIKTLKPKELWSNVSYIPQSHGFAFSYTGLEMVMLGR
                     CSHLGVFEQPGRREIEMAEAMMEKTGITHLAKKDCNRMSGGELQMVLIARALINEPKL
                     IILDEPETGLDFHNQILVLNMIDRLSHEDGISAIMNTHYPTNAMSVADEAFMMNHKGT
                     FFYGSTEKVLTEKNISVSFDVNVVVNEFSHHQKKIKSIVPVSLTDKAM"
     gene            3889..>4427
                     /locus_tag="ACZO9R_03410"
     CDS             3889..>4427
                     /locus_tag="ACZO9R_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR0912153.1"
                     /translation="MSEGVFTIAAKRPIRAEMTISRNTGLGTSNSATWFSLGAGTSIT
                     PESYDTMSIYIGAAGTGTFLIGEQPETMDLHLDEILLVPEKTLCGVKTGEGCIYTEII
                     TKKENTMNDLVKAKEVLNLKKLISYEEGSIANVDIVHNDTMKFVLMAFDEGTGLTPHR
                     APGNAILTALEGTAVIGYEG"
BASE COUNT         1158 a         1107 c         1097 g         1065 t
ORIGIN      
        1 tcaaaaacgg agggcggttg cagcggcaac cggaaatata tgattattga aacagctcta
       61 tatcaggatg tactggaaca ttccagtatc ttccgccaat taccaaaaga taaatatccc
      121 aatgtcctgc gctgcttaaa tgcgcgcgtg gtcgattatc cgcaggatgc atccatcgcc
      181 gcactcgggg attcctccgg ctgctccggc attctgctca ccggcatgct ggaagaatat
      241 ctggtcgacg aaaacggaaa ccagatcatc atcaatcacc tgcgaagcgg cgatgtcttc
      301 ggcgccgagt ctgtgtgcgc cggacaggtg gcgagtcatg tctacattca caccgtggaa
      361 aaaagcaaga tcctgatgct cgattttgcc gcgctcttgt cggaaaaaac attttcctgc
      421 tcctgccgta tgcaggtcac caccaatctg atgcaggaat ttgcgcgcca ggtgatgttt
      481 tttaacagca aaatacggat tctcagtcag aaaaaactgc gggacaagtt aaagatctat
      541 ctgcagacgc tagagatcgc accggacgga acggtgtgcc ttccctacag ccgcagagag
      601 ctttcggaat atctctacgc ggaccggagc gccctctccc gcgaactttg caaaatgcgc
      661 gacgagggga ttctgaattt ttcgggttcc aggctgcaga ttcttgatcc ggattttctg
      721 gcaatataat ctttttttga ggcggttgca atagcaaccg ctttttcttt ccttttccag
      781 tattctatgt ctcgttggtt agttttaact aaccaataat tacatactga taccgttcag
      841 agggaggaaa tatgataaaa aagacaaaac ttaccgctgc attattgaca gctgccatgg
      901 tactgcagct tgcagcctgc ggaagcaatg cggcttccgg aacagagaat acttcagata
      961 acggagccac agcagccacc gagactgctg ccgcttccga atcggcggag agcgtagaca
     1021 ctgaaactgc tgccaccgag gacaccggca cccacactgt ggttgaccat gccggcaaca
     1081 ctgtggaagt tccgaatcac attacccgcg tagtgattga ccagattccg attctctcca
     1141 cctacatgtc ctatttcggc ggggaagctc cttatatcgt aggctactgc ggttctttca
     1201 aggataccat ctcaaagacg gttctcaaaa atatcgcacc ggagctgatg gaatccgcgg
     1261 ataccgtcta tgcacagagt gatctgaata tcgaggaaat catgaagtta gaaccggatg
     1321 tcatcttata caatgccggc aatacggcac acgcagagat cttacaggag agcggcattc
     1381 cgtgtatcgg atttgcaacc gtcggtcacg agacagaagc tgatccgtta aaccgctaca
     1441 aacagtggct ggatcttctg gaggatgtct tcggtgagga aggcaagacc gatgctttca
     1501 ctgctgccgg cgacgagatt gttgcggatg tggaggcaag gattgcagcc gttccggagg
     1561 atgagcgccc gagcgcactg attctgtgga aatacagcga tggcgtaccg caggtatccg
     1621 gcaacggaac ctttggtacc tactggatga aacacttagg tgtcacggac gttgccgcgg
     1681 aggcaaccgg ctttacacag gtgagcatgg aacagatcta cgactggaat ccggatattc
     1741 tgtttgtcga cggtccggga ctcatcggtc ttacctcgga ggacgtgctg gagaacaggg
     1801 tagaaggaac cgatttctcc tccatcaaag ctgttcagga cggacgtgtc tatgacacaa
     1861 cgcttggcat gtggaactgg tttacaccaa acccggacgc tccgctggta ttcgcatggc
     1921 ttgcatgcaa cacttacccg gaagttttcg cagactatcc tctggaggat accatccgtg
     1981 aatattacca tgactggtat ggctatgatg ttaccgatga ggaaatggag gaaatgcttc
     2041 agtactgatg aatgataatt ttttgatcaa acgggggcat tttacaccgc ttgccattct
     2101 cttaatgata ctgctgccgc ttgtcaccgc actgatctgt atctgcctcg gcagaatgaa
     2161 tgtgccggtc ggcgacgtaa tcactgtttt ccgcgatgtt ttcacgggaa atgagggaca
     2221 tgcgcagaat tactccattg taatcaatct gcggctgccg cgtattctga tggcaattat
     2281 tgtgggagca ggtcttacct gcgcaggaaa cacgttccaa tcgctgtttt ccaatccact
     2341 ggcaacaccg gacattctcg gtgttaccag cggcacctgc gtgggagcga tccttgcgat
     2401 catcctttcc tgcggtattc tggagacaca gctcattgca cttgcgttcg gacttgcctc
     2461 cgtatttttt acgttaaaga tcgcaggaaa aaatgagaac cgccaggtgg tctatcttgt
     2521 gctggcaggc gtgatcgcct cctccctgtt taacgccatc ggctctctgc tgaaatatac
     2581 ggccgacccg caggacaagc ttccggaaat tacttactgg ctgatgggca gcttcacaag
     2641 cgccacttat aaaaaattgc tgatcggttc tccgctcatt ctcattggaa tcgtcgtcat
     2701 ctatctgctc cgatggagac tgaatatttt atccttaagc gaggatgaag cgaaagccag
     2761 cggcatcaac ttaaaacgga cacagatgat attcatcctg gcgtcgacag tcattaccgc
     2821 ctcggcggta tccatgtgcg gtcaggtcgg ctggatcggt ctcttagtcc cgcactgcgc
     2881 cagaatgctt gtcggaagca acaaccgtta tgtggttccc atcagcttaa gtctcggtgc
     2941 aagctttatg attctgatcg atacactctc aagaaccatc agcgtcatcg agcttccgct
     3001 gtccattctg accgccatca tcggagcacc ggtctttatt tcactgctga ataagaacag
     3061 gagcaatctg caatgatact tgaaataaaa aatggctgtt ttggatatcc caaacaggat
     3121 ataatattat ctgatatcaa tctctcatta gaggaaggtc atattttagc aatcttaggt
     3181 cccaacggta tcggaaagac cacactatta aaatgcatga tcggtctgct cccctggaga
     3241 gagggaacga cttttctgta cgggcaggac atcaaaacat taaaaccaaa ggaattgtgg
     3301 agcaatgtct cctacattcc gcagagccac ggttttgctt tttcctacac cggactggaa
     3361 atggtcatgc tcggccgctg ttcgcatctt ggtgtttttg aacagcccgg cagacgggag
     3421 attgagatgg cggaagccat gatggagaaa acaggcatca cgcatcttgc caaaaaggac
     3481 tgcaaccgca tgagcggcgg agaacttcag atggtactga ttgcgcgcgc actgatcaat
     3541 gaaccgaagc tgatcattct cgacgagccg gaaacaggtc ttgattttca caatcagatt
     3601 ctggttctca acatgatcga ccggctctcc cacgaggacg gcatcagcgc aatcatgaac
     3661 acgcactatc cgaccaacgc catgtctgtc gccgacgagg cttttatgat gaatcacaaa
     3721 ggcacttttt tctacggcag cacggaaaaa gttctgacag aaaagaatat ttccgtgtcc
     3781 tttgacgtca atgtggttgt caatgagttc agccatcatc agaaaaaaat aaaaagcatc
     3841 gtacctgtct ccctgacaga caaagcgatg tgacagaaag gatctactat gtcagaaggc
     3901 gtatttacca tcgcagcaaa gcgcccgatc cgcgcggaga tgaccatctc acgaaatacc
     3961 ggactcggca cttccaattc cgctacctgg ttttccctgg gcgcaggcac atccatcaca
     4021 ccggaaagct acgatacgat gtccatctat atcggagccg ccggcaccgg aacgtttctc
     4081 atcggtgaac agccggaaac aatggatctg cacctggatg agatattgct cgtaccggaa
     4141 aaaactctct gcggcgtcaa aaccggcgag ggttgtatct atacagaaat tattacaaaa
     4201 aaggagaaca ctatgaacga tcttgttaaa gcaaaagagg tattgaatct gaagaaactg
     4261 atctcttacg aggagggaag tattgcaaac gtggatatcg tacacaacga tacaatgaag
     4321 tttgtcctca tggcattcga tgagggaacc ggactgacac cgcaccgcgc accgggcaac
     4381 gcaatcctga ccgcgcttga gggtacagct gtcatcggat acgaggg
//
LOCUS       DBMDQJ010000115         4427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7460_length_4427_cov_1.417013, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000115 DBMDQJ010000000
VERSION     DBMDQJ010000115.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7460_length_4427_cov_1.417013"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="ACZO9R_03415"
     CDS             <1..341
                     /locus_tag="ACZO9R_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HIR0912154.1"
                     /translation="YDIENSQVVRSQIVDTNGQGHGALAGFLTEAGADVLICGGIGGG
                     AQSALATAGIKLYGGVSGAADEAVEAYLAGKLDYNPDVRCSHHEHEHSCAEHKCGEDK
                     HGCAGNEMLS"
     gene            363..983
                     /locus_tag="ACZO9R_03420"
     CDS             363..983
                     /locus_tag="ACZO9R_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 family protein"
                     /protein_id="HIR0912155.1"
                     /translation="MINREDMLELTRRMTPSRTSFTRVAGGYMEPDGEIDESFNVNFL
                     NLSAAEKEKNLAIAKAVPFAATNVNLRSYAFGDGAEQRKMRQLLYGMRECGLKNDALL
                     YTFYELVGARYRSREPYAILVFHDRYDVPVKASDKERQGESEEVYEYLIGAVAPLVGE
                     YEPGRPVCGFLYPAFADRSTDDAHVAIFQADPEHKIPVFEELFLTR"
     gene            1016..1219
                     /locus_tag="ACZO9R_03425"
     CDS             1016..1219
                     /locus_tag="ACZO9R_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912156.1"
                     /translation="MAGFAIAETAQEYWKEYRKHPVSGCFFCEFEQFYFNTSKHSSEV
                     LDTTEHAFAAFLGKCKENFSRIL"
     gene            1313..2083
                     /locus_tag="ACZO9R_03430"
     CDS             1313..2083
                     /locus_tag="ACZO9R_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR0912157.1"
                     /translation="MNRKILDVTMLCTLCICLMVAANIEEKNTIRESREEISSAYVIS
                     AADKAEVPVAGVCRVLSGYSFTTESDEATGIIKNPRTMVASTAPSDAAEEVQTPLEEA
                     ETPEAKVQAASGEAEAPEAAESKPATAETTTGAENRWNITLTQEEIDLLAKIVWLESQ
                     GEPTEGQEAVVEVVFNRMASEKYPDTLYDVLSQGNPTQFCSWKNRERANPTEKEYTSI
                     HEVLNGNTHILRNDTLYFSTEPLTPRLDQKIGGHSFCY"
     gene            2335..3690
                     /locus_tag="ACZO9R_03435"
     CDS             2335..3690
                     /locus_tag="ACZO9R_03435"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR0912158.1"
                     /translation="MKKRMTILALLLAGAVTLGSYGMVQAGQEKREVPVAGVTQTLAQ
                     VLKEAGGVQAQTADVVEELQSNAAVARMMEPEMVAEEAWETAEIAENELPQTASEFAD
                     IAVAQVNHYVNVRQEPDTESEILGKLYDKSAATVLETTEDGWYRITSGNVNGYVKAEY
                     VVVGDEELARSVGTRLATVTTTTLFVRTEPTTEAKVLTMLPDGDDVVVTDESTEGWAK
                     VSTADGDGYVALDYVTLSTEYIHAESKAEEEARLAREEAERQAAARAAEEARKAREAE
                     AARAAAEQEAARKAAEKQEVKKSGAGSSAGSSSGSSAGSSSGGSAGSGSGSSAGQSAQ
                     TASSNGQAVVDYARQFLGNPYVYGGNSLTNGTDCSGFVKGVYAAFGINLPRTSSEQRS
                     VGYAVSLSEIQPGDIVCYSGHVGIYAGNNTLIHASNEKTGITLTSPVTYRSVLAVRRI
                     F"
     gene            3796..>4427
                     /locus_tag="ACZO9R_03440"
     CDS             3796..>4427
                     /locus_tag="ACZO9R_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR0912159.1"
                     /translation="MRIGGIEAGGTKMVCAIGEVQTDKGQPGENQVTITERVTIPTEK
                     PKITLPRMAAFFEGKGISSLGIGCFGPVDLHRASPTYGYITSTPKLEWQNVDMVGTFT
                     RALGVPVGFDTDVNAAVLGEVTWGAAKDCDTAIYITVGTGVGVGVYCNGALMHGLVHP
                     EAGHLLLQRHPKDTYAGKCPYHANCLEGLASGPAVEARWGRPAKELADRE"
BASE COUNT         1215 a          925 c         1426 g          861 t
ORIGIN      
        1 tatatgatat tgagaatagc caggtggtaa gatcccagat tgtcgacaca aatggacagg
       61 gacacggggc acttgccgga tttttgacgg aggcgggtgc ggatgtgctg atctgtggcg
      121 gaatcggagg cggtgcgcaa agcgcacttg caactgccgg cataaagctg tatggtggag
      181 tgtccggtgc ggcagatgaa gcggtagaag cttatcttgc aggaaaatta gattacaatc
      241 ccgatgtgag atgttctcat catgagcatg agcattcctg cgcggaacat aagtgtggtg
      301 aagacaagca tggatgtgcc ggcaatgaaa tgttatcata aaaacacggg acgggagaaa
      361 ttatgataaa cagagaagat atgctggaac tgacacgcag aatgacgccc agccgtactt
      421 cctttacgcg tgtggcaggc ggctacatgg aaccggacgg agagatcgat gagagcttta
      481 atgtgaactt tctcaatctg tctgcggcgg aaaaggagaa aaatctggcg attgccaaag
      541 cggttccgtt tgcagcaacc aatgtaaacc tgcgcagcta cgcattcggg gatggcgcag
      601 aacagaggaa aatgcgccag ttgttgtacg gaatgcgcga gtgcggactg aaaaatgatg
      661 cactgctgta cacgttttat gagcttgtgg gtgcccggta ccgtagcagg gagccgtatg
      721 cgattctggt ctttcacgac cgctatgatg tgccggtaaa ggcatcggac aaggagcggc
      781 aaggggagtc ggaggaggta tatgaatatc tgatcggtgc agtcgctccg ttggtgggag
      841 agtacgagcc ggggcggccg gtctgcgggt ttttatatcc ggcatttgcc gacaggagca
      901 cagacgatgc gcatgttgcg atctttcagg cggatccgga acacaaaatc ccggtgtttg
      961 aagaactgtt tctcacacgg tagcagagga agattgtgcc ggaaaaggta caaaaatggc
     1021 gggatttgcg atagcagaaa ccgcgcagga atattggaag gaatatagaa aacatcccgt
     1081 ttcgggatgt tttttttgtg agtttgagca gttctatttt aacacatcaa agcattcctc
     1141 ggaagtattg gatacgacag aacacgcttt tgccgcattt ctgggaaaat gtaaagaaaa
     1201 tttttccaga attttgtgaa tgggaaattt gacttttggg aagaatctag tatgatgaaa
     1261 caacgagtgc gagagcagct cacagagtat cattcttgag gaaaggagca aaatgaacag
     1321 aaaaatattg gacgtgacaa tgctgtgcac tttatgcatt tgtctcatgg ttgcggccaa
     1381 cattgaggag aagaacacga tacgggagag cagagaagag atcagcagcg cgtatgtaat
     1441 ttctgcggca gacaaagcag aggttccggt tgcgggtgtg tgccgtgttt tatccggata
     1501 ttcatttacg acagaatcag atgaagcgac aggaatcata aagaacccca gaacaatggt
     1561 agcaagcacg gcgccgtccg atgcggcaga agaagtacag acacccctgg aggaagcaga
     1621 gacaccggaa gccaaagtac aggcagcatc gggggaagca gaggcaccgg aggcggcaga
     1681 gagcaagcct gcgacggcag agacgacgac cggtgcagag aaccgctgga atattacatt
     1741 gacgcaggag gagatagatt tgttggcaaa aatcgtctgg ctggaatcgc agggcgagcc
     1801 gacagaaggg caggaagccg tggtcgaggt agtttttaac cggatggctt ctgaaaaata
     1861 tccagatacc ttgtatgacg tgctcagtca gggaaacccg acgcagttct gttcctggaa
     1921 gaacagagag cgggcgaacc ctacggagaa ggaatacact tcgattcatg aggtattgaa
     1981 tggaaataca catatattaa gaaatgatac gttatatttt tctacggaac cgttgacgcc
     2041 gagattggac caaaagatcg gcggacacag cttctgctat tagtaaggaa gtttgggtta
     2101 aaccggaagg aaaatacgaa gggaacccgc agggacgaaa gaacctttgg gttccttttg
     2161 cgttgcggga caaagcggca gaccggaaaa aaaaaaaaaa aaaatggaaa gagttccctg
     2221 tagattccat gtggttcctt tgttacaata cggttgttgc agaaatgaaa tatttttgta
     2281 acaaataagt aacaatatgg aagcatatgg aataaatcgg gaggattcta cataatgaag
     2341 aaaagaatga caatactggc gcttctgctg gcgggagccg tcacactggg aagctatgga
     2401 atggtgcagg caggacagga aaaacgtgag gtgccggtgg cgggcgtgac acagacgctg
     2461 gcacaggtgc ttaaggaagc gggaggagtg caggcgcaga cggcggatgt tgtggaggaa
     2521 ctgcaaagta acgcggctgt ggcgcggatg atggaaccgg agatggtggc agaggaagcg
     2581 tgggagacgg cggagattgc ggaaaatgaa cttccgcaga ccgcatcgga gtttgcggat
     2641 attgcagtgg cgcaggtgaa tcactatgtc aacgtgcggc aggaaccgga tacggagagt
     2701 gaaattcttg gaaaacttta cgacaaatcc gcagccactg tgctggagac gacggaggac
     2761 gggtggtacc ggattacgtc cggaaatgtg aacggatatg tcaaggcgga atatgtggtc
     2821 gtgggggatg aggagcttgc cagaagtgtg ggaacgcgcc tggcgaccgt cacaacgacg
     2881 accctgttcg tgcggacgga gccgacaacc gaggcgaagg tgcttaccat gctgccggac
     2941 ggcgatgacg ttgtggtaac cgacgagtcg acggaaggct gggcgaaagt gtccaccgcg
     3001 gacggtgacg gttatgtggc actggattat gtgacgctgt ccacagagta cattcatgca
     3061 gagtcaaaag cggaggaaga ggcgcggctt gccagagagg aagcagaacg gcaggcggca
     3121 gcccgtgcgg cggaggaagc gcgcaaagcc agagaggcgg aggcggcacg tgcggcagca
     3181 gaacaggaag cagcccgcaa agcggcagaa aaacaggagg tgaaaaaatc cggcgcgggc
     3241 agcagtgcgg gaagcagttc cggaagcagc gcgggaagca gttccggagg cagcgcggga
     3301 agcggttccg gaagcagtgc ggggcaaagt gcgcagacgg cttcctccaa cggtcaggcg
     3361 gtggtcgatt acgcgagaca gtttctggga aacccttatg tatacggcgg caacagcctg
     3421 acaaacggaa ccgactgctc cggatttgtc aagggcgtgt atgcggcatt tggaatcaat
     3481 ctgccgcgca catcgtcgga acagcggagt gtgggatatg ccgtcagcct gtcggaaatc
     3541 cagccgggcg acattgtctg ctatagcggc cacgtcggca tctatgcggg caataacaca
     3601 ctgattcatg cgagcaatga aaagacaggc attacgctga cttctccggt aacctaccgc
     3661 agcgtgctgg cggtgcggag aattttctaa ttcatccggg agggctgctg tctcttaaat
     3721 attccggttc tttgaaatat gactggcgga gagaactccg aagtggtata tataagaaaa
     3781 gggaggaaga gaaggatgcg gattggtgga atagaagcgg gcggcaccaa gatggtgtgc
     3841 gcgatcgggg aagtgcagac agacaaagga cagccgggag aaaatcaggt cacaatcaca
     3901 gagcgtgtga cgattcccac agagaagccc aagattaccc tgccgcgcat ggctgcgttt
     3961 tttgagggaa aaggaatctc gtcactcgga atcgggtgtt tcggaccggt ggatctgcac
     4021 cgtgcgtccc cgacctatgg atatattacc agcacgccga agctggaatg gcagaatgtc
     4081 gatatggtgg gaacttttac acgggcgctc ggcgtcccgg tcggatttga tacggacgtg
     4141 aatgcggcgg tgcttggcga agttacctgg ggagccgcaa aagactgcga cacggcaatc
     4201 tatattaccg tcggcaccgg cgttggcgtg ggcgtctact gtaacggggc gctcatgcac
     4261 gggctggtgc acccggaggc aggacatctt cttctgcagc gtcacccgaa agacacctac
     4321 gccggaaaat gcccgtacca tgccaactgt ctggaagggc ttgcaagcgg tccggcggtg
     4381 gaggcgcgct ggggcagacc ggcaaaggaa ctggcggacc gggagga
//
LOCUS       DBMDQJ010000116         4427 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7461_length_4427_cov_1.261442, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000116 DBMDQJ010000000
VERSION     DBMDQJ010000116.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7461_length_4427_cov_1.261442"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="ACZO9R_03445"
     CDS             complement(<1..1123)
                     /locus_tag="ACZO9R_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR0912160.1"
                     /translation="MSKFKKLTDFLKSLKCRLILLCILIGIVPGVLLRAGMLGSYENR
                     AVSIRTSEILSQAKILANQIVSNDYLNNAESESAINVQLEQLSTIYDGRVMIIDEEFH
                     IIKDTYNLDTGKTIISEEVIKSIQGEEISKYDSQNRYIEMTIPLVHVDPETENKRTIG
                     VIMVSVSTDSINLNLEYLARNTLVMELIAIVAIIFAGTFIAIHLVAPFHAMAKSIEEI
                     QTGYGDDALKINAYTETRQICEKFNKMLGRMKVLDDSRQEFVSNVSHELKTPLTSMKV
                     LADSINSMEDAPVELYQEFMSDIGNEVDRETKIINDLLSLVKMDKSAGDLNITNVNIN
                     ELLEQILKRLKPIAEKQNIELVLESFRPVSADVDEVKITL"
     gene            complement(1136..1828)
                     /locus_tag="ACZO9R_03450"
     CDS             complement(1136..1828)
                     /locus_tag="ACZO9R_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0912161.1"
                     /translation="MAKKVLVVDDEKLIVKGIRFSLEQDGMEVDCAYDGEEALKMATE
                     NAYDMILLDIMLPKMDGFEVCQHIREFSNMPIVMLTAKGDDMDKILGLEYGADDYITK
                     PFNILEVKARIKAIMRRTAASQPRKEESKVIEAGDLKLDCESRRLFILGREVNLTAKE
                     FDLLELLVNNPNKVYGRENLLNLVWGYEYPGDVRTVDVHVRRLREKIESNPSEPKYVH
                     TKWGVGYYYSQN"
     gene            complement(1829..2638)
                     /locus_tag="ACZO9R_03455"
     CDS             complement(1829..2638)
                     /locus_tag="ACZO9R_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR0912162.1"
                     /translation="MIRTWKKIGAGIILCIFMAGCGSQNEVYLETKVSQETGNPQEPE
                     ETGTGERLDAPEVHGGEQAEETDLPEVSGEEWAAEAALAPETTEDADKADRASGKCYV
                     YVCGEVAEPGVYVLEPGDRIYEAVEMAGGMTADAGTCAVNLAESVYDGLMVYIPDSEE
                     AAGMAGSMTSAGGSAVSAGGSVTSADSSVRNGEGTSGGTASSPEDGRLNLNTASLAEL
                     MTLSGIGQTKAQAVVNYRDAHGGFSSVEEIMNVDGIKEGLYNRIRDQIKVK"
     gene            complement(2725..3912)
                     /locus_tag="ACZO9R_03460"
     CDS             complement(2725..3912)
                     /locus_tag="ACZO9R_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR0912163.1"
                     /translation="MKRLYKRLTAAGLVVVFGMTGVAVMNTAGCGSSGDGVSSTETGQ
                     TAADEAYRSAPADLTLWYADDSYADFFTEAAARYYAKTGKKVAVQYQDTIDYLGTIYD
                     KTMQDDAFPDVYLLPGDNLEEAYLYGLVSVNQADPSENGVLEKAVEAATYEERVLGYP
                     LSYDACVFIYQTDYFTEAPASLQAIIDYSNENEPAENVEYLLEWDVKDAFYDFPFVGN
                     SVTFAKRGSDELDITYDDTLYQQDLEYFDTILASFSIDAEQVSEQHIVNNFREGRTLS
                     AILDTDTLWQLDGYDYGLMQMPELSDTLSAATCASTDMIVVNDYTKDSAAAADFAQFV
                     TIDMADELHGLSGHYAVIPSETPDTVEQTALDAYEGAVLMPDSKDAKNFWVTLEETIL
                     KYF"
     gene            complement(3956..>4427)
                     /locus_tag="ACZO9R_03465"
     CDS             complement(3956..>4427)
                     /locus_tag="ACZO9R_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0912164.1"
                     /translation="GVKPDIMTSAKALGCGVPVGAFLMTERVAEKSLAPGDHGTTYGG
                     NPFVGAAVDTVLKMMERDQITEHVKEITPYLEQKLDALVEKYDFLTARRGLGLMQGVV
                     CEKPVGKIASAALEHGLVVITAGADVLRFVPPLIIGKEHVDEMAEKLDAVLAEM"
BASE COUNT         1001 a         1366 c          884 g         1176 t
ORIGIN      
        1 caagcgtaat ctttacctca tccacatccg cactgaccgg tcggaaactc tccaacacca
       61 gctcgatatt ctgcttttcc gcaatcggct ttaagcgctt taaaatctgc tctaacagtt
      121 cgttgatgtt gacattcgtg atgtttaaat ctcccgcaga cttatccatc tttaccagag
      181 acagcagatc attgatgatc ttcgtctcac ggtccacctc gttgccgata tccgacatga
      241 attcctgata cagctccacc ggtgcgtcct ccatgctgtt gatggaatcc gccagcacct
      301 tcatggaagt cagcggcgtt ttcagctcat gcgagacatt ggacacaaat tcctgtctcg
      361 aatcatccaa caccttcatg cgccccaaca tcttattgaa cttttcacag atctgcctgg
      421 tctcggtgta agcgttaatc tttaatgcgt catccccata tcccgtctga atctcctcga
      481 tcgactttgc catggcatgg aacggcgcca caagatgaat cgcgataaac gttcccgcaa
      541 agatgatcgc cacgatcgca atcagttcca tgacaagcgt attgcgcgcc agatattcca
      601 gattcagatt gatgctgtcg gtcgaaacgc tcaccataat cacaccgatc gtccgcttgt
      661 tctccgtctc cgggtccaca tgcaccaacg gaatcgtcat ttcaatgtaa cggttctggg
      721 aatcatattt gctgatctcc tccccctgga tactcttgat cacttcctcg gaaataatcg
      781 tctttccggt gtcaagattg taggtatcct ttatgatatg gaactcctca tcgataatca
      841 tgacacgccc gtcatagatc gttgacagct gctctaactg cacgttgatc gcagactcac
      901 tctccgcatt attcagataa tcattggaaa cgatctgatt ggcgagaatc ttcgcctggc
      961 ttagaatctc actcgtgcgg atcgacaccg cacggttttc ataagagccg agcatcccgg
     1021 cacgcagcag gacgcccggt acaattccga tcagaatgca gagaagaatc agtctgcact
     1081 taagactttt gagaaaatcg gttaattttt taaatttaga cattgtgtac acccattaat
     1141 tctgtgaata atagtacccg acgccccact ttgtgtgaac gtatttcggt tcgctcggat
     1201 tgctctcgat cttctcccga agacgccgca catgcacatc caccgttctg acatcccccg
     1261 gatactcata gccccataca agatttaaaa gattctctct tccgtatacc ttgttggggt
     1321 tattcacgag cagctctaaa aggtcgaact cctttgcagt gagattgacc tccctgccga
     1381 gaataaacag ccttctgctc tcgcagtcca gctttaagtc tcccgcctcg atcaccttgg
     1441 attcttcttt cctgggctgc gatgcggcag ttcttctcat gatagctttg attctcgcct
     1501 tcacttccag aatattgaac ggctttgtga tatagtcatc tgcgccgtac tccagtccga
     1561 gaatcttatc catgtcatcg cccttggctg taagcatgac aatcggcata ttggaaaact
     1621 cgcggatatg ctggcacacc tcgaatccat ccatcttcgg cagcataata tccagcagaa
     1681 tcatgtcgta tgcattctct gtcgccatct taagcgcctc ctcaccgtcg taggcgcaat
     1741 ccacttccat gccgtcctgc tccagactga aacggattcc ctttacgatt aacttttcat
     1801 catccaccac aagaactttt ttcgccattt atttcacctt tatctgatct ctgatccgat
     1861 tatacaatcc ctctttgatt ccatctacat tcataatctc ttcaacggaa gaaaatcctc
     1921 cgtgcgcatc ccggtagttt acaaccgcct gcgccttggt ctgtccgatc ccggaaagcg
     1981 tcataagctc tgccagcgac gccgtgttga gattcagacg tccgtcttcc ggagacgatg
     2041 ccgtccctcc cgaagttccc tcaccgttcc tgacactgct gtccgcagaa gtcacacttc
     2101 ctcccgcaga aactgcgctg ccgcccgcag aagtcatact gcccgccatc ccggccgctt
     2161 cttcgctgtc aggaatatac accatgagtc cgtcgtatac gctctccgca agattgactg
     2221 cgcacgtccc ggcatccgcc gtcatgccgc ctgccatctc gaccgcctca tagatgcggt
     2281 ctccgggctc taacacgtac acgcccggct ctgccacctc tccgcagaca tagacataac
     2341 attttcccga cgcacggtct gccttatcag catcttccgt cgtctccggt gcaagagctg
     2401 cctcagccgc ccattcctca ccggaaacct ccggcagatc cgtttcttcc gcctgctccc
     2461 cgccgtgaac ctccggcgca tccagacgtt cccccgtccc cgtttcttcc ggctcctgcg
     2521 gattcccagt ttcctgcgag actttcgtct ccagataaac ctcattctga ctgccacatc
     2581 ccgccataaa aatgcacaga attatgccag cccctatctt cttccatgtt cgtatcataa
     2641 gcattctcct tctactgtta aaaggagtcc tcaatgcccg atattcatat tctaacgaaa
     2701 ttttacagcc gtgacaagag tgtttcagaa atatttcaaa attgtttctt ccaatgttac
     2761 ccagaaattt ttggcatcct tggaatccgg cattaaaact gccccctcat acgcgtccaa
     2821 cgccgtctgc tccacggtat ccggcgtctc tgacggaatc acggcgtaat gtcccgacaa
     2881 tccatgcaat tcgtccgcca tgtcgattgt cacaaactgt gcaaaatctg ccgccgctgc
     2941 actgtctttt gtataatcat tgaccactat catatcggta gacgcacatg tcgccgccga
     3001 gagcgtatcg ctcaactccg gcatctgcat cagcccgtag tcatatccgt ccagctgcca
     3061 gagtgtatca gtatccagga ttgccgaaag agtccgccct tcccggaaat tgttcacgat
     3121 atgctgttcg gacacttgct ccgcatcaat ggaaaaagat gccagtatcg tgtcaaaata
     3181 ctccagatcc tgctgataga gcgtatcatc gtaggtgata tccagttcgt ccgatcctct
     3241 ctttgcgaac gtgacgctgt tgccgacaaa cgggaaatca tagaacgcat ccttgacatc
     3301 ccactccagc agatattcca cattctctgc cggctcgttc tcattggaat aatcgatgat
     3361 tgcctgcagc gatgccggcg cctcggtaaa ataatccgtc tggtaaataa acacgcacgc
     3421 atcgtaggac agcggatacc cgagcacgcg ctcttcgtag gtcgccgcct cgactgcctt
     3481 ttccagcacg ccgttttccg acgggtccgc ctgattcacg gaaacgagac catagaggta
     3541 tgcttcctcc agattgtccc caggtaagag gtaaacatcc ggaaatgcat catcctgcat
     3601 ggtcttatcg taaatagttc caagataatc aatggtatcc tgatactgca cggctacttt
     3661 tttccctgtc ttggcgtaat agcgcgcagc cgcctcggta aaaaagtctg catagctgtc
     3721 atccgcatac cacagcgtaa ggtctgccgg tgcgctccgg taagcttcat ccgccgccgt
     3781 ttgtcccgtc tccgtggatg acacaccatc cccgctgctg ccgcaacctg ctgtattcat
     3841 aacagccact cctgtcattc caaacacaac gacaagtcct gccgccgtca gtcttttgta
     3901 taatcgtttc attttatttc cctaatccgt tttttgtatt ctcattatca gggaattaca
     3961 tctctgccag aaccgcatcc agcttctctg ccatctcatc cacatgttct ttcccgataa
     4021 tcagtggagg tacaaaacga agcacatcgg cgcctgccgt gatcacgaca agtccgtgtt
     4081 ccaatgctgc tgacgcaatt tttccaaccg gtttttcgca cacaacgccc tgcatcagcc
     4141 ccagacctct gcgcgccgtg agaaaatcat acttttccac aagcgcatcc agcttctgct
     4201 ccagataagg tgtgatctct tttacatgct ctgtaatctg atcccgctcc atcatcttaa
     4261 gcacggtgtc aactgctgcc ccgacaaacg ggttgccgcc gtaggtcgtc ccgtgatcac
     4321 ccggcgccag cgatttttcc gccacgcgct cggtcatcag gaatgctccc accggaacgc
     4381 cgcatccaag agccttcgcg ctggtcataa tgtccggttt cacgccg
//
LOCUS       DBMDQJ010000117         4391 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7529_length_4391_cov_1.458741, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000117 DBMDQJ010000000
VERSION     DBMDQJ010000117.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4391)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4391)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4391
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7529_length_4391_cov_1.458741"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..229)
                     /gene="larC"
                     /locus_tag="ACZO9R_03470"
     CDS             complement(<1..229)
                     /gene="larC"
                     /locus_tag="ACZO9R_03470"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HIR0912165.1"
                     /translation="MKTIYLECNMGAAGDMLMSALYEICDQKEWFLHKMNTVFAPYHI
                     VLSPESAVSCGINGTHMKVTVDGTEEVSLDVK"
     gene            complement(270..1022)
                     /gene="larB"
                     /locus_tag="ACZO9R_03475"
     CDS             complement(270..1022)
                     /gene="larB"
                     /locus_tag="ACZO9R_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR0912166.1"
                     /translation="MENDHIRTILNNIENGTLTASEAYAQLKLAPFEDLGYAKVDHHR
                     EVRQGAPEVIYGASKTKEQIAGITNSLLAHCPENILITRLSPESAAYLSERFTLFYDE
                     ASRIGIVNRTPHITAEGTIVVATGGTSDIPVAEEAALTAEVLGNKVVRVYDVGVAGLH
                     RLLSNMELLSSANAIVAVAGMEGALASVIGGLVDCPVIGVPTSVGYGASFHGISALLS
                     MLNSCASGVSVVNIDNGFGAGYLASMINHIGG"
     gene            1308..>1396
                     /locus_tag="ACZO9R_03480"
     CDS             1308..>1396
                     /locus_tag="ACZO9R_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012655.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR0912167.1"
                     /translation="MDDVAKILGMSKKTIYTVFRDKESMFLAMV"
     assembly_gap    1397..1406
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1407..1806
                     /locus_tag="ACZO9R_03485"
     CDS             <1407..1806
                     /locus_tag="ACZO9R_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR0912168.1"
                     /translation="SIKESEQQIVEDDALSTVEKIRRILGVIPESYRDVDFRQLYLLK
                     DKYPVIYKQVEKRLETGWETTIQLLEQGMAEGVVRKVSIPIVKMMLEATVEQFFQRDI
                     LIQSRLSYLEALDEVVSILVNGIVVPEAEG"
     gene            1847..4252
                     /locus_tag="ACZO9R_03490"
     CDS             1847..4252
                     /locus_tag="ACZO9R_03490"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR0912169.1"
                     /translation="MENRVYLNDDWKFSEQFTEEMLGDGFDVGNMTPVRLPHTVKETP
                     LHYFDESVYQMVSGYRRDVFISEEWSGKRLLLTIDGAAHESEVYWNGKKIGEHHCGYT
                     AFTMDVSDDAIYGIRNTLVVKVDSRESVNIPPFGFVIDYMTYGGLYREVWLDIKEKTY
                     LKDVFVRGDLSGDTGRLISEITADGGGENLSVRQKIRRCGESGYRLLGECELTGTKLV
                     LDYTVESVLPWDTVHPVCYEVCTQLIRDEKVLDENVTVTGFRHAEFKADGFYLNGKKV
                     KLRGLNRHQSYPYVGYAMPESMQRMDADILKYELGVNAVRTSHYPQSHYFIDQCDRIG
                     LLVFMEIPGWQHIGDEAWKDQAVINVRDMVMQYRNHTSIILWGVRINESQDDDDFYRR
                     TNAAARELDPSRPTGGVRAHKKSSLLEDVYTYNDFVHDGTNQGCEKKAAVTSDPKKPY
                     LISEYNGHMYPTKAFDWEEHRAEHALRHVRVLDAAAAEGDIAGSFGWCMFDYNTHKDF
                     GSGDRICYHGVMDMFRNPKLAAKVYECQQEEHTVLELSSSMDIGEHPGCNRGETYIFT
                     NADSVRMYKNDRFIKEYKREDSPWKHLPHGPLVIDDYIGDAIEKGEHFTPAQGKGIKD
                     ALNATARYGLSHLPKSVYVTALKMLLLYHMKPTDAVVLYNRYIGDWGGTSTTYRFEAV
                     SDGGVTAELIKKPMTKVVLFARADHTGLQEKSTYDVAAIRIEARDEYGNLLSFFNEPV
                     SFEVSGPVELIGPSVISLEGGMGGTYIKTTGECGEARLVIRNAQTEPVELHFAAAAER
                     QRL"
BASE COUNT         1138 a          985 c         1319 g          939 t
ORIGIN      
        1 ccttgacatc gagagagacc tcctcggtac cgtccaccgt tactttcatg tgggttccgt
       61 tgataccgca gcttacggcg gattccggag aaagcacgat atgataaggg gcaaagacgg
      121 tgttcatctt gtgcaaaaac cattcttttt gatcgcagat ctcataaagg gcactcatca
      181 gcatatctcc ggcagcaccc atattacatt ccagataaat tgttttcata gtgagaagaa
      241 acctcctgtt agagtgatgg atggtgtgat cagccgccga tgtggttgat catgcttgca
      301 aggtagcccg cgccgaagcc gttgtcgatg ttgacgacag atacgccgct ggcgcaggaa
      361 ttgagcatgg acaatagcgc ggaaatgccg tgaaagctgg cgccgtaccc aacgcttgtg
      421 ggaacgccga tgacagggca gtccacgaga ccgccgatga cgctggcgag agcgccctcc
      481 atgcctgcga cggcgacgat ggcattggcg gatgagagca gctccatgtt ggagagcagg
      541 cggtgcagtc ccgcgacgcc gacgtcatag acgcgtacga ccttgttgcc gagcacttct
      601 gccgttaagg ctgcctcctc cgcgacggga atgtcgctcg tgccgcctgt ggctacgacg
      661 atggttccct ccgctgtgat atgcggcgta cggttcacaa tgccgatgcg ggacgcctca
      721 tcgtagaaca gcgtaaaacg ctcggagagg taagcagcgg attccgggga aagcctcgtg
      781 attaagatat tctccggaca gtgggcgagc agactgttcg tgatgcccgc gatctgttct
      841 ttggtcttgg aggctccgta gatcacttcc ggcgctccct gacgcacttc gcggtgatga
      901 tcgaccttgg cgtacccgag atcctcaaaa ggagccagct ttaactgcgc gtaggcttcg
      961 ctcgcggtga gcgttccgtt ttcaatatta ttaagaatcg ttctgatatg atcgttttcc
     1021 atgatgtcct ccatgtctgt aattaaccat attataccca ttgtacggcg ggattgcaag
     1081 aacgcgcagg cgcttgaaag cccggaattt agtacattta tacaaaaaat ggaaactgat
     1141 tttgtgaatt cggtttcttg tgagaacctg gaaatcgtga tagaataaag gaaaccagaa
     1201 agataaaaat agtttccaaa agagggaggt ggctaaagat gcagcagaaa gaagtgatcc
     1261 tggagggaac gatcaaagcg tttaatgaga aaggcctgaa gttcactatg gacgatgtgg
     1321 cgaaaatcct cggaatgagc aaaaagacca tatataccgt attccgcgac aaggagtcga
     1381 tgtttcttgc gatggtnnnn nnnnnnttcc atcaaggaga gtgaacagca gattgtggag
     1441 gatgacgcgc tctctacggt ggagaagata agacggattc tcggagtgat tccggagagt
     1501 taccgggatg tggatttcag acagctctat ctgttaaagg acaagtatcc ggtgatctac
     1561 aagcaggtgg aaaaaagact ggagaccggc tgggagacaa cgatccagct tctggagcag
     1621 ggaatggcgg aaggcgtggt ccgcaaggtc agcattccga tcgtaaagat gatgctggag
     1681 gccacggtag aacagttttt ccagagagat atcctgattc agagcaggct ttcctatctg
     1741 gaagcgctcg atgaggtggt ttcgattctg gtaaacggaa tcgtggtgcc ggaggcggag
     1801 ggataaacgc gtggcaatgc aggaaggtgg caggcgaagg tagaacatgg agaacagagt
     1861 atatttaaat gacgactgga agttttcgga acaatttacg gaagaaatgc tgggcgatgg
     1921 cttcgacgtg ggaaatatga ctccggtacg gcttccgcat acggtaaagg agacaccgct
     1981 tcactatttt gatgaatctg tttaccagat ggtcagcgga taccgtagag atgttttcat
     2041 ttcagaagaa tggagcggaa aacgtctgct gctcacgatt gacggggcgg cgcatgagag
     2101 cgaagtctac tggaacggga agaagatcgg ggaacatcac tgcggctaca cggcattcac
     2161 gatggatgtc agtgacgatg ccatctacgg catccgcaac acgctggtgg tcaaggtgga
     2221 cagcagggag agcgtgaata taccgccgtt tggatttgtg attgattata tgacgtacgg
     2281 cggactctac cgtgaggtgt ggctcgacat caaggagaag acctatctga aagatgtgtt
     2341 tgtgcgggga gatctttccg gtgacaccgg cagactcatc tccgagatta cggcggacgg
     2401 cggcggggag aatcttagcg tcagacagaa gatcagaaga tgcggcgaga gcggctaccg
     2461 tcttctgggc gaatgtgaac tgaccggaac aaagcttgtg ctcgactata cggtggaatc
     2521 cgttctgccg tgggacaccg ttcatccggt ctgctatgag gtgtgcacac agctcatccg
     2581 ggatgaaaag gtgctggatg aaaatgtgac ggtcacaggc tttcgacatg ccgagtttaa
     2641 ggcggacggt ttttatctga acggtaaaaa agtaaagctc cgcggactca accgacatca
     2701 gagctatccg tatgtgggat atgccatgcc ggaatccatg cagcggatgg acgcggatat
     2761 tttaaagtat gagcttggcg tcaacgccgt gcggacatcc cactatccgc agtcccatta
     2821 ctttatcgac cagtgtgaca gaatcggact tctggtattt atggagattc cgggatggca
     2881 gcacatcggc gatgaggcgt ggaaggatca ggcggtaata aatgtgcgcg atatggtgat
     2941 gcagtaccgc aatcacacgt ccatcattct ctggggtgtg cgcatcaacg agtcgcagga
     3001 cgatgatgat ttttaccgca ggacgaacgc ggcggcgcgc gaactcgatc catccagacc
     3061 gacgggcggt gtgcgggcgc acaaaaagag cagtcttctg gaagacgtct acacttacaa
     3121 tgattttgtg catgacggca cgaaccaggg atgtgagaaa aaggcggcgg tgacatccga
     3181 tccgaaaaag ccgtatctga tcagcgagta caatggccac atgtatccga ccaaggcgtt
     3241 cgactgggag gaacaccgcg cggaacacgc gctgcgccat gtgcgggtgc tcgacgcggc
     3301 ggcagcggag ggtgacattg caggaagctt cggctggtgc atgttcgact acaacacaca
     3361 caaggatttt ggcagcgggg accgcatctg ttaccacggc gtgatggata tgttccgcaa
     3421 tccgaagctg gcggcgaagg tctatgagtg ccagcaggag gaacatacgg ttctggaact
     3481 gagttcctcg atggacattg gggaacaccc gggctgcaac cgcggcgaga cctacatttt
     3541 taccaacgcg gacagtgtgc ggatgtacaa aaacgaccgt tttatcaagg aatacaagag
     3601 agaggattcc ccgtggaaac atctgccgca cggaccgctc gtgatcgacg attatatcgg
     3661 agatgcgatc gagaagggcg aacattttac accggcgcag ggaaagggaa tcaaggatgc
     3721 gttaaatgcg accgcacgct acggactgtc acatcttccg aaatccgtgt atgtgacggc
     3781 gcttaagatg ctgctccttt atcacatgaa gccgaccgat gcggtggtgc tctataaccg
     3841 ctatatcgga gactggggag gcacttccac gacataccgg tttgaggcgg tgtcggacgg
     3901 cggcgtgact gcggaactca tcaaaaagcc gatgacaaag gttgtacttt ttgcgcgggc
     3961 ggatcatacg ggacttcagg agaaaagcac ctacgatgtg gcggccatcc ggattgaggc
     4021 gcgggacgaa tacggcaatc tgctaagctt ctttaatgag ccggtaagct ttgaggtgag
     4081 cgggccagtg gagctgatcg gaccgtctgt catctcgctg gagggaggaa tgggcggcac
     4141 ctacataaag acaacgggtg agtgcggcga ggcgcggctt gtcatccgga atgcacagac
     4201 cgaacctgtg gaactgcact ttgcggcagc ggcagagcga cagagattgt aagaaaagaa
     4261 agggaacaca ggcaatggaa gaaaaatcaa aagttatttt tggcaatccg atgccggata
     4321 aggtataccg gaaaacggta aagtcgaaaa agaaatatga gataaattac ttatcagagg
     4381 atttttttga a
//
LOCUS       DBMDQJ010000118         4386 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7536_length_4386_cov_1.403734, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000118 DBMDQJ010000000
VERSION     DBMDQJ010000118.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4386)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7536_length_4386_cov_1.403734"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(80..1336)
                     /locus_tag="ACZO9R_03495"
     CDS             complement(80..1336)
                     /locus_tag="ACZO9R_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912170.1"
                     /translation="MEKIKKFWNAVSYEVVIAAGLIVWFLTGRALDLQGWSSAWNAMD
                     YSMGTGSRLLIGSIYRLFYGEYLDYTVAYKYVAVGTMLTILVLAIVLGRMIRLAVAKN
                     DQIRHAVFGAVALYLVAPFSIAYVWNDQNLGRLDVYMLLVALLALFVGLTIKNTYVKC
                     VCYTVLGVVGLAIHQGFAFLYYPIVFSVMVYDVFEVNRVHAGRFAAAFVSGLINVAVA
                     VYFQFFSSIHFDSAKELVTFLEGRTNLTISEYALDIEYFLKMDYQLKEITPQFYKYDA
                     PIRHALLVLLLLLPVLVWCFQIYRDVFGWQKKSNVKWYASPYFYMLLSQLCFVPMFVI
                     HTDWGRHFAPLFAMQIFMFLFYAAKGEPAMVYACENMCGRMKKHPWIFVVSILWIAKL
                     DSFGARDFLKQAMYLFDFLQRGFFIN"
     gene            complement(1341..2738)
                     /locus_tag="ACZO9R_03500"
     CDS             complement(1341..2738)
                     /locus_tag="ACZO9R_03500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019161136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HIR0912171.1"
                     /translation="MEKSMDKIVALAKSRGFVYPGSEIYGGLANTWDYGNLGVELKNN
                     VKRAWWQKFIQENPYNVGVDCAILMNPQTWVASGHLGGFSDPLMDCKECHERFRADKI
                     IEDYAEEKGITLQSSVDGWTQEEMKNFIEENQVPCPSCGKHNFTDIRQFNLMFKTFQG
                     VTEDAKNTVYLRPETAQGIFVNFKNVQRTSRKKIPFGIGQVGKSFRNEITPGNFTFRT
                     REFEQMELEFFCEPGTDLEWFQYWRGFCRDWLISLGIKEDEMRLRDHDPAELAFYSKG
                     TTDIEFLFPFGWGELWGIADRTDYDLGRHQETSGQDLTYFDDEKNERYLPYVIEPSLG
                     ADRVVLAFLCAAYDEENIGTEEKPDMRTVLHFHPALAPVKVGVLPLSKKLNEGAEKIF
                     AQLSKKYNCEFDDRGNIGKRYRRQDEIGTPFCVTYDFESENDGAVTVRDRDTMEQERI
                     KIEELDAYFAQKFAW"
     gene            complement(2779..3549)
                     /locus_tag="ACZO9R_03505"
     CDS             complement(2779..3549)
                     /locus_tag="ACZO9R_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912172.1"
                     /translation="MKRLVCTVCSVLLCVGLLSGCGTSLEGEESVVYVGKKGVIESLD
                     VESLDQSYYDETELKSYVDAEVKDYTAEHGKNAVEVESLKVEDGVAKLKMKYKTPEDY
                     TAFNGIELYQGKVVASLAAGYVYDGEFARVEEGKVVGAATKQDIYSEDDLKVAIIRAN
                     TDVKVDGEICYVSCQNVKLTGTDSVSIRDGYYLETGSVTAAVDVTGQENTGTEQLPGT
                     EQAEMTGETVHTDDTEQTETASGDGSFETDVYTFIVYK"
     gene            complement(3594..4361)
                     /gene="recO"
                     /locus_tag="ACZO9R_03510"
     CDS             complement(3594..4361)
                     /gene="recO"
                     /locus_tag="ACZO9R_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR0912173.1"
                     /translation="MGNNIVVTGMVLQVMPIGEYDKRITLLTKERGKITAFARGARRP
                     NSQLLAAANPFCFGEFELFEGRSAYTLARATIQNYFRELTGDFDATYYGFYFLEFADY
                     YCQENNDEREMLKLLYQSLRALASSAYDNRLVRAVFELKALAVNGEAPNVFSCLSCGS
                     REHLTWFSVKRGGILCAECRNEAPDAVELNESTVYTMQYVILARIEKLYTFSVSGQVL
                     EELTRLLRSYFGVYVGHAFKSLKILEDAEKVEIMFLK"
BASE COUNT         1102 a         1314 c          896 g         1074 t
ORIGIN      
        1 atgttacgtc tgtggaaagt caaaacgtaa aaaggcagaa ccgcaatctc cggttccgcc
       61 cttctttttc cagtccgatt cagttgataa aaaacccacg ctgcaggaaa tcaaacagat
      121 acattgcctg tttcaagaaa tctctagccc cgaagctgtc gagctttgcg atccagagga
      181 tcgacacgac aaagatccag ggatgcttct tcatcctgcc gcacatattc tcacacgcgt
      241 aaaccatcgc cggctctccc ttcgccgcat agaacagaaa cataaaaatc tgcatggcaa
      301 agagcggtgc aaaatgtctg ccccagtccg tgtggatcac gaacatcggc acaaaacaca
      361 gctggcttag cagcatgtaa aaatacggcg atgcatacca cttcacattg ctctttttct
      421 gccagccaaa gacatcccgg taaatctgaa aacaccacac aagcaccggc agcaacaaca
      481 gaagaaccaa cagcgcatgc cggatcggcg catcatattt gtaaaactgg ggtgtaatct
      541 ccttcagctg gtaatccatt ttcagaaaat actcaatatc aagcgcatat tccgaaatag
      601 tgaggttcgt tctcccctcc agaaatgtca caagttcctt cgcactgtca aaatggatac
      661 tgctgaaaaa ctgaaaatac accgctaccg ccacattgat gaggccggat acaaacgcgg
      721 cggcaaacct gccggcatgc accctgttta cctcaaacac gtcatatacc atcaccgaaa
      781 agacaatcgg ataatacaaa aatgcaaatc cctggtgaat ggcaagtccc acgacaccga
      841 gcacggtata gcagacacac ttcacatatg tattcttaat cgtaagacca acaaacagtg
      901 ccagcagtgc aaccaacagc atgtagacat ccagtctgcc taaattctga tcgttccaca
      961 cataggcaat cgaaaacggt gcgacaaggt acagcgccac cgccccaaag acggcgtgcc
     1021 ggatctgatc gttcttagcg acggcaagac ggatcattct ccccagcaca atggcaagca
     1081 ccagtatcgt gagcatcgtt cccacggcga cgtatttgta tgccaccgtg taatccagat
     1141 actctccgta aaacaggcgg taaatgctcc cgatgagaag ccttgatccc gttcccatgg
     1201 aatagtccat cgcattccat gccgatgacc agccctgcag gtctaacgcc ctgcctgtca
     1261 gaaaccagac gatcaggccg gctgcaatca caacctcata cgatacggca ttccaaaact
     1321 tttttatctt ctccatctgc ctaccacgca aacttctgtg caaagtaagc atccagttcc
     1381 tcgatcttga tgcgctcctg ctccatcgta tcacggtcac gcacggtcac agcaccgtcg
     1441 ttctcagact caaaatcgta tgttacgcag aacggagttc cgatctcatc ctgtcttctg
     1501 tatctcttac cgatattgcc tctgtcgtcg aattcacagt tatatttctt ggagagctgt
     1561 gcgaaaatct tctctgcgcc ctcgtttaac ttcttggaaa gcggaagcac tccgacctta
     1621 accggagcca gtgccggatg gaaatgaagc acggttctca tatccggctt ctcctcggta
     1681 ccgatgttct cctcatcgta agccgcgcat aagaatgcca gtaccacgcg gtctgctccc
     1741 agagacggct caatgacgta cggcagataa cgctcattct tctcgtcgtc aaagtaggta
     1801 agatcctgac cggacgtctc ctgatgtctg ccgagatcat agtcggtacg gtctgcgatg
     1861 ccccagagtt caccccagcc aaacgggaat aagaactcga tatccgtcgt tcccttgctg
     1921 tagaatgcaa gttctgccgg atcgtgatcg cgcagacgca tctcgtcttc cttaatgccg
     1981 agagagatca gccagtcacg gcagaagcct ctccaatact ggaaccactc cagatcggtt
     2041 cccggctcgc agaagaactc cagctccatc tgctcaaact cacgggtacg gaatgtaaag
     2101 ttacccggcg taatctcgtt acggaacgac tttccaacct ggccgattcc aaacgggatc
     2161 ttcttgcggg aagttctctg aacattcttg aagtttacaa aaataccctg tgcggtctcc
     2221 ggtcttaaat aaaccgtatt cttcgcatcc tcggtcacgc cctggaatgt cttaaacatc
     2281 aagttgaact ggcggatgtc tgtaaaattg tgcttgccac agctcgggca cggaacctgg
     2341 ttctcctcga tgaaattctt catctcctcc tgtgtccatc cgtctacgga gctctgtaag
     2401 gtgatgccct tctcctccgc gtagtcctcg ataatcttat ccgcgcggaa acgctcatgg
     2461 cactccttac agtccatcag cggatccgag aaaccgccga gatgtccgga agccacccac
     2521 gtctgcggat tcatcagaat cgcacagtcc acaccgacat tgtacggatt ctcctgaata
     2581 aatttctgcc accatgctct cttgacgtta ttcttcagtt cgacgcccag attgccgtag
     2641 tcccatgtat ttgcaagtcc gccgtaaatc tccgatcccg gatatacgaa acctcttgat
     2701 tttgccagcg ccacgatttt gtccattgat ttttccattc taaattcctc gcctttctta
     2761 ggttatattg tgatatgttt atttgtaaac aatgaaagta tatacgtctg tctcgaacga
     2821 gccgtcgccg gacgccgtct ctgtctgctc ggtatcatcc gtatgcaccg tctccccggt
     2881 catttccgcc tgttcggttc ccggaagctg ctcggtgccg gtgttctcct gcccggtcac
     2941 atccacagct gccgtcacac ttccggtttc aagataatat ccgtcacgga tcgatacgct
     3001 gtcggttcca gtcagcttca cattctgaca ggagacatag cagatctcac cgtccacttt
     3061 cacatctgta ttggcacgga taatagccac tttcagatca tcctcagagt aaatatcctg
     3121 ctttgtggca gctcccacaa ccttgccttc ctccacgcgg gcgaactccc cgtcatagac
     3181 gtatcctgcc gccagagagg caacgacttt cccctgatag agttcaattc cgttaaacgc
     3241 ggtataatcc tccggtgtct tgtacttcat cttaagcttc gccacaccgt cttccacctt
     3301 aaggctctcc acctcgactg catttttgcc atgctccgcg gtgtaatctt tcacctctgc
     3361 atccacatag gacttcagtt ccgtctcatc gtagtaggac tgatcgagcg actccacatc
     3421 cagcgactct atcacgcctt tctttcccac gtacacgaca ctttcctctc cctccagcga
     3481 ggtaccgcat ccggagagaa gtcccacaca caacagtaca ctgcagaccg tgcacactaa
     3541 tctcttcatc acataatctc cattctttct gaaaaagtat cggtgatatt atactatttt
     3601 aaaaacatga tttcaacctt ttccgcatcc tcaagtatct ttaacgactt gaaggcgtgt
     3661 cccacgtaca cgccaaaata gctgcgcaaa agtctggtca gttcttccag cacctgcccg
     3721 ctcacggaaa acgtatataa tttttcaatc cgcgccaaga tcacgtactg catggtatag
     3781 acggtggatt cattcaattc caccgcatct ggcgcctcat tgcggcattc tgcacagaga
     3841 atcccgccgc gcttgacgga aaaccaggtc agatgctccc tgctgccgca ggaaagacag
     3901 gaaaacacat tcggagcctc gccgtttacc gccagcgcct tcagttcaaa caccgcgcgc
     3961 accagccggt tgtcgtaggc actgctcgca agggcgcgca gcgactggta caaaagcttt
     4021 agcatctcgc gctcatcgtt attttcctga caataataat ccgcaaattc cagaaaatag
     4081 aacccgtaat atgtagcatc aaaatccccg gtcagctccc ggaaatagtt ctgaatcgtc
     4141 gctctggcaa gcgtgtaagc acttcttccc tcgaacagct cgaactctcc gaagcaaaac
     4201 ggattggcag ctgcgagaag ctggctgttc ggccgcctcg ctccccgcgc aaatgctgtg
     4261 atcttccctc tttcttttgt cagcagtgta atccgcttgt catattcgcc aatcggcatt
     4321 acctgtaaga ccatcccggt caccacaata ttgtttccca tccggctgct cttcccccgc
     4381 caaaat
//
LOCUS       DBMDQJ010000119         4375 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7556_length_4375_cov_1.790594, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000119 DBMDQJ010000000
VERSION     DBMDQJ010000119.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7556_length_4375_cov_1.790594"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="ACZO9R_03515"
     CDS             <1..559
                     /locus_tag="ACZO9R_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR0912174.1"
                     /translation="VILTAYTMSRFTFAARKPMMKITMVLGMFPSFMGMIAVYLLMTQ
                     FNLINHLWGLILIYSAGAPMGYLTQKGFFDTIPKAIDEAARIDGATNFQVFMKINLPL
                     SRPIIVYTALSSFTWPWSDFILPKLLLKEKNLYTVAVGLMSLDETEFARFAAGSVFIA
                     VPIVLLYFFLVKDMVNGMAAGAVKG"
     gene            671..1291
                     /locus_tag="ACZO9R_03520"
     CDS             671..1291
                     /locus_tag="ACZO9R_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HIR0912175.1"
                     /translation="MQTVLLIVALSMDVFLASVACGAEQIRIEKKTALCIGAVCSGVL
                     FFSLVCGKLIDGVVREKYAAYLCFAGLFLVGAYKLTEYGIRAYIRRHHFLCKQVKMKF
                     SQLNFIISIYNNPAAADRDCSATMSVTEGICFALAMSVDGFFGGLGAAFLDISIAGTV
                     CLNLVFSALAVLGGSAVGKYAADRCKRDYSWIGGALFVVLAFSKIL"
     gene            1581..3095
                     /gene="glpK"
                     /locus_tag="ACZO9R_03525"
     CDS             1581..3095
                     /gene="glpK"
                     /locus_tag="ACZO9R_03525"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HIR0912176.1"
                     /translation="MAKYVMALDAGTTSNRCILFNEQGEICSMAQKEFRQFFPNPGWV
                     EHDANEIWSTMLGVAVEAMNMIRATAADIAAIGITNQRETAIVWNKETGEPVYNAIVW
                     QCRRTSDIADALKAKGLEETYRQKTGLIIDAYFSATKIKWILDNVEGARELAEQGKLL
                     FGTVETWLIWKFTKGAVHVTDYSNASRTMLFNINDLCWDKEILEELEIPECMLPTPRP
                     SSEVYGYTDPSFLGDAIPIAGAAGDQQAALFGQTCFHEGEAKNTYGTGCFLLMNTGEK
                     PVFSKNGLVTTIAWGLDGKVNYALEGSIFVAGAAIQWLRDNMKMIDSSMDSEYMAKKV
                     HGTHGCYVVPAFTGLGAPHWDQYARGTIVGLTRGTNKNHIIRATLESIALQVCDVIDA
                     MRADAGIDLKSLKVDGGASANNFLMQFQADMINAPVKRPQCVETTAMGAAYLAGLAVG
                     YWKDKEDVVNNQKIDRIFTPEMEEEERKAKRKGWNKAVKYAYGWAKDEPEEEEE"
     gene            3318..4073
                     /locus_tag="ACZO9R_03530"
     CDS             3318..4073
                     /locus_tag="ACZO9R_03530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0912177.1"
                     /translation="MEIRLLYGNEIQWAVQTAHEVYEQCVRPYTRNAAEVQQYYDYMN
                     LPNLSQEMLAGRLFLWGAFENGQMCGVSAMQNVGHITALYVRPQYFRRRIGSNLVDRM
                     CNFAASRLQCGRVTVQAAPSAANYFYHTGFTLIQGAPAGMYVPLERRIWSVPMTQGYM
                     GNIGGSGSTSTQGGGVPVAQPRRTKPEITYPKKKVSARVVLWLTAVTIAFAVAVVTGI
                     TVYHITQEGITGGYEMPDPVGDENTETDDGQEL"
     gene            4136..>4375
                     /locus_tag="ACZO9R_03535"
     CDS             4136..>4375
                     /locus_tag="ACZO9R_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HIR0912178.1"
                     /translation="MDTTTKGTRTKYRLAEAMKECMKTTPVDNITVTQLTELCGVTRQ
                     TFYRNFIDKYDLINWYFDILLHKSFEHMGSGKNIYE"
BASE COUNT         1103 a          885 c         1376 g         1011 t
ORIGIN      
        1 ggtgatcctg actgcctata ccatgtcgcg ttttacgttc gcggcgcgca aaccgatgat
       61 gaagattacg atggtgcttg gaatgtttcc ttcctttatg ggaatgattg cggtgtacct
      121 tctgatgacg cagtttaatc tgatcaatca tctctgggga ctgatcttaa tctacagtgc
      181 gggagcgccg atgggctatc tgacgcagaa aggctttttc gatacgattc ccaaggcgat
      241 cgacgaggcg gcgagaattg acggagccac gaattttcag gtttttatga aaatcaatct
      301 gccgctctcc cggccgatca tcgtctatac tgcgctctct tcttttacct ggccgtggag
      361 cgactttatt ctgccgaagc ttttgctgaa ggaaaagaac ctgtatacgg tggcggttgg
      421 tctgatgagc ctcgatgaga cggaatttgc aagatttgcg gcgggaagcg tgttcatcgc
      481 ggtgccgatt gtcctgcttt attttttcct ggtcaaggac atggtgaacg gcatggctgc
      541 gggcgcggtg aaagggtgac ccggaataca aagtggcggc gggcgcggta aaagggtgat
      601 ccggaataca aagtggcagc agccgcggat ttgaataaga tagaatgaga aagccgtttc
      661 gaggaaaaat gtgcagacag ttttattgat cgtagcgctc tctatggacg tctttctggc
      721 atccgtggcg tgcggggcgg aacagatccg gatcgaaaag aagacagcac tctgcatcgg
      781 cgcggtatgc agcggagtgc tgtttttttc attagtgtgc ggaaaactga tcgatggagt
      841 ggtcagggaa aaatatgcgg cgtatctgtg ttttgcggga ttgtttctgg tcggcgctta
      901 taagctgacg gagtacggaa tccgggcgta tatcaggaga caccatttcc tgtgcaagca
      961 ggtaaaaatg aaattttcac agctgaattt tatcatcagc atttacaata atccggcggc
     1021 ggcagatagg gattgctctg cgaccatgtc agtgacggaa ggaatatgtt tcgcgctcgc
     1081 catgtcggtg gacggatttt tcggcggtct gggggcggcg tttctggata tttcgattgc
     1141 cggcacggta tgcctgaatc tggtattcag tgcacttgcg gtgcttggcg gaagcgctgt
     1201 cgggaaatat gcggcggacc ggtgcaaaag agattactcc tggatcggcg gcgccctgtt
     1261 tgtggtgctg gcattcagca agattttgta gatttatagt aaatgtgtca aaaaaagctt
     1321 gcgttttcat tgcggtatgg tataatatgc ctatacatga agcagtggaa acgcacagct
     1381 cttgtaaaat ataattagat attgtgggat ctgagagtgg agatgatttt aattcctgta
     1441 cacatgaggg gtgcttacag gttttatgac gatgtctcct ttttttatgc gctgcgaata
     1501 gggagcacgg ggcaggaacg gatttcacaa tagagggaag cataaataag caataagggg
     1561 caaacaagga ggaacatggt atggcaaagt atgtgatggc actggatgca ggaacgacga
     1621 gcaaccgttg tattttattt aacgagcagg gagagatctg ttcgatggca cagaaagagt
     1681 tccggcagtt ttttccgaat ccgggatggg tggagcacga tgccaatgag atctggtcta
     1741 caatgcttgg cgtggcggtc gaggcgatga acatgatccg cgcgacggcg gcggatattg
     1801 cggcgatcgg tatcaccaat cagcgtgaga cggcgattgt ctggaacaag gagactggag
     1861 aaccggtcta caatgcgatc gtgtggcagt gccgcagaac ttcggacatt gcggatgcat
     1921 tgaaggcgaa ggggctcgag gagacatacc gtcagaagac gggactgatc attgacgcgt
     1981 atttttctgc gacgaagatc aagtggattc tcgataatgt tgagggtgcg agagagcttg
     2041 cagagcaggg caagctgctg ttcggaacgg tggagacctg gctgatctgg aagttcacca
     2101 agggtgcggt gcatgtgacc gattattcca acgcatcccg tacgatgctg ttcaacatca
     2161 acgatctgtg ctgggacaag gagatcctgg aggagctgga gattccagag tgcatgctgc
     2221 cgacaccgcg tccgtccagc gaagtctacg gctacacgga tccttcgttt ttaggagatg
     2281 cgatcccgat tgcgggagct gcgggtgacc agcaggcggc gcttttcgga cagacctgtt
     2341 ttcacgaggg cgaggcgaag aatacctatg gaaccggatg tttccttctg atgaataccg
     2401 gagagaagcc ggtattttcc aagaatggtc tggtcactac gattgcgtgg ggactcgacg
     2461 gaaaggtgaa ttacgcattg gagggttcca tctttgtggc gggtgctgcg attcagtggc
     2521 tgcgcgacaa catgaagatg attgactctt ccatggattc agaatatatg gcgaagaagg
     2581 ttcacggaac acacggctgc tatgtggttc cggcgtttac gggactcggt gcgccgcact
     2641 gggatcagta tgcgagaggc acgatcgtgg gattgacgag aggaacgaac aagaaccaca
     2701 ttatccgcgc gacgctcgaa tcgattgcac tgcaggtgtg cgacgtcatc gatgcgatgc
     2761 gtgcagatgc cggcattgat ttaaagtccc tgaaagtcga cggcggtgca agtgcgaata
     2821 atttcctgat gcagtttcag gcggatatga tcaatgcgcc ggtgaagcgt ccgcagtgcg
     2881 tcgagacgac tgcaatggga gccgcttatc tggcaggtct ggcagttgga tactggaagg
     2941 ataaggagga cgtggtcaac aaccagaaga tcgaccgcat ctttaccccg gagatggagg
     3001 aggaagagcg caaggcaaaa cgcaagggct ggaataaggc agtcaagtat gcgtacggat
     3061 gggcaaaaga cgagccggag gaagaagagg aatagaaggc gcgttagaag caagcttacc
     3121 atatactata ggatgaaacg aaaccgcgca gagccggaaa cggactcgcg cggtttttca
     3181 ctccagagga tgcggtgctt cctgtgcagc tgccggtatc ccggatattg ttgaattttc
     3241 agattcatgg tagaatgagt taaaatgtga tggaagagtt ctatggatcg ggcattgtga
     3301 aagaggagca gatacagatg gagattcgat tattatacgg aaatgagata cagtgggcgg
     3361 tgcagacggc gcatgaggtc tatgagcagt gtgtgcgtcc gtatacaaga aatgcggcgg
     3421 aggtgcagca gtattatgac tatatgaatc tgcccaatct ttcacaggag atgctggcgg
     3481 gaaggctgtt tttatggggc gcatttgaaa acgggcagat gtgcggcgtc agtgccatgc
     3541 agaatgtcgg acatatcacg gcgctgtatg taagaccgca gtattttaga agacggatcg
     3601 gcagcaatct ggtggaccgg atgtgcaact ttgcggcatc ccgattgcag tgcgggcgag
     3661 tgacggtgca ggctgcgccg tcagcagcga attattttta ccacacggga tttacgctga
     3721 ttcagggggc accggcggga atgtacgtgc cgttggagag aagaatctgg tcggttccga
     3781 tgacccaggg atacatggga aatatcgggg gaagcggcag tacatccacg cagggcggtg
     3841 gtgtgccggt ggcgcagccg cggcgcacaa agccggagat cacttatccg aaaaagaagg
     3901 taagcgcccg ggtggtgctt tggcttactg cggtaaccat cgccttcgca gtggcggtag
     3961 tgacgggaat taccgtatat catatcacac aggagggaat cacgggcgga tatgagatgc
     4021 ctgatccggt gggggacgag aatacggaaa cggacgatgg acaggaactg tagcaaaaga
     4081 gcaggcattt aaccggacaa agaagcgcag gacgcgaagc ggaaggagac ggaaggtgga
     4141 tacgacgaca aagggaaccc gcacaaaata ccgtctggcg gaggcgatga aggaatgcat
     4201 gaagacgaca ccggtcgata acattaccgt gacacagctg acggaattgt gcggtgttac
     4261 aaggcagaca ttttatcgca attttattga caagtacgat ctgatcaact ggtattttga
     4321 tattttgctt cacaagtctt ttgagcatat ggggagcggg aaaaatattt atgag
//
LOCUS       DBMDQJ010000120         4373 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7563_length_4373_cov_1.606039, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000120 DBMDQJ010000000
VERSION     DBMDQJ010000120.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4373)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7563_length_4373_cov_1.606039"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            199..1029
                     /locus_tag="ACZO9R_03540"
     CDS             199..1029
                     /locus_tag="ACZO9R_03540"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0912179.1"
                     /translation="MINNHKKVVFLDVDGTMVNDRGEIPESTKEAVRRAKANGHKMVV
                     CTGRSRFQIYDELLELGFSGIVGAAGVFVIADGKEIYHAYIDEEHRKSVYDYLEGNGF
                     LFCYQADDGVVLNRRSSEGILEIYRKMGMSEERLVRLTGNMHLTEEPWKNPKNEKLLY
                     YNAPFPVAKVHADLEPYFDTVAISLEGMGEYAGEIGINGINKATGMERYLNAAGIARE
                     DSIAVGDGPNDLQMMEYAGIGIAMGNAREEVKERADMVTSSINNDGIYRAFETLGLLA
                     "
     gene            1041..1901
                     /locus_tag="ACZO9R_03545"
     CDS             1041..1901
                     /locus_tag="ACZO9R_03545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912180.1"
                     /translation="MEKRKLRILTVVVFAGVLIFGALRENSTGRGELGEIQVAMWNLG
                     KVDNLQLSYEYCWKQNGSAGGETMHAWADMLTGDWISEHYTTDEDGTRLYLKQFCDGR
                     NLYHYIDWSGEWEQILPQQRENTVIPDYEMVTDLGYDGTDVEDLERVTEDGAVEITCS
                     FTQEYLEEAWENQVAQVEKSYAFYEKSGADENAVKTAALSVQQHKQAHYEDMTATYVI
                     DENQILRSIEYQITLIMPEITQDLSGNKMLGKDRKMQIMVTAEVDRYNQGGIANKVQQ
                     YKTLVQEYME"
     gene            2090..2758
                     /locus_tag="ACZO9R_03550"
     CDS             2090..2758
                     /locus_tag="ACZO9R_03550"
                     /inference="COORDINATES: protein motif:HMM:NF024252.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0912181.1"
                     /translation="MDQTRIGTFIAVLRKEKGLTQKELAEQIGISDKTVSKWETGNGM
                     PDIAYLSPLCEVLDINVNELLSGEKLPSEEYLGKAEENMKHLMQENQNNKNAGRWQGL
                     AGIALLAVAVFLAFGLSQAKIGWYVDCPSLILMVCICVACVFFSGCRTKYEVVHFLRK
                     IVLPAGLLVMAIMVPVVIYMLSDPSAIGPGIAVMVLTLLYASAAYLVLYIIETRMERR
                     RKQN"
     assembly_gap    3124..3133
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3401..>4373)
                     /locus_tag="ACZO9R_03555"
     CDS             complement(3401..>4373)
                     /locus_tag="ACZO9R_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR0912182.1"
                     /translation="HLVCLRPWNEVLKADDNQISEDYSMLERRMEHFLGLHYTENTIE
                     NGMKRLSVIKTPFNKTNIDRINRQMLKISGQRDTLKERWNSALAVYDKMDIVDETEVK
                     DKFVTSVVFWDAILTMILSGILCLIGAIGAGIVAAVSQNGILAGTCYFFIAAGLAGIM
                     IRFPKIFMLGSPLKRLKAFGNGIRKALEEQQLLEETHCKVETESNGPDNHIIYLSGGS
                     GRDKALFAQCVNEFFDVIDNQRYILVKRKGHKGLNGFYAIPNCFAKKKEDAERFEKCM
                     HPYIGNYDCVYTRNEKGRELLLEGRVKALANREERCISRKKVKGALE"
BASE COUNT         1220 a          883 c         1134 g         1126 t
ORIGIN      
        1 gatcattgat aaatatacga tcgggtgcaa gaatagcagc cgatccggcg gctataggaa
       61 ggacaggtaa ggtgggaggc aaaaaataaa gacacttttt tccatataac gtttaaaata
      121 gcttgattcc atccccaaaa caacatatca tgtacataaa agcaatcgtg aagaggacag
      181 gaaaatatga gcgcagaaat gataaataac cataaaaaag tggtgtttct ggatgtggac
      241 ggcacgatgg tcaatgaccg gggagagata ccggaatcta ccaaagaggc ggtccgcagg
      301 gcgaaggcga acggtcacaa gatggtcgtc tgtacgggaa gaagccgttt ccagatttat
      361 gatgagctgc tggaacttgg gttttccggg attgtcggcg cggcaggcgt gtttgtgatc
      421 gccgatggga aggaaattta tcacgcatat attgatgaag aacacaggaa gagcgtttac
      481 gattatctgg agggcaacgg attcctgttt tgctatcagg cggacgacgg tgtggtgttg
      541 aaccgccgca gcagtgaagg aatcttagag atctaccgaa agatggggat gagcgaggag
      601 cgccttgtgc gtctcactgg aaacatgcat ctgacggaag agccttggaa gaatccgaaa
      661 aatgaaaagc ttctatatta taatgcgccg tttccggtgg cgaaggtaca tgcggatctg
      721 gagccttact ttgacacggt ggcgatcagt ctggaaggta tgggagaata tgcgggcgag
      781 atcggcatca acggaatcaa taaggcaacg ggcatggagc gctatctgaa tgcggccggc
      841 attgcgagag aggattccat tgccgtggga gacggtccga atgatcttca gatgatggaa
      901 tacgcaggaa tcggtattgc gatgggcaac gcgagagaag aagtcaagga acgggcggat
      961 atggttacat cgtccatcaa caatgatgga atttaccggg catttgaaac attgggattg
     1021 ctggcataga ggatagtgct atggaaaaaa gaaagctgcg gatactgaca gtcgttgtat
     1081 ttgccggtgt gctgatattt ggcgcactgc gcgagaacag tacgggaaga ggagaactcg
     1141 gagagattca ggttgccatg tggaatctgg gaaaagtgga caatctgcag ctttcctatg
     1201 aatattgctg gaagcagaac ggaagcgccg gaggagagac gatgcatgcc tgggcggata
     1261 tgctgacagg tgactggata agcgaacact acacgacgga tgaggacggg acaagactct
     1321 atttaaagca gttctgtgac gggaggaatc tgtatcatta cattgactgg agtggtgagt
     1381 gggagcagat actgccacag cagcgggaga acaccgtcat tcctgattat gaaatggtga
     1441 cagatctggg ttacgacggg acagatgtgg aagacctgga gcgtgtcaca gaggacgggg
     1501 ctgtggagat tacctgctct tttacgcagg aatatttaga ggaggcatgg gagaatcagg
     1561 tggcacaggt ggagaagtcc tatgcttttt atgaaaaaag cggggcagac gagaacgccg
     1621 tgaagacggc ggcgctatcc gtccagcagc acaagcaggc acattacgag gatatgacgg
     1681 cgacctatgt gatcgatgag aatcagattc tgcgaagcat tgaatatcag attacgctga
     1741 ttatgccgga gatcacacag gatctttccg gcaataagat gcttggaaaa gatcgcaaga
     1801 tgcagattat ggtgacggcg gaagttgacc gctacaatca gggcgggatt gccaataagg
     1861 tgcagcagta taagacactg gtgcaggaat atatggagta agagaaaaag ctgcgcagag
     1921 agacgatcta cgcggctttc tttgttttat agagcgtatt ctacggtgca gggattgtac
     1981 tttacgggtc ggtgaactaa ggtgtagcta taaagctgca gctttacaaa agcatgacag
     2041 gtccctcgga acggtggaca gcgacctgtc cggaaaatgg aggaagtata tggatcagac
     2101 gaggatcgga acatttatcg cagtattgag aaaggaaaaa ggtctgacac agaaagaact
     2161 ggcagaacag attggcatca gcgacaagac ggtatccaag tgggagaccg ggaacggaat
     2221 gccggatatt gcatatctgt cgccgctctg tgaggtgctt gacatcaatg taaacgaact
     2281 tctgtcgggt gaaaagcttc cttccgaaga atatcttgga aaggcggaag aaaatatgaa
     2341 acatttaatg caggagaatc agaataataa aaatgcaggc agatggcagg ggctggcggg
     2401 aatcgcattg ctcgcagtgg cagtctttct ggcatttggt ttgtcgcagg cgaaaatcgg
     2461 ctggtatgtg gactgcccgt ccctcattct tatggtatgt atctgcgtgg cgtgcgtgtt
     2521 tttcagcggc tgccgtacca aatatgaggt ggttcacttc ttaagaaaga ttgtgctgcc
     2581 cgccggtctg ctggtgatgg ccatcatggt tccggtagtg atttacatgc tgtcagaccc
     2641 gtcggccatc ggaccgggga ttgcggtgat ggtgctgaca cttctgtacg catcggcagc
     2701 gtatcttgta ctttacatta tagagacgcg catggagcgc aggaggaagc agaactagcg
     2761 cgccgtccct gcggctatgg gaagagagcc cctggtggct ccgtttcaca gacaatataa
     2821 aaccgcgcga accgaactcg ccggcatctg ttattgaaaa cagggcagat gcagactcag
     2881 acatcggctt cgcgcggtta tattttgtta gacaacaggt ggcgtacggt aggcgggagc
     2941 tgggcagttg ttgtaatagc cagtcaaata tggtatggta aaacaatcta acaaatcagt
     3001 atctgtctat gaatagcacg gttagacttg tacttgggat agcaatacat tgtctagtaa
     3061 tatatcaaac gtatgtatta tggattaaac gccaaaatgc aatagacata agttaaccat
     3121 ttannnnnnn nnnatatgtg tatctgtagt atttattatg tttttctttc aaccatttat
     3181 ttcatctttg ttatgggaaa tggttctgta aattccagat taacggatgt aatgtagcag
     3241 attttcacaa cttggatatt gcatgctgca catccttgaa tgaataaaaa atagaactga
     3301 tttgttttca gaattttagt accgctgcca gcgacaacaa gcggaagctc tcctgaaaac
     3361 aaacagttct attttttata tttcgttgtc aatatgtttt ctattctaac gcccctttta
     3421 cttttttgcg ggaaatacac cgctcctccc gattggcaag tgccttcacc ctgccttcta
     3481 acagaagttc tcttcctttt tcatttcttg tatacacaca gtcatagttt ccaatatacg
     3541 gatgcatgca tttttcaaaa cgttccgcat cctccttctt tttcgcaaaa caattcggaa
     3601 tggcatagaa accgttcagt cctttgtgtc cttttctttt cacaagtatg tagcgctggt
     3661 tgtcgatcac atcaaaaaat tcattgacac actgtgcgaa caacgcttta tccctgccgc
     3721 ttccaccgga cagataaata atatggttat ccggtccgtt tgattctgtc tcaactttac
     3781 agtgtgtttc ttccagaagc tgttgctcct ctaatgcttt gcggattcca tttccaaatg
     3841 cctttaatct ttttagcgga cttcccaaca tgaaaatttt cgggaaacgt atcataattc
     3901 cagccagtcc ggcagcaatg aagaagtaac aggttccggc caggatacca ttttgggaca
     3961 ctgcagcaac aatacctgcc ccaattgccc cgatcagaca cagaattcct gacaggatca
     4021 ttgtaagaat cgcatcccag aaaacaacac ttgtgacaaa tttgtctttt acctctgtct
     4081 catccacaat atccattttg tcatagactg caagtgcact gttccaacgt tcttttaacg
     4141 tatcgcgctg ccccgatatt ttcagcattt gtctgttaat cctgtcaata ttcgttttat
     4201 taaatggcgt tttaataact gacaaccgtt tcattccatt ttcaatcgta ttttctgtat
     4261 aatgcagacc gagaaaatgt tccatccttc gctccagcat actgtaatct tcactgatct
     4321 gattgtcatc tgcctttagc acttcgttcc acggtcttaa acagactaaa tgc
//
LOCUS       DBMDQJ010000121         4372 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7565_length_4372_cov_1.861157, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000121 DBMDQJ010000000
VERSION     DBMDQJ010000121.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7565_length_4372_cov_1.861157"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="ACZO9R_03560"
     CDS             complement(<1..1245)
                     /locus_tag="ACZO9R_03560"
                     /inference="COORDINATES: protein motif:HMM:NF012415.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR0912183.1"
                     /translation="MKKIRRSLAVFIAAFVMITGAGLLTEKTVPVRAEDNVTAFVDRM
                     YQVCLGRAADEEGRADWVNRLQTGEARGADVAYGFVFSTEFRNMNLCNSCYVDAMYQA
                     FFGRTADEAGKADWMNRLAEGQTRGAVMTGFVNSEEFSALCASYGIESGSGDWSDISI
                     PILGNCSWCGADNDTITDFVTRLYRICLEREPDEAGLADWSTQLANGAEGSQVAYGFI
                     FSTEYKQKHTSNTEFATMLYHTMMDREPDDAGLTDWVDKLNYTNTREYVFNGFLFSTE
                     FARRCAASGINIGNAVETPDATDAWQMNVQILALCNEQRQNNGLEKLMTREDLWEQVA
                     QVRAGEIVNYFSHTRPNGSSCFSLYDEAGLDYRAAGENIAAGYYGASAVVDGWMNSTG
                     HRANILSESYDYLATGYATGGAY"
     gene            complement(1299..4145)
                     /locus_tag="ACZO9R_03565"
     CDS             complement(1299..4145)
                     /locus_tag="ACZO9R_03565"
                     /inference="COORDINATES: protein motif:HMM:NF040368.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR0912184.1"
                     /translation="MEIMKRRVLAAFLAMILTCGVAFPGNAVYAAEVTETTVESDAAV
                     EETAVQTTDAEAEAKPEGSTEQPETTEAAEAAVTEATETEETEATEAAESKPVTEMSA
                     AEDVAAVKVTENMKYAASGKCGDNLTWSYNKGVLTISGSGAMYDYPVYDTPGSPQCMP
                     WYAYKTSITTVNIAEGVTSIGACTFYGCSSLTSVTTPSTISTIGYAAFADCTRLQQAT
                     IKSGFVGESAFIRCTALNTVSVGSGVTGFGISAFENCTGLTGVYISDLAAWCRIDFGG
                     YLANPLQFAKHLYLNDSEVTNLQIPDGVTAISDYAFEYGQNITSVTVPSSVTTIGDYA
                     FYLCNGIRTVNLPAGGLTRIGASAFDSCSGITSIAIPNSVSYIGTFAFAWAPIESANI
                     YQGVIESHAFEGCGCISNVTIGSGVTYIGDNAFNRCAGLRTVQYGGSRAQWRALEIGA
                     NNEALTGASVTCSGSGSASTDGVDRTKIHVGGTVKYGSYEQDNNASNGAETIEWTVLD
                     IQGDKALVISKKVLDFQRYYPNLQTTVTWANSSIRTWLNDSFYNAAFSDGQKSGIYTT
                     SVSGESNTVFGTSGGSATGDKIFLLSASEAANYLNTDGKRMANCTEYALSRNGDPALR
                     NTNTQSSYWWLRTPGIYTYDAMYVHYTGSLRYDGMAVANVIGGVRPAMWVNKNVVEVV
                     PESNRVITEDPIEQFVTRLYQVCLNREPDDAGLNDWVNRLSSGQASGVEVSYGFVFSQ
                     EFQNYNYCNTDYVKQLYRAFMGREYDQGGLDDWVGRLETGTTREEVFNGFSQSEEFNN
                     LCTQYGITRGDGIAVPQYGTVPRGACTVCGATDGVTAFVTRLYNICLDRNPDTDGLND
                     WTNGLWDHTKSGGSVAQGFIFSQEFKNKNLNDNDYVEYLYRAFFDRSADAGGKADWVS
                     RMQTQGYSREDVFNGFVGSEEFNNLCKKYGITRD"
     gene            complement(4234..4362)
                     /locus_tag="ACZO9R_03570"
     CDS             complement(4234..4362)
                     /locus_tag="ACZO9R_03570"
                     /inference="COORDINATES: protein motif:HMM:NF025314.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR0912185.1"
                     /translation="MYDDEGLNDWVNQLNNGTSRETVFWGFANSQEFANMVASYGL"
BASE COUNT          991 a         1344 c          898 g         1139 t
ORIGIN      
        1 gtaagctccc cctgtcgcat agccggtcgc aagatagtca tacgactcac ttaaaatatt
       61 ggctctgtgt cccgtagaat tcatccagcc gtccaccaca gcagatgctc cgtagtatcc
      121 tgccgcaata ttttctccag cagcacggta atccagcccc gcctcatcat agagtgaaaa
      181 gcagcttgac ccattcggac gtgtgtgcga aaaatagttc acaatctccc cggcacgtac
      241 ctgtgccacc tgctcccaca gatcctctct ggtcataagc ttttccagac cattattctg
      301 acgctgctcg ttgcaaagcg ccagaatctg cacattcatc tgccatgcat ctgtagcatc
      361 cggcgtctca accgcatttc caatattgat tcctgacgct gcacagcgtc ttgcaaactc
      421 tgtactgaac aaaaatccgt taaacacata ttccctggta ttggtatagt tcagcttgtc
      481 tacccagtct gtaaggcctg catcatccgg ttcgcggtcc atcatcgtgt gatacagcat
      541 cgttgcaaac tctgtgttag acgtatgctt ctgcttatat tctgtgctga atataaaacc
      601 atacgccacc tggctgccct cggcaccgtt agcaagctgt gtactccagt ctgcaagccc
      661 tgcctcatca ggctcgcgct ccagacagat gcggtacaat ctggtcacga agtctgtaat
      721 cgtatcattg tcagcaccgc accaggagca gttgccgaga atcggaatac tgatgtcgct
      781 ccagtcgccg cttccactct caataccata ggaagcacaa agcgcagaaa attcttccga
      841 attcacaaag ccggtcatga ccgctcctct ggtctgccct tctgccagac gattcatcca
      901 gtccgccttt ccggcttcat ctgccgtgcg tccgaaaaac gcctgataca tggcgtccac
      961 atagcagctg ttgcagaggt tcatattgcg gaattccgta ctgaacacaa atccgtacgc
     1021 cacatcagcg cctcttgcct ctccagtctg cagacggttc acccagtctg ccctgccttc
     1081 ctcatccgct gcacggccaa ggcacacctg gtacatccgg tccacaaatg cggtgacatt
     1141 atcctccgcc cgcaccggaa cggtcttctc cgtcagaaga ccggctcctg taatcatgac
     1201 aaaagcagca ataaaaaccg ccagcgatct tcgtatcttt ttcattcagt tcctccctat
     1261 ctctggttga cgcagattgc aaactctgtc atcccacttt agtctctcgt tattccatac
     1321 tttttgcaca gattattgaa ttcttcactg ccgacaaatc cgttaaatac atcttccctg
     1381 ctgtatccct gcgtctgcat gcggcttacc cagtctgcct tgccgcccgc atctgcactc
     1441 cggtcaaaaa atgcacggta cagatactcc acataatcat tgtcattcag attcttattc
     1501 ttaaactcct gggagaaaat aaatccctgc gctactgaac cgccgctctt cgtgtgatcc
     1561 cacagtccgt tcgtccagtc gtttaatccg tccgtatccg gattgcggtc caggcagata
     1621 ttataaagtc tggtcacaaa tgccgtcaca ccgtctgtgg caccgcagac cgtacatgct
     1681 cctctcggaa ctgttccgta ctgtggaacc gcaataccgt ctcccctcgt aataccgtac
     1741 tgggtacaca gattgttaaa ctcttcgctc tgcgaaaatc cgttgaatac ttcctccctg
     1801 gtcgttcctg tctcaagtct gccgacccag tcatccagac cgccctggtc atattcacgt
     1861 cccatgaacg cgcgatagag ctgctttaca tagtcggtgt tgcagtaatt gtaattctgg
     1921 aattcctggc taaacacgaa tccgtatgac acctccacac cggatgcctg tccggatgac
     1981 aatctgttca cccagtcatt taagcccgca tcatccggtt cacggtttaa gcagacctga
     2041 tacagccggg ttacaaactg ttcaatcgga tcttccgtaa tcacccggtt gctttccggc
     2101 acgacctcga ccacattctt atttacccac attgccggac ggacaccgcc gatcacattc
     2161 gccactgcca taccgtcgta gcggagcgaa cctgtgtaat gtacatacat ggcatcatat
     2221 gtatagatgc ccggagtccg cagccaccag taagagctct gtgtatttgt attgcgaagc
     2281 gccgggtcgc cgtttctgct aagagcatac tctgtgcagt tcgccattcg cttgccgtcc
     2341 gtattcagat agttggcagc ctccgatgca ctgagcaaaa agatcttatc accggtcgcc
     2401 gatccgccgg atgttccgaa tacggtattc gactcaccgg ataccgatgt tgtgtaaatc
     2461 ccggacttct gtccatcgct aaacgccgca ttatagaagg aatcattcag ccaggtacgg
     2521 atactgctgt tcgcccatgt aaccgttgtc tgcaggttcg gataataccg ctggaaatcc
     2581 aggaccttct tgctgatcac cagcgcctta tcaccctgaa tatccagtac cgtccactca
     2641 atggtctcag cgccgttgga agcattgtta tcctgctcgt aagaaccgta ttttacagtt
     2701 ccgccgacat gaatcttcgt ccggtccacg ccgtcggtgc tggcacttcc gcttccgctg
     2761 caggtaacac tcgcgccggt cagtgcctca ttatttgctc cgatctccaa cgctctccac
     2821 tgcgctctgg aaccgccgta ctgcactgtg cgcaatccgg cacaccggtt aaatgcgtta
     2881 tcgccgatat atgtaacacc gcttccaatc gtaacattcg agatacatcc gcatccctca
     2941 aatgcatggc tctcaatcac gccctgatag atattggcgg attcaatcgg cgcccacgca
     3001 aacgcgaacg ttccaatgta ggacacacta tttgggattg caatactggt aattcccgag
     3061 cagctgtcaa atgcacttgc cccgatgcgg gtcagtccgc cagccggcag attcaccgtc
     3121 cggatgccat tgcaaagata aaatgcgtaa tcaccgattg tagtcacact ggaaggaacc
     3181 gttacgcttg taatattctg tccgtattca aatgcgtaat cgctgatcgc agtcacaccg
     3241 tccggaatct gcagatttgt cacttccgaa tcattcagat acaaatgttt tgcaaactga
     3301 agcgggtttg ccagatagcc gccaaaatca atcctgcacc atgcagcgag atcgctgata
     3361 tacacgccgg tcagtccggt acagttctca aaagcactga ttccaaatcc ggtcacaccg
     3421 cttcccaccg aaacggtatt aagtgctgta cagcggataa acgccgactc ccccacaaag
     3481 ccggacttga tcgttgcctg ttgaaggcgg gtacaatctg caaatgccgc atacccgatc
     3541 gttgagatcg tcgacggtgt tgtcacactc gtcagtgaac tgcatccgta aaaagtacat
     3601 gccccgatgg atgtcactcc ctctgcaata tttaccgtcg taatcgatgt tttataggca
     3661 taccacggca tacactgcgg ggatcccggc gtatcatata ccggataatc atacatcgca
     3721 ccgcttccgc tgatcgtaag cacgccctta ttatagctcc atgtcagatt gtcgccgcac
     3781 ttgccggaag ctgcatactt catattctct gtcactttta cggcagccac atcttctgcc
     3841 gcgctcatct ctgtcacagg cttgctctcc gccgcctctg ttgcttccgt ctcttccgtc
     3901 tcggtcgctt ctgtcactgc tgcttcggct gcttctgttg tttccggctg ctctgtgctt
     3961 ccctccggct tcgcttctgc ctccgcatcc gttgtctgca ccgccgtctc ttcaacagcc
     4021 gcgtcactct ccaccgttgt ctccgtcact tccgctgcat agactgcatt gcccggaaat
     4081 gccacaccac atgtgagtat cattgccaga aaagccgcta acacacgtct cttcataatc
     4141 tccatacctc actttccccg tatactacag aaggaatcct gccggattcc ttcccttact
     4201 gctactatag cattttatat acgttttgtc catttataat ccataactgg caaccatatt
     4261 tgcaaattcc tgtgagtttg caaaacccca gaatacagtc tctctggagg tgccgttgtt
     4321 cagctggttc acccagtcat tcagcccctc atcgtcatac aagcgtccga aa
//
LOCUS       DBMDQJ010000122         4368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7579_length_4368_cov_1.457699, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000122 DBMDQJ010000000
VERSION     DBMDQJ010000122.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7579_length_4368_cov_1.457699"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..1036
                     /gene="prfB"
                     /locus_tag="ACZO9R_03575"
                     /pseudo
     CDS             20..1036
                     /gene="prfB"
                     /locus_tag="ACZO9R_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_155942212.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            1026..1712
                     /locus_tag="ACZO9R_03580"
     CDS             1026..1712
                     /locus_tag="ACZO9R_03580"
                     /inference="COORDINATES: protein motif:HMM:NF016190.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HIR0912186.1"
                     /translation="MSSDVIRTLKWVFGKGGLIEEYKRQEQMLEETPKSVASMTRIFA
                     PQISRDFPGMNLEQFEALAQQQLVAALQAVSAGNLESVARASDELKEQVSRQIEGNRA
                     AGVRETYEQIRIHQTEISNYEKKNGTCVITFQSAVEHYHYKEKAGKIIWGYKDRKLQT
                     KYNMELLYVQDAAQQTEGNAFGLNCPNCGAPMKGLGQQICEYCGSPVVPVDTRVWRMV
                     RFSEVDYHHV"
     gene            1814..3136
                     /locus_tag="ACZO9R_03585"
     CDS             1814..3136
                     /locus_tag="ACZO9R_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR0912187.1"
                     /translation="MGIIKAIGSAISGGLADQWLEVFEADDMGEQTVFTSGVLIRRGQ
                     NTKGTNNTVSNGSVIHVYDNQFMMLVDGGKVVDYTAEPGYYKVDNSSLPSMLNGELGE
                     SVKETFNRIKYGGQTPTAQKVYFLNLQEIKGIKFGTRNPINYFDNFYNAELFLRAHGN
                     YSIKITNPLQFYAEVIPRNKDRVNYDEIGEQYLSEFLEALQSAINQMSADGTRISYVT
                     SKAMELGKYMQDILDADWNQMRGMEIQKVGIAGISYDEESQKLINMRNEGAMLGSDFN
                     VMRGMAVKNITEGVRDAGSNANGAMAGFMGVGMGMNAMNQTLSGLGAMQTPADMQANA
                     GMQQGSAAPAGQMPAAGAVPAGQTPGAGTQVGWTCECGHVNTGKFCSECGKPMPVSDE
                     WTCECGHVNTGKFCSECGKPKPVSGEWTCECGQTNTGKFCSNCGKARN"
     gene            3139..4254
                     /locus_tag="ACZO9R_03590"
     CDS             3139..4254
                     /locus_tag="ACZO9R_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HIR0912188.1"
                     /translation="MAIISFKCPNCGGELIFDPATQKYKCEYCNSLFTQEELDAMKPM
                     QGGEPDAVAQEQAGGERADETEAAEQAAGERESEPEGEAVYYSCPSCGAEIVTDATTA
                     ATFCYYCHNPVVLGKRLEGTYLPNKIIPFEISREEAEKKFLDFVGKKKYVPKAFFDKK
                     QIESMTGVYFPYWVYDVELEGKMQGDARSVRMWTTGNTQYTETKYYAVEREGNVSLRN
                     LTENALKKANVKLTSGVMPYAYDKMKDFHMGYLSGFLAERRDIEQNAVQGKMQHTMRE
                     SAEKLMRDTISGYNSVSVKNTFFAPKKEIWSYVLLPVWTITYKGRDGKVYYYSMNGQN
                     GNICGDLPVDRPRLALTSLLIALGILVFGLLGGYFLW"
BASE COUNT         1169 a          921 c         1380 g          898 t
ORIGIN      
        1 ttttgaatcc gtggaatagg aagctgcgga tttctggaat gatccggagg tttcccagaa
       61 aaaaatgaaa gagttaaaga gcatgaagga cgatgttgcc acatatgctt ctttgacgac
      121 acagtttgaa gatattgaga cactgatcga gatgggatat gaggagaatg acgccacgtt
      181 gatcccggag atcgaagaaa tgctaatgga gtttacgacg acgtacgatg ccatccgcgc
      241 aaagacgctt ctgtccggcg agtacgatgg ggacaatgcc attgtgtcgc ttcatgcggg
      301 cgcgggcggt acggagtcct gtgactgggc agcaatgctt taccgcatgt atacacgttg
      361 ggcagatgac aagggataca gcgtggaagt gctggactcc ctggacggag aggaagcagg
      421 catcaagtcc gtgacatttc aggtaaatgg agaaaatgcc tacggctatc tgaaatcaga
      481 aaaaggtgtg caccgtctgg tgcgtatctc gccgttcaat gcggcgggaa agagacagac
      541 ctcttttgtg tcctgcgatg tcatgccgga tatcgaggaa gatctggatg tggagatcaa
      601 tgacgatgat cttcggatcg atacgtaccg ctccagcggt gcgggcggac agcatatcaa
      661 taagacatct tccgcgatcc gtatcacgca tcttccgacc ggtattgtgg tacagtgcca
      721 gaatgagcgt tcccagttcc agaacaagga caaagcgatg cagatgttaa aggcaaagct
      781 gtacctgtta aagcagcagg agaatgccga gaaggcggcg ggaatccgcg gcgaagtgac
      841 ggagatcggg tggggcaacc agatccgctc ctatgtcatg cagccgtata cgatggtgaa
      901 ggatcaccgc accggtgtgg agaccggtaa tgtggacagc gtgctggacg gaaagctgga
      961 tatctttatg aatggatatc tgaagtggct ctccctcggc tgcccgaagg gacttggagg
     1021 agacgatgag cagtgatgtg atccgcacct taaagtgggt gtttggaaaa ggcggtctga
     1081 ttgaggaata taagcgtcag gaacagatgt tggaggagac accgaagtcg gtcgcttcca
     1141 tgacgcggat ttttgcaccc cagatttccc gtgattttcc gggaatgaat ctggagcagt
     1201 ttgaggcgct cgcacagcag cagcttgtgg cggcgcttca ggcagtgtcg gcggggaatc
     1261 tggaaagcgt cgcgcgcgct tccgatgaac tgaaggagca ggtcagccgt cagattgagg
     1321 gaaaccgtgc ggcaggagtg cgtgagacct atgagcagat ccgcattcat cagacagaga
     1381 tttccaatta cgaaaaaaag aacggcacct gtgtgattac atttcagagt gcggtagagc
     1441 attaccacta caaggaaaaa gcgggcaaaa ttatctgggg atacaaagac cggaagctgc
     1501 agacaaagta caatatggag ctgctctatg tacaggatgc ggcgcagcag acggagggaa
     1561 atgccttcgg gctgaactgc ccgaactgcg gggcgccgat gaagggactt ggacagcaga
     1621 tctgtgaata ctgcggcagc ccggttgtac cggtagacac cagagtctgg cggatggtac
     1681 gtttttctga ggtggattat caccatgtgt gaatacattt ttggcgcatg attctgtgca
     1741 tatgaagttt tgcaggaact tccaataaga actcgtcaga gtacaacgaa agaatagaac
     1801 aggaggaatt gttatgggaa ttatcaaggc aatcggtagt gcgatcagcg gaggactggc
     1861 ggatcagtgg ctcgaggtgt tcgaggcgga tgatatgggg gaacagaccg tctttacgag
     1921 tggtgtgctg atccggagag gacagaatac caaggggacg aacaatacgg tgagcaatgg
     1981 ctccgtgatt cacgtgtatg acaaccagtt tatgatgctt gtggatggag gaaaagtagt
     2041 cgattacacg gcagaaccgg gatattataa ggtagataat tcttccctgc cgtccatgct
     2101 aaacggtgaa ctgggagaat ctgtaaaaga gacgttcaac cgtatcaagt acggcggaca
     2161 gacgccgacg gcacagaaag tatatttcct gaatctgcag gagatcaaag ggatcaagtt
     2221 cggaacgaga aacccgatca attactttga taatttttat aacgcagagc ttttcctgcg
     2281 cgcacacggt aattattcca tcaagatcac gaatccgctg cagttctatg cggaagtgat
     2341 cccgcggaac aaagaccgtg tcaattacga tgagatcggc gagcagtacc tgtcagagtt
     2401 tttagaggca ctgcagtcgg cgatcaacca gatgtcggcg gacggaacac gtatttccta
     2461 tgtgacttcc aaggcgatgg aactgggcaa gtacatgcag gacattctgg atgctgattg
     2521 gaaccagatg cgcggtatgg agatccagaa ggtcggtatc gcgggcattt cctatgacga
     2581 agagtcccag aagctgatca acatgcgcaa tgagggcgca atgctcggaa gcgatttcaa
     2641 cgtcatgcgc ggtatggcgg tcaagaatat tacggagggt gtgcgtgacg ccggaagcaa
     2701 tgcgaacggt gccatggcag gctttatggg agttggcatg ggaatgaatg ccatgaacca
     2761 gacgctgtct ggactcggcg cgatgcagac accggcagac atgcaggcaa atgcaggaat
     2821 gcagcagggc agcgcggcac cggcaggaca gatgccagcg gcaggcgcag tgccggcagg
     2881 acagacaccg ggggcaggta cccaggtggg ctggacatgt gagtgcggtc atgtgaacac
     2941 cggaaagttc tgcagcgagt gcggaaaacc gatgccggta tctgacgaat ggacgtgtga
     3001 gtgcggccat gtgaacaccg gaaagttctg cagcgagtgt ggaaaaccaa agccagtatc
     3061 cggtgagtgg acgtgcgagt gcggacagac gaacaccgga aaattctgca gcaactgtgg
     3121 gaaagcgagg aactagcgat ggcaattatc agttttaagt gtccaaactg tggcggagaa
     3181 ctgatttttg acccggcgac acagaagtat aagtgtgaat actgcaattc gctgttcacg
     3241 caggaagaac tggatgcgat gaagccgatg cagggcgggg agccggacgc tgtggcacag
     3301 gagcaggcag gcggagaacg ggcagatgag acggaagcgg cggagcaggc agccggagag
     3361 cgggaaagcg aaccggaggg cgaggcggtg tattacagct gtccgtcctg cggcgcagag
     3421 attgtgacgg atgcgacgac tgcggcaacc ttctgctatt actgccataa tccggttgtg
     3481 ctcggaaagc ggctggaagg tacctatctg ccgaacaaga tcattccgtt cgagatcagc
     3541 agagaggaag ccgagaagaa gttcctggat tttgtcggca agaagaaata tgtgccgaaa
     3601 gcattttttg acaaaaagca gattgagagc atgacaggcg tctatttccc atactgggtc
     3661 tacgacgtgg agctggaggg aaagatgcag ggagacgcga gaagtgtgcg catgtggacg
     3721 acgggcaata cccagtacac ggagaccaaa tattatgcgg tggagcgcga gggcaatgtg
     3781 tcgcttcgca atctgacgga gaacgcgttg aagaaggcga atgtaaagct taccagcggt
     3841 gtgatgccgt acgcctatga caagatgaaa gattttcaca tgggatacct ctccggattc
     3901 cttgcggagc gcagagatat tgagcagaat gctgtgcagg gcaagatgca gcacaccatg
     3961 cgtgagagcg ctgaaaagct gatgcgcgat accatcagcg gttacaacag cgtatcggtc
     4021 aagaatacgt tttttgcacc gaagaaggaa atctggtcgt atgtgctgct cccggtatgg
     4081 acgattacat ataagggcag agacggaaaa gtatactact attccatgaa cggacagaat
     4141 ggaaatatct gcggtgatct gccggttgac cgcccgagac ttgcgctcac cagtctgttg
     4201 atagcgcttg gcattctggt attcggactg ttaggagggt atttcttatg gtagagaaga
     4261 tgaaaaaatc gctcctgtgg tgtctggtat gcctggtgtg tcttgttccg tgcagcggtg
     4321 cggcactggc ggcggaggcg gacgcggcgc aggatgcagc ggatggtt
//
LOCUS       DBMDQJ010000123         4349 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7617_length_4349_cov_1.292844, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000123 DBMDQJ010000000
VERSION     DBMDQJ010000123.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7617_length_4349_cov_1.292844"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(43..1833)
                     /locus_tag="ACZO9R_03595"
     CDS             complement(43..1833)
                     /locus_tag="ACZO9R_03595"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912189.1"
                     /translation="MKKLLQYLGNYKKESILGPLFKLLEASFELIVPLVMAAIIDTGV
                     ANADRPYIMKMSLVLFLLALIGLTCAITAQYFAAKAAVGCATGLRHALFEHIETLSFT
                     EMDTVGTSTLITRMTSDVNQVQNGVNLVLRLFLRSPFIVFGAMVMAFTIDVKAALVFV
                     VTIPLLSVVVFGIMLVSIPLYKKVQAALDKVLGITRENLTGSRVIRAFNKEQDEIAQF
                     HEDNEALTKIQLYVGKISALMNPLTYIIINGAIVVLVWVGAIRVDNGNITQGQVVALV
                     SYMSQILVELIKLANLIININKSIACMNRIETVLDMESSITEHPAAETGSLTLDGAGR
                     EGKETIPAENAVVFSRVGLTYAGASEEALSDIDFTVRRGETIGIIGGTGSGKSSVVNL
                     IPRFYDATRGEVLVDGKNVRDYTLEELRAKVGVVLQKAVLFKGTIRENLSWGNSDATD
                     EEMYEALETAQAREFVDAKEGGLSYEIEQGGRNLSGGQRQRLTIARALVKKPQILIMD
                     DSASALDFATDAALRKAIAHMDGQMTVFIVSQRAASIMQADKIVVLDDGEIVGLGTHE
                     DLLKDCEVYREIYESQFKRTEEQQAGEAKR"
     gene            complement(1850..2722)
                     /locus_tag="ACZO9R_03600"
     CDS             complement(1850..2722)
                     /locus_tag="ACZO9R_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR0912190.1"
                     /translation="MYLVQFIRGFCMALADSVPGVSGGTVAFLLGFYDQFIDSIDDLI
                     TGSREKKKAALFFLIKLGTGWVCGMILAILILASVFDAHIYAISSLFIGFIIFAIPII
                     FREEKNCLAAKKSAIPFVLIGVAIVTAITYFNPSSGGEGVSLEHLTPGLALFVFLAGM
                     IAICAMVLPGISGSTLLLIMGLYLPVVTAIKDILHLNFAAFPTVFIFGCGVLVGIFGV
                     VKLIRKALDHFRAQTIYLIIGLMLGSIYAVVMGPTTLEVPQPAMTLHDFNILFFVIGG
                     ALIIGLQKMKDLKA"
     gene            complement(2786..>3834)
                     /locus_tag="ACZO9R_03605"
     CDS             complement(2786..>3834)
                     /locus_tag="ACZO9R_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912191.1"
                     /translation="LDQWCATLAIILMMVVVYLTVSVAYQFNRDTIGSLTEKEAAQRQ
                     MVDDIIAVADQVRKGTENAMNIVNELNSSTEVVNGAMKDISDSTQSTAENIQTQTEMT
                     QNIQDSIGTTLERSEKMVQVAKQSGELNEKSSRIMDDLKKQSAVIAQTNSEVASSMRE
                     LQDRTSAVKSIADTIFSISSQTNLLALNASIESARAGEAGRGFAVVADEIRQLAEKTR
                     QETENIANILEQLSENAQTAADAVERSVTAAGAQDEMIAQASESFGAMNQNVNELVTD
                     IGEIDTMLNNLSKANNQIVENIMQLSATTEEVTASSVQAADLSVKNLGNAENTKDLLH
                     SVLDESHKLEQYIH"
     assembly_gap    3835..3844
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3845..4270)
                     /locus_tag="ACZO9R_03610"
     CDS             complement(<3845..4270)
                     /locus_tag="ACZO9R_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912192.1"
                     /translation="MEQNKRANHFLAIGYIVFYLCVLCVVGIACLRGIRTVGYTVMIA
                     VLIILATAGTLVLYQRRKDDPKIRYIATVGLLVITFVMGWAFDNYYVRFMAAIPLVAC
                     ILFYDKKFAAFSGIAVSAVNLLVTASKTLALHTYQGENAL"
BASE COUNT         1075 a         1248 c          902 g         1114 t
ORIGIN      
        1 ccttgcgcag cgttgatttt tgattttcgt ttaccacttt tttcatcttt tcgcctctcc
       61 cgcctgctgt tcttctgtcc gcttaaactg cgactcgtaa atctcgcggt atacctcgca
      121 gtccttaagc agatcctcgt gtgttccaag tcctacaatc tctccatcgt ccagcactac
      181 gatcttgtcc gcctgcatga tggacgccgc acgctgcgat acgataaata ccgtcatctg
      241 cccgtccata tgtgcgatgg ctttccgaag cgccgcgtcc gtcgcgaaat caagcgcgga
      301 tgcgctgtcg tccataatga gaatctgcgg cttcttcacg agcgcacgcg caatcgtaag
      361 gcgctgtctc tgtccgccag aaaggtttct gccgccctgc tcgatctcat aggaaagtcc
      421 gccttccttg gcatccacaa actcccttgc ctgtgcagtc tcaagcgcct catacatctc
      481 ctcgtccgtg gcgtccgaat tgccccagct taagttctca cggatggttc ccttaaagag
      541 aaccgccttc tgcagaacaa caccgacctt cgccctgagt tcctccaatg tataatcccg
      601 cacattcttc ccgtcgacga gaacctctcc tctggtcgca tcgtaaaacc gcggaatcag
      661 attcacgacc gaagattttc cagaaccggt tcctccgata ataccgatgg tctctccgcg
      721 tctgaccgta aaatcaatgt cggagagcgc ttcctccgat gcgcccgcat aagtaagacc
      781 aaccctggaa aatacgacgg cattctccgc cgggattgtc tcttttcctt ccctgcctgc
      841 tccatccaaa gtgagacttc ccgtctccgc tgctggatgc tccgtgatcg acgattccat
      901 gtcaagcaca gtctcgatac ggttcatgca ggcaatggat ttgttgatgt tgataatcag
      961 gttggccagc ttgattaact ccactaagat ctgggacatg tagctcacca gcgccacaac
     1021 ctgtccctgc gtaatgttgc cattgtccac gcggatcgct cctacccata ccagaacgac
     1081 gatcgctccg ttgatgataa tataggtaag cggattcatg agcgccgaga tcttgccgac
     1141 atagagctgg atctttgtca gcgcctcatt gtcttcgtgg aactgtgcaa tctcatcctg
     1201 ctccttgttg aatgcacgga tgactctgct tccggtcagg ttctctcttg tgatgcccag
     1261 cactttatcg agtgccgcct gtactttttt gtacagcgga atactgacca gcatgatgcc
     1321 aaacacaacc accgacagca gcggaatggt cacaacgaac acgagcgccg ctttcacatc
     1381 aatcgtgaat gccatgacca tcgctccaaa cacgataaac ggcgaacgca agaacaggcg
     1441 cagaacgagg ttcaccccgt tctgcacctg attaacatca cttgtcattc ttgtaattaa
     1501 cgtcgacgtt ccgacggtat ccatctcggt aaatgacagc gtctcaatat gttcaaacag
     1561 agcatgtctt aatcctgtcg cacagccaac cgccgctttc gccgcaaaat actgcgccgt
     1621 gatggcacag gtcaatccga tcagcgccag gagaaacagt accagactca tcttcataat
     1681 atatggacga tcggcatttg ccaccccggt atcgatgatt gccgccatga caagcgggac
     1741 aatcaattca aatgaagctt ccaggagctt aaaaagcggt cctaaaatac tttctttttt
     1801 atagtttccc aaatactgca ataacttttt catgatactc cttcgtgtcc tatgctttta
     1861 aatctttcat tttctgaagt ccgataatca gcgcaccgcc gatcacgaaa aacaggatgt
     1921 taaaatcatg aagcgtcatt gccggctgcg gtacctccaa cgtggtcggt cccatgacaa
     1981 ccgcgtagat tgatcctaac atcagaccga tgatcagata gatcgtctgt gcccggaaat
     2041 gatctaacgc cttgcggatc agctttacca caccaaaaat accgacgaga acgccgcatc
     2101 cgaaaataaa caccgtcgga aatgcagcaa aattcagatg aagaatatct ttgatggcag
     2161 tcaccaccgg cagatacagt cccatgatta aaagaagcgt cgagcccgaa attcccggca
     2221 gcaccatcgc acagatggca atcattccgg caaggaatac aaacagggca agccccggcg
     2281 tcagatgctc caggctgaca ccctctccgc cgctgctcgg attaaagtaa gtaatcgcgg
     2341 tcacgattgc aacgccaatc agcacaaacg gaatggcaga tttttttgcc gcaaggcagt
     2401 tcttttcttc cctaaagata atcggaatgg caaaaataat aaatccgata aacagggaac
     2461 tgatcgcata gatatgagcg tcaaacacgc tcgcaagaat cagaatggcg agaatcattc
     2521 cacagaccca gccggttcca agcttaatca ggaagaacag agctgctttc tttttctccc
     2581 tgcttccggt tatcagatcg tcgatagaat caataaactg gtcataaaaa cccaacagga
     2641 aagcgacggt tccgccggat acgccaggta cactgtctgc cagtgccatg caaaatcctc
     2701 tgataaactg cactaaatac atcctttata cctcattttt ctgttatttt tctattttat
     2761 tcactacagg tcaaaatctg tccaattagt ggatatactg ctccagcttg tgcgactcgt
     2821 ctaacacact gtgaagcaga tccttggtat tttccgcatt gccgagattc ttaacactga
     2881 gatccgccgc ctgcacagaa gaagctgtaa cttcctccgt cgttgcggag agctgcatga
     2941 tgttctccac gatctgattg ttcgccttgg agaggttgtt taacatagtg tcgatctcac
     3001 cgatgtccgt cacaagctca ttgacattct ggttcatggc gccaaagctc tcggatgcct
     3061 gcgcaatcat ctcatcctgc gcacccgccg ccgtcacgga acgctccacc gcatccgccg
     3121 ccgtctgggc attttcagaa agctgctcta aaatgttggc aatattctcc gtctcctgtc
     3181 tggtcttctc cgccagctgg cggatctcgt ctgcaaccac cgcaaagcct cttcccgcct
     3241 cgcctgctct cgcactctcg atcgatgcat tgagtgccag aaggttggtc tgactggaaa
     3301 tagaaaaaat ggtatctgca atacttttga cagcgcttgt acgatcctgg agttctctca
     3361 tggaggaagc cacctcggag tttgtctgtg caataactgc ggactgcttc ttcagatcat
     3421 ccataattct ggaactcttc tcattcagct ctccggactg cttcgccacc tgtaccatct
     3481 tctctgaacg ctccagcgtc gttccgatgg agtcctgaat attctgtgtc atctccgtct
     3541 gcgtctggat attctcggcg gtactctgtg tactgtccga aatatccttc atggcaccgt
     3601 tgactacctc ggtggaacta ttcagttcat tgacaatatt catcgcattc tctgttccct
     3661 tgcgcacctg atctgcaacc gcaatgatat cgtccaccat ctggcgctgt gctgcttcct
     3721 tttctgtcag gctgccaatc gtatcgcggt tgaactggta cgccacggat actgtcagat
     3781 aaaccactac catcataagt ataattgcca gcgtcgcaca ccactgatcc agtgnnnnnn
     3841 nnnncagtgc attctcgccc tggtatgtgt gaagcgccag cgtctttgac gccgtcacaa
     3901 gaagattcac cgccgataca gcaatcccgg aaaaggcggc gaacttttta tcgtaaaaca
     3961 gaatacatgc aaccagcgga attgcagcca taaaacgcac ataataattg tcaaacgccc
     4021 atcccatcac aaacgtaatg acaagcagcc cgaccgtcgc aatatagcgg atcttcggat
     4081 catcttttct ccgctggtac aacacgagtg ttcccgctgt tgccagaata atcaataccg
     4141 cgatcataac ggtatatcca actgtgcgga ttcctctgag gcaggcaatt cctaccacac
     4201 aaagcacaca gagataaaac acgatatatc cgatagcaag gaaatgattg gcacgtttat
     4261 tctgttccac agtatctgca tacaaatgct tctgttcctt tttctccata actttcacct
     4321 ttcttctttc gataactcca tataccatt
//
LOCUS       DBMDQJ010000124         4337 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7636_length_4337_cov_1.406752, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000124 DBMDQJ010000000
VERSION     DBMDQJ010000124.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7636_length_4337_cov_1.406752"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            45..2207
                     /gene="gnpA"
                     /locus_tag="ACZO9R_03615"
     CDS             45..2207
                     /gene="gnpA"
                     /locus_tag="ACZO9R_03615"
                     /EC_number="2.4.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050500 -
                     1,3-beta-galactosyl-N-acetylhexosamine phosphorylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,3-beta-galactosyl-N-acetylhexosamine
                     phosphorylase"
                     /protein_id="HIR0912193.1"
                     /translation="MEQKKGRVTIPTNLDVVPETIELMKRWGADAIRDCDGTEFPEEL
                     VRTGAKIYATYYTTRKDNEWAKANPDEVQQCYVMTSFYTAVDTDLEIPLMKGISQELM
                     MVNTRDDKKRWWEVVDRSTGNVVSADHWEYEEEKGCVVIHDAIPFHEYTVSFLAYIIW
                     DPVHMYNAVTNDWKNFEHQITFDVRQPKTHKYSLERLRKYCADHPYVNVIRYTTFFHQ
                     FTLMFDELKREKYVDWYGYSASVSPYILEQFEKEAGYRFRPEYIIDQGYYNNQYRVPS
                     REFKDFQAFQRREVAKIAKEMVDITHEYGKEAMMFLGDHWIGTEPFMEEFATIGLDAV
                     VGSVGNGSTLRLISDIEGVKYTEGRFLPYFFPDTFCDGGDPVKEAKENWITARRAILR
                     KPIDRIGYGGYLKLTLDFPEFLDYVENVCNEFRELYENAKGTIPYCVRKVAVLNSWGK
                     IRSWGCHMVHHALYQKQNYSYAGIIEALSGAPFDVRFISFDDILANPEILKALDVIIN
                     VGDGDTAHTGGGIWENPAISAAIREFVYNGGGFIGVGEPSGHQFQGHYLQLADMLGVE
                     KETGFTLNYDKYNWEEHPDHFILADTTKPVDFGEGKKNIFAYEDTEILVQREKEVQMA
                     VHEFGKGRCVYISGLPYSFENSRILYRSILWSTHGEKDLHQWFSSNFNVEVHAYVKNG
                     RFCVVNNTYAHQKTVVYRGDGSSFPLEMEANEIKWYNI"
     gene            complement(2231..3829)
                     /locus_tag="ACZO9R_03620"
     CDS             complement(2231..3829)
                     /locus_tag="ACZO9R_03620"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0912194.1"
                     /translation="MVQLLIVDDEPLVQIGIRSMLNWESMGIEIAGTATNGRQAYDII
                     LEKHPEIVITDIKMPIMDGMQLIEKCQSQEHPPLFILLTSYEEFALVKQAISYQVLDY
                     LVKLELTEDVLNAAIQKALGILEKERKQAAIPETGSDLSLLQDNFYIRLLLNLFETEE
                     QFATQCDLLSVRFTAAGYTVAYLEMQNTRQSASAVASSDITLYRSSYQMIRNLIVKYV
                     PCRILFLDTTHLAIIFSLTKEQTGSYHQLLTDALTQTGDMLYNYYSIRILASVGSLVE
                     NPRDLSASYSDARQIFPDVRQSDSAVIFADEDNPAREEHNVFNIAIFKDELQKAFNEY
                     DPEALSAVFHDIIDLFSNDKRHYSQALSAASNVLHLTLSCLSNGEEQLNQIFGESANG
                     YNCLYEAETVPQVLSWMTVLCDGLCRLFSEHNKDYKNRTISAVKKYINEHVDEKITLN
                     QLSDVFNISPNYLSILFSKYNDMGFIDYVNHAKIECAKEMLDEGTLKIYEISDKLGYE
                     SAFYFSRVFKKVEGVSPRDYINRT"
     gene            complement(3873..>4337)
                     /locus_tag="ACZO9R_03625"
     CDS             complement(3873..>4337)
                     /locus_tag="ACZO9R_03625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912195.1"
                     /translation="SAELSLLDDYFTIQKYRYGGTISLEYRIDDEAALSCLIPRFTLQ
                     PVVENSIFHGIEPKGTPGTITIHIFRKNDAILQIDITDDGVGMTEEQARQLLSENKNE
                     KTEFFREIGVSNVHKRLQYEFGEDYGLRVESRLSEFTTVSVLLPFAPQEDIG"
BASE COUNT         1122 a          945 c         1133 g         1137 t
ORIGIN      
        1 ttaacaaccc ttgtaaataa aaaaaagaaa agcgaggtcg caaaatggaa cagaaaaaag
       61 gcagagttac tattcctacc aacctggatg tggtgccgga aaccatagaa ttgatgaaac
      121 gctggggtgc tgatgccatt cgtgactgcg acggtacaga gtttccagag gaactggtca
      181 gaaccggggc aaaaatttat gccacctatt atactacccg gaaggacaac gagtgggcaa
      241 aggcaaaccc ggatgaagta cagcagtgct atgtgatgac atccttttac actgctgtgg
      301 atactgactt agagattccc cttatgaaag gtatatccca ggaactgatg atggtcaata
      361 ccagagacga caaaaaacgg tggtgggagg ttgtcgaccg ttctaccggt aatgttgtgt
      421 ccgcagatca ttgggaatat gaggaggaaa agggctgcgt ggtcattcat gatgccattc
      481 ctttccatga atacacggtc agctttctgg cttacattat ctgggatccg gtacatatgt
      541 acaatgcggt taccaacgac tggaaaaact ttgaacatca gattactttc gacgtgagac
      601 agcctaagac ccacaaatat tccctggagc gtcttcgcaa atactgcgcg gatcatccct
      661 acgtgaatgt gattcgctat acaacctttt tccatcagtt taccttgatg tttgatgagt
      721 taaagagaga aaaatatgtg gactggtatg gctattctgc ctctgtcagc ccttatatct
      781 tagagcagtt tgaaaaggag gccggttaca ggttccggcc tgagtacatc attgaccagg
      841 gttattacaa caaccagtac cgtgtgccta gcagggagtt caaagatttc caggcattcc
      901 agcgcaggga ggttgccaaa attgccaaag agatggtaga cattacccat gagtatggca
      961 aagaggcaat gatgtttctt ggggatcact ggattggaac cgagccgttt atggaggaat
     1021 ttgccaccat tggacttgat gcggtggtag gcagtgtagg aaacggtagt acccttcgtc
     1081 tcatctccga tattgaaggt gtaaaataca cggagggaag atttttaccg tactttttcc
     1141 ccgatacttt ttgtgacggc ggagacccgg tgaaggaggc aaaggagaac tggattaccg
     1201 caagacgtgc cattctgaga aaacctattg accggatcgg ctatggtgga tatctgaagc
     1261 tgactctgga ttttccggaa tttctggatt atgtagagaa tgtgtgcaac gagttccgcg
     1321 agctgtatga gaacgcaaag ggaacgattc cttattgcgt gaggaaagta gctgtgttga
     1381 acagctgggg taaaattaga agctggggat gccatatggt acaccatgcc ctataccaga
     1441 aacagaatta cagctatgcg ggaattattg aagcattaag tggtgcccct ttcgatgtgc
     1501 gatttatttc ctttgatgat attctggcaa atccggaaat tcttaaagct ctggatgtga
     1561 ttatcaatgt gggagatggg gatactgctc ataccggtgg cggcatctgg gagaatcctg
     1621 ccatttccgc agctatccgt gagtttgtat ataacggcgg aggttttatc ggtgtcggtg
     1681 agccgtctgg tcatcagttt caggggcatt acctgcagct tgcagatatg ctcggcgtgg
     1741 aaaaagaaac cggatttact ttaaactacg ataaatataa ctgggaggag catcccgatc
     1801 acttcattct ggcagatacc acgaagccag tggactttgg tgagggtaaa aagaacatct
     1861 tcgcttacga ggacacggaa attctggtac agcgggagaa ggaagtgcag atggctgtcc
     1921 atgagtttgg aaaggggcgc tgtgtttata tcagcggtct gccttacagc tttgagaaca
     1981 gccgtattct gtaccgcagt attctgtgga gtacccacgg agagaaggat ctgcatcagt
     2041 ggttcagttc caacttcaat gtggaagtac atgcctatgt gaaaaatggc aggttctgcg
     2101 tggtcaacaa tacatatgcc caccagaaaa ctgtggttta tcgtggtgat ggcagcagtt
     2161 ttcctctgga gatggaggca aacgagatta aatggtataa catttaacac gggcaaaaga
     2221 aagaagtgcc tcaagtccgg ttgatatagt cgcgcggcga cacgccctcc acctttttga
     2281 atacccggct gaaatagaac gcgctctcgt accccagttt gtcggagatc tcatagattt
     2341 tgagcgttcc ctcatccagc atttccttgg cacattcaat ctttgcgtgg tttacataat
     2401 ctataaatcc catatcgttg tacttggaaa acaggatcga cagataattc gggctgatgt
     2461 tgaagacgtc ggagagctga ttcagcgtga tcttctcatc gacgtgttca ttgatatatt
     2521 tcttgaccgc ggagatggtt cggttcttgt aatccttgtt gtgctcggaa aacaggcggc
     2581 acagaccgtc gcacagcacg gtcatccagg agagcacctg cggcaccgtt tccgcctcgt
     2641 agaggcagtt gtacccgttg gcggattcac caaagatctg gtttaactgt tcttccccgt
     2701 tgctcaggca ggagagggtt aagtgcagca cgttgctcgc ggcggataag gcctgggagt
     2761 aatgtctctt atcattggaa aacaggtcaa tgatgtcgtg gaagactgcg gagagtgctt
     2821 ccggatcgta ttcgttgaac gctttctgca gctcatcctt aaagatggca atgttgaaga
     2881 cgttgtgctc ctcgcgggcg ggattgtcct cgtcggcaaa gataacggca gaatcactct
     2941 ggcggacgtc cgggaaaatc tgtctggcgt ccgagtagga ggcggacaga tctctcggat
     3001 tttcgacgag agagccgacc gatgcaagaa tccttatgct gtaataattg tagagcatgt
     3061 cgcctgtctg tgtgagtgca tcggtcagaa gctgatgata gctgcccgtc tgttctttgg
     3121 tcagggaaaa aataatcgca aggtgtgtcg tgtcaaggaa cagaatccgg catggcacat
     3181 atttgacgat cagattccgg atcatctggt agctgctgcg gtagagtgtg atgtccgacg
     3241 atgcgacagc ggacgcactc tgcctggtat tctgcatttc cagatacgcc accgtgtagc
     3301 cggcggcagt gaagcggacg gacagaagat cgcactgtgt cgcaaattgt tcctctgtct
     3361 cgaatagatt tagaagcagc cggatataaa aattatcctg caataaggag agatcggaac
     3421 cggtttccgg gatggctgcc tgttttctct ctttttccag aatgccgaga gccttctgga
     3481 tggcagcgtt taacacatct tccgtcagct cgagctttac cagataatcc agcacctggt
     3541 aggagatcgc ctgttttacc agagcaaatt cctcatagct ggttaagaga ataaaaagag
     3601 gcggatgctc ttgggactga catttctcga tcagctgcat gccgtccata atcggcattt
     3661 tgatgtctgt aatcacaatt tccggatgct tttccagaat gatatcatat gcctgcctgc
     3721 cgtttgtggc agtgcctgca atttcaattc ccatgctctc ccagtttaac atggagcgga
     3781 tgccgatctg aaccagcggt tcgtcatcca cgataagaag ctgtaccata actgtaaccc
     3841 catttctttg ttgtaaaaat atctgtctgc atttagccga tgtcttcctg cggcgcaaac
     3901 ggaagcagga cggacacagt ggtaaattcg gaaagccggc tttccacacg caggccgtaa
     3961 tcttcgccga attcatactg cagccgcttg tgcacgttgc tgacgccgat ttccctgaaa
     4021 aattcagtct tttcgttctt gttctcggac aaaagctgtc ttgcctgttc ttccgtcatg
     4081 ccgacgccgt cgtcggtaat gtcgatctga agaatcgcgt catttttccg gaaaatgtga
     4141 atagtgatcg ttccgggtgt tccctttggc tcaatgccat gaaagataga attttccacg
     4201 accggctgta aggtgaagcg cggaatcaga cagcttaatg ccgcctcgtc atcgatcctg
     4261 tattctaagg agatggttcc tccataacgg tatttctgaa tcgtaaaata atcatccaga
     4321 agggagagtt ctgcaga
//
LOCUS       DBMDQJ010000125         4334 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7641_length_4334_cov_1.493503, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000125 DBMDQJ010000000
VERSION     DBMDQJ010000125.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7641_length_4334_cov_1.493503"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1365
                     /locus_tag="ACZO9R_03630"
     CDS             <1..1365
                     /locus_tag="ACZO9R_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HIR0912196.1"
                     /translation="SNTYTIEELNANLMDILDDCEKRAEVSFDGAVKLDLKSKVRNGR
                     LYVDQGIIAGCAGGGFENICDAADILKGASIGPDEFTLSVYPASTPIYMELVKNGAVA
                     TLMETGAIVKTAFCGPCFGAGDTPAINAFSIRHSTRNFPNREGSKVQKGQVASVALMD
                     ARSIAATAANKGYLTAATDVDVDFSKPKYHFDSAIYANRVFDSKGVADPSVEIQFGPN
                     IKDWPAMSALPENMVLKVVSEIHDPVTTTDELIPSGETSSFRSNPLGLAEFALSRKDP
                     QYVGRAKEIQKAQKAVEEGTCPVEAVEELRPVMDAIRAAFPEKTFGEEMKPGTLGFGS
                     TIFAVKPGDGSAREQAASCQKVLGGWANIANEYATKRYRSNLINWGMLPFVIPEGALP
                     FQNLDYLFLPDIRKAVEEKQSEITAYVVKGSELKEFKLGLGAMTDDEREIILKGCLIN
                     YYRG"
     gene            1601..>2024
                     /locus_tag="ACZO9R_03635"
     CDS             1601..>2024
                     /locus_tag="ACZO9R_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate/malate family dehydrogenase"
                     /protein_id="HIR0912197.1"
                     /translation="MMNPRKVAVIGCGFVGASIAFALMQKGLFSEMVLIDANHAKAEG
                     EAMDLSHGLPYTASMDIYAGDYKDVADCGLIIITAGANQKPGETRLDLIEKNVGILKS
                     IVPQITATAFEGILMIVANPVDVLTYAAQQISGYPVERV"
     assembly_gap    2025..2034
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2035..2561
                     /locus_tag="ACZO9R_03640"
     CDS             <2035..2561
                     /locus_tag="ACZO9R_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rossmann-fold NAD(P)-binding domain-containing
                     protein"
                     /protein_id="HIR0912198.1"
                     /translation="FGSGTVLDTARLKYQVGRHLDVDSRSVHAVIIGEHGDSELPVWS
                     GANVSGIDLNHFCELRGHFNHEASMERLYEEVRDSAYEIIERKGATYYGIAMAVARIA
                     ESIVKDEHAVLPVSVELQGEYGLSGLALSIPSVVGKNGIEKVLEIPLNEKEKNALLAS
                     ADQLKAVIEKLTLN"
     gene            2956..4287
                     /locus_tag="ACZO9R_03645"
     CDS             2956..4287
                     /locus_tag="ACZO9R_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR0912199.1"
                     /translation="MDSERRQTDNTSGDRNGGWNLKQYVVIALVVFITFCCCILFFFM
                     IYRYNGFADFWKKLTTILQPIIMGLVLAYLLNPVMKFLERHLMKLLEPRMKSKTKAKK
                     MSRGVAIAGAWVFLGGIVVLLIAAIVPSIIQSIQGIATGLPSEVKNIMDWSDEFFKGD
                     SEIVGVINDVLQKASDAVQKFLENDLLAQVQVYLTSIASGVIYTLKFLLNLVVGIIVS
                     IYVLASQETFAGQAKKIIYAMFKPVRANVIVETVRKSNEIFGGFISGKLLDSAIIGIL
                     AYVVLTIMRMPDTILLAVIIGVTNIIPFFGPFIGAIPSFLIVVLQNPLQGVYFLIFIF
                     ILQQIDGNIIGPKILGDSTGLSSFWVVFAILVFGGLWGFPGMLLGVPLMAVIYYVAQK
                     LVSYFLRKRGLVDDTGSYIHLMRIDKRTNELVYEEKTEKGTGEKIEESPEK"
BASE COUNT         1112 a          903 c         1290 g         1019 t
ORIGIN      
        1 agcaacacct acacgatcga ggagctgaac gcaaacctga tggatattct ggatgactgc
       61 gagaagcggg cagaggtaag ctttgacggt gcggtcaagc tcgacttaaa gagcaaggtc
      121 agaaacggca gactgtatgt ggatcagggt attatcgcag gctgcgcggg cggcggtttt
      181 gagaatatct gtgatgcggc ggacatctta aagggtgcgt cgatcggacc ggatgagttt
      241 acgctcagcg tttatccggc aagtacgccg atttatatgg aactggtgaa aaatggagcc
      301 gttgccacgc tcatggagac gggcgcgatt gtgaagacgg cattctgcgg accgtgcttt
      361 ggcgcaggag acacgccggc gatcaatgca ttttccatcc gccactcaac gagaaacttc
      421 ccgaaccgcg agggttccaa ggtgcagaag ggacaggtcg cttccgtggc gcttatggac
      481 gcgcgttcca tcgctgcgac ggcggcaaac aagggatatt tgacggcagc tacggacgtg
      541 gatgtagatt tttccaagcc gaagtatcat tttgacagtg cgatctatgc aaaccgtgtc
      601 ttcgacagca agggcgtggc agatccgtcc gtggagatcc agttcggacc gaacatcaag
      661 gactggccgg cgatgagtgc gcttccggag aatatggtat taaaagtagt atcggagatt
      721 catgatccgg taacgacgac agatgagctg ataccttccg gtgagacctc ttctttccgc
      781 tcgaacccgc ttggactggc agagtttgca ctctccagaa aagatccgca gtacgtggga
      841 cgtgctaagg agatccagaa ggcacagaaa gcagtcgagg agggcacatg tccggttgag
      901 gcagtcgaag aactgcgccc ggtaatggat gcgatccgtg cagcatttcc ggagaaaacg
      961 tttggcgaag agatgaagcc gggaacactt ggcttcggaa gtacgatttt cgcggtgaag
     1021 ccgggcgacg gttctgcgcg tgagcaggca gcgtcctgtc agaaggtgct tggcggctgg
     1081 gcaaacatcg ccaatgagta cgcgacaaag cgttaccgct cgaaccttat caactggggt
     1141 atgcttccgt ttgtcattcc ggagggagcg cttccgttcc agaatctgga ttatctgttt
     1201 ttgccggaca tccgcaaggc agtggaagaa aagcagagcg agattaccgc atacgtggtg
     1261 aagggttctg agttaaagga atttaagctg ggactgggtg cgatgacgga cgacgagaga
     1321 gagattatct taaaaggttg tctgatcaat tattatcgcg gataagcccg tttcgtggca
     1381 ataggatcag agagaacagg cagtgttaag aaatgcactg cctgtgtttt tacagcggag
     1441 tttgttttaa ctaaccaaaa agtttatttt gttgtaaata caacagacta attcatggta
     1501 ccatggtagg atttggaaga aatagaaaac acggggttgt gtaacaatag aacatagagg
     1561 aaaacgaccg cgtgtgtgaa atagaaagga gaaatgaata atgatgaatc caagaaaagt
     1621 tgcagtaatc ggatgtggtt ttgtgggggc gtccatcgcg tttgccctca tgcagaaagg
     1681 actgttttca gagatggtgc tcatagatgc caatcacgca aaagcggagg gggaggcgat
     1741 ggatttaagc cacggccttc cctacacggc gtcgatggac atctatgccg gggactataa
     1801 ggatgtggcg gactgcggtc tgatcattat taccgcggga gcgaatcaga aaccgggaga
     1861 gacgcgtctg gacctgatcg agaagaatgt cggtatatta aaatcgatcg ttccgcagat
     1921 cacggcgacg gcgttcgagg gaattttgat gatagttgcc aacccagtgg atgtgctgac
     1981 ctatgcggca cagcagatat ccggttatcc ggtggagaga gtctnnnnnn nnnntctttg
     2041 gctccggtac ggttctggat acggcgagac tcaaatatca ggttggcaga catctggatg
     2101 tggacagccg cagcgttcat gccgttatca tcggtgagca tggcgacagc gaacttccgg
     2161 tatggagcgg ggcgaatgtg tccggaatcg atctgaatca tttctgcgaa ctgcggggac
     2221 atttcaacca tgaggcatcc atggagcgcc tgtatgagga ggtgcgcgac agcgcctatg
     2281 agatcataga gcgcaagggt gcgacctact acggcattgc catggcggtt gcgagaattg
     2341 cagagagcat tgtgaaggat gagcatgccg tgcttccggt gtcggtggaa ctgcagggag
     2401 agtacggtct gtccggtctg gcactcagca tcccgtcggt ggtcggcaag aacggaatcg
     2461 agaaggtgct tgagattccg ctcaatgaga aggagaaaaa tgcacttctt gcgtcggcag
     2521 accagctgaa ggcagtcatt gaaaaattaa cattaaatta gcggcaaata aagaatagcg
     2581 attggaaaac ctccctgcat ggggtataat gtacgcatag gcaatgagca gtgccagccg
     2641 tgccgactgc aatataagac tgcctgcagg ctttatattg cagatggcgc ggatgatagg
     2701 gcgaacgccc gcgagtccga aagtgcgaag cgatttcgga ctcgcactgc gggaactttt
     2761 agcggcagcc ggatccgatg catgtgaacg cgccagcggg tccggaaaac cgtaggtttt
     2821 tcggaccttt tgccgtaggc gtaagaaaag ctgatagggc gaacgcccgc gagtccggaa
     2881 gagcgaagcg gtttcggacg tgcactgctg ggaattgcag cggcagacaa cagaggaaca
     2941 gcaaggaggt ttactatgga cagtgagaga cgacagacag acaatacttc cggcgacaga
     3001 aatggcggat ggaatttaaa gcaatatgta gtaatcgcgt tggtagtgtt tattacgttt
     3061 tgctgctgta tactgttttt ctttatgata tatcgttaca acgggtttgc ggatttctgg
     3121 aaaaagctga ccacaattct tcagccgatt attatggggc tggtgcttgc atatctgctc
     3181 aatccggtaa tgaagtttct ggaacgtcat ctgatgaagc tgttggaacc gcggatgaag
     3241 agcaaaacaa aagcaaaaaa aatgtcgcgc ggcgttgcga tcgcgggggc gtgggtgttc
     3301 ttagggggaa tcgttgtact gcttatcgcg gcgattgttc cttctatcat acagagtata
     3361 caggggatcg ccacggggct tccgagcgag gtcaagaata tcatggactg gtcggatgaa
     3421 ttcttcaagg gagattcgga gattgtaggt gttataaacg atgtgctgca aaaagcaagc
     3481 gatgccgtac agaagttcct tgaaaatgat ctgctggcgc aggttcaggt ttatctgacg
     3541 tcgatcgcaa gcggtgtgat ctatacgtta aagtttctgc tgaatctggt ggtcggcatc
     3601 attgtgtcca tttatgtgct ggcgagccag gagacgtttg caggacaggc gaagaaaatc
     3661 atttacgcca tgttcaagcc ggtacgtgcg aacgtgatag ttgagacggt gcgcaagagc
     3721 aacgaaatct ttggcggatt tatttccgga aaacttctgg attcagcgat tatcggtatt
     3781 cttgcctatg tggtgctcac tattatgaga atgccggata cgatcctgct tgcagtgatc
     3841 atcggcgtga ccaacattat tccgtttttc ggaccgttta tcggagcaat tccgtcattc
     3901 cttatcgttg tattgcagaa tccgctgcag ggagtgtact ttctcatttt catctttatt
     3961 ctccagcaga tcgacggcaa tattatcgga ccgaagatcc tcggcgattc tactgggctt
     4021 agttccttct gggtggtatt tgccattctg gtatttggcg ggctgtgggg attcccgggc
     4081 atgctgctcg gtgtgccgct catggcagta atctactatg tggcacagaa gctggtatct
     4141 tattttctgc gaaagcgggg tctggtggac gatacgggaa gctacattca tctgatgcgc
     4201 attgacaaga ggacaaacga gcttgtctat gaggaaaaaa cggagaaagg caccggggaa
     4261 aaaatcgaag aatcacctga aaaataatat gctgccctca tataggacaa tgaaatataa
     4321 aagtcctata tggg
//
LOCUS       DBMDQJ010000126         4332 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7643_length_4332_cov_1.081068, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000126 DBMDQJ010000000
VERSION     DBMDQJ010000126.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7643_length_4332_cov_1.081068"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /gene="hisF"
                     /locus_tag="ACZO9R_03650"
     CDS             complement(<1..680)
                     /gene="hisF"
                     /locus_tag="ACZO9R_03650"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR0912200.1"
                     /translation="MFTKRVIPCLDVNGGRVVKGVNFVNLRDAGDPVEIAAAYDKAGA
                     DELVFLDITATSDARNTVVDMVRRVAERVFIPFTVGGGIRTVDDFKALLREGADKIAI
                     NSSAINRPELISEAADKFGSQCVVVAIDARRRADGSGWNIYKNGGRIDVGLDAVEWAM
                     KADRLGAGEILLTSMDCDGTKAGYDIELTRQVAENVSIPVIASGGAGTKDHFYDALTK
                     GKADAVLAA"
     gene            complement(693..1298)
                     /gene="hisH"
                     /locus_tag="ACZO9R_03655"
     CDS             complement(693..1298)
                     /gene="hisH"
                     /locus_tag="ACZO9R_03655"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR0912201.1"
                     /translation="MIAIIDYDAGNLKSVEKAFLALGEECVVTRDHDSIRRADRVVLP
                     GVGSFGTAMQQLKKYELDQVIREVVQEDKPFLGICLGLQLLFEGSEESDGVEGLHLLD
                     GEIVKIPEAPGLKIPHIGWNSLDFPHEGRLFAGLKEQPYVYFVHSYYLKAKDPSIVKA
                     TTEYGVTIHASVEKGNLFACQFHPEKSSTVGLQILKNFAEI"
     gene            complement(1317..2867)
                     /locus_tag="ACZO9R_03660"
     CDS             complement(1317..2867)
                     /locus_tag="ACZO9R_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912202.1"
                     /translation="MSGAEEYLDGLLHSVEGGSPADTARETEPGDTADGLYAEKEQET
                     KKAGMSEEDDFLKSFEADTDLEGDADTFIRQFEDELDQTPGEERTQKTTEENDQFFKD
                     LDGILDSARDADESTGGAEAAETAHASEDGDLMVDTIGDFQDDDSDLMDLLQSEGDFP
                     DMSQMADPTAPAADTDAEMPDVTAMPETADEVQDEDDGKKKKRRKKKKKDEPDAGDEA
                     PAKEKKSLIKRIGDALFGEEEETEEAPADTGELQPEGLEQAPELDDLSDENLKLLQEL
                     EGTGAAEPEAEAEPEISEEEKKAQAKKEKAEKKAQAKKEKAEKKAQAKKAKAEKKAQK
                     AKKPKKPKEPDNTPPLPKKPVALIMVMAASFLALVLVGTNLFGYTLSFNNAKQAYELG
                     DYQAAFADVSGLTVKEADQETYQKYSIMANAAGEYQAYQTFMENGIYDMALDSLIRAV
                     GRCDKYSEEAAAYGCSTELAKIRDQAVAGLSGFGLTQERAQELYAERERDTYSQELYS
                     ILTAAGLG"
     gene            complement(2943..3851)
                     /locus_tag="ACZO9R_03665"
     CDS             complement(2943..3851)
                     /locus_tag="ACZO9R_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HIR0912203.1"
                     /translation="MDTNILLESGTNELEVLEFTLGNNHYGINVAKIREILTYQPVTP
                     VPNSHPSIEGIFMPRDTMISVVNLKRCLGMPDDAKTEGLFIITNFNKLNIAFHVDAVL
                     GIHRVSWEAIIKPDATINTEQNSASTGVIKMDDKLIIILDFEKIVTDISPETGLKMSD
                     MDDMGQRERCESPILIAEDSPLLSRLITDCLKKAGYTNLIVTMNGQEAWDKLQEFEKA
                     GNVREKVHCIITDIEMPLMDGHRLTKLVKTNDNMKKIPLIIFSSLVNEEMRIKGEQLG
                     ADAQLTKPEIGSLVSAIDDLIDKSMD"
     gene            complement(3943..>4332)
                     /locus_tag="ACZO9R_03670"
     CDS             complement(3943..>4332)
                     /locus_tag="ACZO9R_03670"
                     /inference="COORDINATES: protein motif:HMM:NF023043.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HIR0912204.1"
                     /translation="ELFGADGIQMTYAGAQLAPGYMEGDYYEMTPDAGKQYLVLSIDV
                     TNTGADGVGLDVLSVSPKFTVVLNQSVKSTAETTLLDTDFSTYQGTIPAGGSTRLVLV
                     FQVPDDVASADSVELYVQMDQMIQIIL"
BASE COUNT          869 a         1370 c          886 g         1207 t
ORIGIN      
        1 gccgcaagaa ccgcatccgc ttttcccttt gtcagtgcat cataaaaatg atccttcgtc
       61 ccggcgccgc cggaagcaat cacaggaatc gatacatttt ccgcgacctg tctggtcagt
      121 tcaatgtcat atcccgcttt cgtgccatcg cagtccatgc ttgtcagaag aatctctcct
      181 gcgccaagcc ggtctgcctt catcgcccac tcgacggcat ccagacccac atcaatccgt
      241 ccgccgttct tatagatatt ccacccggag ccgtccgcgc gtctccttgc gtcaatcgca
      301 accaccacgc attggctgcc gaatttgtct gccgcctccg agatcagttc cggtcggttg
      361 atcgcggaag aattgatcgc aatcttgtct gcgccctcgc ggagcagtgc cttaaaatca
      421 tccaccgtcc ttatgccgcc tccgaccgta aacggaatga acaccctctc cgccacgcgg
      481 cgcaccatat cgaccacggt atttctggcg tccgaggtag cggtaatgtc cagaaatacc
      541 agttcgtccg cacctgcctt atcgtaagct gccgcaatct ccaccggatc tccggcatcc
      601 ctcagattca caaaattaac accctttacc acacgcccgc cattcacatc cagacaggga
      661 atcactctct ttgtaaacat cgcatgcctc cgctatattt ccgcaaaatt cttcaatatc
      721 tgaagtccca ctgtgctgct cttctccggg tgaaactggc acgcaaacag gttgcccttc
      781 tccacggaag catgaatcgt cacgccgtac tctgtggtcg ccttcacaat cgacggatct
      841 ttcgctttca ggtagtagga atgtacaaaa tagacatacg gctgttcctt caatccggca
      901 aacaatcttc cttcatgcgg gaaatccagt gaattccagc cgatgtgcgg gatcttaagt
      961 cccggcgcct ccggaatctt cacgatctct ccgtccagca gatgaagtcc ctccacgccg
     1021 tcactctcct cgctgccctc aaacagcagc tgcagaccca gacagatgcc gagaaacggc
     1081 ttgtcttcct gcacaacctc gcggatcacc tgatccagct catatttttt cagctgctgc
     1141 attgccgttc caaaagaacc gacacccggc agtaccactc tgtctgcccg gcgtatgctg
     1201 tcatgatctc tcgtgaccac acattcctca cccagcgcga ggaacgcctt ttccacactt
     1261 tttaaattgc ctgcgtcata gtctataatt gctatcattc tctcactccg tcgatgtcat
     1321 ccaagtcctg ctgccgtcaa tatcgaatac agttcctgcg agtaagtatc ccgctctctc
     1381 tccgcgtaca gttcctgtgc gcgctcctgc gtaagtccaa atccggacag tcctgccacc
     1441 gcctggtctc tgatcttcgc gagttctgtg gagcagccgt aagccgcagc ctcctcgctg
     1501 tacttatcgc atcttccgac cgcgcggatg agggaatcca gtgccatgtc ataaataccg
     1561 ttttccataa aggtctggta tgcctgatat tctccggctg cattcgccat gatgctgtac
     1621 ttctggtagg tctcctgatc tgcctccttg accgtaagtc cgctcacatc cgcgaacgct
     1681 gcctgatagt cgcccagttc atacgcctgc ttggcattgt tgaagctcag ggtatatccg
     1741 aacagattcg ttcccaccaa gacaagtgca aggaaagacg ccgccatgac cataatcagc
     1801 gccaccggct tcttcggaag cggcggtgta ttgtccggct ccttcggctt cttcggcttc
     1861 ttggctttct gtgctttttt ctctgccttc gcctttttcg cctgtgcctt cttctctgct
     1921 ttctctttct tcgcctgcgc cttcttctct gctttttcct tctttgcctg cgctttcttc
     1981 tcttcttctg aaatctccgg ttccgcctct gcctccggct cggctgcccc cgttccttcc
     2041 agttcctgta agagctttaa attctcatcg gaaagatcat ccagctccgg tgcctgttct
     2101 aaaccttccg gctgtaattc cccggtgtcc gctggtgcct cctccgtctc ttcctcctca
     2161 ccaaacagtg cgtcgccgat tctcttgatc aggctctttt tctcctttgc aggagcctca
     2221 tccccggcat ctggctcatc ttttttcttc tttttgcggc gctttttctt tttcccgtcg
     2281 tcctcgtcct gcacctcatc cgcagtctcc ggcattgccg tcacgtccgg catttctgcg
     2341 tctgtgtctg cggccggagc agtgggatcc gccatctggc tcatatccgg gaaatcgccc
     2401 tccgactgca gcagatccat cagatctgaa tcgtcgtcct ggaaatcacc gatggtatcg
     2461 accataagat ctccgtcctc cgacgcatgg gcagtctccg ccgcttctgc accgccggtg
     2521 ctctcatccg catcccttgc gctgtctaaa atgccatcca gatccttgaa gaactgatcg
     2581 ttctcctccg tcgtcttctg tgttctttcc tctccaggcg tctgatccag ctcatcctcg
     2641 aactgtctga taaatgtgtc cgcatctcct tccagatctg tatccgcctc aaaggacttt
     2701 aaaaaatcgt cttcctcact cataccagct ttttttgtct cctgttcttt ttctgcataa
     2761 agcccgtctg ccgtatcgcc tggctccgtc tcacgggctg tgtcggcagg acttcctccc
     2821 tctacggagt gcagcagccc atccagatat tcctctgctc cgctcatacg atacctccat
     2881 cgacctataa cttaagcggg ggatgtgatc ctgctccatc acatcccctc ttcatttcat
     2941 tctcagtcca tgctcttgtc aatcaagtcg tcaatcgccg aaacaagact gccaatctcc
     3001 ggctttgtca gctgggcatc cgctccaagc tgttctccct tgatgcgcat ctcctcattt
     3061 acaagagatg agaaaataat caatggaatc ttcttcatat tgtcatttgt cttaaccagc
     3121 ttcgtaaggc ggtgtccgtc catcaacggc atctcaatat ctgtgataat gcagtgtact
     3181 ttctcacgca cattgcctgc tttttcaaac tcctgcagct tatcccatgc ctcctgaccg
     3241 ttcattgtca caatcaggtt cgtgtagcct gccttcttca ggcagtctgt gatcagtctg
     3301 ctaagaagcg gggaatcctc tgcgatcaaa atcggagatt cacagcgctc acgctgtccc
     3361 atgtcgtcca tatcggacat cttcagtcct gtctccgggc tgatgtccgt cacaatcttc
     3421 tcaaagtcaa ggatgatgat cagcttgtca tccatcttga tgacaccggt cgaggcgctg
     3481 ttctgctcgg tattgatcgt ggcatccggc ttgatgatcg cctcccagga aacacggtgg
     3541 ataccaagca cggcatctac gtgaaatgca atgtttaact tgttaaagtt cgtaatgata
     3601 aaaagtccct ctgtcttcgc atcatccggc attcccagac agcgcttcag atttaccaca
     3661 gagatcatcg tatcacgcgg catgaagatt ccttcgatac tcggatgtga atttgggaca
     3721 ggcgtaaccg gctgataggt taaaatctcc ctgatctttg caacattgat tccgtaatga
     3781 ttgtttccca aggtaaattc cagaacttcc agttcgtttg ttcccgattc caataatata
     3841 tttgtatcca ttcgtctctc tcctctcgcg taataccgca acgtatctat ctgacagcat
     3901 tatagcacat tggtacctga aatgcattgt ttttcatgcg ttttacagaa taatttgtat
     3961 catttgatcc atttgcacat acagttccac actgtctgcc gacgccacat catccggcac
     4021 ctggaataca agtaccaggc gggtgctccc tcccgcagga atcgtaccct gataggtgga
     4081 aaaatccgta tccagcaatg tggtctccgc cgtgctcttg accgactggt tcaatacaac
     4141 cgtgaacttc gggctgacac tcaaaacatc aagacccact ccgtccgccc cggtgttggt
     4201 cacatctatg gacagcacca gatactgttt tccggcatcc ggggtcatct cataataatc
     4261 accttccatg taccccggtg caagctgtgc gcccgcatag gtcatctgaa tgccgtcggc
     4321 tccgaacagt tc
//
LOCUS       DBMDQJ010000127         4328 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7652_length_4328_cov_1.521647, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000127 DBMDQJ010000000
VERSION     DBMDQJ010000127.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7652_length_4328_cov_1.521647"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            481..1614
                     /locus_tag="ACZO9R_03675"
                     /pseudo
     CDS             481..1614
                     /locus_tag="ACZO9R_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(1765..2646)
                     /locus_tag="ACZO9R_03680"
     CDS             complement(1765..2646)
                     /locus_tag="ACZO9R_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912205.1"
                     /translation="MERSRRIRSAVILEAALLFLMGAILWENGKLPQKSAVETTADGY
                     IKWVDFNVSYEALSRAYEYDISSYGEKQHLDWIDLLAYVGAKNGGDFGAGATEDMRTA
                     AEKILCGEDTMEHMTADMKMFSYYREAYGAVLGGMVGEYEIQEKEEGSYVKKYGLKAF
                     SPIAKGFEYSDYDDFGVSRSYGYQRQHLGHDMMGQVGTPIRTKSRKTLKSNVEMIAIE
                     NTGLYDGADQFVNFGITHGVHVVKQIAYPVIHKFFCLGGHCRPAVALLGGAKLLFKDG
                     LFFVQSFQFSNDILCRS"
     gene            complement(2718..3128)
                     /locus_tag="ACZO9R_03685"
     CDS             complement(2718..3128)
                     /locus_tag="ACZO9R_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR0912206.1"
                     /translation="MAGSGYATNSRRKILDFLKSNSDRTVTVADIDQYLKRNGDAVNI
                     TTIYRYLDKLATDGTVMKYVAEAGGKAVYQYVELGQHCEEHLHLKCVKCGCIIHLDCG
                     FMDEIAEHIQKDHGFQLQCKNSILYGICKDCRRE"
     gene            complement(3435..4289)
                     /locus_tag="ACZO9R_03690"
     CDS             complement(3435..4289)
                     /locus_tag="ACZO9R_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese transporter"
                     /protein_id="HIR0912207.1"
                     /translation="MLDVLLDTVLDSVRLLPFLFLTYLFMEALEHHTGSKLSRKIQSA
                     GKWGPVWGGLLGVIPQCGFSAAASSLFAGRVITVGTLVAIYLSASDEMLPILISESVP
                     VVTIVKILGSKVIIAVLSGLIVELVYVRLLKKQEKEMDIHVVCEEEHCHCEDGIVVSA
                     LKHTVKIFCFIFLISLVLNGVIGLIGEEALAGLFSGLPVVGELIAALVGLIPNCASSV
                     VITQLYLDGIIRPGAMMAGLLVNAGVGFLVLFRLNRDYKQNAAIIATMYALGVFWGVV
                     VEACGIVF"
BASE COUNT         1154 a         1205 c          899 g         1070 t
ORIGIN      
        1 tacaattagt aaaatccgaa tttttgaccc tcgcaattta aaaggtgtac ttgcaggtgt
       61 atcactttca gaagagagta tggaaaaaat caaccaaaaa gtaaaagaat tgtacacttt
      121 tcattagttt ttattagttt tagtaatatt tttattgact gcgagcataa tatgtcatat
      181 aataagttag cttagaaaat ttattttcta tgaatatcgc ctttagggca taaaagaaga
      241 tattaactta ttatgtgaga gcctcgtaga aatacggggc tctttacgtt attaagcaaa
      301 ataaaaccgc cccaccaata agcggagcgg caatgcaact gttctgaatg aacaatcgcc
      361 ctcaacaagc atattgtatc attcggagca gcccggcgca agcggaacac ccgttctttg
      421 ctggctgtta tttttatacc tatttttaca tacactgtac aggaacaaag gagtgatact
      481 atgcttagaa tcggaatcta tccacgaaaa tcggtctacc gggacaacag tgactcggtg
      541 caggtgcaga tcacggcgtg caaggactac gcccggctga tgttcaagga tcaggaactg
      601 gatttcagaa tctacgacaa ggacgagggg ttctccggga agaatacgca ccgcccttcc
      661 tttaccgaac tgatggcgga tgtcaaggcg gacgaactcg atatcatcat cgtctacaag
      721 ctggaccgta tcagccgaag cgtcaaggac ttctctgaga tctatgagac gctgcagcaa
      781 cacaatgtct ctttcctgtc ggtaaaagaa tcctttgaca cttctacgcc tatgggacgg
      841 accgttatgt acatcctctc ggcatttgca cagttggagc gcgagaacac cgcagagcgt
      901 gttgcagaca atatggcaag cctgggggaa gccgggaagt ggacgggcgg caccctgccg
      961 ccgggcatga cctccgtccg caagaaaata ggcgataaga cgcactccta tctcatcgtg
     1021 gacgagcagc ggatcaagac ggtaaagtat atcttctccc tcatgctctc cggcacgtcc
     1081 atcaccaagt tggaacatac gctgcggcag gagggaatcc gcacggaacg ggatgccttt
     1141 tacagctgca gcaagctata caacctcttc accaatccag tatactgcgc caacgatatg
     1201 gacgcgtact actactttct ggaaaaatgc tacaagcttc ctgatccggc actcttcgac
     1261 ggtaggcacg ggctgatcgg ctacggccgc accacgcagt ccaacggaac gaccgtcaga
     1321 aaagatacct actcgatcgc catcggcatt catgatccga tcctctccgg tgccgactgg
     1381 atcgccatcc agaaacgcct tggccagaac aaatccaccc ggtacgccaa gtacgaagcc
     1441 gggattctaa agggtacgct ccgctgcaag tgcggcgtga aaatgaatat ccgtacatat
     1501 aaaaagaacg gcagcctgtt ttcctattac tactgcccca aaatggcgcg tgagggagtg
     1561 tccgcctgcg ataccggcta tacgcgggtg gatgacattg acgaccgttt tttacaggaa
     1621 ttaaaaaaga tcaagctgtc tccggacagc attgaacttg aggaaaatgt ctcttacgtt
     1681 gataccggta aaatgcgcac ggatctgaaa aagacgaccg actccatccg gaatctgatg
     1741 aatgcgctct ccggcagcgc cgattcagct gcggcaaagt atatcattgc tgaactggaa
     1801 gctctggaca aagaaaaggc cgtccttgaa aagaagcttc gcaccgccga gcagcgcaac
     1861 cgccgggcgg cagtgtccgc cgagacaaaa gaatttgtgt atcaccggat atgcaatctg
     1921 cttgacaaca tggactccat gagttatacc gaaattaacg aactgatccg ctccatcgta
     1981 aagtcctgtg ttttcgatgg cgatcatctc gacgttactt tttaacgtct ttctactttt
     2041 cgttctgatt ggggttccga cctgccccat catatcatgt cccagatgtt gtctttgata
     2101 tccatagctt cttgacaccc cgaaatcatc ataatcactg tactcaaacc cttttgcgat
     2161 cggtgaaaat gctttcagac catacttttt tacatatgac ccctcctcct tttcctggat
     2221 ctcatattca ccgaccatac cgccaagaac cgctccgtat gcctccctgt aataggaaaa
     2281 catcttcata tctgcggtca tatgctccat cgtatcctcc ccgcagagaa ttttttcagc
     2341 agcggtacgc atgtcctccg ttgctccggc tccaaaatct ccgccgttct ttgccccgac
     2401 gtacgccaga agatcaatcc agtcaagatg ctgcttttct ccgtaactgc ttatgtcata
     2461 ctcgtatgcc ctggacaatg cctcatacga cacattaaaa tctacccatt tgatatagcc
     2521 gtccgccgtt gtttccacgg ctgatttttg cggcagcttt ccattctccc agaggattgc
     2581 tcccatcaaa aaaagcaatg ccgcctcaag gatcacggcc gagcggatcc gcctgcttct
     2641 ctccattcct ttctgttccg ttttccattc ctcactgtca tggtcgtttt gttcattata
     2701 taaaaaaaga ctgcagacta ttcccgtctg caatctttac agataccgta taatatagaa
     2761 tttttgcact gtagctgaaa tccgtggtcc ttttggatgt gctctgcaat ctcatccata
     2821 aatccgcagt cgagatgaat gatgcatcca catttgacac atttcagatg aagatgctcc
     2881 tcgcagtgct gtcccagctc cacgtactga tacactgcct ttcctcccgc ctccgccaca
     2941 tatttcatga cggtgccgtc tgtggcaagc ttatccagat aacggtaaat cgtcgtgata
     3001 ttgacggcat cgccgtttcg cttcagatat tgatcgatat ccgccaccgt cacggtccgg
     3061 tcactgttgc tttttaaaaa atcaagaatc tttctgcggc tgtttgtcgc atatccactc
     3121 cctgccatct catccctccg caactgcatc tatttccatt atatcatctt ttctgcgccg
     3181 acttctgcgc atccgaagtt ccgcaggaac ttctaatatg aaatcgtcag agttcattat
     3241 atcagctacg cttcggcaaa agtccggaaa aactccgttt tcccggactc gcgggcgcgt
     3301 tcacatggaa catatccgcc cgactcatgc aagcatgcgc gtccgtcttt gctccatgct
     3361 cacttttgcc ttcgcgaaca ttatatcaca tttttgcgct caggcaaaag tctgtgaaac
     3421 catatccccg tctcctaaaa tacaattcca catgcttcaa ccacaacacc ccagaacaca
     3481 ccgagcgcat acatggtggc gatgatggcc gcattctgct tatagtcgcg gttcagccgg
     3541 aacagcacca gaaatcccac accggcattc accaaaagtc ctgccatcat ggcgcccggc
     3601 cggataattc catccaggta caactgtgtg attacaacgg acgacgcaca gttcgggatc
     3661 agccccacga gggcagcaat cagctctccc acaaccggaa gtccggaaaa cagtcccgcc
     3721 agcgcttcct ctccgatcaa cccgatcaca ccgtttaata ccagtgaaat caggaaaata
     3781 aaacagaaga tctttaccgt atgcttaagc gcggaaacca caatcccatc ctcgcaatgg
     3841 cagtgctcct cctcacacac gacatgaatg tccatctcct tctcctgttt cttcaagagt
     3901 ctcacatata ccagttccac aatcagaccg gacagcacag caatgattac cttactgcca
     3961 agaattttca caatcgtcac taccggcacc gactccgaaa tcagaatcgg aagcatctca
     4021 tccgaagcgg acaaatagat agcgaccaga gttccgaccg tgatgactct tccggcaaaa
     4081 aggctcgatg ccgctgccga aaacccgcac tgcggaatca caccgagcag accgccccac
     4141 acaggtcccc attttccggc ggactgtatc ttccggctca gcttgcttcc cgtatgatgt
     4201 tccaatgcct ccataaaaag ataagtcaaa aataaaaatg gcaaaagcct gacactgtca
     4261 agcactgtat ccaataatac atccagcata aaatcattcc tcccgggcgc tgtcatagcg
     4321 ctttccaa
//
LOCUS       DBMDQJ010000128         4328 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7653_length_4328_cov_1.495387, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000128 DBMDQJ010000000
VERSION     DBMDQJ010000128.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7653_length_4328_cov_1.495387"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            490..1956
                     /gene="trpE"
                     /locus_tag="ACZO9R_03695"
     CDS             490..1956
                     /gene="trpE"
                     /locus_tag="ACZO9R_03695"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HIR0912208.1"
                     /translation="MKRIYKVYKKTVSIVNETPTAMYENLVGSRKGFLLESYDKNYDR
                     YTFLGEPEELISSEGESLVITKKSGEREVRRGNPLALLKEYYDEFEIHKENDELNFSG
                     GLVGNVGYDFIRYSEKLPEKNPDEIGIETIQLMLMKAFIVMDYVAETLTAVVLEEETE
                     EGRKRAGVTAEELIRRAMHPEQIEGGCDSRCEADTGHGMKEAAQPEGGKRDGVIRKKS
                     DTLAAYSAKVEKIRHYIREGHIFQTVLSQRWTVETGRDGFSLYKELRELNPSPYLYYF
                     QFGDFEVIGSSPEMLVKQQGKRVFTCPIAGTRPRGKTVEEDEALHRELLADEKERAEH
                     VMLVDLARNDMGRISEFGTVKVTDFMNVRNYSHVMHIVSMVEGRKKGSYHPFDLLASF
                     LPAGTLSGAPKIRAMEIIEELEEVRRGLYGGATGYVDFSGDMDFCITIRTMIKKGREV
                     YLQAGAGIVADSVPEKEYQECCNKVMALARTLLEEENL"
     gene            1953..2522
                     /locus_tag="ACZO9R_03700"
     CDS             1953..2522
                     /locus_tag="ACZO9R_03700"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR0912209.1"
                     /translation="MILLIDNYDSFTYNLYQYIGTFTPDIRVVRNDAITIGEIEDMQP
                     ERIVLSPGPKSPKEAGICMDVVKHFYNKIPILGICLGHQCIGEAFGGTVSYAKTLFHG
                     KQSEVTHCLDGVFTGIDTPVKVARYHSLAVLRDDLPECLKVTAQTADGEIMAMRHKEY
                     PVVGLQFHPESIYTEHGKRMIENFLSGVI"
     gene            2537..3310
                     /gene="trpC"
                     /locus_tag="ACZO9R_03705"
     CDS             2537..3310
                     /gene="trpC"
                     /locus_tag="ACZO9R_03705"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR0912210.1"
                     /translation="MILDQIVSDKKRRLPEHKRRIPEQEMRKLAEAYESSHHDFKGAL
                     EGDVISIIGEFKKASPSLGKIKSKINLLDRVEEYNASVDAISCLTEEDHFDGSTAYLE
                     QIRQMTDLPILRKDFMIDPYQFYEARAIGADAVLLIAAILDDVQMHEFYQLAGELGLD
                     ALVEVHDEAEMERALKLDAKIIGVNNRNLKDFSIDLQTTERLSRMVPQEKLLVAESGI
                     VSDEDVEFLKACGVNAFLIGRAFMEAEHPGEVAARWKQR"
     gene            3323..3997
                     /locus_tag="ACZO9R_03710"
     CDS             3323..3997
                     /locus_tag="ACZO9R_03710"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012900.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR0912211.1"
                     /translation="MTKIKICGITGEDEIAALNETGVDYAGFVLYEKSKRYVTVQKAR
                     QLFEKLNKGIRKVAVTVAPERALIEDIGRAGFDLLQVHGVDAAEAERIAAQTKLPVWL
                     AVNLRDPAEVRRWRREEYHRIAGIVLDAGEYGSGKTFGWEQNGQDEMCCAVRAFREEL
                     LAEGRTFVLAGGLNAENVAEGIRIFAPDVADVSSGVETLCDGMRAKSIIRIKEFKKSV
                     QKTEGV"
     gene            4049..>4328
                     /locus_tag="ACZO9R_03715"
     CDS             4049..>4328
                     /locus_tag="ACZO9R_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR0912212.1"
                     /translation="MNRKAYYGAFGGQYVAESLMNTLEELDAAFEEAIRDPEFLEQYH
                     YYLKQYVGRETPLYYAERLSEKYGMKIYLKREDLNHTGAHKINNVIGQI"
BASE COUNT         1197 a          872 c         1360 g          899 t
ORIGIN      
        1 gggagcaggc aggatagtat ggatggaatg gatttgcgag agcaaagtga aaggcggata
       61 acagcagccg agtagcggaa gccgtgtcct cacgttacag gggtacgata tagagaacat
      121 ctgtatctga gagtctatac atgattggtg gcggatattt ccagtttgga ggtatccgct
      181 ttttgtatct tctgtcagat gagtccggtg aggacggctg ataaaaagag agaagctaag
      241 catggcaaga gaagaagggt ggcaccacga gacattcgtc cctgatccgg tttaccgggt
      301 cagggatttt ttttaattca tgggaaatta cgtccaatga attaaaaagc cctccgggca
      361 ggatcgcact gcgtcagagc ggaattatgt cgctagagca acccgccgca ggcggagaat
      421 cctgcaaagc aggattcttt cttgatggga cgcattttct atgaattaaa agaagaaaga
      481 ggaggattta tgaagcgcat ctataaggtc tacaagaaaa cagtcagcat tgtgaacgag
      541 acgccgacgg cgatgtacga gaatctggtc ggaagcagaa agggatttct gctcgagagc
      601 tatgacaaga actatgaccg gtatacgttt ttgggggaac cggaggaact gatctcctcg
      661 gagggggagt cgcttgtcat cacaaaaaag agcggggagc gcgaggtgcg caggggcaat
      721 ccgttagcgt tgttaaaaga atattatgat gaatttgaga ttcataagga gaatgatgaa
      781 ctgaattttt ctggcggact ggtgggcaat gtcggctacg attttatccg ctactcggaa
      841 aaactgccgg agaagaatcc ggatgagatc gggattgaga cgattcagct gatgttgatg
      901 aaagcgttta tcgtgatgga ctatgtggcg gagacgctga ccgcggttgt cttagaggag
      961 gagacggagg aaggaagaaa gcgtgccggc gtgacggcgg aagagctgat ccggcgcgcc
     1021 atgcatccgg agcagataga gggaggatgt gacagcaggt gtgaagcgga caccgggcac
     1081 gggatgaaag aggcggcgca gccggagggc ggaaaaaggg acggcgtcat ccgcaaaaag
     1141 tcggatacgc ttgccgctta cagcgcgaag gtggagaaga tcagacacta tatcagagaa
     1201 ggacatatct ttcagacggt actctcccag cgctggacgg tggagaccgg acgcgacggt
     1261 ttttctctct ataaggagct tcgcgagttg aatccgtcgc cgtatcttta ttacttccag
     1321 ttcggtgatt ttgaggtgat cggaagttcg ccggagatgt tagtaaaaca gcagggcaag
     1381 cgtgtgttta cctgcccgat cgcagggacc agaccgagag gaaagacagt ggaggaggat
     1441 gaagcactcc acagagaact tctggcagat gagaaagagc gtgccgagca tgtgatgctg
     1501 gtggatctgg cgcgaaacga tatggggcgc atctcggaat tcggaacagt caaggtcacg
     1561 gattttatga atgtgcggaa ttattcccat gtcatgcaca tcgtatccat ggtggaggga
     1621 aggaaaaagg gcagctatca tccgtttgat ctgctggcat ccttcctgcc ggcaggaacg
     1681 ttaagcggtg cacccaagat ccgggcgatg gagatcattg aggaattgga ggaggtacgg
     1741 cgcgggctct acggcggtgc gaccggttat gtcgattttt ccggcgatat ggatttctgc
     1801 atcacgatcc gcacgatgat caagaagggc agagaggtct atctgcaggc gggtgcggga
     1861 atcgtcgcgg actccgtgcc ggaaaaagaa tatcaggaat gctgcaacaa agtgatggcg
     1921 cttgcaagaa cacttctgga ggaggaaaac ttatgattct tttgattgac aactatgatt
     1981 cctttaccta taacctctat caatatatcg gaaccttcac gccggacatc cgggtggtac
     2041 gcaacgatgc cattacgatc ggggagatag aagatatgca gccggagcgc attgtgcttt
     2101 caccgggacc taaaagccca aaagaggcgg gaatctgcat ggatgtcgta aaacatttct
     2161 acaataaaat accgattctt ggcatctgtc tcggacatca gtgcatcggt gaggcgttcg
     2221 gaggaaccgt gagctatgcg aagacattat tccacggaaa acagtccgag gtcacacact
     2281 gtctggacgg cgtctttacc gggatcgaca cgccggttaa ggtggcaagg tatcattccc
     2341 tggcggtgct aagagacgat ctgccggagt gcctgaaagt caccgcgcag accgcggacg
     2401 gcgagattat ggcgatgcgg cacaaagagt atccggtggt cggtctccag ttccaccccg
     2461 agtccatcta cacggaacac ggaaagcgga tgatcgagaa tttcctgtcg ggtgtcattt
     2521 aagggaggag cagcgtatga tactggatca gattgtgagc gataaaaaaa gaagactgcc
     2581 ggaacataag agacggatac cggagcagga gatgcgaaag ctggcggagg cgtatgagag
     2641 cagccatcat gattttaagg gagcgcttga gggagatgtc atttccatca tcggagagtt
     2701 caaaaaggcg tccccaagtc tcggtaaaat taaaagcaag attaacttat tagatagagt
     2761 tgaggaatac aatgcttctg tggatgcgat ctcctgcctg acggaggagg atcactttga
     2821 cggaagcacg gcgtacttag aacagatccg gcagatgacc gatcttccga tcctgcgcaa
     2881 ggatttcatg attgacccgt accagttcta tgaggcacgt gcaatcggtg cagacgcggt
     2941 gcttctcatt gcggcaatct tagacgatgt gcagatgcat gaattctacc agcttgccgg
     3001 ggagctgggg ctggatgcgt tagtcgaggt acacgacgag gcggagatgg agcgggcgtt
     3061 aaagttggat gcaaagatca tcggcgtcaa taaccgcaat ctgaaggatt tttccattga
     3121 cctgcaaaca acggagcgat taagccgcat ggtgccgcag gagaagcttc tggtggcgga
     3181 aagcggcatt gtatcggatg aggatgtgga gttcttaaaa gcatgcggtg tgaatgcatt
     3241 tctgatcggg agagccttta tggaggcgga gcacccggga gaggtggcag cgcgctggaa
     3301 gcagcggtag ggaggatgga cattgacgaa gatcaagatc tgcggcatca caggagagga
     3361 tgagatagca gcgctcaatg agacaggtgt ggattacgcc ggattcgtgc tgtacgagaa
     3421 aagtaaacgt tatgtcacag tgcagaaagc aagacaacta tttgaaaaat taaataaagg
     3481 tataagaaaa gtagccgtga cggtcgcgcc ggagagagcg ttgatcgagg acatcgggcg
     3541 ggcaggattt gatctgctgc aggtgcatgg cgtggatgcc gcagaggcgg agcggatcgc
     3601 agcgcagacg aagctgccgg tgtggctggc agtcaacctc cgtgatcctg ccgaggttcg
     3661 ccgatggcgc agagaagaat atcaccggat tgccgggatt gtgctcgatg ccggggaata
     3721 cggcagcgga aagacattcg gctgggaaca gaacggacag gatgagatgt gctgtgccgt
     3781 gcgtgcgttc cgggaggaac tgctggcaga ggggcgtact tttgtgctgg caggcggact
     3841 taacgcagag aatgtggcgg agggaatcag aatctttgcc cccgatgtcg cagatgtcag
     3901 cagcggcgtg gaaacgctgt gtgacggaat gagagcaaaa agtataatca ggattaaaga
     3961 atttaaaaaa tcagtacaaa aaacagaagg cgtctgaaca ggaagaagaa ggcgcggcgg
     4021 aacgtgatca ggaaaggaaa gacgagacat gaacaggaaa gcatattatg gagcatttgg
     4081 cggacagtat gtggcggagt cgctgatgaa tactttagag gaactggatg cggcattcga
     4141 ggaagcaatc cgtgatccgg aatttctcga acaataccac tactatctga aacagtatgt
     4201 tgggagagag accccgttat attacgcgga gcgtctcagt gaaaagtacg gcatgaaaat
     4261 atacttaaag cgcgaggatt taaatcacac cggcgcacac aagattaaca atgtgatcgg
     4321 acagatcc
//
LOCUS       DBMDQJ010000129         4310 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7685_length_4310_cov_1.518175, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000129 DBMDQJ010000000
VERSION     DBMDQJ010000129.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4310)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7685_length_4310_cov_1.518175"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="ACZO9R_03720"
     CDS             complement(<1..827)
                     /locus_tag="ACZO9R_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004842869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR0912213.1"
                     /translation="MVRMKMKKKIDIEIIDKKQIPQSEPERQYYFMDIAKNYVEQLSA
                     KKGSPLTFHVTTFGCQMNARDSEKLVGILERIGYVETDTEEADFVIYNTCTVRENANN
                     KVYGRLGVLQNYKKKNPHMLIGLCGCMMQETVVVEKLKESYRFVDLIFGTHNIYKFAE
                     LIVTAMESDSMTIDIWKDTDQIVEDLPVERKYSFKSGVNIMFGCNNFCSYCIVPYVRG
                     RERSREPKEIIREIEQLVADGVVEVMLLGQNVNSYGKNLKEPVTFAQLLEEVEKIEG"
     gene            complement(828..2111)
                     /locus_tag="ACZO9R_03725"
     CDS             complement(828..2111)
                     /locus_tag="ACZO9R_03725"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR0912214.1"
                     /translation="MELTELCKSAKEVSRQIGNLDTNTKNEVLRAAAEAIVAHTEEII
                     RENEKDLARGRENHMPEGLLDRLMLNPSRMEQMAEGLRQVADLADPIGEVLSMKKRPN
                     GLMIGKKRVPLGVVGMIYEARPNVTVDAFALCFKTGNAVILKGGSDAISSNIVIVSVL
                     QEVLEAHGIPRAAVSLIEDTSRETAAAFMKMNEYVDVLIPRGGAGLIRAVVNQATIPV
                     IETGTGNCHIYVDESADFDMAIRIILNAKTQRIGVCNACESLVIHEKIVDAFMPKLAA
                     ALQQKDVVIHADEKSLAAAERREAANGGELKKELLSAATEEDWGREYLDYMISVKTVS
                     SLDEAIAHINRYNTGHSEAIITENYTNAQRFLDEIDAACVYVNASTRFSDGFEFGFGA
                     EIGISTQKLHARGPMGLDALTSTKYIIYGNGQTRP"
     gene            complement(2131..2775)
                     /locus_tag="ACZO9R_03730"
     CDS             complement(2131..2775)
                     /locus_tag="ACZO9R_03730"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR0912215.1"
                     /translation="MEKRQDFPDAALTQEKARQQIRARHKAWRMELPQEEVQEKSRKI
                     CERLAASDWYHNSAVIYGYYPLGAEADCLPFLAQALSDGKTVALPVTSKERLQRDCGR
                     EKRMQDTHRMEFYRIRELSRVREGAFHVMEPDESCKRIGEEDAVVLVPGLVFDRKGNR
                     YGYGGGYYDRYFARFPKLSLMAVAFEGQIEEQLAVLPTDVRMHRIYTEEREYRI"
     gene            complement(2750..2974)
                     /locus_tag="ACZO9R_03735"
     CDS             complement(2750..2974)
                     /locus_tag="ACZO9R_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HIR0912216.1"
                     /translation="MNQEQIDRINALYRKSKAEGLTEEEKKEQALLRKQYVADVKKNL
                     AAQLNNIDMVNPDGSVENLGDKYGKKTGLS"
     gene            complement(2996..3847)
                     /gene="proB"
                     /locus_tag="ACZO9R_03740"
     CDS             complement(2996..3847)
                     /gene="proB"
                     /locus_tag="ACZO9R_03740"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR0912217.1"
                     /translation="MEQKHFHFNEKKRVVVKIGSSSLNYGETGNLNFTKLEHLVRELC
                     NLRNRGMDVCLVSSGAIAVGRQTLGMDERPKDISTKQACAAVGQARLMMIYQRLFAEY
                     NQVAGQVLMTKNTMVNPVSRENAKNTFDELFRLGAIPIVNENDTVSTYEMQFGDNDTL
                     SAIVASLIGADLLILLSDIDGLFTDDPHKNPDAKLIEVVEEMDASIYGMAKSSTGSDV
                     GTGGMATKLTAAKIATLSGTDMIIANGRDVCILHHIFDDSFKGTIFQAKKKESFRIED
                     FILETIG"
     gene            4026..>4310
                     /locus_tag="ACZO9R_03745"
     CDS             4026..>4310
                     /locus_tag="ACZO9R_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR0912218.1"
                     /translation="MMRKKKVLSCLLVPLLSANLLLSSCSRTADEPVTKSGFYFNTVI
                     SVSIYEKGSEELLDDCFALAQKYEGYFSNTIPDSDISKINDAGGAPVTVHD"
BASE COUNT          959 a         1295 c          885 g         1171 t
ORIGIN      
        1 aacccctcaa tcttctccac ttcctctaaa agctgcgcaa acgtcaccgg ctccttcaga
       61 ttcttgccgt aggaattgac attctgtccg agcagcatga cctcgacaac gccgtccgcc
      121 acgagctgtt cgatctcacg gatgatctct ttcggctccc tgctgcgctc ccttccgcgc
      181 acatacggca cgatacagta actgcagaaa ttattgcatc cgaacatgat attgacgccg
      241 gatttaaacg aatacttccg ctctaccggg agatcctcca cgatctggtc cgtgtccttc
      301 cagatatcaa tcgtcatgga atccgattcc atcgccgtca caatcagctc tgcaaatttg
      361 tagatattat gtgttccgaa gatcagatcc acaaagcggt agctttcttt taatttttca
      421 acaactaccg tctcctgcat catgcagccg cagagtccga tcagcatatg tggatttttc
      481 tttttataat tctggagtac cccgagtctt ccgtagactt tgttatttgc attttcacgc
      541 accgtgcagg tattgtaaat aacaaaatcc gcttcctccg tatccgtctc cacataaccg
      601 atgcgctcta agataccgac aagcttctca gaatcgcgcg cgttcatctg gcagccgaaa
      661 gttgtgacat gaaaggtcag cggactgcct ttttttgcag agagctgttc cacataattt
      721 ttagcaatgt ccataaagta atactgacgc tccggctcgc tctgcgggat ctgcttttta
      781 tcgattattt caatgtctat tttctttttc atcttcatcc ttaccatcta cggacgtgtc
      841 tgcccgtttc catatataat atattttgtg ctggtcagcg catccagtcc catcggtcct
      901 cttgcgtgaa gcttctgggt actgatgccg atctccgcgc caaatccaaa ttcaaatccg
      961 tctgaaaaac gcgtcgatgc gttgacatag acgcaggcag catcgatctc atccaggaac
     1021 cgctgcgcgt tcgtgtagtt ttctgtgatg attgcctccg aatgcccggt attgtagcgg
     1081 ttgatgtgtg cgatcgcctc gtcgagggac gacaccgtct tcacggagat catataatcg
     1141 agatactccc tgccccagtc ttcctccgtc gccgccgaca acagttcctt tttcagttct
     1201 ccaccatttg ccgcctcccg gcgctctgcc gctgcaagac ttttctcatc cgcatgaata
     1261 acaacatcct tctgctgcag tgccgccgca agctttggca taaatgcatc cacgattttc
     1321 tcatggatca cgagtgattc acaggcattg cacacaccga tccgctgggt ctttgcgttt
     1381 aaaataatac ggattgccat atcgaaatcc gcactctcat ccacatagat atgacagttg
     1441 ccggttcccg tctcaatcac aggaatcgtc gcctgattga cgacggcgcg gatcagccct
     1501 gctcctccgc gcggaatcag cacatccaca tattcgttca tcttcatgaa cgcagccgcc
     1561 gtctcccggc ttgtatcttc aatcagcgac actgccgcac gcggaatgcc atgtgcctcc
     1621 aaaacttcct gcagaaccga aacaatgacg atattggacg aaatggcgtc actgccgcct
     1681 tttaagatca ctgcgttgcc cgtcttaaaa cacagcgcaa acgcatccac cgtcacattc
     1741 ggtctcgcct catagatcat cccgaccaca ccgagcggaa cgcgcttttt accgatcata
     1801 agaccgttcg ggcgcttctt catggaaagg acttcgccga tcggatcagc aaggtctgca
     1861 acctgccgca gtccctccgc catctgctcc atccgggatg ggttgagcat cagacggtcc
     1921 aacagtccct cgggcatatg attctctctg ccgcgggcga gatccttctc attctcgcgg
     1981 ataatctctt cggtatgtgc cacaattgcc tctgccgctg cacgcagcac ctcgttcttc
     2041 gtattcgtgt ccagattgcc gatctgtctg ctcacttctt ttgcgctctt acaaagttct
     2101 gtcagttcca tatcctttcc atcctttctg tcagatccga tattcccgct cctccgtata
     2161 aatccgatgc atccgcacgt cggtgggaag caccgcaagc tgttcctcga tctgcccttc
     2221 aaacgctacc gccatgaggg acagcttcgg aaaccttgca aaatagcggt cataataacc
     2281 gccgccatac ccgtaccggt tccccttacg gtcaaagacc agtccgggca ccagcaccac
     2341 cgcatcctct tccccgatcc gtttgcagct ctcatccggc tccatcacat gaaaggcgcc
     2401 ctcccgcacc ctggaaagct ctctgatccg gtaaaactcc atccgatgtg tgtcctgcat
     2461 tctcttctcc cggccgcagt cccgctgcaa ccgctctttt gatgtaaccg gaagcgccac
     2521 cgtcttgccg tccgaaagtg cctgtgccag aaacggcaga cagtcagcct ccgcgccgag
     2581 cggataatag ccgtagatga cagcgctgtt atgataccag tcagatgccg ccagtctctc
     2641 acagatcttt ctgctctttt cctgcacttc ctcctgcggc agttccatcc gccatgcttt
     2701 gtgccgcgcc cttatctgct gacgcgcctt ctcctgtgtc agcgctgcgt caggaaagtc
     2761 ctgtcttttt tccatactta tctccaaggt tctccacgga accgtccgga ttcaccatat
     2821 cgatattgtt aagctgcgcc gccagatttt tcttcacatc cgcaacatac tgcttgcgca
     2881 gcagcgcctg ctcctttttt tcttcctccg tcagcccttc cgccttcgac ttgcggtata
     2941 acgcattgat tctgtcaatc tgttcctggt tcatagtgct ccttttcctg tattcttaac
     3001 cgatcgtctc caaaataaaa tcttcaatcc ggaaagattc tttcttcttt gcctgaaaaa
     3061 tggttccctt aaaggaatca tcaaatatat gatgcagaat gcagacatcc ctgccgttcg
     3121 caataatcat gtctgttccc gagagcgttg caatctttgc ggctgtcagt ttggtcgcca
     3181 tgccgccggt tccaacatca ctaccggtcg aacttttcgc catgccgtag atcgacgcat
     3241 ccatctcctc caccacttca atgagctttg catccggatt cttgtgcgga tcgtcggtaa
     3301 aaagcccatc gatatccgag agcaggatca aaagatccgc cccgatcaga gaagccacga
     3361 ttgccgagag cgtgtcgttg tcgccaaact gcatctcata agtagaaacc gtatcatttt
     3421 catttacaat cgggattgcg ccaagacgga acaattcgtc gaacgtattc ttcgcattct
     3481 ctctcgagac agggttcacc atcgtgttct tcgtcatcag cacctgccct gccacctgat
     3541 tgtattccgc aaaaagccgc tgatagatca tcatcagccg cgcctggccg accgccgcgc
     3601 atgcctgctt tgtcgaaatg tccttcggcc gctcatccat gccgagcgtc tgtctgccca
     3661 cggcaatcgc accggagctt accagacaca catccatccc gcggttccgc agattgcaga
     3721 gttctcttac cagatgctcc agcttggtaa aattcagatt gcccgtctcc ccgtaattca
     3781 gtgaggaaga tccgatcttg actacaactc tctttttctc attaaaatga aaatgctttt
     3841 gttccatctg tctaacgtcc cctttatatg taacattttc caatcattca tcccaaatgg
     3901 gcatagagtc tcatactgta ttactctatc atatcttcat caaaatggca aattgatgtt
     3961 ttcacattgt atgaacagaa agtttatggt acattggatg ggaaacatca gaaatggaga
     4021 tttttatgat gagaaaaaag aaggtactct cctgtcttct ggttcccctg ctctctgcaa
     4081 atctgctgct ctccagctgc agccgcacgg cggacgaacc ggttaccaag tcaggatttt
     4141 attttaacac cgtcatctcg gtctcgatct atgaaaaagg gagcgaggaa ctgctcgatg
     4201 actgttttgc ccttgcgcaa aagtacgagg gatatttttc caatacgatt ccggacagcg
     4261 atatttctaa aatcaacgat gcgggcggcg ctcccgtgac agtacacgac
//
LOCUS       DBMDQJ010000130         4292 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7715_length_4292_cov_1.806251, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000130 DBMDQJ010000000
VERSION     DBMDQJ010000130.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4292)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7715_length_4292_cov_1.806251"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(161..1852)
                     /locus_tag="ACZO9R_03750"
     CDS             complement(161..1852)
                     /locus_tag="ACZO9R_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR0912219.1"
                     /translation="MNGLVIILIGIVALGAGYLFYGRWLAKKWGIDPNAKTPAYTHED
                     GEDYVPSSRFTVFSHQFSSIAGAGPVTGPILASVFGWVPVLLWLIIGGLFFGAVQDFG
                     ALYASVKNEGKSMGMIIEKYIGKTGRKLFMLFCWLFTLLVIAAFTDMVAGTFVGKGVD
                     GMTAETSYANSAAASISMLFIVVAVVFGLIQKHVGKMNEVVKAVVAIALLVAMFAVGM
                     ALPICTTKSAWIYIVMAYLFLASVMPMWLLMQPRDYMTTFMLLGMIIGAVVGVLVAHP
                     AMQLNAFNGFTVNGSSLFPTLFVTIACGAVSGFHSLVSSGTSSKTISNEKDMSMVGYG
                     AMVVESLLGIVSLVVVGAVAVNGTKPDGTPFAIFSSGVAGFLEKLGLPVQVATVFMTM
                     CVSALALTSLDSVARIGRMSFQELFYGDTTDPAKMSPLQRVLTNKYFATVITLFFGYL
                     LTLGGYNNVWPLFGSANQLLAALVLIALAVFLKTTGRTGWTLYIPMFAMLAVTFTALI
                     QKTIALVKNIAGGQATFLVDGLQFIVAVLLMVLGVLVAFSCLKKLFGKNRAGQAA"
     misc_binding    complement(1964..2215)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2365..3021
                     /locus_tag="ACZO9R_03755"
     CDS             2365..3021
                     /locus_tag="ACZO9R_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR0912220.1"
                     /translation="MKQILCFGDSNTYGLIPGTKDRYDWDTRWSGRIGQRLWEDGCRI
                     VEEGLCGRTTVFDDPLRDGRRGTELLSVILETHKPVDVVVLMLGTNDCKTMYDTSAEV
                     IGRGVERLLDQIRSSTPDSKILLMSPIALGEGVGEAGYDPEFDESSVAVSKRLPEVYR
                     RIAHERGISFLAASAYAKPSEEDREHMNEEGHAALAEAVYAKLKEMLAGEKENAQIPA
                     "
     gene            3278..>4292
                     /locus_tag="ACZO9R_03760"
     CDS             3278..>4292
                     /locus_tag="ACZO9R_03760"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HIR0912221.1"
                     /translation="MAEKNLDFDTVIDRTHTNSLKYDFAVQRGKPKDVLPLWVADMDF
                     QVSSYIQEALAGSVAHGIFGYSEAAGSYFEAVAGWMGRHYGWNVEEPWLVKTPGIVFA
                     LAQAVRAFTEPGDAVLLQQPVYYPFSQVIRDNGRKIVDNTLVQGEDGRYRIDFADFEE
                     KIRSCKVKLFFLCNPHNPVGRVWTREELLRLGDICEKYGVIVVSDEIHADFVFEGSHQ
                     VLAGLKESYRKFVVTCTAPSKTFNLAGLQVSNIFIPDDALRRKFEQAVAASGYSQLNT
                     MGLVACEAAYRYGEEWYDAVKAYIRENLTYAQHFLEEQIPQVKAVKPEGTYLLWVDFL
                     RLHC"
BASE COUNT         1106 a         1024 c         1278 g          884 t
ORIGIN      
        1 atgccagcca cggcatggaa gaaatacgtg aattttgctt caaagaattt cccgccgtct
       61 gtttattctg gaactttttt gttctctagt tttctggtaa tattacttat cactccaaag
      121 aggtctatgc ttattgcggc aattccttat tacgccaata ttatgccgcc tgaccggcac
      181 ggttctttcc aaacagcttc tttaagcagc tgaaggcaac cagaacgccg agtaccatta
      241 aaagcacagc gacgataaac tgcagtccgt cgacgaggaa tgtcgcctga ccgccggcga
      301 tgttttttac cagagcgatc gtcttctgga tgagtgcggt gaacgtcacg gcgagcatgg
      361 cgaacatcgg aatgtagagc gtccagccgg tgcgtccggt tgtctttaag aagactgcca
      421 gtgcgatcag gacgagtgcg gcgagaagct ggttcgcgga accgaacagc ggccatacat
      481 tgttatatcc gccgagtgtc agcagatagc cgaagaacag ggtgatgacc gtggcaaaat
      541 atttatttgt cagcacgcgc tgcagcggag acatttttgc cggatcggtc gtatcgccgt
      601 agaacagttc ctggaaggac atacgcccga tacgtgcgac ggaatccaga gatgtcagcg
      661 caagtgcgga gacgcacatg gtcatgaaga ccgtggcaac ctgtaccgga agaccaagct
      721 tctcgaggaa accggcaacg ccggaggaga agatggcgaa cggagttccg tctggcttgg
      781 ttccgtttac cgcgacagca ccgacgacga caagggagac aatgcctaac agggactcaa
      841 caaccattgc gccgtagccg accatcgaca tatctttttc gttgctgatc gtcttggagg
      901 acgttcccga ggagaccagg ctgtggaaac cggagacggc accgcaggcg atcgtgacga
      961 acagcgttgg aaaaaggctg ctgccgttta cggtaaagcc gttaaaagca tttaactgca
     1021 tggcaggatg agcgacgaga acgccgacca ccgcgccgat gatcatgccg agcagcatga
     1081 acgttgtcat ataatcacgc ggctgcatga gaagccacat cggcatcacg gatgccagaa
     1141 acagatacgc catgacaatg tagatccagg cggatttggt ggtgcagatc ggaagtgcca
     1201 taccgactgc aaacatggca acgagcagcg cgatcgcaac aaccgctttt accacttcat
     1261 tcatcttgcc gacgtgcttc tggatcagac cgaagacaac ggcgacgacg ataaagagca
     1321 tggagatgga agcggcggcg gagttcgcgt aggaggtctc ggcggtcatg ccgtccacgc
     1381 cttttccgac aaacgtgccg gcaaccatgt ccgtgaacgc tgcgatgaca agcagggtga
     1441 acagccagca gaacagcatg aaaagcttcc ggccggtctt gccgatatat ttttcaataa
     1501 tcatacccat cgacttgccc tcatttttga ccgaggcata tagcgcaccg aaatcctgta
     1561 ccgcgccgaa aaacagaccg ccgatgatca gccacaaaag taccggcacc cagccgaata
     1621 cggaggctaa gatcggaccg gtgacagggc cggcgcctgc gatggaagaa aactgatgag
     1681 aaaacacagt aaatctggaa gacgggacgt aatcctcgcc atcctcatgg gtgtaagcgg
     1741 gagtcttggc attcggatca atgccccatt tctttgccag ccagcgccca tagaagagat
     1801 agccggcacc cagtgctacg attccgatta agataatcac taaaccattc attttttttc
     1861 ccgcccgaag gcggctcctt tctgatagat caacattggg caaagagaag gatggcattt
     1921 tctgtaaaac aaaaacgccc ccgtcccgct gcacgaatgt gcagccaaag acgaaggcgt
     1981 aagcttccgc gttaccactt tgcttgtcgt aaaaaacgac ctctcaatct catctttgcg
     2041 ggcgtaagcc tgcagcaata agcttccctg taacggggga atcccggtga cacctacttg
     2101 gttcagtgca ctgctcgcag aggatagttc ctgaaaccgc ataccgtctc gcaccgaccg
     2161 acggctctct gtgatgcggg gaatcagtaa accgtgttct gttccttgca tttgctcttt
     2221 gtttgattat gaagatagca ctgtgatgtg taaaagtcaa gtacaaattt ttaatgtaag
     2281 aatctggtaa agacggttga caaataaaaa aacataatat atagtattcc tatcagaaca
     2341 gtatgagaaa ggagcgcggc ttctatgaaa cagatattat gctttggaga ttcaaataca
     2401 tacggattga taccgggaac gaaggatcgc tatgactggg acacccgctg gagcggacgg
     2461 atcgggcagc ggctgtggga ggatggctgc cgcattgtcg aggaaggact gtgcggacgg
     2521 acgacggttt ttgatgatcc gttgcgggat ggcagacggg gaacggagct tttatctgtg
     2581 atcttagaga cgcataagcc ggttgatgtg gttgtgctga tgctcgggac gaacgactgc
     2641 aagacgatgt acgatacatc ggcggaggta atcgggagag gcgtggagcg gctgctcgac
     2701 cagatcagaa gcagcacccc ggacagtaag attcttttga tgtcgccgat cgcactcggc
     2761 gagggcgttg gcgaggcagg gtacgatccc gaatttgatg agagctccgt tgcggtgtca
     2821 aagcggctgc cggaggttta ccggcggatc gcgcatgagc ggggcatttc gtttctggcg
     2881 gcatccgcat atgcaaagcc aagtgaggaa gacagagagc acatgaatga ggaagggcat
     2941 gccgcgctgg cggaggcagt gtatgcaaag ttaaaggaaa tgctcgccgg agagaaagag
     3001 aacgcacaga tcccggcata gacgaatccg gcagggagag aggacaaaaa cggaatctta
     3061 cggcagtgtg cctgacagga taaaaagaga cgaaagaaaa cgcgcagcgg gaatggaagg
     3121 atgatggctg ctgaaccgcc cgggagcaga cggcataaga taacagcaga agacgctgct
     3181 gcgcggagaa ggagcgaaag gagtatgccg gctgaaccgc acgggatccg gcggcataag
     3241 ataacagcag gatacgctgc ggcgcggagg aagagacatg gcagagaaaa atctggattt
     3301 tgacacggtc attgaccgga cacataccaa ttcactcaaa tatgattttg cagtacagcg
     3361 gggaaagccg aaggatgtgc tgccgctttg ggttgcagac atggattttc aggtctcttc
     3421 ctatatacag gaggcactcg cgggcagcgt ggcgcacggc attttcggct acagcgaggc
     3481 ggccggcagc tactttgagg cggttgcggg ctggatggga cggcattacg gatggaacgt
     3541 ggaggaaccc tggctggtca agacgccggg gattgttttt gcgctggcgc aggcggtgcg
     3601 ggcgttcacg gagccgggcg acgcggtgct gctgcagcag ccggtctact atccgttttc
     3661 gcaggtgatc cgcgataacg gcaggaagat tgtggataat acgcttgtgc agggagagga
     3721 cggaagatac cggatcgatt ttgcggattt tgaagaaaaa atacgcagct gtaaggtaaa
     3781 gctctttttt ctctgcaacc cgcacaatcc ggtgggacgc gtgtggacaa gggaggagct
     3841 cctgcggctg ggcgacatct gtgaaaaata cggagtgatt gtggtcagcg atgaaatcca
     3901 cgcggatttc gtctttgagg gatcgcatca ggtactggcg ggtctcaaag aaagctaccg
     3961 gaaatttgtg gtgacctgca cggcgccgag caagacattt aaccttgcgg gacttcaggt
     4021 ctccaacatt tttatccctg atgatgcgct gcggcggaag ttcgagcagg cggtcgcggc
     4081 gtccggctac agccagctta acaccatggg gcttgtggct tgtgaggcgg cgtaccggta
     4141 tggagaggag tggtacgacg cggtgaaggc atatatccgt gaaaatctta cctatgcgca
     4201 gcattttctg gaagaacaga ttccgcaggt aaaggcagtg aagccggagg gaacgtacct
     4261 gctgtgggtg gatttcctcc ggcttcactg cc
//
LOCUS       DBMDQJ010000131         4269 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7760_length_4269_cov_1.058781, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000131 DBMDQJ010000000
VERSION     DBMDQJ010000131.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7760_length_4269_cov_1.058781"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="ACZO9R_03765"
     CDS             complement(<1..351)
                     /locus_tag="ACZO9R_03765"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR0912222.1"
                     /translation="MSWYLRLELPALITRMFILPFTPVFLFSIIRVTISHSYCSSYNK
                     SQTETRALQDFFTGGHSMQTVGVICEYNPFHLGHARQLSLIRQQLEPETAIVCLMSGN
                     YVQRGEPAIFDKRVR"
     gene            299..436
                     /locus_tag="ACZO9R_03770"
                     /pseudo
     CDS             299..436
                     /locus_tag="ACZO9R_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
     gene            complement(446..904)
                     /locus_tag="ACZO9R_03775"
                     /pseudo
     CDS             complement(446..904)
                     /locus_tag="ACZO9R_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(995..1540)
                     /locus_tag="ACZO9R_03780"
     CDS             complement(995..1540)
                     /locus_tag="ACZO9R_03780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0912223.1"
                     /translation="MDKARWLDGILEQEGITKPVIVGQSMGGYVGQAYAQTYPEKLKG
                     FVSIDSAPLQRKYMTKPELWLLKRMEIVYRCYPWKALLKSGTKGVALSEYGRNLMRDM
                     MATRSATQRLPVTASESLPRRWSGICRMKSNAPPCSSAANRTRRGLALDTIRRGMRTP
                     EFPWNGSKMLGTTPTPTSRKS"
     gene            complement(1648..2058)
                     /locus_tag="ACZO9R_03785"
     CDS             complement(1648..2058)
                     /locus_tag="ACZO9R_03785"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR0912224.1"
                     /translation="MVKPIMKDIFFLGQKSEKATRADLQVGKDLEDTLEANREGCVGM
                     AANMIGVKKRVIIVNMGLVDLVMFNPVLLKADTPYEAEEGCLSLVGVRKTTRYQNIEV
                     EYTDKNWNKKRIKLSGWYAQICQHELDHLDGIII"
     gene            complement(2614..2778)
                     /locus_tag="ACZO9R_03790"
     CDS             complement(2614..2778)
                     /locus_tag="ACZO9R_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912225.1"
                     /translation="MMDVVVRKSRAIRSIYEQDRALFLESEHGLIRIWPQTDRIIRVS
                     YTENGVFPEN"
     gene            complement(2953..>3148)
                     /locus_tag="ACZO9R_03795"
     CDS             complement(2953..>3148)
                     /locus_tag="ACZO9R_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013656307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HIR0912226.1"
                     /translation="RGYAYGSMEIRTSFEGSAVKTIPLHYSNIWRDIKTEIKLPEGAD
                     SLYFTYRGPGSASFKGFTLK"
     assembly_gap    3149..3158
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3159..4264)
                     /locus_tag="ACZO9R_03800"
     CDS             complement(<3159..4264)
                     /locus_tag="ACZO9R_03800"
                     /inference="COORDINATES: protein motif:HMM:NF016497.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HIR0912227.1"
                     /translation="MGDYVCWSADIHDLGNWRYEGVIYKRNEDPGNPDGEGCLYAPDV
                     TKGPDGRYYLYYVVSNLQVVSVAVCDEPAGKYTFLGYVHDQENRRIGEREGDEPQFDP
                     GVLTEGDKTYLYTGFCGRGDKSRHGAMGMLLGRDMLTVLEGPVIVAPGCEYSEGTGFE
                     GHEYFEAASIRKKDDLYLLIYSSVVMHELCYAYSKSPLEGFQYGGVLNSNVDLGIDTY
                     KPADRPAAFGANNHGSIVEMNGDWYIFYHRHTNGTWFSRQGCAEKLPIREDGLFGQAE
                     LTSCGLNGGPLAGEGYYPSCIACHLFTDRPVIYVGDSDKPEYVNGSFPKIVKEGWDGD
                     QNEGYITNMYDQCTAGFRYFDLSHVSEITLTIRG"
BASE COUNT         1022 a         1214 c          897 g         1126 t
ORIGIN      
        1 ccggacgcgc ttgtcaaaaa tggcaggttc tccacgctgg acatagtttc cgctcatcag
       61 gcagacaatg gccgtctccg gttccagctg ctgccgaatc aggctcagct gccgcgcgtg
      121 gccgagatga aagggattgt attcacagat cacgccgacc gtctgcatgg aatgacctcc
      181 ggtaaaaaaa tcttgtaaag cccttgtttc tgtttggctt ttgttgtatg atgaacaata
      241 gctatgtgat atagtaacac gaatgattga aaaaagaaat acaggagtga atggaagtat
      301 gaacattctg gttatcaatg ctggcagctc cagcctcaag taccagctca tgaacccgga
      361 caccggcgac gtcaccgcca aggggctgtg tgagcgcatc ggtctggacg gccgtctgac
      421 ccacaggcgg catggcggag cagtataaaa tcaggccttt cgtgcgttgg ggataatcaa
      481 gggcaaatcg gatcgcgacg ctgccgcctg ccgatgtggc cagcaggtgc accttgtcaa
      541 tgcccagctt gtccaacagc tccacatagg ccaccgcctg cctggcgggc gttccgtcgc
      601 cgaaaacgtc gcttcccagg tatccgaacc gtgagggagc gatgatccga tagtcggagc
      661 aaaagtcctt acaggtgtca taagcctggt catagccgcc gaaaatgccg tggacggata
      721 aaatcacctc gccctcaccc gaatcgacat aagttatatc gccgtagctt aatgccgccg
      781 tctgagcatt gtaagcggca agacgatttt tgctttccct caccgccacg ccgcatcgta
      841 tcccctggac aatgaaatac attgccaggg caaggaatac tccccccact atgtaactcc
      901 acattcgctt acaccttctc ttttcagtca ctgatttcgt aaaatcaagc ctccagacac
      961 ttcgcccgct gagccagctg ttcgatcagc cggtttacga ttttcggctg gtcggtgttg
     1021 gagttgtgcc cagcatcttt gagccattcc aggggaattc cggtgtcctc atgccacgcc
     1081 ttattgtatc taacgcaaga ccccgccttg tcctgttcgc cgcagatgag cagggcgggg
     1141 catttgattt catacggcaa atcccgctcc accgcctcgg caaggattcg gaagccgtga
     1201 ccggcaatct ttgcgtagcg cttctggtcg ccatcatgtc ccgcatcagg ttccttccgt
     1261 attccgacag ggcaacgccc tttgtcccgg actttaagag ggccttccac ggatagcagc
     1321 gatagactat ctccattcgt ttcagcagcc aaagctccgg ctttgtcata tactttcgtt
     1381 gcagcggcgc ggagtcgatg gacacaaagc ccttcagctt ttcggggtaa gtttgcgcat
     1441 acgcctgtcc cacatagccg cccatagact gccccacgat aacgggtttt gtgattccct
     1501 cctgctccag aattccgtcc agccaccttg ccttgtccat caaatcgaag tcaaaggcaa
     1561 agggccagga ggcggcgtgg ccgggggcgt cccacacaag agagccaata gggacaggta
     1621 aaatggcttc tttactgtcc cgctagttca aataataatt ccatccaaat gatccagctc
     1681 atgctgacat atctgcgcat accagccact aagctttatt ctcttcttat tccagttttt
     1741 gtcagtatac tcaacctcaa tattctgata gcgcgttgtt tttctcacac cgacaagtga
     1801 caaacatcct tcctctgcct catacggcgt atctgccttt aacaaaactg gattgaacat
     1861 gacaagatcc accaaaccca tgttgacaat gataactctc tttttgacac caatcatatt
     1921 cgcggccatg cctacacagc cctcgcgatt tgcctcaagt gtatcttcca gatcttttcc
     1981 tacctgcaag tcagcccttg ttgctttctc tgatttttgt ccaaggaaaa atatatcttt
     2041 cataattggc tttaccatag tacatctctc cttccaattt ttacttattg taccatcaaa
     2101 atctatgctt ttcaatacac gaccttttgt attgtcatat ccgccatttc ggtcatcaat
     2161 tcccttgatc agattcaaag aaaacaatcc acttcgatta cagtagacgg atcgtagagt
     2221 taaattaggt gatccagtgg gacaataaaa aagccatttt acctgtcccg ctggttcatt
     2281 aaattttaat gaaagacgtg tgtgataaag catcctgtca tattctctgg cagtttcaag
     2341 tactttctgt ttcactccat ccacggtttt gatctgtgcc gtctgtgcct ctccgtccac
     2401 cggccggaaa tacggaaatt cttccacctg tttgctcatc gcatccgctt ctgccagcaa
     2461 taaagaacca tctttttcct cataaattag tgaacctgtc tccctgttca cccgaactct
     2521 caattcttct gtctgaacca gaatctcctg tccctcggac cacgtccagg ctgccctctt
     2581 ttctttccct gacagatcct catattcttc tccctaattc tccggaaata ctccattttc
     2641 tgtataggat acacgaatga tacggtctgt ctgaggccag atccggatca gtccatgttc
     2701 cgattccaga aacagcgcac gatcctgctc ataaatgctg cggatcgctc tgctttttct
     2761 tacaactaca tccatcatag cagctgtcac tcctttcaca ggcagacagg ttccgtgact
     2821 cacacattgc tgccccctga ctttctccag tttatcatag ctgttcagag tcaactttca
     2881 aaatatcccg catttcgtca gtgtgacgta tccgccaaat aaagttggga attgcctggc
     2941 tctcaacagt tctcatttca atgtaaatcc cttaaagctg gcacttccag gtccccggta
     3001 ggtaaaataa agagaatctg ctccttccgg aagcttaatc tctgttttga tatctctcca
     3061 gatattagag tagtgcaggg ggatcgtttt taccgccgat ccctcaaatg aagttcggat
     3121 ctccatactg ccatatgcat atccacggnn nnnnnnnnta tccacggatc gtcagtgtaa
     3181 tctccgacac atgactcaga tcaaaataac ggaagcccgc agtacactga tcatacatat
     3241 tggtgatgta gccttcattc tggtctccgt cccatccttc tttaacgatc ttcggaaatg
     3301 agccattgac atattccggt ttatcactgt ctcccacata gatcaccggt cggtcggtaa
     3361 acagatggca tgcaatacag gagggataat aaccttctcc ggcaaggggc ccgccgttca
     3421 ggccgcagga ggtaagctct gcctgcccaa acagtccatc ttcacgaata ggcagttttt
     3481 ctgcacatcc ctgcctggag aaccaggtac cgttggtatg tctgtgataa aaaatatacc
     3541 agtccccgtt catttccacg atacttccgt gattgtttgc tccgaaggct gccggacggt
     3601 ctgccggttt atacgtatca attcccagat ccacattgct gttcagcact cctccatact
     3661 gaaatccctc cagcggagac ttgctgtacg cataacagag ttcatgcatc acaactgagg
     3721 aatagatcag aagatagaga tcgtccttct tccggatgga tgccgcttca aaatattcat
     3781 gaccctcgaa tccggtaccc tcactgtact cacagccggg ggccacgatc accggcccct
     3841 caaggacagt aagcatatcg cgaccgagca gcattcccat ggcaccgtgt ctggacttgt
     3901 ccccacgtcc gcagaagccg gtatacagat atgttttatc tccttctgtc agaacacccg
     3961 gatcaaactg gggctcatca ccctcacgct ctccgatccg gcggttttcc tgatcgtgaa
     4021 cgtagcccag gaaggtatac tttcccgccg gctcatcaca gactgccaca gagacaacct
     4081 gcagattgga gactacataa tacagataat atcttccgtc cggacctttt gtcacatccg
     4141 gggcgtagag gcatccttca ccgtcaggat ttccgggatc ctcatttctt ttgtatatga
     4201 ctccctcata tctccagttc cccagatcat ggatatcagc ggaccagcac acataatctc
     4261 ccatacaga
//
LOCUS       DBMDQJ010000132         4266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7764_length_4266_cov_1.359184, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000132 DBMDQJ010000000
VERSION     DBMDQJ010000132.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7764_length_4266_cov_1.359184"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /gene="lexA"
                     /locus_tag="ACZO9R_03805"
     CDS             <1..368
                     /gene="lexA"
                     /locus_tag="ACZO9R_03805"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR0912228.1"
                     /translation="VPILGNVAAGQPLLAVENIETYFPIPTEFLPNAETFMLRVKGES
                     MINVGIFDGDNVLVEKQSTARNGEMVVALVEDSATVKTFYKEDGYIRLQPENDTMDPI
                     IVPDCQILGKVIGIFRFLS"
     gene            complement(689..1036)
                     /gene="rsfS"
                     /locus_tag="ACZO9R_03810"
     CDS             complement(689..1036)
                     /gene="rsfS"
                     /locus_tag="ACZO9R_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR0912229.1"
                     /translation="MTSQEYCKIAVAALEDRKAEDVKIIDIREISPVADYFVIADGTN
                     QNQIQAMRDACEEALYKAGLKTKQIEGNSSSTWILMDYGDIIVHIFSKEDRLFYDLER
                     IWRDGKIVDVADL"
     gene            complement(1086..1673)
                     /gene="yqeK"
                     /locus_tag="ACZO9R_03815"
     CDS             complement(1086..1673)
                     /gene="yqeK"
                     /locus_tag="ACZO9R_03815"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HIR0912230.1"
                     /translation="MEQHEIRKRLKKELDKGRYEHTKGVMYTAGCMAMAYETSIEQAM
                     LAGLLHDCAKCIPDDEKLQICEEHDIPINAVEYESPFLLHAKLGAYLAETVYEVKDPE
                     ILHAIKVHTTGAPDMSTLDKIIYIADYIEPGRNKAADLPRVRKLAFEDLDACMAEILC
                     DTLKYRSRVGGPLDPSTQLAYEFYRQYQKEQTDPV"
     gene            complement(1676..2296)
                     /gene="nadD"
                     /locus_tag="ACZO9R_03820"
     CDS             complement(1676..2296)
                     /gene="nadD"
                     /locus_tag="ACZO9R_03820"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR0912231.1"
                     /translation="MRKIGILGGSFDPIHQGHLNIARSAYREFALDEVWFIPAGHSPN
                     KEEAEMTPAQVRAEMTQAAIKNDASFKLSRIEIESSGTSYTYKTLQHLKEQFPEDQFF
                     FIMGADSLDYFDKWRHPEIICQNATVLAAVRDTLELPQIEGKIRCIKALFPAEIYPLA
                     GGRTDVSSTAIRAQIRMTGECPAMLPGEVWELIKRYHLYGVSNLGE"
     gene            complement(2293..2589)
                     /gene="yhbY"
                     /locus_tag="ACZO9R_03825"
     CDS             complement(2293..2589)
                     /gene="yhbY"
                     /locus_tag="ACZO9R_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HIR0912232.1"
                     /translation="MELTSKQRAYLGGCASTLDPIFQIGKASLTPEIIQALDEALEKR
                     ELIKISVLKNCADDPREIAQVIAERTHSAVVKVIGKKIILFRQAKKNSKFELPA"
     gene            complement(2607..3890)
                     /gene="obgE"
                     /locus_tag="ACZO9R_03830"
     CDS             complement(2607..3890)
                     /gene="obgE"
                     /locus_tag="ACZO9R_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR0912233.1"
                     /translation="MFADRATIIIKSGKGGDGHVSFRREKYVPDGGPDGGDGGKGGDV
                     IFVVDEGLNTLTDYRHRRKFAAQPGEEGGKRNCHGKNGEDLILKVPEGTVIKDAESGK
                     VIADMSGENRRQVILRGGRGGLGNQHYATSTMQAPKYAQPGGDSIEIEVKLELKVIAD
                     VGLVGFPNVGKSTLLSRVTNAQPKIANYHFTTLQPNLGVVDLDGAKGFVIADIPGLIE
                     GASEGVGLGLEFLRHIERTKVMIHMVDAAGTEGRDPVADIIAINKELEAYDPALLKKP
                     QVIAANKMDAVYGDENEIIQKLKDTFEKDGIRVFPISAVSGKGLKELLYCVSELLDQC
                     SKEPVVYEPEFDPALRFFKDEPYTITVADDGAFVVEGPKIEKMLGYTNMDSEKGFLFF
                     QKFMKEQGILKDLEAQGIEDGDTVRMYGFEFDYYK"
     gene            complement(4031..>4266)
                     /gene="rpmA"
                     /locus_tag="ACZO9R_03835"
     CDS             complement(4031..>4266)
                     /gene="rpmA"
                     /locus_tag="ACZO9R_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008370482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR0912234.1"
                     /translation="TKNGRDSESKRLGAKRADGQFVKAGNILYRQRGTKIHPGVNVGI
                     GGDDTLFALTDGILRFERKGKDKKQASVYPVAE"
BASE COUNT          958 a         1206 c          923 g         1179 t
ORIGIN      
        1 atgtcccgat tctcgggaat gtggctgccg gtcagccgtt actcgcagtc gagaacattg
       61 agacctactt cccgattccg accgaatttc tcccgaacgc ggagacgttc atgttgaggg
      121 taaagggcga aagcatgatc aacgtcggca tcttcgacgg agacaatgtg ctggttgaga
      181 agcagtcgac cgcgcgcaac ggcgagatgg tcgtcgcgct ggtggaggat tccgctacag
      241 ttaagacctt ttataaggag gacggttata tccgccttca gccggagaat gacacgatgg
      301 atccgatcat tgttccagat tgccagattc tcggaaaggt cattggtatt ttccgttttc
      361 tttcctgatg aagttcggta ttcctggaat aaaatgcaga ttcctattat aatgtacaag
      421 aaagaatcct gcttttcagg attctccgcc tgcggcgggt tgctctagcg acatcgcgcg
      481 agtgcacatc cctgcggagc ttttctatgt aacaggaacg aagtttccta ttatatagaa
      541 aaaagtatgc cccctttcct gtcagccaga agggaggcat actcttttta cgacactctt
      601 aagttcaaaa cacgcatcta tgacctttcc gtctctccgg aatcttacgg atccgccgca
      661 tctatgacct ttccatttct ccggaatctt acagatcagc cacatctacg atctttccgt
      721 ctctccagat acgctccaga tcatagaata aacgatcttc cttggaaaaa atatggacaa
      781 tgatgtcacc gtaatccatg agaatccagg tagaactaga atttccctcg atctgtttgg
      841 tcttaagtcc cgctttgtat aatgcctctt cgcaggcatc acgcattgcc tgaatctgat
      901 tctgattcgt tccgtccgca atcacaaaat aatctgcaac cggtgaaatc tcacggatat
      961 caatgatctt aacatcctcc gccttgcggt cttccaaagc cgctactgct attttacaat
     1021 attcctgtga tgtcattgca tgtttccttt ctatctgttc tttgttcgtt ttaacaggta
     1081 atccgtcaaa ccggatctgt ctgttccttc tggtactgcc ggtagaattc ataggcaagc
     1141 tgtgtggagg gatctaacgg tccgccgacc ctggagcggt atttcaatgt gtcgcagaga
     1201 atctccgcca tacaggcatc cagatcttca aatgccagtt tccgcaccct cggcagatct
     1261 gcggccttgt tccttcccgg ctcgatataa tccgcaatat agatgatctt atcgagcgta
     1321 ctcatatccg gcgcgccggt tgtgtgcacc ttgatggcgt gcaggatctc aggatctttt
     1381 acttcataga ccgtctctgc cagataagct cccagcttgg catgcagcag aaacgggctc
     1441 tcatattcta cggcattgat gggaatgtca tgttcctcac agatctgaag cttctcatcg
     1501 tccggaatac atttggcaca atcgtggagc agacctgcga gcatcgcctg ctcgatcgat
     1561 gtctcataag ccatcgccat acatccggcg gtatacatga cacctttcgt gtgctcatac
     1621 cgtcccttat ccaactcttt ttttaatcgt ttacgaatct catgctgttc catttttatt
     1681 ctcccaaatt cgatacgccg tacagatgat agcgtttgat cagctcccac acctcgcccg
     1741 ggagcatggc gggacattca cctgtcatgc gaatctgtgc gcggattgcc gtcgaggaga
     1801 catcggtccg tccgcccgca agcggataga tctcagccgg aaacaacgcc ttgatgcacc
     1861 gtatttttcc ctcaatctgc ggcagttcta aggtatccct gaccgctgca agcacggtgg
     1921 cattctgaca gatgatctcc ggatgcctcc acttgtcaaa gtagtccagg gaatcggctc
     1981 ccatgataaa gaagaattga tcctccggga actgttcctt tagatgctgc agcgtcttat
     2041 acgtataact tgtcccggaa gactctattt ctatacgtga aagcttaaat gacgcatcat
     2101 ttttgatggc cgcctgcgtc atttccgcgc gcacctgcgc gggtgtcatc tccgcctcct
     2161 ccttattcgg agagtgccct gccggtatga accacacctc gtccaacgcg aactcccggt
     2221 aggcgctcct tgcaatattc agatgtccct gatgaatcgg atcaaacgag ccgcctaaaa
     2281 ttccgatctt tctcatgccg gaagttcaaa cttgctgttt ttcttcgcct ggcggaataa
     2341 gatgatcttc ttgccgatga cttttacaac agcagaatgt gtgcgctctg cgatgacctg
     2401 tgcaatctcc cgcggatcat ccgcacaatt ctttaatacg gaaatcttaa tcagttcccg
     2461 tttctctaat gcttcatcca gcgcctggat aatctccggt gtcaggcttg ccttgccgat
     2521 ctgaaagatt ggatctaagg tgcttgcaca tccgccgaga taggcgcgct gcttacttgt
     2581 caattccata tcctgcttcc tcctctttac ttataataat caaattcaaa accgtacatg
     2641 cgcacggtat cgccgtcctc aattccctgt gcctccaggt cttttaaaat gccctgctcc
     2701 ttcatgaact tctggaagaa aagaaatccc ttctcggaat ccatattcgt atagccaagc
     2761 atcttctcga tcttcggtcc ttccacaacg aatgcaccgt cgtcagccac cgtaatcgtg
     2821 tatggctcat ccttaaagaa acggagtgcc ggatcgaatt caggctcata gacaaccggc
     2881 tccttgctgc actggtcgag aagctcgctc acgcagtaca acagttcctt taatcccttg
     2941 ccgcttacgg cagaaatcgg gaacacacgg attccgtctt tttcaaaggt gtcttttaac
     3001 ttctggataa tctcattctc gtccccgtag accgcatcca ttttatttgc cgcgatgacc
     3061 tgcggctttt ttaacagtgc cggatcgtaa gcttccagct ccttgttgat cgcgataata
     3121 tctgccacag gatcacgtcc ctcggtgccg gcagcatcca ccatgtggat cattaccttc
     3181 gtgcgctcga tatggcgcag aaattcaagt ccaagtccaa caccctcgga agcgccctca
     3241 atcagtccag gaatgtcagc aatgacgaaa cccttcgcgc cgtctaaatc caccacgcca
     3301 agattgggct gcagcgttgt aaaatgatag ttggcaattt tcggctgtgc gttggtcacg
     3361 cgcgataaca gcgtggattt cccgacattc gggaagccga ccagaccgac gtccgcgatt
     3421 acttttaatt ccagcttgac ttcaatctcg attgaatctc cgcccggctg cgcatatttt
     3481 ggtgcctgca tcgtcgatgt cgcgtaatgc tggttgccaa gtccgcctct gccgccccgc
     3541 aaaatgacct gtctgcggtt ctctccggac atatcggcga tgaccttgcc ggactctgca
     3601 tccttgatca cggttccctc cggcaccttt aagatgagat cctcaccgtt tttgccgtgg
     3661 cagttgcgct tgccgccctc ttctccgggc tgtgccgcaa actttctgcg atgacggtaa
     3721 tccgtcagag tgtttaaacc ttcatccacc acaaagatca cgtcgccgcc cttgccgccg
     3781 tcgccgccgt caggaccgcc atccggcaca tatttttcac ggcggaagct cacatgcccg
     3841 tcgccgcctt tccctgattt tatgataatt gttgctctgt ctgcaaacat tatattctac
     3901 ctcaattcaa cttcaggcag cagatgctgc cacctaccga acggttacaa atccagatac
     3961 aagaagaccc ggctatagac gcctgccatc aggtaagcgc tgcatagtcg ggtctacact
     4021 ctgcatctta ttactcagct actggataaa cagaagcctg cttcttgtct tttccttttc
     4081 tctcgaatct taagatacca tctgtcagtg cgaataatgt atcatcaccg ccgatgccta
     4141 cattaacgcc cggatgaatc tttgttccgc gctgtctgta taaaatgttg ccagccttca
     4201 cgaactgtcc gtctgctctc ttagcgccta atctcttaga ctcagagtca cgtccgttct
     4261 ttgtag
//
LOCUS       DBMDQJ010000133         4256 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7796_length_4256_cov_1.506619, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000133 DBMDQJ010000000
VERSION     DBMDQJ010000133.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7796_length_4256_cov_1.506619"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(158..721)
                     /locus_tag="ACZO9R_03840"
     CDS             complement(158..721)
                     /locus_tag="ACZO9R_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HIR0912235.1"
                     /translation="MKQQFYDAIVDGPIIAAVKDETGVEVCIQNDIRVVFILYGELIT
                     IPDIVQRLKDAGKFVVVHLDLIGGLAVREEAVRFIRYGTAADGIISTKPEMIRYAKEL
                     DLCTVFRIFAIDSKAIGGLEHHGMEFADLIEVLPGIMPKIIKHIAGQAAVPVIAGGLI
                     SEKEDVMRALDAGAIAISTTNQAVWKM"
     gene            complement(1058..1399)
                     /locus_tag="ACZO9R_03845"
                     /pseudo
     CDS             complement(1058..1399)
                     /locus_tag="ACZO9R_03845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.6"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(1423..2325)
                     /locus_tag="ACZO9R_03850"
     CDS             complement(1423..2325)
                     /locus_tag="ACZO9R_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SseB family protein"
                     /protein_id="HIR0912236.1"
                     /translation="MDEKMTMEITNDRLEEAIKQYAAERTKENLTTVLNLLRPTKLYV
                     PAMLKAPDQPTPCFLKNNKGEQFFVAYTSKEQMPEEPKSQALLFMPFPACNNIVVKPE
                     LELTGLVINPFSDNLVLKTELVRKLHEADQKAAQMRQVKMTPQQFNVFVKKQVEFGVL
                     PKRLYAEGETFVRKLCEEREAFVNQIFAETYKEPKLYPYTEADYSVMALDIAEDLTLI
                     RVDLPEKALAAPLCYRIYITFNPQTKKAGYYTIEMTAEKEVRALGQILPDGRHESLGE
                     APVEGAELQKIMDLARYDGTEMTS"
     gene            complement(2404..4191)
                     /locus_tag="ACZO9R_03855"
     CDS             complement(2404..4191)
                     /locus_tag="ACZO9R_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912237.1"
                     /translation="MGMKNVSVRWKMIILALITLIGTLAIGVLAVNRMLVMEEGSESV
                     LRTSIEDDYDENIKNQVDNAISMLDAVYAGYENGDYSYEEAETRGADLLRELRYGDGG
                     YFWADTYDGDNVVLLGSETEGTNRMETKDAEGYQMVKEIIRVGQEPDGGYTDYVFPKE
                     GETESSPKRSYSRAFEPFGWVVGTGNYIDYIDETVAKETQAMKENVKSALLAIIGMGS
                     FLVVIVMAVCLYMAFSLSKSFQVALTYIGYITKGDFSQPLPVQLENRRDDFGILGERL
                     ENMKCQIRDLIREVKNESYVIGEVVDTVKTNVITLNGNIEDVSATTEELAASMEETAA
                     SSDTIKSMSLEIEEAAKNIANRSQDGAQQAADIHERASKAQNDTREQREHASRIHNEI
                     RESLTKALEAAKVVQQIEVLSSAIMEITNQTNLLALNASIEAARAGEAGKGFAVVATE
                     IGGLADQSKQTVTQIQKVTEEVTTSVAQLSSDAEQLLAFVGNDVVASYDMFDAVADAY
                     NQDAGKIDALISDFSATSEELLASIDGVLDAMEGIATATNEGAKGTTDIAQKTVEVKS
                     EADTVTDEVGRCDQTAQRLTQDISVFVVD"
BASE COUNT          957 a         1292 c          794 g         1213 t
ORIGIN      
        1 aaaactctgg cgactcatcc ccgttgcctc catgatgtcc ccgatcgtaa ttttggatat
       61 ctccttgcgc ttcatcagct gcctgacaca atccgcaatt tttttcttag ttgaataaca
      121 catacccgta cccccttctc ctgatacact gtatccgtta cattttccac actgcctggt
      181 ttgtcgttga aatcgcaatc gctcccgcat ccagtgcccg catgacgtcc tccttctccg
      241 agatcagacc tcctgcgatc accggtactg ctgcctgccc tgctatatgc tttatgattt
      301 taggcataat gcccggcagc acctcgatta gatctgcaaa ctccataccg tggtgttcca
      361 gtcctccaat cgccttggaa tcaatcgcaa atatgcgaaa taccgtacag agatccagct
      421 ccttcgcata acggatcatt tccggctttg tggaaataat accgtccgcc gccgtcccat
      481 accgtataaa acgcaccgct tcctccctga ccgcaagccc cccgatcaga tccagatgta
      541 caacgacaaa ctttccggca tcttttaacc gctgcacgat atccgggatg gtaatcaact
      601 ctccatacaa aatgaatacc acgcggatat cattctgtat acagacctcc acccccgtct
      661 cgtccttcac cgccgcgata atcggtccgt ccacaatcgc gtcataaaat tgctgcttca
      721 tcttttgtct gcactccccc cattctcccc tgccgcatca tggcattcct ggcgcatcat
      781 ttccataatg atatttccca tagcggaagt caaaaaatcc cgcaagctgc acattcttta
      841 tttcttcata gggcgtaatt tcctatgaaa aaaagcacac cgacagagta acatttctgt
      901 tattcccggc ctgctctttc actcttggct cacactatag aattgtatgc aaaataccgt
      961 gtaatttctt tctgacatta ttataactat gtcaaaaagg aaacgcaacc gaatttcaga
     1021 ttttgcattt gacaatttca gcgttttgtc aaaaaactca catcggattc tcaaggtatt
     1081 ccgccagcac ctgatataac cgctgcagct ctaccggctt tgcaagatag tcgttcattc
     1141 cggcttcctt acaggcatcc atatcctcgc gtgcatcttt tgcactcatc gcaataatcg
     1201 gaatcatcta cgcatccttg tgcgacaccc gtcggatctg ccttgccgcc tcaaagccgt
     1261 ccatcttcgg catcacaata tccatcagga tcgcatcgaa ataatgcgct tccttctcca
     1321 taaaaagacg cacctcggtt cccgccggac atgcgtctct tattgtatct ttatgtatgc
     1381 cttcgtttcg atatgacata tatgccttcg cttcggtatg ccttagcttg tcatctctgt
     1441 gccgtcataa cgtgccagat ccatgatctt ctgaagctcc gctccctcaa ccggcgcctc
     1501 gcccagactc tcatgcctgc catccggcag gatctgtccc aatgcgcgca cttccttctc
     1561 cgccgtcatc tcaatcgtgt agtaacccgc tttcttcgtc tgcggattaa acgtaatata
     1621 gatacgatag caaagcgggg ctgccagtgc tttctccggc agatccacgc ggatcagtgt
     1681 cagatcctcc gcaatgtcaa gcgccataac cgaatagtct gcctctgtgt acggatagag
     1741 cttcggctcc ttatacgtct cggcaaaaat ctggttcacg aacgcctcgc gctcctcaca
     1801 gagcttccgg acaaacgtct ctccctctgc atacaggcgc ttcggcagaa ctccaaactc
     1861 cacctgcttt ttcacaaaca cgttaaactg ctgcggtgtc atcttcacct gccgcatctg
     1921 tgccgccttc tgatctgcct catgcagctt ccgtacaagt tcggtcttta atacaaggtt
     1981 atccgagaac ggattgatga ccagcccggt cagctccagt tccggcttca cgacgatgtt
     2041 gttgcacgcc gggaacggca tgaaaagaag tgcctggctc ttcggctcct ccggcatctg
     2101 ctccttggaa gtatatgcca caaaaaactg ctcgcccttg ttgttcttga ggaaacacgg
     2161 ggtcggctga tccggcgcct taagcattgc cggcacatac agcttggtcg gacgaagcag
     2221 gtttaacacc gtggtaaggt tctccttggt gcgctccgcc gcatactgct taatcgcttc
     2281 ctctaatcta tcgttggtga tctccattgt cattttctcg tccatcttat aagtatcctt
     2341 tcctatgctc cgatgtcgct aaaatgaatt atatcatatc tttcccataa aatgaactgt
     2401 tttctaatcc acaacaaaga ctgagatatc ctgtgtcaga cgctgtgccg tctgatcgca
     2461 tctgccaact tcatctgtga ccgtatccgc ctcggatttg acttccacgg tcttttgtgc
     2521 aatatctgtc gttccttttg caccttcgtt cgttgccgtt gcaattccct ccatcgcatc
     2581 cagcactcca tcgattgatg ccagcagttc ctccgatgtc gcgctgaaat cgctgatcag
     2641 tgcatcaatc tttcctgcat cctggttgta tgcatcagcc accgcatcga acatgtcata
     2701 gcttgccacg acatcatttc ccacaaatgc aagcagctgc tccgcatcgg aagaaagctg
     2761 tgcgacagaa gtcgtaactt cctccgtcac cttctgtatc tgggtaacag tctgcttgga
     2821 ctgatctgca agtccgccaa tttctgttgc cacaacggca aatcctttgc ctgcctcccc
     2881 ggcgcgtgct gcttcaatcg aagcatttaa cgcaagcagg tttgtctggt ttgtaatctc
     2941 catgatcgcc gatgataaca cttcgatctg ctgaactacc tttgcagcct caagtgcctt
     3001 tgtaaggctc tcccgtatct cattgtggat ccggctcgca tgctcacgct gttctctggt
     3061 atcgttctgt gcctttgatg cccgctcgtg tatatctgct gcctgctgtg ccccatcctg
     3121 cgaacgattt gcaatatttt ttgctgcctc ttcaatctca agagacatgc tcttaatcgt
     3181 atccgaggat gccgcggtct cctccatgct tgcagcaagt tcctctgtcg ttgcggaaac
     3241 gtcctcgata ttgccgttca gcgtaatcac attcgtcttg accgtatcga cgacctcccc
     3301 gattacataa ctctcatttt tcacttctct gataagatct ctgatctggc acttcatatt
     3361 ctcaaggcgc tcgccgagaa taccaaagtc gtccctgcgg ttctcaagct gtaccggcag
     3421 cggctgcgaa aaatcaccct ttgtaatata accgatgtac gtgagtgcaa cctgaaatga
     3481 tttgctcagt gaaaacgcca tataaagaca gaccgccata acaatcacaa ccagaaaact
     3541 tcccattccg ataatcgcaa gcagtgcact ttttacattc tccttcattg cctgtgtctc
     3601 tttggcaaca gtctcatcaa tataatcgat ataattgccc gttccaacaa cccatccaaa
     3661 cggctcaaat gccctgctgt aggagcgctt cggagaggac tccgtctccc cttccttcgg
     3721 gaatacataa tctgtataac cgccgtccgg ctcctgtccg acacgtataa tctcttttac
     3781 catctggtaa ccttccgcat cctttgtctc catacggttt gttccctcgg tctcgcttcc
     3841 gagcagaaca acattgtctc cgtcataggt atccgcccag aaataaccac cgtctccata
     3901 ccggagttcc cttaaaagat ccgctccgcg cgtttcggcc tcctcatagg aatagtcgcc
     3961 gttttcatag cccgcataca ccgcatccag catggaaatt gcattatcca cctgattctt
     4021 gatattctca tcataatcat cttcaatact tgtacggaga acgctctctg atccctcctc
     4081 cataaccagc atccggttca ctgccagaac tccgatcgcc agtgttccaa tcagcgtaat
     4141 cagcgcaaga ataatcatct tccaacgcac gctcacattt ttcataccca tccgactcct
     4201 tcctgatagt tatttctttt attatcctac tttttcattt ttttgtctat agatgt
//
LOCUS       DBMDQJ010000134         4253 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7803_length_4253_cov_1.570568, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000134 DBMDQJ010000000
VERSION     DBMDQJ010000134.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7803_length_4253_cov_1.570568"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /gene="rpoD"
                     /locus_tag="ACZO9R_03860"
     CDS             <1..631
                     /gene="rpoD"
                     /locus_tag="ACZO9R_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HIR0912238.1"
                     /translation="LIQEGNLGLIKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQA
                     RTIRIPVHMVETINKLIRVSRQLLQELGREPTPEEIAAEMNMPVERVREILKISQEPV
                     SLETPIGEEEDSHLGDFIQDDNVPVPADAAAFTLLKEQLEEVLGTLTEREQKVLTLRF
                     GLEDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSRKLKDYLE"
     gene            635..1357
                     /locus_tag="ACZO9R_03865"
     CDS             635..1357
                     /locus_tag="ACZO9R_03865"
                     /EC_number="2.1.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0160105 - tRNA
                     (adenine(22)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase"
                     /protein_id="HIR0912239.1"
                     /translation="MVKISERLRTAAGLIGEGERLADVGTDHGYVPIYLVERKHIPSA
                     IAMDIRTGPLERAREHIRMYGMEDYIQTRLSDGVAALKPGEADTILIAGMGGGLVKHI
                     LESGRVICEQAHGLVLQPQSELPKVRRFLMENGYVTEREEMVMEDGKYYPMMRVHFDA
                     SAVSHTDSFTEEQELEFRYGKQLLTEKHPVLLAYLRWEAQIQQDILTQLSGQPATEQI
                     AARIDDVKQILAQNARALAYYE"
     gene            1398..3080
                     /locus_tag="ACZO9R_03870"
     CDS             1398..3080
                     /locus_tag="ACZO9R_03870"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase family protein"
                     /protein_id="HIR0912240.1"
                     /translation="MGKDMVSLTIEGEVRQYPAGTSFLAISQEYQEKYPDDIVLVVYN
                     NRLRELNKTAQRDGTLSFVTTADKTGKKTYRRSVTLLMQKAVYNLWGSEHISVRVLYS
                     IGQGYYCELREKADGQERVIAVDEERAIALKKEMYRLVTEDIEIEKRSMNTDDAIALF
                     HDLGMEDKEKLFKYRRSSRVNIYVLDHYKDYFYGFMVPSTGYLRYYDIVTYEDGFVLL
                     FPNENTREVAEFAPSGKLFHTLKASREWGRMLEIGTIGALNDAIAEGRMQEIILTQEA
                     LFEERIGHLADTIVKSGGKKFIMIAGPSSSGKTTFSHRLSIQLAAKGLKPHPFPLDDY
                     YVNRDQCPRDENGELDLECLEALDVELFNHDMNELLAGKRVNLPVFNFKTGKREYKDR
                     YMQLGKEDVLVIEGIHGLNDRLSCSLPVESKFKIYISALTQLNIDEHNCLPTTDGRMI
                     RRIVRDARTRGTSAKETIAMWDSVRRGEERYIFPFQEGADVMFNSALIYELAVLKMYA
                     EPLLFAIDKDCPEYLEAKRLLKFLDYFLPMPSDGISQNSIIREFIGGSCFNV"
     gene            3135..3506
                     /gene="rnpB"
                     /locus_tag="ACZO9R_03875"
     ncRNA           3135..3506
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACZO9R_03875"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            3667..>4253
                     /locus_tag="ACZO9R_03880"
     CDS             3667..>4253
                     /locus_tag="ACZO9R_03880"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR0912241.1"
                     /translation="MNGIKLFNDLMEYADGKGASDVHLVPGRTAMLRIDGELTAAGQE
                     DAAEWQDADLTEVTEQILSGDQLEEYRTKGALVCSYSFSKALRLRVSVYRQQGRDAMS
                     VRLHPAGVPMPEKLLLPEEITELVKNGRGLLLVTGGRGSGVTTTVASLAAQIAATQSK
                     VIVSVEDTTEYILPQGRGLVVQREIGAGNRYHGEMG"
BASE COUNT         1121 a          936 c         1291 g          905 t
ORIGIN      
        1 tctgattcag gagggaaatt taggactgat caaggcggtg gaaaagtttg attaccgcaa
       61 gggatataag ttctccacct acgcgacatg gtggatccgt caggcaatca cgagagccat
      121 tgcagatcag gcgagaacca tccgtattcc ggtgcatatg gtcgagacga tcaacaagct
      181 gatccgtgtc agcagacagt tgctgcagga gctgggacgc gaacctactc cggaggagat
      241 cgctgcggag atgaacatgc cggtggagcg tgtgcgtgag atcttaaaga tttcgcagga
      301 gccggtatcc ttagagactc cgatcggtga ggaggaggac agccatctcg gtgattttat
      361 ccaggacgac aacgttccgg taccggcgga tgcagcggca tttaccctgt taaaagaaca
      421 gttggaggaa gtgctcggaa cgctgacaga gcgtgagcag aaggtgctga cactgcgttt
      481 tggactggag gacggacgtg cgaggacact tgaggaagtc ggaaaagagt tcaatgtcac
      541 acgtgagcgt atccgtcaga tcgaggcgaa ggcgctgcgc aagctgcgtc atccgagcag
      601 aagccgcaaa ttaaaggatt atctggagta atctatggta aaaatatcag aacgtttaag
      661 aacggcggca ggactcatcg gagagggaga gcggctggcg gatgtgggaa ccgatcacgg
      721 gtatgtaccg atttatctgg tggaacgaaa gcacatcccc agtgcgattg cgatggatat
      781 ccggacggga ccgctggagc gtgccaggga acacatccgg atgtacggca tggaagacta
      841 catacagact cgtctgtcgg acggcgttgc ggcgctaaag ccgggtgagg cggacaccat
      901 cctgattgcg gggatgggcg gcggactggt caaacatatt ctggagagcg ggagagtcat
      961 ctgtgagcag gcacatgggc tggttttgca gccgcagtca gagcttccga aggtgcggcg
     1021 ttttctgatg gagaacggtt atgtcacaga gagagaagag atggtcatgg aggatggaaa
     1081 atattacccc atgatgcgcg ttcactttga tgcttccgca gtctctcata cagacagctt
     1141 tacagaagaa caggaactgg aattccgcta tggaaagcag cttcttacag aaaaacatcc
     1201 ggtgcttctg gcgtatctcc gctgggaagc gcagatccag caggatattt taacacagct
     1261 atccggtcag ccggcgacgg agcagattgc agcccggatt gacgacgtaa aacagatcct
     1321 cgcgcagaac gccagggcgc ttgcgtatta tgaatgacaa aaagccttga agaaaaggca
     1381 gagggggaga gattcgtatg gggaaagata tggtcagctt gacaattgaa ggggaagtaa
     1441 gacaatatcc ggcgggaacc agtttccttg caatttcgca ggaatatcag gaaaaatatc
     1501 cggatgacat cgtgcttgtg gtgtataata accggctgcg ggaattgaat aagacggcac
     1561 agcgggacgg aacgctttcg tttgtgacaa cagccgataa gacgggaaaa aagacatacc
     1621 gcagaagcgt cacgcttctg atgcagaagg cggtctacaa tctctgggga agcgaacata
     1681 tcagcgtccg tgtactctat tccatcggtc agggatatta ctgcgagctg cgtgaaaagg
     1741 cagacggaca ggagcgcgtg attgcggtgg acgaagagcg cgccatagcg ctgaaaaagg
     1801 agatgtaccg tctggtgacg gaggatatcg agattgagaa gcgcagcatg aacacagatg
     1861 atgcgattgc actgttccat gacctcggga tggaggataa ggaaaagctt tttaaatacc
     1921 gccgcagttc cagagttaat atctatgtgc ttgatcacta taaggactat ttttacggat
     1981 tcatggtgcc gtctacgggc tatctccgct actacgatat tgtgacgtat gaggatggat
     2041 ttgtacttct gttcccgaat gagaatacaa gagaagtggc agagtttgca ccttccggga
     2101 agctgttcca cacgctgaag gcttcgcgtg agtgggggcg catgctagag atcggcacga
     2161 ttggcgcgct taatgacgcg attgcggaag gacgcatgca ggaaattatt ctgacgcagg
     2221 aggcactgtt tgaggaacgg atcggtcatc tggcagatac gattgtaaag tcgggaggca
     2281 aaaagtttat catgattgcc ggaccgtctt cctccggaaa gaccaccttt tcacaccgcc
     2341 tttctatcca gcttgctgcg aagggattaa agccgcatcc gttccctctc gatgattatt
     2401 atgtgaacag ggatcagtgc ccgcgcgatg agaacgggga actcgatctg gaatgtctgg
     2461 aagcgctgga tgtggagctg tttaaccacg acatgaatga actgcttgcg ggcaagcgcg
     2521 tcaatctgcc ggtcttcaat tttaagaccg gcaagcggga atacaaggac cgctacatgc
     2581 aacttggaaa agaggacgta ctggtcatcg agggaatcca cggactcaac gacaggctgt
     2641 cctgctcgct cccggtggag agcaagttca aaatctacat cagtgcgctg acccagctca
     2701 acatcgacga gcacaactgc ctgccaacaa cggacggcag aatgatccgc cggattgtga
     2761 gagatgcgcg cacgagagga acttccgcaa aggagaccat tgccatgtgg gattcggtgc
     2821 gccgcggtga ggagcgctac attttcccgt tccaggaggg ggcggacgtg atgtttaact
     2881 cggcgctgat ctatgaactg gcagtgttaa agatgtatgc cgagccgctg ctgtttgcca
     2941 ttgacaagga ttgtccggaa tatctggagg caaagcgcct gctcaagttt ctggattatt
     3001 tccttccgat gccgagcgac ggtatcagcc agaattcgat tatccgcgaa ttcatcggcg
     3061 gttcctgctt caatgtatag gattgctttg acattttgcg gaaagcacat tataataagc
     3121 aatagttgcg gtgataagta gaacaaatga tcgcggccca gcattgctgg ggtgaggaaa
     3181 gtccgggctt cacagggcag gatgccggat aacgtccggt ggaggcgact ccaaggatag
     3241 tgcaacagaa atataccgcc ggttccggta atgccggaat acggtaaggg tggaagggca
     3301 gtgtaagaga ctaccgccgc gctggtaaca gagcgggcat atgtaaaccc catccgaagc
     3361 aagaccgaac aaaagcgttg acgcggcccg ccagctttag gtaggttgct tgaggtggct
     3421 ggcaacagtc atcctagata gatgatcatt ccgaccgtgg cttgccacgg tttagacata
     3481 acccggctta tcgttctgct tatcattgtc acatagaagt atctgagagt cgcgcagcgg
     3541 ctctttttat ttcataggga ataccgtgat acattaatgt gtgaaagtag aaccttccta
     3601 gaaataaaaa acgctctgcg gggatgcatt gcatttccct atgaagcaat aagaaggagt
     3661 ttttctatga acggcataaa actttttaat gatcttatgg aatatgcgga tggcaaaggt
     3721 gcatcggatg tacaccttgt cccgggaaga acggcgatgc ttcggattga cggagaactg
     3781 actgccgcag ggcaggagga cgccgcagaa tggcaggatg cggatcttac agaggtgacg
     3841 gagcagatct tgtccgggga tcagctggaa gagtaccgca cgaaaggcgc gctggtatgc
     3901 agctattcat tttccaaagc gctgcgtctg cgcgtgtccg tatacagaca gcaggggaga
     3961 gatgccatgt ccgtaagact gcatccggcc ggggttccga tgccggagaa acttctgtta
     4021 ccggaggaga ttacggagct tgtgaaaaac gggcgcgggc tgcttcttgt cacgggaggc
     4081 agaggaagcg gagtgacgac gacggtggca tcacttgccg cacagattgc agcaacacag
     4141 tcaaaggtca ttgtctctgt ggaggatacg acagaataca tccttccgca gggaagggga
     4201 ctggttgtgc agcgggagat cggagcggga aaccgatatc acggagaaat ggg
//
LOCUS       DBMDQJ010000135         4221 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7872_length_4221_cov_1.629126, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000135 DBMDQJ010000000
VERSION     DBMDQJ010000135.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7872_length_4221_cov_1.629126"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(267..737)
                     /locus_tag="ACZO9R_03885"
     CDS             complement(267..737)
                     /locus_tag="ACZO9R_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912242.1"
                     /translation="MDYNQEQFFHAQFDVPKMPFYMSYPMQNLYLTEMEYEKDMDRMK
                     ELYPKEVGRIVECVEDECDRMEYEGSLMFDEYPDRLMIEMVVDRIQKKVTGEDGGLEA
                     EQFHGGMQPPPGHRPPPPPPPHGQNDHLRELIGVILNNEMYRRRCRHRRCRRWW"
     gene            complement(793..1002)
                     /locus_tag="ACZO9R_03890"
     CDS             complement(793..1002)
                     /locus_tag="ACZO9R_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912243.1"
                     /translation="MKIMNARNKFVENHPKFAAFLNAVFSAGITEGTVIEITVTKPGE
                     EPITTNMKVLQSDLDLLDELKNLAQ"
     gene            1287..1553
                     /locus_tag="ACZO9R_03895"
     CDS             1287..1553
                     /locus_tag="ACZO9R_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912244.1"
                     /translation="MQLPCTSGKQGYREFADKLFLNTMLHNRTRHNLYAAFSREEQNI
                     INTFLCVTSVNIYRQWVADKKRVPLERLIALSGTLLNNGVVSLF"
     gene            complement(1545..3671)
                     /locus_tag="ACZO9R_03900"
     CDS             complement(1545..3671)
                     /locus_tag="ACZO9R_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR0912245.1"
                     /translation="MKIKFAQTIRNYDRKNLGKDLVAGLIIMAVSIPISMGYAQIAGL
                     PAVYGLYGSVFPILLFALFSTSPQFIFGVDAAPAALIGAALLDLHIESGSKEALAAVP
                     VLTAFVALWLFAFYVMRAGKLVNYISAPVMGGFITGICTTIILMQVPKLLGGTAGTGE
                     FFELAEHIAETVGEINLPSLALGVAALVILLIAKKLIPKFPMAVLLMAAGALMTKYLP
                     MRAWGIKTLDAVEPGLPVWSLPDFTAVPVREAVTISLSVAVVIMAETLLAENNFAQKN
                     GYRINDNQEILAFAMGNLAAALTGCCPINGSVSRTAMGEQYQAKTQLTGIVAGLSMIV
                     LLLGGTGFIGYLPVPVLTAIVISALLGATEFELAVRLWKVSRTECLIFAGAFFGVLML
                     GTINGVLIGIILSFSEMIIRTAKPARCFLGIQPGHRHFRDLKEGSQIHAVEGVLIYRF
                     SSNLFFANIQVLKQDIEDHVTDQTRAVILDASGIGSMDITAADGLGMLYQSLKEKGIR
                     FYMTEHIADLNEQLRKLGLGYMIEEGSVRRTIHIALKDMGIKRPYPLEGGVDNDDRSA
                     SRKRADNRVQEFVWAFGAETEEQIEKQILLQIEQLKESGDVENLLHGRWSHMEAMDED
                     EWLEHLEEHLKEIVNISGKDEKTLARRLEEHRREVHDRIATEHPELAERFKERRHVLD
                     EHLKQRRPEVYELVVSLREKEETDLK"
     gene            complement(3750..>4221)
                     /locus_tag="ACZO9R_03905"
     CDS             complement(3750..>4221)
                     /locus_tag="ACZO9R_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912246.1"
                     /translation="IIATIEEIVDQTNLLSLNASIEAARAGEAGKGFAVVADEIGKLA
                     QESSKAANMTRELIGVSMEEINKGNQIADHVMDSLKTAVEAVDNVNGMIQKTAGNAAD
                     QAQSMEQIRVGIEEISQGVQDNSAIAEESSATSEELASQATLLNELVQHFELQP"
BASE COUNT          942 a         1279 c          921 g         1079 t
ORIGIN      
        1 ttcataaatc ttccaaatat aagcactgct ccgatgaaca ctgccgccaa gactgctgcc
       61 tttatggttc ccttccgcaa cggtcattcc tcctatataa cgcaaaataa ctaccgcgca
      121 caaaaacgcg atagttatat taacacaaaa taactatcgc acaaaaacgc gatagttata
      181 ttgtatgcat tttaaaataa atccgcaatg actttctctg atttttaaga attgttttcg
      241 atcttatccg gggcttcgca cccccgttac caccagcgtc tgcagcggcg gtgacggcag
      301 cgtctccggt acatttcgtt gtttaaaatc acaccgatca gctcccgcag atggtcattc
      361 tgtccatgcg gtggcggcgg tggcggcggt ctgtgtccgg gcggcggctg catgcctccg
      421 tgaaactgct ctgcctccag cccgccgtct tctcctgtga ctttcttctg gatgcggtct
      481 accaccatct caatcatcag gcggtccgga tactcatcga acatcaggct gccctcgtat
      541 tccattctgt cacattcgtc ttccacacac tccacaatcc tgccaacctc tttgggatac
      601 agctccttca tgcggtccat atccttctca tactccatct cggtcagata cagattctgc
      661 atggggtagc tcatataaaa aggcatcttg ggcacatcaa actgcgcgtg gaaaaactgc
      721 tcctgattat agtccactct atactcccta tagataactt cgctatatca tatgcagtgc
      781 gcgcgcaccg ggttactgcg ccagattctt taactcgtcc aataaatcca gatccgactg
      841 gagcaccttc atattggtcg tgatcggctc ctcgcccggc ttggtgacgg tgatttcaat
      901 taccgttccc tccgtaatgc ccgcagaaaa taccgcattt aaaaacgccg caaacttcgg
      961 atggttctca acgaacttgt ttctcgcatt cataatcttc ataagtgaag ccggattcat
     1021 cataatatca tactcccttt cttctgttgt gtcgttctgt ctcccagtat agcacaactg
     1081 ccgcggcact tgccagcatt cttcatccat ttctgatcat ggcactcgtt gtatttctga
     1141 ttgtaaaggc attaaaccgt gcaaccgcaa agttcgttcc cgcggaggag aaaaagaaga
     1201 agcaccaacg agcaaactct gtccgatgtg taagaccgag attgccattg atgcctgccg
     1261 ctgcccgcat tgcacttcca ttctcgatgc agttgccatg cacgagcggg aagcagggct
     1321 accgcgaatt cgccgataaa ttattcttaa acaccatgct tcacaaccgc acgagacata
     1381 atctctacgc cgcattttcc agggaggagc agaatatcat caacacgttt ttatgcgtca
     1441 cctccgtcaa tatttaccgg cagtgggtgg cagacaaaaa aagagtgccg cttgagaggc
     1501 tgattgccct ctccggcacc ctgttaaata acggtgttgt ttctttattt taaatctgtc
     1561 tcttcctttt cgcgcaggct caccacaagc tcatagacct ccggtcttct ctgctttaag
     1621 tgctcatcca gcacatgtct gcgctccttg aagcgctccg caagctccgg atgttctgtt
     1681 gcaatgcggt catgcacctc cctgcggtgt tcctccagac gtcttgcgag tgtcttctca
     1741 tcctttccgg agatgttcac aatctccttt aagtgttctt ccagatgttc cagccactcg
     1801 tcttcatcca tcgcttccat gtgcgaccat ctgccgtgca gcagattttc cacatccccg
     1861 ctctccttca gctgttcaat ctgcaaaaga atctgttttt caatctgctc ctccgtctcc
     1921 gcgccgaacg cccagacaaa ttcctgcaca cggttgtccg cgcgctttct cgaggcactg
     1981 cgatcatcat tgtccacacc gccctccaac ggatacgggc gcttgattcc catatctttt
     2041 aacgcgatat gaatcgttct gcgcacgctt ccctcttcga tcatgtagcc aagccccagt
     2101 ttccggagct gttcgttcaa atcggcgatg tgctctgtca tgtaaaagcg gatgcctttt
     2161 tctttcagcg actgatataa cattcccagt ccgtccgccg cggtgatatc catactgccg
     2221 atgccgctcg catccagaat gaccgctctc gtctgatcgg tcacgtgatc ttcaatatcc
     2281 tgttttaata cctgtatgtt ggcaaaaaac aggttgctgc tgaaccggta gatgagtact
     2341 ccctcaaccg cgtggatctg gctgccctct ttcaggtcgc ggaaatgccg gtgtcccggc
     2401 tgaattccca gaaaacagcg cgccggcttt gccgtgcgga taatcatctc ggaaaacgag
     2461 aggatgatgc cgatcagtac gccgttgatc gtcccgagca tcagcactcc gaagaatgcg
     2521 ccggcaaaga tcagacattc cgtgcggctg actttccaca gccggaccgc cagctcaaac
     2581 tccgtcgcgc caagcagagc tgagatcacg attgccgtca gcaccggcac cggaagatag
     2641 ccgataaacc cggtgccgcc aagcaggagc acgatcattg aaagtcccgc cacaatcccg
     2701 gtcagctgtg tctttgcctg atactgctct cccatcgccg tccgggagac cgagccgttg
     2761 atcgggcagc atccggtcag cgccgcggca agattgccca tcgcaaatgc caggatctcc
     2821 tgattatcgt tgatccggta tccgtttttc tgcgcaaaat tattttctgc gagcagcgtc
     2881 tccgccatga tgacgaccgc caccgacagg ctgatcgtaa ccgcctccct taccggcacc
     2941 gccgtaaaat ccggcaggct ccagaccgga agtcccggct ccacggcatc cagcgtcttg
     3001 atgccccagg cgcgcatggg cagatacttt gtcatcagag cacccgctgc catcaaaagc
     3061 accgccatcg ggaatttcgg gatgagcttt tttgcaatca gcagtatcac aagcgcagcc
     3121 acgccaagtg ccagggacgg cagattgatc tctcccaccg tctccgcgat atgctccgca
     3181 agctcaaaga attctcccgt ccctgcggtg ccgcccagaa gcttcggcac ctgcatcaga
     3241 ataatagtcg tgcagatgcc ggtgataaag ccgcccatca ccggcgccga gatatagttg
     3301 accaactttc ccgcccgcat cacgtaaaac gcgaaaagcc acaacgccac aaacgcggtc
     3361 agcaccggca ctgccgccag cgcctccttc gatccactct cgatatgcag atctaaaagc
     3421 gccgctccga tcagcgcggc aggcgctgca tccacgccaa aaataaactg cggtgatgtc
     3481 gagaacagcg cgaacagaag aatcgggaac accgatccgt acagtccgta tactgccggc
     3541 agtcccgcga tctgcgcgta tcccatggag atcggaattg agactgccat gatgatgagt
     3601 ccggcgacca gatcttttcc aagattcttc cggtcataat ttctgattgt ctgtgcaaac
     3661 ttgatcttca tctgtcacac ttcctttgct gcttttgtgt tattttacca ctttaggaaa
     3721 ataatgtcag caaaagggat tcggtgtttt tatggctgca gttcaaaatg ctgcaccagt
     3781 tcattaagca gtgtcgcctg ggatgcaagt tcttcggagg ttgcggagct ctcctcggca
     3841 atcgccgaat tgtcctgtac tccctgcgag atctcctcga ttcccacgcg gatctgctcc
     3901 atgctctgtg cctgatccgc cgcatttccc gccgttttct ggatcattcc attaacgttg
     3961 tccactgctt ccactgccgt ctttaacgaa tccatcacat gatctgcaat ctgattgcct
     4021 ttattgatct cttccatcga gacaccaatc agttcccgtg tcatatttgc cgcttttgaa
     4081 ctttcctgcg caagctttcc gatctcatcc gccacaaccg caaagccttt tccggcctcg
     4141 cccgcacgcg ccgcctctat ggatgcattt aaagacagca ggttggtctg gtctacaatt
     4201 tcctcaatgg tcgcaataat t
//
LOCUS       DBMDQJ010000136         4177 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7952_length_4177_cov_1.534347, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000136 DBMDQJ010000000
VERSION     DBMDQJ010000136.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7952_length_4177_cov_1.534347"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            176..1072
                     /locus_tag="ACZO9R_03910"
     CDS             176..1072
                     /locus_tag="ACZO9R_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0912247.1"
                     /translation="MDINYELYKVFYYVATSLSFSEASKQLYISQSAVSQSIKTLEKK
                     LEQPLFIRSTKKVQLTPAGKILLKHIEPAMNLIRRGESQLLDSGSLGLGQLHIGASDT
                     ICRYFLVPYLKQFHKKFPNVPIKVTNSTSLSCVELLEQGKVDLIVTNFPNSNLNQSYI
                     RKTVCSFHDVFIANPAYFNLKQQEIALEDLQQYPILMLDRKSTTSEFLHNLFLQHQLE
                     LMPQIELSSNDLLIDLARIGLGIAFIPDYCLNRESKELFIVKIKEKLPARQMVTAVNP
                     TLPIAQSTEEFLKLLPSLPELG"
     gene            complement(1037..3688)
                     /locus_tag="ACZO9R_03915"
     CDS             complement(1037..3688)
                     /locus_tag="ACZO9R_03915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR0912248.1"
                     /translation="MDEQQEKDEKKIIKISVRNLVEFILREGDIDNRHGRTASPEAMW
                     EGSRIHKRIQKSKGANYHAEVPLKIELSEGDYTLAIEGRADGIEITCDGERQPDFSDN
                     FNHLAVEEGMHVTIDEIKGIYMNLDALDEPVGVHRAQAMCYAYIYALQHDLAEISVQL
                     TYCNLDTIELTKTAFLGNLKYFRETFSFAELAEWFTRLTDEYKKWADFQFAWQNLRQA
                     SIKKLEFPYAYRKGQKELASDVYRTIARRKNLFIQAPTGVGKTISTVFPAVKAVGEGL
                     GDKIFYLTAKTITGTVAKEAFELLRTRGYQAKIIWLTAKEKLCLCEEMDCNPVHCPYA
                     KGHYDRVNDAVYNLLQKEDVFTREVILEQAREYRVCPFEMSLDTATWADDIIGDYNYV
                     FDPNVYLKRFFAEGTRGDYIFLVDEAHNLVERSREMYSAAIYKEDFLAVKKLLKPYQE
                     KHDRRAERVIRHLEKCNRILLGYKRECENYVVYENIGTLIFALTRLAADLDEYLQKSP
                     DFPERREVNEFYLNLRNFMNIYELVDEHYVVYSEHEEDGRFKLKFYCVDPSLNLQERI
                     DKGNATIFFSATLLPIQYYKSLLSTRRDNYAVYAQTAFSEEQRLLLFGNDVSSKYTRR
                     GRAEYERIALYIEKTARAKQGNYMVFFPSYRMMQEVYDVFLEGGETDEMRPQEYFPEG
                     AENAEIVEHPEEAEIAEHPEEAEIAEHPNDAENPRDAEPCLWCMMQQTGMREAEREAF
                     LQAFSGEASKRRGGSLVAFCVLGGIFGEGIDLKKEQLIGAVIVGTGLPQISNEREILM
                     RYYDERAGAGFDYAYRFPGMNKVLQAAGRVIRTTEDVGVIELLDERFLKSDYRGLFPR
                     EWEQRRICNVNEVEQMLAEFWERRQEF"
     gene            complement(3799..4026)
                     /locus_tag="ACZO9R_03920"
     CDS             complement(3799..4026)
                     /locus_tag="ACZO9R_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR0912249.1"
                     /translation="MIERKIRLSDTEEVKEFVRAAGKCDFDIDVCYNRAVIDAKSLLG
                     MLYLGLSKDLTVKYGGQNTGFENTVQKYAVC"
BASE COUNT          979 a         1243 c          853 g         1102 t
ORIGIN      
        1 tatgaagtat agcatatgcg aacaaataag cataattaat atatttaatc tcttctatta
       61 ttatggttaa tatatcacat aagatttatt tgacagtttt ataagcaggt cttataatta
      121 tagttaacac gctacagaac ggttcggagc agcgctccga cagaaacgag gaaccatgga
      181 tatcaattac gaattgtata aagttttcta ttatgttgca acctccctca gcttttccga
      241 ggcgagcaag cagctctata tctcccagtc cgccgtcagc cagtcaatca agacgctgga
      301 gaaaaagctg gagcagccgc tttttatccg gagtaccaaa aaagtacagc tgactccggc
      361 aggaaagatt ctgctcaagc atattgaacc tgccatgaat ttaatccggc gcggtgagag
      421 ccagctgtta gattccggca gcctcggtct ggggcagctc cacatcggcg cgagcgacac
      481 catctgccgc tattttctcg tgccgtattt aaagcagttc cacaagaagt tcccgaatgt
      541 cccgatcaag gtgacaaact caacctccct cagctgtgtg gagctgttgg aacagggaaa
      601 agtcgatctg attgtgacca attttcccaa ctctaattta aaccagtcct acatccgcaa
      661 gactgtgtgc agctttcacg acgtctttat cgccaaccct gcctatttta atctgaagca
      721 gcaggagatt gccttagagg atctgcagca gtacccgatc ctgatgctcg accgcaagag
      781 caccaccagc gaatttttgc ataacctctt tctgcagcac cagcttgaac tgatgccgca
      841 gatcgagctg agcagcaacg atcttctgat tgatctggca cgtatcggcc tgggaatcgc
      901 gtttatcccg gattactgcc taaaccgcga gtccaaagag ctctttatcg tgaagatcaa
      961 ggaaaagctc ccggcacgcc agatggtcac cgcagtcaac ccgacgcttc caatcgcgca
     1021 gtccacggaa gaattcttaa aactcctgcc gtctctccca gaactcggct aacatctgct
     1081 ccacttcatt tacattacat atccggcgct gttcccattc tcttgggaac agccctctgt
     1141 aatcactctt taaaaaccgc tcgtccaaca gttcaatcac tcccacatcc tctgtcgtcc
     1201 gtatcacgcg cccggctgcc tgcaatacct tgttcattcc cggaaagcgg tacgcatagt
     1261 caaagcccgc ccccgctctc tcatcataat aacgcattaa aatctcgcgc tcattgctga
     1321 tctgcggcaa tccggttccc acgatcaccg ccccgatgag ctgttctttt ttcagatcaa
     1381 tcccctctcc gaaaatacct ccgagcacac agaacgccac cagtgaaccg cctcgccgtt
     1441 tcgatgcctc accggaaaat gcctgtaaga aagcctcgcg ctccgcctca cgcatcccgg
     1501 tctgctgcat catgcaccaa agacacggct ctgcgtctct gggattttcc gcatcattgg
     1561 gatgctccgc aatttctgcc tcttcgggat gctccgcaat ctctgcctct tcgggatgct
     1621 ccacaatctc tgcattctct gctccttccg gaaaatactc ctgcggacgc atttcatccg
     1681 tctccccgcc ctctaaaaac acatcgtaga cttcctgcat catccggtag gacggaaaga
     1741 acaccatata gtttccctgc ttcgcgcgcg ctgttttttc tatatataat gcaatccgct
     1801 catactctgc cctgccgcgc ctcgtgtact tgctgctgac gtcatttcca aacagcagca
     1861 ggcgctgttc ctcggaaaac gccgtctgcg cgtagaccgc ataattatcc ctgcgggtgg
     1921 acagcaggct tttataatac tggatcggaa gcagtgtcgc cgagaaaaag atcgtcgcat
     1981 ttcccttgtc tatgcgctcc tgcagattga gggagggatc cacacagtaa aacttcagct
     2041 tgaagcgccc gtcctcctca tgctctgaat acacgacata atgctcgtcg accagctcgt
     2101 agatattcat gaaattgcgc aggtttaagt aaaattcatt tacttccctg cgctccggga
     2161 aatcgggact tttctgcaga tactcatcca gatctgccgc aaggcgcgtc agtgcaaaga
     2221 tcagcgtacc gatattttca tatacgacat agttttcgca ctcacgcttg tatcccagga
     2281 ggatgcggtt gcacttttcc agatgccgga tcacacgttc cgcacgtctg tcatgctttt
     2341 cctgataggg tttcagcagc tttttgaccg ccaagaaatc ttccttatag atcgccgcgc
     2401 tgtacatttc ccggctccgc tccacaagat tgtgcgcctc atccaccaga aaaatgtagt
     2461 ccccgcgcgt tccttccgca aagaagcgtt tcagatagac attcgggtca aagacataat
     2521 tgtaatcccc tatgatatca tccgcccagg ttgccgtatc cagcgacatc tcaaacggac
     2581 agacccggta ctcccgcgcc tgctctaaga tcacttccct ggtaaaaaca tcctccttct
     2641 gcaacagatt gtacaccgca tcgttcacgc ggtcgtagtg tcccttcgcg taagggcaat
     2701 gcactgggtt gcagtccatc tcctcgcaca gacacagctt ttccttcgcc gtcagccaga
     2761 tgatcttcgc ctgatacccc ctggtgcgca aaagttcaaa cgcttccttt gccaccgtgc
     2821 ccgtaatcgt cttcgccgtc agataaaaaa tcttgtcccc gagtccctca ccgaccgcct
     2881 tcaccgccgg aaagaccgtc gaaatggtct tcccgacacc ggtgggcgcc tggataaaca
     2941 gattcttccg gcgcgcgatc gtgcggtata cgtccgatgc cagttctttc tgtcctttcc
     3001 ggtatgcata gggaaactcc agctttttga tggaagcctg ccgcagattc tgccaggcga
     3061 actgaaaatc cgcccacttt ttatattcgt ccgtcagccg ggtgaaccac tccgccagct
     3121 ccgcaaacga aaatgtttcc ctaaagtatt tcagattccc cagaaacgcc gtctttgtca
     3181 gttcgattgt atccagattg cagtaggtca gctgcacgga gatctctgca agatcatgct
     3241 gcaaggcata gatataggca taacacatcg cctgcgcgcg atgcacgccc acgggttcat
     3301 ccagcgcatc cagattcatg tagatcccct tgatctcatc aatcgtcaca tgcatgcctt
     3361 cctccaccgc aagatggttg aagttatccg aaaaatcagg ctgtctctcc ccgtcgcacg
     3421 taatctcgat tccgtccgcc cgtccctcga tggcaagcgt gtaatctccc tcgctaagtt
     3481 cgatcttaag cggcacctcc gcatggtaat tcgcgccttt gctcttctga atgcgcttat
     3541 ggatacggct gccctcccac atggcctccg gggacgcagt tctcccgtgg cggttatcaa
     3601 tgtctccctc ccgcagaatg aactccacca gattccgcac cgagatcttt atgattttct
     3661 tttcgtcttt ttcctgttgt tcatccatac ggcagcgctc cctactttcc ggtttcttat
     3721 catacagtgt aacacaaaaa cagggctgct gcacgaagag gtttctctcc ggcagcagcc
     3781 ctgtctccgt ctgtctgact agcagactgc atatttctgt accgtattct caaacccagt
     3841 gttctgtccg ccgtatttta cggtcagatc cttgctgagt ccaagataca gcattccgag
     3901 cagtgatttt gcatcaatca cagctctgtt ataacaaaca tcgatatcaa aatcacattt
     3961 tccggcagct ctcacaaact cctttacttc ctccgtgtca gacaatctga tttttctctc
     4021 tatcatatta ttcatccttt cctactataa accaaacgtg tttctctgat ttccgcgcaa
     4081 ttattgcata attttttttc tttgtcaaat ttgacgcaac cggtaaacca cttgaaagta
     4141 cgggcaatct gctcatttct gtgcccgatt tttgccg
//
LOCUS       DBMDQJ010000137         4167 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7974_length_4167_cov_1.605755, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000137 DBMDQJ010000000
VERSION     DBMDQJ010000137.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4167)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4167)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4167
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7974_length_4167_cov_1.605755"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="ACZO9R_03925"
     CDS             complement(<1..1151)
                     /locus_tag="ACZO9R_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYG1 family protein"
                     /protein_id="HIR0912250.1"
                     /translation="MTEDLLEQIRRKDAAAFTHGGKFHADDVFSAALLLYLNPEIVIT
                     RGNRVPEDFDGIVFDIGRGRYDHHQKDSRVRENGVPYAAFGLLWEELGGAILGEELAA
                     EFDEAFVQPLDNNDNTGEKNELATLIGNFNPAWDAQGGSDAAFFQASSVAGMILENKF
                     ERYRGNERADRQIEEVLAAHERAVTSGETAERDAAILILPYFIPCQKRLSETGIAFVI
                     FPSNRGGYCIQPQKREYSLHYKCSFPECWLGLEKEELVEASGLESAVFCHKGGFLMTV
                     GELSDAVEACRISLDSFKDEKALVNVGGGEAADELLRELPGMTEAQIYHIPLPELPEL
                     EQDGSYGEVAMEKADWKLRNKDYVKQILQCRPEAVYVEGDVFVAYPVVHL"
     gene            complement(1148..2170)
                     /locus_tag="ACZO9R_03930"
     CDS             complement(1148..2170)
                     /locus_tag="ACZO9R_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR0912251.1"
                     /translation="MEAYYYSKMDKTKQAAYYAMRQGLLALEDAILMPRLESEDLYQV
                     FFLLRLDHPEIFWATGYKYRYYQESANLVFLPEYLFEKGKIREHQKAMKARVEKLARA
                     AQNLSEWEKEKYVHDFICENVHYDKLKKPYSHEIIGPLGQGVGVCEGIAKSVKVLCDA
                     LGVWCVIALCGNCPEKGIKYRHTWNIVKIGGQYYHLDATFDNTLGAGAQIRYDYFNLC
                     DKSVFRDHQPLIAPAPECTDAQHFYYREKKLSFTKVEDVYKRALQTAKKGKTFTFHWR
                     GGYLTKEVFGELLEQISGAGRERGKSARVSVNWQQAVIRFDYVEAGSAPEARVVMEEA
                     NEGEKE"
     gene            complement(2312..3700)
                     /locus_tag="ACZO9R_03935"
     CDS             complement(2312..3700)
                     /locus_tag="ACZO9R_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912252.1"
                     /translation="MEQTTAKKNKYEIDMCNGTIMDKLISFSLPLMLSGILQLMFNAV
                     DIIVVGRFSGSQALAAVGSTSALINVFTNLFIGISLGANVLTARFYAAGREKEVSETV
                     HTAITLALVSGIIMALVGLVFSKGALALMGTPDDVIGLSALYMRIYFLGMPFFMLYNY
                     GAAILRAVGDTKRPLLFLMIAGVINACLNMVLVIVFKLGVAGVAIATVISQMISCVLV
                     LRCLCRSESSYRLRFSELGMKGYYLNQIFSVGVPAGIQSTVINFSNVLLQSSVNSFGS
                     TAMAGYTAANNLLGFLYVSVNSITQACMSFTSQNYGVGKLKRMDRVLADCMILSVVVA
                     VVLGCICYGFGPEILQIYTEDAEVIACGMEILAYTTITYFLCGWMDLFPGALRGMGYS
                     AVPMVLSVIGTVGTRVVWIFGIFPYHRSLAILFISYPASWIITIVLQVICFYFVRRRV
                     HRRSQALAGQEA"
     gene            complement(3762..4031)
                     /locus_tag="ACZO9R_03940"
     CDS             complement(3762..4031)
                     /locus_tag="ACZO9R_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009203393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR0912253.1"
                     /translation="MYYVPDGTKQCGYYECKKCGNRFLSLQTMTRLPCPDCASEVDYE
                     IGPDETLADVTDTAVLLQKIEGEEEVAKMDALLSLAITGGDFNWI"
BASE COUNT          976 a         1295 c          902 g          994 t
ORIGIN      
        1 agatgcacca ccggatatgc cacaaataca tctccctcga cgtaaacagc ctccgggcgg
       61 cactgcagga tctgttttac ataatctttg ttccggagtt tccagtcagc tttttccatc
      121 gccacttcac cgtaactgcc gtcctgctcc agttccggca gctccggcag cgggatatga
      181 tagatctgcg cttccgtcat ccccggcagt tctcttaaaa gttcatccgc cgcctctccg
      241 ccgcccacgt ttacaagcgc cttctcatcc ttgaaactgt ccagactgat ccggcatgcc
      301 tccactgcat cggaaagttc cccgaccgtc atgagaaatc cgcccttatg gcagaaaacg
      361 gcgctctcta agcccgatgc ctccaccagc tcttcctttt ccagtccgag ccagcattcc
      421 gggaagctgc acttataatg gagagaatac tcccgcttct gcggctggat acagtatccg
      481 ccccggttcg acgggaaaat cacaaacgca atcccggtct ccgacaggcg tttctggcac
      541 ggaataaaat agggcaatat cagaatcgcg gcatcgcgct ccgccgtctc cccggacgtc
      601 acagcacgct catgcgccgc cagcacctcc tcgatctgac ggtcggcgcg ctcatttccg
      661 cggtagcgct cgaatttatt ttcgaggatc atgcccgcca cgctggacgc ctgaaaaaag
      721 gcagcgtcgc ttccgccctg cgcatcccat gccgggttaa aattgccgat gagcgtcgcc
      781 agctcgtttt tctccccagt gttgtcgtta ttatccaacg gctgtacaaa cgcctcgtca
      841 aattccgccg ccagctcctc cccgaggatt gctcccccga gctcctccca cagcagcccg
      901 aacgctgcat agggcacgcc gttctcccgc acccggctgt ctttctgatg atggtcgtac
      961 cgtcctcttc cgatgtcaaa cacgatcccg tcaaaatcct ccggcaccct gttgccgcgc
     1021 gtaattacaa tctccggatt caggtaaagc agcagcgccg cggaaaacac atcgtctgca
     1081 tgaaattttc ctccgtgtgt aaacgccgcc gcatcctttc tccgaatctg ttccagtaaa
     1141 tcttccgtca ttctttttct ccctcattcg cttcttccat cacaacacgc gcctccggcg
     1201 cactgcccgc ctccacataa tcgaaccgga tcaccgcctg ctgccagttc acgctgactc
     1261 tggcgctttt tcctctctcc ctgccagccc cggaaatctg ctctaacagt tccccaaaga
     1321 cctccttggt gagatacccg cctctccagt gaaaggtaaa cgtttttcct ttttttgctg
     1381 tctgaagcgc acgtttatag acatcctcca ccttcgtgaa ggaaagcttt ttctcccgat
     1441 aataaaaatg ctgtgcatcc gtacattccg gcgcaggcgc gatcaggggc tgatgatccc
     1501 ggaatacgct cttgtcgcag agattgaaat aatcgtaccg gatctgtgct cccgcaccga
     1561 gcgtgttgtc aaatgtagca tccagatgat aatactgccc gccgattttt acaatgttcc
     1621 acgtatggcg gtatttgatc cccttctctg ggcagtttcc gcacagagca atcacgcacc
     1681 agacgccaag cgcatcgcaa agcaccttta ctgactttgc gattccctca cagacgccca
     1741 caccctgtcc gagaggacct atgatctcat gggaatacgg ctttttcagc ttatcgtagt
     1801 gcacgttttc gcagataaag tcatggacgt atttttcttt ctcccactcg gagagattct
     1861 gcgccgcgcg cgccagtttt tccacccgcg ccttcatcgc cttctggtgc tctctgatct
     1921 tgcctttttc aaagaggtat tccggcagga acacaaggtt cgccgactcc tgataatacc
     1981 ggtacttgta gccggtcgcc cagaaaatct ccggatgatc cagccgcagc agaaaaaaca
     2041 cctgatagag atcctccgac tcaaggcgcg gcatcaaaat ggcatcctcc aacgccaata
     2101 gtccctgccg catcgcataa tatgccgcct gctttgtttt gtccattttg ctgtaataat
     2161 atgcttccat cgttgtctcc gtgtagttat atgagctgga ctatgagctg gggttcctgt
     2221 cggattccgg gattgggctt ttgcctcgat ccggcagagt tccgacagat gacctgtcac
     2281 tttccactgg gtttcctggc tgattgagga atcaggcttc ctgtccggcg agcgcctgac
     2341 ttctccggtg cactctccgt ctcacaaagt aaaaacagat tacctgcagg acaatcgtga
     2401 taatccacga cgctggataa gatataaaca gaatcgccag tgatctgtgg tatggaaaaa
     2461 tgccaaaaat ccacaccact ctcgtaccaa ccgttccgat gaccgacaac accatcggca
     2521 ccgcggaata tcccatgccg cgcagcgccc ccgggaacaa atccatccag ccgcaaagaa
     2581 aataggtgat cgtcgtgtat gcaaggatct ccataccgca cgcaattacc tcggcatcct
     2641 ccgtgtagat ctgtaaaatt tccggtccga acccatagca gatgcatccc aaaaccacag
     2701 cgaccacgac cgataagatc atgcagtctg ccagcacacg atccatgcgc tttaattttc
     2761 caacgccgta attctggctg gtaaagctca tgcacgcctg tgtgatggag ttcaccgaga
     2821 catacaagaa gccgagcaga ttgtttgccg ccgtgtagcc tgccatcgcc gtcgatccga
     2881 acgaattgac cgaggactgc aggagcacat tggaaaagtt aatgaccgta ctctggattc
     2941 ctgccggaac gccgaccgaa aagatctgat tcaagtagta ccctttcatg ccaagctcgg
     3001 aaaaccgcag ccggtaactg ctctccgacc gacagaggca ccgcagcacc agcacgcagg
     3061 aaatcatctg cgagatgacc gtggcgatcg ccacaccggc aacgccgagc ttgaatacaa
     3121 tgacaagcac catattcaag cacgcgttga taactcccgc aatcatcaaa aacagcagcg
     3181 gacgttttgt gtccccgacg gcgcgcagaa tcgccgcgcc ataattgtag agcatgaaaa
     3241 acggcattcc cagaaaataa atccgcatat aaagtgccga cagtccgatg acgtcgtccg
     3301 gtgtccccat cagcgccagc gctcccttgg aaaaaacaag tcccaccagc gccatgataa
     3361 ttccactgac cagtgccagg gttatcgcgg tatgtacggt ctcagacacc tctttctcac
     3421 gccccgccgc atagaaacgc gccgtcagta catttgcccc cagcgaaatt ccgataaaca
     3481 gattcgtgaa cacgttgatg agcgcactgg tcgatcccac cgctgccagc gcctggctgc
     3541 cggaaaacct gcccaccacg ataatatcta ccgcattgaa catcagctgt aaaatgcccg
     3601 acagcatgag cggcagcgag aaggaaatca atttgtccat aatcgttcca ttgcacatat
     3661 caatctcata tttattcttt tttgccgttg tctgttccat ctctgtcacc gctcctgttc
     3721 tgtgtcttat ctgtttcatc cacttcccgt tctatctttt cttaaatcca gttaaagtct
     3781 ccgccggtaa tcgcaagact taagagcgca tccattttcg ccacttcctc ctcgccctcg
     3841 attttctgca gaagcactgc cgtatctgtc acgtccgcaa gcgtttcatc cggcccgatc
     3901 tcataatcaa cctccgatgc acaatccgga cagggaagcc gcgtcatcgt ctgcaaggac
     3961 agaaaccgat tcccgcactt tttacattca taatatccgc actgctttgt gccatccggt
     4021 acataataca tgctaaaacc tccttttgcc tgtcactatg tatcccattt tagcatgatg
     4081 gcttttaaaa cacaaatgca gggtatgtct tggaggaaag acataccctg catgggggaa
     4141 taaaggaaaa atgattttat tagaggt
//
LOCUS       DBMDQJ010000138         4158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_7987_length_4158_cov_1.389450, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000138 DBMDQJ010000000
VERSION     DBMDQJ010000138.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7987_length_4158_cov_1.389450"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="ACZO9R_03945"
     CDS             complement(<1..254)
                     /locus_tag="ACZO9R_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008976281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HIR0912254.1"
                     /translation="MTYKAFLIKYAEIAIKGKNRYLFEDALVSQMNHALKKVDGTFHI
                     RKEQGRIYVEMEGEYDYDGAVEALQHVFGIVGICPVVLLE"
     gene            complement(266..1420)
                     /locus_tag="ACZO9R_03950"
     CDS             complement(266..1420)
                     /locus_tag="ACZO9R_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR0912255.1"
                     /translation="MEAYLDNSATTRCSERAKNKMVQILTEDYGNPSSLHNMGKTAED
                     YIKEAKEQIAKTLRCDEKEIIFTSGGTESNNMALIGGALANKRQGKHIITTAIEHASV
                     QAPLAYLKELGYEITELSVDHDGIISLEELRDAVREDTVLVSIMMVNNEIGAVEPVEE
                     AAKVIKAKNPDTLFHVDAIQAYGKFRICPKKMGIDLLSVSGHKIHAPKGTGFLYIRDK
                     VKVKPIIYGGGQQKGMRSGTENVPGVAALGEAAAEIYENFDAKIGHMYEIKQRFIDEV
                     TQIPGVTVNGRTGRDSAPQIVSVTIEDVRSEVMLHSLEEYGIYVSAGSACSSNKPAPS
                     RTLQGIGLPKQKLESTIRISFCVNTTEEEVDYAVEKMKEIIPFRRKFTRK"
     gene            complement(1463..2233)
                     /locus_tag="ACZO9R_03955"
     CDS             complement(1463..2233)
                     /locus_tag="ACZO9R_03955"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR0912256.1"
                     /translation="MYQFFVEDEQIANDLVIIEGSDVNHIRNVLRMKCGEKVRISSTS
                     GRNFFGTIDRIAENVVEVCITEETALDTELPCRICLFQGLPKSDKMELIVQKAVELGA
                     AEVIPVAMKNCVVKLDAKKAAAKTARWQEIAKSAAKQSKRSRIPVVREPVNYREAVAL
                     AGELDVVLLPYENERGMAATREVMERIAPGQSVGIFIGPEGGFDPSEIALAKEEGMHL
                     VSLGKRILRTETAGLATLSILMYHIESVSEAEEERDQP"
     gene            complement(2300..3286)
                     /gene="prmA"
                     /locus_tag="ACZO9R_03960"
     CDS             complement(2300..3286)
                     /gene="prmA"
                     /locus_tag="ACZO9R_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR0912257.1"
                     /translation="MKWNKYTIETTTAAEDYMSAMLADLGIEGVEIEDNIPLSKEDQA
                     DMFIDFLPELPPDDGVSHVSFYLEDDGEDRTELLKQVKEALEDLRKVVDVGSGVITSD
                     QTEDLDWINNWKKFFSSFYIDDILIKPTWEELKPEDADKFLIEIDPGVSFGTGKHETT
                     QLCIRQLLKYIRGNDAYTPENRTPKVLDVGCGSGILSIVALKLGASAVVGTDLDPDCM
                     TSTHENMEVNHLDPSLGTFYVGNLIDDAALQEKVGTEEYDIVVANILADVIIPMAPVI
                     PARLKRGGYFITSGIIDFKENAVKEAIEAAGLTVVEINHQGEWVNITARKPE"
     gene            complement(3495..>4158)
                     /locus_tag="ACZO9R_03965"
     CDS             complement(3495..>4158)
                     /locus_tag="ACZO9R_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR0912258.1"
                     /translation="STTIKKGDVLALIGPSGCGKSTFLRSLNLLEIPTAGHVLFEGTD
                     LTDPSVDINHVREKIGMVFQQFNLFPNMTILENIMLAPVKLGKMTKEEANTRAMELLK
                     RIGLADKAGAYPAQLSGGQKQRIAIVRSLAMDPDIILFDEPTSALDPEMVGEVLAVMQ
                     ELAEDGMTMVVVTHEMGFAREVANRVMFINDGVIQEEGTPQEIFGNPKSQRLQEFLSK
                     VL"
BASE COUNT          847 a         1310 c          881 g         1120 t
ORIGIN      
        1 tcctccagca gtacgaccgg acagattccc acgatgccaa acacatgctg cagtgcctcc
       61 acagctccat cgtaatcgta ttcgccctcc atctctacat agattctgcc ctgctccttg
      121 cggatatgga acgtgccgtc caccttcttt aaggcatgat tcatctggct caccagagca
      181 tcctcaaaca ggtagcggtt ctttcccttg atggcaatct ctgcatattt aatcaaaaat
      241 gctttatatg tcatggtttt ctcctttact ttctggtgaa ctttctgcgg aacggaatga
      301 tctctttcat tttttccacc gcgtaatcca cttcttcttc cgttgtattg acacagaagc
      361 tgatccggat cgtggactct aacttctgct tcggcagacc gatgccctgc aaggtgcggc
      421 tgggtgccgg cttattggac gagcatgcgc ttcccgccga cacatagatg ccgtattctt
      481 ccagagaatg gagcatcact tcacttcgca catcctcgat cgtgacactc acaatctgcg
      541 gcgcagagtc ccttcccgtt ctgccgttta ccgtcactcc cgggatctgt gtcacctcat
      601 cgatgaacct ctgcttgatc tcatacatat gaccgatctt cgcgtcaaag ttctcataga
      661 tctccgccgc tgcctcgccg agtgcagcca cgcccggaac attctccgtg ccggagcgca
      721 ttcccttctg ctgaccgccg ccgtagatga tcggttttac ttttaccttg tcgcggatat
      781 ataaaaatcc ggttcctttc ggcgcgtgga tcttgtgtcc actcaccgag agtagatcaa
      841 tccccatctt tttcgggcag atgcggaatt tgccgtacgc ctgaatcgca tccacatgaa
      901 acagcgtgtc cggattcttc gccttgatga ccttcgccgc ttcctcgacc ggttcaaccg
      961 caccaatctc gttgttcacc atcatgatgg acaccagcac cgtatcttcc cgcactgcat
     1021 cccgcagttc ctccagcgaa ataataccgt catgatccac agacaattcc gtgatctcat
     1081 agccgagttc ttttaagtag gcaagcggcg cctgtaccga cgcatgctca atcgctgtcg
     1141 tgatgatatg ctttccctgg cgcttgtttg ccagtgctcc gccgatcagc gccatattgt
     1201 tcgactccgt gccacccgac gtaaagatga tctccttctc gtcacagcgg agcgtcttcg
     1261 cgatctgctc ttttgcttct ttaatatagt cctccgccgt ctttcccatg ttgtgcaggg
     1321 aggacggatt gccataatct tccgttaaga tctgcaccat cttgtttttg gcgcgctcac
     1381 tgcaccgggt ggtggcagaa ttatccaaat atgcttccat ctgttttaaa accatgcctt
     1441 tctaacaaac gccgacgcag aatcacggct gatcccgctc ttcttccgct tccgacacac
     1501 tctcgatatg atacatcaga atagaaaggg ttgcaagtcc cgctgtctcc gtgcgcaaaa
     1561 ttctttttcc gagggacacc agatgcatcc cctcctcttt tgccagcgca atctccgacg
     1621 gatcgaatcc gccctccggt ccgatgaaaa tgccgacgct ctgccccggt gcaatccgct
     1681 ccatcacttc ccttgtggcc gccatgccgc gctcattctc atacggcagc agcaccacgt
     1741 cgagttctcc cgccaatgcg actgcctccc ggtaattcac cggttctctc accaccggaa
     1801 ttctgctgcg ctttgactgt tttgccgcac tctttgcaat ctcctgccag cgcgccgtct
     1861 ttgccgccgc tttttttgca tccagtttta cgacgcagtt cttcatcgcc accgggatca
     1921 cctccgcagc gccaagctcc actgccttct ggacgatgag ctccatcttg tcgctctttg
     1981 gaagtccctg aaacaggcag atccggcagg gcagttccgt atctaaagcc gtctcctccg
     2041 tgatgcacac ctccaccacg ttctctgcga tccggtcaat cgttccgaag aaattcctgc
     2101 cggatgtgct gctgatgcgc actttttccc cgcatttcat gcgcaggaca ttgcggatgt
     2161 gatttacatc gcttccctca attatcacca gatcatttgc aatctgctcg tcttccacaa
     2221 aaaactgata cataaactcc tcatttccgg gctgcctgcc attccttcgg atcatcttcc
     2281 ttttttcgga tcgtcttcct tattccggct ttctcgccgt aatgttcacc cactcgccct
     2341 gatgattgat ctccaccacc gtaagtccgg ccgcttcgat cgcttctttt accgcgttct
     2401 ccttgaaatc gatgatgccc gaggtaataa aataaccgcc gcgtttcaag cgtgccggaa
     2461 tcaccggtgc catcggaata atgacgtctg ccagaatgtt ggcaaccaca atgtcgtatt
     2521 cctccgtgcc gactttctcc tgcagtgctg catcatcgat cagattgccg acataaaatg
     2581 tgccaagcga aggatcgaga tggttcactt ccatattctc atgtgtggag gtcatgcagt
     2641 ccggatcgag atccgttccg actaccgcgg acgcgccaag cttcaacgcc acaatcgata
     2701 aaatgccgct tccgcagccg acgtcgagta ccttcggcgt gcggttttcc ggtgtgtacg
     2761 catcgttccc gcggatatac tttaacagct ggcggatgca cagctgcgtc gtttcatgct
     2821 tgccggttcc aaacgacaca cccggatcaa tctcgattaa aaatttgtcg gcatcctccg
     2881 gctttaattc ctcccaggtc ggtttgatca ggatgtcatc gatatagaag gaactgaaga
     2941 atttcttcca gttgttgatc cagtccagat cctccgtctg gtcggaggtg atcacgccac
     3001 ttccgacatc cacgactttt cttaagtcct ccaacgcctc ttttacctgc tttaacagtt
     3061 cggtgcggtc ctcgccgtca tcctcgagat aaaagctgac gtggctcaca ccgtcatccg
     3121 gcggaagctc cggcagaaaa tcgatgaaca tatccgcctg atcttccttg gacagcggaa
     3181 tgttatcctc aatctccact ccctcaatgc caagatcggc taacatcgca ctcatgtagt
     3241 cctccgccgc cgtcgttgtc tctatcgtgt atttattcca tttcatatgc gttcttcctt
     3301 cccttacgat tccgttccta aaaatcgtta tttatcttat catacaatcg cacatcttgc
     3361 aaacgatgtt ccgccccgct ctccgcctgc gggaggtatt cctgatgcag tatacaaaac
     3421 acattctttg cctgttgcac aaaaaacggg ccgcggcacc tcttttgcgc cggacagccc
     3481 gtccttattt cttcttacaa tacctttgat aaaaactcct gcagtctctg actctttgga
     3541 ttgccaaaaa tctcctgcgg ggttccctcc tcctggatca cgccgtcgtt gataaacatc
     3601 acacggttcg caacttctct ggcaaatccc atctcatgtg tcacgaccac catcgtcatg
     3661 ccatcctccg ccagttcctg catcacggcg agcacctcgc cgaccatctc cggatcaagc
     3721 gccgaggtcg gctcgtcaaa caggatgatg tccgggtcca tcgccagcga ccgcacgatg
     3781 gcaatgcgct gcttctgtcc gccggaaagc tgcgcgggat acgcgcccgc cttatccgcc
     3841 agaccgatgc gctttaacag ctccatcgcg cgcgtattcg cttcctcttt tgtcatcttg
     3901 ccgagtttta ccggggcaag catgatattt tccagaatcg tcatgttcgg aaacagattg
     3961 aactgctgaa acaccatgcc gatcttctcc cggacatgat tgatgtccac cgacggatcc
     4021 gtcagatcgg ttccctcaaa cagcacatgt cccgccgtcg gaatctccag cagatttaag
     4081 gagcgcaaaa acgtggattt accacagccg gacggtccga tcagcgccag cacatcgccc
     4141 tttttgatcg tcgtcgag
//
LOCUS       DBMDQJ010000139         4129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8057_length_4129_cov_1.416584, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000139 DBMDQJ010000000
VERSION     DBMDQJ010000139.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8057_length_4129_cov_1.416584"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZO9R_03970"
     CDS             complement(<1..213)
                     /locus_tag="ACZO9R_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912259.1"
                     /translation="MRRWIKQIMVWLAAAACGIVAALVICRWSGTAFGLFMWPVIAAG
                     AAVGGKKEGEGPLAAEFDELSADNRFG"
     gene            complement(244..1602)
                     /locus_tag="ACZO9R_03975"
     CDS             complement(244..1602)
                     /locus_tag="ACZO9R_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR0912260.1"
                     /translation="MLEKWFKLKENNTTAKTEVMAGITTFMTMAYILAVNPTMLAAAG
                     MDKTAVLIATALASFIGTMAMALLANYPFALAPGMGLNAYFAYTVCGAMGYSWQVALM
                     AVFVEGLVFIVLSLTNVREAIFNAIPLNLKKGVSVGIGLFIAFIGLQNGGIVVANSST
                     LVSYVDFTSNFKTAGICALLALIGLFVIAIMYIKNVKGAILFGIVITWVLGMICQAVG
                     IYVPDAEAGFYSLYPALGFTDLTAIGQTFGQCFKLDFSNVRVFDFIAVVCAFLFVDMF
                     DTLGTLIGVANKADMLDKDGKLPRIKEALLADAVATTAGAVLGTSTTTTFVESSSGVA
                     AGARTGLSSVVTGFLFLISVVLSPIFCSIPSFATAPALIFVGFLMVSTVTSIEFTDMT
                     EAIPAYLCLLAMPLMYSISEGIAIGVISYVIINVVCGKAKKITPLMYVLAVLFVCKYI
                     FL"
     gene            complement(1624..2835)
                     /gene="ilvA"
                     /locus_tag="ACZO9R_03980"
     CDS             complement(1624..2835)
                     /gene="ilvA"
                     /locus_tag="ACZO9R_03980"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR0912261.1"
                     /translation="MLTLDKFEEASEKVKEVTLETKLVYSDYWSQQTGNKVYLKPENM
                     QFTGAYKVRGAYYKISTLSDEERAQGLITASAGNHAQGVAYAAKCYGCKAVIVMPTTT
                     PLIKVNRTKSYGAEVVLYGDVYDEACQKAYELADKEGYTFIHPFDDLAVATGQGTIAM
                     EIFKELPLVEYILVPIGGGGLATGVSTLAKLLNPKIKVIGVEPAGANCMQASFAAGKV
                     TTLPQVNTIADGTAVKTPGSKIFPYIQKNLDDIITVEDDELVVAFLDMVENHKMIVEN
                     SGLLTVAALKHLDVSDKRVVSILSGGNMDVITMSSVVQQGLIFRDRIFTVSVLLPDKP
                     GELSKVSAAIAAEQGNVIKLEHNQFVTTNRSAAVELRITLESFGTEHKKQIMKALEKK
                     GYKPKLVRTSL"
     gene            complement(2829..3776)
                     /gene="ftsY"
                     /locus_tag="ACZO9R_03985"
     CDS             complement(2829..3776)
                     /gene="ftsY"
                     /locus_tag="ACZO9R_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR0912262.1"
                     /translation="MAEEKKGFFRRLADGLAKTRNNIVSGMDAIFNGFSDIDDDFYEE
                     IEEILIMGDIGVNATESIIESLKEKVKENHIKDPAECKELLINSIKEQMDVGETAYRF
                     ETEKSVVLVIGVNGVGKTTSVGKLAGKLKDQGKKVVLAAADTFRAAAGDQLQEWANRA
                     GVDMIGGQEGADPAAIVYDAVAAAKARNADVLLCDTAGRLHNKKNLMEELKKINRILE
                     KEYPDAYRETLVVLDATTGQNALNQAREFSECADITGIILTKMDGTAKGGIAVAIHSE
                     LGIPVKYIGVGETIEDLQKFDSNQFVNALFEVGEEKSGC"
     gene            complement(3788..>4129)
                     /locus_tag="ACZO9R_03990"
     CDS             complement(3788..>4129)
                     /locus_tag="ACZO9R_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912263.1"
                     /translation="RIISQPPGKKLQNMMQLSGGEKALTAISLLFAIQNLKPSPFCLL
                     DEIEAALDDSNVVRFAKYLHKLTSDTQFIVITHRRGTMNAADRLYGITMQEKGVSTLV
                     SVNLIENDLDK"
BASE COUNT          945 a         1217 c          928 g         1039 t
ORIGIN      
        1 gccaaagcgg ttgtctgcgc tcagttcatc gaactctgca gccagcgggc cttcgccctc
       61 ctttttgccg cccactgccg cccccgcagc aatgaccggc cacataaaaa gaccgaaggc
      121 agtgccgctc catctgcaga tcacaagcgc cgcaacaata ccgcatgctg ccgcagccag
      181 ccagaccatg atctgtttta tccatctgcg catagggata cctccatacc ttcggttttc
      241 attttataag aagatgtatt tgcacacaaa cagtaccgcc agcacataca tcagcggtgt
      301 gatcttcttt gccttaccgc atacgacatt gatgatgacg taggagataa cgccgatggc
      361 aataccctcg gagatgctgt acataagcgg cattgcaagc aggcataagt atgccggaat
      421 tgcctccgtc atatccgtga actcgatgct tgtcaccgta gacaccataa ggaagccgac
      481 gaagatcaga gccggtgctg tcgcaaagct cgggatcgag cagaaaatcg gggacagtac
      541 cacagagatc aggaatagga atccggtcac tacagaggag agacctgttc ttgctccagc
      601 tgccacaccg gaggaactct caacaaacgt ggtggttgtg gaggtaccga gcacagcgcc
      661 tgcggtggtt gcaaccgcat ccgccagcag cgcttccttg atgcgcggca gcttgccgtc
      721 cttatccagc atatccgctt tgtttgccac accgattaac gttccgagtg tatcaaacat
      781 atctacaaac agaaatgcac atacaactgc gatgaagtca aagacacgca cattggagaa
      841 atccagcttg aagcactgtc cgaatgtctg accgatcgcg gtcagatccg taaatcccag
      901 cgccgggtat aaggaataaa aaccggcttc cgcatccggc acatagattc cgactgcctg
      961 gcagatcatt ccaagcaccc aggtaataac gataccgaat aaaatcgcgc cttttacgtt
     1021 cttaatgtac ataattgcaa tcacaaacag accgatcagc gctaacaaag cacagatgcc
     1081 ggcagtcttg aaattgctgg taaagtcaac ataggaaaca agtgtggagc tgttcgccac
     1141 tacgatgccg ccattctgca gaccgataaa tgcgataaac agcccgatgc cgacgctcac
     1201 accttttttc aggttcagcg ggatcgcatt aaagattgct tcacgcacat tggtcaaaga
     1261 aagtacgatg aaaaccagac cttccacaaa aacagccatt aaagctacct gccaggaata
     1321 tcccatcgca ccgcagaccg tgtatgcaaa atacgcattc agtcccatcc ccggtgccag
     1381 tgcaaacgga taatttgcaa ggagcgccat cgccatggta ccaataaatg atgccagcgc
     1441 ggttgcaatg agcactgctg tcttatccat tcccgccgct gcaagcatgg tcgggttgac
     1501 tgccagaatg tacgccatcg tcataaaggt tgtgatacct gccatgactt cggtcttggc
     1561 tgtcgtgttg ttttccttca gtttaaacca tttttctaac attttctttc tcctcttctt
     1621 tgtttacaga ctcgtgcgca ccagcttcgg cttataacct ttcttctcca gcgctttcat
     1681 gatctgcttt ttgtgctcgg tgccgaagct ctcgagcgtg atacggagtt ccaccgcagc
     1741 actgcggttc gtagtcacaa actggttgtg ctccagcttg atgacgttgc cctgctctgc
     1801 ggcaatcgct gccgaaacct tggacagttc tcccggctta tccggcagaa gtaccgacac
     1861 ggtgaagata cggtcgcgga aaatgagtcc ctgctgtacc acggaggaca tcgtgatgac
     1921 gtccatgttg ccgccgctta agatcgatac cacgcgtttg tcggaaacat ccagatgttt
     1981 caatgcagct acggtcagca gaccggagtt ttccacaatc atcttgtgat tctccaccat
     2041 atccaggaat gccacgacga gttcatcatc ctccaccgtg atgatgtcat ccagattctt
     2101 ctggatatac gggaaaatct tgcttcccgg cgtcttgacc gccgtgccgt ccgcaatggt
     2161 gttgacctgc ggcagcgtcg ttacttttcc cgccgcaaag gaagcctgca tacagttcgc
     2221 tcccgccggc tccacgccga tgactttgat cttcggattc agaagcttcg ccagtgtgga
     2281 cacgccggtt gccagtccgc cccctcctat cgggaccaga atgtactcca ccagcggaag
     2341 ctccttaaag atctccatgg cgatcgttcc ctgtccggtt gccactgcca gatcatcgaa
     2401 cggatggata aaggtatatc cctccttgtc cgcaagctcg tatgccttct ggcacgcctc
     2461 gtcatagaca tctccgtaaa gcacgacttc tgcgccgtag ctcttggtgc ggttcacctt
     2521 gatgagcggt gtggtggtcg gcatgacgat gaccgccttg cagccgtagc attttgctgc
     2581 gtatgccaca ccctgcgcgt ggtttcccgc cgatgccgtg atcagcccct gtgcacgctc
     2641 ctcatcactt aacgtgctga tcttataata tgcgcctctc accttgtatg ccccggtgaa
     2701 ctgcatattt tccggcttaa ggtaaacctt gttgccggtc tgctgactcc agtaatcgct
     2761 gtataccagc tttgtctcaa gtgtcacttc tttgactttt tcacttgctt cctcgaactt
     2821 gtctaatgtc aacatccgct tttctcctct cctacttcaa ataatgcgtt tacgaactgg
     2881 ttggaatcaa acttctgcag atcctcgatc gtctccccga cgccgatata cttgaccgga
     2941 atgccaagct ccgagtggat tgccactgcg ataccgccct ttgccgtgcc gtccatcttc
     3001 gttaaaataa ttcccgtgat gtcggcgcac tcggaaaact ctctcgcctg gtttaacgca
     3061 ttctgtccgg tcgtcgcatc gagcaccaca agcgtctcac ggtacgcatc cggatattct
     3121 ttctccagaa tacgattgat cttttttaat tcctccatga ggttcttctt attatgaagg
     3181 cgtcccgccg tatcacacag cagaacatcc gcatttctcg ccttggctgc cgccaccgca
     3241 tcgtagacga tcgccgccgg gtctgcgccc tcctgtccgc cgatcatatc cacaccggcg
     3301 cggtttgccc actcctgcag ctggtctccc gccgctgcgc ggaaggtatc cgctgccgca
     3361 agcacgacct ttttcccctg gtccttgagc tttcccgcca gctttccaac ggatgtggtc
     3421 ttgcccacgc cgttgacacc gatcacgaga accacggatt tctccgtctc aaagcggtat
     3481 gccgtctcgc cgacatccat ctgctccttg atgctgttaa tcagaagttc cttgcactct
     3541 gccggatctt tgatgtgatt ctccttcact ttttccttga gactctcaat gatcgactca
     3601 gtcgcattga cgccgatgtc tcccataatc agaatctcct cgatctcctc atagaaatca
     3661 tcgtcaatgt ccgagaaacc gttgaaaata gcatccattc cagatacaat attgtttctc
     3721 gtctttgcca ggccatctgc cagacgtctg aaaaatcctt ttttctcctc agccataagg
     3781 ccacctccta tttatccaga tcattttcga tcagattcac tgacaccaga gtagatacac
     3841 ccttctcctg catagtaata ccatacagac ggtcagctgc attcatggta cctcttcggt
     3901 gcgtgatcac gataaactgc gtatcgctgg tcagcttgtg aaggtatttt gcaaaacgta
     3961 ccacattgga gtcgtccagt gccgcctcga tctcgtccag aagacagaag ggggacggct
     4021 ttaagttctg aatggcaaac agaagagaga tggctgtcag agccttctca ccaccggaaa
     4081 gctgcatcat attctgtagc ttctttcccg gcggctgcga gatgatccg
//
LOCUS       DBMDQJ010000140         4094 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8140_length_4094_cov_1.765840, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000140 DBMDQJ010000000
VERSION     DBMDQJ010000140.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8140_length_4094_cov_1.765840"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(108..923)
                     /gene="larE"
                     /locus_tag="ACZO9R_03995"
     CDS             complement(108..923)
                     /gene="larE"
                     /locus_tag="ACZO9R_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HIR0912264.1"
                     /translation="MEQMEALKQSIREKEKMVVAFSGGVDSTFLLKVAHDVLGDGAVA
                     VTARSCSFPERELREAKAFCEKEHIKHIVVESEELQIEGFSHNPTNRCYLCKKELFTK
                     IWDIAKEYDIPYVAEASNLDDNGDYRPGLMAVAELSVLSPLREAGMNKSMIRELSHGL
                     GLPTWNKQSFACLSSRFVYGEEITEERLGMVDRAEQLLLDAGFHQVRVRIHESGTNTI
                     ARIEIGTEDFAKMFADDFYQKVNAACKEIGFTYVTLDLGGYRMGSMNDGIRKA"
     gene            complement(955..1782)
                     /locus_tag="ACZO9R_04000"
     CDS             complement(955..1782)
                     /locus_tag="ACZO9R_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR0912265.1"
                     /translation="MSRVLSYEEKDTWIHRLSGVTKLLFFLLWSITSMISYDTRVLLV
                     MLVCSLIIFAMSKTKWKQVGTVFKFIMFFLVINLIAIFFFSPGQGTKIYGTETPIVHL
                     FGHYTLTKEQLFYEFNVMVKYLTVIPAVFMFIVATNPSELAASLNRVGVSYKIGYSVA
                     IALRYIPDVQDDFAKIKHAQEARGIEMSGKAKLGDRIKNVAAIIFPLVFSSMDRIDVV
                     SNAMELRGFGKHKKRTWYMGKPLAAADYAVLIVTAAFSAVALMITFSNGSRFYNPFV"
     gene            complement(1782..3506)
                     /locus_tag="ACZO9R_04005"
     CDS             complement(1782..3506)
                     /locus_tag="ACZO9R_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912266.1"
                     /translation="MEPIIEFQNFSFKYRSQKEPTLKDINLAIYPGEKVLIVGPSGSG
                     KSTLAHCINGLVPFSYAGDITGTYRIKGEDPAKRGIFGLSKSVGTVLQDTDGQFIGLT
                     VAEDIAFALENDRVEYHEMRGRVDKAADAVDVKDLLDHAPGALSGGQKQRVSMAGVLV
                     CDVEILLFDEPLANLDPATGKRTIALIDELQKAKGATILIIEHRLEDALYRDVDRIIV
                     VGEGKIVADTTPDELLAANVLAEEGIREPLYITALKHAGCEIRPETHPAHVETMELSG
                     YKEALCRWNEKVALPEKKPGDDVILSVENLAFSYDGRTPILTDIHMQVQKGEMLAIVG
                     KNGAGKSTLSNLICGFLKPGQGRILFGGEDIADWSIKERGEKIGLVMQNPNQMISKPM
                     IYDEVALGLAVRGVPEEEIKERVYATLKICGLYQFRNWPVSALSFGQKKRVTIASILV
                     LHPQVLILDEPTAGQDYRHYTEIMEFLKKLNEEYGITIIMITHDMHLMLEYTNRAVVI
                     ADGRLLADTAPAAVLTDDAVADRAYLKKTSLYDLAVRCGIAEPTQFVERFIGYERQMR
                     AAEREEEA"
     gene            complement(3664..>4094)
                     /locus_tag="ACZO9R_04010"
     CDS             complement(3664..>4094)
                     /locus_tag="ACZO9R_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HIR0912267.1"
                     /translation="ALLAFLAAVFGPVVGGIVGLLGHAIGDALFYGSVWWSWVFPDAV
                     FGILVGLFAAKYAIKDGGFEKKQIVLFNVVQVVANLAAWVIVAPVLDIVIYAEPANKV
                     FAQGAWACLGNVIIIGILGTLIAAGYSKIGAKSSSLSKED"
BASE COUNT          955 a         1240 c          903 g          996 t
ORIGIN      
        1 ttaaataatt tctttttgtt ctaaaaaaga aacagaagca ttctgtgtca gagaatcctt
       61 ctgttttgtt ctgttactct atttctatat tctatgcagt tttaatatta tgctttccgg
      121 atgccgtcgt tcatgcttcc catccggtat ccgccgagat cgagtgtcac ataggtaaaa
      181 ccgatctcct tgcatgccgc attgaccttc tggtaaaaat cgtctgcaaa catttttgca
      241 aaatcctccg tgccgatctc gatacgggca atcgtgttcg ttccgctctc gtgaatccgc
      301 acgcgcacct gatgaaatcc ggcatccagc agaagctgct ccgcgcggtc caccatgcca
      361 aggcgctcct cggtgatctc ctccccgtag acaaagcggg aagaaagaca tgcaaacgac
      421 tgcttattcc atgtcggaag ccccagcccg tgggagagtt cgcggatcat gctcttgttc
      481 atccccgcct cgcgcagcgg actaagcacg gaaagctcgg caaccgccat cagtcccggg
      541 cggtaatcgc cgttgtcgtc cagattggac gcctccgcca cgtaggggat gtcgtactcc
      601 tttgcaatat cccagatctt tgtgaaaagc tccttcttgc agaggtagca gcggttggtc
      661 ggattatggg aaaatccctc aatctgcaat tcctccgact ccaccacgat atgttttatg
      721 tgctccttct cacagaatgc ttttgcctct cttaattctc tctccggaaa ggaacaggag
      781 cgagccgtca ctgcgaccgc cccatctccc agcacgtcat gtgccacttt taataagaat
      841 gtcgaatcca caccgccgga aaatgcaaca accatcttct ctttttcccg gatcgactgt
      901 tttaatgctt ccatttgttc catcgtattc taaacccgct ttcttccatt ttaattacac
      961 aaacggattg taaaaacggc ttccgttgct gaaggtaatc atcagtgcca ccgcggaaaa
     1021 tgccgccgtc acaatcagca ccgcatagtc tgccgccgcc agcggctttc ccatatacca
     1081 ggtgcgcttc ttgtgctttc cgaacccacg gagctccatc gcattgctga ccacgtcgat
     1141 gcgatccatg ctggagaata ccagcgggaa aatgatcgcc gccacgttct tgatgcggtc
     1201 gccgagcttc gccttgccag acatctcaat tcccctcgcc tcctgggcgt gcttgatttt
     1261 tgcaaaatca tcctgcacat ccggaatata ccggagcgca atcgccacgg agtaaccgat
     1321 cttgtagctg acaccaacac ggtttaaaga agcggccagt tcgctcggat tcgttgccac
     1381 gataaacatg aacaccgctg gaatgaccgt caggtatttt accatcacgt tgaactcata
     1441 aaacagctgc tcttttgtca gcgtgtaatg tccaaacaga tgcactatcg gcgtctccgt
     1501 tccgtaaatc ttcgtcccct gtcccggaga aaagaagaaa atggcgatga gattgatcac
     1561 cagaaaaaac atgatgaact tgaataccgt accgacctgc ttccacttcg tcttcgacat
     1621 ggcaaaaata atcaggctgc acacaagcat gaccaagagc acccgcgtat cgtaggagat
     1681 catgctggtg atcgaccaca ggaggaaaaa caaaagcttt gtcaccccgc tcagacggtg
     1741 aatccatgtg tctttttctt catatgataa tacgcgcgac attatgcttc ctcctctctt
     1801 tccgccgctc tcatctgccg ctcgtagccg ataaagcgct ccacgaactg tgtcggctct
     1861 gcaatgccgc agcgcaccgc cagatcgtag agagatgttt tcttcaaata ggcacgatcc
     1921 gccaccgcgt catccgtgag taccgccgcc ggagcggtat ctgccagaag cctgccgtca
     1981 gcaatcacga ccgcgcggtt cgtatactct aacatgagat gcatgtcatg tgtgatcatg
     2041 atgatcgtaa tgccgtactc ctcattcagc ttctttaaaa actccatgat ctccgtataa
     2101 tgtctgtagt cctgtcccgc cgtcggctcg tccaaaatca gcacctgcgg atgcagcacg
     2161 aggatcgaag cgatcgtcac gcgcttcttc tgaccgaagc ttaacgcgga gaccggccag
     2221 ttgcggaact ggtagaggcc gcagatcttt aaggttgcat agacccgctc cttaatctcc
     2281 tcctcgggca cgccgcgcac cgcaagtccc agcgccacct catcatagat catcggtttg
     2341 ctgatcatct gattcggatt ctgcatgaca agtccgatct tctcgccgcg ctccttgatc
     2401 gaccagtctg caatgtcctc gccgccaaac agaatccgcc cctgaccggg ctttaaaaag
     2461 ccgcagatga gattcgagag cgtcgacttt ccggcaccgt tcttgccaac aatcgccagc
     2521 atctcgccct tttgtacctg catatggatg tcggttagaa tcggcgttct tccgtcatag
     2581 gaaaaagcaa gattctccac cgagaggatg acgtcatccc ccggcttttt ctccggcagt
     2641 gcaaccttct cgttccagcg gcacagcgcc tccttataac cggacaattc catcgtctcc
     2701 acatgcgccg gatgcgtctc gggacgtatc tcgcatcccg catgcttaag cgccgtgatg
     2761 tagagtggct cgcggattcc ctcctcagcc agtacattgg ctgccagaag ttcatccggc
     2821 gtcgtatccg ccacgatctt tccctcaccg acgacgatga tgcggtccac atcccggtac
     2881 agtgcatctt ccagacgatg ctcaataatc aggatcgttg ccccttttgc cttctgtagc
     2941 tcatcgatca aagcgatcgt gcgctttccc gtcgccgggt ccagattggc aagcggctcg
     3001 tcaaaaagca gaatttccac atcgcagacc agcacgcccg ccatggatac gcgctgcttc
     3061 tgtccaccag acagcgcccc cggcgcgtga tcgagcaggt cttttacatc cacagcgtcc
     3121 gccgctttgt ccacgcgccc gcgcatctcg tgatattcca ctctgtcatt ctccagcgca
     3181 aatgcgatat cctccgccac cgtaagcccg atgaactgac cgtccgtgtc ctgcaaaacc
     3241 gtacccacag acttcgacaa tccaaaaata ccacgctttg caggatcctc gcccttaatg
     3301 cggtaagtcc ccgtgatgtc tccggcatag gaaaacggca caagcccgtt gatgcagtgc
     3361 gccagtgtcg actttccgga accggacggt ccgacaatca aaaccttctc ccccggataa
     3421 atggcgagat taatgtcctt tagcgtcggt tccttctggg aacgatattt aaaagaaaaa
     3481 ttctgaaatt ctatgatcgg ttccattccg aaatcctcgt cttcctctct tgtggtactt
     3541 cccgactatt atacttgata ataattccaa atggaagaaa aagtgcgcgg aaccggtcac
     3601 attttgcacc ccgtctccgc gcactctgtc tgcttctgtc tttctgctgc tgccggaatc
     3661 cgcctagtcc tccttggaga gactggacga ttttgctccg atcttggagt atcctgctgc
     3721 gatcaaagta ccaagaatac caataataat cacgtttcca aggcatgccc atgcaccctg
     3781 tgcaaatacc ttgttggcag gctctgcgta gatcacaatg tcaagtaccg gcgcaacgat
     3841 cacccatgca gcgaggttgg caacaacctg cacaacgttg aacagcacga tctgtttctt
     3901 ctcgaatccg ccgtccttga tcgcatattt tgcagcgaaa agtccgacca gaataccgaa
     3961 taccgcatcc gggaataccc agctccacca tacgcttcca taaaacagtg catcaccgat
     4021 tgcatgtccc agaagaccga caataccgcc gactaccgga ccgaatacgg ctgcaaggaa
     4081 tgcgagcaat gccg
//
LOCUS       DBMDQJ010000141         4094 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8141_length_4094_cov_1.605810, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000141 DBMDQJ010000000
VERSION     DBMDQJ010000141.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8141_length_4094_cov_1.605810"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="ACZO9R_04015"
     CDS             <1..341
                     /locus_tag="ACZO9R_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912268.1"
                     /translation="GGIGVMNIMLVSVTERTQEIGLKKAIGARKSRILGQFLTEAAVL
                     TSLGGLIGVLVGIILAQVISYVTTTPVAISVPAAVGAVAFSMVIGIVFGVFPSYKAAN
                     LNPIDALRHE"
     gene            383..1645
                     /locus_tag="ACZO9R_04020"
     CDS             383..1645
                     /locus_tag="ACZO9R_04020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912269.1"
                     /translation="MIKRIVMMEGGVETLSYFSHQMAGEFQKLGYAVFFYDLKQEESS
                     AGKLRKFIRPRETVLVTFNFQGLEKEAGVYREGIGYLWDTYHIPCYNIAADHPYFYDD
                     RLKDLPEKYRHISIDRRQKAYFEEFYPEYVSRGFLPLAGTGLRQGEDEAKTGKAGTQG
                     TAVETEEAGAQGDAEQAAPCYDVILTGNYTKLSFFEPYINWINEEYAAFYRGIIDDLL
                     EHPACTVEEVALAHCEREMGKEPNDQLRIALHKMIFIDLYVRNYWRGKAVRTLVNAGI
                     PVHVVGKGWEELEDVRHPECLKLHPQTDSVTCLGMLADAKVSLNVMPWFKDGAHDRVF
                     NSILNGAVCVTDPSRYLEEELHEGEGVCYVALQDMDALPEKVKDLLQNDSGRNEIVRR
                     GRAIVEQKHTWAQRAKTLAAWIAEDAAQ"
     gene            2069..3358
                     /gene="serS"
                     /locus_tag="ACZO9R_04025"
     CDS             2069..3358
                     /gene="serS"
                     /locus_tag="ACZO9R_04025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR0912270.1"
                     /translation="MIDLKFLRENPEVVKQNIKNKFQDHKLPLVDEVIELDAEARKTQ
                     QEADDLRAKRNQLSKEIGKLMGQGKKDEAEAVKTQVAEGAARLAELEEKEKELQEKVT
                     KIMMTIPNIIDPSVPIGKDDSCNVEIEKFGEPVVPDFEIPYHTEIMERFNGIDLDAAG
                     KVAGNGFYYLMGDIARVHSAVLSYARDFMIDRGFTYVVPPFMIRSNVVTGVMSFDEMD
                     AMMYKIEGEDLYLIGTSEHSMIGKFIDTINDEEKLPYTLTSYSPCFRKEKGAHGIEER
                     GVYRIHQFEKQEMIVVCKPEESKMWYDKLWQNTVDLFRSLDIPVRTLECCSGDLADLK
                     VKSCDVEAWSPRQKKYFEVGSCSNLGDAQARRLKIRVKGKDGSKYFAHTLNNTVVAPP
                     RMLIAFLENNLNADGSVNVPVALRPYVGGKEKLIPVK"
     gene            complement(3987..>4094)
                     /locus_tag="ACZO9R_04030"
     CDS             complement(3987..>4094)
                     /locus_tag="ACZO9R_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR0912271.1"
                     /translation="AETATDYSDRPLAPQVMKKVTVETFGVEYPEPEKC"
BASE COUNT         1064 a          868 c         1229 g          933 t
ORIGIN      
        1 tcggcggtat cggcgtcatg aacatcatgc tggtatcggt gacggagcgg acgcaggaga
       61 tcggtctgaa aaaggcgatc ggggcacgca agtcccggat cctgggacag tttctgacgg
      121 aggcggcggt gctcaccagc ctgggcggtc tcatcggcgt gctggtcggc attattctgg
      181 cgcaggtgat ctcgtatgtg acgacgacgc cggtggcgat cagtgttccg gcagccgtgg
      241 gagcggtggc gttttccatg gttatcggaa tcgtattcgg tgtgttcccg tcctacaagg
      301 cggcgaattt aaatccgatc gatgcgctgc ggcatgagta agcaaaaaat aatgacagcg
      361 ctgcggacag aaaggagctg gcatgataaa aagaattgtc atgatggagg gcggagtgga
      421 gacactatcc tacttctccc atcagatggc aggagagttt caaaaactgg gatacgcagt
      481 atttttctat gatctaaaac aggaggagag cagcgctgga aagctgcgca agtttatcag
      541 accgcgcgag acggtgcttg tgaccttcaa ttttcaggga ctggaaaagg aggccggcgt
      601 gtaccgcgag gggatcggtt atctctggga tacctatcac attccctgtt ataatattgc
      661 cgccgaccat ccgtactttt acgatgacag gttaaaagat ctgccggaaa aataccggca
      721 tatcagcatc gaccggagac agaaggcata ttttgaagaa ttttatccgg agtatgtgtc
      781 gcggggattt ctgccgcttg cgggaaccgg attgcggcag ggagaagacg aagctaagac
      841 gggaaaagcg gggacacagg gcaccgcagt tgagacggaa gaagcggggg cacagggtga
      901 cgcagagcag gcggcaccct gctatgacgt gatcctgacc ggaaattaca caaaactttc
      961 cttttttgaa ccctatatca actggatcaa tgaggagtat gcggcatttt accgggggat
     1021 catagacgat ctgttggaac atccggcctg cacggtggag gaagtggcgc ttgcccactg
     1081 cgagcgggag atgggaaaag agcccaatga tcagctgcgg attgccctgc ataagatgat
     1141 tttcattgat ctgtatgtgc gcaattactg gcggggaaag gcggtgcgca cgctcgtcaa
     1201 tgcgggaatt ccggtacatg tggtcggaaa aggctgggag gaactggagg atgtgagaca
     1261 tccggagtgc ttaaagctgc atccgcagac ggattccgtt acctgccttg ggatgctggc
     1321 ggatgcgaag gtgtctttga atgtgatgcc gtggtttaag gacggagcgc atgacagagt
     1381 gtttaattcc atcttaaatg gagcggtatg tgtgaccgac ccgagccgct atctggaaga
     1441 ggaacttcat gagggtgagg gggtctgcta tgttgcactg caggatatgg acgctcttcc
     1501 ggaaaaagtg aaggatttat tgcaaaatga ttccgggagg aatgagattg tgcggcgcgg
     1561 cagagcgatt gtggagcaga agcatacttg ggcacagcgc gcgaagacgc tggcagcgtg
     1621 gattgcggag gacgccgcgc aatagataaa ggacaaagtg cggaaaatac aggggattcg
     1681 atgcggactg ggcgaggatc gggcgaatct gcatattaac cttgcatttt gcggataatc
     1741 attatataat agcggaagaa ttggcgatga agaaagctgc ggtttgaaga ctgcgacaga
     1801 gagccggtgg gtggtgcgaa ccggtgcagg atagaatctt tccacttttg gagccggcag
     1861 tgaaagctgc gagggtcgta tcctttaaca tacgtttgag aggtcgcagg aacatgcctg
     1921 tggcaagatg ggtggcaacg cggaagcaga acctttcgtc ccatgtgcaa gtggtgcccg
     1981 gtcagagatg gcggggacat ttgacacagg ggcgaaaggt ttttttgttc ctgtgcggat
     2041 agaacagaag aatttaggag gaaatgacat gatcgatctg aagtttttga gagagaaccc
     2101 ggaggttgta aagcagaaca ttaagaacaa attccaggat cacaagcttc cgcttgtgga
     2161 cgaggtaatt gaactcgatg ccgaggcgcg caagacacag caggaagcag acgatcttcg
     2221 agcaaagaga aaccagctgt ccaaagagat cggtaaactg atgggtcagg gcaaaaagga
     2281 cgaggctgag gctgtcaaga cacaggttgc agagggagct gcccgtctgg cagaattaga
     2341 ggaaaaagaa aaggaacttc aggagaaagt caccaagatc atgatgacga tcccgaacat
     2401 cattgatccg tccgtaccga tcggcaagga tgattcctgc aatgtggaga tcgagaagtt
     2461 tggcgagccg gttgttccgg attttgagat cccgtatcac accgagatca tggagcgttt
     2521 taacggcatt gatctggatg ctgcaggcaa ggtggcagga aacggatttt attatctgat
     2581 gggcgatatt gcgcgtgtac actccgcagt gctctcctac gcgagagatt tcatgatcga
     2641 ccgcggtttt acctacgtag tgccgccgtt tatgatccgc agcaatgttg tgacgggcgt
     2701 tatgagtttt gatgagatgg atgccatgat gtacaagatc gagggcgagg acctttatct
     2761 gattggtacc tccgagcatt ccatgatcgg taagttcatc gatacaatca acgatgagga
     2821 gaagctgcca tatacactga catcctactc tccatgcttc cgcaaggaga aaggcgcaca
     2881 cggcatcgag gagcgcggtg tttatcgtat tcaccagttt gagaagcagg agatgatcgt
     2941 agtctgcaag ccggaagagt ccaagatgtg gtacgataaa ctgtggcaga acacggttga
     3001 cctgttcaga agtctggata tcccggttcg taccctggag tgctgttccg gcgatctggc
     3061 agatctgaaa gtgaagtcct gcgatgttga ggcatggtct ccgagacaga agaagtattt
     3121 cgaggttgga agctgctcaa acctcggcga tgcacaggcg agaagattaa agatccgtgt
     3181 gaagggcaag gatggcagca agtattttgc acataccttg aataacacgg tggttgcgcc
     3241 gcctagaatg ctgatcgcgt tcttagagaa caacctcaac gcagacggaa gcgtcaatgt
     3301 tccggtagca ctgcgtccgt atgtcggagg caaggagaag ctgattccgg taaaataaat
     3361 atgcacaaaa aacggtctgc cggatagggc agaccgtgct ggagtaagtg ttattatatg
     3421 gaggtaaaag cactgacagg ttctggaatg aaaataagcc tgaaccattc ggtggagaaa
     3481 aatgtgtgga tttcttccag acagattttg catctgattc cgtgtctctt ccacgtttgg
     3541 acacctggtg ggcgcatgtc cgacacaccg gagacaatgg gcggactttc tggcagcact
     3601 aactgtgctc cctcatagac attattaaac cgcgcgaaca gaactcgctg ctatctgcta
     3661 ttgaaattgt ggcagatagc agctcagaca tctgcttcgc gcggttatgt tttttcggga
     3721 gcacaggtaa gtgctgcaca aaagtcctac aaattgtctc acggagtgcg gacatgaccc
     3781 accgtggagt gctccaaact tggaaagaca cggatcatcg cagatgcaaa aatgctggtt
     3841 agaaatcctg cattttttga ttccgaatgt tcaagctaat tttcattcag aaccgtaatg
     3901 tgcctgtctc tacaataata acgtttcttc cagcacagac tgcgttagtt ggcagaccgt
     3961 tttctttagc atttcggaga attgcattag cacttctctg gttccggata ctccacaccg
     4021 aacgtctcga cagtcacttt tttcatgacc tgcggagcga gcgggcggtc gctatagtcg
     4081 gttgcagtct ctgc
//
LOCUS       DBMDQJ010000142         4066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8220_length_4066_cov_1.644136, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000142 DBMDQJ010000000
VERSION     DBMDQJ010000142.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8220_length_4066_cov_1.644136"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..139)
                     /locus_tag="ACZO9R_04035"
     CDS             complement(<1..139)
                     /locus_tag="ACZO9R_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015534220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HIR0912272.1"
                     /translation="MKNILLIGLGRFGKHIALQLNKLGHEVMAVDSNEERINEILPIV
                     TN"
     gene            complement(153..1490)
                     /locus_tag="ACZO9R_04040"
     CDS             complement(153..1490)
                     /locus_tag="ACZO9R_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR0912273.1"
                     /translation="MLEIIHRKKRLSSFKLIALGFAGVIVLGALILMLPFSSNTGSVT
                     PFHEALFTSTSAVCVTGLVVQDTGSYWSAFGQAVILLLIQIGGLGVVTVAATFAMLSG
                     RKISLMQRSTMQDAISAPKVGGIVRLTRFIVRGTFLIELIGMFAMLPVFCRDHGFKGI
                     WMAAFHSVSAFCNAGFDILGTTENKYPSLTGYVGEPTINIVIMILIIVGGIGFLTWDD
                     ICTNRWHFKKYRMQSKVILVTSVILIIVPAIFFFLFDFTGLPPGEKILASLFQSVTPR
                     TAGFNTADLTAMTESSKAIMILLMLVGGSPGSTAGGIKTTTLAVLILNAFSVCRKKND
                     TEVYGRRLECSAVKSASTIAVIYFLLFFIGGIIISTAESMPLSACLYETASAVGTVGL
                     TLGVTPQLSVFSQFILIVLMYLGRVGGLTLIYAAISNKNQSGAKLPLEKITVG"
     assembly_gap    1553..1562
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2029..2529
                     /locus_tag="ACZO9R_04045"
     CDS             2029..2529
                     /locus_tag="ACZO9R_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR0912274.1"
                     /translation="MFEIGEYIVYGVKGVCRIEDITHIDISGADKDRLYYVLAPVGDG
                     SGRIYAPTDNQKITMRKVISREEADQLIEDMPKIEQLWVPDDRQREARYKEAMNTCDY
                     RAWVSIVKTLYIRKQERTAQGKKITALDERYMRTAENELYSELSLTLGIPKDEMEQYI
                     RERLTQ"
     gene            complement(2706..3968)
                     /locus_tag="ACZO9R_04050"
     CDS             complement(2706..3968)
                     /locus_tag="ACZO9R_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4317 domain-containing protein"
                     /protein_id="HIR0912275.1"
                     /translation="MNKKDILELKRRFKKDACTFTRLCGCYVDADHNKVTTFGETFLN
                     LEDEEFYKYLEIAKKVMSGTIGNNILELEFPTAEEAPGGRQQFLMGLRESALKNDDLM
                     DTFYDLVIENYSYVGNYLILVFHDAYDVMTKTSDNNKLDESEEVYEYLLCAICPVTLT
                     KPGLGYREDENRIGPRIRDWVVGVPDTGFVFPAFTDRSSDIHSVMFYTRDTKTPHAEF
                     MESGLGCGTKLTATEKKLTFQSIVKEVIGEDEEESDAVFGDIQENLNNMIPVTEDETV
                     EPEPVIVTRSAIADVLAESGVTEEQAAVIENTYENVFGEELPEAGQLVDPKLVEANGK
                     RKEKLELVKQVENLKQQLEETRSVPVSEGDADGEDVPAVKTYDVILRVKPEKVGQIHS
                     QVIDGQKCLVIPMDEDEHAAVNGVNTTV"
BASE COUNT         1093 a          934 c         1000 g         1029 t
ORIGIN      
        1 cattggtgac gatcggcagg atttcattta ttctttcttc gttgctgtct actgccatca
       61 cctcatgccc gagtttgttc agttgcaaag caatatgctt accgaatctg ccgagaccta
      121 tcaataatat gtttttcata acagtaactc ctttatccga ctgtgatctt ttcgagcggc
      181 agttttgcac cgctctgatt tttattagag atcgccgcat agatcagggt aagtccaccg
      241 actcttccca gatacattaa gacgatcaaa ataaactgag aaaaaacact gagctgcggc
      301 gttacgccga gtgtaaggcc gaccgttccg acggcggatg ccgtttcgta aaggcaggcg
      361 ctaagcggca tactttctgc cgtgctgata ataatgccgc cgataaaaaa taacaggaaa
      421 tatattacgg cgatggttga ggcgcttttt accgccgagc attcaagccg tctgccatag
      481 acttcagtat cgtttttttt acggcagacg gaaaatgcgt tcaagatcag aacggcaagc
      541 gtagtcgttt ttataccgcc ggcggtggaa ccgggtgagc cgccgacaag catcagcagg
      601 atcataattg ctttggaaga ttcagtcatg gcggtaaggt cggcggtgtt gaatccggcg
      661 gttctcggtg tgacagactg aaaaagcgag gcaaggatct tctcaccggg cggcagtcct
      721 gtgaaatcga agaggaaaaa aaatattgcg gggacgataa taagaatcac cgatgttact
      781 aatattacct tgctttgcat acggtatttt ttgaaatgcc acctgtttgt acagatatca
      841 tcccaagtca aaaagccaat gccgcccacg ataattaaaa tcataattac aatgtttatt
      901 gtcggctctc cgacgtatcc cgtaagagat ggatatttgt tttcggtggt gccgagtatg
      961 tcgaatcctg cattgcaaaa agcggagacc gaatgaaacg ccgccatcca aattcctttg
     1021 aaaccgtgat ctctgcaaaa taccggtagc atggcaaaca ttccgataag ttcgatcaga
     1081 aaagtcccac gtacaataaa gcgagtcagg cgaacgatgc cgccgacttt tggggctgaa
     1141 atggcatcct gcatggtgct gcgttgcatc agcgatattt ttctgccgga aagcatggca
     1201 aatgttgcag caaccgtcac aacgccaagt ccgcctatct gaataagcag caaaatcacc
     1261 gcctgcccga aagcggacca atagctgcct gtatcctgca caacaagccc cgtaacgcaa
     1321 acagcggagg ttgaagtgaa cagtgcctca tgaaatggtg taacagatcc ggtgtttgat
     1381 gaaaatggta gcatcaagat aagtgctcca agtacaatga ctccggcaaa acccagagca
     1441 atcagcttaa aggatgacag ccgttttttt ctatgtataa tttcaagcat atatatttct
     1501 cctttttctg cttgcttaca aagagtataa cgtgaaatat aaaaaaaagg tgnnnnnnnn
     1561 nnactgtatt aagatcgtat aaagatgccc atgctaaaaa aatagtgcag cgtatattaa
     1621 acggcacaga caaagacaaa ttgcttgaaa ctgttgtgca gctcgaaaca gaacaacagt
     1681 ttcaagcaat ttgaaaaagc ggatgcagag attttggaca gacgtgaaca atacaccgcc
     1741 tcctactcga ttgggagaat tcatccctgt cacacaaaaa tacgttgtga agatgcgtga
     1801 ccgcgcgaat gggaagcatg ccgcatgacc gcgcccggcg caaaaattct gtgacaggag
     1861 tcttttttac ttcctatttt ggcaaatatg tacaatcata cgccgcctgt tttctggcaa
     1921 tagttacgaa aattggaaaa aagcggaaaa tattgattaa atcgggattt ttcgtgttaa
     1981 aatagaaatg gatgctttaa aactgtgatg aaaggagaat cggggaagat gtttgagatc
     2041 ggtgagtata ttgtgtatgg agtgaagggt gtatgccgga ttgaggatat tacccacatt
     2101 gacatttccg gggcggacaa agaccgtttg tattatgttc ttgccccggt tggcgatggc
     2161 agtggaagga tctatgcacc gacggacaat cagaagatta cgatgcgcaa agtgatttcc
     2221 agggaggaag cggatcagct gattgaggat atgcctaaga tcgagcagct ctgggtgccg
     2281 gatgacaggc agagagaggc gagatacaag gaggcaatga acacctgcga ttatcgtgcg
     2341 tgggtcagca ttgtaaagac actatacatc agaaaacagg agcgcacggc acaggggaag
     2401 aagatcacgg cgctggacga gcgctacatg cggacagcgg agaatgaact gtacagtgaa
     2461 ctctcgctca cactcggcat accgaaggat gagatggagc agtatatcag agagcggctg
     2521 acgcaataaa cgctgtgcct gcgtctgaaa caggattgtt atgaaaaacc cgtcatgaaa
     2581 tgagaaagga aatcatttca tgacgggttt tatgcaaaat aggatacggt ggcatgaatg
     2641 ccgtcactgc tggcttctgc cggtatggaa catattccat acgtcatcga ttgcatcgat
     2701 ccgtattata cggtggtatt gacaccgttg acagcggcgt gctcatcttc atccatcgga
     2761 atgaccagac atttctgtcc gtcgatgacc tgggagtgaa tctgtccgac tttttccggc
     2821 ttgacgcgca ggatgacatc gtaggtcttg acggcaggga catcttcgcc atcggcatct
     2881 ccctccgaga ccggaacgga gcgcgtctcc tcaagctgct gctttaagtt ttcgacctgt
     2941 tttaccagct ccagcttttc tttgcgcttt ccgttggctt ccacaagctt cgggtcaact
     3001 aactgtccgg cttccggaag ctcctcgcca aacacatttt cataggtgtt ctcaatgacg
     3061 gctgcctgtt cctcggtgac accgctttct gcaaggacat ctgcaatggc ggatctggtg
     3121 acgatgaccg gctccggttc caccgtctcg tcctccgtga ccggaatcat attgttcaga
     3181 ttttcctgga tatccccgaa gacagcgtcg ctctcttcct catcctcacc gatgacttct
     3241 ttgacgatgc tctggaatgt cagctttttc tcggtggcgg tcagcttggt gccgcagccg
     3301 agaccggatt ccatgaattc cgcgtgcggc gtcttggtat ccctggtgta gaacatcacg
     3361 gagtgaatat cgctgcttcg gtcagtaaat gccggaaaaa cgaaaccggt gtccggaacg
     3421 ccgaccaccc agtcacggat gcgcggaccg atacggttct cgtcctcgcg gtagccaagt
     3481 cccggcttgg tcagggtaac cggacagatg gcgcacagca gatactcgta gacttcctcg
     3541 gactcgtcca gcttattgtt gtcggaggtc ttcgtcatca catcataggc atcatggaac
     3601 accagaatca gataattccc gacataactg taattctcaa tgacaagatc atagaaggtg
     3661 tccatgaggt catcgttttt taatgcgctc tcgcgcagac ccatcaaaaa ctgctgtctt
     3721 ccgcccggtg cttcctcggc agtcggaaat tccagttcca gaatgttgtt gccgatcgtg
     3781 ccggacataa cctttttggc aatttccagg tatttataga attcctcatc ctccagattt
     3841 aagaaggtct ccccgaaggt tgtgaccttg ttgtggtctg cgtcgacgta gcagccgcag
     3901 agtctggtga aggtgcaggc atctttttta aaacgtcttt ttaactccaa gatatctttt
     3961 ttattcatat atcatcatcc tatgcctttc gtaaatcgtg cacaagatat tatagcgaaa
     4021 aaatgggtgg tgtgcaagaa gaatggaatg tggacacggg agaaat
//
LOCUS       DBMDQJ010000143         4046 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8272_length_4046_cov_1.377947, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000143 DBMDQJ010000000
VERSION     DBMDQJ010000143.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4046)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4046)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4046
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8272_length_4046_cov_1.377947"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1022)
                     /locus_tag="ACZO9R_04055"
     CDS             complement(<1..1022)
                     /locus_tag="ACZO9R_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR0912276.1"
                     /translation="MNIQKFTQKSVEAINDCEKLAYEYGNQEIEQEHLLVALMRQEDG
                     LIPKLIEKMEIQKEHFMDNAERHLAARVKVSGGQVYVGQDLNKVLIHAEDEAKQMGDE
                     YVSVEHLFLALIKYPNKALKEIFKEYGITRERFLQALSTVRGNQRVTSDNPEATYDTL
                     EKYGYDMVARARDQKLDPVIGRDDEIRNVVRILSRKTKNNPVLIGEPGVGKTAVVEGL
                     AQRIVKGDVPEGLKDKKLFALDMGALIAGAKYRGEFEERLKAVLDEVKSSEGQIILFI
                     DELHTIVGAGKTDGAMDAGQLLKPMLARGELHCIGATTLDEYREYIEKDAALERRFQP
                     VMVYEPT"
     gene            complement(1195..>1634)
                     /locus_tag="ACZO9R_04060"
     CDS             complement(1195..>1634)
                     /locus_tag="ACZO9R_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0912277.1"
                     /translation="GFAEVLQQNENWVMLERQSGDFTQAKGQEVMKEFLEKYEDIDVV
                     ISENDNMTFGAIEAIREAGRTIGSDGDIIILSFDAVRDALKAIQTGDIAADFECNPLT
                     APLVEETIRRLEAGESVEKIQYVEETYFDCEMDLSELLESRPY"
     assembly_gap    1635..1644
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1645..2227)
                     /locus_tag="ACZO9R_04065"
     CDS             complement(<1645..2227)
                     /locus_tag="ACZO9R_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002584275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0912278.1"
                     /translation="MGKKGIAAALLASQLLFTAGCGSGEQRETAGQLDTEQTMQQDGY
                     IVVGYVQVGSESDWRTTNTQSFKDVFTQENGYYLIFEDGQQKQENQVKAIRNLILQEV
                     DYIVLDPIVETGWESVLEEAKEAGIPVILSDRRAEMEDDSLYTCWVGSDFAEEGRNAG
                     HWLENYLEKQGRSGETVHIVSLQGTLGSSAQIGR"
     gene            complement(2383..3399)
                     /locus_tag="ACZO9R_04070"
     CDS             complement(2383..3399)
                     /locus_tag="ACZO9R_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR0912279.1"
                     /translation="MKAKKLNGNTVLLIITIALFFVMYAVGCVIYSNKGFTHLQTFLN
                     ILINNAGLLCITCGMTCVMLTGGIDISVGSLVAMDCMILAVGMERWNMNAVLLCILVL
                     AIGIVFGVVQGFCVGYLNIQPFIVTMAGMFFARGMTAVISTEQISISSNAFFVKLANT
                     KISLPFGSYTNNKGVVQVPFIRVSVIVALLVLIAVFFMLRYTKFGRSLYAVGGSEQSA
                     TMMGLDVKKTKLKAYVLSSFLCSIGGICYCLNTMSGNVQQALGLEMDAIASSVIGGTL
                     LTGGVGNVIGSFFGVLINGTISTLVKTNGKLLSSWSNIAVAALLCFFIVLQSIFAKIK
                     EKSK"
     gene            complement(3399..4022)
                     /locus_tag="ACZO9R_04075"
                     /pseudo
     CDS             complement(3399..4022)
                     /locus_tag="ACZO9R_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856928.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT          924 a         1230 c          904 g          978 t
ORIGIN      
        1 gtcggctcat acaccattac cggctggaaa cgacgctcga gcgccgcatc tttttcaatg
       61 tattcgcggt actcatccag ggtcgtcgca ccgatacaat gcagttcacc acgcgcaagc
      121 atcggcttta acagctgtcc ggcatccatc gcaccatccg tcttgcccgc acccacgatc
      181 gtgtgaagct catcaatgaa caggataatc tgtccctcgg aacttttcac ctcatcaagc
      241 actgccttta accgctcctc gaattcaccg cgatattttg caccggcgat cagcgcaccc
      301 atatccagtg caaacaattt cttgtccttt aagccctccg gcacatcgcc ttttacaatt
      361 cgctgggcaa gcccctcgac aacagccgtc ttgccgacac ccggctcacc gatcagcacc
      421 gggttgtttt tcgtcttacg ggacagaatc cgcacgacat tccggatctc atcatcacgt
      481 ccgatgaccg gatccagctt ctggtctctc gcacgggcta ccatatcata cccgtacttc
      541 tccagcgtgt cgtaggttgc ttccggattg tccgatgtca cgcgctgatt gccgcggact
      601 gtcgagagcg cctgcaaaaa ccgttctctg gtaatgccgt actccttaaa gatctccttc
      661 aatgccttat tcggatactt gatcagcgcc aggaacagat gctcgacaga aacatactcg
      721 tcccccatct gcttcgcctc atcctcggca tgaatgagca ccttatttaa atcctgtccg
      781 acatagacct gaccgccgga caccttgaca cgcgctgcca gatgccgctc ggcgttatcc
      841 atgaagtgct ccttctgaat ctccatcttc tcaatcagct tcggaatcag tccatcctcc
      901 tgccgcatga gtgcaaccag aagatgctcc tgctcgatct cctgatttcc atattcatag
      961 gcaagcttct cgcaatcgtt aatggcttcc actgactttt gtgtaaattt ctgaatgttc
     1021 ataagcgtac ctctctttct cttcctatga tatacaggcg gcacatatct atgcgccgcc
     1081 tgctttgttc tccctgttac aattatgtta tagcaccagt tgttagcact gtcaagaggt
     1141 gagtgctaat tttttcagat aattttaccg ccagcagaag cagggacttc ctgcctaata
     1201 tggtctgctc tccaacagct ccgagagatc catctcacag tcaaaatatg tctcctccac
     1261 atactggatc ttctccacgc tttcccccgc ctccaggcgg cggatcgtct cctccacaag
     1321 cggggctgtc agcggattgc attcaaaatc agccgcgatg tccccggtct ggattgcctt
     1381 gagggcatcc cttaccgcat caaaggacag gataataatg tccccgtcac tgccaatcgt
     1441 cctgcccgcc tcccggatcg cctcgatagc gccaaaggtc atattgtcat tctcgctgat
     1501 tacaacatca atatcctcgt atttttcgag aaattccttc ataacctcct gcccctttgc
     1561 ctgcgtgaaa tctcccgact ggcgctccag catcacccag ttttcattct gctggagaac
     1621 ctctgcgaat ccgtnnnnnn nnnntccgcc cgatctgtgc agaggagccc agcgtcccct
     1681 ggagagatac gatatgaact gtctccccgc tccgcccctg cttttccaga taattctcca
     1741 gccagtgccc ggcgttcctg ccctcctccg cgaagtcact gcccacccag catgtataca
     1801 ggctgtcgtc ctccatctcc gccctgcggt cagacagaat caccgggatt ccggcttcct
     1861 tcgcctcctc cagaacggat tcccagcctg tctccacgat cgggtcgagc acgatataat
     1921 ccacctcctg caggatcaga ttgcggattg ccttcacctg gttctcctgc ttctgctgtc
     1981 cgtcctcgaa gatcagatag tagccgtttt cctgcgtgaa cacatcctta aaggactgtg
     2041 tgttggtggt tctccagtcg gattccgagc cgacctgcac atatccgacc acaatatagc
     2101 cgtcctgctg catcgtctgc tccgtatcca gctgtcccgc agtctctctc tgctccccac
     2161 tcccacagcc ggctgtaaag agcagctggc tggcaagcag cgccgccgcg attccttttt
     2221 ttcccacagt cctcttctga aatatgttca tattcctccc gccttttctc ctgccggctg
     2281 cacctcctgc ccggggtgct tttttaacgc agcacggtat tttcccatag aggagccaaa
     2341 aggctgccgc atgaagtgga cagcccctgg acagccttgt ttttatttgc tcttctcttt
     2401 gatctttgca aagatactct gtaatacgat gaagaagcag agcagcgcag ccaccgcaat
     2461 gttggaccac gagctgagca gcttgccgtt cgtcttcacc agtgtggaaa tcgttccgtt
     2521 gataagtaca ccaaagaagg agccgatgac atttcccaca ccgcctgtca ataatgtacc
     2581 gccgataacg gaggaagcaa tggcatccat ctcaagtccg agtgcctgct gcacatttcc
     2641 cgacatcgtg ttcaggcagt agcagatgcc gccgatcgag cagaggaatg aggaaagcac
     2701 atacgccttc agcttcgtct tcttcacgtc cagtcccatc atggtcgccg actgctcact
     2761 tcctccgact gcgtaaaggc ttctgccgaa cttggtatag cggagcatga aaaatacggc
     2821 tatcagcacg agcagcgcca cgattaccga tacacggata aacggaacct gcaccacgcc
     2881 cttgttgttg gtataggagc caaacggaag gctgatcttc gtattggcaa gctttacaaa
     2941 aaatgcattg ctgctgatgg agatctgttc cgtgctgatc acggctgtca tgcctctggc
     3001 gaagaacatt cccgccattg tcacaatgaa cggctggata ttcagatagc cgacacagaa
     3061 gccctgcacc acgccgaaca caatcccgat ggcaagcaca agtatgcaga gcagaaccgc
     3121 attcatgttc cagcgctcca tgccgacggc aagaatcatg cagtccatgg caaccagcga
     3181 gccaacggaa atatcgatgc cgccggtcag catcacgcag gtcattccac aggtgatgca
     3241 gagcagaccc gcattattga taaggatgtt caaaaacgtc tgcagatgtg tgaagccctt
     3301 attggaataa atcacacagc ccacggcata catcacaaaa aatagcgcga tggtaattat
     3361 cagaagaacc gtatttccat ttaatttttt cgctttcatt atgcagcacc tcctttggca
     3421 agctgtctcc ttacagacat ttttttcata aatgccttaa acggaggtgc ctggattgcc
     3481 acaataatga taacaatgac cgccttgtac accggtgcct gctcggtcga gatgccgagc
     3541 gcatacaggg tggtggtgat cgcctggatg gtgtacgcac cgataataga gcctgccagc
     3601 cggaactttc cgccgccgag gctgttgccg ccgagtgcaa ctgccagaat cgcatccagc
     3661 tcgaagttca gtccgatgtt gttggaatcc gcagagctga tccgggagga agcaacaata
     3721 ccggcaattc ctgcacacaa tccgcagaca acatagcaca ggaaaatgat gcgctcggaa
     3781 ttgatgcccg cgatgcggga agccttctta ttgatgccga cgctctggat atataagccc
     3841 agtgcggtct tgcggagcac cagcgatgtg atcaggatgc acacgatggc aaccacgatc
     3901 ggtgtcggca taaatccgag atagccgccg aagattttga aggaatcatc ccggacgtat
     3961 acgatcatgt tgttgcagag cagaagtccg attgccctcg ccgccgacca acggatcgag
     4021 gtgcaaaaac ttcgccaggc ctaccg
//
LOCUS       DBMDQJ010000144         4045 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8276_length_4045_cov_1.313641, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000144 DBMDQJ010000000
VERSION     DBMDQJ010000144.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8276_length_4045_cov_1.313641"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(1..606)
                     /locus_tag="ACZO9R_04080"
                     /pseudo
     CDS             complement(1..606)
                     /locus_tag="ACZO9R_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009204676.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
     assembly_gap    607..616
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    1004..1013
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1114..1239
                     /locus_tag="ACZO9R_04085"
     CDS             1114..1239
                     /locus_tag="ACZO9R_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912280.1"
                     /translation="MEERITDTSLIVENRNTCDDNSPTVPLTEKQIAELRACAAK"
     gene            1352..1930
                     /locus_tag="ACZO9R_04090"
     CDS             1352..1930
                     /locus_tag="ACZO9R_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HIR0912281.1"
                     /translation="MLYYREISLKDQRKCLLKNAERADAAAFLHYFTQAHAETDFLTT
                     YADEGTHSVEDMKERLAAQKESRTEIEICAFVDGVLVGSAGNHALSTREKLRHRADFG
                     ISILREYWGQGIGAALTGACIDCAKEAGFRQLELEVVGENETAVRLYKKYGFMEYGRN
                     PRGFLTRDGRWQELVLMRLELEDAGARKNNAV"
     gene            1984..2274
                     /locus_tag="ACZO9R_04095"
     CDS             1984..2274
                     /locus_tag="ACZO9R_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912282.1"
                     /translation="MRRKAVRSEVEDAKAQKMMRGMERAMFFKEDTTFNDIRDKSLQQ
                     WLDEMEQHEDVAVRGGVKLTREYVQYLKDEIGRLRHENEVKNAYLKKLREKK"
     gene            2287..2943
                     /locus_tag="ACZO9R_04100"
     CDS             2287..2943
                     /locus_tag="ACZO9R_04100"
                     /inference="COORDINATES: protein motif:HMM:NF042628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6548 family protein"
                     /protein_id="HIR0912283.1"
                     /translation="MPENTNIIFFDEYKKLDKLCSEMYGKNSGGVTCYLNDMMAVPVM
                     QRNRIPKWNQTYDRLRELRHIRNQMAHGEGSFEDYPCSEEDVLWLFEFRSKIMHISDP
                     LAVYRRQTEGSTHATRAKGEHQLAVDHWQTEESMHAARAKEDFLLAVDQSQTERNIAD
                     HQRIKMDNVEVIDQEENDSFEKEHSMLKEIAVFGLIVLIVAATVLIFGNVYMKYFLLS
                     "
     gene            2998..3927
                     /locus_tag="ACZO9R_04105"
     CDS             2998..3927
                     /locus_tag="ACZO9R_04105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HIR0912284.1"
                     /translation="MKYQSVAILGAGAVGSYVMWGLAKKKEIDLCVVASGARKARYER
                     DGFVINGQVYHPAVRTPEEAAGVDLFIVATKYQALRPALGDIRTVVDGHTTVMSLMNG
                     VDSEEIIGEVIDPSQIVHSLIKVASERKGNQVVFDPETTIGIVYGEADISRSTGRIEA
                     LNDLFADTGLHYRATDVILTEIWSKFRLNVPNNQAQAMVGCGVGAYRDSEHVAFLRDR
                     LREEVDRIAEKKGIDFDKVDTSSTFGSKVRDRARYSTLQDLDAGRHTEVDMFAGAVVR
                     MGRELGIPTPYNEFTYHMIKALEEKNDGKFDYE"
BASE COUNT         1141 a          748 c         1262 g          874 t
ORIGIN      
        1 ggacattgtt gcagaaaatg gataccagac tgttcgtgag ctgcatgata aatccggcgg
       61 tgccgaggct tataatgttt tttgcttcga ccagactgga tttccactca gccgggcgca
      121 gcagccgcac cttatattcc gcgcgccggt gtagaaagcg gatcacatag agcgcggaca
      181 gcgtctggga gagaacggtg gcaagtgcgg caccggtgat tcccagatga aagacaaaaa
      241 taaaaagcgg atctaagaga aaattggtca cggcaccgat ggcaaccgag agcatgccgg
      301 cgacggagta cccctgcgcg ttgataaagg ggttcatacc ggtggcaatc atggacggca
      361 gcgtgccgag cagatagatc atcaggtaag gataggcgta tggcagggcg gcatccgagg
      421 cgccgaacag cttaagaatt ggcgctgcaa acagcagacc tgctgccatc agcaccagag
      481 caccggcgca tagcatggta aatgaggtat tcatgatctg accggctttt ttggtatctc
      541 tgccgccgcg gctgattgag aaaagcgggg cgccgccgct gccgaaaaga ttgctgaagg
      601 cggtgannnn nnnnnnggat gcgggcaata tagatccggt ctacaatatt gtagagcaga
      661 cttaagagct gtgcgaccag catgggaagg gcggctccca aaatatttcc ggtgatggtt
      721 ccatgttcaa aatcgattcg tttcatatga taaacttcct ttatgtatct gatgtatctg
      781 cgcggatttg ttgttgcttt acgctgcaat ccagtatacc atgatttaga acagatgaaa
      841 aatattgttt ggatgagacc tgatcagacg ggaagagagt ggaaagggag gatgggacgg
      901 ggaccggaaa agtcaccgtg tacccggtgt tttccgaaat tgaactgtac tacaatgaca
      961 tgcacgtggc gtactgcaac aaggagcaga agactgtgta tggnnnnnnn nnnttcttac
     1021 taataaaaat agaaaaacag ggcagttttt tgtggaggaa aaggagagag atatggatgt
     1081 ggagataaaa aagtgaatgc gattgtggct cgcatggaag aaaggataac agacacaagt
     1141 ttaattgtgg aaaacaggaa cacctgtgat gacaattctc cgacggtacc gctgacggag
     1201 aagcagattg cagaattacg tgcgtgtgca gcaaagtagg ttccgatgtg tccaggagtg
     1261 cgttctttct ttttgcgtag gagtgtgcta tactcggtga caaatgaggt ttgatgaaag
     1321 gcttgctgcg caaaagcggg agaggagcaa tatgttatat taccgggaga tcagcttaaa
     1381 agatcaaaga aaatgtcttt tgaaaaatgc ggaacgggcg gatgcggcgg cgtttctgca
     1441 ttattttaca caggcacacg cggagacaga ttttctgaca acgtatgcgg acgaagggac
     1501 gcactcggtg gaggacatga aagaacggct tgccgcgcaa aaggagagcc gcacggagat
     1561 tgagatctgt gcatttgtgg acggcgtcct tgtgggatcg gcgggaaacc atgcgcttag
     1621 cacgcgcgag aagctaaggc accgcgcgga tttcggcatc agtatcctgc gggaatactg
     1681 gggacaggga atcggtgcgg cactgaccgg cgcctgcatt gactgcgcga aagaagccgg
     1741 attccggcag ctggaacttg aggtggtcgg tgaaaatgag accgcagtga gactgtataa
     1801 gaagtatggg tttatggaat atggaagaaa tccgcggggc tttctgacac gcgacggcag
     1861 atggcaggaa ctcgtgctga tgcggttgga actggaagat gcgggtgcgc ggaaaaataa
     1921 tgcggtctga ggtggaagta cagatgcgcg ggaaagcagt gcggtctggg ctggaagatg
     1981 cggatgcgca gaaaagcggt gcggtctgag gtggaagatg caaaggcgca gaaaatgatg
     2041 cggggaatgg agagagcgat gttttttaag gaagatacga cgtttaatga tattcgggat
     2101 aaatcgctgc agcagtggtt ggatgagatg gagcagcatg aggacgtggc ggtccggggc
     2161 ggcgtaaagc tgacaagaga gtatgtgcag tatttgaagg atgagatcgg aagactccgg
     2221 catgagaatg aagtaaagaa tgcatatttg aaaaagctgc gggagaagaa gtaggaggta
     2281 cagaacgtgc cggagaatac aaatatcatt ttctttgatg aatataaaaa gctggataaa
     2341 ctttgttcgg aaatgtacgg taaaaacagc ggcggagtta catgttatct gaatgatatg
     2401 atggctgttc cggtcatgca gagaaacagg attccgaaat ggaatcagac atatgacagg
     2461 cttagagaac taagacatat aaggaatcag atggcacacg gggaaggttc gtttgaggat
     2521 tatccctgca gtgaagaaga tgttttatgg cttttcgagt tccggagtaa gatcatgcat
     2581 atctcggatc cactggctgt atatcgaaga cagacagagg gaagcacgca tgccacccgc
     2641 gcaaaaggag agcaccagct ggctgtagat cactggcaga cagaagaaag tatgcatgca
     2701 gcccgcgcaa aagaagattt cctgctggct gtagatcaaa gccagacgga gagaaatatt
     2761 gcagatcatc aaagaataaa aatggataac gtggaagtga tcgaccagga agaaaatgat
     2821 tcttttgaga aagaacatag catgttaaag gagattgccg ttttcggatt gattgtgctg
     2881 atcgttgcag ctaccgtgtt gatctttggc aatgtgtata tgaaatattt tctgctttca
     2941 taattagcgg agtgaggaaa taaataagcg ggtatcaaga gaaagagggt taaagcaatg
     3001 aaatatcagt cggttgccat tcttggcgca ggagcagtgg gttcttatgt catgtggggg
     3061 cttgcaaaaa agaaagagat tgatctgtgc gtagtcgcat cgggagcgcg gaaggcgcgc
     3121 tatgagaggg atggctttgt cattaacggg caggtatacc accccgctgt caggacgccg
     3181 gaggaggctg ctggtgtcga tcttttcatc gttgcaacca agtatcaggc acttcgcccg
     3241 gcacttggcg atattcggac tgtagtcgac ggacacacga cagtgatgag tctgatgaac
     3301 ggcgtggaca gcgaggagat catcggagag gtcattgatc cgtcccaaat tgtccattca
     3361 ttgatcaagg ttgcctcgga gcgaaagggc aatcaggtgg tattcgatcc ggagacgacc
     3421 atcggtattg tatatggaga ggcagatatt agcagaagca ccggacgcat tgaggcgctg
     3481 aatgatcttt tcgcggatac agggctgcac taccgtgcaa cagacgtcat cctcacggag
     3541 atctggagca aattccggct caacgtaccg aacaaccagg cacaggcgat ggtggggtgc
     3601 ggggtaggcg cctaccgtga cagtgagcat gtggcatttt tgcgggaccg gctgcgggaa
     3661 gaggtggacc ggatcgcgga gaaaaagggg atcgattttg acaaggtgga tacctcgtcc
     3721 actttcggca gtaaggtgcg ggatcgggcg cgctattcca cgctgcagga tctggatgcg
     3781 gggcggcaca cggaagtgga catgttcgcc ggcgcggtgg tgcgcatggg gcgtgaactg
     3841 gggatcccga caccgtacaa tgagtttact tatcatatga taaaggcact ggaggagaaa
     3901 aacgacggga agtttgatta tgagtagggg atggggaaaa ggtttgatat tagaaagtag
     3961 tcgtataagt gtttgaataa aaggagaagg aaactatgtt taaaacaaaa tcgggcagag
     4021 agatttcgtt tttgggagag gagaa
//
LOCUS       DBMDQJ010000145         4045 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8277_length_4045_cov_1.281694, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000145 DBMDQJ010000000
VERSION     DBMDQJ010000145.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4045)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4045)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4045
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8277_length_4045_cov_1.281694"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="ACZO9R_04110"
     CDS             <1..1314
                     /locus_tag="ACZO9R_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR0912285.1"
                     /translation="SNAVPTADYININVDATVNIKISNDPEKLRLAAENFLNKNTEYI
                     AGVAREVLEGNVREIVGKMKLEEMVSDRQKFANLVKENAEPDLAAMGLDIISFNVQNF
                     VDGNEVIENLGIDNIVKIKKSAAIARAESERDIKVAQAAADKESNDAAVAAQTEIAKK
                     QNELAIKKSELQMEADTKKAMADAAYEIQKEEQRKTIEVTTANADIAKQEREIELKQK
                     EVAVKEQSLEAEIKKQAEADKYAAQQRAEAELYQRQKDAEARQFEAQREAEARKAQAE
                     AERYAKEQEAAGIRAVGEAEASAIQAKGIAEAEAMEKKAEAYAKYNKAAVAEMMIKVL
                     PDIAAKVAEPLGQIDKITIIGGGEGGNGVDQVAGNVPVVMAKVFESMKEATGIDLADI
                     INAESYDAQVNRNINVSGLDSVNLVVKDEKKGAEQPDAQDGATIE"
     gene            1314..1940
                     /locus_tag="ACZO9R_04115"
     CDS             1314..1940
                     /locus_tag="ACZO9R_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912286.1"
                     /translation="MTVEVGVNRRAGTGQSVTAAVFIVMAAGSIAVIPLLAANLDRRA
                     LGIAACVLTLVFWVGFIGAICCVGEIVNTPTRAFLLTSDWQLYYVHFAARDYGPAPVT
                     KAGEIVHNYKVLSEEKKGRKWRREYLGSEEFRSMAQQYLEGVRTDTMGCVIEHLQTPS
                     IRSEGIDGSVLRYWDDARKKWATIRLLRTNTGYEKICRTVKLRQELGR"
     gene            complement(2565..>4045)
                     /locus_tag="ACZO9R_04120"
     CDS             complement(2565..>4045)
                     /locus_tag="ACZO9R_04120"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912287.1"
                     /translation="LSHPAVAAQSRNKTAIAGTCTMNTVLAAAYIAEVLKGVRSLPSY
                     LIFLLLCLLPCVTSILAYTKQKDTSSVRYLCGGGFALLYAYVMFTSSTDLTFCYVIVM
                     LLILLVYADKKLVSLLCTYALVINILVLVFRAVTQGFTAAQITNAEIIIACIILSYIF
                     VLMSVTKIAQINDANIKKADSDRTQSDDLLKLTLKVAGDMAGNIDQVSHSTSQLRDSI
                     AGTQTAMEDLTSGTSETAAAIQVQQQQTENIDQQVQNVSSVTAELLSSTAETSANLTA
                     SKDVMKNLVQQVQVSEASSTRVADSMEELKEYADKMQSIVALISSVASQTSLLALNAS
                     IEAARAGEAGRGFAVVAEEISSLAGQTNGATGDINQLIEHISQSISSVTDSVTELFDS
                     NRHQSEYITSAAGYFDLIEKNTASITSGMSELKQGVDAVSDSNSQIIQSIENVSALTQ
                     EVTASAGATLAGCRTNLQSVDDIALIMQQLQENANELQKNTQ"
BASE COUNT         1081 a          907 c         1193 g          864 t
ORIGIN      
        1 agcaatgccg tgccgacagc ggattacatc aatatcaatg tcgatgcgac cgtcaacatc
       61 aagatcagca acgatccgga gaagctgcgg cttgcggcag agaacttctt aaataaaaac
      121 accgagtata ttgccggggt ggcaagagag gttctcgagg gtaatgtgcg tgagatcgtc
      181 ggtaagatga agctcgagga gatggtatcc gaccgccaga agtttgccaa cctggtcaag
      241 gaaaatgcgg agccggatct tgcggcgatg ggactcgaca tcatcagctt taatgtccag
      301 aatttcgtgg acggcaatga ggtcattgaa aatctgggta ttgacaacat tgtaaagatc
      361 aagaagagtg cggcgattgc ccgtgccgag agtgagaggg acatcaaggt tgcacaggca
      421 gcggcggaca aagagtccaa tgatgcagcg gttgcggcgc agacggagat cgcgaagaag
      481 cagaatgagc ttgccatcaa gaagtccgaa cttcagatgg aggcggacac gaagaaggct
      541 atggccgatg cggcttacga gatccagaaa gaggagcagc gtaagacgat cgaggtgaca
      601 accgccaacg cggatattgc aaagcaggag cgtgagatcg agctgaagca gaaagaggtt
      661 gcagtcaagg agcagtctct ggaggcggag atcaagaaac aggcggaagc ggataagtat
      721 gcggcgcagc agcgcgcgga ggcagaactc taccagcgcc agaaggacgc ggaggcgaga
      781 cagttcgagg cacagcgcga ggcggaggcg agaaaagcgc aggcagaggc agagcgttac
      841 gccaaggaac aggaggctgc cggtatccgt gcggtcggtg aggcagaggc atctgcgatc
      901 caggccaaag gtattgcaga ggcggaagcg atggagaaga aagcggaggc gtacgcaaag
      961 tacaataaag cggcagtggc agagatgatg atcaaggtac ttccggacat cgcagccaaa
     1021 gtggcagagc cgctcggtca gatcgacaag atcacgatta tcggcggcgg agaaggtgga
     1081 aacggtgtgg atcaggttgc cggcaatgtg ccggttgtca tggcaaaagt atttgagagc
     1141 atgaaagagg ctaccggtat tgatctggca gacatcatca acgcagagtc ctacgatgca
     1201 caggtgaacc gcaacatcaa tgtgtcggga ctagattccg tgaatcttgt ggtaaaagat
     1261 gaaaagaaag gcgcagaaca gccggatgcg caggacgggg caacgataga gtgatgacgg
     1321 tggaagttgg cgtcaaccgc agagcgggaa cggggcagtc tgttacggcg gcagttttta
     1381 tcgtgatggc ggcaggatcg atagcggtga tcccgctgct tgcagcaaac cttgaccgta
     1441 gagcactcgg gattgcggcg tgtgttctca cattagtttt ctgggtcggg tttatcgggg
     1501 caatctgctg cgtgggggag attgtaaata cgccgaccag ggcgttcctt ctcaccagtg
     1561 actggcagtt gtattatgtt cattttgcag cccgcgacta cggacctgca ccggtgacaa
     1621 aagccgggga gatcgtacat aattacaagg tgctctccga ggagaaaaaa ggacggaaat
     1681 ggcgccggga gtatctgggc agcgaggagt tccgcagcat ggcgcagcag tacctcgagg
     1741 gagtgcggac ggatacgatg gggtgcgtga tcgagcatct tcagacgccc tctatccgtt
     1801 ccgaaggcat cgacggaagt gtgctgcgct actgggatga cgcccggaaa aagtgggcaa
     1861 cgatccggct tttaagaaca aataccggat atgagaaaat ctgccgtacg gtaaagctcc
     1921 gccaggagct cggacgctaa gaacatctat aaaaacggga ctgccgcata aagcggacag
     1981 tcccgtttga cgtgataagt aatataagga ggaagatcat cctatcgatt tcagatggaa
     2041 atgtagatgg aacattcgtt gaaaaaaatg cgcgcatttc ttcctgccgg atttttgcat
     2101 cgaattccgt gtctcttcca cgttttgaca atccacggaa tgtgtccgac aaaccgcaga
     2161 caatgagcgg attccctagt gtccctagcg gcactgcttc acagacaata taaaaccgcg
     2221 cgaaccgaac tcgccgtcat ctgctattga agttaaggca gatgacggct caaacagcgg
     2281 cttcgcgcgg ttatgtttta ttcagcagtg ccacaaggga cacacgagaa tcctaaaatt
     2341 gtctgcgggt tgcggacacg cccgtggagt gttcaaaact tggaaaaaca cggatcatcg
     2401 gagatgcaaa aaccggctgg ttagaaatgc cacatttttg atccgaattt ccagatgcat
     2461 tccatctgaa atcgaaggac atggtcattc ttcatactac ttaccacatc aaacaggact
     2521 ggctgtttga tgcgacagtc ccgttttgta tggaagagaa aagattactg tgtatttttc
     2581 tgcagttcat tggcattttc ctgaagctgc tgcatgatca gggcaatgtc gtccacgctt
     2641 tgcagattgg tgcggcagcc ggcaagggtc gcaccagcgc ttgcggtaac ttcctgtgtc
     2701 agggcggata cattttcgat gctctggata atctggctgt tggaatcaga taccgcatcc
     2761 acaccctgct ttaactcgga catgccggag gtaatgcttg cggtattttt ctcaatgaga
     2821 tcaaaatatc cggcagcgga agtaatgtat tcactctggt gcctgttgct gtcgaacagt
     2881 tcagtgacgg agtcggtaac ggaggaaatg gactgcgaga tgtgctcaat caactgattg
     2941 atgtctcccg tagcaccatt ggtctgaccg gcaaggctgg aaatctcctc ggcaacaacg
     3001 gcaaaaccgc gtccggcttc accggcgcgt gctgcctcaa tactggcatt gagcgctaaa
     3061 aggcttgtct gggaagcaac actgctgatt aaggcaacga tgctctgcat tttgtcagca
     3121 tattccttca gctcttccat agaatccgcg acgcgtgtac tggaagcctc tgagacctgt
     3181 acctgctgca caagattctt cataacatct ttgcttgccg tcagattggc agaggtctct
     3241 gcggtggagg agagaagttc ggcagtgacg gatgatacat tctgtacctg ctggtcaatg
     3301 ttttctgtct gctgttgctg cacctggata gcagcggcgg tctcggaagt gccgctggta
     3361 agatcctcca ttgcggtctg tgtgccggcg atggaatcac gaagctgtga ggtgctgtgt
     3421 gaaacctgat cgatatttcc tgccatatct ccggctacct ttaaggtcag cttgagaaga
     3481 tcatcggact gggtgcggtc ggagtccgcc tttttgatgt tggcatcgtt gatctgcgca
     3541 atttttgtga cagacatcaa tacaaaaata taggaaagga taatgcaggc gattataatc
     3601 tcggcattgg taatctgggc agcggtgaaa ccctgtgtaa cggcacgaaa gaccagaaca
     3661 aggatattga tcaccagtgc ataagtacac agcagggaaa cgagcttttt atctgcatac
     3721 accagaagaa tcaacagcat tacgattaca tagcagaacg tcagatctgt tgatgaggta
     3781 aacatcacat aggcatacag aagggcaaag ccgccgccgc agagatagcg cacactagag
     3841 gtatccttct gctttgtgta ggcaagaatg gatgtgacac agggcagcag acagagcaga
     3901 agaaaaatca gatacgacgg aagactgcgc acgcctttta agacttctgc gatgtaggcc
     3961 gccgcaagta ccgtattcat tgtgcaggtg ccggcaatgg ctgtcttatt gcggctttgt
     4021 gctgcaactg ccggatggga aagat
//
LOCUS       DBMDQJ010000146         4042 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8287_length_4042_cov_1.608982, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000146 DBMDQJ010000000
VERSION     DBMDQJ010000146.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8287_length_4042_cov_1.608982"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            125..1069
                     /locus_tag="ACZO9R_04125"
     CDS             125..1069
                     /locus_tag="ACZO9R_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR0912288.1"
                     /translation="MTKEEQFSFLSKDGKTTIHAVKWMPESGEYRAVLQITHGMVEYI
                     ERYKPFAEFLNDQGVLVVGHDHLGHGASVASEEELGYFAEPDPSDTLVEDMHTLRTMI
                     QAENPGVPYFMMGHSMGSYMLRKYLCIHPEGVAGAVIMGTGAMPDSTMKFGLFLCRTI
                     ALFRGWHYRSRLMQSLSYSKPYKRYDLYGKDYANSWLSKNVENVKAYYADPRCTFLFT
                     VNGYKGLMEAVLFDNQMENIKKVPKNLPLFFVSGADDPVGDLGEGVKKVYGMYKDAGV
                     SDITYRLYEDDRHEILNETDRDQVMADIAAWLNVHVEL"
     gene            1187..2269
                     /locus_tag="ACZO9R_04130"
     CDS             1187..2269
                     /locus_tag="ACZO9R_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HIR0912289.1"
                     /translation="MKKNGMRVTSLFLAAVMLASAPVTASAADTPANVEGEVVVDQTS
                     QDQTSAESQNQAGQDQTSAEAQDQSQASAAENAATGANSDNVTIQADDKPYLALGADL
                     TDAQKNTVLSLMGIDPANLGNYDVVYTTNAEEHQYLDSYISSSQIGSKSWSSVVIVKR
                     DKGNGLNISTRNINYCTVGMYKNALTTAGITDADIIVAGPQEISGTAALVGVFKAYQE
                     MTGETINADIIDASLNELVVTGQLEQSIAGLSDEEVEEFIAYIKSVIAQNDLKDEASI
                     NQAIDDACSKYNVTLSDSERQQIVDLLLKITSLGINLDSLVDYAESLYHSFKDGGGDS
                     SVLSSVGTFFSNVFSSIADFFKGLFS"
     gene            2291..3970
                     /gene="ade"
                     /locus_tag="ACZO9R_04135"
     CDS             2291..3970
                     /gene="ade"
                     /locus_tag="ACZO9R_04135"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HIR0912290.1"
                     /translation="MVDRKRRLDCANGIRKADIVLKHGKVLNVFTEEILEADVAICGD
                     TIVGVGSYEGEREIDCTGKYLVPGFLDAHMHIESSMVSPGELSKVLVQAGTTTIVADP
                     HEIVNVCGVKGMDYFLKCAGKALVHIFFMIPSSVPATDVETNGCGEFLASDMMKYVDD
                     ARVLGLGETMRFVECYQGERRMSDKLELFAKKHIDGHAPGITGKAVQAYRLAGVENDH
                     ECANSEEVLDKLRAGFHIYIREGSGAKNLETLIRTLLDANVALDQCAFCTDDKHIEEI
                     RKEGHISTCIRKAIALGVPVAKAYKMGSYQPAMFYGLKGYGAIGAGYHADIVFLDNLE
                     EVRPTDVMKDGRILGEEDYKKDYWVPVPEELLHTVHVGELSRERIQLACNGKTDVIQM
                     NAHQIVTTHLVEEVPTENGYFVPNEVYNKICVVERHGKTGEIGVAPLKGFGVKGGAVA
                     TSVAHDSHNLIVAGDNDDDILLAIRTVEEQQGGYAIVSGGKVIDVLPLPIAGLMSDRR
                     YEEVTEKLSAMQKETRELGISENVDPFVTLSFMALTVIPEIRVTERGVYLF"
BASE COUNT         1072 a          819 c         1221 g          930 t
ORIGIN      
        1 atgtctgcaa gagcatcctg cagtcggcag gggaagccgg atgatactgg ttttattgca
       61 aaagatacag agcagagagt aggaagcaga gagtataaat aataaaaagt gagggaacaa
      121 tgctatgaca aaagaagaac agttcagttt tttatcaaag gacggaaaga caacgattca
      181 cgcggtaaag tggatgccgg agagcgggga gtaccgggcg gtcttgcaga tcacgcacgg
      241 catggtggag tatattgaac gctataagcc gttcgcggaa tttttaaatg accagggggt
      301 ccttgtggtc ggacatgatc atctgggaca tggtgcgtcg gttgcatccg aggaggaatt
      361 gggatatttt gcagagccgg atccgagtga cacgctcgtc gaggatatgc acacgctgcg
      421 cacgatgatc caggcagaga atcccggcgt gccgtatttt atgatgggac acagcatggg
      481 atcttacatg ctgcgcaaat acctgtgtat tcacccggag ggagtggctg gcgccgtcat
      541 catgggaacc ggggcaatgc cggatagcac gatgaagttt ggactgtttt tatgccgtac
      601 gatcgcacta ttccgtggat ggcattacag aagccgcctg atgcagagcc tttcctacag
      661 caagccgtac aagcgctacg atttatatgg aaaggattat gccaacagct ggctttccaa
      721 gaacgtggag aatgtcaagg catactatgc tgatccgcgc tgtacttttc tgtttaccgt
      781 aaacggctac aaaggcctga tggaggcggt attgtttgac aatcagatgg agaatataaa
      841 aaaggtcccg aaaaatctgc cgctgttttt tgtatccggt gcggatgatc cggtcggaga
      901 tctgggagag ggagttaaga aggtgtacgg gatgtacaaa gatgcggggg tttcggatat
      961 tacctaccgc ctgtacgagg atgaccgcca tgagatttta aacgagacag accgcgatca
     1021 ggtcatggcg gatattgcgg cttggctgaa cgtgcatgtg gaactgtaat cttgccggac
     1081 aggtttctta aatttatata aataaaacta ctggagggaa aacgccggtt gtttttaagg
     1141 aaagttcgta gtaaaataga gacttgtttt ggaaggagga tttcacatga aaaagaatgg
     1201 aatgagagtt accagtttat ttctggctgc ggtgatgctt gcatcggcgc ctgtgacggc
     1261 atccgcggcg gatacaccag cgaatgtaga gggagaggtt gtcgtagacc agacaagtca
     1321 ggatcagact tcagcagagt cgcagaatca ggcagggcag gatcagactt cggcagaggc
     1381 gcaggatcag tcacaggctt cggcagcgga gaacgctgcc acgggagcaa acagtgataa
     1441 cgtgacaatc caggcggatg ataagccata tcttgcgctg ggagcggatc tgacggatgc
     1501 ccagaagaat acggtgcttt ccctgatggg gattgatccg gcgaaccttg gcaattatga
     1561 tgtggtctat acaacgaatg cagaggagca tcagtatctg gattcctata tcagttcctc
     1621 acagatcgga agcaagtcat ggtcttccgt tgtgatcgta aaacgcgata agggaaacgg
     1681 attaaatatt tcaaccagga atatcaatta ctgtacagtc ggcatgtaca aaaatgcgtt
     1741 gacaacggcg ggtattaccg atgcggatat catcgttgca ggaccgcagg agatctccgg
     1801 tacggcggcg cttgtcggcg tattcaaggc ataccaggag atgacaggtg agacgatcaa
     1861 tgcggatatc atcgacgcat ccttaaatga gctcgtggtt actgggcagt tagaacagtc
     1921 gattgccgga ctttccgatg aggaagtgga agagtttatc gcttacatca agtcggtcat
     1981 tgcacagaat gatctgaaag atgaggcgag catcaatcag gcaattgatg atgcctgcag
     2041 caaatataat gtgacactgt cagattccga gcgtcagcag atcgtggatc tgctcttaaa
     2101 gattacatct ctgggaatca atcttgacag ccttgtggat tatgcggagt cactgtacca
     2161 ttcctttaag gatggcggag gagacagcag cgtgctctcg agcgtaggca catttttctc
     2221 aaatgtattc agctcgatcg cggacttctt taagggattg ttttcgtaaa cggaggagag
     2281 gaaggaatct atggtagaca gaaagagacg gctggattgc gcgaacggaa tccggaaagc
     2341 agacattgta ttaaagcacg gcaaagtatt gaacgtattc acggaggaga ttctggaagc
     2401 ggatgtggca atctgcggcg atacgatcgt gggagtcggt tcctacgagg gtgagcggga
     2461 gatcgactgt accggaaaat atctggtacc gggatttctg gatgcccata tgcatattga
     2521 atccagtatg gtatctccgg gcgaattgtc caaggtgctg gtgcaggcgg gaacgacgac
     2581 cattgttgcg gatccgcacg agattgtcaa tgtgtgcggt gtgaagggaa tggattattt
     2641 tctgaaatgt gcgggcaagg cgctggtaca tattttcttc atgattccgt cctctgttcc
     2701 ggcaaccgat gtggagacaa acggatgcgg ggagtttctg gcgtccgata tgatgaagta
     2761 tgtggatgat gcgagagtgc tgggactcgg ggagaccatg cgttttgtgg agtgttacca
     2821 gggcgagcgc cggatgtcgg ataagctgga actttttgca aagaagcaca tcgacggaca
     2881 tgcgccgggc attacaggaa aggcggtaca ggcgtaccgt ctggctggcg tggagaatga
     2941 tcatgagtgt gcgaactccg aggaagtgct cgacaagctg cgcgccggtt ttcacatcta
     3001 tatccgtgag ggaagcggtg ccaagaatct tgaaacgtta atccgcacgc ttttagatgc
     3061 caatgtggcg ctcgaccagt gcgcattctg caccgacgat aagcatatcg aggaaatccg
     3121 caaggaagga catatcagca cctgtatccg caaggcgatt gcacttgggg ttccggtggc
     3181 aaaggcatac aagatgggaa gctaccagcc ggcaatgttt tacgggctca agggatacgg
     3241 tgccatcgga gctggatatc acgccgacat tgtgttcctc gataatctgg aggaggtgcg
     3301 tccgaccgat gtgatgaagg acggcaggat cctgggagag gaagactata agaaggatta
     3361 ctgggttcca gtgccggagg aactgcttca cacggttcat gtcggggagc tttccagaga
     3421 gcggattcag cttgcatgca acggaaagac cgatgtgata cagatgaatg cgcatcagat
     3481 cgtgacgacg catctggtgg aagaagtgcc gacagaaaac ggatattttg tcccgaatga
     3541 agtatacaat aagatctgtg tggtggaacg ccacggcaag accggagaga tcggtgtggc
     3601 accgctcaag ggctttggcg taaaaggcgg tgccgtagcg acgtccgttg cgcacgattc
     3661 ccacaatctg attgtggcgg gagacaatga cgatgacatt ctgcttgcca tccggactgt
     3721 ggaggagcag cagggcggct atgcgatcgt gtccggcgga aaggtaatcg atgtactgcc
     3781 gctgccgatc gcgggtctta tgagcgacag acggtatgag gaagtcacgg aaaagctctc
     3841 tgccatgcag aaggagacga gagaacttgg tatctcagag aatgtagatc cgtttgttac
     3901 cctttctttt atggcgctta cggtaatccc ggagatccgt gtcacggagc gcggtgttta
     3961 tttattctga tgaacagatt atgaaggaga tttatgcagg aaaagattca aattatcagc
     4021 gacgggtcat tagacctttc aa
//
LOCUS       DBMDQJ010000147         4039 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8297_length_4039_cov_1.499746, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000147 DBMDQJ010000000
VERSION     DBMDQJ010000147.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8297_length_4039_cov_1.499746"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(29..1792)
                     /gene="aspS"
                     /locus_tag="ACZO9R_04140"
     CDS             complement(29..1792)
                     /gene="aspS"
                     /locus_tag="ACZO9R_04140"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR0912291.1"
                     /translation="MTQSRTHNCNELRLSNAGEKVTLVGWYENLRKVSKNLGFLILRD
                     FYGTTQIVVETEEMMEKLDGVNKESTISVTGTVRERSNKNTELPTGEIEVVPEGIEIL
                     GKCIYNALPFEINQSREADENTRLKYRYLDLRNPSVKSKIVLRSRIVAELRNKMNELG
                     FLEITTPILTCSSPEGARDYLVPARNHPGKFYALPQAPQQFKQILMASGFDRYFQIAP
                     CFRDEDARADRSPGEFYQLDMEMAFASQEDVFSVVEEVLPPVFEKYGVYKTASKAPFV
                     RIPYLESMDKYGTDKPDLRIDLVLQDATDVMADCGFGPFEGQTIKAVVVDGFTATRKV
                     IDGICDEVEVQTANKAYWFKLDENGEFVGGISKFLQERKEKVIEALGLKPGDFIGLTA
                     GKKLAAQKTAGVLRRLLGAASEKHMKKDCYEFCWIVDFPMYEIGEESGELEFCHNPFS
                     MPQGGMNALCNEEPLSILAYQYDLVCNGVELSSGAVRNHDPEIMIKAFELVGLGEEDV
                     KAKFPAMYNAFCYGAPPHAGIAPGVDRMIMLICGEESIREIIPFPMNKNAMDIMMGAP
                     ATVDQKQLDDVHIRIQMPEEK"
     gene            complement(1912..2658)
                     /locus_tag="ACZO9R_04145"
     CDS             complement(1912..2658)
                     /locus_tag="ACZO9R_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR0912292.1"
                     /translation="MTIWEILGISQTTDQKQIKKVYAARVKSCHREEKPEEWAVLHDA
                     YQRAIQYAKRQEKESDLNGSRTVPSPVKPVAREGIRERDAALEKEYGQSETDETEAQW
                     QEYFARIRAGQERSRKMLTEELPERLRGLTLQGEGTQKKRWEEFLQSELAITLYKEPE
                     FWRQVGEHVRAQEWSEEACRYIGRRVEDIKYEFNSRDYPELWRVLDELERICLEEALK
                     DPLRRTKITLRGFGLTILVFVLIGVFVRII"
     gene            complement(2665..>4039)
                     /locus_tag="ACZO9R_04150"
     CDS             complement(2665..>4039)
                     /locus_tag="ACZO9R_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR0912293.1"
                     /translation="YQCRSQMEDAVMLVFDFGGGTLDVSLVECFDNIVEILAVSGDNH
                     LGGSDFDRLIADMFWKSCGLSENRFSAEDAAIVLERAENCKFELTGQETAQMCVTLAG
                     REYRMEVSRKELILQAEPLFERMAQPIRRVLSDGQKSAREVDAVVLVGGSCKMPVVQK
                     YLNYLLQRTDIAVVSPDYMIALGAGVYAGIKERNADIRDMLLTDICPFSLSVSQYNEA
                     DPNRDLCDVLIERNMALPVSRETIMTTAHDRQTKMRFKVYQGEAMYAEDNLLLGSITV
                     PVPVGPEGKEKVRVRYTYDINGILVVDVHVVSTGQEKQLVIRNDKIKLSEKELKKRLE
                     QLNKLKIHPRDLEENRLVLARAEKLHRETTGYIREQIDSRVQYFNYYLGRQDVLSINK
                     GRKQLNLFLDYVEEQCLQEAAFEGTADGFSRWYESSKQAVDAQDQVDYRKEEQEYELW
                     RGGHLTS"
BASE COUNT          883 a         1223 c          812 g         1121 t
ORIGIN      
        1 cctgtgtctt tattgctgtc gctttacgct acttttcttc cggcatctga atgcggatat
       61 gaacgtcgtc cagctgcttc tgatctacag tcgccggtgc tcccatcatg atatccatcg
      121 cgttcttgtt catcgggaac gggatgatct cacgaatgct ctcctcgccg cagatcagca
      181 taatcatacg gtcaacaccc ggagcgattc ctgcgtgtgg cggtgctccg tagcagaatg
      241 cattgtacat ggcagggaat tttgccttca catcctcctc gccgagacca accagttcaa
      301 atgccttgat catgatctcc gggtcatggt tacgaaccgc accggaggaa agctctacgc
      361 cgttgcacac cagatcgtac tggtaagcaa ggatggaaag cggctcctcg ttgcagagtg
      421 cgttcatacc gccctgcggc atggaaaacg ggttgtgaca gaactccagt tctccggact
      481 cctcgccgat ctcatacatc gggaaatcta cgatccagca gaattcgtag caatcctttt
      541 tcatgtgctt ctcgctcgca gcgccaagca gtctgcggag tacgcctgcg gtcttctgcg
      601 ctgccagctt cttgccggcg gtcagaccga taaaatcacc cggctttaat ccaagtgcct
      661 caatcacttt ttccttgcgc tcctgcaaga acttggaaat acctcccaca aattcgccgt
      721 tctcatccag cttgaaccag taagccttgt tggctgtctg cacttccacc tcgtcacaga
      781 ttccgtcgat cacttttctg gtcgcagtaa atccgtccac gacaaccgct tttatggtct
      841 gcccctcgaa cggaccgaat ccgcagtctg ccatgacgtc cgtcgcatcc tgcaatacca
      901 gatcaatgcg gagatccggc ttgtcggtgc cgtacttgtc catggactcc agatatggaa
      961 tacgcacaaa cggagccttg gaagcagtct tgtatacacc atacttttca aataccggcg
     1021 gcagtacctc ctcgacaaca gagaacacat cctcctggga agcaaatgcc atctccatat
     1081 cgagctgata gaactctccc ggagaacggt ctgctcttgc gtcctcatca cggaaacacg
     1141 gtgcaatctg gaaatagcgg tcgaatccgg acgccattaa aatctgcttg aactgctgcg
     1201 gtgcctgcgg cagtgcatag aacttgcccg gatgatttct ggccggtacc agataatctc
     1261 tcgcgccttc cggggaagag caggtcagaa tcggagtcgt gatctctaaa aatcccagct
     1321 cgttcatctt attgcggagc tctgccacga tgcggcttct gagcacgatc ttgctcttga
     1381 ccgacggatt tctcagatcc agatagcgat attttaatct ggtgttctca tccgcctctc
     1441 tggactggtt aatctcaaac ggaagtgcat tgtagataca ctttccaagg atctcgatcc
     1501 cttccggcac gacttcaatc tctccggtcg gaagctctgt attcttattg gagcgctctc
     1561 tcacggttcc tgttacggaa atggtggact ccttgttcac tccgtccagc ttctccatca
     1621 tctcttctgt ctcaacgaca atctgggtcg ttccatagaa atctcttaaa ataagaaatc
     1681 ccaggttctt gctgaccttg cggagattct cgtaccatcc gacgagcgtt actttctctc
     1741 ccgcattgga aagacgcagc tcattacagt tatgtgttct cgactgtgtc attttcttaa
     1801 ctcccttgta tcaatctaaa aatattcttc aaatctgtcc gggcagcagc ctgtccggcg
     1861 ctttttttat cttacaccat gaaacgcgat ttttcaacgg ttttatccgt tctaaataat
     1921 tctgacaaac actccgataa gcacgaacac aaggatcgtc aatccaaatc cacgcagcgt
     1981 tatctttgtt ctccgtaacg gatctttcag tgcctcctcc agacaaatcc tctcaagttc
     2041 atccagcaca cgccataatt ccggataatc acgactgtta aactcatatt ttatgtcttc
     2101 cacgcgtctg cctatgtagc ggcatgcttc ctccgaccac tcctgtgccc tcacatgttc
     2161 accaacttgt ctccagaact ctggttcttt gtacagagta attgccaact ccgattgcag
     2221 gaactcttcc cagcgtttct tctgggttcc ttccccctgc aatgtgagac ctcgcagccg
     2281 ctccggcagc tcctctgtca gcattttcct gcttctctcc tgtccggcac gaatgcgtgc
     2341 aaaatactcc tgccattgtg cttctgtctc atcggtttca gactgtccgt actccttctc
     2401 cagcgccgcg tcccgctctc ttataccttc ccgtgccacc ggtttcaccg gacttggaac
     2461 cgtcctgctt ccgttcaggt cactttcttt ctcttgtctt ttcgcgtatt gaatggcgcg
     2521 ctggtaagca tcgtgaagca ctgcccactc ctctggcttt tcctccctgt gacaactctt
     2581 tactctggca gcatacactt ttttgatctg tttttgatct gtagtctggg aaattcccag
     2641 tatctcccat attgtcatat tcttctagct ggtcagatgt ccgcccctcc acaattcata
     2701 ttcctgttcc tctttgcggt aatccacctg atcctgcgca tcgaccgcct gcttactact
     2761 ctcataccac cgggaaaaac cgtctgctgt tccctcaaat gccgcctcct gcagacactg
     2821 ctcctccaca tagtccagaa acagattcag ctgttttctg cccttgttga tggagaggac
     2881 gtcctgtctt cccagataat agttaaaata ctgtacacga ctgtcaatct gttcccggat
     2941 atatcccgtt gtctcacgat gcagcttttc agcacgcgca agcaccagcc ggttttcctc
     3001 aagatctctc ggatgaatct tcagtttatt caactgctcc agtctttttt tcagttcctt
     3061 ttctgataat ttgattttat cattccggat cacgagctgt ttttcttgtc ctgtcgatac
     3121 gacgtgcaca tctaccacca gaattccatt gatatcgtaa gtataacgca cccgcacctt
     3181 ctcttttcct tccggtccga caggaaccgg aaccgtgatg cttcccaaca gcagattgtc
     3241 ttccgcgtac attgcttccc cctggtatac cttgaacctc attttggtct gcctgtcatg
     3301 tgccgtcgtc ataatagtct ccctgctgac cgggagcgcc atgttcctct caatcagcac
     3361 atcacagaga tcacggttcg gatctgcttc gttgtattgg gacacgctga gagaaaacgg
     3421 acagatatcc gtaagaagca tatcgcgaat atccgcattg cgttccttga tgcctgcata
     3481 cactcctgcg ccgagcgcaa tcatataatc cgggctgaca accgcaatat ccgtgcgctg
     3541 caacagataa ttcaaatact tttgcaccac cggcatttta caggaccctc caacaagcac
     3601 taccgcatct acttctctgg cactcttctg cccgtccgac aacacacgcc ggatcggttg
     3661 tgccatccgt tcaaacagcg gctccgcctg caggatcagc tcctttctgg agacctccat
     3721 ccgatattct ctcccagcaa gtgtcacaca catctgtgca gtctcctgac cggtcagttc
     3781 aaacttacag ttttccgcac gttccaacac aattgccgca tcctcagctg agaagcggtt
     3841 ttcagaaagt ccgcacgact tccagaacat atctgcaatc aaccggtcaa aatcactgcc
     3901 gccgagatga ttgtctccgc tgaccgccag aatctccaca atgttatcaa aacactctac
     3961 taatgacaca tcgagcgttc cccctccaaa atcaaacacc agcatgacgg catcctccat
     4021 ctggcttcgg cactgataa
//
LOCUS       DBMDQJ010000148         4028 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8329_length_4028_cov_1.750191, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000148 DBMDQJ010000000
VERSION     DBMDQJ010000148.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8329_length_4028_cov_1.750191"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="ACZO9R_04155"
     CDS             <1..373
                     /locus_tag="ACZO9R_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR0912294.1"
                     /translation="FLREHKGELVVIGAVTFLQRSTVFLITGMVYLGFGLSGSSLFHI
                     LAVQAAVYIAVDMLPVPGAQGITELMYAAVFGGVFGAQYVKASMVLVRGMDFYVPFCV
                     SMLIVLAGHGVSYVRRQRKRA"
     gene            complement(376..1242)
                     /locus_tag="ACZO9R_04160"
     CDS             complement(376..1242)
                     /locus_tag="ACZO9R_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0912295.1"
                     /translation="MNSCITCKEAIEHCMSTHTFAFAHLYGDEKIMDMHIHNCCEVYY
                     SISGGKQFLIDHCLYDIRPGDLFLINQFESHHLTQIDQQTHERIILSIDPEYLSKLST
                     GVTNLGHCFSFRDTRMPHQIHLSPKEQNRFMYFIHELHDDGGMGEDLLDQAIFLELMV
                     FLNRAFAARCTDAAAPAPAPSSNYHAQVDAILSYINSHIGEPLTLAGLSAHFFMSTSY
                     LCRIFKEATGTTINKYITAKRITLSKSLLDQGCSVSEACEACGFNDYSNYLRAFTKAV
                     GISPKKYAQFHS"
     assembly_gap    1254..1263
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1482..2276
                     /locus_tag="ACZO9R_04165"
     CDS             1482..2276
                     /locus_tag="ACZO9R_04165"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HIR0912296.1"
                     /translation="MVNFSLEGKVALVTGASYGIGFAIASAYAQAGATIVFNDIKQEL
                     VDKGLAAYEELGIKAHGYVCDVTDEEQVNALVAKIEKEVGVIDILVNNAGIIKRIPMC
                     EMSAAEFRQVIDVDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRETVSAYAAA
                     KGGLKMLTRNIASEYGEYNIQCNGIGPGYIATPQTAPLREIQPDGSRHPFDQFIIAKT
                     PAARWGSAEDLQGPAVFLASDASDFVNGHVLYVDGGILAYIGKQPQ"
     gene            2489..>4028
                     /locus_tag="ACZO9R_04170"
     CDS             2489..>4028
                     /locus_tag="ACZO9R_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR0912297.1"
                     /translation="MSREKAEWSPEWLTIREFIHITPVNLFHKEQEEGEKQPGAEQQL
                     TAEFRKNLHVLVRARFTLETAQENLTLRLTADDHYKLYVESGFVAEGPAPGYPDHYYY
                     NEIPLGKMEAGEHCFGMHLYYQGLINRVYNSGDLRFAFAAELMDAQGCRVPLRFCYKR
                     TDAYSGGTIGYDTQFLENFDSRKFPEGWSSAEFDDAGWKTPAAAEWADYRLYLQPTRM
                     LCGERIKPEKIEICEDGSIRIDVGEEVAGSLCLTAEGSAGDTVEIFCGEELDESGSVR
                     CEMRCNCSYHEIWTLTDGCCSLEPYDYKGFRYAKLLPGKGVNIRGIFVQTRHYPMEEG
                     AEVMSSEKRLEQIFSICKNAVKYGTQEGYVDCPTREKGQYLGDAVVTAHAQVLLTGEM
                     QMLLKCIDQFAQTRAVCPGLLAVAPGGLMQEIADYSLMYPQLLALYYRYTGDAAALLP
                     YYETALGMIRHFAQYERADGLLVQVADKWNLVDWPENLRDDYDFALTRPVVGAGCHNV
                     INALY"
BASE COUNT         1002 a          867 c         1249 g          900 t
ORIGIN      
        1 ttttttaaga gagcacaaag gggaactggt tgtgattgga gcggtgacat ttttacagag
       61 aagcacggtg tttctgatca cggggatggt gtaccttggc ttcggacttt cggggagcag
      121 tctttttcac attctggcag tgcaggcggc ggtgtacatt gcggtggaca tgcttccggt
      181 tccgggtgcg cagggaatca cggagcttat gtatgcggcg gtgttcggcg gagtgtttgg
      241 cgcacagtat gtgaaggcgt ccatggtgct ggtgcgcggc atggatttct atgtgccgtt
      301 ttgcgtcagc atgctcatcg tgcttgctgg gcacggcgtt tcatatgtga gaagacaaag
      361 gaaacgtgca taacctcagg agtgaaactg cgcgtacttt ttcggagaga tgccgactgc
      421 ttttgtgaag gcacgcagat aattgctgta gtcgttgaag ccgcaggcct cgcaggcttc
      481 cgagacggag cagccctgat cgagcagcga tttggacagg gtgatgcgct ttgcggtgat
      541 gtatttgttg atggtggtgc cggtggcctc cttgaaaata cggcacagat aggacgtgct
      601 cataaaaaaa tgcgcggaca gaccggcgag cgtcagcggc tcgccgatat gggagttgat
      661 gtaggataaa atggcgtcca cctgggcgtg gtaattggaa gatggagccg gggcgggggc
      721 tgcggcgtct gtgcagcggg cggcgaaggc tctgtttaaa aagaccatca attccagaaa
      781 gattgcctga tccaagaggt cttcccccat gccgccatcg tcatgcagct cgtgaataaa
      841 gtacataaag cggttctgct ccttggggga gaggtggatc tggtgcggca ttctggtgtc
      901 acggaaagaa aaacaatggc caagattcgt cacgccggtg gagagcttcg ataagtattc
      961 cgggtcgatg gagaggatga tccgctcgtg tgtctgctga tcgatctgtg tcaggtggtg
     1021 gctttcaaat tggttgatta agaaaagatc gccggggcgg atgtcgtaca ggcagtggtc
     1081 gatcagaaac tgttttccgc cggaaatcga gtaatagact tcgcagcagt tgtgaatgtg
     1141 catgtccatg atcttctcat cgccgtagag atgggcgaac gcgaatgtgt gggtgctcat
     1201 gcagtgttcg atggcttctt tgcaggttat acagctattc ataggttctc cgtnnnnnnn
     1261 nnngcagtgc cgccgttttt aatgcgccag tggcgccatg tgccttgtgg tgttatgtgt
     1321 tctgtagcgc atgtctcttt acacagcata ggacaaaagt tcaatatttg caagatttat
     1381 aatacaatat tgcgggaaat ggaataacat atctgatata ctaggtgtaa gcaatgagca
     1441 gtaccagagt aataagaggg aaaaagaaag gacggaaaac tatggttaat ttttcattag
     1501 agggaaaagt agcgcttgta acaggcgcat cctacggcat cggattcgca atcgcatctg
     1561 catatgcgca ggcgggcgca acgatcgtat tcaacgacat caagcaggag cttgtcgaca
     1621 agggacttgc cgcttatgag gagctgggca tcaaggcgca cggatatgtc tgcgatgtca
     1681 cagatgagga gcaggtaaac gcactggttg caaagatcga aaaagaggtc ggcgtgatcg
     1741 acattctggt aaacaatgcc ggcatcatca agcgtattcc gatgtgcgag atgtccgcag
     1801 cagagttcag acaggtcatc gatgtggatc tgaacgcacc gtttatcgtt tccaaggcgg
     1861 tgattccgtc catgatcaaa aaaggacacg gcaagatcat caacatctgc tccatgatgt
     1921 ccgagcttgg ccgtgagact gtatccgctt atgcagcggc aaagggcggc ttaaagatgc
     1981 tgaccagaaa cattgcatcg gagtacggcg agtacaacat ccagtgcaac ggtatcggac
     2041 cgggctatat cgcgactccg cagaccgcac cgctccgcga gatccagccg gacggaagca
     2101 gacatccgtt tgaccagttc atcatcgcaa agactccggc agcaagatgg ggaagcgccg
     2161 aggatttaca gggaccggca gtattcttag cttcggatgc atctgatttt gtcaacggac
     2221 acgtactgta tgtggacggc ggtatccttg cttacatcgg aaaacagccg cagtaagtaa
     2281 tttcaggcag cataaaaatg acatagaaat agtgtatttt ttcataagaa agtctatgga
     2341 aaaatgcact atttttttat accataataa aaaatcagga agctgtctgt aagggaatct
     2401 gtgcagtacg actgccaaga agaaggaatc agaaaagctg cagacagaag aacaaacgcc
     2461 cggaaaggat ggcggggtga cagaaaagat gagcagagaa aaagcggaat ggagtccaga
     2521 gtggcttacg atccgcgaat ttatacatat cacaccggtt aatctgttcc acaaggagca
     2581 ggaggaaggg gaaaaacaac cgggggcaga gcaacagctg acagcggaat tccggaagaa
     2641 tctgcatgtt ctggtgcgtg cgcgttttac gcttgagacg gcgcaggaga acctgacgtt
     2701 aagactgaca gcggatgacc actataagct gtacgtggaa tccggatttg tggcggaggg
     2761 acctgcgccc ggttatccgg atcactacta ttacaacgag attccactgg ggaagatgga
     2821 ggccggagag cactgcttcg gaatgcactt atattatcag gggttaatca accgtgtata
     2881 caacagtggc gacctgcgct ttgcgtttgc tgcggagttg atggatgcgc agggctgccg
     2941 ggtaccgctt cgtttttgct ataagaggac ggatgcgtac agcggcggaa cgatcggata
     3001 tgatacgcag tttctggaaa actttgacag cagaaaattc ccggaaggat ggagcagtgc
     3061 ggaatttgat gatgccggat ggaagacgcc ggctgcggca gagtgggcgg attaccggtt
     3121 gtatttacag ccgacgcgga tgctttgcgg ggagcgcatc aagccggaaa aaatcgagat
     3181 ctgtgaggat ggcagcatcc ggatcgatgt gggagaggaa gtcgccggaa gcctgtgcct
     3241 gacggcggag ggaagcgcgg gggataccgt ggaaatcttc tgcggagagg aactggatga
     3301 gagcggcagc gtccgctgcg agatgcgctg caactgctcc taccacgaga tctggacgct
     3361 cacagacggt tgctgcagtc tggaaccgta tgattacaag gggttccgct acgcaaagct
     3421 gcttccgggg aagggcgtga acattcgcgg gattttcgtg cagacacgcc actatccgat
     3481 ggaggagggc gcagaggtga tgtcctccga aaagcggctg gagcagattt tttccatctg
     3541 taagaacgcg gtaaaatacg gaacacagga gggctatgtg gattgcccga ccagagaaaa
     3601 aggacaatat ctcggggatg ccgttgtgac ggcgcatgcg caggtattgc tgaccggaga
     3661 gatgcagatg cttttgaaat gcatcgatca gtttgcgcaa acgagggcag tctgcccggg
     3721 gcttctggcg gttgcgccgg gaggactgat gcaggagatc gccgattatt ccctgatgta
     3781 tccgcagctg ctggcactat actaccgcta caccggcgac gcggcggcgc tgcttccata
     3841 ctatgagacg gcgcttggca tgatccggca ttttgcgcag tacgagcgcg cggacgggct
     3901 gcttgtgcag gtggcagaca agtggaatct ggtcgactgg cccgaaaatc tgcgggatga
     3961 ttatgatttt gcgctgacaa gacccgttgt gggggcgggg tgccacaacg tcatcaatgc
     4021 cctgtatc
//
LOCUS       DBMDQJ010000149         4019 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8352_length_4019_cov_1.947422, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000149 DBMDQJ010000000
VERSION     DBMDQJ010000149.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8352_length_4019_cov_1.947422"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="ACZO9R_04175"
     CDS             complement(<1..542)
                     /locus_tag="ACZO9R_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0912298.1"
                     /translation="MATIKEIAELAKVSPATVSRVLNQDETLTVTAEVRNQILKIAHE
                     LNYIPPKMRHAQNKTHVTIGVADWHLVRGDRHDIRLASLDHIAAAMPQKADVSFLRIA
                     CREDADVDGILAFGSFSEEEINFLRKQSDAILFVNSDQKDYEFDRIIMDYEQGLKDMV
                     HYLLDVKEYRSIGYIGGIYE"
     gene            complement(734..1378)
                     /locus_tag="ACZO9R_04180"
     CDS             complement(734..1378)
                     /locus_tag="ACZO9R_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0912299.1"
                     /translation="MNGLHPIKMPSAKEKVAAELRKAILSRQLQEGEAVTLESVANQL
                     EVSVMPVREAFQILARDGLIKLQRNKGAVVLGINETYIREHYQLRAILESAAAEMCCA
                     PEVDITRIEESYECAAESIQNNDFAAYADQNREFHSEIWSAAGNSRMENMIAELWNGL
                     SMGSMITEADYAKVSIEEHERIMKAIRERNGAAAKEEMYRHIMRSRDDMLTNYR"
     gene            complement(1375..2010)
                     /locus_tag="ACZO9R_04185"
     CDS             complement(1375..2010)
                     /locus_tag="ACZO9R_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0912300.1"
                     /translation="MNKRTADYIADQLADQILSGAIQGGTPLKQEELSGLLGASRIPV
                     REALQIIEHQGLAVRLATRHVVTADFSDAHIREIYGIIGDIERRVLCDLAASEQQGEN
                     PGIFVEETLTELEFHRRIYGMTDNAYVRNILKNAVECYVRFAISLPRQETGSADAEYR
                     KGTEWQDGFWQKQEVKSRQLSADELRGAAAQMEPYYEELAARVVTERGKHQ"
     gene            complement(2040..3209)
                     /gene="alr"
                     /locus_tag="ACZO9R_04190"
     CDS             complement(2040..3209)
                     /gene="alr"
                     /locus_tag="ACZO9R_04190"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR0912301.1"
                     /translation="MESTLRRTWAEIDLDALAHNYHKLRERIGADVKFLGVVKADAYG
                     HGSVQVSHLLQELGADYLAVSSIDEAVELRLNDVTMPILILGHTPKEQVSRLIEYHIT
                     QAVSCEAKALEYSEEAVKCGGILKIHIKVDTGMSRLGYLCDGDHFETGVAGICHGCSL
                     PGLDAEGIFTHFAVSDEPGEDCKKYTEHQFDLFMRVIEEVEKRLGRKFALRHCANTGA
                     VARYPETFLDMVRPGLLLYGYGEFADELGLLPVMTLKTTVSTIKIYPAGTAISYGGIF
                     KTEHTTRIGVVPYGYADGFFRCLSNRCALMTKEGPAPQRGKICMDMCMIDLTGKMGVD
                     VGSEVEVFGKQNSINDLAALAGTIPYELTCAVSKRVPRIYIGNGKVVEKELLLRG"
     gene            complement(3870..>4019)
                     /locus_tag="ACZO9R_04195"
     CDS             complement(3870..>4019)
                     /locus_tag="ACZO9R_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR0912302.1"
                     /translation="DIPSPANPPAGCKFHTRCSECMEICKHEEPQVKDVGNGHKVKCH
                     LYDEK"
BASE COUNT          907 a         1199 c          829 g         1084 t
ORIGIN      
        1 ttctcataga taccgccgat ataaccgata ctccggtatt ccttgacatc cagaagatag
       61 tgtaccatat cctttaagcc ctgctcatag tccatgataa tccggtcaaa ctcataatct
      121 ttctgatccg aattgacaaa caggatcgca tcactctgtt ttcgcaggaa atttatctct
      181 tcctccgaaa aagatccaaa tgcgagaatc ccgtcgacat ctgcatcttc cctgcaggca
      241 atccgtaaaa agctgacgtc cgctttctgc ggcatcgccg ctgcaatgtg gtctaacgat
      301 gcgagtcgta tatcatgccg gtcccctctt accagatgcc agtctgccac accgatcgta
      361 acatgggtct tattctgcgc atgacgcatc ttaggcggaa tatagtttaa ttcgtgggca
      421 atctttaaaa tctggttcct gacttctgct gtgacggtca atgtttcatc ctgattcagt
      481 accctcgata cagtcgccgg tgataccttt gctaactctg caatttcttt gattgttgcc
      541 atataatcac gcctttcttc tgcattatat ttcacgtttt ttttaatttc aatagcgtcg
      601 gtaaactttt actaaatttt tacctgattt tacacgacct atacattagg cttataaatc
      661 gacggttttt gacttataaa aaagaccgga aatgccatat tctgacatct ccggtccgtc
      721 cgcccgtttc ttctcaacgg taatttgtca gcatatcgtc cctgcttcgc atgatatgac
      781 gatacatttc ttcctttgca gccgctccgt ttctctcacg aatcgccttc atgatgcgct
      841 catgctcctc gatcgacacc tttgcataat ccgcttccgt aatcatgctg cccatcgaca
      901 acccgttcca caattcagca atcatatttt ccatacggct attacccgcc gcactccaga
      961 tctcactgtg gaattcacgg ttctgatccg cgtacgccgc gaagtcgttg ttctgaatgg
     1021 actctgcggc acactcgtag gactcctcaa tccgggtaat atccacctcc ggcgcacagc
     1081 acatctctgc cgcagcactc tctaagatcg cgcgcagctg gtaatgctct ctgatatatg
     1141 tctcgttaat ccctaaaacg accgctccct tgttgcgctg aagcttaatc agtccgtcac
     1201 gcgcaagaat ctggaacgcc tcacgcaccg gcatgaccga cacctccagc tgattcgcca
     1261 cactctctaa cgtgacagcc tccccttcct gcagctggcg ggagagaatc gccttccgaa
     1321 gttccgctgc caccttttct tttgccgacg gcatcttaat cggatgtaaa ccattcattg
     1381 atgttttcct ctctctgtta ccactcgtgc ggcaagttcc tcataatacg gctccatctg
     1441 cgctgccgcc ccgcgcagtt cgtctgcact gagctgtctg ctttttactt cctgtttctg
     1501 ccagaagccg tcctgccatt ccgttccttt gcggtactct gcatctgcgc ttcccgtctc
     1561 ctgccgcggc aggctgatcg caaaacgcac atagcactcc accgcattct tcaggatatt
     1621 ccgcacataa gcattgtctg tcatcccata gattctgcgg tgaaattcca gttccgtcag
     1681 cgtctcctcc acaaaaatac cgggattctc tccctgctgc tccgatgctg caagatcgca
     1741 gagcacacgg cgttcaatat cgccgatgat accgtaaatc tcccggatat gtgcatctga
     1801 aaaatctgcc gtcaccacat gtctggtcgc aagacgcacc gcgagtccct gatgttcaat
     1861 gatctgcaac gcctcgcgca cagggattct cgatgcgccg agaagccccg agagttcctc
     1921 ctgcttcaat ggcgtaccgc cctggatcgc tcccgacaag atctgatcgg ccagctgatc
     1981 tgcgatataa tctgctgttc tcttgttcat ctgtatctcc atactgcagc cgtactcctc
     2041 tatcctctta acagaagttc tttctccaca actttgccat ttccgatata gatgcgcggc
     2101 acacgtttgc tcaccgcaca ggtaagctcg tatggaatcg tcccggcaag tgccgcaaga
     2161 tcattgatgg agttctgttt tccgaacacc tcaacctcac ttccaacatc cacacccatt
     2221 ttccctgtca gatcaatcat acacatgtcc atgcagatct tcccacgctg cggagccggt
     2281 ccctcttttg tcatcagcgc gcagcggttg gaaaggcagc ggaaaaagcc atccgcgtag
     2341 ccatagggaa ccacaccgat ccgcgtggtg tgctctgtct taaaaatgcc gccgtagctg
     2401 atcgccgtcc ctgccggata gatcttgatg gtgctgaccg tcgtctttaa cgtcatgacc
     2461 ggcaaaagcc ccagctcgtc cgcaaactcc ccgtagccgt acagaagcag tcccgggcgc
     2521 accatatcaa gaaaggtctc cggatatctt gcaaccgcgc cggtgttcgc gcagtgacgg
     2581 agcgcgaact ttcttcccag gcgcttttcg acttcctcaa tcacgcgcat aaacaggtcg
     2641 aactgatgct ccgtgtattt tttgcagtcc tcgccgggct cgtccgatac cgcgaaatgc
     2701 gtaaagatac cctccgcatc cagtcctggc agcgagcagc cgtgacagat tcccgccacg
     2761 cccgtctcaa aatgatctcc gtcgcacaga tatcccagac gcgacattcc ggtatccacc
     2821 ttgatgtgga tctttaagat gccgccgcac ttgaccgcct cttcgctgta ctcaagcgct
     2881 ttcgcctcac agctgaccgc ctgtgtaatg tgatactcga tcagcctcga cacctgctcc
     2941 ttcggtgtgt ggccgagaat taaaatcggc atcgtaacat cgtttaaacg cagctctacc
     3001 gcctcatcaa tgctgctgac cgccagataa tccgcaccaa gctcctgcag cagatgcgat
     3061 acctgtacag aaccgtgtcc gtaggcatcc gccttcacaa cccccagaaa ctttacatct
     3121 gcaccgattc tctctcttaa tttatgataa ttgtgtgcca gggcatccag atcaatctcc
     3181 gcccaggtac gtctcaatgt cgactccatg ccgcactgcc tcctgatgat ttcttctata
     3241 tttatacgaa tactataatc cacgcgccac atttatgcaa acaaaaagtt cccttccatg
     3301 ctttgccttg ataagtaata ttataagaaa cacacttggg gatttcatgt ggaaatacag
     3361 atggaatatt cggttcaaaa aatatggcat ttctaaccaa ccgaatggtt gcatcttcga
     3421 tgatccgtgt ctttccaagt ttggagcact ccacgggtca tgtccgcact ccggaagaca
     3481 atttgtagga cttttgtgtg tgtcccttat ggcacctctg aacaaaacat aaccgcgcga
     3541 agccgctgtc tgagtcggca tctgccctat tttcaatagc agatgccggc gagttcggtt
     3601 cgcgcggttt tatattgtct gtgaagaggt gccattaggg acaccagaaa gtccgcccat
     3661 tgtctccggt gtgtcggaca tgcacccgtc ggattgtcca aacgtggaag agacacggaa
     3721 tctgatgcaa acattcagcc ggaagaaatg cacgcatttt tttcaacgaa tgttccatct
     3781 acacttccag cgaaatcgaa aaatgtgatc ttttatccta gtacatctct ttcgacgcca
     3841 gctgtccgcc tgaagcggca gcctcccgtc tatttttcat catagagatg acattttacc
     3901 ttatgtccgt tgccgacatc cttgacctgc ggctcttcgt gtttacagat ctccatgcac
     3961 tcgctgcagc gggtgtggaa cttacatcct gcaggcggat tagccgggga aggaatatc
//
LOCUS       DBMDQJ010000150         4006 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8382_length_4006_cov_1.545198, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000150 DBMDQJ010000000
VERSION     DBMDQJ010000150.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8382_length_4006_cov_1.545198"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="ACZO9R_04200"
     CDS             complement(<1..920)
                     /locus_tag="ACZO9R_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HIR0912303.1"
                     /translation="MREELLQERKKAIYGLICDELYVPMKAKEIAAVLNVPKSDRRDL
                     AEVLDALVAEGKVEVSSKGKYMKSEGKFLTGVFAAHPRGFGFVTVEGEDEDIFIPESQ
                     VNGAFHGDTVQVALTRAEGHGRRREGQITKILTRGVSRIVCTYEKSKTFGFAVPDNPR
                     FGQDIFIPQERSKGAVSGHKVVVELTDFGGNGKKPEGKVVEILGHVNDPGTDILSIVR
                     GYDLPTEFSEKIMNQVERVAGEVSTADMAGRMDLRDWQTVTIDGEDAKDLDDAITLTK
                     EGDVYRLGVHIADVTNYVQERSALDVEALKR"
     gene            complement(972..1211)
                     /gene="secG"
                     /locus_tag="ACZO9R_04205"
     CDS             complement(972..1211)
                     /gene="secG"
                     /locus_tag="ACZO9R_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR0912304.1"
                     /translation="MAILKTILTVVFILICLALTVIILLQEGKSAGLGAIAGAADTYW
                     GRNKGRSMEGRLVTGTKILVALFIIIAAVLNLGIF"
     gene            complement(1288..2598)
                     /gene="eno"
                     /locus_tag="ACZO9R_04210"
     CDS             complement(1288..2598)
                     /gene="eno"
                     /locus_tag="ACZO9R_04210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR0912305.1"
                     /translation="MALTMKITEVHAREILDSRGNPTVEVEVTAETETTGRKTTARES
                     VPSGASTGRFEAVELRDGDREYFGLGTKKVVDHVNTKIREALLGMNVLDQALIDRRMV
                     ELDGTDNKGNLGANAILGVSLACAKTAAAALDMPLYRYIGGGNAKRLPVPMMNVINGG
                     VHAKNSLDFQEFMILPIGAKSYREALRMGAEVYHFLRQILNEEGYATAVGDEGGFAPD
                     LADAGEVFRYLGKAVEKAGYTVGKDVVYAMDAAASELYNEETGMYHFPGENGICRDAK
                     EMIALYEDLAKRFPLVSIEDGLQEDDWEGWQSLTGRLGDGMQLVGDDLFVTNPKRIKC
                     GIKLNAGNAVLIKVNQIGTLTEALDAIEMAQCAGYHTVISHRSGETEDAFIADLAVAV
                     NAGQIKTGAPCRAERTAKYNRLLRIEEELGETAQFSPAEPFGRA"
     gene            complement(2662..3393)
                     /locus_tag="ACZO9R_04215"
     CDS             complement(2662..3393)
                     /locus_tag="ACZO9R_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0912306.1"
                     /translation="MLRRVIEIEEEKCNGCGACANACHEGAISIVNGKAKLMRDDYCD
                     GLGDCLPTCPMDAIHFVEREAAAYDAAAVEENKRKKAAQQAIASQPLPCGCPGTQAKT
                     IRHEQQAVCPTAAEESAEELTSELTQWPVQIKLVPVRAPYFDGAKLLVAADCTAYAYA
                     NFHKKYIRNHITLIGCPKLDDVDYSEKLTQIIAQNDIKSITVVRMEVPCCGGIEQAVK
                     NALAASGKMIPWQVDIISTDGRILE"
     gene            3521..>4006
                     /locus_tag="ACZO9R_04220"
     CDS             3521..>4006
                     /locus_tag="ACZO9R_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR0912307.1"
                     /translation="MQTFQGYDALLQASPVFYDIAPEEIHAMLDCLSAKTLSYPKGSF
                     LFHYGDHLDSLGLVVEGCVDVMKEDFWGNTNLMTRCRPGDIFGESYAIRTNCALEVSL
                     YAEQNSTVLYLNVSKMMTLCSRTCEFHNQLTRNLLSVLAERNFQLGQKLEHMTKRTTR
                     EK"
BASE COUNT          863 a         1299 c          835 g         1009 t
ORIGIN      
        1 cgctttagag cctccacgtc aagggcgctg cgctcctgca cataattcgt gacatccgca
       61 atatgaacgc ccaggcggta gacatccccc tccttcgtca gggtgatggc atcgtccaaa
      121 tccttggcat cctctccgtc gatcgtcacc gtctgccagt cccgcagatc catgcggcct
      181 gccatatcgg ccgtgctgac ctcacctgcc acgcgctcca cctgattcat gatcttctcc
      241 gagaactcgg tcggcagatc gtaacctcgc acgatcgaga gaatatccgt ccccgggtcg
      301 ttcacatgtc ccagaatctc tacgaccttt ccctccggct tttttccatt gccgccgaaa
      361 tccgtcagtt ccaccacgac cttatgtccg ctcaccgcac ctttagagcg ctcctgcggg
      421 ataaaaatat cctgtccgaa tctcggatta tccggcactg cgaacccgaa tgtcttgctt
      481 ttttcatagg tacagacgat ccggctgacc ccgcgggtca aaatctttgt gatctgcccc
      541 tctctgcgcc gtccgtgccc ctcggcgcgt gtcagcgcca cctgcacggt atcgccgtga
      601 aaagcaccgt ttacctggga ttccggaata aaaatatcct catcctcccc ctcgaccgtc
      661 acaaagccaa atcctctcgg atgcgcggca aaaacgcctg tcaggaattt tccttccgac
      721 ttcatgtact tgcctttcga tgacacttcc acttttccct ccgccacaag cgcatccagc
      781 acttccgcga ggtctcttct gtcactcttt ggcacattta atactgccgc gatctccttc
      841 gccttcatcg gcacataaag ctcatcgcag atcagtccgt aaattgcttt ttttcgttcc
      901 tgtaaaagtt cttctctcat atccttcctc tccgcagcaa aaaagctgcc gcagatgcga
      961 cagcctcatt tctaaaagat tcccagattc aatacagcgg caatgatgat gaacagcgca
     1021 accagaatct tggttcccgt caccagtctg ccttccatgg aacgtccctt attccttccc
     1081 cagtacgtat ctgccgctcc ggcgatcgct ccgagtcctg cggactttcc ttcctgtaat
     1141 aagataatga ctgtcaacgc caaacaaatc agtatgaaaa caacggttaa aatcgtcttt
     1201 aaaattgcca aaataattcc ctcctacggt aatcaactca caactttagc tattatagca
     1261 tactcccccc gcgttttcaa ctgtttttta cgctctcccg aaaggctccg ccggagaaaa
     1321 ctgcgccgtc tcgccaagct cctcctcaat ccgcagaagc cggttatact ttgcagtgcg
     1381 ctccgccctg cacggcgcgc cggttttaat ctgccccgca ttcaccgcga ccgcaagatc
     1441 cgcgataaac gcatcctccg tctccccgga acggtgggag atcaccgtgt gataaccggc
     1501 acactgtgcc atctcgatgg cgtccaacgc ctccgtcagc gtgccgatct gattcacctt
     1561 tatcagcacc gcatttcctg catttaattt gatgccgcac ttaatccgct tgggatttgt
     1621 gacaaacagg tcatcgccga caagctgcat gccatctccc agacgtccgg tcagactctg
     1681 ccagccctcc cagtcgtctt cctgcagacc gtcctcgatc gacaccagtg gaaaacgctt
     1741 tgccagatct tcgtagagcg cgatcatctc ctttgcatcc ctgcagattc cattctcccc
     1801 cgggaaatgg tacattcccg tctcttcatt atataattca ctcgctgcgg catccatcgc
     1861 gtagaccaca tcttttccga ccgtatagcc cgccttttcc accgcttttc ccagataccg
     1921 gaacacctca ccggcatccg caagatccgg cgcaaagccg ccttcatcgc cgaccgcggt
     1981 cgcatatccc tcctcgttta aaatctggcg caggaaatga tatacctccg cgcccatgcg
     2041 cagcgcttcc cgatagcttt tggctccgat gggaaggatc ataaattcct gaaaatccag
     2101 tgaattcttc gcatggactc cgccgtttat gacattcatc atgggaaccg gcagccgctt
     2161 ggcgttgcct ccgccaatat agcggtaaag cggcatatcg agtgccgctg ccgccgtctt
     2221 cgcgcaggcg agcgacacgc ctaagatcgc attcgcccca agatttcctt tgttgtctgt
     2281 cccgtccagc tcgaccattc tgcggtcgat gagtgcctga tccaaaacat tcattccaag
     2341 aagcgcttcc cggatctttg tgttgacgtg atccaccacc ttcttcgtgc ccagtccgaa
     2401 atactccctg tcgccgtccc gaagctccac tgcctcaaat cttccggtgc tcgctcccga
     2461 cggaaccgat tcccgcgccg tagtctttct ccccgttgtc tccgtctcgg cggtcacctc
     2521 cacctccacg gtcggattgc cgcgggaatc cagaatctcc cgcgcgtgta cctctgtaat
     2581 tttcatagta agtgccataa aaaatcccct ttcgcgtaac atacttctgc atcatagtat
     2641 gtcccgcaaa aggggaaagt attactcgag aattcttccg tccgtagaga taatgtctac
     2701 ctgccacgga atcatcttac cgcttgccgc gagtgcattt ttcaccgcct gctcgattcc
     2761 tccgcagcac ggcacttcca tgcgaaccac ggtaatgctc ttaatatcgt tctgcgcaat
     2821 gatctgggtc agcttctccg aataatccac atcgtctaac ttcggacatc cgatcagcgt
     2881 gatgtgattg cggatatatt ttttgtggaa attcgcatac gcatacgctg tacagtctgc
     2941 agccaccaga agctttgctc cgtcaaaata cggggcgcgc accggaacca gcttgatctg
     3001 caccggccac tgtgtcagtt ccgacgtaag ttcttccgca ctctcctctg cggcggtcgg
     3061 acagactgcc tgctgctcat ggcggatcgt cttcgcctgg gttcccggac atccgcacgg
     3121 aagcggctgt gacgctatcg cctgctgcgc tgctttcttt ctcttattct cctcaactgc
     3181 cgcagcatca taagccgccg cctcacgctc cacaaaatga atcgcatcca tcggacaggt
     3241 cggcagacag tcgccaagtc cgtcgcagta atcgtcccgc atcagcttgg ccttgccgtt
     3301 tacaatactg atggctccct cgtggcaggc gtttgcacat gctccgcagc cgttgcactt
     3361 ttcttcctca atctcaatga ctcttcgtaa catcatatcc tccattctaa ctccggcacg
     3421 catgccggaa attctctctt gacttttctt ccacaggata ctacaatagc gtcaacattt
     3481 ctgttgtttt tacaacaaaa agtcgaaagg acgaaaaata atgcaaactt tccaaggata
     3541 cgatgccctc ttgcaggctt ctcccgtttt ttatgatatt gcacccgaag aaatccatgc
     3601 catgctcgac tgtctgtcgg caaagaccct ctcctatcca aagggcagct ttctctttca
     3661 ctacggcgac catctggatt ctctcggtct tgtggtagaa ggatgtgtgg atgtcatgaa
     3721 ggaggatttc tggggcaaca caaatctgat gacccgctgc cgccccggag acatcttcgg
     3781 agaatcttat gccatccgta caaactgtgc gctggaagtc agcctctacg ccgagcagaa
     3841 ttccactgtg ctctacttaa acgtctcaaa aatgatgacg ctctgctccc gcacatgtga
     3901 attccacaac cagctcacaa gaaatctgct ctccgttctc gccgagcgca atttccagct
     3961 cggacagaaa ttagagcaca tgaccaagcg gaccacccgt gaaaag
//
LOCUS       DBMDQJ010000151         3996 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8404_length_3996_cov_1.465725, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000151 DBMDQJ010000000
VERSION     DBMDQJ010000151.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8404_length_3996_cov_1.465725"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(218..391)
                     /locus_tag="ACZO9R_04225"
     CDS             complement(218..391)
                     /locus_tag="ACZO9R_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912308.1"
                     /translation="MYDADVLLLDEPTSNIDSLNEGMILRSLKQECADKTILMVSHRK
                     STMGIADDVLDEK"
     gene            complement(475..1128)
                     /locus_tag="ACZO9R_04230"
     CDS             complement(475..1128)
                     /locus_tag="ACZO9R_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912309.1"
                     /translation="MFYAHTISPIAVITSVVMTIFLGRIHPVFGVLALFFYVLVGAVI
                     PVVSGRIGAAGGQEYREAFGALNTCVLDNLYGLEETLQYGQQEARIAKMAEQTGHLET
                     VNGALKRGENLQRVITDGVILLAGVLTALVGGTLAENGSIPGSGAVIAVIALASSFGP
                     TAELSALSNNLNHTLASGNRVLDILEEKPLVEEVTGKEETCGRNIHCAHVEASDAVL"
     gene            complement(1209..2375)
                     /locus_tag="ACZO9R_04235"
     CDS             complement(1209..2375)
                     /locus_tag="ACZO9R_04235"
                     /inference="COORDINATES: protein motif:HMM:NF024878.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR0912310.1"
                     /translation="MKNAETKAKRIRENFWDTVNAPYEDRELALWMKIIFGFIWAISI
                     LNGILYGAPVSYLLAMGMMVALLAGNIAVCRRHYRRWIDVVTFMLLSIPIFYVYYHAS
                     IGYFSVLFPMLFSCGIVFILGIRNSFVINLFYLAAVILCFRFDLNASAEDIYGENVAL
                     RFPYLYVCFVFMAYLLMYSIQHYWVEKQRRQERLERRVREEKEKLRGMSMRVMNAMCR
                     ALGAKIPGEEEHCRQVAEYAKEIAKRLDLPEDMVSGAYQAGLLHEIGMIGIPDELIQR
                     RNLTDEEYGVFQTYVKMGYDMISELQVADTIAQAVYYHRENYDGSGYLAGKCGNEIPV
                     LAQILAVADYADRHLRWGESTEMVRGKIKERENTRFEPKCAGIMEQILKERSED"
     gene            complement(2379..3572)
                     /locus_tag="ACZO9R_04240"
     CDS             complement(2379..3572)
                     /locus_tag="ACZO9R_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024878.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR0912311.1"
                     /translation="MKLFRGAVDTIKRVYRYEKTTGMDVGALQGVMLIFAVVLFLIDI
                     ENFRLGKYVIGIVTLVVGTVSVTAVFVIRRFQKVVLVCRIAVFLFLLLAAAILYAGAN
                     DGFSLLWYLLLPVITLVPLGMPFGAPVCIGFGLMIMTFFWTPLADILRYDYPRDYRVF
                     YPIFYWGFCLMDVAMDIFYKNYQIRQRQNEENLENEVQEAVAGTKNLMIHSVTAISQL
                     IDAKDRYTREHSQRVAEYSRLIADYMRGGCASGEELELIYRSGLLHDIGKIAVPDAVL
                     NKPERLTDEEYEVMKKHTVWGREILSGLEFLPQADLGATYHHERYDGSGYPYGMHGEE
                     LPEMVRIISAADALDAMNSNRCYRKHCDRDYIIGEFRKGAGKQFDAGVAQAVVALIEE
                     GRITV"
BASE COUNT          964 a         1235 c          807 g          990 t
ORIGIN      
        1 cagcggcttc gcgcggttat gttttgttca gaggtgccat aaaggaacac acacgaaagt
       61 cctacaaatt gtctgccggc ttgcggacac agtccgtgga gtgctccaaa cttggaaaga
      121 cacggattat cacagatgcc atctgcggca tggttagaaa taccggattt tgcggtcaga
      181 atttgccgcc gcatgtttat ctgaaacttt taagtatcta tttctcgtcc aaaacgtcat
      241 ccgcaatgcc cattgtggac ttcctgtgcg acaccatcag aattgttttg tccgcacatt
      301 cctgctttaa gcttcgcaga atcataccct cgtttaagct gtcaatattg ctggtcggtt
      361 catccagaag cagaacatcc gcatcgtaca aaaaggctcg cgcgattccg aggcgctgcc
      421 gtattgatct cccctatctt tgtgccgtcg taggcgatct ccccggcatc cggctcatag
      481 aaccgcatca gaagcttcaa catgggcaca gtgtatgttc cgaccgcacg tctcctcttt
      541 tccggtcact tcctccacaa gcggtttttc ctccagaata tccagcacac ggtttccgct
      601 cgctaaggta tgatttagat tatttgacag cgccgagagt tctgccgtcg gtccgaaaga
      661 acttgcgagc gcgatcaccg caatcaccgc cccgcttccc gggatgctgc cgttttctgc
      721 aagcgttccg cccacgagcg ccgtaagcac acccgcaagg agaatcacac catcggtaat
      781 cacgcgctgt aagttctcgc cccgcttcaa tgcgccgttg actgtctcca gatgtccggt
      841 ctgctccgcc atcttagcga tgcgtgcctc ctgctgtccg tactgcagcg tctcctcaag
      901 accgtagaga ttgtccagca cacaggtatt caaagcgccg aacgcctccc ggtattcctg
      961 tccgccagca gctccgatcc gtccgctgac aaccggaatc acggctccga caagcacata
     1021 gaagaaaagt gccagcactc cgaaaacggg atgtatcctt cctaaaaaga tcgtcataac
     1081 caccgatgta atgaccgcga tcggcgagat cgtgtgcgca taaaacacct cttcacggat
     1141 ttttcctgca tctccttttc ccccgcacgc ttctgttcta tctcctgcgc cgtcattttc
     1201 tccattccct agtcctcact tctctccttt aaaatctgtt ccattatccc ggcgcacttt
     1261 ggttcaaacc ttgtattttc ccgctccttt atcttgcctc tcaccatttc cgtactctcg
     1321 ccccagcgta gatggcggtc cgcatagtcc gccaccgcga ggatctgcgc gagcaccggt
     1381 atctcattgc cgcactttcc cgccagataa ccgctgccat catagttctc ccgatgatag
     1441 tacacggcct gcgcgattgt atccgccacc tgcagttcgc tgatcatatc atagcccatc
     1501 ttcacataag tctggaacac accgtactcc tcatccgtca ggttccgtct ctggatcagt
     1561 tcgtctggaa tgccgatcat tccaatctcg tgcagaagac ctgcctgata agccccggac
     1621 accatatcct ccggaagatc cagtcttttt gcaatctctt ttgcgtattc cgccacctgc
     1681 ctgcagtgtt cctcctcacc gggaatcttc gcgcccagcg cgcggcacat cgcattcatc
     1741 acacgcatgg acattccccg cagcttttcc ttttcctcgc gcacgcgccg ctccagccgt
     1801 tcctgccgtc tctgcttctc cacccagtaa tgctggatgg agtacattaa cagatatgcc
     1861 atgaacacaa agcacacata cagataagga aaacgcagtg ccacattctc cccgtagata
     1921 tcttccgcgg aagcattcag gtcgaaccgg aagcacaaaa tcaccgccgc aagatagaac
     1981 agattgatga caaaactgtt gcggattcct aagataaata caattccgca ggaaaacagc
     2041 atcgggaaca atacactgaa gtacccaatg ctcgcgtggt aatacacata aaaaatcgga
     2101 atgctgagca acatgaatgt caccacatcg atccatctgc ggtaatgcct ccgacagacc
     2161 gcaatattgc cggcaagcaa tgccaccatc atccccattg caagcagata ggacaccggc
     2221 gcaccgtaaa gaatgccgtt taaaatactg attgcccaga tgaatccgaa tataattttc
     2281 atccagagtg ccaattccct gtcctcataa ggagcattga ccgtatccca gaagttctct
     2341 cttattctct ttgcttttgt ctctgcgttc ttcatccttt ataccgtaat cctgccttcc
     2401 tcgatcagtg caacaaccgc ctgcgccact ccggcgtcaa actgtttccc ggctcccttt
     2461 cggaattcgc cgataatgta atccctgtca cagtgctttc tgtaacagcg attggaattc
     2521 atcgcgtcta acgcatccgc cgcactgata atgcggacca tctccggaag ctcctccccg
     2581 tgcatgccat aaggatagcc gcttccgtcg taccgctcgt gatggtatgt cgcgcccaga
     2641 tccgcctgcg gcaaaaactc cagaccggat aagatctctc tgccccagac cgtatgtttt
     2701 ttcatcacct cgtactcttc atccgtcagc cgttccggct tatttaaaac ggcgtccggc
     2761 acagcgatct tgccaatgtc atgaagcaga ccgctgcggt agatcagttc cagttcctcc
     2821 cctgatgcgc agcctccccg catataatcc gcgatcagcc tggaatactc cgccacacgc
     2881 tgggagtgtt ctctcgtgta acggtccttg gcatcgatca gctggctgat cgccgtgact
     2941 gagtgaatca tcagattctt cgtgcccgca accgcctcct gcacctcatt ctccagattt
     3001 tcctcattct ggcgctgccg gatctgataa tttttgtaaa aaatatccat cgccacatcc
     3061 atcaggcaga atccccagta aaaaatcgga tagaacaccc gataatccct cggataatca
     3121 taccttaata tatccgcaag cggcgtccag aaaaaagtca taatcatcag accaaatccg
     3181 atgcacaccg gcgccccaaa tggcattcca agcggaacca gcgtgataac cggcagcagc
     3241 agataccaca gcagggaaaa gccgtcattc gcacccgcgt acagaatggc agccgccagc
     3301 agcaggaaca aaaagaccgc aatccggcac acgagaacca ccttctggaa ccgccggatt
     3361 acaaacaccg ccgtcacgct cacagttccc acgaccagtg tcacaattcc gatgacatat
     3421 tttcccagac ggaaattctc aatatcgatc agaaacaaca ccaccgcaaa gatcagcatg
     3481 accccctgca gtgccccaac atccatgccg gttgtcttct cataccggta cactcttttt
     3541 atcgtatcta ccgctccccg aaacagcttc atttgtctcc cgctcctttt atccgccgaa
     3601 ttagtttatc ctaactattt tcttcttgtc aagcatcatt ttcatatctt ttattctacc
     3661 aaatcccact tcacatgtac agcggagcat tttatggggc tgccgcaata agcatattac
     3721 gctttgctct gataagtaat attattagaa acagatattt aaaagttcca gataaacatg
     3781 cggcaacaaa ttctgaacgc aaaatccggt atttctaacc atgccgcaga tgacatctgt
     3841 gatgatccat gtctttccaa gtttggagca ctccacggac tgtgtccaca agccggcaga
     3901 caatttgtag gacttttgtg tgtgtccctt atggcacctc tgaacaaaac ataaccgcgc
     3961 gaagccgctg tctgagtcgg catctgccat attttc
//
LOCUS       DBMDQJ010000152         3995 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8407_length_3995_cov_1.253724, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000152 DBMDQJ010000000
VERSION     DBMDQJ010000152.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8407_length_3995_cov_1.253724"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="ACZO9R_04245"
     CDS             <1..458
                     /locus_tag="ACZO9R_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0912312.1"
                     /translation="MTVEEALEFFNVPSIHRKIQTLYDVGLSYVKLGQPSTELSGGEA
                     QRIKLATELSKRSTGKTIYILDEPTTGLHFADVHKLIEILRRLSDGGNTVVVIEHNLD
                     VIKTADYIIDMGPEGGDGGGTVIAQGTPEEVAQIPESYTGQYVKKYLEK"
     gene            1003..1998
                     /gene="mreB"
                     /locus_tag="ACZO9R_04250"
     CDS             1003..1998
                     /gene="mreB"
                     /locus_tag="ACZO9R_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007888000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HIR0912313.1"
                     /translation="MMSTDIGIDLGTASILVYIKGKGVVLKEPSVVAFDRDTNKIKAI
                     GEEARLMLGRTPGNIVAVRPLRQGVISDYTVTEKMIKYFIQKALGKKTFRKPRISVCV
                     PSGVTEVEKKAVEDATYSAGAREVAIIEEPIAAAIGAGIDISRPCGNMIVDIGGGTAD
                     IAVISLGGSVVSTSIKIAGDDFDEAIVRYMRKKHNLLIGERTAEDIKIKIGSCFPLAQ
                     NETMDVRGRNLVTGLPKTVTVSSEETEEALKEPTLQIVEAVHSVLEKTPPELAADVAD
                     RGIVLTGGGSLLRGLEELIEDKTGINTMTAEEPMTCVAVGTGKFVEFLSGKRDDD"
     gene            2118..2912
                     /locus_tag="ACZO9R_04255"
     CDS             2118..2912
                     /locus_tag="ACZO9R_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body protein"
                     /protein_id="HIR0912314.1"
                     /translation="MVKGLYTAYTGMINEQKRLDVLSNNLANADTNGYKKEGTTSQTF
                     ADELAIKIKDTSSYGLAQKLGTISMGVHIGETYTDYSTGSFKVTDNSTDFAIKGDGFF
                     AIAYTDKQGNSSVKYTRDGAFTVNTQGYLVTKDGDYVLNANDARNGNVNGRIQVDPTQ
                     KITVDELGNIYQNDQQVGTIGIVDFADYDYLAKYGENMYDLVNGGTVTAADGRIVQGT
                     LESSNVNVVSEMVNMITISRAYQAGQKVINTIDETLDKAVNQVGNV"
     gene            2930..3745
                     /gene="flgG"
                     /locus_tag="ACZO9R_04260"
     CDS             2930..3745
                     /gene="flgG"
                     /locus_tag="ACZO9R_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HIR0912315.1"
                     /translation="MMRALYTAATGMLAQQTNVDNISNNLSNVNTVGFKQEKAEFKSL
                     LYQTIQTKTTSANGEQKPIPAQVGLGTRVAAVTSVYTQGALQASENDTDFAIVGDGFF
                     AVRCADGETRYTRAGDFVWAVGTNGVTLTNPDGYPVLDSNNQQIVLPANISSSSVTVS
                     SDGRIGYTTANGTYVNMNRQIGLFQFNNPSGLEKTGDNLLEVTAASGAAMNENTTAGL
                     TRSQVRQNYLEASNVQVADEMVNMIIAQRAYQLNSKAITTSDEMLEMANNLKR"
     gene            3798..>3995
                     /locus_tag="ACZO9R_04265"
     CDS             3798..>3995
                     /locus_tag="ACZO9R_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912316.1"
                     /translation="MSFSIDGISSLVSNAQNSAAAGSASALKNSISSVSSGSSEEELL
                     SVCKDFESYFVEEVLKEVKENL"
BASE COUNT         1163 a          800 c         1170 g          862 t
ORIGIN      
        1 atatgacggt ggaggaggcg ctggagtttt ttaatgtgcc gtccatccac cgcaagattc
       61 agacgctcta cgatgtggga ctttcctatg taaaactggg acagccgtcc acagagcttt
      121 cgggaggaga ggcgcagcga atcaagctgg caaccgaact ctccaagcga agcaccggaa
      181 agacgatcta cattctggat gagccgacga ccggacttca ttttgcggat gtgcacaagc
      241 tgatcgagat tttgcggaga ttgtccgacg gcggcaatac cgtggtggtt attgagcaca
      301 atctggacgt gatcaagacg gcggattaca tcatcgacat gggaccggaa ggcggagacg
      361 gcggcggaac ggtcatcgcg cagggaacgc cggaagaggt ggcgcagata ccggagtcct
      421 acaccggaca gtatgtcaag aaatatctgg aaaaatagtg gaaataagga gaaggcactg
      481 gacgcaaacg tggaaaacac gcggcataca gtgccttttt tgacaacaga tgcgctggca
      541 tctgcggtca gagaaatgtt gtgaaatata tggcatcgaa aatttatatg gatgaaaatt
      601 cgttataccc ggggagtacc tcccggcgtt tggtgtttgc gccggaaggt atgagttatg
      661 aaaaattata ctaaccgcgg aatatttgag gaatatttcc ggggcggtgt atgtttggta
      721 caccatttag tacgtaaaaa aatataaccc gcaaatgtga tgaatacgtg atgaaaaaat
      781 gaaaaaatat gatattccgg acaaaaaatg aagaaattct tatttttttc aaaaatggcg
      841 gcgcgggcga aaaaaagctt tgaaaaatcc cagtttttgt atataataat gctgtatatg
      901 taaaaatatg attaaatgca tctttatgtc cttttggacg tgaagaaaga ttcaacgaat
      961 agatgaacca ttggcagaga aaagaaaggg gcagattaga atatgatgag tacggacatc
     1021 ggtatcgatc ttggtactgc aagtatcctg gtatatatta aaggcaaagg tgtagtgtta
     1081 aaggagccgt cagtagtggc ttttgatcgt gatacaaaca aaatcaaggc aatcggagag
     1141 gaagcgagac tgatgctcgg cagaacgccc ggcaacattg tagcagtccg tccgctgcgt
     1201 cagggtgtta tttctgatta tactgtcacc gagaagatga tcaagtattt catccagaag
     1261 gcacttggca agaagacatt ccgcaagccg cgcatcagcg tgtgtgtgcc gagcggagtg
     1321 accgaggtcg agaaaaaagc ggttgaggat gcgacctatt ctgcaggtgc gcgtgaggtg
     1381 gcgattatcg aggagccgat tgcggcagcg atcggagccg gaatcgatat ttcaagacca
     1441 tgcggcaaca tgatcgtgga tatcggcggc ggtaccgcgg acatcgccgt gatttctctg
     1501 gggggatcgg ttgtcagcac ttccatcaag atcgccggag atgattttga cgaggcgatt
     1561 gtccgttaca tgagaaagaa acacaatctg ttgatcggtg agcgcacagc ggaggacatc
     1621 aagatcaaga tcggttcctg tttcccactg gcacagaatg agaccatgga cgtcagaggc
     1681 cgtaacctgg taaccggtct gccgaagacg gtaaccgttt cttccgagga gacggaagag
     1741 gcgctgaagg agccgacact gcagatcgtg gaagcggtac actcggtgct ggagaagacg
     1801 ccgcctgagc ttgcggcgga cgtggcggac cgcggaatcg ttctcacggg aggcggatca
     1861 ctgcttcgcg gacttgagga actgatcgag gataagacgg gcatcaatac catgacagcg
     1921 gaggagccga tgacctgtgt ggcagtcgga accggaaagt tcgtggagtt cctgtccgga
     1981 aagcgcgacg acgattaagt ttgacggcag ttatgacgat ataaacaaca aagagccctt
     2041 gggatgcggg agcgtctgat ggattctggt gagaagcagg cagaagttac aagaggtaac
     2101 aggaaaagga gcagtctatg gtaaagggat tgtatacagc gtatacagga atgataaatg
     2161 aacagaagcg tctggatgtg ctttccaaca atcttgcaaa tgcagatacg aatggttata
     2221 agaaagaagg cacaacgtcc cagacatttg cagatgagct ggcaatcaag atcaaagata
     2281 catcgtctta cggtcttgcg cagaagctgg gtacaatcag catgggcgtt catatcggtg
     2341 agacgtatac ggattacagt accggaagtt tcaaggttac tgataacagt acggattttg
     2401 caatcaaggg agatggattt tttgcgattg cctacacgga taagcaggga aattcatccg
     2461 taaaatatac aagggacgga gcgttcacgg tcaacaccca gggctatctg gtgaccaagg
     2521 acggcgatta tgtcctgaat gcaaatgacg ccagaaacgg caatgtgaac ggacggattc
     2581 aggtggatcc gacccagaag atcaccgtgg atgagcttgg caatatctat cagaacgatc
     2641 agcaggttgg aacgatcggc atcgtcgatt ttgcggatta tgactatctg gcgaagtacg
     2701 gtgagaatat gtacgacctt gtgaacggag gaacggtgac ggcagcagac ggcaggatcg
     2761 tgcagggaac gctggagtcg tccaatgtga atgttgtttc tgagatggtc aatatgatta
     2821 cgatttccag agcatatcag gcaggacaga aggttattaa cacgattgat gagacactgg
     2881 ataaggcggt caatcaggtc ggaaatgtat agacggaggt agctgacata tgatgagggc
     2941 actttatact gcggcaaccg gtatgcttgc gcagcagacg aacgtggaca atatttcaaa
     3001 caatctttcc aatgtgaata cggttggatt caagcaggag aaggcagagt ttaagtcgct
     3061 gctttaccag accattcaga cgaagacaac gagtgcaaac ggagagcaga aaccgattcc
     3121 ggcacaggtg ggactcggaa cgagagttgc ggcagtcaca tccgtgtaca cacagggagc
     3181 gctgcaggcg agtgagaatg ataccgattt tgcaatcgtc ggagacggat tttttgcagt
     3241 gcgctgcgca gatggcgaga cacgctatac cagagcggga gattttgtgt gggcagtggg
     3301 aaccaacggc gttacactga ccaatccgga cggctatccg gtgctggaca gcaataacca
     3361 gcagatcgtt cttccggcca atataagttc cagcagtgtg acagtatcga gcgacggcag
     3421 aatcggttac acgacagcaa atggaactta tgtgaatatg aaccgtcaga tcggactgtt
     3481 ccagtttaac aacccgagcg gccttgagaa aacgggtgac aaccttctgg aagttacagc
     3541 agcttcgggt gcggcaatga atgagaatac gacagcaggt cttaccagaa gccaggttcg
     3601 ccagaattat ctggaggcgt ccaatgtaca ggtggcggac gagatggtca atatgattat
     3661 cgcacagcgt gcctatcagt tgaactccaa ggcaatcacg acatcggatg agatgctgga
     3721 gatggcgaac aacctgaaac gataagcaga tgataagcga acggtaagcg gatgataagc
     3781 ggaatggagc gtaaagtatg agtttttcga tagacggtat ttcatcattg gtcagcaatg
     3841 cacagaacag cgcagccgca ggcagcgcgt cggcgctgaa gaattcgatc agtagtgtgt
     3901 cctccggttc gagcgaggaa gaactgctct cggtctgcaa ggattttgag tcctactttg
     3961 tggaagaggt attaaaggaa gtaaaggaga atctc
//
LOCUS       DBMDQJ010000153         3988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8434_length_3988_cov_1.509390, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000153 DBMDQJ010000000
VERSION     DBMDQJ010000153.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3988
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8434_length_3988_cov_1.509390"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            204..1055
                     /locus_tag="ACZO9R_04270"
     CDS             204..1055
                     /locus_tag="ACZO9R_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HIR0912317.1"
                     /translation="MEFIKNVIRGITIGIANVVPGVSGGTIMVSLGIYDELLQAVTGF
                     FRHKKESILKLFPYLFGMGIGIICLSFMIGYLFENFPFQTAMLFIGLIFGGVPLIWRK
                     VSRGYPKVPELALFLGFFCLMIGMQFLGKGSEQVLAAGPGTAVKLFAVGLAAAATMVI
                     PGVSGSMLLMALGYYTPVIDHINAFVVAVVMLDAGTAWYCLVLLVPFGCGVLGGIFLV
                     AKLMEYLLCRFERLSYWAIFGLLAASPVAILGKIPLRGTSPAVIAWGVMLFSVGAVVT
                     GLLAGET"
     gene            1241..2134
                     /locus_tag="ACZO9R_04275"
     CDS             1241..2134
                     /locus_tag="ACZO9R_04275"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR0912318.1"
                     /translation="MKHTIIAIIAGWIILSMILIIFLLVRTRSMERKLDELWQMQRVM
                     NQQVPDTEALPQEETSGMDTETVAVTAPASGIDETDNLAQDGDVHKVYLTFDDGPSEN
                     TDAILDVLSEYDVKATFFVVGKEDEASAAIYQRIVDEGHTLGMHSYSNKYSLIYQSED
                     AFEADFHQLQDYLYEVTGEKSRYYRFPGGSSNQISNVPMSSLIRFLNSEDVVYYDWNV
                     SAGDAANAAYTPEELVENVVSDVSKYKTSVVLLHDSEDRSTTVEAVGPLIEALQDMDA
                     EILPIDEDTQVIQYVKADSAR"
     gene            2160..2987
                     /locus_tag="ACZO9R_04280"
     CDS             2160..2987
                     /locus_tag="ACZO9R_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HIR0912319.1"
                     /translation="MGFFKDFKDDFSQAVNELMPGEDNLESTDKDDDMVVNTLEDEVD
                     VASELSKLDGLLEQVTKQEPALERTQAATVEPQKTREEYTQHAMAAAQEEKKMADNTV
                     TPVQVAQTGAQAAAPQEKPIVSDEVSVITEGTIINGDVISNGSLDIRGQVDGNVSCNG
                     KLTVTGVVNGNSNTSEFFADSAQVEGEVVSSGTVKIGLGSVIIGNVTSNSAVIAGAIK
                     GDIDVQGPVVVDTSAVVMGNIKSRSVQINNGAVIEGFCSQCYADVDVQSLFNAKKGN"
     gene            2993..3691
                     /gene="pyrH"
                     /locus_tag="ACZO9R_04285"
     CDS             2993..3691
                     /gene="pyrH"
                     /locus_tag="ACZO9R_04285"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR0912320.1"
                     /translation="MKRVLLKLSGEALAGDKHTGFDEPTVTEVAKQVKQIVDGGTEVG
                     IVIGGGNFWRGRTSETIDRTKADQIGMLATIMNCIYVSEIFRSVGMQTQILTPFECGS
                     MTKLFSKDRANKYFAKGMVVFFAGGTGHPYFSTDTGIVLRAIEMEADGIYLAKAIDGV
                     YDSDPKLNPNAVKYDEVSIQEVIDKKLAVVDLTASIMCMENRMPMYVFGLNEENSIVK
                     AMTGEFSGTKVTVS"
     gene            3718..>3988
                     /locus_tag="ACZO9R_04290"
     CDS             3718..>3988
                     /locus_tag="ACZO9R_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043023 - ribosomal large subunit binding
                     [Evidence IEA];
                     GO_process: GO:0002184 - cytoplasmic translational
                     termination [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HIR0912321.1"
                     /translation="MDQRLAVFDEKMGKTMNNLAEEFGGIRAGRANPHVLDKLRVDYY
                     GTPTAIQQVANVSVPEPRMIQIQPWEASMVKEIEKAILTSDLGINP"
BASE COUNT         1063 a          783 c         1190 g          952 t
ORIGIN      
        1 acaaggaccc cgcacgggca gcagccaagg cacttacgct gctggcgctg cagcaccggg
       61 ataggaaaat tgggaaagcc atcggggagc tcatcggccg gaaatctgct ggcagggatg
      121 gcagagcatc ttcttccgga taagaagagc gaatcgcttg cggcggaata gagaaaggca
      181 gcatgcaaaa aagtggagga tgtatggaat tcataaagaa tgtcataaga ggaattacga
      241 tcggaatcgc caatgtagtt ccaggagtga gcggcggtac gatcatggtg tcgctgggga
      301 tttatgatga acttctgcag gcggtcaccg gatttttccg tcataaaaag gaaagcatct
      361 taaagctgtt tccgtatctg tttggaatgg ggatcggaat tatctgcctt tcctttatga
      421 tcggttatct ttttgagaat tttccgttcc agaccgcgat gctttttatc ggactgattt
      481 tcggcggcgt gccgctcatc tggcgcaagg tgtccagggg atatccgaag gtgccggagc
      541 tggcgctgtt tttgggattc ttctgcctga tgattggaat gcagttcctt ggaaaaggca
      601 gcgaacaggt tcttgccgcc ggtcctggga cggcagtgaa gctttttgcg gtggggctgg
      661 ctgccgcggc tacgatggtc attccggggg tgagcggttc gatgcttttg atggcgctcg
      721 gatattacac gccggtcatt gaccatatca atgcgttcgt tgtggcggtg gtcatgctgg
      781 atgccgggac ggcgtggtac tgtcttgtcc tattagtccc gttcggatgc ggcgtgcttg
      841 gaggtatttt tcttgtcgca aagctgatgg aatatctgct ctgccggttt gagcggctga
      901 gctactgggc aattttcggg ctgctggcgg cgtccccggt tgccatatta gggaaaatcc
      961 cgctgcgcgg gacatcgccg gctgtcatcg cctggggcgt gatgctgttt agtgtggggg
     1021 cggtggtcac cgggctttta gcgggagaaa cataaaaatt cagaaaatac atgaaaaagt
     1081 gtagaaaaat catacgattt ttttgcgctt ttttctttga aaaatacaaa aatgtgatac
     1141 aatagataaa gtattatgat taacttgggg gttctacatg caggataagg attctgaggc
     1201 actccgcaat caacgcaggc ggagaaagcg gattgcaaga atgaaacata ccataatcgc
     1261 gataattgcc gggtggatca tcctgtccat gattctgatc atttttctgc ttgtccggac
     1321 gagatccatg gagagaaagt tagatgaact ctggcagatg cagcgggtga tgaaccagca
     1381 ggtgccggat acggaggcac taccccagga agagaccagc ggcatggata cggagacagt
     1441 ggctgtgacg gcacctgcat ccgggatcga cgagacagac aatctggcac aggatggaga
     1501 tgtacataaa gtttatctga cgtttgatga cgggccgtct gagaatacgg acgccatttt
     1561 agatgtgctg tcggagtacg atgtcaaggc aacctttttt gtggtcggaa aagaggatga
     1621 ggcatcggca gccatctacc agaggatcgt ggatgagggg catacgctgg gaatgcattc
     1681 ctattctaat aagtacagtc tgatctatca gtcggaggat gcgttcgagg cggattttca
     1741 tcagctgcag gattatctct atgaggtgac cggtgaaaag agccgctatt accggtttcc
     1801 gggcgggagc agcaatcaga tcagcaatgt gccgatgagt tcgctgatcc ggtttttaaa
     1861 cagcgaggac gtcgtttatt acgactggaa cgtctcggca ggtgatgcgg caaatgctgc
     1921 atatacaccg gaagaactgg tcgagaatgt tgtttcagat gtttcaaaat acaagacctc
     1981 ggttgtgctt ttgcacgact cggaagatag gtcaacaacg gtagaagcgg taggaccgct
     2041 gatcgaggca ttgcaggata tggatgccga gattttaccg attgatgaag atacacaggt
     2101 tatccagtat gtcaaagccg acagtgccag atgaccagat tataaatgga ggtaagaaca
     2161 tgggattttt caaggacttt aaggatgatt tctcacaggc agtgaatgaa cttatgccag
     2221 gagaagacaa tctcgaaagc acggacaagg atgatgacat ggtcgtgaac acactggaag
     2281 atgaagtgga tgtggcgagc gaattgtcga agctggatgg tctgttagag caggtaacga
     2341 agcaggagcc agctttggag cggacacagg cagcaacggt ggaaccacag aaaacaagag
     2401 aagaatacac acagcatgca atggcagcag cacaggagga aaagaaaatg gctgataata
     2461 cagtaacacc agtacaggta gcacagacag gagcacaggc ggcagcaccg caggagaagc
     2521 cgatcgtatc ggatgaggtt tccgtgatta cagagggaac gattatcaac ggtgatgtaa
     2581 tttcaaacgg atctctcgac atcagaggcc aggtagacgg aaatgtaagc tgcaatggaa
     2641 agcttaccgt aaccggcgtt gtcaacggta acagcaacac ttccgaattc tttgcggatt
     2701 ctgcacaggt agagggtgaa gtcgtaagct ccggtacggt taagattggt cttggttctg
     2761 taattatagg taatgtaaca tccaactcgg cagtgatcgc aggagccatc aagggtgaca
     2821 tcgatgtgca gggaccggtt gttgtggata cttccgcagt ggttatgggt aacatcaagt
     2881 cccgttctgt tcagatcaac aacggcgctg ttattgaggg attctgctcc cagtgctatg
     2941 cagatgtgga tgttcagagc ctgttcaatg ccaagaaagg aaactaagca tcatgaagag
     3001 agttctgtta aagttaagtg gtgaggcgct tgcaggtgat aagcacacag gatttgacga
     3061 gccgacagtc acagaggttg caaaacaggt aaagcagatt gtggatggtg gtaccgaagt
     3121 aggcattgtg atcggcggcg gcaacttctg gagaggacgc accagcgaga cgatcgaccg
     3181 caccaaagcg gatcagatcg gcatgcttgc aacgatcatg aactgcatct atgtttcaga
     3241 gattttccgc tctgtgggca tgcagacaca gattctgaca ccttttgagt gtggaagcat
     3301 gacaaagctg ttttccaagg atcgtgcgaa taagtatttt gcaaagggaa tggttgtgtt
     3361 ttttgcaggg ggaacgggtc atccatactt ttcgacagat acgggaattg ttctgcgtgc
     3421 gattgagatg gaggcggacg gaatctacct cgcaaaagcg attgacggcg tgtatgacag
     3481 tgatccgaag ctgaatccga atgcagtgaa gtatgatgaa gtctctattc aggaagtaat
     3541 tgacaagaag cttgcggtgg tggatctgac agcctccatt atgtgtatgg agaaccggat
     3601 gccgatgtat gtattcggac tgaatgaaga gaatagtatc gtaaaagcca tgacgggaga
     3661 gtttagcggc acgaaggtta cggtttctta gaattgatag aaaggcatgg tatatatatg
     3721 gatcagagat tagcggtatt tgatgaaaag atgggcaaaa caatgaataa tcttgcggag
     3781 gagttcggcg gaatccgcgc gggacgcgcc aatccgcatg tgcttgacaa gttgagagtg
     3841 gattactacg gtactccgac agcaattcag caggtggcga atgtcagtgt tccggagccg
     3901 cgtatgattc agattcagcc gtgggaggca tccatggtaa aggagattga gaaggcgatc
     3961 cttacttccg atcttggaat caatccta
//
LOCUS       DBMDQJ010000154         3975 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8468_length_3975_cov_1.552142, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000154 DBMDQJ010000000
VERSION     DBMDQJ010000154.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8468_length_3975_cov_1.552142"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            49..651
                     /locus_tag="ACZO9R_04295"
     CDS             49..651
                     /locus_tag="ACZO9R_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0912322.1"
                     /translation="MIHSVVPLVQCSEYNEHADYPYVSIDDYSAATSAAEYIVSCNCN
                     KIAMINGPMSFKYAVKRQEGFLDTLRKKDISVRPDWIVSLPEVNYQMAYSAVCRLLNN
                     EIRPNAFFVISDVFAAAVIRAAKRFDLRIPEDIIVVGFDNLDISIMTCPTITTVNQPS
                     FQLGYSACELLLDSINHPDMFPKSLILDTELIIRESSAKI"
     gene            complement(697..1401)
                     /locus_tag="ACZO9R_04300"
     CDS             complement(697..1401)
                     /locus_tag="ACZO9R_04300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:1902358 - sulfate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR0912323.1"
                     /translation="MYESYNALFAEHWKEQAGQDVTVIQSHGGSGKQALEVANGLEAD
                     VVTLALEGDVQVVADAGMIEDGFEDEFDQESSPYTSTIVFLVRKDNPKNIADWDDLLR
                     EDVGVITPNPKTSGGARWNYLAAWYYFESQGQSGEEITENMKTLYHNVLVLDSGARGA
                     TTTFAENFYRPSDPDVFSDYISTSGERVITELPADGKWIVDDIALTDIAHFGGWSEAS
                     AKHFADGGVFEAIHEQ"
     gene            complement(1440..2363)
                     /locus_tag="ACZO9R_04305"
     CDS             complement(1440..2363)
                     /locus_tag="ACZO9R_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR0912324.1"
                     /translation="MKKERLTKRLALFTALTLTGAMLAGCGNTDAAESSSDAQTGVTA
                     DTQVAAGDASADVVYRTLDEIKESGTINIGVFSDKNPFGYVDENGEYQGYDVYFANRL
                     AEDLGVDVNFVSTEAASRIEYLQTGKVDVILANFTVTPERAEEVDFALPYMNVSLGVI
                     SPDSAVVESLDDLTADDPVIVISGTTAETYLTENYPDLTLQKYDSYAAAKTALENGNG
                     VAWANDNTEVIAFALQNPGYTVGIPELGSTDTIAPAVSKGNTTVLDWINEEIKALGEE
                     NFFHADYEATLADTYGSDYEDTLVVEGGETK"
     gene            complement(2431..3192)
                     /locus_tag="ACZO9R_04310"
     CDS             complement(2431..3192)
                     /locus_tag="ACZO9R_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR0912325.1"
                     /translation="MKAEAEELLKIEHIKKSYEGNEILKDLSLTIHKGEVVVIIGPSG
                     CGKSTLLRCINALEPIQEGSITLRGETIDGKAKNIAGIRQKVGMVFQSYELFPHKTIL
                     ENVILAPLKVQKRTKEDAVSEAKQLLEKVGLLDKCDSFPRQLSGGQKQRVAIVRALMM
                     HPEIMLFDEVTAALDPEMVREVLDTMLELAKAGSTMVIVTHEMSFARAVADRVVFIDQ
                     GEIVEEGKPDEFFTHPTTERAKKFLHTFEFEAVRD"
     gene            complement(3194..3871)
                     /locus_tag="ACZO9R_04315"
     CDS             complement(3194..3871)
                     /locus_tag="ACZO9R_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR0912326.1"
                     /translation="MPDLGISVLLKGKNMIRLLGGLWVALRISLISVVISIPLGILLG
                     IVMTWKNPVTKAVTRVYLEIVRIMPQMVLLFLVFFGTTRMFGWNLSAEFASVIVFTFW
                     GTAEMGDLVRGALISIPKHQYESGAALGMTRSQIYFYIIIPQTLRRLIPLSINLVTRM
                     IKTTSLVMMIGVVEVLKVTQQIIEANRMASPNAAFGLYGTVFILYFLACWPISMLAGH
                     LEKKWSN"
     gene            complement(3852..>3975)
                     /locus_tag="ACZO9R_04320"
     CDS             complement(3852..>3975)
                     /locus_tag="ACZO9R_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR0912327.1"
                     /translation="YYKTAECLFLLVVFYLIILLPVSILGTLLERRLRYAGFGN"
BASE COUNT          924 a         1160 c          824 g         1067 t
ORIGIN      
        1 gccgccggag tcattttatt gaatcatgtc tccaaggaac agctggatat gatccactct
       61 gtcgtaccgt tagttcaatg cagcgaatat aacgagcacg cggattaccc ttatgtcagc
      121 atagacgact atagtgccgc aacaagcgct gcagaatata ttgtttcctg taattgtaat
      181 aaaatcgcaa tgataaacgg tcccatgtcc tttaaatatg ctgtgaagcg tcaggaaggt
      241 tttcttgata cgctccgcaa aaaagatatt tccgttcgtc cggactggat tgtcagcctg
      301 cctgaagtaa attaccagat ggcatactcc gcagtctgcc gtctgttgaa taatgaaatt
      361 cgtccaaatg cttttttcgt tatctcagac gtttttgccg ccgctgtcat tcgtgccgct
      421 aaacgctttg atcttcgtat tccggaggat attattgtcg tcggatttga taacctggac
      481 atctccatca tgacctgtcc gacgattacc actgtaaacc agccgagttt tcagctgggc
      541 tattcggcat gcgaacttct tcttgattcc atcaaccatc ctgatatgtt tcccaaatct
      601 ctgattctgg atacagaact tatcatccgg gaatccagcg caaaaattta acgcccggca
      661 gattgctctg ccgggcgttc cctgtaaatt tcctaatcac tgttcatgaa ttgcttcaaa
      721 cactccgccg tccgcgaaat gcttcgccga cgcctcactc catccaccaa agtgcgcaat
      781 atccgtgagt gcaatgtcat ccacgatcca ttttccgtct gccggcagtt ccgtgatgac
      841 gcgctccccc gatgtactga tataatcgga aaaaacatcc ggatccgacg ggcggtagaa
      901 gttctccgca aacgtggtcg tcgcaccgcg cgcacccgaa tccagcacca gcacattgtg
      961 atagagtgtc ttcatattct ctgtgatctc ttccccgctc tgtccctgac tttcaaaata
     1021 gtaccatgcc gccagataat tccagcgcgc tccgccggac gtcttcgggt tcggggtgat
     1081 gacgcccacg tcctcccgga gcagatcatc ccagtccgca atgttcttcg gattgtcctt
     1141 gcgcaccaga aaaacaatcg tcgatgtgta cggggaactt tcctgatcaa attcatcctc
     1201 aaaaccatcc tcaatcattc ccgcatccgc aaccacctgc acatccccct ccagtgccag
     1261 cgtcaccaca tccgcctcca ggccgtttgc cacctcaagt gcctgcttgc cggaacctcc
     1321 gtgcgactgg atcaccgtca catcctgccc tgcctgctcc ttccagtgtt ccgcaaacag
     1381 tgcattgtac gattcataca gttccctgtg ctttgaacct tccgttacat ggtcagttct
     1441 tacttggtct cgccgccctc taccacaagc gtatcctcgt agtcactgcc gtaagtgtca
     1501 gccagtgttg cctcgtaatc tgcgtggaaa aaattctcct cgccaagtgc cttgatctcc
     1561 tcattgatcc agtctaatac ggtcgtgttg cccttgctta ctgccggtgc aatcgtatcc
     1621 gtgcttccaa gctccggaat acctacggta tagcccgggt tctgaagtgc gaatgcaata
     1681 acctctgtgt tatcgtttgc ccatgcaaca ccgtttccgt tctcaagcgc agtctttgct
     1741 gccgcatagg aatcatattt ctgtaaggtc agatccggat agttctcggt caggtaggtc
     1801 tctgctgttg taccggaaat cacgatcacc ggatcatccg ctgtcagatc atccagactc
     1861 tcaaccactg cagaatccgg agaaatcaca ccgagggata cgttcatgta cggcagtgcg
     1921 aaatcaactt cctccgcgcg ctccggtgtc acggtaaagt tagccagaat gacatctact
     1981 tttccggtct gtaaatactc aatacggctt gcagcctcgg tggaaacgaa gttcacatcc
     2041 acgccgagat cctctgccag acggtttgca aagtagacat catatccctg atactctccg
     2101 ttctcatcca catagccaaa tggattcttg tcggaaaata caccgatgtt gattgtgccg
     2161 ctctccttga tctcgtctaa ggtgcggtat accacatctg cggatgcatc gcctgcggct
     2221 acctgtgtat ctgcggtaac tcctgtctgc gcatcactgc tgctctccgc tgcatctgta
     2281 ttgccacagc ctgccagcat tgctccagtt aatgtcagtg ctgtaaacag tgccagtctc
     2341 ttcgttaatc tttctttttt cataatattg tcctctcttt ctattccaaa cttccatcga
     2401 agcttcagaa tcaacctttt gtgccgctct ctagtcgcgg actgcctcaa attcaaatgt
     2461 gtgcagaaac tttttcgcac gctctgtcgt cggatgggta aaaaattcat ccggctttcc
     2521 ttcttccaca atctctccct ggtcaataaa gaccacacgg tctgcaaccg cccttgcaaa
     2581 ggacatctcg tgtgtgacga tcaccatagt gcttcctgcc ttagcaagct ctaacatcgt
     2641 atccagtacc tcgcgcacca tctccggatc aagcgctgcg gtcacctcat cgaacagcat
     2701 aatctcgggg tgcatcatca gtgcacgcac gatcgcaaca cgctgcttct gaccgccgga
     2761 aagctgtctc ggaaaactgt cacacttgtc gagaagccct actttttcca gaagctgttt
     2821 tgcctcggaa acggcgtcct cttttgttct cttctgcacc ttaagcggtg ccaggatcac
     2881 attttccagg atcgtcttgt gtggaaaaag ctcgtagctc tgaaatacca tcccgacctt
     2941 ctgacggatc ccggcgatat tcttcgcctt tccgtcgatg gtctcgcctc ggagcgtaat
     3001 gcttccctcc tgaatcggct ctaaggcgtt gatacatcga agcagtgtgc tcttaccgca
     3061 tccggatggt ccgatgataa ccacaacctc tcctttgtgg atggtcaggc ttagatcctt
     3121 taagatctcg tttccctcgt aggatttttt gatatgttca atttttaaca attcttctgc
     3181 ttctgccttc atatcagtta ctccatttct tttccagatg tcccgccagc atactgatcg
     3241 gccagcatgc cagaaaatac agtataaaca cggttccgta cagtccgaac gccgcattcg
     3301 gcgatgccat acggtttgcc tcgatgatct gctgtgtcac ctttaacacc tccaccacac
     3361 cgatcatcat cacaaggctt gtcgtcttga tcatacgggt gaccaggttg atcgacagcg
     3421 ggatcagacg ccgcagggtc tgcgggataa taatgtaaaa ataaatctga cttctcgtca
     3481 ttccaagcgc tgcaccactc tcatactggt gcttcgggat actgattaac gcgccgcgca
     3541 ccagatctcc catctctgcg gttccccaga atgtaaatac gattaccgat gcaaactccg
     3601 ccgataaatt ccagccaaac atccgcgtcg ttccgaaaaa cacgagaaac agcagtacca
     3661 tctgcggcat gatgcggaca atctccagat acactcttgt cactgctttt gtcaccggat
     3721 tcttccatgt catgacgatg ccgagcagaa taccgagcgg aatactgatg accacggaga
     3781 tcaagctgat cctaagggct acccacagac cgcccagaag tcttatcatg ttcttgccct
     3841 taagcaggac actaattccc aaatccggca tagcgcagcc tcctctctaa caacgttcca
     3901 aggatggata ccggaagtaa gataatcaga taaaatacca ccagtaagaa cagacactcc
     3961 gctgtcttat agtaa
//
LOCUS       DBMDQJ010000155         3975 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8469_length_3975_cov_1.439339, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000155 DBMDQJ010000000
VERSION     DBMDQJ010000155.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3975)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8469_length_3975_cov_1.439339"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            280..2328
                     /gene="ftsH"
                     /locus_tag="ACZO9R_04325"
     CDS             280..2328
                     /gene="ftsH"
                     /locus_tag="ACZO9R_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR0912328.1"
                     /translation="MDNQGSNNNYNNGNSNGGNNGDGGKGNHNGQILMAFILISLIAL
                     FIMSLVTNQFNQMSTQEVSYSEFLDMVNGTGKWEGRSVKSVEIGSYQIDITLNSDEKT
                     PYEVTYYCGRVADDELIPLLKEKGVDISGVIPDNTSTWIYSILSYLIPLVLIWVVLGV
                     VMRRMGGGAMGVGKSNAKVYVEKQTGVTFKDVAGQDEAKESLQEVVDFLHNPKKYRDI
                     GAKLPKGALLVGPPGTGKTLLAKAVAGEAKVPFFSLAGSDFVEMFVGVGASRVRDLFK
                     EAQKQAPCIIFIDEIDAIGKSRDTRYGGNDEREQTLNQLLAEMDGFDTSKGILILAAT
                     NRPEVLDKALLRPGRFDRRIIVDKPDLKGRLETLKVHSKDVMMDETVDLDALALATAG
                     LVGSDLANMINEAAINAVKNGRKFVNQSDLFDAFELVAVGGKEKKDRVMSDKERKIVS
                     YHEVGHAMVTALQKNTEPVQKITIVPRTMGALGYTLQTPEEEKYLQTRDELLAKITTY
                     MAGRAAEVLVFQSATSGAANDIEQATAIARAMVTQYGMSDKFGMMCLATVENQYLDNR
                     AGLICGEDTAAQIDKEVLAIINHAYDEAMRLLTENREVLDHIAEYLYEHETITGKEFM
                     KIFRELKGIPEPEDEAEKKTFFEQAEEARQELEEGKTAAESQNMDDVLLRNTQDHEEQ
                     "
     gene            2328..>3975
                     /gene="uvrC"
                     /locus_tag="ACZO9R_04330"
     CDS             2328..>3975
                     /gene="uvrC"
                     /locus_tag="ACZO9R_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR0912329.1"
                     /translation="MFVIEEELKKLPDQPGVYIMHDSRDAIIYIGKAVSLRKRVHQYF
                     QPSHDEGIKKAQMVKQIARFEYIVTDSELEALVLECNLIKEHRPKYNTMLRDDKTYPY
                     IRVTLGEDFPRVLFSRQQKKDKSRYFGPYTSAGAVKDTIELVNKIYQLRTCNRNLPRD
                     TGKDRPCLNYHIHQCTAPCQGYITKEAYRERVDAVVEFLNGNYAPVLKSLEEKMNTAS
                     ANLEFEKAIEYRELLNSVRQIAQKQKITHTDGEDKDIIALAADDRDAVVQVFFIRDGK
                     LIGRDHFYVKIGTEDTKAQILTTFIKQFYSGTPFIPREIMLPQEIEEQEVLADWLGEK
                     RGSKVYIRVPQKGMKEKLVELAQKNAKMVLAQDREKIKREEGRTIGALKEIEQLLDMK
                     GLNRVEAYDISNTSGFESVGSMIVYEKGKPKRSDYRKFKLRTVSGPDDYASMYEVLTR
                     RFTHGMREMEEMEEKDLSEEYGSFTRFPDLIMMDGGRGQVNIALKVLEELHLNIPVCG
                     MVKDDNHRTRGLYYHNVEIPIDRGSEGFKLITRIHRACRLP"
BASE COUNT         1086 a          850 c         1200 g          839 t
ORIGIN      
        1 gggttttcct cctcatataa aagtacagtt gtgtgaataa tagtaatacg atctatataa
       61 cccatacagt atacaactaa gtgcccggaa aatccagcac aatttcaaaa acacagaaaa
      121 tagtatgccg ggaaatttta gaattatttt agaaatgaca ggaaaacttc acgaagaacg
      181 tattgctttt aaattccatt ttcaatataa tatagaagat atacgataag aatccagact
      241 ctgaactggc ggagcctgtt ggaaaggata aatacttcaa tggacaatca gggatcaaat
      301 aacaattaca ataacggtaa ttcaaacgga gggaataacg gagacggcgg caaagggaat
      361 cacaatggtc agattttgat ggcatttata ctgatttcac tgattgcgct gtttattatg
      421 agccttgtaa ccaatcagtt caaccagatg agcacgcagg aggtgtctta ctcggagttc
      481 ctcgatatgg tgaacggcac cggcaaatgg gaaggcagaa gcgtgaagtc ggtggagatc
      541 ggttcgtacc agatcgacat cacactgaat tccgatgaga aaacgcctta cgaggtgact
      601 tactattgcg ggcgtgtggc ggatgatgag ctgattccgt tacttaagga aaagggcgtg
      661 gatatcagcg gagtgattcc ggataacaca tccacctgga tttacagtat tttaagctac
      721 ctgattccgc tggtgctgat ctgggttgtg ctcggcgtgg tgatgcgccg gatgggcggc
      781 ggcgccatgg gcgtcggcaa gagcaacgcc aaggtttatg tggagaagca gaccggtgtg
      841 acgttcaagg atgttgcggg acaggatgag gcgaaggagt cgctgcagga ggttgtcgat
      901 ttcctgcaca acccgaaaaa gtaccgcgac atcggtgcga agctgccgaa gggtgcgctt
      961 cttgtgggac ctccgggaac cggtaagacg ctccttgcca aggcagtggc gggcgaggcg
     1021 aaggtaccgt ttttctcgct ggcaggttcg gattttgtgg agatgttcgt cggtgtcgga
     1081 gcttcccgtg tgagggatct gttcaaggaa gcacagaagc aggcaccgtg tatcatcttt
     1141 atcgatgaga tcgatgcgat cggtaagagc cgtgacaccc gttacggcgg caacgacgag
     1201 agagagcaga cattgaacca gctccttgcg gagatggacg gttttgacac gtccaaggga
     1261 attctgattc tcgctgcaac caaccgtccg gaggtactgg acaaggcgtt gctccgtccg
     1321 ggacgttttg accgccggat cattgtcgac aagccggatc tgaaggggcg tctggagaca
     1381 ctcaaggtac attccaagga cgtcatgatg gatgagaccg tggatctgga tgcactggca
     1441 cttgcgacgg caggtctggt gggttccgat cttgccaata tgatcaatga ggcagcaatc
     1501 aatgccgtca agaacggcag aaagtttgtc aaccagtccg atctgtttga cgcgtttgaa
     1561 cttgtggcag tcggcggcaa ggagaagaag gatcgcgtga tgagtgacaa ggagcgcaag
     1621 atcgtgtcct accatgaggt cggccacgcg atggtgacgg cgcttcagaa gaacaccgag
     1681 ccggtacaga agatcacgat tgttccgcgt acgatgggag cgctcggcta cacgctgcag
     1741 acgccggagg aggagaagta tctccagacc agggatgaac ttttagcgaa gattaccact
     1801 tacatggcag gacgtgcggc ggaggtgctg gtattccagt cggcaaccag cggtgcggcg
     1861 aacgatattg agcaggcgac ggcgattgcc agggcaatgg taacgcagta cggtatgtcc
     1921 gataagttcg gtatgatgtg cctggcgacg gtggagaacc agtatctgga caaccgcgca
     1981 ggactgatct gcggtgagga cacggcggcg cagattgata aggaggtact tgccattatc
     2041 aatcacgcat atgatgaggc gatgcgtctc ctcacggaga accgggaggt gctcgatcat
     2101 atcgcagaat acctctacga gcatgagacg attaccggta aggaattcat gaagattttc
     2161 cgtgaactga aaggaatccc ggagccggag gacgaggcgg agaagaagac gttcttcgag
     2221 caggcggagg aagcccgtca ggaactcgag gagggaaaaa ctgccgcaga gagtcagaac
     2281 atggatgatg tattgctgcg caatacgcag gatcatgagg aacagtaatg tttgtaattg
     2341 aagaagaact taaaaaactc ccggatcagc cgggagttta tattatgcat gacagccgtg
     2401 atgccattat ctacattggc aaggcggtca gtctgcgcaa gcgggtgcac cagtattttc
     2461 agccaagcca tgatgagggt atcaaaaaag cgcagatggt aaaacagatt gcgcggtttg
     2521 agtacattgt gacggattcc gaactggagg cgctggtact ggaatgtaat ctgatcaaag
     2581 aacatcggcc gaagtacaac accatgctgc gggacgataa gacatatccg tacatcagag
     2641 tgactctcgg ggaagatttt ccgagagttc tcttttcccg ccagcagaaa aaagacaaat
     2701 cgcggtactt tggcccttac acgagcgcgg gagcagtcaa ggatacgatc gagctggtga
     2761 acaagatcta tcagctgcgc acctgcaacc ggaacctgcc gcgcgacacg gggaaggacc
     2821 gcccgtgcct gaattatcac attcatcagt gcacggcgcc ctgccaggga tatatcacaa
     2881 aggaagcgta ccgtgagcgc gtggatgcgg tggtggaatt tttaaatggc aattatgcgc
     2941 cggtgctaaa gtctctggaa gagaagatga atacggcgtc ggccaatctg gaatttgaaa
     3001 aggcgattga gtaccgggag cttttaaaca gtgtcagaca gatcgcacag aagcagaaga
     3061 ttacgcacac ggacggcgag gacaaggaca ttatcgcgct ggcggcggat gaccgcgacg
     3121 cggtggtgca ggtatttttt atccgcgacg gcaagctgat cggcagagat cacttttatg
     3181 tgaagatcgg gacggaggat accaaagccc agatccttac cacgttcata aagcagttct
     3241 attcgggaac gccgtttatt ccaagagaga ttatgctgcc gcaggagatc gaggagcagg
     3301 aggtgcttgc cgactggctt ggcgaaaagc ggggcagcaa ggtttacatc cgcgtaccac
     3361 agaagggcat gaaagaaaag ctggtggaac tcgcacagaa gaatgccaag atggttcttg
     3421 cgcaggatcg tgagaagatc aagcgcgagg aaggcaggac gatcggcgcg ttaaaggaga
     3481 tcgaacagct tttagatatg aagggattaa accgcgtgga ggcgtacgat atctcgaata
     3541 cgagcggctt tgagtcggtc ggctccatga tcgtgtatga gaaaggcaag ccaaagcgga
     3601 gtgattaccg gaagttcaag ctgcggacgg tgtcgggacc ggatgactat gcctccatgt
     3661 atgaggtgct gacgagaaga tttacgcatg gcatgcgtga gatggaggag atggaagaaa
     3721 aagacctgtc ggaggagtat ggcagtttca caagatttcc ggatctgatc atgatggacg
     3781 gcggccgcgg gcaggtgaac atcgcactca aagtgctgga ggagctgcat cttaatattc
     3841 cggtctgtgg aatggtcaaa gatgataatc accgcacgcg cgggctatac tatcataatg
     3901 tagagatacc catcgaccgt ggaagcgagg gatttaagct gattacgcga attcatcgtg
     3961 cttgccgtct gccag
//
LOCUS       DBMDQJ010000156         3970 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8479_length_3970_cov_1.582838, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000156 DBMDQJ010000000
VERSION     DBMDQJ010000156.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3970)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3970)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3970
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8479_length_3970_cov_1.582838"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="ACZO9R_04335"
     CDS             <1..289
                     /locus_tag="ACZO9R_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR0912330.1"
                     /translation="ILWDLTRAGGYDISSIHTDCGIEIYDADKQGTGAGGSGCGCAAV
                     TLSAHFMRQIEEGKWKRILFVPTGALLSTVSFNEGMTVPGIAHAVVIEHAG"
     gene            306..662
                     /gene="spoVAE"
                     /locus_tag="ACZO9R_04340"
     CDS             306..662
                     /gene="spoVAE"
                     /locus_tag="ACZO9R_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HIR0912331.1"
                     /translation="MDYVNAFWVGGLICALVQILMEKTKMLPGRIMVLLVCLGTLLGA
                     IQIYEPFVEFAGAGASVPLLGFGNVLWKGVKEAVDQYGFLGIFMGGFQASAVGISAAL
                     VFGYLASLIFQPKMKK"
     gene            731..1075
                     /locus_tag="ACZO9R_04345"
     CDS             731..1075
                     /locus_tag="ACZO9R_04345"
                     /inference="COORDINATES: protein motif:HMM:NF013767.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR0912332.1"
                     /translation="MDENALRAAGIDYDAALARFMGKQAIYEKYLRKLPEDAHMDAAW
                     AALEQQDEAELLEQVHAIKGLTGTLGITGLFEQSAAIVALLRAGTREELQEKMTSLKQ
                     EWDRVCETIRQA"
     gene            complement(1107..1802)
                     /locus_tag="ACZO9R_04350"
     CDS             complement(1107..1802)
                     /locus_tag="ACZO9R_04350"
                     /inference="COORDINATES: protein motif:HMM:NF019349.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0912333.1"
                     /translation="MNGTRHTGTSVAVYKDLKEQILHLELPPGSAISEIDTAAAFHVS
                     RTPVRDAFKMLEREGLLEVRPHIGTFVSLIDLNMISDILYLRETLEQAVLRDLTAGYD
                     KSQEFRIRLLLQRQCELLEQDLPAEELGRAFIINDNEFHTTLYELAGKRTVMDFLTGV
                     SSQYERFRTFLNLGDRDNLLRLYNDHIKIWSCISSRDYEGLSDVVSHHIYDGFNASTE
                     IISRHPDYFCPFH"
     gene            2356..3405
                     /gene="uxuA"
                     /locus_tag="ACZO9R_04355"
     CDS             2356..3405
                     /gene="uxuA"
                     /locus_tag="ACZO9R_04355"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008718250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HIR0912334.1"
                     /translation="MKLSFRWYGPDDKVTLENIRQIPGMHSIVTAVYDVPVGEVWSEE
                     SIAALKKQVEDAGLHFEVIESIPVHEDIKLGKPSRDRYIENYCENIRRVAKAGVKCIC
                     YNFMPVFDWTRTQLDHVLPDGSTSLVYYQEQVDAVNPLQSDSDLTLPGWDASYTREEL
                     KQVVAEYNAMSEDDLWNNLKYFLERVIPVAAECDVNMAIHEDDPCWSIFGLPRIITCE
                     KNIDRFLGLVDDPHNGITLCTGSLGCSNKNDVVKMAAKYAAMGRIHFAHLRNVAVLEN
                     GFEERAHLSSCGSLDMYAIVKALHDNGFTGYVRPDHGRMIWGETGRPGYGLYDRALGA
                     TYINGLFEAVEKASK"
     gene            3462..>3970
                     /locus_tag="ACZO9R_04360"
     CDS             3462..>3970
                     /locus_tag="ACZO9R_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008975409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR0912335.1"
                     /translation="MKLPFQIDLTGKVVVVTGAGGVICSVLAEAMAMTGAKVALLDLN
                     EDAAKKHADDITAKGYIAKGYKCNVLEKASVQAARDQIAADFGTCDILINGAGGNNPK
                     ATTDDEYFVPEDLGQMKTFFDLDADGVSFVFSLNFMGTLIPTQVFALDMVNKKGASII
                     NISSMNAYTP"
BASE COUNT         1092 a          844 c         1160 g          874 t
ORIGIN      
        1 gattctctgg gatctgacga gagccggagg gtatgacatc agcagcatcc acaccgactg
       61 cgggattgag atctacgatg cagacaaaca gggaacgggt gccggcggtt ccggctgcgg
      121 gtgtgcggca gtgacgctct cggcacattt tatgcggcag atcgaggaag gaaaatggaa
      181 aagaatcctg tttgtcccga cgggagcgct gctctcgacc gtttccttta atgagggaat
      241 gacagtgccg ggaattgcgc atgcggtcgt cattgagcat gcaggataga aaggggaaaa
      301 atgctatgga ttatgtcaat gcattctggg ttggcggact gatctgcgcg ctggtgcaga
      361 ttttaatgga aaaaacaaaa atgcttccgg ggcgcatcat ggtgcttctg gtgtgtcttg
      421 gcacactgct tggagcgata cagatctatg aaccgtttgt ggaatttgcg ggggcaggtg
      481 ccagcgtgcc gttgctcgga tttggaaatg tattgtggaa gggcgtgaaa gaggcggtgg
      541 atcaatatgg ctttcttgga attttcatgg gaggctttca ggcaagcgcg gtgggcatat
      601 cggcggcgtt ggtgtttggc tatctggcaa gcctgatttt ccagccgaaa atgaagaaat
      661 aagtgtttgc gtattggtac atctggtagt agaatcttaa tcataaaaag cgtgataggg
      721 ggagactgcc atggatgaga acgcactgcg cgcggccgga atcgattatg atgccgcatt
      781 ggcacggttt atgggaaagc aggcgattta tgaaaaatat ctgcgaaagc tgccggagga
      841 tgcacatatg gatgcggcgt gggcggcatt ggagcagcag gatgaagcgg agctgttgga
      901 gcaggtgcat gccataaaag gactgaccgg aacgctgggg attacgggac tttttgagca
      961 gagcgcggcg attgttgcgc tcttacgtgc aggaacgcgc gaagagctgc aggagaaaat
     1021 gacgtcatta aaacaggagt gggaccgcgt ctgtgagaca atccgacagg cgtaatccgc
     1081 ctgcggattg ccggctctaa aagattctag tgaaacggac agaaatagtc cggatgcctg
     1141 gagatgatct cggtgcttgc attgaagccg tcgtagatgt gatgggaaac tacatccgag
     1201 agcccctcat aatcccgcga ggagatacac gaccagattt tgatatggtc gttgtacagc
     1261 cgcagtagat tgtcgcggtc gcccagattc aaaaaggtgc ggaagcgctc gtactgggag
     1321 ctcactccgg tcagaaaatc catgacggta cgtttcccgg cgagctcgta gagcgttgtg
     1381 tggaactcgt tgtcgttaat gatgaaggcc cggccgagtt cctcggcggg cagatcctgt
     1441 tccagaagtt cgcattggcg ctgcaacagc aggcggatgc ggaattcctg ggatttgtcg
     1501 taccctgcgg tcagatctct taacaccgcc tgctctaagg tctccctcag atagagaatg
     1561 tcggagatca tgttgaggtc gatcagggag acgaatgtac cgatgtgcgg gcggacctcc
     1621 agaagcccct cgcgctcaag cattttaaaa gcatcccgca ccggggtgcg cgatacatgg
     1681 aacgcggcgg ccgtgtcgat ctcgctgatg gcagatcccg gcggaagctc cagatgaaga
     1741 atctgttctt ttaaatcttt ataaacggca acggatgtgc cggtatgtct ggttccgttc
     1801 ataacatgct cctctgtgga caatttggtg tccattctta ccattcaaaa tgtgaacata
     1861 tttctaatat aaatcccaat ggaacaaatt acaatctatt tttccggagc ccgggacatg
     1921 caccttacat atgtatacat aggatcggga aaaaacggga acgctgtttc ttggggcagg
     1981 atgaaaaaca gtgcaggaca gtagacggaa ttctggcgga tttcgataaa aagtccttcc
     2041 ggaacttaag acgcgcggaa cacggcgcaa gaagatgaaa caaggaaaaa tctgccggaa
     2101 aatatccgct tgtatacaac aagaagaaaa agcagagtcc gcggaaacat cgacgcaaat
     2161 tgtgaataat agacaaaacc gatacaaaaa attatgcaaa acggaaaaaa agaaagatgg
     2221 atgttgacgc ttgtatacaa gcatgatata gtcaagaaaa agaaacaaag aaacgtacat
     2281 ggagcaatat catagacatg tatacaagca aatatcacat gagcggaaag gaccttccgc
     2341 agaacggagg aaactatgaa attatcattt cgctggtatg gaccagatga caaggtgaca
     2401 ctggagaaca tccgtcagat cccgggaatg cactcgatcg taacggctgt gtatgacgtt
     2461 ccggtcggag aggtgtggag cgaggagagc atcgcagcac tgaaaaagca ggtggaggat
     2521 gccggacttc attttgaggt tatcgagagt attccggtac atgaggacat caagcttggc
     2581 aagccgagcc gcgaccgcta catcgagaac tactgcgaga acatcagacg tgtggcaaaa
     2641 gccggagtga agtgcatctg ctacaatttc atgccggtat ttgactggac gagaacacag
     2701 cttgaccacg tgctgccgga cggttcgacc tccctggtat attatcagga gcaggtggat
     2761 gctgtgaatc cgctgcagag tgacagcgat ctgacacttc cgggctggga tgcaagctac
     2821 accagagaag agttaaagca ggtggttgca gagtacaacg caatgtctga ggatgatctt
     2881 tggaacaatc tgaagtattt cctggagaga gtgattccgg ttgcagcgga gtgcgacgtc
     2941 aacatggcaa tccacgagga cgatccgtgc tggagcattt tcggacttcc gcgcatcatc
     3001 acctgcgaga aaaacattga tcgtttcctc ggtcttgtgg atgatccgca caacggcatc
     3061 acactgtgca ccggctccct cggctgctcg aacaagaacg atgtggtgaa gatggcggca
     3121 aaatatgcgg caatgggaag aattcatttt gcacatctgc gcaacgttgc agtgcttgag
     3181 aatggttttg aggagcgtgc acatctctcc agctgtggct ctctggatat gtatgcgatt
     3241 gtgaaagcgc tgcatgacaa tggatttacc ggatatgtga gaccggatca cggaagaatg
     3301 atctggggag agaccggaag accgggctac ggactctatg accgtgcgct tggagcaacc
     3361 tatatcaacg gattatttga ggctgtggag aaggcttcaa agtaaaaata agaatcagaa
     3421 aaataaatac cataaaaata aaaatcagga ggaagacgaa catgaagtta ccatttcaga
     3481 ttgatctgac aggcaaggtg gttgttgtga caggtgcggg cggagtcatc tgctccgtgc
     3541 ttgcggaggc aatggcaatg accggcgcaa aggtagcgct gctcgactta aatgaggatg
     3601 cggcaaaaaa gcatgcggat gacattacgg caaagggcta tattgcaaag ggatacaagt
     3661 gcaacgtgct tgagaaagcg agcgtacagg cggcgagaga ccagatcgct gcggacttcg
     3721 gaacctgcga tatcttaatc aacggtgccg gcggcaacaa cccgaaagcg acgaccgacg
     3781 acgagtattt tgtgccggag gatctcggac agatgaagac attctttgac ctggatgcgg
     3841 acggcgtctc ttttgtattc agcctgaact tcatgggtac cctgattccg acacaggtat
     3901 ttgcgcttga tatggtcaac aaaaaaggtg cgagcatcat caacatttcc tcgatgaacg
     3961 cttacacacc
//
LOCUS       DBMDQJ010000157         3944 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8531_length_3944_cov_1.954983, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000157 DBMDQJ010000000
VERSION     DBMDQJ010000157.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8531_length_3944_cov_1.954983"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(168..238)
                     /locus_tag="ACZO9R_04365"
     tRNA            complement(168..238)
                     /locus_tag="ACZO9R_04365"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Cys,seq:gca)
     gene            complement(389..856)
                     /locus_tag="ACZO9R_04370"
     CDS             complement(389..856)
                     /locus_tag="ACZO9R_04370"
                     /inference="COORDINATES: protein motif:HMM:NF042533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin-like domain-containing protein"
                     /protein_id="HIR0912336.1"
                     /translation="MRKLWITGCIVMICMGMLTACGGNKTEVVPIGDTEEADREVPYA
                     MYVNRVEGGKASVTISNYTDEEILYGTDFHLQKYIGSDWVDVSTLVPNYKFNEGVYSI
                     PAHSSVVTDVKWEWLYSTLTDGEYMLTKTVLDPKEDGTYDQATMYVTFVFGTP"
     gene            1106..2128
                     /locus_tag="ACZO9R_04375"
     CDS             1106..2128
                     /locus_tag="ACZO9R_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR0912337.1"
                     /translation="MNEEMTPDYMTGTPNTVCADARRQFSRIGLMFLVGTALIYGVQF
                     GASAIANAINPDLLSSTSYALLIVMIPMYVIAMPIMALLIKRIPAVTLPKKHMTFGQW
                     LIAFLMCYGAMYVSNYTGLILTQLISILKGSPVTNTMLEVATSSNLWVNLFIMVLCAP
                     VAEELIFRKLLIDRLAQYGEGVAVLFSGLMFGLFHGNLNQFVYAFVLGLCFGFIYVKT
                     GNIRYTIGLHMLVNFLGSVLGVAILKWLGDDFLSIASDPAGMMSYMTGNFGKLIVYFI
                     YIFLLLGVAIAGIILFIVNLKKIRFLPGMNTLPKGKRFSTTVLNVGMALYMIVWIVII
                     IVQLLQ"
     gene            complement(2222..>3944)
                     /gene="ftsH"
                     /locus_tag="ACZO9R_04380"
     CDS             complement(2222..>3944)
                     /gene="ftsH"
                     /locus_tag="ACZO9R_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR0912338.1"
                     /translation="REVDYGTFMTMTEDKKIAKVEIDESEIVFTDKDGNIYKTGPMND
                     PDLVQRLHDSGAEFASQIVEQMSPFLSALLGWIVPIAIFMIIGQFMYKRLMDKAGGGG
                     NAMMFGMGKSNAKVYVKSSEGIKFTDVAGEDEAKENLTEIVDYLHNPDKYREIGASMP
                     KGILLVGPPGTGKTMLAKAVAGEANVPFFSMSGSEFVEMFVGMGASKVRDLFRQAKEK
                     APCIVFIDEIDAIGKKRDGNMGGNDEREQTLNQLLTEMDGFEGNTGVIILAATNRPES
                     LDPALTRPGRFDRRVPVELPDLSGREAILKVHAKKIKVSDNVDFNKIARMASGASGAE
                     LANIVNEAALRAVRDGRAYATQADLEESIEVVIAGYQKKNAILTDKEKWTVAYHEIGH
                     ALVAAKQTNSAPVQKITIIPRTSGALGYTMQVEEGTHYLMNKEEIENKIATLTGGRAA
                     EEVVFDCVTTGASNDIEQATKLARAMITRYGMSEEFDMVALETVANQYLGGDASLACS
                     ADTQAQIDRKVVELVRKQHEKAAKILKDNRAKLDELAKYLYDKETITGEEFMSILNAQ
                     DGQTAEN"
BASE COUNT          904 a         1115 c          779 g         1146 t
ORIGIN      
        1 atttgaagtc tttttttcaa ggtcttcctt ataccttaag tactatatat tgtattccga
       61 aaaacattgc atccccggcg caacgtcatt tctttttctg attctgatgt aaaacaggat
      121 aacatgtaaa aaggcgtacg atttctcgta cgccattact gacccaaagg cgactgacgg
      181 atttgaaccg acgatcaggg agttgcagtc ccatgcctta ccacttggct aagtcgccct
      241 atctctctta tatgtctatt atatgcagat tcccgtcatt tatgacagat cccttctgcc
      301 ggaaagcgcc gcatcagaca cacagagatt ttaacaaaac cattgtctcc cgtcaagtca
      361 tatttttaaa ataaacaaca tctgccgcct acggcgttcc aaaaacaaat gtcacataca
      421 ttgttgcctg atcgtaagtt ccgtcttcct tcggatcaag cactgtcttc gtcagcatat
      481 attccccatc cgtcagcgtg ctgtacagcc actcccattt gacatccgtg accacgctgc
      541 tgtgcgccgg aatggaatag accccctcgt taaacttata attgggaacc agcgtcgaca
      601 cgtccaccca gtcgctcccg atatatttct gcagatgaaa atcggttccg tacaaaatct
      661 cctcatccgt ataatttgag atcgtaaccg atgcctttcc gccttccaca cgattgacat
      721 acattgcata aggtacttcc cggtctgctt cctccgtatc cccgatcggt acaacctccg
      781 ttttatttcc accgcaggct gtcagcattc ccatgcagat catcacgata cagcctgtta
      841 tccataattt tctcatgaat tttcctccga taaaatcttc ctttttatta taaccgataa
      901 atgcattgtt tgtctcgaaa gattttctta tttattttac ttgacaactt tacttgtcaa
      961 acatataatt attttgacaa gtaaagttgt caaatgcatg gctctttgtg ccgcagatgt
     1021 cacaaagccg ccacaaccgc cgacggacgg aagctctggc tcccggtttc ttcggcacac
     1081 aacgtctgcg gaattggagg attatatgaa cgaagaaatg acgccggatt acatgaccgg
     1141 cacacccaat actgtctgtg cggatgccag acgacagttt tcccgcatcg gtctgatgtt
     1201 tctggtcggg accgcgctca tctatggagt tcagttcggc gcaagcgcga tcgcaaacgc
     1261 gatcaatccg gatctgcttt ccagcacgag ttacgcgctt ttaatcgtta tgattcctat
     1321 gtatgtgatc gccatgccga tcatggcgct tctgatcaag aggattccgg ctgtgactct
     1381 tcccaaaaag cacatgacct tcggtcagtg gctgattgct tttctcatgt gctatggtgc
     1441 catgtatgtg agcaattata ccggtcttat cctgacacag ttgatcagta tcctgaaggg
     1501 aagcccggta accaatacca tgcttgaagt cgctaccagc tccaatctct gggtaaatct
     1561 gtttatcatg gtgctctgcg ccccggttgc cgaagagctg atcttccgga agcttctcat
     1621 tgaccgcctt gcgcagtatg gcgagggcgt agctgtgctt ttctccggcc tgatgttcgg
     1681 actgtttcac ggaaatctga atcagttcgt ctatgctttt gttctcggtc tgtgcttcgg
     1741 ctttatctat gtcaagaccg gcaacatccg ctacacgatc gggttacaca tgctagtgaa
     1801 cttcctcggc tctgtactcg gtgtggcgat cttgaaatgg ctgggagatg attttctctc
     1861 catcgcttcc gatcctgcag gcatgatgtc ctacatgaca ggaaactttg gaaaactgat
     1921 cgtttacttt atttacattt tccttttgct cggcgtggcg attgccggta ttattctctt
     1981 tatcgtcaat ctgaagaaaa tccgcttcct gcctggaatg aacacgcttc caaaaggaaa
     2041 acgcttttcc acaaccgtgt taaacgttgg aatggctctc tacatgatcg tctggatcgt
     2101 gattatcatt gtacagctct tgcaataatg aaaaaagctg ccgcaggaat cccgttttat
     2161 cggttctatt cctgtgacag cttttattat gcaatattta ctgctctttc ccgcagcttc
     2221 tctaattttc cgcggtctgc ccatcctgtg catttaagat gctcataaat tcttcccctg
     2281 tgatagtctc cttatcatag agatattttg caagttcatc cagctttgcg cggttgtcct
     2341 taaggatctt cgccgccttc tcatgctgct tccttaccag ttccactact tttctgtcaa
     2401 tctgcgcctg cgtgtccgct gagcatgcca gggatgcatc accgccaagg tactgattgg
     2461 ccactgtctc aagcgccacc atatcgaatt cttcactcat accataacgg gtaatcatcg
     2521 cccttgccag cttggtcgcc tgctcaatat cgttagaagc acctgttgta acacaatcaa
     2581 ataccacttc ctccgcggca cgaccgcctg tcagcgttgc gatcttgttc tcgatctctt
     2641 ccttattcat gagataatgt gtaccttcct ctacctgcat cgtataaccc aacgcaccgg
     2701 aagttctcgg aataatcgtg atcttctgta ccggagcgga attggtctgc tttgccgcta
     2761 ccagagcatg accgatctcg tgataagcca ccgtccattt ctctttgtcc gtcagaatcg
     2821 cattcttctt ctgataaccg gcaatgacaa cttcgatgct ctcttccaga tccgcctgtg
     2881 tcgcataggc tctgccgtca cgcaccgcgc gcagcgcagc ttcatttaca atattcgcca
     2941 gctccgcccc ggatgctccg gacgccattc tggcaatctt gttgaaatcc acattatccg
     3001 ataccttgat cttcttcgca tgtaccttaa gaattgcctc tctgccgctt agatccggca
     3061 gctctaccgg tacacgccgg tcaaaacgtc ccggacgcgt cagtgccggg tccagagact
     3121 ccggccggtt cgttgccgct aagataatca ctccagtgtt gccctcaaat ccatccatct
     3181 ccgtcagaag ctgattgagt gtctgctcac gctcatcatt tccacccata tttccgtcac
     3241 gctttttgcc gatggcgtca atctcatcga taaatacgat acatggcgct ttttcttttg
     3301 cctggcggaa caggtcacgc acctttgaag cgcccatgcc gacaaacatc tccacaaatt
     3361 ctgagcctga catcgagaaa aacggtacat ttgcctctcc ggcgacagcc ttcgccagca
     3421 tcgtcttacc ggttcccgga ggaccgacaa gcagaatacc ttttggcatg gatgcaccaa
     3481 tctcccggta cttgtccgga ttatgcagat aatccacaat ctccgtcaga ttctccttcg
     3541 cttcatcctc acctgccacg tccgtgaact tgattccctc cgaagacttt acgtatactt
     3601 tggcattcga cttgcccata ccgaacatca tcgcgttccc gccgccaccg gctttgtcca
     3661 tcagacgctt atacataaac tggccgatta tcatgaaaat ggcgatcggc acaatccagc
     3721 ccagcaatgc acttaaaaac ggagacatct gctctacaat ctgacttgca aactccgccc
     3781 cagaatcatg aagccgctgt accagatccg gatcattcat cggacccgtc ttgtaaatat
     3841 ttccatcctt gtccgtaaag acgatctcac tctcatcgat ctcaactttt gcaatctttt
     3901 tatcttccgt catggtcata aacgttccat agtccacttc cctg
//
LOCUS       DBMDQJ010000158         3931 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8560_length_3931_cov_1.658486, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000158 DBMDQJ010000000
VERSION     DBMDQJ010000158.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3931)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3931)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3931
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8560_length_3931_cov_1.658486"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="ACZO9R_04385"
     CDS             <1..411
                     /locus_tag="ACZO9R_04385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912339.1"
                     /translation="ALIPYYQRAGRQPPLPNEGQQPARCGSMESNIFTIIGNRMKHNR
                     TCWSVAGANNLAALLALHHTGRLRRVLRGWEAHSRAEGSGSIVTLPLSAAKANRLGGD
                     IYIPPHTLSSGEIHPAAQYYLTHFDPLSGMHSYD"
     gene            complement(1180..1950)
                     /locus_tag="ACZO9R_04390"
     CDS             complement(1180..1950)
                     /locus_tag="ACZO9R_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912340.1"
                     /translation="MAKFAKKTTLGYKPVAGGSSNSECTHVILTLDEYDGLKREISSA
                     QQAARNAEYNADKKINQIQSEAQSRITHTEKEMAKNTAEWERKLAAEQQESALQRELN
                     ANLLRITKERANAERGLKPKKEHNGYVVLTSVEKESRYKDSYGHPRTITLWETAMETP
                     YTVDFEAEQVRSLLQELFQKDEDERWPICRIGITGNYPGKYADMIQDKKWREDYEKYN
                     VMLERWLRANYRSGYWELIFQHTKPLSVVPPDMRARRG"
     gene            complement(1965..2990)
                     /locus_tag="ACZO9R_04395"
     CDS             complement(1965..2990)
                     /locus_tag="ACZO9R_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912341.1"
                     /translation="MGRRNKTYSKDLHQQAYDQLTGMLAFGESKKEAMATGTAKNKIF
                     SHATYKNYWKHIKYFLGYIKEKHPECTTLKNAKKYVNEWLQSRVDQGLSAWTVQLEAK
                     ALGKLYGISPDDEAYFDPPKRNRQDIKRSRGDRVRDQHFSKTNNDELIKFCRGTGLRR
                     RELQELRGKDLVSREQIEAELSELEKIPEAQQAPSVARRLEILRDTQLFPEAQSFIHV
                     RNGKGGRERLSPIIGKHADQIVERIADTPAEEKVWQHIHTSADIHGYRAEYATAIYKA
                     HARAIEDIPYDKVNRGTGRRYQSEVYTCRKDEAGRKLDKAAMLVCSKALGHNRISVVA
                     DNYIRGL"
     gene            complement(3744..>3931)
                     /locus_tag="ACZO9R_04400"
     CDS             complement(3744..>3931)
                     /locus_tag="ACZO9R_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912342.1"
                     /translation="SGGKLRMNGAFSDFFRSKNEEETRIRCLIWMAAMTLIGHDFKPG
                     FGLSFAAFQTEFCHIAA"
BASE COUNT          798 a         1138 c          960 g         1035 t
ORIGIN      
        1 gccctgatac cgtactacca gcgagcagga aggcagcctc cgctgcccaa cgagggccag
       61 cagcccgccc gctgcgggag tatggagagc aatatcttca ccattattgg gaaccggatg
      121 aagcacaacc gcacctgctg gagcgtggct ggagcaaaca atctggcagc cttacttgcg
      181 ctgcatcata ccggcagact ccggcgtgtt ctgcgcggct gggaggcgca cagcagagca
      241 gagggcagcg gttcgatcgt gacgctgccg ctttctgccg cgaaggccaa caggctgggt
      301 ggggacatat atatcccacc acatacattg tcctccggcg agatccatcc cgccgcccag
      361 tattatctga cacactttga tccgctgtct gggatgcact cctacgactg acaacggccg
      421 gctgcgtgcg ccggggtact tatccgctca attttttctc tttgaacaac acgtttttcc
      481 gtccgatttg actgccagcc ccccttgcca acgattttgt tacacatact aaacaaaaaa
      541 tgacagttga ttttctgaac gagttggaac ataatatcac gttggaaatg ataacatttt
      601 taaaggcggt cttccaagtg aacagaaaat cataaacaac gcaagaggac cgggtggtaa
      661 aatgggcaga aaaaaagccg acggcataag ccgacggcgc aggccaaaag aatgagacag
      721 ttcacagctg acattctggc ggccacaatt tccggcctca tcgttctggc ggttcaaaaa
      781 atgctgaact ggtaaaggcc cggggtgcgg agacacaacc ctccgcaccc caactataaa
      841 gcatgagcca ctaaactgtc aagtgggtga aaagcttaaa tatctgcttt tcgtggtcat
      901 attcgtggca gtgtttgtcc cggttcgctg tggcattcgg gcgctgcaaa ctttattaga
      961 gaagcagaaa taaagcgccg cgcgggagaa agcgggggcg ggacagtccg cgccgtaccg
     1021 cccgacgcgg cgtaagcccc aaaacccgaa ctccatagaa cccagccgcg gccggaatcc
     1081 tccggccgcg gagaggaaca aagacccccg gttcgtgcga accgggggtt ttctgcattc
     1141 agcgcaaggg cgcgcagcga gcagggaagg gggggatgct catccccgcc tcgcccgcat
     1201 atccggtgga acgacggaca ggggctttgt atgctggaag atcagctccc agtaaccgct
     1261 cctgtaattt gccctcagcc accgttccag catgacgttg tacttctcat agtcctcgcg
     1321 ccatttctta tcctgaatca tatccgcata tttgcccgga tagtttccgg tgatgccgat
     1381 ccggcagatc ggccatctct catcctcatc cttttgaaac agttcttgca gcaggctcct
     1441 gacctgctct gcctcgaaat ccacggtata gggcgtctcc atcgccgttt cccacagcgt
     1501 aatcgtccgg gggtgtccat acgaatcctt atagcgggat tccttttcca ctgacgtcag
     1561 cacaacatac ccgttatgtt cttttttcgg cttcagaccc cgctccgcat tggcccgctc
     1621 tttggtgatc cgcagcagat tggcgttcag ctctcgctgc agagcgcttt cctgctgttc
     1681 tgccgccaat ttcctttccc attctgctgt atttttcgcc atttccttct cagtatgcgt
     1741 tatcctgctc tgcgcttcgc tctgtatctg gttaatcttt ttatccgcat tgtattctgc
     1801 atttcttgcc gcctgctgtg cgctggagat ctcccgtttc agcccgtcat actcgtctaa
     1861 cgtcagaatc acatgggtac actccgaatt ggacgatccg cccgccaccg gcttataccc
     1921 taatgtggtt ttctttgcaa atttagccat gatggacgcc tcctttacag accccgaatg
     1981 tagttatctg ccaccacgct gatacggtta tgccccagcg ccttgctgca aaccagcatg
     2041 gcggccttgt ccagctttct cccggcctca tccttgcggc aggtgtacac ctcgctttgg
     2101 taacgccgcc ccgtgccgcg gttaaccttg tcatacggga tgtcctcgat ggccctggca
     2161 tgagccttgt agatcgccgt ggcatactcc gcccggtagc cgtggatgtc ggcgctggtg
     2221 tggatgtgct gccagacctt ttcctctgcc ggcgtgtccg cgatccgctc cacgatctga
     2281 tccgcatgct tgccgatgat cggactgaga cgctcccgtc cgcccttgcc gttgcgaaca
     2341 tggataaagg actgcgcctc cggaaacagc tgcgtgtccc ggaggatctc aagacgccgt
     2401 gcgacgctcg gcgcttgctg cgcctcgggg atcttctcca gctcggaaag ctctgcttcg
     2461 atctgctccc tggaaaccag atccttcccc cgcagctcct gcaattctct tcgccgtagg
     2521 ccggtcccgc ggcaaaattt tatcagttca tcattgttcg tctttgagaa gtgctgatcc
     2581 cgcacccggt ctccgcggct tcgtttaata tcctgccggt ttcttttcgg cggatcaaag
     2641 tacgcctcgt catctggaga aattccgtac agctttccga gggcctttgc ctccaattgc
     2701 accgtccatg cggagaggcc ctgatccact ctgctctgca gccactcgtt gacatacttc
     2761 tttgcattct tcaaggtggt gcactcagga tgcttctcct taatgtatcc caaaaaatat
     2821 ttgatgtgct tccaataatt tttataggtg gcgtgagaaa atattttatt cttcgccgtg
     2881 ccggtggcca ttgcctcttt tttgctctcg ccgaatgcca gcatccctgt cagctggtca
     2941 taggcttgct gatgcagatc cttggaatat gttttgtttc tgcgtcccat tccggcctcc
     3001 tgtctgttta ttttttaaat ctatttttca ctttacagag ttcttctgct cgtttgtttc
     3061 gtctgttaaa gcccacggcc gcctcctgtt cgtccctttc agagacgcaa caatcggcaa
     3121 tacacttttt ttgcctgctt gtgtgtgcag tttataggaa tcgctcccca acactttttt
     3181 cttgcttatg tgtgcagccc tatttattaa tatctacggc agttagggtt ctgtcggcct
     3241 ctttcgtaga gtgaggcgtc tctccgggag atttacagca tcccggctgc tatcacatag
     3301 ggtggatcgg gacagatacg ttcttaccga cactttcacg aagattactc atgccgggtc
     3361 cgtggcgcac ttttccattt cacaacggtt actgcggcga gcccatgcgc catataggtc
     3421 gtaccatacc tagtgcggct cctgaatcat aatctgatct gagcacagct tatcatacat
     3481 ccttatggat gtcaacccca ttttgtgcat atctgtttac ccgttcattt ataacgctcg
     3541 cggatttgat aaaaaaatgg ctgccaggac gcgattggac ggaggcggaa gatagccgcc
     3601 cgtctctttt tttgtgggga cttttggttc atccgaggca ttgatcggga cttttttcgg
     3661 ctcgactgct gcccgtcagc cccatggacg gacgctttta aaggatgaaa cgcagccgca
     3721 tttgccatca tattgccgcc cactcaggca gcaatgtggc agaattcagt ctgaaatgcg
     3781 gcgaaactta acccgaaacc cggtttgaaa tcgtggccga taagcgtcat tgccgccatc
     3841 caaatgagac agcggatgcg cgtttcttct tcattttttg agcgaaaaaa gtcggagaat
     3901 gctccgttca ttctcaattt tcctccgctt t
//
LOCUS       DBMDQJ010000159         3930 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8564_length_3930_cov_1.375033, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000159 DBMDQJ010000000
VERSION     DBMDQJ010000159.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8564_length_3930_cov_1.375033"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(379..1089)
                     /gene="trmD"
                     /locus_tag="ACZO9R_04405"
                     /pseudo
     CDS             complement(379..1089)
                     /gene="trmD"
                     /locus_tag="ACZO9R_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
     gene            complement(1100..1621)
                     /gene="rimM"
                     /locus_tag="ACZO9R_04410"
     CDS             complement(1100..1621)
                     /gene="rimM"
                     /locus_tag="ACZO9R_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080105.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR0912343.1"
                     /translation="MEDLLQVGVITTTHGVRGEVKVFPTTDDPARFKKLKNVILDTGK
                     EKLELEIAGVKFFKNMVILKFKGIDDINDVEKYRKKSLYVTRENAVKLKKDEYFIADL
                     IGMKVSTDEGEELGTLSDVMQTGANDVYVISMTDGEEVLVPAIRDCIRDVDVEQGQMC
                     VHLLPGLLDINRK"
     gene            complement(1719..1949)
                     /locus_tag="ACZO9R_04415"
     CDS             complement(1719..1949)
                     /locus_tag="ACZO9R_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HIR0912344.1"
                     /translation="MKELVEVIAKSLVDYPDQVVVTETENEKAIVLELRVAQSDMGKV
                     IGKQGRIAKAIRSVVKAAASKEEKKVIVEIMQ"
     gene            complement(1969..2214)
                     /gene="rpsP"
                     /locus_tag="ACZO9R_04420"
     CDS             complement(1969..2214)
                     /gene="rpsP"
                     /locus_tag="ACZO9R_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004079343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR0912345.1"
                     /translation="MAVKIRLRRMGQKKAPFYRIVVADSRSPRDGKCIEEIGTYDPTK
                     DPSEYHVNEELAKKWLANGAQPTDTVARIFKSAGIEK"
     gene            complement(2280..3638)
                     /gene="ffh"
                     /locus_tag="ACZO9R_04425"
     CDS             complement(2280..3638)
                     /gene="ffh"
                     /locus_tag="ACZO9R_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR0912346.1"
                     /translation="MAFDSLSEKLQNVFKNLRSKGRLTEADVKTALKEVKMALLEADV
                     NFKVVKQFVKDVEERAVGQDVMNGLNPGQMVIKIVNEEMVRLMGSETAEITLQPGKAV
                     TVIMMTGLQGAGKTTTAAKLAGKFKQKGRKVLLVAGDVYRPAAIEQLQINGQKQGVEV
                     FSMGDKNSPVNIAKAAMEHAEKNDFNIVIIDTAGRLHIDEDMMAELIQIKENVTVHQT
                     VLVVDAMTGQDAVNVAKTFDERIGIDGVILTKLDGDTRGGAALSIRAVTGKPIFYAGM
                     GEKLSDLEQFYPDRMASRILGMGDVLTLIEKAQEDFDEERAKKIEQKMRKNEFDFEMY
                     LESMSQMKKMGGLSSILGMMPGLGMGAGKMPDIDEAAAEKSLSRTEAIIYSMTPEERQ
                     NPSLLNPSRKRRIAVGAGVDIAEVNRLVKQFEQMKKMMKQMPGMMKKGRRGGGMFGKL
                     PF"
     gene            complement(3639..>3930)
                     /gene="ylxM"
                     /locus_tag="ACZO9R_04430"
     CDS             complement(3639..>3930)
                     /gene="ylxM"
                     /locus_tag="ACZO9R_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048500 - signal recognition particle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HIR0912347.1"
                     /translation="NEHQRRIYKDFVFNDLSLGEIADEEGISRQGVHDMVKRCTKTLE
                     GYEEKLHLIAKFMSAKHSVEQIHALTKQFHESHDEAVIVEIEQISNQILEEL"
BASE COUNT          794 a         1245 c          795 g         1096 t
ORIGIN      
        1 accgttttcg gccggtttgc cgtccatgcg gtacagtccg gtgacaggca gcttttcgtg
       61 ctttgtatac acgatctggt tgcaggtcat cgccgtgcga aatcgcagat atttttctgc
      121 cggttccgtc atgaactcct gatgcagcac cagaagaccg ctcccctccc cggcgtcctc
      181 ctgcaggatt tcaagcatcc tgcaaccagc tttgatgtcc tccgccgtat gaaaatacgc
      241 cccgctgact ttatcctgca gacagatatt ttccccttct gcaaaaacaa ggcgcgcctg
      301 cccgcgcgca atcagctcgg tcatatccat atgcagcagc ttgtccccgg caagcacctt
      361 tttgcaggac atcagtcgct cgtagcctgc gtagagatcc ggccgcctct cctgcgtgcg
      421 gcgctccgcc tcctgtctgc gccactcggc aatttttctc tgattgcccg acagcagcac
      481 ctttggcacc tgctttccgt gccattcctc cgggcggctg tactgtggat actctaacag
      541 atcgccctcg aaggactccg tctccccgga ctctccgttc gccagtaccc cgggcaccat
      601 ccgtgagatc gcgtctatca tcaccatcgc cggaagttcc ccgccggtca gcacataatc
      661 gccgatcgac acgtagtccg tgacggtctc ctctaataca cgctcgtcga taccctcata
      721 gtgcccgcac agaaaaataa gatcttcctc cttcgcgaac tcctttgcca tctgctggtt
      781 gaacacggct ccctgcggcg tcacatacac cgtgcgcacc ggtctgtccc gcagctcctc
      841 cggctgccgg ctccggattt cctcgcaggc tgccttccac gcatcgtaga ccggctgcgc
      901 ctgcatcagc atgccggctc ccccgccgta aggataatca tcgacttttt tatgtcgatc
      961 cgccgtataa tcccggatgt tgactgcgtt cacggacagc agtccctttt ttgcggcacg
     1021 tccaaggatg ctctccgaga gtccctgcgt caccatctcc ggaaacagcg tcagaatatg
     1081 ataattcatg ctgtgcctcc tattttcggt tgatgtcaag cagtcccgga agcagatgca
     1141 cacacatctg tccctgctct acgtccacat cacggatgca gtccctgatc gccggaacga
     1201 gcacttcctc cccgtctgtc atggagatca catagacatc gttcgcgccg gtctgcatca
     1261 catccgacag cgttcccaat tcctcaccct cgtccgtcga caccttcata ccgatcagat
     1321 ctgcaataaa gtattcgtcc tttttcagct ttaccgcgtt ctctctcgtc acataaaggc
     1381 tctttttgcg atatttttcc acatcattga tatcatcaat tcccttaaat ttgagaatca
     1441 ccatattttt aaagaacttg acgcctgcaa tctcaagttc taatttttct tttccggtat
     1501 ccaaaatcac atttttgagt tttttgaagc gcgctggatc gtccgtcgtc ggaaatacct
     1561 tgacctcccc acgcaccccg tgtgtcgtcg tgatgacacc gacctgcaat aaatcttcca
     1621 tgcatgtcct ccgtttctgt ctctgatatc cgcagtagcc gcaatatgaa caaaggagct
     1681 gccaccccat tatctggcgc gacagctcct ctgacctgtt actgcatgat ctctacaata
     1741 actttcttct cttcctttga ggcagcagcc ttcacaacag aacggattgc cttggcaatg
     1801 cgtccctgtt tgccgattac cttgcccatg tcggactgcg caacgcgcag ttcaagcacg
     1861 atggctttct cattctctgt ctctgttacg acgacctgat ccggataatc gacgagtgat
     1921 ttcgcaatta cttctactaa ttctttcatt aggtccacct cgcgaatcct atttctcgat
     1981 gcctgcgctc ttgaaaatac gagcaactgt atcggtcggc tgagctccgt tagctaacca
     2041 cttctttgct aactcctcat ttacatgata ctcgcttgga tctttcgttg gatcgtatgt
     2101 tccgatctcc tcgatgcact tgccatctct cggggatctg gaatctgcta ctacgattct
     2161 atagaaagga gctttcttct gtcccattct tcttaatctg atctttactg ccattacttt
     2221 cacctcctta aatttgctta actgatctat ttagaatgcg ttatacgcaa tttctaacat
     2281 taaaacggaa gcttgccgaa cattccacca ccgcgtctgc ccttcttcat catgccaggc
     2341 atctgcttca tcatcttctt catctgttca aactgcttca ccagacggtt gacctccgca
     2401 atatcgacac cggctcccac cgcaatacgg cgcttcctgc tcggattcaa aagggaagga
     2461 ttctgtctct cctccggcgt catggaatag atgatcgcct ccgtgcgcga taagctcttc
     2521 tcggctgctg cctcgtcaat atccggcatc ttgcctgcgc ccatcccgag tcccggcatc
     2581 ataccgagga tactggacag accgcccatc ttcttcatct gactcatcga ctccagatac
     2641 atctcaaagt caaattcgtt cttgcgcatc ttctgctcaa tcttcttcgc ccgctcctcg
     2701 tcaaagtcct cctgggcttt ttcgatgagg gtcagcacat cgcccatacc gagaatacgc
     2761 gatgccatgc gatccgggta aaactgctcc agatccgaaa gcttctctcc catacccgca
     2821 taaaaaatcg gcttaccggt cactgcccgg atggaaagcg ccgcaccgcc tctggtatca
     2881 ccgtcgagct ttgtcagaat gactccgtcg ataccgatgc gctcatcaaa ggtcttcgcc
     2941 acattcaccg catcctgtcc tgtcatggcg tccaccacaa ggacggtctg atgcactgtg
     3001 acattttctt ttatctgaat caactctgcc atcatatctt catcgatgtg aagacgtccg
     3061 gcagtatcga tgataacgat gttaaaatcg ttcttctcgg catgttccat cgccgctttt
     3121 gcaatgttca ccgggctgtt cttatcgccc atggaaaata cttccacgcc ctgcttctgt
     3181 ccgttgatct gcagctgttc gatcgctgcc ggccggtaga catcacctgc cacgagaagt
     3241 accttcctgc ctttctgctt gaactttccg gcaagctttg ctgctgtggt cgtcttacct
     3301 gctccctgaa gaccggtcat catgatgacg gtaactgcct tgccgggctg cagtgtaatc
     3361 tcggctgtct ccgagcccat cagcctgacc atctcttcgt ttacaatctt gatgaccatc
     3421 tgccccgggt tcagtccgtt catcacgtcc tgtccgacgg cacgctcctc tacgtccttg
     3481 acgaactgct tgacgacctt gaagttgaca tccgcctcta agagcgccat cttgacttcc
     3541 ttaagcgctg tcttgacatc cgcttccgtc agccgtccct tgctgcgcag gttcttgaat
     3601 acattttgaa gtttttcaga taagctgtca aatgccatct ataactcctc taatatctga
     3661 ttggaaatct gttcaatctc cacgatcact gcctcgtcat ggctctcatg aaactgcttc
     3721 gtcaatgcat gaatctgttc cacactgtgc ttcgccgaca tgaactttgc gatcagatga
     3781 agcttctcct catacccctc aagggtcttc gtacaccgct tcaccatatc gtgaacgccc
     3841 tgtctgctga taccttcctc gtccgcgatc tctccgaggg acagatcatt gaacacgaaa
     3901 tccttataaa ttcttctctg atgttcgttc
//
LOCUS       DBMDQJ010000160         3921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8586_length_3921_cov_1.668325, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000160 DBMDQJ010000000
VERSION     DBMDQJ010000160.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8586_length_3921_cov_1.668325"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            195..>465
                     /locus_tag="ACZO9R_04435"
     CDS             195..>465
                     /locus_tag="ACZO9R_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0912348.1"
                     /translation="MTNVTKSYATGAPALNGIDLHIKPGEFVFIVGDSGSGKSTMIKL
                     LLRELVPTSGEISVMGYDLLKIRHRKIPKFRRNLGIVFQDFRLLKD"
     assembly_gap    466..475
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <476..768
                     /locus_tag="ACZO9R_04440"
     CDS             <476..768
                     /locus_tag="ACZO9R_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR0912349.1"
                     /translation="AKPRQLSGGEQQRVAIARALVNRPSILLADEPTGNLDPKNSWEI
                     MKLLEQINENGTTVLVVTHNREIVNAMQKRVVTMKKGIIVSDEEKGVYIDED"
     gene            758..1654
                     /gene="ftsX"
                     /locus_tag="ACZO9R_04445"
     CDS             758..1654
                     /gene="ftsX"
                     /locus_tag="ACZO9R_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HIR0912350.1"
                     /translation="MKISTLFYTIKQGFINIFRNKWFSLASIATIGACLFLFGLFYSV
                     LMNFQNIVKTAQEGVCVTVLFDEGISQERIEEIGALIDKRPEVSKKEFISADDAWNSF
                     KEEYLQGYEDGFTENPLADSASYEIYMNDISMQPTLVSYLEGLEGVREVNRSEVTASA
                     LTGVNALIAYVSIGIIAILLAVSVFLISNTVTIGISVRKEEITIMKYIGATDYFVRAP
                     FVIEGMLIGAIGALIPLGIIYLLYNKVIEYIEQRFSILTTILAFLPVDAIFKTLLPLS
                     LLIGIGIGFLGSITTVRKHLRV"
     gene            1671..2273
                     /locus_tag="ACZO9R_04450"
     CDS             1671..2273
                     /locus_tag="ACZO9R_04450"
                     /inference="COORDINATES: protein motif:HMM:NF048395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR0912351.1"
                     /translation="MKRTIKWICLFLCVVLVFGMFAKPALATYASELTNSVIKEKEQE
                     IDKAKQEKKQLQNSLTDVKKLKEQLEKSKQDLTVYVQELDADLTAIQEKINELNDLIT
                     EKEKEIEDKTAELEEAQRVQQEQYEAMKTRIKFMYERGDTIYMELIFSSNSFGDVLNK
                     ADYIQMLSEYDRKLLDEYVAHANYVALCKESLEEEKQLLK"
     gene            2732..3274
                     /gene="rbr"
                     /locus_tag="ACZO9R_04455"
     CDS             2732..3274
                     /gene="rbr"
                     /locus_tag="ACZO9R_04455"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005604292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR0912352.1"
                     /translation="MKETKYAGTQTEKNLMTAFAGESEARNKYTYFASKAKKEGYEQI
                     AALFLKTAENEKEHAKLWFKELDGIGDTAENLRSAAEGENYEWTDMYDGFAKTADEEG
                     FHELAQRFRLVAAIEKHHEERYRALLRNVEMAQVFAKSEVKVWECRNCGHIVVGEKAP
                     EVCPTCNHPQSYFEIHAENY"
     gene            3289..3663
                     /locus_tag="ACZO9R_04460"
     CDS             3289..3663
                     /locus_tag="ACZO9R_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HIR0912353.1"
                     /translation="MEQKFYICKHCGNIIAKVKDAGVPVICCGEPMSEIIPGTTDAAV
                     EKHVPVWTVENGIVHVKVGSMEHPMLPEHYIEWVSLHTKQGNQRKELHPGEKPEVCFA
                     LCEGDKVEAVYAYCNLHSLWKA"
BASE COUNT         1141 a          810 c         1044 g          916 t
ORIGIN      
        1 gaaaacaacg ggactggatc ttcggatctt tgaagatgcc attaccttcc ggattgcact
       61 gatggttgtg aaatacatga actttatgga aaattttgaa tattaagata caggcggtgg
      121 atatagtggc taagagtgaa ccaaggaata gaagaaaaaa gaaacaggga agcggaaatg
      181 aagatattat ttccatgacg aatgtgacca agtcctatgc aacaggtgcg cctgccctga
      241 atggaattga tctgcatatt aagccgggtg aattcgtatt tattgtagga gacagcggat
      301 ccgggaagtc taccatgatt aagcttcttt taagagagct tgtcccgaca tccggtgaaa
      361 tcagtgtcat gggctatgat cttttgaaaa tccgtcatcg taagattcct aaattccgca
      421 ggaatttagg aattgtgttc caggatttcc gccttctgaa ggatcnnnnn nnnnnaggcg
      481 aagccccgtc agttatcagg tggtgagcag cagcgtgtag cgattgcaag agcgctggtg
      541 aaccgtccga gcattttgct cgctgatgag cctaccggta atctggatcc gaagaattca
      601 tgggaaatta tgaagctgct ggaacagatt aatgagaacg gtacgacggt acttgttgtt
      661 acccacaaca gagaaattgt aaacgcaatg cagaaaagag tcgttaccat gaagaagggt
      721 attattgtga gcgacgaaga gaagggtgtt tatatcgatg aagattagta cattgtttta
      781 tacgattaag cagggattta ttaatatatt ccgtaataaa tggttttcac tggcctcgat
      841 cgccacgatc ggagcatgtc tgttcctgtt tggtctgttt tattcggtac tgatgaactt
      901 tcagaacatt gtgaagactg cgcaggaagg tgtctgcgtg acggttctgt ttgacgaggg
      961 aatcagtcag gaacggattg aagagatcgg agcattgatt gataagcgtc cggaggtttc
     1021 caagaaggag ttcatttctg cagatgatgc atggaacagc tttaaggaag agtatcttca
     1081 gggctatgag gatggattta ccgagaaccc gcttgccgat tctgccagct atgaaatata
     1141 catgaatgat atttccatgc agccgacatt ggtttcctat ctggaaggcc tcgagggtgt
     1201 gcgtgaagtg aaccgttctg aagtaacggc atcggcgctg accggtgtga atgcgttgat
     1261 tgcctatgtt tccattggta ttattgcaat tctgcttgcg gtatccgtgt tcctgatcag
     1321 taatacagta accattggta tttctgttcg taaggaagag attaccatta tgaaatatat
     1381 tggggcgaca gattattttg tccgtgcacc gttcgttata gaaggtatgc tgatcggtgc
     1441 gattggagct ctgattccat tgggaattat ttatctttta tataataagg taattgaata
     1501 tattgaacag agatttagta ttttgacaac gattctggca ttcttaccgg tagatgccat
     1561 ctttaagaca ctgcttccgt tatcccttct cattggtatc ggaattggat tcctgggaag
     1621 tatcacgacg gtaagaaagc atctgcgggt ataatagata aggacagagt atgaaacgta
     1681 caataaaatg gatctgcctg ttcctttgtg tagtgctggt ttttggaatg tttgcgaaac
     1741 cggcacttgc cacatatgcc agcgaactga ccaacagtgt cattaaggaa aaggaacagg
     1801 aaattgataa ggcaaaacaa gagaaaaaac agttacagaa cagtctgaca gacgtgaaga
     1861 agctgaagga acagctggag aagtccaagc aggatctgac agtttacgta caggagctgg
     1921 atgcggatct gacggcaatt caggagaaga ttaacgaact gaatgacctt atcaccgaaa
     1981 aagaaaaaga aattgaagat aagacggcag agctggaaga agcacagcgt gtccagcagg
     2041 agcagtatga ggcaatgaaa acccgtatca aatttatgta tgagagggga gacacgattt
     2101 atatggagct gattttcagt tccaattctt tcggagatgt gttgaataaa gctgattata
     2161 tccagatgct ttccgaatat gaccgtaagc ttctggatga atatgtcgca catgccaatt
     2221 atgttgcctt atgcaaggag agcctggaag aggagaaaca gcttctcaaa taacagcgtc
     2281 tcaggcagcg gcgtaaggga agcgtgactc ccttgacgga acgatgtgac gatgcataca
     2341 ggaatggaga ttgcagtgat ttttgaaata aatattgctt tttctggggc caaaacggta
     2401 taatcaatat gtaacaaaaa caacggaaaa gcgtctatgc taaggctgcc gcgagccgtc
     2461 gggttgtgac ataccaaagc ttgatacatt ctgccccaac tggaacagat actggagagg
     2521 atacaatagg cgagcggatc ggcacggcac atcagcccgg cgcggatacg caagaatttc
     2581 tcggaggacg gactggtgga actgaagcgc ggcgcgatca cgctgcggga taagaaccag
     2641 cccaaactgc ttgaaataat gtgattttgt tacagaacgg aatcataatt ccgatataat
     2701 gaaagtagga aaaccaaaag gggaattgac catgaaagaa acgaaatacg caggaaccca
     2761 gaccgaaaaa aacctgatga ctgcctttgc cggcgagtcc gaggcccgca acaagtacac
     2821 ctacttcgcc tccaaggcca agaaggaggg ctacgagcag attgcggcgc tgttcctgaa
     2881 aaccgccgaa aacgaaaagg aacacgccaa gctctggttc aaggagctgg atggtatcgg
     2941 cgacaccgcc gaaaacctcc gctccgccgc tgagggcgaa aactacgaat ggacggatat
     3001 gtacgacggc ttcgccaaga ccgccgacga ggaaggcttc catgagctgg cccagcggtt
     3061 ccgtctggtg gctgccatcg agaagcacca cgaggagcgc taccgtgccc tgctgcgcaa
     3121 tgtggagatg gcgcaggtct tcgccaagag cgaggtcaag gtgtgggagt gccgcaactg
     3181 cggtcacatc gtcgtgggcg agaaagcgcc ggaggtctgt ccgacctgca accatcccca
     3241 gagctacttt gaaattcatg cggagaacta ttaaaagagg aaatcatcat ggagcagaag
     3301 ttttacattt gcaagcattg cggcaacatc atcgccaagg tcaaggacgc aggcgtcccc
     3361 gtcatctgct gcggtgaacc catgagcgag atcatccccg gcaccaccga cgccgccgtg
     3421 gagaagcacg tccctgtctg gactgtggaa aacggcatcg tccatgtcaa ggtcggctcc
     3481 atggagcatc ccatgctgcc ggagcactac atcgagtggg tgtccctcca caccaagcag
     3541 ggtaaccagc gtaaggaact gcatcccggc gaaaagccgg aggtctgctt tgcgctctgc
     3601 gagggcgaca aggtcgaggc ggtttacgcc tactgcaacc tccacagcct gtggaaagca
     3661 taattcatcc tcttaaaatc cggtgctgaa aaaagcaccg gattttttat caatgtaacc
     3721 tcgtcacata aaaaagattt gtcctgcggt atgataaagc cacaattaag aaagaggtgg
     3781 caaacatgaa aatcaaagct caatcccgat cagaaggtcg tgaataccat ccgcgagggc
     3841 ctaaaacgta ccggcgggta ctgtccctgc cggctggagc acacggagga aaacaggtgt
     3901 atgtgtcagg agttcaagga t
//
LOCUS       DBMDQJ010000161         3913 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8606_length_3913_cov_1.518101, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000161 DBMDQJ010000000
VERSION     DBMDQJ010000161.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8606_length_3913_cov_1.518101"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1659
                     /locus_tag="ACZO9R_04465"
     CDS             <1..1659
                     /locus_tag="ACZO9R_04465"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR0912354.1"
                     /translation="DPYLSSWSPYHGAVYAVLESLSRIVSAGGDYSKVRFTFQEYFRR
                     MSEEPKRWSQPFAALLGAYNAQIGFGLPSIGGKDSMSGSFNEIDVPPTLVSFAVDVAK
                     EGDIITPELKAVGNQLVLFSIERDSYDLPVYAQVMKLYAVLHELIGKGAVVSAYALDG
                     KGLAAAVSKMAFGNKLGVTIEKEVSEETLFAPGFGNIVAEVPVEKVGIVKETLKAAGL
                     SGNEELVGWVNEEQSFCYGGMKLALDEAIAAWSGTLEKVFPTRVTDNKDELDTGIYQA
                     DSIYVCKHKVAKPTVFIPVFPGTNCEYDSARAFERAGADTIVKVFKNLNAEDIRDSVE
                     EFTKAIDQAQIIMFPGGFSAGDEPEGSAKFFATAFRNAKMTEAVMKLLNERDGLALGI
                     CNGFQALIKLGLVPYGEIRPQTADSPTLTYNTIGRHISKMVYTKVVTNKSPWLAQAKT
                     GEVYCNPASHGEGRFVAPKEWLEKLFANGQVATQYVNEAGVPTMDEEWNVNGSYMAIE
                     GITSPDGRVLGKMAHSERRDRSVAMNIYGEQDLRLFESGVEYFK"
     gene            1826..2704
                     /locus_tag="ACZO9R_04470"
     CDS             1826..2704
                     /locus_tag="ACZO9R_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002603934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR0912355.1"
                     /translation="MNILVLGGTGFFGKHLVWELLHRGHEVTIATRGRTPDDFGDRVR
                     RLIVDRTDVRQMEQVFAHKYYDVFYDDLAYCAGDVRTVLEAVPCRRYVMVSSASVYDL
                     HFQTVETDYEPEHDRLVWYTDYSGSYDVLKKSAECALVQQYPMKNAAFVRFPYVIGRD
                     DYTDRLYFYVEHVVRQKPMYIDNMDAQMSFISVDDAGRLLAHLGGDEIQGAVNGASRG
                     TISPREILTYVYRRTGKEAFLDETGDPAPYNGTPGYSINTERAGRTGFVFSNLKDWIY
                     ELLDFYIERAAEEMRK"
     gene            complement(2767..3573)
                     /locus_tag="ACZO9R_04475"
     CDS             complement(2767..3573)
                     /locus_tag="ACZO9R_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HIR0912356.1"
                     /translation="MLWDTHMHSQYSGDSDAPQEDMIRAAIRAKLPGICFTDHLDIDY
                     PDNPEAFLLDLPNYTSSVFAMQEQYRDQISVRYGIELGLQPHLAALHADILSQYDFDF
                     VIGSSHVVHGYDPYFPPFWKTHTEEEGYREYFESILENIRAFDDFDVYGHIDYVVRYG
                     PTRNENYTYARYSDVIDEILRLLIEKGKGIEINTGGFKYGLGHPNPCEEILARYRELG
                     GEIITVGADAHAPEHVGFAFEKVPQILKDAGFIYYTVFRKRKPEFIKIED"
     gene            complement(3598..>3913)
                     /locus_tag="ACZO9R_04480"
     CDS             complement(3598..>3913)
                     /locus_tag="ACZO9R_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912357.1"
                     /translation="GTLQIMREEKLYGALFFPVAFVGTVLALFYVSLQKITYDREKIV
                     ISRFPRRTQTLLRRDIRQLECTSGSQGLRTLTLHTDNRRYVIREKQFRVGFPEFFNEL
                     IH"
BASE COUNT          949 a          891 c         1201 g          872 t
ORIGIN      
        1 gatccgtatc tgtcttcctg gagcccgtat cacggtgcgg tgtatgcagt gcttgagtct
       61 ctgtcgagaa tcgtgtctgc aggcggcgat tacagcaagg tgcgctttac cttccaggag
      121 tatttccgca ggatgagcga ggagccgaag cgctggagtc agccgtttgc tgctctgctt
      181 ggcgcgtaca atgcacagat cggcttcgga ctgccgtcca tcggaggaaa ggattccatg
      241 tccggaagct tcaacgagat cgacgtaccg ccgacactgg tatcctttgc cgtggatgtg
      301 gcaaaagagg gcgacatcat cacgccggag cttaaggcag tgggaaatca gctggtactt
      361 ttctccatcg agagggattc ttatgatctt ccggtctatg cgcaggtaat gaagctctat
      421 gcggtgctgc atgagctgat cggaaagggt gcggtcgtat ccgcctatgc gctggatgga
      481 aaaggacttg cggcggcagt cagcaagatg gcatttggca ataagcttgg tgtgacgatt
      541 gagaaggaag tgtcggaaga gacactgttt gcaccgggat ttggtaatat cgtggcggag
      601 gttccagtgg agaaggttgg tattgtcaag gagacattaa aggcagcggg actttccgga
      661 aatgaggagc ttgtcggctg ggtaaatgag gaacagagct tctgctacgg cggcatgaag
      721 cttgcactcg acgaggcgat cgcagcatgg agcggcacgc tggagaaagt attcccgacc
      781 cgcgtgacag ataataagga cgaacttgac accggcatct atcaggcaga cagtatctat
      841 gtctgcaaac acaaggtggc aaaaccgacc gtgtttatac cggtatttcc gggaacgaac
      901 tgcgagtacg acagtgcgag agcatttgag cgcgcaggtg cggacacgat cgtaaaggta
      961 ttcaagaacc tgaatgcaga ggacatccgc gattcggttg aggagttcac aaaggcgatc
     1021 gatcaggcac agattatcat gttcccgggc ggattctccg cgggagatga gccggagggc
     1081 tccgcgaagt tctttgcgac cgcgttccgc aatgcgaaga tgacagaggc ggtcatgaag
     1141 cttttgaacg agcgcgacgg actggcgctc ggtatctgca acggtttcca ggcactgatt
     1201 aagctggggc tggtaccgta cggagagatc cgtccgcaga ccgcagattc accgacatta
     1261 acctacaaca cgatcggccg tcatatcagc aagatggtat acacgaaggt tgtgacgaac
     1321 aagtccccgt ggctggcaca ggcaaagacc ggcgaggtat attgcaatcc ggcttcccac
     1381 ggcgagggac gttttgtcgc tccgaaggaa tggctggaga agctgtttgc aaacggtcag
     1441 gtggcaacac agtatgtcaa cgaggcgggt gttccgacca tggatgagga gtggaatgtc
     1501 aacggctcct acatggcaat cgagggaatc acaagcccgg acggaagagt gctcggcaag
     1561 atggcacatt cggagcgtcg tgaccggagt gtggcgatga atatttacgg tgagcaggat
     1621 ttgagactgt ttgagtccgg cgtggaatat tttaagtaaa agaaaaaata acgggaagta
     1681 aaagagaccc tgcagcgagg agaaaaacct ttctgcgggg tcttttgccg ttcggttctt
     1741 gggggattgt tgataaaaca acgcaaaatg agtataatag tgcgaaaggg aacggaaaac
     1801 gggaagaaga aagatggaga aacgtatgaa tattttagtg ctgggcggaa cgggattttt
     1861 cggaaaacat ctggtgtggg aacttttgca ccggggacat gaggtgacga tcgcaacacg
     1921 cggccggacg ccggatgatt ttggagacag agtcagacgt ctgatcgtcg accggacgga
     1981 tgtccggcag atggagcagg tgtttgccca taaatattat gacgttttct acgacgatct
     2041 ggcttactgt gcgggggatg tgcgcaccgt gctggaggcg gtgccctgca gaaggtatgt
     2101 gatggtgtcc tcggcatccg tgtatgatct gcattttcag accgtggaga cggactatga
     2161 gccggaacat gacagactgg tctggtatac ggattacagc ggatcgtatg acgtattgaa
     2221 gaaaagtgcg gaatgtgcgc tcgtgcagca gtatccgatg aaaaatgcgg cttttgtgag
     2281 attcccctac gtcatcggca gggatgacta cacggaccgg ctgtactttt atgtggaaca
     2341 tgtggtgcgg cagaaaccga tgtatattga taatatggac gcacagatga gctttatcag
     2401 cgtggacgat gcggggcgcc ttctggcgca tctcggcggg gatgagatcc agggtgccgt
     2461 caacggagcg agccgcggga ccatctcgcc gcgggagatc ctgacttacg tgtacagacg
     2521 caccggaaaa gaggcgtttc ttgatgagac cggcgatccg gcgccatata atgggacgcc
     2581 cggctacagc atcaatacag agcgcgccgg gcgtacggga tttgtgtttt ccaatctgaa
     2641 agactggatc tatgaactgc ttgattttta catagagcgc gccgcagaag agatgcgtaa
     2701 ataaaaacag gacatcaacg tataaaatgt cagtgagaat gaggactgga gcgccggatg
     2761 cggcggtcag tcctcaattt ttatgaattc cggcttgcgt ttccggaaga ccgtgtagta
     2821 aataaaaccg gcgtccttta aaatctgagg cactttctca aaggcaaagc cgacatgttc
     2881 cggggcatgg gcgtccgcgc cgaccgtgat gatctcaccg ccaagctcac ggtagcgcgc
     2941 gaggatctcc tcgcaggggt tcggatgtcc cagtccgtat ttgaagccgc cggtattgat
     3001 ctcgatgcct ttcccttttt caatcagaag tcttagaatc tcgtcgatca cgtcgctgta
     3061 tctggcgtag gtataattct cgttgcgcgt cggaccgtag cgcacgacat agtcgatgtg
     3121 accgtagacg tcaaagtcat caaatgcccg gatgttttcc aggatggact caaagtattc
     3181 gcggtatcct tcctcctcgg tgtgcgtctt ccagaatggc ggaaaatacg gatcatagcc
     3241 gtgtaccaca tgggaggaac cgatgacaaa gtcgaagtca tactgcgaga gaatatcggc
     3301 atgcagcgct gccagatgcg gctggagccc cagctcgatg ccgtagcgca cgctgatctg
     3361 atcccggtac tgttcctgca tggcgaaaac ggatgacgtg tagttcggca ggtctaacag
     3421 gaacgcttcc gggttgtccg gatagtcgat gtccagatga tccgtaaaac agatgccggg
     3481 gagctttgcc ctgattgcag cgcggatcat gtcctcctgc ggggcgtcgc tgtcaccgga
     3541 atattggctg tgcatatgtg tatcccataa catagagatc ttcctttctg tgtgagttta
     3601 atggatcagt tcgttgaaaa attccgggaa ccctacgcgg aactgtttct cgcggatcac
     3661 atatctgcgg ttatctgtgt gcagggtaag cgtccggagc ccctgactgc cggaggtaca
     3721 ttcaagctgg cggatatccc ggcgcagcag ggtttgtgtc cggcggggga aacgggaaat
     3781 aacaattttt tcgcggtcgt aggtgatttt ctgcagactg acataaaaca gtgccagaac
     3841 ggtgccgaca aacgcgaccg gaaagaaaag ggcgccgtag agcttttctt cgcgcatgat
     3901 ctgcagcgtt ccc
//
LOCUS       DBMDQJ010000162         3889 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8666_length_3889_cov_1.484161, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000162 DBMDQJ010000000
VERSION     DBMDQJ010000162.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8666_length_3889_cov_1.484161"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="ACZO9R_04485"
     CDS             <1..128
                     /locus_tag="ACZO9R_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA ligase subunit PheS family protein"
                     /protein_id="HIR0912358.1"
                     /translation="NEYSGFAFGIGIERIAMLKYGINNIKLLFESDLRVLKQIDD"
     gene            154..462
                     /locus_tag="ACZO9R_04490"
     CDS             154..462
                     /locus_tag="ACZO9R_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013734.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912359.1"
                     /translation="MLAPLSWLKDYVDIDVTPKELEEKLFSCGFEVEELIEVGKDISN
                     VVVGLVESCEPIPDTHLSLCQVNAGEHGTFQICCGADNVRAGGKYPLALVGATVYATA
                     "
     gene            469..2571
                     /gene="pheT"
                     /locus_tag="ACZO9R_04495"
     CDS             469..2571
                     /gene="pheT"
                     /locus_tag="ACZO9R_04495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR0912360.1"
                     /translation="MTIEGVMTIKKGKLRGYESYGMLCSGTELGLNEDLYPGAGYNGL
                     LVLPEDAKVGADVKPILGMDDWIFDIAITANRPDCQSIYGIAREVAAVLGKECREPAL
                     DYTETAVKKDFKVEVAAKDLCPRYIAHYVHDVTIGESPAWMRRRLALVGINSISNMVD
                     ITNYVLKELGQPMHAFDYAYLEGDQINVRRANEGERIVTLDEKEFTLNSSNLVICDGV
                     KPVALAGIMGGLNSEIKDTTTEVMFESAKFARDNIRKSSRALGQSSDSSAMYAKGVYE
                     YTTVMAMKRALHLVEELGCGKVSSTQLEVSTGNSIEPKEMKVSVKRVNGVLGIEVPEE
                     DMLRILSALQFAPSIDGDELTLQIPAYREDMDSYPDVAEEVIRMYGYEHVIPTFMPTA
                     KVTLGGLNLKQKTELKIKRALCAAGAYEGVHYSFFSPSDLDLLRLPEDAPERKAIKLI
                     NPINVDLSLMRTTLASEMLYAISRNQKKGILEGRIFELGNVFIPKALPLTEYPDERET
                     LCAGVFGEKESFYTLKGLAETVADALNVSFTYEAAERTFLHPYQTAEIFCEGERVGYL
                     GKLSYEIQDELDMRVPAYVMEIDLAALKKWYGKEQIFTPLPKFAEEKRDFAFVVDKNI
                     TCAQIENGIREACKYVTDVNLFDVYEGIQLGPDKKSMAFSVVFTPQEEELTGEMVEGF
                     VKKILKHLEATLDIRLRA"
     gene            2583..3614
                     /gene="queA"
                     /locus_tag="ACZO9R_04500"
     CDS             2583..3614
                     /gene="queA"
                     /locus_tag="ACZO9R_04500"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HIR0912361.1"
                     /translation="MDVKDFYYDLPQELIAQDPLEDRSSSRLMVLDKATGEVEHRHFK
                     DIVDYLRPGDCLVINNTKVIPARLYGVKEGTEAKIEILLLKRKENDVWETLVKPGKKC
                     KIGTRINFGEGLLTGEVIDIVEEGNRLIQFHYEGIFEEILDQLGQMPLPPYITHQLKD
                     KNRYQTVYAKYDGSAAAPTAGLHFTPELLEQVRAMGVEIAEVTLHVGLGTFRPVKESD
                     VLKHHMHSEFYRIEQSEADKINRAKQAGHRIIAVGTTSTRTLESAADEHGMLHETSGW
                     TEIFIYPGYQFKVIDALITNFHLPESTLVMLVSALAGREHVLHAYEIAVQERYRFFSF
                     GDAMFITAK"
     gene            3629..>3889
                     /locus_tag="ACZO9R_04505"
     CDS             3629..>3889
                     /locus_tag="ACZO9R_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HIR0912362.1"
                     /translation="MLEKRKHKRLKMEHALELARLNADGTADDGVVNADILNISRGGI
                     GFLSRKSLEVGSYYDTRISLFSREMIDAVLEIVHVEEQEKGYY"
BASE COUNT         1025 a          849 c         1145 g          870 t
ORIGIN      
        1 cgaatgagta cagcggtttt gcgttcggta tcggtattga gcgaattgcc atgttaaaat
       61 acggcattaa caatatcaag ctgctgtttg agtcagatct tcgcgtatta aagcagattg
      121 acgattaaag acgaatagaa aaggagagtt atcatgttag caccattaag ctggttaaaa
      181 gattatgtag atattgatgt taccccgaag gaactggagg aaaagttgtt ttcctgcggc
      241 tttgaggtgg aggagctgat tgaagtcgga aaagacatca gcaatgttgt ggtcggactg
      301 gtggaatcct gcgagccgat cccggatacc catttaagtc tctgccaggt gaatgccgga
      361 gagcacggca ccttccagat ctgctgcggg gcagacaatg tccgtgcagg aggaaaatat
      421 ccgctggcgc ttgtgggcgc aacagtctat gcgaccgcat aggatcatgt gacgatcgag
      481 ggcgtcatga cgatcaagaa gggaaagctt cgcggctatg aatcctacgg tatgctgtgt
      541 tccggaacag aactcggatt gaacgaagat ttatatccgg gggcaggata caacggtctt
      601 ttagttcttc cggaagatgc gaaagtcgga gcagacgtaa agccgatcct tggaatggat
      661 gactggattt ttgatattgc gatcaccgca aaccgcccgg actgtcagag catttacgga
      721 attgccaggg aagtggctgc agtgctcgga aaagagtgca gggagccggc gttggattac
      781 acggagacgg cggttaaaaa agactttaag gtggaagtag ccgcaaaaga tctctgcccg
      841 cgctatatcg cgcactatgt ccatgacgtg acgattgggg agtccccggc gtggatgcgc
      901 agaagactgg cgttagtcgg aatcaactcg atttccaaca tggtcgacat cacaaactac
      961 gtgttaaaag agctcggaca gccgatgcat gcatttgact atgcttatct ggagggcgat
     1021 cagatcaatg tcagacgtgc aaacgagggc gagcgcattg tgacgctgga tgaaaaagaa
     1081 tttacgctca attcttccaa tcttgtcatc tgcgacggcg taaagccggt tgcactggct
     1141 ggtatcatgg gcggtctgaa ctccgagatc aaggacacga caacggaagt catgtttgag
     1201 tccgcaaagt tcgcgcgcga caatatccgg aagagttcaa gagcgctcgg acagtcctcc
     1261 gattccagtg cgatgtacgc aaagggcgtc tatgagtaca cgacggtcat ggcgatgaag
     1321 cgtgccctgc atcttgtgga agaactggga tgcggaaaag tgtcgtccac acagcttgaa
     1381 gtctccaccg gcaattctat cgagccgaaa gagatgaagg tcagcgtaaa gcgtgtcaac
     1441 ggtgtgctcg gcattgaggt tccggaggag gacatgctgc gcattttatc ggcgcttcag
     1501 ttcgcaccgt ccatcgacgg ggatgaactt accctgcaga ttccggcata ccgcgaggat
     1561 atggattcct acccggatgt ggcggaggaa gtgatccgca tgtacggtta tgagcatgtg
     1621 attccgacct ttatgccgac ggcaaaagtg accctgggag gtcttaactt aaagcagaag
     1681 acggagctta agatcaagcg ggcactctgc gcagcgggcg cttacgaggg agtccattac
     1741 tcattcttct ccccgtccga tctggatctg ttaagactgc cggaagacgc gccggagaga
     1801 aaggcgatca agctgatcaa tccgatcaac gtggatttat ccctgatgag aaccacgctg
     1861 gcatccgaga tgctgtacgc gatttccaga aaccagaaga agggaattct ggagggacgc
     1921 atttttgagc ttggaaatgt ctttattcca aaggcactgc cgctcacgga atacccggat
     1981 gagagagaga ccctctgtgc gggcgttttc ggagaaaagg aatccttcta tacactcaag
     2041 ggtctggcgg agacggttgc ggatgcatta aacgtaagct ttacctatga ggcggcggag
     2101 agaacctttt tgcacccgta ccagacggcg gagatcttct gtgagggaga gcgcgtcggt
     2161 tatctcggaa agctttccta tgagattcag gatgaacttg acatgcgtgt gcctgcctat
     2221 gtgatggaga ttgacctcgc ggcgcttaag aaatggtatg gcaaagagca gattttcact
     2281 ccgcttccga agtttgcgga ggaaaagcgc gattttgcgt ttgtggtaga taaaaatatc
     2341 acctgtgcac agattgagaa cggcatccgg gaagcctgca aatacgtgac agatgtgaat
     2401 ctgttcgatg tgtatgaagg catccagctt ggaccggaca aaaagagcat ggcattctct
     2461 gttgtattta ccccgcagga ggaagagctc acgggcgaga tggtagaagg atttgtgaag
     2521 aagattttga aacatctcga agcaacactg gatatcagac ttcgtgcgta agaggagtag
     2581 ttatggacgt aaaagatttt tattatgacc ttccgcagga actgatcgca caggatccgc
     2641 tggaggaccg ctcaagctcc agactgatgg tgcttgacaa ggcaaccgga gaggtggaac
     2701 accgccattt taaggatatt gtggactatc taagaccggg tgactgcctt gtcatcaaca
     2761 atacaaaagt aattcctgcc agactgtatg gcgtgaagga aggcactgag gcaaagatcg
     2821 agattcttct gctcaagaga aaagaaaacg atgtctggga gaccctggtc aaaccgggaa
     2881 aaaaatgcaa gatcggaacc agaatcaatt tcggcgaggg acttctgacc ggcgaggtca
     2941 ttgacattgt ggaggagggc aaccgcctga tccagttcca ttacgaaggg atttttgagg
     3001 agattctgga tcagctcgga cagatgccgc tgccgccgta catcacacac cagctgaagg
     3061 acaagaaccg ctaccagacg gtgtatgcaa agtatgacgg ttccgcggcg gcaccgacgg
     3121 cgggactgca ttttacaccg gagcttttag agcaggtgcg cgccatgggc gtggagatcg
     3181 cggaggtgac gcttcatgtg ggacttggca ccttccgccc ggtcaaggag agcgatgtgt
     3241 taaagcatca catgcattcc gagttctacc ggatcgagca gtcggaggcg gacaagatca
     3301 accgggcaaa acaggcggga caccgtatta tcgccgtggg aacgacgagc accagaaccc
     3361 tggagtctgc cgcagatgaa cacggcatgc ttcatgagac gagcggatgg acagagattt
     3421 ttatctaccc aggctatcag ttcaaggtga tcgatgcttt gattacgaat ttccatctgc
     3481 cggagtcgac gctggtgatg ttagtgtctg cgcttgcagg aagggaacat gtgctgcatg
     3541 catatgagat tgcagttcag gagcgttacc ggtttttcag ctttggtgat gcgatgttta
     3601 ttaccgcaaa ataggaggag ttggttccat gctggaaaag agaaaacata agcgccttaa
     3661 gatggaacac gcgctggaac tggcgcggtt aaatgcggac ggcactgccg acgacggcgt
     3721 tgtgaatgcg gatatcctga atatctcacg cggcgggatc ggatttttgt cccgcaagag
     3781 cctcgaggtc ggcagttatt atgacacaag gatcagcttg ttcagccggg agatgattga
     3841 cgctgtgctg gaaatcgtac atgtggaaga gcaggaaaag ggctattac
//
LOCUS       DBMDQJ010000163         3872 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8712_length_3872_cov_1.502254, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000163 DBMDQJ010000000
VERSION     DBMDQJ010000163.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8712_length_3872_cov_1.502254"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="ACZO9R_04510"
     CDS             complement(<1..215)
                     /locus_tag="ACZO9R_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR0912363.1"
                     /translation="MKEKTYVEDIDRSIYDVKDEERDAYKVDEGLTEAIVEQISREKK
                     DPPWMELFRLQALQIYNEMPVPDWGPS"
     gene            complement(212..943)
                     /gene="sufC"
                     /locus_tag="ACZO9R_04515"
     CDS             complement(212..943)
                     /gene="sufC"
                     /locus_tag="ACZO9R_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR0912364.1"
                     /translation="MEDKVILHEVGLTIKKGETHVLMGPNGAGKSTLGYVLTGNPKYS
                     VTEGKILFKGNDITEEAADKRAKAGMFLSFQEPLEVPGLSLESFIRSALSEQGSNKMR
                     VWNFRKELKVAMEVLHMDPSYAGRDLNVGFSGGEKKKSEILQLMMLKPDLAILDETDS
                     GLDVDAVRLASEGIREYQKENPDGALLIITHSTRILDALHVDATHIMVNGHIVASGDA
                     SLVDKINREGYEEYEKLAQKGEDER"
     assembly_gap    945..954
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1526..1762)
                     /locus_tag="ACZO9R_04520"
     CDS             complement(1526..1762)
                     /locus_tag="ACZO9R_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912365.1"
                     /translation="MGRYYRLSKKLTEDESREVQREMREIADVEDVSIKADERMLFVV
                     TKDGQFSDVMSRAVNICSRVANGAELSFARFAEA"
     gene            1974..3647
                     /gene="hcp"
                     /locus_tag="ACZO9R_04525"
                     /gene_synonym="priS"
     CDS             1974..3647
                     /gene="hcp"
                     /locus_tag="ACZO9R_04525"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HIR0912366.1"
                     /translation="MGNNMELDYEMFCYQCEQTANQKGCTKLGVCGKTPEVANLQDLL
                     IFQIKGISCYAKPLIEKGTPMDKSVVSFIENVLFTTLTNVNFDAEVHVELLRESQRIK
                     EELRAYAGAPSQPTLHATYDLPQTKTEMLRDAPIAGIMYDKSLDPDIRSLRQTVLYGL
                     KGISAYGHQARELGYYSDEADDFYILALEALTDDRLSVEELIRLTMRTGEMAIEVMKK
                     LDEANTAIYQNPAPQKVNVHLKKGPFIIVSGHDLKDLEMLLKQTEGTGIHIYTHGEML
                     PCHGYPGLNKYPHLAGNFGGAWQDQQKQFDNLPGCILMTTNCLMRPRDSYKDRIYSTN
                     VVGWDGVKHIGKNENGEKDFSAIIEQALELGGYPEDQDVQEILVGFGHHATLGYADAI
                     VDAVKSGKLRHFFLIGGCDGARPGRNYYTEFAEMVPQDCLIMTLACGKYRFNKLDFGE
                     VAGLPRLLDIGQCNDVYSAIRIATALADAFDTDVNGLPLSLIISWYEQKAVADLLALL
                     SLGIHDIYLGPTLPAFLSPNVLQYLTDTFHLKSISNARDDIRTCLGQAV"
BASE COUNT          892 a         1127 c          864 g          979 t
ORIGIN      
        1 atcgacggtc cccagtccgg caccggcatc tcattgtaaa tctgcagcgc ctgaagccgg
       61 aacagttcca tccacggcgg atcctttttt tcacgggaga tctgctcgac gatcgcctcg
      121 gtcagtccct catcgacctt gtaagcatcc cgttcctcat ccttcacatc gtatatgctg
      181 cggtcaatgt cttctacgta tgtcttctct ttcaccgctc atcctctcct ttttgtgcca
      241 gcttctcata ctcctcgtaa ccttcgcggt tgatcttatc caccagcgat gcatccccgg
      301 atgccacaat atgcccgttc accatgatat gggttgcgtc cacatgaagc gcgtccagga
      361 tgcgcgtgct gtgcgtgatg attaaaagtg cgccgtccgg attctccttc tggtactcgc
      421 ggattccctc ggacgcaagc cgcaccgcat ccacgtccag cccggagtct gtctcgtcta
      481 aaatggcaag atccggtttt aacatcatca actgcaggat ctcggatttc tttttctcac
      541 cgccggaaaa tcccacgttt aaatcccgcc cggcgtagga gggatccatg tgcagcactt
      601 ccattgccac ttttaattct tttctgaaat tccagacgcg cattttattg ctgccctgct
      661 cgctcaatgc actgcggata aagctctcga gggagagtcc cggcacctcg agcggttcct
      721 gaaacgacag gaacattccc gccttggcgc gcttgtctgc cgcctcctcc gtgatgtcat
      781 ttcccttgaa aaggattttt ccctctgtga cgctgtattt gggattgccg gtcagaacat
      841 accccagtgt cgactttccc gcaccgttcg gtcccatcag cacatgcgtc tctccttttt
      901 ttatcgtaag cccgacctcg tgcaggatca ccttatcctc cacgnnnnnn nnnnagccat
      961 agctgcctcc gtttctgaag cgttttctgc agccggaatc ctctttcatg attccctccg
     1021 ctgccctttt ggttttcttt gcgacgctcc cacacatctt tcatatccta gttgcaaggt
     1081 aggatatgat tcaaccacat tctagaacga aaccgttaag aaagcaagag agaaattgcc
     1141 ccgctattga gattgtttct catattcaca gagagcgtgc gatcgcattc cggatagaag
     1201 caggcgggga aattggttgg agcaagatgc gggaatttct tccctgccgc tttttatatc
     1261 caattccgtg tctttccacg tttcgacaat ccacaggatg tgtccgacaa gccgcagaca
     1321 atacgcggac tttttagtgt tcctaacatc accgcttcac agacaatata aaaccgcgcg
     1381 aaccgaaact cgttggcatc tgctattgaa agcatggcag atgccgactc agacatcggc
     1441 ttcgcgcggt tatgttttgt tcattagtga tgtaaggaac acacaaaagt ccttaaattg
     1501 tctgtcagca cgctctcttc cgaatctatg cctccgcaaa acgtgcgaat gaaagttccg
     1561 ctccgtttgc cacgcggctg cagatattga ctgcccggct catcacatcc gaaaactgtc
     1621 cgtcctttgt gacgacaaaa agcatccgct cgtccgcttt gatcgagacg tcctccacgt
     1681 cagcgatttc tctcatctcc cgctgtactt ccctgctctc atcctctgtc agcttttttg
     1741 ataatcgata atatcttccc ataatgccat cctttctgat cgttgcatgt ttggggttag
     1801 ttatatctaa tcctgttttt attatagcac tgcgatctga aatgaaaacg gggttgctga
     1861 taagttttct cattatatcc ggctctgccg catctttttt acatattctg cacatactgt
     1921 gcctgtcgca acacagatta cccggtatat caataaaaat aggagaaatc aacatgggaa
     1981 ataatatgga actcgattac gaaatgttct gctaccagtg tgagcagact gctaaccaga
     2041 aaggctgcac caagctcggt gtatgcggaa aaactcccga agtcgcaaat ctgcaggatc
     2101 ttttaatctt tcagatcaag ggcatcagct gctatgccaa gcctctgatc gaaaaaggca
     2161 ctcctatgga taaaagtgtt gtcagcttca tcgaaaatgt gctgtttacc acacttacga
     2221 acgttaattt tgacgcggaa gtacatgtcg aacttttgcg cgaatcccag cgcatcaaag
     2281 aagaactccg ggcttacgca ggcgctccca gtcagcccac gcttcacgcc acctacgatc
     2341 ttccgcagac caaaaccgag atgctgcgcg acgcgcccat cgccggtatc atgtatgaca
     2401 agtcgctcga tccggacatc cggtccctgc gtcagaccgt tctctacgga ctcaaaggca
     2461 tcagcgccta cgggcatcag gcgagagagc tcggctatta cagcgatgag gcggatgatt
     2521 tctatattct cgccctggaa gccctcacgg acgaccggct gtcggtcgag gaactgatcc
     2581 gccttaccat gcgcaccggt gagatggcaa tcgaagtcat gaaaaaactg gacgaagcca
     2641 acacggccat ctatcagaac ccagccccgc aaaaagtcaa tgtccacctg aaaaaaggtc
     2701 cgtttatcat cgtctccgga cacgatttaa aagaccttga aatgctttta aagcagacgg
     2761 agggaaccgg catccatatc tacacccacg gagagatgct tccctgtcac ggttatccgg
     2821 ggctgaacaa atatccgcac ctcgccggga atttcggcgg tgcctggcag gatcagcaga
     2881 aacagttcga caatcttccc ggctgtattc tcatgaccac caactgcctg atgcgcccgc
     2941 gcgactccta caaagaccgc atctacagca caaatgtggt cggctgggac ggtgtgaagc
     3001 acattggcaa aaacgagaac ggcgaaaagg attttagcgc catcatagag caggcactgg
     3061 aactcggcgg ctatcccgaa gaccaggacg tacaggaaat cctcgtcggc ttcggtcacc
     3121 acgccacact gggctatgct gatgcgatcg tggatgccgt aaaatccgga aaactgcgcc
     3181 atttcttcct catcggcggc tgcgacggag cgcgccccgg acgcaactat tacaccgagt
     3241 tcgccgagat ggttccacag gactgcctca ttatgaccct cgcctgcggc aagtaccgct
     3301 tcaacaagct ggacttcggg gaggtggcgg gtcttccaag actcctcgat atcggacagt
     3361 gcaacgatgt ctactctgcc atccgcattg ccaccgcact tgccgacgcc ttcgacacgg
     3421 acgtcaacgg tcttccgctc tcgctgatta tttcctggta tgagcagaaa gccgtcgctg
     3481 atcttctcgc tctgttaagt ctcggcatcc atgacattta tctcggtccc actctgcccg
     3541 cctttttgag tccgaatgtc ctgcagtacc tgacagacac gttccactta aaatccatca
     3601 gcaacgccag ggacgacatc cgcacctgcc tcggacaggc agtctgattt ccgcagcctt
     3661 aaccgggttg ttgaattata ttctgattat atactatatc cttctgtcac tacagcgtct
     3721 ctccctccag gtagcgatga ggtaaaatcc gctgactgtc tcctgttatc tgctccactt
     3781 ccgggtcagc gtctatcatg gcaaaaagca tctcaacggc actttttgcc acctcatcca
     3841 ccacattgga gatcgtggaa agctgcggtg tc
//
LOCUS       DBMDQJ010000164         3862 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8729_length_3862_cov_2.025525, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000164 DBMDQJ010000000
VERSION     DBMDQJ010000164.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8729_length_3862_cov_2.025525"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(224..679)
                     /locus_tag="ACZO9R_04530"
     CDS             complement(224..679)
                     /locus_tag="ACZO9R_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HIR0912367.1"
                     /translation="MVLSEKETAAIKDLQTQEKTCVEKYSRYSKEAKDPVLVNLFTDL
                     HKKEQQHFDSLGQVLNGTVPNCNCNDSDGKNYNPTATYSLAESEDKKNDCFLATDCIG
                     TEKLVSSEYNTDVFVFADPGVRKLLADIQVEEQNHAEMLYKYKTVNSMS"
     gene            1194..2420
                     /gene="tyrS"
                     /locus_tag="ACZO9R_04535"
     CDS             1194..2420
                     /gene="tyrS"
                     /locus_tag="ACZO9R_04535"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR0912368.1"
                     /translation="MTLYDELVARGLIAQVTDEEEIKELINNGKATFYIGFDPTADSL
                     HVGHFMALCLMKRLQQAGNKPIALIGGGTAMIGDPSGRTDMRQMMTPETIQHNCDCFK
                     KQMSRFIDFSDGKALMVNNADWLMDLNYIDVLREVGAHFSVNRMLTAECYKQRMEKGL
                     SFLEFNYMIMQSYDFYTLFQKYGCNMEFGGDDQWSNMLGGTELIRRKLGKDAYAMTIN
                     LLLNSEGKKMGKTQSGAVWLDPEKTSPFDFFQYWRNVSDSDVLKCIRMLTFLPLEEID
                     AMESWEGAQLNQAKEILAFELTKLVHGEEEATKAREASHALFAGGGDSAHMPTVELSA
                     ADFADGDLDILALLVKTELAPSRSDARRAVEQGGVSVADAKVTDIKTTYSADSFGADG
                     LVVKRGKKKFVKVLVK"
     gene            complement(3041..>3862)
                     /locus_tag="ACZO9R_04540"
     CDS             complement(3041..>3862)
                     /locus_tag="ACZO9R_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR0912369.1"
                     /translation="LIIAREDTNLEDLIVVSYPFVYATEEIADCIEWIKDYSEDSIPV
                     LDEENRIIGVITSQDIVEVVDDEMGEDYAMLAGLTAEEDLEEPLKDSIKKRMPWLIVL
                     LGLGLVVSSVVGMFEHVVAELTIVMCFQSLILDMAGNVGTQSLAVTIRVLMDENLTAG
                     QKVHLVFKEARVGLSNGLILGMMSIILIGCYIYFFKSGSLQFAFAVSGCIGAALILAM
                     LISSLVGTLIPMFFHKIHIDPAVASGPLITTVNDLVAVVTYYGLAWFFLIEMLHY"
BASE COUNT         1012 a          992 c          922 g          936 t
ORIGIN      
        1 tgattatcat ttttaacaaa agacatatgg atgatctcat aaaatgctgc ctgcatttta
       61 ccaaagaatc caatgttttt acatatcgtg ttaagctttc aaggtacaaa tttatatcaa
      121 aggttataaa ccttttgaca ccctaatcct ataagttaaa aaaagccccg cgtgcggaac
      181 tctttgcacc gggacttctc ttatacatga tactttgtct gctttatgac atgctgttga
      241 cagtcttata cttatagagc atctccgcat gattctgctc ctccacttga atgtctgcga
      301 gcagtttgcg gacgcccgga tcggcaaata caaacacgtc ggtattatac tcggaagaca
      361 ccagcttctc ggtaccgata cagtcagtcg caaggaagca gtcgtttttc ttgtcctcgg
      421 actccgcgag agagtaggtg gcggtcgggt tataattctt tccgtcgctg tcgttgcagt
      481 tgcagttcgg tacggtaccg tttaacacct gtcccagcga atcaaaatgc tgctgctcct
      541 tcttatgaag atcggtgaac aaattcacaa gaaccggatc ctttgcctcc ttgctgtatc
      601 tggaatactt ctccacgcag gttttctcct gggtctgaag atccttgatc gccgctgtct
      661 ctttttcact taataccatc gttgttacct cttttcttat ttttttctat gctggtatta
      721 ttgggaaaaa gtttcgcaat tatacacaat atttattttc cggttgacaa atgcgcgcgg
      781 acgcgtctat aatggcgtta atcggagcag aacacaggct ttgacgggaa gaagtaagaa
      841 taagcggaac gaacagaaag caaccggctg atgagaggtg cacggaaagc ttattacgaa
      901 tacatccccg agcttcacac ccaatttgca tgaccgttct ttttgcggcg atgtgatagt
      961 aggctgtgac gtagcggcgc acgttacaag cgcctgagtg ttgtcggaca ctcgcagagg
     1021 cagaccatgt gaatggtctg tgaacaaagg tggtaacacg agtttatacc ctcgtccttt
     1081 ttgaaggacg ggggtttttt gtgcggcagc aaaggtccgg ctccgtccgg ctgttgtacc
     1141 gccgccagaa aaagacaacc acaaatacaa ccacaaagaa aggaactgac atcatgacat
     1201 tatacgatga attagttgcc cgcggcttaa tcgcgcaggt aacagacgaa gaagagatca
     1261 aagaactgat caacaacgga aaagcaacct tctacattgg gtttgacccg actgcagaca
     1321 gtctgcatgt cggacatttc atggcgctct gcttaatgaa gcgcctccag caggcaggca
     1381 acaagccgat cgccttaatc ggcggcggta ctgccatgat cggtgatccg tccggccgta
     1441 ccgacatgcg ccagatgatg acaccggaga ccatccagca caactgcgac tgcttcaaga
     1501 agcagatgag ccgctttatt gatttttccg acggcaaagc gctcatggtc aacaacgcag
     1561 actggctgat ggacttaaat tacattgatg ttctgcgcga ggtcggcgct catttctccg
     1621 taaaccgcat gctgaccgct gagtgctaca agcagcgtat ggaaaaaggc ttaagcttcc
     1681 ttgagttcaa ctacatgatc atgcagagct acgatttcta caccttattc cagaaatacg
     1741 gctgcaacat ggagttcggc ggcgacgacc agtggagcaa catgctcggc ggtacagagc
     1801 tgatccgccg taagctcgga aaagacgctt acgccatgac gatcaacctg ctccttaatt
     1861 ccgagggcaa gaagatgggc aagacacagt ccggcgccgt atggcttgat ccggagaaga
     1921 cttccccgtt tgatttcttc cagtactgga gaaacgtgtc tgactccgat gtcttaaagt
     1981 gcatccgtat gctgaccttc ctccctctcg aggagatcga cgcgatggag agctgggaag
     2041 gcgcgcaatt aaaccaggca aaagagatct tagctttcga gctgaccaag ctggtacacg
     2101 gcgaggagga agccaccaag gcacgcgagg catcccatgc actctttgca ggcggcggag
     2161 attccgcaca catgccgacc gtagagcttt cggctgctga ctttgcagac ggcgatctcg
     2221 acatccttgc acttctcgtg aagacagagc ttgctccatc ccgctccgat gcaagacgtg
     2281 ccgttgagca gggcggcgta agcgttgccg atgcaaaggt caccgatatc aagaccacct
     2341 acagcgctga ttccttcggt gctgacggtc ttgtcgtaaa acgcggcaag aaaaaattcg
     2401 tcaaggttct tgtaaaataa accgtgcatc aacgcaaaaa gaaattgccg cgataagcat
     2461 agaccgcttt tggggtgata agtaatatta ttagaagacc acaccgtggg atttcagatg
     2521 gaaatgcaga tgaacattcg gttcaaaaaa tgtggcattt ctaaccgccc gaatgtctgc
     2581 atccgcgatg atccgtgtct ttccaagttt ggagcactcc gcgggtcatg tccgcatccc
     2641 ggaagacaat ttgtaggact tttgtgtgtg tccctttatg gcacctctga acaaaacata
     2701 accgcgcgaa gccgatgtct gaattgccat ctgccatgtt tttaatagca gatggcaacg
     2761 agtttcggtt cgcgcggttt tatattgtct gtgaagaggt gccattaggg acaccagaaa
     2821 gtccgcccat tgtctccggt gtgtcggaca tgcacccgtc ggattgtcca aacgtggaag
     2881 agacacgaaa tccgatgcag acattcagcc ggaagaaatg ctcacatttt tctccaacga
     2941 atgtccatct gcatttccac atgaaatccc caagtgtgtt tctaataata ttacttatca
     3001 gcgcaaagca cgatatgctt atcgcggcaa tttcctttta ctaatagtgc agcatctcga
     3061 tcaaaaagaa ccatgcaagc ccgtagtatg tcactaccgc caccagatca ttgaccgtcg
     3121 tgatgagcgg tccggatgca acggcaggat caatatgaat tttgtggaaa aacatcggga
     3181 tcagcgttcc caccaggctt gagatcagca tggcaaggat cagcgccgct ccgatacagc
     3241 cggacaccgc aaaggcgaac tgcagggagc cggacttgaa aaagtaaatg taacacccga
     3301 tcaggataat tgacatcata ccaaggatca gaccgttgct aagtccgacg cgtgcttcct
     3361 taaagaccag gtgcactttc tgcccggcag tgagattttc atccatcaac acacggatcg
     3421 tcacagccag ggactgcgtt cccacatttc ccgccatatc gagaatcagc gactgaaagc
     3481 acatgacaat tgtcagttct gcgaccacgt gctcgaacat ccccacaacg gaggacacga
     3541 ccagtcccag tccgagcagc acgatcagcc acggcatacg ctttttgata ctgtctttta
     3601 acggctcttc caaatcttcc tccgcagtca gaccggcgag catggcataa tcctcgccca
     3661 tctcgtcatc gaccacctca acaatgtcct gcgaggtgat gactccgatg atccggttct
     3721 cctcgtccag aactggaatg gaatcttcgg aataatcctt gatccattcg atgcagtccg
     3781 cgatctcctc cgtggcatag acgaacgggt aggacaccac gatcagatcc tccagattcg
     3841 tgtcctcccg cgcgatgatg ag
//
LOCUS       DBMDQJ010000165         3846 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8764_length_3846_cov_1.634980, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000165 DBMDQJ010000000
VERSION     DBMDQJ010000165.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8764_length_3846_cov_1.634980"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(218..1981)
                     /gene="rlmD"
                     /locus_tag="ACZO9R_04545"
     CDS             complement(218..1981)
                     /gene="rlmD"
                     /locus_tag="ACZO9R_04545"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR0912370.1"
                     /translation="MKKKMQINKKKEERRGTQEKMSKVEKTARPAKAEKPIKATRPAN
                     AEKPIKSAERADAVCPYAKKCGGCDYQGMPYEKQLKEKQAYVQKQVGNFCKVLPILGM
                     DEPYHYRNKVHAVFDIEKKRGSRPGARGNGKAANGKQGNGKNGRLAAKPAPGGIISGV
                     YKAGTHEVINIDACQIEDELSSAIIRDIRGLLHSFKIKTYDEDTGYGLLRHVLVRRGF
                     HSGEVMVVLVLGSPVLPSKNHFVKALRELHPEISTVIVNVNDKRTSMVLGDKESVIYG
                     KGYIEDTLCGCTFRISPKSFYQVNPVQTEILYGKAIAYATLTGNETVVDAYCGTGTIG
                     IIAAKSAKKVIGVELNRDAVRDAVKNAKCNNVKNIEFYNADAGQFMVEMAEYRADAKR
                     GEKSGADEVDVVFMDPPRAGSDEAFLSSVVTLAPKRVVYVSCNPETLARDLLYLTKHG
                     YRAQECQPVDMFPWTKHVETVVLLSKGAKGPVDLCSARTEVERRLVDSRKVKVDFSLE
                     NMDLSEFKGKATYEQIKAYVLEKTGLKVSSLYTAQIKKKCGLDVGENFNPAKSENARQ
                     PQCTPEKEDAIMQAFRHFGII"
     gene            complement(1988..2539)
                     /locus_tag="ACZO9R_04550"
     CDS             complement(1988..2539)
                     /locus_tag="ACZO9R_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HIR0912371.1"
                     /translation="MLKLEKGRPADCAGRLEKEVRTYDFLDKLNIEYERIDHEAAMTM
                     EECADVDEALGAMICKNLFLCNRQETTFYLLMIPGDKKFKTKDLSAQIHSSRLSFAKE
                     TYMEELLDITPGSVSVLGLMNDREHRVQLLVDRDVLEGDYIGCHPCINTSSLRIRQKD
                     MWEKIVPALEHEPVFVTLKEPQE"
     gene            complement(2568..3824)
                     /locus_tag="ACZO9R_04555"
     CDS             complement(2568..3824)
                     /locus_tag="ACZO9R_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0912372.1"
                     /translation="MNEKQNLRLKRNVGLDYVHTFLSNMNMQSSIWVLYLAYCGLHLA
                     QIGLLEGIYHATSIVCEIPSGASADLWGRKRCMVLSNLCIAVSCVIMLFSKSFGWFAV
                     SFAVQALGNNLNSGSEEALVYDSMKCLGQEEHYMGVYGRLNVLIEVAQGLATVAGGVL
                     AEFSYFWCYAACVVIAVLALLPVLWMAEPPQCGKAADTAGGSAKDAPAEGTPAQRTIW
                     ETVKHHFAVSACILQKDARVGKIIGFYSVVFAAHTLLFFYSQQYFADFGYNKVQISVI
                     MLLVGGASCLGALASAKMYRRFGKRCLIPETVIIAAAILCYGSGKAGVAAAALMCAGF
                     ANAALYPVQSDSLNALIPSEQRATLISVDSMCFSIAMIVLFPLAGAFADRWGLKTVLL
                     LLGAILLLFLLAAFCIEKESFSSDHS"
BASE COUNT          878 a         1155 c          787 g         1026 t
ORIGIN      
        1 tctttttatt gccgatccat attttaagat cagtgctgtc acaacatcca cacatctctc
       61 aaatcagtct taatacatca gtattattac cttgtccctt gaacaattcc atatctgcaa
      121 aaaggacatc ccctgacctg taaaatctac agttccggaa tgtccttttt tccatacttc
      181 actatatttt taacacatga tactgcttat ccacaatcta tatgattcca aaatgtctaa
      241 atgcctgcat aatcgcatct tccttctccg gtgtacactg tggctgtctc gcattctctg
      301 atttagccgg attgaagttc tcaccgacat ctaatccaca cttcttctta atctgcgcgg
      361 tatacagtga tgaaaccttc agtcctgtct tttcaagtac atatgccttt atctgctcat
      421 aagttgcttt tcccttaaat tcagataaat ccatattctc cagagagaag tccaccttca
      481 cttttctgct gtcgaccagt ctccgttcca cttcggtccg cgctgagcaa aggtccaccg
      541 gacctttagc gcccttggaa agcaagacaa ccgtctccac atgtttcgtc catgggaaca
      601 tatcgaccgg ctggcactcc tgtgcccgat agccatgctt tgtgagatac aaaagatccc
      661 gcgccagcgt ctctggatta caggaaacgt agacgacacg cttcggtgcc agcgtcacca
      721 cggaggagag aaacgcctca tcacttccgg cgcgcggcgg atccatgaag acgacatcca
      781 cctcgtctgc tccgctcttc tctccgcgct tcgcatccgc gcggtattcc gccatctcta
      841 ccatgaactg tcccgcgtcc gcgttgtaaa actcaatatt tttgacgttg ttacacttcg
      901 cattttttac cgcatcgcgc accgcatcac ggtttaactc cacaccaatc actttcttcg
      961 cagactttgc agcaataatg ccgatcgttc cggtgccgca gtacgcatcc acaaccgtct
     1021 catttccggt cagcgtcgca tatgcaatgg cctttccgta gaggatctct gtctgcaccg
     1081 gattgacctg ataaaacgac ttcggtgaaa tacggaaggt gcatccgcag agcgtatcct
     1141 cgatataacc cttgccgtag atcacactct ccttgtcgcc cagcaccatg ctggtacgct
     1201 tatcatttac gttgacaatc acggtcgaaa tctccgggtg aagttcccgc agtgccttca
     1261 caaaatgatt ctttgacggc agcaccggcg agccaagcac caggacaacc atcacctcac
     1321 cgctgtggaa tccgcggcgc accaacacgt ggcgtagcag accgtaaccc gtatcctcat
     1381 cgtatgtctt gatcttaaaa gaatgtaaaa gcccccggat atcacggatg atcgcactgg
     1441 atagttcatc ctcgatctga caggcatcaa tattgatgac ctcatgtgtt cccgctttgt
     1501 atacacccga aataatccca cccggtgccg gctttgccgc taatcttccg tttttgccgt
     1561 ttccctgctt tccatttgca gctttaccgt ttccacgggc tccgggacgg ctgccacgct
     1621 ttttttcaat atcaaacacg gcgtgcacct tgttccggta atgatacggc tcgtccatcc
     1681 ccagaatcgg cagcactttg cagaagtttc cgacctgctt ctgcacatat gcctgtttct
     1741 ctttgagctg cttctcatat ggcatgccct gatagtcgca tccaccgcat ttcttcgcgt
     1801 aaggacagac cgcatctgcg cgctccgccg actttatcgg tttttccgca tttgccggtc
     1861 ttgtcgcctt tatcggtttt tccgctttcg ccggtcttgc cgtcttttcc actttcgaca
     1921 ttttttcctg cgttccccgc cgttcttctt tcttcttatt tatctgcatt ttctttttca
     1981 tatctgctca ctcctgtggt tctttcaatg tcacaaagac cggttcgtgc tccagcgccg
     2041 gaacaatttt ttcccacatg tctttctgcc ttatgcgaag gctggacgtg ttgatgcacg
     2101 gatggcaccc gatatagtca ccctccagca catcccggtc caccagaagc tggacacggt
     2161 gttccctgtc gttcatcaga ccgagcacgc tgaccgaccc gggcgtgatg tctaaaagct
     2221 cctccatata agtctctttt gcaaaagaca ggcgcgacga atggatctgt gccgacagat
     2281 cctttgtctt gaacttctta tctcccggta tcatcagcag gtaaaatgtt gtctcctgcc
     2341 ggttgcacag aaacagattc ttgcagatca ttgcgccgag cgcttcgtcg acatccgcac
     2401 actcttccat cgtcattgcc gcctcgtgat cgatccgctc atattcgata ttcagtttgt
     2461 ccagaaaatc gtacgtccgg acttcttttt ccagacgtcc cgcgcagtcg gcaggacgtc
     2521 ccttctctaa ttttaacata tgcttacctc gcttttttca tcatacatca ggaatgatcg
     2581 cttgaaaagc tttctttttc gatgcaaaag gctgccaaaa gaaacagcag taaaatggca
     2641 ccaagcagca gcaacaccgt ctttaacccc caccggtccg caaacgctcc tgcgagcggg
     2701 aacagcacaa tcatggcgat ggaaaaacac atgctgtcga ccgagattaa ggttgccctc
     2761 tgttcggacg gaatcaatgc gttcagggag tcagactgga ccggatacaa cgccgcgttc
     2821 gcaaatccgg cacacataag ggccgctgca gccacgcccg ccttccctga tccgtagcag
     2881 agaattgccg ccgcaatgat aaccgtctcc ggtatcaggc accttttccc aaaccgccgg
     2941 tacatttttg cactggcgag cgcaccaagg caggaagcgc cgcccacgag cagcatgatg
     3001 acactgatct gcactttatt gtagccgaaa tccgcgaaat attgctggct gtagaaaaac
     3061 agcagcgtgt gcgccgcaaa aacgacagaa tagaagccta tgatttttcc gactctggca
     3121 tccttttgca ggatacatgc actgactgca aaatgatgct tcacggtctc ccagatcgtt
     3181 ctctgtgccg gagttccttc cgccggcgca tcttttgcgc tcccgcctgc cgtatcagct
     3241 gccttgccgc actgcggcgg ctccgccatc cacagtaccg gcagcaatgc cagcaccgca
     3301 atcacaacac atgccgcata gcaccagaag taggagaatt ctgccagcac accgcctgcg
     3361 accgttgcaa gcccctgcgc cacttcgatc aacacattga gtcttccata gacacccatg
     3421 tagtgttctt cctgccccag gcacttcatg ctgtcatata caagcgcttc ctccgagcca
     3481 gagtttaaat tatttccaag tgcctgtacg gcaaagctta ccgcaaacca cccgaaactt
     3541 tttgagaaca gcatgatcac gcaggaaacc gcgatgcaga ggttgctcaa aaccatgcag
     3601 cgttttctac cccacagatc ggctgacgcc ccggaaggaa tctcacacac gatgcttgtc
     3661 gcatggtaaa ttccctctaa gagtccgatc tgtgccaggt gcagcccgca atatgcgaga
     3721 tacaacaccc agatcgagct ttgcatgttc atattggaaa gaaacgtatg gacatagtcc
     3781 agtccaacat ttctcttaag tcgaagattc tgtttttcgt tcatctgtaa ttcctctttt
     3841 catttt
//
LOCUS       DBMDQJ010000166         3845 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8766_length_3845_cov_1.433494, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000166 DBMDQJ010000000
VERSION     DBMDQJ010000166.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8766_length_3845_cov_1.433494"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(373..1185)
                     /gene="murI"
                     /locus_tag="ACZO9R_04560"
     CDS             complement(373..1185)
                     /gene="murI"
                     /locus_tag="ACZO9R_04560"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR0912373.1"
                     /translation="MEKYENRQECPIGVFDSGVGGISVLRELVAQMPNENYIFFGDSK
                     NAPYGTKTLEEVQKLTCADAEYLLSRGVKALVVACNTATSAAIRILREKYADMPVIGI
                     EPALKPAVHAGGHPRVLVMATPMTLREEKFHALMQRFESDAEILRLPCPGLVEYVEQG
                     VLEGAELEAFLANLFEPYQSHPVDCVVLGCTHYPFVRETIQKLLGAGVQIFDGGAGTA
                     RETERRLKDCGLCSGRAQQGTVELCNSLASEQMKQLEIRLLQSGTVQAIIKK"
     gene            complement(1188..>3845)
                     /locus_tag="ACZO9R_04565"
     CDS             complement(1188..>3845)
                     /locus_tag="ACZO9R_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR0912374.1"
                     /translation="VKELKNGKLREQKVPAGVELTLPDGTVYPEKRSQTDAKIEELIG
                     LSKEQFTQTAMIAQGDFLKLLYAKSDDRKKIFSKLFHTDAYWRIQENLKRRSFEMDRV
                     LAENERAAEQERARVILPREELKELPLPEAVEQIGTWEKELTASQETVREEIRRLTAA
                     ISKAKEVNELFEGLRACEQQVQKLKEEEPVEARRKEQLEAAVRAERVHVKELRCNERL
                     DERRQSDETVERMKSLIAEEKEAHGRQEQDFNTLKMQSADAAEADAEKMLRIREQLPV
                     YERLGEAVEREQQAKQAYESAKQYYERAVREGASRLLAEKNRQLLLEEKYVSAAKAWE
                     RASARSTEASAEYERVYQLFLKEQAGILAQNLREAEPCPVCGSLTHPHPASLAENAIS
                     EAAVKAAKEQREQAETARDTAYQEFDRQKSAAAEQELRLEQAKQQFLALAGDACGDGE
                     KELYTFAALQAAEEPAAKKRSLTERGRQAERFSREGKKHPGETGEAVDRLKLESLKRD
                     WEEGQRETARIRAELSYETKERAKEALQQLTDEADRRTKRLADAEETLQKKKEDISRQ
                     EGMLTQEQTKNAGFVQECLELEKEYRQALRDAGFSSEEAYHEAFLTESGRETLVQQSE
                     AYVRNRVESEGRLLTMQKLTEGKQEIGILAWETELEAQKQRQQELHEEILAMHTAYET
                     DAKVVERCREYFAAHERLDAENQVLKRLYCTADGRLAGSAKIDFETYVQRRYFRQVIY
                     EANRRLLTMSGHQFMLKLKETENSGRKSNEGLDLSVYSLVTDSERDIRTLSGGESFLA
                     ALAMALGLSDIAIRKAGAVHLDMMFIDEGFGTLDAQSRKQAIEVLSQLAGSERLVGII
                     SHVTELKEQIEHRLYVTRTENGSEAVWEE"
BASE COUNT          710 a         1278 c          827 g         1030 t
ORIGIN      
        1 ttctaatatg aatctgtcaa gattcattat atcacaagca tagtaagttc aacatataaa
       61 aactgcattt tacattcttt gtatgttccc cccaatgaaa aacccccggg acatctctgt
      121 cccgggggtt tttcgaggag tttagttatg aaaatgttaa tctatataaa ggaatcttca
      181 ccgaaaggtt ctgcagaaat atgaccttta ttccacatct ctgagctgct gggaacgaag
      241 ctctgggaaa ccgtcctcag ctcctttcga tgatgttagt atacggaaaa aatgtgtccg
      301 aagtatgtcg attttcataa agctttctaa aaaaacgttc aatgttattt agatttcttt
      361 aatattttat ttctattttt ttataattgc ctgcaccgtg cccgactgga gcagccggat
      421 ctccagctgt ttcatctgct ccgacgcaag actgttgcac aattccacgg ttccttgctg
      481 cgctctgcca ctgcacagac cgcaatcctt aagcctgcgt tctgtctccc gcgcggtccc
      541 cgctccgccg tcaaagatct gcactccggc tccgagaagc ttctggattg tctcccgcac
      601 aaacggataa tgcgtgcagc caagcacgac acaatccacc ggatggcttt ggtacggttc
      661 aaacagattt gcaagaaacg cttcgagttc tgcgccctcc agcacgccct gctccacata
      721 ctccaccagt cccgggcacg gcagccttaa gatctcggca tccgactcaa accgctgcat
      781 cagggcatga aacttctcct cgcgcagggt catcggcgtt gccatgacaa gaacgcgcgg
      841 gtgaccgccc gcgtgcaccg ccggctttag tgccggctca attccgatca ccggcatatc
      901 cgcatatttt tcccgaagga tccggatggc cgcgcttgtc gccgtgttgc atgccaccac
      961 gagcgccttt acgccgcgtg acagcagata ttcggcatcc gcacaggtca gcttctgcac
     1021 ttcctccagc gtctttgtac cgtagggcgc attcttagaa tccccaaaaa agatataatt
     1081 ctcattcggc atctgtgcca ccagttcccg cagcacacta attcctccga ctcccgaatc
     1141 aaacacaccg atcggacatt cctgtctgtt ttcatacttt tccactgcta ttcctcccag
     1201 actgcctcgc ttccgttctc cgtgcgcgtg acataaagtc tgtgttctat ctgttctttt
     1261 aattctgtca catgggagat gattcccacc aggcgctcgc ttcccgcaag ctgtgacaat
     1321 acttcaatcg cctgttttct ggactgcgca tccagtgtac cgaatccctc atcgataaac
     1381 atcatgtcca gatgcacggc acccgccttg cggatggcaa tatccgaaag tcccagcgcc
     1441 atggcaagcg ctgccagaaa cgactcgccg ccggataagg tacggatatc gcgctcactg
     1501 tccgtgacca gactgtacac cgacagatcc agaccttcgt tggatttgcg tccgctgttt
     1561 tccgtctctt ttagttttag cataaactga tgtccgctca tcgtcaatag ccgtctgttc
     1621 gcctcgtaga tcacctgccg gaaataccgg cgctgcacat acgtctcaaa atcaatcttc
     1681 gccgagcccg ccagacgccc gtccgccgtg cagtatagtc ttttcagcac ctgattttcc
     1741 gcatcgagcc gctcgtgtgc ggcgaaatat tccctgcacc gctccaccac tttcgcatcc
     1801 gtctcgtatg cggtgtgcat tgcaagaatc tcctcgtgaa gctcctgctg gcgctgtttt
     1861 tgcgcctcca gttccgtctc ccatgccaag atcccgatct cctgctttcc ctcggtcagc
     1921 ttctgcatag tcagaagcct gccctcactt tccacgcgat tccggacgta tgcctcggac
     1981 tgctgcacga gcgtctcccg cccgctctct gtaaggaatg cttcgtggta tgcctcctcc
     2041 gaagaaaatc cggcatcacg cagcgcctgc cggtattctt tctccaattc cagacactcc
     2101 tgcacaaatc cggcattctt cgtctgctcc tgcgtcagca ttccctcttg tctggaaatg
     2161 tcttcctttt tcttttgcag tgtttcctct gcatcagcaa ggcgcttcgt acgccggtct
     2221 gcctcatccg tcagctgctg cagcgcttcc ttcgcccgct ctttcgtctc ataggataac
     2281 tccgcgcgga tccgtgccgt ttcccgctgg ccctcttccc agtcgcgctt gagcgactca
     2341 agcttcaggc ggtccaccgc ctctcccgtt tccccgggat gttttttccc ctcccgtgaa
     2401 aatcgctccg cctgccgtcc acgctctgtc agagacctct tttttgctgc aggttcttct
     2461 gccgcctgca gtgcggcaaa cgtataaagt tctttctctc cgtcgccgca cgcatctccc
     2521 gcaagcgcca gaaactgctg cttcgcctgc tccagacgca gctcctgctc tgccgccgcg
     2581 cttttctgcc ggtcaaattc ctgatacgcc gtatcgcgcg ccgtctccgc ctgctcccgc
     2641 tgttccttcg ccgcctttac cgccgcttcg ctgatcgcat tctcagcaag ggacgccgga
     2701 tgcggatgtg tcagggagcc gcagaccgga caaggctctg cctcccgcag attctgtgcc
     2761 agaatgcccg cctgttcctt caaaaaaagc tggtacacgc gctcatattc cgcagacgcc
     2821 tccgtggacc gggcggacgc ccgctcccat gcttttgcgg cactgacata cttttcctcc
     2881 agtaaaagct gcctgttttt ttccgcaagc agccggctgg cgccttcccg caccgcacgc
     2941 tcataatact gcttcgccga ctcgtacgcc tgctttgcct gctgttcccg ctctactgcc
     3001 tctcccaggc gctcatacac cggaagctgt tcccggatgc ggagcatttt ctccgcgtcg
     3061 gcttccgccg catcggcgct ctgcatctta agcgtattaa agtcctgctc ctgcctgcca
     3121 tgtgcttcct tttcctccgc gataaggctc ttcatccgct ccaccgtctc gtcggactgc
     3181 cgccgctcat ccagacgctc attgcagcgc aattccttca catgcaccct ctccgcacgt
     3241 actgccgcct ccagctgttc ttttcggcgc gcttccacgg gctcttcttc cttcagcttc
     3301 tgtacctgct gttcacaggc tcttaatccc tcaaacagtt cattgacttc cttcgcctta
     3361 gagattgccg cagtcagtct ccggatctcc tcccgcacgg tctcctgcga agcggtcagc
     3421 tccttctccc aagtaccgat ctgctccacc gcttccggaa gcggaagttc tttcagttct
     3481 tcgcgcggca gaatcacgcg cgccctctcc tgctcggcag cccgctcatt ttccgcaagc
     3541 acccggtcca tctcgaatga acgccgcttt aagttctcct ggattctcca gtatgcgtcc
     3601 gtgtgaaata atttggagaa aatttttttc cggtcgtccg actttgcata cagaagtttt
     3661 aaaaaatccc cctgcgcgat catggcggtc tgtgtgaact gttccttcga cagcccgatc
     3721 agctcttcga tcttcgcatc ggtctggctc cttttttccg gatacaccgt tccgtccggc
     3781 agcgtcagtt ccacgcctgc cggaaccttc tgctcccgca attttccgtt tttcaattct
     3841 tttac
//
LOCUS       DBMDQJ010000167         3839 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8784_length_3839_cov_1.384965, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000167 DBMDQJ010000000
VERSION     DBMDQJ010000167.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8784_length_3839_cov_1.384965"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            33..1109
                     /locus_tag="ACZO9R_04570"
     CDS             33..1109
                     /locus_tag="ACZO9R_04570"
                     /inference="COORDINATES: protein motif:HMM:NF024878.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR0912375.1"
                     /translation="MPCLRMSVNRLREGMMIKEDVFSKTGAVIVPDGAVVTKDVVALL
                     TRHFVDSVMVEYPTGRKERPEQQEQSAPAKEQHLKEFQAEFSVAEQSISEELKDIVYR
                     SKDVNISMLLEPLNGLLKKADDDTDLADMLLHMKKQQSGLYAHTINVALFGQLLARWS
                     GCTEAEMEEVMAAGLLHDIGFLELWKDGQEQTEFLQEYETERYEKHVVSGYNLIKKMN
                     IDQRIKQAVLTHHERVNGSGFPLQVMGDNINWISRILAVADTYDALTMRQGDKAGISA
                     FEAIKKMEDEGYNRLDANYLMTFLKRIAETMIQRNVILNDGRMGRVVMINKYKLSCPL
                     VQVGDTFVDLAKQSRYYIQEILEE"
     gene            1188..1964
                     /locus_tag="ACZO9R_04575"
     CDS             1188..1964
                     /locus_tag="ACZO9R_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HIR0912376.1"
                     /translation="MNGITSMQRSSTSYADYGKFASGKKVNRAADGAAELAIIQKQET
                     QTRSYKVGENNIGAAKDMLNVADGAMSGIADYLQKIRELGVRASNTATITDAERTSIQ
                     TQIEQYKQGISDIAKQTSFNTKPLLDGTSQKYNIMTDATGNGMKITTADATLQALGIA
                     DFDVTKDFDLTQVDDALARVSAGRSGMGAQSNALEYAYRYSTNTRLNVTAGKSRLEDL
                     DYGQAVSEQKKKQTLQQYAFFMQKKQMEQRTRAAANFLVS"
     gene            2246..>3839
                     /locus_tag="ACZO9R_04580"
     CDS             2246..>3839
                     /locus_tag="ACZO9R_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR0912377.1"
                     /translation="MEKNRIRPVKAGKGIRMSYSRQKEVLEMPNLIEVQKDSYQWFLS
                     EGLKEVFDDISPIADYSGHLSLEFVDFTLCEDDVKYTIPECKERDATYAAPLKVKVRL
                     HNKETDEINEHEIFMGDLPLMTETGTFVINGAERVIVSQLVRSPGIYYGIAHDKVGKK
                     LYSCTVIPNRGAWLEYETDSNDVFYVRVDRTRKVPITVLIRALGVGSNQEIVDLFGEE
                     PKILASFGKDVATNYEEGLLELYKKIRPGEPLTVESAESLISAMFFDPRRYDLAKVGR
                     YKFNKKLMLRNRINGHVLAEDVVDVSTGEIIAEAGTEVTRSLADDIQNAAVPYVWIQG
                     ETRNIKVLSSMMVDLRHYVDCNPKDLGITELVYYPVLKQLMDEYPDVEDLKEAIHKNV
                     HDLIPKHITKDDIFASINYNMHLEYGIGHDDDIDHLGNRRIRAVGELLQNQYRIGLSR
                     LERVVRERMTTLDLDGISPQSLINIKPVTAAVKEFFGSSQLSQFMDQNNPLSELTHKR
                     RLSALGPGGLSRDRAGFEVRDVH"
BASE COUNT         1088 a          775 c         1117 g          859 t
ORIGIN      
        1 ggatgaaagt acgggaaaga ggagaagata ccatgccgtg tttgaggatg tcggtcaacc
       61 gcctgcggga gggaatgatg ataaaagagg atgtgttttc aaagacaggg gcggtgattg
      121 tgccggacgg agcggtcgtc acgaaggacg tcgtcgcgct gctgaccaga cattttgtgg
      181 acagtgtgat ggtggaatat ccgacgggca ggaaagaacg cccggaacag caggaacaga
      241 gtgcgccggc gaaggaacag catctgaaag agttccaggc agagttctcg gttgccgaac
      301 agtcgatttc ggaagaactg aaagatattg tataccggag taaggatgtg aacatttcca
      361 tgctgctgga gccgctgaac ggactgctga aaaaagcaga tgatgataca gatcttgcgg
      421 acatgctgct tcatatgaaa aaacagcagt caggtctgta cgcacatacc attaacgtgg
      481 cgctttttgg acagcttctt gcacggtgga gcggatgcac cgaggcggag atggaggagg
      541 tcatggcggc ggggctgctt catgacattg gatttcttga actctggaag gacgggcagg
      601 aacagacgga atttctgcaa gaatacgaga cggaacgata tgagaaacat gtcgtgagcg
      661 gatacaacct tattaagaag atgaacatag accagcggat taaacaggcg gttctgacgc
      721 accacgagcg cgtgaatgga agcgggttcc cactgcaggt aatgggggat aacatcaact
      781 ggatttccag aattcttgcg gtggcagata cttacgatgc ccttacgatg cgtcagggag
      841 ataaagcggg catctcggca tttgaagcga tcaagaagat ggaggatgag gggtataacc
      901 gtctggacgc caactatctg atgacatttc tgaagcggat tgccgagacg atgatccagc
      961 gcaacgtgat cctgaatgat ggcaggatgg gaagagtggt catgattaac aaatataagc
     1021 tgtcatgtcc gcttgtgcag gtgggggaca cttttgtcga tctcgcaaaa caaagccggt
     1081 attacattca ggagatactt gaagagtagc ggtattgtgt gttataaaga aaatagaaaa
     1141 ggcgggcagg gagagcagcg gaacttgaga acagaaagga ggcttttatg aacggtatta
     1201 cttcgatgca gcgaagcagc acatcttatg cagattacgg gaaatttgca agcggcaaaa
     1261 aagtgaaccg ggcggcggac ggtgcggcgg agcttgcgat catccagaag caggagacgc
     1321 agaccagaag ctataaggtc ggggagaaca acattggcgc ggcaaaagat atgctgaatg
     1381 tggcggatgg cgcaatgagc gggattgcgg attatcttca gaagatccgt gaacttggtg
     1441 ttcgggcgtc caatacggcg acgatcacag atgcggagcg gacaagtatt cagacgcaga
     1501 tcgagcagta taagcagggc atttcagata ttgcaaaaca gacaagcttt aacacgaagc
     1561 cgcttctgga tggcacgtcg cagaagtaca atatcatgac ggatgccacc ggcaacggaa
     1621 tgaagattac gactgcagat gccacattac aggcacttgg gattgcggac tttgatgtga
     1681 cgaaagattt tgatctgaca caggtggatg atgcgcttgc cagagtgagt gcagggcgca
     1741 gcggtatggg cgcacagagc aatgcattgg agtatgcgta ccgctacagt accaacacca
     1801 ggctgaatgt aacggcagga aaaagccggc tcgaggatct ggattacgga caggcggtat
     1861 ccgaacagaa aaagaagcag accctgcagc aatatgcatt ttttatgcag aaaaaacaga
     1921 tggaacagag aacccgcgcg gcggcaaatt ttcttgtctc ataagcggtg gcgaacggcg
     1981 gagcgaaggc tttagagctt tccttcgtcg ttttttgcgt actcacggag aaaaaataaa
     2041 tattttaaaa aatccttgac gcggcacttg ctattgtgct atcatatacg ttgcactatt
     2101 gtggtgcatt gggcttattg cgcgcttttc gcgttgtatg ccgccggatg tgcctatacc
     2161 gtatcttgtg gttacggtgg attgagacag ttgctgccac ttttggggca gcttagtgta
     2221 aataaaaacg agaggtgaaa cgtcaatgga gaaaaacaga atacgtccag tgaaagctgg
     2281 aaagggcata cgtatgagtt actcgagaca gaaagaggta ttggaaatgc cgaatctgat
     2341 agaagtccag aaagactcct atcagtggtt cttaagcgag ggcctgaaag aagttttcga
     2401 tgatatctct ccaatcgctg attacagcgg tcatctgagc ctggagtttg tagattttac
     2461 attgtgtgag gacgatgtaa agtacacaat tccggagtgc aaagaaagag atgcaactta
     2521 tgcggcacct ttgaaggtca aggtcagact tcacaacaag gagactgacg agatcaatga
     2581 gcatgagatt ttcatgggcg accttccttt gatgacagag accggtactt tcgtgatcaa
     2641 cggcgcagag cgtgttatcg tcagccagct ggtgcgttcc ccgggtatct actatggaat
     2701 cgctcacgat aaagtgggta aaaagctgta ttcctgtact gtcatcccta accgtggtgc
     2761 gtggttagag tatgagacag actccaatga cgtattttat gtccgtgtag atagaaccag
     2821 aaaggtgccg atcacggtat tgatccgtgc acttggcgtc ggaagcaacc aggagatcgt
     2881 agacctgttc ggtgaggagc cgaagattct tgcaagtttc ggcaaggacg tggcaaccaa
     2941 ctacgaagag ggacttctgg aattatataa gaaaatcaga ccaggcgagc cgctgacagt
     3001 tgagagtgcg gagagcctta tttctgctat gttttttgac ccgagaagat atgatcttgc
     3061 aaaggtcgga cgctataagt ttaataagaa gttaatgctg cgcaaccgta tcaacggaca
     3121 tgttctggca gaggatgttg tcgatgtgtc cacaggcgag atcatcgccg aggcaggaac
     3181 cgaagtgaca agatccctgg cggatgatat tcagaacgcg gctgttccgt atgtatggat
     3241 tcagggcgag accagaaata tcaaggtgct gtcttccatg atggtagatc tgagacatta
     3301 tgtggactgc aacccgaagg atctcggcat cacagagctc gtgtactatc cggtattaaa
     3361 gcagctcatg gatgaatatc cggacgtgga ggatttaaag gaggccattc acaagaatgt
     3421 gcatgacctg attccgaagc atattaccaa ggatgatatt tttgcatcca tcaactataa
     3481 catgcatctg gagtacggca tcggtcatga tgacgatatc gatcatctgg gtaacagacg
     3541 tatccgtgcg gtcggagaac tgttacagaa ccagtaccgt atcggtcttt ccagactgga
     3601 gagagtggtc cgcgagagaa tgaccaccct ggatctggac ggtatttccc cgcagagcct
     3661 gatcaacatt aagccggtaa ccgcagctgt aaaagagttc ttcggatctt cccagctgtc
     3721 ccagtttatg gatcagaaca acccgctgtc agagctgaca cacaagagac gtctttccgc
     3781 attgggaccg ggtggtctgt ccagagaccg tgcaggattc gaggtgcgag atgtacatt
//
LOCUS       DBMDQJ010000168         3830 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8805_length_3830_cov_1.278359, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000168 DBMDQJ010000000
VERSION     DBMDQJ010000168.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3830)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3830)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3830
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8805_length_3830_cov_1.278359"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(329..1423)
                     /locus_tag="ACZO9R_04585"
     CDS             complement(329..1423)
                     /locus_tag="ACZO9R_04585"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0912378.1"
                     /translation="MIKHKDHFHGSDLEKIEEIYGIKKEEIVSFSANVNPLGVSPLLK
                     HTLSDQIDAITSYPDREYTSLRRCIAAYCGTETENVIVGNGSTELISLFIQVEHPKKA
                     LVIGPTYSEYEREIALGGGTTLYYPLREADDFKLNVEDFLSHLNESIDLLVICNPNNP
                     TSSSIPRSDMRHILDVCKQYDIYVMVDETYVEFADNMAELTAVPLTNYYNNIVILRGT
                     SKFFAAPGLRLGYAVTGNRDLIKSINTRKNPWTINSLAVIAGETMFTDSAYIKETKSL
                     ISSERARLCKLFNKNPDFKVYDPSANFVLVRILREDLTSQDLFDRAIRQKMMIRDCST
                     FPFLDNKYIRLCFMRPEDNDRLIRCLFHQN"
     gene            1501..>3830
                     /locus_tag="ACZO9R_04590"
     CDS             1501..>3830
                     /locus_tag="ACZO9R_04590"
                     /inference="COORDINATES: protein motif:HMM:NF040159.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HIR0912379.1"
                     /translation="MRKRIQQLARGKFQYARPLLSFSADKVDIEVTEGRDYTGDFIIT
                     STNHVPFRGVVYTSDSRMECLTPQFEGEEVRIRYQFHSNGLIEGDIVKGDFFLICNQG
                     EYNLSFVVSVSRLYADTSAGRIKNLNDFTGLAKSSAQEAYHLFYSGHFKNILKPSEVR
                     ERLLYDGEADGTASWQKVEEYLRGCRQKKPVAVTLLADHASFEQVAGDRRESLKLHRD
                     EWGYLSISIESDAPFLLPEKSQLTDEDFIGSTLEVPYYVKKAAMHAGKNFGRLSIVLP
                     EQTLTFEVCATTDGAKAVSEEHLELQRGRMRLAQLYIDYRLKKIVTGAWANQSVELLD
                     HMMAMEPECEMYALMKAQALIVNRQKQEASWIMEDYKRGCRDRETPEWGYYLYLCTLI
                     EREPSYVDKLTDEMELLFKKNPKSSLLFWILLFLRESYYYSSPRKYKAIEAWIEGGCS
                     SPYLYLESYYLIWQDPYLLTRLGTYELKVLRWAMRQGVLTKELAQQIRHLLPEVREKS
                     RVLYEILEAAYQVEPEEEMLSAICAYLIRTQCYETEYHRWYELGIEQKIRLTGLYEAY
                     LMSLDAREVGGVPRMIQMYFQYDSTLSYTQKAVLFVNIIAARTKQPEVYQKYQRTMEQ
                     FAMAQIEAGHINDHLAVIYDEMLPKGILDEELAHSLAPLLFTHRISCTNRQIARAIIW
                     HEEMKMPQSVSFVNGTAYFKAYTPQYSIVLEDTNGNRFCSSVVYQDEALMYPERYVGQ
                     CLSLAPGENAYLLWHMSRHMEPEAWSDTEKTYALQLL"
BASE COUNT          959 a          822 c         1100 g          949 t
ORIGIN      
        1 cctctgaaca aaacataacc gcgcgaagcc gatgtctgag tcggcatctg ccttattttc
       61 aatagcagat gccggcgagt ttcggttcgc gcggttttat agtgtctgtg aagaggtgcc
      121 attagggaca ccagaaagtc cgctcattgt ctgcgggttg tcggacacac tccgtgagtg
      181 tccaaacgtg gaagagacac ggaatgggat gccagctgcg gcatggaaga aatacatgaa
      241 ttttgcttca aagaatttgc ccccgtctgt tttattccgg aactttcggt tctcgggtaa
      301 tctcacaata tcacggatcc cgcttttttt aattctgatg gaagaggcag cggatcagac
      361 ggtcgttgtc ctctggtctc ataaagcaga gccggatgta tttattatcc aggaacggaa
      421 acgtcgaaca gtcgcggatc atcattttct gccggatggc gcggtcaaac agatcctgcg
      481 aggtgagatc ttcgcgcagg atgcgcacca gaacaaagtt ggcacttgga tcgtatactt
      541 tgaagtccgg attcttatta aaaagcttac acaggcgggc gcgctcggag gagatcaacg
      601 atttggtctc tttgatgtag gcgctgtcgg tgaacattgt ctcaccggcg atgacagcaa
      661 gagaattgat cgtccacgga ttcttgcgtg tattgatgga cttgatcaga tcgcggttgc
      721 ctgtgacggc gtatccgaga cgcagtcccg gagcggcaaa gaacttggag gtgccgcgga
      781 gaatcacgat gttgttataa taatttgtca gcggaactgc cgtgagttct gccatattat
      841 cggcgaattc cacgtaagtc tcatctacca tgacatagat gtcgtactgc ttgcagacgt
      901 ctaagatgtg gcgcatgtcg ctgcgcggga tcgaggagga agtcgggttg ttgggattgc
      961 agatgacgag aagatcaatg ctctcattta agtgcgagag aaaatcctcg acattgagct
     1021 tgaagtcatc ggcttcccgc agcggataat agagtgtggt gccgccgccc agcgcgattt
     1081 cacgttcgta ctcagagtaa gtaggaccaa tgacaagggc ctttttcgga tgctcgacct
     1141 gaataaaaag ggagatcagc tcggtggagc cgtttccgac aatgacattc tcagtctcgg
     1201 ttccgcagta tgcagcgatg catctgcgca gagaagtgta ctccctgtcc ggatagctgg
     1261 taatggcgtc gatttgatcg gacagcgtgt gttttaacag cggagatact cctaacggat
     1321 tgacattggc ggaaaaactg acaatttctt cctttttaat gccgtagatt tcttctattt
     1381 tctcgagatc acttccatga aaatggtcct tgtgttttat cattgattac tcctccaaat
     1441 tagtttataa tctattatag tcattgcgtt caaaaaagca atagaaaagc aggtgtcaat
     1501 ttgcgtaaac ggatacaaca gctagcaagg ggaaagttcc aatacgccag accgcttctt
     1561 tctttttctg cagataaagt cgacattgag gtgacggagg gtagagatta caccggagat
     1621 ttcattatca caagtacgaa ccatgtgccg tttcggggag tagtctatac ctctgattcc
     1681 agaatggaat gtctgacacc ccagtttgag ggggaagagg tgcggatccg gtaccaattt
     1741 catagcaatg gactgatcga aggggatatt gtcaaggggg atttttttct aatctgcaac
     1801 cagggagaat ataacctttc ttttgttgtg tctgtatcca gattgtatgc ggatacctct
     1861 gcgggacgca ttaagaatct gaacgatttt acgggactgg caaaaagcag tgcccaggag
     1921 gcatatcatc tgttttattc cggtcatttt aaaaatatac taaagccctc cgaggtcagg
     1981 gagcgtcttc tctatgacgg ggaggcggac ggcacggcgt catggcagaa ggtggaggaa
     2041 tatctgcgcg ggtgcagaca gaaaaagccg gttgcggtta cgcttcttgc ggatcatgcc
     2101 tcatttgagc aggtcgccgg cgaccgcagg gagagcctga agctgcaccg ggatgagtgg
     2161 ggctatctct ccatttcgat tgagtcggac gcgccgtttc ttttgccgga aaagagtcag
     2221 ctgacggatg aggattttat cggaagcaca ctggaagtgc catattatgt gaaaaaagct
     2281 gccatgcatg ccgggaagaa ttttggaaga ctttcgattg tcctgccgga gcagacgctt
     2341 acgtttgaag tgtgcgccac gacggacggc gcaaaagccg tatcggagga gcatttggaa
     2401 ttacagcgcg gacggatgcg gcttgcacag ctatatattg attaccggct gaaaaagatt
     2461 gtgaccggcg catgggcgaa tcagtccgtg gagctcctgg atcatatgat ggcaatggaa
     2521 ccggagtgtg agatgtatgc gctgatgaaa gcgcaggcgt tgattgtgaa tcgacagaag
     2581 caggaagctt cgtggattat ggaggattat aagcgcggct gcagggacag ggagacgccg
     2641 gaatggggat actatctgta tctgtgcaca ctgattgaga gggaaccgtc ctatgtcgat
     2701 aagctgacgg atgagatgga actccttttt aagaagaatc cgaagagttc gctgttgttc
     2761 tggattcttt tgtttttgcg ggaatcctac tattacagca gtccaagaaa gtataaggcg
     2821 attgaagcat ggatcgaggg cggatgcagc agcccgtatc tctatctgga aagctattat
     2881 ctgatctggc aggatcccta tcttttaacc agactgggaa cgtatgaatt gaaagtcctg
     2941 cggtgggcga tgcgccaggg agtcctcaca aaggaacttg cacagcagat caggcatctg
     3001 ctgccggagg tgcgtgagaa gtcgcgggta ctgtacgaga tcttagaggc ggcgtatcag
     3061 gtggagccgg aggaggagat gctttctgcc atctgtgcat atctgatccg cacacagtgt
     3121 tacgaaaccg agtaccaccg gtggtatgag ctcggaatcg agcagaagat aagacttaca
     3181 gggctctatg aggcgtatct gatgtcgctg gatgcgcgtg aggtgggcgg agttccgcgc
     3241 atgattcaga tgtatttcca gtatgacagt acgctttcct atacgcagaa ggcggtcttg
     3301 tttgtcaata tcatagcagc cagaacgaaa cagccggagg tataccagaa atatcagcgc
     3361 acgatggagc agtttgcaat ggcacagatt gaggcggggc acatcaacga tcatctggcg
     3421 gtgatctacg atgagatgct gcccaagggt atactggatg aagaactggc acacagtctg
     3481 gcgccgcttt tgttcacaca cagaatcagc tgcaccaacc gtcagatcgc ccgcgccatc
     3541 atctggcatg aggagatgaa aatgccgcag agcgtgtcgt ttgtgaacgg aaccgcctat
     3601 tttaaggcgt atacgccgca gtatagcatt gtgctggaag atacgaacgg caaccggttc
     3661 tgttcgagtg tggtatatca ggatgaggcg ctgatgtatc cggagcggta tgtgggtcag
     3721 tgtctctcgc ttgcaccggg ggagaacgcc tatctgctct ggcatatgag caggcatatg
     3781 gagccggagg catggagcga taccgagaag acctatgcat tgcagctgct
//
LOCUS       DBMDQJ010000169         3819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8832_length_3819_cov_1.703873, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000169 DBMDQJ010000000
VERSION     DBMDQJ010000169.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8832_length_3819_cov_1.703873"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..500)
                     /gene="rimI"
                     /locus_tag="ACZO9R_04595"
     CDS             complement(63..500)
                     /gene="rimI"
                     /locus_tag="ACZO9R_04595"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR0912380.1"
                     /translation="MSIIRSMTAEDAAKVASLEAEIFSQPWSENAFLDSLCLPDTIFL
                     VAEESGVIQGYIGMYLAADEGEITNVAVNPACRRRGIGEGLLTEMKKRAADHKIARIV
                     LEVRVSNEGAIRLYEKQGFSSVGIRRGFYEFPREDARIMVCAL"
     gene            complement(497..1219)
                     /gene="tsaB"
                     /locus_tag="ACZO9R_04600"
     CDS             complement(497..1219)
                     /gene="tsaB"
                     /locus_tag="ACZO9R_04600"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR0912381.1"
                     /translation="MKILGLDSSGLVASVAIAEDDNLLGEYTVNYKKTHSQTLLPMLD
                     EVARMIELDLSSIDAIAVAGGPGSFTGLRIGSATAKGLGLALEKPILNVPTVDALAYN
                     LVGHRDMVCPLMDARRNQTYTGLYRFDGNDMQVLRGECAVGIDEIAADINSRGEAVVF
                     LGDGVPVFKAYLEEQLTVPHSYAPAHLNKQRAGAVAVLGMQYYREGKTESAAEHRPEY
                     LRLSQAERERKEQEAAKGKENV"
     gene            complement(1216..1665)
                     /gene="tsaE"
                     /locus_tag="ACZO9R_04605"
     CDS             complement(1216..1665)
                     /gene="tsaE"
                     /locus_tag="ACZO9R_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR0912382.1"
                     /translation="MTEKIYETYSPEETHALGKRLGAEAKPGDVYTLVGDLGVGKTVF
                     TQGIAEGLGITEPVSSPTFTIVQVYEEGRMPFYHFDVYRIGDIEEMDEIGYEDYFYGD
                     GLCMIEWANLIEEILPERRSEVTIEKDLEMGFDYRRITIREHQEDRK"
     gene            complement(1685..2182)
                     /locus_tag="ACZO9R_04610"
     CDS             complement(1685..2182)
                     /locus_tag="ACZO9R_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HIR0912383.1"
                     /translation="MPLKFDITLTAEDMYRFNMYQTYSGFHGWFSIAFSILIFVVAGV
                     TSGKIEAAYTALYIVFGIVFLVYPPVSLYLRSKRTLAMSEVLRGTLHYEVDEEGLHVS
                     QKEESALLPWEQIYKMVATKHNVLVYSSRINAYVIPRAQLGESYAALQTQAAAHLPAY
                     RLKMK"
     gene            complement(2268..2831)
                     /locus_tag="ACZO9R_04615"
     CDS             complement(2268..2831)
                     /locus_tag="ACZO9R_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016287973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032217 - riboflavin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0032218 - riboflavin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0912384.1"
                     /translation="MSVTAMLSAVAFILMFLDFSVPFMPAFIKMDLSELPALIGAFSM
                     GPVCGILICLIKNVLHLFITTTGGVGELSNFLLGVAFVLPAGLIYRHKKSRRSALIGS
                     LFGAVIMGVFSVVSNYFLVYPVYYNFMPEEAVLGAYQVIVPSMKSILQCLVCFNMPFT
                     IVKGLFSVVITFLVYKHISPILKGANR"
     regulatory      complement(3081..3243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(3304..>3819)
                     /locus_tag="ACZO9R_04620"
     CDS             complement(3304..>3819)
                     /locus_tag="ACZO9R_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR0912385.1"
                     /translation="GDGKNPLDATGVHPESYDATKRLLERLGYTLSDVKERKVEGISK
                     KIHDYKKLSEELGVGEMTLQDIVKELEKPARDPREDMPKPILRSDVLEIKDLTPGMIL
                     KGTVRNVIDFGAFVDIGVHQDGLVHISQMSDKFIKHPLEVVSVGDIVEVKVLSVDPKK
                     QRIQLTMKLNG"
BASE COUNT          880 a         1146 c          808 g          985 t
ORIGIN      
        1 ttctaatatg aatccgtcag gattcattat atcattgagc cgccgtgcaa tcccacaggg
       61 aattacaggg cacagaccat aattctggca tcttctctcg ggaattcata aaacccccgg
      121 cggataccca ccgaggaaaa tccctgcttc tcgtacaaac ggatcgcccc ctcattcgac
      181 acgcgcactt caaggacaat gcgggcaatc ttgtggtctg cggcacgttt tttcatctct
      241 gtgagcagcc cttccccgat tccacgcctg cggcacgcag gattcacagc cacatttgtg
      301 atctcaccct catcggccgc aagatacatt ccgatgtacc cctgtatgac acccgattcc
      361 tctgccacca gaaagatcgt gtcgggcaga caaagcgaat ccaaaaaagc attctcactc
      421 cacggctgcg aaaaaatctc cgcctccaga gacgccacct tcgccgcatc ttccgctgtc
      481 atgctccgga taatactcat acgttttcct ttccttttgc ggcttcctgc tccttgcgct
      541 ctctttccgc ctgggacagc cggagatatt ccgggcggtg ttccgccgcg ctctccgttt
      601 ttccctctct gtaatactgc atgccaagca cagcaaccgc gccggcccgc tgcttgttca
      661 gatgtgcggg cgcataggaa tgcggcaccg taagctgctc ctccagatat gccttaaaaa
      721 ccggtacacc gtctcccaaa aatacgaccg cctcacctct gctgttgatg tccgccgcaa
      781 tctcatcgat tcccacggca cactctccgc gcagcacctg catgtcgttt ccatcaaaac
      841 ggtacaaacc ggtataggtc tgatttcttc ttgcgtccat aagcgggcat accatatccc
      901 ggtgtcccac cagattgtat gcaagtgcgt ccaccgtcgg cacattgagg atcggcttct
      961 ccagcgcgag tcccagacct tttgccgtcg ccgaaccaat ccgaagtccc gtaaatgagc
     1021 ccggtccgcc tgcaaccgcg attgcatcga tactcgaaag atccagttcg atcatgcgcg
     1081 ccacctcatc cagcatggga agaagggtct gtgaatgtgt ctttttatag tttacggtat
     1141 attctcccag aaggttgtca tcctccgcga ttgcaacgct cgccaccaga ccggaactgt
     1201 ccaatcctaa tattttcatt ttctgtcctc ctgatgctct cttatggtaa ttctgcgata
     1261 atcaaatccc atttccagat ctttttctat agtaacttcg ctgcgccgct ccggcagaat
     1321 ctcttcgatc agattcgccc actcgatcat gcacagacca tccccgtaga aataatcctc
     1381 gtagccgatc tcatccatct cctcgatgtc cccgatgcga taaacgtcga aatgataaaa
     1441 cggcatcctg ccctcctcat atacctgcac gattgtaaag gtcggactgg agaccggctc
     1501 cgtgatgcca agtccttccg caattccctg tgtgaagact gtctttccga cgcccagatc
     1561 tcccaccagc gtatagacgt cgccgggctt tgcctccgcg cccaggcgtt ttccaagcgc
     1621 gtgcgtctct tccggcgaat atgtttcata gatcttttct gtcatatttt aaaatcccca
     1681 ttctttactt catcttcaaa cggtacgccg gcagatgtgc cgcagcctgt gtctgcagtg
     1741 ccgcgtagct ttcccccagc tgtgctctcg gaatgacata ggcattgatc cggctgctgt
     1801 acaccagaac gttgtgcttc gtcgcgacca ttttatagat ctgctcccac ggaagcagcg
     1861 cactctcttc cttctgcgat acgtgcagcc cctcctcgtc cacctcatag tgcagcgttc
     1921 cccgcagcac ctcggacatt gccagtgtgc gcttcgacct taggtacagg ctcaccggag
     1981 gatagaccag aaatacgatg ccaaatacaa tatataatgc ggtgtatgcc gcctcgatct
     2041 tcccgctggt cactcctgcc accacaaaaa tcaggatgga aaacgcaatg gaaaaccacc
     2101 cgtgaaatcc ggagtaggtc tggtacatat taaaccggta catatcttct gctgtcaatg
     2161 taatatcaaa ctttagcggc ataacatttc ctcctgtcta tatctaagcc agtatagcat
     2221 ttttcattcc aaaagaaaag gggctgtcgt ctaagacagc cccttttcta gcggttcgct
     2281 cctttcagaa tcggcgaaat gtgtttgtat accaagaatg tgattaccac ggaaaacagt
     2341 cccttcacga tggtaaacgg catattgaag cacaccaggc actgcagaat gctcttcatg
     2401 gatggtacga tcacctgata agcgccgagc acagcttctt ccggcataaa gttgtagtat
     2461 accggatata ccaggaagta gttggatacc acggaaaata cacccatgat caccgcaccg
     2521 aacagggatc caatcaatgc gcttctgcgg ctctttttgt ggcggtaaat caatcctgcc
     2581 ggaagtacga atgctacgcc cagaaggaag ttggaaagct caccgacgcc gccggttgtt
     2641 gtaataaaca gatgaagcac attcttgatc agacagatca gaatgccgca taccggtccc
     2701 atggaaaatg caccgatcag tgccgggagc tctgagagat ccatcttgat gaaagccggc
     2761 ataaacggaa cggaaaaatc caggaacatt aaaataaatg ctactgcgga aagcattgcc
     2821 gtgacgctca tgtagcgaac gctcgaaata ctgtttgttg ttgtcctacc tgtgattgta
     2881 ctgtttgtgt ccataatgac tcgtctcctt ttctctgcag gaattcgagg tattctcgcg
     2941 tgtcagccgt ctttcaacgg attgcattcc ggcagcggaa cagcgcttat tcccgccaca
     3001 caaaaagccc cggatgatct ctacatccgg ggcgttaaat tgcattctta agaccctgct
     3061 gccgcaggtc ctcctaacat gcttctcttc tctcatccag actatactgt cggtcccgga
     3121 ttcacaccgg ctcaactgca actgcagctc acggactgat ctggcgtctg ccagaatcac
     3181 cgtcggtcgg gaatctatgc ttccatctct gtgaactcat atcaccctgc cccgaagaat
     3241 tcctaatatt tattttgctt ttatattgta tgcccatatg ggcatactgt caaccctttt
     3301 tctctatccg ttcagcttca tcgtcagctg gatgcgctgc tttttcggat caacgcttaa
     3361 tactttcact tccacaatat ccccgacgct gacaacttcg agcgggtgct tgataaactt
     3421 gtcgctcatc tgtgaaatat gcaccagtcc gtcctgatga acaccaatat cgacaaacgc
     3481 gccaaaatca atgacgttgc gcacggttcc tttcagaatc atccccggcg tcaggtcttt
     3541 gatctcaagc acatcgctgc gcagaatcgg ctttggcatg tcctctctcg ggtctctcgc
     3601 cggcttctcc agctccttga caatgtcctg cagcgtcatc tctcccacac ccagttcttc
     3661 cgaaagtttt ttgtagtcat ggatcttttt ggaaatgccc tctaccttcc gctcttttac
     3721 gtcagataac gtataaccca gccgctccag aagcctcttc gtggcatcat agctttccgg
     3781 gtgcacaccg gtcgcatcga gcggattctt tccgtcgcc
//
LOCUS       DBMDQJ010000170         3819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8834_length_3819_cov_1.201990, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000170 DBMDQJ010000000
VERSION     DBMDQJ010000170.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8834_length_3819_cov_1.201990"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="ACZO9R_04625"
     CDS             complement(<1..401)
                     /locus_tag="ACZO9R_04625"
                     /inference="COORDINATES: protein motif:HMM:NF024398.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3880 domain-containing protein"
                     /protein_id="HIR0912386.1"
                     /translation="MYDCPHVTLYSDSLRNSCSYIFLFDRNMQQDAVMHGALHAYHLP
                     LAINADRLASHLSLSGSRDTFFPTTYRHEVSFVGSLYEKTTFEHLKNVPPHLKGYLDG
                     IIAAQKQVWGADVISAALSPDHVNEIYQALP"
     assembly_gap    477..513
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            782..1411
                     /locus_tag="ACZO9R_04630"
     CDS             782..1411
                     /locus_tag="ACZO9R_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912387.1"
                     /translation="MQNRETSEEFAREQENANKGWEICYGASAKAYLEWMDQMLEQGT
                     AEAHEKLLAFFEDENKTAPYGNRSNAMIEMIVIMDIYKAEVQMAEKNTILDRKIQGKP
                     MRRSELTAYMREFRFLMWRLEFTDEADAGVNFIEFLKANQVSPVFLCKAVNTMAADEF
                     SMLCVIMELTLDAKMFRHAYWLLLKMQRLAPGEESIRKMLEELKAYVTG"
     gene            1408..2073
                     /locus_tag="ACZO9R_04635"
     CDS             1408..2073
                     /locus_tag="ACZO9R_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0912388.1"
                     /translation="MKVTVDERKFAFIMCTNNRQYCLEALHYIKSLAVPDGYTVETCI
                     IEDAKSMTEGYQRAMLSSGAKYKIYLHQDVMIVEKNFLQHLLDIFADRKIGMIGMIGS
                     PRMPENSVMWYGERIGCIYSSSAYHMQMYTAGAVEQPYQQVEAVDGLLMATQYDIPWR
                     EDLFQKWDFYDISQAFEFRRNGYQVVVPAMEKPWCIHDCGASDFKNYFEERRKFQKEY
                     RGR"
     gene            2075..3175
                     /locus_tag="ACZO9R_04640"
     CDS             2075..3175
                     /locus_tag="ACZO9R_04640"
                     /inference="COORDINATES: protein motif:HMM:NF025089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0912389.1"
                     /translation="MNDRQIAFIMCVNDDRQADESEFYIRCLEVPEQYEVDVIRVREA
                     ESMTAGYNAAMENSDARYKVYLHQDTRIIAPGFIRDMINVFKNDDTIGMLGCVGCRKL
                     PANGQAVGHWDAGMVYHNCIPGMLTLEQDKEQQPVEVEALDGLLLATSRDVRWREDLF
                     DGWDFYDVSQCLEMRRMGWRVVVPCQETPWCYHDNFYSRMLNYQKYCNKMITEYQDIQ
                     SFQAMDYSESRKELEQIKEVARKELYELVDKGEKAQFLELFQEQENRGWLHLKDLEVL
                     ADIAAAERDAGQECFWMPGEPGLELHEKLLKLHRILKRIEYHKDVFDMNIYRELEAYS
                     IPAVKTVLCAYTEKPQAVWDRIQEEMWKRQEH"
     gene            3154..3588
                     /locus_tag="ACZO9R_04645"
     CDS             3154..3588
                     /locus_tag="ACZO9R_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912390.1"
                     /translation="METTGALTGESFDKQKTLLEIYAAERRAGIRHAAPDIEVPDIDE
                     FWKPIRDLRFLIWRVIAAGVPETGEELCRKIQKENISPVALLFVIRTATEKPEDILAP
                     LADLFIDHQMLAHAYYLLKELQKQMSNVADIQELVTELERYL"
     gene            3623..>3819
                     /locus_tag="ACZO9R_04650"
     CDS             3623..>3819
                     /locus_tag="ACZO9R_04650"
                     /inference="COORDINATES: protein motif:HMM:NF025089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912391.1"
                     /translation="MCVNDDAYAKEAVWYIRHLKLPDGYHIEWQCVKGAMSMAAGYNE
                     AMKKSNARYKVYLHQDVMILET"
BASE COUNT         1117 a          618 c         1118 g          929 t
ORIGIN      
        1 aagggaagtg cttgataaat ttcgtttacg tggtcgggtg aaagtgctgc cgagatcaca
       61 tctgcccccc agacctgttt ctgagcggca atgatgccgt caagatatcc tttcaggtgt
      121 ggcggaacat tcttcaggtg ctcaaaagtc gttttctcat acagagaacc aacaaaggag
      181 acctcatggc gataagttgt cggaaagaac gtatcgcggg aacctgatag ggaaaggtgg
      241 cttgccagac ggtctgcgtt tatggcgagt ggcaggtgat aagcgtgaag cgcgccgtgc
      301 atgactgcgt cctgctgcat attccggtca aacaggaaaa tgtagctgca ggaattgcgt
      361 aaactgtcgg aatacaatgt gacatgtgga cagtcataca cccagcagat atagggaatc
      421 tgtatggatt ctgcaatatt tgagaccggt ggtatgaagt taaaggtaaa gatacannnn
      481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngggaagc agcgcatctg aaaaatagaa
      541 cgataattca tgcagctggg cacagacgtt gtgtccaagc tgttgtaggg tttcgatcat
      601 gtctgaggca gaattttctg tccagcttaa gtatagtata ttcatagata atggaatcct
      661 ttcggaaaat atgtatgcct ggctgccccg ggttgtctgt gggctgagga tacagtataa
      721 tagatcaaag agattataag ataagaacaa ttatttggca ataaaattta caggggacaa
      781 tatgcaaaac agagaaacat cagaggagtt tgcgcgggag caggagaatg caaataaagg
      841 ctgggaaata tgctatggcg cgtcggcaaa ggcgtatctg gaatggatgg atcagatgct
      901 ggagcaggga acggcggagg cacatgagaa gctgcttgca ttttttgagg atgaaaataa
      961 gacggcgcca tatggaaaca gaagcaatgc aatgatcgag atgattgtca ttatggatat
     1021 ctataaggcg gaagtgcaaa tggcggaaaa gaataccatc cttgacagga agatacaggg
     1081 aaaaccaatg cgaagaagtg agctgacggc atatatgcgg gagttccggt tccttatgtg
     1141 gagactggaa tttacggatg aggcggatgc aggtgtgaac tttattgaat ttttaaaagc
     1201 aaatcaggta tcgcctgttt ttctctgtaa agcggtgaat acaatggcgg ctgatgaatt
     1261 ttccatgcta tgtgtcatta tggaattaac cctggatgcc aaaatgttca gacatgccta
     1321 ctggctgctt ctgaagatgc agagactggc gccgggggag gaaagtatcc ggaagatgtt
     1381 agaggaattg aaagcatatg tgacggggtg aaagtaacgg tggatgagag aaagtttgcg
     1441 tttatcatgt gtacaaacaa caggcaatac tgcttggagg cactgcatta tataaagtcg
     1501 ctggcagtgc cggacggcta taccgtggaa acatgtatca ttgaagatgc taaaagtatg
     1561 acggagggat accagcgggc aatgctgtct tccggtgcaa agtataagat atatctgcat
     1621 caggatgtca tgattgtcga aaaaaatttt ttgcagcatt tattggatat ttttgctgac
     1681 cggaaaatcg gtatgattgg catgatcgga tctccccgga tgccggaaaa cagcgttatg
     1741 tggtatgggg aacggatcgg ttgtatttac agcagcagtg cttaccatat gcagatgtat
     1801 acggcgggtg ctgtggaaca gccgtatcag caggtagaag cggtagatgg gcttttgatg
     1861 gcgacacagt acgatattcc gtggagagaa gatttgttcc agaaatggga tttttatgat
     1921 atttcacagg cgtttgagtt tcgcagaaac gggtaccagg tcgtggtgcc ggcgatggaa
     1981 aaaccatggt gtattcacga ctgtggagca tctgatttta agaactattt tgaggaacgg
     2041 agaaagttcc agaaggaata taggggcagg tagaatgaac gacagacaga ttgcgtttat
     2101 tatgtgtgtg aatgatgaca gacaggcgga cgaatcagaa ttttatatac gttgtctcga
     2161 ggtgccggaa cagtatgagg tggatgtgat ccgggtgcgc gaggcggaga gcatgacggc
     2221 gggatataat gcggcaatgg agaattcaga cgccagatat aaagtatatc tgcatcagga
     2281 tacgcggatt attgcgccgg gatttatcag agatatgata aacgtgttta aaaacgacga
     2341 tacaataggc atgcttggct gcgtgggctg tagaaaactg ccggcaaatg gtcaggcggt
     2401 aggacattgg gatgccggta tggtgtacca taactgtatt ccgggaatgc tcacactgga
     2461 gcaggataaa gagcagcagc cagtggaagt ggaagcgttg gatggattgc tgcttgcgac
     2521 atccagggat gtccgttggc gggaagatct gtttgatggt tgggatttct atgatgtgtc
     2581 gcagtgtctg gagatgaggc ggatgggctg gcgggttgta gtgccttgtc aggagacgcc
     2641 ctggtgttac catgataatt tttacagcag aatgttaaac tatcagaaat attgtaataa
     2701 aatgatcacg gaataccagg acattcagtc cttccaggcg atggattact ccgagagcag
     2761 aaaagaattg gagcagatca aggaggttgc cagaaaggaa ctgtacgagc ttgtggacaa
     2821 aggagaaaag gcgcagtttc tggaattgtt tcaggagcag gagaaccggg gctggcttca
     2881 tctgaaagat ctggaggtgt tggcggatat tgcggcggca gagcgggacg cggggcagga
     2941 gtgtttctgg atgccgggcg agccgggact ggaacttcat gaaaagctgc tgaagcttca
     3001 tcggatatta aagaggatcg aataccataa ggatgtattt gatatgaata tttacaggga
     3061 actggaagcg tacagtatac ccgctgttaa gacggtattg tgtgcataca cggagaagcc
     3121 acaggcggta tgggaccgga ttcaggagga aatgtggaaa cgacaggagc attaacagga
     3181 gaaagttttg ataaacagaa aactttattg gaaatttatg cggcggaaag gcgggcgggt
     3241 atccgtcatg cagcgccgga tatagaggta ccggatatag acgaattctg gaaaccgatc
     3301 agagatttgc ggtttttgat ctggagagta atcgcagcgg gagtcccaga aacgggggag
     3361 gagctttgcc ggaagattca gaaggaaaat atatccccgg ttgcattgct gtttgtgatt
     3421 cgcactgcaa cggaaaaacc ggaggatatt ctggcaccat tggcagatct ctttattgat
     3481 catcaaatgt tggcacatgc atattatctt ttaaaagaat tgcagaaaca aatgtcgaat
     3541 gtggcggata tacaagaact ggtaacggaa ttagaaagat atttataggg ggattatgga
     3601 cgagcataat atatgtttta ttatgtgtgt aaatgacgat gcgtatgcaa aggaagcagt
     3661 ctggtatatc agacatttga aattacctga cggttatcat atagaatggc agtgtgtaaa
     3721 aggtgctatg agtatggcgg caggatacaa tgaggcaatg aaaaagtcga acgcacggta
     3781 taaggtttat ttacatcagg acgtgatgat tctggaaac
//
LOCUS       DBMDQJ010000171         3795 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8903_length_3795_cov_1.627504, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000171 DBMDQJ010000000
VERSION     DBMDQJ010000171.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8903_length_3795_cov_1.627504"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="ACZO9R_04655"
     CDS             complement(<1..122)
                     /locus_tag="ACZO9R_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008751000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HIR0912392.1"
                     /translation="MAEKTNANIGFEKQLWDAACVLWGHIPAAEYRKVIIGLIFL"
     gene            538..1332
                     /locus_tag="ACZO9R_04660"
     CDS             538..1332
                     /locus_tag="ACZO9R_04660"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR0912393.1"
                     /translation="MIKLIATDVDGTLVKDGTMQIDTEYMTVIKELAQKGIIFAVCSG
                     RQFISERKLFAPIKDQLLYITDGGTVVRTPQEILMVHTMPRDVWSGMCRMVQEQMPHC
                     DCFVATPDYCLAEDAGSRMFHWLRDSYGYDIREAERLADIPEQDIIKFTVYHKSACEE
                     MCAPLFTPAWKDKAQLAAAGKEWMDCNPLGANKGTAIEFVQKHFGISPEETCTFGDNL
                     NDIEMLQNAGRSYAVANAREEVIAAAKDTCAPYWENGVLQILKTFL"
     gene            complement(1946..3526)
                     /locus_tag="ACZO9R_04665"
     CDS             complement(1946..3526)
                     /locus_tag="ACZO9R_04665"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006428043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR0912394.1"
                     /translation="MVTWNNLDTLASFKELSKMERVNLVEAMSGENGADRVKNYSVPM
                     AGGFSYNYAAKQVDDKVLAALVKLAEEAQLAEKFEALYNGEVINTGEKRLVLHHMTRG
                     QLGDAVTADGVDKRSFYVEQQERIADFANKVHSGEITNAAGEKFTTVVQIGIGGSDLG
                     PRAMYLALENWAKKNGKFKMEAKFISNVDPDDAAAVLNSTDVAHSIFVLVSKSGTTLE
                     TLTNESFVKDALKKAGLDPSKHMIAVTSETSPLAKSDDYLAAFFMDDYIGGRFSSTSA
                     VGGAVLSLAFGPEVFAQFLEGAAAEDQLSANKDVLKNPEMLDALIGVYERNVLGYPAT
                     AVLPYSQALSRFPAHLQQLDMESNGKSVNRFGEPVDYPTGPVIFGEPGTNGQHSFYQL
                     LHQGTDIVPLQFVGFKNSQIGTDVVIQDSTSQQKLCANVAAQIVAFACGKSDENRNKN
                     FEGGRPSSIIIGEQVNPQSLGALLAHFENKIMFQGFLWNVNSFDQEGVQLGKVLAKRV
                     LAHETDGALKVFSDLLNI"
BASE COUNT          939 a         1035 c          887 g          934 t
ORIGIN      
        1 agaaaaatga gtccgatgat aacctttcta tattccgctg ccgggatatg tccccaaaga
       61 acgcaggctg catcccacaa ctgtttttca aagccaatgt tggcatttgt tttctctgcc
      121 attatagtat cctccacatt gagtatttat ctttcaaaat gttctgtctc catgaattta
      181 tgatttatta ctgccattct accattccct aaatacttat tcaacaatac atctgtaatc
      241 aaggataaat ttcgacgata aaatccgagt acatacggaa attggttggt cgccattttg
      301 acctgcaaat ataaaaaaca tcataggcaa tagcaaggga ataagacact cttcaaaatc
      361 cctatcatag ttagcactca tttcttcttg cctatttttt accgaaaatg tcctgtaaac
      421 acaatgatgg caaccgttgg tatttgcaaa atatgcttcc cgacgttata atacagctgt
      481 tgtatccaga aacatttaca gaacagggca cacatcagcc ccggaacgga gaatcccatg
      541 ataaaactga ttgccaccga tgtcgacggg acactggtaa aagacggaac gatgcagatc
      601 gataccgaat acatgactgt cattaaggaa cttgcccaga aaggaatcat ttttgccgtc
      661 tgctccggca gacagttcat cagcgagcgg aagctgtttg cgccgatcaa agatcagctg
      721 ctctacatca cggacggcgg cacggtcgtg cgcaccccac aggagattct gatggttcac
      781 acgatgccgc gcgacgtctg gagcggcatg tgccgcatgg tacaggaaca gatgccgcac
      841 tgcgactgtt ttgtcgccac accggactac tgtctcgccg aggacgccgg aagccgcatg
      901 ttccactggc tgcgcgattc ttacggctac gatatccggg aggcagagcg cctagccgac
      961 atcccggagc aggacatcat caagtttacc gtctaccaca aatccgcctg cgaggaaatg
     1021 tgtgcgccgc tcttcacccc ggcatggaaa gacaaggcac agcttgcagc cgccggcaaa
     1081 gagtggatgg actgcaatcc gctcggtgcg aacaagggaa ccgcgatcga gttcgtacag
     1141 aagcatttcg gcatttcacc ggaagaaacc tgcacgttcg gggacaactt aaacgacatt
     1201 gagatgctgc agaacgccgg caggagctac gccgtcgcca acgccagaga ggaagtcatc
     1261 gccgcggcaa aagatacctg tgcgccctac tgggagaatg gagtcctcca gatcttaaaa
     1321 acatttttat aacgaaaaag aatcgtgtcc atccagcggc agtgccccgg cgtacacggt
     1381 tcttttttat ttctgcagaa gtccgccgcc atattggatg cggttcctgt caatgaattc
     1441 tttggaacaa aatttctatt tttcttcatg catgccccgg tcatccattt tcctgtctct
     1501 tttggcgttc gactgcgcgc cgcgggtgtc cgcggacaac ggacaaagcg ccggctttct
     1561 agtctcgcta ggcagaccgc ttcacaaagt tccgatcaga ccgcgcaatc cgaaactcgg
     1621 tggctgcccg cattcaaaac acggcggcag acacctcaga cagtcgtttg cgcggtccga
     1681 ttttgctcat cggtctgcca agcgagacac aaaagccgtc gaaaatgtcc gcctgtccgc
     1741 gaacacccgt ggcgcactgt cgaacctcaa aaagacagga atcatcactg atggccgggg
     1801 catgcgtgtt agaaaaatga aattttgcga cagaatacgt tggcaggaac tgcacccaat
     1861 atgccaaacc ttcccaacgc acaaaaaaag aaccgtgtac gccgcgcatc tgccgcggga
     1921 cgtgacacag ttctttttcg ttatattaaa tgtttaacaa atcgctgaat accttcaggg
     1981 caccgtcggt ctcatgtgca agcacgcgct ttgccagaac ctttccgagc tgtacgcctt
     2041 cctgatcgaa gctgttcaca ttccacagga agccctggaa cataatcttg ttctcaaagt
     2101 gtgccagaag tgctccgaga gactgcgggt tcacctgctc accaatgatg atgctggatg
     2161 gacgtccacc ctcgaaattc ttgttgcggt tctcgtcgga tttgccgcat gcgaatgcca
     2221 cgatctgagc tgcgacattt gcgcagagct tctgctggct tgtgctgtcc tggatcacaa
     2281 catctgtgcc gatctggctg ttcttaaagc cgacaaactg aagcggtacg atgtctgtgc
     2341 cctgatgcag cagctggtag aacgaatgct gtccgttcgt gcctggctca ccgaagatca
     2401 ccggtcctgt cgggtagtct accggctcac cgaaacggtt caccgactta ccgttggact
     2461 ccatgtcaag ctgctgtaaa tgtgccggga agcggctgag tgcctgggag tacggaagca
     2521 ctgccgtagc cggatagccc agtacgttgc gctcgtaaac gccgatcagt gcatcgagca
     2581 tctccggatt cttcaatacg tctttgtttg cggaaagctg atcctctgca gcagctccct
     2641 cgaggaactg tgcgaacact tccggtccga acgccaggga taatacggca ccgccaactg
     2701 cggaagtgga ggagaaacgt cctccgatat agtcatccat aaagaatgcc gcaaggtaat
     2761 catcactctt tgccagcggg gatgtctcgc tcgttacagc aatcatatgc ttggaaggat
     2821 ccagtcctgc tttctttaac gcatctttta cgaaggactc gttcgtcagg gtctccagtg
     2881 tcgtaccgga cttggatacc agtacgaaaa tggaatgtgc cacatcggtg gaattcagca
     2941 ctgctgcagc atcatccggg tctacgttgc tgatgaactt cgcttccatc ttgaacttgc
     3001 cgtttttctt cgcccagttc tcaagtgcca gatacatcgc acgcggaccg aggtcactac
     3061 caccgatacc gatctgaaca actgttgtga acttttctcc tgccgcattg gtgatctcgc
     3121 cggaatgtac cttgttggca aaatcagcga ttctctcctg ctgctctaca tagaagctgc
     3181 gcttatccac accgtctgcc gtcacagcat caccgagctg tcctcgtgtc atgtgatgaa
     3241 gcaccagacg cttctcaccg gtgttgatca cctcaccatt gtaaagtgcc tcaaacttct
     3301 ccgcaagctg tgcttcctca gccagcttta caagtgctgc aagcacctta tcatccacct
     3361 gcttggctgc atagttgtaa gaaaatccgc cagccatcgg cacactgtaa ttcttcacac
     3421 ggtcagcacc gttctctccg gacattgcct cgacaagatt cacacgttcc attttggaaa
     3481 gctccttgaa agaagcaagg gtatccaagt tgttccaggt aaccataata gattcctcct
     3541 atctattctt gcacatattc atattttatt gatcgtagtg attatactat taaatgatct
     3601 caagttcaag taaaacggcg gagagccaat cgctctccgc cgtataatga tttattttgc
     3661 ggcagccgga ttcagtttct tcgtgcggat atcctcctgc aacatggcga ggaagtcttc
     3721 catggatacc gtcgtagact gctgctctcc gtgaagtctg tagcttaccg tccggtctgc
     3781 cacttcacgg agagc
//
LOCUS       DBMDQJ010000172         3794 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8908_length_3794_cov_1.479014, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000172 DBMDQJ010000000
VERSION     DBMDQJ010000172.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8908_length_3794_cov_1.479014"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            47..>355
                     /locus_tag="ACZO9R_04670"
     CDS             47..>355
                     /locus_tag="ACZO9R_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0912395.1"
                     /translation="MFTDPDVRTALINSLKMMVLTVIFEVGIAFVLAVMVSQIKKLQQ
                     LFRTVYFFPVVISATAIGLLFKLFYNYNGGMLNQILESIGAKPVNWLSPSLAFIMVCI
                     P"
     assembly_gap    356..365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            499..969
                     /locus_tag="ACZO9R_04675"
     CDS             499..969
                     /locus_tag="ACZO9R_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0912396.1"
                     /translation="MLRRFFLVCIPTLWSYIGFYFVILLTGLSDIPDDIYEAASIDGC
                     SRFKQVFYITLPMMKPVFFTSVTLAVTGALKVFDLPWVMVPNGAPQGLTHFLGTYMYQ
                     QTFGLNNYDYGSTLAVLIVVLGIVVSKAVGFLTADADDKEAKKRARAEKKKGVS"
     gene            970..1836
                     /locus_tag="ACZO9R_04680"
     CDS             970..1836
                     /locus_tag="ACZO9R_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0912397.1"
                     /translation="MDAAVCAKAVRKKHRRIGAGTVVIYLVLIFWAVLTIFPFVWVVL
                     NSFKPSAEVLRSSFSLPETFTWMNYQTAFERLNVLRAYRYSFVISGTVTVGVMVFASL
                     MAFGLTRYHFKGKELIRSLIVASLMFPVFSTIIPVFKMMVEWGLLNKSIAVILPQIAG
                     NLSFATIVMTGFMQSIPLEMEEAAYMEGADVFKVFGKIIVPLCRPSLATVAIFSFLWS
                     YNDLFVQKIMMRDPTRYPISTLLEQISSQYGTDFGLMAASVTIIVIPVMIVYVLLQKN
                     IIKGLTAGAVKG"
     gene            1882..2661
                     /locus_tag="ACZO9R_04685"
     CDS             1882..2661
                     /locus_tag="ACZO9R_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0912398.1"
                     /translation="MYKVMIVDDEPIIVEGLSRSIAWEKWNCKVAATAHDGLEGKKII
                     EEIHPDIVFMDICMPEMDGLAMIAAIHSQFPDLEVCVLTGYRDFEYAKEAIRLGVTRF
                     LLKPSNMDELEEAIGKMCGNLNRKGITGDEPKRDPQDGEAAEEHKESASSSFIVKNAL
                     TYIEENYTKKLTLCEVAEKTYVSQWHLSKLLNRHTGQSFSDILNHVRIEHAKELLKNP
                     SLRIGDISEQVGFLDLAHFSRVFKKQEGVSANEYRNQVLGK"
     gene            complement(2674..3168)
                     /locus_tag="ACZO9R_04690"
     CDS             complement(2674..3168)
                     /locus_tag="ACZO9R_04690"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR0912399.1"
                     /translation="MTRESYEKITGILRGHPRRIRLVCLFNHTLTGFIFVLYPAFLLL
                     LFLEGSAFFLRALLVPAVSFAAVTLFRRIFNAPRPYEKFGLPPAIEKDTAGKSFPSRH
                     VFSVFIIAATIFYVHPSAGILLGLLGVVLAVLRVIGGVHEPRDVIAGALAGILCGVLG
                     YYVL"
     gene            complement(3165..3617)
                     /locus_tag="ACZO9R_04695"
     CDS             complement(3165..3617)
                     /locus_tag="ACZO9R_04695"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR0912400.1"
                     /translation="MVYAIIAGGVFLSDLILKRYMDKKYARKVRHPRLGGRIVLEKYY
                     NKGAALNFMVKRPKLLRIIHTVILVVVGIFSYFLMRLSGHALEKTGVALLLGGGLNNL
                     YDRYTKGHVVDYFHLNFGPKWLRAIVFNISDFCIFIGALLVVAGSEVS"
BASE COUNT          985 a          833 c         1053 g          913 t
ORIGIN      
        1 gccgggaact gaatggaagt ttattggatt tgaaaattac aagaaattgt ttacagaccc
       61 ggatgtgagg acggcgctca tcaattccct gaagatgatg gtgctcacgg tgatttttga
      121 ggtcggaatc gcatttgtgc ttgcggtaat ggtttcacag ataaaaaagc ttcagcagtt
      181 attccgtacg gtgtactttt ttccggtagt gatttctgca acggcgattg gtctgttgtt
      241 caaattattc tacaactata acggtggtat gctgaatcag attttagaga gcattggagc
      301 aaagccggtc aactggcttt ctcccagcct ggcatttatc atggtctgta ttccgnnnnn
      361 nnnnngaagc cgcccacaaa ggcacacagc gcattgaaca gtgtcaccga cagtgccaga
      421 gacaacacca ccagtaccgt ctttttcttg tttcgtccca gattggcaaa ggccatctga
      481 tggagctttg ctcctcggat gctgcgccgt tttttcttgg tctgtattcc gacgctgtgg
      541 agttacatcg gtttttactt tgtcattctc ctcacggggt taagcgatat tcctgacgac
      601 atatatgaag cggcgtccat cgacggatgc agccgtttca agcaggtatt ttatattaca
      661 ctgccgatga tgaaaccggt attttttaca tcggtcacac tggcggtaac gggagcgtta
      721 aaagtattcg atcttccgtg ggtgatggtg ccaaacggcg caccgcaggg gctgacacat
      781 tttctcggaa cgtatatgta ccagcagacc ttcggactca acaattatga ttacggttcc
      841 acgctggcgg ttctgatcgt ggtgctcggc attgtggtat cgaaagcggt cggttttctt
      901 acggcggatg cggacgataa agaagcgaaa aagcgcgcaa gagcagagaa aaagaagggg
      961 gtgtcttaaa tggatgcagc agtatgtgca aaggcagtca gaaaaaaaca caggagaatc
     1021 ggagccggta cggtcgtcat ttatctcgta ctgatctttt gggcggtgct caccattttc
     1081 ccgtttgtgt gggtggtgct gaattccttt aaaccgtccg cggaggtgct ccgctcctcc
     1141 ttctcacttc cggagacgtt cacctggatg aattatcaga cggcatttga gcggttaaac
     1201 gtcttaagag cctataggta cagcttcgtg atctcaggca cggtaacagt aggcgttatg
     1261 gtattcgcgt cgctgatggc attcggactt accagatatc atttcaaggg caaggagctg
     1321 atccgcagcc tcatcgtggc aagtctgatg tttccggtat tttccacgat cattccggtg
     1381 tttaagatga tggtggaatg ggggcttctg aacaaaagca ttgcggttat cctgccgcag
     1441 attgccggaa acttaagctt tgcgacgatc gttatgaccg gtttcatgca gagcatcccg
     1501 ctggagatgg aggaagctgc ctacatggaa ggcgcagatg tatttaaggt atttggaaaa
     1561 attattgttc cgctttgcag accgtcactt gcaacggttg ccatctttag tttcctctgg
     1621 tcttataacg atctgtttgt acagaaaatc atgatgcgtg acccgaccag gtatccgatc
     1681 tccacactgt tagaacagat cagttcccag tatggaaccg atttcggatt gatggcggcg
     1741 tcggttacga tcattgtcat cccggtcatg attgtgtatg tgctgttaca gaaaaatatc
     1801 atcaaaggat tgacagcagg tgccgtgaaa ggctaaactt cttggaaaga gagcggaatt
     1861 tgctcaagtg gaggattggc tatgtacaag gtgatgattg tggatgacga accgatcatc
     1921 gtggaagggt tatcgaggag catcgcgtgg gaaaagtgga attgtaaggt cgcggcaacg
     1981 gcgcacgacg ggctggaagg gaaaaagatt atcgaggaga ttcacccgga tattgtgttt
     2041 atggatatct gcatgccgga gatggatgga ctggcgatga tcgctgcgat ccactcgcag
     2101 ttcccggatc tcgaggtctg tgttctgacg ggctaccgtg atttcgagta cgcgaaggag
     2161 gcaatccgtc tcggggtgac gcgctttctg ctcaagccgt ccaacatgga cgaactggaa
     2221 gaagccatcg gaaagatgtg cgggaactta aatcggaaag gaattaccgg ggatgagccg
     2281 aagcgtgacc cgcaggatgg ggaagcggcg gaagagcaca aggagagtgc atcgagcagc
     2341 tttatcgtga agaatgcact gacttacatt gaggagaatt atacgaagaa gctgacgctc
     2401 tgcgaggtgg cagagaagac gtatgtgagc cagtggcact taagcaagct tttaaaccgc
     2461 cacacggggc agagcttttc agacattttg aatcacgttc gtatcgagca tgcgaaagaa
     2521 ctgctaaaaa atccgtcgct gcgcatcgga gatatctccg agcaggtggg atttttagat
     2581 ctggcacatt tctcgagggt atttaagaag caggagggag tctctgccaa tgaataccgg
     2641 aatcaggtac tgggaaagta aggtgccgga cggctagagt acatagtatc cgagcacgcc
     2701 gcacagaatc cctgcaagag cgcctgcgat gacgtcgcgc ggctcatgaa cgccgccgat
     2761 cacgcgcagg acggcaagga caacccctaa gagtccgagc agaatgccgg cggaagggtg
     2821 gacatagaaa atggtggcgg caatgataaa tacggaaaag acatgacggc tgggaaaaga
     2881 tttcccggcc gtatcttttt ctatggcagg cggaaggccg aacttctcat aaggtcttgg
     2941 ggcgttaaag atccggcgga agagcgtgac tgcggcaaag gagacggcgg gaacgagaag
     3001 cgcccgcagg aaaaaagcgc tgccctcaag aaaaagcagc agcagaaacg ccgggtacag
     3061 cacaaaaata aagccggtca aggtgtggtt gaacaggcat accagccgga tgcgccgcgg
     3121 atgtccacgt aagatgccgg tgattttttc ataggattct ctggtcatga gacctccgat
     3181 ccggcaacga cgagcagtgc cccgatgaaa atgcagaagt cagagatgtt gaagacgatc
     3241 gcgcgcagcc atttcggacc gaaattcaga tgaaaatagt cgacgacatg cccctttgtg
     3301 tagcggtcgt ataggttgtt tagaccgccg ccgagcagga gcgcgacgcc cgttttctcc
     3361 agcgcgtgtc cgctaagacg catcagaaaa taggaaaaaa tcccgacaac aacaagaatg
     3421 acggtgtgga tgatgcgcag aagctttggc ctttttacca tgaaattcaa agccgcgcct
     3481 ttgttataat atttttccag gacaatcctg cccccaagcc gcggatggcg caccttgcgg
     3541 gcatattttt tgtccatgta acgctttaag atgagatccg acaggaatac cccgccggca
     3601 ataatcgcat aaaccatgta aaaatctcct gacaggtatt gatgtatgta tcatatcatc
     3661 ttccatggaa aatgaaaagt gatttgcgct tgcctttttt tatccctgtt tgtgcagttg
     3721 cccgcaaacc cttgaaaggg cgttgcctgg ctgataaagt caagacagag gcagaaaaat
     3781 ctgcagtaaa taac
//
LOCUS       DBMDQJ010000173         3783 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_8933_length_3783_cov_1.491309, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000173 DBMDQJ010000000
VERSION     DBMDQJ010000173.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8933_length_3783_cov_1.491309"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="ACZO9R_04700"
     CDS             complement(<1..882)
                     /locus_tag="ACZO9R_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912401.1"
                     /translation="MNYFMSLDPLALFRSMPGAFAQGMVWGLMALGVYITYRLLDFAD
                     LTVDGSLATGGAVAVMLIRGGMSPWAALIFAFLAGMVAGFITGILHTALGIPGILASI
                     LTQISLYSINLNIMGCSNQAVSVDKYPLVVSLRYLSDSNSTRVQIFATCILFCFAIVA
                     ILYWYFGTEQGHAIRATGCNQQMARAQGINTNLHTVIALMISNGLVGLSGGLYAQYQG
                     AADVNMGRGAIVIGLAAVIIGEVIFGRLCAGKNLAFAYTLFSVIGGAIIYYIVMNVVL
                     WLKLPSDDLKLFSAIVVA"
     gene            complement(912..1874)
                     /locus_tag="ACZO9R_04705"
     CDS             complement(912..1874)
                     /locus_tag="ACZO9R_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912402.1"
                     /translation="MKKAHKLISAVLVSTMVLSMTACGSGEKTYKVGILQQLEHPALD
                     QATQGFQDALTKLMGDSVTFDVQNAQGEQANCSSIATTFVSNNYDLILANATTALQAS
                     AAATSTIPVLGTSVTDYATALDIDDWTGATGKNISGTSDLAPLDQQEDMLVELFPDAR
                     NVGILYCSAEPNSVYQATEFGKYLDEDGISHKEYTVADSNDIASVVQSAVSEVDVIYI
                     PTDNTMAGSTEAIDNITRPAGIPVIAGEEGICSGCGVATLSISYYDIGYKAGEMAYDI
                     LVNGKDISTMDIEYAPNVTKEYNADICSDLGITVPDDYVAIGAE"
     gene            2040..2825
                     /locus_tag="ACZO9R_04710"
     CDS             2040..2825
                     /locus_tag="ACZO9R_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HIR0912403.1"
                     /translation="MTGGRAGNAMQADFATGKEKTGRGLRWVAETIVGLVYPPRCPIC
                     DRVTVPECVPCRRCADAVRLAEEPVCKRCGKPLDGERAEFCLDCGKRRHAYCQGKAVF
                     VYQGNIRQSMYRFKYANRREYAAYYAREAAALYQDWVFKNQIEVIVPVPMYRWKKRRR
                     GYNQAETFARALGGELGLPVDAGLVRRVRNTVPQKELNGGQRAANLKNAFQLAADIVK
                     YKKILLVDDIYTTGTTIDTVSEVLLKGGAERIYYICVSIGAGF"
     gene            2853..3254
                     /locus_tag="ACZO9R_04715"
     CDS             2853..3254
                     /locus_tag="ACZO9R_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein"
                     /protein_id="HIR0912404.1"
                     /translation="MEVRNCRSCGRLFNYMGGAPLCPACQKKLEEKFQEVKAFLEENP
                     NSSVETVAEELDVSVKQIRQWIREERLSLSVAGADGIVCETCGKPIRTGRFCEKCKNN
                     MANALAESIHKPEPPQPQETERDKNRMRFLK"
     gene            3408..>3783
                     /locus_tag="ACZO9R_04720"
     CDS             3408..>3783
                     /locus_tag="ACZO9R_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912405.1"
                     /translation="MVNEERLRHLIKMAQFDTNDGKQCKPMTQYARKDYVSMRLLGSF
                     VTGTICYGLLMGMWGLYATEELLAKLNKFDIQGLVMTAVVPYLVFMVIYLGATWVIFN
                     MKYTEGRRKVKKYYNNLKKVNQM"
BASE COUNT          989 a          841 c         1126 g          827 t
ORIGIN      
        1 tgccacaacg atcgcggaga aaagcttcag atcgtcggaa ggaagcttta accataaaac
       61 cacattcatt acaatataat agatgatcgc tccgccgatt acggaaaaca gcgtgtaggc
      121 gaaagcgaga ttcttcccgg cgcagagtct gccgaaaata acttctccga taatgacagc
      181 agcaagaccg atgacgatgg cgccgcgccc catgttgaca tctgccgcac cctgatactg
      241 ggcgtacaga ccgccggaga gaccgaccag accgttggag atcatgagcg caatgacggt
      301 gtgaaggttg gtgttgattc cctgcgcacg cgccatctgc tgattacatc cggttgcgcg
      361 gatggcatgt ccctgctccg tgccgaaata ccaatagaga atcgcaacga ttgcaaaaca
      421 gaacaggatg catgtcgcaa aaatctgtac acgggtgctg ttggagtcgg acaggtagcg
      481 gagcgagacc accagagggt acttgtcaac actgacggcc tggttggaac agcccatgat
      541 gtttaagttg atggaataca gggaaatctg ggttaaaatg cttgccagaa tgcccgggat
      601 gccaagagcg gtatgcagga tgccggtgat gaaaccggcg accatgcctg ccagaaaggc
      661 aaaaataagt gctgcccacg gactcatacc tccgcggatc agcatgacgg caaccgcacc
      721 tccggttgca agagaaccgt ccacggtcag atccgcaaaa tccaacagac ggtaggtaat
      781 atagacgccg agtgccatca gcccccagac catgccctgg gcgaaagcgc cgggcatgga
      841 acggaagagg gcaagcgggt caagcgacat aaaataattc atcaggaaaa acccccttgt
      901 aaggaaaaat attattcggc accgattgcc acgtaatcat ccggaacggt aatgccgaga
      961 tcggaacaga tatccgcatt gtattctttt gtgacattcg gggcatattc aatatccatt
     1021 gtggatatat ctttgccgtt taccagaatg tcataagcca tctcgcccgc tttgtagccg
     1081 atgtcgtaat agctgatgga gagcgtcgcg acgccgcagc cggaacagat tccttcttca
     1141 ccggcgatga cgggaatgcc ggccgggcgt gtgatgttgt cgatcgcttc ggtgctgcct
     1201 gccatggtgt tgtcggtcgg aatgtagatg acgtctactt cgctgacagc actctgcacg
     1261 acagaggcga tgtcgttgga atctgccacg gtgtactcct tgtgggagat tccgtcctca
     1321 tccagatact ttccgaattc cgttgcctgg tacacggagt tcggttctgc ggaacagtag
     1381 aggataccga cattcctggc gtctggaaac agttccacga gcatatcttc ctgctggtct
     1441 aacggagcca gatcggaggt gccggaaata ttttttccgg tggcgccggt ccagtcgtca
     1501 atgtcgagcg cggttgcgta gtccgtcacg gaggttccga gtaccgggat tgtgctggtg
     1561 gccgccgcgg aagcctgcag ggcggtcgtc gcattggcga ggatcagatc gtaattgttg
     1621 gagacaaagg tggtggcaat gctggaacag ttcgcctgct cgccctgtgc gttctgcaca
     1681 tcgaaggtga cggaatcgcc catcaatttg gtcagggcat cctgaaatcc ctgcgttgcc
     1741 tgatccagtg ccggatgctc cagctgctgc aggataccta ccttatacgt tttttctcca
     1801 ctgccgcatg cggtcatgga taagaccatc gtggagacta agactgccga aatcagtttg
     1861 tgtgcttttt tcataagcat ctcctccttt attgcattaa aactttaatg cttttaatag
     1921 acaaaattat aatgaggaaa agtatattcg tcaaccgttc cttaaaataa agaaagaaaa
     1981 aattaagaaa atgtttatgg caaaaaaggg gcggacatgc taggataaag cggaatcaga
     2041 tgaccggggg gagagcggga aatgcaatgc aggctgattt tgcaacagga aaagaaaaaa
     2101 caggacgcgg actgcggtgg gtggcggaga cgatcgttgg cctcgtctat ccgccgagat
     2161 gtccgatctg cgaccgcgtg acggtgccgg aatgtgtccc gtgcagaagg tgcgcggatg
     2221 ctgtacggct tgccgaggaa ccggtgtgca agcgatgcgg caagccgctg gatggcgagc
     2281 gggcggaatt ctgtctggac tgcggaaagc gcaggcatgc gtactgccag gggaaagccg
     2341 tgtttgtgta tcagggaaat atccggcagt ccatgtaccg gtttaaatat gcaaaccggc
     2401 gcgaatatgc ggcgtattat gcgagggagg cggcagcgct ttatcaggac tgggttttta
     2461 aaaatcaaat cgaagtgatc gttccggtgc ccatgtaccg gtggaaaaag cggcggcgcg
     2521 gatacaatca ggccgagaca ttcgcgcggg cgcttggcgg ggagctgggg cttcccgtgg
     2581 acgccgggct tgtgcgccgt gtgcggaaca cggtgccgca gaaggagtta aatggcggac
     2641 aaagagcggc aaatttaaaa aatgcttttc aattagcagc agatatagta aaatataaga
     2701 aaatattact ggtggatgat atttatacga cgggaacaac gatagatacc gtttcggagg
     2761 tcctgctgaa gggcggcgca gagcggatct attatatttg cgtcagcatc ggtgcgggat
     2821 tttgacaggg gaattcagga ggattgagaa gtatggaagt gagaaattgc aggagctgtg
     2881 gacgcctgtt taactatatg gggggagcgc cgctttgtcc ggcgtgtcag aaaaagcttg
     2941 aggagaagtt ccaggaggta aaggctttcc tggaggagaa tccgaattca agtgtggaga
     3001 cggtcgctga ggagctggat gtgtcggtga agcagatcag acagtggatt cgcgaggagc
     3061 gtctgtcact ttccgtggca ggagcagacg gcattgtctg tgagacctgc gggaagccga
     3121 tccgcacggg aagattttgc gagaagtgca agaacaatat ggcgaacgcg cttgcagagt
     3181 cgattcataa gccggagccg ccgcagccgc aggagacaga gcgggataaa aaccgcatgc
     3241 gttttctcaa ataataccaa gagaatcaac agggcctggg tatcgggctc tgttttttgc
     3301 tttgaaaata aatagtgctg tgttataata aaaaaagaat gcataattgc gtgcaatttt
     3361 gccctgcgca ggctggaacg gcagggcgga gaaaggataa ctactgtatg gtaaatgaag
     3421 aacgattgcg ccacctgatt aagatggcac agtttgatac aaatgacggg aaacagtgta
     3481 agccgatgac gcagtatgcg agaaaagatt atgtttccat gcgtctgctg gggagcttcg
     3541 tgacaggaac catctgctac gggcttctga tgggaatgtg ggggctgtat gcgacggaag
     3601 agctgcttgc aaagctgaac aagttcgata tccaggggct tgtcatgaca gcggtagtgc
     3661 cgtatctggt gtttatggtg atttacctcg gagccacgtg ggtaatattt aacatgaaat
     3721 acacagaggg acgccgcaag gtgaagaagt attacaacaa cctgaaaaag gtgaatcaga
     3781 tgt
//
LOCUS       DBMDQJ010000174         3755 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9011_length_3755_cov_1.450739, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000174 DBMDQJ010000000
VERSION     DBMDQJ010000174.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9011_length_3755_cov_1.450739"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="ACZO9R_04725"
     CDS             <1..572
                     /locus_tag="ACZO9R_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912406.1"
                     /translation="LKGICQQITNAHPLGCKNPSFMDISNAKLFGAVPSLVVYMLLLG
                     AAFYYLMRYTSYGRNCYAIGGNYDVAKYSGIKVIPIKWTAFVISGMTATLAGVLLSSR
                     MNTGNSVYGDTTGMLVNCGVVIGGTSFAGGEGGILQSFIGLMAVQMLSTCMSALSVDG
                     FWQKLIEGLMIVAIIAADCYSRKRKRENV"
     gene            592..1455
                     /gene="aroE"
                     /locus_tag="ACZO9R_04730"
     CDS             592..1455
                     /gene="aroE"
                     /locus_tag="ACZO9R_04730"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR0912407.1"
                     /translation="MKEQNYLDHLVGVFGQPVAENPGVVIQDAAFRALGLERWRYLTL
                     DVDKDKLGDAIRGLKALKMRGVNCTIPHKIAVMEYLDELSESARLIGAVNTIVNDNGR
                     LYGDNTDGKGFMMSLQSNGVDVRGKRAVVFGAGGAARAICVEMALAGAVDITIVCRPR
                     GRALGEALVEIVQKNTQTAVRCEDWVGEHSIPAGTDIVVNATPVGLYPNVGELPKIAL
                     DSITPDMFVQDIIPNPTETALVKEMRRRGIPCATGAGMLIEQAALNIEMWTGQKPDRS
                     VMLKALEEALA"
     gene            1392..2189
                     /locus_tag="ACZO9R_04735"
     CDS             1392..2189
                     /locus_tag="ACZO9R_04735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023880.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HIR0912408.1"
                     /translation="MDWAEARPFGDAQGAGGGAGVRDESANARERKAAGLPYVYNDET
                     ILREQYAYQDALAKYNRTLPSEHETRARMLLSLMAEVGESCTIDTPVHANWELRHVHF
                     GRNIYCNAMVSFVDDADIYIGDDCMIAPGTVFATAGHPVNPRLRELRYVYNLPICVGR
                     NVWIGSGVQIMPGITIGDNSVIGAGSVVTKNIPSNVVAVGNPCRVLREIGTHDRKYFY
                     KNFELDLTRNWSIFLCKNPCRIAVWLILQAPSDPSEGACFLKRFGCK"
     gene            2386..2462
                     /locus_tag="ACZO9R_04740"
     tRNA            2386..2462
                     /locus_tag="ACZO9R_04740"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2420..2422,aa:Arg,seq:ccg)
     gene            complement(2573..2731)
                     /locus_tag="ACZO9R_04745"
     CDS             complement(2573..2731)
                     /locus_tag="ACZO9R_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912409.1"
                     /translation="MWFRKQKAEKEERRGRKNQEEQRSKEELSSLEKAKQEYDLWVLA
                     EEYEEYER"
     gene            complement(2734..3402)
                     /locus_tag="ACZO9R_04750"
     CDS             complement(2734..3402)
                     /locus_tag="ACZO9R_04750"
                     /inference="COORDINATES: protein motif:HMM:NF021388.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HIR0912410.1"
                     /translation="MEENISEYDRLDAAQSEFLRTFLTCRGNMKDVQNKIGISYPTAK
                     KRLDELLKALGIADNNVENRKETVNAAMLERADDNSINASDIIKNKLIDCGGIATVRS
                     VSGNSYVIQANADGNSFTCDELPITPPYEYRVFDVIVSLLFRNGGKARKGNGRNYRFG
                     YGDCTEDTIVGCIAKDKGIAEGAYAYDPVFVLSAILDWAGIAHNERGYLELTAEYRTK
                     AEGR"
     gene            complement(3415..3495)
                     /locus_tag="ACZO9R_04755"
                     /pseudo
     CDS             complement(3415..3495)
                     /locus_tag="ACZO9R_04755"
                     /inference="COORDINATES: protein motif:HMM:NF046814.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089-like zinc ribbon domain-containing
                     protein"
     gene            complement(3581..3751)
                     /locus_tag="ACZO9R_04760"
     CDS             complement(3581..3751)
                     /locus_tag="ACZO9R_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012798.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR0912411.1"
                     /translation="MRHTAASLFIASGTDVGTVAGLLGHSQPSTTLDIYTHAFDKNKK
                     AASQIMQNELEI"
BASE COUNT          807 a         1008 c          993 g          947 t
ORIGIN      
        1 tcttaaaggg tatttgtcag cagatcacca atgcccatcc gctcggctgc aagaacccca
       61 gctttatgga catctccaat gcaaagctct ttggcgctgt tccgtctctt gttgtgtata
      121 tgctgctttt gggcgctgcg ttttactatc tgatgcgcta taccagctat ggtcgcaact
      181 gctacgcgat cggcggcaac tacgacgtcg cgaaatactc cggcatcaag gtcatcccga
      241 tcaaatggac agcctttgtt atcagcggca tgactgccac gctggcaggc gttttgctgt
      301 cctcgcgtat gaacaccggc aactctgttt acggcgatac caccggtatg ctggtcaact
      361 gcggcgtcgt catcggcggc acgtccttcg ctggcggcga gggcggcatt ttgcagagct
      421 tcatcggtct gatggcggtg cagatgctct ccacctgtat gtccgcgctg tctgttgacg
      481 gcttctggca gaagctgatc gagggtctga tgatcgtcgc catcattgcg gcagactgct
      541 attccagaaa acgcaaacgc gaaaacgtct gatgcggagc gagggagcag catgaaggaa
      601 cagaattatc tggatcatct ggtcggcgtg tttggtcagc ccgtagcgga gaatcccggc
      661 gtcgtcattc aggatgccgc cttccgggcg ctcggtctgg agcggtggcg gtatctgacg
      721 ctggatgtgg ataaggacaa gctgggcgat gccatccgcg ggctgaaggc gcttaaaatg
      781 cgcggtgtca actgcaccat cccccacaag atcgcggtca tggagtattt ggacgagctc
      841 tccgagagcg cccgtctgat cggcgcggtc aatacgatcg taaacgacaa cggcaggctt
      901 tacggcgaca acaccgacgg caagggcttt atgatgtcct tgcagtccaa cggtgtagac
      961 gtcaggggaa agcgcgccgt agtattcggc gccggcggcg cggcccgcgc catctgcgtg
     1021 gagatggcgc tcgcaggcgc ggtggacatc accatcgtct gccgcccgag ggggcgcgcc
     1081 cttggcgagg cgcttgtgga gatcgtgcag aagaacaccc aaacggcggt acgctgcgag
     1141 gactgggtcg gcgagcacag catccccgct ggcacggaca ttgttgttaa cgcaacgccg
     1201 gtcggtctgt accccaatgt gggagagctg cccaagatcg cgctggactc catcacgccg
     1261 gatatgtttg tgcaggacat catcccgaat cccacggaaa ctgcactcgt caaggagatg
     1321 cgccgccgcg gcatcccctg cgccaccggc gccgggatgc tcatcgaaca agctgcgctg
     1381 aacattgaga tgtggactgg gcagaagccc gaccgttcgg tgatgctcaa ggcgctggag
     1441 gaggcgctgg cgtaagagat gaaagtgcaa acgcgcgtga gcgaaaagct gcgggacttc
     1501 cctatgtgta taacgacgag accattctga gagaacagta cgcctatcag gatgcgttgg
     1561 caaaatacaa tcggacgctt ccgtctgaac acgaaacgcg cgccagaatg ctgctgtcgc
     1621 tgatggcgga ggtgggtgaa agctgcacca ttgacacgcc ggtccatgcc aattgggaac
     1681 tgcgtcacgt tcattttggc agaaacatct actgtaacgc aatggtgtcc tttgtcgatg
     1741 atgcagacat ttatatcgga gacgactgca tgatcgctcc cggcacggtg tttgccacag
     1801 ccgggcatcc agtcaacccc cgcctgcggg agctgcggta tgtgtataat ctgcccatct
     1861 gcgtggggcg aaatgtttgg atcggctccg gcgtgcagat catgcccggc atcaccatcg
     1921 gcgacaacag cgtgatcggc gcaggctccg tggtcaccaa gaatatcccc tctaacgtgg
     1981 tggcagttgg aaatccatgt cgtgttcttc gtgagatcgg cacccatgac agaaaatatt
     2041 tctacaaaaa tttcgagctg gatttgaccc ggaattggag catcttcctc tgtaaaaatc
     2101 cttgccggat cgccgtttgg ctcatactgc aagcgccttc cgatccttcg gaaggcgctt
     2161 gttttttaaa acgctttgga tgcaaatgac cttttagggc atgaaaatta aagaggccac
     2221 agaaacacgc agcttcgagc aaattccggc tgctgcgcgt cactacacag aagcataaaa
     2281 caatgtgtaa gatggcatcg cgcttaccct gcattgccag cgccgtcaac agcatgagcg
     2341 tgactactgt ttgactacca ttttcttact accacaactc aatatgcgcc cgtggcgcag
     2401 ctggatagtg cgtgtgactc cggctcacaa ggtcgcaggt tcgaatcctg tcgggcgtac
     2461 caaaatgccg tgaaatcgta tgatttcacg gcgtttttat ataaactgat caaattttct
     2521 cctttgagac atagttcagt tggatggagc cttttcatgg acaaataaag gtttaccgct
     2581 catattcctc atattcttct gccagcaccc agagatcgta ttcctgtttt gccttttcca
     2641 acgaggacag ttcctctttc gagcgctgtt cttcctgatt ctttctgccg cgccgttctt
     2701 ctttctcggc tttttgtttt cggaaccaca tactcaacgc ccctctgctt ttgtacggta
     2761 ctccgcagtc agttcgagat agccgcgctc gttatgcgcg atccctgccc aatcgaggat
     2821 tgctgacaat acaaatactg ggtcataggc atatgcacct tctgcgatac ctttatcctt
     2881 cgcgatgcag ccgacgattg tgtcctccgt acaatcacca tagccgaatc tatagttccg
     2941 tccgtttcct tttctcgcct ttccgccgtt tcggaacaga agactgacga tcacatcaaa
     3001 cacgcgatat tcatatggcg gggtgattgg aagctcgtca caggtaaagc tatttccgtc
     3061 tgcgtttgct tggatcacat agctgttgcc gctgacggat cgtactgttg cgattcctcc
     3121 gcagtcgatc agcttgttct taatgatgtc agacgcattg atgctgttat catccgctct
     3181 ttctaacata gccgcattca ccgtctcctt tctattctct acattgttgt ccgcgatgcc
     3241 cagcgccttc agcaattcat ccaatcgttt tttcgcagtc gggtatgaaa ttccaatttt
     3301 attttgcaca tccttcatat ttccgcggca ggttaaaaat gtacgcagga actcagactg
     3361 cgcggcatcg agccggtcgt actcggaaat attttcttcc acagaaaact ctgctttgca
     3421 agaggggcag gacagtttgc tgatatttag cctgtctccg catacaggac atacgctggt
     3481 ttttgtaatt ttcacctgtg tgtcacctcg cgtttcgata atatcatttt attttgatct
     3541 tgtcaacatt agaattaaat attataaaaa tctatattat ttagatttcc aattcgttct
     3601 gcatgatctg gctggcggct ttcttgttct tatcgaacgc gtgggtgtag atatccagcg
     3661 tggtcgatgg ctggctgtgt cctaagagtc cggcaaccgt gccaacgtcc gtgccgctgg
     3721 cgatgaacag gcttgccgcc gtgtggcgca ggctg
//
LOCUS       DBMDQJ010000175         3738 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9055_length_3738_cov_1.591696, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000175 DBMDQJ010000000
VERSION     DBMDQJ010000175.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9055_length_3738_cov_1.591696"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="ACZO9R_04765"
     CDS             complement(<1..701)
                     /locus_tag="ACZO9R_04765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912412.1"
                     /translation="MAEKKTAPVILFTYNRPEHTRRTIEALAKNELAQDTELFVFSDA
                     AKKEADRGKVQEIRDYVAQAGGFFKVTLIAREENYGLARNVIEGVTEIIERYGTVIVL
                     EDDLVTNRYFLRFMNDALSRYEKNPKVTGVTGFSHFADTVTLPCESYFHTLTGTSWSW
                     ATWADRWRHFDATCSDWTRMKEDAKLRRRFNYDNTYNFYKIMKDQQTDAGTNSWAIRW
                     YWTNFKRDGYILSPLR"
     gene            complement(726..1649)
                     /locus_tag="ACZO9R_04770"
     CDS             complement(726..1649)
                     /locus_tag="ACZO9R_04770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR0912413.1"
                     /translation="MKKMVITGVNGFIGRHAKEYFEKEYEITGLDLAANYCGDRLPGD
                     TMAYYQCNMSQESAEMAAIFTGVQPDVILHCAGSANVGASVVNPMADLDGNLHSLYQL
                     LLALKSFEKRPQIIFLSSAGIYGNPRQLPVRESDAAAPMSPYGLHKHMAEELCEYYNR
                     IHGYRIRSIRIFSAYGNGLRKQLLWDIYQKYQNTGRIELFGTGEETRDFIHVSDIVRA
                     IELILAYDGPENVINVANGEEVSIRELAEIYAGKLGADADVVSFNGMVKEGDPRNWRA
                     DISLLKKLGYEKHMALSDGIEAYVEWAREQE"
     gene            complement(1680..2825)
                     /locus_tag="ACZO9R_04775"
     CDS             complement(1680..2825)
                     /locus_tag="ACZO9R_04775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912414.1"
                     /translation="MKKILFNGLGNRGWIGGLYYLKNIIFSCLESEKIKKEYRIVVLI
                     DSAHADIFTCFGNRIDLHIYDGTNKAKLALYEMNLIWLHGVKYCYALELNKIGSLFAS
                     RGIFWIPDFQHKTLPEFFQPEELARKDVTDRKMTELPNTLVLSSEDARHDLERFYPEH
                     KCRTQVVHFVSYIEPELRTITPELERGVADKFGLHKKYVYLPNQFWKHKNHVVAVRAI
                     ERIKKRGLLADYDFVFTGNLEDYRNPEYINELKAIMETEPVAGSIRLLGFVERTEQLC
                     IMKNAAFIVQPSLCEGWGTVLEDAKVLDKAVLLSDIPVHREQKNEKCTLFDPHDPDAL
                     ADLIVVTAERADRPEEAAYFAGDMMAGMANMYREAGEYAKALEKIFV"
     gene            complement(2822..>3738)
                     /locus_tag="ACZO9R_04780"
     CDS             complement(2822..>3738)
                     /locus_tag="ACZO9R_04780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR0912415.1"
                     /translation="RKRFDDRYVFLPVGVEKLLAEIKPDLVFAMEYNPTILRAVHWCR
                     KKRIPFISWTDGTLNSEKNIGKVQRLSRSYIIKRAAAFVASSTASKQAQIAYGADEKK
                     CFLSYLTVDIQKYLREKEDYSGRELLYVGSLIQRKGLDLLLPALAETSEDIHLTIVGE
                     GQERELLTEQAGKLGITDRIRFLGYVEGEPLRELYHNADVFILPTREDCFGLVILEAM
                     CASLPVISSKYADGAFDLVAEGENGYIVDPEDTHAFAEKIDQIFADENRLKEMGTRSY
                     ERAHAFAFEEVAKGCIDALCYVMGKAVR"
BASE COUNT          793 a         1140 c          848 g          957 t
ORIGIN      
        1 cgcagcggtg agaggatgta gccgtcccgt ttaaaattcg tccagtacca gcggatcgcc
       61 caggaattcg tccccgcatc cgtctgctgg tccttcataa tcttatagaa attgtaagtg
      121 ttatcgtaat taaagcggcg gcgcagcttt gcatcctctt tcatccgcgt ccagtcgctg
      181 catgtcgcat caaaatgccg ccaacggtct gcccaggtcg cccatgacca gcttgtcccg
      241 gtcagcgtgt gaaaatagga ttcacacggc agtgtgaccg tatccgcaaa atgggaaaaa
      301 ccggtcacac cggtcacctt gggattcttc tcgtagcgcg agagcgcatc attcataaac
      361 cgcaggaaat agcggttcgt cacaagatca tcctccagaa cgatcacggt accatagcgc
      421 tctatgatct ccgtcacacc ctcgatcacg tttctggcaa gcccgtaatt ttcctcccgc
      481 gcaatcagcg tcactttgaa aaagccgcct gcctgcgcca cgtagtcgcg gatttcctgt
      541 actttgcccc gatccgcctc tttttttgcc gcgtcggaga acacgaacag ttccgtgtcc
      601 tgcgccagtt catttttcgc cagcgcttct atggtgcgtc tggtgtgctc cggtctgttg
      661 taggtaaata aaatcaccgg cgccgttttt ttctcagcca tgtctctcct cctgccgatg
      721 tccgtctact cctgctctct cgcccattcc acgtatgctt ctatgccgtc ggaaagtgcc
      781 atgtgtttct catatcccag ctttttcaac agggagatat ccgctctcca gttgcgcggg
      841 tcgccctcct tgaccatgcc gttaaaggat acgacgtccg catccgcgcc cagctttccg
      901 gcatagatct ccgccagctc gcggatcgat acttcctccc cgttcgcgac attgatgacg
      961 ttctccggtc cgtcataggc aaggatcagt tcgatcgcac gtacaatatc ggagacatga
     1021 atgaaatctc gcgtctcctc gccggttccg aacagttcga ttcttccggt gttctgatat
     1081 ttctgataga tgtcccacag cagctgcttg cgcagtccgt tgccgtaagc agaaaaaata
     1141 cggatgctgc ggatgcgata gccgtgaatc cggttgtaat actcgcagag ctcctccgcc
     1201 atgtgcttgt gcagcccata gggggacatc ggcgccgcag cgtctgactc gcgcaccgga
     1261 agctgtctcg gattgccgta gattccggca ctcgagagga aaatgatctg cggtcttttt
     1321 tcaaatgact tcaatgccag aagcagctga tagagactgt gcagattgcc atcgagatct
     1381 gccatcggat tgaccaccga agccccgaca ttggcgcttc ccgcgcagtg cagaatcaca
     1441 tccggctgca cgccggtaaa gatcgccgcc atctccgcgg actcctgcga catgttgcac
     1501 tgatagtagg ccatggtgtc acccggcagc ctgtctccgc agtaatttgc cgccagatcc
     1561 agccctgtga tctcgtattc tttttcaaaa tattcctttg cgtgtcttcc gataaatccg
     1621 ttgacaccgg ttatcaccat tttcttcata ttcatcctcc ggcacaaggc catcttccat
     1681 tacacgaaaa tcttctccaa tgccttcgcg tactctcccg cctcgcggta catgtttgcc
     1741 attcccgcca tcatatctcc tgcaaaatag gcagcttctt ccggccggtc tgccctctct
     1801 gccgtcacca cgatcagatc tgccagtgca tccggatcgt gcggatcaaa gagtgtgcat
     1861 ttttcattct tctgctccct gtgtaccgga atgtccgaca acagcactgc cttatcaagc
     1921 accttggcat cctccagcac cgttccccag ccttcgcaga gggacggctg cacgataaag
     1981 gcggcatttt tcataataca aagctgctcc gtgcgctcca caaagcccaa aagccggatg
     2041 cttcccgcca ccggttcagt ctccatgatc gcttttagct cattgatgta ttccgggttg
     2101 cggtagtctt ccagattgcc ggtaaacaca aaatcataat ccgcgagaag tccccgcttt
     2161 ttgatgcgct cgatcgcgcg caccgcgacc acatgattct tgtgcttcca gaactggttc
     2221 ggcagataga cgtacttttt atgcagtccg aatttgtccg ccaccccgcg ctctaactcc
     2281 ggcgtgatcg tgcgcagttc cggctctata taggagacaa aatgcaccac ctgagtcctg
     2341 catttgtgtt ccggataaaa acgctccaga tcatggcgtg catcctcact gcttaatacc
     2401 agcgtattcg gaagttctgt catcttccga tccgtgacgt ccttgcgcgc cagttcctcc
     2461 ggctggaaga attccggcag cgtcttgtgc tggaaatccg gaatccagaa aatgccgcgt
     2521 gaggcaaaca ggctgccgat cttgttgagt tccagcgcat agcagtattt cacgccgtgc
     2581 agccagatca ggttcatctc gtagagcgcc agctttgcct tgttcgtccc gtcgtagata
     2641 tgcaggtcga tgcggtttcc gaagcaggta aaaatatccg catgtgcgga gtcgatcaat
     2701 accacaatgc ggtattcctt ttttatcttc tcactctcca gacagctgaa tatgatattt
     2761 tttaagtagt acaaaccgcc gatccatccg cggtttccaa gtccgttgaa caatattttt
     2821 ttcatctgac agcctttccc atcacatagc agagagcatc gatacagccc tttgccacct
     2881 cttcaaatgc aaatgcatgc gcgcgctcgt agctgcgcgt tcccatctcc ttaagacggt
     2941 tttcgtccgc aaaaatctga tcgatcttct ctgcaaacgc atgtgtatcc tccgggtcga
     3001 cgatgtagcc gttctccccc tccgccacca ggtcaaacgc accgtcggcg tactttgagg
     3061 agatcaccgg aagcgacgca cacatcgcct ctaagatcac gagtccgaaa caatcctctc
     3121 tggtcggcag gatgaagaca tccgcgttgt ggtacagttc gcgcagcggc tccccctcca
     3181 catatcctaa aaagcggata cggtcggtaa ttccaagctt tcccgcctgt tccgtaagaa
     3241 gctcccgctc ctgtccctca ccaacaatcg taaggtgaat gtcctcactc gtctctgcca
     3301 gcgccggaag cagaaggtcc agcccctttc tctggatcag gcttcccaca tacagaagtt
     3361 cccttccgct gtagtcttcc ttttcgcgca gatatttctg aatgtcgacc gtcagatagg
     3421 agagaaaaca tttcttctca tccgccccgt atgcaatctg cgcctgcttc gatgcggtgc
     3481 tgctcgccac gaacgccgcc gcacgcttta tgatgtaact ccgggaaagc cgctgcacct
     3541 tcccgatatt tttttctgaa ttcaaggttc catccgtcca gctgataaat ggaatcctct
     3601 tctttctgca ccaatgtacc gctctaagga tcgtcgggtt gtattccatg gcaaatacaa
     3661 gatccggctt tatttctgcc agcagcttct ccacgccgac cggaaggaac acataccggt
     3721 catcaaaacg cttgcgga
//
LOCUS       DBMDQJ010000176         3728 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9092_length_3728_cov_1.727323, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000176 DBMDQJ010000000
VERSION     DBMDQJ010000176.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9092_length_3728_cov_1.727323"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2118)
                     /gene="gyrA"
                     /locus_tag="ACZO9R_04785"
     CDS             complement(<1..2118)
                     /gene="gyrA"
                     /locus_tag="ACZO9R_04785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR0912416.1"
                     /translation="MDDKIFDQIHDVDLKKTMESSYIDYAMSVIAQRALPDVRDGLKP
                     VQRRVLYSMIELNNGPDKPHRKCARIVGDTMGKYHPHGDSSIYGALVNMAQDWSFRYP
                     LVDGHGNFGSVDGDGAAAMRYTEARLSKISMEMLADIGKDTVDFVPNFDETEKEPTVL
                     PSRFPNLLVNGTTGIAVGMATNIPPHNLREVINGVVKIIDNQVLEDRGTDIEELLTIV
                     KGPDFPTGGTILGTAGINEAYRTGRGKVRVRAVTSIEPMQNGKNRIVVTELPYMVNKA
                     RLIEKIAELVRDKKIDGITDLRDESDRQGMRICIELRRDVNPNVVLNLLYKHTQLQDT
                     FGVIMLALVDNQPKVLNLYEMLNYYLIHQEEVVTRRTKYDLNKAEERAHILQGLLTAL
                     DNIDEVINIIRSSKNTQEAKDRLMERFALTDVQAQAIVDMRLRALTGLEREKLENEYA
                     ELMERITELKAILADKKKLLGVIREEILLIADKYGDDRRTSIGFDEYDISMEDLIPVT
                     NTVITMTKLGYIKRMSLDNFRAQNRGGKGIKGMETIDDDYIEELLMTTSHHYLMFFTN
                     TGRVYRMKAYEIPEASRTARGTAIINLLQLQPGEKITAVIPIREYTEGHFLFMATKKG
                     IVKKTPITDYANVRKTGLAAINLREDDELIEVKKTDNNQDIFLVTKYGQCIRFKETDV
                     RKTGRTSMGVIGMNLTDGDEVVGM"
     gene            complement(2168..>3728)
                     /locus_tag="ACZO9R_04790"
     CDS             complement(2168..>3728)
                     /locus_tag="ACZO9R_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008723096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR0912417.1"
                     /translation="SCVNALSDSLIAEVHREGKIYEQKYSKGKPDEPVKVIGKCSAEK
                     HGTKVTFHPDPTIFEETVYDYDTLKQRLRETAFLTKGLRIVLSDARVDPVHTHEFHYA
                     GGIKEFVTYLNKSKEALYPEVIYCEGTRDGVYVEVAMQHNDSYNDATYSFVNNIITPE
                     GGTHLAGFRNALTKTFNTYARANKILKDTEPALTGDDIREGLTAIISIKIEEPQFEGQ
                     TKQKLGNSEARGAVDSVVSEQLTYFLEQNPTVAKNICEKSLLAQRAREAARKARDLTR
                     RKTALEGMSLPGKLADCSDKDPKNCEIFIVEGDSAGGSAKTARSRATQAILPLRGKIL
                     NVEKARLDKIYGNAEIKAMITAFGTGIHEDFDISKLRYNKIIIMTDADVDGAHIATLM
                     LTFLYRFMPELIKQGHVYLATPPLYKIEKNKNVWYAYDDNELNSILTEIGRDGNNKIQ
                     RYKGLGEMDAEQLWETTMDPEKRILKRVMIDEENSSEIDVTFTTLMGDKVEPRREFIE
                     ENAKYVQNLDI"
BASE COUNT          806 a         1011 c          841 g         1070 t
ORIGIN      
        1 catgccgacg acctcatctc cgtctgtcag gttcatacca atgacgccca tggatgttct
       61 gcctgttttt cttacgtctg tctccttgaa ccggatacac tgtccgtatt tggtgacaag
      121 gaaaatatcc tgattattat cggtcttctt cacctcgatc agctcgtcgt cctcgcgcag
      181 attgatggct gcaagacctg tctttctcac atttgcataa tcggtaatcg gtgtcttctt
      241 cacgatacct ttctttgtcg ccatgaaaag gaaatgtccc tcggtgtact cccggatcgg
      301 aatgaccgca gtaatcttct cgcccggctg cagctgaagc aggttgatga ttgcggtacc
      361 gcgcgcggtt ctgcttgcct ctggaatctc ataagccttc atgcggtata cacggccggt
      421 attcgtgaag aacatcaggt aatggtgcga tgtcgtcatc agaagttcct cgatgtaatc
      481 gtcatcaatg gtctccattc ccttgattcc cttgcccccg cggttctgcg cacggaaatt
      541 gtcaaggctc atacgcttga tatatccaag cttcgtcatg gtaatgacgg tattggtaac
      601 cggaatcaaa tcctccatgg aaatatcata ttcgtcaaaa ccgatggatg ttcttctgtc
      661 atccccgtac ttgtcggcga tcagaagaat ctcctcacgg atgacgccga gcagcttttt
      721 cttgtcggca agaattgcct tcaactctgt gatgcgctcc atcaattccg cgtattcgtt
      781 ctccagcttc tcgcgttcca gacctgtcag cgcacgcaga cgcatatcca cgatcgcctg
      841 tgcctgtacg tccgtcagcg caaaacgctc cattaagcgg tcttttgctt cctgggtgtt
      901 cttgctgctg cggataatgt tgataacttc atcaatatta tcaagtgctg ttaataatcc
      961 ctgtaagatg tgagcacgtt cttccgcttt gttcagatca tatttcgttc ttctggtgac
     1021 aacttcttcc tgatggatca ggtaatagtt gagcatctcg taaaggttta agaccttcgg
     1081 ctggttgtca acgagtgcca gcataataac accgaacgta tcctgaagct gtgtatgctt
     1141 gtacagaagg ttcagcacga cattcgggtt cacatcacgg cggagttcga tgcagattct
     1201 cataccttga cggtcggact catcccgcag atcggtgatg ccgtcgattt tcttatctct
     1261 gacaagctcc gcaatcttct cgatcagacg cgccttattt accatgtacg gaagctccgt
     1321 cacaacaata cggttcttgc cgttctgcat cggctcaatg ctcgtcaccg cgcgtactct
     1381 gacttttccg cgtccggtcc ggtatgcctc gttgattccg gcggttccaa gaatcgtacc
     1441 tccggtagga aaatccggtc ccttgacgat cgtcaaaagc tcctcgatat cggttccgcg
     1501 gtcttccaac acctgattat caatgatctt taccacaccg ttaataacct cgcgcagatt
     1561 gtgcggcgga atattggtcg ccattccgac agcgataccg gtcgtaccgt tgactaacag
     1621 attcggaaaa cgggacggaa gcactgtagg ctctttttcc gtctcatcga agttcggaac
     1681 aaaatcaacc gtatcttttc cgatgtccgc aagcatttcc atggaaatct tggaaagtct
     1741 tgcctcagtg tatcgcatag cagcagcgcc gtcaccatcg acggaaccaa aatttccatg
     1801 tccgtcgaca agcggataac ggaatgacca gtcctgtgcc atattgacga gtgctccgta
     1861 gattgagctg tcgccgtgcg gatggtattt acccatcgta tcaccgacga tacgcgcaca
     1921 tttacggtgc ggcttgtccg gtccgttgtt cagctcaatc atggaataga ggacacgcct
     1981 ctgtaccggc ttcaaaccat ctcttacatc cggcagcgca cgctgtgcaa tgacactcat
     2041 ggcatagtcg atgtaggaac tttccattgt tttcttcaga tccacatcat gtatctggtc
     2101 aaaaatcttg tcatccatag gatttttctc catttctatc aattacatac tctgtacagt
     2161 ccgtctgtca gatatcaaga ttctgtacat actttgcatt ttcctcgata aattcacggc
     2221 gcggctctac cttgtctccc atgagcgtcg taaatgtcac atcaatctca gaagaattct
     2281 cctcatcaat catgacacgc tttaagatac gcttctccgg atccatggtc gtctcccaga
     2341 gctgctcggc atccatctca ccaagtcctt tgtatcgctg gatcttgttg ttgccgtcgc
     2401 ggccgatctc tgtcagaatg ctgtttaatt cgttatcatc gtacgcatac cacacgttct
     2461 tgttcttctc gatcttatag agcggtggag tagccagata gacatgtccc tgcttaatca
     2521 gctccggcat gaagcggtac aaaaaggtca gcatcagcgt tgcaatgtgt gcaccgtcga
     2581 catcggcatc agtcataatg atgatcttat tatagcggag cttactgatg tcaaaatctt
     2641 catgaatacc ggtaccaaac gcggtaatca tcgctttgat ctcagcattg ccataaatct
     2701 tgtctaatct tgccttctcc acattcaaaa tcttaccgcg gagcggcagg atcgcctgcg
     2761 ttgcacggct tctcgccgtc tttgcggaac cgccggcgga atctccctct acgataaaga
     2821 tctcgcagtt cttcgggtct ttgtcggaac agtcggcaag cttgcccgga agagacattc
     2881 cctcgagggc ggtctttctt ctggtgagat cacgcgcttt tctcgccgct tccctcgcgc
     2941 gctgcgcgag cagtgatttt tcacagatgt tcttcgccac cgtaggattc tgctccagga
     3001 agtaggtgag ctgctcgctg acaaccgagt caacggcgcc tcgcgcctcg ctgttgccga
     3061 gcttctgctt cgtctgtccc tcgaactgcg gctcctcgat cttgatgctg atgatcgccg
     3121 tgagtccctc gcggatatcg tctccggtga gcgccggctc cgtatccttt aaaatcttat
     3181 tcgcacgcgc ataggtattg aaagtcttcg tcaatgcatt acggaaaccg gcaagatgtg
     3241 taccgccttc cggtgtgatg atattattga caaagctgta agtcgcatca ttgtaggaat
     3301 cgttgtgctg catggcaact tccacataga ctccgtccct ggttccctcg cagtagatga
     3361 cttccggata gagcgcttcc ttgctcttgt tcaagtaggt cacaaattcc ttgataccgc
     3421 ctgcatagtg gaattcgtgt gtatgcaccg gatccactct ggcgtcggag agcacaattc
     3481 ttaagccttt tgttaaaaag gcggtctcgc gcagtctctg ttttaacgta tcgtaatcat
     3541 agacggtttc ctcaaagatc gttggatccg gatggaaggt aaccttcgtt ccgtgttttt
     3601 cggcagaaca cttaccgata accttaacag gttcatccgg cttgcccttg gaatatttct
     3661 gctcatagat cttgccttct ctgtggactt ccgctataag gctgtcggac aatgcgttga
     3721 cgcaggat
//
LOCUS       DBMDQJ010000177         3721 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9116_length_3721_cov_1.524586, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000177 DBMDQJ010000000
VERSION     DBMDQJ010000177.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9116_length_3721_cov_1.524586"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(152..1579)
                     /locus_tag="ACZO9R_04795"
     CDS             complement(152..1579)
                     /locus_tag="ACZO9R_04795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912418.1"
                     /translation="MKRTLYPKLLAGYLMYGLIGFLIITTFTYHITFAFVEKKEAASL
                     YRESALISSNYAGNYFSKTMTLDEIQTQLSTLSTYLSSEIWIVDTHGNIILNTAAPGC
                     DPTPVPGFNITDFGSRYYQTGTFYNQFTSEMLSVFSPITVNYKVRGYVVIHKPTSSLV
                     SYANGLVAIAYETLGLLFLAAFVVLILFTYVVYIPIRKITKAADEYAAGNFEKKIDVH
                     SNDEIGYLAASLNYMANELNTLEDDQKKFVSNVSHDFRSPLTSIKGYVEAMLDGTIPV
                     EMQDKYLNIILFETERLNKLTKSLLELNKFGSHGTMLDITDFDINHTIRMTVQTFEGR
                     CMEKRITFNLILTGEKLFVSADMSKIEQVLYNLIDNAIKFSHTDSAITIETTEKNDKI
                     FVSVKDTGIGIPGDSIKKIWERFYKTDLSRGKDKKGTGLGLSIVKEIVQAHGENINCI
                     STEGVGTEFIFTLPLAKKLSHQAGS"
     gene            complement(1576..2280)
                     /locus_tag="ACZO9R_04800"
     CDS             complement(1576..2280)
                     /locus_tag="ACZO9R_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0912419.1"
                     /translation="MAAKQKILIVDDDNNIAELISLYLTKECFDTRIVNDGEEAMIAF
                     DQYNPNLILLDLMLPGMDGYQVCREIRAKANVPIIMLSAKGEIFDKVLGLELGADDYM
                     MKPFDSKELVARVKAVLRRYQPSKPEEPAAELKCVKYTDLVVNLSNYSVIYMGQAVDM
                     PPKELELLYFLAASPNQVFTREQLLDHIWGYEYIGDTRTVDVHVKRLREKIKDHANWS
                     LATVWGIGYKFEVRDL"
     gene            complement(2311..>3721)
                     /locus_tag="ACZO9R_04805"
     CDS             complement(2311..>3721)
                     /locus_tag="ACZO9R_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HIR0912420.1"
                     /translation="DFRLLIVTGPNTGGKTVSLKTVGLLTLMGQSGLHIPASERSELG
                     IFDEVFADIGDEQSIEQSLSTFSSHMVNIIRILEQVNDRSLVLFDELCAGTDPTEGAA
                     LAISILSKLHLYGARIMATTHYSELKVYALSTPGVENACCEFDVATLSPTYRLLIGIP
                     GKSNAFAISEKLGLPTDLIADAKGRISKSEGDFEDLIADLEKSRSTIEREQLEINQYK
                     AEIESLKEKLEQKQERLDSSRDKILREANEQAYNILKEAKDVADETIRNFNKYGKAGA
                     PVSEMEKERTKLRGKMDKAAQKMSEQKKASVPNHNVPKKLRIGDSVKVISMNLKGTVH
                     SLPNARGDLYVQMGILRSLVNINDLILLEEDAAPGTKKFQKTSAGKIKMSKSASVSTE
                     INLIGKTTDEAIPLLDKYLDDAYLAHLPSVRIVHGKGTGALRNAVQAHLKRLKYVKSF
                     HLGEFGEGDAGVTIAEFKD"
BASE COUNT          820 a          939 c          994 g          968 t
ORIGIN      
        1 gggagagtcc aaacgtggaa gagacacgga atcagatgca gtcatccggc tggaagaaat
       61 acacacattt ttctccaacg aatgtttcat atgcagttcc agccgaaatc tgtatcaagt
      121 gcttcctgta atataactta tcaccccaaa gtcaactgcc cgcctgatgg gacagcttct
      181 ttgctaacgg cagggtaaag ataaattccg ttcccacgcc ttccgtgctg atacagttga
      241 tgttttcgcc gtgtgcctgg acaatctcct ttacgatgga gagaccaagc ccggttcctt
      301 ttttgtcctt gccgcgggag agatccgtct tgtagaagcg ctcccagatc tttttgatgc
      361 tgtcgcccgg gatgccgatg ccagtgtcct tgacggagac gaagatcttg tcgttcttct
      421 ctgtggtctc gattgtgatt gcggagtccg tgtggctgaa tttgattgca ttgtcgatca
      481 agttgtagag cacctgctcg atcttactca tatcggcgga gacaaaaagc ttctcgccgg
      541 ttaagatcag gttgaaagta atgcgctttt ccatgcagcg cccctcgaac gtctggactg
      601 tcatgcggat cgtatgattg atgtcgaaat ccgtgatgtc cagcatcgtg ccgtgtgagc
      661 cgaatttgtt taactcaaga aggctcttgg tcagcttgtt cagccgctcg gtctcaaaga
      721 ggatgatgtt taaatatttg tcctgcatct ctacgggaat ggtgccgtcg agcatcgctt
      781 ccacataacc tttgatggaa gtgaggggcg aacggaaatc atgcgagacg ttagagacga
      841 actttttctg gtcgtcctcc agggtattca gttcgttcgc catgtagttg agcgaggcgg
      901 cgagatagcc gatctcatca ttggagtgaa catcaatctt tttttcaaag ttgccggcgg
      961 catattcgtc cgccgctttc gtaatcttgc ggatcggtat gtataccaca taggtaaaca
     1021 gaatcagtac cacaaaggct gccagaaaca aaagcccaag cgtctcataa gcgatggcga
     1081 caagtccgtt cgcgtaggac acgagactgc tggtcggctt gtggatgacc acataaccgc
     1141 gcactttgta gttgacggtg atcggcgaga aaacacttag catctctgac gtaaactggt
     1201 tataaaacgt gccggtctga taatagcggc ttccgaagtc ggtaatgttg aagccgggaa
     1261 ccggtgtggg atcacagccc ggcgccgccg tgttcagaat gatgttgccg tgcgtgtcca
     1321 caatccagat ttcactggag agataggtgc ttaaggtgga aagctgtgtc tgtatctcgt
     1381 ccaacgtcat ggtcttggaa aaataattcc cggcatagtt ggaggagatc agggcagatt
     1441 cccggtagag gctggcagcc tccttttttt ccacaaaggc gaacgtaatg tggtaggtaa
     1501 aagttgtgat gatgagaaaa ccgatcagac cgtacatcag atagccggcg agcagtttgg
     1561 ggtaaagggt acgtttcata ggtcacgaac ctcgaatttg tagccgatgc cccagaccgt
     1621 tgcgaggctc cagttggcat gatccttgat cttctcgcgg agacgcttca cgtgaacgtc
     1681 cacggtacgt gtgtcgccga tgtattcata gccccagatg tgatccagaa gctgctctct
     1741 tgtaaaaacc tgattcggcg aagccgcaag gaaatataaa agttccagtt cctttggcgg
     1801 catgtcgacg gcctgcccca tgtagatgac ggagtagttc gagagattga ccaccagatc
     1861 cgtgtacttg acgcatttta actctgcagc aggttcctcc ggctttgacg gctgatagcg
     1921 gcgcagcact gccttgacgc gcgcgacaag ctccttggag tcgaatggct tcatcatgta
     1981 atcgtccgcg ccgagctcca gaccgagtac cttgtcaaaa atctcgcctt tggctgacag
     2041 cataatgatc ggaacatttg ccttcgcacg gatctcgcgg cagacctgat agccgtccat
     2101 gccgggaagc atcagatcaa gcaggatcag attcggattg tactgatcaa aagcgatcat
     2161 ggcttcctcg ccgtcattga cgatccgggt atcgaaacat tcctttgtga gatacaggga
     2221 aatcagttct gcgatattat tgtcatcatc tacgatgaga attttctgtt ttgctgccat
     2281 atcatcatct cctcattttt attttaaata tcagtcctta aactcggcaa tcgtgacacc
     2341 ggcatcgccc tcgccgaact cgccgaggtg gaatgatttg acatatttta agcgctttaa
     2401 atgagcctgc accgcgttgc ggagtgcgcc cgtgcctttt ccgtgaacga tgcgcacgga
     2461 cggcagatgc gccagataag cgtcgtccag atacttgtca agaagcggaa tggcctcgtc
     2521 ggtcgtcttg ccgatgaggt tgatctctgt ggagacggag gcggatttgc tcatcttgat
     2581 cttgccggca ctggtcttct ggaacttctt ggtgcccggt gcggcatcct cttccagaag
     2641 gatcaggtcg ttgatgttga ccagagagcg cagaattccc atctgcacat acaggtcgcc
     2701 cctggcattc ggaagggaat gaacggtgcc ttttaagttc atgctgatga ctttgacgct
     2761 gtcgccgatg cgcagcttct tcggaacgtt gtggttcggt acggaagctt ttttctgctc
     2821 ggacatcttc tgtgcggctt tgtccatctt gccgcgcagc ttggtacgtt ctttttccat
     2881 ctcgctgacc ggtgcgcccg ccttgccgta tttgttgaaa ttgcggatcg tttcatcggc
     2941 gacatccttg gcttctttta agatattgta tgcctgctcg tttgcctcgc gcagaatctt
     3001 gtcgcggctg gaatccagac gctcctgctt ctgctccagc ttttccttta aggactcgat
     3061 ctcggctttg tactggttga tctctaactg ctcccgctcg atcgtgctgc ggctcttctc
     3121 aagatctgcg atcaggtctt caaaatcgcc ctcactcttg ctgatgcgcc ccttggcgtc
     3181 ggcaatgagg tccgtcggaa gaccgagctt ttcagagatg gcaaacgcgt tgctcttgcc
     3241 cggaataccg attaagagac ggtaggtcgg acttaacgtc gccacgtcga actcgcagca
     3301 ggcattttcc acgcctggtg tggagagggc atagaccttg agctcgctgt aatgcgtcgt
     3361 agccataatg cgtgctccgt aaaggtggag cttcgagaga atggagatcg caagcgcagc
     3421 gccctcggtc ggatcggttc cggcacagag ctcatcgaaa agtaccagag aacggtcatt
     3481 gacctgctct aagatacgga tgatgttgac catgtgggag gagaacgtac tgagcgactg
     3541 ctcgatactc tgctcgtccc cgatgtcggc gaacacttcg tcgaaaatac cgagttccga
     3601 gcgctcggac gccggaatat gcaggccgga ctgtcccatc agcgtcagca gtccgacggt
     3661 ttttaaggat accgtcttac cgccggtgtt cggaccggtc acgatcagca ggcggaaatc
     3721 c
//
LOCUS       DBMDQJ010000178         3717 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9128_length_3717_cov_1.642976, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000178 DBMDQJ010000000
VERSION     DBMDQJ010000178.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9128_length_3717_cov_1.642976"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..86
                     /locus_tag="ACZO9R_04810"
     CDS             <1..86
                     /locus_tag="ACZO9R_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HIR0912421.1"
                     /translation="NMWEAVFADVGVSVIAILNSMRALKTE"
     gene            699..1448
                     /locus_tag="ACZO9R_04815"
     CDS             699..1448
                     /locus_tag="ACZO9R_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912422.1"
                     /translation="MIVCNNCGASYEDDEPRCPYCGGDNFGRSVQMHEDAVNELKREK
                     RQWEEKPQRMAKTGMSLTAKILIVVIVAGLLLSAAAFIGIRIHAAASGSREQAMQEKL
                     EKMYQQQDYSGICTYLEKHNELYDQAFRKYRLVEKLEDYTANYVITPDGQYLEQLIRE
                     GRAEELDDVKYITDALCICQESEDADYKYEEQEAVTYYREYCYTYLEEHYALTEEEIK
                     EVMDGYDPSDKDNQSNLERMMQERAFSHLTE"
     gene            1469..2230
                     /locus_tag="ACZO9R_04820"
     CDS             1469..2230
                     /locus_tag="ACZO9R_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912423.1"
                     /translation="MICKKCGYDYPAKKLKCPYCGEVNPLGEKWKNEENLARKETLLT
                     KARVIHSMPLYVADKVMNVILLVITAVTALTFLVLAIVFGLEGIHITRQHNQASVQEA
                     EELLVAGDYTGLYDYLKSHQVYGQEGFKKYTERVRLYDDDQAFMRDLFRIQEVLDWPA
                     GQQISPNDVKYVLWAGKNILGRETGDSYRKLEYEENKEYYNDMKQDVAAALLGTFGMS
                     EEELSELMGMERDSEKVDELVRRIFEREGWEYEED"
     gene            2217..2741
                     /locus_tag="ACZO9R_04825"
     CDS             2217..2741
                     /locus_tag="ACZO9R_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912424.1"
                     /translation="MKKIKNLKMMWKFSDETGNYEVSMPMLAVKAAGCVIALFFVFSV
                     LWALIATAVDEGKYSGDAYHLDWCERKYIERNYSGLYNTLDLYGLTSDKYASYWEMVN
                     GYRDHTLYDAYRALGETGIDEVSYETENGTATLSIPVEEKRVFYGRKVLDNASTCEFE
                     ENQRYLTKFADEVE"
     gene            2874..3305
                     /locus_tag="ACZO9R_04830"
     CDS             2874..3305
                     /locus_tag="ACZO9R_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR0912425.1"
                     /translation="MDKIYVVYWSQSGNTQAMAEAVGAGITAAGKEAAVVDVSSVSTD
                     ELKNAKAFALGCPAMGAEVLEEGEMEPFVTEVEGFAAGKTIGLFGSYGWGDGQWMRDW
                     VDRMSSAGANVLNGEGVICKEAPDDEAVAACTDLGKQLAAV"
     gene            3308..3589
                     /locus_tag="ACZO9R_04835"
     CDS             3308..3589
                     /locus_tag="ACZO9R_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HIR0912426.1"
                     /translation="MSVVIVGGHDRMVCQYKKICKEHNCKAKVFTQMPAKLGNQIGSP
                     DLIILFTNTVSHKMVRCAVAEAERCNADVVRSHTSSGSALTEILERVCG"
BASE COUNT         1060 a          698 c         1099 g          860 t
ORIGIN      
        1 cgaacatgtg ggaggcagta tttgcagatg tcggagtatc tgtcattgca atcttaaatt
       61 ccatgcgcgc actaaagacg gagtagaaat aaaaattata gaacctatga acgaaaaaga
      121 gggactgtcg cttgaagcag atggtccctt ttgtcgtgag aagtaatata tcaaaaagat
      181 catgcctttc gatttctgat ggaaatgcag atggaatatt cgtttgagaa aaatgtgagc
      241 atttcttcca gctgagtgtt tgcatcggat tccgtgtctc ttccacgttt ggacaatcca
      301 cggagtgtgt ccgacaagcc acagacaatg agcggattcc ctggtgccgc aagggacact
      361 agggaatcct aaaattgtct gcgggttgcg gacacacccg cggagtgctc caaacttgga
      421 aagacacgga tcatcgaaga tgcaaacatt cggctggtta gaaatgccac attttttgaa
      481 ccgaatttcc agatgtattc catcagaaat cggtaggatg tgcttctttt tctattactt
      541 ctcactccaa aaggggctgt ccgtttcaag cgactgcccc gcttttttgg ttcataggaa
      601 aatgcttcct atacaggaaa aaactgatat aatagacaaa gaataacaca gggtgcctca
      661 gggagcggat ttctgcggag gcaccggagg gagaaacgat gatcgtatgt aataactgcg
      721 gtgcgtctta tgaagatgac gagccgcgtt gtccatattg cggtggcgat aattttggaa
      781 ggtccgtaca gatgcatgag gatgccgtca atgagctgaa acgcgagaaa cggcagtggg
      841 aggagaagcc gcagcggatg gcgaagaccg ggatgtccct gaccgcgaag attttgattg
      901 ttgtgatcgt ggcagggctt ttgctcagtg ctgcggcgtt tattggcata cgcattcacg
      961 cggctgcttc cggcagcagg gagcaggcaa tgcaggaaaa actggagaag atgtatcagc
     1021 agcaggacta cagtggaatc tgcacgtatc tggaaaagca taatgaacta tatgatcagg
     1081 cttttcgcaa ataccggctg gtggagaagc tcgaagacta taccgcgaat tacgtcataa
     1141 ccccggacgg gcagtatctg gaacagctta tccgggaggg aagagcggaa gagctggatg
     1201 atgtgaagta tatcacggat gctctttgca tctgtcagga gagcgaagat gctgattaca
     1261 agtacgaaga gcaggaagcg gtcacttatt accgggaata ttgttatact tatttagagg
     1321 agcattatgc gctgacagag gaagagataa aggaagtgat ggacgggtat gatccatcag
     1381 acaaagataa tcagagcaat ctggagcgga tgatgcagga gcgggcattt tcccatctta
     1441 cggaataaaa gcgagggcgt gtgcgaagat gatatgtaaa aaatgcggat atgattatcc
     1501 ggcgaaaaaa ctgaaatgcc catattgtgg agaggtaaat ccgcttggcg aaaaatggaa
     1561 aaatgaggaa aatctggcgc gaaaggagac actgcttacg aaagccaggg tgattcactc
     1621 catgccgctg tatgttgcag ataaggtgat gaatgtgatt ctgctggtga ttacggcagt
     1681 gacagcactt acatttctcg tgctggcgat tgtgtttggg ctggagggga ttcacattac
     1741 ccgccagcac aaccaggcat ctgtgcagga ggcagaggaa ctgcttgtgg cgggagatta
     1801 tactggtctt tacgattatc tcaaatccca tcaggtatat ggacaggagg ggtttaaaaa
     1861 atatacagag cgcgtccggc tgtatgacga cgatcaggca tttatgagag atctgttccg
     1921 catccaggag gtgttagact ggccggccgg gcagcagatc agtccgaacg atgtaaagta
     1981 tgtactctgg gcgggaaaga atatcctggg aagagagacg ggtgattcct accgcaagct
     2041 ggaatatgaa gaaaacaagg aatattacaa tgacatgaaa caggacgtgg cagcggcact
     2101 gcttggcacc tttggaatgt cagaggaaga actgtcagag ctgatgggca tggaacgtga
     2161 ttcggagaag gtggacgaac tggtacgccg gatctttgag agagagggct gggaatatga
     2221 agaagattaa gaatctgaaa atgatgtgga aattttcaga tgagaccgga aactatgagg
     2281 taagtatgcc gatgctggca gtaaaggcag caggatgtgt gatagcgctg tttttcgtgt
     2341 tctcggttct gtgggctctg attgctaccg ctgtggatga gggaaaatat tccggcgatg
     2401 cctatcatct ggactggtgt gagcggaagt atatagaacg taattacagt ggactatata
     2461 atacgttgga tttgtatgga ctaaccagtg acaagtacgc ttcctattgg gagatggtga
     2521 acggatacag ggatcacacg ctgtatgacg catatcgggc acttggtgag acggggattg
     2581 acgaagtctc ctacgagacg gagaacggca cggcgacact gtcgatccct gtggaggaaa
     2641 agcgcgtttt ctacgggcgc aaggtgcttg acaatgcgag tacctgcgaa tttgaggaga
     2701 atcagcgcta cctgacgaag ttcgcggatg aagtggaata gcagttttct aatgagaaaa
     2761 atatttcatt tactgcactt gacgaaaccc ttcttattta gtatagtata gctaaagtta
     2821 gttaagacta acttacatag cagaatacag agatatagca ggagggaaaa gtgttggata
     2881 agatttatgt agtatactgg tcacagtcag gaaatacaca ggcgatggca gaggcagtcg
     2941 gtgccggaat cactgcagca ggtaaggaag ccgcagtggt ggacgtaagc agcgtctcta
     3001 cagatgaatt aaagaatgca aaggcatttg cactcggatg cccggcaatg ggggctgagg
     3061 ttcttgagga gggcgagatg gagcctttcg taaccgaggt ggaaggattt gcggcaggca
     3121 agaccatcgg tctgttcggt tcctatggct ggggagacgg gcagtggatg cgtgactggg
     3181 tagaccgcat gtcatccgca ggtgcgaatg tattaaacgg cgaaggcgtg atctgtaagg
     3241 aggcaccgga cgatgaggct gtggctgcct gcactgatct cggtaagcag cttgcggcgg
     3301 tgtagttatg agcgttgtga ttgtaggtgg acatgaccgc atggtctgcc agtacaaaaa
     3361 gatctgtaag gaacataact gtaaagcgaa ggtatttaca cagatgccgg caaagctcgg
     3421 gaaccagatc ggatcaccgg atctgatcat tctcttcacc aacaccgtat cacataagat
     3481 ggtacggtgt gcggtggcag aggcggagcg ctgcaatgcg gatgtggtga gaagccatac
     3541 cagcagcgga agtgcactta cagagattct ggagagggtt tgcggataaa taaaagagac
     3601 tgccgcatga cggcagattc ccaaaagatc ctggctggtc cggttggaca ggccgggatt
     3661 tttttgatga gaaaaataac acaataaaac acttgcaata tacccatagg ggggtat
//
LOCUS       DBMDQJ010000179         3696 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9188_length_3696_cov_1.368289, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000179 DBMDQJ010000000
VERSION     DBMDQJ010000179.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9188_length_3696_cov_1.368289"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="ACZO9R_04840"
     CDS             <1..271
                     /locus_tag="ACZO9R_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018684.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HIR0912427.1"
                     /translation="ELTEYDTEQIQIEIYQLWDDELNYLWKYLKETVDEQTMQKLLLE
                     QREWIEKKEQTIQTLQNNDGESLTYPYQAEITRKRVYELTEYIQE"
     gene            383..1210
                     /locus_tag="ACZO9R_04845"
     CDS             383..1210
                     /locus_tag="ACZO9R_04845"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR0912428.1"
                     /translation="MNPVYEKLQKCYESYKAKIDFTPKVALVLGSGLGDYADDIRVVD
                     TLDYHDIEGFPVSTVPGHKGRFIFGYVGEVPVVCMQGRVHYYEGYDMSDVVLPARLMK
                     LMGAEVLFLTNAAGGIQLGMNAGDFMLIKDQIASFVPSPLRGANVDELGVRFPDMSQV
                     YDKELQLLVKKAALRLDIDLKEGTYLQLSGPQFETPKEVAMCRTLGADAVGMSTACEA
                     IAARHMGMRVVGISCISNLACGISAVPLCHEEVQETADRVAPKFKALVTETIKSIGK"
     gene            1295..>2441
                     /locus_tag="ACZO9R_04850"
     CDS             1295..>2441
                     /locus_tag="ACZO9R_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR0912429.1"
                     /translation="MNTRFYNAKILILADDHKFKITEGELWVKGNTICYIGDGTDTAD
                     VCKDGEVLVWDREIDACGNLLMPGFKNAHTHTAMTFLRSYADDLPLQDWLNQQVFPRE
                     GQLTEDDVYWLNILGIMEYLTSGITSNFDMYFFPPVDAKASADTGFRTVQTSGLNNFG
                     GTVELMEENYHIVNEMSDLTSFIVGFHAEYTTSKELMEGVAALAQKLKSPVWLHNSET
                     KLEVAECKERWGMTPTQLTDSLGMYEYGGGGYHCVWFEEPDFEIFKKRHLTAVTNPAS
                     NLKLASGICPTKRFVDEGIAMAIGTDGPASNNCLDMFREMFLTSALAKVREMDAECVG
                     ADEILYMATAGGAHAMQLEDCDRLAVGKKADIVMIDLNQPNMQPENNI"
     assembly_gap    2442..2451
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2452..2596
                     /locus_tag="ACZO9R_04855"
     CDS             <2452..2596
                     /locus_tag="ACZO9R_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR0912430.1"
                     /translation="LVYSGSKQNVKMTMINGKVLYEDNHFEIGFDPKEIYQRANAIIG
                     RMK"
     gene            2653..3408
                     /locus_tag="ACZO9R_04860"
     CDS             2653..3408
                     /locus_tag="ACZO9R_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0912431.1"
                     /translation="MQVIFIHHSCFLVEVDEKVLIFDWFDGDRVEGYHFGGVLPEYEP
                     DTPVYVFASHKHRDHFDMDVLHLAEKYPKIRFIFSKDCKMSPHFLVKHGFAPEVAKRI
                     HYVGADEKCRVDDLNIETLRSTDAGVAFYVETNGAAFYHAGDLNDWHWDGAGDLINGS
                     MRTNYRSQIRRLSGRKIHLAFVPADPRLMEHQFDGMNYFLEHTDADYVFPMHMWQDYS
                     ALPDYRKRISNAAMAERVVEINHENQIFEILEE"
     gene            3523..3675
                     /locus_tag="ACZO9R_04865"
     CDS             3523..3675
                     /locus_tag="ACZO9R_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium nitrogenase"
                     /protein_id="HIR0912432.1"
                     /translation="MAFLGWFVHYVLIFVVLVVVAGLGIFAGKKLSDRKDAKNAGETG
                     NVEETK"
BASE COUNT         1019 a          705 c         1079 g          883 t
ORIGIN      
        1 tgaattgacg gaatacgata cagagcaaat tcagatagaa atttatcagt tatgggatga
       61 tgaactgaac tatttatgga aatacctgaa agaaactgta gatgaacaga caatgcaaaa
      121 acttttgctg gaacaacgag aatggattga aaaaaaggaa caaacaattc aaacgctgca
      181 gaataatgat ggagaatcgc tgacatatcc gtatcaagca gaaataaccc gaaagcgtgt
      241 atatgagctg acagaatata tacaggagta aatatgggag aaaatgtagg aactcttttt
      301 attctgaatg agattggaat ttccgtaagg atggtatata atataagtaa ttttgtgatc
      361 gaaaggaaaa ggagaactga caatgaatcc agtatatgaa aagttacaga aatgctatga
      421 aagttataag gcaaagatcg attttacccc gaaggtggca ctcgtgctgg gatccgggct
      481 gggagattat gcggatgata tccgcgtggt agacacactg gattaccacg acatcgaggg
      541 cttcccggta tcgactgttc cgggacataa gggacgtttt atcttcggat atgttggaga
      601 agttccggtg gtatgcatgc agggaagagt tcattattat gaaggatatg acatgtcgga
      661 tgttgtgctc ccggcacgtc tgatgaagct gatgggcgcg gaggtattat tcctcaccaa
      721 tgcggcaggc ggtatccagc ttggtatgaa cgccggtgat tttatgctca tcaaggatca
      781 gattgcaagc tttgttcctt ccccgctgcg aggagcaaat gtggacgagc ttggtgtgcg
      841 tttcccggat atgagccagg tatacgataa ggaattacag cttctggtga aaaaagcggc
      901 gttgagactg gatattgatt taaaggaagg cacttattta cagctttccg gtccgcagtt
      961 tgagacaccg aaggaagtgg cgatgtgccg gacactcgga gctgatgccg ttggtatgag
     1021 tacggcatgc gaggcgatcg cagcgcgtca tatgggcatg cgtgttgtgg gtatttcctg
     1081 catttccaat ctggcatgcg gtatctcggc ggtcccgctc tgtcatgagg aagtacagga
     1141 gacggcagac cgcgtggcac cgaagttcaa ggcacttgtg acggagacga tcaagagtat
     1201 cgggaaataa taaccgaaga gacgcggtga agattctgga cgggagagag gcttcataga
     1261 gtggaaacag cagacagaag aaggagaaaa tgacatgaat acacgttttt ataatgcaaa
     1321 gatcttaatc cttgcagatg accacaagtt caagatcacg gagggagaac tctgggtcaa
     1381 ggggaatacg atctgctaca tcggtgatgg gacggacacg gcggatgtgt gcaaggatgg
     1441 agaagtgctg gtgtgggatc gcgagatcga tgcatgcggc aatctgttaa tgccaggatt
     1501 taagaacgcg cacacacaca cagccatgac attcttacgt tcctacgcgg atgatctgcc
     1561 gctgcaggac tggctgaacc agcaggtgtt cccacgcgag ggacagctta cggaggatga
     1621 tgtgtactgg ctgaatattc ttggtatcat ggaatatctg accagcggca ttacgtccaa
     1681 ctttgatatg tatttcttcc cgccggtcga tgcaaaagca tccgcagata cgggattccg
     1741 tacggtgcag accagcggac tgaacaattt cggcggtaca gtagagctga tggaggaaaa
     1801 ttatcatatc gtcaatgaga tgagtgatct gacttctttc attgtcggat tccatgcgga
     1861 gtacaccacc tcgaaagaac tgatggaagg cgtggcggcg ctggcacaga agttaaaatc
     1921 tccggtatgg ttgcacaatt ccgagacaaa gcttgaggtg gcagagtgca aagagcgctg
     1981 gggcatgaca ccgacacagc ttacggactc actcggcatg tatgagtacg gcggcggcgg
     2041 ataccattgt gtatggtttg aggagccgga ttttgagatc tttaaaaagc gtcatctgac
     2101 ggcagtgacg aacccggcct ctaacctgaa gcttgcttcc ggcatctgcc cgacgaagcg
     2161 gtttgtggat gagggcattg ccatggcgat cggtacggac ggcccggcga gcaacaactg
     2221 tctggatatg ttccgcgaga tgttccttac ctccgcactt gcaaaggtgc gcgagatgga
     2281 tgccgagtgc gtgggtgcag atgagatcct ttacatggca acagccggcg gtgcgcatgc
     2341 aatgcagctg gaggactgtg accgtctggc ggtgggcaaa aaggcggata ttgtcatgat
     2401 tgacctgaac cagccgaaca tgcagccgga aaacaatatc gnnnnnnnnn ntctggtata
     2461 cagcggcagc aagcagaatg tcaagatgac gatgattaac ggcaaagttc tctatgaaga
     2521 caatcatttc gagatcgggt ttgatccgaa agagatctat cagcgtgcga atgcgatcat
     2581 cggccgcatg aaatagaaat cattagattt gcagggcaga acttctgaca ttgcggagaa
     2641 acgaggagaa ttatgcaggt tatttttatt catcacagtt gttttctggt cgaggtggat
     2701 gagaaggtgt tgatttttga ctggtttgat ggagaccggg tggaggggta tcactttggc
     2761 ggtgtgttgc cggagtatga gccggatacg cccgtctacg tgttcgcaag ccacaagcat
     2821 cgggatcatt ttgatatgga tgtgttgcat ctcgcagaaa aatatccgaa gatccggttt
     2881 attttttcaa aagactgtaa gatgagcccg cactttctgg taaaacatgg atttgcgccg
     2941 gaggtggcga agcggattca ctatgtgggg gcggatgaaa aatgcagggt cgatgacttg
     3001 aatattgaga ctctgcgctc gacggatgcc ggtgtggcgt tttacgtgga gacgaacggc
     3061 gcggcatttt accatgccgg cgatctgaat gactggcact gggacggagc cggcgatctg
     3121 atcaacggat ccatgcgtac aaattaccgc agccagatcc gcagactttc tggcaggaag
     3181 attcatctgg cgtttgtgcc ggcagatccg aggctgatgg agcaccagtt tgacggcatg
     3241 aattatttcc tggagcacac ggatgcggat tacgtatttc cgatgcatat gtggcaggat
     3301 tacagcgcgc ttccggatta tcggaagaga atctcgaacg ctgcgatggc ggagcgggtt
     3361 gtggaaataa atcatgaaaa ccagattttt gagatccttg aggaatgaga ggaatttcaa
     3421 aaaagcggta cttgacgatc ataaaacggt tgcgctatac tgatttagtt atagaagatt
     3481 tcacacgggg tgtgaagaat tggggaaagg caggacttga ctatggcatt tttgggatgg
     3541 tttgtacatt atgtactgat tttcgtggtt ttggtcgtgg tggcgggact tggaattttt
     3601 gcgggaaaga agttgagcga ccgcaaggat gctaagaatg ccggtgagac cggaaacgtc
     3661 gaagagacaa aatagattta aaatagaaag aaacat
//
LOCUS       DBMDQJ010000180         3682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9221_length_3682_cov_1.290701, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000180 DBMDQJ010000000
VERSION     DBMDQJ010000180.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9221_length_3682_cov_1.290701"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(120..953)
                     /locus_tag="ACZO9R_04870"
     CDS             complement(120..953)
                     /locus_tag="ACZO9R_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912433.1"
                     /translation="MAGKKKDTSLLHHLVGSRSLVWNLAKNDIKKKFAGSYFGVVWAF
                     VQPVITVLLYWFVFEKGLNSKATDLRTGIEIPFVLWLTAGLVPWFYFQEALNGGTGVL
                     VEYSYLVKKVVFQIDMLPVVKMISALFTHLFFVAFTMVLFICMGYMPDWYALQVIYYS
                     FCMILFATGLVYATSAVTVFFRDMKEVVGILLQIGMWVTPIMWNFETMVQIPGWAITV
                     LKLNPMYYIVSGYRNALINHIGFWEDMGLTVYFWVVTAVILFLGTTVFKRLRPHFADV
                     L"
     gene            complement(1003..1584)
                     /gene="rfbC"
                     /locus_tag="ACZO9R_04875"
     CDS             complement(1003..1584)
                     /gene="rfbC"
                     /locus_tag="ACZO9R_04875"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR0912434.1"
                     /translation="MGKIKVTENCNGIEGLKVIEPAVFGDARGYFMETYNYNDFAEAG
                     IDCQFVQDNQSASKKGVLRGLHFQINYPQDKLVRVVNGEVFDVAVDLREGSKTFGKWY
                     GVRLSAENKKQFFVPKNFAHGFIVLSEEAEFCYKVTDFYHPNDEGGILWNDAEVGVEW
                     PMPEGMTVADLILSDKDKVQKSFAEYKAAHGIK"
     gene            complement(1588..2466)
                     /gene="rfbA"
                     /locus_tag="ACZO9R_04880"
     CDS             complement(1588..2466)
                     /gene="rfbA"
                     /locus_tag="ACZO9R_04880"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR0912435.1"
                     /translation="MKGIILAGGSGTRLYPLTKAISKQIMPVYDKPMIYYPLSTLMLA
                     GIREVLIISTPRDLPVFEELFGDGSQLGMNFSYAVQEQPRGLADAFIIGEKFIGNDAV
                     ALVLGDNIFYGQSFSKVLKSAAERTEKEHGATIFGYYVRDPREYGVVEFDENGKALSI
                     EEKPEHPKSNYAVPGLYFYDNDVVEIAKNVKPSARGEIEITSVNNAYLGRGDLHVETL
                     GRGFAWLDTGNHDMLLAAADFVSAFQKRQGLYISCIEEIAYKRGFIDRDQLIKLAQPL
                     LKTDYGKYLMEIAEGL"
     gene            complement(2600..>3682)
                     /gene="rfbB"
                     /locus_tag="ACZO9R_04885"
     CDS             complement(2600..>3682)
                     /gene="rfbB"
                     /locus_tag="ACZO9R_04885"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR0912436.1"
                     /translation="MRTYLVTGGAGFIGSNYIHYMFRKYGDTIRIINVDVLTYAGNLE
                     NLKDIENNPNYTFIRADITDKEAIAKIFAENDIDRVVHFAAESHVDRSIKNPEVFVKT
                     NVLGTAVMLNCAKAAWELPDGTFKADKKFLHVSTDEVYGSLEDDGSYFYETTPYAPHS
                     PYSASKASSDMLVKAYIDTYKFPANITNCSNNYGPYQFPEKLIPLIINNALQGKKLPV
                     YGDGKNVRDWLYVDDHAKGIDMVQEKGRLGETYNIGGHNEKQNIQIIEIIIDTLQEML
                     PADDPRRKLVSKDLITYVADRKGHDRRYAIAPDKIKAEVGWEPETKFEDGIRKTIAWF
                     FEHEDWMKNVTSGDYQKYYEDMYQNR"
BASE COUNT          877 a         1077 c          739 g          989 t
ORIGIN      
        1 tttcccgcaa gtcccagcgc gtcaaacagc cgttctgagg gtctgttgta tagccggtat
       61 accttgctga catcttctac cctgattgct aactcatcca ttcagttaca aattcctctc
      121 tataatacat ccgcaaaatg cggtcgcagc ctcttgaaca cggttgttcc gagaaacagt
      181 atcactgccg tcacaaccca gaaatatacg gtaagtccca tatcctccca gaatccgatg
      241 tgattgatca gcgcgttccg gtatcccgaa acaatgtagt acatcggatt gagtttcaac
      301 acggtaatcg cccatcccgg tatctgcacc atcgtctcaa agttccacat aatcggtgtc
      361 acccacatgc cgatctgcaa gaggattccc acaacttctt tcatgtcgcg gaagaatacc
      421 gtcaccgcac tcgtcgcata cacaagccct gtcgcaaaca gaatcataca gaacgaataa
      481 tatataacct gcagcgcata ccagtccggc atatatccca tacagatgaa aagcaccatc
      541 gtaaacgcca caaaaaacag atgcgtaaac agcgccgata tcatttttac caccgggagc
      601 atatcgatct ggaacacgac tttttttacc agatagctgt attccaccag aacaccggtg
      661 ccgccgttta gcgcttcctg aaaatagaac cacggcacaa gccccgccgt cagccacagc
      721 acaaacggaa tctcaattcc ggtccgaaga tctgtcgcct tggaattcag tcccttctca
      781 aacacgaacc agtacaaaag caccgtgatt accggctgga cgaacgccca gaccacacca
      841 aagtaggagc ctgcaaactt cttcttgatg tcattttttg ccagattcca taccaggctt
      901 ctacttccga ccagatgatg aagcagtgat gtatcctttt tctttcctgc catctatgtt
      961 ctcctaatct ctttctatcc ctctgccgac gcatgttttt ccttacttta tgccatgcgc
     1021 tgccttatat tctgcgaagc ttttctgcac tttatccttg tcagataaga tcagatccgc
     1081 cactgtcatt ccttccggca tcggccattc cacgccgacc tcggcatcgt tccacagaat
     1141 accgccctca tcgttcggat ggtagaagtc cgttaccttg tagcagaact ctgcctcctc
     1201 cgacagcacg ataaatccgt gtgcaaaatt cttcggcacg aaaaactgct tcttgttctc
     1261 cgcggaaagc cgcacgccgt accatttacc gaacgtcttc gatccctctc ttaaatccac
     1321 cgcaacgtcg aacacctcgc cgtttaccac gcgtaccagc ttgtcctgtg gataattgat
     1381 ctgaaaatga agcccacgga gcacgccctt tttcgatgcc gactggttgt cctgcacgaa
     1441 ctggcagtca attccagctt ccgcaaaatc attataattg taggtctcca taaaatatcc
     1501 gcgcgcatcg ccgaatactg ccggctcgat gaccttcaat ccctcaatgc cgttgcaatt
     1561 ctctgttacc ttgatcttac ccatgttcta taatccctcc gcaatttcca tcagatattt
     1621 gccgtaatct gtcttcaaga gtggctgtgc cagcttgatg agctggtctc tgtcgatgaa
     1681 gccgcgcttg tacgcgatct cctcaatgca ggagatatat aagccctggc gtttctggaa
     1741 tgcagacaca aaatctgccg cggcaagcag catgtcgtgg tttcccgtat ccagccatgc
     1801 gaatcctctt cccagtgtct cgacatgcag atcccctctg ccaagatatg cattattgac
     1861 ggatgtgatc tcgatctccc cacgtgcgga cggcttgacg tttttcgcaa tctccaccac
     1921 gtcgttatcg taaaagtaaa gtcccggcac cgcgtaattg gacttcggat gctccggctt
     1981 ttcctcgatg gacagcgcct tgccgttctc atcgaattcc acgacgccgt actctctcgg
     2041 gtctctgaca taatagccga agatcgtcgc accgtgctcc ttttccgtgc gctccgccgc
     2101 actctttaac actttggaaa agctctgtcc gtagaaaata ttatcgccga gtaccagtgc
     2161 caccgcgtca tttccgataa atttttcacc gataataaat gcgtccgcca gtcctctcgg
     2221 ctgctcctgc accgcatagg aaaagttcat gccgagctgg cttccatccc cgaaaagttc
     2281 ctcaaacacc ggcagatccc gcggtgtgga gatgattaac acctcacgga ttcccgcaag
     2341 catcagtgtc gataacggat agtagatcat cggcttgtca tacaccggca taatctgttt
     2401 tgaaatcgct ttggtaagcg gataaagtct ggtaccggag cctccggcaa gtattattcc
     2461 cttcatctga aaatcctccc attttatcca atatacgcca tgtaccttaa catttgtacc
     2521 ggacacagct taattttttc ttaatttaaa acgcaaacaa gcagtatcgc ccgaaagacg
     2581 gtactgctgc ctgcatcaac tatctgttct gatacatatc ctcataatat ttctgataat
     2641 cgccgctggt gacattcttc atccagtcct cgtgctcgaa gaaccacgcg atcgtcttgc
     2701 ggatgccgtc ctcaaacttt gtctccggct cccagcctac ctccgccttg atcttgtccg
     2761 gcgcaatcgc atagcgtctg tcatgtccct tgcggtctgc cacgtaagta atcagatcct
     2821 tgcttaccag ctttcttctc ggatcgtctg ccggaagcat ctcctgcagc gtatcaataa
     2881 taatctcaat gatctggata ttctgttttt cgttatgtcc gccgatgttg taagtctcac
     2941 ccagacgtcc cttctcctgc accatatcga tgcccttcgc atgatcgtcg acatacagcc
     3001 agtcacgcac attcttgccg tcgccgtaca ccggaagctt cttgccctgc agcgcattgt
     3061 tgataatcag cgggatcagc ttctccggga actgatacgg tccgtaattg ttggaacagt
     3121 tcgtgatgtt cgccgggaac ttgtaggtat caatgtatgc ctttaccaac atatctgagg
     3181 atgccttgct ggctgaatac gggctgtgtg gtgcgtacgg agtcgtctca taaaaataag
     3241 atccgtcatc ctcgagggag ccgtatacct catccgtcga tacgtgtaaa aacttcttgt
     3301 ccgccttaaa tgtgccgtcc ggaagttccc atgctgcttt ggcacagttc aacatcactg
     3361 ccgttccgag cacgttcgtc ttcacgaaga cctccggatt cttgatactg cggtctacat
     3421 ggctctctgc agcaaagtga accacacggt caatgtcgtt ctccgcgaaa atcttcgcaa
     3481 tggcttcctt atccgtaatg tccgcccgga taaacgtata atttggattg ttctcaatgt
     3541 ccttcaggtt ctcaaggttt cccgcatacg tgaggacatc cacattaatg atgcggatcg
     3601 tatctccgta tttgcggaac atgtagtgaa tgtaattgga accgataaaa ccggctcccc
     3661 ctgttaccaa ataagttctc at
//
LOCUS       DBMDQJ010000181         3678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9240_length_3678_cov_1.526419, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000181 DBMDQJ010000000
VERSION     DBMDQJ010000181.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9240_length_3678_cov_1.526419"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="ACZO9R_04890"
     CDS             complement(<1..513)
                     /locus_tag="ACZO9R_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016298167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR0912437.1"
                     /translation="MRTVVLLYDTSCIYEIVILNYFLKFTGKDVTFVSMDGNLITSME
                     GYSINVSGKLTDIPVKDIELIIVPGGNIKEIDTDAVWNCLRNIQMNQGIIAGICAGVD
                     VLDHASILDGIASTHSTDLNVVSADRVITSRANGYVDFAIEVAKEMHLFKDEADLEET
                     IAFWRNHQRME"
     gene            complement(520..2121)
                     /locus_tag="ACZO9R_04895"
     CDS             complement(520..2121)
                     /locus_tag="ACZO9R_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR0912438.1"
                     /translation="MSDQTVLIDQFLSSVISTIHPDEIFDSIIDTAVRLLSPDGLLLT
                     IRDDVSPSYHVTRSFGDITWEKDSFLDSSILLEQLLNADSYIPFMPGAGLPAALDGIS
                     SHTRSWLIQQNIIGVYCLKYNRALIGFLFIARHNHMPLSADQVSALCKIGFYATHALR
                     NANLYMNAYRASITDDLTALYNRKHAFEIIDELCLSGTPCSLIMLDIDDFKLYNELYG
                     SKEGDHLIQRCAKIILSALDADDMAFRFSADEFLILRHGSDVAASKAFTQSLVEVLTK
                     GSMSDMVWEITITCGISVFPDISVDGRELIHNVEQAVYFGKLDGKGHLIVYRKGLENR
                     SQNPDIRTAYERVAPTIYALTAAIDAKDNFTFIHSMNVSKYAVMLAEALPMSDNDIEV
                     VRIAGMLHDIGKISIPEHILKKTSGLTPEEYTIMKTHVENSIKMIRYLPNMDYVIPAV
                     VAHHERYDGTGYPRGLKGTEIPLMGRILTIADCFDAMTARRPYKQECSIDYAAGELKK
                     NSGTQFDPELVPVFLSLIEDGKIVG"
     gene            2244..2474
                     /locus_tag="ACZO9R_04900"
     CDS             2244..2474
                     /locus_tag="ACZO9R_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912439.1"
                     /translation="MRREEFKMERTGLLEIGDVLPITEGKLPNSYYYTLGKAYAMSAN
                     YVVSQRIKSREGTVVDVKETPKGYFVTVEFDE"
     gene            2516..3574
                     /locus_tag="ACZO9R_04905"
     CDS             2516..3574
                     /locus_tag="ACZO9R_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR0912440.1"
                     /translation="MRYLPTSRLTPGMALGQDIYDGAGRLLLGRHILLTEEQISNLEF
                     LGFPGVYVDDEFSRGIEIQQIVSPEVRRQALKIVYDLFHTDMRKQEPSEAEFKLRKTV
                     ESVVDDVICNGDIMCNIMDIKSYDDYIYYHSIHVGILSVVVGARLGLPHDELCQLAAA
                     ALLHDIGKRFIDHDIVRGGKAHRSEKEQEVYRSHPKIGAEYLRETCRFSADVYEGIME
                     HHECYNGEGYPLGKKGGEIHLFARIIRIADCYDAKVSAFPAQKSLSPSEALEYVMALG
                     DRWFDPDIVAVFARKVAAYPIGCEVELSNGQHAVVARNFENFPLRPLVRVVETGEMLN
                     MRDDEKARAVTVGRLIVR"
BASE COUNT          952 a          806 c         1018 g          902 t
ORIGIN      
        1 ctccatcctc tggtgattcc gccagaatgc gattgtttcc tccaggtcgg cttcatcctt
       61 aaataaatgc atttccttag ccacttcaat ggcgaaatct acatagccat ttgcacggga
      121 agtgatcacc cggtctgctg aaacgacatt aagatcggta gagtgcgtgg atgcaattcc
      181 atctaaaatg ctggcatgat ctagtacgtc tactccggca cagatcccgg caatgatccc
      241 ctgattcatt tgaatatttc tgaggcagtt ccacacagca tcagtatcaa tttccttgat
      301 attcccaccg ggtactatga taagttcgat gtcttttacc ggaatatctg tcagcttgcc
      361 ggaaacattg attgagtaac cttccataga agtgatgaga tttccgtcca tcgatacaaa
      421 tgtgacatct tttccggtaa attttagaaa ataatttaat atcacaatct cgtaaatgca
      481 gcttgtgtca tataaaagta caactgttct cataaatttc taccccacaa tctttccgtc
      541 ttcaataaga gacaagaaca ccggcaccag ttcgggatcg aactgcgtgc cgctgttctt
      601 ttttagttca cctgccgcgt agtcgatcga gcattcctgc ttgtaggggc gtctggccgt
      661 catcgcgtca aagcagtctg caatggtaag gatacgtccc atgaggggaa tttccgttcc
      721 ttttaatccg cggggataac cggttccgtc atagcgctcg tggtgggcga caactgccgg
      781 gatcacgtaa tccatgttcg gaaggtaacg gatcatctta atggaattct ccacatgcgt
      841 tttcatgatc gtgtattcct ccggcgtcag ccccgaggtt ttctttaaga tatgctcagg
      901 gatgctgatc ttgccgatgt cgtgaagcat gcctgcaatc cgtaccacct cgatatcatt
      961 gtcgctcata ggaagcgctt ctgcgagcat gaccgcatat tttgagacat tcatggaatg
     1021 gatgaacgta aaattatcct tggcgtcgat ggcggcggtc agcgcataga tggtcggcgc
     1081 cacccgctcg taggcggtgc ggatatccgg attctgggag cggttctcaa gacctttgcg
     1141 gtaaacgatg agatggcctt ttccgtcaag ctttccaaaa tagacagcct gctcgacatt
     1201 gtggattaac tctctgccgt cgacagaaat atccggaaac acggagatgc cgcaggtgat
     1261 ggtaatttcc cacaccatgt ctgacatgct gccttttgtc agaacctcca cgagagactg
     1321 ggtaaacgct ttggaggctg cgacgtcact gccgtgtctt aagatgagaa attcgtccgc
     1381 actgaaacga aatgccatgt cgtccgcatc cagagcagag agaatgatct tggcacagcg
     1441 ctggattaag tgatcgccct ccttggagcc gtaaagttca ttgtaaagct taaaatcatc
     1501 gatatcgagc atgattaatg agcacggcgt accggagagg cagagttcat caattatctc
     1561 aaacgcatgc ttgcggttgt agagtgccgt cagatcatcc gtgatggagg ctcggtaggc
     1621 attcatgtag agattcgcgt tgcgcagtgc gtgggtggca taaaaaccga tcttgcacag
     1681 cgcggacacc tgatctgcgg acagaggcat gtgattgtgc cgcgcgatga agagaaagcc
     1741 aatcagcgcc cggttgtact tcagacaata gacgccgatg atattctgct ggatcagcca
     1801 gctgcgcgta tggctggaaa tgccgtcaag cgcagctggc aggcctgcgc ccggcataaa
     1861 cggaatgtag ctgtctgcgt tcaaaagctg ttccaaaaga atgcttgaat cgagaaaaga
     1921 atctttttcc caggtgatat cgccaaaact gcgtgtgacg tggtaggaag gactgacatc
     1981 gtcacggatc gtgagcagca gaccgtccgg agacagcaga cggacggctg tgtctataat
     2041 agaatcaaag atttcgtccg ggtgaatcgt gctgatgacg gatgacagga attgatcgat
     2101 gagaacagtt tgatcgctca taaggctcct ttgtatatga taaatgaaaa catcttgaga
     2161 attcagaatc attatactat aatgaaaaat gcatggcaat agtgggaaat acatagaaaa
     2221 gccatagaaa cagggatatt ttcatgcgta gagaagagtt taaaatggag cgcaccggac
     2281 tgcttgagat cggcgatgtg cttccgatta cagaggggaa gctgcccaat tcctattact
     2341 atacactggg caaggcgtac gcgatgtcgg cgaactatgt ggtaagccag cgcatcaagt
     2401 cgcgggaagg cacagtggta gacgtcaaag agaccccgaa gggatatttt gttacggttg
     2461 aatttgatga gtaaaagcgc taaaataata ataatttagc ggaaaggaag cacaaatgcg
     2521 ttatttaccg acgagcagac tgacgccggg aatggcactg gggcaggaca tttatgacgg
     2581 ggcgggaaga cttcttctgg gaaggcatat tctgctgaca gaagaacaaa tttcaaatct
     2641 ggaattcctt gggttcccgg gtgtctatgt ggacgatgag ttttccaggg ggattgagat
     2701 acagcagatt gtcagcccgg aagtccggag gcaggcactg aaaattgtat atgatctgtt
     2761 tcacacggat atgcggaagc aggaaccgtc ggaagcggag tttaagctgc gcaaaacggt
     2821 ggagtctgtg gtcgatgatg tgatatgcaa tggagacatc atgtgcaata tcatggacat
     2881 caagagttat gatgattata tctattacca ttccattcat gtgggcatcc tttctgtggt
     2941 agtcggcgca cggctggggc ttccgcatga tgagctgtgc cagcttgcag cggcggcgct
     3001 gctgcatgac attgggaagc ggtttatcga tcatgacatc gtgcggggag gtaaggcgca
     3061 ccgcagcgaa aaagagcagg aggtctatcg gagccatccg aagatcggtg cggaatatct
     3121 gcgcgagaca tgccgatttt ccgcggatgt gtatgaggga atcatggagc atcacgagtg
     3181 ctacaacggc gaagggtatc cgcttggcaa aaagggcggc gagattcacc tgtttgcccg
     3241 cattatccgg attgcagact gttatgacgc aaaggtttcc gcgtttccgg cacagaaatc
     3301 actgtcgccg tcggaggcgt tagaatatgt gatggcgctg ggcgaccgct ggtttgaccc
     3361 ggatattgtg gctgtgttcg caaggaaagt ggcggcgtat ccgatcggct gcgaagtcga
     3421 actgtccaac gggcagcatg cggtggtggc acggaatttt gagaatttcc cgctgcgtcc
     3481 gctggtgcgc gttgtggaga cgggagagat gttgaacatg cgtgacgatg aaaaagcgag
     3541 ggcggtaact gttgggagac tcatagtgcg atagagcacc tggggcgaga gggagttatg
     3601 actggacagg gaaagagtgc gattgataca cttttggcag aaagctttaa ggaactggcg
     3661 ttaaagcagc cgatagag
//
LOCUS       DBMDQJ010000182         3669 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9266_length_3669_cov_1.565583, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000182 DBMDQJ010000000
VERSION     DBMDQJ010000182.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9266_length_3669_cov_1.565583"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..91
                     /locus_tag="ACZO9R_04910"
     CDS             <1..91
                     /locus_tag="ACZO9R_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912441.1"
                     /translation="KTEIKDSLGDFVWKETKRRPMIMPIIMEV"
     gene            98..592
                     /locus_tag="ACZO9R_04915"
     CDS             98..592
                     /locus_tag="ACZO9R_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR0912442.1"
                     /translation="MKWNKVVFKFVSISFSILVALLVVVGLIELGSYCYDFGYRVFTE
                     SPVDEAPGRDVTVSVTSDMSEHDIGKMLEEEGLVEDANLFYAQLKLSAYSGKLKPGVY
                     ALNTSMTAREMFVIMAADTDDTESAEDTENTADNGNTGAADLTDETDDTQTAEDAGDA
                     EETP"
     gene            589..1230
                     /locus_tag="ACZO9R_04920"
     CDS             589..1230
                     /locus_tag="ACZO9R_04920"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR0912443.1"
                     /translation="MIVDERLVTYINSLDTGNTAMLDQIEREATADYVPIIRKEMQSF
                     LKFLLAMKKPARILEVGTAVGFSAILMAEYDPVPCQITTIENYEKRIPIARENFKKAG
                     KEAQIALLEGDAAEVLKTLEGPYDFIFMDAAKGQYIHFLPEILRLLAKDGVLVSDNVL
                     QDGDVIESRFAVTRRNRTIHKRMREYLYTLTHSEELVTAVLPVGDGITLSTRR"
     gene            1336..2559
                     /locus_tag="ACZO9R_04925"
     CDS             1336..2559
                     /locus_tag="ACZO9R_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR0912444.1"
                     /translation="MRRPELLIPASSLEVLKIAVIYGADAVYIGGEAFGLRAKAKNFS
                     MEEIREGIAFAHAHDVKVYITANILAHNGDLDGVREYFAELREIKPDALIISDPGVYM
                     IAKEVCPEIERHISTQANNTNYGTYRFWYEQGAKRVVSARELSLAEIKEIRANIPDDL
                     EIETFIHGAMCISYSGRCLLSNYFTGRDANQGACTHPCRWKYAVVEEKRPGEYLPVYE
                     NERGTYIFNSKDLCMIEHIPELMESGIDSFKIEGRMKTALYVATVARTYRKAIDDYKQ
                     SPELYREHMAWYQEQISNCTYRQFTTGFFFGKPSDEAQIYDNNTYVKEYTYLGIVGER
                     NEEGLYRVEQRNKFSVGESIEVMKPDGANITVTVQRIVDEEGHDMESAPHPKQVLYID
                     LGQPLAMYDILRRKE"
     gene            2574..3635
                     /locus_tag="ACZO9R_04930"
     CDS             2574..3635
                     /locus_tag="ACZO9R_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR0912445.1"
                     /translation="MKQEKYVAYVGTYTHENSVGIHIYDVDVEAGKLTERKVVPINNP
                     SDLVVSKDRRFLYSIADEGVESFRIEKDGDLTSINKQWIGGMRGCYVEVDDGNRYLFV
                     GGHHDGRVSMMHLEADGSIGGIADGIFHKGIGRSIAQRNFVPHVDCVKLTPDQKFLCA
                     VDNGLDQVKVYRVDYENGKLELIDIIRGPLESAPRMIRFSRDGEYAYILYELLNIVEV
                     YHYSVQDNEPVFEKIQSISTSPKKEEDTCAASGMEISKSGKYLFCSNAGVNSVVCYEI
                     EEKTGMLTPVFTTKVNSDYPKMLAIYPDEKHYLTLNNNSNDIFSYTIDYKDGYSLETG
                     SPVKVDKPNCIYMLKLEVE"
BASE COUNT          985 a          741 c         1105 g          838 t
ORIGIN      
        1 caagacggag atcaaggatt ctctgggaga ttttgtgtgg aaagagacga agagacgccc
       61 gatgattatg ccgataatta tggaagtgta gaagattatg aaatggaaca aagtcgtttt
      121 caaatttgtc agtatcagct tttccatcct ggtcgcactg cttgttgtgg tgggattgat
      181 tgaacttggc tcctactgct atgacttcgg ataccgggtg tttaccgaga gcccggtgga
      241 cgaagcgccg ggcagggatg tgaccgtatc cgtgacttcg gatatgtcgg agcatgatat
      301 cggaaagatg ttagaagaag agggactggt ggaggatgcg aatctgttct atgcccagct
      361 gaagctatct gcttattccg gaaaactcaa accgggtgtt tacgcgttaa atacctcgat
      421 gactgcgcgg gagatgttcg tgatcatggc agcggatacc gatgatacgg aaagtgccga
      481 ggacaccgag aataccgcag acaacgggaa taccggggcg gcggatctga cagatgagac
      541 ggacgataca cagaccgcag aagatgcagg ggatgcagag gagacaccgt gatagtagat
      601 gagagacttg tgacctatat caattctctg gatacgggaa atactgcgat gctcgatcag
      661 attgagcggg aagcgacagc cgattacgtg ccgattattc ggaaggagat gcagagtttt
      721 ttgaagtttc tgcttgccat gaagaaaccg gcacgtattt tagaagttgg gactgcggtg
      781 ggattttccg ccatcctcat ggcggagtac gatcccgtgc cgtgtcagat tacaacgatc
      841 gagaattacg aaaagcggat tccgattgcc agggagaact ttaagaaagc cgggaaggaa
      901 gcgcagattg cgctgctgga gggcgatgcc gcggaagtgt taaagacgct ggaaggtccg
      961 tatgatttta tttttatgga cgcggcaaaa gggcagtaca ttcatttcct gccggagatt
     1021 ctgcgccttc tggcgaagga cggcgtgctg gtgtcggaca atgtgctgca ggacggcgat
     1081 gtgatcgaat caaggttcgc ggttacaagg cggaaccgca cgattcataa gcgcatgcgg
     1141 gaatatctgt acacgctgac gcacagtgaa gaactggtga ccgcggtgct cccggtggga
     1201 gatggcatca ccttgagcac aaggcgttaa ggacggcatg gattacgcag gatgggaaga
     1261 tggcggaatc cgtgcgggat tacatgaagc atgaagatgc gatgcatgcg tgtgagaata
     1321 gagaggaacg agaatatgag aagaccggag ttactgatac cggcgagcag tctggaagta
     1381 ttaaaaattg ctgtgatcta cggagcggat gcagtatata tcgggggcga agcgttcgga
     1441 ctccgcgcga aagcgaagaa cttttccatg gaagagatca gggaggggat tgcttttgcg
     1501 catgcccatg atgtgaaagt atacattacc gccaatatcc tggcgcacaa cggagatctc
     1561 gacggtgtga gagagtattt tgccgagctt cgggagataa agccggatgc gctgatcatt
     1621 tccgatccgg gggtctatat gattgctaag gaagtctgtc ccgagatcga gcgccatatc
     1681 agtacgcagg caaacaacac caattacggt acctaccggt tctggtatga gcagggggca
     1741 aagcgcgtgg tgtcggcgag agagctttcg ctggctgaga tcaaggaaat ccgtgccaat
     1801 attccggacg atcttgagat cgagaccttt attcacgggg caatgtgcat ctcctactcg
     1861 ggaaggtgct tactgtccaa ctattttacc ggaagggatg ccaaccaggg agcgtgcaca
     1921 catccgtgcc gctggaaata cgcggttgtg gaggaaaaac gcccgggaga atacctgccg
     1981 gtctatgaga atgagcgcgg tacttatatt ttcaattcca aggatctttg catgatcgaa
     2041 catattccgg aattgatgga gtcgggtatt gacagtttta aaattgaggg tagaatgaag
     2101 acagcactgt atgttgccac ggtggcgaga acatatcgaa aggccattga tgactacaag
     2161 cagtcgccgg agctttacag ggagcatatg gcgtggtatc aggagcagat ttcaaactgc
     2221 acctaccggc agtttacgac tggatttttc tttggcaaac cttcggacga agcgcagatt
     2281 tacgacaata acacctatgt gaaggagtac acgtatctgg gcattgtcgg agagcggaac
     2341 gaagagggac tctaccgggt ggaacagcgc aataagtttt ctgtcgggga gagcattgag
     2401 gtcatgaagc cggacggtgc caatatcacg gtgactgtgc agcgaatcgt tgatgaagag
     2461 gggcatgata tggagtccgc gccgcatccg aagcaggtgc tttatatcga ccttggacag
     2521 ccgcttgcca tgtatgacat tttgcgaaga aaagaatagg atggtggaag acgatgaagc
     2581 aggagaagta tgttgcatat gtaggaactt atacacacga gaacagcgta ggaatccata
     2641 tctatgatgt agacgttgag gcgggaaaac tgacggagcg caaggtggtt ccgatcaaca
     2701 atccgtcgga tctggttgtc tccaaggatc gcagatttct gtattccatt gcggatgagg
     2761 gcgtagaatc cttccgtatc gagaaggacg gtgatctcac ttcgatcaac aagcagtgga
     2821 tcggcggaat gagaggctgc tatgtggaag tggatgacgg taaccgctat ctttttgtgg
     2881 gtggacatca tgatggtcgt gtgagcatga tgcatctgga agcggacgga agcatcggtg
     2941 gaatcgcgga cggcattttc cacaaaggaa tcggaagaag tattgcacag cgcaatttcg
     3001 taccgcacgt ggactgtgtg aagctgacgc cggatcagaa gtttctgtgt gcggttgaca
     3061 atggactgga tcaggtaaag gtctaccgcg tggattatga gaacggcaag ctggagctga
     3121 ttgacattat ccgtggaccg ctcgagtctg ccccgcgtat gatccgtttc tcaagagacg
     3181 gcgaatatgc atatattctc tatgaactgc tcaatattgt ggaggtatat cattattctg
     3241 tgcaggataa tgaaccggtt tttgagaaga tccagtccat cagcacttcc ccgaagaagg
     3301 aggaggatac ctgtgcagca tcgggtatgg agatctcgaa gagcggtaag tatctgttct
     3361 gcagcaatgc aggcgtgaat tctgtcgtgt gctatgagat cgaggagaag acgggaatgc
     3421 tcaccccggt atttacgacg aaggtgaaca gcgattaccc gaagatgctt gccatctatc
     3481 cggatgagaa gcattatctg acactgaaca acaattccaa cgatattttc agctacacga
     3541 tcgattacaa ggacggttat tcgcttgaga ccggttcccc ggtgaaggta gacaagccga
     3601 actgcatcta tatgctgaag cttgaggtgg aataagcaaa taaagtgcga atcaggaact
     3661 gccgcaata
//
LOCUS       DBMDQJ010000183         3659 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9284_length_3659_cov_1.804103, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000183 DBMDQJ010000000
VERSION     DBMDQJ010000183.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9284_length_3659_cov_1.804103"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(87..1289)
                     /locus_tag="ACZO9R_04935"
     CDS             complement(87..1289)
                     /locus_tag="ACZO9R_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912446.1"
                     /translation="MEKKKRYLLNCEVCDARKMHENLFDPYDEVVINADVLLVNEDAR
                     RNLSRYPIQLNVDNIVEAEGDVDVQVQNGWMELKCGQMPARKTVLVVNGGLAIWPGTE
                     ELLKNYLAIIVNGSVRYPESLSAGLTRMKVNGKTSVYPDDCIILKGSAKIDAYFPLRA
                     EAGARYYAERRVVLLDEKLDVAALAEKQVRFETQELLVTESNVEAAVPLVGAATTLTV
                     VPDGCAYVDEVDGVSLDERLLRKYGKKLYINGDLSLDANSTPYLAQIEYLYVNGTVLL
                     LPEQKDAFLALNVDYKMMIVRSGLQIANRLKAFVGPDLLDETPLGITIQDCVQVILED
                     DLTAGQIQEKLKFKDCMEIICSEAQKQAVQMVSENVARINTDPGKGSKEEEDAFDGVR
                     INAESYVL"
     gene            complement(1293..1757)
                     /locus_tag="ACZO9R_04940"
                     /pseudo
     CDS             complement(1293..1757)
                     /locus_tag="ACZO9R_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019265.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
     gene            complement(1816..3204)
                     /locus_tag="ACZO9R_04945"
     CDS             complement(1816..3204)
                     /locus_tag="ACZO9R_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018594891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912447.1"
                     /translation="MKQETEEWRAAEKKQYEKMTETPVSKLIIMLGIPTTISMLVTNI
                     YNMADTYFVGELGTSASGAVGVVFGLMAIIQAFGFMFGHGAGSIISRRLGAKDVESAT
                     RFASTSFVSALAAGGLITVLGLLFLEPLMRLLGSTDTILPYAKTYAIFILIAAPFMAS
                     SCVMNNILRYEGRAALAIVGLISGSVINIFGDWFLMTRCHLGVEGAGISTAVSQLISF
                     GILFYMFSSNKTQSRLALRYVTREPKEVLMICKTGLPSLMRQGLSSVSTMMLNGQAGV
                     YGDAAVAAMSIVNRICMFVFSVGLGIGQGFQPVSAFNYGAKKYGRVRKGFYFTITAGE
                     VLLGAIAVLGMFFPAQLVAVFQNDPEVVAIGEVALRVQLVALFFQPMTVCANMMFQSI
                     GKNGRATLLAMLRSGLCFIPVLLLLSNTMGLTGVEVSQTVADILSFFISLPFALHFLR
                     ELDQREKERPKI"
     gene            complement(3385..3457)
                     /locus_tag="ACZO9R_04950"
     tRNA            complement(3385..3457)
                     /locus_tag="ACZO9R_04950"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3422..3424),aa:Lys,seq:ttt)
     gene            complement(3484..3555)
                     /locus_tag="ACZO9R_04955"
     tRNA            complement(3484..3555)
                     /locus_tag="ACZO9R_04955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3521..3523),aa:Gln,seq:ttg)
     gene            complement(3586..3659)
                     /locus_tag="ACZO9R_04960"
     tRNA            complement(3586..3659)
                     /locus_tag="ACZO9R_04960"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3623..3625),aa:His,seq:gtg)
BASE COUNT          882 a         1150 c          743 g          884 t
ORIGIN      
        1 ctgatatttt cgcactgcaa cgcgttgcag tgcgaatcca cctaagtatg atacagcttt
       61 ataccgggac tgtcaacctc ttttcactat aaaacataag attccgcgtt gatccgtact
      121 ccgtcaaatg cgtcttcttc ctctttggag ccttttcccg gatccgtatt gattctggca
      181 acattttcgc tgaccatctg taccgcctgc ttctgcgcct cgctgcagat gatttccata
      241 caatctttaa acttcaattt ttcctgaatc tgccctgccg tcagatcatc ctccagaatg
      301 acctgcacac aatcctgtat cgtaatgcca agaggcgtct catccagcag atccggtccc
      361 acgaacgctt tcaggcggtt cgcaatctgc agtccgctcc ggacgatcat catcttgtaa
      421 tccacgttca gcgccagaaa tgcgtccttc tgctccggca gaagcagtac cgtcccgttg
      481 acgtacaggt actcgatctg tgcgagatac ggtgtgctgt ttgcatccag actcagatcg
      541 ccgttaatgt aaagcttctt tccatatttg cgcagaagcc gctcatccag actcacgccg
      601 tctacctcat ccacataggc acatccatcc ggcaccacgg taagcgttgt cgcagccccc
      661 accagcggaa ccgcagcttc cacgttgctc tccgtcacca gaagctcctg cgtttcaaac
      721 cgtacctgct tttcggcgag tgccgctaca tcgagctttt catccaggag aaccacgcgg
      781 cgctccgcat aataccgtgc ccccgcttct gcccgcagcg gaaaataggc atcaatctta
      841 gcgcttccct tcaatataat acaatcatcc ggatacacac ttgtttttcc gttgaccttc
      901 attctggtaa gccccgccga aagactttcc gggtaacgca cagaaccgtt gacgataatc
      961 gcaaggtaat tttttaaaag ttcctccgtg cccggccaaa tggcaagtcc tccgttgacc
     1021 accagcactg tttttctcgc cggcatctgg ccgcatttca gttccatcca gccgttctgc
     1081 acctgcacgt ccacatcgcc ctctgcctcc acgatattat cgacattcag ctggatcgga
     1141 taccggctca gattccttct ggcatcctcg ttcaccagca gcacgtccgc attgatcaca
     1201 acctcatcgt acgggtcaaa cagattctcg tgcatctttc tcgcgtcaca tacctcacaa
     1261 ttcaacagat aacgtttctt tttctccatc ctttaatcct cccactctct ctgcaaatgt
     1321 tttctcgccg aagcaagccg catacgcacc gtcgccggcg gtattccgaa aagctccccc
     1381 agttccctgg aattgtagcc ttccagataa cgcatggtaa acaggatgcg ctcttcttcc
     1441 ggcagcccgg aaaggagctg tgcattttcc aattctccat aatccgtgta ccgtgcttcc
     1501 tccggcagtt ctgccatcac cgcctcccgg cgctcacgcc ggaaccggtc cacaaacaga
     1561 tttctgaccg tccggtagag ccaggctctt ccctgcgcaa gatccatgcc gtccagctcc
     1621 tgcgtatgat gcagtgcgcg gtaaaatgct tcctgcacca gatcctccgc ggcagattcg
     1681 ttctgtgtca tacgcctgca ccaccggatg agttccgcat gatatttttc atagagatct
     1741 tcaatcaccg cttcccctcc tctctgactg ccttcctata gataacgttt gacagcggga
     1801 aagtgttgta ttttttcaga tttttggacg ctctttttct ctctggtcca attcacgcag
     1861 aaaatgaagc gcaaacggca gcgaaatgaa gaaggataaa atatcggcga ccgtctgcga
     1921 gacctcgacg ccggtcagtc ccatggtatt tgacagcaga agcagtaccg gaataaaaca
     1981 cagtccgctc cgaagcatgg caagcagcgt tgccctgccg tttttcccga tactctgaaa
     2041 catcatgttc gcgcagaccg tcatcggctg gaagaacagc gccacaagct ggacgcggag
     2101 cgcaacctct ccgatcgcca ccacctcggg atcattctga aataccgcca caagctgcgc
     2161 cgggaaaaac atgccgagca ccgcaatcgc gccgagcagg acttcccccg ctgtgatcgt
     2221 gaaatagaag cccttcctca ccctgccgta cttttttgct ccataattga acgccgacac
     2281 cggctggaac ccctgaccga ttccaagtcc gaccgagaag acaaacatgc aaatccggtt
     2341 cacgatcgac atggccgcca ccgccgcatc tccgtacaca cccgcctgcc cgtttaacat
     2401 catcgtggac acactcgaca atccctgccg catcaggctg ggcagacctg tcttacagat
     2461 catgagcact tcttttggct ctctggtaac ataccgcagc gcgagccggc tctgcgtctt
     2521 attgcttgaa aacatataga acaggattcc aaaactgatg agctgggaca ccgccgtgga
     2581 gattcctgct ccctccaccc caagatgaca ccgcgtcatc agaaaccagt ccccgaagat
     2641 attgatgaca cttcccgaga tcagccctac tatcgcaagc gctgcccgtc cttcgtaacg
     2701 cagaatatta ttcatcacgc agctgctcgc cataaacggc gccgcaatca gaatgaagat
     2761 ggcatacgtc tttgcatacg gcagaatcgt gtccgtactt cccaacaagc gcatcagcgg
     2821 ttcgagaaac aaaagtccca ataccgtaat caatcctccg gcagccagcg cgctcacaaa
     2881 cgaggtcgac gcaaaccgcg tggcgctctc tacatccttc gcgccgagcc ttctggagat
     2941 aatacttccc gctccatgcc caaacataaa tccgaaagcc tgaataattg ccatcagtcc
     3001 aaacactacg ccgaccgctc ccgaagcact tgtcccgagc tccccgacaa aataggtgtc
     3061 cgccatatta taaatatttg tgaccagcat gctgattgtc gtcggtatgc caagcataat
     3121 aatgagcttt gacaccggcg tctccgtcat tttttcatac tgctttttct ctgccgcacg
     3181 ccattcctct gtctcctgct tcatttgttt cacatcctta tcatcattta aaagaatagt
     3241 tacctaaact atagcacaat atttcttcta caccatacac tacaggtatc catcagattc
     3301 catcctataa gaaaaaaaac gcttaacacg acgcaaaaaa agcgggaagc ctttgttttt
     3361 ttgctttccg cttatcttct tcagtgagac acgggggatt cgaacccccg acaacctgat
     3421 taaaagtcag gtgctctacc aactgagcta gtatccccta tatcagatta caacaaaagc
     3481 aagctgggct agcaggattc gaacctgcga atgctggaat caaaatccag tgccttaccg
     3541 cttggcgata gcccaacaaa taatcgttgc acctatcgca actaaggtgg atacagggat
     3601 tcgaaccctg ggcctccaga gccacaatct ggcgcgctaa ccaactgcgc tatacccac
//
LOCUS       DBMDQJ010000184         3659 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9285_length_3659_cov_1.553682, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000184 DBMDQJ010000000
VERSION     DBMDQJ010000184.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9285_length_3659_cov_1.553682"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(210..>1190)
                     /locus_tag="ACZO9R_04965"
     CDS             complement(210..>1190)
                     /locus_tag="ACZO9R_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR0912448.1"
                     /translation="EQTADAEQTDAGGQPEDAQSTDADQMSAEEQEWQNRLMADVDDF
                     LYIRESGDENAAIVGKLYKGDVAEITEAGDTWTHVESGNVDGYVKNSYCVTGSEAYTY
                     ANDTFDTEVEIQTNGLRIRSSADPDASVLAAVSTGMTFPLDADAETVDGWVAIDYKGT
                     TGYVSAEYVTTELALGEAVTIEEEQAAAAKAAAEAAKSAQVSSAGTVQNSAVAASVDD
                     VTLLAALIQCEAGNECYEGQLAVGAVVMNRVRSGRYAGSIYGVIYQAGQFPPAGRGSV
                     ASIAANGPKSSCIQAAQEAINGADNTGGATCFSRASRGHAGVVIGNHVFY"
     assembly_gap    1191..1200
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1201..1584)
                     /locus_tag="ACZO9R_04970"
     CDS             complement(<1201..1584)
                     /locus_tag="ACZO9R_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HIR0912449.1"
                     /translation="MSLKKRAIEGIVVAGSLVMMSTIAQAADLDGVQVRNAAVQAETD
                     FATVGTAGVVARLDQLEAEAFGDIEAATAGVNQTGRLLVATAEEAVSDTEAAETLDAQ
                     LQQAEATEAQQADAEQSETEAAQSQA"
     gene            complement(1849..2130)
                     /locus_tag="ACZO9R_04975"
     CDS             complement(1849..2130)
                     /locus_tag="ACZO9R_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912450.1"
                     /translation="MLWLEHISPEQLQMGIAVCSLLTVMLLIVTLHKINRIQRYIQKK
                     QTGTDRMESAEFGSAFPAEGVSQCEAAAEGAKKYPADMLIDEVIEEVFS"
     gene            2390..3463
                     /locus_tag="ACZO9R_04980"
     CDS             2390..3463
                     /locus_tag="ACZO9R_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HIR0912451.1"
                     /translation="MKKIVMTGGGTAGHVTPNIALFPALQKEGYEISYIGSYEGIEKR
                     LIEEQGVPYYGISSGKLRRYFDPKNFSDPFKVLKGYHQSVRLLKKLKPDVVFSKGGFV
                     SVPVVLAASRCHIPAIIHESDLTPGLANRIAIPKATKVCCNFPETMAHVPEEKAVLTG
                     TPIRAELLSGDASRAFALCNFTDSAKPTLLVVGGSSGSRAINTAIRDLLPELIKNYNV
                     IHLCGKGNVDASLNAIAGYAQFEYANEELADLFALSSLVISRAGANAICELLALRKPN
                     ILIPLPAAASRGDQILNANSFRSQGFSYVLEEEELTNTTLLEAIDHVFHKKDAYIDAM
                     EHSKVTNSIETIMGLIREASSHK"
BASE COUNT          827 a         1030 c          777 g         1015 t
ORIGIN      
        1 tacaggacac cacaactgcc aataccagaa taaccaaaat caaaaagcta ctaataatcc
       61 tcccattatc taacctccaa ttattttctg tccggatctc taacactatt ttccccgtta
      121 aatatcatat tactatattt gatttgaaaa tacaagaaaa gccgtaggaa atgtcttgtc
      181 atttcccacg gctctgccat gatacctgtt taataaaata cgtggtttcc gattaccaca
      241 cccgcgtgtc ctcgggaagc tctgctaaag caggtagcgc cgcccgtatt gtcagcaccg
      301 ttgattgctt cctgtgccgc ctgaatacaa ctggacttcg gaccgtttgc cgcaatactt
      361 gcaacggatc ctcttcctgc cggcgggaac tgtcctgcct gatatattac accgtaaatg
      421 ctgcccgcat atctgccgga tctcacacgg ttcataacaa ccgcaccgac tgcaagctgt
      481 ccttcgtagc actcgtttcc tgcctcgcac tgaatcagtg cggcaagcag tgtcacgtca
      541 tccaccgaag cggccactgc agaattctga acggtacccg cgctggacac ctgtgcggac
      601 tttgccgctt ctgccgcagc cttcgcagct gctgcctgct cctcctcgat cgtgacagcc
      661 tcaccgagtg caagctccgt cgtcacatac tctgcgctca cataaccggt ggttcccttg
      721 taatcgatcg caacccatcc gtctacggtc tctgcatctg catccagcgg aaaagtcatt
      781 cccgtcgata ccgctgccag cacggacgca tccgggtctg cgctgcttct gatgcggagt
      841 ccgttcgtct ggatctctac ctctgtatca aatgtatcgt tcgcataggt atatgcttcc
      901 gatcccgtca cgcagtagga atttttcaca tatccgtcta cgtttccgga ttccacatgt
      961 gtccaggtat caccagcctc ggtgatctct gccacatctc ccttgtataa tttgccgaca
     1021 atcgctgcat tctcgtctcc ggattccctg atatacagga aatcgtcgac atctgccatc
     1081 aggcggttct gccactcctg ctcttccgca gacatctgat ctgcatccgt gctctgtgca
     1141 tcctccggct gcccgccggc gtctgtctgc tctgcatccg ctgtctgctc nnnnnnnnnn
     1201 tgcctggctc tgcgctgcct ctgtctcgct ctgctcagca tctgcctgct gtgcctctgt
     1261 tgcttcggcc tgctgcagct gtgcatctaa tgtctccgca gcttctgtat ccgaaaccgc
     1321 ttcctccgcc gtagcgacca gaagtcttcc tgtctgattc acaccggctg tcgccgcttc
     1381 aatatcacca aatgcctctg cttctaactg atctaaccgg gcaacaactc ctgctgttcc
     1441 cactgtagcg aagtccgtct ctgcctgtac cgctgcattc cttacctgta ctccgtcaag
     1501 atcagcagcc tgtgcgattg tgctcatcat caccagagat ccggcaacca cgattccctc
     1561 aatcgctctt ttcttcaaac tcatagaacc ctccatcggt gtaatcacat gtttattgaa
     1621 atagtattac acctttgtgc tgttttattc atattttgtt acaattgtta cggatttgtt
     1681 acagcaggaa ttataacaaa ggtactgttt tttgtcaagt tttcggggct ttccgcgtct
     1741 tcatgccgct gtttttctat agaaagaggc agccggaagc cccattttac aagacttccg
     1801 gctgtttttt gtgcattttg ccagtttttc gtttttatga agattttctt aagaaaatac
     1861 ctcttcaata acctcatcaa tcagcatgtc cgccgggtat tttttcgctc cttcggctgc
     1921 cgcctcacac tgcgaaacac cttccgccgg aaaagcgctc ccgaactctg cgctctccat
     1981 tctgtcggtg ccggtctgtt tcttctgtat atatctctgg attctgttta tcttatgtaa
     2041 tgtgacaatc agaagcatca cggtgagcag actgcacact gcaatcccca tctgcaactg
     2101 ttccggtgaa atatgttcca gccagagcat gtccattacc tctctttccg atcagacttt
     2161 ttcctgtccg atttcttcta tattataagg aatctgcacg gatttgaaaa gaggctttcc
     2221 ccgtgatctc gccgcaattt gcgacaatct ttttacatcc catgccggca tcatccgcac
     2281 cctttttcca ttgccgccgc aaagcccgat ctccaagtct tttcttgact tttatgcata
     2341 agggtattat aaataaggga agcagaccat taggaaaggg attcttacta tgaagaaaat
     2401 tgttatgacc ggagggggaa ctgcagggca tgtaacgccg aatattgctt tatttccagc
     2461 tctgcagaag gaaggttatg aaatatccta catcggttcc tatgagggaa tcgaaaaacg
     2521 gctgatcgag gagcagggtg tgccgtatta cggtatctcg tccggaaagc tcaggcgtta
     2581 ttttgatccg aaaaattttt cggatccgtt caaagtttta aaaggctatc atcagtccgt
     2641 ccgtcttttg aaaaagttaa agccggatgt tgttttctca aagggaggct ttgtctccgt
     2701 gccggttgtg cttgcagcaa gccgctgcca tattcctgcg atcatccatg aatcggatct
     2761 cacgccggga cttgccaacc gcattgcaat tccaaaagcc acaaaagtat gctgcaattt
     2821 tccggagaca atggcacatg ttccggagga aaaagccgtg cttaccggca cacccatccg
     2881 agcggaatta ctgtcaggag atgccagccg cgcttttgca ctctgtaatt ttacagattc
     2941 cgcaaagcca acacttctgg tcgtcggcgg aagcagcggc tcccgtgcca tcaataccgc
     3001 catccgggat ctgcttccgg aactgattaa aaattacaat gtcattcacc tgtgcggcaa
     3061 gggaaatgtg gacgcttccc tgaatgccat cgccggttat gcccagtttg agtatgccaa
     3121 tgaggaactt gccgatctgt tcgcgctctc ctcccttgtc atctcccgcg ccggagccaa
     3181 cgcgatctgc gagcttctcg cactgcgcaa accgaatatc ctgattccgc tcccggcagc
     3241 agccagccgc ggcgatcaga tcttaaacgc caactctttc cgttcccagg gattcagtta
     3301 tgttcttgag gaggaggagc tgaccaacac cactctgctg gaagccatcg accatgtctt
     3361 ccacaaaaag gacgcttata tcgatgccat ggagcacagc aaggtcacaa actccatcga
     3421 gacgatcatg ggactgatcc gcgaggcatc ttctcacaaa tagaggttcc tgtataaaaa
     3481 ggaattgtcg caataaacat atcgtatgtt gataagtaat attatcagaa gatcatatcg
     3541 tgggatttca gatggaaatg cagatggaat attcgttgga gaaaaatgtg agcatttctt
     3601 ccggctgaat gtttgcatcg gattccgtgt ctcttccacg tttggacaat ccgacgggt
//
LOCUS       DBMDQJ010000185         3656 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9296_length_3656_cov_1.410689, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000185 DBMDQJ010000000
VERSION     DBMDQJ010000185.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3656
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9296_length_3656_cov_1.410689"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(9..1430)
                     /gene="rhaB"
                     /locus_tag="ACZO9R_04985"
     CDS             complement(9..1430)
                     /gene="rhaB"
                     /locus_tag="ACZO9R_04985"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HIR0912452.1"
                     /translation="MEMTYYLAVDIGASSGRHMLSHLEDGKLVLEEIYRFPNGMTEKN
                     GHKVWDVERLFAEILNGMKQCARLGKIPHSMGIDTWAVDYVLLDGQGRRIGDAAAYRD
                     GRTQGMDEKVYAHITEEELYHRTGIQKQIFNTIYQLAAVREQHPKWLEQADCLLMMPD
                     YFHFLLTGKKVQEYTNATTTQLVSPVTKDWDEQLIRQLGYPRRLFQEIRMPGYELGSL
                     RGEIQKEVGFSCKVILPPTHDTASAVAAVPTQEEETLYISSGTWSLMGTERMTADCSS
                     AARAHNMTNEGGYDCRFRYLKNIMGLWMIQSVRKESAPELGFGEICARAAKETIASRI
                     DVNDARFLAPENMTEEVRRACREAGEQVPQTIGELAAVIYHSLAQCYAGTAEEIEALT
                     GKQYPEIHIVGGGANAQYLNRLTAEYTGKTVEAGPTEATALGNLAVQMIAAGEFEDLS
                     AARACIADSFTMEKFEGEKGETR"
     gene            complement(1553..2542)
                     /locus_tag="ACZO9R_04990"
     CDS             complement(1553..2542)
                     /locus_tag="ACZO9R_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR0912453.1"
                     /translation="MREELLNELMVISEEEKKYRAGQGMVERQLYARESIGEIDRELL
                     LKRGRLITVRPHSRFVDFPEHRHNYVEIMYVVQGSITHIIGGKELTLHKGDVLMLNQQ
                     VRHAIRRAEYEDIGINFIALPEFFEVPLSMLHEKNVLAEFIAGAFGQKNPVSHYLLFR
                     LKEQPQVENLMENMIESMLHEHSDEDVMNQYTMGLVFLYLLNHLENLSHNSSMDYREA
                     IVQAVLGYIKSDCKNANLTKIAKDTHQSVSVLSKLIRQKTGYTFKELLQQRRFETAAH
                     LLTETDLAVEEIALDVGYENLSYFFRQFKSRYGVTPRAYRMMNSRDAGNLYEK"
     gene            complement(2539..>3656)
                     /locus_tag="ACZO9R_04995"
     CDS             complement(2539..>3656)
                     /locus_tag="ACZO9R_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912454.1"
                     /translation="TVLIARYLGEKRPEQIGAVVGGAAIVFALIAVGLFVVMVCFAEP
                     ISVLMQAPAEAVDLTASYVRICGGGIFFIVAYNVLSAVFRGLGDSRSPLLFVLIACIV
                     NVAGDLILVAGFRMDATGAAIATVLAQALSVVFAVLLLLKKDLPFAIVKSDFRWNPQC
                     RNFLRIGLPLALQEFLTQISFLALCAFVNRLGLEASSGYGVACKIVSFAMLVPSALMQ
                     SMASFVSQNIGAGKKKRAKKSMLTGIGVGVTIGCVVFALILLKGDLLAGIFSTDPAVI
                     RNAYDYLKGFSMETIVTAVLFSMVGYFNGNNKTLWVMTQGLIQTLLIRLPMAYVMSSQ
                     PNASLTKIGLAAPVSTTVGIVLNIGFFVYHRRAAERR"
BASE COUNT          806 a         1157 c          794 g          899 t
ORIGIN      
        1 ataacctgtc atctcgtttc tcccttctct ccttcaaact tctccattgt aaacgaatcc
       61 gcaatgcatg ctctcgccgc actgaggtct tcaaattctc ccgccgcaat catctgcacg
      121 gcaaggttgc cgagcgccgt cgcctccgtc ggtcccgcct cgaccgtctt tccggtgtat
      181 tctgcggtaa gccggtttaa atactgcgca ttcgcgccgc ctcccacaat gtgaatctct
      241 gggtactgtt ttcccgtcaa cgcctcgatc tcctctgccg tcccggcata gcactgcgca
      301 agactgtggt agatgaccgc cgccagttcc ccgatggtct gcggaacctg ctcccccgct
      361 tcccgacatg ccctccttac ttcctccgtc atgttctccg gcgccagaaa tcgcgcatcg
      421 ttgacgtcga tacgcgatgc aatcgtctcc tttgcggcgc gcgcacagat ctctccaaac
      481 ccaagttccg gcgcactctc ctttcggaca gactggatca tccagagtcc catgatattt
      541 ttcagatagc ggaaccggca gtcgtaaccg ccctcgtttg tcatgttgtg cgctctcgcc
      601 gcactgctgc aatccgccgt catccgttcg gttcccatga gcgaccatgt gccggagctg
      661 atatagagcg tctcttcctc ctgcgttggc accgccgcca ccgccgaggc ggtatcgtgt
      721 gtgggcggca ggatcacctt gcaggaaaat cccacttctt tctggatctc cccccgcagg
      781 ctccccagtt cataccccgg catccggatc tcctgaaaca accgtctcgg atagcccagc
      841 tgccggatca gctgctcatc ccagtccttt gtcaccggac taaccagctg ggtcgtcgtc
      901 gcattggtgt actcctgcac ctttttcccg gtcagaagaa aatgaaaata atccggcatc
      961 atcagcagac agtccgcctg ctccagccat tttggatgct gctcccttac cgcagccagc
     1021 tgatagatcg tgttaaagat ctgtttttgg attcctgttc tgtggtagag ttcttcctcc
     1081 gtgatatgag catacacttt ttcatccatg ccctgtgtcc tgccgtcgcg gtaggctgcc
     1141 gcatctccga tacgtcttcc ctgcccatcc aaaaggacat agtccaccgc ccaggtgtca
     1201 atccccatgc tgtgcgggat tttccccagt ctggcgcact gcttcatgcc gtttaaaatc
     1261 tccgcaaaca gccgctcgac atcccatacc ttgtgcccgt tcttctccgt cattccgttc
     1321 gggaagcgat agatttcttc caataccagc ttcccgtctt ccagatggga cagcatatgt
     1381 cgtccgctcg acgctccgat atccaccgcc agataatacg tcatctccat ccacctgctc
     1441 ctttctcttt ccttctcatc actgctgtca gcataacaaa aataaggacc gttttttagg
     1501 ctctgattcg actaaaaaac tgtccttatt ttccattctc aaatattctc tattattttt
     1561 cataaaggtt cccggcgtca cgtgaattca tcatgcggta tgcacgcggt gtcacgccat
     1621 accggctctt aaactgccgg aaaaaataac tcagattctc atacccgaca tcaagtgcga
     1681 tctcctccac cgccagatcc gtctccgtga gcagatgtgc cgccgtctca aatctccgct
     1741 gctgcaaaag ctccttgaat gtatatccgg ttttctgccg gatcagcttg cttaacaccg
     1801 agaccgactg atgggtatct tttgcgatct tcgtcagatt cgcatttttg cagtcacttt
     1861 tgatatatcc gagcaccgcc tgcacgatcg cctcccggta gtccatcgag gaattgtggc
     1921 ttaagttctc aaggtgattc aacagatata aaaataccag ccccatcgta tactggttca
     1981 tgacatcctc atcggaatgt tcatgcagca tggattcgat catattttcc atgagatttt
     2041 ccacctgcgg ctgttccttt aagcggaaca gcaggtagtg cgacaccgga ttcttctgtc
     2101 cgaaagcgcc ggcgataaat tccgcaagca catttttctc atgcagcatg gaaagcggca
     2161 cctcaaaaaa ctccggcagc gcgataaaat tgatgccgat atcctcatac tctgccctgc
     2221 ggatggcatg acgcacctgc tggttcaaca tcagcacatc ccccttgtga agtgtcagtt
     2281 ccttcccccc gatgatgtgc gtgatgctcc cctgcacgac atacatgatc tccacatagt
     2341 tgtgccggtg ctccgggaaa tccacaaagc ggctgtgcgg acgcacggtg atgagccgtc
     2401 cccgctttaa cagcagttcc cggtcaatct ccccgatcga ctctctcgca tacagctgcc
     2461 gctcaaccat tccctgtccc gctctgtact ttttttcttc ctctgaaatg accatcagtt
     2521 cgttaagaag ttcttctctc atcgccgctc tgctgccctt ctgtgatata caaaaaatcc
     2581 aatattcagt acgattccca ccgtggtgga caccggcgcc gccagaccga tctttgtgag
     2641 gctcgcattg ggctggctac tcatcacgta agccatcggg agacgtatca ataacgtctg
     2701 gatcagcccc tgcgtcataa cccagagtgt cttgttgttt ccattgaaat agccgaccat
     2761 gctgaaaagg accgctgtca caatcgtctc cattgaaaat cctttcagat aatcatacgc
     2821 gttccggatg accgccggat ctgtcgaaaa aatacccgcc agcagatctc cctttaacag
     2881 gatcagtgca aatactacgc agccgattgt aacacccacg ccgattccgg taagcatgga
     2941 cttctttgcc cgctttttct tccctgctcc tatattctgg gagacgaacg acgccataga
     3001 ctgcatcagc gcactcggta ccagcatggc aaaactaacg atcttgcagg cgacaccata
     3061 accggaggac gcttctagcc ccagacggtt tacaaacgcg cagagcgcca gaaatgaaat
     3121 ctgcgtgagg aactcctgca aagccagcgg cagtccgatc cgaaggaagt tcctgcactg
     3181 cggattccat cggaaatcac tttttacaat ggcaaacgga agatcctttt tcagcagaag
     3241 gagcactgca aataccacgc tgagcgcctg tgccagaacg gtcgcaatcg ctgctcccgt
     3301 ggcatccatg cgaaatcctg ccacgaggat cagatccccc gccacattca caatacacgc
     3361 gatcagtacg aagagtaacg gcgacctgct gtcacccagt cctctgaaaa ctgccgacag
     3421 cacattataa gccacaataa agaaaatacc gccgccgcaa atgcgcacat aggatgccgt
     3481 cagatccacc gcttccgccg gtgcctgcat aaggacagag atcggttccg caaaacatac
     3541 catgacgaca aacagtccga cggcgatcag tgcgaacacg atcgcggcgc ctccgaccac
     3601 cgcgccgatc tgctccgggc gtttttctcc caggtagcgc gcaatcagga cggtca
//
LOCUS       DBMDQJ010000186         3648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9323_length_3648_cov_1.622498, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000186 DBMDQJ010000000
VERSION     DBMDQJ010000186.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9323_length_3648_cov_1.622498"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(42..470)
                     /gene="rplM"
                     /locus_tag="ACZO9R_05000"
     CDS             complement(42..470)
                     /gene="rplM"
                     /locus_tag="ACZO9R_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR0912455.1"
                     /translation="MKTFMASPATIDRKWYVVDAEGMTLGRLASEVAKVLRGKNKPIF
                     TPHIDTGDYVIVVNAEKGKVTGKKLDQKVYYNHSDYVGGMKETTLKEMMAKHPERVIE
                     HAVKGMLPKGPLGREMYTKLFVYAGPDHKHAAQKPEALTF"
     gene            complement(615..1370)
                     /gene="truA"
                     /locus_tag="ACZO9R_05005"
     CDS             complement(615..1370)
                     /gene="truA"
                     /locus_tag="ACZO9R_05005"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR0912456.1"
                     /translation="MIVAYDGTNYKGWQVQPNGITIEEVLNRHLSSLLGEEIAVTGAS
                     RTDSGVHSLGNVAIFDTMTRMPADKISFALNQRLPEDIVVQGSCEVPADWHPRYQNSR
                     KTYEYRILNRTFRMPTRRLDTYFYHHSLDVEKMSRAAVYLEGEHDFKSFCAVNAQVKT
                     TVRTIYACSVTKADDIITIRVTGNGFLYNMVRIIAGTLIQVGGGQIEPEQIEQILAAR
                     DREAAGPTAPAHGLTMMGIEYMEEKDIDTQGVV"
     gene            complement(1417..2229)
                     /locus_tag="ACZO9R_05010"
     CDS             complement(1417..2229)
                     /locus_tag="ACZO9R_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T family protein"
                     /protein_id="HIR0912457.1"
                     /translation="MLRDITLGQYYPADSVIHKLDPRVKLFSTMIYIISLFCFRGVAA
                     LAIATVFLITVIKLSKVPFKFMVKGLKAIMILMLITAVFNLFLTPGEALVSVWRFTIT
                     KEGVTSAVLMAIRLTYLILGTSIMTLTTTPNQLTDGLEKALMPLSKIGVPVHAIAMMM
                     SIALRFIPILIEETDKIMKAQMARGADFESGNLLKKVKSMIPLLVPLFVSAFRRADDL
                     AMAMEARCYNGGEGRTKMKPLRYEGRDRLSYLIMWLYLALIILCRIFVPWPQ"
     gene            complement(2316..3194)
                     /locus_tag="ACZO9R_05015"
     CDS             complement(2316..3194)
                     /locus_tag="ACZO9R_05015"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HIR0912458.1"
                     /translation="MSIILDKVNFVYSEETAYQIQALKDVNLEIKDGQFIGIIGHTGS
                     GKSTLIQHLNGLMKATSGTIYFHGQDIYEEDFDLRELRNRVGLVFQYPEHQLFETTIF
                     DDVCFGPKNQGLSKEEAGLRAFEALRSVGMPEELYYQSPFDLSGGQKRRVAIAGVLAM
                     KPEVLILDEPTAGLDPAGRDEILDLVARMHRERNMTVILVSHSMEDVAKYVERIIVMN
                     HGQVMFDDTPKEVFRHYKELETIGLAAPQVTYLMHELKEHGLSVDTEATTVEEARASL
                     MQALTGIDSGRSDIHR"
     gene            complement(3197..>3648)
                     /locus_tag="ACZO9R_05020"
     CDS             complement(3197..>3648)
                     /locus_tag="ACZO9R_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0912459.1"
                     /translation="SYRHHSPNKLSGGQKQRVAIAGVMAMEPKCIVLDEPTAMLDPNG
                     RKEVIRTAHELNRKKGVTIILITHYMEEVVDADKVIVMDQGKVVMQGTPREVFSQVGK
                     LKEYRLDVPQVTILADLLRQSGIDVPVGVLHRQELVDAILRVAQIGE"
BASE COUNT          808 a         1092 c          793 g          955 t
ORIGIN      
        1 tccgtaatat tttgcgttag ccaatgtctt ttacctcctc attaaaatgt taaagcttct
       61 ggcttctgag ctgcgtgctt gtgatccgga cctgcgtata cgaataactt tgtatacatc
      121 tctcttccaa gaggtccctt cggaagcatt cccttaacag catgctcgat cactctctcc
      181 gggtgcttag ccatcatctc ttttaatgtg gtctctttca taccgccaac ataatcagag
      241 tgattgtagt aaaccttctg atctaacttc ttaccggtaa cctttccctt ctcagcgttc
      301 actacgatta cgtaatcacc tgtatcgata tgtggtgtaa agattggttt attctttcct
      361 cttaatacct tggcaacttc agatgccagg cgtcctaatg tcataccttc agcgtcaact
      421 acataccatt ttctatcaat ggtagctggg ctagccataa aagtcttcat cgttattcct
      481 cctaaaaaat gctgttcatc tcaggcagcg tgaatgtccg gcacactgtc ctccatgtca
      541 tattgtttat agttaaatgg cactccgggg ctttggaatg acatttattc actatcacag
      601 acttaaatat tatattacac gactccttgc gtgtcaatat ctttttcttc catatactca
      661 attcccatca tagtcagtcc gtgcgccggt gcggtcggtc ccgcagcctc cctgtcgcgc
      721 gccgcgagga tctgctctat ctgctccggc tcgatctgac ctccgccgac ctgtatcagc
      781 gtcccggcaa taatgcggac catattgtac aaaaaaccgt tgccggtcac ccggattgtg
      841 atgatgtcgt ccgcttttgt gacgctgcac gcatagattg tccggaccgt ggtcttcacc
      901 tgcgcattga ccgcacagaa gctcttaaaa tcgtgctccc cttccaggta caccgccgcc
      961 cggctcatct tctccacatc caggctgtgg tggtaaaaat aagtatccag ccgtctcgtc
     1021 ggcatacgga atgtccggtt caaaatgcgg tactcatatg tcttgcggct gttctgatag
     1081 cgcggatgcc agtccgcagg aacctcgcag gacccctgca cgacaatatc ctcaggcaga
     1141 cgctgattca gtgcaaacga aatcttatcc gccggcatgc gcgtcatcgt atcgaaaatt
     1201 gccacgtttc ccagcgaatg gacgccggaa tctgtgcggc ttgcgccggt cactgcgatc
     1261 tcctccccca aaagtgagga aagatggcgg ttcagcacct cctcgatcgt gattccgttg
     1321 ggctgcacct gccatccttt ataattcgtg ccgtcgtacg ccacgatcat ttttacacgt
     1381 tttacctctt tctccaaacc atttctcctt gcagctttat tgtggccacg gcacaaagat
     1441 tctgcacaga ataatgagcg ccagatacag ccacatgatc agataggaca ggcggtcgcg
     1501 tccctcatag cgcagcggct tcatcttcgt tctgccctct ccgccgttat agcagcgcgc
     1561 ctccatcgcc attgcaagat catccgcacg ccggaatgct gacacgaaca gcggcaccag
     1621 aagcgggatc atgcttttga ctttttttaa cagattgcca ctctcaaaat cggctccgcg
     1681 tgccatctgc gctttcatga tcttatccgt ctcctcaatc agaatcggaa taaagcggag
     1741 cgcaatcgac atcatcattg caatcgcgtg caccggaaca ccgatcttag aaagcggcat
     1801 cagcgctttt tccagaccat ctgtcagctg gttcggcgtt gtggtcagcg tcatgattga
     1861 ggttccgaga atcagatagg tcaggcggat cgccatcagc accgcgctgg tcacgccttc
     1921 cttcgtgatc gtaaaacgcc agacgcttac cagtgcctca ccgggcgtca aaaacagatt
     1981 gaacaccgcc gtgattaaca tcagaatcat gattgccttt aatcccttta ccatgaactt
     2041 aaacggcact ttcgacagct ttatgactgt aatcagaaat accgttgcaa tcgcaagcgc
     2101 tgccacgcca cggaagcaga acagggatat gatatagatc attgtactaa acagtttcac
     2161 acgcgggtcc agtttatgga tcacagaatc tgccggataa tactggccca gtgtaatatc
     2221 tctcaacatg tataaacttc ctccctaccg gaattcttca tcggcgccca ccggcgactc
     2281 tttcttccgt aaaatgattc ctgccgcggc acaccttacc ggtgaatatc gctcctgccg
     2341 gaatcaattc ccgtcaatgc ctgcatcagg ctggctctgg cttcctccac cgtcgtcgcc
     2401 tccgtatcca cggaaagccc gtgctccttc agttcgtgca tcaggtaagt cacctgtgga
     2461 gccgcgagtc cgatcgtctc cagctcctta tagtgccgga acacttcctt cggtgtgtcg
     2521 tcgaacatca cctgtccatg gttcatcacg ataatgcgct ccacatactt ggcgacatcc
     2581 tccatactgt gcgacaccag gatgaccgtc atgtttcgct cgcggtgcat ccgtgccaca
     2641 aggtctaaga tctcgtctct tcctgccggg tcaagtcccg cggtcggctc atccaggatc
     2701 agcacctccg gcttcatggc aagcactccg gcgatcgcca cacggcgctt ctgccctccc
     2761 gagagatcaa acggcgactg gtaataaagc tcctccggca taccgacact gcgcagtgcc
     2821 tcaaacgccc gcagtcccgc ctcttccttg gaaagtccct ggttcttcgg accaaagcag
     2881 acgtcatcaa aaattgtcgt ctcgaaaagc tgatgctccg gatactggaa caccagtccc
     2941 acccggttgc gcagttctct caggtcaaaa tcttcctcat aaatatcctg tccatgaaaa
     3001 tagatcgttc ccgaggttgc cttcatcaga ccgttcaggt gctggatcag cgttgacttg
     3061 ccggaaccgg tatgtccgat gatgccgatg aactgtccgt ccttaatctc aaggttcaca
     3121 tcctttaacg cctgaatctg gtacgcggtc tcctcactat atacaaaatt cactttatct
     3181 aaaataattg acatttctac tctccgatct gtgccacacg aagaatcgcg tccacaagtt
     3241 cctggcggtg cagcacaccg acgggtacat caatgccgga ctggcgcagc agatctgcta
     3301 aaatcgtcac ctgcggcaca tccagtctgt actccttgag ttttcccacc tgggagaaca
     3361 cttcccgcgg tgttccctgc atgacgactt ttccctgatc catcacgatc accttgtcgg
     3421 cgtccacgac ttcctccata taatgtgtga tcaaaatgat cgtcacgcct tttttccggt
     3481 tcagttcatg cgccgtgcgg atgacttcct tccgcccgtt cggatcaagc atggcagtcg
     3541 gctcgtcgag cacaatgcac ttcggttcca tcgccatgac cccggcgatc gccacgcgct
     3601 gcttctgtcc gccggagagc ttgttcggcg aatggtggcg gtacgaga
//
LOCUS       DBMDQJ010000187         3648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9325_length_3648_cov_1.561883, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000187 DBMDQJ010000000
VERSION     DBMDQJ010000187.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3648
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9325_length_3648_cov_1.561883"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="ACZO9R_05025"
     CDS             complement(<1..316)
                     /locus_tag="ACZO9R_05025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009247287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0042777 - proton motive force-driven plasma
                     membrane ATP synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR0912460.1"
                     /translation="MANTREIQSRMKSIQDTMKITNAMYMISSTKLRKAKKNLEETEP
                     YFYTLQTMVSNLEKAEAEKRMVEIVNYVKVQCSTYTHYNESSESKSLLRAIESARQMS
                     TNI"
     gene            complement(353..1867)
                     /gene="atpA"
                     /locus_tag="ACZO9R_05030"
     CDS             complement(353..1867)
                     /gene="atpA"
                     /locus_tag="ACZO9R_05030"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR0912461.1"
                     /translation="MGSINSEEIISILKTEIENYDMVTGEQEVGSVIWVGDGIATIYG
                     IEHAMYGEIVIFENGVRGMVQDIKRDQIGCIILGKDTGIKEGTKVTRTKKKAGVPVGN
                     AYLGRIVNALGAPIDGKGEIPADDYRPIEQEAPGIVDRQSVKQPLETGILAIDAMFPI
                     GRGQRELIIGDRQTGKTSIAVDTILNQKGKDVICIYVAIGQKASTVAKLVNTLTKNDA
                     MDYSIILSSTASESASLQYIAPYAGTAMAEYFMHQGKDVLIVYDDLSKHAVAYRALSL
                     LLERSPGREAYPGDVFYLHSRLLERSSKLSDELGGGSITALPIIETQAGDVSAYIPTN
                     VISITDGQIFLESDLFFSGMRPAVNVGLSVSRVGGAAQTKAMKKASGTIRIDLAQYRE
                     MEVFTQFASDLDDMTKEQLQHGKCLMELLKQPLCRPLSMAEQVMTLCAANGRVMLDVP
                     VDHVKDFQMQFLSYMKEQHSDIVRQLETTKALDDELNKQICDAAEEFKKEFLQK"
     gene            complement(1872..2375)
                     /gene="atpH"
                     /locus_tag="ACZO9R_05035"
     CDS             complement(1872..2375)
                     /gene="atpH"
                     /locus_tag="ACZO9R_05035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR0912462.1"
                     /translation="MTQTANNYGTVLFELGVEKETVEEMKRIFSLTGELPRVLDCPVV
                     SGREKHRLIEQLFPKEVWNFLKEMCDHGNVSEMEDVFKAYTRCYDEANGILKTVMYCA
                     GTPDEEQLAQIKAYLAAKHHRETVELTICTQPELIGGFVLETGDLVEDYSVRGRIKNL
                     EQKLTWR"
     gene            complement(2356..2862)
                     /gene="atpF"
                     /locus_tag="ACZO9R_05040"
     CDS             complement(2356..2862)
                     /gene="atpF"
                     /locus_tag="ACZO9R_05040"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR0912463.1"
                     /translation="MQLLRIDWNVIFTIVNLIVLYLGLRKFLIGPVTKVMDQRKQLIE
                     GQMADARHVNEQANELKAQYEDALKQAHEESAQIVEKARKSAQAEYESRMDAADAEAQ
                     KIIANAHKSIDLEREKTVQELQSQIAGLAMAATAKVLGDTDQAAQNKLMYEQFLAKTG
                     GLNDTNSK"
     gene            complement(2882..3106)
                     /gene="atpE"
                     /locus_tag="ACZO9R_05045"
     CDS             complement(2882..3106)
                     /gene="atpE"
                     /locus_tag="ACZO9R_05045"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR0912464.1"
                     /translation="MTSLIAIGAGIAALTGIGAGIGIGLATSKATDAIARQPEADGKI
                     TKALLLGCALAEGTAIFGMVVAILIILFLG"
     gene            complement(3138..>3648)
                     /locus_tag="ACZO9R_05050"
     CDS             complement(3138..>3648)
                     /locus_tag="ACZO9R_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015525578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR0912465.1"
                     /translation="KKQVVLETVVGGLYNFFYDLLGEDGKGYIPYLMTVGLYIGVANM
                     IGLFGLKSPTKDVGVTGALALMSIVLIEFSGVHKKGVKGFLKSFAEPIPLMLPMNILE
                     IFIRPLSLCMRLFGNILGAFVIMELIKIVAPLLVPVPLSLYFDLFDGFIQAYVFVFLT
                     SLFIKETIE"
BASE COUNT          848 a         1019 c          756 g         1025 t
ORIGIN      
        1 taatattggt actcatctgt ctggcacttt caatggcacg gaggagactt ttggattccg
       61 aagattcatt ataatgggtg taggtggagc actgcacttt gacataattt acaatctcta
      121 ccattctttt ctctgcttcc gctttctcca aattgctgac catagtctgc agagtataga
      181 agtaaggctc cgtctcttcc agattcttct tggctttccg aagctttgtg gaagagatca
      241 tatacatggc attggtgatc ttcatagtat cctgaatgct tttcatacga ctttgtattt
      301 ctcgcgtatt tgccatgtta acctcctata ttactatcac tgtttgcaaa tattattttt
      361 gcaaaaattc ctttttaaat tcctcagctg catcgcagat ctgcttattc agctcgtcat
      421 ccagtgcctt ggtggtctct aactgacgaa cgatgtcact gtgctgctcc ttcatgtaac
      481 ttaagaactg catctggaaa tccttcacat gatctacagg cacgtccagc attactctgc
      541 cgttggctgc acacagggtc ataacctgtt ctgccatgct taaaggtctg cacagaggct
      601 gctttaacag ctccatcagg cattttccgt gctgtaactg ctcttttgtc atgtcgtcca
      661 gatcggatgc aaactgtgtg aacacttcca tctcacgata ctgcgccaga tcaatacgga
      721 tcgtaccgga cgctttcttc atcgccttgg tctgcgccgc accgccgaca cgggatacgg
      781 agagaccgac gttgactgcc ggacgcatac ctgagaaaaa cagatcactc tccaaaaaga
      841 tctgaccgtc tgtgatggag atcacgttgg tcggaatgta tgcggataca tcgcccgcct
      901 gcgtctcaat gatcggcagc gccgtgatgg aacctccgcc cagctcatcg gaaagcttgc
      961 tggaacgctc cagcagtctg gaatgcagat aaaatacgtc tcccggataa gcctcacgtc
     1021 ccggagaacg ctccagcaaa agggacagtg cacgatatgc cactgcgtgc ttggagaggt
     1081 catcatatac gatgaggaca tccttgccct gatgcatgaa atactctgcc atagcggttc
     1141 ccgcatacgg tgcaatgtac tgtaacgatg ccgactcgct ggctgtcgat gataaaatga
     1201 tggagtagtc catcgcatca ttcttcgtca atgtattcac aagctttgct acggtcgatg
     1261 ccttctgacc gatcgccaca taaatacaga tcacatcttt tcccttctgg ttcaagatgg
     1321 tatctactgc gatcgacgtc ttaccggtct gtctgtcgcc gataatcagc tcccgctgcc
     1381 cgcgtccgat cgggaacatg gcgtcaatcg ccagaatacc ggtctcgagc ggctgcttga
     1441 ccgactgacg gtcaacaatg cccggcgctt cctgctcgat cgggcggtaa tcgtccgccg
     1501 gaatctctcc cttgccgtcg atcggcgctc ccagtgcatt tacaattctt ccaagatagg
     1561 catttccgac tggaacaccc gccttcttct tcgtgcgtgt gacctttgtg ccctctttga
     1621 tgccagtgtc ttttccgaga atgatacagc cgatctgatc gcgcttgata tcctgcacca
     1681 tacctctgac gccattctcg aagatcacga tctcaccata catggcatgc tcaatgccat
     1741 agatggtcgc aattccgtct cctacccaga tgacagagcc gacttcctgc tcaccggtca
     1801 ccatatcata attttctatc tctgttttca gtatggagat aatctcctct gaatttatgg
     1861 aacccacgtc atcacctcca tgtcaatttt tgttctaaat tcttgatccg cccccggacg
     1921 ctgtaatctt ccaccagatc tcccgtctcc agcacaaatc cgccgatcag ttccggctgt
     1981 gtgcagatgg taagctccac cgtctctctg tggtgctttg cggcgagata tgctttgatc
     2041 tgtgcaagct gctcctcatc cggtgtaccc gcgcagtaca taacggtctt taaaattcca
     2101 tttgcctcat cgtagcagcg cgtatacgct ttaaagacat cctccatctc cgatacgttt
     2161 ccgtggtcgc acatttcttt taagaaattc cagacctctt tcggaaacag ctgctcaatc
     2221 agcctgtgtt tttccctgcc ggataccacc ggacagtcca gcacgcgcgg caattcgcca
     2281 gtcagtgaga aaatccgctt catctcttcc acagtctctt tctccacgcc cagctcaaaa
     2341 agaaccgttc cgtaattatt tgctgtttgt gtcatttaaa ccacccgttt tcgccaaaaa
     2401 ctgttcatac ataagcttgt tctgcgctgc ctgatccgtg tcgcccaata ccttagcggt
     2461 tgccgccatg gcaagtcccg caatctgcga ctgcagctcc tgcacggttt tctcacgctc
     2521 aaggtcaatc gacttgtgtg cgttggcgat aattttctgc gcctctgcat ccgctgcatc
     2581 catacggctc tcatactccg cctgtgcgct ctttctcgct ttttcaacga tctgcgccga
     2641 ttcctcatgc gcctgcttta aggcgtcctc atactgcgct ttcagctcat ttgcctgctc
     2701 atttacatgg cgtgcgtccg ccatctgccc ttcgatcagc tgcttgcgct gatccatcac
     2761 tttcgtgacc ggtccgatca aaaatttgcg gagccccaga tataatacga tcagattgac
     2821 gatggtaaag atgacattcc agtcgattct aagtaactgc atactctacc ataacctttc
     2881 cttagcctaa gaataagatg attaagattg ctactaccat accgaaaatt gcagtaccct
     2941 ctgccagtgc acatccaagt aacagtgcct ttgtgatctt tccgtcagcc tccggctgtc
     3001 ttgcgatagc gtctgttgcc ttggaagttg caagtccgat accgatacct gctccaatac
     3061 ctgtaagtgc tgcgattcct gctccgattg cgattaaaga tgtcataata atgttctcct
     3121 tttctttttc gatttgatta ctcgatggtt tccttaataa aaagtgatgt taaaaataca
     3181 aatacatatg cctgaatgaa tccgtcaaac aggtcaaagt agaggctgag cggtaccggc
     3241 acaagtaacg gtgccacgat cttgatcagc tccatgataa cgaatgcgcc taagatatta
     3301 ccgaaaagtc gcatacacag tgataacggc cggataaata tttccagaat attcatcggc
     3361 agcataagcg ggatcggttc cgcaaagctc tttaaaaagc ccttgacgcc ttttttatgt
     3421 acgcccgaaa attcaatcag gacgatactc atcagtgcaa gagcaccagt gacgcccaca
     3481 tccttggttg gagacttgag accgaacaga ccgatcatat tggccacgcc gatatacagc
     3541 cctacggtca tcagatatgg gatatacccc tttccatcct cccccaggag atcataaaag
     3601 aagttgtata agcctcccac taccgtttcc agtacaacct gtttcttg
//
LOCUS       DBMDQJ010000188         3645 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9330_length_3645_cov_1.367099, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000188 DBMDQJ010000000
VERSION     DBMDQJ010000188.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9330_length_3645_cov_1.367099"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(168..848)
                     /locus_tag="ACZO9R_05055"
     CDS             complement(168..848)
                     /locus_tag="ACZO9R_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HIR0912466.1"
                     /translation="MKVILVNGSPREKGCTYTALCEVAGALEKNGIETEIFQVGAKPI
                     AGCIGCNVCLKNGRCFVDDPVNEFVEKAKTADGFVFGSPVHYAAASGAITSFLDRAFY
                     GKGNVFAGKPGAAVVSCRRGGAASAFDQLNKYFTINSMPVVSSQYWNQVHGNTPDEVR
                     QDAEGLQTMRTLGSNMAWLLKCIQAGAAAGITFPEREPANKFYSVKVQWKLREIKINL
                     KHRNSHLM"
     gene            complement(984..2225)
                     /gene="fliB"
                     /locus_tag="ACZO9R_05060"
     CDS             complement(984..2225)
                     /gene="fliB"
                     /locus_tag="ACZO9R_05060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF038110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin lysine-N-methylase"
                     /protein_id="HIR0912467.1"
                     /translation="MILKVPSYYEKFHCIADQCKDNCCYGWEIDIDEGTMDYYRSLGG
                     ELGKEITSHIREGEENTMIMREDGYCPFLNEKKLCDICIKMGEEALSEICTEFPRFTM
                     EYADVREKILSLACEEVGNIMFSTDEKITWQEHEMPDFCGTGDDAEAAYDADGALYGN
                     DEAACEDDTSDGEGYDDDEEVSVDADQLNAVSKAAVALLQNREKPVAERAAEYLLYCK
                     RMQQELYGYHTGGEVSGQEQEQRESRLIAECREKEVTPGEAYAAFLARMERYEQLEVF
                     DQNWEEEKKRLRAAFHEDNYLECIRNFHAAQQGREYEYEHLLVYFTFRYFMRSYYDDN
                     ILNKAQFAVAGILMIRDMDALRFLENGGTFSVEDRVAVAKLYSKEVEHSEENMEFLEE
                     DFQFEDIFAPEQMIRQLFFAS"
     gene            complement(2316..2567)
                     /locus_tag="ACZO9R_05065"
     CDS             complement(2316..2567)
                     /locus_tag="ACZO9R_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR0912468.1"
                     /translation="MKITWKTLLALFFYTLGVVGWFYVGGWMILRGPVRGVILAHMAG
                     DLSLIKLAVALVQGFVYLSLAGGVWCIGYMLSDHFRKDE"
     gene            complement(2649..3167)
                     /gene="nrdG"
                     /locus_tag="ACZO9R_05070"
     CDS             complement(2649..3167)
                     /gene="nrdG"
                     /locus_tag="ACZO9R_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HIR0912469.1"
                     /translation="MNYSAIKYCDIANGTGVRTVLFVSGCRNHCKDCFQPETWAFEYG
                     NPFTGEVEDEIIASLKPDYIRGLTLLGGDPFEPENQKTLLPFMRRVKAECPGKDVWAY
                     TGYVLDRDLVPGGKCCTADTAELLHMIDVLVDGPFVTELHDISLLFKGSSNQRVIDCA
                     EYCRSGEIRELM"
     gene            complement(3239..>3645)
                     /gene="nrdD"
                     /locus_tag="ACZO9R_05075"
     CDS             complement(3239..>3645)
                     /gene="nrdD"
                     /locus_tag="ACZO9R_05075"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR0912470.1"
                     /translation="NSYHVHVTEQINAFDKLKFESQFQELSPGGAISYVEVPNMQNNL
                     EAVLSVMQYIYENIMYAELNTKSDYCMECGYAGEIKIVTDRDGKLVWECPNCGNRDQN
                     KMSVARRTCGYIGTQFWNQGRTQEIKERVLHL"
BASE COUNT          822 a         1124 c          720 g          979 t
ORIGIN      
        1 ggcgtatcac gatgagatat accatagatc caactatgac ttacagcgaa gcagttctct
       61 tttatgaaga atatctcact gctgccaagg ctgccgggaa aaagccggta tctttcctgc
      121 actttatcac aggtcgttac tagaaggctt ttcccttctt ttttgagcta catcagatga
      181 gaatttctgt gttttaaatt tatcttaatt tctcttaatt tccactgaac ttttaccgaa
      241 taaaatttgt ttgcaggttc ccgctccggg aatgtgattc ccgctgccgc tcctgcctga
      301 atacatttga gcagccatgc catattgctg cccagcgtgc gcatggtctg cagtccctct
      361 gcgtcctgcc gtacctcgtc cggcgtattt ccgtgtacct ggttccagta ctgggaggaa
      421 accaccggca tggaattgat cgtgaaatat ttgttcagct gatcaaacgc ggatgccgcg
      481 ccgccgcgtc tgcagctcac cactgctgcc cccggctttc cggcaaacac atttcccttt
      541 ccgtagaatg cgcggtcgag aaacgaggtg atggcaccgg atgccgccgc ataatgcacc
      601 ggcgagccga atacaaaacc atccgccgtc tttgctttct caacaaattc attgaccgga
      661 tcatccacaa agcagcgtcc gttcttaaga cagacattgc agccgatgca gccggcgatc
      721 ggctttgcac ccacctgaaa aatctcggtc tcaatgccgt tcttctcaag agcccctgcc
      781 acctcgcaga gcgctgtata agtacatccc ttttccctcg gacttccgtt taccaatatt
      841 actttcatgt tctttcctcc atcctggcag atttcctgcc ttctattcta tgccactttc
      901 cctgtgatcg tacgcttatg aaatataaag tgcttcctgc cttgggtggg tcggaatcgc
      961 tccattgatg ttctccgtct tccctatgaa gcaaagaaaa gctgccgtat catctgttcc
     1021 ggtgcaaaaa tatcctcaaa ctggaaatcc tcctccagga attccatatt ttcctcggaa
     1081 tgttctactt cctttgaata cagctttgcc actgccaccc ggtcttccac ggagaatgtt
     1141 cctccgttct ccagaaaacg cagtgcatcc atatcgcgga tcatcagaat acccgccacc
     1201 gcaaactgtg ccttatttaa aatgttgtca tcataatacg atctcataaa ataccggaat
     1261 gtaaagtaga ccagcagatg ctcatactca tattcccttc cctgctgcgc tgcgtgaaag
     1321 ttcctgatac attccagata attgtcctca tggaatgctg cacgaagacg tttcttttct
     1381 tcctcccagt tctggtcgaa cacttccagc tgttcgtagc gctccatgcg tgcaagaaac
     1441 gccgcatacg cctccccagg cgttacctct ttctccctgc actcggcaat cagccggctc
     1501 tcacgctgct cttgctcctg cccgcttact tccccgcctg tgtggtatcc gtaaagttcc
     1561 tgctgcatcc tcttacaata tagaagatac tccgccgcgc gctctgccac cggcttctcc
     1621 cggttctgaa gcaatgccac cgccgcctta gaaacggcat tcagctgatc ggcatccaca
     1681 gacacttcct catcgtcgtc atacccctca ccgtcggagg tatcatcctc acacgctgcc
     1741 tcatcattac cgtacaatgc cccgtcggca tcataagctg cctcggcgtc gtccccggtc
     1801 ccgcagaaat ccggcatctc atgctcctgc caggtgatct tctcgtcggt ggaaaacata
     1861 atgtttccga cctcctcaca tgccagactt aagatcttct cccggacgtc cgcatactcc
     1921 atggtaaagc gtggaaactc ggtgcagatc tcgctcagtg cttcctcgcc catcttaatg
     1981 cagatgtcgc agagcttttt ttcatttaaa aacgggcagt acccgtcctc gcgcataatc
     2041 atggtgttct cctcaccctc cctgatatgc gaggtaatct cttttcccag ctctccgccc
     2101 agactgcggt agtaatccat cgtaccctcg tcgatgtcga tctcccagcc atagcagcaa
     2161 ttatccttgc actgatctgc aatacagtga aacttttcat agtaagacgg tacttttaat
     2221 atcatcgttt gctcctgtct gcgcctcttc ctgcgccatt ttatatccat ttacggtgca
     2281 aaaaggtgcg tcatccgccg cacctttttg tagtcctact catctttccg gaaatgatcg
     2341 ctcaacatat agccgataca ccagacgcct cccgccagtg agagatacac aaacccctgc
     2401 accagtgcga ccgccagctt gatcagagac aaatctcctg ccatatgcgc cagaatcact
     2461 cctctgaccg gtcctcttaa gatcatccat cctcctacat agaaccagcc aacaactccc
     2521 agcgtgtaaa aaaacaacgc cagcaatgtc ttccatgtta ttttcatatc taattctccc
     2581 ttgttggtcc tgcaataccg cgtctattgt aacacaaacc gcaggaagat gcaaaatcct
     2641 tccttcttct acatcagctc ccggatctct cccgaccggc aatactctgc acagtcgatc
     2701 acccgctggt tggaagaccc cttaaacagc agggatatat catgaagctc cgtcacaaac
     2761 ggtccgtcca ccagcacatc gatcatgtga agcagctccg cggtatctgc ggtgcagcat
     2821 tttccgcccg gcacaagatc ccggtctaac acatagccgg tgtatgccca cacatccttc
     2881 cccgggcact ccgccttcac tctccgcata aacggaagca gcgtcttctg attctccggt
     2941 tcaaaaggat ctcctccgag cagcgtcagt ccccggatgt aatccggctt caaggacgca
     3001 atgatctcat cttccacctc gccggtgaaa ggattcccgt attcaaaagc ccacgtctcc
     3061 ggctgaaaac agtctttgca gtgatttctg cagcccgaca caaagagaac ggtgcgcaca
     3121 ccggttccgt ttgcaatatc acaatatttg attgccgaat aattcatatc ttttctcctc
     3181 aacagggaaa gaaagactgt ggctgcacaa tcccgtgcac accacagtcc ccctgttttt
     3241 ataaatgtaa tacccgttcc ttgatctcct gtgttctgcc ctgattccag aactgcgttc
     3301 caatatagcc gcaggtacgt ctcgccacgc tcatcttatt ctgatcgcgg ttgccgcagt
     3361 tcgggcactc ccataccagc ttgccgtccc ggtctgtcac aatcttgatc tctccggcat
     3421 atccgcactc catgcagtag tcactctttg tgttgagttc cgcgtacatg atattttcat
     3481 agatgtactg catcacggaa agcaccgctt ccagattgtt ctgcatattc ggaacctcca
     3541 cgtagctgat tgctccgccc ggggacagtt cctggaactg tgactcgaac tttaacttat
     3601 cgaacgcgtt gatctgctcg gtcacatgta catgatagct gtttg
//
LOCUS       DBMDQJ010000189         3632 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9381_length_3632_cov_1.541206, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000189 DBMDQJ010000000
VERSION     DBMDQJ010000189.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9381_length_3632_cov_1.541206"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /gene="mnmA"
                     /locus_tag="ACZO9R_05080"
     CDS             complement(<1..891)
                     /gene="mnmA"
                     /locus_tag="ACZO9R_05080"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR0912471.1"
                     /translation="MAGKKVVVGMSGGVDSSVAAYLLKQQGYDVIGVTMQIWQDEDQF
                     TQAESGGCCGLSAVDDARRVADRLEIPYYVMNFKREFKKNVMDYFTAEYLKGRTPNPC
                     IACNRYVKWESLLQRSLEIGADYIATGHYARVEQLSNGRYAIRNSVTAAKDQTYALFN
                     LTQKQLSHTLMPVGDYTKDEIRAIAEDIGLMVAHKKDSQEICFIPDNDYAGFIDRECG
                     EMVPPPGNFVSVDGEILGRHKGITHYTIGQRKGLGIAFGYPVFVTEISPETNEVVLGS
                     DKDVYTNRLYANQLNFMALSD"
     gene            complement(969..1748)
                     /locus_tag="ACZO9R_05085"
     CDS             complement(969..1748)
                     /locus_tag="ACZO9R_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912472.1"
                     /translation="MASISGYDSSSLGVLFSSLNRNNTTSSLLGGGSDLLGINYSDYA
                     TIRNGSYFKLLSAYYSKSDSTDSATKVSPSISTAKDDAKTLTRIESAAESTEKSLSKL
                     TATGKDSVFNKVEKTAEDGTKTQEYDRDAIYKAVKSYVDDYNSLLDRADDSKTKSILR
                     AANSLKSNARANEKMLEKAGITVDADGRLSVDEKSFEKADMDKLKSLFTTHGSYATQT
                     NVDLLKIASNAKSEAAKANTYSKNGMYTYNYSTGDLYNSGI"
     gene            complement(2144..2782)
                     /locus_tag="ACZO9R_05090"
     CDS             complement(2144..2782)
                     /locus_tag="ACZO9R_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR0912473.1"
                     /translation="MRVKAIMMPFSKLACISVENTVEEAMKIIDEQGLLSLPVVDGQQ
                     FVGVLSKQFLFEEFFRNYTGTREEFAQRKVMEFMKTRIETIGENTRIEEAAAMFITSK
                     VRFIPITNEQNKLLGIVTQQAVFKEYQKIFGNKHNSMAIYTYDIRGVLGKICELIAKE
                     GGDICNMMVIPTDVMDLAEIFLRVEAPDFERVIRALERHGYDVRDLKYNDQK"
     gene            complement(2826..3503)
                     /locus_tag="ACZO9R_05095"
     CDS             complement(2826..3503)
                     /locus_tag="ACZO9R_05095"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR0912474.1"
                     /translation="MSMIDNDWLPAIQGEFRKPYYRELYTFVREEYSRTVVYPPAEDI
                     FNAFHFTPLSEVKVLLLGQDPYHNVNQAHGLSFSVLPEQKEIPPSLQNIYKELHDDLG
                     CYIPNNGYLKKWADQGVLLLNTVLTVRAHQANSHQGRGWEQFTDAVIQAVNAQDRPIV
                     YLLWGRPAQSKIPMLTNPKHLILTAPHPSPLSAYRGFFGCRHFSKANAFLEEHGIAPI
                     DWQIENI"
     gene            complement(3550..>3632)
                     /locus_tag="ACZO9R_05100"
     CDS             complement(3550..>3632)
                     /locus_tag="ACZO9R_05100"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516158.1"
                     /note="catalyzes the conversion of
                     5-[(5-phospho-1-deoxyribulos-1-ylamino)methylideneamino]-
                     1-(5-phosphoribosyl)imidazole-4-carboxamideand glutamine
                     to imidazole-glycerol phosphate,
                     5-aminoimidazol-4-carboxamideribonucleotide and glutamate;
                     the HisF subunit acts as a cyclase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR0912475.1"
                     /translation="SLFHYKELEIMDLKNDLAARGVSVRR"
BASE COUNT          844 a         1025 c          743 g         1020 t
ORIGIN      
        1 gtcggagagc gccataaagt tcagctgatt cgcatacagc cggtttgtat acacatcttt
       61 atcacttccg agaactacct catttgtctc cgggcttatc tctgtcacaa acaccggata
      121 tccaaacgca atcccgagtc ctttgcgctg tccgatcgta taatgtgtaa ttcccttgtg
      181 ccgccctaaa atctctccgt ccaccgacac aaagttgccc ggcggcggaa ccatttcccc
      241 gcactctctg tctataaatc cggcataatc attgtccggt ataaaacaga tctcctggct
      301 gtccttttta tgtgcaacca tcagtccgat gtcttccgca atcgcacgga tctcatcctt
      361 tgtgtaatct cctaccggca tgagggtatg ggagagctgc ttctgcgtga gattaaacag
      421 cgcgtaggtc tgatcctttg ccgccgtcac ggagttgcgg atagcatatc ttccgttgga
      481 aagctgttcc acgcgcgcgt agtggccggt cgcaatgtaa tcggctccga tctccaaact
      541 cctttgaagc agggattccc atttcacata gcggttgcat gcaatacacg gattcggcgt
      601 gcgccctttt aaatactccg ctgtaaaata atccatgaca ttcttcttga actcgcgctt
      661 gaaattcatc acataatacg gaatctccag acgatccgcc accctgcgtg cgtcatccac
      721 cgccgagagt ccacagcatc cgccgctctc cgcctgtgtg aactgatctt catcctgcca
      781 gatctgcatt gtgactccga tcacatcata tccctgctgc tttaacagat atgccgcaac
      841 ggaagaatcc actccgcccg acataccgac tactactttt tttcctgcca tgcccgctcc
      901 tgccaacaaa catatgaggt tgccacacct tacggtatgg cagcctcatc tcttcgtgtc
      961 ttccaatgtt aaattccgga attgtaaaga tctccggtgc tgtaattata agtatacata
     1021 ccattcttgc tgtaggtatt cgccttcgcc gcttcactct tcgcgttgga tgcgatcttc
     1081 aggagatcca cattcgtctg cgtcgcataa gatccgtgcg ttgtaaacag tgacttcagc
     1141 ttgtccatat ccgccttctc aaagctcttc tcgtccacgg aaagtctgcc gtcggcatcc
     1201 acggtaatac ccgctttctc caacatcttc tcattcgctc tggcgttgct cttcaaagag
     1261 ttcgccgcac gcagaatgct cttcgtctta gaatcgtccg cccgatccag cagtgaattg
     1321 taatcatcca catacgactt caccgcctta tagatggcat cacggtcata ctcctgcgtc
     1381 ttcgtgccgt cctccgcggt cttctccact ttgttgaaca cagaatcctt tccggttgct
     1441 gtcagcttgg aaagcgactt ctccgtactc tccgccgcac tctcaatccg ggtcagtgtc
     1501 ttcgcgtcat ccttcgcggt tgagatcgac gggctgacct tcgtggcgct gtccgtggaa
     1561 tccgacttgc tgtaataggc actcaaaagc ttgaaatagc ttccgttgcg gatcgtcgca
     1621 taatcggaat aattgattcc gagcagatcg cttccgcctc ccagcagaga ggaagttgta
     1681 ttgttccggt tcagactgga aaataacacg cccagtgacg atgaatcata tcctgaaata
     1741 cttgccatac tctatcactc ctttgaaaaa aagcatctga cctccgtgtc agaatgttga
     1801 tccggctgtt gcatttgtgc acaatacctt cttgttacct catgttctat atcgtcattt
     1861 tagcccgaaa ccttagattt ttcaagcatt ttctggtgct tgtccttatt aaaatgttta
     1921 actattgttc atagtttatt catatcttat tgctacaata aaattatcaa aaaacaagac
     1981 aattcgttac aatatatttg ataatttttt cataatagcc caggcgcgca agtgtctggg
     2041 cactcctcat ttcagggggt ttttctccct tttacgcaga cagagagggg cgccgcacat
     2101 gcgcggcacc cctctcttct attccagatc catccgttct gtattatttc tgatcattat
     2161 acttcagatc acgcacatca taaccgtggc gctctaacgc gcgtatcaca cgctcaaaat
     2221 ccggtgcttc cacccgaagg aagatctctg ccagatccat aacatccgtc ggaatcacca
     2281 tcatgttgca gatgtctccg ccctccttag caatcagctc acagatcttg ccgagcacac
     2341 cgcggatatc atacgtgtag attgccatgg aattgtgttt gttaccaaag atcttctgat
     2401 attccttgaa taccgcctgc tgcgtcacga ttccaagcag cttgttctgc tcgttcgtga
     2461 tcggaataaa acgaactttg gaggtgataa acattgctgc cgcttcctca atccgtgtat
     2521 tctcaccgat ggtctcaatt cttgtcttca tgaactccat cacttttcgc tgtgcaaatt
     2581 cttctctggt ccctgtatag ttccggaaaa attcctcaaa caaaaactgt ttggacaaaa
     2641 cacccacaaa ctgctgtccg tccactaccg gaagcgagag aagtccctgc tcgtcgataa
     2701 tcttcattgc ctcttctacc gtattctcca cgctgataca cgccagctta ctgaaaggca
     2761 tcataattgc ttttacacgc ataaccaata cctcctgtct gcttttattg taagtatatt
     2821 atacactaaa tattctcaat ttgccagtct atcggtgcga ttccatgctc ctcaagaaac
     2881 gcatttgcct tgctgaaatg ccggcacccg aaaaatcccc ggtatgccga gagagggctc
     2941 ggatgcggtg ccgttaagat cagatgcttc ggattcgtaa gcatcggtat tttggactgc
     3001 gccggtctgc cccagaggag gtacacaatc ggacgatcct gggcatttac tgcctgaatg
     3061 accgcatcag tgaactgctc ccagcctctt ccctgatggg aattcgcctg gtgcgcgcgc
     3121 acggtgagga cggtgttgag cagaagcact ccctgatctg cccatttttt cagataaccg
     3181 ttattcggaa tgtaacagcc cagatcatca tgcagttcct tataaatgtt ctgcagggag
     3241 ggcgggattt ccttttgctc cggcagcacg gaaaacgaaa gaccatgtgc ctgatttaca
     3301 ttgtgatagg ggtcctgccc caacagaagc accttcacct cactaagcgg cgtaaaatga
     3361 aaggcattaa agatatcctc cgccggcggg tagaccactg ttctgctgta ttcctcccgc
     3421 acaaatgtgt ataattcccg gtaatacggc tttcggaatt ccccctggat tgccggaagc
     3481 cagtcattgt caatcatcga catatcccac tactcctttt ttcacagaca gcagtgtggg
     3541 cgcaccgtgc tatcgccgca cagatacgcc cctcgctgcc agatcgttct tcagatccat
     3601 aatctcaagt tctttatagt gaaacaggga tg
//
LOCUS       DBMDQJ010000190         3619 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9428_length_3619_cov_1.300171, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000190 DBMDQJ010000000
VERSION     DBMDQJ010000190.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9428_length_3619_cov_1.300171"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..463
                     /locus_tag="ACZO9R_05105"
     CDS             29..463
                     /locus_tag="ACZO9R_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912476.1"
                     /translation="METNEHIEALLKEPYCIIDILPKRVPCESMGQYFAVERFFLAPE
                     RLREIRKKQTELLLKLNCYYDMRASFDAGETWSMNPDPEWLALELASLSNGKAVRILF
                     PEQETMIDLDGCDTYMTVYHPSDEMCDLLGRIAGAEGLFLWE"
     gene            complement(476..949)
                     /locus_tag="ACZO9R_05110"
     CDS             complement(476..949)
                     /locus_tag="ACZO9R_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912477.1"
                     /translation="MKPRSIRESFRTFPAVLQHQILLRCGLTLGGLAAFGLFWALSSA
                     FWFGAPFLMLAFWMGVSAVQTFRTVFHGKYARVHAVCKQVISTAILKRPKALILSTEQ
                     GNLRLRIQQRIRKPEVGDVFTVYLADSAMLYESEEEYVVSSYLGITRAVDKAGTA"
     gene            complement(946..1503)
                     /locus_tag="ACZO9R_05115"
     CDS             complement(946..1503)
                     /locus_tag="ACZO9R_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912478.1"
                     /translation="MYYISKGIPEKGGITAQLRIVTGGQIFTLFGLDETLWLRARFEV
                     VATATPEEEAVRKMLRLGLMEYTSMEDPAARYTLLSRCMIVPAKPSKRNALRRDEKIL
                     LDWMLGAGFRLSLAELVYLFDNRVRPTKDLLGKENMQALVDRIYPDQKIEDHVLEAKM
                     ADAAQRDAVVDAVLGLLKKKRVVLL"
     gene            complement(1930..1992)
                     /locus_tag="ACZO9R_05120"
                     /pseudo
     CDS             complement(1930..1992)
                     /locus_tag="ACZO9R_05120"
                     /inference="COORDINATES: protein motif:HMM:NF024645.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2158..>2361)
                     /locus_tag="ACZO9R_05125"
     CDS             complement(2158..>2361)
                     /locus_tag="ACZO9R_05125"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0912479.1"
                     /translation="LREKKGLSQDVVSGFAGIGRTHLSAIERGLRKPTLETFVRVCEA
                     LGVKPSDLMALIEQAGAIKDDPD"
     assembly_gap    2362..2394
                     /estimated_length=33
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2573..2884
                     /locus_tag="ACZO9R_05130"
     CDS             2573..2884
                     /locus_tag="ACZO9R_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912480.1"
                     /translation="MKGKAWFVFEPRRTDDLAFGRCEGKWMEFEVVKTICLDKIDYEN
                     FSTDLLADRQFIEDNIALCEKPGDCLLVTNRRHTEEILVIPWHGCFVRYAALRPPVQL
                     L"
BASE COUNT          841 a          968 c          923 g          854 t
ORIGIN      
        1 cggcaatcaa atgacggaga tatctgtgat ggaaacaaat gaacatattg aggcgcttct
       61 gaaggagccc tactgtatta ttgacattct accgaagcgg gttccctgcg aatctatggg
      121 acagtatttt gccgtagagc gattcttcct tgccccggaa cggcttcggg agattcggaa
      181 aaagcagacg gagctgcttc tcaagctgaa ctgctattac gacatgcgtg catccttcga
      241 cgcaggggaa acctggtcta tgaaccccga tccggaatgg cttgcgctgg agctcgcctc
      301 cctgtcgaat ggaaaagctg tccgcatcct tttcccggag caggagacca tgattgatct
      361 ggacggttgc gatacctata tgacggtcta ccatccgtct gacgagatgt gcgatcttct
      421 ggggagaatt gcaggggcgg aaggcctgtt tctgtgggaa tgacaagtcg gagattcagg
      481 cagtacctgc tttgtcaacg gccctcgtaa ttcccagata actgcttacg acatactctt
      541 cctcgctctc gtagagcatc gcgctgtccg cgagatagac ggtaaataca tcaccgacct
      601 ccggttttcg gatccgctgc tggattcgga gccggaggtt tccttgttct gtggagagga
      661 tcagcgcctt gggacgtttc aaaattgccg tggagatgac ctgcttgcag acggcatgga
      721 cacgggcgta tttcccgtga aacaccgtcc ggaatgtctg gacagcagag acgcccatcc
      781 agaatgccag cattagaaac ggcgcaccga accagaacgc cgaggatagc gcccagaaca
      841 gcccgaaggc cgccagcccg cccagcgtca agccgcagcg cagcaggatc tggtgctgga
      901 ggactgcggg gaaggtccgg aagctctccc ggatcgatct cggtttcaca gcagcaccac
      961 ccgcttcttt ttcagcagac ccaggactgc gtcgacgacg gcatctcgct gcgctgcgtc
     1021 agccatcttt gcctccagga catggtcttc aatcttctga tccggataga tgcggtctac
     1081 gagagcctgc atgttctcct tgcccaggag gtccttggtt ggccgaactc ggttgtcaaa
     1141 cagatatacc agttctgcca gactcagacg gaagcccgct ccgagcatcc aatccaggag
     1201 aatcttttca tcccggcgca gggcatttcg cttgctcggt ttggcgggga cgatcatgca
     1261 gcggctgagt agggtgtagc gcgcggccgg gtcctccatc gaggtgtact ccatcagacc
     1321 gagtctgagc atcttgcgga ctgcttcctc ctccggcgtt gccgttgcga ccacttcaaa
     1381 tctggctcgg agccagagag tttcgtccag accgaacagc gtgaagatct gcccgccggt
     1441 cacaatgcgg agctgagcgg tgatgccgcc cttttcgggg atgcctttgg aaatgtagta
     1501 catagtcaat cactcctgtt tcattacacg gttttaggtc acggattaca acacggtcat
     1561 cagcaccacg cagagggcga tggtcagcaa ggcgcctgca accagaagca cgatcttctt
     1621 gatcatctcc cgatccagac ccggtctgac aacatcggta tccaatggcg cgacatcgtc
     1681 gtagtaccca tcgtaatcgt ccggcaaggc gccttccggt gcagcatcct ccccagaccg
     1741 agatgagagc ttctccgtgg gtgctccttc gatcgatgat ccgggattct ttcgcggctt
     1801 ccgtctcggt ctggcgggag ccgccttttt gcgtgtttcc cgagggcgag tatctttcct
     1861 cgagggtctc tccagggcgg tctctgtctc agtctgacga gagcttccga ccggcagagc
     1921 ttttccacac tctgcgcaga acgagacctc cggactcagc agatccgcac cgcagaacgg
     1981 gcagtatttc atttgcacga tcctccttct tcaaaaatgg cataagaaaa cacgcgagcc
     2041 cggatggacc cacgtgttca cagtaggagt atatcacatt atttctgcca tgtcattggc
     2101 aaaactctgc caaatctctg ccatttttgt gccatttcat tttcagcccg gaattgtcta
     2161 gtccgggtcg tcttttattg cacccgcttg ttcgatcaag gccatcagat cactcggctt
     2221 gacgccaagc gcttcgcaaa cgcgcacaaa ggtttccagg gtcggtttcc gaaggccacg
     2281 ctctatcgca ctgagatgcg tcctaccgat gcccgcaaag ccgctgacaa catcctgcga
     2341 cagccctttc ttctccctga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgaca
     2401 gcaactgcat ccagcattta tctgcgcctc cttacacgca aattctactg gaagatcaga
     2461 gtttttctga atacatatgt tgtcatttga acgcaaatgt gttactttta tcggagaaac
     2521 accagaaaaa gatcagcggc aatcccataa ttgccgtcag gatggaacat agatgaaagg
     2581 aaaagcatgg ttcgtatttg agccgagacg cacggacgat ctggcctttg gccgctgcga
     2641 ggggaagtgg atggaatttg aagtcgtaaa aacaatctgc ctggataaga tcgactatga
     2701 gaactttagc acagatctgc tagccgaccg gcagttcatc gaggacaata tcgccctgtg
     2761 cgagaaaccc ggagattgcc tcctggtcac caaccgccgc catacggagg agatcctcgt
     2821 cattccctgg cacggctgct tcgtgcgcta tgctgcattg cgtccgccgg tgcagctgct
     2881 gtgacagaat cacggggttt cagcaccacc atcacggagt ttcagcgcca ccctctcggg
     2941 gttttagcac cggtggcacg cagcaacagc accactcgtt accgatgccg catgcctggc
     3001 tgaaaagaga tatacgataa tatattatgg taaactatga ccgcctataa ttgtgccgga
     3061 ttgcctgatc tcaggcttcc ggcacatttt cttccgaaag gcaaacatat ttggcgttgt
     3121 catcatcgcc gaagtgcctg gcggcaaagg cggatcgtca gccttgaaag gcccgcactt
     3181 ctctcaaccg ctctgcgagc ttcgtatttc taagctgcgg tttgtcatag aggttacgtt
     3241 taattgggtt gatggcaact tctccgaaca tatgaaatac tgtacttttt tgcatttctg
     3301 tgataaaatg caaaatggaa actggtggca tttctgcttt tcagcagctt ccacgaaaca
     3361 tgtttaatca gcaggttttt tcaatagatg aatagatgat tggtataacc gtaagccaaa
     3421 tagcaagata aaaactgaag cacagtttac tattccatag taactggaca ttgagaattt
     3481 gttaatcctg catgattacc aatgttcggg cccggcggaa atcgccaaat ggtcatgtca
     3541 gttgggcgct aacaccaaga ttaagactta agaaaaacag gtgaaaaaat ggaatacgta
     3601 attacaaaaa taaatggct
//
LOCUS       DBMDQJ010000191         3600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9482_length_3600_cov_1.759360, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000191 DBMDQJ010000000
VERSION     DBMDQJ010000191.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9482_length_3600_cov_1.759360"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..850
                     /gene="trpB"
                     /locus_tag="ACZO9R_05135"
     CDS             <1..850
                     /gene="trpB"
                     /locus_tag="ACZO9R_05135"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR0912481.1"
                     /translation="ALFDMDCTVYMGAEDIKRQSLNVMRMEMLGAKVVSVTSGSNTLK
                     DATNEAIRTWAKTAEDTFYIIGSAVGPYPYPKMVKEFQSVIGREAKKQILAAEGKLPD
                     VVMACVGGGSNSIGMFADFIEEKGVKLIGVEAAGKGIETGEHASAMALGEPGVLHGMR
                     SYLLQDADGNVQLAHSISAGLDYPGVGPEHAYLKECGRARYVAIRDDEAMDALMELCR
                     LEGIIPAIESAHAVAYAMKYARERKEAVGEKQASEETMVICLSGRGDKDVNTIADYLA
                     GKGYLN"
     gene            888..1679
                     /gene="trpA"
                     /locus_tag="ACZO9R_05140"
     CDS             888..1679
                     /gene="trpA"
                     /locus_tag="ACZO9R_05140"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR0912482.1"
                     /translation="MNRIEARMNACSKKQEKAFITYITAGLPDLAATKEIIRAQERGG
                     CDVIELGVPFSDPLADGPVIQDASYRAICGGVNVKKIFAMMQELRTEGVTVPIIFMMY
                     YNTILHYGVEAFVKRCNEAGVDGLIVPDLPFEEQEELMTALDASEDTILIQLVSPVSG
                     ARIPKILAGARGFVYCVSSMGVTGQGAEFHKEVRSYLTSVREATDLPVMMGFGIRTAA
                     DVLPLADLIDGAIVGSYFIRLLEEQGFAPEAAETYCRTFKKELNA"
     gene            1694..2704
                     /gene="trpD"
                     /locus_tag="ACZO9R_05145"
     CDS             1694..2704
                     /gene="trpD"
                     /locus_tag="ACZO9R_05145"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR0912483.1"
                     /translation="MKEYIAKVAEGENLTEEEAKGAMDIMLSGDATQAQIAAFLALER
                     VKGETLDELAGFAVVLRDKAETISPKVDNYIDLVGTGGDRTFTFNVSTTSAFVVAAAG
                     LPVAKHGNRSISSKSGAGDVLEALGVNIMADPKKVEECVEKTGIGFMFAQLFNKSMRY
                     VGQARSEMGIRTVFNILGPLANPSRAKNMVVGVYSPSLTEKVATAMSRLGVERAYVVS
                     GEDNMDEITLTGATTVSEIRDGAVTTFTITPEQFGMKRVSLEELRGGSGGENAKITRA
                     ILDGTERGAKRDIVLLNAGATLYVGGVAESMEAGVKLAAEAIDSGAAAQKLEELVRVS
                     NS"
     gene            2723..>3600
                     /locus_tag="ACZO9R_05150"
     CDS             2723..>3600
                     /locus_tag="ACZO9R_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR0912484.1"
                     /translation="MIRFNSDYTEGCHPAILERLAATNMEQTPGYGEDAYCAAAAEQI
                     KEACAAPEAAVHFLVGGTQTNVTVISAALRHYQGVITAVTGHINVHETGALESCGHKC
                     LTIETADGKLTAEQIAAYTDAHYADASFEHTVQPKMVYISNPTEIGTIYKKAELEAIY
                     DVCKERGLYLFLDGARLGYGLTCRENDLTLADVAALTDVFYIGGTKVGALFGEAVVIT
                     NEELKRDFRYNMKQRGGMLAKGRLLGIQFLTLFEENRYFEISAHASRLAEKIHDELKD
                     MGVKFYVDSPSNQQFV"
BASE COUNT          895 a          786 c         1197 g          722 t
ORIGIN      
        1 ggcacttttt gatatggatt gtacggtcta tatgggagcc gaggatatca aaagacagtc
       61 gttaaacgtg atgcgcatgg agatgctcgg cgcgaaggtt gtcagtgtca catcgggaag
      121 caatacacta aaggatgcga ccaacgaggc gatccgtacc tgggcaaaga cggcggagga
      181 taccttttac atcatcggtt ctgccgtggg accgtatccg tatccgaaga tggtaaaaga
      241 gtttcagagc gtcatcggcc gcgaggcgaa aaaacagatc cttgcggcgg agggaaagct
      301 tccggatgtc gtgatggcat gcgtgggcgg cggatcgaac tccatcggca tgtttgcgga
      361 ttttattgag gagaaggggg taaagctgat cggcgtggag gcagccggaa agggaatcga
      421 gaccggggaa cacgcgtcgg cgatggcgct cggcgagccg ggagttctgc acggcatgcg
      481 ttcctatctg ctgcaggatg cggacggcaa tgtccagctt gcgcattcca tctcagcagg
      541 gctggattat ccgggcgtcg gtccggagca cgcgtattta aaggagtgcg gacgcgcacg
      601 atatgtggca atccgggatg atgaggcgat ggatgcgctg atggagctgt gcagactgga
      661 gggaatcatt cctgcgatcg agagcgccca tgcagtcgcc tatgcgatga agtacgcgag
      721 ggagcgcaaa gaggcggtcg gtgagaagca ggcgtcagag gagaccatgg tcatctgtct
      781 gtcgggccgc ggcgacaagg atgtcaatac gatcgcagat tatcttgcag gaaaaggata
      841 tttaaattaa gccgggaagt tgtccggcga gggaggatat gacagatatg aatcgaatag
      901 aagcgcgtat gaacgcctgc agcaaaaagc aggagaaagc atttattact tatattaccg
      961 cgggactgcc ggatctggca gccacaaaag aaatcatccg ggcgcaggag cgcggcggct
     1021 gcgacgtgat tgagcttggc gtgccgtttt ccgatccgct ggcggacgga ccggtcatcc
     1081 aggacgcatc gtaccgtgca atctgcggcg gggtgaatgt gaagaagatt tttgcaatga
     1141 tgcaggaact gcgcacggag ggtgtgacgg tcccgatcat cttcatgatg tattacaaca
     1201 cgatcctgca ctacggcgtg gaagcgtttg taaaaagatg caatgaggcg ggggtggacg
     1261 gactgatcgt tccggatctg ccgtttgagg agcaggagga acttatgaca gcgctagatg
     1321 catcggagga tacgatctta atccagctcg tgtcgccagt gtccggggcg cggattccaa
     1381 agattctcgc cggagcgcgg gggttcgtct actgtgtatc gtccatgggt gtcacggggc
     1441 agggggcaga gttccataag gaggtaagaa gctatcttac ctcggtacgg gaagcgacgg
     1501 atctgccggt gatgatgggc tttggcatcc ggacggcggc ggatgtcctg ccgcttgcgg
     1561 atctgatcga cggagcgatc gtcggaagtt attttatcag gctgttggag gagcagggct
     1621 ttgcgccgga ggcggcggag acatactgcc ggacctttaa aaaggaactg aacgcataga
     1681 gggggaaaaa gggatgaaag aatatattgc aaaggttgcc gagggagaga atctgactga
     1741 ggaagaagca aaaggggcga tggacatcat gttaagcggt gatgcgacgc aggcgcagat
     1801 cgcggcgttt ctggcgctgg agcgcgtgaa gggcgagaca ctggatgaac tggcaggatt
     1861 tgcggtggtg ctccgcgaca aggctgagac gatttccccg aaggttgata attatatcga
     1921 tctggtggga accggcggag accgcacatt tacctttaac gtgtccacta cgtcggcgtt
     1981 cgtggtggct gcggcgggac ttccggtggc aaaacacgga aaccgctcca tctcgtcgaa
     2041 atcgggcgcc ggggatgtgt tagaggcgct cggcgtcaat atcatggcag acccgaagaa
     2101 agtggaggag tgtgtggaga agaccggaat cggtttcatg tttgcgcagc tttttaacaa
     2161 gtccatgcgc tatgtcgggc aggcgcgcag cgagatggga atccgtaccg tctttaatat
     2221 cttaggaccg ctcgctaatc cgtcccgcgc aaaaaatatg gtggtcggtg tctacagtcc
     2281 gtcactcacg gaaaaggtag cgacggcgat gagcagactt ggggtggagc gcgcgtatgt
     2341 agtcagcggt gaggacaaca tggatgagat tacgctgaca ggagcgacca cggtatccga
     2401 gatccgagat ggagcggtga caacatttac catcacaccg gagcagttcg gtatgaagcg
     2461 cgtatcgctg gaggagctgc gtggcggaag cggcggggaa aacgcaaaga ttacgagagc
     2521 cattctggac ggaacggagc gcggcgccaa gcgtgacatc gttcttttga acgcaggtgc
     2581 aactctttat gtcggagggg ttgcggaatc tatggaagcg ggtgtaaaat tagcggcaga
     2641 ggcgatcgac agtggcgcag cggcacagaa gctggaggag ctggttcgcg tctcaaattc
     2701 atagagaaaa ggaatcagag agatgatacg gtttaacagt gattacacag aaggatgcca
     2761 tccggcgatc ttagagagac ttgcggcaac caacatggaa cagaccccgg gatatgggga
     2821 ggatgcatat tgtgcggcag ctgcggagca gataaaagag gcgtgcgccg caccggaggc
     2881 agcggtgcat tttctggtgg gaggaacaca gacgaatgtg acagtgattt cggctgcatt
     2941 aagacactat cagggtgtga tcactgcggt gaccggacac atcaacgtac atgagacggg
     3001 agcactggag tcctgcggac acaagtgcct tacgatcgag acggcggacg gcaaactgac
     3061 agcagaacag attgccgcat atacggacgc gcattatgcc gacgcaagtt ttgaacacac
     3121 ggttcagccg aagatggtct atatttccaa tccgacggag attggcacga tctataaaaa
     3181 agcggagctg gaggcgatct atgatgtctg caaagagaga gggctttatc tgtttttaga
     3241 cggtgcaaga ctgggctacg gtcttacatg ccgggaaaat gatctgacac ttgccgatgt
     3301 ggcggcactc acggatgtct tctacatcgg aggaaccaag gtcggagcgc tctttggcga
     3361 ggcggttgtg atcacaaacg aggaattgaa gcgcgatttc cgttacaata tgaagcagcg
     3421 gggcggcatg ctggcaaaag gcagactgct cggcatccag ttcctcacgc tattcgagga
     3481 gaaccggtat tttgagatct cggcgcacgc atcaaggctt gcggaaaaga tccatgatga
     3541 gctaaaggac atgggcgtaa aattctatgt ggactcgcca tcgaaccagc agtttgtgat
//
LOCUS       DBMDQJ010000192         3594 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9504_length_3594_cov_1.255940, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000192 DBMDQJ010000000
VERSION     DBMDQJ010000192.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3594)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3594)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3594
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9504_length_3594_cov_1.255940"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            81..545
                     /locus_tag="ACZO9R_05155"
     CDS             81..545
                     /locus_tag="ACZO9R_05155"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR0912485.1"
                     /translation="MSEKEIEVYGKQVDEIKIRPYEHHAKYYETDQMGIIHHSNYIKW
                     MEEARMDLMDQIGLSYKEMEAMEIISPVLSVSCEYHSMVHFDDVVVIEPRITKYNGIK
                     MEVEYRMTDKATGELRTTGTSSHCFLNRSGRPISLKRSYPEIDTKFFEYTDI"
     gene            587..2377
                     /locus_tag="ACZO9R_05160"
     CDS             587..2377
                     /locus_tag="ACZO9R_05160"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HIR0912486.1"
                     /translation="MIQERLSALREEMKKRKIDIYIVPTADFHESEYVGEHFKARKFI
                     TGFTGSAGTAVITLTEAGLWTDGRYFVQAEKQLAGSTVTLYRMGEEGVPTVDEFVEKE
                     LPESGCIGFDGRVVNGSWGRKLLAIAEKKKGSLYVTEDLIDLIWKDRPALSKEPLFLL
                     EEKYSGKSTADKIADLRKVMEKEGANVHILTSLYDIAWLLNIRGGDIDYVPVILSYLV
                     LTDKECIWFLQEEVVDEKIAAYLKENHITTRPYDAIYDYVKEIPADACVLMNGNTVNY
                     RITSSLRKEIRIVDQPNPTEIMKAVKNPVEVENIRKAHVKDGVAFTKFMYWLKTNIGK
                     IPMTEISASDYLEARRREQDNFIELSFDTICAYGPNAAMMHYAATPESDAELKPEGFL
                     LVDSGGHYFEGTTDITRTMALGPITDEMRLHFTTVCRSNMNLANAKFLYGCTGLNLDI
                     LSRGPLWQMGIDYKCGTGHGVGYILNVHEGPNGFRWRVVPERHDNGTLEEGMVTTDEP
                     GVYLEGKYGIRTENELVCHKAEKNEYGQFMNFENITYAPIDLDAIDPDEMTGREKKML
                     NDYHAMVYKTLSPYMTPEENEWLKKYTRAI"
     gene            2392..>3594
                     /locus_tag="ACZO9R_05165"
     CDS             2392..>3594
                     /locus_tag="ACZO9R_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009261974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HIR0912487.1"
                     /translation="MSEKLVLIDGHSILNRAFFGIPDLTNSEGLHTNAVYGFLNIMFK
                     ILEEEQPDYLTVAFDVSEPTFRHKMFAEYKGTRKPMAAELKQQVPLMKEMLRAMGVTI
                     VEKGGYEADDLLGTIAKRSEAAGVEVAVVSGDRDLLQLATDHIKIRIPKTKRTGTEIE
                     DYNAKEVLEKYQVTPTEFIDVKALMGDTSDNIPGVPGIGEKTATAIIAKYGSIENAYA
                     HVDELKPPRASTNLKEHYDMAQLSKTLATIKIDADIPYDISEARIGNLYTPEAYVMCK
                     RLEFKNLLKRFEVDTPVNTGEENFKRIEDFAGVEDFFAKAKKQQAAGVQLIYEKDVVL
                     GLAVACTKEDVAYIPVAGFVTEQYLFEQSRQLLAAGTEIRTFDLKPQLKWIEADEKSH
                     IFDVKIAAY"
BASE COUNT         1040 a          730 c         1037 g          787 t
ORIGIN      
        1 gggatgtggg ataatgtacg cgtaggcaat gagccgggcg aagccattgt ctatgtattg
       61 taaggaaaga aggaattcag atgtcagaga aggaaatcga agtctatgga aaacaggtgg
      121 atgagatcaa gattcgtcct tatgagcatc atgccaagta ttacgagacg gatcagatgg
      181 gcatcattca tcattccaac tatatcaaat ggatggaaga ggcgcggatg gatctgatgg
      241 atcagatcgg actctcctat aaggagatgg aggcaatgga gatcatcagc ccggtgctgt
      301 cggtgtcctg cgagtatcac agcatggttc attttgatga tgtggttgtc attgagccgc
      361 ggattaccaa gtataacgga atcaagatgg aagtggagta ccgaatgacg gataaggcga
      421 cgggagaact tcgcactaca ggaacaagtt cccactgctt tttgaaccgt tccggaagac
      481 cgatttcgtt gaaacggtct tatccggaga ttgatacaaa gttctttgag tacacagata
      541 tttagaacaa aatggagcgg atagacgctc cggaatggag aatactatga ttcaggaaag
      601 attaagcgca ttgcgcgagg agatgaaaaa gcggaagatc gatatttata ttgtaccgac
      661 cgccgatttt catgagtccg aatatgtagg ggagcatttt aaagccagaa agtttatcac
      721 cggtttcacc ggatcggcag gcacggcggt cattaccctg acagaggcag gactgtggac
      781 agacggacgc tatttcgtgc aggcggaaaa gcagcttgcc ggaagtaccg tgacgcttta
      841 ccgcatgggt gaagagggtg tgccgaccgt ggatgagttc gttgaaaaag aactgccgga
      901 gagcggctgc atcggatttg acggcagagt ggtgaacgga agctggggca gaaagcttct
      961 cgccattgcg gagaaaaaga agggttcttt gtacgtgacg gaggatctga ttgatctgat
     1021 ctggaaggat cgcccggcgc tttctaagga accgctgttt ttgcttgagg agaagtactc
     1081 cggcaagagc acggcagaca agatcgcgga tctgcgcaag gtaatggaaa aagagggagc
     1141 caatgtgcat attctgactt ccttatacga tatagcatgg ctgttaaaca tccgcggcgg
     1201 agacatcgat tacgtgccgg tcatcctgtc ctatctggtg cttaccgaca aggagtgcat
     1261 ctggttttta caggaagaag ttgtggatga gaagattgca gcatatctga aagagaatca
     1321 catcacaacc agaccttacg atgcaatcta tgattatgtg aaagagatcc cggcggacgc
     1381 atgcgtactg atgaatggca atacggtcaa ctaccgcatt accagcagcc tgcgaaagga
     1441 gatccgcatt gtggaccagc cgaacccgac cgagatcatg aaagcggtca agaatccggt
     1501 cgaggtggag aatatccgca aggcacatgt gaaggacggt gtggcattca ccaaattcat
     1561 gtactggctg aagaccaata tcggcaagat tccgatgacg gagatctccg catcggatta
     1621 cctggaggcg cgcagaagag agcaggataa ctttatcgag ttaagctttg acacgatctg
     1681 tgcatacgga ccgaacgctg ccatgatgca ctatgcggcg actccggaat cggatgccga
     1741 gttaaagccg gaaggcttcc tgctggtgga ttccggcgga cactatttcg agggaacgac
     1801 cgacatcaca agaacgatgg cgctcggacc gatcacagac gagatgcggc tgcattttac
     1861 aacggtatgc cgctccaaca tgaaccttgc aaacgccaag ttcctctacg gctgcaccgg
     1921 actgaatctg gatattttat cccgcggacc gctctggcag atgggcattg attacaagtg
     1981 cggtaccgga cacggtgtgg gctacatttt gaatgtgcac gagggaccga acggcttccg
     2041 ctggagagtg gtgccggagc gccatgataa cggcacgctg gaagagggta tggtcacgac
     2101 ggatgagccg ggcgtatatc tggaaggcaa atacggtatc cgtaccgaga acgaactggt
     2161 atgccacaag gcggagaaaa acgagtacgg ccagttcatg aattttgaaa atattacgta
     2221 cgcaccgatt gatctggatg ccattgatcc ggacgagatg acgggacgtg agaagaagat
     2281 gttaaatgac tatcatgcaa tggtatacaa gacactctct ccgtacatga caccggaaga
     2341 gaatgagtgg ctgaagaagt atacaagagc aatctagaaa cggagacaga aatgagcgaa
     2401 aagctggtat tgattgacgg acacagtatt ttgaaccggg catttttcgg aatcccggat
     2461 ctgaccaatt ccgagggact tcacacgaac gcggtatacg gatttttaaa tatcatgttc
     2521 aagatactgg aggaggagca gcccgattac ctgacggttg cgttcgacgt gtctgagccg
     2581 acgtttcgcc acaagatgtt tgcggaatac aagggtaccc gcaagccgat ggcggcagag
     2641 ttaaagcagc aggtacccct gatgaaagag atgctgcgcg cgatgggtgt gaccatcgtt
     2701 gaaaaaggcg gttacgaggc ggatgatctg cttggaacca tcgcaaagcg cagtgaggcg
     2761 gcaggcgtgg aggttgcggt cgtatccggt gaccgtgatc ttctgcagct tgcaacagat
     2821 catatcaaga tccggatccc gaagacgaag aggacgggaa ccgagattga ggactacaac
     2881 gcgaaggaag tcctcgagaa gtatcaggtg acgccgacgg agtttattga tgtgaaggca
     2941 ctgatgggtg atacttccga caacattccg ggagttcccg gcattggcga gaagacagcg
     3001 acggcgatca tcgcaaaata cggcagtatt gagaatgcct acgcacatgt ggatgaatta
     3061 aagccgccga gagcaagcac gaatttaaaa gagcattatg atatggcaca gttgagtaag
     3121 acactggcta ccattaagat agacgcagat atcccatatg atatcagtga agcacgaatt
     3181 ggtaatttat atacaccgga agcttatgtg atgtgtaagc gtctggaatt taagaatctt
     3241 ctgaaacggt ttgaagtgga cacaccggta aataccggag aggaaaactt taaacgaatc
     3301 gaggattttg ctggagtaga agattttttt gcaaaggcaa agaagcagca ggcagcaggt
     3361 gtccagttga tttatgaaaa ggatgttgtg cttggacttg cagttgcatg cacaaaagaa
     3421 gatgtggcat atattccggt tgcgggattt gtgacagagc agtatctatt cgaacagagc
     3481 aggcagcttc ttgcagcagg cacagagatt cgcacctttg atttaaaacc gcagcttaaa
     3541 tggatagaag ccgatgaaaa aagtcatatt tttgatgtga aaatagcagc ttat
//
LOCUS       DBMDQJ010000193         3585 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9528_length_3585_cov_1.816016, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000193 DBMDQJ010000000
VERSION     DBMDQJ010000193.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9528_length_3585_cov_1.816016"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1569)
                     /locus_tag="ACZO9R_05170"
     CDS             complement(<1..1569)
                     /locus_tag="ACZO9R_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HIR0912488.1"
                     /translation="MEKYRETDLRYLKSLANQYPTVAAAATEIINLQAILSLPKGTEH
                     FITDIHGEYDQFQHVIRNGSGAIKRKIEEEFGNAISAGEKKAIATLIYYPEQKLEQVL
                     KTEENMEDWYKVSLYRLIRICKSASSKYTRSKVRKALPKDFAYVIEELLTGRPDVSDQ
                     EAYYNEIIRSVIRTGRAPELVIAFCNLIRRLVVDHLHVVGDIFDRGPYPNLIMDTLMQ
                     HHSVDIQWGNHDIYWMGAAAGSRPCICNAIRISAKYGNLNLLEDGYGINLVPLARLAM
                     SRYDKDPCICFKLDYREDEYDVRDAMLDEKMHKAITVIQFKLEGQMIMRHPEFGMDER
                     LLLDKINIEKGTVCVEGKEYPMKDLNFPTIDWKHPYELSSEEEDVMERITQAFLNCEK
                     LQRHVRFLFTQGSLYKVYNGNLLYHGCVPMNEDGTFTRVNVYGKEYSGKALYDVLENY
                     ARKGYYAIDPGEKKKGLDILWFIWENQNSPVFGKAKMTTFERYFIADKATHQEPKNPY
                     YRLLEEEEVVNRILS"
     gene            complement(1758..2534)
                     /gene="dapB"
                     /locus_tag="ACZO9R_05175"
     CDS             complement(1758..2534)
                     /gene="dapB"
                     /locus_tag="ACZO9R_05175"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR0912489.1"
                     /translation="MIKAIMNGCNGKMGQVISAICKEDPQIEIVAGVDLYDGIKNDYP
                     VFPNISVCDVKADVIIDFSNAKAVDDLLVYSVDKKIPVVLCTTGLSEEQMEQVKAASE
                     QVAVLKSANMSLGINLLMQLLAQAATILAPAGFDMEIVEKHHNQKLDAPSGTALALAD
                     SMNDALGHVYEYKYDRSSERKKRDPHEIGISAVRGGSIVGEHEVIFAGLDEVIEFKHT
                     AYSRSVFGKGAVEAAKFLAGKKAGFYDMQDVIRATMEQAD"
     gene            complement(2654..3544)
                     /gene="dapA"
                     /locus_tag="ACZO9R_05180"
     CDS             complement(2654..3544)
                     /gene="dapA"
                     /locus_tag="ACZO9R_05180"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR0912490.1"
                     /translation="MAIFKGAGVAIVTPMKENEEVNYDKLEELVEWQIEQGTDAIVIV
                     GTTGESSTLTTEEHSDVIRAAVRFAKHRVPVIAGTGSNCTREAIHLSEEAEKAGADGL
                     LAVTPYYNKATQGGLIRYYTEIAEAVKLPIIMYNVPGRTGCNIMPETAATLFKNVENI
                     VGIKEATGSLAQASKLMYLTDGKIDLYSGEDGIVVPLMAIGAIGVISVWSNVAPADVH
                     DMCAAFSAGDLLRARELQLKAQPLVDALFSEVNPIPVKKALNLMGLEVGPLRSPLTEM
                     TPENAAKLAEAMKNYGIKLA"
BASE COUNT          756 a         1099 c          759 g          971 t
ORIGIN      
        1 cgagaggatg cggttgacca cctcttcctc ctccagcagg cggtagtacg ggttcttcgg
       61 ctcctggtgc gtcgccttgt ccgcgataaa atagcgctca aacgtcgtca tttttgcttt
      121 tccgaacacc ggggaattct ggttttccca gataaaccac aagatatcca gtcccttttt
      181 cttctccccg ggatcaatcg cgtagtaccc ttttctcgcg taattctcca gcacatcgta
      241 gagcgccttg ccggaatatt cttttccgta cacatttact ctggtaaagg ttccgtcctc
      301 gttcatcggc acacagccgt ggtagagcag gttcccgttg tagaccttgt acaggctgcc
      361 ctgtgtgaac agaaaacgga cgtgacgctg taatttttca cagttcaaga acgcctgcgt
      421 gatccgctcc atgacgtcct cctcctccga tgacagttca tacggatgct tccagtcgat
      481 cgtcgggaag ttcagatcct tcatcgggta ttccttcccc tccacacaca ccgttccttt
      541 ttcgatattg atcttgtcca gcaaaagccg ctcatccatc ccgaactccg ggtgacgcat
      601 gatcatctgt ccctccagct tgaactggat caccgtaatc gccttgtgca ttttctcatc
      661 cagcatggcg tcacgcacat cgtactcatc ctcccggtaa tccagcttaa agcagatgca
      721 gggatccttg tcgtatctgc tcatggcaag tctggcgagc ggcaccagat tgatgccgta
      781 tccgtcctct aaaaggttca gattgccgta cttggcagag atacggatcg cgttgcagat
      841 gcagggtctg cttcccgccg ccgctcccat ccagtatata tcgtggttcc cccactgaat
      901 atccacggaa tgatgctgca tcaaggtatc cataatcaga ttcgggtacg gtcccctgtc
      961 gaaaatatct cccaccacat gtagatgatc caccacaagc ctgcggatca gattgcagaa
     1021 cgcaatcaca agctccggcg ccctgcctgt gcggatcaca gaccggatga tctcattata
     1081 ataagcttcc tgatcggaga catccggtct tccggtcagc agttcctcga tcacatacgc
     1141 aaaatctttc ggcagcgctt ttcgcacctt ggatctggta tattttgacg aagcgctctt
     1201 gcagatgcgg atcagccggt acagggagac cttataccag tcctccatat tttcttctgt
     1261 ctttaacacc tgctctagct tctgctccgg ataatagatc agggtcgcaa tcgctttctt
     1321 ctctcccgca ctgatcgcat tcccgaattc ttcctcgatc ttgcgcttaa tcgctccgga
     1381 accgttccgg atcacgtgct ggaactgatc gtactcgccg tgaatatctg taatgaaatg
     1441 ttctgttccc tttggcaggc ttaaaatcgc ctgcaggtta atgatctccg tcgctgccgc
     1501 agccacggtc ggatactgat ttgccagact ttttaaatac cgcagatctg tttccctata
     1561 tttttccata tgttccccct cagaatatca aaatcgaaga tacctctcaa atagtaacat
     1621 atccgcgcac tgctgaccag attttgttcg ttttcttccc tgtataaaaa tggaaacaga
     1681 aaacgtttcc acgctcctgt tcccattcga tccatctgta tccgctttca atctgcctta
     1741 tccgtctgcc tgtccaatca gtctgcctgt tccatcgttg cccggatcac atcctgcata
     1801 tcataaaatc ctgccttttt cccggcgagg aactttgccg cctccacagc cccttttcca
     1861 aagacgctgc gggaatatgc ggtatgcttg aactcaatga cctcgtcgag tcccgcgaag
     1921 atgacctcat gctccccgac gatgctgcct ccgcgcactg ccgagattcc gatctcgtgc
     1981 ggatctctct ttttgcgctc gctgcttctg tcatatttgt actcatagac atgtcccagc
     2041 gcatcattca tggagtccgc cagcgccagc gctgtgccgc tcggtgcatc cagcttctgg
     2101 ttgtggtgct tttccacgat ctccatgtca aatcctgccg gcgcaagaat cgttgccgcc
     2161 tgcgctaaaa gctgcatcag cagattgatt cccagcgaca tattcgcgga ctttaatact
     2221 gccacctgct ccgatgccgc ctttacctgc tccatctgtt cctccgacaa tcccgtcgtg
     2281 cacagaacca ccggaatctt tttatccaca ctatagacca gaagatcgtc cacggccttc
     2341 gcattggaaa aatcgatgat gacatccgcc ttcacatcgc agacggaaat attcgggaat
     2401 accggatagt catttttgat cccgtcatag agatccactc ccgccacgat ctcaatctgc
     2461 ggatcctcct tgcagattgc ggagatgacc tgtcccattt taccattgca tccattcatg
     2521 attgccttaa tcatcgtacg ttatcctctc ttctctgtac tgcccgctcc gcgtgccgca
     2581 tgcagccggc ggtgcagaca catactccgg cggatggcct tacggctcac ttccgccgga
     2641 gtcatctgtt cttttatgct aactttattc cgtaattttt catcgcttct gcaagcttcg
     2701 cggcattctc cggtgtcatc tccgtgagcg gggaacgaag cggtcccact tccagtccca
     2761 tcagattcaa tgctttcttc accggaattg ggtttacctc ggaaaacagc gcatccacaa
     2821 gcggctgtgc ctttaactgc agttctctcg cgcgcaaaag atcccccgcg gagaaagccg
     2881 cgcacatatc gtgcacatcc gccggcgcca cattcgacca cacggaaatc acaccaattg
     2941 caccgattgc catcagaggc accacgatgc cgtcctcgcc ggaatacaaa tcaatcttgc
     3001 cgtccgttaa gtacatcagc ttggatgcct gagccagact gccggtcgcc tccttgatac
     3061 ccacaatgtt ctccacgttc ttaaagagtg tggctgcggt ctccggcata atattgcagc
     3121 cggtacgtcc aggaacgttg tacatgataa tcggaagctt taccgcctcg gcaatctctg
     3181 tatagtaacg gattaatccg ccctgtgtcg ctttgttgta gtacggggtc acagccaaaa
     3241 gtccgtctgc tcctgccttt tccgcctctt cggaaaggtg aatcgcctct ctggtgcagt
     3301 tggaaccggt gccggcaatg accggaacac gatgctttgc aaaacgcact gctgcccgga
     3361 tcacatcgga atgctcctcc gtagtgagcg tcgaactctc tccggtcgtt cccacaatca
     3421 caatcgcatc cgttccctgc tcaatctgcc attcgaccag ttcctccaac ttgtcgtaat
     3481 ttacctcttc attttccttc atgggtgtaa caatcgctac accagcgccc ttgaatatcg
     3541 ccatgccaat cctccgtttc tcctatgtta atatatgccc atggt
//
LOCUS       DBMDQJ010000194         3570 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9583_length_3570_cov_1.527241, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000194 DBMDQJ010000000
VERSION     DBMDQJ010000194.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9583_length_3570_cov_1.527241"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            64..963
                     /locus_tag="ACZO9R_05185"
     CDS             64..963
                     /locus_tag="ACZO9R_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HIR0912491.1"
                     /translation="MKKLKQFLFIFVPLLLVIAIQFLATYFAMGLSLLIENGWYSVTG
                     SAGFLDIVDDAFSLWSSQRFNTGVLLIYNAMSIAVFGLWYYCRYGGNYRPVLRQTFHP
                     AAVAGIVMLMPGTQYLTTYIMSFVASLFPHWMDAYESLLETAGLDDQISILMVICSVI
                     FAPFCEELVFRGVTMHQAKKCLPFWAANLLQALLFGIFHMNMIQGIYAFCLGLVLGYV
                     CNRGGSIYYSLLLHMLFNFWGTVLSGLIPFGDTTFAFIFWFLFGIAMTVGGLVAFNFG
                     IQRLQTAGRASAMPLSDIPSGTE"
     gene            complement(926..1987)
                     /gene="hisC"
                     /locus_tag="ACZO9R_05190"
     CDS             complement(926..1987)
                     /gene="hisC"
                     /locus_tag="ACZO9R_05190"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR0912492.1"
                     /translation="MENWESYVRKVVPYVPGEQPQAERMIKLNTNENPYPPAPGVQRA
                     LAGFNPDTLRLYPEPTCKVLVDAIASYYGLGSDQVFVGVGSDDVLAMIFLTFFNSGKP
                     VFFPDITYSFYDVWADMLRIPYETKALDDAFEIRVEDYYGENGGVIFPNPNAPTGILM
                     PLDKIEDIIAHNRDVIVVVDEAYIDFGGVSALPLIEKYDNLLIVQTFSKSRSMAGMRI
                     GYAMGNAKLIRYINDVKYSFNSYTMNRTALALGVEAIRDRAYFEETRKKIIETREWTK
                     KELRALGFTFGDSMSNFIFASHERVPATELFEALRREHIYVRHFTKERINNYLRISIG
                     TREEMETFIRFLKEYLKEA"
     gene            complement(1999..3210)
                     /locus_tag="ACZO9R_05195"
     CDS             complement(1999..3210)
                     /locus_tag="ACZO9R_05195"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0912493.1"
                     /translation="MVSKRIQKALEGNSAIRAMFNEGREMAAKYGEENVFDFSLGNPA
                     TPAPKALNDAIRDLLDEADAKGAAGSLGIHGYMANAGYEEVRQAIAENLNERFGTKFT
                     AHNLVMTVGAAGGLNIIFKTILDPDDEVIVFAPFFGEYRQYAANFDAKIVIVQPNLET
                     FQPDLADLEAKITARTKALIVNTPNNPTGVIYKPQTMEQIGAILEKKQAEFGTDIYLI
                     SDEPYRELVYDGNKEDFLTKYYRNTLVGYSFSKSLSLPGERIGYVVVPDEAADAEELI
                     RGIEISNRTLGFVNAPSLIQKAVARCLEEKTDVTFYDENRSRLYEGLTKLGFTCIKPE
                     GAFYLWVKSPVENEEEFVNEGKKFNLLMVKGSAFGCPGFVRLAYCVSHETVEHSLPAF
                     AKLAAVYGLEK"
BASE COUNT          789 a         1056 c          765 g          960 t
ORIGIN      
        1 atagaagtat ctggaaattg caagaacttt atttcatacc aacgataact ggagattata
       61 ataatgaaga aattaaaaca attcctgttt atttttgtcc cgctgcttct tgtgattgcg
      121 attcagtttc tcgcaaccta ctttgccatg ggactttccc tgctgatcga gaacggctgg
      181 tactctgtca ccggcagcgc ggggttcctc gatattgtag atgacgcctt ttccctgtgg
      241 agttcccaac gcttcaacac cggcgtactg ttaatctaca atgccatgtc gatcgccgtg
      301 ttcggtctgt ggtactactg ccggtatggc ggcaattacc gccccgttct gcgtcagacg
      361 tttcatcctg ctgcagtcgc gggcatcgtc atgctgatgc ctggcacaca gtatctgaca
      421 acctatatca tgagctttgt cgcgtcgctc tttccgcact ggatggatgc atacgaatcc
      481 ctgctcgaga cggcaggcct cgatgatcag atctccattc tgatggtcat ctgctctgtc
      541 atcttcgccc cgttctgcga ggagctggtc ttccgcggtg tcaccatgca tcaggcaaag
      601 aaatgtcttc ctttctgggc agccaatctg ctgcaggcgc tgctttttgg catcttccac
      661 atgaacatga tccagggcat ctacgcattc tgtctgggac ttgttttagg ctatgtctgc
      721 aaccgcggcg gcagcatcta ttactcgctc cttttgcaca tgctgtttaa cttctgggga
      781 accgtgctct ccgggctcat cccgtttggt gacacgacgt ttgccttcat cttctggttc
      841 ctgttcggca tcgccatgac ggttggcggt ctggtcgcct ttaacttcgg catccaaaga
      901 cttcagacgg ccggccgcgc atccgctatg cctctttcag atattccttc aggaaccgaa
      961 taaacgtctc catctcttct ctcgttccga tgctgatacg caggtagttg ttgatgcgtt
     1021 ccttcgtaaa atggcgcaca tagatatgct ccctgcgcag cgcctcgaac agttctgttg
     1081 ccggcacgcg ctcgtgcgaa gcaaagataa aattgctcat cgaatccccg aacgtaaatc
     1141 ccaacgcccg caattctttt ttcgtccact ctctcgtctc gatgatcttt tttctggtct
     1201 cctcaaaata agcacggtca cggatcgcct ccacaccgag tgcaagcgct gtccggttca
     1261 tcgtataaga attaaacgaa tacttcacgt cattgatata acggatcagc ttcgcatttc
     1321 ccatcgcata tccgatgcgc attcccgcca tggaacgcga tttggaaaat gtctgcacga
     1381 tcagaagatt gtcatatttt tcaatcagcg gcagcgccga cacccctcca aaatcaatat
     1441 acgcctcgtc caccacaacg atcacgtcgc ggttgtgcgc gatgatgtcc tcgatcttat
     1501 caagcggcat cagaatgccg gtcggtgcat tcgggttcgg gaatatcact ccgccgtttt
     1561 ccccgtaata atcctccacc cggatctcaa acgcatcatc aagcgctttc gtctcatacg
     1621 gaatgcgcag catatctgcc cacacatcat aaaaagaata cgtaatgtcc gggaagaaaa
     1681 ccggctttcc ggaattaaag aatgtcagaa aaatcatcgc cagcacatca tccgaaccga
     1741 ccccgacaaa tacctgatcg ctgcccagtc cataatatga cgcaatcgca tccacaagca
     1801 ccttacaggt cggttccgga tacaggcgca gcgtatccgg gttaaatccg gcaagcgccc
     1861 tctgcacccc cggcgcgggc ggatacggat tctcgttggt gttcagcttg atcatccgct
     1921 ccgcctgcgg ctgctccccc ggcacatacg gcaccacttt tctcacatat gactcccagt
     1981 tctccatcct ttttcctcct acttttccag tccgtacact gccgccagct tcgcaaatgc
     2041 aggcagggaa tgttccacgg tctcatggga cacacaatat gcgagacgca caaatcccgg
     2101 acagccaaat gcgcttccct ttaccatcaa aagattaaat ttctttccct cgtttacaaa
     2161 ttcttcttcg ttttccaccg gtgacttcac ccacaggtaa aacgccccct ccggcttgat
     2221 gcaggtaaat ccgagctttg tcagtccttc gtagagtctg ctgcggttct catcgtaaaa
     2281 cgtgacatcc gtcttctcct caaggcagcg cgccacagct ttctgaatca gggacggtgc
     2341 gttgacaaat ccgagtgtcc ggtttgagat ctcaatgccg cggatcagct cttcggcgtc
     2401 cgccgcttca tccggcacca cgacataacc gatacgctct cccggaaggg aaagggattt
     2461 ggagaaagaa tatcccacca gggtattgcg ataatatttc gtcaggaaat cctctttgtt
     2521 cccgtcatac accagctccc ggtacggctc gtccgagatc agataaatgt ctgttccaaa
     2581 ttcagcctgc tttttctcta aaatagctcc gatctgctcc atcgtctgtg gcttgtagat
     2641 cacgccggtc gggttgttcg gcgtattcac gataagcgcc ttcgtccgcg cggtaatctt
     2701 tgcctccaga tcggcaagat ccggctgaaa cgtctccaga ttcggctgta caatcacaat
     2761 ctttgcgtca aaattcgccg catactggcg atattctccg aagaatggtg caaacacgat
     2821 gacctcatcg tccggatcga gaatcgtctt aaaaataatg ttgagcccgc ctgcggcgcc
     2881 gactgtcatc accagattgt gcgccgtaaa ctttgttccg aaacgttcgt tcaaattctc
     2941 cgcgatcgcc tggcgtactt cctcgtagcc cgcatttgcc atataaccgt gaatgcccag
     3001 agaacctgcc gctccctttg catccgcctc atcaagaaga tcgcggatcg catcgtttaa
     3061 cgccttcggc gccggggttg ccggattgcc gagtgagaag tcaaacacat tctcctcccc
     3121 gtactttgca gccatctccc tgccctcgtt gaacatggcg cggattgccg aattgccctc
     3181 cagcgctttt tgtattctct ttgataccat aatacctatc ctcttttctg cggtctgacc
     3241 cgcatcgttt tctcattcct ttactttatc aaattaccac gcgaaagtaa acggtcattt
     3301 tgcaaaaatt tgcttcatgc acaagtataa cacatttatg ggaaattgaa aaaagaacgg
     3361 acaacttccc gagaaaatat ccccgccccg ttatccgttc ttttcttctc tcttcctatg
     3421 cgttcactgt atccacaaag cgcatgaagg ctgctcttcc ctcttctgta cacttgtaga
     3481 ctcctgcgtc ggtgagaacc tccttaaata ccagaccgat ctctgtctga acgatctcat
     3541 gtaacctctc cggtgtgatc tcgccctcca
//
LOCUS       DBMDQJ010000195         3549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9649_length_3549_cov_1.449246, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000195 DBMDQJ010000000
VERSION     DBMDQJ010000195.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9649_length_3549_cov_1.449246"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="ACZO9R_05200"
     CDS             <1..638
                     /locus_tag="ACZO9R_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR0912494.1"
                     /translation="SVNVAIISRMLGNWLGFSEDAQNTLTLCGLLHDIGKSRIPASII
                     GKPGRLTSEEFEQIKRHPLLGYELLKNQPLDPHIKNAALMHHERCDGSGYPFGLYASD
                     IDDYASIIAVADVYDAMTADRCYRLGLCPFEVIAQFEQEGLQRYKPRYILTFLEHIAH
                     TYLHNRVLLNNGQTGEIIFINKRLTRPTIQIAPSRFINLEEHPELYIQAII"
     gene            665..976
                     /locus_tag="ACZO9R_05205"
     CDS             665..976
                     /locus_tag="ACZO9R_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR0912495.1"
                     /translation="MADNHNTNEDEDILVTLDLDDGSQVECEILTIFTVNEQDYIALL
                     PLTEAGEPNEDGEVYIYRYAEDSDGNPSLDNILDDAEYEAVSDRFDEWLDEAEFNDME
                     D"
     gene            complement(957..2810)
                     /locus_tag="ACZO9R_05210"
     CDS             complement(957..2810)
                     /locus_tag="ACZO9R_05210"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006779775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR0912496.1"
                     /translation="MSFNPSQREAIRHKDGPMLVLAGPGSGKTLVITERTRHLITEYG
                     INPANILVITFTRAAATEMKERFLRLMGGKSSAVTFGTFHAVFFMILKHAYHYQAENI
                     IREEQRVSCMREIIKRHRLEYEDESEFIAALLGEIGLVKNSGISLDHYYSTNCGEEVF
                     RRVYSEYHEYLYAHHLIDFEDMLLYTWELFKERKDILAAWQKKYPYILIDEFQDINQI
                     QFEVVRLLAGEQRNLFVVGDDDQSIYRFRGAKPEIMLRFEREIPGTKTVLLDTNYRSQ
                     ANIVEDALNLIGHNKERFQKRIRAALPAETGVEYAVFEDQRKENLRIIRDICDAVERG
                     KSYRDFAVLFRTNTQPRLLMEQMMEYNIPFRTRDRIPNLYEHWIAKDIFAYIRIAQGS
                     RERRDFLQIMNRPKRYISRDSLDEDTVAFDVWEWYFREAEQPWVAERIVTLEHDVMQL
                     RRMNPFAAINYIRKGIGYDEFCAEYADYRRIRADELYDVLDELQESARGYEDYDGWFA
                     HIENYTRELERLYQSQEKQSESVALSTLHSAKGLEYENVYLIDVNEGVMPYKKAVLDQ
                     DIEEERRMFYVGMTRAKKYLHLFSVKQMNQKDADISRFIAEAGERLSPPCR"
     gene            complement(2824..>3549)
                     /locus_tag="ACZO9R_05215"
     CDS             complement(2824..>3549)
                     /locus_tag="ACZO9R_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009461066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HIR0912497.1"
                     /translation="PLITDENHKKLSKRSGHSSFEDLLEQGFLSEAVVNYIALLGWSP
                     EDNREIFTLDELIKEFDYHRISKSPAVFDYTKLKWMNGEYIKAMDFDRFYEMAEPYLR
                     EVIHKDMDLKKIAAMVKTRIEVFPEIRDHIDFFEELPEYDIAMYTHKKMKTNAETSLE
                     VLSDVLPLLEAQEDFSNDALYATLLKYVEEKGVKNGYVMWPIRTAVSGKQMTPGGATE
                     LMEVLGKEESIARIKKGISMLQK"
BASE COUNT          826 a         1017 c          810 g          896 t
ORIGIN      
        1 acagcgtcaa cgtcgccatt atctcccgca tgctcgggaa ctggctcggt ttttcggagg
       61 atgcccagaa cacactgact ctctgcggtc ttttacacga tatcgggaaa tcccggattc
      121 ctgccagcat catcggcaag ccgggcaggc tgacttccga agagtttgaa cagatcaagc
      181 gccatcccct tttagggtac gagctgctta aaaaccagcc tctcgatccg cacataaaga
      241 acgctgccct gatgcatcac gagcggtgtg acggaagcgg ttatccgttc ggtctgtacg
      301 catcggacat tgacgactac gcttcgatca tagccgtcgc ggatgtatat gatgccatga
      361 ccgccgaccg ctgttatcgt ctggggctgt gtccgtttga agtgatcgcc cagtttgaac
      421 aggaaggctt gcagcgctac aagccgcgct atattctcac ctttctcgaa cacattgcgc
      481 acacttacct gcacaaccgg gttctgttga acaacggaca gaccggagag attattttta
      541 tcaataagcg tctgacgaga cctacgatcc agatcgcccc ctcacgtttc atcaatcttg
      601 aggagcatcc ggagctctac attcaggcga ttatttaata acatccaata aaggagatat
      661 catgatggca gacaaccata acaccaacga agacgaggat atcctggtca ccctggatct
      721 ggatgacggc agccaggtag aatgtgagat tttaaccatt tttaccgtca acgagcagga
      781 ctatatcgcc cttcttccgc tcaccgaagc cggcgagccg aacgaggacg gcgaggtcta
      841 tatctaccgc tatgcggagg atagcgatgg caatccgtct ctggataata ttttagacga
      901 cgccgaatac gaagccgtat ccgaccgctt tgatgagtgg cttgacgagg cagaatttaa
      961 cgacatggag gactgagacg ctctcctgcc tcggcaataa agcgcgagat atctgcatct
     1021 ttctgattca tctgtttgac ggaaaagaga tgcagatatt tttttgcccg cgtcataccg
     1081 acgtaaaaca tccggcgctc ctcctcgatg tcctgatcca gcaccgcctt cttgtagggc
     1141 atcactccct cgttcacgtc gatcagatag acgttttcgt actcaagccc cttggcggaa
     1201 tgcagcgtgg aaagcgctac gctctcactc tgcttttcct ggctctggta caggcgttcc
     1261 aattccctgg tgtaattctc gatgtgcgca aaccatccgt cataatcttc atagcctctt
     1321 gcactttcct gtagttcatc cagcacatca taaagttcat ccgcccggat acgcctgtaa
     1381 tctgcatact cggcacagaa ctcgtcatat ccgatgcctt tccggatata gttgatggcg
     1441 gcaaacggat tcatccggcg aagctgcatc acatcatgct ccagcgtgac gatccgctcc
     1501 gccacccacg gctgttccgc ctctctgaaa taccattccc acacatcgaa tgccaccgtg
     1561 tcctcgtcca atgaatctct gctgatatag cgcttcgggc ggttcatgat ctgcagaaaa
     1621 tcacggcgtt cccggcttcc ctgcgcgatc cggatatagg caaaaatatc ctttgcgatc
     1681 cagtgctcgt aaaggttcgg tatgcggtct ctcgtcctaa acggaatatt gtactccatc
     1741 atctgctcca tcagaagacg cggctgtgtg ttggtgcgga acagcacagc aaaatcacgg
     1801 tagctctttc ccctctccac cgcatcacaa atatcgcgga tgatccgcag attctccttc
     1861 cgctgatcct caaaaacggc atactccacc ccggtctccg ccgggagtgc cgcgcggatg
     1921 cgcttctgga agcgctcctt attgtgcccg atcagattca gcgcatcctc gacaatattg
     1981 gcctgcgacc ggtaattcgt gtctagaagc acggttttcg tccccggaat ttcacgctca
     2041 aaccgcagca taatctccgg ctttgctccg cggaagcggt agatcgactg gtcgtcatcc
     2101 cccacaacaa acagatttct ctgctcaccc gcaagcagcc tgaccacctc aaactggatc
     2161 tggttgatgt cctggaactc gtcaatcaga atgtagggat attttttctg ccaggcagcc
     2221 agaatatcct tccgctcctt gaaaagctcc catgtataca aaagcatatc ctcgaagtca
     2281 atcagatgat gcgcatagag atattcgtgg tactcgctgt aaactctccg gaacacctct
     2341 tccccgcagt tcgtggaata gtaatgatcg agtgaaattc cgctgttttt cactagaccg
     2401 atctccccca gaagcgctgc gatgaactcc gactcatcct catattccag cctgtggcgc
     2461 ttaatgatct cacgcatgca gctcacccgc tgctcctcgc ggatgatgtt ctccgcctga
     2521 tagtggtagg cgtgctttaa gatcataaaa aagacggcgt ggaaagttcc aaacgtcact
     2581 gcggaagatt ttccgcccat caggcgcaaa aaacgttcct tcatctccgt tgccgccgct
     2641 cttgtaaacg tgatgacaag aatgtttgcc ggattgattc catattctgt tatcaaatgt
     2701 ctggtgcgtt ccgtaatgac aagggtcttg cccgaaccgg gcccggccag tacgagcatg
     2761 ggaccatcct tgtgacggat ggcctcacgc tgagacgggt taaaactcat agattacctc
     2821 tgattatttc tgtaacatgg aaatgccttt tttgattctc gcaatggatt cctctttgcc
     2881 taaaacctcc atcagctctg tcgctccgcc cggcgtcatc tgtttgccgg acactgcggt
     2941 acggatcggc cacatcacat agccgttctt cacgcccttt tcctcaacat acttaaggag
     3001 cgtcgcatag agtgcatcat tggaaaaatc ctcctgcgct tccagcagcg gcaggacgtc
     3061 gcttaaaacc tccagcgagg tctctgcatt ggtcttcatc ttcttgtgtg tgtacatcgc
     3121 aatatcgtac tccggcagtt cctcgaagaa gtcgatgtgg tcgcggatct ccgggaaaac
     3181 ctcgatacgg gtcttcacca tcgctgcaat cttcttcaaa tccatatcct tgtgaatgac
     3241 ctcgcgcaga tacggctccg ccatctcata aaaacggtca aaatccatcg ccttgatgta
     3301 ctccccgttc atccacttta acttggtgta atcgaacacc gccggggatt tgctgattct
     3361 gtgatagtcg aactctttga tcagctcatc cagggtaaag atctcacggt tgtcctccgg
     3421 gctccatccc aaaagtgcga tgtagttgac cactgcctcg gacaggaatc cctgctctaa
     3481 gagatcctca aaggaagaat gtccgctgcg cttggaaagc tttttatggt tctcatccgt
     3541 aattaacgg
//
LOCUS       DBMDQJ010000196         3541 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9671_length_3541_cov_1.388663, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000196 DBMDQJ010000000
VERSION     DBMDQJ010000196.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9671_length_3541_cov_1.388663"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="ACZO9R_05220"
     CDS             complement(<1..1647)
                     /locus_tag="ACZO9R_05220"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR0912498.1"
                     /translation="MFREDFVWGVASSAYQVEGTDAADGRGACVWDMFAADGRVFEHQ
                     NADTSCDHMHHYKEDIALMKHLGIRAYRFSLNWARILPEGTGRVNEKAIAMYRDMILT
                     MKENGITPYLTLFHWEFPQALYERGGWQNPDVVQWFGEYAKVVAENFSDICEYFITIN
                     EPQCVVGLGHLSGVHAPGVKLPVAQTFLVAHHLLMAHGQAVINLRRYAVRPVKIGFAP
                     TGGVAYPYTDRPEDIEAAKKVYFGFYNPIDNWTWNVSWFSDPVFLGHYPEEGLKKFAP
                     YLPEITQEDMELIYQPLDFMGQNIYNRYYVRAGADGEPEFVDRAPGFPKTGSDWPVTP
                     EAFYYGIKFLTERYPLPLYITENGMSCHDMVSSDGRVHDPNRITFLDSYIGAMQKAYD
                     EGANVAGYFLWTFLDNFEWADGYKQRFGIVYVDFASQRRIVKDSAFWYQKIIETNGRM
                     LTMNQTTKEILFLDPVCTHNIWGGTRLRTEFGYPVEGDDIGECWGISAHPNGDGSIRN
                     GVYAGMKLSKVWEEHPEVFGNLAYDRFPLLTKIIDAKADLS"
     gene            complement(1761..2924)
                     /locus_tag="ACZO9R_05225"
     CDS             complement(1761..2924)
                     /locus_tag="ACZO9R_05225"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR0912499.1"
                     /translation="MNMQIDLEKGIANVGDVSRMKEVMARAKRGGRLVIGFIGGSITQ
                     GSLSSTPQTCYAYRVYTWWKETFPQAEFVYCNAGIGGTTSEFGAARVGTDLLAERPDF
                     VIVEFSVNDESTEHFKETYEGLVRKILGAAWNPAVLLVHNVFYHNGANAQMMHAAIGR
                     HYDLPSVSMQSSIYPEVVAGRIENRAITPDDLHPNDEGHALVASVITYFLEQVRSGKL
                     TGSNTSFAPGAKLPAPLTKNAYEDSVRIRNAAAPDMAGLQQPTLDGFTPDETAQNEIT
                     DCFRYGWTAKKTGDAITFLAEGSCIAVQYRKSVKLPAPAALAVVDDDTEHAVRLDANF
                     DETWGDKLELATLLEHGKEGRHTVTIRLAETHEDDAVPFYLVSVIAAGRRGRD"
     gene            complement(2965..>3541)
                     /locus_tag="ACZO9R_05230"
     CDS             complement(2965..>3541)
                     /locus_tag="ACZO9R_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR0912500.1"
                     /translation="SNSWYSYAWMTARKLGAELHDTSQCGIALLDGTGWFGAPAYYGV
                     ESCYDKLEYNPDLGEVKKWDFSRYTPQVVVVAIGQNDNHPVDYMAADPEGSAAEHWRK
                     RYREFIEILMKHYPKAQIILATTILKHHPNWDAAIETVCGQIASERVHHFLYRRNGSG
                     TPGHIRIPEAEEMSEELAFYIRSLGDEIWDV"
BASE COUNT          765 a         1134 c          789 g          853 t
ORIGIN      
        1 actcagatcc gccttcgcat cgatgatctt tgtcagaagc gggaagcggt cgtaggcgag
       61 gttcccaaat acctccgggt gctcctccca cacttttgac agcttcatgc cggcgtacac
      121 accgttcctg atgctgccgt cgccgttcgg atgtgcggag atgccccagc actcgccgat
      181 gtcgtcaccc tccaccggat agccgaactc cgttctaagc cgggtaccgc cccagatatt
      241 gtgcgtgcac accggatcta aaaataagat ttcttttgtt gtctgattca ttgtcaacat
      301 ccttccattc gtctcgatta ttttctgata ccagaaagcg gaatccttca caatccgcct
      361 ctggcttgca aaatccacat aaacaatgcc aaaccgctgc ttgtatccgt ccgcccactc
      421 gaaattatcc aggaaggtcc agaggaaata gccagctaca ttggcgccct catcatatgc
      481 cttctgcatc gccccgatat aggaatccag gaaggtaatg cggttcgggt catgtaccct
      541 gccgtcactg ctcaccatat catggcagga cataccgttc tccgtaatgt agagcggcag
      601 cggatagcgc tccgtcaaaa acttaatgcc gtagtaaaac gcctccggcg tcaccggcca
      661 gtccgatccc gtcttcggga accccggcgc acggtctaca aattccggct cgccgtctgc
      721 gccggcgcgg acgtagtacc tattatatat attctgtccc ataaaatcaa gcggctgata
      781 gatcagctcc atatcctcct gtgtgatctc cggaagatac ggcgcaaatt ttttcagccc
      841 ctcctcgggg taatgtccaa ggaataccgg atcggaaaac cacgatacat tccaggtcca
      901 gttatcgatc ggattgtaaa atccgaaata cacctttttc gccgcctcaa tgtcctccgg
      961 ccggtcggtg tacggatatg ccacgccgcc ggtcggagcg aaaccgatct tgaccggtcg
     1021 gaccgcatat ctgcgcaggt tgatgaccgc ctgcccgtgc gccatcagaa gatgatgcgc
     1081 caccagaaac gtctgcgcca cgggaagttt cacgcccgga gcatggacac cgctcagatg
     1141 tccgaggccg accacgcact gcggctcgtt gatcgtaata aaatattcgc agatatcact
     1201 gaaattctcg gcaaccacct tcgcatattc tccgaaccac tgtactacgt ccggattctg
     1261 ccagccgccc cgctcataaa gcgcctgcgg gaattcccag tgaaacagtg tcagatacgg
     1321 tgtgatgccg ttctccttca tcgtgagaat catatcacgg tacatggcga tcgctttctc
     1381 attcacgcgc ccggttccct ccggcagaat ccgcgcccag ttcagtgaaa aacggtacgc
     1441 ccggatcccc agatgcttca tcagcgcaat atcttcttta taatgatgca tatgatcgca
     1501 gcttgtgtct gcattctgat gttcaaacac cctgccgtcc gccgcaaaca tatcccagac
     1561 gcacgcgcct ctgccgtccg ccgcgtccgt tccctctacc tgataggcgc tgctcgcaac
     1621 accccacaca aaatcctctc gaaacattct tataacctcc tgctcctgca atcgcagtgt
     1681 ctctctctat cttccaacat tttttctgat tttgcaatcc ttttacacgc acattctgca
     1741 aaataggctg tctcttgccg tcagtctctt cctcttctcc ccgccgcaat cacggacacc
     1801 agataaaacg gaaccgcatc gtcctcatgc gtctctgcga gtcggatcgt gacggtatgc
     1861 cgcccctctt ttccgtgctc taaaagcgtc gcaagttcca gtttgtctcc ccaggtctca
     1921 tcaaaatttg catccagacg caccgcatgc tccgtatcgt catccacgac cgccagtgca
     1981 gccggcgcag gaagctttac cgacttccgg tactgcacgg caatgcagct gccctccgcc
     2041 aaaaaggtga tggcatcccc cgtctttttc gccgtccagc catagcggaa gcagtccgtg
     2101 atttcattct gcgcggtctc atcgggcgta aatccatcca gcgtgggctg ctgcaatcct
     2161 gccatatccg gtgccgcggc attgcgaatg cggacggaat cctcgtaggc attttttgta
     2221 agcggagccg gaagctttgc ccccggtgca aaactggtat tgctgccggt cagttttccg
     2281 cttcgtacct gctccagaaa ataagtaatc accgacgcca ccagcgcatg cccctcatcg
     2341 ttcgggtgca gatcatccgg cgtgatcgcg cggttttcaa tccgtcctgc caccacctcc
     2401 gggtaaatag agctctgcat gctgaccgac ggcagatcat aatgtctgcc gattgccgcg
     2461 tgcatcatct gcgcgttggc tccattgtgg taaaacacgt tgtgtaccag aagaaccgcc
     2521 ggattccacg cggcaccaag gattttccgc accagtcctt cataggtctc cttaaaatgc
     2581 tccgtgctct catcattgac ggaaaattcc acaatcacaa agtccggtct ctccgccaga
     2641 agatccgtcc ccacacgcgc cgcgccgaac tccgatgtcg tgccgccgat tccagcattg
     2701 cagtagacaa actccgcctg cggaaaggtc tctttccacc atgtatacac acgataggcg
     2761 tagcacgtct gcggtgtcga ggaaaggctt ccctgcgtga tcgaaccgcc gataaaaccg
     2821 attaccagac gccccccgcg ttttgcgcgc gccatgacct ccttcattct gctcacatct
     2881 cccacatttg caattccctt ttccagatca atctgcatat tcatatttct gctccttctc
     2941 tctttctttt ccctttattg tctctcaaac atcccagatt tcatctccga gcgaacggat
     3001 atagaaagcc agctcctccg acatctcctc cgcctccgga atccggatgt gtccgggcgt
     3061 tccggagccg tttctccggt acaaaaagtg gtgcacgcgc tcacttgcaa tctgcccaca
     3121 caccgtctca atcgccgcat cccagttcgg gtgatgtttt aagatggttg tcgcaagaat
     3181 gatctgcgcc tttggatagt gcttcatcag tatctcaatg aattcccggt agcgcttccg
     3241 ccagtgctct gccgcactgc cctctggatc cgctgccata tagtccaccg gatgattgtc
     3301 attctgcccg atggcaacca caaccacctg cggcgtatag cgtgaaaaat cccatttctt
     3361 tacctctcca agatccggat tatactctag cttgtcgtaa caggactcca caccgtagta
     3421 tgccggcgca ccgaaccatc ccgtcccgtc aagcagcgca atgccgcact gtgaggtatc
     3481 gtgaagttct gccccaagct ttcgcgctgt catccaggca taggaatacc agctgttgga
     3541 a
//
LOCUS       DBMDQJ010000197         3528 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9724_length_3528_cov_1.567260, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000197 DBMDQJ010000000
VERSION     DBMDQJ010000197.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9724_length_3528_cov_1.567260"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1037)
                     /locus_tag="ACZO9R_05235"
     CDS             complement(<1..>1037)
                     /locus_tag="ACZO9R_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR0912501.1"
                     /translation="QKIFLSAQKDVEQEEPGADDVYHIGVIAEVKQVIKLQNNIVRIL
                     VEGIERAELSAFAQTDPYLLAEVAPCVLPEEGLSEEAKAAMVRSVQETYSRYQTVNPR
                     AGKELLRQIGTIQDLPKLMDQVANNLPVSYEEKQKILEAMTLTERYEVLMALLLKEIE
                     ITAIQNEFQSKVKERVDKNQKEYILREQMKLIREELGEDNTESDADEYQKALDALDAD
                     QEVKNRIKKEIDRFKSISANSSESAVTRGYIETLLELPWNKASEDNKDLANAERILEE
                     DHYGLEKVKERMLEFLAVRNLTSKGESPIICLVGPPGTGKTSIARSVARALDKKYVRI
                     CLGGVRDEAEI"
     assembly_gap    1038..1047
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1048..1171)
                     /locus_tag="ACZO9R_05240"
     CDS             complement(<1048..1171)
                     /locus_tag="ACZO9R_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014269.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR0912502.1"
                     /translation="MLDTEILKIPAVALRGMVILPGMIAHFDISRDRSIRAIEQC"
     gene            complement(1177..2436)
                     /gene="clpX"
                     /locus_tag="ACZO9R_05245"
     CDS             complement(1177..2436)
                     /gene="clpX"
                     /locus_tag="ACZO9R_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HIR0912503.1"
                     /translation="MAGRSDDEIRCSFCGKSQGQVRKLIAGPKGAYICDECVDICAEI
                     IEEEFENEEETGAAEETEQINLLKPEELKAFLDDYVIGQDQAKKVLSVAVYNHYKRIM
                     AGDDLGVELQKSNILMLGPTGSGKTLLAQTLARVLNVPFAIADATTLTEAGYVGEDVE
                     NILLKIIQAADYNIERAQHGIIYIDEIDKISKKSENVSITRDVSGEGVQQALLKILEG
                     TIASVPPQGGRKHPQQELIQIDTTNILFICGGAFDGLEKIIETRMDQKAIGFNADIKS
                     KNEYNIGDVLKHALPQDFVKFGLIPEFIGRVPVTVSLDMLDRDALIRILKEPKNALTR
                     QYEKLFELDGVKLAFDADALEAIADKALERKTGARGLRAIMEAVTLDLMYHIPSDESI
                     TECEITKDLVEEKLSIEEQKLKTIEEK"
     gene            complement(2485..3090)
                     /gene="clpP"
                     /locus_tag="ACZO9R_05250"
     CDS             complement(2485..3090)
                     /gene="clpP"
                     /locus_tag="ACZO9R_05250"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HIR0912504.1"
                     /translation="MSLVPYVVEQTSRGERNYDIYSRLLKDRIIFLGEEVNETTASLV
                     VAQLLFLESEDPNKDIQLYINSPGGMVTAGMAIYDTMQYIKCDVSTICIGLAASMGAF
                     LLAGGTKGKRYALPNAEVMIHQPSGGAKGQATEIQIAAENILKTKKKLNEILAANTGK
                     PYDVVAADTERDYYMSAEEAKAYGLIDDVIIRKDTKKEESK"
     gene            complement(3196..>3528)
                     /gene="tig"
                     /locus_tag="ACZO9R_05255"
     CDS             complement(3196..>3528)
                     /gene="tig"
                     /locus_tag="ACZO9R_05255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741851.1"
                     /note="Tig; RopA; peptidyl-prolyl cis/trans isomerase;
                     promotes folding of newly synthesized proteins; binds
                     ribosomal 50S subunit; forms a homodimer; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR0912505.1"
                     /translation="GLTLDKLKEQVRPEAETRIKSSLVLEQIAKDEKIEITDEELDAE
                     IEKMAAQYGMEADKLKEYIGDNEKESMKRDLAVTKAVDLIMENVKERAKAKSKKEKEA
                     EEKEGTEE"
BASE COUNT          715 a         1051 c          729 g         1023 t
ORIGIN      
        1 gatctccgcc tcatcgcgca caccgccgag gcagatccgc acgtatttct tatccagcgc
       61 acgcgccaca gagcgcgcaa tgcttgtctt gccggttccc ggaggtccca ccagacagat
      121 gatcgggctt tctcccttgc tcgtcagatt gcgcaccgcg agaaactcca acatacgctc
      181 cttgactttc tccagaccgt agtgatcttc ctccagaatc cgctctgcgt ttgcaaggtc
      241 tttgttgtcc tcggacgctt tattccacgg aagctccagc agcgtctcga tataaccgcg
      301 ggtcaccgcg ctctccgatg aattcgcgct gatgctcttg aagcggtcaa tctccttctt
      361 gatgcggttt ttcacttcct ggtcggcatc cagcgcatcc agtgctttct ggtactcgtc
      421 cgcatccgat tccgtattgt cctcgccgag ttcctcccgg atgagcttca tctgctcgcg
      481 cagtatgtat tccttctggt tcttatccac gcgctctttg actttgctct ggaattcatt
      541 ctggatggca gtgatttcaa tctcttttaa aaggagcgcc atcaatacct catagcgctc
      601 cgtaagtgtc attgcctcca gaatcttctg cttttcttcg taagaaaccg gcagattgtt
      661 cgcaacctga tccatcagct tcggcagatc ctgtatggta ccgatctggc gcagcaattc
      721 ttttccggca cgcggattta ccgtctgata ccggctgtat gtctcctgta cactgcgcac
      781 catcgcagct ttggcctctt ccgaaagccc ctcctccggc agcacgcacg gcgccacctc
      841 tgccagaaga tacggatccg tctgtgcaaa agccgaaagt tcagctcgct cgataccctc
      901 aaccagaatt cttacgatat tgttttgtaa tttgattacc tgctttactt ctgcgatcac
      961 accgatatgg tatacatcgt ccgctcccgg ttcttcctgt tcgacatcct tctgcgccga
     1021 caggaaaatc ttctgggnnn nnnnnnntgc actgttcgat cgcacggatg gaccggtcac
     1081 ggctgatgtc aaaatgcgcg atcattcccg gaagaatgac catcccgcga agcgcaaccg
     1141 caggtatttt taagatttct gtatccaaca tatcctctat ttttcttcta tggtctttaa
     1201 tttctgctcc tcaatggaaa gcttctcctc caccagatcc ttggtgatct cacactcggt
     1261 gatgctctca tccgacggaa tatggtacat cagatccagc gtcacagcct ccataatcgc
     1321 gcgcagtcct cgcgctcccg tcttgcgctc gagcgccttg tcagcgatcg cctccagtgc
     1381 gtccgcgtca aatgcaagct tcacgccatc cagctcaaac agcttctcgt actgtctggt
     1441 cagcgcattc ttcggctctt ttaagatgcg gatcagggcg tcgcggtcta acatatccaa
     1501 agataccgtc accggcacac ggccgatgaa ctccgggatc agaccgaact tcacaaaatc
     1561 ctgcggcagc gcatgcttca acacatcgcc gatattgtac tcattctttg acttgatgtc
     1621 cgcgttgaaa ccgattgcct tctgatccat acgcgtctca atgatcttct caagtccgtc
     1681 aaatgctcct ccgcagatga acagaatgtt cgtcgtatcg atctggatca gttcctgctg
     1741 cggatgcttt ctgcctccct gcggcggtac cgatgcaatc gttccctcaa ggatcttaag
     1801 cagcgcctgc tgtacaccct cgccggatac atctctcgtg atagacacgt tctcggactt
     1861 cttggagatc ttatcgatct catcgatata gatgataccg tgctgggcac gctcaatgtt
     1921 gtaatctgcc gcctgaataa tcttcaagag gatattctct acatcctcac cgacataacc
     1981 tgcctctgtc aacgttgtgg catctgcaat cgcaaacggc acatttaaga cacgcgccag
     2041 cgtctgcgcc agcagcgtct taccggaacc ggtcgggcca agcatcagga tgttgctctt
     2101 ctgcagttcc acgcccagat cgtcccctgc cataattctc ttgtaatggt tatatactgc
     2161 aacggaaaga acctttttcg cctgatcctg tccgatcaca taatcgtcca gaaaggcttt
     2221 taattcttcc ggttttaaaa ggttgatctg ctccgtctct tctgcggcgc cggtctcttc
     2281 ctcgttctca aactcttcct caataatctc tgcacagatg tccacacact catcacagat
     2341 atatgcccct ttcggtcctg cgatcagctt gcgcacctgt ccctgagact ttccgcaaaa
     2401 ggagcagcgg atctcatcgt cacttcttcc tgccattttt aactattctc catgttctat
     2461 cttacttcct gccaaaatca ggtcttattt gctttcttcc ttcttggtgt ccttgcggat
     2521 gatgacatcg tcgatcagtc cgtacgcctt tgcctcctcg gcagacatgt agtaatcgcg
     2581 ctcagtatcg gcagcaacca catcgtacgg cttaccggta ttggcagcga gaatctcatt
     2641 taactttttc ttggtcttta aaatgttctc cgctgcgatc tggatctctg tcgcctgtcc
     2701 tttggctccg ccggacggct gatggatcat gacctccgca ttcggaagcg catagcgctt
     2761 gccctttgtt cctcccgcga gcaggaatgc acccatgctt gctgcaagtc cgatacagat
     2821 ggtggacaca tcacacttga tgtactgcat tgtatcgtaa atcgccatac ccgctgtcac
     2881 cataccgccc ggggagttga tgtaaagctg gatgtccttg ttcggatctt cggactccaa
     2941 aaacagaagc tgtgccacaa caaggcttgc cgttgtctca ttgacttcct ctccaaggaa
     3001 aataatacga tcttttaaca ggcgggaata aatatcataa ttgcgttctc cgcggctggt
     3061 ctgctctacg acgtaaggta ctaaactcat attcgatcct ccattttata cttttttaat
     3121 aattacttat aagcttaaac agaccggccc aatagagcca gtctgtctgt ttagcaataa
     3181 gaactgacag gggaattact cctctgttcc ctctttctct tctgcttctt tttctttctt
     3241 ggattttgct ttcgctctct ccttcacgtt ctccatgatc aggtctactg ccttggtcac
     3301 tgcaaggtct ctcttcatgg attccttctc gttgtcgccg atgtactctt tgagcttgtc
     3361 agcttccata ccgtactgtg ctgccatctt ctcaatctca gcatcaagct cttcatcggt
     3421 aatctcaatc ttctcatcct ttgcaatctg ctctaatacc aggctgctct tgatgcgtgt
     3481 ctcagcttcc gggcgaacct gctcttttaa cttgtcgagt gtcagacc
//
LOCUS       DBMDQJ010000198         3520 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9750_length_3520_cov_1.680316, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000198 DBMDQJ010000000
VERSION     DBMDQJ010000198.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3520)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3520
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9750_length_3520_cov_1.680316"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..1363
                     /locus_tag="ACZO9R_05260"
     CDS             2..1363
                     /locus_tag="ACZO9R_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR0912506.1"
                     /translation="MNQFCEITPELRRLAAKSAECSKIDPELYTRYDVKRGLRDLNGK
                     GVLVGLTEISEVSSTKIVNGESVPADGELFYRGYNVKDLIAGLPEDSHFGFEECTYLL
                     LFGKLPKKHELRDFSALLSSYRTLPTSFVRDIIMKAPSKDMMNTLARSVLTLYSYDEM
                     ADDISLPNVLRQCLQLISLFPLLSVYGYQAYKHYHDGASLFIHSPKEEYSTAENILHI
                     LRPDSKFSPLEAKLLDIALILHMEHGGGNNSTFTTHLVSSSGTDTYSVIAASLGSLKG
                     PKHGGANIKVVRMFDDMKANLKDWTDEEEISRYLTALLHKEAFDHAGLIYGMGHAVYS
                     LSDPRAQIFRGFVKRLSAEKGYEKEFALYTAVERLAPQIIAAERKIYKGVSPNVDFFS
                     GFAYSMLGLPVELYTPIFAIARIAGWSAHRLEEVAGGGKIMRPAYKNVGEHKIYVPMD
                     ERV"
     gene            complement(1966..>3520)
                     /locus_tag="ACZO9R_05265"
     CDS             complement(1966..>3520)
                     /locus_tag="ACZO9R_05265"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912507.1"
                     /translation="EALELTSEVIAVLQSDSQNIEEQIDALAAQNNMIVFVTDTDGNT
                     IYSAEYIPNSRLCEMPQEMIQKLYEAAKSKGGSTKLEFEGDIGKNDFQNPQDVMNPDA
                     AADAAGAGNIPDAADVTGAGAENAPDAAAGATGAGNSPDAADVAGAGAENAPGAANGA
                     EQETAGMPAENGFMQNHGQERIESVIYVNVVSIDGNERILFVNAQLTPVDATVNTLRA
                     ELVWITVIMIVLSLGIALLISRKVSRSLIGLNASAKEMAGGNFDVHFDGTDYREVAEL
                     SDTLNMTARELGKSERLRRELIANVSHDLRTPLTMIIAYAEVMRDLPGENSPENVQVV
                     IDEAERLTNLVNDMLDISKLQAGVLEKNVTVYNLTESIQSVFARYNKLKEQDGYKIDF
                     SYDQMVMVEADEYKIFQVIYNLVNNAINYTGDDKQIWVRQIVREDGYVRIEVTDSGEG
                     IAPEALPYVWDRYYKVDKTHKRAVMGTGLGLSIVKNILELHDARYGVESEVGKGSTFW
                     FELKVYQIV"
BASE COUNT          801 a          996 c          827 g          896 t
ORIGIN      
        1 aatgaatcaa ttctgtgaaa ttacacccga actgcgtcgg ctggccgcaa agtctgccga
       61 atgttcaaaa atagacccgg aattatacac aagatacgat gtcaaacgcg gtctgcgcga
      121 tctgaacgga aaaggtgtcc tggtcggact gacggagatc tcggaagtca gttccacaaa
      181 aattgtcaat ggtgaatctg ttcccgcaga cggcgagctc ttctatcgtg gttacaatgt
      241 caaagatctg attgccggac ttcccgagga cagccacttc ggctttgagg aatgtaccta
      301 tctgctgctt ttcggcaagc ttcccaagaa gcatgagctg cgcgattttt cggctctgct
      361 gagcagttac cggacgctgc cgaccagctt tgtgcgtgac attattatga aggcgcccag
      421 caaggacatg atgaatacgc ttgcgcgcag tgtccttacc ttatattctt acgatgagat
      481 ggcggatgat atttcgcttc ccaatgttct cagacaatgt ctgcagttaa tcagtctttt
      541 cccgctttta tccgtgtacg gttatcaggc atacaagcat tatcatgacg gcgcgagcct
      601 gtttattcat tccccgaagg aagaatattc caccgccgaa aatattctgc acattttaag
      661 accggacagt aagttctcgc cgcttgaagc aaagcttttg gatattgccc tgattcttca
      721 catggaacac ggcggcggta acaactccac ctttaccacc cacctggtat cttcctccgg
      781 aacagataca tactccgtta ttgcggcatc gttaggttcc ctcaaaggtc ccaagcacgg
      841 cggtgccaac atcaaggtgg tgcgcatgtt cgacgatatg aaagccaatc tcaaggactg
      901 gacggatgag gaggagatca gccgttacct gactgccctg ctccacaagg aagcgttcga
      961 tcacgccggt ctgatctacg gtatgggaca cgcggtctat tcgctgtccg atccgcgtgc
     1021 acagatcttc cgcggattcg taaagcgtct ctccgcagaa aaaggctatg aaaaagaatt
     1081 cgcactctac acggcagtgg aacgtcttgc cccgcagatc attgccgccg agcgcaagat
     1141 ctataaaggt gtcagcccga atgtcgactt cttcagcgga ttcgcctaca gcatgctcgg
     1201 acttccggtc gaactctaca cgccgatttt cgccatcgcg cgaatcgccg gatggagtgc
     1261 gcaccgtctg gaggaagttg caggcggcgg caagatcatg cgtcctgcct acaaaaacgt
     1321 cggcgagcat aagatttatg tcccgatgga tgagcgtgtc tgattttcac tgcacgcctt
     1381 gttctaaaaa agccgccccg gactgacgga gcggacaaaa tggaatacta tggaaaatac
     1441 gcgagatgag agaactccgt ctgtaatgga cggggctctt ctttagagga aaatgcgagt
     1501 gtttctttca tgccgcgttt gacatcttat tctgtgtctt tgccacgttt gaacactccg
     1561 ccggctgtgt ccgacacgcc gcagacaatg agcgaccttc ctagtggcgc taatgccgct
     1621 gcttcacaga caatataaaa tcgcgcgaac cgaaactcgt tggcatctgc tattgaaaat
     1681 atggcagatg ccaattcaaa catcggcttc gcgcgattat gttttgttca gcagcggtat
     1741 aagcgccaca cgaaagctct aaaattgtct gtggcgtgcg gactcatccg gcggagtgct
     1801 ccaaacttgg aaagacacgg atcatcaggg atgtcaaacg cggtgtgatt agaaacaccg
     1861 gattttacga tcagaagaac cgccggccat tatagccgga gttctaagtt cgcatattgc
     1921 cacatgcttc ctcctgcccg ctccgtcaat ccgcgggcag ctcttttaaa caatctgata
     1981 tactttcagt tcaaaccaga aggtagaacc ttttcccact tcactctcta ctccgtagcg
     2041 tgcatcgtgc agttccagaa tattttttac gatcgaaagt ccgagtccgg ttcccatgac
     2101 cgcccgcttg tgtgtcttgt ccactttata gtagcggtcc cacacatacg gaagcgcctc
     2161 cggagcgatt ccctcgccgc tgtccgtcac ttcgatccgc acatatccgt cttcccgcac
     2221 aatctgacgc acccagatct gtttgtcatc cccggtgtaa ttgattgcgt tattgaccag
     2281 attgtagatg acctggaaga ttttgtactc gtccgcctcc accatgacca tctggtcgta
     2341 ggagaaatcg atcttataac catcctgctc ttttaactta ttataccgtg caaatacaga
     2401 ctgaatactc tcggtcaggt tgtagaccgt gacatttttt tccagtacgc ctgcctgaag
     2461 cttggagatg tctaacatat cattgacgag attggtcagc cgctccgcct cgtcaatgac
     2521 cacctgcaca ttttccggag aattttcccc cggcagatcc ctcatgacct ctgcgtaggc
     2581 aatgatcatc gtgagcggtg tccgcaggtc atgcgataca ttggcaatca gttcccgccg
     2641 caggcgctcg ctctttccaa gttcccgcgc agtcatattc agggtatcgg aaagttccgc
     2701 gacctcccgg taatcggttc cgtcaaaatg aacgtcaaaa ttgccgcctg ccatctcctt
     2761 cgcggaggca ttcagaccga tcagggaacg tgacactttt cttgaaatca aaagtgcaat
     2821 accgagcgag agaacaatca tgatgactgt gatccatacc agttcggcac gcagtgtatt
     2881 gaccgtcgca tccaccggcg tcagctgcgc gttcacaaac aggattctct catttccatc
     2941 gatcgagacg acgttcacgt aaatgacgct ctcaatccgt tcctgtccgt gattctgcat
     3001 aaaaccgttc tccgccggca ttcctgcagt ctcctgctct gcgccgttgg ctgcacctgg
     3061 ggcattttct gctcccgccc ctgccacatc ggctgcatcc ggggagtttc ccgctcctgt
     3121 ggcacccgct gccgcatccg gggcattttc tgctcccgct cctgtcacat cggctgcatc
     3181 cgggatattt ccggctcccg ccgcatcagc tgccgcatcg ggattcatca cgtcctgcgg
     3241 attctgaaaa tcattcttcc cgatatctcc ctcaaattcc agcttcgtgc tcccgccctt
     3301 gcttttggca gcttcgtaca gcttctgtat catctcctgc ggcatctcgc acagcctgga
     3361 attcgggata tattccgcgc tgtaaatggt attcccgtcc gtatccgtga caaacacgat
     3421 catgttattc tgcgccgcca gcgcatcgat ctgttcttca atgttctggg aatcgctctg
     3481 taagaccgca atcacttccg aggtgagttc cagtgcctcc
//
LOCUS       DBMDQJ010000199         3518 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9757_length_3518_cov_1.683933, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000199 DBMDQJ010000000
VERSION     DBMDQJ010000199.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3518)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9757_length_3518_cov_1.683933"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            108..2819
                     /locus_tag="ACZO9R_05270"
     CDS             108..2819
                     /locus_tag="ACZO9R_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR0912508.1"
                     /translation="MAEERELILKLGKKITDRIPYKLGMEKLTVNDPEYWGLAGVVTD
                     EMAEVALCMEVRKPITLPKLAKKTKKPEAELEKLLQEMAVVGIIEYNWENPQHEKQYI
                     LPMYVPGSAEFMVMNKEQVEEHTEIADFFENMSRLPLEKVTPMVPLGGAGIGMHVIPV
                     EKAIPAAQESADVEHISHWLKKYKDKYAIGQCSCRLQQRVRGEGTGDIEGEWCIGVGD
                     MADFLVQTGRGHYVELDEVLELLERAEKMGYVHQITNIDGEDKIFAICNCAVGVCNAL
                     RTSQLFNTPNMSRSAYRAHVTKENCVACGRCVEYCPTGAAKLGQKLCTKNGEITYPKQ
                     ELPDATKWGPEKWSTDYRDKNQINCYETGTAPCKTACPAHVPVQGYVKMAAEGRYMDA
                     LKLIKNENPFPAVCGSICNRRCEDACTRGTIDQAVAIDEIKKFIAEQEMHADKRYIPE
                     IVNHLGTGVDLPQKIAIVGGGPAGLSAAFYCRKMGYPVTVFEKESRPGGMLLNGIPSF
                     RLEKNIIDAEIEVLRQMGVTFKCGVEVGKDVTIQQLREEGFKAFYVAIGAQGGRKAGV
                     PGEDADGVKTGVEFLRSVNLDESTKLSGRTVVVGGGNVAVDVARAALRAGSGEVSMFC
                     LESRDIMPAAKDEVAEAEEEGISVNNSWGPKEILTENGKVKAIVFKKCLSVKDADGRF
                     NPQYDENDLMTVECENVLLSIGQSIVWGDLLKGTKVEIRPNGGAIADPVTYQTAEPDI
                     FVGGDVYTGPRFAIDAIAAGKEGFVSMHRFVNPGQSLTLGRNRREFIELDKNNIDVET
                     FDNAKRQIPGTKAGVAKETFRDLRCTLTEEQIKTEAKRCLGCGASVVDENKCIGCGIC
                     TTKCEFDAIHLTRDLPDASRMWKSEDKLKAIAPYALKRQFAIKRNKN"
     gene            2830..3174
                     /locus_tag="ACZO9R_05275"
     CDS             2830..3174
                     /locus_tag="ACZO9R_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HIR0912509.1"
                     /translation="MHELGIIVHIAKTLDQVAAENELKKIGSVTLDIGEVSGIVPEYL
                     TECWEFYRKKNPLIAECEMKIESLPAVTICEDCQKTYETVTYGRQCPHCGSYETYLVT
                     GNECSIKEIEAC"
BASE COUNT          971 a          758 c         1067 g          722 t
ORIGIN      
        1 cgcactctta gagtgcgcac tatttatgca aatataagac gaaaacagac gagacagaat
       61 aaaaaaacag gaaaaacaac gaaaaacgaa ggaaaaagga gactgttatg gcagaagaaa
      121 gagaactgat tttaaagctc gggaaaaaga ttaccgaccg tattccgtac aagcttggta
      181 tggagaagct cacggtaaac gatccggaat actggggtct tgcaggtgtg gttaccgatg
      241 agatggcgga agtggcactg tgcatggaag tgcgcaagcc gattacgctt ccaaagctgg
      301 caaagaagac aaagaagccg gaggcagagc ttgagaagct gctgcaggag atggcggttg
      361 tcggaatcat cgagtacaac tgggagaatc cgcagcatga gaagcagtac attctgccga
      421 tgtatgtacc gggaagcgct gagttcatgg taatgaacaa ggaacaggtg gaggaacata
      481 cagaaatcgc tgatttcttt gaaaatatgt caagacttcc gttagagaag gtgacaccga
      541 tggtaccgct cggcggagca ggtatcggta tgcatgttat cccggtagag aaggctattc
      601 cggcagcaca ggaatctgca gatgtggagc atatttccca ctggctgaaa aaatacaagg
      661 acaagtatgc gatcgggcag tgttcctgcc gtctgcagca gagagtccgc ggagagggaa
      721 ccggcgatat cgagggcgag tggtgcatcg gtgtcggcga tatggcagac ttcctggtac
      781 agaccggaag aggacactat gtggagcttg acgaagtgtt agagctttta gagcgcgccg
      841 agaagatggg ttatgtacat cagattacca atatcgacgg cgaggataag atttttgcaa
      901 tctgcaactg tgcagtcggc gtctgcaacg cgctgcgcac ctcccagctg ttcaatacac
      961 cgaacatgtc ccgctctgca taccgcgcac atgtgaccaa ggaaaactgt gtggcatgcg
     1021 gccgctgcgt ggagtattgt ccgaccggtg ctgcaaagct cggacagaag ctgtgcacaa
     1081 agaacggtga gatcacttat ccgaagcagg agcttccgga tgcgacaaag tggggaccgg
     1141 agaagtggag cacagattac cgtgacaaga accagatcaa ctgctatgag accggtacag
     1201 ctccgtgtaa gactgcctgt ccggcacacg ttccagtaca gggttatgtg aagatggcag
     1261 cggaaggcag atacatggat gccttaaagc tgatcaagaa cgagaatccg ttcccggcag
     1321 tctgtggttc tatctgtaac cgccgctgtg aggatgcatg taccagagga acgatcgatc
     1381 aggcggtagc catcgatgag atcaagaaat tcatcgcaga gcaggagatg catgcggata
     1441 agcgctatat cccggagatt gtcaaccatc ttggaaccgg cgtggatctg ccgcagaaga
     1501 ttgcgattgt cggcggcggc ccggctggtc tttccgcagc gttctactgc agaaagatgg
     1561 gttatccggt aacggtattc gagaaagagt ctcgtccggg cggtatgctg ttaaacggta
     1621 ttccgtcgtt ccgtctggag aaaaatatca ttgacgcaga gatcgaggtt ctccgccaga
     1681 tgggcgttac cttcaagtgc ggcgtagagg taggaaaaga cgtgacgatc cagcagctcc
     1741 gcgaggaggg ctttaaggca ttctacgtgg caatcggtgc acagggcgga cgcaaggccg
     1801 gagttccggg cgaggacgct gatggcgtga agaccggtgt ggaattctta agaagcgtga
     1861 acctggatga gagcacaaag ctttccggaa gaaccgtcgt tgtcggcggc ggtaacgtgg
     1921 cagtcgatgt ggcaagagca gcgctgcgcg caggttccgg tgaggtttcc atgttctgtc
     1981 tggagtcccg cgacatcatg ccggcggcaa aggatgaagt ggcagaggct gaggaagagg
     2041 gcatttccgt aaataattcc tggggtccga aggagattct gacagagaac ggaaaagtga
     2101 aggcaatcgt cttcaagaag tgtctctccg tcaaggatgc ggacggacgt ttcaacccgc
     2161 agtatgatga gaatgatctg atgaccgttg agtgcgagaa cgtgctgctt tccatcggac
     2221 agtccatcgt ctggggagat ctgttaaagg gaacaaaagt ggagatcaga ccgaacggcg
     2281 gagctatcgc tgacccggta acctatcaga ccgcagagcc ggatatcttt gtcggcggtg
     2341 atgtatacac aggaccgcgt tttgccatcg atgccattgc agcaggaaaa gaaggatttg
     2401 tatccatgca tcgttttgtc aatccgggac agagcttaac actcggacgt aaccgcagag
     2461 agttcatcga gctggataag aacaacatcg acgtggagac atttgacaac gcaaaacgcc
     2521 agattccggg cacaaaagcc ggcgtggcaa aggagacatt ccgcgacttg cgctgcacac
     2581 tcaccgagga gcagatcaag acagaggcaa agcgctgcct cggctgcgga gcttcggttg
     2641 tggatgagaa taagtgtatc ggctgtggaa tctgtacgac gaagtgtgag tttgatgcga
     2701 tccatctgac acgtgatctg ccggatgcaa gccgtatgtg gaagtctgag gacaaactga
     2761 aagcaattgc accgtatgcg ttaaagcgtc agtttgcgat caagcgcaac aagaattaag
     2821 aggagtcata tgcacgaact gggaattatt gttcatatcg caaagacgct ggatcaggtt
     2881 gcagcggaga acgaattaaa aaaaatagga tctgtcacgt tggacatcgg tgaggtcagc
     2941 ggtattgtgc cggaatatct gacggagtgc tgggaatttt accgcaaaaa gaatccgctg
     3001 atcgcagagt gcgagatgaa aattgagtcc cttccggcgg tcacaatctg tgaggactgt
     3061 cagaagacct atgagacggt tacttacgga agacagtgcc cgcattgtgg aagctatgaa
     3121 acgtatcttg tgaccggcaa cgaatgcagt atcaaggaga tcgaagcctg ctaaccggag
     3181 gcggagagcc ctataacaat cttaaaatgg acatggaggg aaagacacgg actgtttttc
     3241 cacccatgtc cgttttgcgt ctgtggaaaa gtgacagcta aatggctgcg tgtctgcata
     3301 aaataagaag aaggagtatg gcaggggagg agtttctcgt gccggaaaga aggagaattt
     3361 gaaaaatctg tatcagggag accggggaat cctggtatct tacatgcagc tggcgttaaa
     3421 gcgcgcgggg tatccgcttt tggtggacgg catctttgga acgaacacct gccgggcgct
     3481 cgctgatttt ttgcagaccg accgcacgcc ggaccgca
//
LOCUS       DBMDQJ010000200         3511 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9787_length_3511_cov_1.560704, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000200 DBMDQJ010000000
VERSION     DBMDQJ010000200.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9787_length_3511_cov_1.560704"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="ACZO9R_05280"
     CDS             complement(<1..545)
                     /locus_tag="ACZO9R_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR0912510.1"
                     /translation="MAMRIMIQGTMSNAGKSLIAAGLCRIFRQDGYRVAPFKSQNMAL
                     NSFITKEGLEMGRAQVMQAEAAGMEPTVAMNPILLKPTNDIGSQVIVNGEVIGNMSAR
                     DYFAYKKKLVPDILAAYHRLEEQADIIVIEGAGSPAEINLKENDIVNMGLAELLNAPV
                     LIAGDIDRGGVFAQLLGTQLLL"
     gene            complement(633..1355)
                     /locus_tag="ACZO9R_05285"
     CDS             complement(633..1355)
                     /locus_tag="ACZO9R_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HIR0912511.1"
                     /translation="MKKIFRKNQLVITALALMIAVAGYFSYMNNNMEDGQVTAEASAD
                     AGTVQDTYDISEEDMLAEQEIFTDEGTGEELALVEGTETAAVDAAQSGDVAQMTDGAA
                     DVENPGEAVLTSTTIANLDYAAEMKLNREQIRSKNKESLLEIVNNTTIDESLKQDAVN
                     KMIAMTDIAEREAAAEMLLEAKGFSDVVVSITEDNCDVVLNMGEVTDAKRAQVEDIVK
                     RKTNIAADKIVITPIVVDESGE"
     gene            complement(1352..2020)
                     /locus_tag="ACZO9R_05290"
     CDS             complement(1352..2020)
                     /locus_tag="ACZO9R_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HIR0912512.1"
                     /translation="MPEKLKRILQNKNWRSWKKSDWLLLALAGILLLVIALPVESGDG
                     TAEKAYNTAQGEKETSGGSGRTASGDSGGTASGSRTSEEGEQEYVQYLEKKLENILMQ
                     MDGVGRVDVMITVSDGGEQIVEKDKKTTSSSTGERDSGGGERSVTEQAGEDSTVYVET
                     ADEKYPYVQKETLPTVAGVVVVAEGGGNPAAVSEISESVQALLKVEPHRIRVVKMCSR
                     EESE"
     gene            complement(2021..2590)
                     /locus_tag="ACZO9R_05295"
     CDS             complement(2021..2590)
                     /locus_tag="ACZO9R_05295"
                     /inference="COORDINATES: protein motif:HMM:NF021121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HIR0912513.1"
                     /translation="MNAYMRGFVILFVLLVLLSYLPPGKQYRKYIRFYAQLLMVLAAL
                     RPLFVLFGGKDTFYEKMAYAQCMEKLSEFSRDTQKIAYLNQDRYRTEYENVIAENIRE
                     KVDDMLQEYGLGAESVTVELGEDHTMQEICIYITDENADEISVRRISVSDAAESGKNQ
                     EVCGKVKEELAAYYGMEDSGIRVSYVGGT"
     gene            complement(2602..>3511)
                     /locus_tag="ACZO9R_05300"
     CDS             complement(2602..>3511)
                     /locus_tag="ACZO9R_05300"
                     /inference="COORDINATES: protein motif:HMM:NF021088.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HIR0912514.1"
                     /translation="HWLCDALLYEIKTGKTILVEVVLLAAGFSMLKNFSGTFEKNYVS
                     DICFLLVYGVLCVLLLKSFEIYGTVAEEALNGSIDFMKALIPAFGISMVFSSGAESSA
                     AFYQLAFLVIYLVEWLFLSVLLPFIRVYVVTELFNHFFEDEKFQNFTELLAGVISWGM
                     KSAMMAVLGLNVMQGLLAPAKDRLLNGAVSRAAMMIPGVGNVLGGIGELMFGAGILIK
                     NCVGVTALIVLLAVGCIPVLKLACMSVLYKLAAAVAEPVADKRIVGCIKSMAQGGVLY
                     LKLVCYGIVLFFATIALQTAAVTSHA"
BASE COUNT          782 a         1159 c          664 g          906 t
ORIGIN      
        1 agaagaagct gtgtcccgag cagctgcgcg aacactccgc cccggtcaat atctccggcg
       61 atcagcaccg gcgcattcaa aagttccgcc agccccatgt tcacgatatc attctctttt
      121 aagttgatct ccgccgggct tcccgcacct tcgatcacaa tgatgtccgc ctgctcctca
      181 agcctgtggt acgctgcaag aatgtccggc accagttttt tcttataagc aaaataatcc
      241 ctggcactca tattcccgat cacttcgccg ttcacaatca cctgggaacc aatgtcgttg
      301 gttggcttta acaggatcgg attcatcgct accgtcggtt ccatccccgc cgcttccgcc
      361 tgcatcacct gcgcccgccc catctcaagc ccttcttttg tgataaagga atttaacgcc
      421 atattctgtg atttaaaggg tgccacccgg tagccgtcct gccggaaaat gcggcagagt
      481 cccgccgcga tcaggctctt tcccgcattc gacatggtcc cctgtatcat gatgcgcatt
      541 gccatagctt actccaatct aaaaaaatct ccatgcctca aatagcatgg agattatact
      601 acatatccgc ctcttttacc agtattttaa gcttattccc cgctctcatc caccacaatc
      661 ggggtaatga caatcttatc cgccgcaatg ttggtcttgc gttttacaat atcttccacc
      721 tgtgcccgct ttgcatccgt cacctctccc atatttaaga caacgtcgca gttatcttcc
      781 gtgatgctca ccaccacatc ggaaaatccc tttgcctcca gcagcatttc cgcagctgcc
      841 tcccgctcgg caatatccgt catggcgatc atcttgttga cagcatcctg ctttaaactc
      901 tcatcgatcg tcgtattgtt tacaatctca agcagggatt ccttattctt ggagcggatc
      961 tgctcgcggt ttaatttcat ctccgccgca taatcgaggt tcgcaatcgt cgtgcttgta
     1021 agcactgcct ccccaggatt ctcgacatcc gcggcaccgt ccgtcatctg tgccacatcc
     1081 ccggactgcg cggcatccac tgctgccgtc tccgttcctt ccacaagcgc cagttcctcc
     1141 ccggttccct catcggtaaa gatctcctgc tccgcaagca tatcctcctc cgatatgtcg
     1201 tatgtatcct gcaccgttcc cgcatccgcg ctcgcttccg ccgtcacctg gccatcttcc
     1261 atattgttgt tcatgtagct gaaatatcct gccaccgcaa tcataagtgc caatgctgtt
     1321 atcaccagct ggtttttccg aaatattttc ttcactccga ttcctccctg gaacacatct
     1381 taactactct aatcctatgc ggttccacct ttaataatgc ctgcacggac tctgaaatct
     1441 cagaaaccgc cgccgggttc ccgcctccct ctgcgaccac cacaacgcct gccaccgtcg
     1501 gcagcgtctc tttctgtaca tacggatatt tttcatctgc agtctccacg tataccgtcg
     1561 aatcttcccc cgcctgttct gtcacactgc gctcccctcc tccggaatca cgctcgcccg
     1621 tcgaggagga agttgtcttt ttgtccttct ccacaatctg ttcgcccccg tctgaaacgg
     1681 taatcatgac gtccactctg cccaccccgt ccatctgcat cagaatattc tcgagcttct
     1741 tctccagata ttggacatat tcctgctctc cctcctccga tgtccggctg cctgacgccg
     1801 tccctccact atctcctgac gccgttcttc cgctgccgcc tgacgtctcc ttttccccct
     1861 gtgccgtgtt atatgccttt tctgctgtgc catccccact ctccaccggc agtgcaatca
     1921 caagcaataa tatccccgcc agcgcgagca gcagccagtc gcttttcttc cagctgcgcc
     1981 agtttttgtt ctgtaagatc cgtttcagtt tttcaggcat ttacgttcct cccacataac
     2041 tcacccgtat tccggaatcc tccatcccgt agtacgccgc cagttcttcc tttactttcc
     2101 cgcagacctc ctggttcttc cctgactctg ccgcatctga cacactgatt cttctgaccg
     2161 aaatctcatc cgcgttctca tctgtgatgt aaatgcaaat ctcctgcatc gtatgatcct
     2221 ctcccagttc caccgtaacg ctctccgcgc caagcccgta ctcctgcagc atatcatcca
     2281 ccttctccct gatattttcc gcgatgacat tctcatactc tgtgcggtat ctgtcctgat
     2341 tcagataagc gattttctgc gtatcgcggg aaaattccga cagtttttcc atgcactggg
     2401 catatgccat tttttcataa aacgtatctt ttccgccaaa cagtacaaac agcggtctca
     2461 atgctgcaag caccatcaga agctgcgcgt aaaaacggat atatttccgg tattgtttcc
     2521 ccggcggcag ataacttagc agcaccagaa gcacaaacag aatgacaaat cctctcatat
     2581 atgcgttcat ggcacgtccc cctatgcatg tgatgtgact gccgccgtct gcagcgcaat
     2641 cgtagcaaaa aacagtacaa tgccatagca gaccagcttc agataaagca cgcctccctg
     2701 cgccatgctc ttaatgcatc cgacaatcct cttatccgcc accggctccg ccaccgccgc
     2761 cgcaagttta tagagaactg acatacaggc aagctttaga accgggatgc agcccaccgc
     2821 aagcagcacg atcagtgccg tcacccccac acagtttttg atcagtattc cggcgccgaa
     2881 cataagctcc ccgattcctc caagcacatt ccccaccccc ggaatcatca tcgccgcacg
     2941 gctgaccgct ccgtttaaaa ggcggtcctt tgccggtgca agcagtccct gcatgacgtt
     3001 taatccgagc accgccatca tcgcgctctt catcccccag gagatcactc cagccaataa
     3061 ttccgtaaaa ttctggaatt tttcatcctc aaaaaagtga ttgaacagct ccgtcaccac
     3121 atacacacgg atgaagggaa gcagcaccga aagaaagagc cactccacca gataaatgac
     3181 cagaaacgcc agctgataaa aggcggcgga gctctccgcc cccgatgaaa ataccatgct
     3241 gatgccaaac gccggaatga gtgccttcat aaaatcgatg ctgccgttta gcgcttcttc
     3301 cgctaccgtt ccatagattt caaaagactt tagcagcagc acacagagca cgccgtagac
     3361 cagcaggaaa cagatgtcgg aaacataatt tttctcaaag gttcccgaga aattcttaag
     3421 catggaaaaa cccgctgcaa gcagcaccac ctccaccaga attgttttgc cggtctttat
     3481 ctcatacagc agggcatcac acagccaatg t
//
LOCUS       DBMDQJ010000201         3494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9838_length_3494_cov_1.681108, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000201 DBMDQJ010000000
VERSION     DBMDQJ010000201.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9838_length_3494_cov_1.681108"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(31..1230)
                     /locus_tag="ACZO9R_05305"
     CDS             complement(31..1230)
                     /locus_tag="ACZO9R_05305"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HIR0912515.1"
                     /translation="MNVLVINCGSSSLKYQLIDSESEAVMAKGLCERIGIDGRLVYQK
                     TGLDKEITEAAMPTHKQAIQMVLDALVNPKTGAIASLSEIDAVGHRVVHGGEKFAAST
                     LLTPEVLKTIEECNDLAPLHNPANLIGIDACKELMPNVPMVGVFDTAFHQTMPKKAYL
                     YGLPHEYYEKYKVRRYGFHGTSHSFVSKRTAEFLGMDLKNSKIIVAHLGNGASISAVL
                     NGECVDTSMGLTPLEGLVMGTRSGDIDPAIMEFIAKKENLDIEGIMNVLNKKSGVQGM
                     SGLSSDFRDLQAGHEAGNELATNAIEVFSYRVAKYIGSYVAAMNGVDAIAFTAGIGEN
                     VNLVREKVMSYLGYLGVTVNQAVNDATHGDEAIISTADSKVKVCVIPTNEEIAICEET
                     VELVKGK"
     gene            complement(1424..2212)
                     /gene="srtB"
                     /locus_tag="ACZO9R_05310"
     CDS             complement(1424..2212)
                     /gene="srtB"
                     /locus_tag="ACZO9R_05310"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR0912516.1"
                     /translation="MKRNRNIGSILLIAAGLLLIAAALYELIPALLAYKRADDTYEAL
                     REAYVTGQPGTSSDDPAEEGEELPAWYEALQVDFETLKEANPDVTGWIYFDNIEQIDY
                     PILYSGDDETYLHTDLYGNASKSGCIFMEGNNVPDFNDCHTILYGHNMKNGSMFGSLK
                     QYKNDGFYEKNAYFTVFTEDAAYRYQIFAYSDVPEDSDVYTTGYLPDETFDGFLTELL
                     KSSYKDTGVTVGKDDKVLTLSTCSGDGLRFVVHAVRVDEHESGV"
     gene            2301..>3494
                     /locus_tag="ACZO9R_05315"
     CDS             2301..>3494
                     /locus_tag="ACZO9R_05315"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF017452.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(Met) cytidine acetate ligase"
                     /protein_id="HIR0912517.1"
                     /translation="MNVIGIIAEYNPFHNGHAYQIAHVRKNLHADYIVVATSGDYVQR
                     GEPALLDKYTRARMALSSGADVVLELPVLWSTASAELFADAGISLFEKTGCVNGICFG
                     AESGDLALLRRIADVLADEPADLKASLKHNLKSGSTFPKAREAALLSYFSGSAGQDGA
                     LPVSAETLSSLLASPNNILALEYLKALRRRASSITPYLLKREGAAYHETSILSGASAI
                     PASASAIRHTLFADAAGACGNSADGILRHAMPQEALAILQDYRADFPLLCADDFSGIL
                     GYLLLSSSATQLARTADSSPEFANRMRNQLPYYTSFCSFASRLKSKEMTLTRINRILL
                     HSILGITSSDYACGNALDKIPYLRILGFRESAAPLLAALKASAAVPLITRPSQAPKLL
                     SPDAMR"
BASE COUNT          772 a         1019 c          790 g          913 t
ORIGIN      
        1 agaattgtca actgcttttt gaattatttt ttattttcct tttactaact ctacggtctc
       61 ctcgcagatt gcgatttcct cgttggtcgg gatcacgcat acctttacct tggaatccgc
      121 ggtagagatg atcgcctcgt cgccgtgggt tgcatcgttc actgcctgat ttaccgtcac
      181 gccgagataa ccgagataag acattacctt ctcacgaacc aggttcacgt tctcaccgat
      241 acccgcggta aatgcgatgg catctacacc gttcattgct gcgacatagg agccgatgta
      301 ttttgccaca cgatagctga atacctcgat cgcattggtt gcaagctcgt tgcctgcctc
      361 gtgtcctgcc tgcaggtcac ggaagtcaga ggagagaccg gacattccct gtacgccgga
      421 cttcttattt aatacattca tgataccttc gatatcaaga ttctctttct ttgcgatgaa
      481 ttccatgatc gccggatcaa tatcaccgga acgtgttccc atgacaagtc cctcaagcgg
      541 tgtcagaccc atggaggtat ctacacactc accgtttaat accgcactga tggatgcgcc
      601 gttgccgaga tgtgccacga tgatcttgga atttttaaga tccatgccaa ggaactctgc
      661 ggtacgcttg gatacaaagc tgtggcttgt tccgtggaaa ccgtatcttc tgaccttgta
      721 tttctcataa tactcatgag gcagaccgta aagatatgcc ttcttcggca ttgtctgatg
      781 gaaagcggta tcgaatacgc caaccatcgg tacgttcggc atcagctcct tgcaggcatc
      841 gataccgatc aggtttgccg ggttgtgaag cggtgcaaga tcgttgcact cctcgattgt
      901 ctttaatact tccggtgtca gaagtgtaga tgctgcgaac ttctctccac cgtgtacgac
      961 acgatgtccg actgcgtcga tctcggaaag gcttgcgatt gcaccggtct tcggattcac
     1021 cagtgcatca agaaccatct ggattgcctg cttgtgcgtc ggcattgcag cttctgtaat
     1081 ctctttgtcc agtccggtct tctggtatac cagtcttccg tcgattccga ttctctcaca
     1141 gagacctttt gccattaccg cttcgctctc agaatcgatg agctgatatt ttaatgaaga
     1201 gcttccacag ttgataacta atacgttcat aatatcctcc ttgttttgtg aagcaaaatc
     1261 cgagccctct ggcgaggaga ggatctgcct ccttgttttg cgaaggtctg tctctcacag
     1321 accgcatata gttttagtat acgcttgtca cgcccgcgaa acaagccagt aattgccagt
     1381 ttctttgtac aaaatgaaaa acagcctgac aaaaatttaa cagtcacacc ccgctttcgt
     1441 gttcgtcaac ccgcaccgcg tgcaccacaa accgcagccc gtcacccgag caggtggaca
     1501 gtgtcagaac cttatcatcc tttcccaccg taacgcccgt atccttgtag gaggacttaa
     1561 gcagttctgt gaggaacccg tcaaaagtct catccggcag atatccggtc gtatagacat
     1621 ccgaatcttc cgggacatca ctgtacgcaa aaatctgata gcggtaggct gcatcctccg
     1681 taaacacggt aaaatacgca tttttttcat aaaagccgtc gtttttatat tgtttcagcg
     1741 aaccgaacat gcttccgttt ttcatgttgt gtccgtacag aatcgtgtgg cagtcgttaa
     1801 aatcggggac gttgttcccc tccataaaaa tgcagccgga tttcgacgca tttccataga
     1861 gatcggtatg caggtaggtc tcgtcatccc cggaatacag aatgggataa tcgatctgct
     1921 ctatattgtc aaaataaatc cagccggtca catccggatt tgcttccttt agcgtctcaa
     1981 aatccacctg caatgcctca taccatgccg gcagctcctc tccctcctcc gccggatcat
     2041 ccgaagatgt ccccggctgt ccggtcacat aagcctcgcg cagcgcctcg taggtatcgt
     2101 ccgcccgctt gtacgcgagc agcgccggga tcagttcata aagggctgcc gcaatcagca
     2161 aaagccctgc cgcaatcaga agaatcgatc caatgtttct gtttctcttc acatgaaatg
     2221 ctcctttttc tttttttcta tttcacagta tgctatgata gctacagttt ttattacatt
     2281 gcagtacggg aggatccgcc atgaacgtca ttggcattat cgcagaatac aacccattcc
     2341 acaacgggca cgcctatcag atcgcgcatg tcagaaaaaa tcttcacgcc gactatatcg
     2401 ttgtcgccac aagcggcgac tatgtacagc gcggagagcc cgcccttctg gacaaataca
     2461 cccgtgcccg catggcgctc tcctcgggtg cagatgtcgt gctcgagctt cccgtactct
     2521 ggtccaccgc ttccgcagag cttttcgcgg acgccggaat ctccctcttt gagaaaacgg
     2581 gctgcgtaaa cggcatctgt tttggcgctg agagcggcga ccttgccctc cttcgccgca
     2641 ttgcggacgt tctcgcggat gaaccggcgg atttaaaggc ttccctgaag cacaacctaa
     2701 aatccggcag tacattccca aaggcaaggg aagctgccct gctctcgtat ttttccggct
     2761 ccgccggtca ggatggcgcc ctgcccgtat ccgcagagac gctctcctcc ctgctcgcct
     2821 ccccgaacaa catactggct ctggaatact taaaagcgct gcgccgccgc gcgtcttcaa
     2881 tcacacccta tctgctaaag cgggagggtg ccgcgtacca cgaaacgtcc atcctctccg
     2941 gtgcatctgc catcccagcc tcggcatccg cgatccggca tacactcttc gccgacgctg
     3001 ccggagcatg tgggaattct gccgacggga ttctgcgtca tgccatgccg caggaagcac
     3061 tggccatcct gcaggactac cgggcagatt ttccgctgct gtgcgccgat gacttttccg
     3121 gcattctggg gtatctgttg ttaagcagtt ctgccacgca gctcgcccgg accgccgatt
     3181 cctccccgga gtttgccaac cgcatgcgaa accagcttcc ttattatacc tctttttgct
     3241 ccttcgcgtc acggttaaaa agcaaagaaa tgacgctcac gcgcatcaac cgcattttgc
     3301 tgcactccat ccttggcatt acttcttctg attacgcatg cggcaatgcc ttggataaaa
     3361 ttccctacct gcgcattctc ggctttcggg aaagcgccgc tcccctgctt gccgcactaa
     3421 aggcatccgc tgcagttcca cttatcacac ggccatcgca ggcaccaaag cttttgtccc
     3481 cggatgccat gcga
//
LOCUS       DBMDQJ010000202         3494 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9839_length_3494_cov_1.667551, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000202 DBMDQJ010000000
VERSION     DBMDQJ010000202.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9839_length_3494_cov_1.667551"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="ACZO9R_05320"
     CDS             complement(<1..1331)
                     /locus_tag="ACZO9R_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HIR0912518.1"
                     /translation="MGRRKYVLIVDDCQQDRMAVADVLRSDYDILEACNGKQALEILS
                     RKRAQISLIMLDLMMPVMDGYEFLEMYRKRKEYSYLPVVVCTTEDDPEREQKSLELGA
                     WDFVLKKSSPGIMRLRAGNAIEKSKVRFLEYDFLTGIYGQQKFYQATRELLDQRAGAN
                     FAFIHFDIDRFRIINTLYGSKEGDRLIHFVAGAIRKVMTAYGRGTYGRLGGDVFGMCV
                     PYEDGAAIYHILEGIRAEIRKHSVHYYLETCAGIYLVDDPDMEVAAMHDNAEIAAAQC
                     KGQYMVHDVLYTEEIGQKVLREQHIIDEMDAALAEQQFIVYFQPKYQLKKMAPYGAEA
                     LVRWKKPSGEIVLPNEFIPIFERNGFITKLDYYVWEKVCQFIDSELSQGRNPAPISVN
                     VSRVNLYNPDFMDSLIDLIHRYHIPPHYLNLELTESVFSEDAELIQRAVNYL"
     gene            complement(1401..2006)
                     /locus_tag="ACZO9R_05325"
     CDS             complement(1401..2006)
                     /locus_tag="ACZO9R_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HIR0912519.1"
                     /translation="MNYLWAFMILTGIIYGAFHGTLPQITTAALDSAKEAVTLCITMM
                     GVMSFWVGLMRIAEKAGIIEGLSRRMRPVLHFLFPDLPQEHPANEYIATNMIANVFGL
                     GWAATPAGLKAMEALQERNLELCGQKGTSRKRGPDIATDEMCTFLIVNISSLQLIPVN
                     IIAYRSQYGSVNPAAVVGPGLIATICSTAAAIIFCKLKKRC"
     gene            2243..>3494
                     /locus_tag="ACZO9R_05330"
     CDS             2243..>3494
                     /locus_tag="ACZO9R_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR0912520.1"
                     /translation="MTSVFLLNGCSRFSMQTDFVAGSTSSFDTFLDDFFRAEVSSNTI
                     NLHFSLSHPENYGITETPITLGSLSEEALAASHASLENTLAALAKYDRDALPPSGQLT
                     YDVLQNYLKTELSASDLTLYDEPLRPTTGIQSQLPVLYEEYRFRQPADVEDYLALLAL
                     TGDYFDQIIRFEQQKAQAGLFMSDYACNTLISQCNAFTADPDQHYLIQTFDHKIDAMS
                     ELTEDQKTLYKEKNAALVREHVLPAYTHLAAALTELLGSGKNDMGLCYFADGKDYYRY
                     LVCHNTGSASDLPALQDRILEKRQSDLQQAAALLSENPQLKASQTTVRLPAADPIATL
                     NGLQEAMRANFPAPPETTFTVSSIDECMEDYMAPAFYITSPIDDYAQNSIFINASTDT
                     SSLRYFTTLAHEGFPGHLYQTVMSY"
BASE COUNT          809 a         1056 c          757 g          872 t
ORIGIN      
        1 agataattca ccgctctctg gatcagttcc gcatcctcgg aaaagacgct ctccgtcagt
       61 tccaggttca gataatgcgg aggaatatga tagcggtgga tgaggtcaat cagggaatcc
      121 ataaaatccg ggttatagag gttgactctg gatacgttga ccgaaattgg cgccggattc
      181 cttccctggg aaagctcact gtctatgaac tggcagactt tctcccacac gtaatagtcc
      241 aacttcgtga taaagccgtt gcgctcaaag atcggtataa attcgttcgg aagcacgatc
      301 tcaccgctcg gcttcttcca gcgcaccagt gcctccgctc cgtacggagc catctttttc
      361 agctggtact tcggctggaa atagacgata aactgctgct ctgccagcgc ggcatccatc
      421 tcgtcaatga tatgctgttc gcggagcact ttctgtccga tctcctccgt atataaaaca
      481 tcgtgaacca tgtactgacc cttgcactgt gccgccgcaa tctcagcgtt gtcgtgcatc
      541 gccgccactt ccatatccgg atcgtccacc agataaatgc ccgcgcaggt ctccagataa
      601 taatgtacgg aatgtttccg gatctctgcc cggattcctt ccagtatgtg atagatcgcc
      661 gcaccatctt cgtaggggac acacattccg aacacatcgc cgccaaggcg tccgtatgtg
      721 cctctgccgt aagctgtcat gaccttgcgg atggctcccg ccacaaaatg gatcagccgg
      781 tctccctctt tggagccgta gagggtattg ataatccgga aacggtcaat gtcaaaatgg
      841 ataaaagcaa aattcgctcc cgcgcgctgg tctaaaagtt cgcgggtcgc ctggtagaac
      901 ttctgctgtc cgtaaatacc ggtaagaaaa tcatattcca gaaaccgcac cttgcttttc
      961 tcgatggcgt ttcctgcgcg cagtctcatg atcccggggc tggatttctt caaaacaaaa
     1021 tcccatgcac cgagctccag actcttctgc tctctctccg ggtcgtcttc tgtcgtacac
     1081 accacaaccg gaagatagct gtactccttc cgcttccgat acatctccaa aaattcataa
     1141 ccgtccatca caggcatcat cagatccagc ataatcagcg aaatctgcgc tctttttctg
     1201 gaaagaatct ccaacgcctg ctttccgtta catgcttcta ggatgtcgta atccgatctc
     1261 aaaacatccg ccactgccat ccggtcctgc tgacagtcat ctacgatcag tacatatttt
     1321 cttctaccca tacaagcccc cgtaccttga aacacgatct gcgtttctcc tatgtactat
     1381 tatagttttt tcgtttcctt tcagcaacgt ttttttaact tacaaaagat aattgccgcc
     1441 gcggtactac atatagtcgc aataagtccc ggacccacta ctgctgcggg attgacgctc
     1501 ccgtactggc tccggtacgc gatgatattg accggaatca gctgcaggga cgagatgttg
     1561 acgatcagaa acgtgcacat ctcatccgtg gcaatatccg gtccgcgttt ccgcgaggtt
     1621 cctttttgtc cgcagagttc caggttacgc tcctgcaatg cctccatcgc ttttaatccc
     1681 gccggcgttg ccgcccagcc aagtccgaag acatttgcaa tcatattcgt cgcaatgtat
     1741 tcgttggcgg ggtgctcctg cgggagatcc ggaaacagga aatgcagcac cgggcgcatt
     1801 ctccgtgaaa gtccctcaat gatccccgcc ttttccgcaa tccgcatcag tcccacccag
     1861 aaagacatca ctcccatcat ggtaatacaa agcgtaaccg cctcttttgc cgaatcgagc
     1921 gccgccgtcg taatctgcgg cagggttccg tggaacgccc cgtagatgat gcccgtcaga
     1981 atcataaatg cccagagata attcatatcc cctcacccgt ttttatcgtt tctccatata
     2041 tatgcatccc ggaataaatc ttgacagcac ccccttgatg cgcaaaaata gcgcagaact
     2101 tttgaataat tgcacatttt cccacataga atatgttaat tttccgacac aggagttttc
     2161 tcctctgccg cgtttccgag gtgtttgttt gcattcttcg tttatccaca cattttccag
     2221 gcaattcgtc ctttttccac tgatgacgtc cgtttttctg ctcaacggat gctcccgctt
     2281 ctccatgcag acggatttcg tcgccggttc cacgtcctcc ttcgatacgt ttcttgatga
     2341 ttttttccgg gcggaggtct cctccaacac gatcaatctt catttttcac tgtcccatcc
     2401 ggaaaactac ggcattacag agactccgat cacgctcggc agtctctcgg aagaagcact
     2461 tgccgcatcc catgcatctc tggagaacac gctcgcggct ctggcaaaat acgaccgcga
     2521 tgcccttccg ccctccggtc agctcaccta tgacgtcttg cagaattatc ttaagacgga
     2581 gctctccgca tccgatctga ctctctacga cgagccgctg cgccctacga ccggcattca
     2641 gtcgcagctt cccgtactat atgaggaata ccgtttccgg cagccagccg atgtggagga
     2701 ctatctcgcg ctcctcgccc tgaccggcga ttatttcgac cagatcatcc gcttcgagca
     2761 gcagaaagca caggccgggc tttttatgtc cgactatgcc tgcaacacac tgatttcaca
     2821 gtgtaacgcc ttcacagctg acccggatca acactatctc atccagacct tcgatcacaa
     2881 aatcgatgcc atgtcagaat tgacggaaga ccaaaagaca ctctacaagg aaaaaaatgc
     2941 cgctctggtg cgggaacatg tgcttcccgc ctacacccat cttgccgcgg cgctcacaga
     3001 gcttctcggc agcggcaaaa acgatatggg gctctgttac tttgcagacg gaaaagatta
     3061 ctaccgctac ctggtctgcc acaacaccgg gagcgcttcc gatcttccgg cgctgcaaga
     3121 ccggatctta gaaaaacggc aaagcgacct gcagcaggcg gcagcactcc tctccgaaaa
     3181 cccgcagtta aaagcatccc agacgaccgt ccggcttcct gccgccgatc ccattgcaac
     3241 gctaaacggg ctgcaggagg cgatgcgcgc aaattttcct gcaccacccg agaccacatt
     3301 tactgtaagt tctattgacg agtgcatgga agactatatg gcgcctgcgt tttacatcac
     3361 ctcgcccatt gacgattatg cgcagaactc catatttatc aatgcctcca ccgacacctc
     3421 ctccctgcgg tatttcacaa cgctggcgca cgagggcttc cccggccacc tttatcagac
     3481 ggtgatgtcc tatg
//
LOCUS       DBMDQJ010000203         3491 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9848_length_3491_cov_1.835398, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000203 DBMDQJ010000000
VERSION     DBMDQJ010000203.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3491)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3491)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3491
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9848_length_3491_cov_1.835398"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="ACZO9R_05335"
     CDS             <1..936
                     /locus_tag="ACZO9R_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912521.1"
                     /translation="FVSMIIYAIMRCMPSSFVEQQALTLSQRPGAKSYDEWVAQLNAS
                     YGLDTGVIQGYFRWAAKAIRGQFGDSWYFNQPVLTKFASVIWYSFALSLASFVLEIII
                     AIPAGIAAARKQYSATDYTITVIALVGISLPSFFVATLLKWVFSVKLGWFDLYGIVGR
                     NYQSLSSGQQILDMAWHLVLPVITLTIVSIGSLMRYTRTNMLEVLNADYIRTARAKGL
                     PEKRVIHYHAFRNILIPIITLLGGTLPGLFSGAMVTETLFQIPGIGYTSYQAVLQGDI
                     PFVMFYMLFSAVLILLGNLIADILYAVVDPRVRVN"
     gene            952..2616
                     /locus_tag="ACZO9R_05340"
     CDS             952..2616
                     /locus_tag="ACZO9R_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912522.1"
                     /translation="MENEKNNMKLDDAQRVRVLSPGMMVAKRFFRNRLAIVGLVIIVC
                     MFLFAFVGGAVNPYSQSEVFYRTEEMKKDYAGAAKIDEFQVFVADGVELPSDVYSKMI
                     LAVTKNALVFETRDGQTLALGRIDDDTYHIYAAEQQMNPLALKSEEHDAAEAAGQNQF
                     TYNGVEYVFDASGVKETLYTTTELGIACRKSVTAFDEGTTFSYDFYSKVLTAIANGDK
                     NIEADGVNYTVENEGSAAMIHTADGADYAYVSDMSMNPVASGIVLTPAFKEAAEAAMT
                     AGEDSFEYTDEQGETDTYLIKDKNGQYTIQRYQETRVIDSYAAPSVKHWCGTDGNGMD
                     LLARLMYGGRISLMIGFVVVIIELIIGIIVGGIAGFFGGWVDTLLMRVVDVVYCIPAI
                     PLYLILGSIMDYYKASAKTRIYMLCIVMSIIGWVSIARIIRGQILSLREQEFMTAAEA
                     TGIRVSRRIFRHLIPNVIPQLVVFASMGIGDVILAESTLSFLGLGIKYPAASWGSIIN
                     AVNDSYVMTNYLFVWVPAGVLILLTVLAYNFIGDGLRDAFDPKMKR"
     gene            2626..>3491
                     /locus_tag="ACZO9R_05345"
     CDS             2626..>3491
                     /locus_tag="ACZO9R_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912523.1"
                     /translation="MAFNNKKKNANYISAKESRAIARENRKITQEIEKKRNRKHIPEE
                     EYVTKMKNPENCVEFDNVQTYFFTDIGTVKSVDGVSFDVPQGKTVGIVGESGCGKSVT
                     SLSLMQLVQRPSGQTVGGEIRFNTGDHVYNVVNTPTSVMQKLRGNYMSMIFQEPMTSL
                     NPVLRIGEQVDEVIALHNPDMTKDDVKTRTIQMLEMVGIANKEGVYRMYPHELSGGMR
                     QRIMIAMALACNPRLIIADEPTTALDVTIQAQILDLLRGLKDKINSSIMLITHDLGVV
                     AEMADYVVVMYAG"
BASE COUNT          843 a          798 c          971 g          879 t
ORIGIN      
        1 ttcgtctcca tgatcatcta cgcgatcatg cgttgcatgc cgtcgtcatt cgtggagcag
       61 caggcactga cattgtcaca gagaccggga gcaaagagtt atgacgaatg ggtggcacag
      121 ttaaatgcat cgtacggtct ggataccggt gtgatccagg gatatttccg ctgggcagca
      181 aaagcaatcc gcggacagtt cggtgattcc tggtatttta accagccggt actgaccaaa
      241 ttcgcaagtg ttatctggta ttcgtttgca ctgtcactgg catcttttgt tctggagatc
      301 atcatcgcaa ttccggcagg tattgccgct gcgagaaagc agtattctgc aaccgattat
      361 acgatcaccg ttattgcact ggtcggtatt tctcttccga gctttttcgt tgcaacactt
      421 ttaaaatggg tcttttccgt aaagcttggc tggttcgacc tctacggtat tgtcggacgt
      481 aactatcaga gcctctcttc cggacagcag attctggata tggcatggca tctggtgctt
      541 ccggtaatca cactgacgat cgtgagcatc ggttccctga tgcgttacac cagaaccaac
      601 atgctcgagg tgctgaatgc ggattatatc cgtacagcgc gtgcgaaggg actgccggag
      661 aagcgcgtca ttcattacca cgcattccgc aatatcttaa tcccgatcat cacactgctc
      721 ggcggtacat tgccgggact cttcagtgga gccatggtaa cagagactct gttccagatc
      781 ccgggtatcg gatatacctc ttaccaggcg gtattacagg gtgacattcc gtttgttatg
      841 ttctacatgc tgttttccgc ggtactgatc ttacttggca acctgattgc cgatattcta
      901 tatgcagttg tggacccgcg tgtccgcgtg aactagaaag gaggagagaa catggagaac
      961 gagaagaata atatgaagct tgatgacgcg cagcgtgtcc gcgtactttc ccccggaatg
     1021 atggttgcaa agcggttttt ccgcaacaga ctggcaatcg taggactggt tatcattgtc
     1081 tgtatgttcc tgtttgcctt tgtcggaggt gcggtcaatc cgtattccca gagtgaggtg
     1141 ttctaccgca cggaagagat gaagaaggac tacgccggtg cggcaaaaat tgacgagttc
     1201 caggtgttcg tggcggacgg cgtggagctt ccgagcgatg tgtattccaa gatgatcctt
     1261 gccgtgacga aaaatgcgct ggtgttcgag accagagacg gacagacact ggcacttggc
     1321 aggattgatg acgacacata ccacatttac gcggcagagc agcagatgaa tccattggca
     1381 ttaaagtctg aggagcacga cgctgcggag gctgccggac agaaccagtt tacttacaac
     1441 ggcgtggagt atgtgtttga tgcatccggt gtgaaagaga cactgtacac gacgacagag
     1501 cttggtattg cctgccggaa gtctgtgaca gcttttgatg aaggcacaac attttcttat
     1561 gatttttaca gcaaggtgct gactgcgatt gcaaacggcg ataagaacat cgaggcggac
     1621 ggcgtgaact acacggttga aaacgaggga agcgctgcca tgattcatac ggcagacggt
     1681 gcggattatg cttatgtatc tgatatgagc atgaatccgg tagccagcgg tatcgtgctg
     1741 acaccggcgt tcaaggaggc ggctgaggct gcaatgactg ccggggagga ttcctttgag
     1801 tatacagacg agcagggtga gaccgatacc tacctgatca aggataagaa cggtcagtac
     1861 acgatccagc gctatcagga gacgcgtgtc atcgacagtt atgcggcacc gtctgtaaag
     1921 cactggtgtg gaacggacgg caacggaatg gatcttctgg cgcgtctgat gtacggcgga
     1981 cgaatttccc ttatgatcgg atttgtcgta gttatcattg agcttatcat tggtatcatt
     2041 gtaggtggta tcgcaggatt tttcggcgga tgggtggaca cactcctgat gcgtgtggtt
     2101 gatgttgttt actgtatccc ggcgattccg ctgtatctga tccttggttc gatcatggat
     2161 tactacaagg cttcggcaaa gacacgtatc tacatgctgt gtattgtcat gagtatcatc
     2221 ggctgggtca gcattgcccg tattatccgc ggacagatct tgtccctgcg agagcaggag
     2281 ttcatgacgg cggcagaggc aaccggtatc cgcgtaagcc gtaggatttt cagacatctg
     2341 atcccgaatg ttatcccgca gcttgtcgta ttcgcaagta tgggaatcgg tgatgtcatt
     2401 cttgccgagt ccacattgag tttcctgggt cttggtatca agtatccggc ggcttcctgg
     2461 ggaagtatca tcaatgcggt caacgactct tacgttatga cgaactattt attcgtgtgg
     2521 gttccggcag gcgtcctgat cctgctcacg gtacttgcat ataactttat cggtgatggt
     2581 ctgcgtgatg cattcgatcc gaagatgaaa cggtaggtga caggaatggc atttaataat
     2641 aagaagaaaa acgcaaatta tatctccgca aaagaatccc gcgcgattgc gcgggagaac
     2701 cgcaagatta cgcaggagat cgagaaaaaa agaaacagaa agcatattcc ggaggaggaa
     2761 tatgtgacaa agatgaagaa tccggagaac tgcgttgagt tcgacaacgt tcagacttat
     2821 ttcttcacgg acatcggaac ggtcaagtct gtcgacggtg tatcgtttga tgtgccgcag
     2881 ggcaagacgg tcggtatcgt aggtgagtcc ggttgtggaa aatccgtgac aagcctttcc
     2941 ctgatgcagc tggtacagcg cccatccggt cagaccgtgg gaggagagat ccgctttaat
     3001 accggcgatc atgtatacaa tgtggtcaac accccgactt ctgtgatgca gaagctgcgc
     3061 ggcaactaca tgtccatgat tttccaggag ccgatgactt ccctgaatcc ggtacttcgt
     3121 atcggcgagc aggtggatga ggtcattgca cttcacaatc ctgacatgac aaaggatgac
     3181 gtcaagacac gtacgatcca gatgcttgag atggtcggca ttgccaataa ggaaggtgta
     3241 taccggatgt atccgcacga gctttccggc ggtatgcgcc agcgaatcat gatcgcaatg
     3301 gcgctcgcat gtaatccgcg tctgattatc gccgatgagc ctacaacagc gctcgatgtg
     3361 acgattcagg cacagatcct cgatctgctc cgcggactca aggataagat caacagttcc
     3421 atcatgctga tcacacacga tctcggtgtg gttgcagaga tggcagatta cgtggtcgtt
     3481 atgtacgcgg g
//
LOCUS       DBMDQJ010000204         3489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9855_length_3489_cov_1.383707, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000204 DBMDQJ010000000
VERSION     DBMDQJ010000204.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3489
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9855_length_3489_cov_1.383707"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="ACZO9R_05350"
     CDS             complement(<1..578)
                     /locus_tag="ACZO9R_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912524.1"
                     /translation="MTDVLFYQNYTYVADISAIVLCIICWLLLHSSYTVREKSLIIFK
                     AGTVCLMMATVCSISFHTAITRITESRKILIYVLQDGIYLLLILTFVLLCNYFGTLLK
                     LEERPARLLGAVSWGGYAVFAFLEIGSPFTHLGFYIDADLMVHQNFYFDPFRFAYIYY
                     CIVLVVVLFTYRKKFVLKMLRCLAYILLFSFG"
     gene            complement(734..2062)
                     /locus_tag="ACZO9R_05355"
     CDS             complement(734..2062)
                     /locus_tag="ACZO9R_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912525.1"
                     /translation="MFFENIKEAIISILSNKMRSLLTMLGIIIGISSVIIITTIGGSI
                     QSTLTATMNSLGGNTVSGYVEARYPEDDADWDTWIYPDMTQSDYVSQEMIDELVAQYP
                     DEIQGIAAENYLGGGQIKDEADSDNYANVSVDGMTPEQFEYMKLEMQAGRNLTRADNL
                     GKKRVCVVADTMAKNYFGDKDPIGETVSVTMDDGSIYDFVVVGVYKYNAALFGKVDTS
                     VREKDRSTYMMIPIQTSFKLQGADKTGYEYFNLLLTPLADVNQATEDIQNYFDEVYQN
                     NTNFHVTASNMQSSMGMINTVLNVITIAVSGIAAISLIVGGVGVMNIMLVSITERTRE
                     IGVRMALGAKRRTIRMQFVIEAIMLCLIGGIIGILIGVGVGALLGQAAKFVIANMYSE
                     YSNYIIMQVHPSATAILLSLFFSMLTGVFFGYYPANKASKMEVIDALRYE"
     gene            complement(2062..2742)
                     /locus_tag="ACZO9R_05360"
     CDS             complement(2062..2742)
                     /locus_tag="ACZO9R_05360"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912526.1"
                     /translation="MEKIVKTYYVGKPNELEILHGIDLTVNQGEFVSIVGESGSGKST
                     LMNIIGVLDRQTSGSYYLEGQDVNGMTDEVRSAVRNRRIGFVFQNFNLLPRANALKNV
                     MVPLIYGEEKTKNAKERAMEMLAMVGMDGRADHRPNELSGGQKQRVAIARAMINDPAI
                     ILADEPTGALDSKTGHMVMDLFHRLHEEQGKTIVLITHSQELATETQRIVTLLDGEIV
                     GDNRGGEH"
     gene            complement(2763..>3489)
                     /locus_tag="ACZO9R_05365"
     CDS             complement(2763..>3489)
                     /locus_tag="ACZO9R_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR0912527.1"
                     /translation="DTDTTSKDTLSNLKEQLADCEITAPCGGVVTAVNSKVGDINAEK
                     NVIMTIEDTSSLKMVATVQEADVLKLQEGMEATVTADATGDEQIKGTVTRVVRVKSQG
                     TTGSDGSTTSAGGYSIEIALDNQELLIGMAVKAKVMIQAKGEVLAVPYDLIQYDDNGQ
                     AYVLVAEADDGGNATAVRRNITVGDEVDYYTEVTGGDLAEGDMLIYDTTFSIAEGQTF
                     TPEQMYSEQDMGLTDGTGTEVSE"
BASE COUNT          870 a          985 c          709 g          925 t
ORIGIN      
        1 atgccaaacg aaaacagtaa aatataggca aggcagcgca gcattttcag cacgaacttc
       61 ttccggtacg taaaaagcac aacgacaaga acgatacagt aataaatata ggcaaaacga
      121 aacggatcaa aatagaaatt ctgatgaacc atcagatctg catcgatata aaaacccaga
      181 tgcgtaaacg gtgatccgat ctcaagaaaa gcaaacaccg catagccgcc ccaggatacc
      241 gcgcccaaga gtcttgccgg tctctcttcc agcttaagaa gtgtaccgaa gtaattgcag
      301 agcagcacaa acgtgagaat cagtaaaaga tatatcccat cctgtaacac ataaataaga
      361 atcttccggg attctgtaat ccgtgtgatt gctgtatgga aactgatact gcagaccgtc
      421 gccatcatca ggcaaaccgt tcccgctttg aagataatca ggctcttctc tcttaccgta
      481 taggaagaat gcagcagcag ccagcagatg atgcagagca caatcgcgct gatatcggct
      541 acataagtat aattctgata gaacaaaaca tccgtcataa actcattctc cccttcgatc
      601 attttccctt tatattatca ttatacattt attgacagca gatttcaatt ttttcacgca
      661 aaatgacagc aaaggggcag agaagcggta aagcttctct gccccttttt ctgtcatctt
      721 tggctgcaca cgcctattcg tagcggagtg catcgataac ttccatcttt gacgccttgt
      781 tcgccggata ataaccgaag aacactcctg tcagcatcga gaagaacagt gacagtaaaa
      841 ttgcagttgc cgatggatgc acctgcataa taatgtaatt ggaatactcg gaatacatat
      901 tggcaatcac aaacttcgct gcctggccga gcagtgctcc gacaccaaca ccgatcagaa
      961 taccgataat gccgccgatt aagcagagca tgatcgcctc aatgacaaac tgcatacgga
     1021 tcgtccgccg ctttgcgccg agtgccatac gcacaccgat ctccctcgtc cgctccgtga
     1081 tcgacacaag catgatattc atgacaccga caccgccgac gatcagcgag attgctgcga
     1141 ttccggatac tgcaatcgtg atgacattga gcaccgtgtt gatcatgccc atgctggact
     1201 gcatgttgct cgctgtcaca tggaaattcg tattgttctg atatacctca tcaaaataat
     1261 tctggatatc ttccgttgcc tggttgacat ccgcaagggg cgtcaggagc agattaaagt
     1321 attcataacc agtcttatct gccccctgca gcttgaagct ggtctggatc ggaatcatca
     1381 tataagtgga acgatccttt tctctgacag atgtatccac ttttccaaac agtgccgcat
     1441 tgtatttgta aacacctaca acaacaaaat catagatcga tccgtcgtcc attgttaccg
     1501 ataccgtctc gccaatcggg tctttgtctc caaaataatt ctttgccatc gtatccgcaa
     1561 caacgcagac tcttttcttc cccagattgt ctgccctggt aagattacgt cccgcctgca
     1621 tctcaagctt catatactcg aactgttctg gtgtcatgcc atccacactg acattggcat
     1681 aattgtcgga atccgcctca tccttgatct gcccgcctcc aagataattc tcggcagcga
     1741 taccctgaat ttcatccgga tactgcgcaa ccagttcatc gatcatctcc tgtgacacat
     1801 aatcactctg tgtcatatcc ggataaatcc aggtatccca gtctgcatcg tcctccggat
     1861 agcgcgcctc cacatagccg gaaactgtgt tgccgccgag ggaattcatt gtcgcggtca
     1921 gggtgctctg tatggaaccg ccgatcgtcg tgatgatgat aacggaactg ataccgataa
     1981 taataccgag catcgtgagc agtgaacgca tcttgttcga cagaatactg atgattgcct
     2041 cttttatgtt ttcaaaaaac atcagtgctc acctcccctg ttgtccccca cgatctcacc
     2101 gtctaagagg gtaacaatac gctgtgtctc cgtcgcaagc tcctgggagt gtgtgatgag
     2161 tacaatcgtt ttcccctgct cttcatgtaa ccgatgaaac agatccatga ccatatgtcc
     2221 ggtcttggaa tccagcgctc cggtcggctc atccgccaga atgattgcag gatcattgat
     2281 catcgcgcgg gcgatcgcca cacgctgttt ctgtccgccg gagagctcat ttggtctgtg
     2341 atctgcacgc ccgtccattc ccaccatcgc cagcatctcc atcgcacgct ccttcgcatt
     2401 cttcgtcttt tcctcaccat atataagcgg caccatgaca tttttcaatg cattggctct
     2461 cggcagcaga ttaaagttct gaaacacaaa gccgattctg cggttgcgga ccgcactccg
     2521 cacctcgtcc gtcatgccgt tgacgtcctg tccttccaga taatagctgc cggaggtctg
     2581 tctatccaat actccaatga tattcatcaa tgtacttttt ccggaacccg actcaccgac
     2641 gatggaaacg aactctcctt ggttcacggt aagatcgatt ccgtgcagga tttccagttc
     2701 attgggctta cctacataat atgtctttac aattttttcc attcggatta ctggttcact
     2761 cactattcgc taacctccgt acctgtaccg tctgtcagtc ccatatcctg ttcagaatac
     2821 atctgctccg gtgtaaatgt ctgtccctcg gctatcgaaa atgtcgtgtc gtagatcagc
     2881 atatctccct ctgcgagatc accgccggtc acttctgtat aataatccac ctcatcgccg
     2941 accgtgatat tcctgcgcac tgcggttgcg tttccgccat cgtccgcctc agccacaaga
     3001 acatacgcct gcccgttgtc atcatactgg atcagatcat acggaactgc cagcacttct
     3061 ccctttgcct ggatcatgac ttttgccttc acagccattc cgattaaaag ttcctgattg
     3121 tcgagcgcga tctcaatgga ataaccaccg gcagaagtcg tactgccgtc gcttcccgtc
     3181 gtgccctgac tcttcacacg gaccactctg gtcacggttc ccttgatctg ctcatcaccc
     3241 gtcgcatctg cggtcacagt tgcctccatg ccttcctgaa gctttaatac atccgcctcc
     3301 tgcacggttg caaccatctt taaggagctc gtatcctcaa tcgtcatgat gacgttcttc
     3361 tctgcattga tgtctccgac cttggaattc accgctgtta ccacaccgcc gcacggcgct
     3421 gtgatctcac agtctgccag ctgttctttc agattgctga gtgtgtcctt ggatgtcgtg
     3481 tcggtgtct
//
LOCUS       DBMDQJ010000205         3471 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9905_length_3471_cov_1.613353, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000205 DBMDQJ010000000
VERSION     DBMDQJ010000205.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3471)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3471)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3471
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9905_length_3471_cov_1.613353"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(292..627)
                     /locus_tag="ACZO9R_05370"
     CDS             complement(292..627)
                     /locus_tag="ACZO9R_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912528.1"
                     /translation="MKYIIEVFSVMLVLVFNLLLCISMLGVSADVAAAKEFKAAIIAE
                     IENSDFNPYVIDGCKAQAGTEGYELEVQSYTYGGQSDRRIAGINLYYRYRIPLLGVSQ
                     QRVTRGIAR"
     gene            complement(628..2163)
                     /locus_tag="ACZO9R_05375"
     CDS             complement(628..2163)
                     /locus_tag="ACZO9R_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912529.1"
                     /translation="MKREGLWEKLRLLNPKNLQREVHVYGYRFSWRTHLMAVIAALVG
                     IGGIGMVFQLKPLFLAGVLLTVLFVFPVLVLDMYKKMYEQKRFGDACAYMEQLLYAFQ
                     KTGKIVSALKEVRGIFGEGQIRLCVEEAIAHMEYGHPVGEQGVLREGLQKIERYYACD
                     KLATVHELLLNTEEYGGDVEASVTLVLEDIERFKKRGYQLQAEKRKSHTDNVISIVVA
                     VLLCAAALYLLNEMQEMFTSEVDVSVFTVPVIQISSMVFLLLLLAFYVKSVRSLTADW
                     LEEKPLYDTAYIRHSYELVMGYEKKAQQYRRLIPAGIVTMTAFVFWTVGWRITGILLL
                     VTGLLVAMQPRISYRIAERDVTAELYQALPRWLFEMVLLLQNNNVQMAITKSAQHAPA
                     VLGSELAELCARMDERPDQLQTYTDFCKKFDLPEMLSCMKMLHAFSENGTGDIDVQMN
                     HLIERVVLMQERADVLRSEERAFRMKLIFAYPVLAATGKLLADLTVGMALMMQVLGGM
                     GGA"
     gene            complement(2164..3228)
                     /locus_tag="ACZO9R_05380"
     CDS             complement(2164..3228)
                     /locus_tag="ACZO9R_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR0912530.1"
                     /translation="MGEIMTFEPTEAQFGVFWKYLQRPNVTDVDYNGSELWITDLKIG
                     RYRVKEHLSDAFISTFTHNISNCVNRQFNNANKVLEADTRELRISIIHEAAAASGTSI
                     CIRKSPSMVRMNIERMLEESYCPERILQLLMNCVLAGMNFVFGGEPGAGKTECAKFFM
                     QFIPREERVITIEDSLEIHYRDINPGADAVELRVGEDFSYTDAIKASLRQNPKWLVLS
                     EARSVEVTSLLEQWSTGVSGFTTIHLDDVRKLPDRIQNMMSDARDAERMENRIYRYVN
                     VGILIRCVRQPDGKIRRYMDQLCFYSREQYENRIYMILEDGELLSEELPPAIVRRLGQ
                     AGIEDPFCCAHWQRYRREGK"
     gene            complement(3239..>3471)
                     /locus_tag="ACZO9R_05385"
     CDS             complement(3239..>3471)
                     /locus_tag="ACZO9R_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912531.1"
                     /translation="ENTALHLSVLMNQGIYIDMIVKSVVENMIVGAILAIIVLLALGA
                     IIIAALSSDGYVATEFKNALTGFFSRMNGLTP"
BASE COUNT          875 a         1087 c          646 g          863 t
ORIGIN      
        1 aataccgcag gaccataccg atcatcccct gcccatccac ggcatggaag aataagagca
       61 atgcccctat tccgcccgca atctgcaaaa atccgttgcc ataaacctct acgatttctt
      121 tcatctgcac gctccttttc tctgacacgt ccggacacga aatcccccta tagcatgcca
      181 gccacctgca tccatatctc ctgaattgcc gtgcatccaa accagcccac cagcaatacc
      241 agcattgtca ttccaagttc ttccagactc cattgcattc tgctcctcct tttaccgtgc
      301 gattcctctc gtcacccgct gctgcgagac ccctaacagt ggtatccggt atctataata
      361 gagattgatc cccgcaatcc tgcggtcgct ttgtccaccg taggtgtagg actgcacttc
      421 cagttcgtac ccttctgttc ctgcctgcgc cttgcagcca tcaatcacgt acggattaaa
      481 gtcactgttc tcaatttctg caattattgc agctttaaat tccttcgccg ctgccacatc
      541 ggcactcacc ccgagcatac tgatgcacag cagcagatta aaaaccagca ccagcatcac
      601 actgaacact tctatgatat atttcactca cgcacctccc atgcctccaa gaacctgcat
      661 catcaacgcc attccgaccg taagatctgc cagcagtttt ccagtggctg ccagcaccgg
      721 atatgcaaaa atcaacttca tccggaatgc acgctcttca ctgcgcaaaa catcggcgcg
      781 ctcctgcatc agaaccacac gctcaatcag atgattcatc tgcacatcaa tatctcccgt
      841 tccgttttca gaaaaagcat gcagcatttt catacagctt aacatctcgg ggagatcaaa
      901 ctttttgcaa aagtccgtgt atgtctgtaa ttgatccgga cgctcgtcca ttctcgcgca
      961 aagctccgca agttcgctcc cgagcaccgc cggggcatgt tgtgcagatt ttgtaatcgc
     1021 catctgaacg ttattgttct gtaaaagtaa taccatctca aaaagccagc gcggcagcgc
     1081 ctgatacaat tccgccgtca cgtcccgctc ggcaatgcgg taactgattc tcggctgcat
     1141 tgcaaccagt aaaccagtca ccagcaggag tattcccgta atgcgccatc ccacagtcca
     1201 aaacacaaac gcagtcatag tcacaattcc tgcaggaatc aggcgccgat actgctgtgc
     1261 tttcttttca tatcccatta ccagttcata gctgtgtctg atatatgccg tgtcatacag
     1321 cggcttttcc tccagccagt cagccgtcag gcttctgaca ctcttcacat aaaatgcaag
     1381 cagcaacaaa agaaacacca tcgaagatat ctggatcaca ggaacggtaa acaccgatac
     1441 gtcaacctct gatgtaaaca tctcctgcat ctcatttaaa agatacaatg cagccgcgca
     1501 caagagcact gccaccacaa tggaaatcac attatctgta tgactcttcc gcttctccgc
     1561 ctgaagctgg tatccacgct ttttgaaccg ctcaatatcc tccagaacaa gcgtgaccga
     1621 tgcctccaca tccccgccat attcctccgt attcagtaga agttcatgta ccgtcgccag
     1681 cttatcacag gcataatacc gttcgatttt ctgtaaaccc tcacgcagaa cgccctgctc
     1741 tccgaccgga tgcccgtact ccatatgcgc aatcgcttcc tccacacaga gtcggatctg
     1801 tccttcccca aaaattccgc gcacctcctt caatgcactc acgatctttc cggtcttctg
     1861 aaacgcatag agcagctgtt ccatgtaggc acatgcatct ccaaaacgct tctgctcata
     1921 cattttctta tacatatcaa gtaccagtac cgggaatacg aaaagcactg tcaaaagcac
     1981 ccctgccaaa aacagcggtt ttagctgaaa caccattcca atcccaccga ttccaaccag
     2041 agcggctatc accgccataa gatgcgtcct ccacgaaaaa cgataaccgt acacatgcac
     2101 ttcgcgctgc agatttttcg gattcaacag ccgcagtttc tcccacagtc cttctctttt
     2161 catctatttt ccctctctcc tgtatctctg ccagtgtgcg cagcaaaacg gatcctctat
     2221 tcccgcctgt ccaaggcgtc tcacaatcgc aggcggaagc tcttccgaga gcagttcccc
     2281 atcctccagt atcatataaa tacgattctc atactgctcc ctgctataaa aacacagctg
     2341 atccatatat cttcggatct ttccgtccgg ctgtctgacg cagcggatca gaatccccac
     2401 attcacatag cggtagatcc ggttctccat acgctctgcg tcacgcgcat cgctcatcat
     2461 attctgaatg cggtccggaa gctttctgac atcatccagg tggatcgtcg taaagccgct
     2521 cacgccggta ctccactgct cgagcagcga cgtcacctcc accgaccttg cctccgagag
     2581 gacaagccac ttcggattct gccgcaggct cgccttgatg gcatccgtgt aggaaaaatc
     2641 ttctccgaca cgaagctcca ccgcatccgc ccccggattg atgtcccggt aatggatctc
     2701 gagcgaatcc tcaatcgtaa ttacccgctc ctcgcgcggt ataaactgca taaagaattt
     2761 cgcacactcc gtctttcccg caccgggttc tcctccgaac acaaaattca tgcccgccag
     2821 cacacagttc atcaaaagct gtaaaatccg ttccgggcaa taggattctt ccagcattct
     2881 ctcaatgttc atgcgcacca tgctcggcga cttccgaata cagatactgg tgccggatgc
     2941 ggccgccgcc tcatggataa tactgatacg cagttctctg gtatcagcct ccagcacctt
     3001 attggcatta ttgaactgac gattcacgca gttggaaata ttgtgcgtaa aagtgctgat
     3061 aaatgcatcg gacagatgct cttttacacg ataccttccg atctttaagt ccgtaatcca
     3121 caattcgctt ccgttgtagt ccacatcggt aacattcggg cgctgcagat atttccagaa
     3181 aaccccaaac tgcgcttccg ttggttcaaa tgtcatgatc tcccccatgt catcctccct
     3241 atggcgttaa gccattcatg cgggaaaaga atcccgtcag cgcattcttg aactccgtcg
     3301 ccacatatcc gtccgaggag agcgccgcaa taatgattgc gccgagtgca agcaatacaa
     3361 taattgcaag gatggctccc acgatcatgt tttctacgac tgatttcacg atcatgtcaa
     3421 tgtaaatacc ctgattcatg agaacgctca aatgtaacgc tgtattttct t
//
LOCUS       DBMDQJ010000206         3460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9941_length_3460_cov_1.750223, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000206 DBMDQJ010000000
VERSION     DBMDQJ010000206.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9941_length_3460_cov_1.750223"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(140..1735)
                     /locus_tag="ACZO9R_05390"
     CDS             complement(140..1735)
                     /locus_tag="ACZO9R_05390"
                     /inference="COORDINATES: protein motif:HMM:NF025622.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HIR0912532.1"
                     /translation="MKSVVMRDLRDYVRKYWGLLAASMAAMLICFGYLVFCGNIRIDT
                     EELINHPGSSLGWLAIGRWELAWLKHLLGLGTHQVIKSGILFFLFFWLGANLLTFGIY
                     ERSGKKKSRYWIFLLLYVTSNIWCYQIYFSLQQAEVALAMLLLIEAAFLMSGIGAGTV
                     TNLLRTFAAGVLLFIGLGSYQALAVYYIAVCLVFFLAELTAEREQGGYLRQILWMIVH
                     FGVVYLCYHWYMSTFLVSSDYMDSQMGWGRLPALACIKNVLRTLKNLLLGHGPRNFSF
                     YGCGVVLLLVLAGSVLACRKKQELPWEKKKIRYSLLGLAGLVLAPLLLTAYMGEMIVT
                     RSQFALPVAGAFLGMYVTERLAELWNGRDRGTGYWLLLVCRLCICLVIAVQTGYDLRL
                     AVTDEIRCREDEQKTQQLLERFAGADGGKLQQLPVVFVGYQEADLPEWCRRTEMYGWS
                     FYGWDYSMENPTGATHRICGFVQAYTGNVLREDATEEQKRHAVELAGQMKDFPEEGSV
                     LVTEDCVVVRLSEVKEQMPTDWW"
     gene            complement(1776..2648)
                     /gene="rfbD"
                     /locus_tag="ACZO9R_05395"
     CDS             complement(1776..2648)
                     /gene="rfbD"
                     /locus_tag="ACZO9R_05395"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HIR0912533.1"
                     /translation="MKKILVTGCNGQLGRAIRAEYAKEDVTFINTDVVEGDGVTALDI
                     ADVAAVLALVRAERPQVIINCAAHTNVDACETQWDAAYRINAIGPRNLAIAAAEVDAK
                     LIHVSTDYVFAGNGTRPYTEFDEPGPVSAYGKTKLEGERFVKAFARKYFIFRTAWLYG
                     DGKNFAKTMLRLAENHEEVSVVCDQLGSPTSAVELARAIHHYEPTENYGLFHATCEGD
                     TNWAAFAEAVFARAGKNTRVRHVTSEEYAAMNPASAKRPAYSILDNYMMRLTDGYRMA
                     DWESALDEYMQHLG"
     gene            complement(2671..2934)
                     /locus_tag="ACZO9R_05400"
     CDS             complement(2671..2934)
                     /locus_tag="ACZO9R_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR0912534.1"
                     /translation="MVSQKVTVKNPTGLHLRPAGILCKEAMQFKSHITFRYRENTANA
                     KSVLSVLGACIKSGDEIELVCEGEDEQEALAALVNAIENGLGE"
     gene            complement(2981..>3460)
                     /locus_tag="ACZO9R_05405"
     CDS             complement(2981..>3460)
                     /locus_tag="ACZO9R_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR0912535.1"
                     /translation="FTLMGEKEGIHVEKTKVGDRYVLENMREHGYNIGGEQSGHVIFL
                     DDNTTGDGLLSALHLLEVMVDTGKPLSELAAIMEVLPQALVNAKVPNHKKEHFMEYPE
                     IADAIAELEKKFNGEGRVLIRPSGTEPLVRVMIEGKDQKVIDEEAKRLADLITRIML"
BASE COUNT          780 a         1120 c          721 g          839 t
ORIGIN      
        1 agttctcatc tcatcctcca tttatttcca atacttgcag tctctattat ccatggccgt
       61 ttcactgcca taagtctctt cattttatca tagattccgt tccagtacaa gcttgtatac
      121 acagaccgcc gtttcacctc taccaccagt ctgtcggcat ctgctcctta acctccgaca
      181 gccgtaccac aacacaatcc tccgtcacaa gaaccgatcc ctcttccggg aaatccttca
      241 tctgtcccgc cagttccacc gcgtgtctct tctgctcttc cgtcgcatcc tcccgcagca
      301 cgttcccggt ataagcctgt acaaaaccgc aaatacggtg tgtcgctccg gtcgggttct
      361 ccatactgta atcccaaccg tagaacgacc agccatacat ctcggtccgc ctgcaccact
      421 ctgggagatc tgcctcctga tagcccacaa acaccaccgg aagctgctgc agtttccctc
      481 cgtctgcccc ggcgaagcgc tctaacagct gctgggtttt ctgctcatcc tccctgcagc
      541 gtatctcatc ggtcacggca aggcgcagat catagccggt ctggaccgca atcacaaggc
      601 agatgcacaa tctgcagacg agcagcagcc agtaacccgt accgcggtct ctgccgttcc
      661 agagttccgc aagccgctcc gtcacataca ttcccaggaa cgctcccgcc accggaagcg
      721 caaactgtga tctcgtcacg atcatctctc ccatgtatgc cgtcagaagc agcggcgcaa
      781 gcaccagtcc cgccagtcca agcaatgaat accggatttt cttcttctcc cacggaagtt
      841 cctgtttttt ccggcaggca agcacacttc ctgccagcac aagcagaagc actacgccgc
      901 atccgtagaa cgaaaaattt ctggggccat ggccgagcag cagatttttc agcgtgcgca
      961 gcacattctt gatacatgcg agcgcaggca gtcttcccca gcccatctgg ctgtccatat
     1021 aatccgaaga caccagaaac gtgctcatat accagtgata gcacagatac accacaccaa
     1081 aatgtacgat catccagaga atctgccgga gatatccgcc ctgctcccgc tccgcagtca
     1141 gctccgccag gaagaaaacg aggcacactg cgatgtagta aaccgccagc gcctgatacg
     1201 atcccagacc gataaataac agaacgcccg ccgcaaaagt ccggagcaga ttcgttacag
     1261 ttccggcgcc gatcccgctc atcaggaatg ccgcctcaat caacagcagc attgcaagcg
     1321 ccacttccgc ctgctgcagg gaaaaataaa tctgatagca ccagatgttg gacgtcacat
     1381 agaggagcag gaaaatccag taccggcttt tctttttccc ggagcgctca taaatgccga
     1441 acgtcagaag attggctccc agccagaaaa acaggaagaa caggatcccg ctttttataa
     1501 cctgatgtgt tccaagcccc agaaggtgct tgagccacgc cagctcccac cgtccgatcg
     1561 caagccatcc cagactgctt cccggatggt tgattaactc ttccgtatca atgcggatgt
     1621 tgccgcaaaa aacaagataa ccgaaacaga tcagcattgc cgccattgac gcagcgagca
     1681 gcccccagta tttcctcaca tagtcacgca aatcccgcat tacaacactc ttcatctgtt
     1741 ccggttatcc tttcactttt ctgtcggtat ctctctcagc ccaaatgctg catatattca
     1801 tccagcgcgc tctcccagtc agccatacga tagccgtccg tcaggcgcat catgtaatta
     1861 tccagaatgg aatacgccgg tctcttcgcc gaagcaggat tcatcgccgc atactcctca
     1921 cttgtcacat gtctgacgcg ggtattcttc ccggcacgcg caaacactgc ctccgcaaat
     1981 gccgcccagt tggtatcccc ctcgcaggtt gcatggaaca gtccgtaatt ctccgttggt
     2041 tcatagtgat gaatcgcacg tgcaagttcc actgcactgg tcggcgaacc gagctgatcg
     2101 cataccacgc tcacttcctc atggttctct gcgagtctca acatcgtttt ggcaaaattt
     2161 ttgccgtccc cgtacagcca tgccgtgcgg aagataaaat actttctcgc gaacgccttc
     2221 acgaagcgtt ctccctcaag ctttgttttg ccgtatgcac tgaccggtcc cggctcatcg
     2281 aattccgtat acggtctcgt tccgtttccg gcaaagacat aatccgtgga cacatgtatc
     2341 agctttgcgt caacttccgc agcagcgatg gccaggttgc gtggaccgat cgcgttaatg
     2401 cgatacgccg catcccactg tgtctcacag gcatccacat tcgtgtgcgc cgcacagttg
     2461 atgataacct gcggacgctc ggcgcggaca agcgccagca cggcagccac atcggcgatg
     2521 tcaagtgctg tgacaccatc gccctccacc acatctgtgt tgataaacgt tacgtcttcc
     2581 tttgcatact ctgcgcggat tgcgcgcccc agctgaccgt tacagccggt aaccagtatc
     2641 tttttcatcc ggttttcctc tcttactctt ctattctcca agtccgttct caatggcatt
     2701 cacaagcgcc gcaagcgctt cctgttcgtc ttccccctcg cataccagct caatctcatc
     2761 gcccgatttg atgcatgcac cgagcacgct caacacgctc ttggcatttg cagtattctc
     2821 acggtatcgg aatgtgatgt gcgatttgaa ctgcatcgcc tccttgcaca ggattcctgc
     2881 cggtctaaga tgcagccccg ttggattctt gactgtgact ttctgactta ccatgctctc
     2941 ttccctccaa tgaataattt tcctaacaaa gtctctgtct ctacagcata atgcgcgtaa
     3001 tcagatccgc aagtctcttc gcttcctcgt caatcacctt ctgatctttc ccctcaatca
     3061 taacgcgcac cagaggttcg gtaccggacg gccggatcag cacgcgtccc tcaccgttaa
     3121 actttttctc cagttccgcg attgcatctg caatctccgg atactccata aaatgctctt
     3181 ttttgtgatt cggcaccttg gcattgacaa gcgcctgcgg cagaacctcc atgatcgctg
     3241 ccagctctga caacggcttt ccggtgtcca ccatcacttc cagaagatgc agggcgctca
     3301 aaagcccgtc tccggtcgta ttgtcatcca ggaaaataac gtgtccggac tgctcacctc
     3361 cgatattgta tccgtgttcc cgcatgttct ccagaacata ccggtctccg acttttgttt
     3421 tctctacgtg gattccctct ttttctccca tcagagtaaa
//
LOCUS       DBMDQJ010000207         3452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9969_length_3452_cov_1.403163, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000207 DBMDQJ010000000
VERSION     DBMDQJ010000207.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9969_length_3452_cov_1.403163"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(147..1055)
                     /gene="era"
                     /locus_tag="ACZO9R_05410"
     CDS             complement(147..1055)
                     /gene="era"
                     /locus_tag="ACZO9R_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR0912536.1"
                     /translation="MKDNFKSGFVTIIGRPNVGKSTLMNHLIGQKIAITSNKPQTTRN
                     RIQTVYTDMERGQIVFLDTPGIHQAKNKLGEYMVNVAEHTLSEVDVILWLVEPSTFIG
                     AGEQHIIKQLKKTKTPVILIINKVDTVEREKILEYLDAYRKVFDFAEIVPASALREQN
                     LDTVVDVIFKYLPYGPMFYDEETVTDQPERAIVAEIIREKALHALDDEIPHGIAVYID
                     RMKERRGQNIIDIDATIVCERDSHKGIIIGKGGSMLKKIGSNARYEMERLLDTQVNLK
                     LWVKVRKDWRDSELMMKNFGYNKDDI"
     gene            complement(1094..>3452)
                     /locus_tag="ACZO9R_05415"
     CDS             complement(1094..>3452)
                     /locus_tag="ACZO9R_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR0912537.1"
                     /translation="ANESGGDPEHIPELTYEQYLNFHRKYYHPCNSYIYLYGDMDVAE
                     KLRWMDEEYLGKYEQIELDSAIRMQKPFAAPVEIVKPYPVASGEPLTDNSYLSYNVVV
                     GTVLDKKLYQAFDVLDYALLSAPGAPLKQALIDAGIGMDIGGGYDNSTLQPVFSVIAK
                     NANPEQKEQFLQVIRETLQAKVADGIDQNALLAGINSSEFRFREADFGQFPKGLLYGI
                     QCLDSWLFDDMQPFMHLEALDTYQFLKDQIGTGYYEELVKTYLLDNPHASVVITVPEQ
                     GLGAKRDAALEKKLAEYKAGLSREERKKLIADTKHLKQYQEEPSPKEDLEKIPRLTRA
                     DLKKEAAPLLNREIETEGVSIVAHEMDTNGIDYLTYLFDVCDILPEDLPYLGILKAVL
                     GYVDTDNHSYAALANEINMYTGGIGSSIGIYPNVKKQGEIGLYYEVRTKVLASRLPDA
                     MRLIKEILLTSHLSDEKRLYEILAQLKSRLQAGLSASGHSVAYTRALSYFSAAAYCQD
                     ATAGIACYRVIADYEEHFEEKKQQLMEKLFSLVKTIFIAERMLVSATCEESEIPAVTQ
                     ASAALKNVLYVPDQPGKNRSMPSYPLAKKNEGFMDASQVQYVARAGNYAAHGFSYHGA
                     LRILKVILGYDYLWINVRVKGGAYGCMNGYMRNGDTYFVSYRDPNLSATNEIYDGIPA
                     YLEKFDADEREMTGYIIGTISDMDTPMNPSAKGARSMTAYLQELDYETLSKERSQIIG
                     AQPADIRALKPLIASVLSDGSLCVIGNEDVLTSEKQMFETLESLT"
BASE COUNT          749 a         1044 c          753 g          906 t
ORIGIN      
        1 cgcaggtaat cagtcactct tcctgatact aaaaagtctc tccaggcttc ctccatcggc
       61 atatcctccg tttctttgac gcttcccgcc tctaagatta ggttctccga tggctccgcc
      121 attataccag caaaaaattg gcagtatcag atatcgtctt tattgtaccc gaagttcttc
      181 atcatcagtt cgctgtcgcg ccagtccttg cgcaccttca cccacagttt taagttcacc
      241 tgcgtatcca aaagccgctc catctcgtag cgggcgttcg atccgatctt ttttaacatc
      301 gaaccgcctt ttccgatgat gatgcccttg tgggaatcgc gctcacagac gatcgttgca
      361 tcgatgtcga tgatgttctg ccctctccgc tccttcatcc ggtcaatgta gaccgcgatg
      421 ccgtgcggga tctcatcatc cagagcatga agcgccttct cacggataat ctccgcgaca
      481 atggcgcgtt ccggctggtc tgtaaccgtc tcctcgtcat agaacatcgg tccgtacggc
      541 aggtacttga aaatcacgtc cacaaccgta tccagattct gttcccggag cgccgatgcc
      601 ggaacgatct ctgcaaaatc aaacaccttg cggtaagcat cgagatactc caggatcttt
      661 tcccgctcca cggtatccac cttgttgata attaagatga ccggcgtttt cgtcttcttt
      721 agctgcttta tgatatgctg ctcgcctgcc ccgataaacg tggacggctc caccagccag
      781 agaatgacat ccacctccga aagcgtgtgc tccgccacat tcaccatata ctctccaagc
      841 ttattcttcg cctgatgaat gcccggcgta tccaaaaaca caatctgtcc gcgctccata
      901 tccgtataca cggtctggat gcggtttctg gtcgtctgcg gcttgttcga cgtgatagca
      961 atcttctgac cgatcagatg attcatcagc gtcgattttc ccacattcgg tcttcctata
     1021 atcgtcacaa atccggattt aaaattatct ttcatcgttc tcctcgtttc tgtactgttt
     1081 tccccgcccc gcatcaggtc aggctctcaa gtgtctcaaa catctgtttt tccgatgtca
     1141 gcacatcttc attgccgatc acacacaggc tgccgtctga gagcaccgac gcaatcaaag
     1201 gcttcaatgc gcggatgtcc gccggctgtg ccccgataat ctggctccgc tcctttgaaa
     1261 gcgtctcgta gtccagctcc tgcagatagg ctgtcatcga acgcgcaccc ttcgcgctcg
     1321 ggttcatcgg cgtgtccata tcgctgatcg ttccgatgat atatcctgtc atctcgcgct
     1381 catcggcatc aaacttctca aggtacgccg gaatgccgtc ataaatctca ttcgttgcag
     1441 agagattcgg atcccggtag gacacaaaat acgtatctcc gttcctcata tatccgttca
     1501 tgcagccata agcgccgccc ttgacgcgga cattgatcca caggtaatcg tatcctaaaa
     1561 ttacttttaa gatgcggagc gccccgtgat aggaaaagcc gtgtgcggca taatttccgg
     1621 cacgcgccac atactgcacc tgggatgcat ccataaatcc ctcgttcttt tttgccagcg
     1681 gataggacgg catagagcga ttcttacccg gctgatccgg cacatacaat acatttttca
     1741 atgcagccga cgcctgcgtc accgccggga tctccgactc ctcgcaggtt gcactcacca
     1801 gcatacgctc cgctataaaa atggttttca caagggagaa cagcttctcc atcagctgct
     1861 gctttttctc ctcaaaatgc tcctcgtaat ccgcgatcac acggtagcag gcaatgcccg
     1921 ccgtcgcgtc ctggcagtag gcagccgccg aaaaatacga cagcgctctc gtatatgcca
     1981 cggaatgtcc ggaagcgctc agtcccgcct gtaatctcga ctttaactgt gccagtatct
     2041 catagagcct cttctcatcc gacagatgcg aggtcagaag gatctcctta atcaagcgca
     2101 ttgcatccgg cagtctcgat gcgagcactt tcgtgcgcac ctcatagtaa agtccgattt
     2161 cgccctgctt tttgacgttc gggtagatgc cgatgctgct gccgattcct ccggtgtaca
     2221 tattgatctc attggcaagc gccgcgtagg aatgattgtc cgtatccaca tatcccagca
     2281 ctgcctttaa gataccgaga taaggcagat cttccggcag aatatcgcac acatcaaaca
     2341 gataggtcag gtaatcgata ccattcgtgt ccatctcatg cgcaacgatg gaaacgccct
     2401 ccgtctcaat ctcacggtta agaagcggcg ccgcctcttt tttcagatcc gctctcgtaa
     2461 ggcgtggaat cttctctaag tcttccttcg gcgacggctc ttcctgatac tgctttaagt
     2521 gcttcgtgtc tgcgatcagc ttctttcgct cttccctgga caatcccgct ttgtattcgg
     2581 ccagcttctt ctccagcgcg gcatctctct ttgcgcccag cccctgctcc ggcaccgtaa
     2641 tcactactga cgcatgcgga ttgtccagaa gatatgtctt taccagttcc tcataatatc
     2701 cggttccgat ctgatccttc aaaaactgat aggtgtccag cgcttccaga tgcataaacg
     2761 gctgcatatc atcgaacagc cagctgtcca gacactggat tccgtagagc agtcccttcg
     2821 gaaactgtcc gaaatccgcc tcccggaaac ggaattccga gctgttgatt ccggctaaca
     2881 atgcgttttg atcaattccg tctgccacct ttgcctgcag cgtctcacgg atcacctgca
     2941 aaaactgctc cttctgctcc ggattcgcat tttttgctat cacagaaaat accggctgca
     3001 gcgtgctgtt atcatagccg ccgccgatgt ccataccgat tcccgcatcg atcagcgcct
     3061 gcttaagcgg cgctcccggc gcagaaagca gtgcgtagtc caaaacgtca aacgcctggt
     3121 ataacttttt gtccagaacg gttccgacca ccacattgta agacaggtag ctgttgtccg
     3181 taagcggctc ccccgatgcc accggataag gctttacgat ctccaccggc gcggcgaacg
     3241 gcttctgcat ccggattgcg gaatccagct cgatctgctc gtattttccg aggtattcct
     3301 catccatcca gcgcagcttt tctgcaacgt ccatatcccc gtacagatag atataggaat
     3361 tgcacggatg ataatacttt ctgtggaagt tcagatactg ctcataggta agctccggaa
     3421 tatgctccgg gtcgccgccg gattcgtttg cg
//
LOCUS       DBMDQJ010000208         3447 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_9995_length_3447_cov_1.562164, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000208 DBMDQJ010000000
VERSION     DBMDQJ010000208.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9995_length_3447_cov_1.562164"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="ACZO9R_05420"
     CDS             <1..1179
                     /locus_tag="ACZO9R_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HIR0912538.1"
                     /translation="LYRTNAQSRVLEEKLVTANIPYRIVGAVNFYQRREIKDLLAYLR
                     TVENGLDDISVKRIINVPKRGIGATTLNRVSDYAEAYDISFYESLKRAEEIPSLGKSA
                     AKIKPFVTFIQTMRSKMPYIGVADLLREVIEETGYVKELEAEGTDEAEARIENIDELL
                     SKVVAYEEGEEQPTLSGFLEEVALVADIDSVGEENDYVVLMTLHSAKGLEFPKVFLAG
                     MEDGLFPSYMSITSDNASSELEEERRLAYVGITRAMKELVITSARQRMVRGETQYNKV
                     SRFVKEIPPELLNGEIKKPAYLENRERHTAEPVKKKRPTMRDQLSGQSMQARAFSSVH
                     TEGGSLSYGVGDRVSHMKFGEGTVKAIVPGGRDFEVTVDFDAAGTKKMFAAFAKLKKV
                     "
     gene            1288..1581
                     /locus_tag="ACZO9R_05425"
     CDS             1288..1581
                     /locus_tag="ACZO9R_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912539.1"
                     /translation="MNKMEDLINMTKLNELLNKKEEVEEKKTSKVVWIFAIIGIVAAV
                     AAAVYGIYRFFTPDYLEDFEDDFEDDFDDDFFEDDDDEEAPAKSEDKEETTED"
     gene            1858..3282
                     /gene="rlmD"
                     /locus_tag="ACZO9R_05430"
     CDS             1858..3282
                     /gene="rlmD"
                     /locus_tag="ACZO9R_05430"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HIR0912540.1"
                     /translation="MKKGQIIEGMVECVEFPNKGIVRTEDGGRVIVKNAVPGQKVSAS
                     INKVRKGKCEGRLLEVLERSPKEQPEAACIHAGECGGCTYQTLPYEEQLAMKASQVKK
                     LIDDVIVPENTDYEFLGIKASPRQQEYRNKMEFSFGDAYKDGPLALGMHKRGSFYDIV
                     DVPECRIVDEDFHTVLTVTLSYFKERNLPYYHKLRHTGYLRHLLVRKAVKTGEILVDL
                     VTTTQIVEGEEEVLLAGWKEALCAAAYRGTLTGVLHTRNDSVADTVTNEGTDVLFGQD
                     HFYEELLGLRFQITPFSFFQTNSLGAEVLYETAREFIGDALPSGADADVAEHGKVVFD
                     LYSGTGTIAQMLAPVAKKVIGVEIIPEAVEAAKENARLNSLTNCEFIAGDVLKVIDEI
                     EEKPDYIVLAPPRDGIHPKALEKIIRYGVPQMVYISCKPTSLARDLEVLQARGYEVKK
                     VCCVDMFPATVHVESCVLLCRTDT"
BASE COUNT          942 a          677 c         1070 g          758 t
ORIGIN      
        1 ttgtatcgta cgaatgcaca gtctcgtgta ttggaggaaa aactggtgac ggccaatatc
       61 ccatatcgta ttgtcggtgc ggtaaacttc taccaacgcc gtgagatcaa ggatctgttg
      121 gcatatttgc gcacagtgga aaacggttta gatgatattt ccgtaaaacg aatcatcaat
      181 gtaccgaagc gcggaattgg tgcgacgacg ctaaatcgtg tatccgatta tgcggaggcg
      241 tacgacatca gtttttacga gtccttaaag cgtgcggagg agataccgtc ccttggcaag
      301 agcgcagcga aaatcaagcc gtttgtgacg tttattcaga cgatgcgcag caagatgccg
      361 tatatcggtg tggcggatct gctcagagag gtgatcgagg agacgggcta cgtgaaggag
      421 cttgaagcgg aaggcaccga cgaggcggag gcgcggatcg agaatatcga tgagttgtta
      481 agtaaggtgg tcgcctacga ggagggcgaa gaacagccga cattaagcgg tttcttagag
      541 gaggttgcac tcgtggcgga tattgacagc gtcggcgagg agaacgatta tgtggttctc
      601 atgacactgc acagcgccaa aggtctggag ttcccgaagg tctttctggc gggaatggag
      661 gacggtttgt tcccgagcta tatgtcgatc acgtcagaca atgccagcag tgagctggag
      721 gaagaacgca ggctggccta tgtcggcatt acacgagcga tgaaggagct tgtcatcacg
      781 tcggcgagac agcgtatggt gcgcggcgag acacagtaca acaaggtctc acgctttgtg
      841 aaggagattc cgccggagct tttaaacggc gagatcaaaa agccggcgta tctggagaac
      901 agggagcggc acacggcgga gccggtgaaa aagaaacggc cgacgatgcg ggatcagctg
      961 tccgggcaga gtatgcaggc gagagcgttt tcatcggtac atacggaagg cggaagctta
     1021 agctacggtg tgggcgaccg tgtctcccac atgaagttcg gggagggaac ggtaaaagcc
     1081 atcgtgcccg gcggcaggga ttttgaggtg acagtcgatt ttgacgcggc gggaaccaag
     1141 aaaatgtttg cggcgttcgc aaaattaaaa aaagtataaa atatgttaat ttataaattt
     1201 tttataggcg aacagggaaa gatatgctat aatgtgcgta ataagaagac atgcaataaa
     1261 tgaagataaa tcaaggaggg gaatctgatg aataagatgg aagatctgat caacatgaca
     1321 aagttaaacg aacttctgaa taagaaggaa gaagtggagg agaagaagac ctcaaaggta
     1381 gtatggattt ttgcaattat cggtattgta gcggctgtag ctgcagcagt atacggtatc
     1441 taccgtttct ttacgccgga ttatcttgag gatttcgagg acgatttcga ggatgatttt
     1501 gatgatgatt tcttcgagga cgatgatgat gaggaagcgc cggcaaagtc tgaggacaaa
     1561 gaggaaacaa ccgaagatta agacaaaggg catggggctg cagagaggtg cagccctatt
     1621 tttatgtaac aggaaacgct ctgtggggat gcgcacctcg tggtgaggta aataatgtct
     1681 gcataccaca tcaggcagcc tgccaagaca ggcgcaagtg tgcggcaggc gcacacttta
     1741 ttctgtagca ggaaacggta tcgtaagtgg ctgagtcagg cgagtgaatc ctgcatgcag
     1801 gattttttat gtttggcaat gtgcagaaga aactggacgg ataatggagg atagaacgtg
     1861 aaaaagggac agattatcga ggggatggta gaatgcgtag agttcccaaa caagggaatt
     1921 gtgagaacgg aagacggggg acgcgtgatt gtaaaaaatg cagtcccggg acagaaagtg
     1981 tccgcatcaa tcaataaagt gcgcaaagga aaatgcgagg gaagactgct tgaggtgctg
     2041 gagcgttcgc cgaaggagca gccggaggct gcctgtatcc atgccggaga gtgtggaggc
     2101 tgcacatatc agacgctgcc ttacgaggag cagcttgcca tgaaggcgtc acaggtaaaa
     2161 aaactgatcg acgatgtcat cgtaccggaa aatacagact acgaattcct tggaatcaag
     2221 gcgagcccga gacagcagga gtaccggaat aagatggagt tttcgttcgg cgatgcgtac
     2281 aaggacggac cgctggcact tggcatgcac aagcgcggca gcttctacga tatcgtggat
     2341 gtcccggaat gccggattgt ggacgaggat tttcatacgg tgctcacagt gacgctgtct
     2401 tattttaaag agcggaatct gccgtattat cacaaactgc gccacaccgg atatctgcgt
     2461 catcttctgg tacgcaaggc ggtgaagacc ggggagatcc tggtggatct ggtgacgaca
     2521 acgcagatag tggaagggga ggaagaggtg cttcttgccg gatggaagga ggcgctgtgt
     2581 gctgccgctt atcggggaac actcaccggt gtgcttcaca cccgcaacga cagtgtggcg
     2641 gacaccgtca caaacgaggg cacggatgtg cttttcgggc aggatcattt ctatgaagaa
     2701 ttgttaggac ttcgtttcca gatcacgccg ttttcctttt tccagacgaa ctctctgggc
     2761 gcggaggtgc tctatgagac agcacgggag ttcatcgggg atgccctgcc gagcggagcg
     2821 gatgcagatg tcgcggagca tggaaaagtc gtgttcgatc tttattccgg aaccggtacg
     2881 atcgcgcaga tgctcgcccc ggttgcaaaa aaggtgatcg gcgtggagat tattccggag
     2941 gcggtcgaag cagcgaagga gaatgcaagg ttaaacagtc ttacaaactg cgagtttatc
     3001 gcgggagatg tgctcaaggt gattgatgag attgaggaga agccggatta tatcgtgctc
     3061 gctccgccgc gtgacggcat ccacccgaaa gctctggaga agatcatccg ctacggtgtg
     3121 ccgcagatgg tgtatatctc ctgcaaaccg accagcctgg cgagagacct tgaggtgctg
     3181 caggcgaggg ggtatgaggt gaagaaggta tgctgcgtgg atatgttccc ggcgactgtg
     3241 catgtagaga gttgtgtgtt gctatgtcgc accgatacct agaaatcctt gattttacgc
     3301 agtttttcga ccatttcgta tttacgccaa atgcggaaaa tgtgttagaa aaggagttaa
     3361 aaagagaaat tcctgtgagt gtcggaattg aactggtagt ggtggacatg aggaaataaa
     3421 tgggatatta taataatata gtattat
//
LOCUS       DBMDQJ010000209         3441 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10012_length_3441_cov_1.455988, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000209 DBMDQJ010000000
VERSION     DBMDQJ010000209.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3441)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3441)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3441
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10012_length_3441_cov_1.455988"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="ACZO9R_05435"
     CDS             complement(<1..605)
                     /locus_tag="ACZO9R_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR0912541.1"
                     /translation="MRLLIVRHGDPDYEHDTLTEKGWREAELLSERLSKLPVKEFYVS
                     PLGRAKDTASLTLQKMNREAKECLWLREFDTKIDRPDCPDQKSIAWDWLPEDWTREEE
                     FFRRDLWATQQTMREGLAPGAHEGNGIRVQKKPDVGVGAEYGWVVSNFDALLAEHGYV
                     REGDIYRVAKANRDTLVFFCHFGLECVLLSHLMNVSPMILW"
     gene            complement(602..1883)
                     /locus_tag="ACZO9R_05440"
                     /pseudo
     CDS             complement(602..1883)
                     /locus_tag="ACZO9R_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079974.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     assembly_gap    1943..1952
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2026..2541)
                     /locus_tag="ACZO9R_05445"
     CDS             complement(2026..2541)
                     /locus_tag="ACZO9R_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HIR0912542.1"
                     /translation="MRNSKVQFIVHAAMIAAIYVVLTYFISAFNLASGAIQVRISEAL
                     TILPYFTPAAIPGLFVGCLLANLLTGAAIYDVIFGSLATLLGAVGTYLLRKHKFLCTL
                     APVVSNIIIIPLVLRYGYGLTMEYGGRDWSIPFYMLTVGAGEIICCCVLGTILLNALA
                     KVRNAIFKKED"
     gene            complement(2719..>3441)
                     /gene="dapF"
                     /locus_tag="ACZO9R_05450"
     CDS             complement(2719..>3441)
                     /gene="dapF"
                     /locus_tag="ACZO9R_05450"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HIR0912543.1"
                     /translation="GVGADGILYGPFFDGDKMRVRIFNPDGSEAEKSGNGIRIFSKYL
                     KDMGYVTEESYVLHTKAGETRVTFLNEQGDLMRVDMGYPEFVAKEIPVVGFEGEIVNE
                     AIFFGDNFYNATCVSLGNPNCVLMFEEVSQKKAMQLGPYVENSRYFPNRINMQLCQVL
                     DRENIQIEIYERGAGYTYASGTGACAAAAASHKMGFVGDRVTVHMHGGELLVEFARDG
                     RIDMTGPVVYIGSITLAEQFFA"
BASE COUNT          793 a         1000 c          787 g          851 t
ORIGIN      
        1 tgccacagaa tcatcggtga cacattcatc agatgcgaca gcagcacaca ctccagaccg
       61 aagtggcaga agaacacaag cgtatcgcgg ttcgcctttg cgacgcggta gatgtcgccc
      121 tcgcgcacat agccgtgctc cgccagcagg gcatcaaaat ttgataccac ccagccatac
      181 tctgcaccta cgccaacgtc cggtttcttt tgcacacgga tgccgtttcc ctcatgcgct
      241 ccgggagcca gtccctcacg catggtctgc tgcgtcgccc acaggtcgcg ccggaagaac
      301 tcctcttctc tcgtccagtc ctccggcagc cagtcccagg cgatcgactt ttgatccggg
      361 caatccggtc ggtcgatctt cgtatcgaac tccctgagcc acagacattc ctttgcctcg
      421 cggttcatct tctgtaacgt cagggacgcc gtatccttcg cccgcccgag cggcgacaca
      481 tagaactcct taacaggaag ctttgacagg cgttctgaca aaagctctgc ctcgcgccag
      541 cccttttctg tcagggtatc atgctcataa tccggatccc cgtgccgtac aatcaataat
      601 ctcatactgc cgcaaatgcc tccgcaagat cttcaataat atcgtccaca ttctctgttc
      661 caatcgacag acggatggtg ttcggcttga tgccctgctc taacagttcc tccgtcgtaa
      721 gctgggaatg tgtcgtggat gccggatgaa tcacgagcga cttcacatcc gccacattgg
      781 caagcagaga gaacagctcc agatgatcga tgaagtcttt tgcggtctgc tcgtctccct
      841 tgatctcgaa cgtaaagatc gaaccgccgc cgttcgggaa atattttttg tagagtgcct
      901 gctgcaccgg atcctccgat accgacggat gatgcacctt ctccaccttc gggttgttgt
      961 tcagatattc tacgaccttt aatgcattct ccacatggcg ctccacacgc agcgacaatg
     1021 tctcaagtcc ctgtaaaaac aggaaagagt ggaacggaga gatggttgca ccggtgtcgc
     1081 gcagtaaaat ggcgcggatc tttgtgacga acgctgccgg tcctgccgcc tttgtaaaac
     1141 tgatgccgtg gtagctcgga ttcggctccg tcaaggacgg gaacttgccg cttgcctccc
     1201 agtcaaactt tccgccgtcc acgatcacgc cgccgatggc tgttccgtgt ccgccgataa
     1261 acttggttgc cgagtgaacg acaatatctg ctccgtactc aagcgggcgg accagatacg
     1321 gcgttgcaaa agtgttgtcc acaaccagcg gaatctgatg cgcatgcgcg atcgctgcga
     1381 tcttctcaat atccacaacg tccgagttcg ggttgccgag cgtctcaatg tggatcgctc
     1441 tgctccacct cgtcgtaatt gaatggatcg acgaaagttg tcgtgatacc atactccggg
     1501 aatgtatgtg ccaatagatt gtaggtgccg ccgtagatgt tgtttgcagc cacgatgtgc
     1561 tcgccagcat gcgccagatt ctggagtgta taggcgatcg ccgccgcacc ggaagccacg
     1621 gcaagcgctg ccacaccgcc ctccaaagct gcgattctct gctcgaagac atcctcggtc
     1681 gggtttgtta atctgccata gatatttccc gcatcggtca gtccgaaacg cgccgccgca
     1741 tgctcactgt tgttaaatac ataagaagat gtctggtaga tcggcactgc tctcgctccg
     1801 gtcaccggat ctgcctcctc ctgtcctacg tgaagctgta atgtctcaaa ttttaacttt
     1861 ctgtccttta atgcttttcc catgttatgt ttctcctttt attttcattt tggaagttct
     1921 gtctgttgat aatctgatga tannnnnnnn nnagaaaacc aacctgtcta atatgaaaaa
     1981 aaaagcacca gttataggtt attcctatag ctggtgtttt ctaatctaat cctctttttt
     2041 aaagattgca ttgcgcactt ttgcgagtgc attcaaaaga atcgttccaa gcacacagca
     2101 gcagatgatc tctcccgctc cgacggtcag catgtaaaac ggaatcgacc agtctcttcc
     2161 gccatattcc atggtaagtc catatccgta gcggagtacc agcggaatga tgatgatatt
     2221 tgataccacc ggcgcgagcg tgcataaaaa cttgtgcttg cggagcagat atgttccgac
     2281 tgcgccgagc agcgtcgcca gactgccgaa gatcacatca tagatggctg ctcccgtcag
     2341 aaggttggca agcagacatc ccacaaacag tcccggaatt gccgccggtg taaagtacgg
     2401 cagaatggtc agcgcctcgg aaatccggac ctgaatggct cccgatgcca ggttgaacgc
     2461 actgataaaa taggtcagca cgacatagat tgctgcaatc atcgcagcgt gcacaatgaa
     2521 ttgtacctta gagtttctca tattctgttc tcctttagtt ttgttttacg agtggaaggt
     2581 ctcgaacact cgattcattt aacgccggaa atcggcggca agcccgctta gaccttgttt
     2641 ttatgggcta cgcaacgtct gacattttat cacagtctgc caaaaaaggc aacggcaaaa
     2701 tttctcccgt aaaatttttc acgcaaaaaa ctgctccgcg agcgtaatgc tcccaatata
     2761 aaccaccggt cctgtcatgt caattctgcc gtcccgggca aattccacca gaagctcccc
     2821 gccgtgcata tgcaccgtca cacggtcgcc gacaaatccc atcttgtgcg atgcggcagc
     2881 cgctgcacag gctcctgttc ccgacgcgta tgtataaccc gcgccgcgct catagatctc
     2941 gatctgaata ttttcccggt cgagcacctg acagagctgc atgttgatac ggttcggaaa
     3001 atatctggaa ttttccacat acgggccaag ctgcatcgct tttttctggc tgacctcctc
     3061 gaacatcagc acacaatttg gatttcccag ggagacgcag gtcgcgttat agaaattatc
     3121 gccaaaaaag atcgcctcgt tgacaatctc cccctcaaac ccgaccaccg ggatttcctt
     3181 tgccacaaac tccgggtatc ccatgtccac gcgcatgaga tcgccctgct cgtttaaaaa
     3241 cgtcaccctg gtctcacccg cttttgtgtg cagcacatag ctctcctctg tcacataccc
     3301 catatctttt aaatatttgg aaaaaatgcg gataccgttt ccgctcttct ccgcctcact
     3361 cccgtccggg ttgaaaatgc gcacccgcat tttgtcccca tcaaaaaaag ggccatagag
     3421 aatgccgtct gctccgactc c
//
LOCUS       DBMDQJ010000210         3424 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10060_length_3424_cov_1.516702, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000210 DBMDQJ010000000
VERSION     DBMDQJ010000210.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10060_length_3424_cov_1.516702"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="ACZO9R_05455"
     CDS             <1..1149
                     /locus_tag="ACZO9R_05455"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019318 - hexose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HIR0912544.1"
                     /translation="WQLDDVIGFDNDGGLTGGIQTTGNYPGRARTPQQLMEDMEEAMK
                     LMPGTAKLNIHASYAIFEPGEFADRDALEPKHFAKWVEFARKHHMGIDFNPTFFSHEK
                     VKDGLTLSSPDEETRNFWINHGKACIRISEYFAKETGVPCVMNIWTGDGFKDVPADRM
                     GPRLRYKDSIEQILSEPYDRKLVKPCVESKVFGIGVESYTVGSAEFTLSFAAQHEGCM
                     PLMDNGHYHPTEYVSDKIPAMLCFYPEFALHITRGVRWDSDHVLLLDDETKEMAKEIV
                     RNDALDRVYMALDYFDASINRVLALATGFRSWQKALLTALCTPNEMLKELQDTNQLGK
                     LMVMHEAVKMLPIGEIWEEYCRREGVCDDLHFYDEIERYEREVLTKRG"
     gene            1191..2012
                     /gene="rhaD"
                     /locus_tag="ACZO9R_05460"
     CDS             1191..2012
                     /gene="rhaD"
                     /locus_tag="ACZO9R_05460"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HIR0912545.1"
                     /translation="MKFLDAEFVQGFIRMANDGWEQGWHERNGGNLSYRVKPEEVEAV
                     RENFQEKEWQPIGTTVPNLAGEYFLVTGSGKYFRNVILKPEDSICMIELDEKGENYRI
                     VWGLVNGGRPTSELPSHLMNLEVKKLQNPKYRVVYHAHTTNIIALTFVLPLEDRIFTR
                     ELWEMATECPVVFPAGVGVVPWMVPGGREIAVATSELMKKYDLAIWAHHGMFAAGEDF
                     DLTFGLMHTAEKSAEILVKTLSMRPDKLQTIQPDDFRHLAEDFHVTLPEEFLYEK"
     gene            complement(2134..2331)
                     /locus_tag="ACZO9R_05465"
     CDS             complement(2134..2331)
                     /locus_tag="ACZO9R_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912546.1"
                     /translation="MIVAIYYDSVFVLPVHPVNRIDRLAPESRKQQRSKQQPSSSFAT
                     LLHQAMTVTEEDDVHSFDATA"
     gene            2496..>3424
                     /locus_tag="ACZO9R_05470"
     CDS             2496..>3424
                     /locus_tag="ACZO9R_05470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR0912547.1"
                     /translation="MSLQFVFGNSGSGKSDYLYQSILEEAEREPEKNFLLLVPEQFTM
                     QTQRELVCRQPNHAIMNVDVLSFVRLAYRVFDDLGMQDLVILEETGKNLVLRKVAELK
                     KKELSVLGGNLNKMGYIGEIKSLISEMAQYNITPEDLGAFLGGQDVGDTLRCKMQDIL
                     TMYEGFREYIDGKYITAEEILTLLCEVAEESELIRDSVIVFDEFTGFTPIQNRLLRVM
                     LPLADRVIVSLSMDIREDFYHSRGVHELFSMSKETVQTLLKIAADAGCEVLSPVIMEP
                     GEHRRYENAPELFFMEQNLFRPMYRKWPKPVND"
BASE COUNT          897 a          742 c         1009 g          776 t
ORIGIN      
        1 tggcagctgg acgatgtgat cggatttgac aatgacggcg gactcacagg tggaatccag
       61 acgaccggaa actatccggg gcgcgccaga acgccgcagc agctgatgga ggacatggag
      121 gaggcaatga agctgatgcc gggaacggca aaattaaata ttcatgcatc ctatgcgatt
      181 tttgaaccgg gagaatttgc ggaccgcgac gccttagagc cgaagcattt cgcaaagtgg
      241 gtggaatttg cgaggaagca tcatatggga attgatttca acccgacatt tttctcccac
      301 gaaaaagtaa aagacggact taccttatca agccccgatg aggagaccag aaacttctgg
      361 atcaaccacg gaaaggcatg catccgcatc tcggaatatt ttgcaaagga gaccggtgtg
      421 ccgtgcgtca tgaatatctg gacaggagac ggattcaagg atgttccggc agaccgtatg
      481 ggaccgcgtc tgcgctacaa ggattcgatc gagcagattt tatctgagcc gtatgacagg
      541 aagcttgtca agccgtgcgt ggaatccaag gtattcggta tcggcgtgga gtcctacacc
      601 gtgggttcgg cagaatttac gttgagtttc gccgcacagc atgaaggctg catgccgctc
      661 atggacaacg gacattatca tccgacggag tatgtgtcgg acaagattcc tgccatgctc
      721 tgtttttacc cggagtttgc acttcacatt accagaggcg tgcgctggga ttcggatcat
      781 gtgctgctct tagatgacga gacgaaagag atggcaaaag agatcgtaag aaatgacgcg
      841 cttgaccgtg tatacatggc gttagattac tttgacgcga gcatcaaccg tgtcctggcg
      901 cttgcgaccg gcttcaggag ctggcagaaa gccctgttaa cggcactgtg cacgccgaat
      961 gagatgttaa aggaactgca ggatacgaat cagctgggaa aactcatggt tatgcacgag
     1021 gcggtaaaaa tgcttccgat cggcgagatc tgggaggagt actgccgcag ggaaggcgtg
     1081 tgcgatgacc ttcatttcta cgatgagatc gagcgttatg agcgtgaggt actgaccaaa
     1141 agaggataaa cagatagaag ctgaatcaga gagaagagag gaagagaatc atgaaatttt
     1201 tggatgcaga atttgtacag ggatttatcc gcatggcaaa cgacggatgg gagcagggct
     1261 ggcatgagag aaacggcgga aacttatctt accgcgtgaa accggaggaa gtggaagcgg
     1321 tgagggagaa ctttcaggaa aaggagtggc agccgatcgg caccacggtg cccaatctgg
     1381 caggagagta tttccttgtg accggaagtg gaaaatattt ccgcaacgtc atcttaaagc
     1441 cggaagattc gatctgcatg attgagctgg acgaaaaagg cgagaactac cggatcgtgt
     1501 ggggactggt gaacggcgga agaccgacct cggaactgcc gagccatctg atgaacctgg
     1561 aagtaaagaa actgcagaat ccgaagtacc gtgtggtcta tcacgcacat accaccaata
     1621 tcattgcgct gacgttcgtg cttccactgg aggaccgcat tttcacgaga gaactctggg
     1681 agatggcaac ggaatgtccg gtggtattcc cggcgggtgt cggcgtggtg ccgtggatgg
     1741 ttccgggcgg cagggagatt gcggttgcga ccagcgagct gatgaaaaaa tatgatcttg
     1801 cgatctgggc gcatcacggc atgtttgctg ccggcgagga ctttgactta acgttcggtc
     1861 tgatgcacac cgcagaaaaa tcggcggaga ttctggtgaa gacgctctcc atgcgtccgg
     1921 ataagctcca gacgatacag ccggacgact tcagacatct ggcagaggat tttcatgtga
     1981 ccctgccgga agaattcctg tatgaaaaat aaaagagaag cctgcctgca ggcataagaa
     2041 aacccccggg cgcaatggaa aggtacgaaa ccttggattg tcaaccgggg gccttcttat
     2101 gaaattcttt tgtctgattc atagaacctt tctctaggca gtggcatcga acgaatgaac
     2161 atcatcctcc tctgtcaccg tcattgcctg atgcagcagt gtggcgaacg aagaagaagg
     2221 ctgctgtttg gaacgttgct gctttctgct ctctggtgca agccggtcga tccggtttac
     2281 tgggtgaacg ggtaacacga aaacactatc atagtagatt gcgacaatca tcctccttga
     2341 caaataaatc acaactgata ggggttaatg agttccttct ctactatata tatcggctga
     2401 catcagaaat aattaagaga aaatagaggg aatattattt ggaagaagcg tgcacctgtg
     2461 ttaaaatatt gctgtgaaca gggggcacgt tttttatgtc attacagttt gtgttcggca
     2521 attccggaag cggaaagtcc gattatttat atcagtcgat attggaggaa gcggaaaggg
     2581 aaccggagaa gaatttcctt ttgcttgtcc cggaacagtt tacgatgcag acgcagcggg
     2641 aactcgtgtg ccgccagccc aaccatgcca tcatgaacgt ggatgtgctc agttttgtgc
     2701 gcctggcgta ccgtgtgttc gatgacctcg gaatgcagga tctggtcatt ttagaggaga
     2761 cgggcaagaa tctggtgctg cgcaaggtgg cggagcttaa gaaaaaagaa ctctcggtgc
     2821 ttggcggcaa tctcaacaaa atgggataca tcggtgagat caagtccctg atctcggaga
     2881 tggcgcagta caatatcacg ccggaggatc ttggtgcttt tctcggcggg caggatgtcg
     2941 gggataccct gcgctgtaag atgcaggata ttcttacgat gtatgaggga ttccgggagt
     3001 atattgacgg gaaatacatc acggcggagg agattctgac actcttgtgt gaggtggcgg
     3061 aggaatccga gctcatcagg gacagtgtga ttgtctttga cgagtttacg ggattcaccc
     3121 cgatccagaa ccggctgctt cgggtgatgc tgccgcttgc cgatcgcgtc atcgtgtcgc
     3181 tctccatgga tatccgggag gatttttacc acagcagagg tgtgcacgag cttttttcca
     3241 tgagcaagga gaccgtgcag acactgctta agattgcggc ggatgcggga tgcgaggtac
     3301 tctctccggt gattatggag ccgggagagc acaggcgcta tgaaaatgca ccggagctgt
     3361 ttttcatgga acagaatctg ttccgtccga tgtaccggaa gtggccaaag ccggttaatg
     3421 acat
//
LOCUS       DBMDQJ010000211         3419 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10079_length_3419_cov_1.750151, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000211 DBMDQJ010000000
VERSION     DBMDQJ010000211.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10079_length_3419_cov_1.750151"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="ACZO9R_05475"
     CDS             <1..1004
                     /locus_tag="ACZO9R_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR0912548.1"
                     /translation="WEKETRPFLRSREFLWQEGHTAHATAEEAEQRTIQMLNLYADFC
                     EEVLAMPVIRGRKTEKEKFAGAEATYTIEALMHDGKALQSGTSHNFGDGFAKAFGVQY
                     TDKDNKLQYVHQTSWGMTTRLIGAIIMVHGDDSGLVLPPRIAPVQAMVIPIQQYKDGV
                     MDAAKSVCERLGKVCRAKLDDSDKSPGWKFSEQEMRGIPVRIELGPKDIEAGKCVVVR
                     RDTREKIEVALEDLEQKIPEILETIQKEMFERAKAHRDAHIWDAHNYEELKDIAANKP
                     GFIRGMWCEDQACEDKIKEELAVTSRCIPFHDQEQISGTCVCCGRPAHKLVYWGKAY"
     gene            1015..2373
                     /locus_tag="ACZO9R_05480"
     CDS             1015..2373
                     /locus_tag="ACZO9R_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0052929 - ATP:3'-cytidine-cytidine-tRNA
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR0912549.1"
                     /translation="MDKNAKISLPAKVARILNTLGGHGYEAYAVGGCVRDSLLGRMPQ
                     DWDITTSARPEQVKALFRHTIDTGIQHGTVTVMLDHEGFEVTTYRIDGEYEDARHPKE
                     VAFTANLLEDLKRRDFTINAMAYNEVTGLVDAFDGIGDLERGVIRCVGNAMERFSEDA
                     LRMLRAVRFAAQLGFSIERETQEAIAGLAGNIKKVSAERIQVELVKLLTSPNPGELKV
                     AYRTGLTAVFLPEFDMMMETPQNNPHHCYSVGEHTLKAIEAVPADKVLRLTMLLHDIA
                     KPACKTTDEKGVDHFYGHPVRGSEMARGILRRLKFDNDTTDRVCRLVHWHDDNPELSG
                     KAVRRAMHRIGVEQYPALFAVKRADILAQSGYRRAEKLAYVDAYEQMYRECMEKKECL
                     TIRQLAVSGQDLIAAGMQPGKQIGEMLNELLERVLEDPGLNTKEQLLKIVTGKISDQN
                     RS"
     gene            2446..>3419
                     /locus_tag="ACZO9R_05485"
     CDS             2446..>3419
                     /locus_tag="ACZO9R_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CotS family spore coat protein"
                     /protein_id="HIR0912550.1"
                     /translation="MYNRPEQVLEQYDLEVRAVSKGRESYICDTTQGQLLLKEYKGSA
                     ERAEFLSAILGHLGGQGLLTEQVVRTKEDAPIAVVEDETKYMVLTAVSGSECDTRNRA
                     DMIAGAEKLAMLHNAAETYSETVPEFVCNDPNELQELYEKHNRELVKVRNYIRSKKKK
                     NDFEVLFYGQYERFMEKARQVAQELSAMGQGGQSAGFCHGDYNQHNILFSKTGIGIVH
                     FECFSYQIQVSDLANYMRKMLEKNNWNTGLGMDLICAYDRVRRLTAVELNYLYLYMAY
                     PEKFWKIANRYYNTHKAWVSGRNIEKLEKFIRQEDERERFLEMMRHFT"
BASE COUNT          903 a          750 c         1078 g          688 t
ORIGIN      
        1 gctgggagaa ggagacgaga ccgttcctcc gttccaggga gtttctgtgg caggagggac
       61 acaccgcgca tgcgaccgca gaggaagccg agcagcgcac gattcagatg ctgaatctgt
      121 acgcggactt ctgcgaggag gtactggcaa tgccggtcat ccgcggacgc aagaccgaga
      181 aggagaagtt tgcaggtgcg gaggctacct atacgattga ggcgttgatg catgacggaa
      241 aggcactgca gtccggtacc agccataact tcggcgacgg attcgcaaaa gcgttcggtg
      301 tccagtatac cgataaagat aataagcttc agtatgtaca tcagacttcc tggggtatga
      361 ccacacgtct cattggtgcg atcatcatgg tacacggcga tgattcgggt ctggttctgc
      421 cgccgagaat tgcaccggta caggcgatgg tgattccgat ccagcagtac aaagacggcg
      481 ttatggatgc cgcaaagagt gtctgcgagc gccttggaaa agtatgccgc gcgaagctgg
      541 atgactcgga caagagcccg ggctggaagt tctccgagca ggagatgcgc ggaattccgg
      601 ttcgtatcga acttggacca aaagacatcg aggcaggcaa gtgcgtagta gtgcgcagag
      661 acaccagaga gaagattgag gtcgcactgg aggatctgga gcagaagatt ccggagattt
      721 tagagaccat ccagaaagag atgttcgagc gtgcaaaggc acaccgcgac gcgcatatct
      781 gggatgccca caattatgaa gaattaaagg acatcgcggc aaacaaaccg ggatttatcc
      841 gcggtatgtg gtgcgaggat caggcgtgcg aggacaagat caaagaggag cttgccgtga
      901 catcccgctg tatcccgttc catgatcagg agcagatttc cggaacctgc gtatgctgcg
      961 gcagaccggc acacaagctg gtatactggg gcaaggcata ttaaggagaa gtgcgtggat
     1021 aaaaatgcaa agatcagcct gcctgcgaag gtcgcgagga ttctgaatac attaggtggg
     1081 cacggatatg aagcgtatgc ggtcggaggc tgcgtgcgcg actccctgct cgggagaatg
     1141 ccgcaggact gggacattac gacctcggcg cgaccggagc aggtcaaggc tcttttccgg
     1201 cacacgattg ataccggaat ccagcatggg acggtgacgg tcatgctgga tcatgaggga
     1261 ttcgaggtca caacctaccg gattgacgga gaatacgagg atgcgaggca cccgaaggaa
     1321 gtggcgttta ccgcgaatct tctggaggat ctgaaacgcc gcgattttac gatcaatgcg
     1381 atggcctaca atgaagtgac cggactggtg gatgcctttg acggaatcgg cgatctggag
     1441 agaggcgtga tccgttgcgt gggaaatgcc atggagcgct tttcggagga cgcccttcgc
     1501 atgctccggg cggttcgttt tgcagcgcag cttggctttt cgattgaacg ggagacacag
     1561 gaagccattg ccggactggc gggcaatata aaaaaggtca gtgcggaacg gattcaggtc
     1621 gaactggtaa agcttttgac ttcgccgaat ccgggagagc tgaaagtggc gtatcgcacg
     1681 gggcttacgg ctgtttttct gccggagttt gacatgatga tggagacacc gcagaacaat
     1741 ccgcatcact gctacagtgt gggagagcat acgctaaagg cgattgaagc agtgccggcg
     1801 gataaggtgt taaggcttac gatgctgctg catgacattg caaagcctgc ctgcaagacg
     1861 acggacgaaa aaggcgtgga tcatttttac ggacatccgg tgcgcggaag cgagatggcg
     1921 cgtgggattc tgcgccggct gaaatttgac aacgatacca ccgaccgggt gtgccggctg
     1981 gtgcactggc acgacgataa tccggagctt tctgggaagg cggtccgcag ggcgatgcac
     2041 aggattggcg tggaacagta cccggcattg tttgcggtca agcgcgcgga tattctggcg
     2101 cagagcgggt accggcgcgc ggaaaagctg gcgtatgtgg acgcatatga acagatgtac
     2161 cgggagtgca tggagaaaaa ggagtgtctt acgatccggc aactggcggt ttccggtcag
     2221 gatctgattg cagccggaat gcagccggga aagcagatcg gggagatgct gaatgaactg
     2281 ctggaacggg tactggaaga tcccgggctg aatacgaagg aacaattatt aaaaattgtg
     2341 acaggaaaga tttctgatca aaacaggtca tgaaataaaa acccctcaca tatgatgaag
     2401 agacacggca gagcagccgc agatcaatca tatggagggg ttttcgtgta taatcgaccg
     2461 gagcaggtat tggagcagta cgacctggaa gtgagagcgg tttccaaagg gcgtgaaagt
     2521 tatatttgtg atacaacaca ggggcagtta cttttaaaag aatacaaagg ctcggcggag
     2581 cgggcggagt ttctctctgc catactcggg catctgggtg gacagggact cttaacggag
     2641 caggtcgtgc ggacgaagga ggacgccccg attgccgtcg tggaggatga gacaaagtat
     2701 atggtattga cggctgtttc cgggagcgag tgtgacacca gaaaccgggc ggacatgatc
     2761 gcgggggcgg agaaactggc aatgctgcac aatgcggcgg aaacttattc ggagacggtt
     2821 ccggagtttg tctgcaacga cccgaatgaa ctgcaggaac tgtatgagaa gcataaccgg
     2881 gaactggtca aagtccggaa ttacatacgg tccaaaaaga aaaagaatga ttttgaagtg
     2941 ctcttttacg ggcagtatga gcgctttatg gaaaaagcaa ggcaggtggc acaggaactt
     3001 tcggcgatgg gacagggcgg gcagagcgcc gggttttgtc acggcgatta taaccagcac
     3061 aatattctgt tttccaaaac cggaatcggc attgtccatt ttgaatgctt ttcctaccag
     3121 atacaggtca gtgatctggc aaattatatg cggaaaatgc tggaaaaaaa caactggaac
     3181 accggacttg gcatggatct catctgtgcg tatgaccgtg tgcgccggct gactgccgtg
     3241 gaactaaact atctgtatct gtatatggca tatccggaaa aattctggaa aattgcgaac
     3301 cgctactata acacacacaa ggcatgggtg tccggacgca atattgaaaa gctggaaaaa
     3361 ttcatacggc aggaggatga gcgggaacga ttcctggaaa tgatgcgcca tttcacctt
//
LOCUS       DBMDQJ010000212         3415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10100_length_3415_cov_1.019312, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000212 DBMDQJ010000000
VERSION     DBMDQJ010000212.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10100_length_3415_cov_1.019312"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="ACZO9R_05490"
     CDS             <1..513
                     /locus_tag="ACZO9R_05490"
                     /inference="COORDINATES: protein motif:HMM:NF019305.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HIR0912551.1"
                     /translation="CGGYQMMGRTVSDPDNTEGGGSLRGMNLLPIDTVFCPSKTTTQT
                     RGTLLEIDGVLSDLSGLAVEGYEIHMGETVRDASAKPLVRLLRRDGEIEDGCQTENAF
                     GTYLHGVFDAPKAALRTAQALAKKKGVTLTSEALDTHAYKEQQYDKLAATLRKYLDLD
                     AVYKMMREYE"
     gene            553..1599
                     /gene="cobT"
                     /locus_tag="ACZO9R_05495"
     CDS             553..1599
                     /gene="cobT"
                     /locus_tag="ACZO9R_05495"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HIR0912552.1"
                     /translation="MRQLKIEPPDQVVYKEVLGNWDALAKPLDGMGEFEELFARIGAI
                     RRDPALDISRKAVVVMCADNGIVEEKISQSGQDVTAKVAAAMGRGTSSVCRMAKAAGI
                     EVIPVDIGINEEGSPEGVLPCKVRRGTRNFIKERAMTEQETLAAIEIGMELAKRLAHE
                     GYKLLATGEMGIGNTTTSSAVAAALLSCDPKEITGKGAGLSDTALLRKIAVVEEGIQM
                     HELYQADAFDVLCAVGGLDIAGLSGVFIGGALAHVPVVVDGVISAVAALAAERMIPGV
                     RDYVIPSHKSREPAAERILEELGIRPVLDAGLALGEGTGAVMMCGLLDIARTIYGERT
                     TFADIDVAPYHRFG"
     gene            1619..2215
                     /locus_tag="ACZO9R_05500"
     CDS             1619..2215
                     /locus_tag="ACZO9R_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR0912553.1"
                     /translation="MILVIGGSGSGKSAYAEKVALECQKEKRHFYLATMHVFGEEGQK
                     KVERHKRMRAGKGFVTIEQPTDIMQCLPALREAGGGVVLLECMSNLAANEMFDTEEPA
                     GSEQVAEKIVTQIGVLAQETEELVIVTNNVFEDGIVYDASTMEYLRALGSINVRLAER
                     SDVVVEVIAGIPVARKGKLPVAAAEKQGHRQKGAPACP"
     gene            2206..2970
                     /locus_tag="ACZO9R_05505"
     CDS             2206..2970
                     /locus_tag="ACZO9R_05505"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HIR0912554.1"
                     /translation="MSVIKSFFIAFSIYSKIPVPQFAWKEEDMRYTLCFFPWVGAVIG
                     LCFLLWNRFAGVYGIPSTARAAVGAVLPLAISGGFHADGFMDTMDALHSYRDRERKLE
                     ILKDSHIGAFAVICFVMYELIYLGAVSMITEKEQILVLAAGFFLSRTLSGIGVVSLRC
                     AKTNGMLYQFASEAHRRVVLTALYLQLVLCVGLMLAVSVKTGALACAAAGLTYAYYRQ
                     KSYREFGGITGDLAGYFVTLCEGVMAMLVAAAGLIG"
     gene            3012..3398
                     /locus_tag="ACZO9R_05510"
     CDS             3012..3398
                     /locus_tag="ACZO9R_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HIR0912555.1"
                     /translation="MELYIGGYAQGKLTYVTDRHPGEKHPVWDGATETFTGRTLKEAP
                     VCNHYHLWVKRLLKDGADVRALTGQLLLEHPDCIIISDEIGNGIVPMEHEEREYRELT
                     GRLLCELANKADRVERIVCGIGERIK"
BASE COUNT          778 a          768 c         1189 g          680 t
ORIGIN      
        1 tgcggcggct accagatgat gggacgtacg gtctccgacc cggacaatac cgagggcggc
       61 ggcagtctgc gcggcatgaa cctgctgccg atcgacacgg tgttctgccc gagcaagacc
      121 acgacgcaga cgcgcggcac gctgctggag attgacggcg tgctgtccga tttgagcggt
      181 ctggcggtcg agggctatga gattcacatg ggcgaaaccg tgcgcgatgc gagtgcaaag
      241 ccgctcgttc gattgcttcg cagggacggt gaaatcgagg acggctgtca gacggaaaac
      301 gccttcggca cttatctgca cggtgtgttc gatgcgccga aggcggcgct gcggacggcg
      361 caggcactgg cgaaaaagaa gggcgtgacg ctgaccagcg aagcactcga tacgcacgcc
      421 tacaaggagc agcagtacga caagctggcg gcaacgctgc gcaagtatct ggatctcgat
      481 gcggtctata agatgatgcg ggaatatgag tgacaaaaga gagaggtgtg tgggatgtta
      541 gacagacagc agatgagaca gttgaagata gagccgccgg atcaggtggt ctataaggaa
      601 gtcctcggca actgggatgc gcttgcaaag ccgctggacg ggatgggaga atttgaggaa
      661 ttgtttgccc gcatcggagc aatccggcgt gatcccgccc ttgatatttc cagaaaagca
      721 gtggttgtga tgtgtgcgga caacggaatt gtggaggaga agatcagcca gtccggacag
      781 gatgtgacgg cgaaggtggc ggcagccatg gggagaggga cgagctctgt ctgccggatg
      841 gcaaaagcag ccggcatcga ggtaataccg gtggacatcg gaatcaacga agagggatcg
      901 ccggagggcg tgctgccgtg caaagtgcgt cgcggaacca gaaattttat caaggaacgg
      961 gcgatgacgg agcaggagac actggcggcg attgagattg gcatggagct tgcgaagcgg
     1021 cttgcacacg agggctataa gctgcttgcg accggtgaga tgggaatcgg caacacaacg
     1081 accagcagtg cggtggcggc ggcactgtta tcctgcgatc cgaaggagat caccggaaag
     1141 ggagccggac tcagtgacac ggcgctttta cgcaagattg cggtggtcga agagggaatc
     1201 cagatgcatg aactctatca ggcggacgca ttcgatgtgc tgtgcgcagt gggcggactg
     1261 gatattgccg gactttccgg cgtgtttatc ggcggtgccc tggcacatgt gccggtggtt
     1321 gttgatggag tcatcagtgc ggtggctgcg cttgcggcgg agcggatgat tcccggcgtg
     1381 cgcgattacg tgataccatc gcacaagagc agggaaccgg cggcggagcg catactggaa
     1441 gaactgggaa tccgcccggt gttagatgcg ggactggcgc tgggagaggg aaccggagct
     1501 gtgatgatgt gcggactgct ggacattgcg cggacgatct acggcgagcg caccacgttt
     1561 gcggatattg atgtggcacc gtatcacaga ttcgggtagc ctcagagagg agtaggacat
     1621 gattctggtg atcggcggaa gcggcagcgg aaagtcagct tatgcggaaa aagtggcatt
     1681 ggagtgtcaa aaagagaaaa ggcacttcta cctggcaacc atgcatgtgt tcggcgagga
     1741 ggggcaaaaa aaggtggagc gccataaaag gatgcgcgca ggcaaaggct ttgtgacgat
     1801 cgagcagccg acggacatca tgcagtgcct tcccgcactt cgggaggccg gtggcggcgt
     1861 ggtgctcctg gagtgcatgt cgaatctggc tgccaatgaa atgtttgaca cggaagaacc
     1921 ggcgggcagt gagcaggtcg cggaaaagat tgtcacgcag attggcgtgc tggcgcagga
     1981 gacggaagaa cttgtcattg tcaccaacaa tgtgtttgag gacggcatcg tctatgacgc
     2041 ttccaccatg gaatatcttc gggcgctcgg cagcatcaat gtgagactgg cagagcgttc
     2101 ggacgttgtg gtggaagtga ttgcgggaat tcccgttgca agaaagggaa agcttccggt
     2161 agcggcggca gagaaacagg gacacagaca gaaaggagca ccggcatgtc cgtgataaag
     2221 tcttttttta tagcattttc gatttattct aaaattccgg tgccgcagtt cgcgtggaag
     2281 gaggaggaca tgcgctatac gctgtgcttc ttcccgtggg tcggagcggt gatcggattg
     2341 tgtttcctgt tgtggaaccg gtttgcgggc gtgtatggga tcccgtccac tgcccgggcg
     2401 gcggtcggag cggttctgcc gcttgcaata tccggcggat ttcatgcgga cgggtttatg
     2461 gacaccatgg acgcgctgca ttcttaccgc gacagggagc gcaaactgga gattttaaaa
     2521 gattcccaca tcggcgcgtt tgccgtgatc tgttttgtga tgtatgagct gatctatctg
     2581 ggagcagtgt ccatgattac ggagaaggaa cagattctgg tacttgccgc aggctttttt
     2641 ttgtcgcgca ccttaagcgg gatcggtgtc gtatccctgc gctgtgccaa gacgaacggg
     2701 atgctctatc agttcgcgag cgaggcgcac cggcgggtgg tgctaacggc gctgtatctg
     2761 cagcttgtac tctgcgtcgg gctgatgctt gccgtatcgg tgaaaaccgg tgctcttgcg
     2821 tgtgcggcag cagggcttac ctacgcgtac tacagacaga agagttaccg ggaatttggc
     2881 ggtattaccg gagacctcgc ggggtatttt gtgacgctgt gcgagggtgt gatggcgatg
     2941 cttgtggcgg cggcagggct gatcggctag gaatgaagca ttgcgggatg acgcatcaga
     3001 agggataaca catggaacta tacattggcg gatatgcaca gggaaagctt acttatgtca
     3061 cagacaggca tccgggggaa aaacatccgg tgtgggatgg agccacagag acatttaccg
     3121 ggagaacatt gaaggaggcg ccggtctgca accactatca tctgtgggtg aagaggctgc
     3181 ttaaggatgg agcggatgta agagcgctga ccgggcagct tcttttggaa cacccggact
     3241 gcatcatcat cagtgatgag atcggcaacg ggatcgtgcc gatggagcat gaggagcgcg
     3301 agtaccggga gctgaccggc aggctgctgt gtgaactggc aaataaggca gaccgcgtgg
     3361 agcgcattgt gtgcgggatc ggagagcgga tcaaatgagg tatgaggcag acatg
//
LOCUS       DBMDQJ010000213         3407 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10125_length_3407_cov_1.599819, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000213 DBMDQJ010000000
VERSION     DBMDQJ010000213.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10125_length_3407_cov_1.599819"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="ACZO9R_05515"
     CDS             complement(<1..463)
                     /locus_tag="ACZO9R_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR0912556.1"
                     /translation="MNSKRSSYRGFGIYLLLILAIIGIWYWMDGNTSTNSYTRAQFET
                     AVADGNVAQLKIVPNRGEVPTGNVYITFKDSSTQVLAVNDVNDIEDYLRDQQFTSYTV
                     ENPPEQSWIVTILPYLIIFAAMFIFFMIMTNQAAANSGGGGKMMNFGKSRAK"
     gene            complement(476..1006)
                     /gene="hpt"
                     /locus_tag="ACZO9R_05520"
     CDS             complement(476..1006)
                     /gene="hpt"
                     /locus_tag="ACZO9R_05520"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR0912557.1"
                     /translation="MKIKKINVHLTEEQIEQRVRELGAEISKVYGDEPVCLICILKGS
                     VFFTCELAKRITSPVEIEFMSVSSYGSGTSSSGVVRIVNDLGTSIEGKNVLVIEDIID
                     SGRTLSYLLENLKTRNPKTLRLCTLLDKPDRRVVDVNVDYVGFVIPDEFVVGYGLDYD
                     QKYRNLPYIGFVEIEE"
     gene            complement(987..>2228)
                     /gene="tilS"
                     /locus_tag="ACZO9R_05525"
     CDS             complement(987..>2228)
                     /gene="tilS"
                     /locus_tag="ACZO9R_05525"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR0912558.1"
                     /translation="DVLMREMRSSAIFLGAILARCGQADLSYPGGCELGPRPIDLHLA
                     GLRDLGAEIREEGGFLHCRADRLRGREVVLRMPSVGATENLMLAACGAEGVTVIRPLL
                     GMERAEIEAYLEAQGQPYCRDATNEDTDYSRNRIRHKVMPELKAVNTGAVHHMMQSAQ
                     MLMELSDYLEEETRRATERVCRDTPDACVIDAVLFSEYPALLIREVLLVTLQKVAGSS
                     RDIGSVHVEELCTLYTRQVGRVICLPYGVRAERVYEGIRLYREEPAADMERESIEVTG
                     EMLSRAEKEEVVLALPDGRLHLRIRDFSGNMQEIPKKTYTKWFDYGKIKSGLRLRRRE
                     SGDYLKIDAAGHTKKLKEYFVNEKIPAAKRDAIWLLATGSEILWVAGGRIGAGCKVGE
                     NTEKILEVRLSGGNDCEDQEN"
     assembly_gap    2229..2238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2239..2702)
                     /gene="tilS"
                     /locus_tag="ACZO9R_05530"
     CDS             complement(<2239..2702)
                     /gene="tilS"
                     /locus_tag="ACZO9R_05530"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR0912559.1"
                     /translation="MQKKGMELEGLVKSGDVVLAGVSGGADSVCLLLMLLEYRKHCDF
                     LLEAVHVEHGIRGEASRRDAAFVKRLCEKRGVCCRIYPVDVPAYAKGHGLGVEEAARK
                     LRYECFREAAEDYDGRPVKIALAHHADDNAETMLFRLIRGSGLHGLYGMRPAR"
     gene            complement(2733..>3407)
                     /locus_tag="ACZO9R_05535"
     CDS             complement(2733..>3407)
                     /locus_tag="ACZO9R_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR0912560.1"
                     /translation="LIYEDTAYRSVQGIARVKKDEAKISGDNFSFLELERGEMLLGLS
                     DGMGSGSSACKESEMVLDLVERFLEAGFSLETAIRMMNSAMVMKGENDIYSTLDLCMV
                     NLYSGMARLYKVGAAAAFIRREDEVECIASENLPVGARAHLDAKPREIQLSDGDFIVM
                     VTDGVLEYLHVPKPEETMREMILSIKTNNPGILAKKIMDRVMLFTGGRAQDDMTVLAA
                     CIWKKA"
BASE COUNT          723 a         1127 c          708 g          839 t
ORIGIN      
        1 gctttgcgcg gctctttccg aagttcatca tcttaccgcc gccgccggaa ttggcagccg
       61 cctgattggt cataatcatg aagaaaatga acatcgccgc aaaaataatc agatacggaa
      121 gtatcgtcac gatccagctc tgctccggcg gattctccac ggtgtaagac gtaaactgct
      181 gatcgcgcag atagtcttct atatcattta catcgtttac cgccagaacc tgtgtcgagg
      241 agtccttaaa tgtaatgtat acattaccgg tcggcacctc gccgcggttc ggtacaatct
      301 tcagctgtgc aacatttcca tctgcaaccg cagtctcaaa ctgtgcccgc gtgtaactgt
      361 tggttgacgt gtttccgtcc atccaatacc agatacctat gatggcaagt atcaggagca
      421 gatagatgcc aaatcctctg taactgcttc tcttactatt caatcgattt ctcctttatt
      481 cctctatctc aacaaatccg atataaggca gattgcggta cttctggtca tagtcgagac
      541 cgtatcctac cacaaattca tccggaatga caaatcccac ataatccaca ttgacatcca
      601 ccactctccg gtccggctta tccagaagcg tacacagtct gagtgtcttc ggatttcttg
      661 tcttcaaatt ctccagcaaa tagcttaaag ttctgccgga atcgatgatg tcctcaatta
      721 ccagcacatt cttgccttca atactggttc caagatcatt tacgatgcgc accacaccgc
      781 tggaacttgt gcctgatcca tagctggata ccgacataaa ctctatctca accggtgaag
      841 taatacgctt tgccagttcg caggtaaaaa ataccgatcc cttcaaaata cagatcaggc
      901 ataccggctc atctccgtac accttgctga tctcggctcc caattctctt actctctgct
      961 caatctgttc ctctgtgaga tgaacgttaa ttttcttgat cttcacaatc gtttcctccg
     1021 cttaaccgta cttctaatat cttttctgta ttctctccga ctttgcagcc ggcgccgatg
     1081 cgcccgcctg ccacccacag gatctcggac cccgttgcca gaagccatat agcgtctctc
     1141 ttcgccgccg gaatcttctc attgacaaaa tattccttca gcttcttggt atgtccggca
     1201 gcatctatct tcaggtaatc gccgctctct cttctgcgca gccgcagacc agattttatt
     1261 ttaccataat cgaaccattt cgtatatgtt tttttcggga tttcctgcat attccccgaa
     1321 aaatcacgta tccgcaggtg cagcctgcca tccggcagcg caagcaccac ctcttccttt
     1381 tctgcgcgcg aaagcatctc cccggtaacc tcaatgctct cccgttccat atcagccgcc
     1441 ggctcttccc tgtacagccg gattccctca tacacacgct ccgcacgcac gccgtagggc
     1501 aggcagatca cacggccgac ctgtcttgta tacagtgtgc acagttcttc tacatgcaca
     1561 cttccgatgt cacggctgct gcccgccact ttttgcagcg tcacaagcag tacttcccgg
     1621 attaaaagtg cgggatattc tgaaaaaaga accgcatcga tcacacaggc atccggggtg
     1681 tcacggcaca cacgctccgt cgcccggcgc gtctcttcct ccaggtaatc cgacaattcc
     1741 attaacatct gtgcgctctg catcatatga tgcaccgccc ccgtattcac cgcttttaat
     1801 tccggcatca ccttatggcg aatccggttt ctgctgtagt cggtatcctc attggtcgca
     1861 tcccggcagt aaggctgtcc ctgtgcctcc agatacgcct cgatctccgc tctctccatt
     1921 ccaagcagcg gccggatcac cgtgacgccc tccgccccgc aggcggccag catcagattc
     1981 tccgtggccc ccacggatgg catccgcagc accacttccc ggccccgcag ccggtccgcc
     2041 cggcagtgga ggaagccgcc ctcctcccgg atctccgccc ccaggtcccg caggcccgca
     2101 aggtggaggt cgataggccg ggggcccagc tcgcacccgc cggggtagct caggtccgcc
     2161 tgcccgcacc gggccaggat cgcccccagg aaaatggcgg aggagcgcat ctcccgcatg
     2221 agtacatcnn nnnnnnnncg cgccgggcgc ataccgtaga gtccgtggag tccgctcccc
     2281 cggatcagcc ggaacagcat ggtctccgca ttatcatccg catgatgcgc gagcgcgatc
     2341 ttcaccggtc tgccgtcata gtcctctgct gcctccctga agcactcata gcggagcttc
     2401 ctcgccgcct cctcaacgcc gaggccgtgt cccttggcat acgccgggac atccaccgga
     2461 taaatcctgc agcaaacgcc ccgcttttcg caaagccttt taacaaacgc ggcgtctctc
     2521 ctgctggcct ctccccggat cccgtgttcc acgtgcaccg cctccaggag aaaatcgcag
     2581 tgcttccggt actcaagcag cataagcagc agacataccg agtccgctcc tccggagacg
     2641 cccgcaagca ccacatcccc ggatttaaca agcccctcca gttccattcc tttcttctgc
     2701 atcgcatttc ctctatcgtt tcacattcta ttctatgcct ttttccagat gcacgccgca
     2761 agtaccgtca tatcgtcctg tgcgcgcccg ccggtaaaaa gcatcactct gtccataatc
     2821 ttttttgcca gaattcccgg attgttggtt ttgatactga gaatcatttc ccgcatcgtc
     2881 tcctccggct tcggaacatg taagtattct aacacaccat ccgtcaccat gacaataaaa
     2941 tcaccgtcac ttagctgtat ctccctcggt tttgcatcca ggtgcgcacg cgcccccacc
     3001 ggcagatttt ccgatgcaat gcactccacc tcatcctccc gccggataaa tgcagccgcc
     3061 gctcccacct tgtacaggcg tgccataccg ctgtacaggt tcaccatgca gaggtctaac
     3121 gtggagtaga tgtcattttc ccctttcatc accatcgccg aattcatcat gcgtattgcc
     3181 gtctccaggg aaaaccctgc ttccagaaaa cgttccacaa gatccagcac catctcgctc
     3241 tccttgcagg cactgctccc ggagcccatg ccgtccgaca gtccaagcag catttcccct
     3301 cgctcgagtt ccagaaaaga aaagttgtcg cctgaaattt ttgcctcatc cttcttcacc
     3361 cgcgcaattc cctgcacgct ccggtatgcg gtgtcctcgt agatcag
//
LOCUS       DBMDQJ010000214         3402 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10144_length_3402_cov_1.280218, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000214 DBMDQJ010000000
VERSION     DBMDQJ010000214.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3402)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3402)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3402
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10144_length_3402_cov_1.280218"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="ACZO9R_05540"
     CDS             complement(<1..742)
                     /locus_tag="ACZO9R_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR0912561.1"
                     /translation="MTTVKVQKNQIIAKQKEKVKKWYIVQEGTVIQCNSYAKIVLGKN
                     AVIGISEGDRYLCDYVATEDSVLAVFNCESAEDLKEAIHGQESMRSILLRAALGQRQM
                     LLRTYAGFTNLVRQFHSFAENEYSQYENLCAQYHLDEQSFTRMDNFKPLEMVHRAENW
                     EIGNSASLTGSYLEEYVHLMQRDDNLCIGAIMEASYQTRRVTQGIIEMVEYLRYNQDI
                     LLAESKNDLFNLFFALAIQAQKKNYDVKP"
     gene            complement(781..2019)
                     /gene="coaBC"
                     /locus_tag="ACZO9R_05545"
     CDS             complement(781..2019)
                     /gene="coaBC"
                     /locus_tag="ACZO9R_05545"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR0912562.1"
                     /translation="MNKQSEKPRGLLSGKTVLLCVTGGIAAYKMPNVARMLKKLGCNV
                     HVLMTQNATNFITATTFETLTANKCLIDTFDRNFEFSVEHVALAKQADLVLIAPATAN
                     VIGKIANGIADDMLTTTVMACTCKCLIAPAMNHNMYHNPIVQDNIRKLEHYGYEMIEP
                     VRGMLANGDTGDGKLPSEEELAEYVLRELAAPKDMAGLKILVTAGPTQEAIDPVRYIT
                     NHSTGKMGYAIARRAMYRGADVTLVTGPTACEPPRFVNVVPVVTAAQMYDAVMSRAKE
                     QDIIVKAAAVADYRPASVAEEKIKKSDREASIELERTQDILAALGADKGKTILCGFSM
                     ETEHMLENSRKKLRKKNVDLIAANNLKVEGAGFGTDTNVLTLITEDGERELARMSKEE
                     AADELLTELLIISKGRAKGQ"
     gene            complement(2236..3249)
                     /locus_tag="ACZO9R_05550"
     CDS             complement(2236..3249)
                     /locus_tag="ACZO9R_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR0912563.1"
                     /translation="MQEGKKQRIEFLDYLKAVCVIMVIITHYGWEDKTSPFFTMLINM
                     AVPVFMIVSGYNFAMSNRKKADGNLEKMYGWNMMKPKLIRFLLPFFAICLLEILLLAA
                     QDKNIPLFRIFVLGAYGPGSYYVPIMLQLLVIFPLIYVMIAYNAKLGLAVAALANLAF
                     EVCVIVFDMDKYYYRLSIGRYLLLIAFGCYLYLHPEQRVKPYQMWMMFLAGLAYLIAV
                     FGFDKDFELFGYWKTTAMPVAFYIFPIVILLFRKFYHCTIPGFAGRLLTQIGKASYHI
                     FLIQMVYYHFELGGAIMQTAWYIAVPFNILVTVPLGLGFYELDNQFVKKLREVKHYIR
                     AVV"
BASE COUNT          834 a          977 c          659 g          932 t
ORIGIN      
        1 gcggctttac atcataattt ttcttctgcg cctgaatcgc cagagcaaag aacaggttaa
       61 aaagatcgtt tttcgattcc gccagaagaa tatcctgatt gtagcggaga tattccacca
      121 tctcaatgat tccctgcgtc acacgcctgg tctgataact ggcttccatg atcgccccga
      181 tgcagagatt atcgtcccgt tgcatcagat gcacatactc ctctaaataa cttccggtca
      241 ggctggcact gttgcctatc tcccagttct ccgcccggtg caccatctcc aacggcttga
      301 aattgtccat gcgcgtaaaa ctctgctcgt ccagatggta ctgtgcgcag agattctcat
      361 actggctgta ctcattctcg gcaaaggagt gaaactgcct taccagattg gtaaaaccgg
      421 cataggttct aagaagcatc tgccgctgtc cgagtgctgc ccgaagcaga atactgcgca
      481 tgctctcctg cccgtgaatc gcctccttta aatcctccgc cgattcacaa ttgaacaccg
      541 ccagcacgga atcctcggtc gcaacataat cacacagata tctgtcgccc tcagaaattc
      601 caatcaccgc attttttcca agaacaatct tggcgtagga attgcactga atgacagtac
      661 cctcctgcac gatataccat ttttttactt tctctttctg cttggcaatt atctggttct
      721 tctgtacctt tactgtcgtc atcgtctctc tgtcccctgc ctgcgcacat gaccgtgtgt
      781 ctactgcccc ttcgcgcgtc ctttacttat tattaacaat tccgtaagca gctcgtccgc
      841 tgcctcctct ttactcatcc gtgccagttc ccgctcgccg tcctctgtga tcagtgtcag
      901 cacgttcgta tcggttccga aaccggcgcc ttccaccttc agattattgg ctgcgatcag
      961 atccacattc tttttgcgaa gcttttttct tgaattctca agcatgtgct ccgtctccat
     1021 ggaaaacccg cacagtatcg tctttccttt gtccgccccc agagctgcca gtatatcctg
     1081 tgtgcgttcc agttcgatcg aagcttcccg atcggacttc ttgatcttct cctccgccac
     1141 agatgccggt ctgtaatccg ccaccgctgc cgccttgaca ataatatcct gctcctttgc
     1201 acggctcatg accgcgtcat acatctgtgc cgccgtcaca accggaacaa cattcacaaa
     1261 ccgcggcggc tcgcacgctg tcggtccggt cacaagtgtc acatccgccc ctcggtacat
     1321 ggcgcgtctc gcaatcgcat atcccatctt ccctgtggag tgattggtaa tatagcgcac
     1381 cgggtcaatc gcttcctgtg tcggacctgc agtgacaaga atttttaatc ctgccatgtc
     1441 ctttggcgca gcaagttctc ttaagacata ctcggccagc tcttcctcgg acggcagttt
     1501 gccgtctccc gtatcgccgt tcgccagcat cccccgcacc ggttcaatca tctcatagcc
     1561 gtaatgctcc agcttgcgga tattgtcctg cacaatcgga ttgtggtaca tattatggtt
     1621 cattgccggg gcgatcaggc acttgcaggt acacgccatc accgtcgttg tgagcatgtc
     1681 gtctgcaatt ccgttcgcaa tcttgccgat cacgtttgct gtagccggtg caatcagcac
     1741 cagatccgcc tgtttcgcaa gcgccacatg ctccaccgag aactcaaaat tccggtcgaa
     1801 cgtgtcaatc agacacttgt tcgccgtcag cgtctcaaac gtcgtcgctg tgataaaatt
     1861 cgtggcattc tgcgtcatca gaacatgtac gttgcaccca agcttcttca acatgcgcgc
     1921 tacattcggc atcttataag cggcaattcc tcccgtcaca caaaggagta cggttttccc
     1981 gctcaaaagt ccccttggct tttcgctctg cttattcatt ttttcctcct gctctcgttc
     2041 acttatccca tgatacgatg ctaaaattcc atttttattc tatactcttt tccctctctg
     2101 tgcaatgcct ttctgcctgc gcgcccaact ttcaccgcta aatgtaacgc ggcatttcct
     2161 gaaaaaagaa tcctattaca taaaaaacgc acaccgtgtc acgtgacaca acctgtgcgc
     2221 aaataaatat gttccttata caaccgcccg gatgtaatgc tttacctcgc gcagcttttt
     2281 cacaaactgg ttgtccagct catagaatcc aaggccaagc ggaaccgtaa ccagaatgtt
     2341 aaacgggaca gcaatatacc atgccgtctg catgatcgcg ccgcccagtt caaagtgata
     2401 gtataccatc tgaatcagga agatatgata ggaagctttt ccaatctgtg tgagcagtct
     2461 ccccgcaaat cccggaatcg tacagtgata aaatttccgg aacaataaaa taacgatcgg
     2521 gaaaatatag aacgcaaccg gcatcgctgt cgtcttccag tagccaaaca actcgaaatc
     2581 cttgtcgaat ccgaaaactg caatcagata tgccagtcct gccagaaaca tcatccacat
     2641 ctgatacggc tttaccctct gctccggatg cagatacaga taacagccaa acgcgattaa
     2701 gagcaggtat cttccgatgc tcaggcggta ataatattta tccatatcaa acacgatgac
     2761 gcacacctca aacgcgagat tggcgagcgc tgccacagca agtccaagct tagcattgta
     2821 tgcgatcatc acataaatca acggaaaaat aacaagcaac tgcagcatga tcggtacata
     2881 atagcttcca ggtccgtatg cgccaagcac aaaaatacgg aacaacggta tatttttatc
     2941 ctgcgctgcc agcagcagaa tctcaagcag acaaatggca aaaaacggca gcaaaaagcg
     3001 gatcagcttt ggtttcatca tattccagcc atacattttc tccagatttc cgtctgcttt
     3061 tttgcggttt gacattgcaa aattataacc gctcacaatc ataaataccg gcactgccat
     3121 gttgatcagc atcgtaaaaa aaggagatgt cttatcctcc catccatagt gcgtaatgat
     3181 taccataatg acacagactg ccttgagata atccagaaat tcgattctct gtttctttcc
     3241 ctcttgcatt tctaatcctc attcctcttt ttatacacaa tcctgatgtc ctctttttga
     3301 caccaattct gtatattacg cactttttgc atgattttca atagtaattg tggctaaatt
     3361 tgggcttcaa tcgagggatt tataaagcat ttctacgata tg
//
LOCUS       DBMDQJ010000215         3391 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10182_length_3391_cov_1.662006, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000215 DBMDQJ010000000
VERSION     DBMDQJ010000215.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10182_length_3391_cov_1.662006"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            14..997
                     /gene="pta"
                     /locus_tag="ACZO9R_05555"
     CDS             14..997
                     /gene="pta"
                     /locus_tag="ACZO9R_05555"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HIR0912564.1"
                     /translation="MFGFGQLIDILKKDPKKIVFTEGNDPRILEAASRLLAGTFLHPI
                     LVGNPDEIFKAAEESGFNIRGAEIIDPANYDRFDEMVDLFCELRKSKGVTPEQAKGIL
                     SQANYFGTMLVKMGVADALLGGATYSTAVTVRPALQLRKTKPGNSIVSSCFILVRPSA
                     TGENEVLAMGDCAINIHPTEDELVEIAGESAECAKIFGVDPKVAFLSYSTLGSGKGED
                     VDKMRNAAAKAKAKYPSLPIEGELQFDAAVSPRVARTKCPGNEVAGHANTFIFPDINA
                     GNIGYKIAQRLGNFEAYGPILLGLNAPINDLSRGCNASEVYSMAIITAALA"
     gene            1078..2415
                     /locus_tag="ACZO9R_05560"
     CDS             1078..2415
                     /locus_tag="ACZO9R_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912565.1"
                     /translation="MESLLFTREDLKKLIVPLIIEQALALSIGLFDTLMVSSCGEAAV
                     SGVSLVDSISTLLIQILSALATGGAVVCSQYIGKKMPERAKLSAGQLMFIMLMSAGTV
                     MVVILLSYRFLLRAIFGQIDADVMQNAEIYFIISAISYPFLGVYNAGAALFRAIGNSK
                     VSMYTSLVMNVINIGGNAVLIFGLKMGVLGAALATLTARIVSAIVMVVLLSRKDNPLC
                     IATPGCMKPQRDVIGKILKIGIPSGVENGMFQIGKLLVSSLTATFGTAAIAANAVANS
                     VAGFANIPGIAIGLAIVTVIGRCIGAGEKEQAKYYSKRLLGLAYAGMVLADLALFALV
                     KPIIACFALSAEAARIATQLLVSFAICAALIWPLSFTLPNVLRAAGDAKYTMEVSVFS
                     MWVFRVASSYFFAGTMGLGVLGVWIGMYVDWAFRTLLFVIRYRRGRWLEKRVV"
     gene            2534..>3391
                     /locus_tag="ACZO9R_05565"
     CDS             2534..>3391
                     /locus_tag="ACZO9R_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0912566.1"
                     /translation="MYKNMGKVKRTLEEYAILTVATLILVVGVYVFKFPNNFSFGGVT
                     GIAVVLSAVMPATPGNITFIINMALLVVGFIFLGKSFGIRTVYVSVLMSVGLSLAEVW
                     FPMEHPLTTQPVLELMYAIVLPAFSAAIMFNIGASGGGTDIIAMILKKYTKLNIGGAL
                     FLVDLGIVIASCFVFDAQTGLCSLCGLLAKSLVVDNVIESINLCKYFTIICNDPEPIC
                     DFITNELHRSATIYKAEGAYEHNQKTIIITIMKRSQAVELRNYIRIHQPTAFIAITNS
                     SEIIGKGFRG"
BASE COUNT          781 a          755 c          988 g          867 t
ORIGIN      
        1 gagagagtat actatgttcg gatttggtca gttgatcgat atcttgaaga aagatccaaa
       61 gaaaattgtt tttacagagg gaaatgaccc gcgtatttta gaggcagcat ccagattact
      121 ggcaggtacc ttcctgcacc cgattctggt tggtaacccg gatgagattt ttaaggctgc
      181 tgaggagagc ggattcaata ttcgtggcgc tgagatcatt gatccggcaa actatgacag
      241 atttgacgag atggtagatc tgttctgtga actcagaaag agcaaaggtg tgacaccgga
      301 gcaggcaaag ggcattctgt cccaggcgaa ctacttcgga accatgcttg taaagatggg
      361 cgttgcagat gcacttctcg gcggagcaac ctactccacc gcagttaccg tacgtcctgc
      421 attacagctg cgtaagacca agccgggcaa ctccatcgta tcttcctgct tcattctggt
      481 tcgtccgtct gcaaccggtg agaacgaggt tcttgcaatg ggtgactgtg caatcaacat
      541 ccatccgacc gaggatgagc tggttgagat cgcaggtgag tctgcagagt gtgcgaagat
      601 cttcggtgta gatccgaagg ttgcattctt aagctactct accttaggtt ccggcaaggg
      661 tgaggatgta gacaagatgc gcaatgcagc agcaaaggca aaggcaaaat atccgtctct
      721 tccgatcgag ggcgagttac agtttgatgc agcagtatcc ccgcgtgttg cacgtaccaa
      781 gtgcccgggc aacgaagtgg caggacatgc aaacaccttt attttcccgg atatcaacgc
      841 cggaaatatc ggttacaaga ttgcacagcg tcttggtaat ttcgaggcat atggtccgat
      901 cctgcttggc ttaaatgcgc cgatcaacga tctgtcccgc ggatgcaacg cttccgaggt
      961 atattccatg gcaatcatca cggctgcact ggcataatcc ggacactgca cagaacaata
     1021 gaatgacagt catttgtggc atggcgtgac gctgtgccac atttttttga gggaaaaatg
     1081 gaatcattac tttttaccag agaagatttg aaaaaactga ttgtaccgct cattattgag
     1141 caggcgctgg cattgagcat cggactgttt gacacgctga tggtctcctc gtgcggcgag
     1201 gcggcggtgt cgggcgtgtc gctggtggac agcatcagca cactgctgat ccagattttg
     1261 tcagcgcttg caaccggcgg cgctgtggta tgcagccagt atatcggcaa gaagatgccg
     1321 gaacgggcaa aattgtctgc cggacagctg atgttcatca tgctgatgtc cgcggggacg
     1381 gtgatggtgg tcatcctgct gtcctaccgc tttttgctgc gggcaatttt cgggcagatt
     1441 gacgcggacg tcatgcagaa tgcggagatt tattttatca tctccgccat atcctacccg
     1501 tttctgggcg tttacaacgc gggagcggcg ctgttccggg caatcggaaa cagtaaggtc
     1561 agcatgtata caagcctggt catgaatgtg atcaatatcg gcggcaatgc ggtgctgatc
     1621 ttcggactta agatgggcgt gctgggagcg gcgcttgcaa cgctcacggc gcggatcgta
     1681 tcggcgattg tcatggttgt gctgctctcg cggaaggaca acccgctgtg tattgcgacg
     1741 ccgggctgta tgaagccgca gcgggatgtg atcggaaaaa ttttaaagat cggcattccg
     1801 agcggtgtgg aaaacggaat gttccagatt ggaaagcttc tggtgtcgag tctgaccgcg
     1861 acgtttggca cggcggcgat cgcggcgaat gcggtggcga acagcgtggc gggatttgcc
     1921 aatattccgg gtattgcgat cggacttgcg atcgtgacgg tcatcggcag atgtatcgga
     1981 gccggggaaa aagaacaggc gaaatactac agcaagcgtc tgcttggtct ggcttatgcg
     2041 ggaatggtgc tggcggatct ggcactgttt gcactcgtaa aaccaatcat tgcctgcttc
     2101 gcgctatcgg cggaagcggc acggattgcc acacagcttc tggtaagttt cgcaatctgc
     2161 gcggcgctga tctggccgct cagttttacc cttccgaatg tgctgcgcgc cgcaggagat
     2221 gcaaagtata cgatggaggt cagcgttttt tccatgtggg ttttccgcgt ggcaagcagt
     2281 tacttttttg ccggaacgat gggacttggc gtgctcggtg tctggatcgg catgtatgtg
     2341 gactgggcgt tccgcacgct gcttttcgtg atccgctacc ggcgtggcag gtggctggaa
     2401 aagcgcgttg tatgacgccg caagggaatt gattcctaat attttcttat tttttgataa
     2461 tacagtttca tttttgcggg aaatacggta gaatatcaga gaaatacgtt cagatgatga
     2521 ggattttgaa gaaatgtaca agaatatggg aaaagtaaaa cgcacactgg aggagtatgc
     2581 cattctgacg gttgccacgc tgattctggt ggtgggtgtt tatgtgttca agttcccgaa
     2641 taatttttca tttggtggag tcacgggtat cgcggtggtc ctaagtgccg ttatgccggc
     2701 aactccggga aatatcacat ttatcatcaa tatggcgctg ctggtggtag gatttatttt
     2761 cctcgggaaa agttttggca tccgcaccgt gtatgtgagc gtgctgatgt ctgtggggct
     2821 aagccttgcg gaagtgtggt ttccgatgga gcatccgctg acgacgcagc cggtactgga
     2881 actgatgtat gcgattgtgc tgccggcgtt cagtgcggcg attatgttca atatcggagc
     2941 atccggaggc ggtacggata ttatcgccat gatccttaaa aagtatacga agctgaacat
     3001 cggaggcgcg ctgtttctgg tggatctggg gattgtcata gcttcgtgtt ttgtgtttga
     3061 tgcacagacg ggactttgtt cgctctgcgg acttctggcg aagtcgctcg ttgtggacaa
     3121 tgtcattgag agtatcaact tgtgcaagta ttttacgatc atctgcaatg atccggagcc
     3181 catctgtgat tttatcacga atgagctgca ccgcagtgcg acgatctata aagccgaggg
     3241 ggcatatgag cacaaccaga agacgatcat tatcactatt atgaagcgct cccaggcggt
     3301 agaactgcgt aattacatcc gcatccacca gccgaccgca tttattgcta tcacgaacag
     3361 cagtgagatc ataggcaagg gattccgcgg g
//
LOCUS       DBMDQJ010000216         3388 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10195_length_3388_cov_1.764831, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000216 DBMDQJ010000000
VERSION     DBMDQJ010000216.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10195_length_3388_cov_1.764831"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1348)
                     /locus_tag="ACZO9R_05570"
     CDS             complement(<1..1348)
                     /locus_tag="ACZO9R_05570"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912567.1"
                     /translation="MAVNSYREDEFMENTDKKKIFRRLFSYLLDYKAILIGVLLCMAV
                     TVAISLVNPLLIEEAIDHYIANKDMRGLMGLGLFALGLNIIFIIMVKIRMYVMAVVSN
                     KILLRIRQDLYEHIQTLSFSFFDSRPTGKILARIIGDVNSLRDVLVNVVTTLIPELIT
                     VIGVVVIMLIKDWRLALASLSTIPLMIGGIWFVQTASHKRWQIFRKKSSNLNAYVHED
                     IAGMNVVQSFGAEEETREIFGNLTDEHQQSFVDAVRYADMFGPVIDFCWGIGAMMLYL
                     VGVRFLGFEKVSVGLLVAFGTYINMFWNPIMNLSNFYNNLVTNLTAAERIFDILDTKA
                     DIVDAADVTELPEVQGRVEFSHVSFTYDKGTPAETKVLSDVSFVVEPGETIALVGPTG
                     AGKTTIVNLISRFYDIEDGVISVDGYDLTKISIESLRRQMGVMTQDNFIFHGTVREN"
     gene            complement(1538..3298)
                     /locus_tag="ACZO9R_05575"
     CDS             complement(1538..3298)
                     /locus_tag="ACZO9R_05575"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912568.1"
                     /translation="MKKLYTYIGRYWYGYLFAVFSMVTAIVLDMLYPKITQQIVDDVI
                     IGGKLELLTKLLVGIVIVGIGRSIFGYCKEFTFDVLASKIGSAMRKDLFDHIQSLSMG
                     YFEDTNTGELMARVKDDVDKIWNAMGYVGMLVIEVIIHVSMVLYCMFSLNWKLAFVPL
                     ATMILCGTVAIIMERKLDKIYEDISEENAVLTTVAEENLAGVRTVKAFAREKHEIEKF
                     LSHNNRYYELNVSQAKVLTKYYPLFSFVGKVLPVCSTILGGIFVMNGEMTLGALVAFV
                     EYSRNCTWPMEMLGWLTNDVSSAVASYKKIRKIYAEQPKIKNDEHPKVLDEIRGEICF
                     DHAGFVMDGQKILQDINVTVPAGKTLGIMGATGSGKSSIVNLLQRFYDVTDGAVKIDG
                     VDVRNMDVHQLRSNIAVVLQDVFLFSDTIEENIKMGQRKELQMEAVRLAAEDAKARGF
                     IEKMDEQYETVIGERGVGLSGGQKQRISIARALAKHSPILVLDDSTSALDMETEHEIQ
                     QTLNALRDTTKIIIAHRISAVRHADEIIYLADGKIAERGTHEELLAKKGLYYDTYMAQ
                     YGALAMSSASAGEPAPAACC"
BASE COUNT          819 a         1037 c          702 g          830 t
ORIGIN      
        1 tattttcacg cacagtgccg tggaaaataa aattgtcctg tgtcatgaca cccatctgcc
       61 gccgcaggct ttcgatggaa atcttcgtga gatcgtagcc atccaccgaa ataacaccgt
      121 cctcaatatc atagaatcgg ctgatcagat tcacgatcgt cgtcttgcct gcgcctgtcg
      181 gtccgacaag cgcaatcgtc tctcccggct ccaccacaaa actcacgtcg gaaagcacct
      241 ttgtctccgc cggcgttccc ttatcgtatg taaagctgac atgggaaaat tccactcttc
      301 cctgtacttc cggcagttcc gtaacgtctg ccgcatccac gatatctgcc tttgtatcca
      361 gaatatcaaa aattcgctcc gccgccgtta agtttgtcac cagattattg taaaaattac
      421 tcagattcat aatcgggttc cagaacatat tgatgtacgt gccgaatgca accagaagtc
      481 cgaccgaaac cttttcaaaa ccgagaaagc ggacgccgac cagatagagc atcatcgcgc
      541 cgattcccca gcagaaatca atcaccggtc cgaacatatc cgcatagcgc accgcgtcca
      601 cgaacgactg ctgatgctca tcggtcagat ttccgaaaat ctctcttgtc tcctcctcgg
      661 caccaaaact ctgcaccaca ttcattcccg caatgtcctc atgcacatag gcattgaggt
      721 tggatgattt tttgcggaaa atctgccagc gcttatgcga ggcagtctgc acaaaccaga
      781 tgccgccgat catcagcgga atcgtgctga gtgatgcaag tgccagacgc cagtccttta
      841 tcagcatgat caccacaacg ccgatgaccg taatcagttc cggaatcaat gttgtcacca
      901 catttaccag cacatcccgc agcgaattca catcaccgat gattctcgcc agaatctttc
      961 cggtcggtct gctgtcaaaa aaggaaaacg acagcgtctg aatgtgttcg tacagatcct
     1021 gacggatgcg cagcagaatc ttgttcgaca ccaccgccat cacatacata cggatcttca
     1081 ccataatgat aaaaataata ttcagtccca gcgcaaacag accgagtccc atcagccccc
     1141 tcatatcttt atttgcaata taatggtcga tcgcctcctc aatcagaagc ggattgacca
     1201 gtgaaatggc aaccgtcaca gccatgcaga gcaacacgcc gatcaagatt gctttataat
     1261 ccagtaagta ggagaacaac cttcggaaaa tcttcttttt gtcagtgttc tccataaatt
     1321 catcttcacg ataggaatta actgccatga actctcatcc ccttttcctc tatataaacc
     1381 tttccatccc tgccgttcgc ctgccctcag cagcacgctg ctggtgccgg ctctcctgca
     1441 ggggcgctta cagaagttct cattcgctgc gcccgctcgg gcggcttcgc ctccctcgct
     1501 gtacggcatt cgccgcatcc ctgccgttcg cctgctctca gcagcacgct gctggtgccg
     1561 gctctcctgc agaggcgctg ctcattgcta acgcaccata ctgtgccatg taggtgtcat
     1621 agtacaaacc tttctttgcg agaagctcct cgtgtgtccc gcgctccgca atctttccgt
     1681 ctgcaagata aataatctca tccgcatgac gcaccgccga gatgcggtgt gcaatgataa
     1741 tctttgtcgt atcgcgcaga gcgtttaacg tctgctggat ctcatgctcc gtctccatat
     1801 caagcgccga ggtcgagtcg tcaagcacca gaatcgggct gtgctttgcc agcgcgcgcg
     1861 caatgctgat ccgctgcttc tgtccgcccg aaagtccgac accgcgctcc ccgatgacag
     1921 tctcgtactg ttcatccatc ttctcaatga acccgcgtgc tttggcatcc tctgccgcga
     1981 ggcgcaccgc ctccatctga agttccttgc gctgtcccat cttgatgttc tcctcgatcg
     2041 tatccgagaa aaggaacaca tcctgcaaaa caaccgcgat gttgctgcgg agctgatgca
     2101 catccatatt tcgcacatcc acaccgtcaa tcttcaccgc gccgtccgtc acatcgtaaa
     2161 accgctggag cagattgacg atggaggatt tgccggaacc ggtcgccccc atgataccga
     2221 gcgtctttcc tgccggaacg gtaacattga tgtcctgcag aatcttctgc ccgtccatga
     2281 caaagcccgc gtggtcaaaa cagatctctc cccggatttc gtcaagcacc tttggatgct
     2341 catcgttttt gatcttcggc tgctccgcat aaatcttgcg gatcttctta taagatgcca
     2401 ccgcggacga cacatcgttc gtcagccagc ccagcatctc catcggccag gtacagttcc
     2461 tgctgtactc cacaaacgcc accagcgcac ccagtgtcat ctcaccgttc atgacaaaaa
     2521 taccgcccag gattgtggaa caaaccggaa gcacctttcc cacaaacgaa aacagcgggt
     2581 aatactttgt cagtaccttt gcctgtgaaa cattcagttc gtaataccgg ttgttgtggg
     2641 ataaaaattt ttcaatctca tgcttttccc ttgcaaacgc cttgactgtg cgcacacctg
     2701 caagattctc ctccgccacg gttgtcagca cggcattttc ctcactgatg tcctcgtaaa
     2761 tcttgtcgag cttgcgctcc atgatgatcg cgaccgtacc gcacaggatc attgtggcaa
     2821 gcggcacgaa cgccagcttc cagttgagcg aaaacatgca gtacagtacc atggacacat
     2881 gaatgataac ctcgatgaca agcatgccca catagcccat cgcattccag attttatcca
     2941 cgtcatcctt gacacgcgcc atcagttctc ccgtgttcgt gtcctcaaaa tatcccatgg
     3001 acagcgactg gatatggtcg aacagatcct tgcgcattgc cgatccgatc ttggatgcca
     3061 gcacgtcgaa cgtgaactcc ttgcagtagc cgaaaatgct cctgccgatt cccacgatca
     3121 cgattccaac cagaagtttc gtcaaaagct ccagttttcc gcctatgatg acatcatcca
     3181 caatctgctg tgtgatcttc ggatacagca tatccagaac gatcgccgtc accatcgaaa
     3241 ataccgcaaa cagataccca taccaatagc gtccgatata ggtgtataac tttttcattc
     3301 cctaatctcc tccttcttgt cagttccccg gtgtcacctt cctgtaaaac aaaaaagcga
     3361 ggggacatcc tgttacggat tctcctcg
//
LOCUS       DBMDQJ010000217         3382 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10219_length_3382_cov_1.312100, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000217 DBMDQJ010000000
VERSION     DBMDQJ010000217.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10219_length_3382_cov_1.312100"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(26..718)
                     /locus_tag="ACZO9R_05580"
     CDS             complement(26..718)
                     /locus_tag="ACZO9R_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HIR0912569.1"
                     /translation="MKEFLNQSVFFGVAISVLTYELGVFLKKKLRWPICNPLLISIVA
                     VIVFLAAFDIPYESYNAGAKYLSYLLTPATVCLAIPLYEQVELLKKHSKAIVLGLISG
                     VITSLVCVLVLALLFHFDHAQYVTLLPKSITTAIGMGISEELGGYVTITVAVIIITGV
                     LGNMLAETVCRIFKIEEPVAKGIAIGASSHAVGTVKAMEMGEIEGAMSSLAIAVAGLL
                     TVVLAPVFAGLM"
     gene            complement(723..1076)
                     /locus_tag="ACZO9R_05585"
     CDS             complement(723..1076)
                     /locus_tag="ACZO9R_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HIR0912570.1"
                     /translation="MKHLRQFLIILLFSFLGEGLKALLPLPVPASIYGLVLLFTALEL
                     GIIKLSAVENAGKFLIEIMPVMFIPAGTGLVESWSALKPICVQVVVIMFVSTIVVMVI
                     SGRVTQFVIRRNRKA"
     gene            complement(1185..3158)
                     /locus_tag="ACZO9R_05590"
     CDS             complement(1185..3158)
                     /locus_tag="ACZO9R_05590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR0912571.1"
                     /translation="MIIDRRLNQKGTDENIGTSRVNLTQRYVESVKYNGLGDFTEELR
                     EEADYRKRIEKIFSEIHMTGETYADLQPEALMEAYWQGEEMISRYYWAKRRDTDDIIW
                     VRVDVRIVPQLDTGDLFAFYNNWDVTHEKNRDRMMQLIIEFDYDYVEYICLQNGHFEI
                     MAQEKSSMCPSARGTDYDADIRDYLTRVAVTDQLEAHIRAMQTEEIRRNLEAEPLYIQ
                     EIDVRESDGSVRRKMIRYTYMDKQMETVFKSCVDIEDIVTEEKKKQERLERAIEETER
                     ANCAKSEFLAHMSHDIRTPMNAIIGMTSVARQECQDETICGYLDKIKSSSEFLLGLIN
                     DMLDISRIESGNLNLKSEQMSREMFGTAINTTIGPLMEQKNITFQCDLQHMEEMIYTD
                     AVRFKQIFFNLLSNAAKYTPEGGNVWLLTERLRTEGNDEWVRFTVRDSGIGMSEEFLE
                     HAFEPFSQEGNRTLALQWQGTGLGLAIVKKLVVMMHGTIEIHSKLGEGTGVILDLPLT
                     ISGVDAPEKMPQAVMAKQNLEGRRVLLAEDNEINTFVARRILESKGIIVEHAENGKRA
                     VEMVEQNPEEYFDAIVMDIRMPVMSGLEAARQIRAMGRTDAQTVPIIAMTANAYEEDV
                     SQSLAAGMNAHLAKPIEPQVILDTLAGYIGSKN"
     gene            complement(3191..>3382)
                     /locus_tag="ACZO9R_05595"
     CDS             complement(3191..>3382)
                     /locus_tag="ACZO9R_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR0912572.1"
                     /translation="LLDWHGLRTQLEKQMKDGIGEQEAKTINMFLLNLFFIKPYEPEQ
                     DFYEQYEERRAMAGQAGLK"
BASE COUNT          817 a         1007 c          631 g          927 t
ORIGIN      
        1 gcattttggg caggcagacc gcatgctaca taagtcctgc aaataccggt gccagcacaa
       61 ccgtcagaag tcccgcaacc gcaatggcaa gactgctcat cgcgccctcg atctctccca
      121 tctccatcgc cttgaccgta ccaaccgcat gggatgacgc tccgattgca attcccttcg
      181 ccaccggctc ctcaatctta aatatacggc agaccgtctc cgccagcata ttgccaagca
      241 caccggtaat aataatcaca gcaaccgtga tggtcacata accgcctagc tcctccgata
      301 ttcccatgcc gatcgcggtc gtgatggatt tcggcagaag cgtcacatac tgcgcatggt
      361 caaaatgaaa caacagtgcc agtacaagca cacacacaag gctcgtaatc acaccggaga
      421 tcagtccaag cacgatcgcc ttggaatgtt ttttcaaaag ttccacctgc tcgtagagtg
      481 ggatcgccag gcagaccgtt gccggcgtca gaagatagct cagatatttt gctcccgcgt
      541 tataactctc gtaaggaata tcaaacgcgg ccagaaaaac aatcaccgca acgatcgaaa
      601 ttaaaagtgg attacatatc ggccatctca gcttcttttt taaaaataca cccagttcat
      661 aggtcaatac gctgattgct acgccaaaaa atacagactg atttaaaaat tccttcattg
      721 ccttatgcct ttctatttct gcgaattaca aactgtgtca ctcttcccga aatcaccata
      781 accacaatcg tggatacaaa catgatcaca accacctgca cacagatcgg ctttaaggcg
      841 ctccaggact cgaccagtcc cgtccccgcc ggaataaaca tcaccggcat gatctctatc
      901 aaaaatttcc ctgcattttc cactgcagaa agttttatta ttccaagctc cagtgctgta
      961 aacaacagta ccagtccata gatgcttgcc ggtaccggaa gcggaagtaa tgcctttagc
     1021 ccttctccca gaaatgaaaa aagtaaaata atcaaaaatt gtctcaaatg cttcatcctg
     1081 ccgtctcctc tcatcggaac ttattatagc aacagtatca caacttttac atcgacattc
     1141 cgacgaaatg catgcccttc tttttccatt tacagtgcaa ctttctagtt cttactgcct
     1201 atgtaccccg cgagtgtatc cagaatcacc tgcggttcga tcggttttgc aagatgcgcg
     1261 ttcatcccgg ccgccaacga ctggctgaca tcttcttcat aggcattggc cgtcatggca
     1321 attataggca cagtctgtgc atccgtccgg cccattgccc gaatctgtct ggctgcctcc
     1381 agtccgctca tcaccggcat ccggatatcc atcacgattg cgtcaaaata ttcctccgga
     1441 ttctgctcca ccatttccac cgctcttttt ccattttccg catgttccac aataattccc
     1501 tttgattcca gaatacgtct ggccacaaaa gtattgatct cgttgtcctc tgcaagaagc
     1561 acccgtctgc cttccaggtt ctgttttgcc atgactgcct gtggcatttt ttccggagca
     1621 tcgacgcccg agatcgtaag cggcagatca aggattaccc cggtaccctc tcctaattta
     1681 ctgtgaatct caatcgtacc gtgcatcatg accaccagtt tctttacgat cgcaagtcca
     1741 agaccggttc cctgccattg cagggcaagc gtacggttcc cctcctgcga aaacggttca
     1801 aacgcatgct ccagaaattc ctcactcata ccgattccgc tatcccgcac cgtaaagcga
     1861 acccattcat cgtttccttc cgtgcggagc ctttcggtca gaagccacac atttccgccc
     1921 tccggcgtgt attttgctgc attggacagc agattgaaaa aaatctgctt gaaccgcacc
     1981 gcatcagtgt atatcatttc ttccatatgc tgcagatcac actggaacgt aatgtttttc
     2041 tgctccatca gcgggccaat ggtggtattg attgccgttc caaacatctc tctgctcatc
     2101 tgctccgact tcaggttcag attgccgctc tctatgcgtg aaatatccag catatcattg
     2161 attagtccga gcagaaactc acttgaggat ttaatcttat ccagatatcc gcagatcgtc
     2221 tcatcctgac attcctgcct tgcaacggat gtcatgccga taatcgcatt catcggcgtc
     2281 cggatatcat gactcatatg ggccaggaat tcactcttgg cgcaattcgc ccgctccgtt
     2341 tcctcaatcg cccgctccag acgttcctgc tttttcttct cctccgttac gatgtcttcg
     2401 atgtccacac agcttttgaa taccgtctcc atctgcttat ccatataggt ataacggatc
     2461 attttacgcc gcactgaacc gtcagactct ctcacatcaa tttcctgtat gtaaagcggc
     2521 tccgcctcaa gattacgccg gatttcctct gtctgcattg cccggatgtg tgcttccagc
     2581 tgatccgtca ctgcgactct ggtaagataa tcacggatat ccgcgtcgta gtctgttccc
     2641 ctggcgctcg gacacatgga gcttttttcc tgcgccataa tctcgaaatg gccattctgc
     2701 agacagatgt attccacata atcatagtca aactcaatga tcagctgcat catacggtca
     2761 cggtttttct catgcgtgac atcccagtta ttgtaaaagg caaacaaatc tcccgtatcc
     2821 agctgcggta caatcctcac atccacacgc acccagataa tgtcgtccgt gtcccgcctc
     2881 tttgcccagt agtaacgcga tatcatttcc tctccctgcc agtaggcttc catcaatgcc
     2941 tctggctgta gatccgcata cgtctcccca gtcatatgaa tttccgagaa aatcttttca
     3001 atgcgcttcc ggtagtctgc ttcctcacgc aattcttcgg taaaatcgcc cagaccattg
     3061 tatttgacgg attccacata acgctgcgtg agattgacgc ggctggttcc aatattctca
     3121 tctgtccctt tctgattcaa tcttctgtcg attatcatac ttccatctcc tgccatacat
     3181 tatccgcagt ttattttaac cctgcctgac cggccatcgc tcttctctcc tcatactgtt
     3241 catagaagtc ctgctccggt tcatatggtt ttataaaaaa cagatttaac aaaaacatgt
     3301 tgatcgtctt tgcctcctgt tcaccaattc cgtctttcat ctgtttctcc agctgcgtcc
     3361 gcaggccatg ccagtccagc ag
//
LOCUS       DBMDQJ010000218         3371 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10257_length_3371_cov_1.237309, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000218 DBMDQJ010000000
VERSION     DBMDQJ010000218.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3371)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3371)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3371
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10257_length_3371_cov_1.237309"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2127)
                     /locus_tag="ACZO9R_05600"
     CDS             complement(<1..2127)
                     /locus_tag="ACZO9R_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR0912573.1"
                     /translation="MTREEFATLVGSRPVILDGATGTNLQKAGMPVGVCPEQWILENP
                     GVLIELQERYVEAGTDILFAPTFTASRIKLEEYGLEDSLVQMNRELVALSKRAAGGRA
                     YVAGDLTMTGRQLYPLGDLMFEDLVEVYKEQAKVICEAGADLFVVETMMSLQECRAAV
                     IAIREVCDLPIMVSLTYNEDGRTLYGTDPATAVIVLQSLGADAVGLNCSTGPEAMIDP
                     VQQMAEYATIPLLAKPNAGMPELCDGVTVYRTTPEEFASVGAKLVEAGAAIIGGCCGT
                     TPEHICALKQAVGSMPVHMPLTKKRRMLASERMHVEIRLDGKFMVIGERINPTGKKKL
                     QAELKEGSLSMVRTMATEQEENGAQILDINMGMNGIDEKQMMLDAIYEVTSTVDLPLC
                     IDSSHVDIIEAALRIYPGRALVNSISLEKEKIEYLLPIAKKYGAMFILLPLSDEGLPK
                     DSAEKHGIIREILRRAEAIGMGKEDIVVDGLVATIGANPKAALECFETFSFCKNEMEL
                     PTVCGLSNISFGLPERSYVNTAFLTMAIGNGLTMAIANPSQELLMNAAFASDMLLNKE
                     ESGIRYIGRMNYLSEKHEGMEHVWVPVGTAKGAAVKGTAAGQTGNAGGTKDSGNANEA
                     RSAVFTAVLKGNKNHILDEVKHALDTGEKPDDIINGHLIPAINEVGELFDKQKYFLPQ
                     LISSANTMKVAIEYLEPMLARSDKEPK"
     gene            2309..3151
                     /locus_tag="ACZO9R_05605"
     CDS             2309..3151
                     /locus_tag="ACZO9R_05605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR0912574.1"
                     /translation="MKQPVSLIALDMDGTLFNGQSEISKEDQDAIREASSRGIHVAIS
                     TGRPYAGLPVTLLSGLGVSYAITSNGAAVYHLPDRLCIHESAMEPALVCPIIEELQKK
                     SIHMDAFINGDGFSQRSCEGKIDCLDMPTSIKRYIHRTRTFTDDLAAYIRVHNLNVQK
                     MTLNFYPQEDGTFLYRDEVMQILSEHPEITFLSGGYHNLEFTRAGVTKGNGLTLLCSH
                     LGVDIEETLACGDTQNDIDILKTAAIGVAMENALPEVKEIADFVTRSNENSGVAHAIH
                     TFAL"
     gene            complement(3161..>3371)
                     /locus_tag="ACZO9R_05610"
     CDS             complement(3161..>3371)
                     /locus_tag="ACZO9R_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HIR0912575.1"
                     /translation="MALSDIEEPVRLKAKIRYSHAGSCCTVRRTGADEICCEFEEPVR
                     AVTPGQAVVLYDGDYVAGGGVITHA"
BASE COUNT          716 a         1099 c          767 g          789 t
ORIGIN      
        1 cttcggctcc ttgtccgagc gtgctaacat cggctctaaa tattcgatcg ccaccttcat
       61 cgtattcgca gaagaaatca gctgcggcag aaaatacttc tgcttgtcga acagctcgcc
      121 gacctcgttg atcgccggga tcagatgtcc gttgatgatg tcatccggct tctccccggt
      181 gtctaacgca tgtttgacct cgtctaaaat atgattctta ttgcccttta agaccgccgt
      241 aaaaaccgca ctgcgcgcct cattcgcgtt cccggaatct tttgtgccac ctgcgtttcc
      301 cgtctgtccc gcggcagtac ctttcacggc agcgcccttc gccgtcccga caggtaccca
      361 cacatgttcc atgccctcat gcttctcgga cagataattc atgcgcccga tatagcggat
      421 gccgctctcc tccttgttca gaagcatgtc cgatgcaaat gccgcattca tcagaagttc
      481 ctgggaagga tttgcaatcg ccatcgtaag gccatttccg atcgccattg tgagaaatgc
      541 cgtgttgaca taggagcgct ccggaagtcc gaacgaaatg ttggacagtc cgcagaccgt
      601 cggcagttcc atctcatttt tgcagaaaga aaatgtctca aaacactcca gcgccgcctt
      661 cggattggca ccgatcgtcg ccaccaatcc gtccaccaca atgtcctctt ttcccatgcc
      721 gatcgcttcc gcgcgtctta agatctcacg gatgatcccg tgcttttccg cagaatcctt
      781 cggcagcccc tcgtccgaga gcggcagcaa gataaacatg gcaccgtact tcttcgcgat
      841 cggcagcaga tactcgatct tttctttttc cagagaaatg gaattgacga gcgcacgacc
      901 cggatagatg cgcagcgccg cctcgatgat atccacatgg ctggaatcga tgcagagcgg
      961 cagatctacc gtggaagtca cctcatagat ggcgtctaac atcatctgct tctcgtcaat
     1021 tccattcatt cccatattga tgtccagaat ctgtgcgccg ttctcctcct gctccgtcgc
     1081 catagtgcgc accatactga ggcttccctc ttttaactcc gcctgcagct ttttctttcc
     1141 tgtcggattg atccgctctc cgatcaccat gaacttaccg tcaagccgga tctccacatg
     1201 catccgctcc gaggcgagca tacgtctttt ctttgtgaga ggcatatgca ccggcatgga
     1261 acccaccgcc tgctttaatg cgcagatatg ctccggtgtc gtgccgcagc atccgccgat
     1321 gatcgccgcg cccgcctcca caagcttcgc gccgactgac gcaaactcct ccggcgtggt
     1381 acggtatacg gtcacgccgt cacagagctc cggcataccg gcattcggct ttgccagaag
     1441 cggaatcgtc gcatactccg ccatctgctg taccggatca atcatcgcct ccggtccggt
     1501 ggagcaattc agtcccacag catccgctcc gaggctctgc agcacaatca ccgcagtcgc
     1561 cggatcggtt ccgtagagcg tccgcccatc ctcattgtag gtcagcgaca ccataatcgg
     1621 gagatcacac acttcgcgga tagcaatgac cgcagcacgg cattcctgaa gactcatcat
     1681 ggtctccacg acaaaaagat ccgctcccgc ctcgcagatc accttcgcct gctccttgta
     1741 gacttctacc agatcctcaa acatcagatc gccgagcgga taaagctgcc gtcccgtcat
     1801 cgtcagatcg cccgccacat atgccctgcc gcctgccgcg cgctttgaga gcgccaccag
     1861 ctcacggttc atctgcacca gggaatcctc cagtccgtac tcctccagct tgatccggct
     1921 tgccgtaaag gtgggcgcga acaaaatatc ggtgcccgcc tccacatagc gctcctgcaa
     1981 ttctatcaga acccccgggt tctccagaat ccactgctcc gggcagaccc cgaccggcat
     2041 tcctgctttc tgcagattcg ttcctgtcgc gccgtccaat atcaccggtc tgcttcccac
     2101 aagtgttgca aattcctctc tggtcattcc cgtatcctct ctatcatccg ttctcgctta
     2161 tcaagccaca tcttaccatt tccccatgcc gggtgcaagt attttccaat gtttcctgca
     2221 atccgcgcca cggcaccgcc gtttcttgat tgttttccca ttttattgta tcattctgta
     2281 gatacattta cagaaaggat actcgactat gaaacagcct gtcagtctga ttgccctcga
     2341 tatggacggc actcttttta acggacaaag tgagatctca aaggaggatc aggacgccat
     2401 tcgcgaagct tcctcccgcg gaattcacgt tgccatatcg accggacgcc cttacgccgg
     2461 gcttcccgtc acacttttat ccggtctcgg cgtctcctat gccatcacct ccaacggtgc
     2521 tgccgtctat catctgccgg accgcctctg catccatgaa tctgcgatgg agcctgcgct
     2581 tgtatgcccc atcatcgaag aactgcaaaa aaagagcatc catatggatg cttttatcaa
     2641 tggcgacggc ttcagccagc gttcctgtga gggaaagatc gactgcctgg acatgcccac
     2701 atcaatcaaa cgctacattc accggaccag aaccttcacc gatgatctcg ctgcctatat
     2761 ccgcgtccac aatctgaacg tccagaagat gacgctgaat ttttatccgc aggaggacgg
     2821 cacttttctc taccgcgacg aagtgatgca gatcctgtca gagcaccccg agatcacctt
     2881 tctctccggc ggctaccaca atctggaatt caccagagcg ggcgtcacca aaggaaacgg
     2941 tctcaccctg ctctgttcgc acctcggcgt cgatatcgaa gagacgcttg cctgcggcga
     3001 cacccagaac gacatcgaca ttttaaaaac cgcagcgatc ggcgtcgcca tggaaaatgc
     3061 cctgccggaa gtgaaggaga tcgcagactt tgtgacgcgc tccaatgaaa acagcggcgt
     3121 cgcgcatgca atccacacgt ttgccctgtg atgcaggcgc ttaagcgtgt gtgatcacgc
     3181 cgcctcccgc gacatagtct ccgtcgtaca gcaccactgc ctgtcccggt gtcacggcgc
     3241 gcaccggctc ttcaaattca cagcagatct catccgcacc ggtgcgccgc acggtgcagc
     3301 agcttcccgc atgcgagtac cggatctttg ccttcaggcg aaccggctcc tcaatgtcgg
     3361 agagcgccat a
//
LOCUS       DBMDQJ010000219         3369 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10264_length_3369_cov_1.580783, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000219 DBMDQJ010000000
VERSION     DBMDQJ010000219.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10264_length_3369_cov_1.580783"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="ACZO9R_05615"
     CDS             complement(<1..937)
                     /locus_tag="ACZO9R_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR0912576.1"
                     /translation="MTEMLWAVVLLVVGFVLLIKGADFFVEGSSSVAKMLKVPSIIIG
                     LTIVAMGTSLPECAVSITASVTNNNALAVSNAIGSNIFNLMVVCGFCALFNPLYVEKS
                     TLKKEFPFSVVCAVLLLVCGYLGMQLGRADGIILLIVFGAFLVWMVRSALKARAAAGD
                     DEYEVLPVWKCIVFIVGGIVAIKFGGDFVVNGASTVATKLGLSQNLIGLTIVACGTSL
                     PELVTSVVAARKNELDMALGNVIGSNIFNILFVLGIAATISPITFIMENVIDIVILVV
                     MSVLVWAFAWTKKKLDRFEGIVMLALYAVYLVYICM"
     gene            complement(1015..1596)
                     /locus_tag="ACZO9R_05620"
     CDS             complement(1015..1596)
                     /locus_tag="ACZO9R_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR0912577.1"
                     /translation="MAVFIELLLIGVGLSMDAFAVSICKGLAMRRVNKKQAFVIGLFF
                     GGFQALMPFIGWALGTQFESYITSIDHWIAFVLLVFIGGKMIAEAVRPEDENVEIDKL
                     DPPLDLKEMLVLAVATSIDALAVGITFAFLNYPIGEAVTIIGLTTFCISIGGVYVGNF
                     FGNRYKKKAELAGGIILVLIGVKILLEHLGFVG"
     gene            complement(1929..>3369)
                     /locus_tag="ACZO9R_05625"
     CDS             complement(1929..>3369)
                     /locus_tag="ACZO9R_05625"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR0912578.1"
                     /translation="DSDYEVPDSLRSIMRGYQKSGFLWLKTLRENGFGGILADDMGLG
                     KTLQVISLLLSEQESAKAGEREWHRSLIVCPASLVYNWQKEISRFAPQIKTTLVTGLA
                     TERQRIVRHTKEGEVLITSYDLLKRDVELYRDMVFAIQVIDEAQYIKNPGTQAAKGVK
                     QITAGFKLALTGTPIENRLSELWSIFDYLMPGFLYTYQRFREEIEIPVVVNGDENRMQ
                     RLQRMIRPFILRRLKGEVLRDLPAKLEENVFAKLEGEQLALYDAHVQRMKESLEGKSE
                     KEFRSEKIQILAELTRLRQICCDPGLLFEGYKGESAKAQMCMELIENAVGAGHKVLLF
                     SQFTSMLERLAAGLKKAGIDYYMLTGSVGKEKRMQMVESFNEDDVPVFCISLKAGGTG
                     LNLTAADIVIHYDPWWNVAVQNQATDRAHRIGQKHVVTVYKLVSEGTIEEKIIAIQER
                     KKELAKQVLEGEGMDSVSFTKEEILELLG"
BASE COUNT          847 a         1040 c          692 g          790 t
ORIGIN      
        1 gcatgcagat atacacaaga tagaccgcat agagcgccag catcacaatg ccctcgaagc
       61 ggtccagctt cttcttcgtc caggcgaacg cccacaccag tacgctcatc acaaccagaa
      121 tcacaatatc gatcacattt tccatgataa atgtgatcgg actgatcgtt gctgcgatac
      181 cgagcacaaa cagaatattg aaaatattgg agccgatcac attgccgagc gccatatcca
      241 gttcattttt gcgcgctgca accactgagg tcacaagttc tggcaggctg gttccacagg
      301 caacaatcgt aagtccgatc agattctggc tgagtcccag cttcgtggca accgtggatg
      361 cgccgtttac aacaaaatcc ccaccgaact tgatcgccac aataccgccg acgataaaca
      421 caatgcattt ccacaccggc aatacctcat actcatcatc tccggcagca gcacgcgctt
      481 tcagtgccga gcgcaccatc cagaccagaa aagcgccaaa tacaataaga aggatgattc
      541 cgtctgccct gccaagctgc attcccagat atccgcagac taaaagcaag actgcacaga
      601 ctacagaaaa cggaaattct ttttttaacg tactcttttc cacatagagt ggattaaaaa
      661 gggcacagaa tccacagacc accatcagat taaaaatatt agagccaata gcattgctga
      721 ctgcaagtgc attattgttc gttaccgatg ccgtaatact gaccgcacat tccggaaggc
      781 ttgtccccat cgccacgatc gtaagtccta taatgattga gggcactttc agcatctttg
      841 ccacggaaga gcttccctcc acaaaaaaat ctgctccctt aatcagaagc acaaatccaa
      901 caacaagcag cacaactgcc caaagcattt ctgtcataat aatctccatt cctctctgtt
      961 ttctctactg taattatttt ctcaaaatta tctcaacttc cggtagtccg gcacctatcc
     1021 cacaaacccg agatgctcca gcagaatctt cacgccgatc agcaccagga taatacctcc
     1081 agcaagttct gcctttttct tatagcggtt tccgaaaaaa ttgcctacat agacgccgcc
     1141 gatggagata caaaacgtgg tgagaccgat gatcgtaacc gcctccccga tcgggtaatt
     1201 caaaaatgcg aatgtaattc cgaccgcaag tgcatcgata ctggtcgcca ccgccagcac
     1261 aagcatctct tttaagtcga gtggcggatc cagcttgtcg atctccacat tctcatcctc
     1321 cggacgcacc gcctcggcga tcatcttgcc gccgatgaat accagcagca caaaggcaat
     1381 ccagtgatca atactggtaa tgtagctctc aaactgtgtg ccgagcgccc agccgataaa
     1441 cggcatcagc gcctgaaatc ctccaaaaaa cagaccgatc acaaacgcct gttttttgtt
     1501 taccctgcgc atcgcaagac ctttacagat cgacactgca aacgcgtcca tcgaaaggcc
     1561 cactccaatg agcagcaatt caataaaaac agccatacca acctccacat tttattctgc
     1621 ccctgccaca gggcgcacat acacattcag cccggatgat gcattttccg ccggcgcaca
     1681 cataaaaaag agactgatgt atatcctctg cgcaccgctc ccgtctccgt ctgcgatata
     1741 acaccaaaga tatacatgag tctcattctt taagatttgc caggtcaggc tgtccgaccg
     1801 gtctgttgac aaatcacgct ctgcgcgaac tactccctca cggaatctgc attcatcaat
     1861 aatgctgttt ccttactata tagcagccca cattttttgt caactgattt gtcgaaaaaa
     1921 caaccttttt atcccagaag ttcgagaatt tcctccttgg taaaggaaac agagtccatt
     1981 ccctccccct cgagcacctg ttttgccagt tcctttttgc gctcctggat cgcaatgatc
     2041 ttctcctcga tcgttccctc ggataccagc ttgtagaccg tcacgacatg cttctgcccg
     2101 atgcggtggg cgcggtccgt cgcctggttc tgcaccgcca cgttccacca cgggtcatag
     2161 tggatcacaa tatccgcggc cgtcaggttc agcccggttc ctcctgcctt taaggaaata
     2221 cagaataccg gcacgtcgtc ctcgttaaag ctctctacca tctgcatgcg cttttccttg
     2281 ccgacagaac cggtcagcat atagtagtca atcccggcct tcttaagccc tgccgccaga
     2341 cgttccagca tggacgtaaa ctgcgagaac aataacacct tgtgcccggc gccaaccgcg
     2401 ttctcgatca gttccatgca catctgcgcc tttgcggact cccccttata gccctcaaac
     2461 agaagccccg ggtcacagca gatctggcgc agtctcgtca gctccgcaag aatctggatc
     2521 ttctcggagc ggaattcctt ctcggatttg ccctccaggc tctccttcat gcgctgcaca
     2581 tgggcgtcat agagcgcaag ctgctctcct tcgagcttcg cgaagacatt ctcctccagc
     2641 ttcgccggaa gatcccgcag cacttccccc ttcagacggc gcaggataaa cggccggatc
     2701 atgcgctgca ggcgctgcat acggttttca tccccattta ccacgaccgg gatctcgatc
     2761 tcctcccgga accgctgata agtatataaa aatcccggca tcagataatc aaaaatactc
     2821 cacagttcac tcagccggtt ctcaatcggc gttccggtca gcgccagctt gaaacccgcg
     2881 gtaatctgct tgacgccttt tgccgcctgg gttccagggt tcttgatata ctgcgcctcg
     2941 tcgattacct gaatcgcaaa caccatatcg cggtacaact ccacgtcgcg ttttaacaga
     3001 tcgtaggacg taatcagcac ttccccttcc ttcgtgtgtc tgacaatcct ctggcgctct
     3061 gtggcgagtc cggtcacgag cgtcgtcttg atctgcggtg caaaacggct gatctctttc
     3121 tgccagttgt agacgagcga cgccggacat acgataaggg aacggtgcca ctcccgctct
     3181 cctgccttgg cactctcctg ttccgacaag agcagactga tgacctgcag ggtcttgccc
     3241 agtcccatat cgtccgcaag gattccgccg aatccgttct cgcgcagtgt ctttaaccac
     3301 agaaaaccgc tcttctggta gccgcgcatg atgctgcgga gcgaatccgg cacctcataa
     3361 tcgctgtcc
//
LOCUS       DBMDQJ010000220         3368 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10269_length_3368_cov_1.859810, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000220 DBMDQJ010000000
VERSION     DBMDQJ010000220.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10269_length_3368_cov_1.859810"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="ACZO9R_05630"
     CDS             <1..243
                     /locus_tag="ACZO9R_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912579.1"
                     /translation="SCFREEVRFQGERRSIRQRSGDSLDGPINLQLEMLGEKYGTETT
                     GTEYGKNPVLAEQNNFFYAKSAPFIVWQIRISCICL"
     assembly_gap    260..269
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            338..1117
                     /locus_tag="ACZO9R_05635"
     CDS             338..1117
                     /locus_tag="ACZO9R_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HIR0912580.1"
                     /translation="MKIEKVNDNQIRCTLTREDLAERQIKLSELAYGTEKAKLLFRDM
                     MQQAAYEFGFEADDIPLMIEAIPLSQDTIILVITKVEYPEELDTRFSRFSDPDPEDVY
                     EEAMAGGGAAPAPEGADDILGLFRRIQEERRKAADAQGSKDSFVSEENDKKKETPVNV
                     LVDITKLFVFSDFSEITRLAHTLQGFYQGNNDLYKNEAAHKYYLLVSKSRQTPEEFNK
                     VCNILSEYGTQQTYTPAAGAFMKEHYQVILKNHALQTLAEL"
     gene            complement(1207..1416)
                     /locus_tag="ACZO9R_05640"
     CDS             complement(1207..1416)
                     /locus_tag="ACZO9R_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HIR0912581.1"
                     /translation="MTQITKSTMIGELLQIDQNIAPILLNIGMHCLGCPSSQMETVEE
                     AAMVHGIDPDALVKEINDFLAKDLA"
     assembly_gap    1626..1635
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1636..3052
                     /locus_tag="ACZO9R_05645"
     CDS             <1636..3052
                     /locus_tag="ACZO9R_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellin N-terminal helical domain-containing
                     protein"
                     /protein_id="HIR0912582.1"
                     /translation="INHAKDNPAGMAISRKMKAQIDGLDRASANASDGISVIHISDGA
                     LGEVTSILQRMRELSVQAASDATMGQEDKEAIQKEISSLKDEVDRISKDTEYNTKTLL
                     DGSLDTRVYTKHASRIQVTDQVSPGNYEIKVKTAATQAEVTSGADFNSTAAIGVSGTM
                     SVNGSTVEIAATDTYQEVYEKLRTAGEIGETQIERSKADGSLTFTTDAYGSDATLKLS
                     FSNQALANQFALSDASGNNGLVQDPATKQWIYGSQQTDASGKTTTSVPNGSDMELDDV
                     ANWKGFTSSATAKCDGNRVTITDLGGFSLSFLVEAGYEDGATKTDSAGNVLETYDGSL
                     KFEVTDMGTMTLHIGANMDQNMNIRIPEISCESLYIDDLDVTTVNGADRALDRLDDAI
                     ARVSETRSNLGAYENRLEYSTNSLDEFGENMTDAMSRLTDVDMAEEMTNYTHQNVLNQ
                     AAISVLTQANDLPQQVLQILQ"
     gene            3084..>3368
                     /locus_tag="ACZO9R_05650"
     CDS             3084..>3368
                     /locus_tag="ACZO9R_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HIR0912583.1"
                     /translation="MKKEQIVDFTRRISQANRSGLVVVMYDIFFTYLDDARAAYDAAD
                     WDAYKEALRRAQRAIDELISALNFSYDMAKNLYSIYVFCRDSIAKSLYKRD"
BASE COUNT          938 a          699 c          951 g          760 t
ORIGIN      
        1 agctgcttcc gcgaggaagt cagattccag ggagaaagaa gatctatccg ccagagatct
       61 ggggattcgc ttgatggccc gataaatctt cagcttgaga tgctcggcga gaaatacggt
      121 accgaaacta ccggaaccga gtacggaaag aatccggtac ttgccgaaca gaataacttc
      181 ttctatgcaa aatccgctcc tttcatagtg tggcagattc gcatctcatg catatgttta
      241 taacatgaaa aatgtagttn nnnnnnnnnt gcgggagttt cgcatgaaag tttggctgga
      301 agcttggctt tcagaatacg gaaaggcgtg gtgtagtatg aaaattgaaa aagtaaacga
      361 caatcagatt cgctgtacgc tgacgaggga ggatctggca gagcgtcaga tcaagctgag
      421 tgagcttgca tacggaacgg agaaggcaaa gcttctgttc cgcgatatga tgcagcaggc
      481 ggcgtatgaa tttggttttg aggcggatga tattccgctg atgatagagg caataccgtt
      541 gtcacaggac acgattattc tggtgattac caaggtggag tatccggagg agcttgatac
      601 cagattttcc aggttctccg atccggatcc ggaagacgtg tacgaggagg caatggcagg
      661 cggtggagca gcaccggcgc cggaaggtgc agatgacata ctggggttgt tccgcaggat
      721 tcaggaagag cgcagaaagg cggcagatgc acagggcagt aaggatagct tcgtttcaga
      781 agagaatgat aagaaaaaag aaactccggt aaacgtactt gtagacatta cgaagttatt
      841 tgtgttcagt gatttttctg agataaccag actggcgcac acgctgcagg gcttctatca
      901 ggggaataac gatctgtata agaatgaggc ggcgcacaag tattatcttc tggtcagcaa
      961 gagcagacag acaccggaag agttcaacaa ggtgtgcaat attttatccg aatatggaac
     1021 gcagcagacg tatacgccgg cagcaggcgc gttcatgaaa gagcactatc aggtgatttt
     1081 gaaaaatcat gcgcttcaga cgttggcaga gctttaaagg tcttgcggcg ttaagcaaca
     1141 gaaaaaaggg tattccggtt ccagtgggaa ccgggatacc cttttcgtct ggtgtctgcc
     1201 tgcggattaa gcgagatcct ttgcaagaaa atcgttgatc tcttttacca gagcgtccgg
     1261 atcgataccg tgtaccatgg cagcctcttc tactgtctcc atctgggaag acgggcatcc
     1321 aaggcagtgc attccaatat ttaaaaggat tggtgcgata ttctgatcga tctgaagtaa
     1381 ttcgccaatc atcgtggact ttgtgatctg tgtcataatg atacctcctt atgtgattat
     1441 gggcatgtat gattacatta gtatgcccgg caatgtgcta attataatac gaaacgccgt
     1501 ggaatgcaaa caaagtttgc gtgaaataca caggtgcgcg ggaaatcttt tctgccgggt
     1561 attcagtgaa gcggcaagtt ggtcgatata taggacagat atggataaag gagaacgaca
     1621 ggatgnnnnn nnnnnaatca atcatgcaaa ggataatccg gcgggaatgg cgatttcccg
     1681 caagatgaaa gcacagattg acggacttga ccgggcatct gccaatgcgt cggacggtat
     1741 ttccgtcatt cacatttcgg atggtgcgtt aggtgaggtg acaagtattc tgcagcgtat
     1801 gagagagctt tccgtgcagg cggcgagtga cgcgacgatg ggtcaggaag acaaggaagc
     1861 cattcagaaa gagatttcat ctttaaagga tgaggtggac cgtatttcca aggataccga
     1921 gtacaatacg aagacactgc ttgacggctc gctggacacc agagtgtaca caaagcatgc
     1981 cagccgcatc caggtgacgg atcaggtgag tccaggaaat tacgagatta aagtcaaaac
     2041 ggcagcaaca caggcagagg tgacgtccgg tgcagatttt aacagcacgg cggcaatcgg
     2101 cgtttccggc acgatgagcg tcaatggatc aaccgttgag atcgcggcaa ccgacacgta
     2161 tcaggaagta tacgaaaagc tccggacggc tggagagatc ggcgagacac agatcgagcg
     2221 cagcaaggcg gacggaagcc tgacattcac gacagacgct tatggatctg acgccacatt
     2281 gaagctttcc ttcagcaatc aggcgcttgc caatcagttt gcactgtccg acgcatccgg
     2341 caataacggt ctggtgcagg atccggcgac aaaacagtgg atttacggaa gccagcagac
     2401 agatgcttcc ggcaagacga caacgagtgt tccgaacgga tcggacatgg aattggatga
     2461 cgtggcgaac tggaagggat ttacctcttc cgcgaccgca aagtgcgacg gtaaccgcgt
     2521 gactatcacg gatctgggcg gtttttccct ctcattcctg gtggaagcag gatatgagga
     2581 cggtgcgacc aagacagatt ctgccggcaa tgtgttggag acctatgacg gatccctcaa
     2641 atttgaggtg acagatatgg gaaccatgac gcttcatatc ggtgcaaaca tggatcagaa
     2701 tatgaacatc cggattccgg agatttcctg cgagagcctc tatatcgacg atctggatgt
     2761 gacgacggtc aatggtgcag accgtgcctt agaccgtctg gatgatgcga ttgcaagagt
     2821 cagcgaaaca cgttcaaatc tgggagccta tgagaatcgt ctggagtatt ccacaaacag
     2881 tctggatgag tttggggaaa atatgacgga tgcaatgtcg agacttacgg acgttgacat
     2941 ggcggaagag atgacgaact atacacatca gaatgtgctc aatcaggcgg caatttctgt
     3001 tttgacacag gcaaatgatc tgccgcagca ggtactgcag attctgcagt aggcgcgatg
     3061 aggcatgaga acggaggtct gttttgaaaa aagaacagat tgtagatttt accaggcgga
     3121 tcagtcaggc gaaccggagc ggactggtcg tggttatgta tgatattttt ttcacatatc
     3181 tggatgatgc cagggcggca tacgatgcag ccgactggga tgcctacaaa gaagcattgc
     3241 ggagagcgca gcgcgcgatt gacgaactga tatcggcgct caatttctcc tatgacatgg
     3301 cgaagaatct ctacagtatc tatgtgttct gcagagattc gatcgcaaag tctctctaca
     3361 agagagat
//
LOCUS       DBMDQJ010000221         3361 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10292_length_3361_cov_1.396933, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000221 DBMDQJ010000000
VERSION     DBMDQJ010000221.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10292_length_3361_cov_1.396933"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(9..1025)
                     /gene="galE"
                     /locus_tag="ACZO9R_05655"
     CDS             complement(9..1025)
                     /gene="galE"
                     /locus_tag="ACZO9R_05655"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR0912584.1"
                     /translation="MTILVTGGAGYIGSHTCVELQNAGYDVVVLDNLSNASEKSLQRV
                     EALTGKPVTFYKGDILDRDILNKIFAEHKIDCCIHFAGLKAVGESVAKPWEYYNNNIA
                     GTLTLVDVMRQNGCKNIIFSSSATVYGDPAEIPITENCPKGQCTNPYGWTKSMLEQIL
                     TDIQKADPEWNVILLRYFNPIGAHKSGTMGENPNGIPNNLMPYVTQVAVGKLKELGVF
                     GNDYDTPDGTGVRDYIHVVDLAIGHVKALKKIEEKAGLCIYNLGTGHGYSVLDIVKNF
                     EAATGVKIPYVIKARRPGDIATCYCDPSKAKNELGWEAQYGIREMCEDSWRWQKNNPN
                     GYED"
     gene            1220..2050
                     /locus_tag="ACZO9R_05660"
     CDS             1220..2050
                     /locus_tag="ACZO9R_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR0912585.1"
                     /translation="MPHHYKNSYKVTEKELVSLSVYNVGFQKCDALYQWGPGIRDHYL
                     IHYIISGKGTYRVAGQTYHLSGGDTFLVYPNTEVVYCADEQNPWEYAWVGFTGSDASM
                     ILKATDFSPENPFIRDTPRGNDIHRQILHIYDARGNEFEHAVEMTGRLYTMLALFMHG
                     ATHAGSQNSANSYVQKSIEYISSNYSYAITVEDIAGYVGVSRSHLFRSFEAVLGRSPK
                     EYLTDFRMKQACYLLEHSSLSITAIANSLGFDNSLYFSKTFHRQKGMSPKEYREKNRS
                     "
     gene            complement(2051..2770)
                     /locus_tag="ACZO9R_05665"
     CDS             complement(2051..2770)
                     /locus_tag="ACZO9R_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR0912586.1"
                     /translation="METQRYLYETHMHTSEASACAGSTGAQMARAYKEAGYTGIIVTD
                     HFFYGNTSVDRSLPWEEWVRRFCLGYEHAKAEGERIGLSVFFGWEACYEGTEFLVYGL
                     DQAWLLAHPQIKDASIEEQYALVHEGGGIISHAHPFREEDYIPEIRLFPEYVDAVEGV
                     NAMHSSRASDCHKNPLFNERALAYGKEHNLPFTAGSDQHTTHMIGGGMVFPRKLKDVH
                     DFTRAVLGREAVELLDGCKSE"
     gene            complement(2787..3236)
                     /locus_tag="ACZO9R_05670"
     CDS             complement(2787..3236)
                     /locus_tag="ACZO9R_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HIR0912587.1"
                     /translation="MGWDHAAEEHKYDDIRMLVHHRSRVHPPMSLWDRAAQFSPFAAL
                     SGHEDAIRETARLTTERAELSESRKEELNEQLVWLREHPDARERVTVTYFRPDAKKAG
                     GAYVTVTGAVKKIDEYEKTLWMEDGTVIAIEELSAVEIPGKDRYEEE"
BASE COUNT          722 a          997 c          740 g          902 t
ORIGIN      
        1 ttcacatctt aatcttcata tccgttcgga ttgttcttct gccatctcca ggaatcctca
       61 cacatctcac ggataccata ctgtgcctcc cagccaagct cgttctttgc cttggacgga
      121 tcacagtagc aggttgcaat atcgcccggt cttctcgcct tgattacata aggaatcttc
      181 acacctgttg cagcctcaaa attcttcacg atgtcaagca cgctgtagcc gtgtccggta
      241 cccagattgt agatgcagag tcccgctttc tcctcaatct tcttgagtgc cttcacatgt
      301 ccgatggcaa gatcgaccac atggatgtaa tctctcacgc cggtaccgtc cggtgtgtca
      361 taatcgttgc cgaatacacc gagttctttt aactttccga cagccacctg tgttacatac
      421 ggcatgaggt tgttcgggat accgttcgga ttctctccca tcgtgccgct cttgtgtgca
      481 ccgatcgggt taaagtagcg aagcagaatc acattccact ccggatctgc cttctggatg
      541 tcggtcagga tctgctctaa catggacttt gtccatccgt acggattggt gcactgaccc
      601 ttcgggcagt tctccgtgat cgggatctct gccggatcgc cgtatactgt cgctgaggag
      661 gagaaaatga tattcttgca gccgttctga cgcatcacat ccaccagtgt cagcgtacct
      721 gcaatgttat tgttgtaata ctcccaaggc tttgccacgg actcgcccac tgcctttaat
      781 cccgcaaaat ggatacagca gtcgatcttg tgctccgcaa agattttgtt caggatatca
      841 cggtctaaga tatccccttt ataaaaggtt accggctttc ctgtcagcgc ctccacacgc
      901 tgtaaggact tctcggaggc attgcttaaa ttatccagca ctaccacgtc atatccggca
      961 ttctgtaatt ctacgcatgt atggctgccg atatatccgg ctccgcctgt tactaaaatc
     1021 gtcataatga ttccaccttt ccccgttatg attcattgtt ccatctgcct tacgaaaatt
     1081 atattactct atcctccgta aaatgtatag cccaaaatgt atgaaatata tgtaataatg
     1141 ttgcttttcg gcttttctgt ttttataatg gacttatacg ataaggattt tactgtcaga
     1201 aagacaagga gtgtgttcta tgccgcatca ttacaaaaat tcctacaaag tgaccgaaaa
     1261 agaactggtt tccctctccg tctacaacgt cggcttccag aaatgtgacg ccctctacca
     1321 atggggtccc ggtatccgcg atcactatct gatccactat atcatttccg gaaaaggcac
     1381 ctaccgcgtc gccggtcaga cttaccatct gtccggcgga gatacttttt tggtctaccc
     1441 aaacaccgag gttgtctact gcgcggatga gcagaacccc tgggagtatg cctgggtcgg
     1501 ttttaccgga agcgacgcct ccatgatcct gaaggccacg gacttctccc ctgagaatcc
     1561 ttttatacgg gacaccccac gcggcaatga tattcaccgg cagattctgc acatctacga
     1621 tgcacgcggc aacgaattcg agcacgcggt ggagatgaca ggacgtctct ataccatgct
     1681 cgccctgttc atgcacggtg ctacgcacgc cggcagccag aattccgcga acagctatgt
     1741 acaaaaaagc attgagtaca tctcctcaaa ttattcctac gccatcaccg tggaggatat
     1801 tgctggctat gtcggcgtga gccgcagtca tctgttccgc tcgtttgagg ctgtcctagg
     1861 gcgctccccc aaggaatacc tgacggattt ccgcatgaag caggcgtgct atctgctgga
     1921 gcactccagc ctgtcgatca ccgcgatcgc aaactcgctg gggtttgaca acagtctcta
     1981 tttttccaaa acctttcacc ggcagaaagg catgtcgccc aaagaatacc gggaaaagaa
     2041 ccgctcgtaa tcactcgctt ttgcagccgt ccaacagttc caccgcctct ctgccaagca
     2101 ccgcgcgggt aaagtcatgg acgtctttta gttttctggg aaagaccata ccgccgccga
     2161 tcatatgcgt ggtgtgctgg tctgatcccg ctgtaaatgg cagattgtgc tcttttccat
     2221 aggccagagc ccgctcgtta aacagcgggt tcttgtggca gtcgctcgcc ctgctcgaat
     2281 gcatggcgtt tacgccctcc accgcatcca catattccgg aaacagccgg atttcgggaa
     2341 tgtaatcctc ctcccggaac ggatgcgcat ggcttataat gccgccgccc tcatgtacca
     2401 gcgcgtactg ttcttcgatc gatgcatcct taatctgcgg atgtgcaagc agccatgcct
     2461 gatccagtcc gtataccaaa aactccgtcc cctcataaca tgcttcccat ccaaaaaaca
     2521 ccgagagccc gatgcgttcc ccctctgcct ttgcatgctc atatccgaga cagaagcgcc
     2581 gtacccactc ctcccacggc agggaacggt ccaccgaagt atttccgtaa aaaaagtgat
     2641 ccgttacaat aatgccggtg taccctgcct ctttgtaggc gcgcgccatc tgggcacccg
     2701 tgctgccggc gcatgcgctt gcctccgatg tatgcatatg tgtctcatac aggtatcttt
     2761 gtgtttccat cttcatgctg ctccttctat tcttcctcat atcggtcctt ccccggtatc
     2821 tcaaccgcag acagttcctc aatcgctatg acggttccat cttccatcca aagcgtcttt
     2881 tcgtattcgt ctatcttttt taccgctccg gtgactgtca cataggcacc gcccgctttt
     2941 ttcgcgtccg ggcgaaaata ggtcaccgtc acccgctctc tcgcatccgg gtgctcccgc
     3001 agccagacga gctgctcgtt taactcctct ttgcggctct cggaaagctc tgcgcgctcc
     3061 gtcgtaagac gtgccgtctc gcggatcgcg tcctcgtgtc cgctcaacgc ggcaaacgga
     3121 gagaactgcg ccgcccgatc ccacagggac atcggcggat gcacgcgcga tctgtgatgc
     3181 acaagcatcc gtatatcgtc atatttgtgt tcctctgccg catgatccca gcccattatg
     3241 ccttatgccc tccaatctgg ctgttcctgt ctcttgctgt tgcgccttct tcaaaattca
     3301 tgcccttgag aagtgcgttc ttgccgaatt ttttctttat ctcaatggta gcctcctgga
     3361 t
//
LOCUS       DBMDQJ010000222         3357 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10312_length_3357_cov_1.586916, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000222 DBMDQJ010000000
VERSION     DBMDQJ010000222.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10312_length_3357_cov_1.586916"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(91..1257)
                     /gene="mutY"
                     /locus_tag="ACZO9R_05675"
     CDS             complement(91..1257)
                     /gene="mutY"
                     /locus_tag="ACZO9R_05675"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR0912588.1"
                     /translation="MLDNYHLNQLAEPLLVWFLDHARVLPWRERPEPYRVWVSEIMLQ
                     QTRVEAVKPYYERFLKALPDVAALAACPEDQLMKLWEGLGYYNRVRNMQKAAVTVTEE
                     YGGVLPADYGKLLELKGIGSYTAGAIASIAYGIPVPAVDGNVLRILTRVAADDTDIMK
                     QSFRSAVEAQLGEMMRELTVPEELRRALRDENVPGALNQAMMELGATVCVPNGAPLCE
                     ECPWSKMCLARAQGRVMDFPVKSKAKARRIEERTVLVIRDGEKVAIRKRPKKGLLAGL
                     YELPNLSGALSAEEVLSYVKEQQFAPLRIEKLADAKHIFSHIEWRMSGYVILVEEAGF
                     ATEKEPESGNGRTPEEKADGEGGLIFVDAWDARERYAIPSAFSAYAGYLKKEFD"
     gene            complement(1278..2465)
                     /locus_tag="ACZO9R_05680"
     CDS             complement(1278..2465)
                     /locus_tag="ACZO9R_05680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0912589.1"
                     /translation="MSTKRSYLSKVVVEMPDSGIKDFFDIANTMDGALSLGVGEPDFQ
                     TPEHVREAAVASIRRAETKYTDNRGTVELRAAAAEYLEKFDLHYDRADEILITMGASE
                     GIDLALRTLVDPGDEVLVVEPAYVSYIPCVELCGGIPVSIPLQAKNGFRLTAEELEEK
                     ITGRTKVLLISFPNNPTGAIMEKADLEAIREVVLAHDLFVITDEIYAELTYGRKHVSI
                     AALPDMYERCVVLNGFSKAFAMTGWRMGIAAGPAEVIFHMNKIHQFTTMSAGTTSQYA
                     ALEALTSPVRDEEIDVMRQEYDERRHLMVDGFRAMGLKVVEPEGAFYVFPSIKETGLT
                     SMEFCRRLLQEQKVAVIPGDAFGSCGEGYIRCSYAYSKDVIKKCLEKIAAFVAQFDLE
                     QNA"
     gene            2625..>3357
                     /locus_tag="ACZO9R_05685"
     CDS             2625..>3357
                     /locus_tag="ACZO9R_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR0912590.1"
                     /translation="MSEFNNLKLEIADQTAVLSISRPAALNALNSETLDELNTALTEI
                     EAMDDVKVIILTGGPDKKGNEFKSFVAGADISEMVNFTAPEARAFGIKASVPFFKLMN
                     MRQVTIAAVNGFALGGGCEIAMACDIRIASDNAVFGQPECGLGIIPGFGGTQRLARLV
                     GMGRAKEMIFTCDNIDANEAYRIGLVNKVVAKEDLMATAKAMAAKIISKGSYAVSVAK
                     AAINNGYDMDIKNAVEMEANLFGVVN"
BASE COUNT          769 a         1023 c          738 g          827 t
ORIGIN      
        1 actttccata cacacaatct ttttcatatg aaaagccggt gcgatgcacc ggctccctct
       61 tttttacaaa cttttttatc tttcttgatc ttaatcaaat tcctttttta aataacccgc
      121 atatgctgaa aatgccgacg gaatggcata acgctctctc gcatcccatg catccacaaa
      181 aatcagtccg ccttccccgt ccgctttctc ttccggggtg cgcccatttc ctgactccgg
      241 ttccttttct gtggcaaagc cggcttcctc caccagaatg acatatccac tcatcctcca
      301 ctcgatatgg gaaaatatgt gctttgcatc cgcaagcttc tcgattcgca gcggagcaaa
      361 ctgctgttcc tttacatagg aaaggacttc ctccgcactt aatgcgccag acagattcgg
      421 cagctcatac agtccggcga gcagcccctt cttcgggcgc ttgcggattg ccaccttttc
      481 tccatcccgg atgacaagca ccgtgcgctc ctcaatgcgg cgcgccttcg ccttgctttt
      541 taccggaaaa tccatcacac gcccctgtgc tctcgcaagg cacatcttac tccacggaca
      601 ttcctcacag agaggtgcgc cgttcggcac acagaccgtc gccccgagct ccatcatcgc
      661 ctggttgagc gcgccgggaa cattttcatc gcgaagggca cgccgaagct cctccggaac
      721 cgtaagttcc cgcatcatct cccccagctg cgcttccacc gcactgcgga acgactgttt
      781 catgatgtcg gtatcatctg ccgccacgcg cgtcaagatc cgcagcacat ttccgtctac
      841 cgccggcacc ggtatcccat aggcgatcga tgcgatggca cccgctgtgt aggaaccaat
      901 tccttttaac tccagaagct tcccataatc cgccggaagc actccgccat actcctccgt
      961 cacggtaact gcggctttct gcatatttct cacacggttg taatagccaa gcccctccca
     1021 gagcttcatc agctgatcct ccggacaggc tgcaagtgcc gccacatccg gcaatgcttt
     1081 taaaaatcgc tcataatagg gctttaccgc ctccactctg gtctgttgca gcatgatttc
     1141 cgagacccac acgcggtaag gctccggtct ctcacgccac gggagcaccc gcgcatgatc
     1201 cagaaaccac acgagcaggg gctctgccaa ctgattcaga tgataattat ccagcattct
     1261 cttctccgtc tctactgtca cgcattctgt tccaggtcaa actgcgccac aaacgccgca
     1321 attttttcca gacatttctt tatcacatcc ttggaatacg cataggaaca ccggatgtat
     1381 ccctcgccgc agcttccaaa ggcatctccc ggtatcaccg ccaccttctg ctcctgcaaa
     1441 agtcttctgc agaactccat tgaggtaagc ccggtctctt tgatcgaagg gaacacatag
     1501 aacgctccct ccggctcaac caccttaagt cccatcgccc ggaatccgtc caccatcagg
     1561 tggcgtctct cgtcatactc ctggcgcatc acatcgatct cctcgtcccg caccggactc
     1621 gtgagcgctt ccaatgccgc atactgggat gtcgttcccg ccgacatcgt cgtgaactga
     1681 tggatcttgt tcatatggaa aatgacctcc gccggccccg ccgcgatacc catacgccat
     1741 ccggtcattg caaacgcctt tgaaaaaccg tttagaacca cgcagcgctc atacatatcc
     1801 ggcagcgccg cgatcgatac atgctttctg ccgtaggtga gctccgcata gatctcgtcc
     1861 gtaatcacaa agaggtcatg ggcgagcacc acctcgcgga tcgcctccag atccgccttt
     1921 tccatgatgg cgcccgtcgg gttattcgga aaagagatga gaagcacttt tgtccgccct
     1981 gtgatcttct cctccagttc ctccgccgtc agacggaatc cgttcttcgc ctgcagtgga
     2041 atcgataccg gaattccccc gcacagctcc acgcagggaa tgtaggacac ataggcgggt
     2101 tcaaccacca gcacttcgtc tcccggatcc accagcgtac gcagcgccag atcaatcccc
     2161 tcgcttgcac ccattgtaat aaggatctca tccgcccggt catagtgcag atcgaacttt
     2221 tccagatact ccgctgccgc cgcacgcagc tccacggtac cgcggttgtc ggtgtacttc
     2281 gtctcggcgc gccggatgga ggcgaccgcc gcctccctga catgctccgg cgtctggaag
     2341 tccggctccc cgacgccaag agacagagcc ccgtccatgg tatttgcaat atcgaaaaaa
     2401 tccttaatac cggaatccgg catctccaca acaacttttg acagataaga tctctttgtg
     2461 ctcatatgta tgactcctta tcaatgtact cattttagga cttattatac cccctttctt
     2521 tccaaatgaa aatactgttt tctattgaca aattttagaa aacgctgtac tctaatatta
     2581 gtaatttgtt aattttttat caaactgttc aggagggttt tagaatgagt gaatttaaca
     2641 atttgaaatt ggaaatcgct gaccagactg ctgttctttc catttccaga ccggcagctc
     2701 ttaacgcatt aaacagtgag actcttgacg agttaaacac ggctctgact gagatcgaag
     2761 ctatggacga tgtcaaggtt atcatcttaa ccggcggtcc ggacaagaag ggcaacgagt
     2821 tcaagtcttt cgttgcaggc gctgatatct ccgagatggt gaacttcacc gctccggaag
     2881 caagagcatt cggcatcaag gcttctgttc catttttcaa gttaatgaat atgcgtcagg
     2941 ttaccatcgc ggctgtcaac ggctttgctc tcggtggcgg atgtgagatc gctatggcat
     3001 gtgatatccg tatcgcatcc gacaacgctg ttttcggaca gccggagtgc ggtcttggta
     3061 tcatccctgg attcggcggt acacagagac ttgcccgtct ggttggtatg ggacgtgcca
     3121 aagaaatgat ctttacctgt gacaatatcg atgccaacga agcataccgc atcggccttg
     3181 taaataaggt tgttgcaaaa gaagatctga tggctaccgc aaaggctatg gctgcaaaga
     3241 tcatctccaa gggaagctac gcagtctccg ttgccaaagc agcaatcaac aacggctacg
     3301 acatggacat caagaatgcc gttgagatgg aggcaaacct ctttggtgtt gtcaacg
//
LOCUS       DBMDQJ010000223         3342 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10363_length_3342_cov_1.638692, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000223 DBMDQJ010000000
VERSION     DBMDQJ010000223.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10363_length_3342_cov_1.638692"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="ACZO9R_05690"
     CDS             <1..493
                     /locus_tag="ACZO9R_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR0912591.1"
                     /translation="AQGDRNLAPLPDAPADRYAWGYANAPIGGVNPRIGSTVSNDLTA
                     SREGYIPLFIQEKCINCGLCDTACPDMVFQFAPGTYRGKNCMVNRGLDYYHCKGCLRC
                     VEVCPTGALVPALEKDYPEKPHFLPNQHLLPDAIRYQTVGANSWITSDSFTDEKRVDG
                     GVV"
     gene            552..3029
                     /locus_tag="ACZO9R_05695"
     CDS             552..3029
                     /locus_tag="ACZO9R_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016282915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR0912592.1"
                     /translation="MEKQKIMYDSGNELAAFAAKQINYHVMGYYPITPSTQIAENLDV
                     MGAEGLHDIALIAAEGEHSAAGICYGASAAGGRVFNATSANGLLYALEQFPVQSGTRM
                     PMVMNVACRTVSGPLCIKGDHSDVMYLLNTGWIILFADEPQKVYDFNLLGLKLAEAVR
                     LPVAVAFDGFFTSHQKRKCLVFENDDTVTRYIGEKLSCDNPKVSAFAGTGTCGAAGEL
                     PYASVLDLAHPVSIGSYMNEPDVINNRYQLHLAMEAARNKLPELFTEYAALSGRELSL
                     CGAYRHEDAEVLLFVLGSSYHTAMEAVDRLRQDGIAAGVITLYVLRPFPAKELRVLCH
                     NASTILAADRQDSYGAGGGNMSLELKAALSSLPHPPRILSRIYGLGGKDFFVEDALAL
                     FKEALSPDAPAFDYYGVTAGTEASDEADSAGTSFSGTDAVTAVSHQAASINEDMISSA
                     SGRADRTIADQASGTSGKADQSMAAPAMQPQYFKPVTKEESSPGLTTCTFDPATGKMK
                     VSGGSVKDTTAMPMRVAPGHGACPGCGIPINVNLLLKGIEGNVVLLFQTGCGMVVTTG
                     YPKTAFRVPFLHNLFQNGAATLSGVVEAFHQRQKRGEYPDGEITFVMVSGDGGMDIGM
                     GSALGTALRGHKLIIFEYDNGGYMNTGYQLSYSTPLGAKSSTSHVGKTQYGKNFFHKD
                     TPELMAATHIPYVATVAESNPADFIRKAAKAAAYSREFGTAYIKALSACPLNWNDKPN
                     LERSVIAAAVDCCYFPLYEIERGITALNYDPASSNKKIPVTEWLGMMGRTRHLLKEEY
                     RSVTEEIQKEIDRRYDRLKARAEHPLL"
BASE COUNT          738 a         1007 c          906 g          691 t
ORIGIN      
        1 ggctcagggc gaccgcaacc tcgcccctct gccggacgcc ccggcggacc ggtatgcttg
       61 gggctatgcc aacgctccga tcggcggcgt caatccgcgg atcggaagca ccgtctccaa
      121 cgatctgacc gcctcgcgcg aagggtacat tccccttttt attcaggaaa aatgtatcaa
      181 ctgcggactg tgtgacacgg catgcccgga tatggtcttc cagtttgctc ccggcaccta
      241 ccgcggaaaa aactgcatgg tcaaccgggg gcttgactac tatcactgca agggctgcct
      301 gcgctgtgtg gaagtctgcc ccaccggcgc actcgtgccg gcactcgaaa aagattaccc
      361 ggaaaaaccg cattttcttc cgaatcagca tctgctgccc gacgccatcc gctatcagac
      421 cgtcggtgcg aattcctgga tcacaagcga ttcttttacc gacgaaaagc gggtcgacgg
      481 aggcgtcgta taaatgcagg cagcacccgt gaatcgtacc ggcgcacagc gtctgcagaa
      541 tggagaatga tatggaaaaa caaaaaatta tgtatgacag cggcaatgag cttgctgcat
      601 ttgccgcaaa gcagatcaac tatcacgtca tggggtatta tccgatcacg ccctctacac
      661 agattgcgga aaatctggat gtcatgggcg cggaggggct tcacgacatt gccctgatcg
      721 ccgccgaggg agaacacagc gccgccggca tctgctacgg cgcctcagcc gctggaggcc
      781 gtgtatttaa tgccaccagc gccaacgggc ttttgtacgc cttagaacag tttccggtgc
      841 agtccggcac acgcatgccg atggtcatga atgttgcatg ccgcacggtt tccggccctt
      901 tgtgcatcaa aggcgatcac agcgacgtca tgtatctgct caacacaggc tggatcattc
      961 tttttgccga tgaaccgcag aaagtctatg attttaacct gctcggtcta aagcttgccg
     1021 aggctgtccg tctcccggtc gcagtcgcgt tcgacggctt ttttacgagc catcagaagc
     1081 gcaagtgcct tgtatttgag aatgatgaca ccgtcacccg ctatatcggg gaaaagcttt
     1141 cctgtgacaa cccgaaggtc tccgcatttg ccggcaccgg cacctgcggc gccgccgggg
     1201 agcttcccta cgccagcgtc ttagatctcg cgcatccggt ttccatcggt tcctatatga
     1261 acgagccgga cgtcatcaat aaccgctacc agctccatct cgccatggaa gccgcgagaa
     1321 acaagctgcc ggagctgttt acggaatatg ccgccctctc cggcagggaa ctttccctct
     1381 gcggtgccta ccggcacgag gacgccgagg tactgctttt tgtgctcggt tcctcctacc
     1441 ataccgccat ggaagccgtc gaccggctgc ggcaggacgg catagcggca ggcgtgatca
     1501 ccctctacgt tctgcgcccg ttccccgcta aggaacttcg ggtgctctgc cacaatgctt
     1561 ccacgattct ggcagcagac cggcaggaca gctacggcgc cgggggcggc aacatgtcgc
     1621 ttgaattaaa ggccgctctc tcttccctgc cgcacccgcc gcgcatttta agccggatct
     1681 acggtctcgg cggcaaggac tttttcgtgg aggacgccct cgccctcttt aaagaggcac
     1741 tctcgccgga cgccccggcg ttcgattact acggcgtcac tgcgggcacg gaagcttcgg
     1801 atgaggcaga ttccgcaggg acatctttct ccgggacaga cgcggtgacg gcggtttcac
     1861 accaggcagc ctctatcaac gaagatatga tttcaagtgc ctccggcagg gctgaccgca
     1921 caatagcgga ccaggcttcg ggcacttccg gcaaggctga ccagtcgatg gcagctccgg
     1981 cgatgcagcc gcagtatttt aagcctgtca caaaggaaga atcctcgccg ggtctcacca
     2041 cctgcacctt tgatcccgca accgggaaaa tgaaagtctc cggcggctcc gtaaaagata
     2101 cgaccgccat gccgatgcgc gtcgcaccgg gacacggtgc ctgcccgggc tgcggcattc
     2161 cgatcaatgt gaacctcctg ttaaagggca tcgagggaaa tgtcgtcctg ctgtttcaga
     2221 ccggctgcgg catggtagtg acaaccggct accccaaaac cgcgttccgc gttccatttc
     2281 tgcacaatct gtttcaaaac ggcgcggcga ccctaagcgg cgttgtggag gcgtttcacc
     2341 agcgccagaa gcgcggggaa tatccggacg gcgagattac ctttgtgatg gtgagcggtg
     2401 acggcggaat ggatatcggt atgggatcgg cactcggcac cgccctgcgc ggtcataagc
     2461 tgatcatttt tgagtacgat aacggcggct acatgaatac cggctaccag ctctcctact
     2521 ccacaccgct cggcgcaaag agttctactt cccatgtcgg aaagacgcag tacggcaaga
     2581 acttcttcca caaggacaca ccggagctga tggccgcaac ccacattccc tatgtcgcca
     2641 cggtcgcaga atcaaatccg gccgatttta tccggaaagc ggcaaaagcc gccgcctatt
     2701 ccagagagtt tggcactgcc tatatcaaag cgctgtccgc ctgtccgctg aactggaatg
     2761 ataagcccaa tctggagcgg agcgtcattg ccgccgccgt ggactgctgc tattttccgc
     2821 tctacgaaat cgaacgtggc atcacagcgc tgaattatga tcctgcttcg agcaataaga
     2881 aaatccctgt cacagaatgg ctgggtatga tgggacgcac caggcatctg ctgaaggagg
     2941 agtaccgctc cgtgaccgag gagatccaga aggagatcga ccggcgctac gaccgcctta
     3001 aggcgcgcgc agagcatccg cttctgtagg cacacagaga gaatccggtc ggcgggatgc
     3061 tgtgtccgga agctcatagc cagaccggta attgaaaaag gggcaggaat ttcttcctgc
     3121 cgcgctcccc atttcattcc atgtctcttg ccacgcttta acactccgtg ggtgtgccca
     3181 cagccgcgga gcgctcaaag cccggataga cacggagcat cacaaatcaa aaggcaaaag
     3241 gaaattcgaa ctcttctcct acagaaaatg gaaattcccc gtatatttcc gtaactttaa
     3301 aacttgcgcc attccatccg tcgattttga tcattccttt ta
//
LOCUS       DBMDQJ010000224         3341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10365_length_3341_cov_1.727469, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000224 DBMDQJ010000000
VERSION     DBMDQJ010000224.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10365_length_3341_cov_1.727469"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            56..1843
                     /locus_tag="ACZO9R_05700"
     CDS             56..1843
                     /locus_tag="ACZO9R_05700"
                     /inference="COORDINATES: protein motif:HMM:NF021126.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HIR0912593.1"
                     /translation="MLKFGLTSLLSLGIAAVIFLPAARAMLGSYRLSGEEYRGISGYF
                     LLLRQDGMDAVWKWVILGSVAVPLLFFVISGRRKREHIWQWYLVAAMVLPVFFEAVNL
                     IWHRGTYVCFPMRYGYMLVFAVICMAAARVPDFVGESRTRRNILYAFLGIWCVQMVAL
                     GIALIQPEPHMDEADFVGDATEIGQPLAGKTDIFRKAKLADGAIDNNYPLIADNTSYS
                     NYLHLMTSEQIGMNTLLGYSQVWTRLSDTGGTLASDIMLGYRYYVASKAGSWGKDTAE
                     DFLWPLAETEKFTLWENASVGDRAFFITKANYEAYAATEAYTENVFENQNRLSELLFH
                     TTPIDWEKETYVDDETGAELSYRFHADGKQILYLYGKDLQQAEITVNGKRLYVPDYND
                     LYNESYPATGNGGILSIGCFADEDITVDIRQSVGNSGAERAVFFGLLDPQELLEAVDT
                     AGRSVTYELDRDSCKLTVESAGEEYLMLPINADGGWHCTVNGEERTISRLAGNLMLVP
                     LDAGTNEITLCYVPRGMKKGAAVTGIALMLLAGWGFLSRLLRRESVLDKIYAGLGYAA
                     TGIFAVVYAGFLLVVYVIPVVYTIYLRWI"
     gene            1853..3337
                     /locus_tag="ACZO9R_05705"
     CDS             1853..3337
                     /locus_tag="ACZO9R_05705"
                     /inference="COORDINATES: protein motif:HMM:NF039639.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HIR0912594.1"
                     /translation="MEKLQARKKVYLWLLILLSLCALIPLAVLSKYNHQSGDDYGYAY
                     RTFQIWRESHSFWNVIKEAFATSAEFYQKWQGLYASAVLLALEPGIFGDKYYALTGIL
                     LLGIIVGSNCAGTVYIFHRKCGCRKLEGFAVGCVMSFLMIEWMPSALQGLYWYNGAMN
                     YVFFHAVLLLLLCVLLSLAGEHPRRRAGRTVIAVLLAVLLAGGNHVTAFMGILLIAGF
                     GIFAAVKKRWQNLICCAITLVAILAGFLFNVTSPGTRIRQSYFSDRPGVAGTIVTAVK
                     NGIACINSWMGLAVIVTAVLLLPVLLSMVTKIRGQYGFSFRYPLLVFVLSVAWLCAMF
                     CPPIYAMGTPGDVRLTNVVYFSFVWLFVLNEAYLCGWVVTKLEASGAAGLCSRAGELT
                     DSFLITAAVLAVGMIIGCGENMTAWQAYLLIRSGEAQEYSAEADERYAVLCDSAGEDV
                     VLKSYTAKPWMLFLDDITEEKSDWRNEYMRVYFGLNSVVLEQTE"
BASE COUNT          733 a          730 c         1047 g          831 t
ORIGIN      
        1 actatgatgg aaaacaagta attgttaaaa aagaagatta attaaaaaga acagcatttt
       61 aaaattcggg ctgacttctt tactgtcgct tggaattgcg gcggtaatat ttctgccggc
      121 agcgagggca atgcttggtt cctaccggct gtcgggcgag gagtaccggg gaatctccgg
      181 ctattttctg ctgcttcggc aggacggaat ggacgcggtg tggaaatggg tgattcttgg
      241 cagcgtggcg gttccgctgc tgttcttcgt catttctggc agacggaagc gggagcacat
      301 ctggcagtgg tatctggtgg cggcgatggt gctgccggtt ttcttcgagg cggtcaatct
      361 catctggcac cgcgggacgt atgtgtgctt cccgatgcga tatggctata tgctggtgtt
      421 tgccgtgatt tgtatggcag cggcgcgcgt gccggatttt gtgggggaga gccgcacgcg
      481 gcggaacatt ttatatgcgt tccttggaat ctggtgcgtg cagatggtgg cgctcggcat
      541 cgctttgata cagccggagc cgcacatgga cgaggcggat tttgtcgggg atgccacgga
      601 aatcggtcag ccgcttgccg ggaaaaccga tattttccgc aaggcaaagc tggcggacgg
      661 agcgattgac aacaattatc cgctgattgc agataacacc tcctactcta actatctgca
      721 tctgatgaca tcggagcaga tcgggatgaa cacgctgctc ggatattctc aggtgtggac
      781 cagattaagt gacacgggcg gaaccctcgc gtccgatatc atgctgggat accgctacta
      841 tgtggcgtcc aaagcgggct cgtggggaaa agacacggcg gaggattttc tttggccgct
      901 tgcggagacc gaaaagttta cgctgtggga aaatgcgtcc gtcggggaca gggcattttt
      961 tatcacaaag gcgaactatg aagcgtatgc agccacggaa gcgtacacgg agaatgtgtt
     1021 tgagaaccag aaccggttgt cggagctgct ctttcacaca actcccatcg actgggaaaa
     1081 agagacctat gtggatgatg agaccggagc ggaattatcg taccggtttc acgcagacgg
     1141 aaagcagatc ctatatctct atggaaagga tctgcagcag gcagaaatta cggtaaacgg
     1201 aaagaggctc tacgtcccgg attacaatga tctgtacaat gagagctatc ctgcaaccgg
     1261 caatggggga attctctcca tcgggtgttt tgcggacgag gacatcacgg tagatatccg
     1321 ccagtccgtc gggaacagcg gcgcagagag agcggtgttc ttcggactcc tcgatccgca
     1381 ggaactgctg gaggcggtgg atacggcggg aaggtcggtt acatatgaac tggatcggga
     1441 ttcctgtaag cttactgtgg aatcggcggg agaggagtat ctgatgcttc caatcaatgc
     1501 ggatggcgga tggcattgta cggtaaacgg agaggagcgg accataagcc ggctcgcggg
     1561 caatctgatg cttgtgccgc ttgatgcggg aacgaacgag atcacgcttt gttatgtgcc
     1621 gcggggaatg aaaaagggcg cggcggtgac gggtatagcc ctgatgcttc tggcaggctg
     1681 gggctttctc tcgcgtctgc ttcggagaga aagcgtcctg gataaaatct acgccggact
     1741 tggctatgcc gccacaggta tttttgcggt ggtctatgcc ggatttctgc tggttgtcta
     1801 tgtgattccg gtggtgtata cgatatattt aagatggatc tgaggtaaca gaatggaaaa
     1861 attacaagcg agaaaaaaag tatatttgtg gctgctgatt ctattgagtc tttgcgcgct
     1921 gatcccgtta gccgtactct cgaagtacaa tcatcagtcg ggggatgact acggttatgc
     1981 ctaccgtacc ttccagatct ggcgggagtc acattctttc tggaatgtca taaaagaagc
     2041 gtttgccaca tccgcggagt tctatcagaa gtggcagggg ctatatgcat cggcggtgct
     2101 gctggcgctg gaaccgggaa ttttcggcga caaatattat gcgctgaccg gaattttgct
     2161 gctcggaatc attgtggggt caaactgcgc gggtacggtt tatatctttc accgtaaatg
     2221 cgggtgccgg aaactggagg gatttgctgt cggctgcgtc atgtcgtttc ttatgatcga
     2281 atggatgccc tccgccctgc agggattgta ctggtataac ggggcgatga attatgtatt
     2341 cttccatgcg gtactgttat tgctgttatg cgtgctgctt agccttgccg gggagcatcc
     2401 gcggagaagg gcaggcagga cggtgattgc ggtattgctt gcggtgctgc ttgccggcgg
     2461 gaatcatgtg accgcattta tgggaatatt gctgattgcc ggatttggaa tatttgctgc
     2521 ggtaaaaaag cggtggcaga acctgatctg ctgcgccata acgcttgtgg ctatacttgc
     2581 tggattcctt tttaacgtga catcgccggg caccagaatc cggcagtcgt atttttccga
     2641 cagacccggc gtggcgggaa cgattgtcac ggccgtaaaa aacggaattg catgcatcaa
     2701 cagctggatg ggactggcgg tcattgtcac ggcggtcctg ctgctccctg tgcttctttc
     2761 catggtaacg aagatacgcg ggcagtacgg attttcgttc cgctatccgc tgcttgtctt
     2821 tgtgctgtcg gttgcctggc tgtgcgcaat gttctgtccg cccatctacg cgatgggaac
     2881 gccgggggac gtccggctga ccaacgtggt atatttttcc ttcgtctggc tgtttgtgtt
     2941 aaatgaagcc tatctgtgcg ggtgggtggt cacaaaactg gaggcgtccg gggcggcagg
     3001 actgtgtagc agggcaggag aacttacgga cagctttctg atcacggctg ctgtccttgc
     3061 ggtgggaatg ataatcggat gcggggaaaa tatgaccgca tggcaggcat acctgctgat
     3121 ccgcagcggc gaggcacagg aatattcggc ggaggctgat gagcgctacg ccgttttgtg
     3181 tgattctgcg ggggaagacg tggtgttaaa gtcgtatacc gcaaagccgt ggatgctttt
     3241 ccttgacgat attacagagg agaaaagcga ctggagaaat gaatatatgc gcgtctattt
     3301 tggactgaac agcgttgtgt tagagcagac ggaatgagac g
//
LOCUS       DBMDQJ010000225         3341 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10367_length_3341_cov_1.479938, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000225 DBMDQJ010000000
VERSION     DBMDQJ010000225.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10367_length_3341_cov_1.479938"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="ACZO9R_05710"
     CDS             <1..857
                     /locus_tag="ACZO9R_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR0912595.1"
                     /translation="SQNSKGPSRDWLNIVKSTQAKNKINQWFRSELKEENITRGKELL
                     VASCKAKGINFADINKPEYQEKVLRKYGFHDWNSCLATIGHGGLKEGQIVNKMYEEYK
                     RDHLARITDEEVLETIGSENKNPESAKRSKSGIIVKGLYDVAVHFSKCCSPVPGDEIV
                     GFVTRGRGVSIHRTDCINIINLSDMEKERLIDAEWQHDGQDGSNGLYRAEIKIFGNNR
                     TGLLVDITRVFTEREIDIDAIHSKTSKQGVATIDVSFSTKGREELGSVIDKLRQIDSV
                     IDIVRTTG"
     gene            889..1518
                     /locus_tag="ACZO9R_05715"
     CDS             889..1518
                     /locus_tag="ACZO9R_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0912596.1"
                     /translation="MAPLKVEQYVVGPVQTNCYFAVNDETKEMILIDPGANADRLIQI
                     IEEEKLNPVAILLTHGHFDHAGGAAEVAAHFGIKVYAEEHERETLDDPKLNLSGWTGA
                     ETTYRADVFLKDEQEFDLAGFHIRVLFTPGHTVGGCCYYFAYQDALFSGDTLFAQSVG
                     RTDFPKGSASALIRGIKEKLLPLPDEVTVYTGHNDTTTIGTERRYNPYL"
     gene            1686..3158
                     /gene="hemZ"
                     /locus_tag="ACZO9R_05720"
     CDS             1686..3158
                     /gene="hemZ"
                     /locus_tag="ACZO9R_05720"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen dehydrogenase HemZ"
                     /protein_id="HIR0912597.1"
                     /translation="MIEVTLNQPEFEYDIHSLIKAFYPSEYVGVSAEKKEYDEPVTSR
                     MHVEYTDDAITLRWQQGETAPAVERTFAVDPLDRPETKNRLKQNLYELLSDATGQYLP
                     WGTLTGIRPTKIPMKLLEEGKSRDEIYTYMKQTYFASDEKIRLATDIAERELSLLSKI
                     DYDNGYSLYIGIPFCPTTCLYCSFTSYPIASWAKRVDSYLDALEREIEFAAVKFAGRH
                     LNSIYIGGGTPTTLEPYQLDRLIRKIKCSFDLSYCLEFTVEAGRPDSITYEKLKVLRQ
                     HGISRISINPQTMKQETLKLIGRHHTVGQTVESFHMARELGFDNINMDLIMGLPEETL
                     DDVRRTMEQVAELAPDNLTVHSLALKRAARLNMFKEDYKDYKIINTQEHMDLVADCAA
                     QMGLFPYYLYRQKGMAGNLENVGYAKEGMAGVYNVLIMEEKQTIVACGAGASTKRVWT
                     EPNPDGTHRIERAENVKDVAQYIARIDEMIQRKQKLFAEE"
BASE COUNT          938 a          692 c          977 g          734 t
ORIGIN      
        1 cttcgcagaa ctccaaggga ccgagccgtg actggctgaa tatcgtaaag agcacccagg
       61 cgaagaataa gatcaaccag tggttccgca gtgagctgaa ggaagaaaat attacacgcg
      121 gcaaggagct tctcgtggcg tcctgcaagg cgaagggaat caactttgca gacatcaaca
      181 agccggaata ccaggagaaa gtgctgagaa aatatggttt ccatgactgg aattcctgtc
      241 tggcgaccat cggacacggc ggcttaaagg aaggccagat tgtcaataag atgtacgagg
      301 aatacaagcg ggatcatctg gcgcggatca cggatgagga ggttcttgag accattggtt
      361 ctgagaataa gaatccggag tctgcgaagc gttccaagag cggaatcatt gtcaagggcc
      421 tgtatgatgt cgctgttcat ttttccaagt gctgcagtcc ggtgccggga gatgagatcg
      481 tgggttttgt gacgagggga cgcggcgtgt ccatccacag aaccgactgt atcaatatca
      541 tcaacttgtc ggatatggag aaggagcgtc tgatcgatgc ggaatggcag catgacggtc
      601 aggacggcag caatggtctg tatcgcgcgg agatcaagat ttttggtaac aaccgtaccg
      661 gacttctggt cgacatcacg agagtgttca cggagcgtga gatcgatatt gatgcgatcc
      721 actccaagac gagcaaacag ggcgtggcga cgatcgatgt gtccttcagc acgaagggca
      781 gagaggagct gggcagcgtg attgataagc tgcgccagat cgacagcgtg attgatattg
      841 tgagaacgac aggctgatga agcaggaaag gcagacggag ggaaaaagat ggcgcctctt
      901 aaggtagaac agtatgtggt aggaccggtg cagacgaact gctatttcgc ggtcaatgat
      961 gagacgaaag agatgattct gattgatccg ggggcaaatg cggatcgtct gatacagatc
     1021 atagaggaag aaaagttaaa tcctgttgca attttattga cacatggaca ttttgaccat
     1081 gccgggggcg ctgcggaagt cgcagcgcat tttggtatca aggtctatgc cgaggagcat
     1141 gagagagaga ccctcgatga tccaaagctg aatctgagcg gatggacggg agcggagacg
     1201 acctatcgcg cggatgtatt tctgaaggat gagcaggagt ttgatctcgc gggattccat
     1261 atccgggtat tgtttacacc gggacacacg gtgggtggct gctgttatta ttttgcctat
     1321 caggacgccc ttttttccgg ggatacgctg ttcgcccagt cggtcggaag gacggatttt
     1381 ccgaagggga gcgcttcggc gctgattcgc ggcatcaagg agaagttact gccgcttccg
     1441 gatgaagtga ccgtctacac cggacataat gacaccacga cgatcggaac agagcgcaga
     1501 tacaatccat atctgtaagc gtatgatccg ggaaaccaga ggttttccgg acttttgccg
     1561 aagcaaagac gcatatgata taatgaatcc tatcggattc atattagaag ttcctgcgga
     1621 acttcggatg cgcagaaagc agcgcaaaaa gatgatataa tatttagttt agaaaagagt
     1681 cagatatgat agaagtaaca ttgaatcaac cggaatttga gtacgatatc cattcactca
     1741 tcaaggcatt ttatccgtcg gaatacgtcg gagtatccgc ggaaaagaag gagtatgacg
     1801 agccggtgac atcccgcatg catgtggagt acacggacga tgcgataacg ctgcgctggc
     1861 agcagggaga gacggcgcct gcggtggagc gtacctttgc agtcgatcct ttagaccgcc
     1921 cggagacgaa gaaccggctg aaacagaatc tgtatgagct tctgtcggat gcgaccggtc
     1981 agtatctgcc gtggggaacg ctcacgggaa tccggccgac caagattccg atgaagctgc
     2041 tcgaagaggg aaaaagccgg gacgagatct atacgtatat gaagcagact tattttgcgt
     2101 cggatgagaa gatccgcctt gcgacagaca ttgcagagag ggaactctcc ctgctttcca
     2161 agatcgatta tgacaatgga tacagtctgt atattggaat tccgttctgt ccgacgacct
     2221 gtctgtattg ctcgtttacc tcctatccga ttgcatcgtg ggcgaagcgt gtggattcct
     2281 acctggatgc gctggagcgg gagatagagt ttgcagcggt gaagtttgcg ggcagacatt
     2341 taaattccat ctacatcggc ggcggtacac cgacgacatt agaaccttat cagctggaca
     2401 gactgatccg gaaaattaaa tgcagctttg atttgtcgta ttgcctggaa ttcacggtgg
     2461 aagcgggaag accggacagc attacatacg aaaaattaaa ggtgttgaga cagcatggaa
     2521 tctcgcgcat atcgatcaat ccgcagacca tgaagcagga gacattaaag ctgatcggca
     2581 gacatcacac ggtggggcag acggtggaga gttttcacat ggcaagagaa ctcgggtttg
     2641 acaatatcaa catggatctg atcatgggac ttccagaaga gacactggac gatgtgcgcc
     2701 ggacgatgga gcaggtggca gagcttgcac cggacaatct gaccgtacat tcgctggcac
     2761 tcaagcgggc agcgcggctg aatatgttca aggaagatta taaggattac aagatcatca
     2821 acacgcagga gcacatggat ctggttgcgg actgtgcggc gcagatgggg ctttttccat
     2881 attatctgta ccggcagaaa ggtatggcgg gcaatctgga gaacgtcggc tatgcaaaag
     2941 agggcatggc gggcgtgtac aatgtgctga ttatggagga gaaacagacc attgtggcat
     3001 gcggagcggg agcttcgaca aaacgtgtct ggacagagcc gaatccggat ggcacgcacc
     3061 ggattgaacg tgccgagaat gtcaaggatg tagcgcagta tatcgcaagg attgatgaga
     3121 tgatacagcg gaagcagaag ctttttgcag aagaataaaa gggttattta tcctgaaata
     3181 gtacatcaaa aaagtatcca ataatacgga ataacctgtg agatgtattg aaacaaccta
     3241 ataactgtgc cttaaaggac gaattcattt tagcagatga gttcgtcctt ttctgttata
     3301 gtcaattatt ctaggagaaa aagaacatga ataacacagc g
//
LOCUS       DBMDQJ010000226         3335 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10381_length_3335_cov_1.488559, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000226 DBMDQJ010000000
VERSION     DBMDQJ010000226.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10381_length_3335_cov_1.488559"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            637..1179
                     /gene="rbr"
                     /locus_tag="ACZO9R_05725"
     CDS             637..1179
                     /gene="rbr"
                     /locus_tag="ACZO9R_05725"
                     /EC_number="1.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016692 - NADH peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin"
                     /protein_id="HIR0912598.1"
                     /translation="MNKYAGTQTEKNLKAAFAGESQARNSYTFFAEIAKKEGYEQIAA
                     IFEKTADNERAHAQMWGEELGLIGDTGKNLDAAAHGENYEWTDMYEDFAKTAEQEGFA
                     ALAARFRMVGAIEKMHEERYRELLRDVQAQAVFEKSSVKVWECRNCGHIVVGTSAPKV
                     CPACHYPQGYYEVREDVRGA"
     gene            1594..2931
                     /locus_tag="ACZO9R_05730"
     CDS             1594..2931
                     /locus_tag="ACZO9R_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912599.1"
                     /translation="MKRRAMAGLLAMTMAMAATMFAGCGSTAADSQTADAQGSAGTAT
                     EAAKTEKDSGSAAGEGITLTVWAPFTGSDGDVLREIIDNYNETNTDNITVQIDIMDND
                     TLQTKFATAVSTGTGPSFVLVGIEYLHQYEENGLIEDISDFWDTMGIDKSNFYENVLA
                     KSYIDDALYGVPMQYNVQYLYYNKDLFTEAGLDPENPPTTLDELREAAIACTDPSKNQ
                     YGLGLPTDYGYYCEYLWANGGDVINEDATQNLLNSEENIATLEWIQKLATEDGVSPEG
                     LTAADADTMFQSGQLAMYTSGPWNINGLTQLGLNYGITAIPGGSDGAYSPEGGCSYML
                     TKGADEQTKEAVYKFMAYWLSDDVLKEWSNRNGFPVWSYSVLENADIQNNEILSAVSK
                     ASEIGRDWHLGLECGSMIDNDVMKPMMEKILSGADVTQSVQEASDALDAVISD"
     gene            2985..>3335
                     /locus_tag="ACZO9R_05735"
     CDS             2985..>3335
                     /locus_tag="ACZO9R_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0912600.1"
                     /translation="MKVKKRQHRMEHQVKAYAFIAPSLLILTVFVFIPLAGAIGISLM
                     NIDIYMKNISFAGIANYIKMFQDERVANATFHTFYFALLEVPLQMIVALLLLMFMLRN
                     TKLHKLLRTVFYLPY"
BASE COUNT         1040 a          661 c          979 g          655 t
ORIGIN      
        1 aaaaaaatat gctattgcgg gtctgagaga gaaaaaaagc cgtgtcagac ccgcacattg
       61 ctatgcaggc aaggaaaacc gcagaaatat ttgaagaacg ggtttgtggg aatattttac
      121 cggtgggtgc ccgaagagac cggaacagac aacaacaggc tgaaacagcc aggaaaaggc
      181 tgaaaggaga gccaggaaga acccggcaag aacagacgaa cagaaagaga ccggaatata
      241 caacaggctg gaaaaaatag gcaggaaaag aaacgaagta acagacaacg ggctaaaaaa
      301 ataggcggga aagggcagag agaaacagca aaaacagaaa gaagcaggaa cagggaaaaa
      361 gatgtcaaaa agttgccggc aggagaaata aatttcgatg gatcgggcat actagcgcaa
      421 taaggcaaag gcaagaacag caggcaggaa caacaggcag gaatgaaagg ttgaggcaaa
      481 aagcaggagc aaaaagctga agccaaaagt tggagtaaaa gtcggagcaa aaagctgaag
      541 ccagaagttg gagtaaaaag caggaacaaa aagctgaagc cagaagttgg agcaaagagt
      601 tagagcgaga gatcgtgtga aaaaatggag ggttctatga acaagtatgc cggaacacag
      661 acggaaaaga atttgaaagc agcgtttgca ggggaatcac aggcgagaaa cagctatact
      721 ttttttgcgg agatcgcaaa aaaggaaggc tatgagcaga ttgccgcgat ttttgagaag
      781 acggcggata acgagcgcgc acatgcgcag atgtggggcg aggaactggg actgatcggc
      841 gatacgggaa agaatctgga tgccgcagcc cacggggaga actatgagtg gacagacatg
      901 tatgaggatt ttgcaaaaac ggcggagcag gaaggctttg cggcgcttgc agcgcgattc
      961 cgcatggttg gcgcgattga gaaaatgcat gaagagcggt accgggagct cctgcgggat
     1021 gtgcaggcac aggcggtgtt tgagaaaagc agcgtgaaag tgtgggagtg ccggaactgc
     1081 gggcatattg tggtcggaac cagtgcgcct aaggtttgtc ctgcctgtca ttatccacag
     1141 gggtattatg aggtgcggga ggatgtcagg ggagcgtaga agtgtctggc gatttgtggg
     1201 cgttccgtat cagtaatatt tcacaaaatt tatagaccaa atacatgaaa aatatataat
     1261 tgacagaaac tatgctgttg ttataataaa tagtgtgttg agtacacgcg tccgcaaatg
     1321 cgcggacgat atttttacac aaaaatgtac acgtgtacgc aaaacagaaa aaatcacaac
     1381 gaaacaatgt atggggagga agtagtgcaa aaatgcggat gatactgcat tttgcacagg
     1441 gctgtttgat gcggcagaca ccacaaaatg agcccttatc gcagagcaaa cgagatggaa
     1501 gacacgttac gggtgcagat tcattgcccg tgcaaatcag aggttgcatc tgcgattgcc
     1561 tcgacacaaa gtgtctgcag aatggaggat aacatgaaaa gaagagcaat ggcgggatta
     1621 ctggcaatga caatggcaat ggcagcgacc atgtttgcag ggtgcggcag cacagcagca
     1681 gacagccaga cagcggatgc acaggggagt gcaggcacgg caacagaggc agcaaagaca
     1741 gaaaaagaca gcggaagtgc tgcgggagaa ggaatcacat tgaccgtgtg ggcgccgttt
     1801 accggatcgg acggcgacgt gctgcgcgag attatcgaca actacaacga gaccaacacg
     1861 gacaacatca cggtacagat tgacattatg gacaacgata ccttgcagac taaatttgcg
     1921 acggcggtat cgacagggac gggaccgtcg tttgtgctgg tgggcattga gtatcttcat
     1981 cagtatgagg agaatggact gattgaggat atcagcgatt tctgggatac gatgggcatt
     2041 gacaagagta atttctatga aaatgtactt gcaaaatcct acatagacga tgcgctctac
     2101 ggcgtaccaa tgcagtacaa tgtgcagtat ctgtattaca acaaggatct tttcacggag
     2161 gcaggtcttg atccggagaa cccgccgacc acgctcgatg aactgcgcga ggcggcgatc
     2221 gcctgcaccg atccgtctaa gaaccagtac ggactgggac ttccgaccga ttacggttat
     2281 tactgcgagt acctgtgggc gaacggcgga gatgtcatca atgaggatgc gacacagaac
     2341 cttttaaatt ccgaagaaaa cattgcaacc ctggagtgga ttcagaagct tgcgacggag
     2401 gacggggtat ccccggaggg actcaccgca gcggatgcgg acacgatgtt ccagtccggc
     2461 cagcttgcaa tgtataccag cggtccatgg aacatcaacg ggctgacaca gcttggtttg
     2521 aattatggaa tcacggcgat cccgggcgga agcgacggcg cgtactcccc ggagggcgga
     2581 tgctcttaca tgctgacgaa aggggccgac gaacagacca aagaggcagt gtataaattt
     2641 atggcatact ggctgtctga tgatgtgtta aaagagtggt cgaaccggaa cggcttcccg
     2701 gtatggtctt actctgtgct tgagaatgcg gacattcaga acaacgagat cttaagtgca
     2761 gtatccaagg catccgagat tggacgtgac tggcatctgg gactggaatg cggaagcatg
     2821 attgacaatg atgtcatgaa accgatgatg gagaaaattt tatccggggc agacgtgacg
     2881 cagagtgtac aggaggcttc ggacgcgctg gacgcggtga tctccgactg atgtctgacg
     2941 gtcactgacg caaaaatagc tgcaagccgt aaaggatgga aattatgaaa gtaaaaaaga
     3001 gacaacaccg tatggagcat caggtgaagg cgtatgcgtt tatcgcgcct tccctgctca
     3061 tacttaccgt atttgtattt ataccgctgg cgggagcgat cgggatcagt ctgatgaata
     3121 ttgatattta tatgaaaaat atctcgttcg ccggaattgc caactacatc aaaatgtttc
     3181 aggatgagcg tgtggcaaat gctacgtttc atacatttta tttcgcactg cttgaggtgc
     3241 ccctccagat gatcgtggcg ttgctgctgc tcatgtttat gctgcgcaat acaaaactgc
     3301 acaagctttt gagaaccgta ttttacctgc cctat
//
LOCUS       DBMDQJ010000227         3332 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10400_length_3332_cov_1.336738, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000227 DBMDQJ010000000
VERSION     DBMDQJ010000227.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3332)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3332)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3332
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10400_length_3332_cov_1.336738"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /gene="coaD"
                     /locus_tag="ACZO9R_05740"
     CDS             complement(<1..449)
                     /gene="coaD"
                     /locus_tag="ACZO9R_05740"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR0912601.1"
                     /translation="MKRAIYPGSFDPLTLGHLDMIKRSAKIVDELVIGVLNNSAKNSL
                     FSLDERVSMIKEMTESMPNVTVASFDGLLVDYMKEINATIIVRGLRAVTDFEYELQIA
                     QTNHVENPEVETIFLTTSLQYSYLSSTIVKEFASYGGDISKFVPARF"
     gene            complement(479..1030)
                     /gene="rsmD"
                     /locus_tag="ACZO9R_05745"
     CDS             complement(479..1030)
                     /gene="rsmD"
                     /locus_tag="ACZO9R_05745"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR0912602.1"
                     /translation="MRIIAGTARSLPLKAPEGMDTRPTTDRIKETLFNIIQDEVPGAY
                     FLDLFAGSGQMGLEAVSRGARYAVFVDNGKKPAACIEENIRFTKFEHQTKLYPTEVLS
                     ALRAMEGKYQFDLIFMDPPYRKGMEQEVLRYLAGSSLLKEDTVLIVEAALDTDFSYLD
                     EYGMTLSRLKMYKTNEHAFIRRK"
     gene            complement(1018..1548)
                     /locus_tag="ACZO9R_05750"
     CDS             complement(1018..1548)
                     /locus_tag="ACZO9R_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HIR0912603.1"
                     /translation="MAAEGQNGSKMLDEMSEYRIFELLKKYHYTLTTAESATGGMIAS
                     TLINVPGISAFFTEGYVTYSNEAKVKMIHVKPETIERYGVVSAETAADMAVSAARTAD
                     TDAALSVTGVAGPDGGTRECPVGLVYIGCCLHGRVVTERHLFAGDRMQVRKSATDAAL
                     HLLARTITEEGTICES"
     gene            complement(1563..2105)
                     /gene="pgsA"
                     /locus_tag="ACZO9R_05755"
     CDS             complement(1563..2105)
                     /gene="pgsA"
                     /locus_tag="ACZO9R_05755"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR0912604.1"
                     /translation="MNLPNKLTIMRVILIPFFVFFLLSPYFPAYGNYIAVAIFIVASL
                     TDMLDGKIARKYNLVTNFGKFMDPLADKLLVCSAMICLIELDRLAAWIVIVIIAREFI
                     ISGFRLVASDNGVVIAASYWGKFKTTFQMLMVIVLILDIQMPFFQILGTVLTYVALIL
                     TVVSLIDYIVKNKDVLKDQK"
     gene            complement(2102..>3332)
                     /gene="rimO"
                     /locus_tag="ACZO9R_05760"
     CDS             complement(2102..>3332)
                     /gene="rimO"
                     /locus_tag="ACZO9R_05760"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR0912605.1"
                     /translation="TDDEYQADIIIINTCCFINDAKEESINTILEMAEHKRDAKLKAL
                     IVTGCLAQRYKEEIRKEIPEVDAILGTNSYEDIVNAITEALGGRFYENFETLEGLPKL
                     TAKRSVTTGGHFAYLKIAEGCNKRCTYCIIPYIRGNYRSVPMEELVAQAKELVAAGAK
                     ELILVAQETTLYGVDLYGEKSLHKLLDALNEIPGLFWIRIMYCYPEEIYEELIDAIAR
                     NEKVCHYLDLPIQHANDTMLKRMGRRTSNADLVRIITHLRERIPDITLRTTLICGFPG
                     ETKEMHEELMQFINDMEFDRLGAFTYSPEEGTPAAEFPDQIDEETKKEWQADVMELQE
                     EIIFDKNEEMKGRELYVFIEGKVSDENAYIGRTYRDAPDVDGYIFINTDEELMTGDIV
                     RARVTGAYEYDLIGELL"
BASE COUNT          757 a          988 c          718 g          869 t
ORIGIN      
        1 atgaatctcg ccggaacgaa cttggagatg tcgcctccgt aggatgcgaa ctccttcaca
       61 atggtggaac tcagatagga atactgcagg ctcgtcgtga gaaaaatcgt ctccacttcc
      121 ggattctcca catgattcgt ctgtgcaatc tgaagctcat attcaaaatc cgtcacagcc
      181 cgaagcccac gcactatgat cgtcgcgttg atctccttca tatagtctac caaaagaccg
      241 tcaaaagatg caaccgtgac attcggcatg gattccgtca tctcttttat catactaaca
      301 cgttcgtcaa gagaaaacaa cgaatttttt gcgctgttat tcagtacgcc gatcaccagt
      361 tcatccacga tttttgcgga tctcttaatc atatccagat gtcccagtgt aagcggatca
      421 aaacttccgg gatatattgc cctcttcata ggtttcttct ccttttcgca tgctccggct
      481 attttctgcg gataaatgcg tgctcatttg tcttgtacat cttgaggcgt gaaagtgtca
      541 ttccatactc atccagatag ctgaaatccg tatctaacgc agcctctacg atcagaaccg
      601 tgtcctcttt taaaagggaa gatcctgcaa gataccgtaa aacctcctgc tccattccct
      661 tgcggtaagg cgggtccatg aaaatcaggt caaactgata tttgccctcc atggcgcgca
      721 gcgcgctcaa cacttccgtc ggatacagct ttgtctgatg ttcaaacttc gtaaaccgga
      781 tattctcctc gatgcaggct gccggctttt tgccattgtc gacaaacacc gcatagcgcg
      841 cgcctctgct gactgcctcc aatcccatct gaccgcttcc cgcaaacaga tccaggaaat
      901 atgccccggg cacttcatcc tgtatgatat taaacagcgt ctccttgatc cggtccgtgg
      961 tcggtctcgt atccattccc tccggtgcct taagcggcag gcttctcgcc gttccagcta
     1021 tgattcgcat atggttccct cctctgtgat cgtgcgggca agtaaatgaa gcgccgcatc
     1081 tgtggcactt ttccgcacct gcatccggtc tcccgcaaac agatgccgtt ccgtgacaac
     1141 ccttccgtgc aggcagcagc cgatgtagac aagcccgacc ggacattccc tggttccgcc
     1201 gtccggtcct gccacgccgg tcacagacag cgccgcatcc gtatccgccg tccgcgccgc
     1261 agataccgcc atatccgcag ccgtctcggc actcacaacg ccgtagcgct caatcgtctc
     1321 cggcttcaca tggatcatct tcaccttcgc ttcgtttgaa taggtgacat atccctccgt
     1381 aaaaaatgcg gagatccccg gcacattgat aagcgttgag gcaatcatcc cgccggtcgc
     1441 ggactctgct gtcgtgagcg tataatgata tttttttaac aattcaaaaa tccgatactc
     1501 cgacatttca tcaagcatct tgctgccgtt ctgtccctct gctgccatct tttcttcctt
     1561 ctctatttct ggtcttttaa gacgtctttg ttcttcacaa tgtaatcgat cagagatacc
     1621 acggtcagaa tcagcgccac atacgtcagc acggtgccga gaatctggaa gaacggcatc
     1681 tggatgtcaa ggatcagaac gatcaccatc agcatctgga acgttgtctt gaactttccc
     1741 cagtagcttg ctgcgatcac aaccccgttg tcggatgcca ccagacggaa accgctgatg
     1801 ataaattctc tcgcaatgat cacaatcaca atccatgccg ccaggcggtc aagctcgatc
     1861 agacagatca tggcagagca caccagaagt ttatctgcca gcgggtccat aaatttgccg
     1921 aaatttgtca caagattgta ctttcttgca atcttgccgt ccagcatatc cgtcaggctc
     1981 gccacgataa agatcgccac tgcaatatag ttgccgtatg ccgggaaata aggcgacagc
     2041 agaaagaata caaaaaacgg aatcaaaatt acacgcataa tggttaattt gtttggtaaa
     2101 ttcataacaa ttctcctatc aaatcatact cgtacgctcc tgtcactctg gcgcgtacaa
     2161 tatcaccggt catcagttcc tcgtccgtgt tgatgaaaat gtagccgtcc acatccggcg
     2221 catcgcggta ggtgcggccg atataggcat tctcatcgct cacttttcct tcgataaaga
     2281 cataaagctc tctgcccttc atctcctcgt tcttgtcgaa gatgatctcc tcctgcagtt
     2341 ccatgacatc cgcctgccac tctttcttcg tctcctcgtc aatctgatcc gggaactccg
     2401 ccgcaggtgt tccctcctcc ggagagtagg tgaaagctcc gagtctgtcg aactccatat
     2461 cattgataaa ctgcatcagc tcctcgtgca tctccttcgt ctctcccgga aatccgcaga
     2521 taagcgttgt ccggagcgtg atgtccggaa tccgctctct caaatgcgtg ataatgcgca
     2581 caagatccgc gttgctggtg cgtctgccca tccgctttaa catggtatcg ttggcgtgct
     2641 ggatcggcag atccaggtag tgacagactt tctcattgcg ggcaatggcg tcaatcagct
     2701 cctcatagat ctcctccgga tagcagtaca taatgcggat ccagaacagt cccggaatct
     2761 catttaacgc atccagcagc ttgtggaggg acttctcccc atagagatcc acgccgtaaa
     2821 gcgtcgtctc ctgcgccact aaaatcagtt ccttcgcgcc tgccgccacc agctccttcg
     2881 cctgtgccac cagctcctcc atcggaacac tgcggtaatt gccgcggata tacggaataa
     2941 tgcagtaggt gcagcgcttg ttgcagccct ccgcaatctt taggtaagca aagtgtccgc
     3001 ccgtcgtcac gctccgcttg gctgtaagct tcggcagtcc ctccagcgtc tcaaaattct
     3061 cgtagaacct gccgcccagc gcctccgtta tggcattcac gatatcttcg taggaattgg
     3121 ttccaaggat cgcatccacc tccggaatct ccttgcggat ctcttccttg tagcgctgcg
     3181 ccaggcatcc ggtcacgatc agcgccttta actttgcatc gcgcttgtgc tccgccatct
     3241 ccaaaatggt gttgatgctc tcttccttcg catcgttgat aaagcagcag gtgttgatga
     3301 tgatgatatc cgcctgatat tcatcatcgg tc
//
LOCUS       DBMDQJ010000228         3331 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10405_length_3331_cov_1.512693, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000228 DBMDQJ010000000
VERSION     DBMDQJ010000228.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10405_length_3331_cov_1.512693"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="ACZO9R_05765"
     CDS             <1..1218
                     /locus_tag="ACZO9R_05765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR0912606.1"
                     /translation="KHMKFTMIRRITALGLILVLAAGIPIQASSASTEKVTEDDASTK
                     SLQEAQDEKAQLEKALKEAQGTIEDLKDSKGDIESKVTELNQQLIDISARITDLENQL
                     TAKSEDIQETKDELAGAKEREAQQYADMKVRIQFMYENGQTSYLEALLSSRNISEFLN
                     SADYIAQIQSYDRQKLTEYQDTVESIVNLEAQLEQEYTDLEALKSTVESNKATVAAMM
                     RQKESELADISGDIEDAQSDADYYAAEIQAQEELIAAIKRAEAEKAAAGVEEHPYTGG
                     AFRWPCPSSTRVTSDYGTRVSPMSGASSNHKGIDIGASAGADIIAAADGTVTAASYSS
                     AAGNYVMIDHGGGLYTVYMHASSLLVSPGQTVSAGDVIAKVGSTGISTGSHLHFGVSL
                     NGSYVSPWSYLGG"
     gene            1318..2637
                     /locus_tag="ACZO9R_05770"
     CDS             1318..2637
                     /locus_tag="ACZO9R_05770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR0912607.1"
                     /translation="MDEQRENMQNGMIENETTGEQKPDGGKERRRRGMGAGILLGICG
                     TLVVGALGIYLTCRFTGSQILITNAAADTDGTTLLDAKTTAKINELSAYIDLYYYEDT
                     DVQDLKDGLYAGLLEGLDDRYSVYYTADEYAQTQVSMTGQYYGIGAGLAQDKDTMQVT
                     ITKVYEGTPSESAGLRNEDVILSVDGTEADTMDVTDLVKLIRGEAGTTVHLEIYRPST
                     GENLSFDVERADVTLPSISSQMLNDTIGYIRIESFEKDTANQFEKALAELEGEGLTSL
                     VVDLRYNGGGLVDSVVQILDDILPEGLIVYTEDKNGHREEYRSSGDTHFDYPMAVLIN
                     QDSASASEIFAGAIKDYNYGTLIGTTTFGKGIVQSLFPLEDGDAIKLTTAKYFTPNGN
                     YIHGVGIDPDIELEYEYLDPDGEQYEIQYDNQVQKAIEVLSEKTQND"
     gene            2656..3171
                     /locus_tag="ACZO9R_05775"
     CDS             2656..3171
                     /locus_tag="ACZO9R_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR0912608.1"
                     /translation="MKLRIYRVITGGLLLLCMVMIFRFSAQPASESEQVSGGVAYRIV
                     ETWSHLTGTEYTQSEMEAYADRIDYPVRKLAHMTEYGILCLLACAFVHGYSGNWKVCG
                     ISAVAVAALYAATDEIHQLFVPGRAGRFSDVCIDTLGAVIWLLLFTALRKLWEKIAKR
                     RGFQYNKNRKK"
     gene            3186..3331
                     /gene="prfB"
                     /locus_tag="ACZO9R_05780"
                     /pseudo
     CDS             3186..3331
                     /gene="prfB"
                     /locus_tag="ACZO9R_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019388.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; frameshifted; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
BASE COUNT          916 a          721 c         1024 g          670 t
ORIGIN      
        1 aaacacatga agtttacaat gatacgaaga ataacagcgc tcggactgat tctggtgctg
       61 gcggcgggca taccgatcca ggcatcgtcg gcttccacag agaaagtgac ggaggacgac
      121 gcttccacaa aatccctgca ggaggcgcag gacgagaaag cccagctgga aaaagcgctc
      181 aaagaggcgc agggcacgat tgaggatctc aaggattcca agggtgatat cgagagcaag
      241 gtaacagaat tgaaccagca gctgatagat atatccgcgc ggatcacgga tcttgagaat
      301 cagctgacgg caaagagcga ggatatccag gagacgaagg acgagcttgc cggagccaaa
      361 gagagggaag cacagcagta cgcggatatg aaagtgcgga tccagttcat gtatgagaac
      421 gggcagacct cttatctgga ggcgctttta tcttccagaa acatttctga atttttaaat
      481 tccgcagatt atatcgcaca gatacagagc tatgacagac aaaagcttac cgagtaccag
      541 gacaccgtgg agtcgattgt gaatctggaa gcccagctgg agcaggagta taccgatctg
      601 gaggcactca agtcgacggt cgagagcaat aaagcgactg ttgccgccat gatgcggcag
      661 aaggagtcgg agcttgcgga catctcggga gatattgaag atgcgcagag cgacgccgac
      721 tattatgcgg cggagattca ggcgcaggaa gaattgattg cggcgatcaa gagagcggaa
      781 gcggagaaag cggcagccgg ggtggaggaa catccgtata ccggaggagc gttccggtgg
      841 ccgtgcccga gcagcacacg ggtgaccagc gattatggaa cgagagtatc cccgatgtcg
      901 ggagcgtcgt cgaaccataa gggaattgat atcggagcat cggcgggagc ggacattatc
      961 gcggcggcgg atggaacggt aacagcggca tcctacagca gtgcggcggg caattatgtg
     1021 atgatcgacc acggcggagg actctacacc gtctacatgc acgcatcatc cctgctggta
     1081 tcgcccggac agaccgtgtc ggcgggagat gtgatcgcaa aagtcggaag taccggcatt
     1141 tcaaccggaa gccatctgca tttcggcgtc tctttaaatg gaagttacgt cagcccgtgg
     1201 agttacctgg gcggctgatg gagaaagtac acgtaggcat tgaccgaaat tccctgtcct
     1261 tggacaggga atttttacat atcttattac ggatagcata cagaaatgag ggaaactatg
     1321 gacgaacaaa gagaaaatat gcaaaacggc atgatagaaa atgagacaac cggagagcag
     1381 aagccggacg gcggaaaaga gcgcagacgc cgtggaatgg gagccggcat ccttcttgga
     1441 atctgcggaa cactggtcgt cggagcgctt ggcatttatc tgacctgccg ctttaccgga
     1501 agtcagatac tcatcaccaa tgcagcggca gatacggacg gaacgaccct gctggatgca
     1561 aagacaacgg caaagatcaa cgaactgtca gcttacattg atctgtatta ttatgaagat
     1621 accgatgttc aggatttgaa ggacggtctg tatgcgggac tgctggaggg actggatgac
     1681 agatattcgg tttactacac ggcggatgag tacgcgcaga cgcaggttag catgacgggt
     1741 cagtattacg gaatcggggc agggcttgcg caggataaag ataccatgca ggtcacaatc
     1801 acaaaggttt acgagggaac accgtctgag agtgcgggac ttcgcaacga agacgtaatt
     1861 ctgtcggtgg acggaaccga ggcggatacg atggatgtca cggatctcgt gaagctgatc
     1921 cgcggggaag ccgggacgac ggtacatctt gagatctacc gtccgtctac cggagagaat
     1981 ctctcgtttg acgtggagcg tgccgatgtg acgctgccga gcatatcttc acagatgtta
     2041 aacgatacga ttggttatat ccggattgaa tcttttgaga aggatacggc aaaccagttt
     2101 gagaaggcgc ttgcggaact ggaaggggaa gggcttacat cgctggtggt cgaccttcgc
     2161 tataacggcg gcggacttgt cgattccgtg gtacagattc ttgacgatat tctgccggaa
     2221 ggtctgatcg tctacacgga ggataagaac ggacaccgcg aggagtaccg ttccagcgga
     2281 gacacgcatt ttgactatcc gatggcggtg ttaatcaatc aggacagcgc cagcgcttcc
     2341 gagatttttg caggagccat caaggattac aactacggaa cgctgatcgg aacgacgaca
     2401 ttcggcaagg gcatcgtgca gagcctgttc ccgttggaag acggagacgc catcaagctg
     2461 acaaccgcga aatattttac gccgaacggc aactatatcc acggtgtcgg aatcgatccg
     2521 gacattgaac tggaatatga gtatctcgac ccggacggag agcagtatga gatccaatac
     2581 gataatcagg tgcagaaggc aatcgaagtg ctgagtgaga agacacagaa cgactgatcg
     2641 gagtgaggag acgggatgaa gcttcggata taccgtgtga taacaggagg acttctgctt
     2701 ctttgtatgg tgatgatttt ccggttttcg gcacagccgg catcggaatc ggagcaggtc
     2761 agcggtgggg ttgcctaccg cattgtggaa acatggagtc atctgacagg aacagaatat
     2821 acacagagcg agatggaagc atatgccgac cggattgatt atccggtgcg caagctggcg
     2881 catatgacag aatatggaat actctgtctg ctggcgtgcg cttttgtgca cggatattcc
     2941 ggtaactgga aagtgtgcgg aatttcggcg gttgcggttg cggcgcttta cgcggcgacc
     3001 gatgagatcc accagctgtt tgtcccgggg cgcgccggac ggttctcgga tgtgtgtatt
     3061 gatacgctgg gagcggtgat ctggctgctg ctttttacag cgctgcgaaa attgtgggaa
     3121 aagattgcga aaagacgggg cttccagtac aataagaata gaaaaaaatg aaagaggtga
     3181 taactgtggt agaacttgat caatttaaag tgaccctgaa cacatacagg gaaccgctgc
     3241 aggaagtgag ggattcactt tgacctggta aataaagaac agcggatcca ggagttagag
     3301 agggagatgg aagctgcgga tttctggaat g
//
LOCUS       DBMDQJ010000229         3327 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10426_length_3327_cov_1.344699, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000229 DBMDQJ010000000
VERSION     DBMDQJ010000229.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10426_length_3327_cov_1.344699"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            158..1336
                     /gene="ftsZ"
                     /locus_tag="ACZO9R_05785"
     CDS             158..1336
                     /gene="ftsZ"
                     /locus_tag="ACZO9R_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR0912609.1"
                     /translation="MTTEADTSAKIIVIGVGGAGNNAVNRMIDENIGGVEFIGINTDK
                     QALTLCKAPTLIQIGEKLTKGLGAGAQPEIGEKAAEESMEELSSAVKGADMVFVTCGM
                     GGGTGTGAAPVVAKIAKEQGILTVGVVTKPFKFEAKQRMVNALSGIDRLKESVDTLIV
                     IPNDKLLEIVDRRTTMPDALKKADEVLQQAVQGITDLINLPALINLDFADVSTVMKDK
                     GMAHIGIGNAKGDDKAIEAVKLAVASPLLETTINGATHVIINISGDISLMDANDAASY
                     VQDLAGDNANIIFGAKYDESMTDEATITVIATGLENPGATSKIMPKLNYATQTPTRPV
                     SGSTLGRTTGTAAPTYQPQGGHGTVTPPLPGIQRPRQPESTVQQMDIKIPDFLKNSRK
                     "
     gene            1534..2310
                     /locus_tag="ACZO9R_05790"
     CDS             1534..2310
                     /locus_tag="ACZO9R_05790"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF015384.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HIR0912610.1"
                     /translation="MYVFYADVFLIQNFLMDFLAVAGANLFLKRCRKMRWLLLGAAVS
                     AVLGLCCILLCRNYLLYCLLTHFLINTLMTVICFGRCDRRQFFENWLAVYLVVLLLGG
                     AFEWLQESGILSKSRGVAIAAAVTVTCGLLRYLGRRRAFGSHVRRAWLGKDARRMALS
                     AYWDSGNQLRDPYTGQPICILSYSLAEKFVDQTKDRIRFVPYRALGAEDGLLAVMDVD
                     EMILQDGRQMRRYEHMAVGIAQKGLLEDKEYDLILHASLG"
     gene            2331..3071
                     /gene="sigE"
                     /locus_tag="ACZO9R_05795"
     CDS             2331..3071
                     /gene="sigE"
                     /locus_tag="ACZO9R_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HIR0912611.1"
                     /translation="MYIKWMVPDYGMRGILNGIGRFLFGENDEVHYIGGAEVLPPPLD
                     AGEESVCIRMLATDAAEEARRKLIEHNLRLVVYIAKKFDNTGVGVEDLISIGTIGLIK
                     AINTFNPDKNIKLATYASRCIENEILMYLRRNSKTRLEVSIDEPLNVDWDGNELLLSD
                     ILGTDEDVIYRDIETDVERSLLMTAIAGLTGRERMIIELRFGLNTAGGEEKTQKEVAD
                     MLGISQSYISRLEKKIMVRLRKEMVKYS"
     gene            3109..>3327
                     /locus_tag="ACZO9R_05800"
     CDS             3109..>3327
                     /locus_tag="ACZO9R_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0912612.1"
                     /translation="MKLTFIGADHEVTGSCHLVEACGKNILVDCGMEQGPDLYENQEI
                     PVASGDIDYILLTHAHIDHSGKLPMLCKQ"
BASE COUNT          900 a          687 c         1002 g          738 t
ORIGIN      
        1 tatggattct gcactgcatt gaaagacgat atacagaaca gacggtgtag gaagaggagc
       61 accggatgat tctctgatag atggtgtgtt tacatccgtt taccacagga catgaattat
      121 gggagagaat gaaggaggaa ctaccttgct agagattatg acgacagaag cagataccag
      181 tgcgaagatt atagtcatcg gagttggagg agctggtaac aatgctgtaa atagaatgat
      241 tgatgaaaat atcggtggag tggagtttat cgggatcaac acggataagc aggcacttac
      301 actttgcaag gcaccgacct taatccagat tggcgagaag ctgacgaagg gacttggagc
      361 cggagcacag ccggagatcg gcgagaaggc agcggaagag agtatggaag agctctcctc
      421 ggcagtgaag ggcgccgaca tggtattcgt tacctgtggt atgggcggcg gtaccggtac
      481 aggcgcagcg ccggttgtag cgaagatcgc aaaagagcag gggattctga cggtcggagt
      541 tgtgacaaag ccgtttaagt tcgaggcaaa acagcgtatg gtcaatgcgc tttccggtat
      601 cgaccgcttg aaagagagcg ttgatacttt aattgtgata ccgaatgaca agcttcttga
      661 gatcgtggac agacgtacca cgatgccgga tgcattgaag aaggcggacg aggtattgca
      721 gcaggcagta cagggtatta cagacttaat caacctgccg gcactcatca acctcgattt
      781 cgcagatgtg tctaccgtca tgaaggacaa gggtatggct cacatcggta tcggtaatgc
      841 caagggagac gacaaggcga tcgaggcagt caagcttgca gttgccagcc cgctgcttga
      901 gacaaccatc aacggtgcta cccacgtcat catcaatatt tccggtgata tttctcttat
      961 ggatgcgaat gatgcggcaa gctatgtaca ggatctcgca ggcgacaatg ccaacatcat
     1021 cttcggtgcg aagtacgatg agtccatgac cgatgaggct acgatcaccg tcattgcgac
     1081 aggacttgag aatccgggcg caacgagcaa gatcatgccg aagttgaatt atgcaacgca
     1141 gacaccgacc agaccggtat ccggttccac actcggaaga accacaggaa ccgctgctcc
     1201 gacctatcag ccgcagggag gacatggtac agtaacaccg cctctgccgg gtatccagag
     1261 accgcgtcag ccggagagca cggtgcagca gatggacatc aagattccgg atttcctgaa
     1321 gaattcacgc aaatagaatt acagtgacat aatatgaact gccatgtgtc agtccgatta
     1381 caggacttgc acatggcagt ttttgtgtta caggaagatt atgtcgaacc gggcagatga
     1441 ctcgccgcat tatagagcgg acttttgaca aaaaaggaga cggcggcatc gtataatagg
     1501 tggcatgtca gagaaaaacc gaggtgaaag gaaatatatg tattttatgc agacgtattt
     1561 ttgatccaga attttctcat ggatttcctt gccgttgcag gggcaaatct ttttttgaag
     1621 agatgcagaa aaatgcggtg gctgctgctt ggggcggctg tttccgcagt gctcgggctg
     1681 tgctgcatcc tgctttgccg caattatctg ctgtactgcc tgctcacgca ttttctcatc
     1741 aacacactga tgacagtcat ctgtttcggg aggtgtgaca ggaggcagtt ctttgagaac
     1801 tggttggcgg tgtatctggt ggttcttctg ctcggcgggg cgtttgaatg gctgcaggag
     1861 agcggtattt tatcaaaaag cagaggagtg gcgattgcgg ctgcagtgac ggtaacgtgc
     1921 ggtcttcttc ggtatcttgg gagaagaaga gcctttggca gccatgtgcg ccgggcatgg
     1981 cttggaaaag atgcgcgccg gatggcgtta agcgcctact gggacagcgg gaaccagtta
     2041 agagatccct ataccgggca gccgatctgt attttgagct atagcctggc ggaaaaattt
     2101 gtggatcaga caaaagacag aatccggttt gtgccgtacc gtgctcttgg agcagaggac
     2161 ggacttcttg cggtgatgga tgtggatgag atgatactgc aggatggcag gcagatgcgg
     2221 cggtatgaac acatggcggt tggaatcgca cagaaggggt tgcttgagga caaagaatat
     2281 gacctgattc tgcatgcatc cctgggatga cagtgaagaa aggagcttac atgtacataa
     2341 aatggatggt cccggattac gggatgcggg gaattttgaa cggaatcgga cgctttttgt
     2401 tcggggagaa tgatgaggtg cactatatcg gcggcgcgga ggtgctgccg ccgccgctgg
     2461 atgcagggga ggagagcgtg tgcatccgga tgcttgctac agatgcggcg gaggaggcgc
     2521 ggagaaaact gatcgaacac aatctgcgtc tggtggtata tatcgcaaaa aagttcgaca
     2581 atacaggagt aggcgtggag gatttgatct cgatcggcac cataggtctc atcaaagcga
     2641 tcaacacatt caatccggat aaaaatatca agctggcaac ctatgcgtcg cgctgcatcg
     2701 aaaatgagat tctgatgtat ctgcgcagaa acagcaagac caggctggag gtgtccatcg
     2761 acgagccgtt aaacgtggac tgggatggaa acgaattgct gctgtcggat attttaggaa
     2821 cggatgagga tgtgatctac cgggatattg agacggatgt ggagcgctcg ctgctcatga
     2881 cagcgattgc cggactgacc ggcagagagc gcatgatcat agaattgcgc ttcgggttga
     2941 acacggcggg aggggaggag aaaacgcaga aagaggtggc ggatatgctg gggatttccc
     3001 agtcctacat atccagactt gagaaaaaaa ttatggtgcg cctgagaaaa gaaatggtga
     3061 agtattcctg aaaaagtggt agaatgaaaa taaaaagggg atacgtgtat gaagcttact
     3121 tttataggag cggaccacga agtgacaggc agctgccacc ttgttgaggc ctgcggaaag
     3181 aacattctgg tagactgcgg tatggagcag gggccagatc tttacgagaa ccaggaaatc
     3241 ccggttgcat cgggagatat cgattatatt ctcctgacgc atgcacatat cgatcactcc
     3301 ggaaagcttc cgatgctctg caaacag
//
LOCUS       DBMDQJ010000230         3321 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10442_length_3321_cov_1.359006, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000230 DBMDQJ010000000
VERSION     DBMDQJ010000230.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3321
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10442_length_3321_cov_1.359006"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="ACZO9R_05805"
     CDS             <1..816
                     /locus_tag="ACZO9R_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR0912613.1"
                     /translation="MIMCGMSAAFSALFGTPMAAAIFSMEVVSVGIMYYAALVPCVIS
                     SLIAHAIATSFGVSPDGFPLGKIPDFTIGSAVEISILAILCALVSILFCVMLHGSDHL
                     YKKFFKNPYLRVFVGGCIVVVLTLLVGDQTYNGTGINIIARCMDGTVRPEAFFLKMLF
                     TAATLGAGYKGGEIVPSLFTGAAFGCLFGNVLGFSPTLCTAVGMSAVFCGVTNCPITS
                     LLISFELFGYDGMPYFLLAIAFSYMLSGYFGLYQSQKIVYSKYKTNYINKKTH"
     gene            complement(855..1001)
                     /locus_tag="ACZO9R_05810"
     CDS             complement(855..1001)
                     /locus_tag="ACZO9R_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912614.1"
                     /translation="MECSRLGKTRIIKDAGRGMVRNTGFYGQICLGQISSGTFGSYFS
                     WYHS"
     gene            complement(1412..1837)
                     /locus_tag="ACZO9R_05815"
     CDS             complement(1412..1837)
                     /locus_tag="ACZO9R_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1284 domain-containing protein"
                     /protein_id="HIR0912615.1"
                     /translation="MADKFRGHHIVCTSLYEGKGYSGAFCENMTAVVERLRKDPDEEL
                     LLVAEPDMICANCPNRTETNECVHNHNRVVNKDRRVIEFFGLEENRVYTYREMCRHAR
                     AAMNMDFFMENCGKCDWRKQGLCKYEDLLAQLDRCIEKE"
     gene            complement(1853..2410)
                     /locus_tag="ACZO9R_05820"
     CDS             complement(1853..2410)
                     /locus_tag="ACZO9R_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR0912616.1"
                     /translation="MKISTKTIVTVGMFTAVLSVLSILTIPMPTGVPVTLQTFAMALC
                     GYVLGWKKGTSSTLIYILLGTVGVPVFAGMTGGPSWLAGYSGGFIWGFIFLTMLCGVG
                     MAQKQIWLRVLLGLAGLAVCHLLGVIQFAIVAATPFPAAFVAVSVPYIVKDVISVVGA
                     YLVAIPIRKALAAGNILEEKTKAAV"
     gene            complement(2564..>3321)
                     /locus_tag="ACZO9R_05825"
     CDS             complement(2564..>3321)
                     /locus_tag="ACZO9R_05825"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR0912617.1"
                     /translation="TMAYTAAALSYMVQNTKKPIVITGAQKPIDLEITDAKSNLIDSF
                     LYAADDRSQGVQIVFDGKVIAGTRAKKVRSKSYNAFSSIDYPSLAVIQDMNIMRYIPM
                     LPYEEEVRFYEKLDENVFLMKLIPGIRPKVLKSIFENYDCIIVESFGVGGIPQSIAEE
                     FYRLCQEHPDKLVVMATQVAHEGSDMTVYEVGHNMKEYCRFLESYDMTLESVIAKVMW
                     MLGNYDISSCDIEEVFYHSINYDLIFGKNRKCN"
BASE COUNT          747 a          937 c          772 g          865 t
ORIGIN      
        1 atgatcatgt gcggtatgag cgctgcgttt tccgcgctgt tcggcactcc gatggcagcc
       61 gccatcttct ccatggaggt ggtgagtgtc ggcatcatgt actatgccgc cctcgtgcca
      121 tgtgtgatct cctcgctgat cgcccacgcg attgccacct cgttcggcgt gagcccggac
      181 ggcttcccgc tcggaaaaat tccggatttt acgatcggct ccgccgttga aatttccatc
      241 ctcgccatct tatgtgcgct ggtgagcatt ctcttctgcg tgatgctgca cggttcagat
      301 catctgtata agaagttttt taaaaatccc tatctgcgcg tcttcgtcgg cggatgcatt
      361 gtcgtcgtgc tgacactgct cgtcggggat cagacctaca acggtacggg catcaacatc
      421 atcgcccgct gcatggacgg taccgtccgc ccggaggctt tcttcttaaa gatgcttttt
      481 accgccgcca cactcggcgc aggttataag ggcggcgaga tcgttccgtc tctctttacc
      541 ggagcggcat tcggctgtct gttcggaaat gttcttggat tttcgccgac cctgtgcaca
      601 gcggttggca tgtccgcagt cttctgcggc gtgacaaact gcccgatcac ctcactgctc
      661 atcagctttg agctgtttgg atatgacggt atgccgtatt tccttctcgc catcgcgttc
      721 agctatatgc tgtctggcta ctttggtctc taccagagcc agaagatcgt ctactccaag
      781 tacaagacta actatatcaa caaaaagacg cactaacagg aatagaaaag cgctgccaca
      841 cgaggcgctg attattatga gtgataccag gaaaaatagg agccaaacgt tccggatgat
      901 atctgtccaa gacagatctg accgtaaaat ccggtatttc taaccatgcc gcgtccggca
      961 tctttgatga tccgtgtctt tccaagtctg gagcactcca cggactgtgt ccgcaggccg
     1021 gcagacaatt ttagggtgtt cgtgtgcccc ttaccgaatt gctgaacaaa acataaccgc
     1081 gcgaagccga tgtttgagtt gccatctgcc ttttttttaa tggcagatgg cagcgagttt
     1141 cggttcgcgc ggttttatat tgtctgtgaa gcaattcgat taggggcgct agaacgcccg
     1201 cccattgtct gcggattgtc ggacacactc tgtggattgt ccagacgtgg caaagacaca
     1261 gaataggatg tcagacgcgg catggaagaa atactcagat tttacttcaa agatctgcct
     1321 tggcagatac attcggaact ttgtgccctc taatctccta tcctgtcact catgctaatc
     1381 agcgcctcgt gtggcagcgc ttttctatct cctattcttt ctcgatacaa cggtcgagct
     1441 gtgcgagcag atcttcgtac ttgcagagcc cctgcttgcg ccagtcgcat tttccacagt
     1501 tctccataaa gaaatccatg ttcatcgccg cgcgtgcgtg tctgcacatc tcgcggtatg
     1561 tatagaccct gttctcctcg agtccgaaga attcgatgac ccggcggtct ttgttcacga
     1621 cacggttgtg gttgtggacg cactcgttcg tctccgtccg gttcggacag ttcgcacaga
     1681 tcatatccgg ttccgccacc agaagaagtt cctcgtccgg atcttttcgc aggcgctcca
     1741 ccaccgccgt catattttca caaaacgcac cgctgtagcc ttttccctca tagagcgaag
     1801 tacagacgat gtgatgtcct ctgaatttgt ctgccatagc aactgtacac tcctaaactg
     1861 ccgcttttgt cttctcttcc agaatatttc ctgctgcaag cgccttgcgg atcggaattg
     1921 ccaccagata cgcgcccaca acggagatca cgtccttcac aatgtaagga acagataccg
     1981 ctacgaaagc agccggaaac ggtgtggcag ccacgatggc gaactgaatg acaccgagca
     2041 gatgacacac agcaagaccg gcaagtccta acagcacgcg gagccagatc tgcttctgtg
     2101 ccatgcccac gccgcagagc attgtcagga aaatgaatcc ccagataaat ccgccgctgt
     2161 agccggcaag ccaggacggt ccgcctgtca ttcccgcaaa caccggaacg cctactgtac
     2221 cgagcaggat atagatcaat gtggatgatg ttcccttttt ccagccgagc acatagccgc
     2281 acagcgccat tgcaaaagtc tgaagtgtca ccggaacgcc ggtcggcatc gggatcgtca
     2341 agatggacaa cacgctaagc actgccgtaa acatacccac tgttacaatt gtctttgttg
     2401 aaatcttcat gattctcctc gtttccgcag gaatcgcata ttttaccgct ctttcctgct
     2461 gtttttcgcg cagaaatccc ctgtgggtct ctctgtctgc aaaaacaatc ataacacttt
     2521 tttttcgatt gtcaacctgt tttatctcgc ggttgacaat cctctaattg catttccggt
     2581 tctttccaaa aatcaggtcg taattgatac tgtgatagaa aacctcctcg atatcacagg
     2641 acgatatatc atagttgcca agcatccaca tcaccttcgc aatcacggac tctaacgtca
     2701 tatcgtagga ctctaagaag cggcaatatt ctttcatgtt gtggccgacc tcgtagaccg
     2761 tcatatcgct tccctcgtgc gccacctgcg tcgccatgac caccagcttg tccggatgct
     2821 cctggcacag ccggtaaaac tcctctgcga tagactgcgg tataccgccc acgccaaagc
     2881 tctccacaat gatacagtca taattttcaa aaatactctt taacaccttg ggacggattc
     2941 ccgggatcag cttcatcagg aaaacatttt catccagctt ctcatagaac cggacttcct
     3001 cctcgtacgg aagcatgggg atatagcgca taatattcat gtcctgtatc acggcgagcg
     3061 acggatagtc aatactggaa aatgcattgt agctcttgga ccgcaccttc ttggcgcggg
     3121 ttccggcgat gacttttcca tcaaacacaa tctgtacccc ctgtgagcgg tcatcggcag
     3181 catataaaaa gctgtcgatc aggttcgatt tcgcgtccgt gatctccagg tcgatcggct
     3241 tctgcgcacc ggtaatcaca atcggctttt tcgtgttctg caccatatag gaaagcgccg
     3301 ctgccgtgta cgccatcgta t
//
LOCUS       DBMDQJ010000231         3304 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10508_length_3304_cov_1.756166, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000231 DBMDQJ010000000
VERSION     DBMDQJ010000231.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10508_length_3304_cov_1.756166"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="ACZO9R_05830"
     CDS             <1..1249
                     /locus_tag="ACZO9R_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HIR0912618.1"
                     /translation="EQNALGTSDATYFTTIEEAGDGLLTGEAVFFVDGFAKAVKIPDD
                     GYPNMGINEADSEKVVRGSNEGFGDSVKQNAALIRKRIRSPQVKVKEKKMGVRSNTNV
                     YLVYMEGIAYPELVAKIEERLEGFEIDGVLDSGVIEQLSEEKWYSPFPQFQTTERPDR
                     AAMSILDGRVVVLSDNSPIALILPTDYNSFIKTSDDYYNRWEIASFERLLRYFASFFA
                     MTLPGLYLAVTNFHTQILPTTLLLSFADARSGVPFPAVIEVIIMELSFELLREAGVRL
                     PGAMGNTIGIVGGLIIGQAAVEANLVSPIVVIVISFTALCSFAVPNEEFATAFRLLKF
                     FFIGICAWLGFFGFLAGLLAVLIHLSHLKSFGVPYLMPYVAADLNDYEDERDFLWRQP
                     LRLLWKRPIYAKKNNRRKLRMKQ"
     gene            1267..>3304
                     /locus_tag="ACZO9R_05835"
     CDS             1267..>3304
                     /locus_tag="ACZO9R_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerAB/ArcD/ProY family transporter"
                     /protein_id="HIR0912619.1"
                     /translation="MFSENNRISGRQMYRLMTYDLLGIGTLLVPQMLAKAAGRDGILS
                     IGIGMAAALMYLWILRRIAPPAQEFYPEYMKRQLGRIGGGIVQAGYLIYLVLLAGYVA
                     YLFADIILRDLLRGESFYLVLSVIMLLVLYGLAGGIEGRARVYEMLFWFLLIPLFLML
                     FAAADEVCTDYWAPLGMSSLKGVAGGAYGVFLNLSFAFFLLFSGTYVRRQETLFAAGK
                     RAILFVGCLHAVLYLVLEGIFGVPALAGMDYPAVTLMSTVKISGGFLKRTDAFMFGVW
                     FFTLYALLNSCVFYGSSIAEKLWKPIRKMPKGKNYMWIFYGSVAVAASVAAICFYRSR
                     AVFDWYERFLWYVGTPFLVLAPVFAAQKKWGRRLLALAVICAVVLLVMTGCAPAELED
                     RDFPIDIAVRDMKDAGLTWYEAEQEGNRMVDYSHLKVLILEQEFVEDEAAVQEWLTFL
                     KEKSEVPRNAYVVVTEDAEALLAQSETLGEAVGDYLEEQFENVSQIKKQAYPTIGSLY
                     QEMDNRQETLFLPYVTVEEEKPVVEQYYVWKRGTPAGMVAGEAARLAFFTQNRMMEYV
                     LPLEEGMLLLSDASNEIAFTKKDGVRKVVVTIHCSGSVQGTGGKENEGKLALSAEDYM
                     NRMAANVRQEKQVDLTGSYRKLGGAARGWYEEYQERGENYEEEVEVVYRVKINWI"
BASE COUNT          731 a          692 c         1084 g          797 t
ORIGIN      
        1 ggaacagaat gccctcggaa cctccgatgc gacctatttt acgacgatcg aggaggcggg
       61 ggacggtctt ttgaccggag aagccgtttt tttcgtcgac ggttttgcga aagcggtcaa
      121 gattccggat gacggttatc cgaatatggg gatcaacgag gcggactcgg aaaaagtggt
      181 gcgtggttct aacgagggat tcggggattc ggtaaaacag aatgcggcgt tgatccgcaa
      241 gagaatccgg tcgccacagg tcaaggtaaa agagaaaaaa atgggcgtgc gctcaaacac
      301 caacgtctat ctggtctata tggaagggat cgcctaccca gagttggtgg caaagattga
      361 ggagcggctg gagggatttg agattgacgg ggtactcgac agcggagtca ttgaacagct
      421 gtcggaggaa aaatggtatt ccccgtttcc acagtttcag acgacggaac ggccggaccg
      481 ggcggcaatg tccatccttg acggcagagt ggttgtgctg tcggacaatt cgccgatcgc
      541 gctcattctg ccgaccgatt acaattcctt tatcaagacg agcgacgatt actataaccg
      601 ctgggagatc gcaagctttg agcgcctttt gcgctatttt gcatcctttt ttgccatgac
      661 attgccggga ctctatctgg cggtcaccaa tttccacaca cagattctgc cgaccacgct
      721 cctgctgtcc tttgcggacg ccaggagcgg cgtgccgttt ccggcggtga ttgaggtgat
      781 tatcatggag ctttcgtttg aactgctgcg ggaggcggga gtgcggctgc ccggagcgat
      841 ggggaatacg attggaattg tggggggact gatcatcggg caggcagccg tggaggcaaa
      901 tctggtgagt ccgattgtcg tgattgtgat ctcgtttacg gcgctgtgtt cgttcgccgt
      961 accgaacgag gagtttgcaa cggcattccg gctgctcaag ttctttttta tcggaatctg
     1021 cgcatggctt ggttttttcg ggtttctggc agggctgctt gccgtgctga tccatctgtc
     1081 gcacttaaaa agctttggcg tgccgtacct gatgccgtat gtggcggcag atctgaacga
     1141 ttatgaagat gaacgcgatt tcctgtggcg gcagccgctt cggctgctct ggaaacgccc
     1201 gatctacgcg aagaagaata accgcagaaa gctgcggatg aaacagtgac agagagggaa
     1261 cggactatgt tttcagaaaa taacaggata tcgggacgcc agatgtatcg gctgatgacg
     1321 tatgatctgc ttggcatcgg tacacttctc gtcccgcaga tgctcgcgaa ggcggcgggg
     1381 agagacggca ttctgtcgat cggaatcggg atggcggcag cacttatgta tctttggata
     1441 ttgcgcagga tagcgccgcc cgcacaggaa ttttatccgg aatatatgaa gcggcagctg
     1501 ggaagaatcg ggggaggaat cgtacaggca ggttatctga tctacctggt gctgcttgcg
     1561 gggtatgtgg catatctgtt tgcagacatt attctccggg atcttctgcg cggagaatcg
     1621 ttttatctgg tcctttcggt tatcatgctg ctggtgctgt atggacttgc ggggggaatc
     1681 gagggacggg cgagagtcta tgagatgctg ttctggtttc ttttgatccc gctgtttctc
     1741 atgctctttg ctgcggcgga tgaagtgtgc accgattact gggcgccgct tggaatgagc
     1801 agcttaaagg gtgtggcagg cggagcttac ggggtgtttt tgaatctttc ctttgcattt
     1861 ttccttcttt tttcgggaac atatgtcagg cggcaggaaa cattgtttgc agcgggaaag
     1921 agggcaatcc tatttgtggg atgtctccat gcggtgctct atctggttct ggagggaatt
     1981 ttcggggtac cggcgcttgc ggggatggat tatccggcag tcacgctgat gagcacggta
     2041 aaaatatccg gcggcttttt gaaaagaaca gacgcgttta tgttcggcgt gtggttcttt
     2101 acgctgtatg cacttttaaa cagctgcgtc ttttacggaa gcagcatagc agagaaatta
     2161 tggaaaccta ttcgcaaaat gccaaaaggg aaaaattaca tgtggatttt ctatggatct
     2221 gtggcggtgg cagcttctgt ggcagcgatc tgtttttacc ggagccgggc ggtctttgac
     2281 tggtacgagc ggttcttgtg gtatgtcggt acgccgtttc tggtgcttgc ccccgtcttt
     2341 gccgcacaga aaaaatgggg aaggaggctg cttgcgcttg cggtgatctg tgcagttgtc
     2401 ctgcttgtga tgacaggctg tgcccccgcg gagctggagg atcgcgattt tccgattgat
     2461 atcgcggtgc gggacatgaa ggacgccggg ctgacgtggt atgaagcgga gcaggaggga
     2521 aaccggatgg tggattacag ccatctgaag gttctgatcc tggaacagga atttgtggag
     2581 gacgaggcgg cggtgcagga gtggctgaca ttcttaaagg agaagagcga ggtgccgcgc
     2641 aacgcctatg tggtggtcac ggaggatgcc gaagcactgc tggcgcagag tgagacgctg
     2701 ggagaagcgg tcggggatta tctggaggaa cagtttgaaa atgtgtccca gatcaaaaag
     2761 caggcatatc ccacaatcgg atcgctgtat caggagatgg acaatagaca ggagacgcta
     2821 tttttgccgt atgttactgt ggaggaggaa aagccggtgg tggagcagta ttatgtgtgg
     2881 aagcgcggga cgccggcggg catggttgcc ggggaggcag cgagactcgc ctttttcacg
     2941 caaaaccgca tgatggagta tgtcttgccg ttagaggagg gaatgctgct gctgtcggat
     3001 gcctcaaatg agatcgcgtt tacgaaaaag gatggtgtgc ggaaggttgt ggtgacgatt
     3061 cactgcagcg ggagcgtgca gggaaccgga ggaaaggaga atgaaggaaa gcttgcactt
     3121 tcagcggaag attatatgaa ccggatggcg gcaaatgtgc ggcaggagaa gcaggtggat
     3181 ctgacaggca gctaccggaa gctcggaggc gcggcgcgcg gctggtatga ggagtaccag
     3241 gagcgcgggg agaactacga agaagaggtg gaggttgtct accgggtaaa aataaactgg
     3301 attc
//
LOCUS       DBMDQJ010000232         3304 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10511_length_3304_cov_1.121449, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000232 DBMDQJ010000000
VERSION     DBMDQJ010000232.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3304)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3304)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3304
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10511_length_3304_cov_1.121449"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(303..848)
                     /locus_tag="ACZO9R_05840"
     CDS             complement(303..848)
                     /locus_tag="ACZO9R_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing response regulator"
                     /protein_id="HIR0912620.1"
                     /translation="MTNIIVAFPKQDNARNIKKILMQNGHHVDAVCSTGAQVLQSAGE
                     LGGGIVVCGYRFVDMMYTELHEYLPVQFEMLLVASPANCGNRDVENLVCLATPLKVNE
                     LLDTVEMMEYTITRRRKKLKQIPKERTKEEQELINEAKALLMERNNLSEEEAHRYIQK
                     RSMDNGTGLTETAQMILSLLA"
     gene            complement(873..2204)
                     /gene="glnA"
                     /locus_tag="ACZO9R_05845"
     CDS             complement(873..2204)
                     /gene="glnA"
                     /locus_tag="ACZO9R_05845"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HIR0912621.1"
                     /translation="MSKYTKQDIIRIVEEEDVEFIRLQFTDMFGTLKNVAITSSQLEK
                     ALNNECMFDGSSIEGFVRIEESDMYLYPDLDTFVIFPWRPQQGKVARIICDIYTADRK
                     PFEGDPRYALKKAVADAESMRYRFDVGPECEFFLFNQDEDGQPTTMSTERAGYFDLGP
                     VDLGENARRDMVLTLEDMGYEIEASHHEVAPAQHEIDFKYDEALKTADNIMTFKLAVK
                     TIAKRHGMFASFMPKPKYGVNGSGMHVNMSLSKEGRNIFDDPDGENGLSREAYWFIGG
                     IMNHMRAMTAITNPLVNSYKRLVPGYEAPIYIAWSMTNRSPLIRIPVSRGSRTRVELR
                     CPDSAANPYLTLAVCLEAGLDGIRRQIDPPAAVTENIFEMRLSQIKKQGIESLPADLG
                     EAVEAFKADPYIREVLGEHISEKYSEAKMAEWADYRAQVTGWEIDNYLYKI"
     gene            complement(2322..3095)
                     /gene="hisA"
                     /locus_tag="ACZO9R_05850"
     CDS             complement(2322..3095)
                     /gene="hisA"
                     /locus_tag="ACZO9R_05850"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HIR0912622.1"
                     /translation="MEFRPCIDIHNGKVKQIVGSSLADEGDRAVENFVAGADAAYYAH
                     LYKEAGLRGGHIILLNSRDSAYYEATKAQALQALAEYPGGLQIGGGIDAANAKQFLDA
                     GASHVIVTSYVFRDGKIDYERLATLRKNVGKEHLVLDLSCAKRADGYYIVTDRWQKDT
                     RERVTTELLERLGSECDEFLVHAVDVEGKANGIERELASLLGSWGRIPVTYAGGVRDF
                     DDLALLRELGCDRLNVTIGSALDLFGGSMPFEKVTEYCR"
BASE COUNT          770 a          981 c          603 g          950 t
ORIGIN      
        1 tttctgataa ttatcattga tcttgtacat gtctctctcc ttattccttc catacagaat
       61 tcgttctctt tcaaaatgtc gccaacgaca caacgcattg ccactctctg tcatccatta
      121 tctcatactt aaatccaatc cgcaagcact ttttgattcc tggtaaacaa aattctcctg
      181 tctgcgactt ttcctattcg cacaggtatg catccccgcg gagcgttttt gtataacagg
      241 aacgatattt cctattacaa aaaaaggacc gccgcatctg cggcaatcct ctctctcact
      301 catcaggcaa gcaaacttaa aatcatctgg gctgtttctg tcagaccggt tccattatcc
      361 atgctgcgtt tctgtatgta ccgatgcgcc tcttcctccg acagattgtt acgttccatc
      421 agaagtgctt ttgcctcatt tatcagctcc tgttcctcct tcgtgcgctc cttcggaatc
      481 tgcttcaact tttttctgcg cctggtgatg gtatattcca tcatctccac cgtatctaac
      541 agctcgttta ccttaagcgg cgtggcaagg cagaccagat tctccacatc ccggtttcca
      601 cagtttgcag gagacgcaac caacagcatt tcaaattgta ccggcagata ttcgtgcagt
      661 tctgtgtaca tcatatccac aaaacgatac ccgcacacga caatcccgcc gccaagttca
      721 ccggcgctct gtaacacctg cgctccggtg ctgcagactg catcgacatg atgaccgttc
      781 tgcatcagta tttttttgat attgcgggca ttatcctgtt tgggaaatgc aacaataata
      841 tttgtcaatt ctatcccctt tgcatccagg ctctatattt tataaaggta attgtcaatt
      901 tcccagcctg tcacctgcgc acgataatct gcccactctg ccatctttgc ctcgctgtac
      961 ttctcgctga tgtgttcgcc aagcacttcc cgaatgtaag gatccgcctt aaatgcttcg
     1021 accgcttctc ccagatccgc cggaagtgat tcgattccct gcttcttaat ctgcgacagc
     1081 cgcatctcga atatattctc agtcacggca gccggcggat cgatctgccg gcggattcca
     1141 tccagtccgg cttccagaca caccgcgagc gtaagatacg ggtttgccgc cgaatccggg
     1201 cagcgcagtt ccactctggt tctgctgccg cgcgataccg gaatccggat cagcggactg
     1261 cggtttgtca tggaccatgc gatataaatc ggcgcctcat atcccgggac aagacgctta
     1321 taggaattga caagcggatt ggtaattgca gtcatggctc tcatatggtt cataatgcca
     1381 ccgataaacc agtaagcttc cctgctcagt ccgttctccc cgtccggatc gtcaaaaatg
     1441 ttccgacctt ccttggaaag cgacatattc acatgcattc cggaaccatt caccccgtac
     1501 ttcggctttg gcataaagct tgcaaacatg ccgtgccgct ttgcaatggt ctttaccgca
     1561 agcttgaatg tcatgatatt gtccgctgtt tttaacgcct cgtcatattt aaagtcaatc
     1621 tcatgctgcg ccggtgctac ctcatgatgg gacgcctcta tttcatagcc catatcctct
     1681 aatgtcagca ccatatctct tcttgcattc tccccgagat caaccggtcc cagatcaaaa
     1741 tatccggcac gctccgtgct catcgttgtc ggctgtccat cctcatcctg attaaacagg
     1801 aaaaattcac attccggacc gacatcaaaa cggtatcgca tagattccgc atctgccacc
     1861 gccttcttta aagcataccg cggatctccc tcaaacggtt tgcggtctgc ggtataaata
     1921 tcacagatga ttctggcaac ttttccctgc tgtggccgcc acggaaagat tacaaacgta
     1981 tccagatccg gatacagata catgtccgac tcttcaattc tcacaaatcc ctcaatggag
     2041 gacccgtcaa acatacattc attgttcaat gctttctcaa gctggctgga cgtgattgcc
     2101 acattcttga gtgtcccgaa catatcggta aactggagcc tgatgaactc cacatcctct
     2161 tcctccacga ttcggataat atcctgcttt gtgtacttgc tcattttctt ctcctccatc
     2221 ttccactgcc atgatctttc ctcactgccg tgatcttttc tcactgtcaa gttccttcct
     2281 cactgccgtg atcttttctc actgtcgcga ttcttctcct gctatctgca atattctgta
     2341 actttttcaa aaggcatcga tcctccgaac aaatccagtg cacttccgat cgtcacattc
     2401 agcctgtcac atccaagctc tctcaaaagt gcaaggtcat caaagtcccg cactccgccg
     2461 gcataggtaa ccggtatcct gccccaggag ccaagcagac ttgcaagttc tctctcaata
     2521 ccgtttgcct taccctccac atcgaccgca tgtactaaaa actcgtcaca ctcactgccg
     2581 aggcgttcca aaagttctgt tgtcacacgt tccctcgtat ctttctgcca gcgatcggtc
     2641 acaatataat aaccatccgc ccgctttgca cagcttagat ccagaaccag atgttctttt
     2701 ccgacatttt tcctcaatgt tgcaagccgt tcatagtcaa tttttccgtc acgaaacaca
     2761 taggaggtga caatcacatg acttgcgccc gcatccagaa actgctttgc gttcgcagca
     2821 tcaatgcctc ctccgatctg aagtccgccc ggatactccg caagcgcctg cagggcctgt
     2881 gcctttgtcg cctcatagta cgcactgtct cgcgaattca acagaatgat atgtccgccg
     2941 cgaagtcccg cttccttata tagatgtgca taatacgcag catccgctcc ggcgacaaaa
     3001 ttctccactg cccggtctcc ctcatccgca agactgctgc cgacaatctg cttgaccttg
     3061 ccgttatgta tatcaataca gggacgaaac tccatactgc ctccttatac actgctctcg
     3121 taatataaat ttaggcaacg taccccctgt aacagtggct ccgtcgccca ctgccatcat
     3181 agcacacatc tgtccgtttg tgaatatttt tatatattag tctaacttat caatatattt
     3241 ttttatcaac tgacacttat actacattga cacacacatt tttttttgct attatagaga
     3301 aaat
//
LOCUS       DBMDQJ010000233         3302 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10514_length_3302_cov_1.536707, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000233 DBMDQJ010000000
VERSION     DBMDQJ010000233.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10514_length_3302_cov_1.536707"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="ACZO9R_05855"
     CDS             complement(<1..501)
                     /locus_tag="ACZO9R_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR0912623.1"
                     /translation="MEQYVIKGGNPLVGEVEIGGAKNAALAILSAAVMTDETVTIENV
                     PNVRDTNVLLNAIEEIGARVERLDAHTVRINGSFIRDFNVDNEFIRKIRASYYLIGAL
                     LGKYKRAEVALPGGCNIGGRPIDLHMKGFRALGAEVDIKYGLVRAYAEKLTGTHIYLD
                     KVSVGAT"
     gene            complement(672..2228)
                     /locus_tag="ACZO9R_05860"
     CDS             complement(672..2228)
                     /locus_tag="ACZO9R_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR0912624.1"
                     /translation="MKKQTFTKQAVWQAAAVGVLAVGGVLFCLSGQIGIGQTYRQVRV
                     DDRIIGYAPENTDMDDIVRQARQELTQKTGERISGEVVWSSEECRKPFQKLLSENELK
                     EAVENILSEKNAEEKQRVYTVAIDEYRANFATMDEVESFLEKVKASADAADEYGAVIA
                     DQGSHVSGILTASLARRDAGAAADEKAPEETQVPLPQAGISAYVICTLEEAFADPKAT
                     YETGILDMEFVERVNVFENYVDADQIADVDEQVAEVTKEKESNKIYVVESGDCLSVIA
                     MDHDTTVSSIVALNGLDSADAMIRDGQELIIAVPEPDLQLRVTKGEVYEEDYMEEPII
                     IDNDSWYTTKEVVQQEGTTGHRERNDVVVYENGVETSREMIHQNVMVASQAAVIERGT
                     IIPPTYIKPISGGRYTSGFGRRWGRMHKGVDWACPIGTTVYASCAGTVISASYNGGYG
                     NNVVISHADGRLTRYAHNSKLLVKAGQHVEQGEPIALSGSTGRSTGPHVHFEIYINGS
                     AVDPLKYISN"
     gene            complement(2225..2731)
                     /locus_tag="ACZO9R_05865"
     CDS             complement(2225..2731)
                     /locus_tag="ACZO9R_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR0912625.1"
                     /translation="MKKNIILIGMPGVGKSTVGVVLAKVMGYQFIDADLVIQEKEGKL
                     LREIIAEVGPEGFLKVENRINSGIEAEHAIIATGGSVVYGAEAMEHLGRIGTVVYLKL
                     PYRELERRLSDIKGRGVVLRDGQTLHDLYEERVPLYEKYADVIVDEHHLNVEQTIEKI
                     MELKERFQ"
     gene            complement(2747..>3302)
                     /locus_tag="ACZO9R_05870"
     CDS             complement(2747..>3302)
                     /locus_tag="ACZO9R_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR0912626.1"
                     /translation="QFEVVIPIAGEHNVYNALAAVCVARRLGLDAEEMKLGIEHAHTI
                     GGRSNLIRKNGITIIDDCYNANPVSMMASIDVLSQAKGRKIAVLGDMGELGSEEKKLH
                     GMVGTHFAGKKIDALFVTGTLAGEIAEEARRVAPETRVYAEPDKEALEEALLEYRRPG
                     DTILVKASHFMGFSEIVKKLEQPQ"
BASE COUNT          701 a         1049 c          640 g          912 t
ORIGIN      
        1 ggttgccccg acagacacct tatccagata aatgtgggtg ccggttaact tttctgcata
       61 agcccgcacc agaccatact tgatatccac ctctgcgccc agagccctaa agcccttcat
      121 atgcagatcg atcggccgtc cgccgatgtt gcatccgccc ggaagcgcca cttccgcccg
      181 cttatatttg ccgagcagtg cgccgatcag ataataagat gcacgtattt ttcggataaa
      241 ttcattgtca acattaaaat cccggataaa agatccattg atccttaccg tgtgagcatc
      301 cagccgctct acccgcgctc caatctcctc gatggcattg agtaaaacgt tggtatccct
      361 gacattaggt acattttcta ttgtaacggt ctcatccgtc ataactgcag cagataagat
      421 cgcaagcgcc gcattcttag cgcctccgat ttccacctct cctaccagcg gatttccacc
      481 tttgatcaca tactgttcca tatcacacct ctatgaacgc tttatacaaa cacgtaatta
      541 tactagcgct tacatgattt tgtcaagtaa ttcttaagtt cttcgttcat caaccatata
      601 gtgtacatta taccataaat ttcatccatt tgtacagcat acaactattt ccagtctgtg
      661 tcctcttccc atcagttgct gatgtatttg agcggatcca ctgctgaacc gttgatatag
      721 atctcaaaat gaacatgcgg tcctgtagaa cgtcccgtac ttccacttaa cgcgatcggc
      781 tctccctgct caacatgctg tccagccttc accagaagct tactgttgtg tgcatatctc
      841 gtcagtctgc catccgcatg gctgatgacc acgttattgc cgtagccacc attataggac
      901 gcagagatta ccgttccggc acaggatgcg tacaccgtcg tcccgatcgg gcatgcccag
      961 tcaacgccct tgtgcatacg tccccagcgt ctgccgaatc ctgacgtata tcttcctccg
     1021 gaaatcggtt tgatataggt cggcggaatg atggtaccgc gctcaatgac tgccgcctgt
     1081 gacgccacca ttacgttctg atgtatcatc tcgcggcttg tttccacacc gttctcatag
     1141 accacaacgt cattgcgctc ccggtgtccc gtcgttccct cctgctgtac aacctctttt
     1201 gtcgtatacc aggaatcgtt gtctatgatg atcggctctt ccatgtagtc ctcctcatag
     1261 acctctcctt ttgttacccg aagctgcaga tccggctccg gcaccgcaat gatgagttcc
     1321 tgaccgtccc ttatcatcgc atccgcgctg tccagcccgt ttaacgccac gatcgacgac
     1381 actgtcgtat catgatccat cgcaatcacg gagagacagt ctccgctctc cactacatat
     1441 attttattcg attctttctc ttttgttact tcggcaacct gttcatccac atctgcaatc
     1501 tgatcggcat cgacatagtt ctcgaacaca ttcacccgct ccacaaattc catatcgagg
     1561 atgcctgtct catacgtggc ttttggatct gcaaatgcct cctccagcgt acagatcaca
     1621 taggcgctga ttcctgcctg cggcagagga acctgtgtct cctccggcgc tttttcatcc
     1681 gctgcagccc ctgcgtccct tctggcaaga gacgctgtca gaattccgct cacatgggag
     1741 ccctgatccg caatgaccgc cccgtattca tccgccgcat ccgcagaagc cttcactttt
     1801 tccagaaaag actccacctc gtccatcgtc gcaaagttgg cgcggtattc atcgatcgcc
     1861 actgtataga cgcgctgctt ttcctctgca tttttctcac ttagtatgtt ctctacggct
     1921 tcttttaatt cattctccga caacaacttc tggaacggtt ttctacactc ctcactgctc
     1981 catacgactt cgccgctgat tctctccccg gtcttttgcg tcagttcctg tctcgcctga
     2041 cggacgatgt catccatatc agtgttctct ggcgcatacc cgatgatgcg gtcatccacg
     2101 cgcacctgcc ggtatgtctg tccgattcca atctgcccgg aaaggcaaaa aaggactccg
     2161 ccgaccgcca ggactcctac cgcagctgcc tgccagactg cctgttttgt aaacgtttgt
     2221 ttcttcattg aaagcgttcc ttcaattcca taattttctc aatcgtctgt tccacgttca
     2281 aatgatgttc atccacaatc acatccgcat atttctcgta caaaggcaca cgttcctcat
     2341 agagatcatg aagcgtctgt ccgtctctta agacaacccc tcttcccttg atgtccgaga
     2401 gcctgcgctc caattcccgg tacggaagtt tcagataaac caccgtaccg atccggccaa
     2461 gatgttccat cgcttccgct ccgtacacca cgctcccgcc ggttgcaata atggcatgtt
     2521 ccgcctcgat cccgctgttg atgcggttct ccaccttaag gaatccctcc ggtccgactt
     2581 cagcgataat ctcacgcaga agctttcctt ccttttcctg tatcaccaga tcggcatcaa
     2641 tgaactggta tcccatcact tttgcaagaa ccacgccaac ggtactcttt ccgacaccgg
     2701 gcattccaat cagtatgata ttctttttca tgtgtacctt ccttattcac tgtggctgtt
     2761 ccaatttttt cacaatctcc gaaaatccca taaaatggga cgcctttacc agaatggtat
     2821 cccccggtct gcggtactcc agcagcgcct cctccagcgc ctctttgtcc ggttccgcat
     2881 agaccctggt ctccggcgct acacgccgcg cctcctccgc aatctcccct gcaagtgttc
     2941 cggtcacaaa cagcgcatcg atcttcttcc ccgcaaagtg tgtgccgacc attccgtgca
     3001 gcttcttttc ctcactgccg agttctccca tatcgccgag cacggcaatc tttcgtccct
     3061 tcgcctggga cagcacgtct atggaagcca tcatggacac cggattcgca ttgtagcagt
     3121 cgtcgataat tgtgatgccg tttttccgga tcagattact ccgcccgcca atcgtgtgcg
     3181 catgctcaat tccaagcttc atctcctccg catcaagtcc cagccgtctt gccacgcaga
     3241 ctgctgcaag cgcgttgtat acattatgct caccagcaat cgggatcacc acctcaaact
     3301 gc
//
LOCUS       DBMDQJ010000234         3283 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10593_length_3283_cov_1.270899, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000234 DBMDQJ010000000
VERSION     DBMDQJ010000234.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10593_length_3283_cov_1.270899"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            248..1786
                     /locus_tag="ACZO9R_05875"
     CDS             248..1786
                     /locus_tag="ACZO9R_05875"
                     /inference="COORDINATES: protein motif:HMM:NF025885.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR0912627.1"
                     /translation="MKLRDLSRRIREEDRPDISFALQGGMELSDINTDWLADFPETVY
                     IANSQSVIFGKVQKETLLYLIARQREFQFRQILDSMNDGVIAVDETGRIFYANPAYVS
                     ILGVPLRRIMGKRIQSIEPTSLLSQTVEKKIPLTSEKQIIPSIKKYVSLRAVPLWDGE
                     TFLGAVSIFQDVTKLHRLGQEVQQMSDIVDEYSHRIRSQEIAEKMRITSYNKAFQTLI
                     QKVATVALTDVPMLIQGESGTGKSAMAYYLHQCSGRREKPFLVMNCAAIPQNMMESEL
                     FGDNERPGKLMLADGGTLFLDEVEELTLPAQSRLLHYIGQNKDNNIGERTAALPDTRI
                     IASAGQALEPLVDTKHFRRELFFQLSTIAVTLPPLRERRDDIIPLANQFLSDYNEKYH
                     RDVMFSSQVYQDLQKYDWPGNLQELKSYVERAVILSDNGLPIIELSCDQKDAQAEGAE
                     DDWKLRDRPLAEQMQEFEQRVIRETLKACGGNRTEAMRRLGLSRRTFYRKCEKLEGPP
                     SAKK"
     gene            1963..>3283
                     /locus_tag="ACZO9R_05880"
     CDS             1963..>3283
                     /locus_tag="ACZO9R_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009255204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR0912628.1"
                     /translation="MKKVSIGGGQGFWGDSPDAAIHMIRHGNLNYMACDYLAELTLSI
                     MQRQKMKNPAAGYARDFISLMKEIGKEAYEKKIGIITNAGGMNITGAVDALKAVAEEQ
                     EMHGYKIGYVTGDDLLDQIPQMIADGCEFKNMDDVGDFNEIKDKLVNANVYYGREPIM
                     ECLAQGANTVLTGRAADSAMFLAPLAYEFGWKDNDLDNLARGIMAGHLLECGGQGSGG
                     NFQYDWRSVPDMDQLGFPIAELTEEDFHITKAPDCGGLISEQSCKEQFLYEVHDPANY
                     ITPDVTVDISHATLTQDGNNRVRVGNVKGKARPDQLKLCLGYHAGYKVVTYLSFAWPD
                     AYEKAQYAAEILMKKMKRKGMQYEDLRIDYVGLNALHLDVAEIDDDLIKRTNEVVLRI
                     AIRTKEKRDAQLIIPEISPLQLNGPPGASFFGGRAHVTDVIGLWPTLI"
BASE COUNT          847 a          768 c          912 g          756 t
ORIGIN      
        1 ctcgaacctg cgacctccgg gttatgaggc agtctcaagc acaaatcgga gccatttcgg
       61 ctccgatttg tgctttttac caccgttcgt tcggcggatt ttccattgtt tccgtccaag
      121 cctgcccgct tttttcggat tctgggtcaa aactgggtca agagttgaaa aaccaataag
      181 gccttcttgc accggaaaga caactgtgtc ataatgaagc aaaaaatgga ggaggaagtg
      241 gggcattatg aagctacggg atctttccag acgtattcgg gaagaagatc gaccggatat
      301 ttcctttgca ctccagggag gtatggagct ttctgatata aataccgact ggctggcaga
      361 ttttccggag acagtgtata ttgccaatag tcagtccgtc atttttggga aagtacagaa
      421 agaaacgctt ttatatctga tcgcccgtca gcgggaattt cagtttcgac agatcctgga
      481 tagtatgaat gacggcgtga tcgccgtaga cgagaccggg cggatcttct atgccaatcc
      541 agcttatgtt tccattttgg gcgtacctct gcggcggatc atggggaagc ggatccaaag
      601 catcgagccg acatcgcttc tgagccaaac ggtagaaaag aagatccctc tgaccagtga
      661 aaaacagatc attccctcta tcaaaaagta tgtttctcta cgggcagttc ctttgtggga
      721 tggagaaaca tttttgggtg cggtctcgat ctttcaagat gtgaccaagc tccacaggtt
      781 gggccaggag gtccagcaga tgtccgatat tgtagatgag tattcacatc gtattcgcag
      841 tcaagagatc gcggaaaaaa tgcggatcac atcatataac aaagcctttc agacgcttat
      901 tcagaaggtt gcaactgtgg ccctgacgga tgtaccaatg ctgatccagg gagagagtgg
      961 caccgggaag agtgccatgg catactactt gcatcagtgc agtggccgca gggaaaagcc
     1021 atttttggtg atgaactgcg cagccattcc tcaaaatatg atggagagtg agctttttgg
     1081 cgataatgag cgaccgggta agctgatgct ggcggatggc ggaacgctgt tcctggacga
     1141 ggtggaagaa ctgacgctcc cggcgcagtc ccggctcctg cactacattg ggcaaaacaa
     1201 agacaacaat atcggcgaac gcacggctgc tttaccagat acgcggatta ttgcatccgc
     1261 tggccaggcg ttggaaccac tggtggatac aaaacacttc cgtcgggaat tgttttttca
     1321 actgagtacc attgcagtca ccctgccgcc gctgcgagag cggcgggatg acattatccc
     1381 gctggcgaat caattccttt cggactacaa tgaaaaatac catcgtgacg tgatgttttc
     1441 ctcccaggtc tatcaagatc tgcaaaaata cgattggccg ggaaatttac aggagctgaa
     1501 gagctatgta gaacgagcgg tcattttgtc ggataacggc ctgccgatca ttgaactgtc
     1561 ctgtgaccag aaagatgcac aggcagaggg agcggaggat gactggaagc tcagggatcg
     1621 tcccttggcg gagcagatgc aggagtttga acagcgggtg atccgagaga ccttgaaggc
     1681 atgtggcggc aaccgcacag aggccatgcg gcgtttaggc ttgagcagaa gaacttttta
     1741 ccggaagtgt gaaaagttgg aaggaccgcc gagtgccaaa aagtaaacag tgccacaaag
     1801 taaacacaca atagtgccaa ctttggcacc attgtgtgtt tcttttttca aaagtgctgc
     1861 ctggattttt ttatattcta caaaagacat cgaaaatact ctttgctaca atgatatcgt
     1921 caaaagaatg agaccgagca cagtaaaaag gagatgttaa gagtgaaaaa agtatccatt
     1981 ggaggcggac agggtttctg gggcgacagt cctgacgctg ctatccacat gattcgtcac
     2041 ggcaacctga attacatggc ttgcgactat ttggcggagc tgactttgtc gatcatgcag
     2101 cgtcagaaga tgaagaatcc cgctgccggt tacgccagag atttcatcag cttgatgaaa
     2161 gagatcggca aagaggccta tgaaaagaag atcggcatca ttaccaatgc cggcggcatg
     2221 aatatcaccg gcgctgtgga cgctctgaag gctgtggccg aggaacagga gatgcacggc
     2281 tacaagatcg gttatgtcac cggtgacgat ctgctggatc agatccctca gatgatcgct
     2341 gacggctgcg agttcaaaaa tatggacgat gtgggcgatt tcaatgagat caaggacaag
     2401 cttgtgaatg ccaatgtcta ctatggacgg gaaccgatca tggagtgtct ggcccagggc
     2461 gcaaatactg tgctgactgg ccgcgccgca gacagcgcaa tgttcctggc acctctggct
     2521 tatgagtttg gctggaaaga taacgatttg gacaatttgg cccggggcat tatggccggt
     2581 catctgctgg aatgtggcgg gcagggctcc ggcggtaact tccagtatga ctggcgcagt
     2641 gtgcccgaca tggatcagtt gggtttcccc atcgctgagc tgacggaaga ggacttccac
     2701 attacgaagg ctcctgattg cggcggcctg atctctgagc agagctgcaa ggaacagttc
     2761 ctctatgagg tccatgaccc tgccaattat atcacccctg atgtcactgt ggacattagc
     2821 catgcgaccc tgacgcagga cggcaataac cgtgtccgcg tgggcaatgt caagggcaaa
     2881 gccagaccgg atcaactgaa gctgtgtctg ggctatcacg ccggctacaa ggtagtcacg
     2941 tatctgtcct tcgcctggcc tgatgcctac gaaaaggccc agtatgctgc ggagatcctg
     3001 atgaagaaga tgaaacgcaa gggaatgcag tatgaggatc tgcgcatcga ctatgtggga
     3061 ctcaatgccc tgcatctgga tgtggcagag atcgatgatg acctcattaa gcgcaccaac
     3121 gaggtggtgc tgcgcattgc catccgcacc aaggaaaaac gggacgccca gctcattatt
     3181 cccgagatca gccccttgca actcaatgga cctccgggag cgtccttctt tggcggtcgc
     3241 gctcatgtga cggatgttat tggactgtgg cccacgctga tcc
//
LOCUS       DBMDQJ010000235         3278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10606_length_3278_cov_1.897387, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000235 DBMDQJ010000000
VERSION     DBMDQJ010000235.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10606_length_3278_cov_1.897387"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            29..1237
                     /locus_tag="ACZO9R_05885"
     CDS             29..1237
                     /locus_tag="ACZO9R_05885"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HIR0912629.1"
                     /translation="MKIWEDERQCYIRAAMEKNSEAENEALKAQLDAIDWSVLEQIER
                     KETVNERGVFAPLEAVETEEIERRRDEFRELGIKAIREGKVGAVLLAGGQGTRLGLDR
                     PKGTLNIGIHRELFLFQQLIRNLMDVTDEAGAYVPLYIMTSNINHDDTQAFFEEHSYF
                     GYPKEYVKFFVQEMEPACDHQGRVYMESRTRVAMSPNGNGGWFGSMVNAGLLSDIRSH
                     GIEWINVFAVDNCLQRIADPLFIGATIAYGCESGAKVVRKAAPDEKVGVLCTEDGKPS
                     IAEYYEMTQEMATARKANGDLLYGFGVILNYVFSEKRLEEIADAHMPIHVVEKKIPYM
                     DESGSLVKPDAPNGYKFETLVLDMVHMMADCVPYEVDRKREFAPIKNLHGVDSLDSAR
                     ELMQGCGIEL"
     gene            1392..2132
                     /locus_tag="ACZO9R_05890"
     CDS             1392..2132
                     /locus_tag="ACZO9R_05890"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR0912630.1"
                     /translation="MEEQGKQQEIEIEEVTAQENVIAVRDLVPMRAAMDDSMERIADM
                     KDQFSQTIDPILRMYNAAMCATTARLEIIEDEFKYRKLRCPIHHIDTRLKSAKSILGK
                     LQKKDLDLTLSAACNNIYDIAGIRVVCSYIKDVYLIRDRLMAQDDVMIMQIKDYIETP
                     KENGYRSLHMVIRVPVYFMNKKQLVPVELQIRTLAMDLWASLEHDIKYKCLYQAETED
                     FSEELKECSRLIYEAEEKMEIMNRTLEA"
     gene            2148..2957
                     /locus_tag="ACZO9R_05895"
     CDS             2148..2957
                     /locus_tag="ACZO9R_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HIR0912631.1"
                     /translation="MKGKQNPVEKEWAAVVKAEERFLRHAMPARTAGWQEKITRYVPQ
                     KLETTLHAAFYKAFELIFDRGTPVIEKTYQREKKEQNYKINAYTAEVRDTRHALRAFG
                     REAGASRNLNLAVSAVEGVGMGFFGLGLPDIPLFLGVLLKSIYEVALSYGYTYDTQEE
                     QIFILKLIETALSHGEQLAQNNMELNLWMREERTFSISRNEQIRRTSDALAGELLYLK
                     FVQGLPVVGMVGGVSDMVYQKRISDYAAVKYKRRFLETKRKTGEEKRHPAG"
     gene            complement(2989..3144)
                     /locus_tag="ACZO9R_05900"
     CDS             complement(2989..3144)
                     /locus_tag="ACZO9R_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912632.1"
                     /translation="MASYVSPEIRDRFDTLSADLKNLILERNVCLNNMYDLIHVLEDI
                     VAEGEAE"
BASE COUNT          874 a          682 c         1021 g          701 t
ORIGIN      
        1 aattgatagt aaaaggagaa aggtttttat gaaaatttgg gaagacgaaa ggcagtgcta
       61 tatccgtgct gccatggaga agaattcaga ggcggagaac gaggcgctga aggcacagct
      121 ggatgcgatc gactggtctg tgttagagca gattgagcgc aaggagaccg tgaacgagcg
      181 gggcgtattc gcaccgcttg aggcggttga gacggaagaa attgagcggc gcagggacga
      241 gttccgggaa ctcggcatca aggcgatccg ggaaggaaaa gtcggagcgg ttcttctggc
      301 ggggggacag ggaacgcgtc tcggactgga cagaccgaag ggaacactca atatcgggat
      361 tcaccgggag ctgtttttgt ttcagcagct catccgtaac ctgatggacg tgacggatga
      421 agccggcgcg tatgttccgc tttatatcat gacgagcaac atcaaccatg acgacacgca
      481 ggcatttttt gaggagcaca gttactttgg ttacccgaag gagtatgtga aatttttcgt
      541 gcaggagatg gaacctgcct gtgatcatca ggggcgcgta tatatggagt cccgtaccag
      601 ggttgccatg tccccgaacg gcaatggtgg ctggtttggt tccatggtga atgcagggct
      661 gctttccgac atccgttccc acgggattga gtggatcaat gtatttgctg tggacaactg
      721 cctgcagaga atcgcagacc cgctgttcat cggtgcaacg attgcctacg gctgtgagag
      781 cggcgcgaag gttgtcagaa aggcagcgcc ggatgagaaa gtgggggtgc tctgtaccga
      841 ggacggcaag ccatcgatcg cggagtatta tgagatgaca caggagatgg cgaccgcgcg
      901 gaaggcaaat ggtgacctgc tctatggatt tggtgtgatc ttaaactacg tcttttccga
      961 gaagcgtctg gaggagatcg cggatgcaca tatgccgatc catgtggtag agaagaagat
     1021 tccgtatatg gacgaatccg gcagcctggt gaagccggat gcaccgaacg gatacaagtt
     1081 tgagacgctt gtcctcgata tggtacacat gatggcggac tgcgttcctt acgaggtcga
     1141 cagaaagcgg gagtttgcgc cgatcaagaa tctgcacggc gtggattcgc tcgacagcgc
     1201 ccgcgagctt atgcagggct gcggcatcga attgtaacat aaaacggacg ggcttcccgg
     1261 cgggaagtcc gtccgttttt ggcgctgcag aagatttaat aaatatttta tggggattta
     1321 acggcgggtt catgaatgtc tgatataata atacctgatg atggaaaaca gacaatggag
     1381 gtttttataa attggaggaa caggggaagc agcaggaaat agaaatcgaa gaggtgaccg
     1441 cacaggagaa tgtgatcgcg gtgcgggatc tggtgccgat gcgtgcggcg atggatgaca
     1501 gcatggaacg gatcgcagat atgaaggacc agttttcaca gacgatcgat ccgatcctgc
     1561 gcatgtacaa tgcggcgatg tgtgcaacga cggcaaggct tgagataata gaggacgagt
     1621 tcaagtaccg gaagctgcgc tgtccgatcc accatattga cacccgcctg aagtcggcga
     1681 agagtattct gggcaagctc cagaagaagg atctggatct gacgctgtcg gctgcctgca
     1741 acaacatcta cgacatcgcg ggaatccgtg ttgtctgctc ttatatcaag gacgtctatc
     1801 tgatccgcga ccgtctgatg gcgcaggatg atgttatgat tatgcagatc aaggattaca
     1861 tagagacgcc gaaggagaac ggttaccgga gtcttcacat ggtcatccgg gtgccggtat
     1921 attttatgaa caaaaagcag ctggtgccgg tggaactgca gatccgcacg ctggcgatgg
     1981 atctgtgggc gagcctggag catgacatca agtataagtg tctgtatcag gcggagacgg
     2041 aggatttctc agaagaactt aaggaatgca gccgcctcat ctacgaggcg gaggaaaaaa
     2101 tggagatcat gaaccgtaca ctggaagcat agcgggaggc gcgggcgatg aaagggaaac
     2161 agaatccggt cgaaaaagaa tgggcagcgg ttgtcaaggc ggaggagcgc tttctgcgcc
     2221 atgccatgcc ggcgcggacg gcgggctggc aggagaagat cacgcgctat gtgccacaga
     2281 agctggagac gactctgcac gccgcatttt acaaagcgtt tgaactgatc tttgacaggg
     2341 gaacgccggt gatagagaag acctatcaga gggagaaaaa ggagcagaat tataagatca
     2401 atgcttacac cgcagaggtg cgtgacacga gacatgccct gcgggcattc gggagagagg
     2461 cgggcgccag ccggaacctg aatctggcgg tatcggcggt ggaaggcgtc ggcatgggat
     2521 ttttcggtct gggtctgccc gatataccgc tgttcctcgg cgttttgctg aaaagcatct
     2581 atgaggtcgc actcagctac ggatacacgt atgacacgca ggaggagcag atcttcatat
     2641 taaagctgat cgagacggca ttgtcgcacg gcgagcagct tgcccagaac aatatggagc
     2701 tgaatctgtg gatgcgtgag gagcgtacgt tttccatttc cagaaacgag cagatccggc
     2761 gcacatcgga tgcactggcg ggagaactgc tgtatctgaa attcgtgcag ggactgccgg
     2821 tagtcggtat ggtcggtgga gtttctgata tggtttatca gaagcgcatc agcgattatg
     2881 cagcagttaa atataaaaga cgttttcttg agacaaagag gaagaccggg gaagaaaaga
     2941 gacatccggc aggataagtt tgcggatggc atgcagaagg ggaagccgtt attcggcttc
     3001 cccttctgcg acaatatcct caagaacatg aatcaggtca tacatgttgt tgagacagac
     3061 attccgttcc agaatcagat ttttcagatc cgcagacagg gtatcaaacc ggtcgcggat
     3121 ctcgggagat acataagatg ccataagatt acctcacttt ttcatacttc agaaaaagta
     3181 tgagcatccg gagagctttt tatgtgtaaa aaatgccgta aaggtggtat gatagtggag
     3241 aaaaagtgga atggaataag agtatgaatg agaaaatt
//
LOCUS       DBMDQJ010000236         3278 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10607_length_3278_cov_1.677683, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000236 DBMDQJ010000000
VERSION     DBMDQJ010000236.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10607_length_3278_cov_1.677683"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            59..2860
                     /locus_tag="ACZO9R_05905"
     CDS             59..2860
                     /locus_tag="ACZO9R_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR0912633.1"
                     /translation="MKKKKTRNPLIRRIPRELAGDWRKYLLVSLFLILTIGFVSGMYV
                     ANESMMKAADEGVTKYKLEDGHFELNEEADQTLLAAIATGEKADVKQYYTDKAKRELD
                     EKFDDEFEDKFKDEFDSQFKEEFDRKFNAEFQTQFDQTFAAQVKQTLLAQGMDETMAA
                     AMLDTAVAQAKQAGSYQQTYDAAYESAYQKAYDTAYDEAYGKAHDEAYEKAYDEAWDK
                     ILDEIDEKYQKAEDKYELNDPDFAAVPVNLFENFYRNEEEDHDGDGVPDGNVRVFKKT
                     DEVNLACLLEGSFPASSDEIVVDRMHADNVGIAVGDTITVQGEAYRVVGLIAYVNYAT
                     LHEKSTDLMFDALKFNVAMVTEDGFARLHEKVHYDYAWNYVTEPADEAAEKNLSDDFM
                     KALLTQVVVADAEIEDYVPEYANPAIHFATDDMGSDEAMGGVLLDILIVIIAFIFAIT
                     ISNTIARESATIGTLRASGYTRGELVAHYLSMPVIVTLVAALIGNILGYTVFKNIVVS
                     MYYNSYSLPTYETIWNPDAFIKTTLIPLILMFVVNLVVIARMMRHTPLQFLRHDLKKT
                     KRKKAMRLPRFQFLSRFRLRVMFQNMANYAILFVGIFFCMVMLAMAVGMPDTLGYYKE
                     NAKDLMFAKYQYVLASWEDEDGDVITTKEPGAEKFSMTSLLRKSDTLDEEVSVYGISD
                     DSRYVKIDDLTHLKENEAYISASYADKYDVSVGDDVTLDEKYENQQYTFRVAGIFDKS
                     QSIAVFLPIENYREVFEQDADAFDGYFSDTEITDIAEENIATVITEQDITKMCDQLDH
                     SMGSYMSYFQILCILLSAVLIYLLTKIIIEKNETAISMTKILGYENREIASIYLLSTT
                     IVVIVADAICVVLGALVMNAAWKVIMYSYSGWFSFVILPAGYMKMFAFVLIGYLIVMV
                     FDFMRIRKIPMDQALKNVE"
     gene            2966..>3278
                     /locus_tag="ACZO9R_05910"
     CDS             2966..>3278
                     /locus_tag="ACZO9R_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HIR0912634.1"
                     /translation="MDKIEIYIDELLEKSTPDRPIWNIEKILQGLKSTWNYIDGCMIK
                     AILEMYAITKDEKYFRFADNFIDCKVREDGSIEGYSVEELNIDNVNAGKTLFELYDLT
                     LQ"
BASE COUNT          897 a          642 c          927 g          812 t
ORIGIN      
        1 agatcaacac ggataaagtt tcagcggcag atctggaatg gtaaggagtg tgaccggcat
       61 gaagaagaaa aagacaagaa atccgctgat cagacgcata ccgagggaac tggcgggaga
      121 ctggagaaaa tatctgctcg tgagcctgtt tctcattttg acaatcggct ttgtatcggg
      181 aatgtacgtt gccaacgaga gcatgatgaa ggctgcagat gagggagtca cgaaatataa
      241 gctggaggac ggacattttg aactgaatga ggaggcggat cagacactgc tcgctgcgat
      301 tgccacggga gagaaagcgg atgtaaagca gtattacacg gacaaggcaa agcgtgagct
      361 ggatgagaag tttgacgatg aatttgagga taagttcaag gacgaattcg acagccagtt
      421 caaggaggaa tttgaccgca aattcaatgc ggaatttcag acacagttcg accagacatt
      481 tgcggctcag gtaaaacaga cgctgctggc acaggggatg gatgagacta tggctgcggc
      541 gatgttagat acggcggtgg cgcaggcaaa gcaggcggga agttatcagc agacctacga
      601 tgccgcttac gagagcgcgt atcagaaagc ctacgatacc gcctatgacg aggcgtatgg
      661 gaaggcacat gacgaggcgt acgagaaggc ttacgacgaa gcttgggaca agattctgga
      721 tgaaattgac gaaaaatatc aaaaggcgga agacaaatac gaattgaacg atcctgattt
      781 tgcggcggtt ccggtgaatt tatttgaaaa tttttaccgg aatgaagagg aggatcacga
      841 cggcgacggc gtcccggacg ggaatgtccg cgttttcaaa aagacggacg aggtcaatct
      901 ggcgtgtctg ctggaaggaa gcttcccggc atcttccgat gagattgtgg tggaccgcat
      961 gcacgcggac aatgtcggca ttgcggtggg agataccata acggttcaag gagaggcgta
     1021 tcgtgtcgtc ggactgatcg cctatgtcaa ttatgccaca ctgcatgaaa aaagtacgga
     1081 tctgatgttt gatgcgctga aattcaatgt ggcgatggtg acggaagacg gatttgcgcg
     1141 cctgcacgag aaggtgcact atgattatgc atggaactat gtgacagaac cggcggatga
     1201 ggcggcagaa aaaaatctgt cggatgattt tatgaaagcc ctgctgacgc aagtggttgt
     1261 cgcggatgcg gagattgagg attatgttcc ggaatatgcg aaccctgcca ttcattttgc
     1321 aacggatgac atgggctctg atgaggcgat gggtggtgtg ctgctcgata ttctgattgt
     1381 gattattgct tttatttttg ccattacaat cagcaacacg attgccaggg agtcggctac
     1441 catcggaaca ctccgcgctt cgggttacac gcgcggggaa ctggtggcac attatctgtc
     1501 gatgccggtt attgtgacgc tcgtcgcggc gctgatcgga aacattctcg gatatacggt
     1561 gttcaaaaat attgtggttt cgatgtacta taacagctac agccttccga cttacgagac
     1621 gatatggaat ccggatgcgt ttatcaagac gacactgatt ccgctgattc tgatgtttgt
     1681 tgtaaacctt gtggtgattg cgcggatgat gcgccatacg ccgttacagt ttttgcggca
     1741 tgatttaaag aagacaaagc gcaaaaaagc gatgcgcctg ccgcggtttc agtttttgag
     1801 ccgtttccgc ctgcgcgtca tgtttcagaa tatggcgaat tatgcgatcc tttttgtcgg
     1861 aatattcttc tgtatggtca tgctggcgat ggcggtcggc atgccggata cgctcggcta
     1921 ttacaaggaa aatgccaagg atctgatgtt cgcgaagtat cagtatgtgc ttgcgtcctg
     1981 ggaggatgag gacggcgatg tcatcacaac aaaggagccg ggtgcggaaa aattcagcat
     2041 gacatctctt ttacggaaga gtgatacact cgatgaggaa gtatctgtct acggcatctc
     2101 cgatgacagc cggtatgtaa aaatcgatga tcttactcat ttaaaagaga acgaggcata
     2161 tatttccgcc tcctatgcag acaaatacga tgtctctgtg ggcgacgatg tcacactgga
     2221 tgaaaagtac gagaatcagc agtatacctt ccgggtggcg ggcatttttg acaagtccca
     2281 gagcattgcc gtatttctgc cgatcgaaaa ttaccgtgag gtatttgagc aggatgcgga
     2341 tgcgtttgat ggctatttct cagacacgga gattacggac attgcggagg aaaatatcgc
     2401 gacggtaatt acggagcagg acatcacgaa aatgtgcgat cagctggatc actcgatggg
     2461 atcgtatatg agttatttcc agatcttatg catcctgctg tctgcggtgt taatctatct
     2521 tctcacaaag attatcattg agaagaatga gacggcaatt tccatgacaa agatcctggg
     2581 ctatgagaac cgggagattg ctagcattta tctgctgtcg accacgattg ttgtgatcgt
     2641 ggcggatgca atctgcgtgg tgcttggcgc gctcgtgatg aatgccgcct ggaaagtgat
     2701 catgtacagt tacagcggat ggttttcttt tgtcattctt ccggcgggct atatgaaaat
     2761 gttcgcgttt gttctgatcg gttatctgat cgtgatggtg tttgacttta tgcggatcag
     2821 gaaaattccg atggatcagg cattaaaaaa tgtcgagtga catccgcccg gggaaaagag
     2881 agggatatct attgcatagt ttccgaaatt cttctataat caaggggatg ttttttgaaa
     2941 aatccccgag ataaaggagc agaacatgga caaaatagaa atctatattg acgaattact
     3001 ggagaagagt accccggatc gcccgatctg gaatattgag aagattctgc agggcttaaa
     3061 gtccacctgg aattacatag acggatgcat gatcaaggcg attcttgaaa tgtacgcgat
     3121 cacaaaagac gagaaatatt ttcgctttgc cgacaacttt atcgactgca aggtcagaga
     3181 ggacggatcg attgagggct attcggtaga agaattaaat attgacaatg tcaatgcggg
     3241 aaaaaccctg tttgagctct acgatctgac cttgcagt
//
LOCUS       DBMDQJ010000237         3268 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10654_length_3268_cov_1.485949, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000237 DBMDQJ010000000
VERSION     DBMDQJ010000237.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10654_length_3268_cov_1.485949"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            174..1073
                     /locus_tag="ACZO9R_05915"
     CDS             174..1073
                     /locus_tag="ACZO9R_05915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912635.1"
                     /translation="MRTICYVILNYKTYEEAAACAESILSTQTWKNMHIVIVDNGSGN
                     GSEEWLAEHFIDEKRVRVIASGENLGFARGNNLGIRYAREHYDPDLIVAANSDILFEQ
                     PDFCERLFEIYDRKPYAILGADVVDATRTQHFNPVAEKRSYTLNYMRKQILSSWIRAM
                     SYRMSKLFLGKKNGGDLSRGTGATREVDGEAVAADSRVGEEREGVLLHGCCLVFSRDF
                     FKVYDGFWEGTFLYAEEEILYYLAAKSGLRMLYSPEVTCIHKEAVTTRKLYSDVCDAK
                     IFYYTQITKSYRAFLGLMKKYEK"
     gene            1063..1830
                     /locus_tag="ACZO9R_05920"
     CDS             1063..1830
                     /locus_tag="ACZO9R_05920"
                     /inference="COORDINATES: protein motif:HMM:NF025089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912636.1"
                     /translation="MKNKITIVCCYNDRKQYGRLQASLAKQNTGYELIGMDNCSRQYA
                     SCSLALNAATARVETEYVIYAHQDVELPGEDTLARFAGYLAQTGKEDLLGVAGIVPGT
                     IPGRKKRFGEAEGIVLSGVQHGAGELAAAGEQAFSGMRACDSLDECFFGGHTAHFKKN
                     PFDEKLCDNWHLYGVERCLHTRWHGGNVYVCDVALIHHSTGHINHAYNENFRRLAKRY
                     AGMQCIRTVCGSSKTGVWHRNVFYWKRELLIRLGRLG"
     gene            1854..>3268
                     /locus_tag="ACZO9R_05925"
     CDS             1854..>3268
                     /locus_tag="ACZO9R_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015013.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HIR0912637.1"
                     /translation="MAEKKSLVKNSIFNMIYKGFTALFPLITTSYISRVLLPAGVGRV
                     GYANTIVAYFVLIASLGIPTYGVKAIAQSGETKEGRSRTFWELFFINLAATLMCIVAY
                     YVFVDNFPHFADRKLLFQIMGLMLVLNIFNIDWFYQGMEEYSYIATRSIIVKILSFVA
                     MLLFVKKAKDYVIYAFILCVATAGNNLLNAWNLRKYIGKPDVGLHPGRHLKPVLILLA
                     STIATEIYTMLDTIMLEYYHGETCVGYYSNPVKIVRMTYTVVIALVAVFYPRISYHYK
                     QKEYDRCNELLSRGFKIMLLLALPCTVGIGITAEYIVPFLFGEAFLPSIGTLRILSVL
                     VLVFSVAYFLGHIVLIATGQEQKILHATIAGAVINAAVNFLLIPSMQQSGAAIASVLS
                     EIAVTTVLVWHARCSYHLAIGRNYVISLGAALAAMTAVVCWLKHLSWAVPVMLFVLIA
                     AAAAVYFGCLLLFRNDTVMELL"
BASE COUNT          760 a          749 c          989 g          770 t
ORIGIN      
        1 ttaagctcaa ggagagcaga aagggactgc cgtttcggga ctggttccat ttcctgcaca
       61 agtattttgg actcggaacg gcaatcgtgc gttccatcac cccgtatgtc cgcattatcc
      121 ttggcacgaa gaccagatac tcgggcgcat aagaattcag ggagacagtg ccgatgagaa
      181 cgatttgcta tgtcatctta aattacaaaa catacgagga agccgccgca tgtgcagagt
      241 cgatcctttc gacgcagacc tggaaaaaca tgcatattgt catcgtggac aacggttccg
      301 gaaacgggag cgaggagtgg cttgcggaac attttatcga tgaaaaacgg gtgcgcgtga
      361 tcgcgtcggg agagaatctc ggatttgcca gagggaacaa tctcggcatc cgctatgcga
      421 gagagcacta tgatccggat ctgatcgtcg ctgcaaacag tgacatcctg tttgaacagc
      481 cggatttttg tgagcgcctg tttgagatct acgacagaaa gccgtatgcg atactgggcg
      541 cggatgttgt ggacgcgacc cgcacgcagc actttaaccc ggtggcggag aagcgcagct
      601 ataccttgaa ctatatgcga aagcagatct tatcatcctg gatccgggcg atgagctacc
      661 ggatgtccaa gctgttcttg gggaagaaga acggaggcga tctgtcgcgt ggcaccggag
      721 cgacccgcga ggtggacggc gaggcggtag cggcggacag cagagtcggc gaggagcgcg
      781 agggagtgct gctccacgga tgctgccttg tgttttcaag agatttcttc aaagtgtatg
      841 atggcttctg ggaaggcacc tttttgtatg cggaggagga gattctctac tatctggcgg
      901 caaagagcgg actgcggatg ctgtattcgc cggaggtcac ctgcattcat aaagaggctg
      961 tgacgacaag gaagctgtac agcgatgtat gcgatgccaa gattttttac tatacacaga
     1021 tcaccaaatc gtaccgggcg tttctcggtc tgatgaaaaa atatgaaaaa taagataacg
     1081 atcgtctgct gctacaacga caggaaacaa tacgggaggc tgcaggcgtc ccttgcaaaa
     1141 cagaataccg ggtatgagct gatcggcatg gacaactgca gcagacagta tgcatcgtgc
     1201 agccttgcac tgaatgccgc gacggcgcgc gtggagacgg agtatgtgat ctacgcccac
     1261 caggacgtcg aacttccagg ggaggatacg ctcgcgcggt ttgccggata cctggcgcag
     1321 accgggaagg aggatctgct gggcgttgcc gggattgtgc cggggacgat tccgggaagg
     1381 aagaagcggt ttggcgaggc cgagggaatc gttttatccg gagtacagca cggcgcagga
     1441 gagcttgccg cagccggcga gcaggcattt tcgggaatga gggcgtgcga ttcgctggat
     1501 gaatgttttt tcggcgggca cacggcacat ttcaaaaaga atccgttcga tgaaaagctg
     1561 tgtgataact ggcatctgta cggcgtggag cgctgtctgc ataccagatg gcacggcggg
     1621 aacgtctatg tctgtgacgt ggcgctgatc catcactcga cggggcatat caatcacgca
     1681 tacaacgaga atttccgccg gctggcaaag cgctacgccg gaatgcagtg tatcaggacc
     1741 gtgtgcggca gcagtaaaac cggggtctgg caccggaatg tgttttattg gaagagggaa
     1801 cttctgatcc gcctcgggcg gctgggatag acagaatcat agaggaagaa cgtatggcag
     1861 agaagaaatc actggtaaaa aactctatat ttaatatgat ctataagggc tttacggccc
     1921 tttttccgct tattaccact tcctatatct cgagagttct tctgccggca ggcgtgggcc
     1981 gcgtcgggta tgccaacacg attgtggcat attttgtgct gattgcgtcg ctcgggattc
     2041 cgacctatgg cgtaaaggcg atcgcgcaga gcggtgagac aaaggagggg cgaagccgca
     2101 ccttctggga actgtttttc atcaatcttg ccgcgacact gatgtgcatt gtggcgtact
     2161 atgtgtttgt ggacaatttt ccgcattttg cggaccggaa gctgttgttt cagatcatgg
     2221 gtctgatgct ggttttaaat atttttaaca ttgactggtt ctatcagggt atggaggagt
     2281 acagttatat tgcgaccaga agcatcatcg tgaaaattct ttcgtttgtg gcgatgcttc
     2341 tgtttgtaaa gaaggcaaaa gattatgtga tctacgcttt tatcctctgc gtggcgacgg
     2401 cgggaaacaa cctgctaaac gcgtggaatc tgcggaaata tatcggaaaa ccggacgtgg
     2461 gtctgcatcc gggcagacat ttaaagccgg tgctgattct gctggcgtcc acgatcgcca
     2521 cggagatcta cacgatgtta gataccatca tgctggaata ttaccacggg gagacgtgcg
     2581 tgggatatta ttccaatccg gtgaagatcg tgcgcatgac ctacacggtg gtgatcgcgc
     2641 ttgtggcggt tttctatccg cgcatcagct atcactacaa gcagaaagaa tatgacagat
     2701 gcaacgaact gctcagccgg ggttttaaga ttatgcttct tctggcactg ccgtgtacgg
     2761 ttggaattgg tattacggca gagtatatcg tgccgtttct gttcggagag gcatttctgc
     2821 cgtcgatcgg aacactgcgg atcttgagcg tgctggtgct ggtgttctcg gtggcgtatt
     2881 tcctgggaca catcgtgctg atcgcaaccg ggcaggagca aaaaattctt cacgcaacga
     2941 ttgcgggggc ggtaatcaat gcagcagtca acttcctgct gattccgtcc atgcagcaga
     3001 gcggtgccgc gattgcatcc gttctgtcgg agatcgccgt gaccacagtg ctggtgtggc
     3061 atgcaagatg cagctatcac cttgcgatcg gcaggaacta tgtcatcagt ctgggcgcgg
     3121 cgctggcagc gatgacggcg gtggtatgct ggctcaaaca tctgtcatgg gcggtgcctg
     3181 tcatgctgtt cgttctgatt gcggcggcgg cagcggtcta tttcggctgt ctgctccttt
     3241 tccggaacga caccgtgatg gagctgct
//
LOCUS       DBMDQJ010000238         3265 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10666_length_3265_cov_1.413401, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000238 DBMDQJ010000000
VERSION     DBMDQJ010000238.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3265)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3265)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3265
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10666_length_3265_cov_1.413401"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2018)
                     /locus_tag="ACZO9R_05930"
     CDS             complement(<1..2018)
                     /locus_tag="ACZO9R_05930"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR0912638.1"
                     /translation="MQKKLIVMFTIVAGMLIGLIGRLMYIEYTSGEKYEKIVLSQQEY
                     DSKVIPYQRGDIVDAKGTVMATSIAVYNVILDCSVLTSDEDYVAPTIQALVQCFPDLS
                     SDELYGYVRNDPQNRYIVLKKKISYDEMQPFVELQDATDEKGRKVNPDIKGVWFEKEY
                     QREYPYGSLASAVIGFTASGNVGVNGLENYYNSTLNGINGREFGYLNTDNNFEKTIKQ
                     ATNGNNIVTTIDANIQSVVEGKIREFNEAYTGAYREGDAGASHIGVLIMDPNNGDVLA
                     MANYPNFDLSNPRDLSAYYTEEELAAMDDDAKMDALNQLWQNFCVSYTYEPGSTAKPF
                     TVSAGLETGTVTTADTYYCDGYETISGHDIHCVKRDGHGMETLEQTLMNSCNDALMQM
                     SYKIGRDNFENYQQIFGFGQKTNIDLPGETRTDSLIYTGDNMTVVDLATNAFGQNFNT
                     TMIQLATGFCSIVNGGKFYQPHVVKRITDENGNVVQEIQPTLLRETISESTSETLRQY
                     MYSTVTSGTGATAKVDGYSMGGKTGTAQKAGRDGVNYLVSFIGFAPVDDPQLVIYCVV
                     DEPNAVEQYHSSFAQNIVREILEEVLPYMNIYRDEQTTGIHAGWDITGTDTGATAMTD
                     VVNEETPVEEGLDVPDTTDTLPGNEEEAEGTDTPQDSGDTPQDNGDEP"
     gene            complement(2081..2623)
                     /locus_tag="ACZO9R_05935"
     CDS             complement(2081..2623)
                     /locus_tag="ACZO9R_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912639.1"
                     /translation="MRNEAAERKYYIDGNTVRRMEAAPAVTRPDRRIRESRPEHVTRE
                     RRERERREREREIELRNRKRAARRNQERALRMSKSYVAFLTMAVMVFGIFTGAYIHIQ
                     SEMTARMNQISSLESQIADLKADNDEAYKRINTSVDLDSIRNTAMTEYGMSYAKESQI
                     VYYTVNDDDYMNQYSEIPRE"
     gene            complement(2648..>3265)
                     /gene="rsmH"
                     /locus_tag="ACZO9R_05940"
     CDS             complement(2648..>3265)
                     /gene="rsmH"
                     /locus_tag="ACZO9R_05940"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR0912640.1"
                     /translation="GRRMMVPVEGTEQELPCELVLIAAGFLGTQKYIADAFGVELNER
                     TNVKTAADKFATNIAKHIVNERQKKPITTAGELSEIIRESIPMKVQKTGGNPAKRTFQ
                     AIRIELNHELEVLQDNLDTMIDLLNPGGRICIITFHSLEDRIVKTNFKRNENPCTCPS
                     DFPVCVCGKKSKGRVITRKPVLPGERELAENSRSKSAKLRVFERA"
BASE COUNT          659 a          967 c          676 g          963 t
ORIGIN      
        1 tgcggttcat ccccgttatc ctgcggcgtg tctccgctat cctgcggcgt gtctgtgcct
       61 tccgcctcct cttcatttcc cggcagggta tccgttgtgt ccggcacatc cagtccttct
      121 tccaccggag tctcctcgtt tacaacgtcc gtcatcgctg tcgctccggt atctgttccc
      181 gtaatgtccc accctgcatg aattccggtg gtctgctcat cgcggtagat gttcatgtaa
      241 ggaagcactt cctctaagat ctcacgcaca atattctgtg caaaagagct gtggtactgc
      301 tccaccgcat tcggctcgtc gaccacgcag taaatcacaa gctgcggatc gtcgaccggt
      361 gcaaatccaa taaacgatac cagatagttg acgccgtcac gtcccgcttt ctgtgccgtt
      421 cccgtcttgc cgcccattga gtagccgtcc actttcgctg tcgctccggt acctgaggta
      481 acagtggaat acatatactg ccgcagcgtc tcggacgtac tctccgagat ggtttcccga
      541 agaagtgtcg gctggatctc ctgcacaacg ttgccgttct catccgtaat ccgttttacc
      601 acatgcggct gatagaactt tccgccgttt acaatcgagc agaagccggt ggcaagctgg
      661 atcatcgtcg tgttgaagtt ctgaccgaat gcgtttgtcg caagatccac caccgtcatg
      721 ttgtcgccgg tatagatcag agaatcggtt ctcgtctctc ccggcagatc aatgttggtc
      781 ttctgaccaa atccgaaaat ctgctgataa ttttcaaagt tatctcttcc tattttatag
      841 gacatctgca tcagcgcatc gttgcaggaa ttcatgagtg tctgctccaa tgtctccatt
      901 ccgtgtccgt ctctcttgac acagtgaatg tcatgaccgg agatcgtctc atacccgtcg
      961 cagtaatagg tgtctgccgt ggtcaccgtc ccggtctcaa gtcctgccga cacggtaaaa
     1021 ggcttcgccg tcgatcccgg ttcataggtg tacgacacac agaagttctg ccacagctgg
     1081 tttagtgcat ccatctttgc gtcatcatcc atggcagcga gttcttcctc ggtgtaatac
     1141 gcggagagat ctctcgggtt gctcagatca aaattcggat aattcgccat ggcgagcaca
     1201 tccccgttat tcggatccat aatcaggact ccgatatggg aagctcccgc atccccttcg
     1261 cggtaggcgc ctgtgtacgc ctcgttgaat tcccggatct tgccctccac cacggactgg
     1321 atattcgcat caatcgttgt cacgatattg ttgccgtttg tcgcctgctt gatcgttttc
     1381 tcaaaattat tatccgtatt cagatagccg aactcacgcc cgttgatgcc gtttaacgtg
     1441 ctgttgtaat aattctccag accgttcact ccgacatttc cggatgccgt aaatccgatc
     1501 accgcgctgg cgagcgatcc gtacggatac tctctctgat attccttctc aaaccatacg
     1561 cccttgatgt cggggttcac ctttctgccc ttttcatccg tcgcatcctg cagttccacg
     1621 aacggctgca tctcgtcgta cgatattttc ttcttcagca caatataacg gttctgcgga
     1681 tcattccgga catatccgta aagctcatcg ctgctcaagt ccggaaaaca ctgcacaagc
     1741 gcctgaatcg tcggcgcaac gtaatcctca tcgctcgtta aaacagaaca atccagtatc
     1801 acattgtaca ctgcaatact ggttgccatc actgtcccct ttgcatcgac aatatcgcct
     1861 ctctggtaag gaatcacttt gctgtcgtat tcctgctggg acaacacaat tttctcatat
     1921 ttttcaccgc tggtatattc aatatacatc aggcgcccga tcagaccgat cagcatgcct
     1981 gccacaatgg taaacatgac aatcagcttt ttttgcatgc gcctgggaaa tttcaacagc
     2041 ggtcttcttt ttttctttgc catgcgcctc tcctgtcatc ctactcgcgc ggaatctcac
     2101 tgtactggtt catgtaatcg tcatcgttca ctgtatagta cacaatctgc gattccttcg
     2161 cataggacat tccatattct gtcatcgccg tattccggat gctgtcaaga tccacgctcg
     2221 tattgatccg cttgtatgcc tcgtcgttgt cggctttcag atctgcgatc tggctctcaa
     2281 ggcttgatat ctgattcatg cgcgccgtca tctccgactg gatgtggatg tatgcaccgg
     2341 taaagatgcc gaacaccatc acagccatcg tgagaaatgc cacatagctc ttgctcatgc
     2401 gcagagcacg ctcctgattt cttctggctg cacgctttct gttccttaat tcaatctcgc
     2461 gttcccgctc ccgtctctcg cgctcccttc tctctcttgt gacatgctcc gggcggctct
     2521 ctctgatcct gcgatccggt ctcgtcactg caggcgctgc ttccatcctg cggacagtat
     2581 tcccgtcaat ataatatttt ctctctgccg cttcatttct cataactgcc acttccctca
     2641 ctacactcta agctctttca aatacgcgga gctttgcact cttcgatctc gagttctctg
     2701 caagctctct ctcccccggc aatactggct tccttgtaat cactcttccc ttggactttt
     2761 tcccacatac acataccggg aaatcactcg ggcaggtaca tggattctcg ttgcgcttaa
     2821 aattcgtctt gacgatccgg tcctctaatg agtgaaaggt aataatacag atccttcctc
     2881 ccggattaag cagatcgatc atcgtatcca gattgtcctg caacacttcc agttcgtgat
     2941 tcaattcaat tctgattgcc tgaaacgtac gctttgccgg atttcctccg gtcttctgca
     3001 ctttcatcgg gatggactcc cgaataattt ccgacagttc tcccgccgtc gtaatcggct
     3061 tcttctgtct ctcgttcact atatgttttg caatatttgt tgcaaattta tctgccgcag
     3121 tctttacatt ggttctttcg tttaattcca caccaaatgc gtctgcgata tacttctgtg
     3181 tgcctaagaa acctgccgca atcagtacga gttcgcaagg cagttcctgt tctgttccct
     3241 ctaccggaac catcattctt ctgcc
//
LOCUS       DBMDQJ010000239         3264 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10672_length_3264_cov_1.434714, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000239 DBMDQJ010000000
VERSION     DBMDQJ010000239.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10672_length_3264_cov_1.434714"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            100..2088
                     /locus_tag="ACZO9R_05945"
     CDS             100..2088
                     /locus_tag="ACZO9R_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR0912641.1"
                     /translation="MIIGTVLCAMVLALGNRMPVMAEEVVSEELKAADTLNLTVSYPS
                     DIKCGEEVTFKLLATGGSGTYKYRIASLTDAQQNFVYDISYGSNSVYGDSDEFKFTFY
                     ASGTYYIRFGVMDMGSTPYQTKTTGLLEYPIVINDPNYPSVEELVANVAGECEKSCST
                     DFDKAVWLHDWILDHADYDYTYSYCAAEGVLARGKGTCESYHRAYVMLLNKVGIPTGR
                     IAGNGHVWTAAQLDGKWYQIDSTWDDMGASYKGTFYEHLYFGLNDDIMKLVHSDHTQP
                     VEGYECNSLEENYFIKTGEIHQWSDPFAEKIRQKIAAGETSFTLAVNSDLPESVVNVI
                     YRLVAYELSSESWTNATLSASYDNQKLTCSVTVKTPENNGGDNGNSGSNDSNNGGNTG
                     GNSGSNDSNNGGNTGTDNGNSGNTGTTLTGKQRFASLLYENALGRSAAQSELDYWAQE
                     LTNGRTGAEVAYGFLFSEEFQNHNYNNADYVEHLYLSLMGRASDSDGKAGWVKTLENG
                     TSRLYVFRQFINSEEFQQLCNMYEIQKGDVSLTEERDQNYNVTSFVARNYTQFLSRNY
                     DTDGLNHWCEAINHHTQSMQEIAYGFVFSTECSNKNLSNTEYVKMLYRGCFDREGDDA
                     GISDWTNALNSGMMDRTQVFWGFANSQEFANMVESYHL"
     gene            2107..3048
                     /locus_tag="ACZO9R_05950"
     CDS             2107..3048
                     /locus_tag="ACZO9R_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR0912642.1"
                     /translation="MSKALIVGAAGFVGSYLAREVKDHHGMEVHATKLAHEQLEIPGV
                     AIHDLNILNKDEIVALLYEVRPDYIFHLAAQSSVSVAWKNPGLTIDINVKGSVNLMDA
                     VRELFYKPRVLLIGSGEEYGHIREGETPIKEDNYLRPGNIYAATKVCQNMIASIYTQA
                     YDMQLMMVRAFNHIGPCQSPIFVISDFCKQVAEIEAGKKEPVMYVGNLAAKRDFTDVR
                     DVVRAYALLVQSGTAGETYNVGSGHAHEIREILDLIISLSTKEIRVEIDPNKIRPVDV
                     PIIEADITKLNAATGWKPQIPLEQTIQEILDYWRERV"
     gene            3050..>3264
                     /locus_tag="ACZO9R_05955"
     CDS             3050..>3264
                     /locus_tag="ACZO9R_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912643.1"
                     /translation="MMEEIERMEKMFSSEGVTKSDRMLHTPGMFAKNHLLYVQEVGRL
                     ESLVPHVCRRENLDSYLIFEVVAGKGS"
BASE COUNT          944 a          614 c          946 g          760 t
ORIGIN      
        1 actgccagaa ttttcttgga gactggtaaa caataaaaaa agaaaggggg gaattccggg
       61 cggaattccc ttttgaggta caaaatgaga aaaataagaa tgataattgg cacagtattg
      121 tgcgctatgg tgctggcact cggaaaccgt atgccggtaa tggcagagga agtggtatct
      181 gaggaattaa aagcggcaga cacgctgaat ttaacggtat cttatccatc agacatcaag
      241 tgcggggaag aggtgacatt taagcttctg gcgaccggcg ggagcggtac ttacaaatac
      301 cggattgctt cgctgacgga tgcgcagcag aattttgtct atgatatcag ctatgggtca
      361 aacagtgtct acggagatag cgatgagttc aaattcacct tttatgcgtc aggtacgtat
      421 tatatccggt ttggtgtcat ggatatggga tcgacaccat accagaccaa gacgacaggg
      481 ctgctggagt accccatcgt aattaacgat ccgaattatc catcggtgga agaactcgtg
      541 gcaaatgttg cgggcgaatg tgagaagagc tgctcgaccg attttgataa ggcggtctgg
      601 ctgcatgact ggattttaga tcacgcggat tacgactata cgtacagcta ttgtgcggca
      661 gagggcgttc tggcgcgcgg aaagggaacc tgtgagtcat atcacagagc ctatgtgatg
      721 cttttaaata aggtcggtat tccaacggga cggatcgccg gaaatggaca tgtgtggaca
      781 gcggcgcagc tggatggaaa atggtatcag attgacagca cctgggatga tatgggagcg
      841 tcctacaaag gaacgtttta tgagcatctg tattttggat tgaatgacga tatcatgaag
      901 ctggtacaca gtgaccatac acagccggta gaaggatatg agtgcaactc tctggaagag
      961 aattatttta ttaagaccgg ggagatacat cagtggtcgg atccatttgc tgagaagatc
     1021 cgtcagaaaa ttgcagcagg ggagaccagc tttacacttg cggtgaatag tgatctgccg
     1081 gagagtgttg tcaatgtgat ctaccgtctc gtggcgtatg aactttccag tgagagctgg
     1141 acgaatgcaa cgctgagtgc atcttatgac aatcagaaac tgacctgcag cgtaaccgta
     1201 aaaacaccgg aaaacaacgg tggagacaat ggaaacagcg gatcgaatga cagtaacaat
     1261 ggcggaaata caggtggaaa cagcggatcg aatgacagta acaatggcgg aaacacaggt
     1321 acggacaacg gaaacagcgg caacacagga acgacgctga ccggcaagca gcggtttgca
     1381 tcacttttat atgaaaatgc actcggcaga tcggcagcac agagcgaact ggattattgg
     1441 gcgcaggagc tgacaaacgg aagaacgggt gcggaagtgg catacggctt tttattcagc
     1501 gaggagtttc agaatcataa ttataacaat gccgattacg tggagcatct gtatctgagc
     1561 ctcatgggac gtgcatcgga ttcggacgga aaagcgggct gggtcaagac actggaaaac
     1621 ggaacctcca gactttatgt gttccgccag tttatcaatt cagaggaatt ccagcagctg
     1681 tgcaatatgt atgagattca gaagggcgat gtctcattga cggaagaacg cgaccagaac
     1741 tataatgtta caagttttgt cgccagaaat tatacacagt ttttaagcag gaattatgat
     1801 acggacggtt tgaaccactg gtgtgaagcg atcaaccatc acacgcagtc catgcaggag
     1861 attgcttatg gatttgtctt ttccacagag tgcagtaata agaatttgtc gaataccgaa
     1921 tatgtaaaaa tgctgtaccg cggatgcttt gacagagagg gagatgatgc gggaataagc
     1981 gattggacaa atgcactgaa ctctggtatg atggacagga cacaggtgtt ctgggggttt
     2041 gcaaattcac aggaatttgc aaatatggta gaaagttatc atctataagt aaaagggaat
     2101 gacaatatga gcaaagcatt gattgtaggt gcggcaggtt ttgttggaag ttatcttgca
     2161 cgtgaggtaa aagatcatca tggaatggag gttcatgcga caaagctggc gcatgaacag
     2221 ttggagatac cgggagtggc gattcatgac ttaaatattt tgaataagga tgagatcgtt
     2281 gcattgctgt acgaggtgcg cccggattat atttttcatc tggcggcaca gagttcggtc
     2341 agtgtagcat ggaagaatcc ggggcttacg attgacatca atgtcaaggg aagcgtgaat
     2401 ctgatggatg cggtgcggga gcttttttac aaaccgcgcg tacttctgat cggatccggt
     2461 gaagagtacg ggcatatccg ggaaggggag acgccgatca aagaggacaa ctaccttcga
     2521 ccgggcaata tctatgcggc aacgaaggtc tgccagaata tgatcgccag catctatacg
     2581 caggcgtatg atatgcagct gatgatggtc cgggcattca atcacatcgg accatgtcag
     2641 tcaccgatct ttgtcatctc ggatttctgc aagcaggtag cggagatcga ggcgggaaag
     2701 aaagaaccgg tgatgtatgt cggaaatctg gcggcaaagc gggattttac ggatgtacga
     2761 gatgttgtgc gtgcatatgc gcttctggta cagagcggaa ctgcaggtga gacttacaat
     2821 gtcggcagcg gacacgctca cgagatccgg gagattctgg atctgattat ctcgctttca
     2881 acaaaggaga tccgggtgga gattgacccg aacaagatcc ggccggtcga cgttccgatc
     2941 attgaggcgg atattaccaa attaaacgcg gcgaccggat ggaaaccgca gattccgctg
     3001 gagcagacga ttcaggaaat tctggattac tggcgggaga gagtatagcg tgatggagga
     3061 gattgagcgg atggaaaaga tgttttcttc ggaaggggtt acaaagtcag accgtatgct
     3121 tcacacacct ggaatgtttg ccaaaaatca tttgctctat gtgcaggagg taggaagact
     3181 ggaaagcctc gtaccgcatg tgtgcaggag ggagaatctg gattcttatc tgatttttga
     3241 agtggtagcg ggaaagggaa gcat
//
LOCUS       DBMDQJ010000240         3256 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10691_length_3256_cov_1.303645, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000240 DBMDQJ010000000
VERSION     DBMDQJ010000240.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10691_length_3256_cov_1.303645"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1109
                     /locus_tag="ACZO9R_05960"
     CDS             <1..1109
                     /locus_tag="ACZO9R_05960"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR0912644.1"
                     /translation="NYDNQLMSLLTDMDILQSDMDTQEQEIEQANADLENAKAEEQQQ
                     YDAMKQRIQYMYENGDQSFLTALVGADSITDLLNRVEYVSDVYQYDRDMLTNYQEVVQ
                     QVEDLTAQLESEMAEIEELQISYQEQQSSLEQVIATKRSEMADFDSQLANAQSLASQY
                     AQTIKQQNQIIATEKAKQAAAEAAKKKAAQAAAAAGTTGSTTGTDASTAGTGTTSNTT
                     GNTASTGTDTSTSGSSTSGSSSTGLTDGGLNPSYTTGVSGSDVVSYASQFLGNPYVLG
                     GTSLTSGTDCSYFVMAVYQHFGISLPRSSYSQSGCGQAVSYENAQPGDIICYPGHVAI
                     YIGGGRIIHASNPRTGICYGNATYRTITSVRRVL"
     gene            1178..1618
                     /locus_tag="ACZO9R_05965"
     CDS             1178..1618
                     /locus_tag="ACZO9R_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR0912645.1"
                     /translation="MQFKQLQDDMIAAMKARDKVRKDAISTLVSAAKKVAIDEGCRDD
                     IREELVDRVILKEIKSVKEQIDTCPASREDLLAEYKTRYDIFMEYAPKMMSADEVEAF
                     ITEKFADVVASHNKGQIMKAVMPELKGKAEGSVINQVVAKLCQA"
     gene            1615..2268
                     /locus_tag="ACZO9R_05970"
     CDS             1615..2268
                     /locus_tag="ACZO9R_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912646.1"
                     /translation="MRQKLRFTAVLVVLLIILGTASYREQGKRQKLSFPESLDLVVVA
                     VDGEQVTLREMTFYIAYEEGSMEDAAIIYNPDNTDEYWQLYINHTFLREQAKQTVVDM
                     TIHDTLFYQMATAEKITLSEEEEQYLANDQQDFWSDLTEEQRARLGVDEETIDASMRK
                     MALAQKYQSILAEMEQVDYEDYSIGGTEYEKLLAQHTCELDETTWDRVPFGSITVDH"
     gene            2391..>3256
                     /locus_tag="ACZO9R_05975"
     CDS             2391..>3256
                     /locus_tag="ACZO9R_05975"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HIR0912647.1"
                     /translation="MSVKIGRNDPCWCKSGRKYKACHQAFDEKIAAIAAQGHIVPTHD
                     IIKNADQIAGIKESCKINIAVLDYIQEHIHEGMNTAEIDKIVYDMTTSMGGIPAPLHY
                     QGYPYSVCTSVNDQVCHGFPSKDVILKSGDIINVDVSTILNGYFSDSSRMFCIGDVSP
                     EKKRLVDVTKECVEKGLAEVKPWGFLGDMGQAVHDHAFANGYTVVREIGGHGVGLEFH
                     EEPWVGYNSKRGQEMLMVPGMIFTIEPMVNMGKVDIYVDDANDWEVYTEDGLPSAQWE
                     IVVLVTEDGHEVL"
BASE COUNT          904 a          687 c          979 g          686 t
ORIGIN      
        1 ataattatga caatcaactc atgagcctgc ttaccgatat ggacatcctg cagagcgata
       61 tggatacgca ggagcaggag atcgaacagg caaacgcgga tctggaaaat gcgaaagcgg
      121 aggaacagca gcagtatgac gccatgaagc agcggattca gtatatgtat gagaatggag
      181 accagtcctt tttgacggcg ttagtcggcg cggacagcat tacggatctt ttgaaccggg
      241 tggaatatgt ctcggacgtc taccagtatg atcgcgatat gctgacgaac tatcaggaag
      301 tggtgcagca ggtggaagat ctgacggcac agcttgagag tgagatggcg gagatagagg
      361 agcttcagat cagctatcag gagcagcaga gctctctgga gcaggtgatt gcgacaaagc
      421 gttcggagat ggcggatttt gacagccagc ttgccaatgc acagagtctt gcaagccagt
      481 atgcgcagac gatcaagcag cagaaccaga tcatcgcgac ggagaaggca aaacaggcgg
      541 ctgcggaggc ggcgaagaaa aaggcggcgc aggcggctgc tgcggcagga acgaccggca
      601 gcaccaccgg aacggatgcc tcgacagccg gcaccggaac gacctcgaat acgaccggaa
      661 ataccgcttc gaccgggacg gatacctcga cttcgggcag cagtacctcc ggcagctctt
      721 ccacaggtct gacggacggc ggactaaatc cgtcctacac gaccggagtc agcggcagtg
      781 acgtcgtgtc ctatgcgagc cagtttctgg ggaatcccta tgtccttgga ggtaccagcc
      841 tgacatccgg aacggattgc tcctattttg tcatggcggt ctaccagcat tttggcattt
      901 ccctgccgcg atcttcctat tcgcagagcg gctgtggaca ggcagtcagc tatgaaaatg
      961 cacagcccgg cgatattatc tgctatccgg gacatgtggc aatctatatt ggaggaggac
     1021 ggattatcca tgcgtccaac ccgagaaccg gtatctgcta cggaaatgca acttaccgta
     1081 cgatcacttc ggttcggcgt gtgttgtagg tttgtatttt caaaagtaaa atgctataat
     1141 ctggatagaa atgaaataag agatggagga atagaaaatg cagttcaaac agttacagga
     1201 tgatatgatc gcagcaatga aggcaagaga caaggtgcgc aaggatgcaa tctccacact
     1261 ggtgtcggct gcgaagaagg tggcaatcga tgagggctgc cgcgatgaca ttcgggaaga
     1321 acttgtggac cgggtcattt taaaggagat caagtccgtg aaggaacaga tcgatacctg
     1381 cccggcatcc agagaagatc tgcttgcaga atataagacg cgttacgata tttttatgga
     1441 atatgcgccg aaaatgatgt cggctgacga ggtggaggcg tttatcacag agaagttcgc
     1501 ggacgtggta gcaagccaca acaaaggaca gatcatgaag gctgttatgc cggaactgaa
     1561 aggcaaggcg gagggcagcg tgatcaatca ggtggtagca aagctttgtc aggcatgaga
     1621 cagaaattaa gatttaccgc tgttctggtc gttctgctca tcattctggg gacagcatcc
     1681 taccgcgaac agggaaagcg gcagaagctg tcattcccgg aatcgctgga tcttgtggtg
     1741 gtggcagtgg acggggagca ggtgactctg cgggagatga cattttatat cgcatatgag
     1801 gaaggcagca tggaagatgc ggccatcatc tataatccgg ataatacgga tgaatactgg
     1861 cagctgtata tcaatcatac gttcctgcgg gagcaggcga aacagacagt ggtggatatg
     1921 accattcatg atacgctgtt ttaccagatg gcaacagcag agaaaatcac gctctcagag
     1981 gaggaggaac agtatcttgc caatgatcag caggattttt ggagtgacct taccgaggaa
     2041 cagagagcgc gcctgggtgt cgatgaggaa acgattgatg catccatgcg aaaaatggcg
     2101 ctggcgcaga aatatcagag tattcttgcg gagatggaac aggtcgatta cgaggattat
     2161 tccatcggcg gcacggaata cgaaaaactt ctggcacagc atacctgtga gttagacgag
     2221 actacctggg accgcgtgcc gttcggcagc atcacggtag atcattgaac aaagagggaa
     2281 agacagagat tttccattgc gtgaagcagg atatgcgccg atatgtgaag aaagcatttg
     2341 cggcaggcag aatataatat aaaaaataaa atagagaaga gaagaagaat atgtcagtaa
     2401 aaattggaag aaatgatccg tgctggtgca aaagcggccg gaaatacaag gcatgccatc
     2461 aggcatttga cgagaagatt gcagcgatcg ctgcgcaggg acatatcgtc ccgacccatg
     2521 acattatcaa gaatgcagat cagattgcag ggattaagga gagctgcaag atcaatatcg
     2581 cggtgctcga ttacattcag gagcacattc atgagggtat gaatacggcg gaaatcgaca
     2641 agattgtcta cgacatgacg accagtatgg gtggtattcc ggcgccgctg cattatcagg
     2701 gctacccgta cagcgtgtgc acttcggtga acgatcaggt gtgccatgga ttcccgtcaa
     2761 aggatgtcat tttgaagtcg ggagatatta tcaatgtgga tgtttccacg attttgaacg
     2821 gttatttttc cgattcttcc agaatgttct gcatcggcga cgtcagcccg gagaagaaac
     2881 gtctcgtgga tgtcacgaag gagtgtgtgg aaaaaggtct cgcggaagta aaaccgtggg
     2941 gattcttagg cgatatgggt caggcggttc acgatcacgc cttcgcaaac ggttatacgg
     3001 ttgtgcgtga gatcggcgga cacggcgtgg gactggagtt ccatgaggag ccgtgggtcg
     3061 gctataacag caagcgcggt caggagatgc tgatggtgcc gggtatgatt tttacgatcg
     3121 agccgatggt caacatggga aaagtggaca tctatgtcga tgatgcaaac gactgggagg
     3181 tctacacgga ggacggactt ccgtctgcac agtgggagat tgtggtgctt gtgacggagg
     3241 acggccacga ggttct
//
LOCUS       DBMDQJ010000241         3254 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10696_length_3254_cov_1.967333, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000241 DBMDQJ010000000
VERSION     DBMDQJ010000241.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10696_length_3254_cov_1.967333"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            95..1273
                     /locus_tag="ACZO9R_05980"
     CDS             95..1273
                     /locus_tag="ACZO9R_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR0912648.1"
                     /translation="MSSSGNSRNGAHSSNGAHSSNRIQTGGSGHPQKKPLTKAQKAAI
                     RKKKRQKKIILVVVEILVLLLLAVVLFAVVKLSKIEKDTSFNESDLEFNEGLSSESQQ
                     IMKGYTTIALFGLDNRSNGNLSKGNSDVIMIASINNDTHEVKLVSVYRDSYLDIGNST
                     YRKCNSAYANGGPEQAISMLNTNLDLNITDYVTVDFNAVVECVDLLGGVEMTVTDEEA
                     VLMHGYMDEISKLTKKKSEYLPGAGTYTMDGVQACAFARIRYTAGDDYKRTERQRAVL
                     TAMIQKAQKSDLKTINSLIDEVFGDIKTSYSNTDLIALASQVFNYSLGETTGFPFEKN
                     TVTLGSKGSVVVPCDLKSNVIQLHQFLYDDTEYEPTDTVKSNSEKIISDTGFHQGDGY
                     "
     gene            1273..2256
                     /locus_tag="ACZO9R_05985"
     CDS             1273..2256
                     /locus_tag="ACZO9R_05985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912649.1"
                     /translation="MRVDIIIPTYKPDDTFCLLLQKLQEQTFVIHELILVNTEEALWN
                     EAVRKYPIEQIRKELPFSSRIFHMTRENFDHGGARNMGAGYSEADILIFMTQDAVPAD
                     ERLVEHLVHALTSGAHGPGTPAKGAVAVAYARQLPREDCGIIERYTRQFNYPDEGCVK
                     SREDIPRLGIKTFFCSDVCAAYRRDLFEQLGGFESPVIFNEDMFFAAHAVQEGYKVAY
                     AADAEVVHSHNYSVKQQFHRNFDLAVSQTAHPEIFEQVSSEAEGMRLVKNTMGYLCKI
                     HKPYLIWKLGWQCVGRYAGYFLGKRYQKLGRRQILKCTMSPYYWKRIWSEK"
     gene            2267..3247
                     /locus_tag="ACZO9R_05990"
     CDS             2267..3247
                     /locus_tag="ACZO9R_05990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912650.1"
                     /translation="MSNILYIVVPCYNEQEVLPETSKRLREKVQTLRQGGKIDADSRI
                     LFVNDGSKDATWEMIQKLHEENSVFSGVNLSRNRGHQNALLAGIAVAAEHADMIVSMD
                     ADLQDDVDAMDRMIDAYEAGNDIVYGVRSSRQKDTFFKRNTAQLFYRMLHFLGAEIVY
                     NHADYRLLSRRAAKGLLSFEEVNLFLRGIVPMVGYQSTTVTYERGERFAGESKYPLGK
                     MVAFALEGVTSLSVKPIRMITMGGLFVCLVSLGMLIKAFVDYANGNVEPGWTSVIISI
                     WVLGGLQLFAIGIIGEYIGKTYMETKRRPRYLIESVLLDAVEQEDTPGRE"
BASE COUNT          850 a          690 c          961 g          753 t
ORIGIN      
        1 tcgagaactg gacactggga ctggatgtca agattctgtt cctcacggtt ttcaagggat
       61 ttatcaataa aaatgcatat taggagaaaa atttatgagt agtagtggca acagcagaaa
      121 tggggcacat agcagcaacg gggcgcacag cagcaaccgg atacagacag gcggcagcgg
      181 acatccgcag aagaagccgc tgacaaaagc acagaaggca gcgatccgca agaagaagcg
      241 tcagaagaag attattctcg ttgtggttga gatcctggta cttctgcttc tggcagttgt
      301 attgtttgcg gtagtgaagc tctccaaaat cgagaaggac acctccttca atgagagtga
      361 tctggaattc aacgagggac tttcttcgga atcccagcag atcatgaagg ggtatacgac
      421 gatcgcgctg ttcggattgg acaaccgttc caacggcaac ttatccaagg gtaacagcga
      481 cgtgattatg attgcgagca tcaacaacga tacacatgag gtgaagctgg tatccgtata
      541 cagagattcc tatctcgata tcggcaacag cacctaccgg aaatgtaact cagcctatgc
      601 gaacggcgga ccggagcagg cgatctccat gttaaacacg aaccttgact taaatattac
      661 ggattatgtg accgtggact ttaatgcggt ggtagagtgt gtggatcttc tcggcggtgt
      721 tgagatgacc gtaaccgatg aggaagcggt gctgatgcac ggctacatgg atgagatctc
      781 caagctgacc aagaagaaat ccgagtatct gccgggagcc ggcacctaca caatggatgg
      841 tgtacaggca tgtgcattcg cgcgtatccg ttatacggcg ggcgatgact ataagcgtac
      901 cgagcgtcag agagcagtgc ttacggcaat gatccagaag gcgcagaagt ccgatctcaa
      961 gacgatcaac agcctgatcg atgaggtatt cggcgatatt aagacaagct acagcaacac
     1021 cgacctgatt gcacttgcat cccaggtatt caattacagc cttggcgaga cgaccggatt
     1081 cccgtttgag aagaatacgg tcacgctcgg ttccaaggga agtgtggtag taccgtgtga
     1141 tctgaaatcc aacgtcattc agctgcatca gttcctgtac gatgatacgg aatatgagcc
     1201 gacagacacg gtaaagagca acagtgaaaa gattatcagt gataccggat tccaccaggg
     1261 agatggctat tgatgcgtgt agatatcata attccgactt acaaaccgga tgacactttt
     1321 tgtctgctcc tgcaaaaatt gcaggagcag acctttgtta tacatgaact tattctggtt
     1381 aataccgagg aagcactgtg gaatgaggct gtacggaagt atccgatcga acagattcgg
     1441 aaggagcttc cgttttctag ccgcattttt cacatgacca gagagaactt tgaccacggc
     1501 ggtgccagga atatgggagc ggggtattcc gaagcggata ttctgatatt catgacacag
     1561 gatgcggtgc cggcagacga aaggctggtg gaacatctgg tgcatgcgct cacatccggt
     1621 gcgcacggtc cggggactcc agccaaaggt gcggttgcgg tcgcgtatgc aaggcagctc
     1681 ccgcgggagg actgcggcat catcgagcgc tataccagac agtttaatta tcccgatgag
     1741 ggatgcgtca agagccgtga ggacataccg cggcttggca ttaagacttt tttctgctcg
     1801 gacgtgtgcg ctgcataccg cagggatctg tttgagcagc tcggcggatt tgaatcgccg
     1861 gttattttta acgaggatat gttttttgcg gcgcatgcag tgcaggaagg atacaaggtt
     1921 gcgtatgcgg cggacgccga ggtggttcat tcgcacaact attcggtgaa gcagcagttt
     1981 caccgcaatt ttgatctggc ggtgtcccag acggcacatc cggagatctt tgagcaggtc
     2041 agctcggagg cggagggcat gcgtctggtg aaaaatacga tgggttatct gtgtaagatt
     2101 cacaagccgt atctgatctg gaagttaggg tggcagtgtg tcggcaggta cgccggctat
     2161 tttctgggaa aacgctatca gaagcttggc agaagacaga tcttgaagtg taccatgagt
     2221 ccgtattact ggaagcggat atggtcagaa aagtagggag cagaaaatga gtaacatact
     2281 atatatcgta gtaccctgct ataatgagca ggaggtactt ccggagacat ccaaaagact
     2341 gcgggaaaaa gtgcagacat tacgtcaggg cggcaaaatc gacgcggaca gccggattct
     2401 gtttgtgaat gacggatcga aagacgcgac gtgggagatg attcagaagc ttcacgagga
     2461 gaattccgta ttttccggtg tcaatttatc caggaaccgc ggacatcaga atgcgcttct
     2521 ggcagggatt gcggtggcgg cggaacatgc ggatatgatc gtctccatgg atgcggatct
     2581 gcaggacgat gtggatgcga tggaccgcat gatcgatgcc tatgaagcgg gaaatgatat
     2641 tgtctatggc gtgcggtctt ccagacagaa ggacacgttt tttaagcgca acacggcaca
     2701 gcttttttac cggatgcttc atttcctggg ggcggagatc gtgtacaatc acgcggatta
     2761 ccggctgtta agcagacgtg ccgcaaaagg actcctgtct tttgaagagg tgaatctgtt
     2821 tctgcgtgga attgttccaa tggtgggcta tcagagtacg accgtaacct atgagcgcgg
     2881 ggagcgtttc gcgggggaga gcaaatatcc gcttggaaaa atggtggcat ttgcgctgga
     2941 gggagtgaca tcgctctcgg tcaagccgat ccgcatgatt acgatgggcg gattgtttgt
     3001 gtgcctcgtc agcctgggaa tgctgattaa ggcgtttgtg gattacgcga acggcaatgt
     3061 ggaaccgggg tggacaagtg tgattatttc catctgggtg ctcggcggtc tgcagctgtt
     3121 tgcgattgga attatcggag agtatatcgg gaagacgtat atggagacga aacggcgtcc
     3181 gagatatctg attgaatcgg tgcttttgga tgcagtagag caggaagaca ctcccggcag
     3241 ggaataacgg tttt
//
LOCUS       DBMDQJ010000242         3245 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10734_length_3245_cov_1.751908, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000242 DBMDQJ010000000
VERSION     DBMDQJ010000242.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10734_length_3245_cov_1.751908"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="ACZO9R_05995"
     CDS             complement(<1..449)
                     /locus_tag="ACZO9R_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912651.1"
                     /translation="MGRQFDQMEPAVRAHMLDSLEVAVENVPDWMKIHFLSFCMAQKR
                     DPHITEKLLDTVLAADYSEVGEYNKLSHYWQITRAVFTDGTLKSSEVERKLTGLYHTL
                     FEAFSTAVGVTKRCYIPRKERNQNLVIVFSPQVLGLGHAPTKTLLDR"
     gene            complement(546..2909)
                     /locus_tag="ACZO9R_06000"
     CDS             complement(546..2909)
                     /locus_tag="ACZO9R_06000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0912652.1"
                     /translation="MEQRNIVLVSGSGVYGAVSKYITEFAAAFRELGYHTVILDGNLK
                     SFRDKYRYLKEHISIYALVDCQAMVADVLPECLEDSSVPRVHYLCDHPLYLYERLERL
                     NESDIILNVDARHTAYLKKYYPRLERVAYVPLSGTGADIQKPYQNREIGLLFTGSYWV
                     PQMPVHKTAEMGFADSVKWSVQTMLTGNPYLSVEDALEKVLESCQVTVGREEFAAILS
                     ELSGVEFYAREYYRDRMIRTLLKAGVDVWVYGNGWEKLLCPGREHLHVMDGGAEVARR
                     ALGEAKIVLNIMPGFKAGFQERIAAAMLSGAVAVTDISDYLKENFSNGKEMVFYELDH
                     LEELPSIIQSLQENPVRSEKIAENGKRVAQKQHTWMQRVIQMAEQIEVYHGKTADFEA
                     NAGGELTVPLCELRESYVVEEIGVRLSEEISRLNDLEHCGCAVQEDVDRLTGYLREWE
                     KQLRERCGYRFFSGNNLEVFLADCGSVRQLLLMADGMLCGIREKYDAAALEEFASGLG
                     AGENRLLYQKAVTRLFKERYKEKEDPWIKLWKQDLEAGTILQSCPGAVCARYEQAVSG
                     LLYDASCDMFYVMIDGKPLYYPQEFSDEQVLERYRKLCEEQDRESAHCYRNGNLQIPD
                     GAVIMDAGAAEGYFALTVIEHASRVYLTDRDERWNAAWQKTFAPYRDKVVFLHGVLGG
                     SEAECDVMTIDDIIGTGRLDFLKVDVGGAEQEVLLGAQKVLGNAAAMRCVVAAYHTHE
                     SEAQIEHILRENGFQTDRADGYFLHMDYEKPVWENELRHALVWAERN"
     gene            complement(2941..>3245)
                     /locus_tag="ACZO9R_06005"
     CDS             complement(2941..>3245)
                     /locus_tag="ACZO9R_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR0912653.1"
                     /translation="PSFKITDLAQAILPGCEMPEVGIREGEKLHEIMVTREDSMMTYE
                     YEKHFIVYPHFDWWSNTKIQAGGKLVEPGFEYSSGTNTEWLSVEQIQERLETVKEH"
BASE COUNT          766 a         1004 c          600 g          875 t
ORIGIN      
        1 cttcgatcca gcagcgtttt cgtgggcgca tgtccaaggc ccagtacctg cggtgaaaac
       61 actattacca gattctgatt tcgctctttc ctcggaatat agcaccgctt tgtcactccc
      121 accgcggtcg aaaatgcctc aaacagtgta tggtataatc cggtaagctt tctttccacc
      181 tcagaacttt ttaaggttcc gtctgtaaat accgctcttg tgatctgcca gtaatgactg
      241 agtttattgt actccccgac ttcactgtaa tctgccgcaa gcaccgtatc caacaatttt
      301 tccgtgatat gtggatcccg cttttgcgcc atacaaaaag agagaaaatg aatcttcatc
      361 cagtccggca cattttcaac cgctacttcc agagaatcca gcatatgcgc ccgcaccgcc
      421 ggctccatct ggtcaaattg ccgtcccaaa tgttcaaggg ttgtatcaat ctctggatac
      481 ttttcaaaat atgtagcgca atcatatgca gcaatctgtg tcctatattc ttcaaatgtc
      541 atccgtcagt tcctctccgc ccacaccagc gcatgccgca gctcgttctc ccataccggt
      601 ttttcgtaat ccatatgtag aaaatacccg tctgccctgt ccgtctgaaa gccgttctct
      661 cttaaaatat gctcaatctg tgcttcactc tcgtgtgtat gataggcagc aaccacacac
      721 cgcatcgccg cggcattccc cagaaccttc tgtgctccca gcagaacttc ctgctccgca
      781 cctccgacat ccaccttcaa aaaatccagt ctgccggttc cgatgatatc atcaatcgtc
      841 atgacatcac attcggcttc tgatccgcca agcacaccgt gtaaaaacac caccttatcc
      901 cgatatggtg caaatgtctt ttgccatgca gcattccagc gttcatcccg gtctgtcaaa
      961 tataccctgc ttgcatgttc tatcacggta agcgcaaaat atccctcagc tgctccggca
     1021 tccataatta ccgctccgtc cggaatttgc agattcccgt tcctgtagca gtgcgccgac
     1081 tctctgtcct gttcctcgca aagtttccga tatctctcca gaacctgctc gtcggaaaat
     1141 tcctgcggat agtataacgg ctttccatca atcatcacat aaaacatatc gcaggaagca
     1201 tcatacagca gcccggagac cgcctgctca tatctggcgc agaccgctcc cggacaagac
     1261 tgcagaattg tccccgcttc cagatcttgt ttccacagtt ttatccacgg gtcttccttc
     1321 tccttgtatc tctccttaaa aagccgggtc accgcctttt gatataacag tcggttctca
     1381 cctgcaccaa gcccggatgc aaactcctcc agcgcagccg cgtcatattt ttcccggata
     1441 ccgcacaaca taccatctgc cattaacagg agctgccgca ccgagccaca gtccgccaga
     1501 aatacttcca gattatttcc cgaaaaaaag cggtacccgc accgttctct cagctgtttc
     1561 tcccactccc gcagataccc tgtcagtctg tcaacatcct cctgcaccgc acagccgcaa
     1621 tgttccaaat cgttgagccg gcttatctct tcgctcagtc gtacaccgat ctcctccacc
     1681 acgtaagact cacgtagctc gcacagcgga accgtcagtt cgcctcctgc atttgcttca
     1741 aagtccgctg tttttccgtg atatacctcg atctgttctg ccatctgtat cacgcgctgc
     1801 atccaggtat gctgtttctg tgccacgcgc tttccatttt ccgcaatttt ttcactgcgt
     1861 acgggattct cctgaagact ctgtataata ctgggaagtt cttccagatg atccagttca
     1921 tagaatacca tttctttgcc gttggaaaaa ttctctttca gataatcgct tatatccgtc
     1981 acggcgaccg ctccgcttaa catggcagcc gcaatgcgct cctggaatcc cgccttaaat
     2041 ccaggcataa tgtttagaac aatctttgcc tctccgagtg ctctgcgtgc cacctcggca
     2101 ccaccatcca tcacatgcag atgctctctg cctggacaca atagcttctc ccatccgttg
     2161 ccgtagaccc agacatccac accggctttc agcagggtgc gaatcatcct gtcccggtaa
     2221 tattcccttg cataaaactc caccccggaa agttcactta aaatagcagc aaattcttcc
     2281 ctgcccaccg taacctgaca gctttccagc actttctcca aagcgtcctc tacagaaagg
     2341 tacggattac cggtcagcat ggtctgcacc gaccacttta ccgaatccgc aaatcccatc
     2401 tccgcggtct tgtgcacagg catctgcggc acccagtagg aaccggtaaa aagcagccct
     2461 atttcccggt tctgatatgg tttttgtatg tctgcccctg ttccgctcag cggaacataa
     2521 gcaacacgtt ccagacgcgg ataatacttt ttcagataag ccgtatgcct cgcatccaca
     2581 ttcagaataa tatcactttc attcagacgt tcaagccgtt cgtacaggta cagcggatga
     2641 tcgcacagat aatgcacccg tggaactgaa gaatcctcta agcattccgg aagtacatct
     2701 gcaaccatcg cctgacagtc taccagcgcg tagattgaaa tgtgttcttt cagataccgg
     2761 tatttgtcac gaaatgattt gagatttccg tccagaatca cggtatgata cccaagctca
     2821 cgaaatgctg ccgcaaattc tgtaatgtat ttgctgaccg ctccatacac accgcttcca
     2881 ctgaccagta caatatttct ctgttccatc cctgtctcca ttccggagtg tttctaccca
     2941 ctaatgttct ttgactgttt ccaaccgctc ctgaatctgc tctacgctca gccactctgt
     3001 attggtaccc gagctatact caaatcccgg ttctacaagt ttgccgcctg cctgaatctt
     3061 ggtattgctc caccagtcga aatgcggata cacaataaaa tgcttctcgt attcatatgt
     3121 catcatggaa tcctcacgtg ttaccatgat ctcatgcagc ttttctccct cacggattcc
     3181 tacctccggc atctcgcatc ccggaagaat cgcctgtgcc agatcagtaa tcttaaaaga
     3241 cggaa
//
LOCUS       DBMDQJ010000243         3218 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10844_length_3218_cov_1.427013, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000243 DBMDQJ010000000
VERSION     DBMDQJ010000243.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3218)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3218)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3218
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10844_length_3218_cov_1.427013"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2425
                     /locus_tag="ACZO9R_06010"
     CDS             <1..2425
                     /locus_tag="ACZO9R_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912654.1"
                     /translation="KEATLPVITMYQGDQELNELHGYVTEMDAAYMRDTITTVGEDRK
                     LPIVIQTNQTAVDTISYEIRSLDATRLIANADVTSYEERSGKITADLQIQNLLEEGEE
                     YLMIIRLGSGDRSICYYTRIAEPVDCYVSECVAFVKDFNDKTFNDETSGTLATYMERT
                     TGDNTTLQFVSLNSSLKQVSWADFKGERLTEPVPSIKEITPTYNVIVLDYVVSSVNES
                     GQIEYYNIEEYYRVRYTTSRIYLLNFERTMNQIFRGENDSVYDQYIQLGIREANVEYK
                     ANEAGTSVAFVQEGELWSYNTVENTLAKVFSFRGYEGIDSRENYGEHDIKIVGVDEAG
                     SVDYIVYGYMNRGIHEGEVGIAVYHYDSLANTDEELVFIPSDKSYEVMKSELGQLMYV
                     NEGGELFLMLDGCVYGIDLNTLSVRELVTNLSVQDFAVSASNRYFAWMETEVDSQMGS
                     SVIHVMNLSDQSTQEIAAGTGEAIKPLGFMDEDFVYGIARQSDIFEDAAGNVTFPMHQ
                     IRIVDVSTKDLIVQKTYEKSGYFVSSVSIEDYTMHLNRIQYNGTAYVPADQDMIMNRE
                     GDSGKLVNIATSSSAEKETQVQLTIAGASAEKAPKLLTPKETILEEERTVAIKEEGST
                     KHYYVYVKGNVVLATDDVTEAVTAANDSMGVVIGDNLQYVWKRSRKSAQPALSDIAVG
                     AEDAGAGSIAQCINAMLEKEGINISVNALIEGGETPKSILNNTMKDMQVLDLTGCNIE
                     EVLYYVSCGSPVFSMTGSTDAVLIIGYDSNSVVIFDPALGTTYRKTLTEADEMFTAAG
                     NVFFTYLVK"
     gene            2561..2815
                     /locus_tag="ACZO9R_06015"
     CDS             2561..2815
                     /locus_tag="ACZO9R_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR0912655.1"
                     /translation="MSRKEIVVSNVSEEHSNPIAELVQVACRFDSDITLESNNRRINA
                     KSIMGIMAFNPSKGMTVNIVAEGSDEEEALVAMEKFLVCE"
     gene            2845..>3218
                     /locus_tag="ACZO9R_06020"
     CDS             2845..>3218
                     /locus_tag="ACZO9R_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6465 family protein"
                     /protein_id="HIR0912656.1"
                     /translation="MEKKTTAKLDETKAAVKAAEAKVAAKVEETKTAAKEATAKVVAK
                     VEETKNAAKETEVKAATKVEEPKKTAAETAKKRKPRTKTAKKAAKEELKPEVYIQYQG
                     REGVVEDAIKKVTEQFVAEGHRA"
BASE COUNT          945 a          632 c          968 g          673 t
ORIGIN      
        1 gaaggaggcg acgctgccgg tcatcaccat gtatcagggc gatcaggaac tgaatgaact
       61 acacgggtat gtgacggaga tggatgccgc atatatgcgc gataccatta cgacggtggg
      121 ggaagaccgg aagcttccga ttgtgataca gacgaatcag acggcggtcg acacgatttc
      181 ctatgagatc cgcagtctcg atgccacacg cctgattgcg aatgcggatg tcacctccta
      241 tgaggagcgc agcgggaaga tcacggcaga tctgcagatc cagaatctgc tggaagaggg
      301 cgaagagtat ctgatgatta tccgcctcgg gagcggagac cgttcgatct gttactatac
      361 caggatcgcg gagccagtcg actgctatgt gagcgagtgc gtcgcattcg tcaaggattt
      421 taacgataag acgttcaacg atgagacctc cggaacactg gcgacttaca tggagcggac
      481 gaccggggat aacacgacgc tgcagttcgt gtcgttaaat tcttctctaa agcaggtgag
      541 ctgggcggat tttaagggag agcgtctgac agagccggtt ccgtcaatca aggagatcac
      601 accgacctac aacgtgatcg tgctcgatta tgtggtatcc agtgtcaacg agagcggtca
      661 gattgaatac tataatatag aagaatatta cagggtgcgc tacaccacaa gccgcatcta
      721 tctgctgaat ttcgagcgaa ccatgaacca gattttccgc ggagaaaatg acagcgttta
      781 cgatcagtat atccagcttg gaatccgcga agcgaacgtt gaatacaagg cgaatgaggc
      841 gggaaccagt gtggcgttcg tgcaggaggg agaactctgg agctacaata cggtggaaaa
      901 tacgcttgca aaagtgttca gtttccgcgg ttacgaggga attgacagcc gcgagaacta
      961 cggagaacac gatatcaaga tcgtcggtgt ggacgaagcc ggaagcgtgg attatatcgt
     1021 gtacggttac atgaaccgcg gaattcatga gggggaagtc ggaattgcgg tctatcatta
     1081 cgacagcctg gcgaataccg atgaggaact ggtcttcatt ccgtcggata agtcgtatga
     1141 ggtgatgaag tcggaactcg ggcagctcat gtatgtaaat gagggcggag agcttttcct
     1201 gatgctggac gggtgtgtct acggaatcga tttaaatacc ctctcggtgc gggaactggt
     1261 gacgaatctg agcgtacagg attttgcggt gtccgcgtcc aaccgctatt ttgcatggat
     1321 ggagacggag gtggattccc agatgggcag cagtgtgatc cacgtgatga atctgtcgga
     1381 tcagagcacg caggagattg cggcgggcac cggggaagcc atcaagccgc ttggcttcat
     1441 ggatgaggat tttgtgtatg gcattgccag acagtcggat attttcgagg acgccgccgg
     1501 aaatgtgaca tttccgatgc accagatccg gattgtggat gtctcgacaa aagatcttat
     1561 tgtgcagaaa acatatgaaa agagcggcta ttttgtaagc agcgtgtcca tcgaggacta
     1621 cacgatgcac ttgaaccgca tccagtataa cggcacggca tatgtcccgg cggatcagga
     1681 tatgatcatg aaccgtgagg gagactccgg aaagcttgtc aatattgcaa cgagcagttc
     1741 tgcggaaaag gagacccagg tacagcttac gattgccggg gcatccgcgg aaaaggcgcc
     1801 gaagcttctc accccgaagg agacgatttt agaagaggag cgcacggtgg caatcaagga
     1861 agagggcagc acaaagcatt attatgtata cgtgaagggg aatgtggtgc tggcgacaga
     1921 tgatgtcaca gaggccgtca cggcggcaaa tgacagtatg ggagtcgtga ttggagataa
     1981 ccttcagtat gtgtggaaac gctccagaaa gtccgcacag ccggcactgt cggatattgc
     2041 ggtcggtgcg gaggatgccg gagcaggaag cattgcgcag tgcatcaatg ccatgctgga
     2101 aaaagaagga atcaatatca gcgtgaacgc actcattgag ggcggagaga cgccgaaaag
     2161 catcctgaac aatacgatga aggatatgca ggtgctggat ctgaccggat gcaatatcga
     2221 ggaggttctc tactatgtca gctgcggcag tccggtattt tccatgacag gaagcacgga
     2281 tgcggtgctg attatcggat atgacagcaa cagcgtcgtg atatttgatc ctgcactggg
     2341 aacgacatac cggaagacgc tgaccgaggc ggatgagatg tttacggcgg caggaaatgt
     2401 atttttcaca tatctggtga aatgaaagtg cataaaaaag ctcttaaagt aggaaattat
     2461 tcgtgtaagt ttaacagatt tcatgttctt ctattgcaaa ttggaaatag atgggttata
     2521 ttttgagacg tagtacttga aaagaggaaa tggagagatt atgagcagaa aagagattgt
     2581 tgtatcgaat gtctcagagg aacatagtaa tccgattgca gaactggttc aggttgcctg
     2641 cagatttgac agtgatatta cacttgagag taacaaccgc cggataaatg cgaagagtat
     2701 tatgggaatc atggcattta acccgtccaa aggaatgacg gtgaacatcg ttgccgaggg
     2761 cagtgatgaa gaggaagcat tagtagcaat ggaaaagttt ttagtatgtg agtagttttt
     2821 ttgatgagga ggagggctct cataatggaa aaaaagacga cagcgaaact tgatgagact
     2881 aaggcagcag taaaggcagc agaagcaaaa gttgcggcga aggttgagga gaccaagaca
     2941 gcagcgaagg aagcaactgc aaaggttgtg gcaaaagtcg aggagacgaa gaacgcagcc
     3001 aaagagacag aggtaaaggc ggctacaaag gtagaagaac cgaagaagac agcagctgag
     3061 accgcaaaga aaagaaaacc aagaacaaag acagcgaaga aggcagcaaa ggaagagctt
     3121 aagcctgaag tgtatatcca gtatcaggga cgtgaaggcg tggtagagga tgccattaag
     3181 aaagtgacag agcagtttgt ggcagaaggt cacagagc
//
LOCUS       DBMDQJ010000244         3216 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10856_length_3216_cov_1.263563, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000244 DBMDQJ010000000
VERSION     DBMDQJ010000244.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10856_length_3216_cov_1.263563"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="ACZO9R_06025"
     CDS             complement(<1..1046)
                     /locus_tag="ACZO9R_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR0912657.1"
                     /translation="MGHIQTQEEWEVQMAEKILSYVRNELYLELRYLDVAFSALVPQA
                     DASLQSFATDGGHLFYSTEQILRVFEKNAPYLNRAYLHTVLHCIFSHPWIAGNRDRRL
                     WNLACDVAVEYTIDGLGKKCTKRILSWTRQKLYEELREQKKGVSAAVVYDLLWERYFP
                     LPAEGMPVCEEWQALAREFYTDSHKYWPKREDDAAKCAAAADNQKKWNQIARQTRMEQ
                     ERRGEKPKDGEDLLAAQLAAGKSRRSYRDFLQKFAVLHEELHADPEEFDLSYYTYGLS
                     VYGNLPLIEPLESREVEKIREFVIVIDTSYSTSGELVEQFLRETANILHQSDSFFARS
                     VIRVLQCDNVVRSDA"
     gene            complement(1120..2223)
                     /locus_tag="ACZO9R_06030"
     CDS             complement(1120..2223)
                     /locus_tag="ACZO9R_06030"
                     /inference="COORDINATES: protein motif:HMM:NF024702.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HIR0912658.1"
                     /translation="MNYKLYWELTNEGDARLLRAFGETPEPRFPAEIEGRAITEIGDY
                     CFAAQAHLPAECRCSYVQAEPGADGAPETAPDGEMQAEPGADGAPETAPDGEMQAEPE
                     TDGAPETDDALAELAGAYITRVTLPEGIKKIGNFAFYNATELAELELGSGIDTLGSDA
                     FMNCRSLSRLLLHADPGQKTGLRLLLAQLSSDLEVTLSGENGVWAKLLFPEYYESYDE
                     IAPAHIFGRNIVGEGFRARQSFREDVLDFAQYDKIFPQACVDESEATLGRLALDRVRY
                     AAELSEAPRGLYEEYLKAHSGYLIRRITDDRDLELAEDCCSRKFLTREDVAACAMRAG
                     EADWAEGAAALLHLMQQYFAEKTPDECYSFDDF"
     gene            complement(2279..>3216)
                     /locus_tag="ACZO9R_06035"
     CDS             complement(2279..>3216)
                     /locus_tag="ACZO9R_06035"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR0912659.1"
                     /translation="NRILFEDPVTSVEVPYTSLLTAAVIDGLLARYPFLKSGEIGKSV
                     MGKSISYLEIGNGEKEVFYNASFHANESITTPVLLKFAEEYAQAYEKGTALYGVPAAE
                     LFEEYRLFLVPLVNPDGVDLVNGLLTDGGYYRRAQQIASDYPAIPFPAGWKANIEGID
                     LNLQFPAGWENAKRIKYAQGYTKPAPRDYVGEVPLTAPESEAVYAFTRSHDFRLILAY
                     HTQGEVIYWKYLDYEPARSYEIAQYFGRVSGYTVEQTPTQSGYAGYKDWFIQEYDRPG
                     YTIETGMGENPLPMSDFAGIYRDNTGILLGGMTQI"
BASE COUNT          638 a         1082 c          735 g          761 t
ORIGIN      
        1 gcatcgcttc gtaccacatt gtcgcactgt aaaacccgga tcacggaacg cgcaaagaaa
       61 ctgtcgctct ggtgcaggat gtttgccgtc tctcgcaaaa actgttcgac cagttcaccg
      121 ctggtggaat agctggtgtc aatcacgatc acaaactcgc ggatcttctc tacctccctg
      181 ctctcaagcg gctcgatgag cggcagattc ccgtataccg aaagtccgta cgtgtaataa
      241 ctcagatcaa attcctcagg gtcggcatgc agttcctcgt gcagcacggc aaatttctgc
      301 aggaaatccc ggtagcttcg tctgcttttc cccgccgcaa gctgcgccgc cagaaggtct
      361 tcaccgtcct tgggcttctc tccccgccgc tcctgctcca tgcgggtctg cctggcgatc
      421 tgattccact ttttctgatt gtccgccgca gccgcacact ttgccgcatc gtcctcccgc
      481 ttcggccaat atttgtgact gtccgtgtaa aattcgcgcg ctaacgcctg ccattcctca
      541 cagaccggca ttccctccgc cggcagcggg aaatagcgct cccacaaaag atcgtagacg
      601 acggcagccg acacgccttt tttttgttcc cgcagttctt cgtacagctt ctggcgcgtc
      661 cagcttaaga tccgtttcgt acattttttt cccagaccgt caatcgtata ttccacagca
      721 acatcacagg cgagattcca cagccgcctg tcgcggttcc ccgcaatcca cggatgggag
      781 aaaatacagt gcagcactgt gtgaagatag gcacggttta ggtacggtgc atttttctca
      841 aacacacgca gtatctgctc tgtggagtag aacagatgtc ccccgtccgt ggcaaagctc
      901 tgcaacgacg catccgcctg cggcacaagc gccgagaatg cgacgtccag ataccgcaat
      961 tccaggtaca gttcgttgcg cacataagaa agtatctttt ccgccatctg cacttcccat
     1021 tcttcctgtg tctgtatatg tcccatgcca tctctcctct tccggtttct ttttcaaaat
     1081 accctgcatc cgcacgtact tttccgtgta aaaacaatct taaaaatcgt caaacgaata
     1141 gcactcgtcc ggcgttttct ccgcgaaata ctgctgcatc agatgaagca gcgccgccgc
     1201 tccctccgcc cagtccgctt cccccgcgcg catggcacac gccgccacgt cctcccgcgt
     1261 cagaaacttc cggctgcagc aatcctccgc cagttccaga tcccggtcat ccgtgatgcg
     1321 gcggatgaga taaccgctgt gcgccttcag atactcctcg taaagtccgc gcggcgcctc
     1381 cgaaagttcc gccgcatagc gcacgcggtc gagtgccagc cgtcccagcg ttgcctccga
     1441 ttcatccaca cacgcctgcg ggaaaatctt atcgtactgt gcgaaatcga gcacgtcctc
     1501 gcggaagctc tgtctcgccc ggaatccctc ccccacaata tttctgccga aaatatgtgc
     1561 cggcgcgatc tcgtcatagc tctcataata ttccggaaac agcagcttcg cccacacgcc
     1621 gttttcgccg cttaaggtca cttccagatc cgacgaaagc tgtgccagca aaagcctgag
     1681 ccccgtcttt tgcccggggt ccgcatgcag taacagccgc gacaggctcc ggcagttcat
     1741 aaatgcatcg ctccccagcg tgtctatccc gcttccaagc tccagttccg ccagctccgt
     1801 ggcattataa aaagcaaagt ttccgatctt ttttatgccc tccggcagcg tcacgcgcgt
     1861 tatgtatgcc cccgccagtt ccgcaagcgc atcgtctgtc tccggtgcgc cgtctgtctc
     1921 cggctccgcc tgcatctccc cgtccggcgc tgtctccggt gcgccgtctg ctcccggctc
     1981 cgcctgcatc tccccgtccg gcgctgtctc cggtgcgccg tctgctcccg gctccgcctg
     2041 cacataactg caccggcatt ctgccgggag atgcgcctgc gccgcaaagc agtagtcccc
     2101 gatctccgta atcgctcttc cctctatctc tgccgggaaa cgcggctccg gcgtctcgcc
     2161 aaacgcccga agcagccgcg cgtctccctc atttgtcagc tcccagtata acttatagtt
     2221 catcttctgc tcctaatgta aactctacgc tccattatac gaaaggcgtt tttctctgtc
     2281 aaatctgcgt cattccgccc agcagaattc cggtgttgtc ccggtagata cccgcgaaat
     2341 ccgacatggg aagcggattt tctcccatcc cggtctcgat cgtgtagccc ggtctgtcgt
     2401 attcctggat aaaccagtcc ttatacccgg catatcccga ctgcgtgggc gtctgctcca
     2461 cggtatagcc gctgacacgc ccaaaatact gcgcaatctc gtaggagcgc gccggttcat
     2521 agtccagata cttccagtag atgacctccc cctgcgtgtg atacgcaagg atcagcctga
     2581 aatcatgtga tctggtaaac gcgtacaccg cctcgctctc gggcgccgtc aggggtacct
     2641 ctcccacata atctctgggc gccggcttcg tgtacccctg cgcatattta attctctttg
     2701 cgttttccca gcctgccgga aactgtagat ttaaatctat gccctcgata ttcgccttcc
     2761 atccggccgg aaacggaatt gccggataat ccgatgcaat ctgctgcgcc cgccggtaat
     2821 acccgccgtc cgtaagcagc ccgttcacca gatccacccc gtcgggattg acgagcggca
     2881 caaggaaaag ccggtactcc tcaaacagtt ccgccgccgg cacgccgtag agcgccgttc
     2941 ctttttcgta cgcctgcgca tattcctccg caaatttgag cagaaccggc gttgtgatgc
     3001 tctcatttgc atgaaaagat gcgttgtaga atacctcttt ctccccgttt ccgatctcca
     3061 gatacgaaat cgactttccc atcacacttt tgccgatctc cccgctcttt aaaaacgggt
     3121 agcgcgccaa aagcccgtca atcactgccg ctgtgagcag cgacgtataa ggcacctcca
     3181 ccgacgttac cgggtcttca aaaaggatcc ggttct
//
LOCUS       DBMDQJ010000245         3210 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10883_length_3210_cov_1.760695, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000245 DBMDQJ010000000
VERSION     DBMDQJ010000245.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10883_length_3210_cov_1.760695"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="ACZO9R_06040"
     CDS             <1..425
                     /locus_tag="ACZO9R_06040"
                     /inference="COORDINATES: protein motif:HMM:NF025636.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR-like C-terminal domain-containing protein"
                     /protein_id="HIR0912660.1"
                     /translation="TDVLNDSLEKNDTFDLSGQMGILLDLMQTYETYAAVLLSDHGDP
                     HFATRLKEVVWPLLNRYFVPSEGHDDYEMALLADFYLSGLLASVIRWLQNPKMTLEEF
                     LDFMVRTIFPIPDSPQSYNSCGKSVQTPADKSAGTPLP"
     gene            complement(337..2379)
                     /locus_tag="ACZO9R_06045"
     CDS             complement(337..2379)
                     /locus_tag="ACZO9R_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912661.1"
                     /translation="MRKLYVKLAAVNIRNNRQLYLPYLLAGVFSAAMFYLVMSIQDNP
                     GLEQMRGGAYVAVTLLLGVIIVGLFAAIFLFYTNSFIMKRRKRELSVYNILGMEKKHI
                     ARVLFLEMLFTAAVTIGGGLLFGIAFGKLMTMLLYRMTGFNQSVPFTVSLTGCHHTLV
                     LFGCIYAVTLFYNFMQIKLSNPMELLHSANAGEREPKTKVLLAVIGAAALGGGYYLAL
                     TVADAVSAITMFFVAVLLVILGTYCLFTAGSIAVLKLLRKNKNYYYKSRHFTTVSGMI
                     YRMKQNAVGLANICILSTMVLVTVSTTLCMYLGVEDALKRRFVHEVSVVSYYNAMPEH
                     PEEVDALAEASLKDSGRILTGHSAMLNMSLTAVRTDDGFSVTGIGAGTDYSISDVVLL
                     TILPKSDWENYTGEMVGELGSGEIALAASSACEKGMLALDNETYQVKQICAYPETDHD
                     YLDDMADDSVFMIVPDREALGQIFTELKQDWDEERVSLQIKYNMAFDIDGTAEEKVAA
                     ENVLHAAITAYNDGHPSDDAKDSYSRTYVECRAQNRDEYYSLNGGLLFIGLFLGAMFL
                     MVTVLIIFYKQISEGYEDKERYAIMEKVGMSNAEVKRSIRTQILTVFFLPIGAAVLHV
                     VMAFPMIKWILAAFSLNNTVLFALCVAATAVVFLLIYLLVFALTSRSYYKIVGNQV"
     gene            complement(2372..3145)
                     /locus_tag="ACZO9R_06050"
     CDS             complement(2372..3145)
                     /locus_tag="ACZO9R_06050"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912662.1"
                     /translation="MAILEVRNLKKIYTTRFGGNQVQALADLNFSAEKGEYVAIMGES
                     GSGKTTLLNILAALDKPTAGEVLLNNKNIVTLGEREISKFRRENLGFVFQDFNLLDHF
                     SLKDNILLPLVLADAPVQEMEERLWPIAEKLGIDVLLEKYPYEVSGGQKQRAAVARAL
                     ITRPQLILADEPTGALDSRATDGLLKLFGTINGEGQTILMVTHSTKAASHANRILFIK
                     DGEVFHQLYRGNMTNEELYVRISDTLTVLTTGKEGGYHA"
BASE COUNT          806 a          930 c          676 g          798 t
ORIGIN      
        1 ttacagatgt tctgaatgat tctctggaaa aaaatgatac ctttgacctt tccggtcaga
       61 tgggaattct tttagatctg atgcagacat atgaaaccta tgctgctgta ctcttaagcg
      121 atcatggcga cccgcatttt gcgacccgcc tgaaagaagt ggtatggccc ctcttaaacc
      181 gttattttgt tccctcggag ggacatgacg attacgagat ggcacttctt gccgattttt
      241 acctgtccgg ccttctcgcc agtgtcattc gctggctgca gaaccctaaa atgacactgg
      301 aagagttttt ggattttatg gtgagaacca ttttccctat acctgattcc ccacaatctt
      361 ataatagctg cgggaagtca gtgcaaacac cagcagataa atcagcagga acaccactgc
      421 cgtagccgcc acacacaggg caaacagcac ggtattgttg agactgaacg ccgccagaat
      481 ccatttgatc atcgggaacg ccatgacaac atgaagcact gctgccccga tcggcaggaa
      541 aaataccgtg aggatctgtg tgcggatact ccgctttact tcggcattgc tcatgccgac
      601 cttctccatg atggcatacc gttccttgtc ctcatagcct tccgaaatct gcttataaaa
      661 aatgatcagc accgtaacca tgaggaacat cgcgcccaga aacagaccga taaaaagcag
      721 accgccattt aagctgtagt attcatcccg gttctgcgcc cggcactcga catacgttct
      781 agaataagag tcctttgcat catccgacgg atgcccgtca ttgtatgcgg taatcgccgc
      841 atgaagtaca ttttccgctg ccactttttc ttctgccgtt ccgtctatgt caaacgccat
      901 attgtatttg atctgcagac ttacacgctc ctcgtcccag tcctgtttca actccgtaaa
      961 aatctgcccc agcgcctccc tatccggcac aatcataaat acactgtcgt ctgccatgtc
     1021 atcaagataa tcgtgatccg tctcaggata tgcgcagatc tgttttacct gatatgtctc
     1081 gttgtcgagc gccaacatcc ctttttcaca ggcagaggat gccgccagtg caatctctcc
     1141 gctgccaagt tccccgacca tttcaccggt ataattctcc caatcggatt ttggcagaat
     1201 cgtgagcagc accacatcac taatgctgta atctgtgcct gccccgatac cggtcacgga
     1261 aaatccatcg tccgtgcgca ccgcggtcag cgacatgttc aacatggcgc tgtgccccgt
     1321 cagtatccgt ccggaatcct tcagggatgc ttccgccagt gcatccactt cctccggatg
     1381 ttccggcatt gcattatagt aggacaccac agacacctca tgtacaaacc gccgctttag
     1441 ggcgtcttcc actcccagat acatacacag ggtggtagaa accgtcacca gaaccatcgt
     1501 cgataaaata cagatattgg caagccccac cgcattctgt ttcatccggt agatcatacc
     1561 ggaaaccgtc gtaaaatgtc ttgatttgta ataatagttt ttatttttcc gaagcagttt
     1621 gagtacggca atactgcccg cagtaaacag acaatacgtc cccagaatca caagcagcac
     1681 cgccacaaaa aacatggtaa tggcagagac tgcgtccgcc accgtaagcg ccagataata
     1741 gccgccgccg agtgccgccg ctccgatcac cgccagcagc actttcgtct tcggctcccg
     1801 ctctcccgca ttggcgctat gcagcagttc catcggattg gaaagcttta tctgcataaa
     1861 gttatagaaa agtgtgaccg cgtatataca gccgaaaagc accagcgtgt ggtggcatcc
     1921 cgtcagcgaa actgtaaacg gaacgctctg gttaaatccg gtcatgcggt ataaaagcat
     1981 cgtcatcagc ttgccgaatg caatgccgaa caacaggcct ccgccgatcg taaccgccgc
     2041 cgtaaacagc atttccagaa aaagcaccct ggcgatatgt ttcttctcca tcccaagtat
     2101 attgtatacg ctcaattccc gcttgcgccg cttcatgatg aagctgttgg tataaaacag
     2161 gaaaatcgcc gcgaacagtc cgacaatgat cactccgagc agcagcgtta ccgccacata
     2221 ggcgccgcct ctcatctgct ccagccccgg gttatcctga atggacatta ccagatagaa
     2281 catcgctgcc gagaacaccc ccgccagaag gtacggcagg taaagctgtc tgttattgcg
     2341 gatattgact gctgccagtt tgacatataa cttacgcatg atagcctccc tctttgccgg
     2401 tggtaagaac cgtcagcgta tccgaaatcc gcacatacag ctcctcattc gtcatattcc
     2461 cgcggtacag ctgatgaaat acttcgccgt ccttgataaa aagaatccgg ttggcatggc
     2521 tcgccgcttt cgtggaatgt gtcaccataa gaatcgtctg tccttccccg ttgatcgtgc
     2581 caaacagctt caacagtccg tcggtcgccc tggaatccag cgcccccgtc ggttcatctg
     2641 ccagaatcag ctgcggacgg gtgatcagtg cgcgggcaac cgccgccctc tgcttctgtc
     2701 cgccggaaac ctcataagga tatttctcaa gcagcacatc gatcccgagc ttttcggcaa
     2761 tcggccaaag ccgctcctcc atctcctgca ccggcgcatc tgccaggaca agcggcagaa
     2821 gaatgttatc ttttaatgaa aaatgatcca gcaaattaaa atcttgaaaa acaaagccca
     2881 ggttctccct tctgaatttg gaaatctccc tctcgccgag tgtcacaatg ttcttattat
     2941 tcaacagcac ttcccccgcg gtcggtttgt ctaacgccgc cagaatattg agcagcgtcg
     3001 tttttcctga accggactct cccataattg cgacatactc ccctttttcc gcggaaaaat
     3061 tcagatctgc aagcgcctgc acctgatttc ccccaaacct tgttgtatat atttttttta
     3121 gattacgaac ttccagaata gccatgtatc tctctccttt ccttgactgt acccagtata
     3181 acaaaacaga gaaatgcaag ccatttcttt
//
LOCUS       DBMDQJ010000246         3197 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_10934_length_3197_cov_1.186047, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000246 DBMDQJ010000000
VERSION     DBMDQJ010000246.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10934_length_3197_cov_1.186047"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(481..2184)
                     /locus_tag="ACZO9R_06055"
     CDS             complement(481..2184)
                     /locus_tag="ACZO9R_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912663.1"
                     /translation="MNIKKKILGITIAPVIILGLISMFLTTTMVRSAMLEEIEEALRG
                     TAAATLAAYDQNTGDYLQTSNGDIWKGSYNISKSESLVDRIKGNTGMDVTFFYGDTRI
                     MTSAVDAGGNRILNSKAGDRIVEKVLQGGESYFSHAVSIEGTLNYGYFIPVYQNGSTD
                     EIIGMIFVGTDKQEKDAVINKILGTISMAVCAVMILCIAIGMKLATSMSRNIRSSIEV
                     VGKVADGDLNVWVEDKLLGRHDEIGDLSRVTITLRDAMKATIQEISQNAKRLLDASEL
                     LGTAADQTNGTMDHVRMAVNQIVENATEQAQNSQNTSEHMRIMGENITETSAEVELLD
                     DNAASMQQSSEKAAETLNNLRNINVEVERIIGEVQEQTNRTNDSVQKIHEATAFITSI
                     AEETNLLSLNASIEAARAGESGRGFGVVADQIKKLSEQSNESSKEIEETAKTLSEDSA
                     KAVEIMQQMQEIIMNQSASMQDTQKVVEEVIAQIGSSMQSIRQIKESTGHLENSRNEV
                     LQAVSELSEIVQGNVDSTKKTYDETEEVVDTFKQVYVSAEQLREIADELVSSIDYFKM
                     Q"
     gene            complement(2239..>3197)
                     /locus_tag="ACZO9R_06060"
     CDS             complement(2239..>3197)
                     /locus_tag="ACZO9R_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR0912664.1"
                     /translation="THDLGIQGMQQNLGLSDDQIVRKINVSDSDEDATKQAIQECIDE
                     GCNIIFATSWGYMEATAEMAEKYPDVYFSHGTGYMSNGRNFNNYFGRIYQARYLSGIV
                     AGMNTTTNKIGYVAAMDNSNSEVTGGIDAFALGIYSVNPDAKVYVKVTNSWYDPEAEE
                     NAAKTLLDMDCDVIAQHCDTEYPQTLAQERGVYSIGYNSDMSKNAPEACLCSVIWNWS
                     AYYTAAVQSLIDGTWDGSNYYGGMNENLVALTNLADFCAEGTQEKVDEAKEQILSGQN
                     GVFDGVIETNTGETVGEAGKTLDDATITGGINWYFKTVNVVD"
BASE COUNT          685 a          946 c          668 g          898 t
ORIGIN      
        1 tacatttctt cctgtcggat ttttgcattg gattccgtgt ctcttccacg ttttgacaat
       61 ccatggaatg tgtccgacaa accgcagaca atgagcggat tccctagcgt ccctagcggc
      121 actgcttcac agacaatata aaaccgcgcg aaccgaactc gtcgtcatca gctattcaaa
      181 acagggcaga tgacgactca gacagcggct tcgcgcggtt atgttctatt cagcagtgcc
      241 gcaagggaca cacgagaatc ctaaaattgt ctgcaggtta cggacacacc cgtggagtgc
      301 tcaaaacttg gaaaaacatg gatcatcacg gatgcaaaaa ccgacggggt agaaatgccg
      361 cattttttga tccgaatttc cagatgcatt ccatctgaaa tcgaaggata tgatcttctc
      421 tttataatat tacttatcaa gtcaaaaagg ggctgcccca taagcgggca gcccctcgtt
      481 ttactgcatc ttaaagtaat caatactgct gaccagctca tctgcgatct cgcggagctg
      541 ctctgcactg acatacacct gcttgaaagt atccactacc tcttccgtct catcgtatgt
      601 cttcttcgtg ctgtccacat tgccctgcac gatttctgag agttcactca ccgcctgtag
      661 cacttcattt ctggaatttt ccagatgtcc tgtgctttct ttgatctgac ggatactctg
      721 catggagctg ccgatctgcg cgattacctc ttccactacc ttctgcgtat cctgcatgct
      781 cgcgctctgg ttcataatga tctcctgcat ctgctgcata atctcaaccg cctttgcaga
      841 atcctcactg agtgtcttcg cggtctcctc gatctccttg ctcgactcat tcgactgctc
      901 ggagagcttc ttgatctggt ctgccacaac accgaatcct ctgccgctct ctccggctct
      961 cgccgcctcg atcgaagcgt tcaggctgag aaggttcgtc tcctccgcaa tcgacgtgat
     1021 aaacgcggtt gcctcatgga tcttctgtac cgaatcgttg gtccggttcg tctgctcctg
     1081 cacttcaccg ataatgcgtt ccacctcgac attaatgttc cgcagattgt tgagcgtctc
     1141 tgccgccttc tcactggact gctgcatgga agctgcattg tcatccaaca gttccacctc
     1201 tgccgacgtc tcggtaatat tctcacccat aatacgcata tgttccgatg tattctggga
     1261 attctgtgcc tgttcagtgg cattctccac aatctggttg actgccatgc gcacatgatc
     1321 catggttccg tttgtctggt ctgccgctgt accgagcagt tcggacgcat cgagcaggcg
     1381 tttggcattt tgtgaaatct cctggatagt tgccttcatg gcatcccgca gcgtgatcgt
     1441 cacgcgggac agatcaccga tctcgtcgtg tctcccgagc agcttgtctt ctacccagac
     1501 attcagatcg ccgtccgcca cttttcctac cacctcaata ctgctgcgga tattgcggct
     1561 cattgaggtt gcaagcttca tgccgatcgc aatgcataag atcatcaccg cacacaccgc
     1621 catgctgatt gttcccagaa tcttgttgat cacggcatcc ttttcctgct tgtccgttcc
     1681 cacaaaaatc ataccgataa tctcatcggt cgatccgttc tgatataccg gtataaaata
     1741 tccatagttc aaagtgccct cgatcgacac cgcatggctg aaataggatt cgccgccctg
     1801 tagtaccttt tctacaatcc ggtcaccggc tttggaattt aagatgcggt tccctcctgc
     1861 atcgaccgcc gatgtcataa tgcgggtatc tccgtaaaaa aatgtgacgt ccattccggt
     1921 attgcctttg atacggtcta ccagactctc ggactttgaa atattataac tgcccttcca
     1981 gatatctcca ttgctggtct ggagataatc tcccgtattc tgatcgtagg ctgcgagcgt
     2041 ggctgccgcc gttccccgaa gtgcttcctc aatctcctcc agcatggcgc tccgtaccat
     2101 tgtggttgtc aaaaacatgg aaatcaaccc caatataata accggtgcaa ttgtaatccc
     2161 cagaatcttt ttcttaatgt tcatactaat ctccatttcg tctgtgcatc tgcatgtcgc
     2221 ttttcgtccg gctgctcctt aatccacaac attcaccgtc ttaaaatacc agttgatgcc
     2281 gccggtgatt gtcgcatcgt cgagcgtctt gcccgcttca ccgaccgtct ctccggtatt
     2341 tgtctcgatc acaccgtcaa atacaccgtt ctgtcccgaa aggatctgtt ccttcgcctc
     2401 gtctaccttc tcctgtgtgc cctccgcaca gaaatccgcc agattggtaa gagcgaccag
     2461 attctcgttc atgccgccgt aataattgct tccatcccag gttccgtcaa ttaagctctg
     2521 cactgccgcg gtatagtagg cactccagtt ccagataacg ctgcacaggc aggcctccgg
     2581 cgcattcttg ctcatgtcgg aattgtaccc gatgctgtat acgccacgct cctgggcaag
     2641 tgtctgcgga tactccgtat cacagtgctg ggcgatcaca tcacaatcca tatccagaag
     2701 tgtctttgcc gcattctcct ccgcctccgg atcataccag ctgttcgtca ccttgacgta
     2761 aaccttcgcg tccggattga ccgaatagat accgagcgcg aacgcatcga ttcctcccgt
     2821 cacctctgaa ttgctgttat ccatggcagc cacataaccg atcttgttcg tcgtggtatt
     2881 cattcccgcc acaataccgc ttaagtagcg cgcctgatag attctgccga aatagttatt
     2941 gaaattcctg ccgttgctca tataaccggt tccatgggaa aaataaacat cgggatattt
     3001 ctccgccatc tccgctgtcg cctccatgta accccagctg gtcgcaaaga taatgttgca
     3061 tccctcgtcg atacactcct gaatcgcctg ctttgtcgca tcctcgtcgg aatctgagac
     3121 attgattttc cgcacaatct gatcgtcgga aagtccgagg ttctgctgca ttccctgaat
     3181 tcccagatca tgcgtgt
//
LOCUS       DBMDQJ010000247         3178 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11006_length_3178_cov_1.861878, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000247 DBMDQJ010000000
VERSION     DBMDQJ010000247.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11006_length_3178_cov_1.861878"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="ACZO9R_06065"
     CDS             complement(<1..512)
                     /locus_tag="ACZO9R_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type ATPase actuator domain-containing
                     protein"
                     /protein_id="HIR0912665.1"
                     /translation="MDANITGLSAREVTERRERGEGGAAAERITKSRAQIVRENVFTL
                     FNLLNFIIAGLLFAVGAYTNMLFLAVIFLNIAVGIAQELKAKKLVDELSILNRPGVCV
                     RRGAKDIQIELDEVVKGDLMVLQSGNQICNDAVVVEGMLEVNESLLTGESDAVVKEAG
                     SELYSGSSVIS"
     gene            complement(1301..2635)
                     /gene="dnaB"
                     /locus_tag="ACZO9R_06070"
     CDS             complement(1301..2635)
                     /gene="dnaB"
                     /locus_tag="ACZO9R_06070"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR0912666.1"
                     /translation="MEEALVKRIMPNSLEAEQSVIGSMIMDKDAIVTAMEMLISEDFY
                     HTQYGILFDTMIELYNKGLPVDLVTLQNKLKEKDVPPEIASLEFVRDLVTAVPTSANV
                     KYYAQIVKDNSMRRKLIKLNEEIENECYVGKESVETVMDITEKKVFDLLSTRGGGGDY
                     VPIRQVVMNALEKIENAAKTSGTVTGIPTGFIDLDYRTAGLQPSDLILIAARPSMGKT
                     AFVLNIAQYVAFHENMCTAIFSLEMSKEQLVNRLFSLESRVDAQALRTGNLSDADWEK
                     LIEGAGTIGNSELIIDDTPGISISEMRSKCRKYKLEHDLKLVIIDYLQLMSGSGKSTD
                     SRQQEISDISRSLKALARELSVPVIALSQLSRAVEQRPDHRPMLSDLRESGAIEQDAD
                     VVMFIYRDDYYNHDSDMKGISEIIIAKQRNGPIGTVNLVWLPQYTKFANMEK"
     gene            complement(2651..3097)
                     /gene="rplI"
                     /locus_tag="ACZO9R_06075"
     CDS             complement(2651..3097)
                     /gene="rplI"
                     /locus_tag="ACZO9R_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR0912667.1"
                     /translation="MKVILLQDVKALGKKGDVVNVSDGYARNAIFPKKLGLEATPKNL
                     NDLKLQNQHADKVAQENYEAALALAEELKDKKITVKMKAGEGGRTFGSVSTKEIAAAA
                     KEQLGMELDKKKMQLPEPIRSFGMTEVAIRLHPKVTGTFTVHVVEE"
BASE COUNT          745 a          890 c          673 g          870 t
ORIGIN      
        1 gaaatgacag agcttccgga atataactca cttccagcct ccttgaccac tgcatcactc
       61 tctcctgtga gcagcgattc attcacttcc agcatgcctt ccacgaccac cgcatcgttg
      121 cagatctgat tgccgctctg caacaccatc aaatcaccct ttacaacctc atccagctcg
      181 atttgaatgt cctttgcccc gcggcgcaca catacgcccg gacggtttag aatcgagagt
      241 tcatccacca gctttttcgc ctttagctcc tgtgcaattc cgaccgcaat atttaaaaaa
      301 attaccgcca gaaacagcat atttgtgtaa gcacccacgg caaataaaag ccctgcaatg
      361 ataaagttca gcaaattaaa taatgtaaat acattttcac ggacaatctg cgccctgctt
      421 tttgtaatac gctctgcggc agcgccgcct tccccgcgct cgcggcgttc tgtcacttcc
      481 cgcgccgaca gtcctgttat attcgcatcc atgatctgtt cctcctgttt tgcttcggat
      541 cgtaatgtga gacattttcc aatggtaaca tgccaaaccg ccgctttcaa cgattgtcaa
      601 cgtttccata cgccgcagcc cgtttttgta ctctttcttg atacctgtgt aaggtactcc
      661 ccataagaat ggaactgccg caataagcat atcgcgcttt ggggtgataa gtaatattac
      721 aggaaaacca gagacaaaaa agttccagaa taaacagacg gcggcaaaat ctttgaagca
      781 aaattcgtgt atttcttcca taccgcggct ggcatcccat tccgtgtctt tgccacgttt
      841 ggacactcta ggagtgtgtc cgacaagccg cagacaatga gcggactttc tggtgtccct
      901 aatggcacct cttcacagac aatataaaac cgcgcgaacc gaaactcgtc ggcatctgct
      961 attgaaaata aggcagatgc cgactcagac atcggcttcg cgcggttatg ttttgttcga
     1021 cataaaggtg gcatttatgt cacctgttgt ctggtgccaa taagggacac acacaaaagt
     1081 cctaaaattg tctgccggct tgcggacaca atccgtagag tgctccaaac ttggaaagac
     1141 atggatcatc acggatgcca gctgcggcat ggttagaaat accagatttt gcggtcagat
     1201 ttgccgccgc atgtttatct ggaactttta agtatctatt tctaataata ttacttatca
     1261 cccaaaagat gtctatgctt attgctgcag ttccatccta ttatttttcc atattcgcaa
     1321 actttgtgta ctgcggcagc cataccagat tgacggttcc gatcggacca tttctctgct
     1381 tggctatgat gatctctgag attcccttca tatcggaatc atggttgtaa taatcatcgc
     1441 ggtagatgaa cattaccaca tcggcatcct gctcgatggc tccggactca cgcagatcgg
     1501 agagcatcgg gcggtgatcc ggccgctgct ccacggctcg cgagagctgg gagagtgcaa
     1561 ttaccggcac ggaaagctcc ctcgcgagtg ccttgaggga tcgggaaata tccgaaatct
     1621 cctgctgacg ggagtctgtt gacttcccgg aaccggacat gagctgcagg tagtcgatga
     1681 tgaccagctt cagatcatgc tctaatttat attttctgca cttgcttcgc atctcggaga
     1741 tcgagatacc cggcgtatca tcgatgatca gctcggaatt accgatggtt ccggctccct
     1801 cgatcagctt ctcccagtct gcatccgaga gattaccagt acggagcgcc tgcgcatcca
     1861 ctctggactc cagagaaaac agacggttga caagctgctc cttggacatc tcgagcgaaa
     1921 agatggcggt acacatattt tcgtgaaatg ccacatactg tgcaatgttt agtacaaacg
     1981 ccgtcttacc catagacgga cgcgctgcaa tcaggatcag atcggacggc tgcagaccag
     2041 cggtgcgata atccagatca ataaatccgg tcggtattcc ggtaaccgtt cctgaagtct
     2101 ttgccgcgtt ctcgatcttc tcgagggcat tcataacgac ctgccggatc ggcacgtagt
     2161 cgccgcctcc gccgcgggtg gagagaaggt cgaatacctt cttctcggtg atatccatga
     2221 ctgtctcgac gctttccttg ccgacataac actcattctc gatctcttca ttcagcttga
     2281 tgagctttct gcgcatcgag ttgtccttga cgatctgtgc ataatacttc acattcgccg
     2341 aagtcggtac tgccgtcacc agatcccgca caaactccag actcgcaatc tccggcggaa
     2401 catctttttc cttaagcttg ttctgcagtg tcaccagatc cacagggaga cctttgttgt
     2461 acagttcaat catcgtatca aacaaaatcc cgtactgcgt gtggtagaag tcctcgctga
     2521 tgagcatctc catcgctgtc acgatggcat ccttatccat aatcatggaa ccgatcacgg
     2581 actgctctgc ctcaagactg ttgggcataa tccgctttac cagtgcctct tccatgaaat
     2641 aaacctcctg ctactcttct acaacatgaa cggtaaatgt tcccgttacc ttcggatgaa
     2701 gcctgatggc aacctctgtc atgccgaagc ttctgatcgg ctccggaagc tgcatctttt
     2761 tcttgtcgag ctccatgccg agctgctcct ttgccgctgc cgcaatctcc ttcgtggaaa
     2821 cagatccgaa ggtacgtcca ccctcgccgg ctttcatctt gaccgtaatt ttcttatcct
     2881 ttaattcctc cgcaagcgca agcgccgcct cgtaattctc ctgtgcaacc ttgtctgcgt
     2941 gctggttctg caatttcaga tcattgaggt tcttcggtgt cgcctcaagt cccagctttt
     3001 tcgggaaaat cgcatttctc gcatacccgt cactgacgtt caccacgtcg cctttttttc
     3061 ctaatgcctt tacatcctgc aataaaatta ctttcatatg atcctcattt ccgcgcattc
     3121 tgctgcgctt atcttgtatt tcccaaatcg tattattcct cgatgtcgcc ttctttta
//
LOCUS       DBMDQJ010000248         3170 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11051_length_3170_cov_1.397849, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000248 DBMDQJ010000000
VERSION     DBMDQJ010000248.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11051_length_3170_cov_1.397849"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            324..1028
                     /locus_tag="ACZO9R_06080"
     CDS             324..1028
                     /locus_tag="ACZO9R_06080"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpnC/YadD family protein"
                     /protein_id="HIR0912668.1"
                     /translation="MEKLATTVSEPHAKREYKDTVFRMLFSDKEKLLSLYNAMNDRHY
                     ENAEDLKIVTLKNAIYMGMKNDLAFMVDTQICLYEHQSTKNPNMPLRDMFYISLEYQA
                     YVNNKSLYSSTLQKLPTPKFVIFYNGTEDLEECTELRLSDAYENLEDFLRENRAEVEM
                     VSILEYDKDFEEKKLREAEYEAGREEGARFGLAEGIVETGCAYNVPEPDILARLQEKL
                     DIPLRKAQEYFAMYSK"
     gene            1177..>2529
                     /locus_tag="ACZO9R_06085"
     CDS             1177..>2529
                     /locus_tag="ACZO9R_06085"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR0912669.1"
                     /translation="MKNKKRILTGLLAAALVVPLFAGSAQTAAAAEAAGKTLDVVFTH
                     DTHSHLNSFSTVIDGESTEVGGFARIKTVIDEQKAENPDTLVVDGGDFSMGTLVQTVY
                     EDEAAELRMLGAIGCEVTTFGNHEFDYRSSGLAQMLKSAAESGDVLPELVVCNVDWDA
                     MEAAGLNDGQQQIQSGFEEYGVKDYVVLQKGDVNVAVTGVFGVDALACAPTCELLFRD
                     PVEAVRETVAEIKANEDVDMIVCVSHSGTWEDESKSEDEILAKSVPDLDLIISGHTHT
                     ELAEPIVHGDTYIVSTGEYGRKIGELSMTQKSDGRWEITDYKLVPILSTITADGATQE
                     KIDGFMEAVDTGYLADFGYTKDEVLATNNVQFSTLHDLEYEHTEHNLGDLMSDAYVYA
                     VENSDDFDGVPVDVAVVPSGTVRDTYTAGDITVEQVFNSFSLGIGPDGVPGYPLISVY
                     L"
     assembly_gap    2530..2539
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2540..3165
                     /locus_tag="ACZO9R_06090"
     CDS             <2540..3165
                     /locus_tag="ACZO9R_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016288025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR0912670.1"
                     /translation="TGKELRTAAEIDASVSDYMTTARLYMSGLHFTYNPNRMILNKVT
                     DVYLTDENGSRVELEDDKLYRVVADLYSGQMLSAVTDMSHGLLSLVPKYADGTPIEDF
                     EDVIVMEGDRELKAWDSIARYMQSFEDTDGDGIANVPSYYSQMHDRKVVDESRSLGDL
                     VKSPNKYAAMIIAVILIVIILVVLLIVLVVKLIKRIRRRKADTIHKR"
BASE COUNT          890 a          624 c          919 g          727 t
ORIGIN      
        1 gcattaaaac aaaatataag agtataggga agaggggtaa cacactgtat agcgtgttac
       61 ccctcttcct tataagaaac accatataca gaggagagac agggaaaagg cagagagaaa
      121 atacataaaa agagaaaaag atgcttgtag gaacatcaca tagatggtaa aataatcaca
      181 tcaacaaaag catatttcta acgagtccag gtgcatgcac cgtctatatt ctaatcgcat
      241 ttcaaaggtc tatgctttta aactcatttt aaggaaaaga gcagggatca tgaaaagcga
      301 tccctgacaa gggggaactg aatatggaaa aattggcaac gacagtcagc gaaccgcatg
      361 caaagcgtga gtacaaggat acagtattcc ggatgttgtt ttctgacaag gaaaaactgc
      421 tgtcgctcta caacgccatg aatgaccggc actacgaaaa cgcagaagac ttaaaaattg
      481 ttaccttgaa gaacgcaatt tacatgggaa tgaaaaacga tcttgcgttt atggtagata
      541 cccagatctg cctttatgag catcagtcca cgaaaaatcc gaacatgccg ctgcgtgaca
      601 tgttctatat ttcgctggaa tatcaggcat atgtaaataa taaatcgctg tactcctcga
      661 cattgcagaa actgcccact ccgaagtttg tcatatttta taatggaaca gaagatctgg
      721 aagagtgtac agaactccgc ctgtccgatg catatgagaa tctggaagac tttttgaggg
      781 aaaaccgtgc ggaggtggaa atggtgagta tactggagta cgacaaggac tttgaagaaa
      841 aaaagctgcg ggaagcggaa tacgaagccg gacgcgagga aggagcgcgg tttggacttg
      901 cagagggaat cgttgaaact ggctgtgcat acaatgttcc ggaaccggac attctggcac
      961 gtttgcagga aaagctggat attccattga ggaaggcgca ggagtatttt gcgatgtatt
     1021 ctaagtgatc atagaagtaa gaggaacagg gctgtggcat ttgcaatagc cctgttttca
     1081 tggtaaaata aacctatctt aagagctgat tgggatctaa gggggtccga tgagtgtaaa
     1141 tggggagtat atttcataat taaggagggt ttttgaatga aaaacaaaaa aagaattctg
     1201 acagggctgc ttgcggcagc cttggttgtg ccgctgtttg ccggcagcgc acagacagct
     1261 gcggctgcgg aggcggccgg gaagacactg gatgtggtgt tcacacacga tacccactcc
     1321 catctgaaca gtttttccac agtgattgac ggagaatcca cagaggtggg cggttttgca
     1381 aggatcaaga cagtgatcga tgagcagaaa gcggagaatc cggacacgct tgtggtcgat
     1441 ggcggtgatt tttccatggg tacgctggtg cagaccgtct atgaggatga ggcggcggag
     1501 cttcggatgc tgggcgcaat cggctgtgaa gtgacgacgt ttggtaacca cgagtttgat
     1561 taccgttcca gcggactggc acagatgcta aagagtgcag ccgaaagcgg cgacgtactg
     1621 ccggagcttg tggtgtgcaa tgtagactgg gacgcgatgg aggctgcggg attaaatgac
     1681 ggacagcagc agatccagag cggatttgag gagtacggcg tgaaggatta tgtcgtgctg
     1741 caaaagggag acgtcaatgt cgctgtgaca ggtgtgttcg gtgtggacgc acttgcctgc
     1801 gcgccgacct gtgaactgtt attccgcgat ccggtggaag cggtcaggga gacggtggca
     1861 gagatcaagg caaatgagga tgtggacatg atcgtctgcg tatcacacag cggtacgtgg
     1921 gaggatgaga gcaaatccga ggatgagatt cttgccaaga gtgtgccgga tcttgacctg
     1981 attatcagcg gtcataccca cacagagctc gcggagccga tcgtgcacgg cgacacgtat
     2041 attgtatcca cgggagagta cggcagaaag atcggagaac tttccatgac ccagaagagc
     2101 gatggcagat gggagataac ggattataag ctggttccga ttctttcgac catcactgcg
     2161 gacggagcga cccaggagaa gattgatggc ttcatggaag cggtggatac cggttatctc
     2221 gcggatttcg gttacaccaa ggatgaggtg cttgcaacca ataatgtgca gttttccacg
     2281 ctgcatgatc tcgagtatga gcacacggaa cacaatctgg gtgacctgat gtcggacgcc
     2341 tatgtgtatg ccgtggagaa ctccgatgat tttgacggag taccggtgga cgttgccgtg
     2401 gttccgagcg gtacggtgcg cgatacctac acggccgggg acattacggt ggagcaggtg
     2461 tttaattctt tttcccttgg cattggtccg gacggcgtac cgggataccc gctgatcagt
     2521 gtctatctgn nnnnnnnnnt gacagggaag gaacttcgca cagccgcgga gattgacgcg
     2581 tccgtttctg attacatgac gacagccaga ctttacatga gcggacttca ttttacatac
     2641 aatccgaacc gcatgatttt aaataaggtg acggatgttt atctgaccga tgagaacgga
     2701 agccgcgtgg agcttgagga cgataagctg taccgcgttg tggcagatct ctacagcgga
     2761 cagatgttaa gtgcggtgac ggatatgtcg cacggactgt tgtcgcttgt gccaaagtat
     2821 gcggacggaa cgccgatcga ggattttgag gacgtcatcg tgatggaggg ggaccgcgag
     2881 ttaaaagcgt gggattcgat tgccagatat atgcagtcct ttgaggatac agacggcgac
     2941 ggtattgcca atgtgccgtc ttattacagc cagatgcacg accgcaaggt ggtggatgag
     3001 agccgctcgc tcggagatct tgtaaagagc ccgaataaat atgcggctat gattatcgcc
     3061 gtgattctga ttgtgataat tctggttgta ttactgattg tgttagtggt aaaattgata
     3121 aaacgtatac ggagacggaa agcagataca atacataaaa gataattgaa
//
LOCUS       DBMDQJ010000249         3168 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11062_length_3168_cov_1.640365, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000249 DBMDQJ010000000
VERSION     DBMDQJ010000249.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11062_length_3168_cov_1.640365"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1972)
                     /locus_tag="ACZO9R_06095"
     CDS             complement(<1..>1972)
                     /locus_tag="ACZO9R_06095"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR0912671.1"
                     /translation="VNWCPVCKTSISDAEVEHEEQDGFFWHINYPVVGEEGRFVEIAT
                     TRPETLFGDTAVAVNPDDERYKDIVGKMLKLPMTDREIPVIADPYVDKEFGTGCVKIT
                     PAHDPNDFEVGKRHNLEEITVINDDATMNHFAGKYEGMDRYECRKALVDDLKEQGLLV
                     KVEPHSHNVGTHDRCGTTVEPMVKQQWFVKMDELIKPAVEAVKNGDIQLLPKRMEKTY
                     FNWTDNIRDWCISRQLWWGHRIPAYYCPDCGEMVVAKSAPQKCPKCGCGHMEQDPDTL
                     DTWFSSALWPFSTLGWPKKTEDLDYFFPTDVLVTGYDIIFFWVIRMIFSGYEQMGKAP
                     FHTVLFHGLVRDSQGRKMSKSLGNGIDPLEVIDKYGADALRLTLITGNAPGNDMRFYW
                     ERVESSRNFANKVWNASRFIMMNLEGAAITEPSADELQPADKWILSKVNTLARDVTEN
                     MDKYEMGIAVQKVYDFIWDEFCDWYIEITKTRTYNKEQDPVSANAAFWTLKTVLTEAL
                     KLLHPFMPFITEEIFCTLHPKEETIMLAKWPEYRANWNFPAEEEMLEHCKDLVKGVRN
                     VRTEMDVPPSRKARIFIVADDAALRETFEKTREAYQNLAGASDVSVQADKNGIEDDAV
                     SVVIPGATLYLPLEDLVDFEKEKERLLKEKERL"
     assembly_gap    1973..1982
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1983..2489)
                     /locus_tag="ACZO9R_06100"
     CDS             complement(<1983..2489)
                     /locus_tag="ACZO9R_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR0912672.1"
                     /translation="MCQNCSKELAKTYDPKGIEGRLYKKWEDNGYFHAEVDRSKKPFT
                     IVMPPPNITGQLHMGHALDNTMQDILIRYKRMQGYNALWQPGTDHASIATEVKVIENL
                     KKQGIDKHDLGREGFLEKCYEWKDEYGSRIINQLKKMGSSADWQRERFTMDKGCSDAV
                     LEVFVKLYE"
     gene            complement(2857..>3168)
                     /locus_tag="ACZO9R_06105"
     CDS             complement(2857..>3168)
                     /locus_tag="ACZO9R_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912673.1"
                     /translation="FCLLVRIGTGSYEVSESMRRECEKRQQALGCHLADRIVESLMCQ
                     GVSEGTIGETNPYLQKLAFVAQMTAFAGYLVDLGKGEKMAVSREEAKRFVYEALVKSL
                     N"
BASE COUNT          646 a          951 c          696 g          865 t
ORIGIN      
        1 cagacgctct ttttctttta agaggcgttc cttctccttc tcaaaatcga ccagatcctc
       61 aagcggcaga tacagtgttg ctcccggaat tacgacagat accgcatcgt cctcgattcc
      121 gttcttgtct gcctgtacac tcacatcgct tgcgcctgcc agattctgat atgcctctct
      181 tgtcttctca aatgtctcac gcagtgcggc atcgtctgcc acaatgaaaa tccttgcttt
      241 tcttgaaggc ggcacatcca tctccgtacg cacgttgcgg acacctttta ccagatcctt
      301 gcagtgctct aacatctctt cctctgccgg gaaattccag tttgcgcggt actccggcca
      361 ttttgccagc atgatcgtct cctctttggg atgcagtgtg cagaaaatct cctccgtgat
      421 aaacggcata aacggatgca gcagctttaa cgcctctgtg agcaccgtct ttaatgtcca
      481 gaacgccgca tttgctgaaa ccggatcctg ctctttgttg taggtgcgcg tcttggtaat
      541 ctcaatgtac cagtcacaga actcatccca gatgaagtcg taaaccttct gcactgcgat
      601 tcccatctcg tacttgtcca tgttctcggt cacgtccctg gcaagtgtat tcacctttga
      661 gagaatccac ttgtccgccg gctgtaactc atccgcggac ggctcggtaa tcgcagcccc
      721 ctccagattc atcatgataa aacgggatgc attccagact ttgttggcaa agtttctgga
      781 tgactcgact ctctcccagt agaaacgcat gtcgttgccc ggtgcatttc cggtaattaa
      841 cgtcagacgg agcgcatctg cgccgtactt gtcgatgacc tcaagcggat caataccgtt
      901 gccgagcgat ttgctcatct tgcgtccctg ggaatcgcgc accagtccgt ggaacaatac
      961 cgtgtggaac ggtgcttttc ccatctgctc gtaacccgag aagatcatac ggatgaccca
     1021 gaagaaaata atgtcgtaac ctgttaccag cacatcggtc gggaagaagt agtccagatc
     1081 ctcggtcttc ttgggccagc ccagtgtcga gaacggccac agtgcggagg agaaccaggt
     1141 gtcgagcgta tccggatcct gctccatgtg gccgcatccg cacttcgggc acttctgggg
     1201 cgcactcttt gccacaacca tctcaccaca gtccggacag tagtaagccg gaatgcggtg
     1261 tccccaccag agctgtctgg agatgcacca gtcgcggatg ttgtccgtcc agttaaaata
     1321 ggtcttctcc atgcgcttcg gcagcagctg gatatctccg ttcttcactg cctccactgc
     1381 cggtttgatc agctcatcca tctttacaaa ccactgctgc ttcaccatcg gctctaccgt
     1441 cgtaccgcag cggtcgtgtg ttcctacatt atgagagtgc ggctccacct ttaccagaag
     1501 tccctgctcc ttcaagtcgt ccaccagtgc ctttctgcat tcgtagcggt ccatcccctc
     1561 gtattttcct gcgaaatggt tcatcgtcgc atcgtcgtta atgacggtga tctcctctaa
     1621 gttgtggcgc tttccaacct caaagtcgtt cgggtcatgt gccggtgtga tcttcacgca
     1681 gccggttccg aactccttgt cgacatatgg gtctgcgatg accggaatct cacggtctgt
     1741 catcggaagc tttaacattt ttccaacgat gtccttgtag cgctcatcgt ccggattgac
     1801 agcgactgcg gtatcaccga acagcgtctc cggtctggtt gttgcgatct caacgaatct
     1861 gccttcctcg ccgactaccg gatagttgat gtgccagaaa aatccgtcct gctcctcatg
     1921 ctctacctcg gcatcggaaa tggaggtctt gcagaccggg caccagttga cannnnnnnn
     1981 nnttcatata acttcacaaa tacctcaagc actgcgtcag agcagccctt gtccatggta
     2041 aaacgctctc tctgccagtc tgcggaggaa cccatcttct ttaactggtt gatgatgcgg
     2101 ctgccgtact cgtccttcca ctcatagcat ttctcgagaa acccttcgcg tcccagatcg
     2161 tgcttgtcaa ttccctgctt tttcaggttc tcaataacct ttacctcagt cgcaatggag
     2221 gcgtggtcgg ttcccggctg ccacaatgca ttgtacccct gcattctctt gtaacggatc
     2281 aaaatatcct gcatggtgtt gtcgagcgca tgtcccatat gaagctgtcc ggtaatattt
     2341 ggcggaggca tcacgatggt aaacggtttc ttgctacggt caacttccgc atggaaatag
     2401 ccgttgtcct cccatttttt gtaaagtctt ccctcgatcc ccttcggatc atatgtcttg
     2461 gcaagttctt tgctgcaatt ctggcacatt gtcctctctc cttttctttc ctaattacat
     2521 aaaaacgccc tttgtatccg taaggataca aagggcgact tatcgcggta ccacctttgt
     2581 tcaccagctg tatacagccg gtcttcgctc cggtcagatg ctctctaacc agcggttcgt
     2641 taacgggaac cgggcgtgaa cacttgctgc cgtacatctt ctgtaccgct ttctgggtgt
     2701 tccggttcaa aagctacctt ccacgcatct ccttccaaga aaccttgcag ccgatgggtc
     2761 tctctctctg cggcgagtct gcatgtactc ctctttctct ctacctttta catactgtta
     2821 atgatacgtg cagcgtcccg ttttgtcaag aaatttttag ttcagactct tcaccagcgc
     2881 ctcatagaca aagcgcttcg cctcttccct gcttaccgcc atcttctctc cttttcccag
     2941 atcgaccagg tatcctgcaa acgctgtcat ctgcgccacg aacgccagct tctgcaagta
     3001 cggattcgtc tccccgatcg tgccttccga cacgccctgg cacatcaggg actccacaat
     3061 cctatccgcc agatggcagc ccagagcctg ctgtctcttt tcacattccc tgcgcatgga
     3121 ctcagagacc tcatacgaac cagttccgat ccgtaccaga agacagaa
//
LOCUS       DBMDQJ010000250         3166 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11074_length_3166_cov_1.747145, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000250 DBMDQJ010000000
VERSION     DBMDQJ010000250.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11074_length_3166_cov_1.747145"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2112
                     /locus_tag="ACZO9R_06110"
     CDS             <1..2112
                     /locus_tag="ACZO9R_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR0912674.1"
                     /translation="KFGKAVVKLRIPILILSIFLLIPSAIGYFNTRVNYDILTYLPEE
                     IETMKGQDLLLDEFGTGAFSFCVVEGMDAKDISAMREQMEEVPHVKSVIWYDSLADIS
                     IPMEMLPDEIYDFFNNDEKDSTLLAVLYDTSMSADETMDAIEELRGVVKDQCYISGMS
                     AVVTDIKNLSNKETPVYVLIAVVLAVIVLSLTMDSALAPIFFLLSIGMAIVYNLGTNV
                     FKGEISYVTQALAAVLQLGVTMDYSIFLWHSYEEQQERFQGDKKRAMAHAISNTITSV
                     VGSSITTVAGFVALCFMSFTLGMDLGIVMAKGVVLGVICCVTVLPSLLLVFDKAIEKT
                     KHKVIIPDLGRIADWIVRHYGVFLIVFLVVLGPAVYGYTHTDVYYDLAGTLPKSLSSI
                     TANDKLNEDFEMGATHMIIADSSLSAKDASAMLDEISKVDGVKMALGFDSLVGPGIPR
                     EFIPDDVKDVLINGDYQMMVVGSEYAVASDEVNAQCEEINNIIKKYDSSAMLIGEAPC
                     TKDLIEITDEDFKTVSIVSIGAIFVIIALVFKSVSLPVILVAVIEFAIFINMGIPAYT
                     HTTLPFIASIVIGTIQLGATVDYAILMTTKYKKLRYNGADKKEAIATALRSSIQSIIV
                     SALSFFAATFGVGMYSNIDMISSLCILMARGAIISMFVVIFILPSMFMIFDKLICATS
                     MGFRKKKGATVNVSENNVSVQ"
     gene            2134..>3166
                     /locus_tag="ACZO9R_06115"
     CDS             2134..>3166
                     /locus_tag="ACZO9R_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912675.1"
                     /translation="MKLPELKSKFKNRYAIRIIAGVLVVTLVATGSSVYSVNAAKAGS
                     AATETVEETEVSEEAEDTEDAESSLKDLLDNGSKVSEKEIGKEETVYVIADNTGKEQK
                     IIVSDHLINNDDKDTLEDASTLKDIENVKGDETFTQSGNKVTWQADGNDIFYQGTSES
                     ELPVTQKLTYYLDGKEVTPEELAGKSGEVTIRFDYTNNQKVTAKIDGKDEDIYVPFMA
                     VSGMILGDEFSDIEVENGKVISDGSNNVVVGYALPGLKESLNVKDDDFDGDVTIPDYV
                     EVKAKVENFKLDTTMTVVMNATNFISADSDNDTSKLDEVFDTLTDAMDQLTDGSAELA
                     DGVDTLKSKM"
BASE COUNT          850 a          643 c          896 g          777 t
ORIGIN      
        1 aaatttggaa aagcggtcgt caagctgcga ataccgattt tgattctcag tattttctta
       61 ttgataccgt cggcaatcgg atattttaac acgagagtga attatgatat cctgacatat
      121 ctgccggaag agattgagac gatgaagggg caggatctcc tgcttgacga gtttggaacg
      181 ggagcattct cgttttgtgt agtggagggt atggacgcga aggacatatc tgcaatgcgg
      241 gagcagatgg aggaagttcc tcacgtaaaa tcagtgatct ggtacgattc actggcggac
      301 atcagcattc cgatggagat gctgccggat gagatctatg atttctttaa taatgatgag
      361 aaggacagca ccttgctcgc agtgttatac gatacatcga tgtccgcgga cgagacgatg
      421 gatgcgattg aggaactgcg cggtgtggta aaagatcagt gctatatcag cggtatgtcg
      481 gcagtggtta cagatatcaa gaatctgtcg aacaaggaga ccccggtcta tgtgctgatt
      541 gcggttgtac tggcagtcat tgtcctgtca cttacgatgg attcggctct ggcgccgatt
      601 ttctttttgc tcagtatcgg aatggcgatt gtatacaatc tgggtaccaa tgttttcaag
      661 ggagagattt cctatgtgac acaggcgctc gcggcggtct tacagctggg tgtgacgatg
      721 gattattcca ttttcctctg gcacagttac gaagagcagc aggagcgttt tcagggagat
      781 aaaaaacgtg cgatggcgca tgcgatttct aatacgatca catcggtggt cggaagctcc
      841 atcacgacgg tggctggttt cgtggcactg tgtttcatga gctttacact tggtatggat
      901 cttggaattg tcatggcaaa gggtgttgtt ctcggcgtta tctgctgcgt tacggtgctg
      961 ccgtcgctgc tgcttgtatt tgacaaggcg atcgagaaga cgaagcacaa ggttatcatc
     1021 ccggatctgg gacggattgc agactggatc gtgcgccatt acggcgtatt cctgatcgta
     1081 tttctggtgg tgttgggccc ggcagtctac ggctacacac acacggatgt gtattacgat
     1141 cttgcgggaa cactgccgaa gagcttaagc agtattactg cgaacgataa gttaaacgaa
     1201 gactttgaga tgggtgcgac gcacatgatc attgcggaca gcagtctctc cgcaaaagat
     1261 gccagcgcaa tgttggatga gatcagcaag gtggatggcg tgaagatggc gcttggcttt
     1321 gactcgctgg taggaccagg catcccgagg gagtttatcc cggatgacgt aaaagacgtc
     1381 ttgatcaacg gcgattatca gatgatggtg gtcggctcgg aatatgcggt tgcttctgat
     1441 gaagtgaacg cgcagtgcga ggagatcaac aatatcatca aaaaatatga ttcctctgcc
     1501 atgttaatcg gtgaggcacc gtgtacgaag gatctgattg agatcacgga tgaggacttt
     1561 aagacagtca gcatcgtgtc gatcggtgca attttcgtga ttatcgcact ggtatttaag
     1621 tcggtatcac tgccggtcat tctggtggcg gtcattgagt tcgccatttt tatcaacatg
     1681 ggtattccgg catatacgca cacgacgctg ccgtttatcg cttctatcgt cattggtacg
     1741 attcagctcg gcgcgacggt cgattatgcg attctcatga caacaaagta caagaagctt
     1801 cgctacaacg gagcggacaa gaaggaagcg attgcgacgg cacttcgcag ttccatccag
     1861 tcgatcattg tcagtgcgct cagcttcttt gcagcgacct tcggtgtcgg catgtattcc
     1921 aatattgata tgatcagttc cctgtgtatt ctgatggcac gcggagcaat catcagtatg
     1981 tttgtcgtta ttttcattct gccgtccatg tttatgattt ttgataaact gatctgtgcg
     2041 acgagcatgg gatttcgcaa gaaaaagggc gcgacggtga atgtgtcgga aaacaatgta
     2101 tcggtacagt aacgggaaag gaaggtaaaa accatgaaat taccagagtt aaaatcaaaa
     2161 ttcaaaaata gatatgcaat tcgtattatt gccggagtcc tggtcgttac gcttgttgca
     2221 acaggctcct ccgtctacag tgtgaatgcg gcaaaggcag gcagcgctgc cacggagacc
     2281 gtggaggaga cggaagtttc tgaggaggcg gaagatacgg aagatgccga gagcagctta
     2341 aaggatctgc tggataacgg aagcaaagta agcgagaaag agatcggaaa agaagagacg
     2401 gtatatgtca tcgctgataa taccggcaag gagcagaaaa tcattgtctc cgatcatctg
     2461 atcaacaacg atgacaagga tacgcttgaa gatgcatcca cattaaaaga tattgaaaat
     2521 gtaaaaggcg atgagacctt tacacaaagc ggcaataagg tgacatggca ggcagacggc
     2581 aatgacattt tctaccaggg aacgtcagag agcgagctgc cggtgacaca gaagctgacc
     2641 tactatctgg acggcaagga agtgacgccg gaggagcttg ccggaaaatc cggtgaggtt
     2701 acgatccggt tcgactacac gaacaaccag aaagtgacag cgaagattga cggaaaagac
     2761 gaagacatct atgtaccgtt tatggcagtc agcggcatga tcctcggaga tgaattctcg
     2821 gatatcgagg tggaaaacgg taaggtgatc tccgacggaa gcaacaatgt cgtagttggc
     2881 tatgcacttc cggggctgaa ggaaagcctg aacgtaaagg acgacgattt cgacggggat
     2941 gtcaccattc cggattacgt ggaggtaaag gcaaaggttg agaacttcaa gcttgatacc
     3001 acgatgacgg tggttatgaa tgctacaaac ttcatcagtg ccgacagtga caatgacacc
     3061 tcgaagttgg atgaggtatt tgacacactc acagacgcga tggatcagct gaccgacggt
     3121 tctgcagaac tggcagacgg cgtagacacc ttgaagtcca agatgg
//
LOCUS       DBMDQJ010000251         3158 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11119_length_3158_cov_1.593392, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000251 DBMDQJ010000000
VERSION     DBMDQJ010000251.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11119_length_3158_cov_1.593392"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /gene="polA"
                     /locus_tag="ACZO9R_06120"
     CDS             <1..1630
                     /gene="polA"
                     /locus_tag="ACZO9R_06120"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR0912676.1"
                     /translation="EEICYVEASEALPCRKIRELFAGSAAASYATLDLKPQLKELGML
                     EKKCLGTISEENLFDNTIAAYLLNPIKNEYPYEDIAKDYAGLMILSRKDLIEKLTYEK
                     AAEKKPEEFLKCVCYMAYVAYKTREPLEKELADTGMEALFREIEMPLVFTLADMEKEG
                     IIASGEALKEYGDKLAVRIDELERKIYEEAGEEFNINSPKQLGVILFEKLSLPNGKKT
                     KTGYSTAADVLDRLAPDYPIVADILEYRQLTKLKSTYADGLVNYIAEDGRIHTSFNQT
                     ITATGRLSSTEPNLQNIPMRIELGRLIRKAFLPKTGFVFVDADYSQIELRVLAHLSGD
                     EKLIEAYRNAQDIHRTTASQVFHIPFDEVTDLQRRNAKAVNFGIVYGISSFGLSQDLS
                     ITKKEAAEYIERYFETYPKIKGYLDGLVAEAKEKGYVTTMFGRRRPIPELSSSNFMQR
                     SFGERIAMNSPIQGTAADIIKIAMNRVHDRLLAEGLKSRLILTVHDELLVETAAEEEQ
                     EVRKILAEEMHGAAQLSVMLEIDVHAGSDWYQAK"
     gene            1646..2245
                     /gene="coaE"
                     /locus_tag="ACZO9R_06125"
     CDS             1646..2245
                     /gene="coaE"
                     /locus_tag="ACZO9R_06125"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080137.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR0912677.1"
                     /translation="MRFIGITGGVGAGKSEILGYLAKKPDTRVMLADEIAHELMSPGA
                     ECYDRICETFGAEDIFLPQGGLDRGKLAAVIFSDEAKRRQMNAIVHPAVRVYVEKEAA
                     REKQGGKRKLLVLEAALLIEEHYDEICDELWYIYTREDIRRERLMASRGYSTEKVDRI
                     FASQLTEEVYREHCRVVIDNNGSVAEAFAQIDRALSEKK"
     gene            2265..>3158
                     /locus_tag="ACZO9R_06130"
     CDS             2265..>3158
                     /locus_tag="ACZO9R_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR0912678.1"
                     /translation="MSDTSVFDQPLVFGLDIGTRNVVGTVGYKTENEFVVVAQYMLEH
                     ETRAMLDGQIHDIGRVGHTIAKVKEELESQIGQELTEVCIAAAGRVLKTVTTNISYDF
                     PEETVVTGEDIHTLDLLGIEKAQSILKEMNDTRYKFYCVGYSVMKYFLNDEPFSSLES
                     HKAERISEDIIVTFLPEDVVDGLYAAVGMAGLTVANMTLEPIAAINVAIPENYRLLNI
                     ALVDIGAGTSDISVTRDGSIIAYGMIPLAGDEITELIVQSYLVDFNTAEQIKLSSGME
                     DQVTYKDIMMIEHTIPSKDVWK"
BASE COUNT          825 a          655 c          990 g          688 t
ORIGIN      
        1 ggaggaaatc tgttacgtgg aggcatcgga agcacttccc tgccgtaaga tccgtgaatt
       61 gtttgcgggg agcgcagccg cttcctatgc gacacttgac ttaaagccgc agttaaagga
      121 actggggatg cttgagaaaa aatgtcttgg aacgatctcg gaggagaacc tgttcgacaa
      181 cacgatcgcg gcctaccttt taaacccgat taagaatgaa tatccgtatg aggacattgc
      241 caaggattat gcgggactga tgattctgtc gagaaaggat ctcatagaga agctcacgta
      301 tgagaaggcg gctgagaaaa agccggagga atttttaaag tgcgtctgct atatggcata
      361 cgtggcatac aagaccaggg agccgcttga aaaagagctt gcggacaccg gaatggaagc
      421 gctgtttcgc gagatcgaga tgccgcttgt cttcacgctg gcagatatgg aaaaagaggg
      481 aatcattgcg tccggggagg cgctaaagga gtacggagac aagcttgccg tgcgcattga
      541 cgaactcgag cgtaagatct atgaggaagc cggggaggaa ttcaatatca attctcccaa
      601 gcagctcggt gtgattttat ttgaaaagct ttccctgccg aacggcaaga agacgaagac
      661 cggctattcg acggcggcgg atgttttgga ccgtttggcg ccggattacc cgattgtggc
      721 ggatatcctg gagtacagac agctgaccaa gctaaagtct acttatgcag acggcctggt
      781 gaattatatc gcagaggacg gaagaatcca cacgtcattc aaccagacca tcacggcgac
      841 gggacgcctt agcagcacgg agccgaatct ccagaacatc ccgatgcgga ttgaactggg
      901 aagactgatc cgcaaggcat ttttgccaaa gaccggtttt gtgtttgtgg atgcggatta
      961 ttcgcagatc gagctgcgcg tgctggcgca tctgtccggg gatgagaagc tcatcgaggc
     1021 ataccggaat gcacaggata ttcaccggac gacggcgtcg caggtattcc atatcccgtt
     1081 tgacgaggtg accgatctgc agcgccgcaa tgccaaggcg gtcaatttcg gtatcgtcta
     1141 cggaatcagc tccttcggac tcagccagga tctctccatc acgaagaaag aggcggcgga
     1201 gtacattgag cgctattttg agacctatcc gaagatcaag gggtatctgg acggactggt
     1261 ggcggaggcg aaggaaaaag gctatgtcac gacgatgttc ggaagaagac gtccgattcc
     1321 ggagctttct tcgagcaatt ttatgcagcg ttcgttcggg gagcgcattg cgatgaattc
     1381 cccgatacag ggaacggcgg cagacattat taagatcgcc atgaaccggg tgcatgaccg
     1441 tctgctggcg gaggggttaa aatcccggct gattctgacg gtgcacgatg aactgctggt
     1501 ggagacagcg gcagaggaag aacaggaagt gcggaagatt cttgccgaag agatgcacgg
     1561 agcggcacag ttgtcggtga tgcttgagat tgatgtgcat gcgggaagcg attggtatca
     1621 ggcaaaatag aggtgcagtg gaacgatgag atttatagga attaccggcg gtgtaggagc
     1681 tggcaagtcg gaaatactgg gatatctggc gaagaagccg gatacccgcg tgatgcttgc
     1741 ggatgagatc gcccatgagc tgatgtctcc cggggcggag tgttatgaca ggatctgtga
     1801 gacgtttggc gcggaggata ttttcctgcc gcagggcgga ctggaccggg gaaaactggc
     1861 ggcagtcatt ttttccgatg aggcgaagcg caggcagatg aatgccattg tacacccggc
     1921 ggtgcgcgtc tatgtggaaa aagaggcggc gagagaaaag cagggtggaa aacgaaagct
     1981 tctggtgctg gaggcagcgc ttctgattga agaacattat gatgaaattt gtgacgaact
     2041 ctggtatatt tataccagag aggatattcg cagagaacgc ctgatggcaa gcagaggata
     2101 ttccacggaa aaggtagacc ggatttttgc aagccagttg acggaggaag tctaccggga
     2161 gcactgcagg gttgtgattg acaacaacgg cagtgtggcg gaggcttttg cgcagattga
     2221 cagggcgctt tcggagaaga aatagagatt aggagatact gcagatgagc gatacgagcg
     2281 tattcgacca gccgctggtg tttggtcttg atattggaac gagaaatgtg gtgggaaccg
     2341 taggctataa gacggagaat gaatttgtcg tggtggcaca gtatatgctt gagcatgaga
     2401 cgcgtgccat gctggacggt cagattcacg atatcggacg cgtggggcat accattgcca
     2461 aggtgaagga ggagctggag tcacagatcg ggcaggaact gaccgaagtg tgtattgcag
     2521 ccgcaggccg tgtgttaaag acggtgacga ccaacatcag ctacgatttc ccggaggaga
     2581 cggtcgttac cggcgaggat attcatacac tggatctgct tggaattgaa aaggcacagt
     2641 ccattttaaa ggaaatgaac gatacccgct acaagtttta ctgtgtgggt tattccgtca
     2701 tgaagtattt cttaaatgat gaaccgttct ccagtctgga atcccataag gcagagagga
     2761 tcagcgagga catcatcgtg acgttcctgc cggaggatgt tgtggacggt ctgtatgcag
     2821 cagttggcat ggcgggactg accgtggcaa acatgacgtt ggagcctatt gcggcgatca
     2881 atgtggcgat tccggagaat tacagactcc tgaatattgc gctcgtggat atcggtgcgg
     2941 gaacatccga tatttccgtg acgagagacg gaagcatcat cgcatacggt atgattcctc
     3001 ttgcggggga tgagatcacc gagctgatcg tgcagagtta tctggtggat ttcaacacgg
     3061 cggagcagat taagctctcg agcggcatgg aagatcaggt gacttataag gacatcatga
     3121 tgattgagca cacgattcca tcgaaggatg tgtggaag
//
LOCUS       DBMDQJ010000252         3154 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11137_length_3154_cov_1.556174, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000252 DBMDQJ010000000
VERSION     DBMDQJ010000252.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11137_length_3154_cov_1.556174"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="ACZO9R_06135"
     CDS             <1..818
                     /locus_tag="ACZO9R_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0912679.1"
                     /translation="SNQDQFYHIYKRLPNCIGEQAEGIDGGGTCAIPIEASDGSGRDV
                     FELYLFDSGTDAREGGYEAFDPKIIAWYRKQREDLREKNGMYVPSIVFQHIPMREYYE
                     VLKRVDRGEKGAVRAYRTHKNEYYKLGETCGAGDVFLEPPSVPDVNTGEFEALSECGE
                     VLAVFVGHDHKNNFVGCYRNIALGFTPSAGFNVYGNRTKRGVRCIVLNEKKPYEYHTY
                     TRTYEELVGKKVSRPVFDYLTSKAPATFEAAIPMILKAVLFLVLVIVLAVLLL"
     gene            892..1446
                     /locus_tag="ACZO9R_06140"
     CDS             892..1446
                     /locus_tag="ACZO9R_06140"
                     /inference="COORDINATES: protein motif:HMM:NF016060.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HIR0912680.1"
                     /translation="MGEIRQEKSGFAAWTDRHAELWKFMKFLLAGGGSNVVELAVHML
                     LLNTAFAALTAEPVTAHFLNLIGITSKGYLYTYMISTTVGYTIAFILNRKITFKADAN
                     PAVSMALYAVMVLFTIFANGWIGSAMTTWARENGWTGNLCDMVIKVIGMLIPMLWTYP
                     CNRFLIHRKRKDCELFHKKYNKRS"
     gene            1855..2100
                     /locus_tag="ACZO9R_06145"
     CDS             1855..2100
                     /locus_tag="ACZO9R_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0065007 - biological regulation [Evidence
                     IEA];
                     GO_process: GO:0065009 - regulation of molecular function
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM1266 family iron-only hydrogenase system
                     putative regulator"
                     /protein_id="HIR0912681.1"
                     /translation="METRIALIGIIVESTESVEQLNHLLHEYGSHIIGRMGIPYREKN
                     VHIISVAIDAPQDVINALSGKIGRLAGITAKTVYSNV"
     gene            2124..>3154
                     /gene="hydE"
                     /locus_tag="ACZO9R_06150"
     CDS             2124..>3154
                     /gene="hydE"
                     /locus_tag="ACZO9R_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HIR0912682.1"
                     /translation="MTHEEKIIEHLKTEKMLSVGELDCLLTTEDTETVEKLKAEARMA
                     AQSVYGKQIYIRGLIEFTNYCKNDCYYCGIRRGNACAERYRLTEEQILFCCATGYELG
                     FRTFVLQGGEDPYFTDEKICGLVSAIKRAYPDCAVTLSIGEKERASYQAYFDAGADRY
                     LLRHETADAVHYGKLHPEEMSLKNRMRCLRDLKEIGYQTGCGFMVGSPYQTTETLYED
                     LMFIKELAPAMVGIGPFIPQKDTPFGGETAGTLEMTLRLLSILRLMNPHVLLPATTAL
                     GTIHPQGREMGILSGANVVMPNLSPVSVREKYKLYDNKICTGDEAAECRFCMQRRMES
                     IGYEVVTDRG"
BASE COUNT          807 a          693 c          954 g          700 t
ORIGIN      
        1 tttccaatca ggatcagttc tatcatattt acaaaaggct tccgaattgc atcggggagc
       61 aggcagaagg gattgacggg ggcggaacct gtgccattcc catcgaggca tccgacggaa
      121 gcgggaggga tgtgtttgaa ctgtatctgt ttgacagcgg aacggatgcc agggagggcg
      181 gctacgaggc atttgatccg aagatcattg cgtggtaccg caagcagcgg gaagaccttc
      241 gggagaaaaa cggaatgtat gtgccgtcca tcgtatttca acatatcccg atgcgcgaat
      301 attatgaggt gttaaagcgt gtcgaccgcg gggaaaaagg tgcggtgcgc gcctatcgga
      361 cacataagaa cgaatattat aagctgggag aaacctgcgg cgccggggat gtttttctgg
      421 agccgccgtc ggtgccggat gtcaataccg gggaatttga ggcgctttcg gagtgtgggg
      481 aagtgctggc ggtattcgtc ggacatgatc acaagaacaa cttcgtgggg tgttaccgga
      541 acattgcgct tggatttaca ccgtccgcgg gatttaatgt ctacggcaac cgtacgaagc
      601 gcggcgtgcg ctgcatcgtg ctgaacgaaa aaaagccgta cgaataccat acgtatacca
      661 gaacgtatga ggaactggtc gggaaaaaag tgtcacgccc ggtatttgac tatctgacca
      721 gcaaagcacc tgccacgttt gaggcggcga ttccgatgat tttaaaggcg gtgctgtttt
      781 tggtacttgt gattgtgctg gcagtcttgt tgttgtagag ggatggggaa agcacccggc
      841 agaacaaggg cagcccggta agaaaaatac cgtgcagaaa aggggaaaac aatgggtgag
      901 atacgacagg agaaaagcgg atttgcggcg tggaccgaca gacatgcgga actgtggaag
      961 tttatgaaat tcctgcttgc gggaggcggt tccaatgtgg ttgagctggc ggtgcatatg
     1021 ctgcttttaa atacggcatt tgctgcgctt acggcggaac cggtaaccgc gcattttctc
     1081 aacctgatcg gcattacgtc gaagggatat ctgtatacct atatgatctc gacgaccgtg
     1141 ggttatacga ttgcctttat attgaaccgc aagattactt ttaaggcgga cgccaatccg
     1201 gcggtcagca tggcactcta tgcggtcatg gtactgttca cgatttttgc gaacggctgg
     1261 atcggatcgg cgatgacgac ctgggcaagg gaaaacggct ggacggggaa tttgtgcgac
     1321 atggtaatca aggtgatcgg aatgctgatt ccgatgctct ggacctatcc gtgtaaccgg
     1381 tttctgatcc accggaaaag aaaagattgt gaattgtttc acaaaaaata caacaaaaga
     1441 tcttgaacgg gaacagactt catgatacgc tgaaacagca ttgcagtgag atacgttctt
     1501 gctgtcttgc cttaacaaaa gtctctgacg ccaaaagcag tgcctgcggc ggaagatatg
     1561 gcggtcatga aagtgtctgc atctggtctg cgcgtgcgga acagatgctg tagataggat
     1621 accggcggga tattccatgc cttgtagggc aggttctgcc gtgtatttcc agcgagtgcc
     1681 gtatctgaga ggagatttca gatacggttt ttttatgtaa ataggaaact tcgttcctat
     1741 tacataaaaa gctcttcggg gatcgcactg cggcggagac gaaagatgtc gcaagagcga
     1801 cccgccgcag gcggagaagc ctgcaggcag gattctttct tattaggaga aaatatggag
     1861 acaagaattg cacttatcgg tatcattgtg gagagcaccg agtccgtgga gcagctcaat
     1921 catctgctgc acgaatacgg aagccatatc atcggcagaa tgggaatccc ttaccgggaa
     1981 aaaaatgttc acattatcag cgttgccatc gatgcaccgc aggatgtcat caatgcactg
     2041 tcgggaaaga tcggacggct ggcgggaatc acggcaaaga ccgtctattc caatgtataa
     2101 gcgtctgcgg aatggagatt tatatgacac acgaggaaaa aatcatagag cacctgaaga
     2161 cggagaagat gctgtcggtg ggtgaactgg actgtctgct tacaacagaa gatacagaga
     2221 cagtcgagaa gcttaaggct gaggcgagaa tggcggcaca gtcggtatac ggaaaacaga
     2281 tctatatccg cgggctgatc gagttcacca actactgtaa aaatgactgc tattactgcg
     2341 gcatccgcag gggcaatgca tgtgccgagc gctaccgcct cacagaggag cagatcctct
     2401 tctgctgcgc gacagggtat gagcttggat tccgcacctt tgtcctgcag ggcggagaag
     2461 acccctattt tacagatgag aagatctgcg gtctggtgtc tgcgatcaag cgggcatacc
     2521 cggactgtgc agtgacactc tcgatcgggg aaaaagaaag agcgagttat caggcatatt
     2581 ttgacgccgg cgcagaccgg tatctgctta ggcacgagac ggcggatgcg gtacactacg
     2641 ggaagctgca cccggaggaa atgtccctta aaaaccggat gcgctgtctg cgggatctaa
     2701 aggagatcgg ttatcagacc ggatgcggct ttatggtggg ctcgccgtat cagaccacgg
     2761 agacgctgta tgaagacctg atgtttatta aggaactcgc gcctgccatg gtgggaatcg
     2821 gaccgttcat tccgcagaag gacacgccgt ttggcgggga gactgcgggg acgttggaga
     2881 tgaccctgcg gctgctttcc atcctgcggc tgatgaatcc gcatgtgctg cttccggcga
     2941 cgacggcgct cggcacgatc cacccgcagg gaagggaaat gggaattctc tccggcgcca
     3001 atgtggtgat gccgaatctc tcgccggttt ccgtgaggga aaaatataag ctttacgaca
     3061 ataagatctg cacgggggat gaggcggcag aatgtcgttt ctgcatgcag aggcggatgg
     3121 agagcatcgg ctatgaagtg gttaccgacc gcgg
//
LOCUS       DBMDQJ010000253         3150 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11158_length_3150_cov_1.412266, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000253 DBMDQJ010000000
VERSION     DBMDQJ010000253.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11158_length_3150_cov_1.412266"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /locus_tag="ACZO9R_06155"
     CDS             <1..1707
                     /locus_tag="ACZO9R_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR0912683.1"
                     /translation="ELAEKSGDPKAAEDDEVTFTCPDCGKTMKRVPEVIDCWFDSGAM
                     PFAQHHYPFENQDLFAQQFPAKFISEGVDQTRGWFYSLMAESTLLFNKAPFENVIVMG
                     HVQDENGQKMSKSKGNAVDPFEALSAYGADAIRWYFYTSSAPWIPKRFNGKLVQEGQR
                     KFMGTLWNTYAFFVLYANIDNFDASKYVLAYEKLSVMDKWLLSKLNTVIKEVDNNLSN
                     YRIPEAARALDDFVDDMSNWYVRRSRERFWAKGMEQDKINAYMTLYTALVTICKCAAP
                     MIPFMTEDIYQNLVRSVDQTAPESIHLCDFPVANEVYIDAELEKNMDEVLKIVVMGRA
                     CRNSANIKNRQPIGNMFVKAPSALPEFFVEIIEDELNVKKVTFTDDVSAYTSYTFKPQ
                     LRTVGPKYGKFLGQIQKALAALDGNAAMAELKANGVLALPSVDESVKLAEEDLLITMT
                     QMEGYVTEGDNTVTVVLDTNLTPELIEEGFVREIISKIQTMRKEAGFEVMDQITIYHK
                     SDEKVSGIFEQYGDTIKSDVLGVNVVADAAGAFADEPEVYSKEWNINGEHVLLGVKKG
                     GN"
     gene            1708..>3150
                     /gene="glgP"
                     /locus_tag="ACZO9R_06160"
     CDS             1708..>3150
                     /gene="glgP"
                     /locus_tag="ACZO9R_06160"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HIR0912684.1"
                     /translation="MKSGVFDKKAFIQSVRDNLKNLYRKTLEEADQQEIFQAVSFAVK
                     DVINDRWLATQQVFDKEDPKIVYYMSMEFLMGRALGNNLINLKEYKEVREALDEIGLN
                     LDVIEDQEPDPALGNGGLGRLAACFMDSLSTLGYAAYGCGIRYRYGMFKQKIQDGFQV
                     EVPDNWLKNGYPFELHRPEYTYEIKFGGHVRTESREDGSLRFVQEDYQSVLAIPYDMP
                     VVGYGNNVVNTLMIWDAAAKDYFELDSFDKGDYQKAVEQQNLARNLVEVLYPNDNHVA
                     GKELRLKQQYFFVSASVQRALARYKKNHDDIRRLPEKVTFQLNDTHPTVTVAELMRIL
                     VDEEGLGWDEAWEITTKTCAYTNHTIMAEALEKWPIEIFQRLLPRIYQIVDEINRRFV
                     LQIQERYPGDDGKVARMAILYDGQVKMAHLAIVAGYSVNGVARLHTEILKNEQLHDFY
                     EMFPEKFNNKTNGITQRRFLMHGNPLLADWV"
BASE COUNT          827 a          688 c          924 g          711 t
ORIGIN      
        1 gagcttgcag agaaaagcgg cgatccgaag gctgcggagg acgatgaagt gacctttacc
       61 tgcccggact gcggcaagac catgaagcgt gtgccggaag ttatcgactg ctggtttgat
      121 tccggtgcga tgccgtttgc acagcatcac tatccgttcg agaatcagga tctgtttgcg
      181 cagcagttcc ctgccaaatt catttccgag ggtgtggatc agacccgtgg atggttctat
      241 tccctgatgg cggagagtac actgttattt aacaaggctc cgtttgagaa tgttatcgtc
      301 atgggacatg tgcaggacga gaacggacag aagatgagca aatccaaagg aaatgcggta
      361 gacccgtttg aggcgctctc tgcctacggt gcagacgcga tccgctggta tttctacacg
      421 agcagtgcgc cgtggattcc gaagcggttt aacggcaaac tcgtgcagga gggacagcgc
      481 aagttcatgg gtacgctctg gaatacctac gcgtttttcg tgctgtatgc gaacattgac
      541 aattttgatg catcaaaata tgtgcttgcc tatgagaagc tttccgtgat ggacaaatgg
      601 ctgctctcca agctgaatac ggtgattaag gaagtggata acaacctttc caattaccgc
      661 attccggagg ctgcaagagc gctggatgat ttcgtcgatg acatgagcaa ctggtacgtc
      721 agaagaagcc gtgagcgttt ctgggcaaag ggcatggagc aggataagat caacgcgtac
      781 atgacactct acacagcgct tgtgacgatc tgcaagtgtg cggcgccgat gattccgttt
      841 atgacagagg atatctatca gaatctggtg cgctccgtgg atcagacggc tccggagagc
      901 attcatctgt gcgattttcc ggtggcaaac gaggtgtaca tcgatgcaga gcttgagaaa
      961 aatatggatg aggtcttaaa gatcgttgtc atgggccgtg catgccgtaa ttccgcgaac
     1021 atcaagaacc gtcagccgat cggaaacatg ttcgtaaaag ctccgtctgc actgccggag
     1081 ttcttcgtgg agatcatcga ggatgagctg aatgtgaaaa aagtgacatt tacagacgac
     1141 gtgagcgctt acacaagcta cacgttcaag ccgcagctgc gcaccgtcgg accgaagtac
     1201 ggcaagttct taggacagat ccagaaagca ctcgcagcgc ttgacggcaa tgcggcgatg
     1261 gcagaattaa aggcaaacgg cgtgcttgca cttccgtccg ttgacgagtc tgtcaagctg
     1321 gcagaggagg atctcctgat caccatgacg cagatggaag gttatgtgac cgagggagat
     1381 aacaccgtga cagtggtgct cgatacgaac ctgacaccgg aacttatcga ggagggcttt
     1441 gtgcgtgaga tcatcagcaa gatccagacc atgcgtaagg aagccggatt cgaggtgatg
     1501 gatcagatca cgatctatca caagtcagat gaaaaagtat ctggtatttt tgagcagtat
     1561 ggtgatacaa ttaaatcgga cgttctcggc gtgaatgtcg tggcggatgc agccggagca
     1621 tttgcggatg agccggaagt ttacagcaag gaatggaata ttaacggcga acatgttctt
     1681 ctgggagtga aaaaaggagg aaactagatg aaatcaggtg tttttgacaa gaaagccttt
     1741 attcagagtg tgcgcgataa tctgaagaat ttataccgca agacattgga ggaggcggat
     1801 cagcaggaaa ttttccaggc ggtctccttc gcggtcaagg atgtcatcaa tgacagatgg
     1861 cttgcaacac agcaggtatt tgacaaggaa gatccaaaga tcgtgtacta catgtcgatg
     1921 gaatttctga tgggacgcgc gctcggcaat aatctgatca atctgaagga atataaagaa
     1981 gtgagggaag cgctggatga gatcggcctg aatctcgatg tgatcgaaga tcaggagccg
     2041 gatccggcac tcggcaacgg tggtctggga agactggctg cctgctttat ggattccctc
     2101 tccacactcg gatacgcagc ttacggctgc gggatccgtt atcgctacgg tatgttcaag
     2161 cagaagattc aggacggctt tcaggtcgaa gtgcctgata actggctgaa aaatggttat
     2221 ccgtttgaac tgcacagacc ggagtacact tacgagatca agtttggcgg acatgtacgc
     2281 acggagagca gggaggatgg aagcctgcgt tttgtgcagg aggattatca gtcggtgctg
     2341 gcgatcccgt atgacatgcc ggtggtcgga tacggcaaca acgtggtcaa tacgttaatg
     2401 atctgggatg cggcggcaaa ggattatttt gagctggatt catttgataa gggagattat
     2461 cagaaggcgg tcgagcagca gaatcttgca agaaatctgg tggaagtgct ttacccgaac
     2521 gacaatcatg ttgcgggcaa ggagctccgc ttaaagcagc agtatttctt cgtatctgcg
     2581 agtgtacagc gcgcgctggc gcgttacaag aagaatcatg atgacatccg cagactgccg
     2641 gagaaggtga cgttccagtt gaacgacaca cacccgacgg tgacggtggc agagctgatg
     2701 cgtattcttg tggatgagga aggtctcggc tgggacgagg cgtgggagat taccacgaag
     2761 acctgtgcct acaccaacca tacgatcatg gcggaagcac tagaaaaatg gccgattgag
     2821 atttttcagc ggctgcttcc gagaatctac cagatcgtgg atgagatcaa ccgccgtttt
     2881 gtactgcaga tccaggaacg ctacccgggg gatgacggca aggttgcccg catggcaatc
     2941 ctttacgacg gtcaggtgaa gatggcgcat ctcgccattg tagccggtta ttccgtcaac
     3001 ggtgtggcaa gactgcacac ggagatctta aagaacgagc agcttcatga tttttatgag
     3061 atgttcccgg agaagttcaa caacaagacc aacggcatca cgcagagacg tttcctgatg
     3121 cacggcaatc cgctgcttgc cgactgggtg
//
LOCUS       DBMDQJ010000254         3137 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11211_length_3137_cov_1.584321, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000254 DBMDQJ010000000
VERSION     DBMDQJ010000254.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3137)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3137)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3137
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11211_length_3137_cov_1.584321"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            132..1328
                     /gene="glf"
                     /locus_tag="ACZO9R_06165"
     CDS             132..1328
                     /gene="glf"
                     /locus_tag="ACZO9R_06165"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HIR0912685.1"
                     /translation="MKQVNVKTMRAVPQNGGDMYDCIVIGAGIAGAVAARKLAEEKGK
                     RVLVMERRPHIGGNCYDEKDAHGILIHNYGPHIFHTGLEEVFAYLSRFTDWYLFGHEV
                     VAKVGDQLIPVPFNLNTLHMVYRKEKADRLEQKLIETYGEGSRVPIMKLRENEDADIR
                     EIAQYVYENVFLYYTMKQWGQKPEEISPEVTGRVPVLISYDNRYFQDKYQGVPANGFT
                     EMFEKMLSHPGITVGCGTDCLSRMRFADNEIYFDGEKFDGDVIYTGALDELFACRYGR
                     LPYRSLDFRFEHYDGASYQGHSVVNYTVSEDFTRITEFKFLTGQKDTDGTTIVKEYPF
                     AYTGAEGEIPYYAILNEENQALYEKYRALTAGMEHFHLLGRLAEYRYYNIDAMVKRAM
                     ELADRL"
     gene            1403..2416
                     /locus_tag="ACZO9R_06170"
     CDS             1403..2416
                     /locus_tag="ACZO9R_06170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912686.1"
                     /translation="MKLLTFAIPCYNSEAYMEKCIESLLPGGDDVEILIVDDGSHDRT
                     AEIADAYEKKYPGIIRAIHQENGGHGEAVNTGIRNATGLYFKVVDSDDWVDLDAYRKI
                     LDKLREISGGDRVLDMFIANYVYEKEGVKHKKVMRCSNLPKDRIFTWKEAGHFHKGQY
                     ILMHSVIYRTKLLVECGLELPKHTFYVDNIYVYKPLPSVRTLYYMDVDFYRYFIGRDD
                     QSVNERVMISRIDQQIRVNKIMVDAFDLWKIPNRKLRHYMFNYLEIITVISTIMLIRS
                     GTEENLEKKRELWKYIKEKDIRLFHHLRAGILGNTMNLPGKGGRKISVAAYKLSQKVV
                     GFN"
     gene            complement(2984..3136)
                     /locus_tag="ACZO9R_06175"
     CDS             complement(2984..3136)
                     /locus_tag="ACZO9R_06175"
                     /inference="COORDINATES: protein motif:HMM:NF037014.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912687.1"
                     /translation="MEIREICNHEKDTIWDETKRFVNPQEIYVDLSQKLYDMKTALLN
                     EMSMDS"
BASE COUNT          845 a          633 c          883 g          776 t
ORIGIN      
        1 cagcgtatgc caagtttatt agtattttgt aaataaaata tgaacaaatg aaggaagtgg
       61 acatttttca gtggatattt tgaaaacctt aaggaaaaaa taaattggcg tactgtggaa
      121 aatcctgtat aatgaaacag gtcaatgtta agacaatgcg ggcagtaccg cagaacggag
      181 gagacatgta cgattgtatt gtcattggag ccggaattgc cggagcggtg gcagcgcgaa
      241 agctggcaga ggaaaaagga aagcgtgtcc ttgtcatgga acgccgaccg catatcgggg
      301 gaaactgtta cgatgagaaa gatgcccacg gcattttaat tcataattac ggaccgcata
      361 tttttcacac aggtctggag gaagtgtttg catatttatc gcgttttacg gactggtacc
      421 tgttcgggca cgaggtggtc gcaaaggtcg gggatcagct gattccggtg ccgtttaact
      481 taaatacgtt acatatggta tacaggaagg agaaagccga ccggctggag caaaagctga
      541 tcgaaaccta cggcgagggt tcgcgtgtgc cgatcatgaa gctgcgcgag aacgaggatg
      601 cggatatccg cgagattgca cagtatgtgt atgagaatgt atttttatat tacacgatga
      661 agcagtgggg gcagaagccg gaggagatca gtccggaggt cacagggcgt gtcccggtgt
      721 taatctccta tgataaccgc tattttcaag ataagtatca gggagtgccg gcgaacggat
      781 ttacggaaat gtttgagaag atgctttccc atccggggat cacggtgggg tgcggcacgg
      841 actgcttaag ccgcatgcgc tttgcggaca atgagattta ttttgacggc gaaaaattcg
      901 acggcgacgt gatctacacc ggcgcgctgg atgaattgtt tgcatgcagg tatggaagac
      961 tgccgtaccg ctcgctggat ttccggtttg agcattacga cggggcgtct taccagggac
     1021 acagtgtggt caactacacg gtgagcgagg attttacccg tatcacggaa ttcaagtttt
     1081 tgaccgggca gaaggatacg gacggcacca cgatcgtgaa ggagtatccg tttgcgtata
     1141 cgggagcaga gggagagatt ccgtactatg cgattttgaa tgaggaaaat caggcgctgt
     1201 acgaaaaata ccgggcgctc acggcgggca tggagcattt tcatctgctc ggacggctgg
     1261 cggagtaccg ctattacaac atcgacgcga tggtgaagcg cgccatggaa ctggcagaca
     1321 gattgtaaga gtgtgacgga aaatctgcct tttgggaaga aagccgggtt ttcttttgcg
     1381 gagataaagg agagattaga aaatgaaatt gttgacattt gcgattccgt gctacaattc
     1441 ggaagcatat atggaaaagt gtatcgagtc gctgcttccg ggcggggatg atgtggagat
     1501 tttgatcgtg gatgacggat cgcatgaccg cacggcagag attgcggatg cgtatgagaa
     1561 aaagtatccg gggattatcc gggcgattca tcaggagaac ggcggccacg gcgaggcggt
     1621 caatacgggt atccgcaatg ccaccgggct ttattttaag gtagtggaca gtgatgactg
     1681 ggtggatctg gacgcctacc gcaagattct ggataagtta cgggagattt ccggcggtga
     1741 cagagtgctg gatatgttta ttgctaatta tgtatatgag aaagagggag tgaagcataa
     1801 aaaagtcatg cgctgctcca atctcccgaa agaccggata tttacatgga aggaagcagg
     1861 acatttccat aagggacagt atattctcat gcattccgtg atctaccgca cgaagcttct
     1921 ggtggaatgt ggactggaac tgccgaagca caccttctat gtggataaca tctatgtata
     1981 caagccgctt ccgagtgtgc ggacgctcta ttatatggat gtggattttt accggtattt
     2041 tatcggaaga gacgatcagt ccgtcaatga gagagtgatg atttcccgca ttgaccagca
     2101 gatccgtgtg aataagatta tggtcgatgc gtttgacctg tggaaaattc caaaccgcaa
     2161 gctcagacat tatatgttca actatctgga gatcatcacg gtcatctcga cgatcatgct
     2221 gatccgctcc ggcacggagg agaatctgga gaagaagaga gaactctgga aatatattaa
     2281 ggaaaaggat attcgcctgt tccaccatct gcgtgcgggt atccttggca atacgatgaa
     2341 tctgccggga aaaggcggaa gaaagatctc cgttgcggcg tataagcttt cacagaaagt
     2401 ggtcggattc aactagcggg aatccctgca ctgtatttgt gagagattag ttcggacaat
     2461 gcatggtatg aagttccaga atgtaaccgg cggcagcatt ctttgaagca aaattcgagt
     2521 gtttcttcca tgccgcgtcc ggtatccaat tctgtgtctt tgccacgctt tgacaatcca
     2581 cagggtgtgt ccgacaaccc gcagacaatg agcggacttt ctagtggcgc taatgccact
     2641 tcttcacaga caatataaaa ccgcgcgaac cgaaactcgt tggcatctgc tattgaaaat
     2701 aaggcagatg tcaattcaaa catcggcttc gcgcggttat gttttgttca tcagtggcaa
     2761 taagcgccac acaaaagtcc taaaattgtc tgccggcttg cggacacatc ctgcggagtg
     2821 ttcaaagctt ggaaagacac ggatcatcaa agatgccgga cgcggtgtgg ttagaaatac
     2881 cggattttgc ggtcagaatg ctgctgacgg ttacagctgg aactttatac acatgcatta
     2941 tccgaaaaag ttctctctgg tacagcccag ggattgcctg ctattaagaa tccatactca
     3001 tttcgtttaa gagagccgtt ttcatatcgt agagcttctg ggagaggtcg acgtagatct
     3061 cctgcgggtt gacgaaacgc ttggtctcat cccagatggt gtctttttcg tgattacaga
     3121 tttcacggat ctccatg
//
LOCUS       DBMDQJ010000255         3136 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11215_length_3136_cov_1.636573, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000255 DBMDQJ010000000
VERSION     DBMDQJ010000255.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11215_length_3136_cov_1.636573"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..626
                     /locus_tag="ACZO9R_06180"
     CDS             <1..626
                     /locus_tag="ACZO9R_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0912688.1"
                     /translation="RSGIEDYYLFLFVALVPWIFFSSCVTGGATSILNSGDMIKKIYF
                     PREVMPIAYVTSAFVNMILTFVVVFAVLIVSGYGMNARALLYLPIIMIVEYLLGLGIA
                     LLTSALTVFFRDLAYILGIVAMAWQFLTPVMYSQEMVEEALPAGMLKIWNMNPMTPMI
                     NAYRDILYYKTTPQLSTLAVAVVLGIVILVIGEIVFVKLQRGFAEEL"
     gene            652..1107
                     /locus_tag="ACZO9R_06185"
     CDS             652..1107
                     /locus_tag="ACZO9R_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912689.1"
                     /translation="MVEQMEFVDVEGIMSEIRTQIKERGYTKKDLKFADMNTSAVLGA
                     EDYYDYPNFRATLEQVDGKRYVPWWNPIKGNKVVVFIKRFIRKCVTFLIAPIVDAQNE
                     LNQYTASALVQIGAKFEQEQDYDYDEMQKKIEMLEQRIAELEKSVENNR"
     gene            1104..2183
                     /locus_tag="ACZO9R_06190"
     CDS             1104..2183
                     /locus_tag="ACZO9R_06190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912690.1"
                     /translation="MKIFQILPVLAFGDAVGNDTRALKDALLEAGYQTEIYAAVIDER
                     LPEGTAQSFDMMEKVEEDDIIIYHLSTGHELNQKLKSLGGRKIILYHNITPEKYFQKY
                     NHGAYLNCRNGLREARELAPVAEFAIADSEYNKQDLIRYGYTCDIKVLPILIPFEDYE
                     KKPNQKVINKYGDDGYVNILFTGRVVPNKKQEDIIDAFYYYKKFINPKSRLILVGSYA
                     GIDRYHEQLEAYVKALDLEDVLFTGQIKFDEILAYYKVADVFLCMSEHEGFCVPLLEA
                     MCFDVPVIARDTSAIAGTLGGSGVLLPDNDPMVAAEMINRIVTDEKLRETIIRNQRIR
                     LKDFDNRVVKETFLKIIREFIGKRD"
     gene            2186..>3136
                     /locus_tag="ACZO9R_06195"
     CDS             2186..>3136
                     /locus_tag="ACZO9R_06195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0912691.1"
                     /translation="MKKIGFVIPWFGFDIPGGAEAELKGLTLHLHEAGVPIEILSTCV
                     QNFNANWNCNYHKEGTTTEKGITVRRFKVRKRDADAFNEVNLKLMNNQMPTEAEEEIF
                     NREMVNSPALYRYIREHKDEYSLFVFIPYMFGTTYYGIQECYEKAVLIPCFHDESYVY
                     MKSFKREFSKVAGMIFHAKPEGDLAEQVYDLTNVNAQVLGEGVYTEYTYDAARFRDKY
                     NIREPFILYAGRKDVGKNIYTLINNFREYKYRNQNDMKLVLIGGGEVKIPEDIKKDVI
                     DLGFVDVQDKFDAYAAATVLCQPSTHESFSLVIMESWICER"
BASE COUNT          901 a          575 c          853 g          807 t
ORIGIN      
        1 tgagatcggg aattgaggat tactatctat tccttttcgt ggcgcttgtt ccatggatct
       61 tcttcagctc ctgcgtgact ggcggtgcga cgagcatttt gaattccggt gatatgataa
      121 agaagattta ttttccgaga gaggtaatgc cgatcgctta tgtcaccagt gcgtttgtca
      181 acatgattct cacatttgtg gtggtgtttg cggtactcat tgtctcgggc tatggaatga
      241 acgcgcgggc actgttatat ctgcccatca ttatgattgt ggagtatctg ctcggacttg
      301 gaatcgcgct tctgacgtct gcgcttaccg tatttttccg tgatctggca tatattcttg
      361 gcatcgttgc gatggcgtgg cagtttttga caccggtcat gtattcacag gagatggtgg
      421 aggaggcatt gcctgccggc atgttaaaaa tctggaatat gaatccgatg acgccaatga
      481 tcaatgccta tagagatatt ctatactata agacgacgcc gcagcttagc accctggctg
      541 ttgcggttgt acttggaatc gtgatactgg tgatcggaga aattgtattt gtaaagctgc
      601 agagaggctt tgcggaagaa ctgtagagaa gataatggag gtaggcatac aatggtagag
      661 cagatggaat ttgtggatgt agaaggaatc atgagtgaga tccggacaca gatcaaagag
      721 cgtggatata caaagaaaga tttgaaattt gcggatatga acacgagtgc ggtgctgggc
      781 gctgaggatt attatgacta tcctaatttt agggcgacgc ttgagcaggt tgacgggaag
      841 agatacgttc cgtggtggaa tccgattaag ggaaataaag tagttgtctt tatcaagaga
      901 ttcataagaa aatgtgttac attcctgatt gctccgattg tggatgcaca gaacgagttg
      961 aatcagtata cggcatcggc actggtacag atcggcgcaa agttcgagca ggaacaggac
     1021 tatgattacg acgagatgca gaagaagatc gagatgttgg aacagcgcat tgctgaatta
     1081 gaaaaaagtg tggagaataa tcgatgaaaa tttttcagat attaccggta cttgcctttg
     1141 gcgatgctgt tggaaacgat acgagagcat taaaagatgc actgctggag gcaggctatc
     1201 agacagagat ctacgctgcg gtaatcgacg agcgtttgcc ggagggaacg gcgcagtcgt
     1261 ttgatatgat ggagaaggtg gaagaagatg acattattat ttatcatctc tctaccggtc
     1321 acgaattaaa tcaaaagtta aagagtcttg gcggaagaaa aattattctt tatcacaata
     1381 ttactccgga aaaatatttt caaaaatata atcacggagc ctatctgaac tgccggaacg
     1441 gactgcggga ggcaagagaa ctggcaccgg tggcggaatt tgccatcgca gattcagaat
     1501 acaacaagca ggatctgatc cgttacggtt atacctgcga catcaaagtg cttccgattc
     1561 tgatcccgtt tgaggattat gagaaaaagc cgaatcagaa agtcataaat aaatacgggg
     1621 atgacgggta tgtgaatatc ctttttacag ggcgcgttgt tccaaacaaa aaacaggaag
     1681 acattattga tgcgttttac tactataaaa aatttatcaa tccaaaatca agactgattc
     1741 ttgtcggctc ctatgcgggc attgaccgct accatgagca gctggaagcc tatgtaaagg
     1801 cgcttgatct ggaagatgtg ctcttcaccg gacagattaa gtttgatgag attttagcat
     1861 actacaaagt ggcggatgtt ttcctgtgca tgagcgagca tgagggattc tgtgtaccgc
     1921 tgttggaggc gatgtgtttt gacgtgccgg tcatcgcgcg cgacacttcc gcgattgcgg
     1981 gaacacttgg gggaagcggc gtgctccttc cggataatga cccgatggtg gcggcggaaa
     2041 tgatcaaccg tatcgtcacg gacgaaaagc ttagggagac gatcatacgc aatcagagaa
     2101 tccgtctgaa ggattttgat aaccgtgtgg taaaagagac atttttaaaa attatcagag
     2161 agtttatagg caagagggat tgattatgaa gaaaatcgga tttgtcatac cgtggtttgg
     2221 atttgatatt ccgggcggcg ctgaggcaga gctaaagggg ctgaccctgc atctgcatga
     2281 ggcaggcgtg ccaatcgaga ttttatcgac atgtgttcag aattttaatg caaactggaa
     2341 ctgcaattat cataaagagg ggacaaccac ggaaaaaggg attaccgtac gcagatttaa
     2401 agtgcgcaag cgggatgcgg atgccttcaa cgaggtgaat ctcaagctta tgaacaatca
     2461 gatgccgacg gaggcggaag aagaaatttt caaccgtgag atggtgaaca gtccggcatt
     2521 atacagatat atcagagaac acaaggatga gtacagcctg tttgtgttca ttccgtatat
     2581 gtttggaacg acctactatg gaatacagga gtgctatgaa aaggcggtct taattccgtg
     2641 cttccatgat gaaagctatg tatacatgaa gtctttcaag cgggaattct ccaaagtggc
     2701 gggcatgatt ttccatgcga agccggaagg cgatctcgcg gagcaggtct atgatctgac
     2761 caatgtgaat gcacaggtgc tcggagaggg cgtgtataca gagtatacct atgatgcagc
     2821 gcgcttccgc gacaaataca acatcaggga accgtttatt ttatatgcgg gaagaaaaga
     2881 cgtcgggaaa aatatctata ccctgatcaa taatttccgc gaatataagt acagaaacca
     2941 gaatgacatg aagctggtat tgatcggcgg cggcgaggtg aaaatcccgg aggatatcaa
     3001 aaaagatgtg atcgatctgg gctttgtgga tgttcaagac aaattcgacg cttatgcagc
     3061 agcaaccgtg ctgtgccagc cgtcgacgca tgagagcttt tccctggtaa tcatggagag
     3121 ctggatctgt gagaga
//
LOCUS       DBMDQJ010000256         3133 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11228_length_3133_cov_1.433047, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000256 DBMDQJ010000000
VERSION     DBMDQJ010000256.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3133)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11228_length_3133_cov_1.433047"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            122..194
                     /locus_tag="ACZO9R_06200"
     tRNA            122..194
                     /locus_tag="ACZO9R_06200"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:155..157,aa:Thr,seq:cgt)
     gene            314..>1355
                     /locus_tag="ACZO9R_06205"
     CDS             314..>1355
                     /locus_tag="ACZO9R_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR0912692.1"
                     /translation="MKKKVISLLLTAAMATTMFAGCGSSSEENTTADNNQTAAPAATE
                     AAAEESTEAAAPAVSADGKIAMITDSGDITDESFNQITWETCVAYGDKNGIEYQYYKP
                     AEDTDEERINAIDLAVADGATVIVMPGYLFGPAIAEEQDLYPDVSFIAVDVTEADIVD
                     LSGNAVGISDNVYICSFQEEQAGYLAGYAAVKDGYTSLGFLGGIAVPAVIRYGYGYIQ
                     GINDAAEEMGVDVDVKYYYGGQFYGSDAITARMEGWYQDGTQVVFACGGGIYTSAVEA
                     AEQYDGKVIGVDVDQRPKIGDLCITSAMKGLGSAVNSALDAYFGGNWASIGGKSEQLG
                     LAQGDYLGLPTDT"
     assembly_gap    1356..1365
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1632..>3133
                     /locus_tag="ACZO9R_06210"
     CDS             1632..>3133
                     /locus_tag="ACZO9R_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912693.1"
                     /translation="MYMENEYVIEMLNITKEFPGIKANDNITLQLRKGEIHALLGENG
                     AGKSTLMSILFGLYQPTSGTIKKNGQVVQINTPNDANDLNIGMVHQHFKLVECFSVLD
                     NIILGVEPSKGLFLEKDGARKKVLELSERYGLKVEPDALISDITVGMQQRVEILKMLY
                     RDNEVLIFDEPTAVLTPQEIEELMKIMKNLAHEGKSILFITHKLNEIMEVADRCTILR
                     KGKYIGTVDIADTTKEELSKMMVGRNVSFTVDKDEKKPGDTVLEVSHLTVASRSHENN
                     AVRDVSFQVRRGEIVCIAGIDGNGQTELVQGLTGLEKIGGGSVKFLGQDITNTTIRER
                     SKHGMSHIPEDRHKHGLVLDYTLEQNLVLQRYWQPQFERAGFIKSGEVRRYAQKLIEQ
                     YDVRSGQGPVTIVRSMSGGNQQKAIIAREIDKDPELLVAVQPTRGLDVGAIEYIHKQL
                     VAQRDKGKAVLLVSLELDEVMDVSDRILVMYEGEIVGELDPKKVTVEELGL"
BASE COUNT          857 a          613 c          905 g          748 t
ORIGIN      
        1 tatgctgatt ttgcagtatt ttcatagcat aagaaagatt ttattttaat aattgaaaaa
       61 aagtagttgc aattttttga aattccgtta taataactca atgtgatgtt aaacatgacc
      121 tgccgatatg gctcagttgg tagagcaacg cattcgtaat gcgtaggtcg tcggttcgag
      181 tccgactatc ggctttaaat aaaaactctc agtaattgtg ctgggagatt tttatttata
      241 tgctagttat aacacatgct tatcaatgtt tattagagcg ggttatgact gagtgcaaaa
      301 ggaggaaaat attatgaaga aaaaagtgat tagcttactg ctcactgcag caatggcaac
      361 caccatgttc gcaggctgtg gttcttccag cgaggagaat acaactgcag acaacaatca
      421 gacagcagca ccggcagcga cagaggctgc agcagaggag agcacagagg cagctgctcc
      481 tgcagtatcc gctgatggaa agatcgcaat gattaccgat tccggtgaca tcacagacga
      541 gtctttcaac cagatcacat gggagacctg tgtggcttac ggtgataaga acggtattga
      601 gtatcagtac tataagccgg ctgaggacac agacgaggag agaatcaacg caatcgacct
      661 tgcagttgca gacggtgcaa ccgtaatcgt tatgccgggt tatctgttcg gaccggcaat
      721 cgctgaggag caggatcttt atccggatgt ttcctttatc gcagttgatg tgacagaggc
      781 tgacatcgta gatctttccg gcaacgcagt cggaatcagc gacaacgtat atatctgttc
      841 tttccaggag gagcaggcag gttaccttgc aggttatgcg gcagttaagg atggttacac
      901 aagccttgga ttcctcggtg gaatcgcagt tccggcagtt atccgttatg gttacggtta
      961 tattcagggt atcaacgatg cagcagaaga gatgggcgtt gatgtagatg tgaaatacta
     1021 ctatggcggt cagttctacg gaagtgatgc gatcacagct cgtatggagg gctggtatca
     1081 ggatggcaca caggttgtat tcgcatgcgg cggcggtatc tacacatctg ctgttgaggc
     1141 tgcagagcag tacgacggca aggtaatcgg tgtcgatgtt gaccagcgtc cgaagatcgg
     1201 tgatctgtgc attacctctg ctatgaaggg cttaggctcc gcagtgaact ctgcactgga
     1261 tgcatacttt ggcggcaact gggcttccat cggtggcaag tctgagcagc tcggtctggc
     1321 acagggcgat tatcttggtc ttccgacgga tacagnnnnn nnnnnctggg gctttacaac
     1381 atttacggta gatgagtaca atacagtatt agacggactc aaggacggca gcatcactgt
     1441 ttctaacgat acagagaatc agccggaggt aggttctcac gtgaccgttg actatgtaga
     1501 gtaagcggac tctttaaagg ggaagagaaa tagtttctct tcctcttttt tttatatcca
     1561 gaagatgtta tactataaga tatgcaggaa tgactcatgc ggcgcatgaa aaaaaagaac
     1621 gaagaggata catgtatatg gaaaacgaat atgttattga gatgttgaac atcacgaaag
     1681 aatttccagg aatcaaagcc aacgataata ttaccctgca gttgagaaag ggagagattc
     1741 atgcactgct tggtgagaat ggtgcaggga agtctaccct tatgagtatt ctgtttggtc
     1801 tgtaccagcc gacgtccggt acgatcaaaa agaacgggca ggttgtacag atcaatacgc
     1861 cgaacgacgc gaatgatctg aacatcggaa tggttcatca gcatttcaag ctggtggagt
     1921 gtttctccgt gctggataat attatccttg gtgttgagcc atcgaaagga ttgttccttg
     1981 aaaaagatgg tgccagaaaa aaggtactgg agctgagtga gaggtacggg cttaaggtgg
     2041 agccggatgc gctgatttcg gatatcaccg taggtatgca gcagcgtgtt gagattctta
     2101 agatgctcta ccgtgataac gaggtgctga tctttgatga gccgacggca gtgctgacgc
     2161 cgcaggagat cgaggaactg atgaagatta tgaagaatct cgcgcacgag ggcaaatcca
     2221 ttctgttcat cacgcataag ctgaatgaga tcatggaggt ggcggaccgc tgtacgatct
     2281 taagaaaagg aaagtacatc ggaacggtcg acatcgcgga tacgacgaag gaagaacttt
     2341 ccaagatgat ggtgggcaga aatgtaagct ttaccgttga caaggacgag aaaaagccgg
     2401 gggatacggt gcttgaggta tcgcatctga cggtggcaag cagatcacat gagaacaatg
     2461 ccgtgcggga tgtctcgttt caggtgcgcc gtggtgagat cgtatgtatt gcgggtatcg
     2521 acggcaacgg acagacagag ttggtgcagg ggctgaccgg actggagaag atcggagggg
     2581 gcagcgtgaa atttctggga caggatatca caaacaccac gatccgggag cgttccaagc
     2641 atggtatgag ccatattccg gaagaccggc ataagcacgg actggtactg gactacacgc
     2701 tggagcagaa tctcgtgctg cagcgctact ggcagccgca gtttgaacgc gcgggcttta
     2761 tcaagtcggg cgaggtgcgc agatatgcgc agaagctgat cgagcagtat gatgtgcgaa
     2821 gcggtcaggg acctgtgacc attgtgcgga gtatgtccgg cggtaaccag cagaaggcga
     2881 ttattgcaag agagattgac aaagacccgg agcttcttgt ggcagtgcag ccgaccagag
     2941 gtcttgacgt aggcgcgatc gagtatatcc ataagcagct tgtggcacag cgcgacaaag
     3001 gaaaagccgt gctgttagtc tcgttggagc tggatgaagt tatggacgtg tccgaccgta
     3061 tcctggtgat gtacgagggt gagatcgtag gagaactgga tccgaagaaa gtgactgtgg
     3121 aggaacttgg act
//
LOCUS       DBMDQJ010000257         3111 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11318_length_3111_cov_1.880066, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000257 DBMDQJ010000000
VERSION     DBMDQJ010000257.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11318_length_3111_cov_1.880066"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1366)
                     /locus_tag="ACZO9R_06215"
     CDS             complement(<1..1366)
                     /locus_tag="ACZO9R_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HIR0912694.1"
                     /translation="MDEAHVYIAIDLKSFYASVECVERGLDPLTTNLVVADPERTEKT
                     ICLAVSPSLKAYGIPGRARLFEVVQKVKEVNAGHPKERLTYQIAPPRMALYMDYSTRI
                     YNLYLRYVAPEDIHVYSIDEVFMDVTHYLGTYHMTARELAAKMIQDVFAETGITATAG
                     IGTNLYLCKVALDIVAKHVKPDRNGVRIAKLDEMSYRRLLWNYRPLTDFWRVGRGYQK
                     KLEENGLYTMGDIARCSLGGAREFHNEELLYRLFGINAELLIDHAWGWEPVTMEQIKA
                     YRPQVNSISSGQVLHCPYDAKGARLVVQEMADALALDLVERRLVTDQLVLTVGYDIEN
                     ITNPALYADYRGEIVADRYGRKIPKHAHGTVNLKTRTSSSRKIMEAVMGLYDDIVNPK
                     LLVRRITLVGNRLVEEARAEAEEQYEQLELFTDTGGQEEEKKQEELRLKKERSLQEAM
                     LSVKK"
     gene            complement(1450..2859)
                     /locus_tag="ACZO9R_06220"
     CDS             complement(1450..2859)
                     /locus_tag="ACZO9R_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005600952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR0912695.1"
                     /translation="MERNGTVMLKIIDDFVWGVPLIVLILAVGIFLTIRLRGLQITKL
                     PLAVRHIIANEKDGKDGEVSSFGALCTALSATIGTGNIVGVATAVVAGGPGALFWMWI
                     AALFGTATKYTECLLAIKYRVVEADGHVVGGPFYYIENGMGRKFRWLGQIFAFFGVGV
                     GLLGIGTFTQINGITGAVKGFFDPETACSVHLFSNTYSWSVVISGLILTVCVALVIIG
                     GIKRISSVAQVVVPFMALVYVVTVLIILLTHLGEIPAALATIVQSAFGLRAAAGGTLG
                     AILVAMQKGIARGIFSNEAGLGSAPIAAATAKTDEPVAQGLVSMMGTVIDTLIICTMT
                     GLSIIITDTWNIGLDGVDVTTRAFQIGLPFPERVSSFLLMLCLVFFAFTTILGWNYYS
                     EKCLEYLVGNRPGVLKCYRWLYILAVLIGPFMTVSAVWTIADIFNGLMALPNLIALVA
                     LSGVVAAETKQYFAKQKNF"
BASE COUNT          755 a          949 c          666 g          741 t
ORIGIN      
        1 ttttcttcac ggaaagcatt gcctcctgca ggctgcgctc ctttttcagc cgcagttctt
       61 cctgtttttt ctcctcttcc tgtccgcctg tatctgtaaa aagctccagt tgttcgtatt
      121 gttcttccgc ctcggcgcgg gcttcttcca ccagccggtt gcccacaagc gtgattctcc
      181 gcacgagaag ctttggattc acaatgtcgt catacagccc catcaccgcc tccataatct
      241 tacgggaaga ggaggttctg gttttcaggt tgaccgtacc gtgcgcatgc ttgggaatct
      301 ttctcccgta gcggtccgcc acaatctctc cgcgatagtc tgcataaagt gccggatttg
      361 taatattttc aatgtcataa ccgacggtaa gcacaagctg atccgtcaca agacgccgtt
      421 ccaccagatc cagcgccagc gcatccgcca tctcctgcac caccagccgc gcgcccttcg
      481 cgtcgtaggg gcaatgcagt acctgccccg aacttatgct gttcacctgc ggtctgtacg
      541 ccttaatctg ttccatcgtc accggctccc agccccatgc atgatcgatg agcagctccg
      601 cattgatgcc gaacagccgg tataacaatt cctcgttgtg gaactctctg gctcctccca
      661 gcgagcagcg cgcaatgtct cccatcgtat acagaccgtt ctcctccagc ttcttctggt
      721 agccccgtcc tacccgccag aaatccgtca gcggacggta attccagagc agtctgcggt
      781 aactcatctc gtccagtttt gcaatgcgca caccattccg atccggcttt acatgttttg
      841 ccacaatatc gagcgccact ttgcacagat agagattggt tccgatcccg gcggttgccg
      901 tgatgcccgt ctcagcaaag acatcctgta tcattttcgc cgcaagttct ctcgctgtca
      961 tgtggtatgt ccccagataa tgcgtgacat ccataaacac ctcgtcgata gaatagacat
     1021 gaatatcctc cggcgccaca taacgcagat agagattgta aattctggtg ctgtaatcca
     1081 tgtagagcgc catgcgcggc ggtgcgatct gataggtcag ccgctctttc ggatgcccag
     1141 cattcacttc ttttactttc tgtacgacct caaagagcct cgctcttccc ggaatgccat
     1201 aggctttcag ggaaggtgat actgccagac agattgtctt ttccgtgcgc tcggggtctg
     1261 ccaccacaag gttggtcgta agcggatcca gcccacgttc cacgcactct accgatgcat
     1321 agaaagactt taaatcaatt gcaatataca cgtgtgcttc gtccatctgc gccctccttt
     1381 tttctctatt attatagcag aaaaaaggac agagtatcca acaacttact ctgtcctttg
     1441 gcaaaaccat caaaaattct tctgttttgc aaaatactgc ttcgtctcgg ctgccaccac
     1501 accgcttagc gccaccagtg caatcagatt cggcagcgcc atcagtccgt taaaaatatc
     1561 cgcaatcgtc cagactgccg acacggtcat aaacggtccg atcaataccg ccagaatata
     1621 cagccagcga tagcatttta acaccccggg acggttgccc accagatatt ccagacattt
     1681 ctcgctgtaa taattccagc caaggatcgt tgtaaatgca aaaaacacca gacacagcat
     1741 cagtaaaaac gaggagacgc gttccgggaa cggaagcccg atctgaaacg ctcttgtcgt
     1801 gacatccacc ccgtccagac cgatgttcca cgtatccgtg atgatgatgg acaatccggt
     1861 catcgtacag ataatcagcg tatcgatcac cgttcccatc atggatacca gtccctgcgc
     1921 caccggctca tcggtcttcg cggtagctgc agcgatcggc gcacttccaa gccccgcttc
     1981 gttggagaaa atacctctcg cgattccctt ctgcatcgca acaaggattg cgccaagtgt
     2041 tcctcctgcc gctgcacgga gtccaaacgc gctttgtacg atggtggcaa gtgctgccgg
     2101 aatctctccc aaatgcgtca gcagaatgat cagcacagtc accacataca caagcgccat
     2161 aaacggcaca accacctgtg ccacggagga aatccgctta atccctccga taataacgag
     2221 tgccacgcac acggtcagta tcagcccgga tatcacaacg ctccacgaat aggtgttgct
     2281 aaacagatgc accgaacatg ccgtctccgg atcaaaaaag ccctttaccg caccggtaat
     2341 tccgttgatc tgcgtaaagg ttccgatgcc gagcagaccg actcccacgc cgaagaatgc
     2401 gaagatctgt cccagccagc ggaattttct gcccataccg ttctcaatat agtaaaacgg
     2461 tccgccgacc acatgtccat ccgcttccac cacgcggtac ttgatcgcca aaagacattc
     2521 cgtatacttg gtcgccgtgc caaacagcgc cgcaatccac atccagaaca aggcgcccgg
     2581 cccgcctgcc acgaccgccg tcgccactcc cacgatatta ccggtaccga tcgttgccga
     2641 cagcgccgtg cagagtgcgc cgaaactcga cacctctccg tccttgccgt ctttttcgtt
     2701 tgcaataatg tgtctgaccg caagcggcag cttcgtgatc tgaagtcccc gcagacggat
     2761 cgtcagaaat atgccaaccg caaggatcag cacgatcagg ggaactcccc agacaaaatc
     2821 atcaatgatc tttagcataa ccgttccatt cctctccatg taatactgca ctgttgcaaa
     2881 gtagcatagc acgaatgtgc gcggtttggc aagggatttt ttcaaagctt tgagaaagat
     2941 cttcactgga aaatcaagaa atttatagaa taaaaagcaa ggaacatacc gcttaaagta
     3001 tattccctgc ttttcaatta aatacgatac tacttagaac ttaccagctt tgtgagcttc
     3061 ctcaacactt actgcaacag caactgtagc accaaccatc gggttgttac c
//
LOCUS       DBMDQJ010000258         3099 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11364_length_3099_cov_1.539026, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000258 DBMDQJ010000000
VERSION     DBMDQJ010000258.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11364_length_3099_cov_1.539026"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="ACZO9R_06225"
     CDS             <1..1101
                     /locus_tag="ACZO9R_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HIR0912696.1"
                     /translation="GLQITVFSKGKVLTTQHLGLGTMRMREQLAKKSRNLAQYELQIE
                     EMVVKEMEVFKAMYLENQEIEYLIVIGDYIAELVCRLEKNHNKNTVSIEKFVQYMEQL
                     GKKSLEEIAEELGLSNENDPLIVPYIIICKCMARGLGAKELWAPGVIVSDGIACDYAQ
                     KNRIYKPAHDFDQDIISAAESLSRRYMSYSPHIEALSQMAMLLFDTMKKQHGLGKRER
                     LLLQVAAILHDCGKYISFANAPQCAYDIIMSSEIMGLSHLEREIVAGTVLYNSYPLAD
                     YEDVADRLDQQSFLTVAKLSAILRVSNAMDRSHKQKFKNVKAQLRDKELIITIETDED
                     IALEKALFASKTAYFERIFSIKPVVKEKRVYS"
     gene            1121..>3099
                     /locus_tag="ACZO9R_06230"
     CDS             1121..>3099
                     /locus_tag="ACZO9R_06230"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HIR0912697.1"
                     /translation="MEKEKKNEFHLPEYYENRELSWLKFDARVLNEAKDKSIPLLERL
                     KFVSITSSNLDEFFMVRVASLKDMVHADYRKRDIAGMTASEQLDRINTATRKLVESQY
                     NTYNRSLVPLMAANGIHIIEKYEELTAEQAAYVDRYFEEDVYPVLTPMAVDASRPFPL
                     IRNKTLNIAALLSSKKDEKHKDAVEFATVQVPGVLPRLVPIPADTSENSGEVEGRTFI
                     LLEQIIEKNIDKLFLNYHVLCAHPYRVMRNADLPIDEDEAADLLKEIQKQLKKRQWGE
                     VIRLEVEASVDKKLLRFLKDELKVAEEDIFQISGPIDLTFLMKMYGLSGCDSLRYEPY
                     KPQRVPEIEPGEDIFEAIRGGDILLHHPYETFDPVVDFIRQAASDPDVLAIKQTLYRV
                     SGNSPIIASLAQAAENGKQVSVLVELKARFDEENNIVWAKKLEQAGCHVIYGLVGLKT
                     HSKIALVVRREEDGIRRYVHLGTGNYNDSTAKLYTDCGIFTCNEAIGEDATAVFNMLS
                     GYSEPLSWNELVLAPIWLRTRFMRLIARETKHAREGKPAKIVAKMNSLCDEGIIAALY
                     EASAAGVKIELIVRGICCLKVGIPGISENIHVRSIVGNFLEHSRIFFFLNDGEEELYM
                     GSADWMPRNLDRRVEILFPVLDDTLKEKVKHILDV"
BASE COUNT          794 a          721 c          921 g          663 t
ORIGIN      
        1 ggacttcaga ttaccgtgtt ctcaaaggga aaggtgctga ccacccagca tctggggctc
       61 ggcaccatgc gcatgcggga acagcttgca aaaaagagcc ggaacctggc gcagtatgaa
      121 ctgcagatcg aggagatggt ggtaaaggag atggaagttt tcaaggcgat gtatctggaa
      181 aaccaggaga tcgaatatct gattgtgatc ggcgattata ttgcggaact ggtctgccgc
      241 cttgagaaga accacaacaa gaataccgtg tcaattgaga agttcgtcca atacatggaa
      301 cagctcggca aaaagagtct ggaggagatc gcggaggaac tggggctctc caacgagaac
      361 gatccgctga tcgtgcctta tatcatcatc tgcaagtgca tggcaagagg gctgggcgca
      421 aaggaactgt gggcgccggg agtgatcgtc agcgacggaa tcgcctgcga ctacgcgcag
      481 aagaaccgta tctataagcc tgcgcatgat tttgaccagg atattatttc ggctgcggaa
      541 agtctctcga gacgttacat gagttattcg ccgcacatcg aagcgttaag ccagatggcg
      601 atgcttctgt tcgacacgat gaaaaaacaa cacggtctcg gaaagaggga acggctgttg
      661 ctgcaggtcg cggcgatttt gcacgactgc ggaaaatata tcagctttgc caatgccccg
      721 cagtgcgcat acgacatcat catgtcctcg gaaattatgg gactgtcgca tctggagcgt
      781 gagatcgttg cggggacagt gctctacaac agctatccgc ttgccgatta tgaggatgtg
      841 gcggaccggc tcgaccagca gagttttctt acggtggcaa agttatccgc cattctgcgc
      901 gtttccaatg cgatggatcg cagccacaaa cagaagttta aaaacgtaaa agcacagctt
      961 cgcgacaaag aactgatcat tacgatcgag acggatgagg acattgcgct ggagaaggcg
     1021 ctgttcgctt ccaagacggc ttattttgag cgcattttca gcatcaagcc ggtcgtgaag
     1081 gaaaaacgtg tctattcgta aggacgtgga gggatgatac atggaaaaag aaaagaaaaa
     1141 cgaattccat ttgccggaat attacgagaa cagagagcta agctggctga agtttgacgc
     1201 ccgcgtgctc aacgaggcaa aggataagag cattccgctg ttagaacggc tcaaattcgt
     1261 gagcatcacg tcgtcgaacc tggacgaatt cttcatggtc cgggtcgcgt cgttaaagga
     1321 tatggtacat gcggactacc gcaagcggga tattgcgggc atgacggcat cggagcagct
     1381 cgaccggatc aacacggcga ccagaaaact cgtcgagagc cagtacaaca cgtataaccg
     1441 ctccctcgtt ccgctgatgg cagccaacgg aatacacatt attgaaaaat acgaggaact
     1501 gacggctgag caggcggcgt acgtggaccg ctactttgag gaggacgtct atccggtatt
     1561 gacgccgatg gcggtggacg catcgcgccc gttcccgctg atccgcaata agacgctcaa
     1621 catcgcggcg ctgctttcca gcaagaagga tgaaaagcat aaggacgcgg tggaatttgc
     1681 aacggtgcag gtgcctgggg tactgcctcg cctggtgccg atcccggcag atacgtcgga
     1741 gaattccggg gaggtggagg gacgcacctt tattctcctg gagcagatta tcgagaaaaa
     1801 tattgataag ctgtttttaa actaccatgt gctctgcgca catccgtacc gcgtcatgcg
     1861 taacgcggat ctgccgatcg atgaggacga ggcggcagac cttttaaaag agatccagaa
     1921 acagttaaaa aagagacagt ggggagaagt catccgtctg gaagtggaag cttccgtgga
     1981 caagaagctg ctgcgctttt taaaggatga actgaaagtg gcggaggagg atatttttca
     2041 gatatccgga ccgattgacc tgacatttct gatgaagatg tacgggctct ccgggtgcga
     2101 ttccctgcgc tacgaaccgt ataagccgca gcgcgtgccg gagattgagc cgggggagga
     2161 tatctttgag gcaatccggg gcggcgacat tctgctgcat catccgtatg agacgtttga
     2221 tcctgtggtc gattttatcc ggcaggcggc gtcggatccg gacgttcttg ccatcaagca
     2281 gacgctctac cgtgtcagcg gcaattcacc gattatcgca tctctcgcgc aggcggcgga
     2341 gaacggaaaa caggtgtcgg tgctcgtgga gctgaaagca cggtttgacg aggaaaataa
     2401 cattgtgtgg gcgaagaagt tagagcaggc gggctgccat gtcatctacg gtctggtcgg
     2461 tcttaagacg cacagcaaga ttgcgcttgt tgtgcgccgc gaggaagacg gcatcaggcg
     2521 ctatgtgcat cttggaaccg gcaattacaa cgattccacg gcaaagctct acacggactg
     2581 cggaatcttt acctgcaatg aggcgatcgg ggaggatgcg accgcggtgt ttaatatgct
     2641 ttccggatat tctgagccgc tttcctggaa cgaactggtg ctggcgccga tctggctgcg
     2701 cacccgtttt atgcgcctga ttgcacgtga gacaaagcat gcgcgcgagg gaaagccggc
     2761 gaaaatcgtt gccaagatga attccctgtg tgacgagggc attattgccg cgctctacga
     2821 ggcgtcggcg gccggcgtaa agatcgaact gatcgtccgc ggaatctgct gcttaaaggt
     2881 cggaatcccg ggaatcagcg agaatatcca tgtgcgttcc atcgtcggga atttcctgga
     2941 gcacagccgt attttcttct tcttaaatga cggggaggag gaactctata tgggaagcgc
     3001 ggactggatg ccgcgaaatc tggaccgtcg tgtggaaatt ttattcccgg tgctggacga
     3061 tacgttaaaa gaaaaggtaa agcatattct ggacgtgga
//
LOCUS       DBMDQJ010000259         3092 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11398_length_3092_cov_1.425610, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000259 DBMDQJ010000000
VERSION     DBMDQJ010000259.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3092)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3092)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3092
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11398_length_3092_cov_1.425610"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="ACZO9R_06235"
     CDS             <1..616
                     /locus_tag="ACZO9R_06235"
                     /inference="COORDINATES: protein motif:HMM:NF027442.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4832 domain-containing protein"
                     /protein_id="HIR0912698.1"
                     /translation="CAADEQAYLSGQDENILCGGEALAGIKLSAETVLEELQKMQVTY
                     LNSIYDPARMAEWKACRLPSGKSLYEEIGSRLGYRICVLCAEWRKGSLWVTLKNEGFS
                     AVCQKTDLLLIRECDEGKEQKVQKVPYEICGLKGGQTDVAKVEIAAGEDDGEGKEEKL
                     YLSMVRKKDGKPLPFANEGAGSRLLIGRWMVQGGLHGREKGNTD"
     gene            588..1670
                     /locus_tag="ACZO9R_06240"
     CDS             588..1670
                     /locus_tag="ACZO9R_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stealth CR1 domain-containing protein"
                     /protein_id="HIR0912699.1"
                     /translation="MEEKKETQIDFVIAWVDGGDPAWQRERDRYGKMGEKTAVEETGE
                     REKSGVACDTRDVRYRDWDCLRFWFRSVEQNAPWVHKIYFVTWGHVPKWLNGNHPKLE
                     IVRHEDYIPGEFLPTFNSHTIELNFHRIPGLSEQFVYFNDDMFLLQQVSPGQFFRDGK
                     PLDMLALQPVVANREDAVMPYIYLNNAMVLARYFDKRENMKQQPGAYWYPGYPLLYFG
                     YNLLERMFPRFTGFYTVHGPSPLLKSTYEALWQREGALLSEVCSHRFRSREDVSQYLL
                     REWQKLEGNFYPVNACRFCRYYDLSDQNTSLLHVIEKKKSPAVCINDSQKVEAYDRVK
                     RELNEAFLKAFPKKSSFETDGAGEKH"
     gene            1688..>3092
                     /locus_tag="ACZO9R_06245"
     CDS             1688..>3092
                     /locus_tag="ACZO9R_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR0912700.1"
                     /translation="METKSRTEYSAMNTTVAVISRMTAILMGFVTRVIFTHTLSENYV
                     GINGLFTDILNVLSLTELGAGTAITYALYRPIAEGNIKKQQQLMKLYQTFYRGTAVCV
                     GVLGLALIPFMDVLMKNRPEVEHLTFIYLLYLANSVLSYLLVYQRTLIEAHQMNYVVL
                     IYQTGFLVLQDLLQIVVLVTTGNFILFLLMYLMCTVLSNVCISRKAEKLFPYLKEKEK
                     ERLPAEERKGLFRNIRAMMMHKLGTVVVNNTDNLLISAFVGVVSVGIYSNYYLLIGSV
                     RQVLDQVFAGITASVGNLGATEDQGKIREIFETAFFIGQWLYGFAAICLYELLNPFVE
                     LAFGRQYLFERSVVMILCINFFINGTRKAVLTFRDSMGLFWFDRYKALAEAALNLVLS
                     ILLVRQYQTLGVFIGTFLSTILTSVWVEPFVLYRRRLHLPVHRFYLRYLLYAVCVGAV
                     WIVTDLVCGLAEGSPQTR"
BASE COUNT          737 a          644 c          952 g          759 t
ORIGIN      
        1 gtgtgcggca gatgagcagg cttatctgag cggacaggat gaaaatattc tgtgcggagg
       61 ggaggcgctg gcgggaataa agttgtctgc agagacggtc ttagaggaac ttcaaaagat
      121 gcaggtgacc tatttaaaca gcatctatga cccggcgcgg atggcagagt ggaaggcatg
      181 ccgtctcccg tccggaaaat cgctgtatga ggagatcggc agccgccttg gttaccggat
      241 ctgtgtgctt tgtgcagagt ggagaaaggg aagcctttgg gtgacactga aaaatgaagg
      301 attttccgcc gtatgccaga aaacggatct gcttctgatc cgggagtgtg atgaaggcaa
      361 agagcagaag gtgcagaagg ttccttatga gatctgcgga ttaaagggcg gtcagacgga
      421 cgtggcaaag gttgaaattg cggcgggaga agacgacggg gaaggaaaag aggagaaact
      481 ctacctttcc atggtgcgga agaaagacgg aaaaccgctg ccgtttgcga atgaaggcgc
      541 gggaagccgg ctcttgatcg gcagatggat ggtgcaggga ggattgcatg gaagagaaaa
      601 aggaaacaca gattgatttt gtgattgcct gggttgacgg cggggatccg gcatggcaga
      661 gagagcggga tcgatatggg aagatggggg agaaaacagc ggtagaagag accggagaga
      721 gagagaagtc cggcgttgcc tgcgatacca gggatgtgcg ctaccgtgat tgggactgcc
      781 tgcggttctg gttccgcagt gtggaacaga atgccccgtg ggtgcacaag atctattttg
      841 tcacatgggg acatgtgccg aagtggctga atgggaatca tccgaagctt gagattgtgc
      901 gccacgagga ttatattccg ggagaatttc tgccgacgtt taattcccac acaatcgagc
      961 tcaattttca ccgcatcccc ggattgtcgg aacagtttgt ctattttaac gacgatatgt
     1021 ttctgctgca gcaggtaagc cccggacagt ttttccggga cggaaagccg cttgacatgc
     1081 ttgccttgca gccggtcgtg gcaaaccggg aggatgccgt catgccgtat atttatctga
     1141 acaatgcgat ggtgcttgcg aggtattttg acaagcggga aaacatgaag cagcagccgg
     1201 gagcctactg gtatccgggg tatccgctct tatatttcgg gtataacctg ctcgagcgca
     1261 tgttcccgcg gtttaccgga ttttatacgg tgcacggtcc gtcgccgctt ctaaagagca
     1321 cctacgaggc gctctggcag cgggagggag cgcttttgtc ggaggtgtgc agccacagat
     1381 tccgcagcag ggaggatgtg agtcagtatc tgctcagaga atggcagaag ctggagggta
     1441 atttttatcc tgtcaatgcc tgccggttct gcaggtatta tgatctctcg gatcagaata
     1501 catcgctgct tcatgtgatt gagaagaaaa aaagcccggc tgtctgcatc aatgattccc
     1561 agaaggtgga ggcgtatgac agggtcaaga gggaattaaa cgaagcattt ttaaaagctt
     1621 ttccgaaaaa gagttccttt gaaacggacg gagcagggga aaagcactaa gacagagagg
     1681 agacgccatg gaaacaaaga gcagaacaga atattcggca atgaatacaa ccgtggcggt
     1741 catctccaga atgacggcga ttctgatggg atttgtgacg cgtgtgattt ttacgcatac
     1801 gctgagtgaa aactatgtcg gaatcaacgg tctgttcacc gatattttaa acgtgctttc
     1861 gttgaccgag cttggcgcag gcacggcaat cacttacgcc ttgtaccggc cgatcgcgga
     1921 gggcaacatc aagaagcagc agcagctgat gaagctttac cagacatttt accgtggaac
     1981 cgcggtgtgt gtgggcgtgc tgggactggc gctgattccg tttatggacg tgctcatgaa
     2041 aaacagaccc gaggtggagc atctgacgtt tatttacctt ctctatctcg ccaactccgt
     2101 gctgtcgtat ctgctggtgt accagagaac gctgattgag gcgcatcaga tgaattatgt
     2161 ggtgctcatc tatcagaccg gctttttggt gctgcaggat ctgctgcaga ttgttgtgct
     2221 ggttactacg ggaaacttta ttctgttttt gttgatgtat ctgatgtgca cggttctgtc
     2281 aaatgtgtgt atctcgcgaa aggcggaaaa gcttttcccg tatttaaagg aaaaagagaa
     2341 ggaacgtctt cccgcagagg agcggaaggg actgtttcgg aatatccgtg ccatgatgat
     2401 gcacaagctt gggacggtcg ttgtaaataa taccgataat ttactgatct ccgcgtttgt
     2461 cggcgtggtc agtgtcggaa tatattccaa ttattatctt ctgatcggtt ccgtccgcca
     2521 ggtgttggac caggtctttg cgggaattac agcgagtgtg ggcaatctgg gagcaacgga
     2581 ggaccagggc aaaatccgcg agatttttga gacggccttt tttatcgggc agtggcttta
     2641 cgggtttgcg gcgatctgcc tgtacgaatt gctcaatccg tttgtggagc tggcttttgg
     2701 cagacagtat ctgtttgaac gctccgttgt catgatctta tgcatcaact ttttcatcaa
     2761 cggaaccagg aaagcggtgc tgacctttcg ggattccatg ggattgttct ggttcgaccg
     2821 ctacaaggcg ctggcggagg cggcgctcaa tctggtgctg tcgattcttc tggtaagaca
     2881 gtatcagacg ctcggcgtat ttatcggaac gtttttgtca acgattctca cgtcggtgtg
     2941 ggtggagccg tttgtcctgt accggagaag attgcacctg ccggtgcacc ggttttatct
     3001 gcgctacctt ttgtacgcgg tctgtgtggg agcggtatgg attgtgacag acctggtctg
     3061 cggactggca gagggaagtc cgcagaccag gt
//
LOCUS       DBMDQJ010000260         3066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11509_length_3066_cov_1.754132, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000260 DBMDQJ010000000
VERSION     DBMDQJ010000260.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11509_length_3066_cov_1.754132"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="ACZO9R_06250"
     CDS             complement(<1..740)
                     /locus_tag="ACZO9R_06250"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0912701.1"
                     /translation="MEKIRHDSSYEWLSGESWKEWSASFFSMGWKIWGVVLITELFLF
                     LFFKPTADCGRLRYGWLFIVRPSGLQLLLLFVYRLLTGRQKRVYSRRAMAGCAIFLLT
                     SYAAVMAWVHTSVTLMPMVLLLPMLLTPLYKDRVMTLVQAALLVAAYVLDCVYFIPAS
                     SYFPLTTPFIEGSIFVGTVCGMLVALELVNDSAIVNDERSKRDSLTKLYNHESFYEEL
                     EYYQRRYEENRECFSLAVIDIDNFKNVN"
     gene            complement(897..1475)
                     /locus_tag="ACZO9R_06255"
     CDS             complement(897..1475)
                     /locus_tag="ACZO9R_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071421 - manganese ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HIR0912702.1"
                     /translation="MNWIENVLIIAGVSLDIFAAMECQGALVAKVDKKQLSVICALIA
                     VWQLCALALGNYLSGLLYRNELAHDENFVGLVIAAVIFFGLGIRLIAKAIKNEWVNER
                     REETLDVKKFLRMTAVTSIYTLLAGIAFGFLGTNVALMLIIIVCMTIAFVVLGVYTGY
                     HLGFQHKTKAYVGGALLLWIAGIDVIVRHIMC"
     gene            complement(1565..>3066)
                     /locus_tag="ACZO9R_06260"
     CDS             complement(1565..>3066)
                     /locus_tag="ACZO9R_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912703.1"
                     /translation="VKSKMGDFKDGVGTLQSGVHAYTDGAGKIASGITQLSDSIPTLS
                     NGVGTLNSSAATIADGVELLDNTLKTSFTDQEKAAMQTQASSAVDAQAEAIKNRASAA
                     VDARAEAIKNQASSTVDAQAEAIRSQASAVVDAQAEAIKTQAEAVVDGQAEAIKNQAA
                     QQAGAKFDNGQYEAIKNQATQEITTKMDGLKGSLTSSADVQNLINGAALASVAAQYPG
                     ENIADIPSNHPDKYAEAQQTVAAQLDTTITTQLGGAADSLATGVADTAKQVAQATAGE
                     AAVTGAKTAAGDAAVLGAKAAAGDAAVLGAKAAAGDAAVLGAKAAAGDAAVLGAKEAA
                     GTAAVSAAEQTKSTMAASIEQQSSGGYSLVTGARALANGTQSLADAVPALTSGVAQLK
                     NGSAELVSNNQKLNDGVNALSDGTDAVIDGVDQLSDGAHQLADGIVIFNEEGIEKILN
                     SYHGDIEPLMDRIQAVLDAGEDYQTYTDIADGVNGSIKFIYKTDAVKED"
BASE COUNT          672 a          956 c          650 g          788 t
ORIGIN      
        1 tcattgacat tcttaaaatt atcaatatcg atcaccgcaa gcgaaaaaca ctcccggttc
       61 tcctcgtacc ttctctggta atactctaac tcctcataaa agctttcatg gttgtagagc
      121 ttggtcaggg aatcccgctt ggaacgctca tcgttcacga tcgccgaatc gttgaccagt
      181 tccagcgcca ccagcatacc gcacaccgtt cccacgaaga tgctgccctc aataaacggg
      241 gttgtcagcg ggaaataaga actggcaggt ataaaataga cacagtccag cacataggct
      301 gcaacgagca gtgccgcctg cacaagcgtc atgacccggt ctttgtagag cggcgtcagc
      361 aacatcggca gcaacagaac catcggcatc agcgtcacgc tcgtatgcac ccacgccatc
      421 acagccgcgt aggatgtcag caggaatatg gcgcatcccg ccatcgcccg tctgctgtat
      481 acacgcttct gtctgccggt cagcaggcgg tacacaaaaa gaagcaacag ctgcagtccg
      541 ctcggtcgca caatgaacag ccatccatag cgcagtctgc cgcagtccgc ggtgggctta
      601 aaaaacagaa acaaaaacaa ttctgtgatt aacaccacgc cccagatctt ccagcccatc
      661 gaaaagaagc tcgcgctcca ctccttccag ctttcgccac tcagccattc atagctgctg
      721 tcatgtctga tcttctccat catttcccct cattacgaaa cgtaatactt tcttcctaga
      781 atattatact gtatcacacc gattctttca acggcaagta aacgctctcc gatcagaaaa
      841 aaccagagac agctccccaa ccgtctctgg ttctatttga aatactctcc ctctttttag
      901 cacatgatat gtctgacaat cacatcgatg ccggcaatcc acagcagaag cgctccgccg
      961 acatatgctt ttgtcttgtg ctgaaatcct agatgataac ccgtatacac gccaagcaca
     1021 acgaatgcaa tcgtcataca tacgatgatg atcagcatca gcgcaacgtt cgtccccagg
     1081 aatccgaatg cgatccccgc aagcagcgta tagatgctcg tcaccgcagt catccgcagg
     1141 aactttttca catcaagggt ctcctcccgg cgctcattga cccattcgtt cttgattgcc
     1201 ttcgcaatca gacggatgcc gaggccgaaa aagatcaccg ccgcaatcac aagtcctaca
     1261 aaattctcat cgtgtgccaa ctcatttctg taaaggagac cggacagata gttcccgagc
     1321 gccagcgcgc agagctgcca tacagcgatc aatgcgcaga tgacggacaa ctgttttttg
     1381 tccacttttg cgaccaatgc tccctggcac tccatcgccg cgaaaatatc aagcgatact
     1441 cccgctataa tcagaacatt ctctatccag ttcatttccg tcctccctta catgactcct
     1501 tattattata ctctcttctt acaatatttc cagaacaaat acggatttat tgagacaatt
     1561 ttcattagtc ctctttcacc gcatctgtct tgtagatgaa cttaatgctt ccgttcacgc
     1621 cgtccgcaat atccgtgtag gtctgatagt cctcaccggc atccagaacc gcctggatac
     1681 gatccatcag aggctcaata tcaccgtggt aggaatttaa gatcttctca attccctcct
     1741 cgttgaagat aacgattccg tctgccagct gatgtgcacc gtccgataac tgatccacac
     1801 cgtcgatcac tgcgtcggta ccatcactta acgcgttgac gccatcgttg agcttctgat
     1861 tgttggaaac cagttctgca cttccgttct taagctgcgc cacaccggat gtcagtgccg
     1921 gtaccgcatc tgccagactc tgggtaccgt tagccagtgc tctcgctccg gttaccagac
     1981 tgtatccgcc cgagctctgc tgctcaatac ttgccgccat cgtggacttt gtctgttctg
     2041 ccgctgacac agctgctgtt ccggctgctt ccttcgctcc aagtaccgct gcatctccgg
     2101 ccgctgcctt tgctccgagt accgctgcat ctccggctgc tgccttcgct ccgagtaccg
     2161 ctgcatctcc ggctgccgcc ttcgctccga gtaccgctgc atctccggct gctgtctttg
     2221 ctcccgttac tgctgcctcg cctgccgttg cctgagcgac ctgtttcgca gtatctgcca
     2281 cccccgtcgc cagagaatct gccgctccgc caagctgcgt tgtgattgtg gtatcaagct
     2341 gtgctgcaac cgtctgctgt gcctctgcat atttatccgg atgattgctc ggaatatctg
     2401 caatgttctc acccggatac tgtgctgcaa ccgatgcaag tgccgctccg ttaatcagat
     2461 tctgcacatc cgcagaagat gtcagagatc ccttcagacc gtccatcttc gtggtaatct
     2521 cctgcgttgc ctgattcttg atggcttcat actgtccgtt atcaaacttt gcgcctgcct
     2581 gctgtgcagc ctgattctta atcgcttccg cctgaccgtc cactactgcc tctgcctgtg
     2641 tcttaatcgc ttctgcctgc gcatctacca ctgcagatgc ctgactcctg atcgcttctg
     2701 cctgtgcatc taccgtggaa gatgcctgat ttttaatcgc ttctgcccgc gcatctaccg
     2761 ctgcggatgc ccggttcttg atcgcctctg cctgtgcatc cactgcggac gatgcctgtg
     2821 tctgcatcgc tgccttctcc tgatcggtaa aggaagtctt taaggtattg tctaacagtt
     2881 ctacgccgtc cgcaatcgtt gccgcactgc tgttcaatgt gccgacaccg ttagataatg
     2941 tcggaatgct gtcggaaagc tgcgtgatac cgcttgcgat tttccccgct ccgtccgtgt
     3001 atgcatgaac gccggactgc agggtgccaa cgccgtcctt aaagtcgccc atcttggact
     3061 tcacgg
//
LOCUS       DBMDQJ010000261         3066 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11510_length_3066_cov_1.496459, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000261 DBMDQJ010000000
VERSION     DBMDQJ010000261.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11510_length_3066_cov_1.496459"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            148..1545
                     /locus_tag="ACZO9R_06265"
     CDS             148..1545
                     /locus_tag="ACZO9R_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF342 domain-containing protein"
                     /protein_id="HIR0912704.1"
                     /translation="MAELTQQKPIIRITFDEMEAYMLLPEPEQGTGYTDSQIRQEMAA
                     RGITTGIDEQRISDMLEGHTYNAELLVAQGKKPVDGTDGYYEYKFDTNFDGKPKLLPD
                     GSVDYWSVHSIESVTAGQVIAVYHPAVSGEDGMSVKGRLVPAKHGREQMPLKGKGFDR
                     MDDEVTYTASMDGKIEMQNDRIVVLPVHEVSGNAELAEGNIDFRGDIVIHGNVESGVS
                     IRATGSITIDGVAEACMLEAGKDIILRGGMLGGNKASVKTKGAITAKFFEFTNILCEG
                     DLQADVLMDCSVICHGQIRLTGRKGSIIGGKVQAIRGLIATSLGNDAEKRTEIFVGAG
                     IDVYSRLRVMEKKIEMTREELAKIEEGLEKFEILSKERGVSYTNDPRRTSLLRIKIKD
                     SAMLASDEEEVRKLRILAESSKGACVSVLQEIYPGVIINMDELKLALKNMATGVEFYK
                     LPDKIGTRPCSAGVE"
     gene            1634..1795
                     /locus_tag="ACZO9R_06270"
     CDS             1634..1795
                     /locus_tag="ACZO9R_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912705.1"
                     /translation="MTEVITVINFEEELKNFKPSLEVEEAEQAIYNHELTDMTDVLQE
                     ILQDMKRGM"
     gene            1834..>3066
                     /locus_tag="ACZO9R_06275"
     CDS             1834..>3066
                     /locus_tag="ACZO9R_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0912706.1"
                     /translation="MECYNCGAKLGKGDICQNCGANVKIYKKIIMASNAYYNDALEKA
                     AVRDLSGAIESLRTSLRFYKMNIDARNLLGLVYFETGEVVEALTEWVISKNYQPRDNA
                     ASRYLNEIQNNPSRLDTINQTIKKYNQALLYCRQDSRDLAIIQLKKVLSLNPKLVSGH
                     QLLALLYIQEKKYDLAKKSLRNAGKIDANNTTTLRYLKEVNAGLRENNPKKQKNEDLI
                     SYQSGNETIIQPRYLKDNSAIGTIINMVIGIAIGAAITCFLIVPGVRREIQNQAKAEV
                     LDANNAVASKNQSISSLEAQIEDLTSQITDAKNEEESSAGKVNTYEQLLQAYVAYTDG
                     DIEKAGTALGNVNTDYLSDDSMTVYDTINAQVNAEYISAVYKEATDAYNAQEFTQAIE
                     DYLKVIELDETYDNGNALY"
BASE COUNT          870 a          648 c          913 g          635 t
ORIGIN      
        1 cgctagaaaa gagttagaga aaccctgcgt attgtaccgg aaacgcaaaa agatgtactt
       61 gaaaaagtac catcggacta gtatgataga atgtagatgg attgacagta gcaggtggga
      121 tttacacaga ttggggagcg gcagactatg gcggaactta cacaacagaa acctattata
      181 cgcataacat ttgatgagat ggaggcctat atgctgctcc cggagccgga acagggaaca
      241 gggtatacgg actcccagat ccggcaggag atggcagcga gaggaatcac cacaggcata
      301 gatgagcagc ggatttccga tatgctggag gggcatacat acaatgcaga gcttctggtc
      361 gcgcagggga agaagccggt ggatggaacg gacggttact acgaatacaa gttcgacacg
      421 aactttgacg gcaagccgaa gcttctgccg gacggttctg tggattactg gtcggtacat
      481 tcgatcgagt ctgtgactgc cggtcaggtg attgccgtgt atcaccctgc tgtgtctggc
      541 gaggatggaa tgtcggtcaa gggcaggctg gtgcctgcca agcacggaag agaacagatg
      601 ccgttaaagg gcaaaggttt cgatcgtatg gacgatgagg tgacttatac ggcttccatg
      661 gacggtaaga tcgagatgca gaatgaccgg attgtggttc ttccggtaca tgaagtctcg
      721 gggaatgcag agctggcaga gggtaatatc gacttccgcg gagatattgt cattcacggg
      781 aatgtggaga gcggcgtcag catccgggca accggatcaa tcacgatcga cggtgtcgcc
      841 gaagcgtgca tgctggaagc cggcaaggac atcatcctaa gaggcggtat gctcggcggc
      901 aacaaggcgt ctgtgaagac gaagggcgcg attactgcga agttttttga gttcacgaac
      961 attctgtgtg agggtgatct gcaggcggat gtgctgatgg attgttcggt gatctgccat
     1021 gggcagatac gtctgaccgg aagaaagggc agtattatcg gcggtaaggt acaggcgatc
     1081 cgcggactga ttgcaacctc gcttggcaat gatgccgaga agcgcaccga gatctttgtc
     1141 ggtgccggaa ttgatgtgta cagccgcctg cgtgtcatgg agaagaagat cgagatgacg
     1201 agggaggaac tggcgaagat cgaggaagga ttggagaagt tcgagattct ctccaaggag
     1261 cgtggcgtca gctacacgaa cgatccgcgc cggacgtcgc tgcttcggat taagatcaag
     1321 gattctgcaa tgcttgccag cgacgaggag gaagtgcgga agctccgtat tcttgcagag
     1381 agttcaaaag gggcttgtgt ctcggtgctc caggagattt atcccggtgt gatcatcaat
     1441 atggacgagt tgaagcttgc cttgaaaaat atggcaaccg gagtggaatt ttacaaatta
     1501 ccggacaaga tcggaacaag accgtgcagc gccggtgtcg aatagcagaa gtttctgcgt
     1561 caaaaatagc ttgattatta gcaccactat attataatga gaaagtgaat aatatagtgg
     1621 tgtttttata ttgatgacag aggtgataac agtgatcaat tttgaagaag aattaaagaa
     1681 ttttaagccg agcctggaag tggaagaggc ggagcaggct atctataatc atgaactcac
     1741 ggatatgacg gacgtattgc aggagatcct gcaggatatg aagcgcggca tgtagaagca
     1801 ggcagggtca ggattgataa agtgaggttt tccatggaat gttataattg cggggcgaag
     1861 ctgggcaagg gcgatatctg ccagaactgc ggagccaatg ttaaaatata caaaaagatc
     1921 attatggcgt ccaatgccta ctataatgat gcgttagaga aggcggcagt gcgggacctg
     1981 tccggcgcga tagagagtct taggaccagc cttcgttttt ataagatgaa tatcgatgcg
     2041 cgcaacctgc tcggactggt ctacttcgag acgggcgagg tggtcgaagc gttgacggag
     2101 tgggtcatca gcaagaatta ccagccgagg gacaacgccg caagccggta tttaaatgag
     2161 atccagaaca atccgtcaag gctggatacg atcaaccaga cgatcaaaaa atacaaccag
     2221 gcgctgctgt actgcagaca ggacagccgg gatctggcga tcatccagct caagaaagtg
     2281 ctgtccttaa atccgaagct ggtaagcggt catcagctgc tggcgctcct ctacattcag
     2341 gagaagaagt acgaccttgc caagaagtcg cttcggaatg cgggaaaaat tgacgccaac
     2401 aacaccacga cgctgcgcta tttaaaagaa gtcaacgccg gattgcgtga gaataacccg
     2461 aaaaagcaga agaacgagga tctgatctcc tatcagagcg gcaatgagac catcatccag
     2521 ccgcgctatc tcaaggacaa ttccgcaatc ggaacgatca tcaatatggt gatcggaatt
     2581 gcaatcggtg cggcgatcac ctgctttctg attgtgccgg gagtgcgcag ggagatccag
     2641 aatcaggcga aagcggaggt tcttgacgcc aacaatgccg tggcatccaa gaatcagagc
     2701 atcagttcct tagaagcgca gatcgaagat ctcacatcac agatcacgga tgcgaagaac
     2761 gaggaggaga gcagcgccgg aaaggtcaac acttacgagc agcttctgca ggcgtatgtg
     2821 gcgtacaccg acggtgacat cgagaaggcg gggacggcac tcggcaacgt caataccgat
     2881 tatctttcgg atgactccat gactgtgtac gatacgatca atgcacaggt aaacgcggag
     2941 tatatcagcg ccgtatataa ggaagcaaca gatgcttaca atgcgcagga gtttacacag
     3001 gcaatcgagg attacctcaa ggtgatcgaa ctcgatgaaa cctacgacaa cggcaatgcg
     3061 ctgtac
//
LOCUS       DBMDQJ010000262         3065 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11512_length_3065_cov_1.474696, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000262 DBMDQJ010000000
VERSION     DBMDQJ010000262.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11512_length_3065_cov_1.474696"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="ACZO9R_06280"
     CDS             complement(<1..634)
                     /locus_tag="ACZO9R_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0912707.1"
                     /translation="MMRFKHLFSTVIVVLFAVVLAACSHHTPVESKKDSTGYTPDPAT
                     EYLVTQYADATGVQGTFYTITNDTTLIIIDGGWADNASAVREVIAAHGNHVNAWILSH
                     PHQDHAGAFNQIYASPDGITIDAVYDNGFDYDFIEAAGEPYDDITVMETYHTLTRDAS
                     NVTHLHRGDVLSICGLTFSVLNAYDDTVLQNVGDEKDYQNNASLLLKVSSV"
     gene            802..>3065
                     /locus_tag="ACZO9R_06285"
     CDS             802..>3065
                     /locus_tag="ACZO9R_06285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912708.1"
                     /translation="MDTHKFFVEKMRFHLKEQDTLVFVGWFFDGSTKEHSIKAYLDNR
                     ELPLTVQVNRGAEVRQKYIRSINEIEEEVVGIIKLPPDWRNGRRVTLMAAYREEYKKS
                     YAISTAKLGRLENEVSYYVENCHREGSQITVTGWCMSAGDIKLELLDGSRQPISEKVE
                     HYYRKDLLSVFPECDKEAKPGFLVQGTAAGGQSGPFYLEMRGAKNHSRTRLTKWDEGT
                     KLQYVIEKAGDTVRYLERNGISATAYKIKSKLTHKEGNTYENWRAKYLPSPEELQRQR
                     EDVFETGPLFSLVVPLYKTKPQYLREMIDSVTAQTYGNWELCMALAASGEEDAALISI
                     LEEYAGRDARIHYQVLPENGGIAENTNAALVMASGDYLVPVDHDDLVPENALYEFASA
                     IRRDDAIDMLYSDEDKVSMDGRHFFEPHFKPDFDLDLLCSVNYICHLLAVRKDVAERA
                     GSYQSAFDGAQDLDFILRCSEQAKKIYHVPKILYHWRCHMDSTASNPESKLYAFEAGR
                     RAIEEHYRRLGIPARVENASFYGMYRTVYEWKEEPLVSIIIPNKDHAEDLKLCLDSIF
                     TKSDYRNFEVVIVENNSTEPETFAYYKELEAGHENVRVVYYEGGFNYSKINNFGAAAC
                     RGDYFLLLNNDTEMIDGGCIREMLGYCMREDVGIVGAKLLYADDTIQHAGVILGFGGT
                     AGHAFIGKSRYDTGYFGRILCAQDYSAVTAACMMTKRQAFEAVGGMTGELAVAFNDVD
                     YCLKVRKHGWLVVY"
BASE COUNT          765 a          663 c          955 g          682 t
ORIGIN      
        1 ttacgctgct tacttttaag aggagggaag cattgttctg gtaatctttc tcatctccga
       61 cattctgcag aaccgtgtcg tcataggcat ttaggacgga gaacgtcaga ccgcagatgg
      121 agaggacatc gccgcggtgc aggtgcgtca cgttggaggc atcccgggtg agcgtgtggt
      181 aggtctccat caccgtaatg tcatcgtacg gctctccggc cgcttcgata aagtcatagt
      241 cgaaaccgtt gtcgtagacg gcgtcgatcg tgatgccgtc cggtgaggcg tagatctgat
      301 tgaatgcgcc ggcgtgatcc tgatgcggat gtgagagaat ccaggcgttg acatggttcc
      361 cgtgggcggc gatgacctcg cgtacggcgg aggcgttgtc tgcccagccc ccgtcgatga
      421 tgatgagggt ggtatcgttc gtgatggtgt aaaaagttcc ctgcacgccg gttgcgtccg
      481 cgtattgcgt caccagatat tccgtggcgg gatcgggggt gtagccggtg gaatcttttt
      541 tggattccac gggtgtatga tgggagcatg ccgcgagaac tacggcaaaa agaacgacaa
      601 tgacagtgga aaaaaggtgt ttaaaacgca tcataaggac tcctttgagt tgatcggttt
      661 attctttgca ttctacatta acagagaaca aaaggaatgt aaaggcgtgg taacaacttg
      721 acgcaaaacg tcggaattgt tatagtggaa tacgaaatca aagagattga ccgaggagtc
      781 aatggcgaga gaggacggac catggataca cataaatttt ttgtggaaaa gatgcggttc
      841 catctgaagg aacaggacac gctggtcttt gtcgggtggt tttttgatgg gagtacgaag
      901 gaacattcca tcaaggcata tctggacaac agagagcttc cgcttacggt acaggtgaat
      961 cgcggtgcgg aggtccggca gaaatatatc cgcagcatca atgagatcga ggaggaagtg
     1021 gtaggcatca taaagcttcc gccggactgg cgcaatggcc gccgggtgac attgatggct
     1081 gcttacaggg aagaatataa gaaatcttat gccatctcaa cagcaaagct tggcagactg
     1141 gagaatgaag tcagctacta tgtggaaaac tgtcacagag aggggagcca gattacggtc
     1201 actggctggt gcatgtcggc gggggatatc aagcttgaac ttctcgacgg cagcagacag
     1261 ccgatttccg agaaggtgga gcactattac cgcaaggatc ttctttcggt ttttccggaa
     1321 tgtgataagg aggcgaagcc cggttttctg gtgcagggaa ccgcagcggg agggcagagc
     1381 ggaccgtttt atctggagat gcgtggcgcg aagaatcatt cgagaaccag actcacgaag
     1441 tgggacgagg gcacgaagct gcagtatgta atcgaaaaag ccggggatac cgtccgctat
     1501 ctggagcgca acggaatctc tgcaaccgca tataagatta agagcaagct gacgcacaaa
     1561 gagggcaata cctatgagaa ctggcgggcg aagtatctcc cgtcgccgga agaactgcag
     1621 cgccagagag aagatgtgtt tgagacggga ccgctgtttt cgctggtcgt gccgctctat
     1681 aagacgaagc cgcagtatct gcgggagatg attgattccg tgacggcgca gacttacgga
     1741 aactgggaac tgtgtatggc gcttgccgca tccggggaag aggatgcagc actgatctcc
     1801 attctggagg agtatgccgg gcgtgatgcg cggattcact atcaggtgct gccggagaac
     1861 ggaggaatcg cggaaaatac gaacgccgcg cttgtaatgg cttccggaga ctatcttgta
     1921 ccggtggacc atgacgatct cgtgccggaa aatgcgctat acgaatttgc gtcggcgatc
     1981 cgcagggacg atgcaatcga catgctctat tccgacgagg acaaggtcag catggacggc
     2041 agacactttt ttgagccgca tttcaaaccg gactttgacc tggatctgtt gtgcagtgtg
     2101 aattacatct gccacctgct cgcagtgcga aaagatgtgg cggagcgtgc ggggagctac
     2161 cagagcgcgt ttgacggcgc gcaggatctg gactttatcc taagatgcag cgagcaggcc
     2221 aaaaagattt atcatgtgcc gaagattctg tatcactggc gctgccatat ggactcgacc
     2281 gcatccaatc cggagagcaa gctctatgcg tttgaggcgg gacggcgcgc cattgaggag
     2341 cattaccgca gactgggaat accggcgcgc gtggaaaacg catcgttcta cggaatgtac
     2401 cgcacggtct acgagtggaa agaggaacct ctggtatcca ttatcatacc aaacaaggat
     2461 cacgcggagg atttaaagct gtgtctggat tccattttta caaaatccga ctaccggaat
     2521 tttgaggttg tgattgtgga aaataacagt accgagccgg agacgtttgc ttattataag
     2581 gaacttgagg cggggcatga aaatgtccgg gtggtgtact acgagggtgg ctttaactac
     2641 tcaaagatca ataatttcgg cgcggcggca tgcaggggag attatttcct gctgctgaac
     2701 aacgatacgg agatgattga cgggggctgt atccgggaga tgctcggata ttgtatgaga
     2761 gaggacgtcg gaatcgtcgg tgcaaagctc ttgtatgcgg acgatacgat ccagcatgcc
     2821 ggtgtgatct taggctttgg cggcacggcg ggacatgctt tcattggaaa atccagatac
     2881 gatacgggat attttggcag aattctgtgc gcgcaggatt attcggcggt taccgcggcg
     2941 tgcatgatga cgaagcgcca ggcgtttgag gcggtcgggg gaatgaccgg ggagctcgcc
     3001 gtggcgttca atgatgtgga ctactgcctg aaagtaagaa agcacggatg gctcgtcgtg
     3061 tacga
//
LOCUS       DBMDQJ010000263         3061 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11524_length_3061_cov_1.498649, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000263 DBMDQJ010000000
VERSION     DBMDQJ010000263.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11524_length_3061_cov_1.498649"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="ACZO9R_06290"
     CDS             <1..773
                     /locus_tag="ACZO9R_06290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRM11 family SAM-dependent methyltransferase"
                     /protein_id="HIR0912709.1"
                     /translation="QAHRETTPFYFRLEIKSHMPLDKKSTFARRFSAELERLSGRMLV
                     NAPSDYEIELRLLEKRDGGYACFLKLLTIAHGRFAYRKNAVAASIHPATAAMLVALAE
                     PYLKENAQILDPFCGVGTMLIERDIRVPAREKYGIDIFGEAIRGARENASYAGEKINF
                     INRDYFDFTHAYLFDEIITNMPVRGRKSKEETDAFYSRFFEKSAGLLKADGILVLYSN
                     EQGFIRKQLRIRSDYRLLAEQCIREKDQFYLFVIQYKG"
     gene            781..>3061
                     /locus_tag="ACZO9R_06295"
     CDS             781..>3061
                     /locus_tag="ACZO9R_06295"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HIR0912710.1"
                     /translation="MALSRGMNQEASQDTDLGLLDATLMQNLQDRFCDANNVYLVCLS
                     RTRGVVTKAYGSKEELAYIHGLTGVERHMTLMNRLLDDGIESVLEEDCGNDLVKMCGV
                     SIKIGGKTAAIWIVIGIMDREGGDVPSYVMRTTPERYDKSLEFLETLSKQMFAVKMEE
                     LMAQEAVLKSRESETQMEAELHRNEVMTSIVKMLESENSFEEIVHDILRECCAYMEIS
                     RGALLKENKEHSKIEMICEYAAPGMDSVRGEWSGVSREKMPFFNGKPYMISSNSMMPE
                     VFRQFFDAHGLLAGIFLPVEVNTRAEMYLCFLEEHEERNWEVTDVKFVNDVRRIIQSI
                     LLKRIAKNSLASSYASLEAILENVGCGIYVKDPVSGRILYSNQRLRAFLPDDAAPDML
                     ENCWREESGGIDTDCFHEIYLEQEKRWFDYHRTGIHWVDGRSVFLYTIYEVTDKKLYQ
                     QKIERQANNDFLTGLYNRMRFEQDLAHCIEQTKEFGGEGALLYIDLDDFKHINDGLGH
                     QYGDILLKNISDSLRRIPGVENNCYRMGGDEFIIILAHRQIMMLQKILNDIRTLFSKP
                     WMLKGADYYCTMSMGVVRFPTEGDTVEELIKKADIALYAAKCSGKNRVEFYDENVEST
                     SFRRLDMEKNMRNATNNAFSEFEVVYQPIVDVTRESNPCVGAEALIRWNSGELGLIAP
                     TDFIPLAEYLGLINPIGAYVLEEACKRCKYWNDMGHPDYKVNVNLSVVQLLQNDIVEQ
                     VRSVIKRTGIVPENLTLEVT"
BASE COUNT          788 a          623 c          929 g          721 t
ORIGIN      
        1 cacaggcgca ccgggagacg acgccgtttt atttcagact ggaaataaag agccacatgc
       61 cgttagacaa aaagagcacg tttgccagac ggttttccgc ggagctggag cggctgtccg
      121 gcaggatgct tgtgaatgca ccatcggatt atgagattga actgcggctt ctggagaaaa
      181 gagacggcgg ttacgcctgc tttctgaaac ttcttacgat tgcgcatgga cgctttgcct
      241 accgcaaaaa tgcggtggca gcttcgattc atccggcgac ggcggcaatg cttgtggcgc
      301 tggcagagcc gtatttaaaa gaaaatgcgc agattctcga tccgttctgc ggtgtcggca
      361 cgatgttgat cgagcgggat atccgtgtcc ctgcgcggga aaaatacgga attgatatct
      421 ttggcgaggc aatccgggga gcgagggaga atgcatcata tgcgggggag aaaatcaatt
      481 ttatcaaccg tgattatttt gattttacac atgcatatct atttgatgag atcatcacca
      541 atatgcctgt ccggggaaga aagtcaaagg aagagacgga cgctttttac agcagatttt
      601 ttgaaaaatc agccggactt ttaaaagcgg acggaattct tgtgctctat tccaatgagc
      661 agggatttat cagaaaacag cttcgcataa gaagcgatta ccggctgctt gccgagcagt
      721 gtatccggga aaaggatcag ttttatctgt ttgtaattca gtataaagga tgaggaagat
      781 atggcacttt caagggggat gaatcaggaa gcgtcgcagg ataccgatct gggattgtta
      841 gatgcgacat tgatgcagaa tctgcaggac cggttttgcg atgccaataa tgtgtatctg
      901 gtgtgtctga gcagaacgcg cggagttgtc acaaaggcat atggttctaa ggaggaactg
      961 gcgtacattc acggactgac cggagtggaa cggcatatga cgctcatgaa ccggctttta
     1021 gatgacggaa tcgaaagtgt gctcgaagag gattgtggaa atgatctggt gaaaatgtgc
     1081 ggcgtgtcca tcaagatcgg cgggaagacg gcggcaatct ggatcgtcat cggaatcatg
     1141 gatcgggaag gcggggatgt tccatcttat gtcatgcgca cgacgccgga acggtatgat
     1201 aagtcactgg aatttctgga gacattgtcc aagcagatgt tcgccgtgaa gatggaagaa
     1261 ctgatggcac aggaggcggt gttaaagagc cgcgagtctg agacgcagat ggaagcggaa
     1321 cttcacagga atgaggtgat gacttccatc gtaaagatgt tagagtcgga aaacagcttt
     1381 gaggagatcg tgcacgacat tctgcgggaa tgctgcgcgt acatggagat ttcgcgcggt
     1441 gcgctcttaa aagagaacaa agagcactcg aagatcgaga tgatctgtga atatgcggcg
     1501 cctggaatgg actcagtgcg tggggaatgg tccggcgtgt cgcgtgagaa gatgcccttt
     1561 tttaacggaa aaccgtatat gatttcttca aacagtatga tgccggaggt gttccggcag
     1621 ttttttgatg cgcacgggct tttggcaggc atttttctgc cggtcgaggt caataccagg
     1681 gcggagatgt acctgtgctt tttggaagaa cacgaggagc ggaactggga agtgacggac
     1741 gtcaagttcg tcaacgatgt cagacggatt attcagagta ttcttctgaa gcgtattgca
     1801 aaaaattcgt tggcgagttc gtatgcgtcc cttgaggcga ttttagagaa cgtgggctgc
     1861 ggaatctatg tgaaggatcc ggtcagtgga aggattctct acagcaacca gcggctgcgc
     1921 gcatttctgc cggacgatgc cgcgccggac atgttggaga actgctggcg ggaagaatcg
     1981 ggcggcattg atacggactg cttccacgag atctatctgg agcaggaaaa acgctggttt
     2041 gactatcacc ggacaggaat ccactgggtc gacggaagaa gtgtattttt gtataccatc
     2101 tatgaggtga cggacaaaaa gctgtatcag cagaagatcg aacggcaggc aaacaacgat
     2161 tttttgaccg ggctttacaa ccgtatgcgg tttgagcagg atcttgcgca ctgcattgaa
     2221 cagacaaagg agttcggcgg cgaaggagcg ctgctctaca tcgatctcga tgattttaag
     2281 catatcaacg acggactggg acaccagtac ggggatatcc tgttaaaaaa tatatcggac
     2341 agcctgcgtc ggattccggg tgtggaaaat aactgttacc gcatgggcgg agatgagttt
     2401 atcattattc ttgcacatcg gcagatcatg atgctgcaga agattctgaa cgacatccgc
     2461 acgctgtttt ccaagccgtg gatgcttaag ggggcggatt actactgtac gatgagtatg
     2521 ggcgttgtgc gttttccgac agagggcgac acggtggagg agctgatcaa gaaggcggat
     2581 attgcgcttt acgccgcaaa atgttccggc aagaaccgtg tggagttcta cgatgagaac
     2641 gtggagtcca catcgttccg ccgcctcgat atggaaaaaa atatgcgcaa tgcgaccaac
     2701 aatgcatttt ccgagtttga ggtggtctat cagccgatcg tggatgtgac cagggaatcc
     2761 aatccgtgcg tgggtgcgga ggcgttgatc cgctggaatt ccggggaact gggattgatt
     2821 gctccgacgg attttatacc gctggcggag tatctggggc tgattaaccc gatcggtgcg
     2881 tatgtcctcg aggaggcgtg caagcggtgc aaatactgga acgatatggg gcatccggat
     2941 tacaaggtaa atgtcaatct ctccgtggtg cagctgctgc agaatgacat tgtagagcag
     3001 gtgcgctcgg tcatcaaacg gacggggatt gtaccggaga atctgaccct ggaggtcacg
     3061 g
//
LOCUS       DBMDQJ010000264         3060 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11528_length_3060_cov_1.585333, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000264 DBMDQJ010000000
VERSION     DBMDQJ010000264.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11528_length_3060_cov_1.585333"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1009)
                     /locus_tag="ACZO9R_06300"
     CDS             complement(<1..1009)
                     /locus_tag="ACZO9R_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3880 domain-containing protein"
                     /protein_id="HIR0912711.1"
                     /translation="MNLLFYRYGSICEPDIIEGFRELGNQVTEITLEIYNKNLGPLEG
                     LTALKNELFAHSYDFVFSINYYPFISEICNIFQIRYLCWTVDCPVMELYSDTVSNPWN
                     RIFLFDRAQYEEIAPQNPGCIFHLPLASNPSRWHNVLQKASPAEHAKYAGDVTFVGSL
                     YTEKCPYDRLQTDSAYLRGYLEGIMDAQRSIYGCYFLEDLLPDSIVAEFREALPGFYI
                     PPEKSIRDDRVAMAQIYLCAKISAQERVRTMQLLGNHYPVTLYTGSDSTGLPVQNKGL
                     VKTLTEMPLVFSNSKINLNITAKSIRSALPLRIFDILACGGFCLTNYQPELEDLFTVG
                     ED"
     gene            complement(1026..2198)
                     /locus_tag="ACZO9R_06305"
     CDS             complement(1026..2198)
                     /locus_tag="ACZO9R_06305"
                     /inference="COORDINATES: protein motif:HMM:NF024914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CgeB family protein"
                     /protein_id="HIR0912712.1"
                     /translation="MNILFCKHNSICEPGIIRALELLGHKLILPSCSFSHPDTDDAYD
                     AVLCEEIRKNSFDLVFSVNFIPVIATVCYRLQVPYFCWIVDSPVIQLYSHTLQYDSNY
                     VFLFDYTLYTEFCRKNPGHIFYLPLGCDLPHYDSIIPTPQDHANYDCDVSFVGSLYTE
                     KSPYHAIRTQLPSYIQGYFDGILHAQQLVYGYNFLCDIIPPDLMDTLDQLIDVSPSPG
                     YDLDKRTLLGNLLLNPMCTEMERIHLLNSIGEHFSLDLYTQSDTSPLKNIRLRGIASS
                     LDGMPKVFKCSKINLNLTAKGIQSGASLRIFDVLGCGGFLLSNYQSELPQLFEVGREL
                     VLFDSEADLLDKISYYLHHEDERREIATNGYQRIRQEYSYQARLALMLDVRHSLSI"
     gene            2392..>3060
                     /locus_tag="ACZO9R_06310"
     CDS             2392..>3060
                     /locus_tag="ACZO9R_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HIR0912713.1"
                     /translation="MLNGKTILVTGGTGSFGNAFTQYVLDHYTPKKIIIYSRDEYKQF
                     IMANKFRKYKDTLRFFIGDVRDKERLYRAFDGVDYVVHAAALKQVPACEYNPMEAVKT
                     NIDGAMNIVDAALDCGVKRVVALSTDKAVNPINLYGGTKLVSDKLFIAANAYAGAKDV
                     CFSIVRYGNVAGSRGSVIPFFRNIVKEGGTSLPITDYRMTRFWISLDEGVQLVIKAMS
                     EAKGG"
BASE COUNT          892 a          620 c          799 g          749 t
ORIGIN      
        1 gatcttcccc aacggtaaaa agatcctcaa gttccggctg gtaattggtg aggcagaaac
       61 cgccgcaggc aagaatatca aaaatgcgaa gcggcaacgc cgaccggatg gattttgcgg
      121 taatgttcag attgatcttg ctgttggaga agaccagagg catctctgtc aatgttttta
      181 caaggccttt attttggacg ggcagaccgg tggaatcact tccggtatat agcgtgaccg
      241 gataatgatt tcccagaagc tgcatggtgc gaacccgttc ctgcgcagaa attttggcac
      301 agagataaat ctgagccatc gcaacgcggt cgtcgcggat ggatttttcc ggcggaatgt
      361 agaatccggg caatgcctcg cgaaattcgg caacgataga atccggcagc aggtcttcca
      421 gaaagtagca gccgtaaatg ctgcgctgcg catccatgat accctcgaga tatccgcgca
      481 ggtaggcgga atcggtctgc agacgatcat aagggcattt ctcggtgtac agagagccga
      541 caaacgtgac atcaccggca tattttgcgt gctccgcggg ggacgccttt tgaaggacgt
      601 tgtgccagcg ggatggattg ctggcaagcg gcagatggaa aatgcagccg ggattttggg
      661 gagcaatctc ctcatactgg gcacggtcaa atagaaaaat acggttccac ggattgctca
      721 cggtgtcaga atatagttcc ataaccgggc agtccaccgt ccagcagaga tagcggatct
      781 ggaagatatt gcagatttcc gagataaagg gatagtaatt gatgctgaac acaaaatcgt
      841 agctgtgtgc aaacaattca ttcttcagag cggtaagacc ttccaggggt ccgagattct
      901 tattgtaaat ttccaatgtg atttctgtta cctgattccc gagttcccga aacccctcga
      961 taatatcagg ttcgcaaata cttccatagc ggtaaaataa cagattcata ggtacctcac
     1021 ttatattaaa tggataagga atgacgtaca tccagcatta gcgcaagccg cgcctggtaa
     1081 ctgtattcct ggcggattcg ctgataaccg tttgtggcaa tttccctgcg ttcatcttcg
     1141 tgatgcagat aataagaaat cttatcaaga agatcagctt cactgtcaaa gaggacaagc
     1201 tctctgccga cttcaaataa ttgcggtaat tccgactgat agttgctgag cagaaaaccg
     1261 ccgcaaccga gaacatcaaa aatgcgcaga gaagcgcccg actggatccc ttttgcggta
     1321 agattcaaat tgattttgct gcatttgaaa acttttggca ttccgtcgag agaggaggca
     1381 attccccgca gacggatatt tttgagtggg gacgtatcac tctgcgtata gagatccaga
     1441 gaaaagtgtt ctccgatgga attcagcaga tgaatacgct ccatctccgt acacataggg
     1501 tttaaaagca gatttccgag cagtgtgcgt ttatccagat cataaccggg agaaggggat
     1561 acatcgatca gctgatccag cgtatccatg agatccggtg gtatgatgtc acacagaaag
     1621 ttgtatccgt agacgagctg ctgcgcatgc aggatgccgt cgaaatatcc ctgtatataa
     1681 gaaggaagct gcgtgcggat agcgtgataa ggcgactttt ctgtataaag agaaccgaca
     1741 aaggaaacat cacagtcata atttgcgtga tcctgcggag tcggtataat agaatcataa
     1801 tgcggaaggt cgcagccgag cggcagataa aaaatatgcc ctggattttt tctacaaaac
     1861 tccgtataga gtgtatagtc aaaaagaaag acataattgc tgtcatactg taatgtatgc
     1921 gagtagagct gaatgaccgg gctgtccacg atccagcaaa aataaggaac ctgcagacga
     1981 tagcagacag ttgcaatcac aggaataaaa ttaacggaaa atacaagatc aaaagaattt
     2041 ttgcgaattt cctcacacag cacggcgtcg tatgcgtcgt ctgtatccgg atgggaaaag
     2101 gaacaggacg ggaggataag cttgtgcccg agaagttcca gagcacggat aatgccgggt
     2161 tcacagatgc tgttgtgttt acagaataat atgttcatga atatttcctt tcggacaaag
     2221 cgcaaaatct tttcataatt gcggttttat gacatttatt atatactaaa aaacggaaga
     2281 gtgacaagta tatgggacaa ttttagacag cgaaggttat gcagaagctg aatggttcga
     2341 tatatattat aaggcggcag gaggctgcag gagaaacagg aggttgtaga aatgttaaac
     2401 ggtaagacaa tattagtgac aggtggtacg ggatcttttg gcaatgcatt tacacagtat
     2461 gtgctggatc actatacacc gaagaagatt atcatttatt cccgcgatga gtataagcag
     2521 ttcattatgg cgaacaagtt cagaaaatat aaagacacac tgcggttttt tatcggagat
     2581 gtccgtgata aagaaagact gtaccgggcg tttgatggag tggattatgt ggtacacgca
     2641 gcagcattaa agcaggttcc ggcgtgtgaa tataatccga tggaggctgt gaaaacgaat
     2701 atcgacggag ccatgaacat tgtggatgca gcgctcgact gtggtgtaaa gcgagtggtt
     2761 gcactctcga cagacaaggc ggtcaatccg atcaacctgt acggcggtac caagcttgtg
     2821 tcggataaac tgtttattgc ggcaaatgca tatgcgggag caaaggatgt ttgcttctct
     2881 attgtaagat acggaaatgt tgccggaagc aggggatctg tcattccgtt tttccgcaat
     2941 attgtgaaag agggtggcac ttctctgccg attaccgatt atcgcatgac acgcttctgg
     3001 atcagcttgg acgagggcgt acagctggtt attaaggcaa tgtccgaagc gaagggagga
//
LOCUS       DBMDQJ010000265         3057 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11542_length_3057_cov_1.240189, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000265 DBMDQJ010000000
VERSION     DBMDQJ010000265.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11542_length_3057_cov_1.240189"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="ACZO9R_06315"
     CDS             <1..1175
                     /locus_tag="ACZO9R_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR0912714.1"
                     /translation="CPEHIALSLEASKRSMVLLKNDNHFLPLKQEQVHTIAVIGPNAN
                     SRAALVGNYEGTSSRYITPLEGIQEYTGEKTRVLYAQGCHLYKDQVEFLGEPKDRFKE
                     ALIAAERADVIVMCLGLDAGIEGEEGDAGNEYASGDKLGLKLPGLQQELLEAVAAVGK
                     PIVLTVLAGSALDLSWAQEHAQIRAILDCWYPGARGGKAIAEALFGEFSPCGKLPVTF
                     YEGTEFLPDFTDYSMAGRTYRYTDRHVLYPFGYGLTYSQIRYSDAHADVTDFGILEPV
                     TVHVTVENTGTYPVQEAVQVYVRFSEREAYDPGYQLKGIRSVALECGEKKEVCITLST
                     RDFALISEEGKCLVHPGSYEIAVGGQQPDERSSRLTGQTVSTLFICKTGNVTEVEY"
     gene            1239..2993
                     /locus_tag="ACZO9R_06320"
     CDS             1239..2993
                     /locus_tag="ACZO9R_06320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912715.1"
                     /translation="MKRILHAVEAWMDDFKIKNKFYILYILCVLVPLIVTDSAVFSVV
                     MKSEREAREHEMANIANAVAYSISNTVNNAAEIGKSIYTSKYINRFLANTYSDPLDYV
                     VSYQDFFKDTLFESGLGMNNMVITMYADNDTIVNGGKVCNMKEIRGTEVYRYFQDNGL
                     NKGLYFQYDDSRAPSVEPQRRMLFFQKLDFYAENNMEKVLLINIDYSAVNRTLEKMNY
                     DTDVFICQGDKIVLSNGRYSSIGKEFQTFDQTGRVGYCQELEFYGMDLDIYVMQPKRQ
                     LWTEIRKNLPLIGFLIVVNAILPLLLVRGFNRSFTQRISELSKVFETVGGEHLVPISY
                     VRGKDEIGSLMSNYNRMVARTNELIQIVYKNKIKEQEMLVERQNAELLALHSQINPHF
                     LFNALESIRMHSLLKNEYETADMVEKLALMQRQYVEWGNDSVEIRREMEFVRAYLGLQ
                     KYRFGERLSYSLEVEEDCLDIKIPKMTVVTFVENACVHGIEAKTAPGWIFVRICQKQK
                     ALYLEVEDTGCGMDASQMQELKERMLNASIDMLKDGGRVGIINACLRLKMVFGEQVAF
                     DIEGEQGVGTTVSIRMPL"
BASE COUNT          875 a          559 c          894 g          729 t
ORIGIN      
        1 agtgccccga acatattgca ctgtccttag aagcctcgaa aagaagtatg gtgctgctta
       61 agaatgacaa ccattttctt ccgctgaagc aggaacaggt ccataccatt gcagtgatcg
      121 gaccaaacgc gaactccaga gcagcgctgg ttggaaatta tgaggggaca tcctccagat
      181 acattacacc gctggaggga attcaggaat acaccggaga gaaaacccgc gtcctatatg
      241 cgcagggctg ccatctgtat aaggatcagg tagaattcct cggggagccg aaggaccggt
      301 ttaaggaagc gctgattgct gcggagcggg cagatgtcat tgtgatgtgt ctgggattgg
      361 atgcgggcat cgaaggcgaa gagggagatg cgggaaatga gtatgccagc ggtgacaagc
      421 tgggattaaa gcttcccgga ctgcagcagg aacttttaga ggcggtcgca gcagtcggaa
      481 aaccgattgt gctcaccgtg ctggcgggaa gtgcactgga tctgtcgtgg gcgcaggaac
      541 atgcacagat acgggcaata ctggattgct ggtacccggg agcgagaggt ggaaaagcaa
      601 tcgcagaggc attgttcgga gaattctctc catgcggaaa actgccggtt accttttatg
      661 aaggaacgga atttcttccg gattttacgg attattccat ggcaggacgt acctaccggt
      721 atacggacag acatgtgctc tatccgtttg gatatggtct tacctattcg cagatccgtt
      781 attctgatgc acatgcagat gtcacagatt ttggaatact ggagccggta acggtgcatg
      841 taacggtgga gaataccggt acctacccgg tacaggaagc ggtacaggta tatgtcagat
      901 tcagtgaaag agaagcttac gatcccggct atcagctgaa gggaatccgg tcggttgcac
      961 tggaatgtgg ggaaaagaaa gaggtttgta tcaccctttc tacgcgtgat tttgcactga
     1021 tctccgagga gggaaaatgt ctggtacacc cgggaagcta tgagatcgca gtcggagggc
     1081 agcagccgga tgagagaagc agccgtctga ccggacagac ggtgagcaca ctttttatat
     1141 gcaaaacggg aaatgtgacg gaagtggaat actgatgctg ccggtatccg ttgcactcga
     1201 tgaaaaacaa tgttagtata aaaatactgg gagaggatat gaagagaatt ctgcatgcgg
     1261 ttgaagcctg gatggatgac tttaaaataa aaaataaatt ttatatcctg tatatcttgt
     1321 gcgtgctggt tccgctgatt gtgacggaca gtgcggtatt ttctgttgtc atgaagtctg
     1381 agcgggaagc cagagagcat gagatggcga atattgccaa tgcagttgca tacagtattt
     1441 caaatacggt caacaatgca gcagagatcg ggaagagtat ttatacgagc aaatatatta
     1501 accgtttttt ggcgaatacc tacagcgatc cgttggacta tgtggtgagt tatcaggatt
     1561 tttttaaaga taccctgttt gagagcggac ttggcatgaa caacatggta atcacgatgt
     1621 atgcggataa tgacacgatc gtgaatgggg gaaaggtatg taatatgaag gaaatacgcg
     1681 gtacagaagt gtaccgctat tttcaggata acggattgaa taagggatta tatttccagt
     1741 atgatgacag cagggcacct tcggtggagc cgcagagacg gatgcttttt ttccagaagc
     1801 tggactttta tgcggaaaat aatatggaaa aggtgctgct gatcaacatc gattacagtg
     1861 cagtaaaccg gacattagag aaaatgaatt atgacacgga tgtatttatc tgccagggag
     1921 ataagattgt cttatcaaat ggcagatatt ccagtatcgg aaaggaattt cagacatttg
     1981 accagacggg cagggtcgga tactgtcagg aactcgagtt ttacggaatg gatctggata
     2041 tttatgtcat gcagcccaaa cgccagctgt ggacggaaat ccggaaaaac ctgccgctga
     2101 ttggattctt aattgttgta aatgcgattc tgccactcct tctggtgcgg ggatttaacc
     2161 ggtcatttac gcagagaatc agcgaactca gcaaagtgtt tgagacagtc ggcggggaac
     2221 atctggtgcc gatttcgtat gtgcgtggaa aagatgagat tggaagcctg atgagcaatt
     2281 acaaccgcat ggttgcgaga acaaatgaac tcattcagat tgtgtataag aataagatta
     2341 aagaacagga gatgctggtg gaaagacaga atgcagaact gctcgcgctg cacagccaga
     2401 tcaatccgca ttttctgttt aatgcgttgg agagcatccg catgcacagc ctgttgaaaa
     2461 acgaatatga gacggcagac atggtggaaa agctggcgct gatgcagcgt cagtatgtgg
     2521 aatggggaaa cgacagtgtc gagatccgca gggaaatgga atttgtgaga gcgtatctgg
     2581 ggcttcagaa gtatcggttt ggagagcggt taagttacag tcttgaagtg gaagaggatt
     2641 gtctggacat taaaattccc aaaatgacag ttgtcacctt tgtggaaaat gcctgtgtac
     2701 atggaattga ggcaaagaca gctcccggct ggatttttgt gcggatctgc caaaagcaga
     2761 aggcacttta tctggaagtg gaagataccg gatgcggcat ggacgcctcc cagatgcagg
     2821 aattaaaaga gcgcatgcta aacgccagta ttgatatgct aaaggatggc ggaagagtgg
     2881 gaatcatcaa tgcatgtctg cggctgaaaa tggtattcgg ggagcaggtg gcatttgata
     2941 tagaggggga gcagggtgtc ggtacgacag tgagtatccg gatgccattg tagagaaggg
     3001 aaggaaaaag atgggggaag cattgttaaa ggtattgctc gtggatgatg agccgtt
//
LOCUS       DBMDQJ010000266         3042 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11623_length_3042_cov_1.363142, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000266 DBMDQJ010000000
VERSION     DBMDQJ010000266.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11623_length_3042_cov_1.363142"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="ACZO9R_06325"
     CDS             complement(<1..687)
                     /locus_tag="ACZO9R_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP cyclohydrolase"
                     /protein_id="HIR0912716.1"
                     /translation="MKMMSLAKELSSNTYPGRGIVIGRSGDGKYAVTAYFIMGRSENS
                     RNRVFVEDGEGIRTQAFDPSKLTDPSLIIYAPVRVLGNKTIVTNGDQTDTIYELMDKQ
                     QTFEQALRTREFEPDAPNYTPRISGIMHIENNTYNYAMSILKSNNGNPDACNRYTFAY
                     QNPVAGEGHFIHTYMGDGNPLPSFEGEPKLVGIEGDIDTFTNTVWENLNADNKVSLFV
                     RYIEIETGKYE"
     gene            complement(810..2048)
                     /locus_tag="ACZO9R_06330"
     CDS             complement(810..2048)
                     /locus_tag="ACZO9R_06330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0912717.1"
                     /translation="MAEIRKIIFIKNAVETLGYFSEQIARELERNGYETCFIDYERMY
                     ESMDVMLHFLDREETALVTFNFIGLRGEAVFQTESGRSIWQEENLPVLNILVDHPLYY
                     HSCLKEAGERMRVFCVDREHVGYVRRFYPGVKVEFLPLAGNELLFSHGDGEHGTHRAD
                     VPEPVLYGQRTFDLVFAANYVSMEMLEEKVKALDDDYRIFYRRITEDLIADPAQSVDA
                     VMERHIRNELGAVPEEQLCAAMSGMIWIDLFVRSYFREKVVQTLADAGIIVRVFGADW
                     EKIHCKKPQNVRTSGGKVNSAACVQAMRDARIALNVMPWFKDGAHDRVFTAMLQGTAA
                     LTDDSRYLREECRDGENICFYSLRALQQLPDQVVSLLEDPVRMAELAERGYRMAEKRH
                     RWKNRAGLLMQTLCNIDKYN"
     gene            complement(2171..>3042)
                     /locus_tag="ACZO9R_06335"
     CDS             complement(2171..>3042)
                     /locus_tag="ACZO9R_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HIR0912718.1"
                     /translation="LMGEILLELAEQSKDRDSADCMKLVVFCNAPDDNPFMAGAFHGV
                     TEADAIINVGVSGPGVVKTALEKVRGENFEVLCETIKKTAFKVTRVGQLVAQEASRLL
                     NIPFGIVDLSLAPTPAIGDSVADILCEIGLEYAGAPGTTAALAMLNDQVKKGGVMASS
                     YVGGLSGAFIPVSEDQGMIDAVQAGAITLEKLEAMTCVCSVGLDMIAIPGDTKATTIS
                     GMIADEMALGMVNQKTTAARLIPVIGKGVGDTVEFGGLFGYAPIMPVNQFSCDAFINR
                     GGRIPAPIHSFKN"
BASE COUNT          655 a          941 c          660 g          786 t
ORIGIN      
        1 ctcgtatttt ccggtctcaa tctcaatgta acgcacaaac aaagatactt tattgtctgc
       61 gtttaagttc tcccagaccg tgtttgtgaa ggtgtcgatg tctccctcga tgccgacaag
      121 cttcggctct ccctcaaaac tcggcagcgg attgccgtct cccatgtatg tatggataaa
      181 atgtccctcg ccggccaccg gattctggta tgcaaacgta tagcggttgc aggcgtccgg
      241 attgccgttg ttactcttta aaatagacat cgcgtagttg taggtattgt tctcgatgtg
      301 catgataccg gaaatccgcg gtgtatagtt cggtgcgtcc ggctcaaact ctctggtgcg
      361 cagcgcctgc tcgaacgtct gctgcttgtc catcagttcg taaatcgtat cggtctgatc
      421 gccgtttgtc acgatcgtct tgttgccaag cacgcggacc ggtgcataaa tgatgagact
      481 cggatctgtc agcttggacg gatcaaatgc ctgtgtgcgg attccttcac catcctccac
      541 aaagaccctg ttgcggctgt tctcgcttct tcccatgata aagtatgcgg ttaccgcata
      601 ttttccatcc ccggatctgc caatcacaat tcctcttccc ggatatgtat tggacgaaag
      661 ttccttcgca agtgacatca tcttcatgat tttcctcctt cacatcacac attgctacat
      721 tgattgcctt ctttcatctt atcatgcgga ttttcttttt acaaggaatg cgttgtcatt
      781 agtattatga atttatttac tgaattctat tagttatact tatctatatt gcaaagcgtc
      841 tgcatcagaa gcccggcgcg gttcttccac ctgtgccgct tttctgccat ccgatatccc
      901 cgctccgcca gctctgccat ccgcaccgga tcctcaagca ggcttaccac ctgatccgga
      961 agctgttgta acgccctcag ggagtaaaaa cagatatttt ccccgtcccg gcactcttcc
     1021 cgcagatacc ggctgtcgtc cgtgagcgcc gccgttccct gcagcatcgc cgtaaagacg
     1081 cggtcatgtg ctccgtcttt aaaccagggc atcacgttta acgcgatcct ggcgtcacgc
     1141 atcgcctgca cacacgccgc cgagttcact tttccgccac tcgtccgcac gttctgcggc
     1201 tttttgcagt gaattttctc ccagtctgct ccgaataccc gcactatgat accggcatcc
     1261 gcgagcgtct gcactacttt ttcccggaaa tagctgcgga caaacagatc aatccagatc
     1321 atcccggaca tcgctgcgca aagctgctct tccggcactg ccccaagctc gttgcggatg
     1381 tgccgctcca taaccgcatc tacagactgc gcgggatccg cgatcagatc ttccgtgatc
     1441 cgccggtaaa agatccgata atcgtcatcc agcgccttta ctttttcctc cagcatctcc
     1501 atcgacacat agttggccgc aaatacaagg tcaaaggttc tctgcccata cagcacaggc
     1561 tctggcacat ccgctctgtg cgttccgtgt tccccgtctc catgcgaaaa aagaagttca
     1621 ttgcccgcca gcggcaggaa ctccaccttc acccccgggt aaaaacgcct cacatagccg
     1681 acgtgctctc tgtccacaca gaatacccgc atccgctccc cggcttcctt cagacagctg
     1741 tgatagtaga gcggatgatc caccaagata tttaagacgg gaaggttctc ctcctgccag
     1801 atactgcgtc cgctctccgt ctgaaacaca gcctccccgc gcagcccgat gaaattaaag
     1861 gtgacaagtg ccgtctcttc ccggtccaga aaatgcagca ttacatccat cgactcatac
     1921 atccgctcat agtcgataaa gcaggtctca tacccgttcc tctccagttc tcgtgcaatc
     1981 tgttcggaaa aatatcccag cgtctccacc gcatttttta tgaaaatgat ttttctgatc
     2041 tctgccattc tgtgcttcct ctgtccggat aaaggaaaaa caacctccct gaatcatcct
     2101 ttcggattct gtctgatcag agaaggttgt tttctatcac ttattttcca tgactcatgt
     2161 tcgttatatt ttaattcttg aagctgtgga tcggcgccgg gattctgccg ccgcggttga
     2221 tgaacgcgtc acaggagaac tggttcaccg gcatgatcgg cgcatagcca aacagtccgc
     2281 caaattctac cgtatcgccc accccttttc cgataaccgg aatcaggcgc gccgcagtcg
     2341 tcttctggtt gaccatgcca agcgccatct catcggcaat cataccggag atggtcgtcg
     2401 ccttggtgtc tcccggaatc gcaatcatat cgagtcccac ggaacatacg caggtcatcg
     2461 cctcaagctt ctcaagtgtg atcgctcctg cctgtaccgc atcgatcatg ccctgatcct
     2521 cgctgaccgg aataaaagcg ccacttaatc cgccgacata ggaggaagcc atcacgccgc
     2581 cctttttcac ctgatcgttc agcatggcga gcgccgcagt cgttcccggt gctcccgcat
     2641 actcaagtcc gatctcgcag agaatgtccg ccacggaatc gccgatcgcc ggtgtcggcg
     2701 ccagagacag atccacgata ccaaacggga tattaagaag tctggatgcc tcctgtgcca
     2761 ccagctgtcc gactctcgtc accttaaacg ccgtcttctt aatggtctca cagagcacct
     2821 cgaagttctc gccgcgcact ttttccagag cagtcttcac cactcccggt ccgctgacac
     2881 cgacgttgat gatcgcatcc gcctccgtca caccgtggaa cgcgcctgcc ataaacgggt
     2941 tgtcatccgg tgcgttgcaa aatacaacaa gcttcatgca gtccgcagaa tcgcggtcct
     3001 ttgactgctc cgcaagctct aagagaatct ctcccatcag ct
//
LOCUS       DBMDQJ010000267         3036 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11649_length_3036_cov_1.647019, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000267 DBMDQJ010000000
VERSION     DBMDQJ010000267.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3036)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3036)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3036
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11649_length_3036_cov_1.647019"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="ACZO9R_06340"
     CDS             complement(<1..1396)
                     /locus_tag="ACZO9R_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912719.1"
                     /translation="MDKRTLSDMARCNRTAMICHLVEASMITITFIAQLLVGSRGLLY
                     SLLVVVLAMGPPLMELFFWSRDKESPAIKHLVAQGFAVMYTVILFTTTNNMLFLYVFP
                     MIVVISVYNDKAYALKVNIGVVIENLLVVILGATTGRFGFRDMSSGVIQILAVLLFCA
                     YSYIAAATSETNSREKLQQVKDAQGETEKVLGDVSRNADVMRRGINEIHAKVEQLQSA
                     SETTKDAMGQVTIGATDTAEAVQRQLAQTETIGEKVGLVSTVASDITERMEKTLSVLE
                     KGKADMDTLVGEVEVSVRNSANAADKLETLNQYISEMNTIVELINGVTSQTSLLALNA
                     SIEAARAGEAGKGFAVVASEISELATQTKSATTNITQLIGNVSGSITEVVTVIREMID
                     GINEEKEMSGNASASFGTIEEHTYAIRDNVARLTESVSQLEAANQEIADSVQTISAVS
                     EEVSAHANETLAAEQ"
     gene            complement(1537..2280)
                     /locus_tag="ACZO9R_06345"
     CDS             complement(1537..2280)
                     /locus_tag="ACZO9R_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR0912720.1"
                     /translation="MELWTEQIRQAHAGDKDARNRLVAENLGLVHTVTRRFEHRGHDR
                     EELFQIGCIGLMKAIDKFDVSLQLAFSTYAVPMITGEIRRFLRDDGMVKVSRTLKENG
                     YKIRRAGEMLTRELGREPTMEELAEAAGLEHEEIVLALEANREVASIYQPVYERDGEE
                     LLLIDQMEGSGGQSAEREEPEKEAVLNHILVEQLLETVGERERRLLQLRYYEGKTQCE
                     VAELLSMSQVQVSRLEKKLLLQLRERVRM"
     gene            complement(2286..2720)
                     /gene="spoIIAB"
                     /locus_tag="ACZO9R_06350"
     CDS             complement(2286..2720)
                     /gene="spoIIAB"
                     /locus_tag="ACZO9R_06350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042174 - negative regulation of
                     sporulation resulting in formation of a cellular spore
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HIR0912721.1"
                     /translation="MHSKNEVELSFDARSVNEGFARMAVAAFITELNPTMDELSDVKT
                     AVSEAVTNAIIHGYDNPEEKVHMTCRIMGREVEIIVCDTGRGIEDVARAMEPFYTTKP
                     ELERSGMGFAFMEAFMDEVQVQSAPGKGTSVRMKKYIGVQEG"
     gene            complement(2713..>3036)
                     /locus_tag="ACZO9R_06355"
     CDS             complement(2713..>3036)
                     /locus_tag="ACZO9R_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR0912722.1"
                     /translation="NCLRVYVPRELDHHEADRLKQEADFFIETGQVKTLVFDFAGTEF
                     MDSSGIGVIIGRSRKMGYFGGEVYAEHLSGRILKIFKVSGLHTLIRTSVPKQQKEREG
                     ENHNA"
BASE COUNT          629 a          996 c          612 g          799 t
ORIGIN      
        1 cttgctccgc cgcaagcgtc tcgttcgcat gtgccgatac ctcctccgac actgccgaga
       61 tcgtctgcac ggaatcagca atctcctgat ttgccgcctc aagctgtgaa acgctctccg
      121 tcagtctcgc cacattatcg cggatcgcat aggtatgttc ctcaatcgta ccaaagcttg
      181 ccgacgcatt tccggacatt tccttttcct cattgattcc gtcaatcatc tcacggatga
      241 ctgtcacaac ctccgtgata gaaccggaca cattgccgat cagctgtgtg atgttggtcg
      301 ttgcgctctt ggtctgggtt gcaagctccg agatctcgga tgccacgacc gcaaaccctt
      361 ttcctgcctc tcctgcgcgt gccgcctcaa tacttgcatt taacgccagc aggctggtct
      421 gggaagtcac accgttgata agttctacaa ttgtattcat ctcactgata tactgattca
      481 gcgtctcgag cttatccgcg gcattggcac tgtttcgcac agatacttca acctctccca
      541 ccagggtatc catatccgct tttccctttt ccagcacaga aagcgtcttt tccatgcgct
      601 cggtaatatc cgatgcaacc gtactgacaa gccctacttt ctctccgatt gtctccgtct
      661 gtgccagctg tctctgcact gcctccgccg tgtccgtcgc gccgatggtt acctgtccca
      721 tcgcgtcctt ggtcgtctcc gatgccgact gcagctgctc cacttttgca tggatctcat
      781 tgattccgcg tctcatcaca tccgcattcc gggagacatc cccgagaacc ttttcggtct
      841 cgccctgcgc atcctttacc tgctgcagct tctccctgct gttcgtctcg gaagtcgccg
      901 ctgcgatata ggaatacgcg cagaacaaaa gtaccgccag aatctggatc acgccggaac
      961 tcatatcacg gaaaccgaat ctgccggttg tcgctccaag aatcacaacc agaagattct
     1021 ctatcacaac cccgatattg accttgaggg catacgcctt gtcattgtat acggaaatca
     1081 caacaatcat cggaaataca tacaaaaaca gcatgttgtt ggtcgtcgta aacaaaatga
     1141 ccgtgtacat cacagcgaat ccctgcgcca ccaggtgctt gatcgccgga ctttccttgt
     1201 ccctgctcca gaaaaaaagc tccatcagag gcggtcccat cgccagcaca acaaccagca
     1261 gagagtacag cagtcccctg ctgcccacca gaagctgtgc gataaacgta attgtaatca
     1321 tcgacgcctc caccagatgg cagatcatag ccgtccggtt gcagcgcgcc atatcagata
     1381 acgttctttt gtccatttgt cattcctccg tacaccttga caccattctt ttactctaaa
     1441 gtatcatttt tttcgccctg tcgcaatatt tgtaataaaa actcgtccaa tttttaacat
     1501 tttttgaaaa ttggacaaat ccaactttct tttctgtcac atgcggacgc gctcacgcag
     1561 ctgcagcagc agcttttttt ccaggcggct tacctgcacc tgactcatcg acagcagttc
     1621 tgccacctcg cactgtgtct ttccctcgta gtatctgagt tgcagcagac ggcgctcacg
     1681 ctcgccgacg gtctccaaca gctgttccac cagaatgtgg tttaggaccg cctccttctc
     1741 cggctcctcc cgctccgcgc tctgccctcc gcttccctcc atctgatcga tcagcagcag
     1801 ctcctccccg tcgcgctcat agaccggctg ataaatggac gccacctccc ggtttgcctc
     1861 aagtgccagc acgatctcct cgtgctccag cccggcggct tccgccaatt cctccatcgt
     1921 cggctctctc cccagttccc tggtaagcat ttctcccgcc ctgcggattt tatagccgtt
     1981 ctcctttaag gttctgctga cttttaccat accgtcatcc cgcagaaaac gcctgatctc
     2041 cccagtaatc atcggtaccg cgtaggtgga aaaggcaagc tgcagcgata cgtcaaattt
     2101 gtcgatagcc ttcatcagcc cgatgcagcc gatctggaac agttcctccc tgtcatgtcc
     2161 tctgtgttca aagcgcctcg tcaccgtgtg caccagtccc aagttttctg ccaccagccg
     2221 gttccttgcg tccttgtccc ccgcgtgagc ctgtctgatc tgttccgtcc aaagctccat
     2281 aacgttcagc cttcctgcac gccaatgtat tttttcatgc gcacgctggt tccttttccg
     2341 ggcgcagact gaacctgaac ttcatccata aacgcttcca tgaacgcaaa tcccatcccg
     2401 gagcgctcca gttccggctt tgtggtgtag aacggttcca ttgcccgcgc cacatcctca
     2461 atccccctgc ccgtatcaca gacgatgatc tccacttccc gtcccatgat cctgcatgtc
     2521 atatgtacct tttcctccgg attgtcatac ccgtgaatga ttgcattggt caccgcctcc
     2581 gacacggccg tcttgacatc cgagagttca tccatcgtcg gattcagctc tgtgataaat
     2641 gcagccaccg ccattctggc aaatccctca ttgacggaac gcgcatcaaa acttaattcc
     2701 acttcattct tactatgcat tgtgattctc cccttcccgc tctttctgct gcttaggtac
     2761 gcttgtccgt atcagcgtgt gaagtccgga caccttaaaa atcttaagga ttctgccgct
     2821 taaatgttcc gcatacacct caccgccaaa atatcccatc ttccggctcc gcccgataat
     2881 caccccgatt ccggagctgt ccatgaactc cgttcccgca aaatcaaaca cgagcgtctt
     2941 cacctgaccg gtctcaataa agaaatccgc ctcctgtttc aggcggtctg cctcatgatg
     3001 atcgagttcc ctcgggacat acacccgcag gcagtt
//
LOCUS       DBMDQJ010000268         3034 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11659_length_3034_cov_1.574497, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000268 DBMDQJ010000000
VERSION     DBMDQJ010000268.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11659_length_3034_cov_1.574497"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(183..833)
                     /locus_tag="ACZO9R_06360"
     CDS             complement(183..833)
                     /locus_tag="ACZO9R_06360"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR0912723.1"
                     /translation="MWDRFFDLDNVVWRTIDKIGKIFLLNLLWLICSLPVFTIGASTT
                     ALIYTSMKLSDNEGYWHQNFFSSFKENFKQATALFFIYLAAGAFLAGDFLLGGRAGNS
                     FGQLMQGVAAILFVPYFLSLLYVFGVQAKFVNPVKATIRYSFFVAMRNMKTTIQLAIL
                     MILFVWVNTTTLLANFLTLIYGIGLMGYFYGMYYKHTFAKYIEAVEEGSADEEQER"
     gene            complement(823..1668)
                     /locus_tag="ACZO9R_06365"
     CDS             complement(823..1668)
                     /locus_tag="ACZO9R_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR0912724.1"
                     /translation="MQLGIRLHDIKKAPLEERLAIAHEQGFACGHLALAKVISEYPVD
                     DGALTPGYAMYLKKIFAENQLDVAVLGCYLNLANPNPASLAKNTHRYLAHIRFASLLG
                     AGVVGTETGAVNEEYRFEERNHSEEALKIFINNLCPVVSYAEKMGVIVAIEPVYKHIV
                     CNPKRARKVLDAIGSPNLQIIFDPVNLLDICNYKDRDAIIAEAIDVLGEDIAMVHIKD
                     FKVENNSLVSGAAGTGEMNYDRIIRFMKERKPYIHATLENTVPENAVASRKYIQRLWE
                     ECHVG"
     gene            complement(1725..>3034)
                     /locus_tag="ACZO9R_06370"
     CDS             complement(1725..>3034)
                     /locus_tag="ACZO9R_06370"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HIR0912725.1"
                     /translation="GVDIFGAKGDGVSDDTTFILAAIMACPPESRVLIPAGTYKITSL
                     FLKSGISVELAKGAELLADTDRSHYAILPGLIESYDETGDYNLGTWEGNPLPMFAGII
                     SGIDVSDVTLYGEGSINGAANHENWWKNEKVMVGAFRPRMLSLNRCRNIRVQGLYFHD
                     SPAWVIHPYFSDELLFCNLIVENPAKSPNTDGLDPESCRDVTICGVRFSLGDDCIAVK
                     SGKIYMGRRYKTPSSNIHVYQCLMEHGHGAVTVGSEMAGGVNNLIVEKCRFYHTDRGL
                     RIKTRRGRGKDAILDNIIFRDLMMEQVMTPFTANAFYFCDPDGRTEFVQSREPYPVDD
                     GTPSMKRFCFENITAKDCHVAASYFDGLPEQKIEQITMRNVSVSFAGQAKCDVPIMSN
                     GVEACCKKGLYARNVKKLVLDNVSIEGCEGEEIELHNVDEVER"
BASE COUNT          712 a          893 c          676 g          753 t
ORIGIN      
        1 cagctgcggc atggttagaa ataccggatt ttgcggtcag aatttgccgc cgcatgttta
       61 tctggaactt ttaagtatct atttcttgta atattactta tcaaaccaaa gcacaatatg
      121 cttattgcga caattccttt tcattatgct cgttttcgtc ggaacaactt cgtgtccgtc
      181 cgctaacgct cctgctcttc gtctgcggag ccttcttcca ccgcctcgat atacttggcg
      241 aacgtatgct tatagtacat tccatagaaa taccccatca gaccgattcc atagatcagc
      301 gtcaggaaat tggcaagcag cgtggttgtg ttcacccaga caaacagaat catcaagatt
      361 gcaagctgga tcgtcgtctt catgttgcgc attgcgacaa aaaaggaata ccggatcgtc
      421 gccttgaccg gattcacaaa ctttgcctgc acgccgaaca catagaggag cgaaagaaaa
      481 tacggtacaa acagaattgc cgccactccc tgcatcagct gcccgaagga attgccggca
      541 cgaccgccca acaggaagtc gcccgccagg aatgcacccg ctgccagata aataaaaaac
      601 agcgccgtcg cctgtttaaa gttctcctta aacgacgaga aaaaattctg atgccagtac
      661 ccttcattgt ccgaaagctt catgctggtg taaatcaggg cggtcgtcga tgccccgatc
      721 gtaaacaccg gcagggaaca gatcagccag agcagattca ataaaaatat ctttccgatt
      781 ttatcaatgg tacgccaaac cacgttgtcg agatcaaaaa atctatccca catggcactc
      841 ctcccacaat ctctgaatgt atttgcgcga tgccacggca ttttccggca ccgtgttctc
      901 taaggttgca tggatatacg gcttgcgctc tttcataaag cggatgatcc tgtcgtagtt
      961 catctcgccg gttccggctg cccccgatac caggctgtta ttctccacct taaaatcctt
     1021 gatatgtacc atcgcaatat cctcgccaag cacatcgatc gcttctgcta ttatagcatc
     1081 cctgtccttg taattgcaaa tatcaagcag attgaccgga tcgaaaatga tctgaaggtt
     1141 cggtgaaccg atcgcgtcaa gcacctttct cgcccgcttc ggattgcaga caatatgctt
     1201 gtacaccggc tcgatcgcaa cgatcacgcc cattttttcc gcataggaaa ccaccggaca
     1261 caggttgttg ataaagatct ttagcgcctc ctcggaatga ttgcgctcct caaaacggta
     1321 ttcctcattc actgctccgg tttccgtgcc gaccacaccc gcaccgagca gcgatgcaaa
     1381 ccggatatgc gccagatagc ggtgcgtatt ctttgcaagg cttgccggat tcggattggc
     1441 gagattcaag tagcatccaa gcactgccac atccagctga ttctctgcaa aaatcttctt
     1501 taaatacatg gcatagcccg gcgtcagggc accgtcatcc accggatact ccgaaatcac
     1561 ctttgccagc gccagatgtc cgcaggcaaa tccctgctca tgtgcaatgg caagccgctc
     1621 ctcaagcggc gcctttttga tatcatgtaa acgtattcca agctgcattt ttctatcctt
     1681 tccgggcttt cttcctcctg cccgcccaat gttccggccg tcttctaccg ctcaacctca
     1741 tccacgttgt gaagttcgat ctcctctccc tcgcatccct caatggagac gttgtccagc
     1801 acaagcttct tcacgttccg cgcatacaat ccctttttgc agcatgcttc cacaccgttt
     1861 gacatgatcg gcacgtcgca ttttgcctgc ccggcgaaag aaacggatac gttgcgcatg
     1921 gtaatctgtt cgatcttctg ctccggcaga ccgtcaaagt aggatgctgc cacatggcag
     1981 tctttcgccg tgatattttc aaagcagaag cgcttcatgg acggtgtgcc gtcatccacc
     2041 ggatacggtt ctctcgactg cacaaactcg gttcttccgt ccgggtcgca gaaatagaag
     2101 gcattcgcag taaacggcgt catgacctgc tccatcataa gatcccggaa gataatattg
     2161 tccagaatcg catccttgcc gcgtcccctg cgcgtcttga tacgcagacc gcggtcggta
     2221 tggtaaaacc ggcatttctc cacgatcaga ttattgacgc cgcccgccat ctcactgccg
     2281 acggtgaccg cgccgtgtcc atgctccatc aggcactgat agacgtgaat attggaggat
     2341 ggcgtcttgt atcttcttcc catgtagatc tttccggatt tcactgcgat acagtcatcc
     2401 ccgagggaga agcgcacacc gcagatcgtc acatcgcggc aggattccgg atcaagaccg
     2461 tccgtattcg gtgactttgc cggattctcc acgatcaggt tgcagaacag cagctcgtcg
     2521 ctgaagtacg gatgaatgac ccacgccggg ctgtcatgga agtaaagtcc ctgcacacgg
     2581 atgttcctgc agcggtttaa ggaaagcatt ctcggacgga acgcccccac catcaccttc
     2641 tcatttttcc accagttttc gtggttggca gctccgttga tgctgccctc cccgtaaagg
     2701 gtcacatccg agacatcgat tccggagata attcccgcaa acatcggcag cggattcccc
     2761 tcccaggttc cgaggttgta atcgcctgtc tcgtcatagc tctcgatcag tcccggcaga
     2821 attgcatagt gcgaccggtc ggtgtccgcc aaaagttccg cgcctttcgc cagttcaacg
     2881 ctgatcccac tctttaagaa cagactggta atcttatagg ttcctgccgg aatcagcacc
     2941 cggctctccg gcgggcacgc catgatcgct gccagtataa acgtggtgtc gtccgacaca
     3001 ccgtctccct tggcaccgaa tatgtctaca ccag
//
LOCUS       DBMDQJ010000269         3033 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11662_length_3033_cov_1.585607, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000269 DBMDQJ010000000
VERSION     DBMDQJ010000269.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11662_length_3033_cov_1.585607"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="ACZO9R_06375"
     CDS             <1..1203
                     /locus_tag="ACZO9R_06375"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR0912726.1"
                     /translation="RQRMEEEQWDLCRLLDAALHGRPAEMLPPAEKYGAILELADRHR
                     VLPLLYDVLAPKLPEEDENRKRLEKRSAETVRQSYRLLFLGKYVTGLLREHGIETVLL
                     KGSGTAGLYPVPELRKSGDIDLLVADQKAGEKAYEVLHEHGFRKASEQHAHHHIVCVG
                     TEQIGIEIHVALAEPFDSKSVNEWLAAHQAEFLAHCERTAVMGVEFPVLQPAYHAFYL
                     LLHMLQHFLRAGFGLKLLCDWVVFWEKGYGSGVEERFFSMAEECRVMGFARLVTAVCV
                     KYLGLSGEKVPLLLCRFDWEAEAANLEAFLTEILEAEEFGKSSADRMVALRGTGWRDY
                     CREFHHQMLLTYPGAGRYRILHPVLWVMMFCGFAYRNVKMRRVSGYAVLKKARARGKL
                     VEEMKLFR"
     gene            1240..>3033
                     /locus_tag="ACZO9R_06380"
     CDS             1240..>3033
                     /locus_tag="ACZO9R_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912727.1"
                     /translation="MDTWYWALEYVRVFFAYVMILFVWPSVVFRDYLRGRSRTVRFAF
                     CVTVPVVLLNTVVLSLGVFHILYGWLIAVLFYGTLLLGLLRWHPVRREQIKKISRLFL
                     GTYGIRLLLLRLRNRVKDGIGHVHAQFRQSIRGRRCVYLMLGVVVCFGMVYFSYGAFH
                     DYSYGFGDMYRHHSWIYGLLNGTPFYEGIYPEAMHCFIYAMRVLFGVKIYSSQLFLAG
                     IHVAVFLVSAYLLLKELFAWDGTAVLALALFLTVDLLCIDEIFSMSRLQWTLPQEFGL
                     YTQFLCALFLLRCLKTDFSDRSGSRRERIRKFLTDENLLLFLLSLAASLAIHFYVTMM
                     AFFLCVVIAACRLPALFQKRRFVSLVKAVCLGVLIAVLPMGIAYAKGVPFQGSIGWAV
                     NVINGMDTAEGRTSQAEQILEQTQTSSEQTSKEQTSSGERMTESGETTETAVQPTEQS
                     HGGNGQIPDGGIADAGQNSAPKQSAGNHMLAMAERMAASFAGKAQLVWKYGYVQLYRE
                     NRAEWIVFFSGLALILWAVYRMAAGIIAGLLKKGRDSKKIQQLFDGYPVLVGISVLLM
                     IVYAAPFLGLPELIAGSRLCSTEQLFILAVVV"
BASE COUNT          635 a          683 c          993 g          722 t
ORIGIN      
        1 agacagcgga tggaagaaga acaatgggat ttgtgcaggc tgctggatgc ggcgctgcac
       61 ggcagaccgg cggaaatgct cccgccggct gaaaaatacg gagccattct tgaactggca
      121 gaccggcatc gcgtgctgcc gctgctgtac gacgtgctgg cacccaagct gcccgaggag
      181 gatgagaacc ggaagcgcct tgagaagaga agcgcagaga cggtgcggca gagctaccgc
      241 ctgctgttcc tcgggaaata tgtgacagga cttttgcggg aacatggaat cgagacggtt
      301 cttttaaagg ggagcggcac ggcgggactc tatccggtgc cggaacttcg caagtccggg
      361 gatattgatc tgctcgttgc ggatcagaaa gcgggagaaa aggcgtatga ggtgctgcat
      421 gagcatggat tccggaaagc ctcagagcag cacgcgcacc atcatatcgt ctgcgttggc
      481 acagagcaga tcggaataga aattcacgtt gcgctggcag agccgttcga ctccaaaagc
      541 gtcaacgagt ggctcgccgc acatcaggcg gaatttctgg cgcactgtga gaggacggcg
      601 gtgatggggg tggagtttcc ggtgctgcag ccggcatatc atgcgtttta cctgctgctt
      661 catatgctgc agcatttcct gcgtgccgga ttcgggttaa agctgctctg cgactgggtc
      721 gtattctggg aaaagggata cggcagcggg gtagaggagc gttttttctc catggcagag
      781 gaatgccgcg tgatgggatt tgcgcggctg gtgacggcgg tctgtgtaaa atatctggga
      841 ctttctgggg aaaaagtgcc cctgctgctt tgccgatttg actgggaggc ggaagctgca
      901 aatctggaag cattcctgac agagattctg gaagcggagg agttcggcaa gtcaagtgcc
      961 gaccgtatgg tggcgctgcg gggaaccggc tggcgggatt attgcaggga atttcatcat
     1021 cagatgctgc tcacctatcc aggggcaggg cgctaccgca ttctgcatcc ggtgttgtgg
     1081 gtgatgatgt tctgcggttt tgcataccgc aacgttaaga tgcgccgggt atcggggtac
     1141 gcggtgttaa agaaggcgag agcgcgcgga aagcttgtgg aggagatgaa gctgttccgc
     1201 tagaaaaaag gatgttcgac gggaaaggag gagagcaaag tggatacatg gtactgggca
     1261 ctggaatatg tcagggtatt ttttgcctat gtgatgattc tgtttgtgtg gccgtctgtg
     1321 gtattccggg attatttaag aggcaggagc cgcacggtgc ggtttgcttt ctgtgtgacg
     1381 gttccggttg tgctgctcaa tacggtggta ctaagtctgg gcgtgtttca tatactgtac
     1441 ggatggctga ttgccgtgct gttttacggc acattgctgc ttggcttgct gagatggcac
     1501 cctgtgcggc gggagcagat caaaaagatc tcccgcctgt ttctgggaac ctacggcatc
     1561 cggcttttgc tgcttcgcct gcgcaacaga gtaaaagacg ggatcgggca tgtacatgca
     1621 caattcaggc agagcatacg gggacggcgc tgcgtctatc tgatgctggg ggtggttgtg
     1681 tgctttggca tggtgtattt ttcctacggg gcattccatg attactccta cgggtttggc
     1741 gatatgtacc gccaccactc gtggatctac gggctgttaa acggtacgcc tttttatgag
     1801 ggaatctacc cggaagccat gcactgcttt atctacgcca tgcgcgtgct gttcggggta
     1861 aagatttaca gcagccagct gtttctggcg ggaatccacg tcgcggtttt tctggtcagc
     1921 gcgtatctgc tgctgaagga attatttgca tgggatggaa ccgcggtgct tgcactggcg
     1981 ttgttcctga ccgtggatct tttgtgcatt gacgagatct tttccatgtc ccgtctgcag
     2041 tggacgctgc cgcaggagtt cggactgtat acacaattcc tgtgcgcact gtttctgctt
     2101 cgctgcctta agacggactt tagcgacaga tccggcagcc ggagagagcg gatcagaaag
     2161 tttttgacgg atgagaatct gctgctgttt ctgctgtcac tggcggcttc ccttgccatt
     2221 catttttatg tcacgatgat ggcgtttttc ctctgcgtgg tgattgccgc gtgcaggctt
     2281 cccgctcttt ttcaaaagag aaggtttgta tctctcgtaa aggcggtctg cctcggtgtg
     2341 ctgatagcgg tgctgccgat gggaattgcg tatgcgaagg gtgtaccgtt tcagggatcg
     2401 atcggatggg cggtcaatgt cataaacggt atggataccg cggaggggcg taccagccag
     2461 gcggaacaga tcttagaaca gacgcagaca tcgagtgagc agacatcgaa ggagcagaca
     2521 tcgtccggtg agaggatgac agagagcggg gagacgacag aaacggcggt gcagccgacg
     2581 gaacaatccc acggggggaa cgggcagata ccagatggcg ggattgcgga tgcgggacaa
     2641 aatagcgctc cgaaacaatc cgccggtaat catatgcttg cgatggcaga acggatggcg
     2701 gcatcgttcg ccggcaaggc acaactggtc tggaaatacg ggtatgtgca gctgtaccgt
     2761 gaaaaccggg cggaatggat tgtgtttttt agcggtctgg cactgatcct ctgggcggtg
     2821 taccggatgg cggcggggat tatcgccggc ctgctaaaaa agggaagaga cagcaaaaag
     2881 atccagcagc tgtttgacgg atatccggtg ctggtcggaa tatcggtatt gcttatgatt
     2941 gtctatgcgg cgccgttcct cgggctgccg gagcttattg caggctccag gctatgctct
     3001 acggaacagc ttttcattct ggcggtcgtg gtc
//
LOCUS       DBMDQJ010000270         3022 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11710_length_3022_cov_1.520712, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000270 DBMDQJ010000000
VERSION     DBMDQJ010000270.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11710_length_3022_cov_1.520712"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(302..1021)
                     /locus_tag="ACZO9R_06385"
     CDS             complement(302..1021)
                     /locus_tag="ACZO9R_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912728.1"
                     /translation="MRGHWYKTIVTLTMVLMLAGCGGNGAEAEQAAQTEQVEQTEQTL
                     EQVLEEHFLAAEDAEDVATVVSQYAEETPDPALAEFLISYYQIPDEEQSKTRYYYNHI
                     DLNEDGTDEIFVVVVGEYTKVSFGDPALILSEQADGTFQVIESFEGIHTPVTVSERMT
                     NGWHDIIYQEYGRGAEDGYRICHYNPNGGYQTDLSEVVDEVEPQDGTQIISNNFIDDM
                     DKGNYMTLAPEEDGGSEAQGN"
     gene            complement(1256..1519)
                     /gene="rpsR"
                     /locus_tag="ACZO9R_06390"
     CDS             complement(1256..1519)
                     /gene="rpsR"
                     /locus_tag="ACZO9R_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR0912729.1"
                     /translation="MAFNKAADKEGAPMKRRPMHRRKKVCVFCGKDNVIDYKDTNKLK
                     RYISERGKILPRRITGNCAKHQRALTVAIKRARHVALMPYVCE"
     gene            complement(1548..1994)
                     /locus_tag="ACZO9R_06395"
     CDS             complement(1548..1994)
                     /locus_tag="ACZO9R_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR0912730.1"
                     /translation="MNKVILMGRLTRDAEVRYSQGENSTAIARFSLAVDRRFRRDGDE
                     QTADFINCVAFGRTAEFLERFGRKGTKFVLEGRIQTGSYTNKDGQRVYTTDVVAENVE
                     FAESKNASGGGDNSGFTPSDRPSPSSAAGDGFMNIPDGIDEELPFN"
     gene            complement(2045..2335)
                     /gene="rpsF"
                     /locus_tag="ACZO9R_06400"
     CDS             complement(2045..2335)
                     /gene="rpsF"
                     /locus_tag="ACZO9R_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR0912731.1"
                     /translation="MNKYELALVVSAKIEDDARTATVEKAKEYITRAGGTVTEVEDWG
                     KKKLAYEVQKMSEAYYYFIQFDAEPTVPAEVEQDVRIMDNVLRFLVVRKDEK"
     gene            complement(2481..2681)
                     /locus_tag="ACZO9R_06405"
     CDS             complement(2481..2681)
                     /locus_tag="ACZO9R_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HIR0912732.1"
                     /translation="MNQYHYEVGDIVKLKKQHPCGSSEWEILRVGADFRLKCVGCGHQ
                     IMIARKLVEKNTRGLRKAGETE"
     gene            complement(2767..>3022)
                     /locus_tag="ACZO9R_06410"
     CDS             complement(2767..>3022)
                     /locus_tag="ACZO9R_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912733.1"
                     /translation="TTSWVILSLHLPWAFRWEPVFYISHADMLIIGGTSLAVYPAAGL
                     IDYYRGNKLVLINKSTTPMDARADLLIQAGLGDVFGQIEV"
BASE COUNT          718 a          864 c          564 g          876 t
ORIGIN      
        1 aaatccagaa aaaagcactc ctgaagaagt gtcttccata tacacaaaca cattgatgat
       61 aatcagaagc attgtaagca gcagcggcag atacaaatat gtacgaagct ttcctttaat
      121 ctttacgtta ctttttctca caagcaagcc tccatcattc tatacaaaaa cactatcata
      181 ccgtgcatta taccattttg accgtgcaat ataaagcctt ttttgcccga aaaaagactg
      241 cccacaacaa gttggacagt ccaaaatttc agccgctata tctagttggt ttgcgcttta
      301 tctagttgcc ttgcgcttcg cttccgccat cttcctccgg tgcgagtgtc atataattcc
      361 ccttatccat atcgtcgata aagttgttgg agatgatctg cgtaccgtcc tgcggctcca
      421 cctcatccac aacctcactc agatctgtct ggtatcctcc attcggatta taatggcaga
      481 tacggtatcc atcttccgct ccacgaccgt attcctggta aataatatca tgccagccgt
      541 tcgtcatgcg ctcactgact gtcaccggcg tgtggattcc ctcaaacgac tcgatcacct
      601 ggaacgttcc gtccgcctgt tccgataaga tcagcgccgg atcaccaaac gatacctttg
      661 tatactcacc gaccacgaca acgaaaatct catccgtccc atcctcattc agatcgatgt
      721 gattatagta ataccgggtt ttgctctgct cttcgtccgg aatctgataa taagagatca
      781 gaaactccgc cagtgccgga tccggcgtct cctccgcgta ctgcgagacc actgtcgcca
      841 catcctccgc atcctccgcc gcaaggaaat gctcttccag cacctgctca agcgtctgct
      901 ccgtctgttc cacctgctcg gtctgcgccg cctgctctgc ctctgcgcca ttcccgccgc
      961 atcctgccag catcagaacc attgtcagcg tgactatcgt tttgtaccag tgtcctctca
     1021 ttcgcgttcc cactcctctg cacctcttgc acttacacat acccataata ttatatgatt
     1081 cttgtctcat ttccaagccc atttttctat tcttaaggaa agttttagaa tcagtggcat
     1141 tacgaaaatg caatcaccta aaaaaagacc agcaatcgga aggattgttg gtcttttact
     1201 tatcagctta atcgctgcga ttatgcgaag cttattcgct gcgattaagt aaaacttatt
     1261 cgcatacgta cggcatcaat gccacgtgac gtgctctctt gatcgctaca gtgagcgctc
     1321 tctgatgctt tgcacagttg cctgtgatac ggcgaggaag gatctttcct ctctcagaga
     1381 tgtatctctt taacttgttg gtatccttgt aatcgatcac attgtcctta ccacagaata
     1441 cacaaacttt ttttcttcta tgcatcgggc gtctcttcat tggagcgcct tctttgtctg
     1501 ctgccttatt gaaagccatg ataattacct cctgttatta tcgtccatta gttaaatggt
     1561 aactcttcat caattccgtc cggaatattc atgaagccat cacccgctgc actgctcggt
     1621 gacggtctgt cggaaggggt aaaaccggaa ttatctccgc cgccggaagc attcttgctc
     1681 tctgcgaact ctacattctc agcaacaacg tccgtcgtgt atacacgctg tccgtccttg
     1741 ttggtgtagc ttccggtctg aatacgtccc tccagaacga atttagttcc cttacgtccg
     1801 aatctctcca gaaactctgc agttctgcca aatgcaacgc agttgataaa atctgctgtc
     1861 tgctcgtccc cgtcacgacg gaaacggcgg tctaccgcaa gcgaaaatct tgcgatggca
     1921 gtggagttct caccctgtga gtaacgcacc tctgcatctc tggtcagtct tcccataaga
     1981 atcactttgt tcatatacga ttcctctctt ccctcaaaca aacgttatca gatcaaagaa
     2041 tctcttattt ctcgtctttt ctcacaacta agaaacgaag tacgttgtcc atgatacgaa
     2101 catcctgctc aacttctgct ggaacagtcg gctctgcatc gaactgaatg aagtagtaat
     2161 aagcttcgct catcttctga acttcgtaag ctaacttctt cttaccccag tcctctactt
     2221 ctgttacagt accgccggca cgagtgatgt actcttttgc cttctctact gtagctgttc
     2281 tcgcatcgtc ttcgatcttt gcactgacaa cgagtgctaa ctcatactta ttcatgcgaa
     2341 tttcctcctt ttggtctctg gcccacgttt tcattacatg agcaaggaat aattattata
     2401 tcacgagcat ctactataac acgttttccc tgtaattaca agtgctttct tgaaaaaagc
     2461 gcaaaaactg cgcagcgtgc ctattctgtc tcccccgcct tgcgcagtcc tctggtattt
     2521 ttctccacaa gctttcttgc aatcatgatc tgatgcccgc agcccacaca cttcaggcgg
     2581 aaatccgcgc cgacgcgcag gatctcccac tcactgctgc cacacggatg ctgctttttc
     2641 agctttacga tgtctcccac ttcataatgg tactggttca tggtcttcct cactttcctg
     2701 atgtagtgat agtgtagcat gtttcactcc gctgcggtag aaaaaattca tttgctccac
     2761 gtccgtctac acctcaatct gcccgaacac atcgccgagt cccgcctgaa tcagcagatc
     2821 tgccctcgcg tccatcggcg tcgtcgattt gttgattaac accagtttgt ttccccggta
     2881 ataatcgatc agaccggctg ccggatatac cgcaagcgat gttccaccga taatcagcat
     2941 atccgcatgg ctgatataaa ataccggctc ccatcgaaag gcccatggca aatgaagcga
     3001 tagaattacc cagctggttg ta
//
LOCUS       DBMDQJ010000271         3013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11746_length_3013_cov_1.748283, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000271 DBMDQJ010000000
VERSION     DBMDQJ010000271.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11746_length_3013_cov_1.748283"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..786)
                     /locus_tag="ACZO9R_06415"
     CDS             complement(<1..786)
                     /locus_tag="ACZO9R_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912734.1"
                     /translation="MKSTFSDSPDNHNRWFRPHFSHFCATRLGRFINESRYGGILYLA
                     LWLVVLGLVFTLFQTIFLSIAYTGRLTPDDYEVLHVEQTEYDQSIGAYDSGIIRRFSD
                     YLVTVQYEVDGTRYTADLSLGQAFFTGNRVARAGADGSSVLGTIFYKSSDPKRLKYIP
                     ARRHILPFSTGKYVCREDTDDFAAQVCALLQQEDYGSLAALSYNGRDIPVTAEDTEEY
                     FHDDLVYFRKNVGDLSLLEGTTVYHDTPWATAVTIRYYSKEAQE"
     gene            949..1887
                     /locus_tag="ACZO9R_06420"
     CDS             949..1887
                     /locus_tag="ACZO9R_06420"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR0912735.1"
                     /translation="MKYYMAPMEGVTGYVYRNAYAKYYGGIVRYFTPFVANKKLGHRE
                     KNDVCPEHNAGMAVVPQILTNRAEDFLAIAEILREYGYDTVNLNLGCPSGTVAARGRG
                     AGFLAMPDELDHFLEEIFEKCPLRISIKTRIGKENPAEWERLLAIYEKYPLEELIVHP
                     RVQTDFYKNRVNLEAFARAEKNSRHSLCYNGDIVDAASAAHIREGFPSVDKFMIGRGL
                     IKHPWLLEELSGGRQEKQERKERFFRFHNAILEGYRQQMSGDKNTLFKMKELWAYWGE
                     NFADADRHLKKIRKCERAAEYECLVDALFREKELRV"
     gene            2074..2991
                     /locus_tag="ACZO9R_06425"
     CDS             2074..2991
                     /locus_tag="ACZO9R_06425"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HIR0912736.1"
                     /translation="MESYTLEEKVLRKVMRNIPMGLIVSKEGFRRKIYYVNDTACEIM
                     GYTREEYIQKMQDGWTDLMDVDLREVIREHNEQIRSGTPFEVLSRPQTKNGGYKWILS
                     RVNVQMQEGPLCYVSFMDVTDRMEREELCRQENEVLREQAMQDSFTKLLNRGALEKHV
                     CEALQERGGDEEDVFILLDVDNFKQINDIYGHGVGDMILMRMAGILNEVFGDHSCIGR
                     MGGDEFAIFLRDISGRREIEEKVQKLLEEVRAEKGRMHLRKEPTASVGIAFVPESGST
                     FVDVYRAADQALYHVKNSTKNGMAFCDFV"
BASE COUNT          837 a          603 c          893 g          680 t
ORIGIN      
        1 ctcctgtgcc tccttggagt aatagcggat ggtgactgcg gttgcccagg gggtgtcgtg
       61 atatacggta gttccctcca gcaacgaaag atcccccacg tttttacgga aatacaccag
      121 atcatcatga aaatattctt ctgtgtcttc cgcggttaca gggatatctc tgccgttata
      181 agaaagcgcg gcgagggagc cataatcctc ctgctgtaaa agcgcgcaga cctgtgcggc
      241 aaaatcatcg gtatcctccc ggcagacata tttgccggtt gaaaatggga gaatgtgccg
      301 tctggccgga atgtacttca ggcgttttgg atcggaactt ttatagaaaa tggtgccaag
      361 tacagacgag ccgtctgcac cggcgcgtgc cacgcggttt ccggtgaaaa atgcctgacc
      421 gagtgaaaga tctgcagtat agcgcgttcc atcaacctca tattgcacgg ttaccaggta
      481 atcggaaaaa cgtctgatga taccggaatc gtaggcaccg atactctggt catattcggt
      541 ctgctccaca tgaaggactt cgtaatcatc cggcgtgagc cggccggtat aggcgatact
      601 taaaaagatg gtctgaaaaa gagtaaaaac gagcccgagt acaaccagcc acagggcgag
      661 atagagaata ccgccatatc tggattcgtt gatgaaacgc ccgagacggg tcgcacaaaa
      721 atgcgaaaaa tgggggcgga accatcggtt gtgattgtcc gggctgtctg aaaatgtcga
      781 cttcatagaa attctcctga atataatgag ctataaacgg gtttgtgaaa gctgtatcaa
      841 tagcagtata gcaaaaaaag gcggaatttt ccactatttg acaggcggga agagctgtat
      901 taagataagg gacagatttc tgacagaggg agaagacagt tggaacagat aaagtattat
      961 atggcaccca tggaaggtgt caccggatat gtctaccgga atgcatacgc aaaatattac
     1021 ggcgggatcg tacgctattt tacgccgttt gtggcgaata aaaagcttgg acaccgggag
     1081 aaaaatgacg tctgcccgga acacaatgcg ggaatggcgg tcgttccgca gatacttacg
     1141 aaccgcgcag aagattttct ggcgatcgca gagattctgc gggagtatgg gtacgatacg
     1201 gtcaacctga acctcggctg cccgtcgggc acagtggcgg caagagggcg cggcgccgga
     1261 ttccttgcga tgccggacga gctggatcat tttctggaag agatttttga aaaatgtccg
     1321 ctccggatct ccatcaagac acggatcgga aaagagaacc ctgcagaatg ggagcggctg
     1381 cttgccatat atgaaaaata tccgttagaa gaactgatcg tacacccgag ggtgcagacc
     1441 gatttttata agaaccgggt caatctggag gcatttgcac gggcagagaa aaacagcaga
     1501 cattcgctct gttataatgg tgacattgtg gacgcagcat ccgcggcgca catccgggag
     1561 ggctttccgt cggtggataa gttcatgatc ggccggggac tgataaagca cccctggctt
     1621 ctggaggaac tttccggcgg cagacaggaa aagcaggagc gaaaagagcg gtttttccgg
     1681 tttcacaatg cgatcctgga gggataccgg cagcagatgt ccggggacaa aaatacctta
     1741 tttaagatga aggaactgtg ggcgtactgg ggagagaact ttgcggatgc ggaccggcat
     1801 ctaaaaaaaa tccgaaaatg tgagcgggct gctgagtatg aatgtctggt ggacgcgctg
     1861 tttcgtgaaa aagagctgcg cgtgtaggaa aatagtgggg tatgcggcaa tagatttgtg
     1921 tgatataaca aaaaagaagc tttccaatct gtaacattta tataaaaaaa cgaaagggac
     1981 tttctatttg ctgtaaaaag tattatactg tactccgtag tatgcttcat gagaacgata
     2041 ggggaattgg ggaagaggtt ttaaagaggg tatatggaaa gctatacgtt agaggagaaa
     2101 gtgcttcgca aggtcatgcg gaatattccg atgggactga ttgtgagcaa agagggattt
     2161 cggcgcaaga tttactatgt caatgacacg gcgtgtgaga ttatggggta cacgcgggaa
     2221 gagtatatcc aaaagatgca ggacggatgg acggatctga tggatgtcga tctgcgggag
     2281 gtcatccgcg agcacaatga acagattcgt tccgggacgc cgtttgaggt gctctcaaga
     2341 ccgcagacga agaacggcgg ttacaagtgg atattaagca gggtgaatgt gcagatgcag
     2401 gagggaccgc tctgctatgt cagctttatg gatgttaccg accgcatgga gcgggaggaa
     2461 ctgtgccggc aggagaacga ggtgctcagg gagcaggcga tgcaggactc ctttacgaag
     2521 cttctaaacc gcggcgcgct ggagaagcat gtctgcgagg cgcttcagga gagaggcggc
     2581 gatgaggagg atgtattcat tcttctggat gtcgataatt ttaaacagat caatgacatc
     2641 tacggtcacg gagtcgggga tatgattctg atgcggatgg cgggaatctt aaatgaagtg
     2701 ttcggagacc attcctgtat cggcaggatg ggcggtgatg aatttgctat tttcctcaga
     2761 gacatcagcg gccggagaga gattgaagag aaagttcaaa agcttttgga ggaggtacgc
     2821 gccgagaagg ggcggatgca tttgagaaag gaaccgaccg ccagcgttgg cattgcattt
     2881 gtgccagaat cgggaagcac gttcgtggat gtgtatcgtg cggcggatca ggctctgtat
     2941 cacgtgaaga attcgacgaa gaacggaatg gcgttctgtg attttgtata atttttccta
     3001 ttgaaaaaaa tca
//
LOCUS       DBMDQJ010000272         3009 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11765_length_3009_cov_1.456327, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000272 DBMDQJ010000000
VERSION     DBMDQJ010000272.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11765_length_3009_cov_1.456327"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(167..1315)
                     /locus_tag="ACZO9R_06430"
     CDS             complement(167..1315)
                     /locus_tag="ACZO9R_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4422 domain-containing protein"
                     /protein_id="HIR0912737.1"
                     /translation="MKIRIYVMTHKKFEMPQSPLFRPLHVGRACGEDLGYPGDDTGEN
                     ISDKNCYYSELTGLYWVWKNCHDVDYVGTCHYRRYLLGADERILMEDDYEKLLSEYDL
                     ITTKQVALNNSYYYGFCANHNKKALDAAGEVIKERYPAYYPAFERLVHGTRTYFGNMF
                     VTSKELYDSYCSWLFSIFAEVEKRICLETGEDAYHKRVFGFISEFLLLVWVTVQGLSV
                     CECKVGMIGEKAETREMKEQLAGYFARRDVDGAKAYFLERRKERPDVLMEASDVTGEL
                     RLCMQVIATAGMEQTRYGTNLLERENRFKELMQMFDRLDQIVYRYRNGLQKKEDAVFL
                     KEQGITDTALLIALRIPGDDAARQKELFAQITADKKALDGTTADTVTV"
     gene            1402..2709
                     /locus_tag="ACZO9R_06435"
     CDS             1402..2709
                     /locus_tag="ACZO9R_06435"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR0912738.1"
                     /translation="MPQPLIYHIDVNSAFLSWEAAELLRQDPDAPDIRSFPSVIGGSE
                     KTRHGIVLAKSPSAAALGVCTGEPLAQARRKCPDLKIYAPHYSIYVERSRQFMALLKE
                     YAPSVDAYSIDEAFCDMTGTSSLYGNPVDFAHRLKDEIHTRLGFTVNIGISSNRLLAK
                     TASDFEKPDKVHTLFPSEMEEKFWPLPIEDLFFVGKSTALRLRSLGITTIGQLAKSDH
                     AMILSVFKSHGDTIWNYANGIESTPLSHNDAASKGFGNSLTLQYDVTDAAVAKHVLLS
                     LCETVGARIRAAGAYVSVVQVQIVDNDFRHTSRQVTLASSTNVTEKLYTCACELFDEL
                     WDHRPIRLLGVSTGKATQEHYEQYDLFDMQKNEKLSRLNVAVDAIRSRYGEDSIKRAC
                     FIEADDAAPALSHMTGGMQRAKRNAAHPADAASDPARPRNRQP"
BASE COUNT          693 a          931 c          691 g          694 t
ORIGIN      
        1 aatcgaaccc gcattgccgg agtcggagtc cggtgttcta tccattagac tacggttgcc
       61 cattgcgcat ctctgaaaaa ctcatatcgc aacgaacata tactagcaca ttggcatttt
      121 gtttgtcaac gcttatccca aaagcttttc tgaaaattgt gtgaactcac acagtaacgg
      181 tatctgcggt ggtgccatcc agtgcttttt tgtctgccgt aatctgtgca aacagttcct
      241 tctggcgcgc tgcatcatcc cccgggatcc gcagtgcgat cagaagcgcg gtgtccgtga
      301 ttccctgttc ctttaaaaag acggcatcct cttttttctg cagaccgttg cggtagcggt
      361 acacgatctg gtccagacgg tcaaacatct gcatcagctc tttgaaacga ttttcgcgtt
      421 ctaaaaggtt cgtcccgtaa cgtgtctgct ccattcccgc cgttgcgatc acctgcatgc
      481 aaagccggag ttctcccgtc acatccgacg cctccatcaa aacgtccggg cgctcctttc
      541 tgcgctcaag gaaatacgcc ttggcgccgt ccacatcgcg tcttgcaaaa tagccggcaa
      601 gctgctcttt catctctctc gtctccgcct tctcaccgat catccccacc ttgcactcac
      661 acaccgaaag tccctgcacc gtcacccaga ccagcagcag gaattcggag ataaatccga
      721 acacgcgctt atggtaggca tcctcccccg tctccagaca gatccgtttt tccacctcgg
      781 caaatatgga aaagagccag ctgcagtacg aatcatagag ttccttcgac gtgacaaaca
      841 tattcccgaa atacgtcctt gtgccatgca ccagcctctc aaacgccgga taatatgccg
      901 gataacgttc ttttatcacc tctcccgccg catccagtgc ctttttgtta tgattggcgc
      961 aaaaaccgta atagtaggag ttattgagtg caacctgctt cgtcgtaatc agatcgtact
     1021 ctgataaaag cttttcgtaa tcgtcctcca taagaatcct ctcatccgcc ccgagcagat
     1081 atctccggta atgacaggtg cccacataat ccacatcgtg acagtttttc cacacccagt
     1141 agagcccggt cagctcactg taatagcaat ttttgtcgga aatattctct ccggtatcat
     1201 cccccggata gcccaggtct tccccgcacg cgcgtcccac atggagcgga cgaaacagcg
     1261 ggctctgcgg catctcaaac tttttatgtg tcatgacata gatacggatc ttcattcttc
     1321 ctccaggaac atatgtttgt attttcattt acttttgttc acttctctgc tataatacac
     1381 catcataagg aggattacca tatgccgcag ccgctcattt atcacattga cgtcaattcc
     1441 gcatttttaa gctgggaagc cgccgaactt ctgcgccagg atccggacgc cccggacatc
     1501 cgctcttttc cttccgtcat cggcggcagc gaaaagacac ggcacgggat tgttctcgcc
     1561 aagtcgccct ccgcagccgc gctcggcgtc tgtaccggcg agccgctcgc ccaggcaagg
     1621 cgcaagtgcc ctgatttaaa aatctatgcc ccgcactaca gcatctatgt ggagcgttcc
     1681 cgccagttca tggcacttct caaagaatat gcgccctcgg tggacgccta cagcatcgac
     1741 gaggccttct gcgacatgac aggcacctcc tcgctctacg gcaatccggt ggactttgcc
     1801 cacaggctga aggacgagat tcacacccgg ctcggcttta ccgtgaacat cggcatctcc
     1861 tccaaccggc tgctcgccaa gactgcctcc gactttgaaa aaccggacaa agtacacacg
     1921 cttttcccat ccgaaatgga agaaaagttc tggccgcttc cgatcgagga tctgtttttc
     1981 gtcgggaaat ccaccgccct aaggctccgc tccctcggca tcacaaccat cggccagctg
     2041 gccaaaagcg atcacgccat gattctctcc gtctttaaat ctcacggcga caccatctgg
     2101 aattatgcca acggaatcga atccacaccg ctgtcccaca acgacgccgc aagcaaggga
     2161 ttcggcaatt ccctcacgct ccagtacgat gtgacggatg ccgcggtggc aaagcatgtg
     2221 cttctctcgc tctgtgagac ggtcggtgcc agaatccgcg ctgccggcgc ctatgtcagc
     2281 gtggtgcagg tgcagattgt ggacaatgat ttccgccaca cttccagaca ggtaacgctt
     2341 gcttcctcca ccaacgtcac ggagaagctg tacacctgtg cctgtgagct cttcgacgag
     2401 ctctgggatc accgtccaat ccggctgctc ggcgtctcga ccggcaaggc gacgcaggaa
     2461 cactacgagc agtacgacct gtttgacatg cagaagaacg agaagctctc ccgcctgaat
     2521 gtggcggtcg acgccatccg ctcccgctac ggggaagatt ccatcaagcg cgcctgcttt
     2581 atcgaagcgg acgatgcagc gcccgcgctc tcccacatga ccggcggcat gcagcgcgcc
     2641 aagcgcaatg cagcgcatcc ggcagacgcg gcgtccgatc cggcacgtcc ccgtaatcgc
     2701 cagccgtaac ggaaaaggat ggggataagg aatattctga aaaaccgata ccggaaagtt
     2761 ccggatagaa cagacggcgg caaattcttt gaagcaaaat tcgtgtattt cttccatgcc
     2821 acgtccggca tcccatttcg tgtcttggcc acgtttggac actcacggag tgtgtccgac
     2881 aagctgcaga caatgagcgg actttctggt gtccctaatg gcacctcttc acagacaata
     2941 taaaaccgcg cgaaccgaaa ctcgccggca tctgctattg aaaataaggc agatgccgac
     3001 tcagacatc
//
LOCUS       DBMDQJ010000273         2990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11839_length_2990_cov_1.107996, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000273 DBMDQJ010000000
VERSION     DBMDQJ010000273.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11839_length_2990_cov_1.107996"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /gene="fliI"
                     /locus_tag="ACZO9R_06440"
     CDS             <1..230
                     /gene="fliI"
                     /locus_tag="ACZO9R_06440"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560451.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HIR0912739.1"
                     /translation="TSEHKELAGRLKNVLATYNEAEDLINIGAYKSGSNREIDYAVQK
                     INAVNQFLCQRTDEKFLFDEEIDLLKKLFED"
     gene            260..700
                     /gene="fliJ"
                     /locus_tag="ACZO9R_06445"
     CDS             260..700
                     /gene="fliJ"
                     /locus_tag="ACZO9R_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HIR0912740.1"
                     /translation="MAKFTYRMQNILDIKIKLENQAKIAYATASRQLAEEQEKLREIL
                     MRRAGYERKSKELVNGTLDIQEIRECKRAIDVMKSLQRAQMMKVHAAEKTLEMARIEL
                     NRVMVERKTHEKLRERAFEEFKQEIAQAESKEIDELVSYNYHEG"
     gene            716..1579
                     /locus_tag="ACZO9R_06450"
     CDS             716..1579
                     /locus_tag="ACZO9R_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotE family protein"
                     /protein_id="HIR0912741.1"
                     /translation="MAEEEKDEVLDKKAAKAQEKAARAEAKAQKKADRKKAKELNDDL
                     DMDDETSTGGKIAVFFVTLIIILIWLAIIVLLIKCDVGGFGSSVLAPVLKDVPYVNKI
                     LPDSATEEVSTEDDAYGYTSIDDAVARIKELEKELADAQNTSSANAEYIAQLESQSAE
                     LAQYKQNEADFEAEKQKFYQEVVFSDSAPDIEEYKQYYESIDPADAEIIYRQVVEQTA
                     ANDEMDDYVKAYSSMKPKEAAAIFDTMTDNLQLVADILDSMDAQSRANILGKMDAATA
                     AKVTAIMEPVE"
     gene            1665..>2990
                     /locus_tag="ACZO9R_06455"
     CDS             1665..>2990
                     /locus_tag="ACZO9R_06455"
                     /inference="COORDINATES: protein motif:HMM:NF014207.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HIR0912742.1"
                     /translation="MGDNNVASIGKMLMQTQSGQSVTGVDGADIGTTFRKMVDQMTQI
                     PGSSVSTGSQTAQNMQQLTPHSPEQNYDRYQYQKNKIPEQSTKDWTAGSQVSEKLESF
                     EESVRDVIKDELQVTDEQITEAMETLGLTVADLMNPQQLAALAAELTGTEDMSALLCN
                     EAFMNVMQSVGTLTEDLLQELGVTAEELTQLLEDAGQADMNADLMQTVQTADAADARR
                     VYADTADGIRDTAEQPQTAAVAETEPRAVRSKDITPEETDAQAKMADAGTEEETVADV
                     AAGTGKEQAQTGSNSNSGQQDFASQAHAGVDVHTGPTLTETVAMQGANSAGEYAAQVD
                     IADVVRQIVTYTRVNYTANQETTMEMQLNPEHLGKIYLEITSKDGTVSAHLTAQNEVV
                     KEALESQIADLKQNMNQAGVKVDAVEVTVGGHEFEKNLEQNAKQEEQQAQ"
BASE COUNT          923 a          613 c          914 g          540 t
ORIGIN      
        1 cgaccagcga gcacaaggag cttgccggac gtttaaagaa tgtactggct acttataacg
       61 aagcggagga tctgatcaac atcggagctt acaagagcgg ttccaaccgt gagattgact
      121 atgcggttca gaagatcaac gcagtgaatc agttcctgtg ccagaggaca gatgaaaagt
      181 tcctgtttga cgaggaaatc gatttattga agaaactatt tgaagattaa gaacgctccg
      241 cggaaaagag gcgcagtgta tggcaaagtt tacataccgg atgcagaaca ttcttgacat
      301 caagatcaag ctggagaatc aggcaaaaat tgcatatgcg acggcgagca gacagcttgc
      361 ggaagagcag gagaagcttc gagagatcct gatgcgcagg gccggctacg agagaaaatc
      421 aaaagaactg gtcaacggaa cgctggatat ccaggagatc cgggaatgca agcgtgcgat
      481 cgatgtgatg aagagtctgc agagagcaca gatgatgaag gtgcacgccg cggaaaagac
      541 gctggagatg gcgcgaatcg aactgaaccg ggtgatggtg gagcgaaaga cgcatgagaa
      601 gctcagggag cgtgcgtttg aagaatttaa gcaggaaatc gcccaggcag agagcaagga
      661 aatagacgag cttgttagct acaattacca tgaaggatag gcacggaggt ctcaaatggc
      721 agaagaagag aaagacgaag tattagataa aaaggcagca aaggcacagg agaaggctgc
      781 acgtgcagaa gcgaaggcac agaagaaagc ggaccggaaa aaggcaaaag agttaaatga
      841 tgatctggat atggatgacg agacatcgac tggcggcaag attgccgtgt tctttgttac
      901 cctgatcatt attttaatct ggcttgccat catcgtcctg ttgatcaagt gcgatgtcgg
      961 cggtttcgga tcatccgttc tggcgccggt gttaaaggat gttccttatg tgaacaaaat
     1021 cctgccggac agcgcgactg aggaagtgtc aacggaggat gacgcttacg gctatacttc
     1081 cattgacgat gcggttgcca gaatcaagga actggagaaa gaacttgcgg atgcacagaa
     1141 tacgtccagc gcaaatgcgg aatatatcgc acagcttgag agccagtccg cagaactggc
     1201 acagtacaag cagaacgagg cggattttga ggctgagaag caaaagttct atcaggaggt
     1261 tgtgttttca gattcagcac ccgatataga ggaatataag cagtattatg agagcattga
     1321 tcctgcggat gcggagatca tttaccgtca ggttgtagaa cagacggcag ccaacgacga
     1381 gatggatgat tatgtgaaag cgtactcttc catgaagcca aaagaggcgg ctgcaatttt
     1441 tgataccatg acagataatc tgcagctggt ggcagatatc ttagacagca tggacgcaca
     1501 atcgagagcg aatattctgg gcaaaatgga cgcggctact gcagcaaagg tgaccgcgat
     1561 catggaacct gtagaataga gaaggacagt ctgcttttca taatggcaga gggattcctt
     1621 caaaaatcag gctgcaggtg cagctatggg aaaggaggaa gagaatggga gacaacaatg
     1681 ttgcaagcat tgggaaaatg ctgatgcaga cgcagagtgg acagagtgtc acaggcgttg
     1741 atggcgcgga tatcggcacc acattccgga agatggtcga tcagatgaca cagatcccgg
     1801 gaagcagcgt aagtacagga tctcagacag cacagaatat gcagcagctt acaccgcaca
     1861 gtccggagca gaattatgac cgttaccagt atcagaagaa taagataccg gagcaaagca
     1921 cgaaggactg gacagccggc agtcaggtgt cagagaagct ggaaagcttt gaagagagtg
     1981 tgagagacgt catcaaggat gaacttcagg tgacggatga gcagatcacg gaagcaatgg
     2041 agacattggg actgaccgtg gccgatctga tgaatccgca gcagcttgca gctctggcgg
     2101 cagaattaac cggaaccgaa gacatgagcg cacttctctg taatgaagcg tttatgaacg
     2161 tgatgcagtc ggtgggaaca ctgaccgaag atctcttgca ggaactggga gtcacggctg
     2221 aggaattgac gcagcttttg gaggatgccg gacaggcgga catgaatgca gatctgatgc
     2281 agacagtcca gacagcagat gccgcagatg cacggagggt ttacgccgat acggcggatg
     2341 gaatccgtga tacggcagag cagccgcaga cagcagccgt agcagagaca gagccccggg
     2401 cagttagatc aaaagacatc actccggaag agacggatgc gcaggctaag atggcagatg
     2461 ccggcacaga ggaagagaca gttgccgatg tagctgcagg gacgggcaag gaacaggcac
     2521 agaccggatc gaattccaat tccggacagc aggattttgc gtcgcaggca catgcgggcg
     2581 tggatgtaca taccggcccg acgctgacag agaccgtggc aatgcagggc gccaatagtg
     2641 cgggagaata tgccgcacag gtggacatcg cggatgtcgt gcgtcagatc gtgacctata
     2701 cgagagtgaa ttacacggcg aatcaggaaa caacgatgga gatgcagctc aatccggaac
     2761 atctgggcaa gatttatctg gagatcacat ccaaagacgg aacagtttcc gcacatctga
     2821 ccgcacagaa tgaagtggtc aaggaggcgc tggaaagcca gattgcagat ctgaagcaga
     2881 acatgaacca ggcgggcgtg aaggtggatg ccgtggaggt aaccgtcggc ggacatgagt
     2941 ttgagaagaa cctggagcag aatgcaaaac aggaagaaca gcaggcacag
//
LOCUS       DBMDQJ010000274         2988 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11847_length_2988_cov_1.275719, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000274 DBMDQJ010000000
VERSION     DBMDQJ010000274.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11847_length_2988_cov_1.275719"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="ACZO9R_06460"
     CDS             <1..198
                     /locus_tag="ACZO9R_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912743.1"
                     /translation="LYSVDELLAKSTLFSDFKAVKEGNVYCTGSNFFQESTGTCDFIE
                     DLHKVLVDETDENYRFLKHVR"
     gene            201..1211
                     /locus_tag="ACZO9R_06465"
     CDS             201..1211
                     /locus_tag="ACZO9R_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR0912744.1"
                     /translation="MKRYVIAFGAMILLLGGLFFFNIGSGSVKMTGSEIADILLRRFG
                     GTADETARQIVWEIRLPRICAAVILGGALSVAGFLLQSFFQNPIAGPYVLGISSGAKL
                     VVALTMIALLEMGRAISSLGMIVAAFAGSMLAMGFVLLISCRVKKMSMLVVCGIMIGY
                     ICSAITDFCVTFADDSNIVNLHNWSMGSLSGMTWSNVRMMTAVVAVTMGVTFFLAKPI
                     GAYQMGEAYARNMGVKIRLLRVLLILLSSLLSACVTAFAGPISFVGIAVPHLVKTAMK
                     TAKPLLLIPGCFLGGAIITLFCDGFARTVFAPTELSISSVTAVFLVPVVIVMMLRRNE
                     DR"
     gene            1226..2356
                     /locus_tag="ACZO9R_06470"
     CDS             1226..2356
                     /locus_tag="ACZO9R_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912745.1"
                     /translation="MEQRKKESGIRLEDVAAGYGRKALLNHISLHVSPGNVLTLIGPN
                     GAGKSTILKTVTRQLAELGGTIFLEGTAMSRMREEEVARTLAMVMTERLHPEFMTCRE
                     VVATGRYPYTGRLGILSAEDWKLVDDAMQLVHAGDTADRNFSQISDGQRQRVMLARAI
                     CQEPRILILDEPTSYLDMRYKIDILSSIRRLAAERNMAVVMSLHELDLALKVSDVIAC
                     VEGDHIGKTGTPEEVFSGDYIQKLYGVDAECFDPRTGAVYLRGNKSRPKIFVIGGAGS
                     GIPVYHALQRKNVPFAAGILMENDVEYQDACAAASRVVSVKPFFPVAEKEIAEARSWI
                     ETCETCVCCAEAFGPYNEANRALREYAKTLGKLIPVEAQQNL"
     gene            2479..>2988
                     /locus_tag="ACZO9R_06475"
     CDS             2479..>2988
                     /locus_tag="ACZO9R_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR0912746.1"
                     /translation="MERGILVVSFGTTYQETREKNIDHMVALVREQYPHDLVEEAYSS
                     STVRKVLRERDGIAKDDVRQALCRMRDAGVRRVIVFPTHIIDGIENHRMKQEVTDCAP
                     WFEDVRIADALLKTPEDYQRTAEALWKSVAAEAGSSPVIFMGHGSEHAADESYERLEC
                     VLAQVTENDV"
BASE COUNT          680 a          614 c          984 g          710 t
ORIGIN      
        1 ctttacagtg tcgatgaact tctggcaaaa agcacgctgt tttctgattt taaggcagta
       61 aaagagggaa atgtatactg taccggaagc aatttctttc aggaatcgac gggaacctgc
      121 gatttcattg aggatctgca caaggtgctt gtggatgaga cagacgagaa ttatcggttt
      181 ttaaaacacg tgaggtaaga atgaaacggt atgtgatagc ctttggcgcg atgatcctgc
      241 tgcttggcgg gttgtttttc tttaatatcg gttccggaag tgtcaagatg acgggaagtg
      301 agattgcgga cattctgctg cgccgctttg gaggcacggc agatgagacg gcgcgtcaga
      361 ttgtctggga gatccgcctt ccccggatct gtgcggcagt tattctgggg ggagcgctct
      421 ctgttgcggg atttctgtta cagtcatttt ttcagaatcc gattgcgggt ccctatgtgc
      481 ttggcatctc atccggggcg aagcttgtgg ttgcgctgac gatgattgcc cttttggaaa
      541 tgggcagggc gatcagttca ctcggcatga ttgtggcggc gtttgccgga tcgatgcttg
      601 cgatggggtt cgtgctctta atttcctgcc gggtaaaaaa aatgtccatg cttgtggtat
      661 gcggcattat gatcggctat atctgttcgg caatcacgga tttctgcgtg acgtttgcgg
      721 atgactccaa cattgtgaac cttcacaact ggtcgatggg cagtctctcc ggaatgacct
      781 ggtcgaatgt gcggatgatg acagcggtgg tggcagtcac gatgggagtg accttttttc
      841 tggcaaaacc gatcggtgcg tatcagatgg gcgaggcgta tgcgagaaac atgggcgtta
      901 agatccgtct gcttcgcgta ttgctgattc tgctgtcgag tctgctgtct gcctgcgtga
      961 cggcctttgc cggaccgatc tcctttgtgg gaattgcggt tccgcatctg gtgaagacgg
     1021 cgatgaagac ggcgaaaccg cttttgctga taccgggctg ttttctgggc ggggcaatta
     1081 tcacgctgtt ttgcgacggc tttgcgagga cggtgtttgc tccgacggag ctttcgatca
     1141 gttcggtgac agccgtattt ctggtgccgg tagtgattgt gatgatgctg cgcaggaatg
     1201 aggacaggta acggaggaac gtgtgatgga acagaggaaa aaagagtctg gaatcaggct
     1261 tgaggatgtg gcggcagggt acggcagaaa agctctgctt aaccatattt cgctgcatgt
     1321 aagtcccgga aacgtcctga cgctgatcgg accgaacggt gcaggcaaat ccacgatatt
     1381 aaaaaccgtg accagacagc ttgcagagct tggaggaacg atctttctgg aggggacagc
     1441 catgagccgg atgcgggaag aggaagtcgc gcggacgctt gcgatggtga tgacggagcg
     1501 gcttcatccg gagtttatga cctgccgcga ggtggttgcg accggcaggt atccgtatac
     1561 cggaaggctc gggattctct ccgcagaaga ctggaagctt gtggatgatg ccatgcagct
     1621 ggtgcatgcc ggggacactg cagaccggaa tttttcacag atcagtgacg gacagagaca
     1681 gcgggtgatg ttagcgcgcg ccatctgcca ggagccgcgg attctgattc tggacgaacc
     1741 gacttcctac ctggacatgc gctacaagat cgacatttta tccagtatcc gaagactcgc
     1801 ggcagaaaga aatatggcgg ttgtcatgtc gctccacgaa ctggatctgg cgttaaaggt
     1861 atcggatgtg atcgcctgtg tggagggaga ccacatcgga aaaaccggaa cgcctgagga
     1921 agtattttca ggcgattata ttcaaaaatt atacggggtg gatgcagagt gttttgatcc
     1981 gcggacaggc gccgtgtatc tgcgcggaaa taaaagccgg ccaaagatct ttgtgatcgg
     2041 cggcgcgggc agcggcatac cggtctatca tgctttacag cggaaaaatg tgccgtttgc
     2101 ggcgggaatt ctgatggaga atgatgtgga atatcaggat gcgtgtgccg cggcaagccg
     2161 ggtggtttcc gtgaagccgt ttttcccggt cgcagagaag gagatcgcgg aggcgagatc
     2221 gtggattgag acgtgcgaaa cgtgtgtctg ttgtgcggaa gctttcggac cttacaacga
     2281 agcgaaccgc gcgctgcgcg agtatgcaaa aacactcgga aagctgattc ctgtagaggc
     2341 acagcagaat ttgtagaaaa tatgaaattg tggttgaatg tgggggatgg tgtgtgaatt
     2401 ggggatagca ggtgcgtcgg aaaggtaaat gtatcgggaa gtgcatgtgc accggcggca
     2461 gagaaagaga ggtgcgcgat ggagcgtggc atattggtcg tgagctttgg aacgacttat
     2521 caggagacaa gagaaaagaa tatcgatcac atggtggcgc ttgtcagaga gcagtatcca
     2581 catgatctgg tggaggaggc gtattccagc agcactgtga ggaaggtgtt aagagagcgg
     2641 gacggcatcg cgaaagatga tgtgaggcag gcgctttgcc ggatgcgcga tgccggggtg
     2701 cggcgtgtga tcgtatttcc gacccacatc attgacggga ttgagaacca ccggatgaag
     2761 caggaggtaa cagactgtgc accgtggttt gaggacgtcc ggattgcgga tgcgctgctt
     2821 aagacaccgg aggattatca gagaacggcg gaggctctct ggaaatcggt ggcggcggag
     2881 gcgggatctt cgccggtgat ttttatgggt catggaagtg agcatgcggc ggatgagagc
     2941 tacgagcgtc tggaatgtgt gcttgcacag gtgacagaga acgacgtc
//
LOCUS       DBMDQJ010000275         2987 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11850_length_2987_cov_1.571726, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000275 DBMDQJ010000000
VERSION     DBMDQJ010000275.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11850_length_2987_cov_1.571726"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="ACZO9R_06480"
     CDS             <1..475
                     /locus_tag="ACZO9R_06480"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR0912747.1"
                     /translation="ENAELSPKKGLFGYAFAGRDLTGKSVEDMVYEAQRKVILEIAEK
                     ESCVIIGRNADFILKDREDVLNVFIHGDMPEKVQRICRLYHVNEGEAAKMMADIDKRR
                     MTNYRFYTEQKWGMAGNYTLSMNSSRLGYEMCEKMIMDCTKAGVAHSCICTSQVL"
     gene            complement(700..1473)
                     /locus_tag="ACZO9R_06485"
     CDS             complement(700..1473)
                     /locus_tag="ACZO9R_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR0912748.1"
                     /translation="MTLLFTILVTFFAGMGAGLGTGFAGMSAAAVISPMLITFLDVDP
                     YMAVGIALSSDVLASAVSAYVYGKNKNLDIRNGVIMMVSVLIFTVIGSYISSLVPSTT
                     MGNFSVFMTFLLGIKFIVKPVMTTKESMAQVSAKKRAIQSVVCGVIIGFICGFIGAGG
                     GMMMLLILTSVLGYELKTAVGTSVFIMTFTALTGAVSHFAIGGAPDWTIFLLCVVFTF
                     LWARIASVFANKATPKTLNRATGVILVVLGIVVLAFSLL"
     gene            complement(1475..1909)
                     /locus_tag="ACZO9R_06490"
     CDS             complement(1475..1909)
                     /locus_tag="ACZO9R_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0912749.1"
                     /translation="MNTGIEITMKLSAAYSAVCRPLCHELNLPQTAFDILMFLANNPE
                     YTTARDIVEVRKIKANLVSINVDKLVSEGYLRREVDTADRRKTHLLCTDKAGAVIEQG
                     RTLQNRFMDSLLAHIDDAARKQLTESIHQMEQNLDHLLEEEH"
     gene            2200..>2987
                     /locus_tag="ACZO9R_06495"
     CDS             2200..>2987
                     /locus_tag="ACZO9R_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0912750.1"
                     /translation="MKMKKEDREIFLKQIFWEVVGSAFIAGGIYNFAVQAGFPMTGFS
                     GISIILYQLFGVPIGFSTILLNIPVAILCYRLLGRHFFFSSLRCMLISSLMIDYVAPL
                     FPVYSGDRLLAALCTGVFAGLGYAVIYMQNSSTGGSDFIIMAVKNLKPYWSVGKISFV
                     TDVIIILLGGFLLRDVDGIIYGMIVSFLLAIVVDKLMYGINSGKMTLVVTEHGKEICD
                     VIDSCCGRGSTILQGQGGYRCDNKQVVMCVCNNKEMYLLQHAVK"
BASE COUNT          767 a          618 c          823 g          779 t
ORIGIN      
        1 ggaaaatgcg gagctctctc caaagaaagg tttgtttggc tatgcttttg ccggacgcga
       61 ccttaccgga aaatccgtgg aagatatggt gtacgaagcg cagagaaagg tgattctgga
      121 gatcgcagaa aaggagagct gcgtcattat aggaagaaat gcagatttta ttttgaagga
      181 cagagaggat gtgctgaatg tatttataca cggcgatatg ccggaaaaag tgcagcgcat
      241 ctgcagactg taccatgtca acgaaggcga ggcagctaaa atgatggcgg atattgataa
      301 gaggcgtatg acaaattatc gtttttacac ggagcagaaa tggggaatgg cgggcaatta
      361 tacactgtct atgaacagct ccaggctggg gtatgagatg tgtgagaaaa tgattatgga
      421 ttgtacaaaa gctggcgttg cgcactcttg tatttgtacg tctcaggttt tataatgccc
      481 tgcaggacga tatctttttg ttgttacacc ggcctatgca tggagaattc caagaattgc
      541 gagagactgg atgatcggca ccgaatttaa agatgtcagg caggtatggc tgtgaccggg
      601 agatttgcgg ggaaccgggc gctggcggcg gtgggaagct gccagcgtcc ggtcggcata
      661 tttgtaagca tattctggtt agctgctatt ttacagattt tacaacaagg aaaacgccag
      721 cacgacgatg ccaaggacta caagaatgac accggttgca cggtttaacg tctttggtgt
      781 ggccttattg gcgaatacgg atgcgattct cgcccagagg aacgtgaaca ccacgcacag
      841 gagaaagatt gtccagtcgg gagcgccgcc gatggcaaag tgcgatacgg ctccggtgag
      901 agcggtgaaa gtcatgataa atacgctcgt gccaaccgcg gtctttaact catagcccag
      961 cacggaggtt aaaattaaga gcatcatcat gccgcctccg gcaccgatga aaccgcagat
     1021 aaatccgatg atcacgccgc agacaaccga ctggatggca cgtttttttg cggagacctg
     1081 cgccatggat tcttttgtcg tcatgaccgg ctttacaatg aatttgatgc cgagaagaaa
     1141 cgtcatgaag acggaaaaat tccccatggt ggtggacggg acaaggcttg agatgtagct
     1201 tccgatgacg gtgaagatta agacgctcac catcataatc actccgttgc ggatatccag
     1261 atttttgttt ttaccataga cgtaggcgga gacggcgctt gcgagcacgt cggaggagag
     1321 ggcgatgccg actgccatat atgggtcaac atccagaaaa gtgattaaca tcgggctgat
     1381 gacggcggct gcgctcattc cggcgaagcc ggtgccgagt ccggcgccca tgccggcaaa
     1441 aaaggtgacg agtatcgtga atagtaatgt catatcagtg ttcctcctct aacaggtgat
     1501 ccagattttg ctccatctga tggatgcttt ctgtgagttg ttttctggca gcatcatcga
     1561 tatgcgccag aagactgtcc ataaagcggt tctgaagggt gcgcccctgt tcgatgacag
     1621 cgccggcttt gtctgtgcag agcagatggg tctttcggcg gtcggctgtg tccacctcac
     1681 gccgcagata accttcgctg acgagcttgt ctacgttgat cgatacgagg ttggctttga
     1741 tcttgcggac ttccacaata tcgcgtgcgg tggtatattc cgggttgttc gccagaaaca
     1801 tcagaatatc gaacgcggtc tgcgggagat tcagttcgtg gcacaaaggt ctgcatacag
     1861 cactgtaggc agccgatagc ttcatggtaa tttcaatacc ggtattcata agtaaatgct
     1921 cctttcaatg ggataaaata atacttcacg tatgcaatat atatatggaa aggtctcgca
     1981 tacatatact tcatgtacga atacttcaca tatgaatggt tcatatatga agcatattaa
     2041 cacgacacat ccggattgta aagagaaaca tatttgacaa attccggatt ccggtgttac
     2101 aataggatac ggaaaagagc gttagcatat tatattcgta taatctgctg gtgctctttt
     2161 tttgtgttgt ggagacactc gcatgcaaag gaaggatgaa tgaaaatgaa aaaagaagat
     2221 agagagatat ttttaaaaca gatattctgg gaggttgtgg gaagtgcgtt tatcgctggc
     2281 ggtatttaca attttgcggt gcaggcggga ttcccgatga ccggattttc aggtatctcg
     2341 atcattttat accagctgtt cggcgtgccg atcggatttt ccacgatact gctgaatata
     2401 ccggtggcaa tattgtgcta tcgtctgctg ggaagacact ttttcttcag ctcactgcgc
     2461 tgtatgctga tttcttccct gatgattgac tatgtcgcgc cactgttccc ggtttactcg
     2521 ggagaccgtc tgcttgcggc actctgtacc ggtgtgtttg ccggactggg atatgcggtg
     2581 atttacatgc agaattcctc gaccggcggt tccgacttta ttattatggc ggtcaagaat
     2641 ctgaagccgt actggtctgt cgggaaaatt tcgttcgtga cggatgtgat catcattctt
     2701 ctgggcggat ttctgcttcg ggatgtggat ggaattatct acggaatgat tgtgagtttt
     2761 ctgctggcaa tcgtggtcga caagctgatg tacggcatca attccgggaa aatgacactt
     2821 gtcgtgacag agcatggaaa ggagatctgc gatgtgatcg attcctgctg cggccgcggc
     2881 tctacaatat tacagggaca gggcggttac cgctgtgaca acaaacaggt cgtgatgtgc
     2941 gtgtgcaaca acaaagagat gtatctgctt cagcatgctg ttaagga
//
LOCUS       DBMDQJ010000276         2978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11889_length_2978_cov_1.826555, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000276 DBMDQJ010000000
VERSION     DBMDQJ010000276.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11889_length_2978_cov_1.826555"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(311..>2978)
                     /gene="carB"
                     /locus_tag="ACZO9R_06500"
     CDS             complement(311..>2978)
                     /gene="carB"
                     /locus_tag="ACZO9R_06500"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR0912751.1"
                     /translation="IAHDETELIEILTNGLRLSRVGEVLVERCIAGWKEIEYEVMRDA
                     NGNCITVCNMENIDPVGVHTGDSIVVAPSQTLGDKEYQMLRTSALNIISELNITGGCN
                     VQYALNPESFEYCVIEVNPRVSRSSALASKATGYPIAKVAAKIALGYTLDEIKNAITG
                     KTYASFEPMLDYCVVKIPRLPFDKFITAKRTLTTQMKATGEVMSICNNFEGALMKAIR
                     SLEQHVDSLMSYDFTGLSREELVKQLHVVDDRRIWVIAEALRRGMDYDVIHDITKIDK
                     WFIDKLAILVEMEQKLKSEPLTVELLKEAKRIEFPDKVIAELTGKTETEIHDMRYANG
                     IVAAYKMVDTCAAEFEATTPYYYSVFGSENEAEETHPNKKVLVLGSGPIRIGQGIEFD
                     YCSVHCTWAFAKEGWETVIVNNNPETVSTDFDIADKLYFEPLTPEDVEAIVKLEKPDG
                     AVVQFGGQTAIKLTESLMKMGVKILGTKAEDVDAAEDRELFDEILEKTKIPRAAGGTV
                     FTAEEAKEVANRLGYPVLVRPSYVLGGQGMKIAFNDDEIEEFIGIINRIAQDHPILVD
                     KYLQGKEIEVDAVCDGTDILIPGIMEHIERTGVHSGDSISVYPAPTISDHVKETIVEY
                     TKRLAQALHVIGLINIQFIAMNEEVYVIEVNPRSSRTVPYISKVTGIPIVDLATKVII
                     GNTIRGLGYEPGLAPTADYIAIKMPVFSFEKLRGAEISLGPEMKSTGECLGIAKTFNE
                     ALYKAFLGAGVTLPKYKQMIMTVKDADKPEAVGVAKRFEALGYKIYATRSTAKYLQEH
                     GVNALRVNKITQESPNVMDLILGHKIDLVIDTPTQGNGDKTRDGFLIRRNAIETGVYC
                     ITAMDTANALARSLETAMDTLTPVDIAKVKNL"
BASE COUNT          665 a          842 c          671 g          800 t
ORIGIN      
        1 tcgcaatggg tgttgaagca atgtacggag ttgacattca tccatattac aactaatccc
       61 ggagcaacgc catgacaaca gaaaatttta ccaaaaaaga gcctgtaacg gtgcgatgag
      121 ttctcatcca tccgtataca ggctctttct tatttcataa gaaatacctt gacacattaa
      181 tgtacgaaag taggtccttc ctatgaaata agaatcgctc cgcgcggatg cgcagctcgc
      241 ggtatggaaa atatcgcttt gcgaaccgct cgcgcgcgga gaatctcgca agcgagattc
      301 ttttttttgt ttataaattc ttcactttcg caatatcaac cggagtcagc gtatccatcg
      361 ctgtctcaag gcttcttgca agtgcgtttg ccgtatccat cgctgtgata cagtacacgc
      421 cggtctcgat cgcatttcgg cggatcaaaa agccgtcgcg cgtcttgtct ccattgccct
      481 gtgtcggagt atcgatcaca agatcaatct tatgaccaag gatcagatcc atgacattcg
      541 gggattcctg tgtgatctta ttgacacgca gcgcattcac cccatgctcc tgcaaatatt
      601 ttgcggtgct tctggtcgcg tagatcttgt agccgagcgc ttcaaagcgc tttgcaacac
      661 caacggcctc cggcttgtcc gcatccttta ccgtcataat catctgctta tatttcggca
      721 gtgtcactcc ggcgccaagg aacgccttat acaatgcctc gttgaatgtc tttgcaatac
      781 caaggcactc acccgtggac ttcatctccg gtccaaggga aatctccgct ccgcgcagct
      841 tctcaaacga gaataccggc atcttgatgg caatgtaatc agccgtcggt gcaagtcccg
      901 gctcatagcc aagtccgcgg atcgtatttc cgataatgac cttcgttgcg agatcgacaa
      961 tcggaatacc ggtcaccttg ctgatgtacg gcacggttct tgaagatctc gggtttacct
     1021 cgatgacata gacttcttca ttcatcgcaa taaactggat gttgatcagt ccgatgacat
     1081 gcagtgcctg tgccaggcgc ttcgtatatt ccacgatcgt ctcttttaca tggtcgctga
     1141 tcgtcggcgc cggatagacg gaaatactgt ctcccgaatg gacaccggtt ctctcgatgt
     1201 gctccatgat acccggaatc aggatgtccg ttccgtcgca tacggcatcg acctcgatct
     1261 ccttgccctg cagatatttg tcaacaagga tcgggtgatc ctgtgcaatc cggttgatga
     1321 tgccgatgaa ttcctcgatc tcgtcatcgt taaacgcgat cttcatgccc tgtccgccga
     1381 gtacatagga cggacgcaca agcaccggat agcccagacg gttcgccact tccttcgctt
     1441 cctccgcggt aaataccgtt ccgcctgctg ctctcggaat tttggtcttc tcgagaatct
     1501 catcgaagag ctcacgatcc tccgcagcgt cgacgtcctc cgccttggtt ccgaggatct
     1561 tcacgcccat cttcatcagg ctctccgtca gcttgatcgc agtctgtcca ccaaactgca
     1621 ccaccgcacc gtccggcttc tcaagcttta cgatggcttc cacatcctcc ggtgtcaatg
     1681 gctcaaagta cagcttatcc gcaatatcaa agtctgtcga tacggtctcc gggttgttgt
     1741 tgacgatgac ggtctcccag ccttcttttg cgaatgccca ggtacaatgt acggagcagt
     1801 aatcaaactc gattccctgt ccgatacgga tcggtccgga accaagtacc agcaccttct
     1861 tgttcggatg tgtctcttcc gcctcattct cgctgccgaa tacggaatag tagtacggtg
     1921 tggtcgcctc gaactccgcc gcacaggtat ctaccatctt gtatgctgcc acgataccgt
     1981 tagcgtagcg catatcgtgg atctctgtct cggtcttgcc ggtcagttct gcgatcacct
     2041 tatccgggaa ctcaattcgt tttgcctctt ttaacagttc taccgtaagc ggctcgctct
     2101 ttaacttctg ctccatctca accaggattg ccagcttatc gatgaaccac ttatcaatct
     2161 tggtaatatc gtgaatcaca tcgtaatcca taccacggcg cagtgcctct gcgatcaccc
     2221 agattctccg gtcatccaca acgtgaagct gttttaccag ttcctctctg gaaagtccgg
     2281 taaaatcata ggacatcagg ctgtccacat gctgctccaa ggaacggatc gccttcatca
     2341 aggctccctc gaagttattg caaatactca taacctctcc ggttgccttc atctgtgtgg
     2401 tcagtgtacg ttttgcggta atgaatttat cgaacggcag tctcggaatc ttgacaacgc
     2461 agtagtcaag catcggctca aagcttgcgt aggtttttcc ggtgatggcg ttcttgatct
     2521 cgtcaagcgt atagccaagc gcgatctttg cagcaacttt tgcaatcgga taaccggtcg
     2581 ccttggatgc aagcgcggag gaacgggaca ctcgcgggtt tacctcgatg acgcaatact
     2641 caaaactctc cggattgagt gcatactgca cattacatcc gcccgtgata tttaattcag
     2701 atataatatt taaagcagag gtacggagca tctgatattc cttgtcgccc agcgtctgtg
     2761 acggtgcgac tacgatggag tcaccggtgt gcacgccgac cggatcaata ttctccatat
     2821 tacataccgt gatgcagttg ccgttcgcat cacgcattac ctcgtactcg atctctttcc
     2881 agcctgcgat gcagcgctcc acgagcacct ctccgacacg gcttaaacgg agtccgtttg
     2941 tcaggatctc gatcagctcg gtctcatcgt gtgcaata
//
LOCUS       DBMDQJ010000277         2968 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_11941_length_2968_cov_1.396233, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000277 DBMDQJ010000000
VERSION     DBMDQJ010000277.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11941_length_2968_cov_1.396233"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2212
                     /locus_tag="ACZO9R_06505"
     CDS             <1..2212
                     /locus_tag="ACZO9R_06505"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007884841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HIR0912752.1"
                     /translation="LSQISEDEIDLLENYVLAAGIRGKKKWEARFIYTTHEVTEEDLE
                     ALNELRIRVMEPFSELTAVFRKKGATVRECTTALYQFIVSLQIEQQLKYRENQYKEAK
                     DLAKAKEYAQIYRIVMDILDKMATLLGDEVMDISEYAKVLEAGFEAAKVGVIPGGYDC
                     MMIGDIERTRLDDIKVLFIVGVNDGIVPKSDNRGGIISQLEREELAKNHMELAPTARE
                     RVFIQRFYLYLAVTKPSKKLYLTYARVGSDGSAKRPSYFIHMIEGLFPGLKAELITGE
                     QVDETLVTPESSMAFLLEGLYQEDAGWNDEKEVLWQALLHWYEKEPAYREQVKAILDA
                     AELKYHAEPISRAVAKALYGNELKNSVTRLEQYAACACAHFLTYGLRLQERELFEFRM
                     ADMGNIYHDALWHFAERIRRSSYTWYDLPEQMREAWIEESMNDAIAGCANVGAFDEAK
                     NRYLLSRMKDTIRRTVWALTIQVQKGRFTPSDFEVSFSQADHLEAIRFQLTEEEKMRL
                     RGRIDRVDTCETDDKVYVKIIDYKSGNTTFSLLNLYHGLQLQLVVYMNAALELEAKKH
                     PGKRAVPAGMFYYHIEDPMVDGSGTESEEEIRQAVLEELKPNGLVNDDPEIYGAMDTE
                     LSGSSAVIPVALKADGSLKATSKTASTEEFETMSDYVKETVTKAGRRILGGDVAAAPY
                     ELDGRSGCDYCPYHMVCGFDARIPGFSYRKLEKPDGAEAILAKMRETRSEEKRQ"
     gene            2213..>2968
                     /locus_tag="ACZO9R_06510"
     CDS             2213..>2968
                     /locus_tag="ACZO9R_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR0912753.1"
                     /translation="MGVSWTREQQQVIDLRKRNILVSAAAGSGKTAVLVERIIKMITE
                     GEHPVDIDRLLVVTFTNAAAAEMRERIGAAIEKALEEHPDSEHLQRQLTLIHNAQITT
                     IDSFCLYVIRNHFHEIDLEPDFRIGDEGELKLLKEDVLRGVLESNYEQMTPEFAAFAK
                     GYVSGKSDAKVAEMILSLYEFSMSYPWPMEWLTSGICGYEISDVQEMAEAPWMQPLVL
                     HLRSVLADLVKKLRIAHVLTREEDGPNIYEKVIS"
BASE COUNT          788 a          591 c          918 g          671 t
ORIGIN      
        1 attatcacaa atcagtgagg atgagattga cctgctggaa aattatgtac tggcagcagg
       61 catccgtgga aagaaaaaat gggaggcaag atttatttat accacccatg aggttacgga
      121 agaagattta gaagcattaa atgaactccg gatccgtgta atggagccat tttcggaatt
      181 gacggctgta tttcgtaaaa aaggtgctac cgtacgggaa tgcacgacag cactctatca
      241 gtttattgtg tccttacaga tagaacagca gttaaaatac cgggagaatc agtataaaga
      301 ggcgaaggac ttagccaagg caaaggaata tgcgcagatt tatcgcatcg taatggatat
      361 tttggataag atggcaacgc ttctgggtga tgaagtcatg gacatatccg aatatgccaa
      421 ggtcttagag gctggctttg aagcagccaa agtaggcgtg attccgggtg gctatgactg
      481 catgatgatt ggtgatatcg aaagaacccg tcttgacgat ataaaagtat tgtttatcgt
      541 gggagtcaat gatggtattg tgccaaaatc cgataatcgt ggcggcatca tttcccagct
      601 ggaaagagaa gaacttgcga aaaatcatat ggaactggct cctacagcaa gggaacgtgt
      661 ttttatccag cgtttttacc tttatcttgc agtgacaaag ccatcgaaaa aactttatct
      721 tacctatgca agggtaggca gtgatggaag tgccaagcgc ccgtcctatt ttattcatat
      781 gattgaggga ctgttcccgg ggttaaaagc agagctgata acgggagaac aggttgatga
      841 aacactggta acaccggaga gcagcatggc atttctgtta gagggactgt atcaggaaga
      901 tgccgggtgg aatgatgaga aggaagtact ctggcaggct ttgctgcact ggtatgaaaa
      961 ggaaccggca tatcgggagc aggtaaaagc tattcttgat gcagcagagc ttaaatatca
     1021 tgcagagcca atcagccggg ctgtggcaaa ggctctttat ggaaatgagt taaagaacag
     1081 tgttacgcgg ttggaacagt atgcagcctg tgcctgtgcc catttcctga cttatggact
     1141 gcgtttacag gaacgggaac tctttgaatt ccgtatggcg gatatgggaa atatctatca
     1201 tgacgcgctg tggcattttg cggagcgcat ccgcaggtct tcgtatacct ggtatgatct
     1261 gcccgaacag atgcgcgagg cgtggattga ggagagcatg aatgacgcga ttgcgggctg
     1321 cgcgaatgtg ggcgcattcg acgaggcgaa gaaccgctat ctgctgtcgc gcatgaaaga
     1381 cacgatccgg cgcacggtgt gggcgctcac gatccaggtg cagaaggggc gttttacgcc
     1441 gagtgatttt gaggtgtcgt tttcgcaggc cgatcatctg gaggcaatcc gttttcagct
     1501 gactgaggag gaaaagatgc gtctgcgggg caggattgac cgtgtggata cctgtgagac
     1561 ggacgataag gtgtatgtaa agatcattga ttacaaatcg ggcaacacca cattttccct
     1621 gctgaatctg tatcacgggc tgcagctgca gctggtggtt tatatgaatg cggcgctcga
     1681 actggaggca aaaaagcatc cgggaaaacg ggcggtgcct gcgggaatgt tttactatca
     1741 cattgaggat cccatggtcg acggcagcgg tacggagtcg gaggaggaga tccggcaggc
     1801 cgtgctggag gaattaaagc cgaacgggct ggtcaatgac gatcccgaga tatacggggc
     1861 gatggatacg gaactttccg gaagctccgc agtcattcct gtggcgctta aagcggacgg
     1921 ttccttaaaa gcgacgtcca agacggcgag tacggaagaa tttgagacaa tgtcggacta
     1981 cgtgaaggag accgtgacga aggcgggcag aagaatccta ggcggagatg tggcggcggc
     2041 accgtatgaa ctcgacggga gaagcggctg cgattattgt ccgtatcaca tggtgtgcgg
     2101 atttgacgcg cggataccgg gcttttctta ccggaagtta gaaaagccgg acggtgccga
     2161 ggcgattctt gcgaaaatga gagagacgcg aagcgaggag aagagacagt agatgggtgt
     2221 ttcatggacc agagaacaac aacaggtaat cgatttaaga aagcgcaaca ttctggtatc
     2281 ggcagctgcc ggttcgggta aaacggcggt tttagtcgag cgcattatca agatgatcac
     2341 ggagggagaa catccggtgg acatcgaccg gcttttggtg gtgacgttta cgaatgcggc
     2401 tgcggcggaa atgcgggagc gcatcggggc ggcgatcgag aaagcgctgg aggagcatcc
     2461 ggattcggag catctgcaac gccagctgac gttaatccac aatgcgcaga tcacgactat
     2521 cgacagtttt tgtctgtatg tgatccgcaa tcatttccac gagattgacc tggagccgga
     2581 ttttaggatc ggggacgagg gagagttaaa gctgttaaaa gaggacgtgc tgcgcggcgt
     2641 gctggagagc aattacgaac agatgacacc ggagtttgcg gcatttgcaa aagggtatgt
     2701 gtcggggaag agtgatgcga aggtggcgga gatgatcctc tcgctctatg agttctccat
     2761 gagctacccg tggccgatgg agtggcttac ttccggcatc tgtggctatg agatatcgga
     2821 tgtgcaggag atggcagaag cgccatggat gcagccgctc gtgctgcatc tgcggtcggt
     2881 gctggcggat ctggtaaaaa agcttcgcat cgcgcatgtg cttacgcggg aggaagacgg
     2941 accgaatatc tatgagaagg tgatctcg
//
LOCUS       DBMDQJ010000278         2955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12011_length_2955_cov_1.542046, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000278 DBMDQJ010000000
VERSION     DBMDQJ010000278.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12011_length_2955_cov_1.542046"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2108)
                     /locus_tag="ACZO9R_06515"
     CDS             complement(<1..2108)
                     /locus_tag="ACZO9R_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR0912754.1"
                     /translation="MGKIEFLDQQGSFRVYQPEDYSGLYFPLAGDGGLKSCITPTLGG
                     DSKRDQNHFLMEPVSIENLHNNKNTRNFWCRIKNRGYWSACGSSAEAEFLRFTGEQEK
                     SVLEAGFMWQKLTRTSAVHGLKAEITSFVTVDGAMEVMMTELTNITEEPLEMTPIAVM
                     PVYGRSADNIRDHRHVTSLLHRICTTDYGVEVTPAMSFDERGHQKNHTTYFVYGSTGE
                     GEAPAAFYPTVGEFIGEGGSYLIPEAVRTEKPGAVAGSKAEGKEAVGALKFAEILLKP
                     HETVCYLTLGGMTDERSQIEPLTASLRQRSQVKEAYDEVKRYWTKKVNITFKTGNPDA
                     DNYLKWITFQPILRRIYGCSFLPYHDYGKGGRGWRDLWQDCLALLLMDPSAVRQMIID
                     NYGGVRVDGTNATIIGNAQGEFIADRNHITRVWMDHAFWPFVTTKFYIDQTGDLEILF
                     EKVPYFKDLQSKRGTDHDTGWDETYGKCQRTEGGVVYFGSVLEHLLLQNLCAFYDVGA
                     HNEMRLHGADWNDALDMAWENGESVAFTSAYAGNLKEIAHCIRLLEQETGCKRFEIAE
                     EMGMLFAGGRELYENVEKKRGILDAYLEKCAHNLSGQTMIVKAEQICSNLEEKADWLM
                     EHIRVQEWIAEDEAHGWFNGYYDDHKNPVEGCKDGKVRMMLTSQVFAIMSGTATPDQI
                     RKICQSVDKYLYDEKAGGYRL"
     gene            complement(2126..>2955)
                     /locus_tag="ACZO9R_06520"
     CDS             complement(2126..>2955)
                     /locus_tag="ACZO9R_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR0912755.1"
                     /translation="ETASYLDEKQEELGKLLQELCWNEDRFIRGFTEAGEVIGKRTDP
                     EANMWLNPQSWAVISGLAKADQADMALERVYEKLNTPYGAILMDPPYHAHAFDGALAV
                     IYNPGAKENAGIFSQSQGWIILAEALRGHGDRAFTYFMENAPAAQNDRAQIRVIEPYC
                     YGQFTEGKASPNFGRSHVHWLTGTASTVMVGCVEGILGMRPDFYGLHLTPAIPHEWEE
                     FEVNKVFRGCQLHIVVKNPGHVQSGCDRLLVNGVRMQGDYIPQEVLKKQTEIVLYMS"
BASE COUNT          675 a          890 c          559 g          831 t
ORIGIN      
        1 agacggtagc cgcccgcctt ctcatcatac agatatttat cgacactctg gcagattttg
       61 cggatctgat ccggtgttgc cgtgccgctc ataatcgcaa agacctgact cgtcagcatc
      121 atccgtacct ttccgtcttt acagccctcc accggattct tatgatcatc ataatagcca
      181 ttaaaccatc cgtgtgcctc atcctcagca atccactcct gcacccttat atgctccatc
      241 agccagtctg ctttttcctc cagattgctg caaatctgct ctgcttttac gatcatggtc
      301 tgaccactga gattatgtgc acatttctcc agataggcgt ccagtatccc tcgtttcttt
      361 tccacattct catacagctc cctgccacct gcaaacagca ttcccatctc ttctgcaatc
      421 tcaaaacgct tacagccggt ctcctgctct aacagccgga tacagtgcgc aatctctttt
      481 aaatttcccg cataagcact tgtaaatgcc acgctttctc cattttccca tgccatatcc
      541 agcgcgtcat tccagtctgc cccgtgcagc cgcatctcat tgtgcgcacc gacatcgtag
      601 aatgcacaga gattctgaag aagcagatgt tccagtacac ttccgaaata gacgacgcca
      661 ccctctgtgc gctggcattt tccatatgtc tcgtcccatc cggtatcgtg atctgtgcct
      721 cttttggact gcagatcctt aaaatacgga accttctcaa aaagaatctc gagatctcct
      781 gtctggtcaa tataaaattt tgtcgtcaca aacggccaga acgcatgatc catccacaca
      841 cgggtaatat gattccggtc cgcaataaac tccccctgcg catttccgat aattgtggca
      901 ttggttccgt ccacccggac accgccgtaa ttgtcgatga tcatctgccg gactgcggaa
      961 gggtccatga gaagcaatgc cagacaatcc tgccacagat cccgccagcc acgtcctcct
     1021 tttccgtaat catgatacgg caggaacgaa cagccgtaaa ttctccgcag aattggctgg
     1081 aacgtaatcc acttaagata attgtctgca tccggattac ccgttttaaa tgtgatgttt
     1141 actttctttg tccagtaccg ttttacttca tcataagctt cctttacctg gcttctttgt
     1201 ctgagagatg ctgtaagagg ttcgatctgt gatctctcgt cggtcattcc gcccaaagtc
     1261 agatagcaga ctgtctcatg tggttttaat aaaatctccg caaacttaag agctcccact
     1321 gcttcttttc cctctgcctt acttcctgcc acagctcccg gcttttctgt ccggactgcc
     1381 tccggtatga gataggaacc tccctccccg ataaactccc cgacggtcgg gtaaaaagct
     1441 gccggtgcct ctccctctcc ggtggaacca tatacaaaat acgttgtatg attcttctga
     1501 tgcccccgct catcaaacga catcgcaggc gtcacctcca ccccataatc cgtggtacag
     1561 atccggtgca acagggaagt cacatggcgg tgatccctga tattgtccgc acttcttcca
     1621 tagaccggca tcactgcaat cggtgtcatt tcaagcggtt cctctgttat attggtaagt
     1681 tccgtcatca tcacttccat cgctccgtct accgtcacaa aagaagtaat ctctgctttt
     1741 agtccatgga ctgcggatgt ccgcgtcagt ttctgccaca taaatccggc ttccagtacg
     1801 cttttctcct gttctccggt aaaccgcaga aattctgcct ctgccgacga accgcaggct
     1861 gaccagtatc cccggttttt gatacggcac cagaaatttc ttgtattttt attgttatgg
     1921 agattttcaa tactgaccgg ctccatcagg aaatggttct gatccctttt gctgtcgccg
     1981 cccagtgtcg gtgtaatgca ggattttaac ccaccgtctc ctgcaagagg aaaatacaat
     2041 ccactgtagt cttccggctg atacacccgg aagcttccct gctgatccaa aaattctatc
     2101 tttcccacga atctttcctc ccatcttatg acatatacaa tacgatttct gtctgctttt
     2161 tcaatacttc ctgcggaata taatctccct gcatcctgac tccatttaca agtagtctgt
     2221 cgcatcccga ctgcacatgt cccggatttt tcacaacaat atgtaactgg cagcctctga
     2281 aaactttatt tacttcaaac tcctcccatt catgcggaat tgcaggcgta agatgcagtc
     2341 catagaaatc cgggcgcata cccagaattc cctccacaca gccgaccata accgtggaag
     2401 cagttcccgt gagccagtgc acatgtgacc ttccgaaatt cggacttgcc tttccctccg
     2461 tgaactgtcc atagcaataa ggttcaatca cgcggatctg tgcacggtca ttctgtgcag
     2521 ccggtgcatt ttccataaaa taagtaaatg ctctgtctcc atgtccccgc aatgcctccg
     2581 ccaggataat ccatccctgc gactgtgaaa aaatacctgc attttctttt gcacccggat
     2641 tatagatgac agccagtgct ccgtcaaagg cgtgcgcatg atacggcggg tccatcaata
     2701 ttgcaccata cggcgtgttt aatttctcat ataccctctc aagcgccata tccgcctgat
     2761 ccgcttttgc cagcccgctg atcaccgccc agctctgcgg attcagccac atatttgcct
     2821 ccggatctgt gcgctttccg atcacttcgc ctgcttctgt aaatccacgg ataaagcgat
     2881 cctcattcca gcagagttcc tgcagcaatt tccccagttc ctcctgcttt tcatcaagat
     2941 aggaagccgt ttctt
//
LOCUS       DBMDQJ010000279         2955 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12015_length_2955_cov_1.237561, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000279 DBMDQJ010000000
VERSION     DBMDQJ010000279.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12015_length_2955_cov_1.237561"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="ACZO9R_06525"
     CDS             <1..404
                     /locus_tag="ACZO9R_06525"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase family protein"
                     /protein_id="HIR0912756.1"
                     /translation="AEKNLEKVQIADEVTDVICEECGRNMVVKYGPHGKFLACPGFPE
                     CRNTKPYLEKIGVPCPKCGKDVVLRKTKKGRKYFGCENNPECDFMSWSRPVAEKCPKC
                     GGYMVVKGNKIACADAGCGYTRDRKADEDTK"
     gene            512..1294
                     /gene="codY"
                     /locus_tag="ACZO9R_06530"
     CDS             512..1294
                     /gene="codY"
                     /locus_tag="ACZO9R_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HIR0912757.1"
                     /translation="MSVQLLDKTRKINKLLHNNSSSKVVFNDICDVLKDILASNILVI
                     SKKGKVLGTGVCPGVDEIQELIVDEVGGFIDPLLNERLLGILSTKENVNLETLGFVDH
                     VKEYTAIITPIDIAGERLGTLFVYREDRQYDIDDIILSEYGTTVVGLEMMRSVNEENA
                     EETRKVQIVKSAISTLSFSELEAIIHIFDEMDGKEGILVASKIADRVGITRSVIVNAL
                     RKFESAGVIESRSSGMKGTYIKVLNDVVFDELDKIKKQNQKK"
     gene            1517..>1689
                     /locus_tag="ACZO9R_06535"
     CDS             1517..>1689
                     /locus_tag="ACZO9R_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HIR0912758.1"
                     /translation="MITTNAFDYINIMDKAADASWKRESAISNNIANTDTPGYKRQDV
                     DFESALKRELGSS"
     assembly_gap    1690..1699
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1700..1906
                     /locus_tag="ACZO9R_06540"
     CDS             <1700..1906
                     /locus_tag="ACZO9R_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HIR0912759.1"
                     /translation="KKVRGLNSDLSGLDVSTYTDSSNYSYRLDRNNVDVDTEQVELAS
                     EQLRYEMLTTSINEEFNRMKLVLK"
     gene            1921..2361
                     /gene="flgC"
                     /locus_tag="ACZO9R_06545"
     CDS             1921..2361
                     /gene="flgC"
                     /locus_tag="ACZO9R_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HIR0912760.1"
                     /translation="MGLFQSFDISASGMTAERFRIDTIAENIANVNTTRTEDGEPYRR
                     KIVTFAEKDLTPFSKYYQSSRARAVGNGVKVTSVKEDTETDFTMEYDPSHPDADENGY
                     VRYPNVNTVTEMTNLIDASRAYEANTTAFNASKSMIQAALKIGQ"
     gene            2395..2709
                     /gene="fliE"
                     /locus_tag="ACZO9R_06550"
     CDS             2395..2709
                     /gene="fliE"
                     /locus_tag="ACZO9R_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HIR0912761.1"
                     /translation="MDISLLTNATSPDYMSAVTQKTLLSVDTDESFDSVLSSAINMLN
                     ETNDLQNDSQSAKIQFALGEADNPHDMQIAAAKAYEALQYTVAVRDKMLDAYKEIMNM
                     QI"
     gene            2713..>2955
                     /locus_tag="ACZO9R_06555"
     CDS             2713..>2955
                     /locus_tag="ACZO9R_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HIR0912762.1"
                     /translation="MELEIMPEQVQKILTKITDWWKKFTTKQRILLGSIVAVIILALV
                     ILAVVVGKPNMVTLIECSSTAQAGEVKDLLDNDGSIS"
BASE COUNT          887 a          562 c          816 g          680 t
ORIGIN      
        1 tcgcggagaa aaatctggaa aaagtgcaga tcgcggatga agtgaccgat gtgatctgtg
       61 aggagtgcgg ccgcaatatg gtggtaaaat acggaccgca cggaaaattc ctggcatgcc
      121 cgggcttccc ggagtgcagg aacacgaagc cgtatctgga aaaaatcggc gtgccatgtc
      181 cgaagtgcgg aaaagacgtt gtcctccgca agacgaagaa aggcagaaag tatttcggct
      241 gcgagaataa tccggagtgc gattttatga gctggtcacg cccggtagcg gagaagtgcc
      301 cgaagtgcgg cggctatatg gtggtaaaag gcaataagat cgcctgtgca gatgcgggat
      361 gcggctacac ccgtgacaga aaagcggacg aggatacaaa atagttaaaa aaatatgtca
      421 aatattacga aaatattgaa ttttcagaat tgacgtgtta aaatatgaaa tataacgaaa
      481 aagagattcg aaacgaaatg ggggcacgga catgagtgtt caattgttgg acaaaaccag
      541 aaaaatcaat aagctgttgc acaataacag ctcttcaaag gtcgtgttta atgatatctg
      601 cgatgtgttg aaggatattc ttgcatccaa tattctggtc atcagcaaaa agggaaaggt
      661 tcttggaacg ggcgtgtgtc ccggagtgga tgagatccag gaattgatcg tggacgaggt
      721 gggaggtttc atcgacccgc tgctaaacga acgtctgctc ggaatcctct ctaccaagga
      781 gaatgtcaat cttgagacgt taggtttcgt agatcacgtg aaggaataca ctgcgatcat
      841 cacgccgatt gatattgcgg gtgagcggct tggcacgctg tttgtctaca gagaagacag
      901 acagtacgat attgacgata tcattttgag cgaatacggg acaaccgtgg tcggtctgga
      961 aatgatgcgc tccgtgaatg aggagaatgc ggaggagaca agaaaggttc agattgtcaa
     1021 atctgcaatc agcacgttgt cattttcgga attggaagcg atcattcata tttttgatga
     1081 gatggacggc aaggagggaa tccttgtggc aagcaagatt gccgaccgcg tgggtatcac
     1141 acgatccgtc attgtgaatg cactgcgcaa gttcgagagt gcaggcgtga ttgagtcgcg
     1201 ctcttccggc atgaagggaa cctacatcaa agtattgaac gacgttgtat tcgacgaact
     1261 cgacaagatc aaaaagcaga atcagaaaaa ataaacagga cgaaagggcg tatcgaaggg
     1321 tgcgctcttt tttatttccg tggaacgtgg ctttttatgc gggtttattt gacaattttg
     1381 ctaatatctt gtacttatta tgcaaaaatc tacaagatag tgtaaaaaat aacaaaaatc
     1441 tcttgcaatt acgaaaccat attttataat ggaataagat agtttggaaa actaggtgaa
     1501 gcggagggcg atacatatga taactacgaa tgcgttcgac tatattaaca tcatggataa
     1561 ggcggcggat gccagctgga aacgtgagtc ggcaatttcc aacaacatag caaatacgga
     1621 tactccgggc tataagagac aggatgtgga ttttgaatct gcgctgaaac gggagcttgg
     1681 cagctcgaan nnnnnnnnna agaaagtgcg gggactgaac tcggatcttt cgggcttaga
     1741 tgtgtctacc tatacggatt cctccaatta ttcctatcgt ctggaccgca ataatgtgga
     1801 tgtggatacg gagcaggtgg aactggcatc ggagcagctc cgctatgaga tgctgacgac
     1861 atcgatcaac gaagaattca atcgtatgaa attagtgctt aaataaacag gaggtagcat
     1921 atgggattat ttcagtcttt tgacatcagt gcgagcggta tgacggcaga gcgcttccgc
     1981 atcgacacca tcgcggagaa tattgccaat gtcaatacaa cgcgtacgga ggacggtgaa
     2041 ccgtaccgcc gcaagattgt gacgtttgcg gaaaaggatc tgactccgtt cagcaaatat
     2101 tatcagtcat ccagagcgcg ggctgtaggc aacggcgtga aggttacctc cgtcaaggaa
     2161 gatacagaga ccgattttac aatggagtat gatccgtccc atccggatgc ggatgagaac
     2221 ggctatgtgc gttatccgaa tgtgaatacg gtgacagaga tgacgaacct gattgacgcg
     2281 tcccgtgcat atgaggcgaa cacgacggcg tttaatgcca gcaagagcat gattcaggca
     2341 gcacttaaga tcggtcagta agacatagat taggatgaaa cgggggaaat acagatggat
     2401 atcagtttat tgacgaatgc cacttcacca gattacatga gtgcggtaac acagaagaca
     2461 ctgttgtctg tggacaccga tgagagcttt gactcggtgt tgtcttcggc aatcaatatg
     2521 ctcaacgaga cgaatgatct gcagaatgat tcgcagtcgg caaagatcca gttcgcgctg
     2581 ggcgaggcgg acaatcctca cgatatgcag attgcggcag ccaaggcgta tgaggcgctg
     2641 cagtacacgg ttgcagtgag agataagatg cttgatgcgt acaaagagat catgaacatg
     2701 cagatttaat gaatggagtt agaaattatg ccggaacagg tgcaaaagat tttaacgaag
     2761 ataacggatt ggtggaaaaa gtttacaacc aagcagagaa tcctgctcgg aagcattgtg
     2821 gcggtaatta tactggcatt ggtgattctg gcggtggttg tcgggaagcc gaacatggta
     2881 acgcttatcg agtgctcctc cacagcgcag gcgggtgaag taaaagacct tctggacaat
     2941 gacggaagca tctcc
//
LOCUS       DBMDQJ010000280         2940 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12091_length_2940_cov_1.068334, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000280 DBMDQJ010000000
VERSION     DBMDQJ010000280.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12091_length_2940_cov_1.068334"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="ACZO9R_06560"
     CDS             <1..354
                     /locus_tag="ACZO9R_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein"
                     /protein_id="HIR0912763.1"
                     /translation="GFPEANISIIAYDEPIFQYSSGGRTLSDYLQIILAVLILLLLGY
                     VVFRSTRKEKQAEMEEELSVESLLESTKENQEPLEDIGYNEKSETRLLIEKFVEEKPE
                     AAASLLRNWLNEEWE"
     gene            356..1366
                     /gene="fliG"
                     /locus_tag="ACZO9R_06565"
     CDS             356..1366
                     /gene="fliG"
                     /locus_tag="ACZO9R_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HIR0912764.1"
                     /translation="MAKNEEFTGLQKAAILLITLGPERSADIFKHLKEDEIEELTLEI
                     ANTRSISPQVKEDVLNEFYQICLAQQYIAEGGIGYAKELLDKALGADKAQEVISKLTA
                     SLQVRPFEFVRKTDPSQLLNFIQDEHPQTIAMILSYLTAPQASMVLGALPPEKQADVA
                     KRIAMMDRTSPDVIKEVERVLERKLSSLVNQDYTIVGGVDAIVSILNAVDRSTEKHIM
                     ESLEIEEPELADEIRKKMFVFEDILLLDDRAIQRVLRDVDNADLGVALKAANEEVQNV
                     IFKNLSKRLAAMIKEDMEFMGPVRMKDVEEAQQKIVSVIRKLEDAGEIVISRGGGDEI
                     VV"
     gene            1359..2168
                     /locus_tag="ACZO9R_06570"
     CDS             1359..2168
                     /locus_tag="ACZO9R_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliH/SctL family protein"
                     /protein_id="HIR0912765.1"
                     /translation="MSNLYKQWFVQTDSTNTRIIDSNVILQQHLKQDLPQTQTPAQNV
                     QEEEGGFSAGLVTGDPGQVIKAEPQIDYEAKAKAKAEEILAAAQQNADAILAQANEDA
                     EEIRTSAKTQGYEEGRASAEQENEQLRVQLEESYRKKQGDLDSDYRDKREHMEQDLVD
                     VIVTVFEKVFHIQFDDKKEILMHLIEDAILNIEDDKRFRVKVAEDNALFLENHKTEIL
                     DRVGHDIELEIVSDATVEGNDCTIETDSGVFNCSLGTQLENLIKDIRSLCS"
     gene            2181..>2940
                     /gene="fliI"
                     /locus_tag="ACZO9R_06575"
     CDS             2181..>2940
                     /gene="fliI"
                     /locus_tag="ACZO9R_06575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079545.1"
                     /note="involved in type III protein export during
                     flagellum assembly; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HIR0912766.1"
                     /translation="MTHQFEKYLQLGEKTYFNRLGKVVKIVGLTIESVGPDAKLNDLC
                     RIVIDREKDHAVMAEVVGFRDKRLLLMPFENVEGIGVGCIVENTGHPLGVLVGDGVLG
                     HTLDGIGRPTDGGVIEGGCEYPVEADPPDPMSRKIISEVLPLGVKAVDGLITVGKGQR
                     IGIFAGSGVGKSTLLGMFARNTKADINVIALIGERGREVREFIERDLGEEGMKRSVVV
                     VATSDKPALIRNKAAKTATAIAEYFRDQGKDVLLM"
BASE COUNT          804 a          618 c          881 g          637 t
ORIGIN      
        1 gggtttcctg aggcaaacat cagcatcatc gcttatgacg agccgatttt ccagtattct
       61 tccggtggaa gaaccctgtc ggattatctg cagatcatac ttgcggtact gattcttctc
      121 ctgcttggtt atgtggtatt ccgcagcacc agaaaggaga aacaggcaga gatggaggag
      181 gaactttccg tggaaagcct gctggaatcc acaaaagaga accaggagcc gcttgaggac
      241 atcggctaca atgagaagtc cgagacgcgt ttactgatcg aaaaattcgt agaagaaaaa
      301 ccggaggcgg ctgcatcgtt actgcgcaac tggctcaacg aggagtggga gtaatatggc
      361 aaaaaacgag gaatttacag gactgcaaaa agctgcaatt cttctgatta cactgggacc
      421 ggagcggtca gcagatatct ttaagcatct taaggaggac gagatcgagg aacttaccct
      481 tgagatcgca aataccagaa gtatttcacc gcaggtcaag gaggatgtct taaacgagtt
      541 ctatcagatc tgtctggctc agcagtatat tgcggagggc ggtatcggtt atgcgaagga
      601 actgctcgac aaggcgcttg gcgcggacaa ggcacaggag gttatctcca agctgacagc
      661 atcccttcag gtgcgtccgt tcgagtttgt gcgcaagaca gatccaagcc agctgctgaa
      721 cttcattcag gacgaacatc cgcagaccat tgccatgatt ctgtcctatc tgacagcacc
      781 gcaggcgtcg atggttctgg gggcgctgcc gccggaaaaa caggcggatg tcgcaaaacg
      841 tatcgcgatg atggatcgta catcgccgga tgttatcaag gaagtggagc gggtacttga
      901 gagaaagctt tcttccctgg tcaaccagga ttacacaatc gtcggcggcg tggacgctat
      961 tgtcagcatc ttaaatgcag tcgaccgttc gaccgagaaa catatcatgg agtctctcga
     1021 gatcgaagag ccggagcttg cagacgagat ccgcaagaag atgttcgtat tcgaggatat
     1081 tctgctgctt gatgaccgtg caatccagcg tgtactgcga gatgtggata atgcagacct
     1141 tggcgttgcc ttgaaggcgg cgaacgagga agtacagaat gttatattta agaacctctc
     1201 caaacgtctt gctgcgatga tcaaggaaga tatggagttt atgggacctg tgcgaatgaa
     1261 ggatgttgag gaggctcagc agaagatcgt aagcgtcatt cgtaaactcg aggatgcagg
     1321 cgagattgta atctcaagag gcggaggtga cgagatcgtt gtctaatctt tataaacagt
     1381 ggtttgttca gacggattcc acaaacacgc gcatcatcga ttccaatgtg atcctgcagc
     1441 agcacctgaa acaggatctg ccgcagacac agacaccggc acagaatgtg caggaagagg
     1501 aaggcggatt ttcagccgga cttgtgacgg gtgatccggg tcaggtcatc aaagcggagc
     1561 cgcagattga ttatgaggca aaggcaaagg caaaagccga ggagatcctt gcggcggcac
     1621 agcagaatgc cgatgcgata ctggcacagg caaacgaaga tgccgaggag atccgtacat
     1681 ctgcaaagac gcaggggtac gaggagggca gagcttcggc ggagcaggag aatgagcagc
     1741 ttcgcgtgca gcttgaggag tcctacagga aaaagcaggg agatcttgac agcgattaca
     1801 gggacaaacg cgaacatatg gaacaggatc tggtggatgt catcgttacc gtctttgaaa
     1861 aagtgttcca tattcagttt gacgacaaaa aagagatact gatgcatctg attgaagatg
     1921 ccattttaaa tattgaagac gacaagcggt tccgggtgaa ggtggcggag gacaatgcac
     1981 tgtttctgga gaatcacaag accgagatcc tggatcgtgt gggacatgac attgagcttg
     2041 agattgtctc ggacgcgacg gtggagggaa atgactgtac gatagagacc gattccggcg
     2101 tgtttaactg cagtctcggt acacagcttg agaatctgat caaagatatc cggtctttat
     2161 gttcgtaagg tgagagaatg atgacacatc aatttgaaaa atatttacaa cttggagaaa
     2221 agacctactt caaccgcctt ggaaaggttg taaagattgt cggacttacg attgaatctg
     2281 tcggaccgga tgcaaagtta aacgatctct gcagaattgt cattgacaga gagaaagacc
     2341 acgcggtgat ggcggaggta gtcggattca gggataagag acttttgctg atgccgtttg
     2401 aaaatgtgga aggcatcggg gtaggatgta tcgttgagaa taccggacat ccgcttgggg
     2461 tgcttgtcgg ggacggcgtt ctgggacaca cactggacgg tatcggaaga ccgaccgacg
     2521 gcggagtcat tgagggcgga tgcgaatatc cggtcgaggc ggatccgccg gatccgatga
     2581 gccgtaagat tatctcggag gtgcttccgc ttggtgtaaa ggcggtagac gggctgatta
     2641 ccgtgggaaa aggacagcgt atcggaatct ttgcgggttc cggtgtcgga aagagcaccc
     2701 tgctcggtat gttcgcccgc aatacaaaag cggacatcaa tgtcattgcc ctgatcggag
     2761 agcgtggccg tgaggtgcgc gagtttatcg agagggatct cggggaagag ggaatgaagc
     2821 gttccgtcgt cgtggtggcg acttccgata agccggcact gatccgtaac aaggctgcga
     2881 agacggcgac cgcaatcgcc gaatatttca gggatcaggg aaaagatgta ctgctgatga
//
LOCUS       DBMDQJ010000281         2935 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12109_length_2935_cov_1.616090, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000281 DBMDQJ010000000
VERSION     DBMDQJ010000281.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12109_length_2935_cov_1.616090"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(26..526)
                     /locus_tag="ACZO9R_06580"
     CDS             complement(26..526)
                     /locus_tag="ACZO9R_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912767.1"
                     /translation="MWDKARRILYRGGEILELLMAAAVVAGILVAMITLVPELARYWE
                     HRTIAGAFMEYLDAVFNVVIGIEFLKMLCKPSSANIIEVLIFLIARHMIVQTTTPLED
                     LLSVVSIGILFFFRRFMLMTKPDKDQNVPKLFGVIKSSQSSGTAQAAHVDTEKNAEGS
                     KGAGEK"
     gene            complement(596..860)
                     /gene="ffs"
                     /locus_tag="ACZO9R_06585"
     ncRNA           complement(596..860)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACZO9R_06585"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(903..1382)
                     /gene="tadA"
                     /locus_tag="ACZO9R_06590"
     CDS             complement(903..1382)
                     /gene="tadA"
                     /locus_tag="ACZO9R_06590"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HIR0912768.1"
                     /translation="MTQEEKYMKAAIREAKKAYALEEVPIGCVIVQNDRIIARGYNRR
                     NTEGNTLAHAELTAIKKASKKTGDWRLEDCTMYVTLEPCQMCAGAIVQSRMKKVVIGS
                     MNPKAGCAGSVLNLLQMAQFNHQVEIERGVLEEECSTMLSGFFRELRDKKKRLRENV"
     gene            1480..2073
                     /locus_tag="ACZO9R_06595"
     CDS             1480..2073
                     /locus_tag="ACZO9R_06595"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0912769.1"
                     /translation="MNCNNHITGSLPVHTMYTTDRLILKILTPDDTNEVLAFQCRNRE
                     LFERYEPTRPPYFLTPAHQQAILKCEYDLARKCASIRFYVFLKTNPRIIIGTVCLHDI
                     LRMPYCCSEIGYKFDAAFQHQGYAREAVAKVLNIAFTDLSLHRVFARVMPENTPSLRL
                     LEVIGFTCEGTEYACTQIQGKWEDHIRYALLNPYSSI"
     gene            complement(2058..>2935)
                     /locus_tag="ACZO9R_06600"
     CDS             complement(2058..>2935)
                     /locus_tag="ACZO9R_06600"
                     /inference="COORDINATES: protein motif:HMM:NF040001.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6128 domain-containing protein"
                     /protein_id="HIR0912770.1"
                     /translation="LVGEMKLANGSGDGAVVLKGGRVGASPYGITEMEGLFFVIGEGR
                     ILMSRWREGAPFKVGLSGFREWQPEKLKEKTAEKGAAAETAAQKEVREAAAAQAEQNS
                     QTAAQEMAQGKQGGAAPVQSEQNSRMAAKGMAQDVPSGSRAETAAAGEEMEAIHATEL
                     PMRNLFPEYDWNAVWEELCREHKPAALFEEWDTQCIQLELKDLRSLPKKYWYLGNNSF
                     LLHGFFNYRYLVLGRTAEERWFLGVPGVYQRQERVMAAIFGFPEFFAAGPGGERREGR
                     EPVNHFGCWVRYIEE"
BASE COUNT          630 a          950 c          611 g          744 t
ORIGIN      
        1 cgtcatgcat ctgtcaatca atgtactatt tttccccggc gcctttgcta ccttctgcat
       61 ttttctctgt atccacatgc gccgcctgag ctgtcccgct gctctgtgac gattttatca
      121 cgccaaaaag cttcggtaca ttctgatcct tatccggttt ggtcatcagc ataaaacgcc
      181 ggaagaagaa caggataccg atgctgacca ccgagagcag atcctcaagt ggtgtcgtcg
      241 tctgcacgat catatgacgg gcaatcaaaa agatcagcac ctcaataata ttcgcggaac
      301 ttggtttgca tagcatcttc aagaactcaa tgccgatgac aacattaaac acggcatcca
      361 gatactccat aaacgcaccg gctatcgtcc ggtgttccca gtagcgtgcc agttccggca
      421 caagcgtgat cattgccacc agaatccctg ccacgacggc tgccgccatc agaagctcta
      481 agatctctcc gccacggtac aatatccttc tcgccttatc ccacatggta tctctccctc
      541 ttcccaaaag ttatctgtca gaatctttat aaaaaacact tataaaacgc aaaatccgag
      601 cgcctcactt cgaatccaaa accataaacg ttaccaccac agttagctca gcccaggcac
      661 cctcacggca cacaagagct tctgcttagg gctgctccat tcctgacctg acccggttca
      721 cagattcctg ttgcgtaaga cccagacgtc aacgccactt atgatgggca gctttacagc
      781 ttacctgatc ctctgtttgg catcacccca actatagcgg attgttggta cagggtaccg
      841 ctaccacccc ggctgctcgg agacttaagt atatcctatc tgacgcccga ttgtcaagca
      901 ttctacacgt tttcacgcag tctctttttc ttatcgcgca gttcccggaa gaagccggag
      961 agcatcgtcg aacattcctc ctccagcacg cccctctcga tctcgacctg atgattaaac
     1021 tgtgccatct gaagcaggtt cagcaccgac ccggcgcacc ccgccttcgg gttcatgctg
     1081 ccgatcacga cctttttcat gcggctctgc acaatggccc ccgcacacat ctggcacggc
     1141 tctagcgtga catacatcgt gcagtcttcc agccgccagt cgccggtctt tttgctcgcc
     1201 tttttgatcg cagtaagctc cgcgtgcgcc agcgtatttc cctctgtatt tcttctgttg
     1261 taaccccgcg caatgatcct gtcattctgg acgatcacgc agccgatcgg cacctcctcg
     1321 agcgcgtatg ccttttttgc ctcgcgtatc gccgctttca tatatttttc ttcctgtgtc
     1381 atggacagtt tctccagaaa tgctatacta tagtatagac ttattttagc cgatttgatg
     1441 cattttgtcg atggaacatt ttcggcagag ggagtatgta tgaactgcaa caaccatatt
     1501 accggcagtc ttcctgtcca taccatgtat acaaccgatc gcctgatctt aaaaatactg
     1561 acccctgatg acacgaacga ggttctggct ttccagtgcc gcaaccgtga gctctttgaa
     1621 cgctatgagc cgacgcgtcc cccctatttt ctgactccgg cgcaccagca ggccattctg
     1681 aaatgtgagt atgatctcgc acggaaatgt gcatcgatcc gtttttatgt cttcttaaaa
     1741 accaacccgc ggatcatcat cggtaccgtc tgcctgcacg atattctgcg catgccgtac
     1801 tgctgcagcg agatcggcta caagttcgac gccgcattcc agcatcaggg ctacgccagg
     1861 gaagccgtgg caaaggtttt aaacatcgct tttaccgatc tttccctgca ccgcgtgttt
     1921 gcccgcgtga tgccggaaaa tactccctct ctccggcttt tggaagtcat cggcttcact
     1981 tgtgagggca ccgaatacgc ctgcacccag attcagggaa aatgggaaga ccacatccgc
     2041 tatgcccttt taaatcctta ctcttcaata taacgcaccc agcagccaaa atgattgacc
     2101 ggttccctgc cttccctgcg ctcaccccct gggccggcgg caaagaattc cgggaaaccg
     2161 aaaatagccg ccatcacgcg ctcctgtctc tgatatactc ccggcacgcc aaggaaccac
     2221 cgttcctccg ccgtcctgcc aagcaccaga taccgataat tgaaaaatcc atgtagcaaa
     2281 aagctgttgt ttcccagata ccagtatttt ttcggcagac tccgcagatc ctttaattcc
     2341 agctggatgc actgcgtatc ccactcctca aacagagccg ccggtttgtg ttctctgcag
     2401 agctcctccc acacggcatt ccagtcgtat tccgggaaca gattgcgcat gggaagctca
     2461 gtggcatgaa tcgcctccat ctcctccccc gccgctgccg tctccgctct ggatccggac
     2521 ggcacatcct gcgccattcc ttttgccgcc atccggctgt tctgttccga ctgtacaggg
     2581 gctgctcccc cctgctttcc ctgtgccatc tcttgcgccg ccgtctggct gttctgttcc
     2641 gcctgcgccg cggctgcttc ccgcacttct ttttgcgccg cagtttctgc tgccgcccct
     2701 ttctctgctg tcttctcctt cagcttctcc ggctgccact cccggaatcc ggacagcccc
     2761 accttaaacg gagcaccctc gcgccagcgg ctcatcagaa tccgcccctc tccgatgaca
     2821 aaaaacaatc cctccatctc tgtaatgcca taaggagatg ctccgactct gccgcctttt
     2881 aagacaacgg cgccatcgcc gcttccgttc gcaagcttca tctcaccgac cagta
//
LOCUS       DBMDQJ010000282         2931 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12127_length_2931_cov_1.502827, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000282 DBMDQJ010000000
VERSION     DBMDQJ010000282.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12127_length_2931_cov_1.502827"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1845)
                     /locus_tag="ACZO9R_06605"
     CDS             complement(<1..1845)
                     /locus_tag="ACZO9R_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HIR0912771.1"
                     /translation="MTKLALSDEILMKIDKPARYIGNELNSVCKNKDEVSIRFAMCFP
                     DVYEIGMSHLGIQILYDMFNKRDDVWCERVYSPWPDLHKIMKEENIPLFCLESQEPVK
                     NMDFLGITIQYEMCYTNILQILDLSQIPLLAADRDDTVPIVIGGGPCTYNPEPIADFF
                     DLFYMGEGEVVYDRMLDLYQEMKRAGRSRREFLHETAKIPGIYVPSLYQVEYKEDGTI
                     ASFTPVDEEIPATVTKQLVMDMTDAVYPEKPVVPFIKATQDRVVLEIQRGCIRGCRFC
                     QAGMVYRPVREKNIERLKALATQMIEATGYEEISLSSLSSSDYSSLEELVNFLIDECR
                     ERRVNISLPSLRIDAFSLDVMSKVQDIKKSSLTFAPEAGSQRLRDVINKGLTEEVILN
                     GAQMAFEGGWNKVKFYFMLGLPTETEADMRAIAELANETAARYYDTVPKEKRNGRCQI
                     TISTSFFVPKPFTPFQWARMYPPEDYIGRAKVVNDTVKEQLNKKSIKYNWHEADVTVL
                     EGVLARGDRKIGQAILKVYEKGGIFDAWSEYFDYQRWLDAFAECGIDPDFYTMRERSM
                     DEIFPWDFIDTGVTKEFLKREWNNAMKETVTPNCRMRCSGCGVKKFGGG"
     gene            complement(1859..2161)
                     /locus_tag="ACZO9R_06610"
     CDS             complement(1859..2161)
                     /locus_tag="ACZO9R_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912772.1"
                     /translation="MTDYVERRRAYVSQVRSSFETGADAGEPFDREEPQRGRAGGQLR
                     FLVALLLFLFFFAWDDSGNTVCGVTPAKMIDMIEDNRYDTILQECDAVLAELLHTE"
     gene            complement(2190..2534)
                     /locus_tag="ACZO9R_06615"
     CDS             complement(2190..2534)
                     /locus_tag="ACZO9R_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912773.1"
                     /translation="MMFYAAAVFVLIKRLVPCGSKESPFVRIIGKYSYSILLIHWGVL
                     HYAVKQVLHVNVLSGGIVGGCLLAVLLTFALSFVGAVILDQTIIRFLTFAVSKLIDVL
                     RTCFSQKSHIVS"
     gene            complement(2565..>2931)
                     /locus_tag="ACZO9R_06620"
     CDS             complement(2565..>2931)
                     /locus_tag="ACZO9R_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR0912774.1"
                     /translation="MLQRFFIGPPVEAPHLWLVYVIIWLYVLTPFLRYLVQNIPDPVM
                     NGVMLVIFLLNAYVTYVPLLGVTPPAALSGIVNSYVGVFLLGYYLANRAGRRAENFFL
                     AAGLVSYLITCYVILFHGW"
BASE COUNT          734 a          860 c          657 g          680 t
ORIGIN      
        1 acctcctccg aacttcttca caccgcagcc cgaacagcgc attcggcagt tcggcgtcac
       61 ggtctctttc atggcattgt tccactcacg ctttaaaaat tccttggtca cgccggtatc
      121 gataaaatcc catgggaaaa tctcgtccat ggaacgctcg cgcatcgtgt aaaaatcggg
      181 atcaatgccg cactccgcaa aggcgtccag ccatctctga tagtcaaaat attcggacca
      241 tgcatcaaaa atgccaccct tttcataaac ctttaagata gcctgtccga ttttccggtc
      301 gccgcgcgcc aatacgccct ctaaaactgt cacatccgcc tcatgccagt tgtatttaat
      361 gctctttttg tttaactgtt ccttgaccgt gtcgttgacc acttttgcac gtccgatgta
      421 atcctccggc gggtacattc ttgcccactg gaacggtgta aacggcttcg ggacgaaaaa
      481 ggaggtactg atcgtgatct ggcatctgcc gttccgcttc tccttgggaa cggtgtcata
      541 atagcgtgcc gccgtctcat tggcaagctc cgcgatcgcc cgcatatccg cctctgtctc
      601 ggtcggaagt cccagcataa agtagaactt gaccttattc cagccgccct caaacgccat
      661 ctgggcgccg tttaagatga cctcctccgt cagtcccttg ttgataacgt cgcgcaggcg
      721 ctgtgatccc gcctccggtg cgaatgtcag gctgctcttt ttgatgtcct gcaccttgct
      781 catgacatcc agtgaaaacg catcgatgcg gagcgacgga agcgaaatat tcaccctgcg
      841 ctccctgcac tcgtcgatca ggaaattgac aagttcctcc agagagctgt agtccgatga
      901 acttaaggag ctgagtgaga tctcctcata cccggtcgcc tcaatcatct gtgtggcaag
      961 cgcctttagg cgctcaatat ttttctcgcg caccggacgg tacaccattc ccgcctggca
     1021 gaaacggcag ccgcggatac agccgcgctg aatctcaagc acaacgcgat cctgcgtggc
     1081 tttgataaac ggtacgaccg gcttttccgg atagaccgca tccgtcatat ccatcacgag
     1141 ctgcttggtc accgttgccg ggatttcctc atccaccggt gtaaacgacg cgatcgttcc
     1201 gtcttcttta tattccacct gatacaggga aggcacatag attcccggaa tctttgccgt
     1261 ctcatgcaaa aactccctgc ggcttctgcc cgcccgcttc atctcctgat agagatctaa
     1321 catgcggtca tatacgacct ccccttctcc catataaaac agatcgaaaa agtctgcaat
     1381 tggttccgga ttgtaggtgc agggaccgcc gccgatcacg atcggaacgg tatcgtcacg
     1441 gtctgcggca agcagcggaa tctggcttaa gtcaagaatc tggagaatat tggtataaca
     1501 catctcatac tggatggtaa ttccaagaaa atccatattc ttcaccggct cctgtgattc
     1561 caggcaaaac agcgggatgt tctcctcctt catgatcttg tgcagatccg gccacgggga
     1621 atacacgcgc tcacaccaca catcgtctct cttattaaac atatcataga gaatctggat
     1681 gcccagatgg gacataccga tctcatatac atccggaaag cacatggcaa aacgaatcga
     1741 tacctcgtct ttgtttttac agacgctgtt caactcgttt ccaatgtagc gcgccggctt
     1801 atcaatcttc atcagtatct catcggataa cgctaacttt gtcatttctt ttatcctttc
     1861 attctgtatg cagaagttct gcaagcactg cgtcgcactc ctgcaaaatt gtatcatacc
     1921 gattatcttc gatcatgtca atcattttcg ccggagtgac gccgcaaacc gtatttccac
     1981 tgtcatccca tgcaaaaaag aacaaaaaca gcagcagtgc cacaagaaag cggagctgtc
     2041 cccccgcgcg ccctctctgt ggctcctctc tgtcaaaagg ctctcccgca tccgctcccg
     2101 tctcaaagga actgcggacc tgtgagacat acgctctccg ccgttctaca tagtctgtca
     2161 tactgcacct ctcctatccc gcctgtgctc taggatacta tatgagattt ttgggaaaaa
     2221 catgtgcgca gaacatcgat cagttttgaa accgcaaacg tcaaaaaacg gatgatggtc
     2281 tgatccaaaa tgacggctcc cacaaatgac agtgcaaacg tgagaagtac ggcaagcaga
     2341 cagcctccca cgatgccgcc ggacaggaca ttgacgtgaa gcacctgctt gacggcatag
     2401 tggagcacgc cccagtgaat cagcagaatc gaataactgt acttcccgat gatgcgcaca
     2461 aaggggctct ctttgctgcc gcagggcacc agccgcttta tgagcacaaa cacggcggcg
     2521 gcataaaaca tcatcgtagg cgcattgttg taaatatagt cgtcctacca gccatggaac
     2581 agaatcacat agcaggtgat cagatatgac acaagacccg ccgcaaggaa aaaattctcc
     2641 gcccgcctcc ctgcgcggtt cgccagatag tagccaagca gaaatacccc cacatacgaa
     2701 ttcacaatac cggagagtgc cgccggcggc gtaacgccaa gcagcggcac ataggtgaca
     2761 tacgcattca gaaggaagat cacaagcatc acgccgttca tgaccggatc gggaatgttc
     2821 tgcaccagat accgcaaaaa cggggtgagc acatacagcc agataatgac atagaccagc
     2881 cacagatgcg gcgcttccac cggcggtccg atgaagaacc gctgcagcat c
//
LOCUS       DBMDQJ010000283         2930 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12128_length_2930_cov_1.722163, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000283 DBMDQJ010000000
VERSION     DBMDQJ010000283.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12128_length_2930_cov_1.722163"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            183..707
                     /locus_tag="ACZO9R_06625"
     CDS             183..707
                     /locus_tag="ACZO9R_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HIR0912775.1"
                     /translation="MLERFYPGEYVDSTYAIDFDRLYEEGYRGVIFDIDNTLVPHGAP
                     ADERACALFAHLKELGYHCMLLSNNKEPRVKMFNDAVQVSYIYKAGKPNPANYRKAME
                     QMGTDEKNTLFVGDQIFTDVYGANRTGIRTILVKPIHPKEEIQIVLKRYLEKIVLFFY
                     RRKCQKQQSAAQKR"
     gene            791..1237
                     /gene="rpiB"
                     /locus_tag="ACZO9R_06630"
     CDS             791..1237
                     /gene="rpiB"
                     /locus_tag="ACZO9R_06630"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR0912776.1"
                     /translation="MKIAIGNDHAATELKFVIMDYLKELGHEVINFGTDGTDSCNYPE
                     YGEKVGRAVVAGEADCGVLICGTGVGISIAANKVNGVRAAVCSDTATARLVKEHNNAN
                     IIAFGARIVGTELAKDIVKAYLDAEFQGGRHQTRIDMIHEIEKRNA"
     gene            1397..1954
                     /gene="efp"
                     /locus_tag="ACZO9R_06635"
     CDS             1397..1954
                     /gene="efp"
                     /locus_tag="ACZO9R_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR0912777.1"
                     /translation="MISAGDFRNGITIELEGNIYQIIEFQHVKPGKGAAFVRTKLKNI
                     KSGGVVEKTFRPTEKCPTARIDRKDYTYLYADGDLYNFMDAETYEQIALSKDDIGDAL
                     KFVKENEMVKMCSHNGSVFAVEPPLFVELQITETEPGFKGDTATGATKPAIVETGATV
                     YVPLFVEQGDVIKIDTRTGEYLSRV"
     gene            complement(2037..>2930)
                     /locus_tag="ACZO9R_06640"
     CDS             complement(2037..>2930)
                     /locus_tag="ACZO9R_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912778.1"
                     /translation="YGQKKTGVPALTQLTEQIARVGTVWLLYQIHMREQTAVPLSATM
                     WGAVMGEVASTLVSVSVAKLPEGTDTALSVKCGTRNLLAMAVPLTANRVVLTLFSSFE
                     NIMIPSRLRLFGYTASEALGVYGILTGMAMSVIMFPSVITNSVSVLLMPAISEAKAGG
                     NDALIRRAVLKTIRVCLLWGFFCTAGFLATGHFIGNVLFGNALAGTFIRTLGWICPFL
                     YLGVTLSSILHGLGYPGITFVLNLIACGIRILFVLFALPVYGIRGYLCGLLISQAVMA
                     LLSIGILLRQTHPTSRSASAK"
BASE COUNT          861 a          617 c          834 g          618 t
ORIGIN      
        1 gaaactttgt tcctattaca caaaaacgct ctgcggggat gcgcacctcg cggtgaggta
       61 aataatgctc tgagaaaaac gaacaacgcg aattggaaat tccgggtgga tattttggta
      121 gaaatgtagt aatctatgta aggatgcgca ctaagtgaga tgagtaaagg agcaaaaggg
      181 caatgctgga acggttttac ccgggagaat acgtagattc tacctatgcg atcgattttg
      241 accgccttta tgaggaggga tatcgaggcg ttatttttga tattgataat acgctggtgc
      301 cgcacggcgc gccggcggac gagcgggcgt gcgcgctttt tgcacatttg aaggagctgg
      361 gatatcattg catgctgctg tcgaacaata aggaaccgcg tgtgaaaatg ttcaatgacg
      421 ctgtgcaggt ttcttatatc tacaaggcag gaaagccgaa tccggcgaat tacagaaaag
      481 cgatggaaca gatgggaacg gatgagaaaa acacattgtt tgtgggagat cagattttca
      541 cggatgtcta cggcgccaac cgcacgggaa tccgcacgat tctggtaaag ccgatccatc
      601 cgaaggagga gatccagatt gtcttaaagc gttatctgga aaagatcgtc ctgtttttct
      661 atcggagaaa atgccaaaag cagcagagcg cagcgcagaa gcgataaaag aagcagtttg
      721 tgctgcagaa aacagtggca gcatgtgaca atgaaaagga cgggtgccaa aacccgggat
      781 ggaggaaaaa atgaaaatag caatcggaaa tgatcacgcg gcaacagagt taaaatttgt
      841 cattatggac tatttaaagg aactgggaca tgaggttatc aactttggaa ccgacggaac
      901 ggacagctgc aattatccgg aatacggaga gaaagtcggc agggcagttg tggcaggcga
      961 ggctgactgc ggcgtactga tctgcggaac cggtgtggga atctccatcg cagccaataa
     1021 agtcaacggt gtgcgtgcag cagtgtgctc cgacacggca actgcgcggc tggtaaaaga
     1081 acacaacaat gccaacatta ttgcattcgg tgctagaatc gtcggcacag agcttgcgaa
     1141 agacatcgtc aaggcatatc tggatgcaga attccagggc ggaagacacc agaccagaat
     1201 cgatatgatc catgagattg agaagcgcaa cgcttaataa taccggggag attgcgccag
     1261 gaacagagca aaaagcgtgc aaattcgcat ggcgcagtgc ggtatatgaa aaaaagcttg
     1321 aaatatagcg ctgtttatta taaaatagaa aaatcagaga gtaaaactcg agcgtatttt
     1381 taaggaggaa atttacatga tttcagcagg cgatttcaga aatggtatta ccattgagtt
     1441 agagggtaat atctatcaga ttatcgaatt ccagcatgtg aaaccaggaa aaggtgcagc
     1501 atttgtaaga actaagttaa agaatattaa gagtgggggt gtggttgaga agacattccg
     1561 tcctacagag aaatgtccga ctgcccgcat tgacagaaaa gattacactt atctgtatgc
     1621 agatggtgac ttatacaact ttatggatgc agagacttat gagcagatcg cactctccaa
     1681 agatgacatc ggcgatgcgc tgaagttcgt gaaggagaac gagatggtga agatgtgctc
     1741 tcacaacgga agcgtattcg cagtagagcc gcctctgttc gtagagcttc agatcactga
     1801 gactgagccg ggcttcaagg gtgataccgc tactggtgca accaagccgg caatcgtcga
     1861 gacaggagct accgtatatg tacctctgtt cgtagagcag ggcgatgtga tcaagatcga
     1921 tacacgtaca ggtgagtatc tgtccagagt ataatttgaa atcagattta gcacttttaa
     1981 tgagaataaa aataccggta aaccgtaatc tggcttgccg gtattttttg tgcatattat
     2041 tttgcggatg cagagcggga agtgggatgt gtctggcgca gcagaattcc gatggagagg
     2101 agagccatga cggcctgact gatgagaagt ccacacagat agccgcggat accgtagacg
     2161 gggagggcaa acagcacaaa gaggatgcgg atgccgcagg caatcagatt cagcacgaaa
     2221 gtgatgcccg ggtagccgag cccgtgcaga atactgctca gcgtcacgcc gaggtacagg
     2281 aaaggacaga tccagccgag cgtccgtata aatgttccgg cgagcgcgtt cccaaaaagc
     2341 acattcccga taaagtgacc ggtagcaaga aaacccgctg tacaaaaaaa gccccacaga
     2401 agacagaccc ggatggtttt taagaccgcg cgccgtatga gcgcatcgtt tccgccggct
     2461 ttggcttctg agatggcggg cataagaagc accgaaacgg agttggtaat gacggacgga
     2521 aacatgatga cggacatcgc catgccggtt aagatgccgt agacgccaag cgcctcggaa
     2581 gccgtgtaac cgaacaggcg caggcggctg ggaatcatga tgttttcaaa actggaaaag
     2641 agcgttaaaa caacccggtt tgccgtgagc ggaaccgcca tggcaaggag attgcgcgtg
     2701 ccgcatttca cggaaagcgc cgtgtctgtt ccctccggaa gttttgcgac gctgacggag
     2761 acgagcgtgc ttgccacttc gcccatcaca gctccccaca ttgtggcgga gagcggaacg
     2821 gcggtctgct cccgcatatg gatctgatag agcagccaga ccgtgccgac acgggcgatc
     2881 tgctctgtga gctgtgtgag agcagggacg cccgtctttt tctgtccata
//
LOCUS       DBMDQJ010000284         2921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12174_length_2921_cov_1.697872, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000284 DBMDQJ010000000
VERSION     DBMDQJ010000284.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12174_length_2921_cov_1.697872"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="ACZO9R_06645"
     CDS             <1..527
                     /locus_tag="ACZO9R_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR0912779.1"
                     /translation="LTEEMPENSIGDVTWDDTCENSHAINVLGIPMVVMGAKNMVIAA
                     SHDGILVADKHQSSYIKDCLSHISDTSKYEERRWGTIKTIDNDEDNGIKSVTKRIKVV
                     AGKRTPESRHMNHTQTITVLSGMGKLILEGVEVDLMAGATVSIAAGKTYSIQALGSDL
                     RCIEISISKSKENA"
     gene            679..1053
                     /locus_tag="ACZO9R_06650"
     CDS             679..1053
                     /locus_tag="ACZO9R_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR0912780.1"
                     /translation="MDTNDISACEKLVMKVIWDSTEELALQEVMDGVNHENGKNWKPQ
                     TVSTFLSRLVKKGFLNMYRKGRYCYYQPLVSKEDFWRATMEESAKFFTKGDLGELACC
                     LCDDMLDAKDIEKLKKKLNEHS"
     gene            1040..2446
                     /locus_tag="ACZO9R_06655"
     CDS             1040..2446
                     /locus_tag="ACZO9R_06655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017390.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR0912781.1"
                     /translation="MNIANQILFAVVITSVTGTAAYGLWRLLMNSRFCTKPQYVYLAL
                     FLVCLLYLIPLDYGWMQMTIHGYLQKEGIWQINFSLTGFLWILAFFAAVCWLALMAKG
                     ITEFAGSLRGMKRIQMRDIPEEEEAVREEFARVKKKLGIRSNIRLYRNDRITSPMLAG
                     IFRCRVVLPDIAYDRKHLSVIFHHELMHYKSHDPFVRLCGMCVTAVHHINPLTGNLMA
                     LLDEWSEIYCDARAIAAISDEMDAGCYFEMIIESVKHSPVTDENYIFSMLYENQRRLE
                     RRIEYMGKYNGKAASRLASMCFAAAFVLMSVTPIYAAGSEAADVHDDIYQNVEAVTAD
                     GSEENVLEEQQILGVMDRSYDTLEYAYPELEVISPLLSANEVASFNWSVSPGTRMVSN
                     QFWVTKGQTIHMAAVATPDGNTFWIGIMDGLNNARYVEGTGNLSHAFSITSSGHYRVF
                     VQNRSSVQINAAGSYYFN"
     gene            complement(2621..>2921)
                     /locus_tag="ACZO9R_06660"
     CDS             complement(2621..>2921)
                     /locus_tag="ACZO9R_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR0912782.1"
                     /translation="SVNSSMLFCSDIKYDMNDSLLVACKDQLACDYVQTGHHGNWSFS
                     EEFYDAAGASVYFIDAPSSITDNADFPASTLKSDLLAKGKTVLDFSTAPNTVTLK"
BASE COUNT          795 a          591 c          834 g          701 t
ORIGIN      
        1 cactgaccga ggagatgccg gagaacagca tcggtgatgt gacatgggat gatacctgtg
       61 agaacagcca tgcgatcaat gtactgggaa ttccgatggt tgtgatgggc gcgaaaaata
      121 tggtcatcgc ggcatcccat gacggtattc tggtggcaga caagcaccag agctcctata
      181 ttaaggattg tctctcccat atctcggata catcgaagta tgaggaacgc cgctggggaa
      241 cgatcaagac gatcgacaac gacgaggata acggcatcaa gagcgtgaca aagcgcatca
      301 aggttgttgc cggcaagaga acgccggaga gcagacacat gaatcacacc cagacgatca
      361 ccgttctctc tggaatgggc aagctgatct tagagggtgt ggaagtggat ctgatggcag
      421 gggcaaccgt gagcattgcc gcaggaaaga cctattccat tcaggcactc ggatctgatc
      481 tgcgctgtat cgagatctcc atcagcaaga gcaaggagaa cgcataatat ctaatatttt
      541 ataaagaatg tgcttgtttt gcttgccttt ttggtaaaat tgtgataaaa atggaaaaga
      601 tacggaagta tcagaaaaaa agattgaatt tgatacgaat atggtttata ctataaccgt
      661 agaaagggga atgattttat ggatacgaac gatatttcag catgcgaaaa gctcgtgatg
      721 aaggtcattt gggattctac agaagaattg gcattgcagg aagtcatgga tggagtcaat
      781 catgagaacg gcaagaactg gaagccgcag actgtttcca catttttatc ccgtctggta
      841 aagaaaggct ttctgaatat gtaccgcaag ggcagatact gctattatca gccgcttgtc
      901 agcaaggaag acttctggcg cgcgacgatg gaagagagtg caaagttctt tacgaaggga
      961 gatctcggag aactggcatg ctgtctgtgt gatgacatgc tggatgcaaa ggacatcgag
     1021 aaactaaaga aaaagctgaa tgaacatagc taatcagatt ttatttgcag tggtaattac
     1081 ctctgtcacg ggaacggcag cgtacggact ctggcggctg cttatgaaca gccgcttttg
     1141 tacgaagccg caatatgtat atctggcgct ttttctggtc tgcctgttgt acctgattcc
     1201 attggattat ggatggatgc agatgacgat tcacggttat ctgcagaagg aaggtatctg
     1261 gcagatcaat ttttctttaa cgggattttt atggattctt gcgttctttg ccgcagtctg
     1321 ctggcttgcc ctcatggcaa agggaatcac agagtttgca ggcagcctgc gcgggatgaa
     1381 gcggattcag atgcgtgaca ttccggagga agaggaagcc gtgcgggaag agttcgcacg
     1441 tgtgaagaaa aagcttggca tccggtcgaa cattcgtctg taccggaatg accgtatcac
     1501 cagtccgatg cttgccggta ttttccggtg ccgggtggtg cttccggata ttgcctacga
     1561 cagaaagcat ctgagcgtga tctttcatca cgagctgatg cattacaaga gtcatgaccc
     1621 gtttgtcaga ttatgcggta tgtgtgtgac tgcagtgcac cacatcaacc cgctgacggg
     1681 gaacctgatg gcactgctgg acgagtggag cgagatttac tgcgatgcac gtgcgattgc
     1741 agccatcagc gatgaaatgg atgcagggtg ttattttgaa atgattatag agagtgtgaa
     1801 gcacagtcct gtgaccgatg agaattatat tttttctatg ctttatgaga accagagaag
     1861 actggaaagg agaattgagt atatgggaaa atataatgga aaagcagcat cgagactggc
     1921 aagtatgtgt tttgcggcgg cgtttgtgct gatgagtgtg acaccgatct atgcggcggg
     1981 cagcgaggct gcggatgtac acgatgatat ctaccagaat gtggaggctg tgacggcaga
     2041 tggcagcgag gagaacgtgc tcgaggagca gcagatctta ggtgtgatgg accgctccta
     2101 cgataccttg gagtatgcgt atccggagct tgaggtcatc agcccgctgc tgagcgcgaa
     2161 tgaggtggcg tcctttaact ggagcgtgtc tccgggcacg cgcatggtga gcaaccagtt
     2221 ctgggtgacc aagggacaga cgatccatat ggcggcagta gcaacgccag atggtaatac
     2281 gttttggatt ggcattatgg atggtctgaa caatgcccgc tacgtggagg gaaccggcaa
     2341 tttatctcac gctttttcga tcacgagcag cggtcattac cgcgtcttcg tgcagaacag
     2401 gagcagcgta cagatcaacg cagcaggaag ttattacttc aactgacagc agcgtaccgc
     2461 gatcagagca tcggaagagg tgcatcggaa gatgcgaaag cggtaaagac aacaaaagca
     2521 ataaaaaaga agaggcattc cgcctcttgc ttttcagaca aacaaactac atgattagat
     2581 ataattccac ggttgcagtg catttcacat tatgattaga tcatttcaag gtaacagtat
     2641 tcggggcggt gctaaaatca agcaccgtct ttccttttgc gagcaggtcg gatttgagtg
     2701 tgctcgccgg aaagtcggcg ttgtcggtga tggaggaagg cgcatcgata aaatagacgg
     2761 aggcgccggc tgcgtcgtag aattcctccg agaaagacca gtttccgtgg tgaccggtct
     2821 gcacgtaatc gcaggcgagc tggtctttgc atgcgacaag cagggaatca ttcatatcgt
     2881 atttgatgtc cgaacaaaag agcatgctgc tgtttacgct g
//
LOCUS       DBMDQJ010000285         2902 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12274_length_2902_cov_1.688326, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000285 DBMDQJ010000000
VERSION     DBMDQJ010000285.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12274_length_2902_cov_1.688326"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..109
                     /gene="rpsI"
                     /locus_tag="ACZO9R_06665"
     CDS             <1..109
                     /gene="rpsI"
                     /locus_tag="ACZO9R_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007590784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR0912783.1"
                     /translation="STLKKAGFLTRDPRMKERKKYGLKAARRAPQFSKR"
     gene            735..1265
                     /locus_tag="ACZO9R_06670"
     CDS             735..1265
                     /locus_tag="ACZO9R_06670"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0912784.1"
                     /translation="MYGLIRPVVKGKTVLELATGTGLIAKNIVNAAAHIEASDTSAEM
                     IREAKRRNHSAKLYFSVQDMFCLPYADESFEVVIVSNALHIVPQPEKALAEIHRVLRD
                     DGVLIAPTFTHAENSFFGNRKAFFLKLAGFPLHSRWTSEEYLCFLQQNGWTVRKSDVL
                     KASFPLTYVECVKAEV"
     gene            1267..1872
                     /locus_tag="ACZO9R_06675"
     CDS             1267..1872
                     /locus_tag="ACZO9R_06675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0912785.1"
                     /translation="MSFFENTRKPVGFGGKIMVAMMNLGHSPVARWGLRFLELAPDAR
                     VLDCGCGGGANIKRLLKKCPQGIVRGVDYSAVSVEKARNLNRTAIQKGRCAVWQGSVE
                     RIIFAKDWFDAVTAFETVYFWSDLPRCLREVRRVLKPGGTFLICNESSGDTDKDEKWT
                     EIIGGMTIYKGSQLKAFLEQAGFQNIQIHKRKNWLCVTAQK"
     gene            1940..2623
                     /locus_tag="ACZO9R_06680"
     CDS             1940..2623
                     /locus_tag="ACZO9R_06680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0912786.1"
                     /translation="MERKEAIRGAYRLTGGSNFYDGMITCSTFSGKAVCRLVWDMNKA
                     ENDAYLEKAMAGIPENFSGKLLEVPVGTGILTMPLYQTLPQADITCLDYSSDMMGQAQ
                     EKAERLHLENVTFRQGDVGALPFADGAFDIVLSLNGFHAFPDKEAAYREVFRVLKSGG
                     TFCGCFYVAGECKQSDWFVRHIYEKTGFFTPPYETVSSLKARLEGMYTDVDRGNLKSM
                     AWFVCRKAE"
     gene            2665..>2902
                     /locus_tag="ACZO9R_06685"
     CDS             2665..>2902
                     /locus_tag="ACZO9R_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0912787.1"
                     /translation="MKYKIEKNTVQETLILPLYSRKLCTELYPNLYRDETAVRLMDEI
                     DYDFSEAEKNSRSLMQRFGALEVAMRQNDLAFEVQ"
BASE COUNT          750 a          648 c          855 g          649 t
ORIGIN      
        1 ctccaccctg aagaaggccg gcttcctgac ccgcgatcct cgtatgaagg aacgtaagaa
       61 gtacggcttg aaggctgccc gtcgcgctcc gcagttcagc aagcgctgat tatcagtact
      121 acttgcgaac ttttcagaac gcttcacaac ttttttgcac gactgtgtgc ggaaagttgg
      181 aagtgtctga agccgtggaa tgcagcaaat cgccttgggg cttgctgaaa aagcagcata
      241 caataagcga atatgcagca gacttccaca gcgacataag ccttatcgca ggagatgttt
      301 tccgcaagga aagcatctcc tgtttttttg tttgagaaaa aagcctcatc agaatattgc
      361 gcaaagacta ttgcaaaaaa gaagtaagcg cggtacaata tagaaataaa gataaatgcc
      421 gctgcaacgg tgcaaaaagg accccggacg tttcccgaaa ggggaaaatc gagtggaact
      481 tatcctcggt gaataaaaga cgctgatagg cactgcctgc agtaagttat ttgaataagc
      541 aaaaaggccg cagaaatgcg gcttttctta agtggatagg ttagttaatt ctaacccaga
      601 agcgtatgcg tatcgtttgc tgaatcttac atcgaggagg aaggattaca atgcgagaac
      661 ataagaactt ttgggacaga aatgcaggtc ggtatgaccg ctttatgcga aaagacaggg
      721 cggcatacga aacaatgtat ggactgatcc gtccggtcgt gaaaggcaaa acagtgctgg
      781 agcttgccac cggcacgggt ctgattgcta aaaacatcgt aaacgcggcg gcgcatatcg
      841 aggcaagcga tacctctgcc gaaatgattc gggaggcaaa acgcaggaat cactctgcca
      901 agctgtactt ttcggtgcag gatatgttct gtctgcccta tgcggacgag tccttcgagg
      961 tggtgatcgt gtccaacgcc ctgcacatcg taccgcagcc ggagaaagcc cttgccgaga
     1021 ttcatcgggt actgagggat gacggcgtgt tgatcgcgcc taccttcacc cacgcagaaa
     1081 actccttttt cggaaacagg aaggcttttt ttctgaagct ggcgggcttt cccctacaca
     1141 gcaggtggac gagtgaggaa tacctgtgtt tcctgcagca aaatggttgg actgtgcgga
     1201 aaagcgatgt actaaaggcg tcttttccgc tgacttatgt tgaatgcgtg aaagcggagg
     1261 tgtgatatgt cgttctttga aaacacccgc aagcctgtgg gctttggcgg gaaaattatg
     1321 gttgctatga tgaatctggg acacagtccc gtggcgcggt gggggcttcg ctttttagag
     1381 cttgcgccgg atgccagagt gctggattgc ggctgcggag gaggcgccaa tatcaaaagg
     1441 ctgctgaaaa aatgcccgca aggcatcgtc agaggtgtgg attattctgc tgtcagcgta
     1501 gagaaagccc ggaatttaaa ccggactgcc attcagaaag ggcgctgcgc ggtgtggcag
     1561 ggcagcgtgg aacgtatcat ctttgccaag gattggtttg atgcggttac cgcgtttgaa
     1621 acggtctatt tctggtcgga tttgccgcgg tgccttcggg aagtccggcg ggtgttgaag
     1681 cccggtggga catttctcat ctgcaatgag agcagcggtg acacggacaa ggacgaaaag
     1741 tggacggaga tcatcggcgg catgaccatc tacaagggta gccaactgaa agcgttttta
     1801 gagcaggcag gttttcaaaa tattcagata cacaaaagaa aaaactggct ttgcgtcacg
     1861 gcacagaaat aggatgcagc ggtgaaccat ccggcatcag aagggcgatt gtaatgcgga
     1921 aagatgagga ggaaccccta tggagcgaaa agaagcgatc cgcggcgcct atcggctgac
     1981 aggcggcagc aatttttacg acggcatgat cacctgctca acctttagcg gaaaagcggt
     2041 atgccgcttg gtgtgggata tgaacaaggc ggaaaacgac gcctatctgg aaaaggcaat
     2101 ggcaggtatt cctgaaaact tctcgggtaa gctgctggag gtgccggtgg ggaccggcat
     2161 tctgaccatg ccgctttacc aaacgctgcc ccaagcggat attacctgcc tcgattactc
     2221 gtcggacatg atggggcagg cgcaggaaaa ggcggaacgt ctgcacctgg aaaatgtgac
     2281 cttccggcag ggggatgtag gggcgctgcc ctttgcggac ggtgcgtttg atattgtgtt
     2341 gtcgctgaat ggctttcacg ctttcccgga caaagaggct gcttatcggg aggtctttcg
     2401 ggtgctgaag tccggcggca ccttctgcgg ctgcttttat gtggcgggag agtgcaagca
     2461 gtcggattgg tttgtccggc acatatatga aaagacgggg ttcttcacgc cgccctatga
     2521 gacggtttcc agcctgaagg cgcggctgga aggtatgtat acggatgtgg ataggggaaa
     2581 tctgaaaagc atggcatggt ttgtctgccg gaaagcagaa taacaggcac gaagctgccc
     2641 tttatcagaa agtgagctgt ctttatgaaa tacaaaattg agaaaaacac cgtgcaggag
     2701 acgctgatcc tcccgctgta ttcccggaag ctgtgcaccg agctgtatcc gaacctttac
     2761 cgggacgaaa cagcggtgcg actgatggat gaaatcgact atgacttttc agaggcagag
     2821 aaaaattccc gcagcctgat gcagcgtttc ggtgcgctgg aggtcgccat gcggcagaat
     2881 gaccttgcct ttgaagtgca gg
//
LOCUS       DBMDQJ010000286         2898 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12301_length_2898_cov_1.308902, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000286 DBMDQJ010000000
VERSION     DBMDQJ010000286.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2898)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12301_length_2898_cov_1.308902"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2084)
                     /locus_tag="ACZO9R_06690"
     CDS             complement(<1..2084)
                     /locus_tag="ACZO9R_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912788.1"
                     /translation="MKKMRQTLALLLAGALTVGSFAGCGGSKSDSETAGTETAGTEAA
                     GTTASGSDTPLVIANDGMSEKFSPFFAESVPDQHIVDVTQISLVYNDRSGEFIYNGIE
                     GETTSYNGTDYTYYGPTDLTITENEDGTVYYDFKLRDDLTFSDGEPVTADDIIFSFYV
                     FCDPTYDGSASVYSLPIEGMEEYRSGMSTLASLLAAAGEDNTEFTYWTEDQQNAFWDA
                     VNDGGAAFAQEIVDYCVENGVSEEGDVAGAAAQWGFDGLAADATAKDFFMAIGDKYGW
                     SFTAMEAESAGSALSDLIPEDVYAYATEGVETGDAAANISGIQKLDDKTVRVVLTEVS
                     APALQTMDIQIAPLHYYGDESQYDYDNNQFGFTKGDLSAIREKTTAPLGAGPYVFKSY
                     ENKTVYLEANESYYKGAPVTKELQFKETAEADKLPGVVQGTVDISDPSISKEVMAQIC
                     SENSNGEASGDVLTTALYDNNGYGYIGINSQNVKVGDDPSSEQSKDLRKAIATVISVY
                     RDMVIDSYYGDAASVINYPISNTSWAAPQKSDADYAVAFSKDVDGNPIYTDGMSDDEK
                     YAAALDAALGFFEAAGYTVTDGKLTAAPAGAKLSYEVMIGGGGKGDHPSFGILTAASE
                     ALKSIGFDLTINDLADGTIMWDALNAGTAEMWAAAWQATVDPDMYQVYHSASVKSNYY
                     HINDETLDQDII"
     gene            complement(2142..2702)
                     /locus_tag="ACZO9R_06695"
     CDS             complement(2142..2702)
                     /locus_tag="ACZO9R_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912789.1"
                     /translation="MIKNPYQKDYQNELKQNRHGLLVTRTSYQGDFYVLPFDEQQKRR
                     TGILNVIWTIALWVIELGMGLLNPDSSRTAWIVFPYLFVILPLGYMLYGAVSYIGAPL
                     RMHRAHYETGLLRMKRSCIGAMVLTGIGAVLDLVYMVLHRGGIRMGIECIYLIGHFLF
                     LAVAFSFGKYYDRNYSGILIENATME"
BASE COUNT          717 a          775 c          633 g          773 t
ORIGIN      
        1 tcaataatat cctgatccag tgtttcatca ttgatgtggt aataattgct ctttacactt
       61 gcgctgtggt aaacctgata catatcagga tccacagttg cctgccatgc agctgcccac
      121 atttctgctg tacctgcatt taaagcatcc cacatgatgg ttccgtcagc caggtcgttg
      181 attgtcagat caaatccgat gctcttgagt gcctcggatg ctgcggtcag gataccgaag
      241 gacggatgat ctccctttcc gcctccgccg atcattacct catagcttaa cttcgcgccg
      301 gccggagctg ctgtcagctt gccgtctgtt actgtgtaac cagctgcctc gaagaatccg
      361 agtgctgcgt caagtgctgc tgcatacttc tcgtcatcag acataccgtc tgtgtagatc
      421 gggttgccgt ctacatcctt ggagaatgca accgcatagt ctgcatcaga cttctgcggt
      481 gctgcccagg aagtattgga aatcgggtag ttgattacag atgcagcgtc tccgtagtaa
      541 gaatcgatca ccatgtcacg atatacggaa ataacagttg cgattgcttt tcttaaatct
      601 ttggactgct cagaagaagg atcatcgcca accttgacat tctgagagtt gattccgatg
      661 tagccataac cattgttgtc atacagagct gtggttaata catcaccgga tgcctcaccg
      721 ttgctgttct cggagcagat ctgagccata acctctttgg agatggacgg atcagaaata
      781 tctactgtac cctgaacaac accaggaagc ttatctgcct cagcggtctc cttgaactgg
      841 agctccttgg taaccggtgc tcccttgtag tatgactcgt tcgcctcgag atatactgtc
      901 ttgttctcgt agctcttgaa gacatacgga ccagcgccaa gcggagcggt tgtcttctcg
      961 cggattgcgg aaagatcacc ctttgtgaaa ccaaactggt tgttatcgta atcgtactgg
     1021 ctctcatcac catagtaatg aagcggtgcg atctggatgt ccatagtctg aagtgccggt
     1081 gcgctgacct ctgttaaaac aacgcggaca gtcttgtcat ccagcttctg gataccggaa
     1141 atgtttgctg ctgcatcacc ggtctcaaca ccctcggtag cgtatgcata tacgtcttcc
     1201 ggaatcagat cggagagagc gcttcctgcg gactcagcct ccatagctgt aaagctccat
     1261 ccgtacttat caccgattgc cataaagaaa tccttagcag tcgcatctgc tgcaagaccg
     1321 tcgaagcccc actgtgctgc tgcgcctgct acgtcgccct cttcgctgac gccgttctct
     1381 acgcagtaat caacgatctc ctgtgcaaat gctgcaccac cgtcatttac agcatcccag
     1441 aatgcgttct gctgatcttc tgtccagtat gtgaattctg tgttatcctc gcctgctgct
     1501 gcaagtaaag atgccagtgt ggacataccg cttctgtact cttccatacc ctcgatcggg
     1561 agagagtata cagatgcgct tccgtcataa gtcggatcgc agaatacata gaaagagaag
     1621 atgatatcat ctgcggttac cggctctcca tcggagaaag tcagatcatc acgaagcttg
     1681 aaatcatagt atacggttcc gtcttcgttc tctgtgatgg tcagatcggt cggtccgtag
     1741 taagtataat cggtaccgtt gtatgaagtg gtctcaccct cgataccatt gtagataaac
     1801 tcaccggaac gatcgttgta aaccagagaa atctgtgtca catcaacgat gtgctgatcc
     1861 ggcacactct cagcgaagaa tgggctgaac ttctcgctca taccgtcatt tgcgataacg
     1921 agcggtgtgt cgctgccgga tgcagtcgta cctgctgcct ctgtgcctgc tgtctcggtt
     1981 ccagctgtct cactgtcaga cttgcttccg ccgcagcctg caaatgaacc gacagtcagt
     2041 gctcctgcca gtaacaaagc aagagtctgt ctcatttttt tcataatatt tcctccttat
     2101 aaaaatatta tctatgtttt aaccttataa aaggaaaaaa cctactccat tgtggcattc
     2161 tcaatcagaa tgccgctgta attgcggtca taatactttc cgaaggaaaa tgcaaccgcc
     2221 agaaacagga aatgccctat cagatagata cattcgatcc ccatacggat cccaccacgg
     2281 tgcagcacca tgtatacaag atccagaacc gctccgattc ccgtcaatac cattgccccg
     2341 atgcaggaac gcttcatacg aagcagccca gtctcataat gtgcccggtg cattcggagc
     2401 ggtgctccga tatagctgac cgcaccataa agcatgtatc caagcggaag tatcacaaac
     2461 agatacggaa atacaatcca tgccgtgcgg gacgaatccg ggtttaaaag tcccattcca
     2521 agctcaatca cccacagcgc gattgtccag atgacattta aaatgcctgt ccgccgcttc
     2581 tgctgctcat caaacggaag cacataaaaa tctccctgat aggacgttct tgtcaccaga
     2641 agaccatgcc ggttctgctt taattcattt tgataatcct tttggtaagg gttcttgatc
     2701 atcgccgctt ccgcctcatc gctctctgtc tgtcatgcag catagtgcaa aattaccgcg
     2761 ttattgtgct aaaatatttt tgtgtaacaa aaaaaagtga caatgaacct ctttgtccac
     2821 tgaacacttc acaaaataca cttgtatgtt gcacacgcac agcggccctg cgcgccatga
     2881 atttcccgat ctcctcaa
//
LOCUS       DBMDQJ010000287         2893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12317_length_2893_cov_1.385745, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000287 DBMDQJ010000000
VERSION     DBMDQJ010000287.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12317_length_2893_cov_1.385745"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            17..1648
                     /locus_tag="ACZO9R_06700"
     CDS             17..1648
                     /locus_tag="ACZO9R_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016852 - sirohydrochlorin
                     cobaltochelatase activity [Evidence IEA];
                     GO_process: GO:0019251 - anaerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin cobaltochelatase"
                     /protein_id="HIR0912790.1"
                     /translation="MKKKTLALLLSLTLAAGMIGGCGSKTETTTEENVQTAQTETVAE
                     TGTEAVSDQDAADHVAALIDAIYVQERTDETDAQCAEARAAWDALTDAQKELVEGENA
                     DPDYFGKDTGDASKDDPLNEDEIGENEILVVSFGTSFNDSRTEDIGGVEKAIAEAYPD
                     WSVRRAFTAQIIINHVQARDDEKIDNVDQALDRAVANGVKNLVIQPTHLMHGAEYDEL
                     MESVEKYRDQFTSVAVAEPLLGEVGEDATVINDDKEAVAKAITAEAVAEAGYDSLDAA
                     AEDGTAFVFMGHGTAHTAKVSYSQMASVMKNLNYANVFIGTVEGEPEETACEAVIEAV
                     KEAGYTKVVLRPLMVVAGDHANNDMAGDDDDSWKSMFEADGSFESVTCQIAGLGRIDT
                     IEQLYVSHTQDAIDSLGSVDTAAAATTAGNLEDGTYSAVFDTDSSMFHVNEACDGRGT
                     LTVKDGKMTIHVSLVSQSIVNLYPGTAEDAQKSGAKLLEPTKDTVTYSDGTSEEVYGF
                     DIPVEALDQEFDLAIIGTKGVWYDHKVSVSDPVAE"
     gene            1652..>2893
                     /locus_tag="ACZO9R_06705"
     CDS             1652..>2893
                     /locus_tag="ACZO9R_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912791.1"
                     /translation="MKKNVTELIVCLLSVICFLTACHTPQNEKLADGTYQADVTLTGG
                     SGRATVDSPAAITVKDGKIYATIVWSSPYYDYMMVEDETCYNEAEAGENSTFTIPIEK
                     LPCEISVIADTTAMSVPHEIEYTLSFALKETDFSELTKTGQWDLSYATQYRVEQYGEY
                     DLITMADDSRFLLVPENVPVPEHVPEDVVVLKQPLDHTYLVSSSVMDMIREIGALSDI
                     RLTGVKESDWYIEKAAAAMEDGRMLYAGKYNEPDYELILKEGCNFALENTMIGHNPEV
                     KEKLESLGIPVMVERSSYEEHPLGRLEWIRLYGLLYGKEQEADAFYEEQLKKIEPIME
                     KEDTGRTVAFFYITANGAVNVRKPNDYIAQMIGLAGGTYVLNDRLSAEENALSTMNMQ
                     MEDFYAAAKDADILIYNSTIEG"
BASE COUNT          819 a          614 c          880 g          580 t
ORIGIN      
        1 atcagcagga ggaattatga aaaaaaagac actggcactc ttactaagcc tgacactcgc
       61 agccggcatg atcggcggat gcggcagtaa gacagagaca accacagagg agaacgtgca
      121 gacagcacag acagagactg ttgcggagac cgggacagag gcagtatccg atcaggatgc
      181 ggcagatcat gttgcagcgc tgattgatgc aatctacgta caggagcgca cggatgagac
      241 cgatgcgcag tgtgcagagg caagggcggc atgggatgca ctgaccgatg cacagaagga
      301 acttgtcgaa ggtgagaatg cagatccgga ttatttcgga aaagataccg gggacgcttc
      361 caaggacgat ccgttaaatg aggatgagat cggcgagaat gagatccttg tcgtaagttt
      421 tggtacatct ttcaatgaca gccgtaccga ggatatcggc ggcgtggaaa aagcaatcgc
      481 ggaggcatat ccggactggt cggtaagaag agcgtttact gcacagatta tcatcaacca
      541 tgtacaggcg agagatgatg agaagatcga caatgtagac caggcgttag accgcgcggt
      601 ggcaaacgga gtgaagaacc ttgtgattca gccgacccat ctgatgcatg gtgcagagta
      661 tgacgaactg atggaatcgg tagagaagta ccgcgatcag tttacatccg ttgcagtcgc
      721 agaaccgctg ctcggcgagg ttggcgaaga tgcaacagta atcaatgacg ataaggaagc
      781 cgttgcaaag gcgatcacgg cagaggcagt tgcagaggca ggttatgatt ccctggatgc
      841 ggcagctgaa gacggtaccg catttgtatt catgggacat ggaaccgctc ataccgcgaa
      901 ggtaagctac agccagatgg caagtgtgat gaagaactta aactacgcga atgtcttcat
      961 cggcactgtg gaaggagaac cggaagagac tgcatgcgag gcagtgatcg aggcagtcaa
     1021 agaagccggc tacacgaaag tggtattaag accgctcatg gtagtggcag gagatcatgc
     1081 caacaatgat atggccgggg atgacgatga ttcctggaag agcatgtttg aggcggacgg
     1141 atcttttgag agtgtaacat gccagatcgc aggtcttgga agaatcgata cgattgagca
     1201 gctgtatgta tcccacacgc aggatgcgat cgattcgctc ggcagcgtag ataccgcagc
     1261 agcggcaacc acagcaggaa atctggagga cggcacgtac agtgccgtct tcgacactga
     1321 cagcagtatg ttccatgtca atgaggcatg tgacggacgc ggcacactta ccgtcaagga
     1381 tggcaagatg accattcacg taagtcttgt gtcacagagc atcgtgaatc tgtatccggg
     1441 aacagccgag gacgcgcaga agagcggagc caagctgctt gagccgacaa aggataccgt
     1501 gacctacagt gacggaacat ccgaggaagt gtacggattc gatattccgg tggaagcact
     1561 ggatcaggaa tttgatctgg caattattgg aaccaagggt gtgtggtacg atcacaaggt
     1621 gagtgtatcc gacccggttg cggagtaaga gatgaagaag aatgtaacag aactgatcgt
     1681 atgcctgctg tctgtgatct gtttcctcac agcgtgccac acgccgcaga atgaaaaact
     1741 ggcggacgga acctatcagg cggacgttac gctcaccggc ggcagcggca gggcgacggt
     1801 ggactcaccg gcggcgatta cggtgaagga cggcaaaata tatgccacca tcgtgtggag
     1861 cagcccgtat tatgattaca tgatggtaga ggatgagacc tgctacaacg aggcagaggc
     1921 aggagagaac tcgacgttta cgattccgat tgaaaagctg ccatgtgaga tctcggtgat
     1981 tgcggatacg acggcgatga gcgtgccgca tgagattgag tacacactgt catttgcgtt
     2041 aaaagagacg gacttttcgg aactgacgaa gacggggcag tgggatctct cttatgccac
     2101 gcagtatcgt gtggaacaat acggggagta tgatctgatt acaatggctg atgacagccg
     2161 ttttttgctg gtgccggaga atgttccggt accggaacat gtgccggagg atgtggttgt
     2221 gttaaaacag ccgctggatc acacgtatct ggtatcctca tcggtgatgg atatgatccg
     2281 tgagatcgga gcgctttctg acatccggct tacaggagta aaggagtcgg actggtatat
     2341 agagaaagct gcggctgcga tggaagacgg ccgtatgctg tatgctggaa aatacaatga
     2401 accggactat gaactgattt taaaggaagg gtgcaacttt gcactggaga atacgatgat
     2461 cggacataat ccggaggtaa aagaaaagct ggagagcctt ggcataccag tcatggtgga
     2521 acgttccagc tatgaagaac atccgctcgg aagactggag tggatccggt tatatggtct
     2581 tttgtatgga aaagaacagg aagccgatgc attttatgaa gaacagctaa aaaaaataga
     2641 accaatcatg gaaaaggaag atacaggccg cacggtggcc tttttttata tcacggcgaa
     2701 cggagcggtc aatgtccgca agccgaatga ctatattgcg cagatgatcg gactcgccgg
     2761 cggcacctac gtcctaaacg accgtctttc ggcggaggag aatgccctgt ccacgatgaa
     2821 catgcagatg gaggattttt atgcggcagc gaaggacgcc gacatcctca tctacaacag
     2881 tacgatcgag ggg
//
LOCUS       DBMDQJ010000288         2890 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12333_length_2890_cov_1.550735, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000288 DBMDQJ010000000
VERSION     DBMDQJ010000288.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12333_length_2890_cov_1.550735"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="ACZO9R_06710"
     CDS             <1..441
                     /locus_tag="ACZO9R_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PstC family ABC transporter permease"
                     /protein_id="HIR0912792.1"
                     /translation="GQSILCASILLGIMILPTIIGASEPTIRAVEQSYYEGALALGAT
                     HERSVFTVVVPAAKSGILAAVILGVGRALGETMAVMMVVGNQPRVPNSILQGVRTLTT
                     NIVMEMGYATDLHREALIATGVVLFAFILIINLCFSAIKRSGKE"
     gene            445..1323
                     /gene="pstA"
                     /locus_tag="ACZO9R_06715"
     CDS             445..1323
                     /gene="pstA"
                     /locus_tag="ACZO9R_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR0912793.1"
                     /translation="MEQINPINKVTLKDKMKSYTRHPGSLILLLLVLTAAALTVVTLL
                     FLVGYILISGIPYLKPSLFALHYNSDNVSMLPAIINTVEMVVVSLLFSVPFGVGAAIY
                     MIEYARKGNKFVEVVRLTAETLTGIPSIIYGLFGMLFFNIALHWRYSMLSGAATLAIM
                     VLPTIMRTTEEALLCVPDSYREGSFGLGAGKLRTIFKIVLPAAIPGILSGIILSIGRI
                     VGETAALIYTAGTVTGVAGLMDSGRTLAIHMYVLSNEGLHKNEANATAVVLLVIVLLI
                     NGVSSLIAKRFEAVKE"
     gene            1349..2101
                     /gene="pstB"
                     /locus_tag="ACZO9R_06720"
     CDS             1349..2101
                     /gene="pstB"
                     /locus_tag="ACZO9R_06720"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR0912794.1"
                     /translation="MGEKFNISNLNLYYGDFHALKDVDLSINEKEITAFIGPSGCGKS
                     TFLKCLNRMNDLVQGCKITGQVLLDGEDIYSGMDVNLLRKRVGMVFQKPNPFPMSIYD
                     NIAYGPRTHGIRSKAKLDEIVEKSLRDAAIWDEVKDRLKKSALGMSGGQQQRLCIARA
                     LAVDPEVILMDEPTSALDPISTSKIEDLAVELKKKYTIIMVTHNMQQAVRVSDKTVFF
                     LLGEVIESGDTEKLFSVPQDKRTEDYITGRFG"
     gene            2117..2770
                     /gene="phoU"
                     /locus_tag="ACZO9R_06725"
     CDS             2117..2770
                     /gene="phoU"
                     /locus_tag="ACZO9R_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR0912795.1"
                     /translation="MRNRFERQLLELNNELIQMGSMIEHAIEMGISALVRQDVEKAKK
                     AIAYDEEIDEQEKTVESLCMKLLLQQQPVAKDLRLISAALKMITDMERIGDHAADISE
                     MTILMSDATYERSAINIDLVESMAKETTDMVIKCVDAFVNKDLELARTVIAQDDVVDD
                     LFADFKQQLIKKINENVKNGEQATDMLMVAKYFERIGDHATNIAEWVIYSITGEHEK"
BASE COUNT          734 a          657 c          843 g          656 t
ORIGIN      
        1 ggacagagta ttctctgtgc gtcgattctt ctgggaatca tgatcctgcc gaccatcatc
       61 ggagcgtccg aaccgacgat ccgcgcagtg gagcagagtt attacgaagg agcgctggcg
      121 ctcggggcaa cgcatgagag aagtgtcttt accgtcgtgg taccggcggc aaaatccggt
      181 atactggcgg cggtcatcct cggcgtcgga cgtgcactcg gagagacgat ggcggtcatg
      241 atggtcgtgg gcaaccaacc gcgtgtcccg aacagtattc tgcagggcgt ccgtacactg
      301 acgacgaaca ttgtcatgga gatgggctat gcgacggatc tgcaccgtga ggcgctgatc
      361 gctaccggcg tggtgctgtt cgcgtttatt ctgattatca atctgtgctt ctcggcaatc
      421 aagcgaagcg gaaaggagta ggggatggag cagataaatc cgatcaacaa agtaacactg
      481 aaagataaaa tgaaatccta taccagacat ccgggctctt tgatcctgct gcttctggtt
      541 ctgactgcag ccgcgctcac ggtggtgacg ctgctttttc tggtgggcta cattctcatc
      601 agcggtattc cgtacttaaa gccgagcctg tttgcgctac attataattc ggacaacgtg
      661 tccatgcttc ctgccatcat caacacggtg gagatggtcg tggtctcact gctgttttcc
      721 gtaccgtttg gcgtgggagc ggcgatctac atgatcgaat atgccagaaa gggcaataaa
      781 ttcgtggagg ttgtccggct gaccgcggag acgctgaccg gtattccttc tattatctat
      841 ggtctgttcg gtatgctgtt tttcaacatt gcactgcact ggcgctattc catgctctcc
      901 ggagcggcga cgctcgccat catggtgctg ccgacgatca tgagaaccac ggaggaggcg
      961 cttttgtgtg tgccggattc ctacagagag ggaagcttcg ggctgggcgc cggaaaactg
     1021 cggacgattt ttaagattgt gcttccggcg gcaatcccgg gaattctatc cggtatcatt
     1081 ctctcgatcg gacgtatcgt cggagagacc gcggcgctca tttacacggc gggtaccgtg
     1141 accggtgtgg cgggactcat ggattccggg cgtacgctcg ccatccatat gtatgtgctt
     1201 tctaatgaag gtttacataa gaatgaagca aatgcgactg cagtggtgct gcttgtcatt
     1261 gtccttctga taaatggagt ctcatccctg atcgcgaagc ggtttgaggc agtgaaagaa
     1321 tagcgcgaag cgcggaaatg aggaaaacat gggagaaaag tttaatatat cgaatctgaa
     1381 tctgtattat ggcgatttcc acgcgctcaa ggacgtggat ctgtccatta acgaaaaaga
     1441 aattaccgca tttatcggtc cgtccggctg tggaaaatcc acctttttaa aatgcttaaa
     1501 ccgcatgaac gacctggtac agggatgtaa gatcacagga caggtgctgc tcgacgggga
     1561 agacatctac agcggcatgg acgtgaacct gctgcgcaag cgtgtcggca tggtattcca
     1621 gaagccgaat ccgttcccga tgagcattta tgacaacatt gcatacggac cgcgcacgca
     1681 cggcatccgc tccaaggcaa agctggatga gatcgtcgaa aaatccctgc gcgatgcggc
     1741 gatctgggat gaggtgaaag accggttgaa aaaaagtgcg ctcggaatgt ccggcggaca
     1801 gcagcagcgt ctgtgcattg cgcgtgcgct ggcggtcgat ccggaagtga ttctgatgga
     1861 tgagccgacg tctgcgcttg acccgatttc aacctcgaag atcgaggatc tcgcggtaga
     1921 actgaagaaa aaatatacga tcattatggt gacgcacaac atgcagcagg cggtgcgtgt
     1981 atccgataag accgtattct tcctgctcgg cgaggtcatt gagagcggag atacggaaaa
     2041 attgttctcc gtgccgcagg ataagcggac agaggattac attaccggac gtttcggttg
     2101 atgaaaggag caagcaatga gaaacagatt tgagagacag ctgttagaac tgaacaatga
     2161 actgatccag atgggaagca tgattgagca cgcgattgag atggggatct ccgcgctggt
     2221 gcgccaggat gtggagaagg cgaagaaggc gatcgcctac gatgaggaaa ttgacgagca
     2281 ggaaaagacc gtggagtcgc tctgcatgaa gctgctgttg cagcagcagc cggtcgcaaa
     2341 ggatctgcgc ctgatctccg cggcgttaaa gatgattacc gatatggagc ggatcggaga
     2401 tcatgcggcg gatatttccg agatgacgat tctgatgtcc gacgcgacat acgagcgcag
     2461 cgcgatcaat attgatctgg tggaaagcat ggcgaaagag accaccgata tggttatcaa
     2521 atgcgtggat gcgttcgtca acaaggatct ggagctggcg cgcacggtga tagcgcagga
     2581 tgacgtggtc gatgatctgt tcgcggactt taagcagcag ctgatcaaaa agatcaatga
     2641 gaacgtcaaa aacggcgagc aggcgacgga tatgctgatg gtggcaaaat attttgagcg
     2701 tatcggcgat cacgcgacaa acattgccga gtgggtcatc tattcgatca cgggagagca
     2761 cgagaagtag gaaatcggtc taaaaaccgt gattttacac agattttaca taaagcttac
     2821 cggaaaatta cctgaaatta aaaaatttta ccgactcctt ggattgtaat tgcaaaacgt
     2881 caaaaaatac
//
LOCUS       DBMDQJ010000289         2887 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12353_length_2887_cov_1.560302, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000289 DBMDQJ010000000
VERSION     DBMDQJ010000289.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12353_length_2887_cov_1.560302"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="ACZO9R_06730"
     CDS             <1..344
                     /locus_tag="ACZO9R_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR0912796.1"
                     /translation="AERYEEQYLALIDELHNLPGHPYLYLMAPPEAFPAEEGEILYGI
                     NNDVIGGEIRNLVPDIAEQSGCGTLDLYALTQDHPEYFVDGIHPTEEGYALIAQMVAD
                     QIRADRNAGVW"
     gene            409..482
                     /locus_tag="ACZO9R_06735"
     tRNA            409..482
                     /locus_tag="ACZO9R_06735"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:443..445,aa:Pro,seq:ggg)
     gene            complement(763..848)
                     /locus_tag="ACZO9R_06740"
     tRNA            complement(763..848)
                     /locus_tag="ACZO9R_06740"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(811..813),aa:Ser,seq:cga)
     gene            995..1273
                     /locus_tag="ACZO9R_06745"
     CDS             995..1273
                     /locus_tag="ACZO9R_06745"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR0912797.1"
                     /translation="MENKTREAIRFEGSVQGVGFRYRMSQLARHYDVTGWVRNEYDGS
                     VSAELQGRREAIDEIIARLREDSYIYLDRVSRQKLELNEEERAFSVRY"
     gene            1420..2493
                     /locus_tag="ACZO9R_06750"
     CDS             1420..2493
                     /locus_tag="ACZO9R_06750"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR0912798.1"
                     /translation="MKRIGMLTSGGDCQALNAAMRGVVKGLSENVDELEVYGFLNGYK
                     GLIYGDYRLLTSADFSGILTKGGTILGSSRQPFKQMRVPDANGLDKVEAMKATYHKLN
                     LDCLVILGGNGTQKTANLLREEGLNIIHLPKTIDNDIYGTDVTFGFQSAINIATDCID
                     CIHTTAASHNRVFIVEVMGHKVGWLTLYAGVAGGADIILLPEIPYDIEKVIAAINKRT
                     KAGKGFTILAVAEGAISKEDAALSKKEYKEKVAKRKYPSVSYEIADRIFKETGVEVRV
                     TVPGHTQRGGSPCPYDRVLCTRLGSAAAKAIMDENYGNMVAMVNGKIKYVPLGDVAGK
                     LKTVDPNDQMIKEAKRIGISFGD"
     gene            2508..>2887
                     /locus_tag="ACZO9R_06755"
     CDS             2508..>2887
                     /locus_tag="ACZO9R_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR0912799.1"
                     /translation="MSYQALYRKFRPQEFDDVKGQDHIVTTLKNQIKADRIGHAYLFC
                     GTRGTGKTTIAKIFAKAVNCEHPVDGSPCGECPTCRAIAAGNSMNVIEIDAASNNGVD
                     NIRQIREEVEYRPTEGKYKVYIID"
BASE COUNT          792 a          607 c          869 g          619 t
ORIGIN      
        1 atgcagagag gtatgaggaa cagtatcttg cattgattga cgaacttcac aatctgccgg
       61 gacatccgta tctgtatctg atggcaccgc cggaagcatt tcctgcggag gagggagaga
      121 tcctatacgg gattaacaat gatgtgatcg gaggagagat ccggaacctt gtgccggaca
      181 tcgcggagca atcgggctgc ggaacgctgg atctctacgc gctgacgcag gatcacccgg
      241 aatatttcgt ggatggaatt cacccgaccg aggaaggata tgcgctgatt gcgcagatgg
      301 ttgcggatca gatccgggca gaccggaatg cgggcgtgtg gtgacgcggt taaaaaaggg
      361 cttcaaaaat tacaaaaaat atgatataat gaatccattc atctgaaacg gagtatggcg
      421 tagtttggta gcgcgcacgg ctgggggccg tgaggtcgca ggttcaaatc ctgttactcc
      481 gattccttgg caggggcacg gcaggcggag cgatccgtct ggcggcttaa gaaagagaga
      541 gaccgtatat tgcggtgtct cttttttatg taataggaaa cttcgttcct aattacacaa
      601 aaacgttccg cggggatgtg cagctcgcgg tgaggtacat aatgctttgc ataccgctcg
      661 ggcgcaagtg tgcggcaagc gcacactttg tttttaggaa aaaagaaaga catagaaaaa
      721 cccggcagaa tcaatttatg attctgccgg gttttgtgac agcggagacg gcgggattcg
      781 aacccgcgtg ccccggaggg ctaacgcatt tcgagtgcgc cccgttatga ccgcttcgat
      841 acgtctccga atgaatgtct cacagaggat tctactgtga gacactcgat tagtatatca
      901 tatctggttg attttgcaag atgctttcgc gaaaagtctt gaaataagtt ttttcgggag
      961 atactatttt tatagcataa caggagggta aggtatggaa aataagacac gggaagcaat
     1021 ccggtttgag ggaagcgtgc agggcgttgg attccgctac cggatgtcac agcttgcaag
     1081 acactacgat gtgacgggat gggtcagaaa tgagtacgat ggaagcgtct cggcagaact
     1141 gcaggggaga cgggaggcga tcgacgagat cattgcgcgc cttagggagg attcctatat
     1201 ttatctggac agagtgagcc ggcagaaact cgaattaaac gaagaggagc gggcattttc
     1261 cgtgcgctat taggcgctgc ggcgcgctgg tgctgtgaaa cacagttgca aaatggcgtc
     1321 gaagagggta atattctata tagaaaaact gcggtccgga tgcggaccca cagaccatga
     1381 acggatggta cgaaaagacg gaaacaggag gagaagcaga tgaagagaat cggtatgttg
     1441 acgagtggcg gagactgcca ggcattgaat gctgccatgc gcggtgttgt taagggactc
     1501 agcgagaacg tggatgaatt ggaagtatat ggttttttga atggctataa aggtctgatt
     1561 tacggggact accggctgct gacatccgcg gatttctccg gtattctgac gaaaggcggc
     1621 acgattctcg gatcatcgcg acagccgttt aagcagatgc gtgtgccgga tgcaaacgga
     1681 ctggacaagg tggaggcgat gaaggcgacc taccacaagc tcaacctgga ctgtctggtg
     1741 atcctcggag gaaacggaac acagaagacg gcgaacctgc ttcgggaaga ggggttaaat
     1801 attatccatc tgccgaagac gatcgacaac gatatttacg ggacggacgt cacattcgga
     1861 tttcagagtg ccatcaatat cgccacagat tgcattgact gtatccacac gacggcagcg
     1921 tcccacaacc gcgtctttat tgttgaggtc atggggcata aagtcggctg gctgacgctt
     1981 tatgctggcg ttgcgggggg cgcggatatt attctgcttc cggaaatacc gtacgacatc
     2041 gagaaagtga ttgccgcgat caacaagcgc acgaaggcag gaaaaggatt taccattctt
     2101 gcggttgccg agggagccat ctctaaggag gatgcggcgc tctccaaaaa agagtacaag
     2161 gaaaaagtgg caaagcggaa atatccgtct gtttcctacg agattgcaga ccgtatattc
     2221 aaggagaccg gtgtcgaggt gcgtgtgacc gtgccgggac acacacagcg cggtggaagc
     2281 ccatgtccgt atgaccgtgt gctctgcacg agactcggtt cggcagcggc aaaagcgatc
     2341 atggacgaga actacggcaa catggttgcc atggtcaatg gcaagatcaa atatgtaccg
     2401 ctcggagacg tggcgggcaa attaaaaacg gtcgatccga acgaccagat gataaaagaa
     2461 gcaaagcgga ttggaatcag ctttggagat taaggtgagg gaaaagcatg tcatatcagg
     2521 cattatatcg taagttccgt ccgcaggagt ttgacgatgt aaaaggacag gatcatattg
     2581 taacaacgtt aaagaatcag attaaagcag acagaatcgg acatgcttat ctattttgcg
     2641 gaaccagagg aacgggaaag acgacgattg ccaagatttt tgcgaaggcg gtcaactgtg
     2701 agcatccggt ggacgggagt ccgtgcgggg aatgtccgac atgcagggcg attgcggcag
     2761 gcaattccat gaacgtgatt gaaatcgatg cggcttccaa caacggcgtc gacaacatcc
     2821 gccagatccg cgaggaggtg gagtaccgtc cgaccgaagg aaaatacaaa gtctacatta
     2881 tcgatga
//
LOCUS       DBMDQJ010000290         2884 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12370_length_2884_cov_1.755300, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000290 DBMDQJ010000000
VERSION     DBMDQJ010000290.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12370_length_2884_cov_1.755300"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            83..967
                     /locus_tag="ACZO9R_06760"
     CDS             83..967
                     /locus_tag="ACZO9R_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0912800.1"
                     /translation="MTLDYYRIFYYVAQYQSFSKAAEILGNNQPNLSRCMNLLESELG
                     CKLLVRTNRGVSLTPEGQRLFEHVAIANEQLMLGEQEILKDKSLESGLVTIGASETAL
                     RLFLLNKLELFHHNYPHVRLQISNHSTPQAIRALSHGTVDFSVVTTPIDIKKPLHRTS
                     LLSFREILIGGSSYAAIASRMQSLHDLSEVPFISLGAGTGTRELYSQYFLSHHLVFSP
                     DMEASTTDQILPMVAHNLGIGFYPEALASEPIARGEVLPIRLVEPIPEREICLIRDTS
                     RPESIAARKLMEYLQRDT"
     gene            complement(1133..2092)
                     /locus_tag="ACZO9R_06765"
     CDS             complement(1133..2092)
                     /locus_tag="ACZO9R_06765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HIR0912801.1"
                     /translation="MTKHYDVTALGEVLIDFTENGSSAQGNPMYEANPGGAPCNVLEM
                     LQKFGHKTAFLGKVGEDIFGRRLEIVLKEAGIDTAGLCMDPTVRTTLTFVEKKADGDR
                     DFSFYRNPGADMMLRTEELQEQILKDTRIFHFGTLSMTDEPVRSATRHAIETAKVNGA
                     LLSFDPNIREPLWQSMEEAKRQTAYGMEVCDVLKISDNEIRWFTGEEDFDRGVDMLWR
                     EYHIPLILLSMGSGGSWAYCGSRRAEQPAFLQESTIETTGAGDTFGACCLHYILEHGM
                     AGLGTTELEEMLRVANAAASIVTTRRGALRVMPEPEEIQAFLV"
     gene            complement(2076..>2884)
                     /locus_tag="ACZO9R_06770"
     CDS             complement(2076..>2884)
                     /locus_tag="ACZO9R_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0912802.1"
                     /translation="RHPYFAELISNLESQAYRHQHKILLFNSQEKNEKEYEYLEMCSS
                     NRVAGIILCSGTVGVNEFQGLNVPLITIERFLENGTGAVECDNIQGGRLAAEHLIAQG
                     CRHLIHISGVHETAMPADERAAGFCEVCVQKNVQYQVVGTHAYEYNHLEYHEVLEQLL
                     LQHPDTDGIFASSDLIAAQLLQVCAKLGRKVPEQLKIVGFDDVNIASLTTPPITTIHQ
                     PIKEMAATAVELLVRAGEGQVVPSRTTLPVSLVVRGTTERKTEGEDDKAL"
BASE COUNT          611 a          883 c          669 g          721 t
ORIGIN      
        1 gaaacagata tgtaaaaagt atattttata tattggatat atcaatatca tatatcaagg
       61 agtgcaaaag gaggcacgga tcatgacgct ggattactac cgcatttttt actatgttgc
      121 acaatatcaa agcttttcca aagctgctga gattctgggg aacaaccagc cgaatctctc
      181 ccgctgcatg aatctgctcg agagcgagct cggctgcaaa ctgcttgtgc gcaccaaccg
      241 cggtgtcagc ctgacgccgg aggggcagcg cctctttgag catgtcgcca tcgccaacga
      301 acagctgatg ctcggggagc aggagatatt aaaagataag agtcttgaga gcggtcttgt
      361 gaccatcggc gcgagcgaga cggctctgcg cctgtttctt ttgaacaaac tggagctttt
      421 ccaccacaat tatccacatg tgcgcctgca gatttccaat cattccacac cgcaggccat
      481 ccgtgccctg tcgcacggca ccgtcgattt ttccgtggtg acgaccccga tcgacatcaa
      541 aaaacctctt cacaggacat cgcttttatc ctttcgtgaa atcctgatcg gaggttcttc
      601 ctatgccgct attgcctccc gcatgcagag tctgcacgac ctgtcggagg tcccttttat
      661 ctctctcgga gccggaaccg gcacgagaga attgtattcc cagtattttc taagtcatca
      721 tcttgtattt tcaccggaca tggaagcctc cacgaccgat cagattctgc ccatggtcgc
      781 gcacaatctc ggcatcggct tctacccgga agcgctcgcc tccgaaccga ttgcgcgcgg
      841 tgaagtgctt cccatccgtc tggtcgaacc gatcccggaa cgtgagatct gtctcatccg
      901 ggatacaagc cgcccggaga gcatcgctgc caggaagctg atggaatatc tgcagaggga
      961 tacctgaaac ggcgtcgctc aaatgctttt ttctggaaca aagtgtgcgc ttgccgcaca
     1021 cttgcgcctg ccttggcagg ctgcctgatg cggtatgcaa agcattattt acctcaccgc
     1081 gaggtgcgca tccccgcgga gcttttttgt gtaataggaa tgaagttccc tattacacaa
     1141 gaaatgcctg tatctcctcc ggctccggca tcacgcgcag cgctccgcgt cgtgtcgtga
     1201 cgattgatgc cgccgcattt gcaacacgca gcatctcttc cagttccgtc gtgccaagtc
     1261 ctgccatgcc gtgttccaga atatagtgca ggcagcatgc gccgaacgta tctcccgctc
     1321 cggtcgtctc gatcgtgctt tcctgtaaaa atgcaggttg ttctgctctg cggctgccgc
     1381 agtatgccca gcttcccccg cttcccatcg acaacagaat cagaggaatg tggtattccc
     1441 gccaaagcat gtccactccg cggtcaaaat cctcctcgcc ggtgaaccac cggatctcat
     1501 tgtccgatat tttcagcaca tcgcacactt ccatcccgta agcggtctgt ctcttcgcct
     1561 cttccataga ctgccagagc ggttctctta tgttcggatc aaaggagagc agcgccccgt
     1621 ttacctttgc ggtctcaatg gcatgtcttg tggcgcttcg caccggctca tccgtcatcg
     1681 agagcgtgcc aaaatgaaat atgcgggtgt cctttaagat ctgctcctgc agttcctctg
     1741 tacgcagcat catatccgcg cccggattcc ggtaaaaaga aaaatcacgg tctccgtccg
     1801 cttttttctc cacaaacgtc agcgtcgtgc gcaccgtcgg atccatgcag agtcccgcag
     1861 tatcaatgcc cgcctccttc agcacgattt caagcctccg cccgaagata tcctctccga
     1921 ccttgcccaa aaacgcggtc ttatgtccaa acttctgcag catttccagc acattgcagg
     1981 gtgctccgcc cggattcgcc tcatacatgg ggttcccctg tgcactgctg ccgttttccg
     2041 taaaatcaat cagcacttcc cccagtgccg tcacatcata atgctttgtc atcttccccc
     2101 tccgtcttcc tttctgtcgt tcctctgact accagagaca ccgggagtgt ggtacggctc
     2161 gggaccacct gtccctcccc tgcgcgcaca agcagttcca ccgcggtcgc cgccatctct
     2221 ttgatcggct gatgtatggt ggtaatcggc ggcgtggtca aagatgcaat atttacatca
     2281 tcaaatccca cgatcttcag ctgctctggc acttttctgc caagcttcgc gcatacctgc
     2341 agcagctgcg ccgcaatcag gtcactgctg gcaaagattc cgtccgtgtc cggatgctgc
     2401 aaaagcagct gttccagcac ttcgtggtat tccagatgat tgtactcata ggcatgggtt
     2461 cccaccacct gatactgcac gtttttctgc acacacactt cacagaatcc cgccgcacgc
     2521 tcatccgccg gcatggccgt ctcatggacg ccactgatat ggatcagatg tctgcatccc
     2581 tgggcgatca ggtgttccgc cgccagacgt cctccctgga tgttgtcgca ctccactgcc
     2641 cccgttccgt tctccagaaa tcgctcgatc gtaattaacg gtacattgag tccctgaaat
     2701 tcgttcaccc cgaccgtacc gctgcagaga atgatccctg ccacccggtt gctggaacac
     2761 atttccagat actcatactc cttctcattc ttctcctgcg aattaaagag cagaatttta
     2821 tgctgatggc ggtaggcctg actttccaga ttgctgatca gctccgcaaa atagggatgt
     2881 ctga
//
LOCUS       DBMDQJ010000291         2882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12381_length_2882_cov_1.740022, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000291 DBMDQJ010000000
VERSION     DBMDQJ010000291.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12381_length_2882_cov_1.740022"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="ACZO9R_06775"
     CDS             complement(<1..551)
                     /locus_tag="ACZO9R_06775"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR0912803.1"
                     /translation="MIMTIDVGNTNITVGVFRGDEVVSNFRITTKMPRTSDEYGMLLS
                     NLLEQNSISHDDIEDAIIASVVPNVMHSLEGAIIKYFSIRPIIVEPGTKTGIRVVTEN
                     PRQIGADRIVDAAAAYELYGGPVLVLDFGTATTYDLVDKDGAFVSGVTAPGIRISAKA
                     LWEDAAKLPEIEIRKPASILAKET"
     gene            complement(564..881)
                     /locus_tag="ACZO9R_06780"
     CDS             complement(564..881)
                     /locus_tag="ACZO9R_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6145 family protein"
                     /protein_id="HIR0912804.1"
                     /translation="MEEVVLCGASAYNKKFYLNEDFKGLPEQIKNELKIMCVLFTEDV
                     GGIFQLVFSETGELEFRTSCDENDLLYDEIGCGLKIRQLQQTKQELLEALQMYYKVLY
                     VGI"
     gene            complement(934..1911)
                     /locus_tag="ACZO9R_06785"
     CDS             complement(934..1911)
                     /locus_tag="ACZO9R_06785"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR0912805.1"
                     /translation="MKAELLSVLRDADDYVSGQDLCEKFGVSRTAVWKGINQLKEAGY
                     EIEAVPNKGYKLLSVPDTLSEEELKSIRKTAWVGQEIFYYPVIDSTNTRAKQLAEEDY
                     PTGTLVVAGQQDAGRGRRGRSFESPQGAGIFMTLMLKPELSPVNAPMLTLVAALAVSA
                     AIVKTTGRPAGIKWPNDIVMNGKKVCGILTEMSAQMDYVNHIVIGIGINVHNESFPEE
                     IAPVATSLYMETGEHFNRAALIEEVWEQFEHYYAIFMETEDLSGLVKEYDTHLINRNQ
                     IVKVLDPKEPFEGKAMGITPRGELIVDTWESRRLVSAGEVSVRGVYGYV"
     gene            complement(1923..>2882)
                     /gene="argF"
                     /locus_tag="ACZO9R_06790"
     CDS             complement(1923..>2882)
                     /gene="argF"
                     /locus_tag="ACZO9R_06790"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - L-arginine biosynthetic process
                     via ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR0912806.1"
                     /translation="LMDYTPEEILYLIDLAADLKSKKKQGILHDSLIGKNIALIFEKT
                     STRTRCSFEVAAHDLGMHVTYLDPSGSQIGKKESIPDTARVLGRMFDGIEYRGYGQDI
                     VEELAKYAGVPVWNGLTNEFHPTQMLADMLTVREHLEHLKGVKFVYMGDARYNMGNSL
                     MVTCAKLGMDFVACTSKKYFPNEELVDYCKKVAAGTGASITLTEDVAEGTKDADVIYT
                     DVWVSMGEPDEVWAERLRDLMPYQVNKAAMANAKPTAIFMHCLPAFHDLKTKIGKEVY
                     EKFGYSELEVTDEVFESEQSVVFDEAENRMHTIKAVMLATLGE"
BASE COUNT          633 a          890 c          604 g          755 t
ORIGIN      
        1 gtctccttcg cgaggatgct cgccggcttg cggatctcaa tctccggaag cttcgccgca
       61 tcttcccaca atgcttttgc ggaaatgcgg attcctggag ctgtaacgcc ggatacaaac
      121 gcgccgtcct tgtccaccag atcgtacgtg gttgccgtgc caaaatcaag gacaagcacc
      181 ggtccgccat acagctcata cgctgccgct gcatccacaa tacggtccgc accgatctgg
      241 cgcgggttct cagtcacgac acggataccg gtcttggttc ccggctccac aataatcggc
      301 cggatgctaa aatacttgat aatcgccccc tcaagggaat gcatcacatt cggaacaacc
      361 gatgcaataa tcgcatcctc aatatcgtca tgtgaaatcg aattctgctc cagcaggttg
      421 ctcaacagca tcccgtattc atcggatgta cggggcattt tcgtcgtgat acgaaaattc
      481 gataccacct cgtctccccg gaatactccc acggtaatat ttgtgtttcc aacatcaatc
      541 gtcattatca tcgtccgtac cccctatatt cccacatata aaaccttata atacatctgc
      601 agcgcttcca gcagttcctg ttttgtctgc tgtaactgac ggatcttaag tccgcagccg
      661 atctcgtcgt acagcagatc attttcatcg caggaggtgc ggaattccag ttcgcccgtt
      721 tcgctgaaca ccagctgaaa aataccgccc acatcctccg tgaaaagcac gcacatgatc
      781 ttcagttcat tcttgatctg ctccggaaga cctttaaagt cctcgttcag ataaaatttt
      841 ttattgtatg cgctcgcacc gcacagtacc acttcttcca ttcagacatt cctttccaaa
      901 ccgtttccac agacactgtt tgcacagtat cccttacaca tatccgtaga caccgcgcac
      961 agagacttct cctgcagata caagtcttct gctctcccag gtatccacga tcagctcgcc
     1021 ccgcggcgtg atgcccatcg cttttccctc aaacggttcc ttcggatcaa gcaccttcac
     1081 gatctggttc cggttgatca gatgcgtatc gtactccttc acaagaccgc tcagatcctc
     1141 ggtctccatg aaaattgcat aataatgttc aaactgttcc catacttcct caatcagagc
     1201 cgcgcggtta aaatgttctc cggtctccat gtagagcgaa gttgccaccg gggcaatctc
     1261 ctccgggaac gattcattgt gaacgttgat gccaatgccg atcacaatgt gattgacata
     1321 atccatctgt gcgctcatct ccgtcagaat accgcaaact tttttcccgt tcatgacaat
     1381 gtcattcggc catttgatgc ccgccggtct gcctgtcgtc ttcacgatcg ccgcagacac
     1441 cgcaagcgcc gccaccagcg tcagcatcgg cgcattcacg ggcgacaact ccggctttaa
     1501 catcagcgtc ataaaaatgc cggctccctg cggggactca aagcttcttc ctcttctgcc
     1561 tctgcctgca tcctgctgtc cggcgaccac aagcgttccg gtagggtaat cttcctccgc
     1621 cagctgcttc gccctggtat tggtggagtc gatgaccgga tagtaaaaaa tctcctgtcc
     1681 gacccacgcg gtcttgcgga tactttttaa ttcttcctcg ctcagtgtgt ccggcaccga
     1741 caacagctta tatcccttgt tcggcaccgc ctcgatctca tacccggctt ccttgagctg
     1801 gttgatgccc ttccagaccg ccgtccggga tacgccgaac ttttcacaca gatcctgccc
     1861 ggacacgtaa tcgtcggcat ctcttaaaac ggaaagtagt tctgctttca tgatgttccc
     1921 tcttattctc ccagtgtagc aagcatgact gccttaatcg tgtgcatgcg gttctctgcc
     1981 tcgtcaaata caaccgactg ctcactctca aatacttcgt cggtcacctc aagctcgctg
     2041 taaccgaatt tttcatagac ttccttaccg atcttggtct ttaagtcatg aaatgccggc
     2101 aggcagtgca tgaagatcgc cgttggcttg gcatttgcca tggctgcctt gttgacctga
     2161 tacggcatca gatcccgcag gcgctctgcc cagacttcgt ccggctcacc catcgatacc
     2221 cagacatccg tgtaaatgac gtccgcgtcc ttcgtgcctt ctgccacatc ctcagtcagc
     2281 gtgatcgacg ctcctgtccc ggccgccact tttttgcagt aatccaccag ctcctcgttc
     2341 ggaaaatatt tcttagaagt acatgcgaca aagtccatac cgagctttgc gcaggtcacc
     2401 atcagggaat tgcccatatt gtagcgggcg tcgcccatgt atacaaactt cacacccttt
     2461 aagtgttcca ggtgttcccg gaccgtcagc atatctgcaa gcatctgcgt cggatggaac
     2521 tcattcgtca gaccgttcca cactggaacc cctgcatatt ttgccagttc ctccacaatg
     2581 tcctgtccgt atccgcggta ctcgataccg tcaaacatgc gtcccagcac gcgcgcggta
     2641 tccggaatgc tctctttttt gccgatctgc gaaccggatg gatcaaggta agtcacatgc
     2701 attccgagat catgtgctgc cacctcaaaa gaacaccgtg tgcgggtgct tgtcttctcg
     2761 aaaatcagcg cgatattttt tccgatcaga ctgtcatgca gaattccctg cttcttcttg
     2821 ctcttaagat ccgctgccag atcaatcagg tagagaatct cttccggtgt gtaatccatc
     2881 ag
//
LOCUS       DBMDQJ010000292         2881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12389_length_2881_cov_1.302158, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000292 DBMDQJ010000000
VERSION     DBMDQJ010000292.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12389_length_2881_cov_1.302158"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="ACZO9R_06795"
     CDS             <1..199
                     /locus_tag="ACZO9R_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR0912807.1"
                     /translation="IATAYAKFPQETHAFLLENELDDFTYNKAIQKMLESYRVSAEDK
                     EMLRGMKRKATNRKNAAKPGR"
     gene            501..1718
                     /locus_tag="ACZO9R_06800"
     CDS             501..1718
                     /locus_tag="ACZO9R_06800"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014120145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR0912808.1"
                     /translation="MARFTLPRDLYHGKGALEALKDFEGKRAMICVGGGSMKRFGFLD
                     RAKQYLEEAGMEVCLFEGIEPDPSVETVMKGAKAMEEFKPDWIVAMGGGSPIDAAKAM
                     WIKYEYPDITFEDMCKVFGIPKLRKKAHFCAISSTSGTATEVTAFSIITDYEKGIKYP
                     IADFEITPDVAIVDPELAETMPQKLVAHTGMDAMTHAIEAYVSTANCDFTDPLALHAI
                     KMIQRDLVGSYNGDMEKRDSMHNAQCLAGMAFSNALLGIVHSMAHKTGAAFADYGAHI
                     IHGAANAMYLPKVIAFNAKDETAKKRYGEIADFMGLGGDTLDEKVALLITYLRGMNDD
                     LKIPHCIKNYGADSYPTEQGFVPEEVFLERLPEIAANAILDACTGSNPRQPSQEEMEK
                     LLKCCYYDTEVDF"
     gene            2204..>2881
                     /locus_tag="ACZO9R_06805"
     CDS             2204..>2881
                     /locus_tag="ACZO9R_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HIR0912809.1"
                     /translation="MEIQVGKQSLAFAEAPYIISSASIVGKKEGEGPLGNRFDVVGED
                     DKFGQNTWEEAESTLQKEAFTLAVGKAGLKTEDIRYLFSGDLLGQNIATSFGLMDYQV
                     PLFGLYGACSTCGEALSLGAMCVAAGYADYVVAMTSSHFASAEKQFRFPLEYANQRPK
                     SATWTVTGSGAYVLGKKRSKARITGITTGKIVDYGIKDSMNMGAAMAPAASDVICRHL
                     SDFGRNVT"
BASE COUNT          821 a          627 c          813 g          620 t
ORIGIN      
        1 aattgcaact gcctatgcaa aatttccgca ggagacccat gcatttctgc tggaaaatga
       61 actggatgat tttacctata ataaggcaat tcagaaaatg ctggagtcat accgtgtaag
      121 tgcggaggac aaagaaatgc tgcgtggtat gaagcggaaa gcgactaaca gaaagaatgc
      181 cgccaagccc gggcggtaag aatcctaaaa accctaaaaa acctaaaaaa ctaaaaaacc
      241 gagtcatctg ctttagatgt gaagcccctg gaatccaccc caacctgcca cgccggaaca
      301 tgggaacggc atatcagaac acgatgcgaa aatctcttgt gaaaatgtgc agaatagaaa
      361 cggaaagaat tgccacgccg gttgtccata tgttatactt aacatatgca gaaacgacgc
      421 cggatttggg gtatgatagt aacggcgggc aaaggctgta aaaaatggtt gccacaggaa
      481 gtggcggatt ggaggagcat atggcaaggt ttacgttacc gcgcgatctg tatcacggaa
      541 agggagcgct ggaggcttta aaggattttg agggaaagcg cgcgatgatc tgtgtcggcg
      601 gaggttccat gaagcgtttt ggatttctgg atcgcgcaaa gcagtatctg gaagaagcag
      661 gaatggaagt ctgtctgttt gagggaattg agcctgatcc atcggtggag acggtgatga
      721 agggcgccaa ggcaatggaa gaattcaagc cggactggat cgtggcgatg ggcggcggtt
      781 ctccgattga tgcggcaaag gcaatgtgga tcaagtatga atatccggac attacgtttg
      841 aagatatgtg caaggtattc ggaattccga agctccgcaa aaaagcacat ttctgcgcga
      901 tctcttccac atccggaacg gcgacggaag tgacggcatt ttccatcatt acggattatg
      961 aaaaaggaat caaatatccg attgcagatt ttgagattac gccagatgtg gcgatcgtgg
     1021 atcctgagct cgcagagacg atgccgcaga agctggtggc gcataccggt atggatgcga
     1081 tgacgcatgc catcgaggcg tatgtttcca cggcgaactg tgattttacc gatccgcttg
     1141 ccctgcatgc gatcaagatg atccagagag atcttgtggg ctcttataac ggagacatgg
     1201 aaaaacgtga ttccatgcac aatgcacagt gtctggcggg tatggcattt tccaatgcac
     1261 tgcttggaat cgttcattcg atggctcaca agacgggtgc tgcatttgca gattacggcg
     1321 cacatatcat tcacggtgca gcaaacgcca tgtatctgcc gaaggtcatc gcattcaacg
     1381 ccaaggatga gactgcgaaa aagcgctacg gcgagatcgc agattttatg ggactgggcg
     1441 gagatacgct ggatgaaaaa gtggcgctcc tcattacata tctgcgcggc atgaatgatg
     1501 atcttaagat tccgcactgt atcaagaatt acggcgcgga cagctatccg accgagcagg
     1561 gatttgtgcc ggaggaagta ttcttagaga gactgccgga gattgcagca aatgcaattc
     1621 tggatgcctg cacgggatcg aatccgcgtc agccgagcca ggaggagatg gaaaaactgt
     1681 tgaagtgctg ctactacgat acagaggtag atttctagaa gagcatattt ttaagacagc
     1741 tgaaacatca tccataatgt gataaaatac agcgcgatcg atgacaataa cagtacggtc
     1801 aacgatcaca gaggtcagtg ataacacaac caatggtcat acgataaaac agagggtgca
     1861 catgcgtgcc ggaaatccgg cactcgcatg caccctctgt tttgtctgcc ggcgaaattt
     1921 cgtttttgac tagcagtccg atcatttcaa gatcatgcga aacatttctg aacagtcaaa
     1981 ccaatttcac gcccagccgc cccaatttcc ggacagttaa actatcatcc gggcaacgaa
     2041 acatttttcg caataacaaa ccatattcac aacagaaaaa gtcgaaccac tactggaaag
     2101 tttgtgaaca gaagaaaaca aaaatcaaaa tggtatttta ccggtataat acacgtttgt
     2161 gcgccgcata ctaaggaaaa gtcttgcgaa aggaagcagt gatatggaga tacaggtcgg
     2221 gaagcagagc ctcgcgtttg cggaggctcc ttacatcata agcagtgcga gtattgtggg
     2281 gaaaaaagag ggcgaaggac cgctcgggaa ccgcttcgac gtggtgggcg aggacgacaa
     2341 gttcggacag aacacctggg aggaggcgga gagtaccctg cagaaggaag catttacgct
     2401 ggcggtcgga aaggcaggct tgaaaaccga ggacatccgt tatctgtttt cgggggatct
     2461 gctcgggcag aacattgcga catccttcgg gctcatggac tatcaggtgc cgctgttcgg
     2521 actttatgga gcgtgcagta cctgtggcga agcgctctca ttgggcgcga tgtgcgtggc
     2581 ggcggggtat gcggattatg ttgtggcgat gacgtcgagc cattttgcca gcgcggaaaa
     2641 gcagttccgt tttccactgg aatatgccaa ccagcgcccg aaatcggcga cgtggacggt
     2701 cactggaagt ggcgcctatg tgctcgggaa aaaaagaagc aaggcgcgca tcacggggat
     2761 cacgaccggc aagatcgtcg attacggcat caaggattcg atgaatatgg gagcggccat
     2821 ggcacctgcc gccagtgacg tgatctgccg ccatctgtcg gatttcggaa gaaacgtgac
     2881 g
//
LOCUS       DBMDQJ010000293         2879 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12395_length_2879_cov_1.463283, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000293 DBMDQJ010000000
VERSION     DBMDQJ010000293.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2879)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12395_length_2879_cov_1.463283"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="ACZO9R_06810"
     CDS             complement(<1..418)
                     /locus_tag="ACZO9R_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0912810.1"
                     /translation="MNIINAENLTKSYTERKLLDKASFYLQEGEKVGVIGINGTGKST
                     LLKIIAGLEEPDEGQVTCANHIVVRYLPQNPVFDPEMTVLDSVLTQCVLNFVGSGRTE
                     RTDADAHAQEQKWSLESDAKSMMTRLGITDFTQKTGE"
     gene            complement(463..2445)
                     /locus_tag="ACZO9R_06815"
     CDS             complement(463..2445)
                     /locus_tag="ACZO9R_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR0912811.1"
                     /translation="MQYNYDFEIASLLIMTIILLHFVFVRQFPGEKTRVFGWLLFVCE
                     AECLMNILSCVGLANAALVPQRLNEVLAFAFFVLEGASSYLIYRYFMAACALRGRERT
                     VFAAAGMIPFAVFLVLVAVTPVTGFFYYFRDGSYYRGVGADYGYAYIVIFFLLGLLIV
                     VRQHKYTTLRTKVIVCAYTAVAAAMIGLQYWHREMLCTSVSNTVVLIMLYLQIQNPVV
                     FLDTTTGIGNGTAFESQLEDRLRRKGEGYVLTIHLSKFYHIHTILGTENSNELLREIG
                     AYLYNLCGKFHVFHTAGDAFTVFADTKEQCERLKCEIQKRFESDWVVQENRIALDMEM
                     VVQHYPTDFKAMAEYYGMREFLLENAGKSGAQVIVEADADMIAQYRRRRKVEIAVARA
                     IREKGFLVYYQPVYSLKKRQIVSLEALVRLKDPELGFISPEEFIPLAERDGNIIHIGE
                     QVLEQCCRFLSKHVLSNGSLGIRTIHVNISMVQCLRQNLTETIRPVLESYHIPPSMLT
                     LEVTERTAIGAPERMLWHMHELGKMGVSFALDDYGSGNANCSYLIRFPFQEIKIDKEI
                     VWASFHDKAARIVLENEIHTIKQLGIPLIIEGIEEREQSEAMEQLGVECIQGYYYGRP
                     LPEQECLRYIRTFQEKAQNDRQQAAVPGEKQREAGR"
     gene            complement(2435..>2879)
                     /locus_tag="ACZO9R_06820"
     CDS             complement(2435..>2879)
                     /locus_tag="ACZO9R_06820"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HIR0912812.1"
                     /translation="NMVNYINDIAGIRIVCSFTSDIYRLAEMIGKQNDLTVISVKDYI
                     RHPKESGYKSYHMLVTVPIFLSDRIIDTKVEIQIRTMAMDFWASLEHKIYYKFEGNAP
                     DYISRDLRECSGIVSMLDAKMLQLNEAILEAKAAQEEQEGDTGAV"
BASE COUNT          683 a          877 c          598 g          721 t
ORIGIN      
        1 gttctccggt tttctgtgta aaatcggtaa tgccgaggcg cgtcatcatg ctttttgcat
       61 cgctctcaag gctccatttc tgttcctgtg catgcgcgtc ggcatcggtg cgctctgtcc
      121 tcccgctgcc gacaaaattt aagacacact gtgtcagcac actgtcaagt accgtcatct
      181 ccggatcgaa caccggattc tgcggcaggt atcttaccac gatatggttg gcgcaggtca
      241 cctggccttc atccggttcc tccagccccg caatgatttt taacagggtg gactttcccg
      301 tcccgttgat accgattacc ccgacttttt ctccctcctg cagataaaag gaagctttgt
      361 ccaaaagctt cctctctgtg tatgatttcg ttaaattctc cgcgttaata atattcatgt
      421 ctcttctctt tctttcctat ttctgtatga ctcaaatggc tcttaccgtc ctgcttccct
      481 ctgcttttct cctggaacag ccgcctgctg cctgtcgttt tgcgctttct cctgaaacgt
      541 gcggatatac cgcaggcact cctgttccgg cagaggtctg ccgtaatagt atccctgaat
      601 acattccaca ccaagctgtt ccatcgcttc gctctgctcc cgctcttcaa ttccctcgat
      661 aatcagcgga atccccagct gtttgatggt gtgaatctca ttttcaagca cgatgcgcgc
      721 cgccttgtcg tggaacgacg cccacacgat ctccttgtca atcttgatct cctggaatgg
      781 aaaacggatc agataggaac agttcgcatt tccgcttcca taatcgtcaa gtgcaaacga
      841 cactcccatt tttcccagct catgcatatg ccacagcatc cgctccgggg cgccaatcgc
      901 cgtgcgctcc gtcacctcca gcgtcagcat ggacggcgga atatggtaac tctcaagcac
      961 cggtcggatg gtctcagtca gattctggcg cagacactgc accatcgaaa tgttcacatg
     1021 gatcgtacgg attccaagcg agccgttcga gagcacatgc tttgaaagaa accggcagca
     1081 ctgctccagc acctgttccc cgatatggat gatattgcca tcgcgctccg caagcggaat
     1141 aaactcctct ggcgaaataa atcccaattc cggatcctta agccgcacca gcgcttccag
     1201 ggacacaatc tgccgtttct tcagggaata taccggctga tagtagacca gaaacccctt
     1261 ctcccggatc gccctcgcca cggcaatctc cacttttctg cggcggcggt actgtgcgat
     1321 catgtccgca tccgcctcca caatgacctg cgcgccggac tttcccgcat tttcaagcaa
     1381 aaactcccgc attccgtaat attccgccat cgccttaaag tccgtcggat agtgctgcac
     1441 caccatctcc atatccagcg caatgcggtt ctcctgcacc acccagtcac tttcaaaccg
     1501 cttttgtatc tcacatttta acctttcaca ctgctctttg gtgtctgcaa acaccgtaaa
     1561 tgcatctcct gccgtgtgaa acacatgaaa ttttccacac agattataca gatacgcccc
     1621 gatctcgcgc agcagttcat tgctgttctc ggtacccaga atcgtgtgga tatgataaaa
     1681 cttgctcaaa tgaatcgtga gcacatagcc ctcgcccttt ctgcggagcc ggtcctccag
     1741 ctgcgattca aatgccgtcc cgttcccgat tcctgttgtc gtgtccagaa acaccaccgg
     1801 attctgaatc tgcagataga gcattatgag cacgaccgta ttgctgacgc tggtgcaaag
     1861 catctccctg tgccagtatt gcaacccgat catggcggct gccacagccg tgtaggcgca
     1921 cacgatgacc ttcgtgcgaa gcgtggtata tttatgctgc cgcacgacaa tcagaagccc
     1981 caacaaaaag aagatcacaa tatacgcata tccgtaatcc gcgccgacgc cccgataata
     2041 actgccatcc ctaaagtaat aaaaaaatcc agtcaccggc gtcactgcca ccaacacaag
     2101 aaacacggca aacggaatca tgccggcagc cgcaaatacc gttctctccc tgccgcggag
     2161 cgcacatgcc gccataaaat accggtaaat cagatatgac gaagccccct ccagcacaaa
     2221 aaaagcaaac gccagtacct cgttcagacg ctgcggaacg agggctgcat ttgctaatcc
     2281 cacacatgat agaatgttca tcagacactc tgcttcacac acaaacagaa gccagccgaa
     2341 tacccgtgtc ttctccccgg gaaactggcg cacgaataca aagtgcagta atataattgt
     2401 cataattaag agtgatgcga tctcaaaatc ataattatac tgcacctgta tctccctcct
     2461 gctcttcctg tgccgccttt gcctccagaa ttgcctcatt gagctggagc atctttgcgt
     2521 ccagcatgga aacgattccc gaacactcgc gcagatccct gctgatgtaa tccggcgcat
     2581 ttccctcaaa cttatagtag atcttgtgct cgaggcttgc ccagaaatcc atcgccatcg
     2641 tccggatctg aatctccacc ttcgtgtcga taatgcggtc cgacaaaaag atcggtaccg
     2701 tcaccagcat gtgatagctt ttgtatccgc tctcctttgg atgccggata taatccttca
     2761 cggagattac cgtgagatca ttctgcttgc cgatcatctc ggcaaggcgg taaatatccg
     2821 acgtaaaaga acagacaatc cggattcccg caatgtcgtt gatgtaattc accatattg
//
LOCUS       DBMDQJ010000294         2871 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12433_length_2871_cov_1.503249, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000294 DBMDQJ010000000
VERSION     DBMDQJ010000294.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12433_length_2871_cov_1.503249"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="ACZO9R_06825"
     CDS             complement(<1..343)
                     /locus_tag="ACZO9R_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR0912813.1"
                     /translation="MKVIDAAALDYKTLNEVLRQPEHDYVIEGCCGQRFIGAGMSDRN
                     ITVNGISGNALGAYLNNASITVNANAQDAVGDTMNAGKILIHGSAGDAAGYAMRGGKI
                     YVRDHAGYRAGI"
     gene            complement(380..1885)
                     /locus_tag="ACZO9R_06830"
     CDS             complement(380..1885)
                     /locus_tag="ACZO9R_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013784.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR0912814.1"
                     /translation="MSIEFIYPEFEVIRNENRCITCRICQQQCANGVHSYNSETKQMV
                     CDESNCVNCQRCVSFCPTRALKIVKSDCSLRENANWSENMMKEIYKQANSGGVLLSSM
                     GNPNPLPVYWDRILINASQVTNPPIDPLREPMETRIYLGKKPEQVHRMADGSLDCELP
                     PQLELSMPVMFSAMSYGSISYNAHKSLALAAKELGILYNTGEGGLHEDFYCYGENTIV
                     QVASGRFGVHEKYLNAGAGIEIKMGQGAKPGIGGHLPGTKIVGDVSRTRMIPEGSDAI
                     SPAPHHDIYSIEDLRQLVCSLKEATEYKKPIIVKVAAVHNIAAIASGIARSGADIIAI
                     DGFRGGTGAAPTRIRDNVGIPVELALAAVDQRLRDEGIRNHVSLVAGGSIRSASDVVK
                     AVALGADACYVATAALLALGCHLCRTCQSGKCNWGIATQNPELVERLDPQTGSRRLIN
                     LMTAWKHEIKELMGGMGINSIEALRGNRLMLRGIGLNEKELEILGISHAGE"
     gene            complement(1882..>2871)
                     /locus_tag="ACZO9R_06835"
     CDS             complement(1882..>2871)
                     /locus_tag="ACZO9R_06835"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HIR0912815.1"
                     /translation="PMHDRSNGLGGGFAGYGIYPEYKDFYAFHMFFDSRAARKGCEAF
                     LKERFEIVQSEIIPTRKTPLITDEPIIWRYFVAPLKSLLTSMQLDEKEYVVRTVMKIN
                     TEMPGAYVFSSGKNMGTFKAVGYPEDIGIFYKLDEYEGYSWTAHGRYPTNTPGWWGGA
                     HPFALLDSSVVHNGEISSYDANRRFMEMFGYKCTLQTDTEVITYILDYLLRRQNLTLG
                     EAASVIAAPFWSTIAAKTDLADQKKHTYLRTLFPSLLITGPFSIVFGFNGGLMALNDR
                     LKLRSMVVGEKKDMVYIASEETAIRAMEPDAENIWAPAGGEPVIVTVKEGVLL"
BASE COUNT          694 a          714 c          684 g          779 t
ORIGIN      
        1 gaatgcccgc ccggtagccg gcatggtcgc gaacataaat tttaccgcct ctcatagcgt
       61 atccggccgc atctccggcg ctgccatgaa tcaaaatctt tcctgcgttc atcgtatcgc
      121 ctacggcgtc ctgcgcattt gcgtttacgg taatagaggc gttatttagg taggcgccga
      181 gagcattgcc ggaaattcca ttgacggtaa tattccggtc agacattccg gcgccgataa
      241 agcgctgtcc gcagcagcct tcgatcacat aatcgtgttc cggctgccga aggacttcgt
      301 ttaaagtttt ataatctagt gcagcagcat ctatcacttt catagtataa cacatctcct
      361 tgataaaatg atattgtttt tattctccgg catgggaaat gccaagtatt tcaagttctt
      421 tttcgtttag cccgattccg cggagcataa gacggttgcc ccggagagct tcgatggagt
      481 taattcccat acctcccata agttctttga tttcatgttt ccaggcagtc atcagattga
      541 taagccgacg gcttcccgtc tgcggatcaa gtctttccac aagttcagga ttctgcgtgg
      601 cgatccccca gttacacttg ccgctttgac acgtgcggca gagatgacac ccaagagcca
      661 gaagcgcggc tgttgcaaca taacaggcgt ccgctccaag ggctactgct tttaccacat
      721 cggaagcgct gcggatactg ccgcctgcta cgagcgaaac gtgatttcgg attccttcat
      781 cacgaagcct ctgatccact gccgccagag cgagctctac agggattccc acattgtcgc
      841 gtattctggt aggagcggcg ccggtgcctc cgcggaagcc gtcgatagcg ataatgtccg
      901 cgccgctgcg tgcaattccg ctggcgatgg ctgcgatatt atggacagcc gccactttta
      961 cgataatcgg ttttttgtat tccgtagcct ctttcagaga acagaccagc tgccgcagat
     1021 cttcaataga atagatatca tgatgaggcg cgggggagat ggcgtcagag ccttcaggga
     1081 tcattctggt gcgggacacg tctcctacaa ttttagttcc cggaagatgc cctccgattc
     1141 ccggttttgc gccctgtccc attttgatct caatgccggc gccggcgttt aaatattttt
     1201 catgaacgcc aaagcgtcct gacgctacct gtacaatggt gttttctcca tagcaataaa
     1261 aatcttcatg gagaccacct tctcctgtat tatataaaat cccaagctct tttgcagcga
     1321 gagccagtga tttgtgcgca ttataactga tagagccata gctcatggcg gagaacatga
     1381 ccggcataga gagttccagc tgcgggggaa gctcacagtc taaagaaccg tccgccatcc
     1441 gatgcacttg ttccggtttc tttccaagat aaatccgggt ttccataggc tctcttagag
     1501 ggtcgatggg agggttggtt acttgcgaag cattgattaa gatgcgatcc caataaacgg
     1561 gaagaggatt tgggtttccc atggaagaga gcaggactcc gccgctgttt gcctgtttat
     1621 aaatttcttt catcatattt tccgaccagt tggcattttc gcgtagagaa cagtcgctct
     1681 ttacgatttt taaagctctg gtgggacaaa aggaaacaca tctttggcag tttacacagt
     1741 tgctttcgtc acataccatc tgtttggttt cactattgta ggaatgtacg ccattggcgc
     1801 attgctgctg gcagatacgg caggtaatgc agcggttttc atttcggatc acttcaaact
     1861 cagggtagat aaattcaatg ctcataataa aacaccttcc tttactgtta cgataaccgg
     1921 ttctccgccg gcgggagccc agatattttc ggcatccggt tccatggctc ggatggctgt
     1981 ttcttcactg gcaatataaa ccatatcttt tttttctcct acgaccatgg agcggagctt
     2041 cagccggtca ttgagcgcca taagccctcc gttgaaaccg aaaacaatgg agaaaggtcc
     2101 ggtgatcaga aggctgggga acagagttcg taagtaagtg tgctttttct gatctgcaag
     2161 atctgttttt gctgcaatgg tgctccaaaa gggggcggca attacgctgg cagcttctcc
     2221 aagagtcaga ttctgcctgc ggagcagata gtctaaaata tacgtgatga cttccgtatc
     2281 cgtctgaagg gtacacttat atccaaacat ttccataaaa cggcggttgg catcatagga
     2341 agaaatttca ccgttatgta caacggaaga atccagaaga gcaaaggggt gagctccgcc
     2401 ccaccagccc ggagtattgg taggatagcg gccgtgggca gtccaggaat atccctcgta
     2461 ttcgtctaat ttatagaaaa taccgatgtc ttccgggtat cccactgctt tgaaagttcc
     2521 catgtttttg ccgctggaaa agacataagc tcccggcatc tccgtattga tcttcatgac
     2581 tgtacgtacc acatattcct tttcatccag ctgcatagac gtcagaagag acttcaaagg
     2641 agctacaaag tatctccaga tgataggctc atctgtgatc agaggcgttt ttcttgtggg
     2701 gataatttct gactgtacga tctcaaaccg ttcttttaaa aacgcctcgc atccttttct
     2761 ggcagctctg ctgtcaaaaa acatatgaaa ggcataaaag tctttatatt ccggatagat
     2821 tccataaccg gcaaatccgc cgccaagacc attggatcgg tcatgcatgg g
//
LOCUS       DBMDQJ010000295         2871 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12434_length_2871_cov_1.325632, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000295 DBMDQJ010000000
VERSION     DBMDQJ010000295.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12434_length_2871_cov_1.325632"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="ACZO9R_06840"
     CDS             complement(<1..406)
                     /locus_tag="ACZO9R_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912816.1"
                     /translation="MGELILCNQNMAAFPYYVEEAAIGVYSLEELSYYICHNVYLLRS
                     DFMNEDLCNWLEWELKLKDAADALREIVRKGGTLSEFVTCLLSQSGYCDRMQIAQIAQ
                     QIAELEHKSEYECSKIRADRYVTNGRYAAAVSA"
     gene            complement(424..1665)
                     /locus_tag="ACZO9R_06845"
     CDS             complement(424..1665)
                     /locus_tag="ACZO9R_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5716 family protein"
                     /protein_id="HIR0912817.1"
                     /translation="MEHRFYLGIDLDDQYAVISSYELNKKEPETFSAIAGSEIYQIPA
                     LITKKKGIGQWFVGEEAVRLAIAQGEEANGRLLSRAVGGEKVFVDGETYPAEELLTLY
                     LKKLIRLACGPDRRWEPERLVICMEKLSREATELFLSMAPKLGIDREKLQLLDRKECF
                     YYFAYHQVPELSMHDVYLFDYRREDIRCCRLYKEKRTTPQLISLAEEVRRMNADSRDE
                     NFLGILKDCFRGHIVSSVYLTGDGFDGDWMKQSVAFLCQGRRAFVGKNLYSKGACYAA
                     LCSEQQENWDYVYLGDNEMKVNVSLKVRNMGKAEFYTLISAGDNWYETQGMCEVILAG
                     TPEIDFWLQPPGTREAKVEKLKLLDLPERPDKATRLRITAKPVSDTKVWIQIRDLGFG
                     ELFKSSDKVWDYRMEFSEENP"
     gene            complement(1683..>2871)
                     /locus_tag="ACZO9R_06850"
     CDS             complement(1683..>2871)
                     /locus_tag="ACZO9R_06850"
                     /inference="COORDINATES: protein motif:HMM:NF039514.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5717 family protein"
                     /protein_id="HIR0912818.1"
                     /translation="VRLALALISYEKKDGRDFSYSEGELDLMKGQLAEALSDVSVRRQ
                     MAALLTENHLYEKAYQMVQIDGYDYLKTEWRVSLCSYAIADHGYEEDDFLLGFAESTF
                     RQGKYSDVMLIYLCKYFNGPTKQMAELWKAAGEFQIDTFDLEERILTQMLYTTDYTPH
                     MEEIYESYCAGGGRDLVCMAYLSYFANMYLTEDAVVPEHVFRQIRERYLTGGDLNRVC
                     RIALLKYLSENHLSERDRKIADELLDTCIRHNLYFAFFRKFDEKLLRKYQLTDKFFLE
                     YHTAPGQQIEVHYRLEAQQEYLADELTEMYDGIYVKDFILFFGEKVQYYILESQTADG
                     QIGASGCLGNGDIMEEGSGDRYAWLNEMLLACTLDDREKLAGKMKRYYGMTKVTEEAY
                     RLL"
BASE COUNT          636 a          906 c          613 g          716 t
ORIGIN      
        1 acgccgagac ggctgccgca tagcgcccgt ttgtcacata acggtcggcg cggatcttgc
       61 tgcactcgta ttcggatttg tgttccagct ccgcgatctg ctgcgcaatc tgcgcgatct
      121 gcattctgtc acaatacccg ctctgcgaca gcagacaggt cacaaactcg gacagggtgc
      181 cgccttttct cacaatctcc cgaagggcat ctgcagcatc ttttagtttc agttcccatt
      241 ccagccagtt gcagagatcc tcattcataa aatcactgcg gagcagatag acattgtggc
      301 agatataata acttaattcc tccagcgagt aaacgccgat tgccgcctcc tccacataat
      361 atggaaatgc cgccatattc tgattgcaca gaatcaattc tcccatcgtc ttctcccaca
      421 cctctacgga ttctcttccg aaaattccat ccggtaatcc cacactttat ccgaactctt
      481 aaataattcc ccgaatccaa gatcccggat ctggatccag acctttgtat ccgaaaccgg
      541 ttttgccgtg atgcgaagcc tcgttgcctt gtccgggcgt tccggcagat ccagaagctt
      601 tagcttctcc accttcgcct cccgggttcc cggcggctgc agccagaaat caatctccgg
      661 cgttcccgca aggatcactt cgcacattcc ctgcgtctcg taccagttgt cccctgcact
      721 gatcagcgta tagaactctg cttttcccat attccggact tttaagctga cattgacttt
      781 catctcattg tcacccagat atacataatc ccagttttcc tgctgttcac tgcacaaggc
      841 ggcgtagcag gcccccttgg agtacagatt cttcccgaca aacgctctcc tgccctgaca
      901 cagaaacgcc acggactgtt tcatccagtc cccgtcaaat ccgtctcccg tcaggtacac
      961 cgacgagaca atatgtccgc ggaagcagtc ctttagaatg ccaagaaaat tctcgtcccg
     1021 gctgtccgca ttcatccggc gcacttcctc cgccagcgag atcagctgcg gcgtcgtgcg
     1081 tttttcctta tataatctgc agcagcggat atcctccctg cggtaatcga acagatacac
     1141 gtcgtgcatc gaaagttccg gcacctgatg gtaggcaaaa tagtaaaaac attctttccg
     1201 gtcaagcagc tgcagctttt cccggtcaat tccaagcttc ggcgccatcg aaaggaacag
     1261 ctccgtcgcc tcccgcgaca gcttttccat acagatgacc agccgttccg gctcccatct
     1321 tctgtcgggg ccgcatgcca gtcgtatcag ttttttcaga tagagggtca gcagttcctc
     1381 cgccggatag gtctccccat ccacaaaaac cttctcaccg ccgaccgccc gcgagagaag
     1441 tctgccgttc gcctcctcgc cctgtgcaat ggcaagccgc actgcctcct cgccgacaaa
     1501 ccactgcccg attcctttct tcttggtaat cagcgccgga atctgataga tttcgctccc
     1561 tgcaatcgca gaaaaggtct ccggttcttt cttattcaat tcatagctgc tgatcacagc
     1621 gtactggtca tccagatcga ttcccagata aaaccggtgt tccatatgtt cccctctctg
     1681 tgctacaata gccggtatgc ctcttccgtc acttttgtca tgccataata gcgcttcatc
     1741 ttcccggcaa gcttctctct gtcatcgagc gtacacgcca gcagcatctc attcagccat
     1801 gcgtagcggt ctccgctgcc ctcttccata atatccccgt tgcccaggca gccgctcgcc
     1861 ccgatctgcc cgtccgcggt ctggctctcc aggatataat actgaacctt ctccccgaag
     1921 aacaggataa agtcttttac atagattccg tcatacattt ccgtcaattc atccgcaaga
     1981 tattcctgct gtgcctccag ccggtaatga acctcgatct gctgccccgg cgcggtgtga
     2041 tattccagaa agaacttatc tgtaagctga tatttgcgca gcagcttttc gtcgaacttc
     2101 cggaaaaatg caaaatacag attatgccgg atgcaggtgt caagaagctc gtccgcgatc
     2161 tttctgtcac gctccgacag gtgattctcc gagagatatt ttagcagtgc aatccggcag
     2221 acgcggttta gatcgccccc ggtcagataa cgctccctga tctggcggaa cacatgctcc
     2281 ggcacgacgg cgtcctccgt gagatacata ttggcaaaat aagagagata cgccatgcag
     2341 accagatctc tgccgccccc tgcgcaataa ctttcgtaga tctcttccat atgcggcgtg
     2401 taatcggtcg tatagagcat ctgtgtcaga atccgctctt ccagatcaaa cgtgtcgatc
     2461 tgaaattcgc ccgcagcttt ccacaattcc gccatctgct tggtcggccc attaaaatac
     2521 ttgcagaggt aaatcagcat gacatcgctg tattttccct gccggaaggt gctctccgca
     2581 aatcccagca gaaaatcatc ctcctcatag ccgtgatccg cgatcgcata gctgcagagg
     2641 gagacgcgcc actccgtctt cagataatcg tagccgtcga tctgcaccat ctgataggcc
     2701 ttctcataga ggtggttctc cgtcagaagc gcggccatct gcctgcgcac actcacgtcg
     2761 gaaagtgcct ccgcgagctg ccccttcatc agatccagtt ccccctccga ataggaaaaa
     2821 tccctgccgt cttttttctc gtaagaaatc agcgcaagtg caagccggac g
//
LOCUS       DBMDQJ010000296         2866 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12459_length_2866_cov_1.097649, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000296 DBMDQJ010000000
VERSION     DBMDQJ010000296.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12459_length_2866_cov_1.097649"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="ACZO9R_06855"
     CDS             <1..720
                     /locus_tag="ACZO9R_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0912819.1"
                     /translation="KFTWKNYIMVLQKDNLITGAYVTVARTVIGTVLALAANAILAFV
                     VSRKRFLFKRSLSLFWVITMYVNGGMIPTFLLYKGLGLTNSFWVYVIPGMVSAFNMLV
                     IRTYMNGISDSLEESAQLDGAGYTTIFLKIYCPLCKPVFATVALFVAVGQWNSWFDAM
                     LYNRMNSDLTTLQYELMKLLSSVTNQGTSAEAMKNAVGTVTPTSVRSAATILTMLPII
                     CIYPFLQKYFVTGLTLGGVKE"
     gene            810..2447
                     /locus_tag="ACZO9R_06860"
     CDS             810..2447
                     /locus_tag="ACZO9R_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 periplasmic-binding domain-containing
                     protein"
                     /protein_id="HIR0912820.1"
                     /translation="MKKRLAEGMTLMVLLLVLWNMAAHTDRMAEAQIKHFTAFFPTEG
                     NSLEYGNIMKAKIAEVTGAECEELWLSGQTKEQALNSYIARGRYPDFIQGEKNLYEAE
                     ALIPIDEYWEEYPNIRQYMTNEQWDMLRQEDGHIYWIPQFGVTNGKSSDVIHDGEAFW
                     IQTRVLKWAGYPEVHTVEQYFDLLEAYVNANPMMENGTRNIPFTVLSDDWRYFCLENV
                     PQFLDGYPNDGCCMVDPQTFQVMDYNTTETARAYFEKLNEEYRKGILDAEAFTNSYEE
                     YLNKLSTGAVLGMVDQWWQFYYAIYPAYDRQKLSEQGCDYVPLPITMQEGVENQWHVE
                     RAASIDTSSGLSVTVSCEDIDGALQFVNDLLKPEVEILRFWGQEGTDYCVDEEGVFYL
                     TKEQSKRFSDDLLASDYFCYYSYFPRWEGMLPDGKNAFSLEYQPAEFLKSQPGDVQKC
                     LRAYGCESYVDMIGNHKAPGNWYPMYSYATSLSDTTEAGMVYEQINEIKHEWLPRVIM
                     AEDFDTAWQEYMDAYNACSPQILMEDLQQEVMRRICR"
     gene            2506..>2866
                     /locus_tag="ACZO9R_06865"
     CDS             2506..>2866
                     /locus_tag="ACZO9R_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="early set domain-containing protein"
                     /protein_id="HIR0912821.1"
                     /translation="MGTELNQALVQQALSFAPEITEERQAVKVWEDGTVEFYLYAPTA
                     ETVEVAGVGGYFDAAPLALLPDGNGGFYRKIENFPRGMHYYHWFVDGVKLFHPKAGFS
                     YGCFETINTFEVPERGAE"
BASE COUNT          779 a          557 c          812 g          718 t
ORIGIN      
        1 aaatttacat ggaaaaatta tatcatggta ttgcagaaag ataatctgat taccggtgca
       61 tatgtcacgg ttgcgcgaac agttatcgga acagtgctgg cactggctgc aaacgcgatt
      121 ctggcgtttg ttgtcagcag aaaaagattt ctgtttaaga gaagcctttc cctgttctgg
      181 gtcattacaa tgtatgtgaa tggaggaatg attccgacat tcctgcttta caaaggactc
      241 ggtcttacca atagtttctg ggtgtatgta attccgggca tggtaagtgc gtttaatatg
      301 ctggtaatcc gcacttacat gaatggtata tcggacagtc tggaagaatc cgcacagtta
      361 gacggtgccg gatatacgac aatttttcta aagatttact gtccgctctg caagccggta
      421 tttgcgacgg tggcattgtt cgttgcagtc ggacaatgga attcctggtt tgatgccatg
      481 ctgtacaacc gcatgaattc tgatctgacg acgctgcagt atgagctgat gaagctgctg
      541 tcatccgtca cgaatcaggg aacttcggca gaagccatga agaatgcagt gggaaccgtt
      601 acaccgacgt cagtccgctc tgcggcaacg atactcacga tgctgccgat tatctgcatc
      661 tatccgtttt tacagaaata ttttgtcacc ggtcttacac tcggtggcgt aaaagaataa
      721 ttccatataa aagcgaagga gcgtatgcaa tcccatcttg ccggtgtccc ttaaaaacag
      781 tataattgac tggaaaagag ggaaaacaga tgaaaaagag actggcagaa ggaatgacct
      841 taatggtact gcttctggtg ctgtggaaca tggcggcaca cacagaccgc atggcagaag
      901 cgcagataaa gcattttacg gctttttttc cgacggaggg aaacagcctt gaatatggaa
      961 atatcatgaa agcgaagatt gcagaggtga caggagcaga gtgtgaagaa ctctggctgt
     1021 ccggtcagac caaggagcag gcactcaatt catacattgc ccgcggcaga tatccggact
     1081 tcatacaggg ggaaaaaaac ttatacgaag cagaggcatt gattccgatt gatgagtact
     1141 gggaggaata tcccaatatc cggcaataca tgacgaatga gcagtgggat atgctgcgcc
     1201 aggaagacgg acacatttac tggattccac aattcggggt gacaaatgga aaaagcagcg
     1261 atgtgatcca tgacggggaa gcattctgga ttcagacgag ggttttaaaa tgggcaggat
     1321 atccggaagt tcatacggta gagcaatatt tcgatctgct ggaggcttat gtgaatgcca
     1381 atcccatgat ggagaacgga accaggaata tcccatttac ggttcttagc gatgactgga
     1441 gatatttttg cctggagaat gtgccgcagt ttctggatgg atatcccaat gacggctgct
     1501 gcatggtcga tccgcagacc tttcaggtga tggattacaa cacgacagag acggcgcggg
     1561 cttattttga aaaattaaat gaagaatatc gtaagggaat actggatgcg gaggcgttca
     1621 caaacagcta tgaagagtat ctgaataagc tgtccaccgg agcggtcctt ggaatggtgg
     1681 atcagtggtg gcagttttac tatgcgattt atccggcata tgacagacag aaattgagtg
     1741 agcagggatg cgattatgta ccgcttccga ttacgatgca ggagggggtg gagaaccagt
     1801 ggcatgtcga gagagcggca tctatcgata cgtcctccgg cctgtcggtt acggtctcgt
     1861 gtgaagacat agacggtgcc ttgcagtttg tgaatgatct tttaaagccg gaggtggaga
     1921 ttctgcgttt ctgggggcag gaaggaacgg actattgcgt ggatgaggaa ggggttttct
     1981 atctgacgaa ggaacagtca aagcgttttt cggatgatct gctggcatcg gactatttct
     2041 gctattactc gtatttccca cgttgggagg gaatgcttcc ggatgggaaa aatgcttttt
     2101 cactggaata ccagccggca gaatttttaa aaagccagcc gggggacgtc cagaaatgtc
     2161 tcagggcgta tggctgtgaa agctatgtgg atatgattgg gaatcataag gcaccgggaa
     2221 actggtatcc gatgtattct tacgctacat cgctttctga taccacagaa gcaggaatgg
     2281 tatatgagca gatcaatgag atcaagcacg agtggcttcc gcgggttatt atggcagagg
     2341 attttgatac agcctggcag gaatacatgg atgcatacaa tgcctgcagt ccacagattt
     2401 tgatggagga tctgcagcag gaagtgatgc gccgcatctg ccggtagaac ctttcattct
     2461 atacgatatt ctcacagaag atcttataga atgggaggtt tttttatggg gacagaactt
     2521 aaccaggcgc tggtgcagca ggcactttcg tttgcgccgg agataacgga agaacggcag
     2581 gcggtgaagg tgtgggaaga tgggacggta gaattctatc tgtatgcacc gacggctgag
     2641 acggtggaag tggcgggtgt gggcggttat tttgatgcgg cgccgcttgc actcctgccg
     2701 gatggaaacg gagggtttta caggaagata gagaacttcc cgcgaggaat gcactattat
     2761 cactggtttg tggacggagt gaaactgttt catccgaaag ccggtttttc ttatggctgc
     2821 tttgagacaa tcaacacatt tgaggtgccg gagcgcggag cagaat
//
LOCUS       DBMDQJ010000297         2852 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12531_length_2852_cov_1.477644, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000297 DBMDQJ010000000
VERSION     DBMDQJ010000297.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12531_length_2852_cov_1.477644"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="ACZO9R_06870"
     CDS             complement(<1..869)
                     /locus_tag="ACZO9R_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002575197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HIR0912822.1"
                     /translation="MEYRNFFALLGGLALFLYGMHQMSEGLNAAAGEKMKQILERLTK
                     NRFLGVGVGAAVTAVIQSSSATTVMVVGFVNSGMMTLRQAVWIIMGANIGTTITGQLI
                     ALDAGAVAPLLAFLGVVLILFAGKDRIRYTGEIVAGLGILFIGMDMMGEAMQPLCDSQ
                     VFLQAMTKFEHPLLGILAGAAFTAAIQSSSASVGILQTLAQGGAVSFAGAVYVLFGQN
                     IGTCITAVLAAIGTGRNAKRTTIIHLSFNLIGTVVFTVLCMTTPLASLVAQLTPGRPA
                     AQIANMHTIFNIGT"
     gene            complement(900..1355)
                     /locus_tag="ACZO9R_06875"
     CDS             complement(900..1355)
                     /locus_tag="ACZO9R_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912823.1"
                     /translation="MRSADRRKAFFAVCMMAVLLFSHLGVCLWQTQIARMQNLTAEAG
                     GEAQGILRTTDSDYVSNALSAEPEAGLSQSMIVVTRKMTGAAEQRLFFHLFTAVLFKI
                     AEIPYAEAVVLTATAACICLAGYRMVRFVHNSDGKKDLPFWLSQVSEAS"
     gene            complement(1408..1845)
                     /locus_tag="ACZO9R_06880"
     CDS             complement(1408..1845)
                     /locus_tag="ACZO9R_06880"
                     /inference="COORDINATES: protein motif:HMM:NF047172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0912824.1"
                     /translation="MLEDFFENYFKIEKAYRKYFQDEMEKYHLSPNELLVLLFLARDN
                     GGCNTARDIAQYEGVSKGLVARSVESLVEKQFLVVERDAADKRICHLYLTQQCSVLTK
                     QITEKKAAFFRRLAEGIPEEAIATTEQTLRRFMENISASIDKS"
     gene            2086..>2852
                     /locus_tag="ACZO9R_06885"
     CDS             2086..>2852
                     /locus_tag="ACZO9R_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HIR0912825.1"
                     /translation="MNKKLKEKFDESVSAVLPITVIVLLLSIFVVPMETGTIALFLVG
                     AFMLIIGMAFFQLGAEMAMTPLGEGGGSHIMKTKRLSILIIVCFVMGVIITIAEPDLQ
                     VLANQVPSIPNETLIWTVALGVGVFLVIAVLRIIFKLSLPLLLCILYAALFLLSFAAP
                     ADFIAVAFDSGGVTTGPMTVPFIMAIGVGLSSTRSDKDGSSDSFGLISFCSIGPIAMV
                     LLLGIFYHPAGASYNATEIPDVVTMQDVMRQFVHMLPG"
BASE COUNT          670 a          802 c          630 g          750 t
ORIGIN      
        1 gttccgatat taaaaatcgt atgcatgttg gcaatctggg cagccggccg tcccggcgtc
       61 agctgcgcaa ccagcgaagc gagcggtgtt gtcatgcaca gcacggtaaa caccaccgtt
      121 ccgatcagat tgaacgaaag atgaatgatc gtggtgcgct tggcgtttct tccggtgcct
      181 atggcagcaa gaaccgccgt aatgcatgtg ccgatattct gaccgaacag aacatacacc
      241 gcccccgcga aagatacggc tcctccctgt gccagggtct gcaggatacc gaccgaagcc
      301 gaggatgact gaatggctgc cgtgaacgcc gcgcccgcca ggattccaag cagtggatgt
      361 tcaaactttg tcattgcctg aaggaacacc tgcgaatcac agagcggctg catggcttcc
      421 cccatcatat ccatgccgat aaacaggatt ccaagccccg ccacaatctc gcctgtgtac
      481 cggatccggt cttttcccgc aaacagaatc agaaccaccc ccaaaaaagc cagaagcggc
      541 gcaacggctc ccgcatccag tgcaatcagc tgaccggtga ttgtggtgcc gatatttgcc
      601 cccatgataa tccagaccgc ctggcgcaga gtcatcatcc cggaattcac aaagccaacg
      661 accatgaccg tggtggctga ggatgactgg atcaccgccg tcaccgctgc tccgacgccc
      721 acgccaagga agcggttctt tgtcagccgc tccaggatct gcttcatctt ctcacctgcg
      781 gcagcattta agccctcgct catctgatgc attccataca aaaagagtgc aagcccgccc
      841 agcagtgcaa aaaaatttct atattccatt ttcttctttt cccttttctt atcctaatct
      901 tatgacgctt ctgatacctg tgataaccaa aagggcaaat cttttttacc gtcggaattg
      961 tgcacaaaac gcaccatgcg gtaacctgcc aggcagatac acgccgccgt ggcggtaagc
     1021 actaccgcct ctgcataggg aatctctgct attttaaata aaacagcagt aaaaagatga
     1081 aaaaaaagac gctgctccgc cgctccggtc atttttctcg tcacgacaat catactctgt
     1141 gacaatcctg cttccggttc cgctgagagc gcgtttgaga cataatcgga gtccgtcgtc
     1201 cgtaaaatac cctgtgcttc ccctccggcc tctgccgtca gattctgcat ccgggcgatc
     1261 tgtgtctgcc acagacagac accaagatgg gaaaacagca gaaccgccat catgcaaacc
     1321 gcaaaaaatg cttttcttct atctgcactg cgcatgttcc ctgctccttt cccattccaa
     1381 gactctttcc tcacgatagc aaagcactta cgacttgtca atagatgcgg atatattttc
     1441 cataaacctg cgcagtgtct gttccgtcgt cgcaatcgct tcctccggaa tgccttcggc
     1501 cagcctgcgg aaaaacgcgg cttttttctc cgttatctgc ttcgtgagca cactacattg
     1561 ctgcgtgaga tataaatggc agattctctt atcggcagca tcccgttcca ccacaagaaa
     1621 ctgcttttcc acgaggctct ccaccgatct ggcaaccagt cccttggaca ccccctcgta
     1681 ctgcgcaata tcccgcgccg tattgcatcc gccgttgtcg cgtgccaaaa aaagaagcac
     1741 cagcaattcg ttcggcgaga gatgatattt ctccatctca tcctgaaagt atttccggta
     1801 tgctttctct atcttaaaat aattttcaaa aaagtcctcc agcatgttcc ccctcctttc
     1861 cgccggttca tgccttctct ttgctttagt tgatttgtga actgtttata tagaaacttt
     1921 atttgaaatc tgtataaaag tcaattaaaa aagtgttaaa atttcagaaa aatcactccg
     1981 actcggttga ctttctacac aacaaccgat aaaataatag ttgatttgtg aactgtttaa
     2041 ctggtaactc tattaaccga atcctagaaa gtgtgtgatg ttattttgaa caaaaaatta
     2101 aaagagaaat tcgacgaatc tgtaagcgcc gttcttccca ttaccgtcat tgtgctgttg
     2161 ctcagcattt ttgtcgtacc gatggagacc ggaaccattg ccctgtttct ggtcggagcg
     2221 ttcatgctga tcatcggcat ggcatttttc cagctgggtg ctgagatggc gatgacaccg
     2281 ctcggcgaag gtggcggttc ccatatcatg aagacgaagc gtctttcgat tctgattatt
     2341 gtctgctttg tgatgggtgt catcatcaca atcgcggaac cggatctgca ggttctcgcc
     2401 aatcaggttc cttctattcc aaacgaaacc ttaatctgga cggtcgctct cggcgtcggt
     2461 gtctttctgg tcatcgctgt tttgcgtatc attttcaagc tgagcctccc cctgctgctg
     2521 tgtatcctct atgccgcact atttttgctc tcctttgcgg cgccggctga tttcatcgca
     2581 gtcgcatttg actccggcgg tgtgacgaca ggtccgatga ctgttccttt tattatggcc
     2641 atcggtgttg gtctctcctc cacgcgaagt gacaaggacg gttcgagcga cagtttcggt
     2701 ctgatctcct tctgcagcat cggaccgatt gccatggtac tcctgctcgg tattttctac
     2761 catccagccg gtgcatccta caatgccacc gaaataccgg atgttgtgac catgcaggat
     2821 gtcatgcgtc agtttgtaca tatgctgccg gg
//
LOCUS       DBMDQJ010000298         2850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12545_length_2850_cov_1.343034, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000298 DBMDQJ010000000
VERSION     DBMDQJ010000298.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12545_length_2850_cov_1.343034"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="ACZO9R_06890"
     CDS             complement(<1..233)
                     /locus_tag="ACZO9R_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR0912826.1"
                     /translation="MYNKVDTNLNFVDREKQVREFWKDNDIFKKSMEQRKQGETYTFY
                     DGPPTANGKPHIGHVETRTIKDMIPRFQAMKGK"
     gene            complement(701..1678)
                     /locus_tag="ACZO9R_06895"
     CDS             complement(701..1678)
                     /locus_tag="ACZO9R_06895"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR0912827.1"
                     /translation="MTKFEELLQQINRQHVYIQTHNFPDPDAIASAYGLKRLLATRGI
                     EASICYKGKIDRYGTGRLCEILDIELLNVEDLTEVLTDEDEVILVDSQKGNSNIINIT
                     GDEIICIDHHPYNDKCQYRFQDIRPEVGACATMIAQYFMENDIAMDKKIATALTYGIR
                     IDTHNLSRGVSKLDIEMLYRMYDDCDYEEIHTLENSNLCFEDLVAYSKAISSIEVYDN
                     ISFADTGEDCPEALIASVSDFMLALVEVSFSVVYSRRGDGIKLSVRSEKSSLDAGKIT
                     AEALQGIGNGGGHAAMAGGFVPFTGSDGKAVILIDQIKERFFSTIAKRR"
     gene            complement(1756..2418)
                     /locus_tag="ACZO9R_06900"
     CDS             complement(1756..2418)
                     /locus_tag="ACZO9R_06900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0912828.1"
                     /translation="MKRGIIFDMDGTLWDSAENVAKSWNVAIRQYGVREKELTTQDIQ
                     GVMGKTMDVIADILFAEFPEEKRRLLLKECCQVENDYLREHGGILYDQVPQTLAALKR
                     DGYHLSIVSNCQKGYIEAFLDHYDLWELVEDIECYGNNLRQKGENIRLVVERNRLDEA
                     VYVGDIQGDYDASRAAGVGFIHAAYGFGEIAEPVPELKTFADLKTFDISSASKTQSAW
                     TM"
     gene            complement(2448..>2850)
                     /locus_tag="ACZO9R_06905"
     CDS             complement(2448..>2850)
                     /locus_tag="ACZO9R_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HIR0912829.1"
                     /translation="DHVPVEKLPELWNQKMQEYLHITPENDAEGILQDVHWSDGSFGY
                     FPSYLLGSIYDGMYLEKMEEELGSVDEILADGRIGEITKWLNEKIHWYGSTRTPKEVI
                     AAVCGKEVSAQPLVRYMKKKYTRLYGLSEEA"
BASE COUNT          592 a          863 c          606 g          789 t
ORIGIN      
        1 tattttccct tcattgcctg aaatctcggg atcatatcct tgatcgtacg cgtctccaca
       61 tgaccgatat gcggcttgcc gttggcggtt ggtggtccgt catagaacgt gtaagtctcg
      121 ccctgcttgc gctgctccat acttttcttg aagatgtcat tgtctttcca gaactctctt
      181 acctgctttt ctctgtccac aaaattcagg ttcgtgtcta ctttgttata cattttttcc
      241 tccaaaataa aacatttaag gctcccgtcc tgtaaatagg acgagagcct ttatcgttat
      301 accacctaat tacattgtac acaaacttct ccacgcacag aaaccctggg tgtgcttctg
      361 cagcttgaaa aaatctcatc cggagaaaat ctggataccc gttcccttta acgcaggaaa
      421 agcgtcagcg cctaccgtgc tgccttcctg tctgacacag gctctgctgt gccacatctg
      481 cggctgcatt tcgggctgcg gcttggaagg gatcttcgtc tgcggcttac tcgcaccggc
      541 tctcaccgcc ccggctcgct ttgcctttca tccacagctt actgtcttcg tcactgcctt
      601 tgtcaactat gtgtaagtat aagccttgca agcccgcatt gtcaaggtct gaaatgccgt
      661 ttcccgtctc atccggcgcg cctttcgtgc accgtctgtg ctatctccgc tttgcaatcg
      721 tcgaaaaaaa acgctccttg atctggtcga tcaaaatgac cgccttgccg tccgaaccgg
      781 taaacggtac aaagccacct gccatcgcag catgcccgcc gccgtttccg atcccctgca
      841 gcgcctccgc cgtgatcttg ccggcgtcga gagaagattt ttcactccgg acggacagtt
      901 tgatgccatc ccctctcctt gagtacacca cggaaaaaga aacctccacc agcgccagca
      961 taaagtcaga gacactcgcg atcaatgcct ccgggcagtc ctcccccgta tcggcaaagc
     1021 tgatattatc gtacacctca atgctggaaa tcgccttgga atacgccacc agatcctcaa
     1081 aacacaggtt gctgttctcc agtgtgtgga tctcctcata atcacagtca tcgtacatcc
     1141 gatagagcat ctcgatatcg agctttgaca cgccgcgtga aagattgtgc gtgtcaatgc
     1201 ggatgccgta tgtcagtgct gttgcgatct ttttatccat cgcaatgtca ttttccataa
     1261 aatactgcgc gatcatcgtt gcgcacgccc cgacttcggg gcggatgtcc tgaaaccggt
     1321 actgacactt atcattgtaa ggatgatggt cgatgcagat gatctcatct cccgtaatat
     1381 tgatgatgtt ggaattgccc ttctgggaat ccaccagtat cacttcgtcc tcatccgtca
     1441 gcacttcggt cagatcctcc acattcaaca gttcaatgtc aagaatctca cacagacggc
     1501 cggtaccgta ccggtcgatc ttgcccttat agcagatgct cgcctcgata cccctggttg
     1561 caagcagccg cttcagacca tatgcacttg caatcgcatc cggatccggg aaattatggg
     1621 tctgtatata gacatgctgt ctgttgatct gctgtagtaa ttcttcaaac tttgtcatgc
     1681 tttctctctc ctgcatgcgg ctgccggctc acatggttcc ggcgctctgt tctctggctg
     1741 cccggtgctg ccggctcaca tggtccaagc gctctgcgtt ttgctcgccg atgaaatatc
     1801 aaaggttttt aggtctgcaa aggttttcag ttccgggacc ggctccgcaa tctctccgaa
     1861 tccatatgcg gcgtggataa acccgactcc tgccgcccgg ctcgcatcat aatctccttg
     1921 aatgtctccg acatagaccg cctcatccag ccggttccgc tctacgacca gccggatatt
     1981 ttctcccttc tgccgcagat tattgccata acactctatg tcctccacca gttcccacaa
     2041 atcgtagtgg tcaaggaatg cctcgatata ccccttctgg cagttgctga caatcgaaag
     2101 atgatagccg tcccgcttca acgccgcgag cgtctgcggc acctgatcgt agagtatgcc
     2161 cccatgctcc cgcagataat cattttccac ctgacagcat tcctttagga gcagtctccg
     2221 tttttcttcg gggaattccg caaagaggat atcggcgatt acgtccatcg tttttcccat
     2281 taccccctga atgtcctgcg ttgtcagttc tttctccctg accccgtact gccggattgc
     2341 cacattccag gacttcgcca cattctctgc ggaatcccac agggttccgt ccatgtcaaa
     2401 tatgattcca cgtttcatga catcatctcc cgttctctct cttttgtcta ggcttcctcg
     2461 gaaagtccat acagtctcgt gtattttttc ttcatgtagc gcacgagcgg ctgcgcggac
     2521 acttctttcc cgcacactgc cgcaattacc tctttcggtg tccgcgtgct tccataccag
     2581 tgaatcttct cattgagcca ttttgtgatc tcaccgattc tgccgtctgc caggatctca
     2641 tccacggagc cgagttcctc ctccatcttc tcgaggtaca tgccgtcata gatgctgccc
     2701 agcagatagg acggaaaata cccaaacgag ccgtccgacc aatgtacatc ctgcaagatg
     2761 ccttcggcgt cattttccgg agtgatatgc aagtattcct gcattttctg attccagagc
     2821 tccggaagtt tttccaccgg cacatgatcg
//
LOCUS       DBMDQJ010000299         2836 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12625_length_2836_cov_1.654479, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000299 DBMDQJ010000000
VERSION     DBMDQJ010000299.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12625_length_2836_cov_1.654479"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            32..1336
                     /gene="mtaB"
                     /locus_tag="ACZO9R_06910"
     CDS             32..1336
                     /gene="mtaB"
                     /locus_tag="ACZO9R_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035598 - tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HIR0912830.1"
                     /translation="MKAALHNLGCKVNAYETEAMQQILEEAGYEIVPFSEYADVYVIN
                     TCSVTNMADRKSRQMLHRAKKQNPDAIVVGAGCYVQTKEAQALVDESIDIVIGNNKKH
                     ELVPLLREYEASHRKMACVADINHEKQAYEELSLSRTAEHTRAFIKVQDGCNQFCTYC
                     IIPFARGRVRSRELPDVLQEIRTLAKSGYREVVLTGIHLSSYGVDNGESLLHLIEAVH
                     ELEGIERIRLGSLEPRIVTDAFAKRLSELPKICPHFHLSLQSGCDAVLSRMNRRYDTK
                     EYEAGCALLRRYFEHPAITTDVIVGFPGETDEEFETTERYLERIHFYEMHIFQYSRRE
                     GTKAAAMPDQVPETVKKERSEKLLALGHKMSEEFRRYYLGRQVTALLEEEFFHDGKRY
                     YTGYTKEYVKVAVETEKDLSNTFVTGTLKTQLTEDVYLLVEF"
     gene            1439..1702
                     /locus_tag="ACZO9R_06915"
     CDS             1439..1702
                     /locus_tag="ACZO9R_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HIR0912831.1"
                     /translation="MQEINNTQYFKVEKGPELRVSDVLAIVYRALMEKGYNPVNQIVG
                     YIMSGDPTYITSHNNARSLIMKVERDELVEEMLKAYIRNNNWE"
     gene            1706..2137
                     /gene="ruvX"
                     /locus_tag="ACZO9R_06920"
     CDS             1706..2137
                     /gene="ruvX"
                     /locus_tag="ACZO9R_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR0912832.1"
                     /translation="MQILGLDFGSKTVGVAVSDELMLTAQGLEIVRRQSPNKLRQTLA
                     RIEQIVTEYQVERIVLGYPKNMNNTEGERCEKTKEFAQMLERRTGLSVVLWDERLTTV
                     AADRHMMESGIRRENRKQYVDEIAAVFILQGYLDSLAFQKQ"
     gene            2149..2406
                     /locus_tag="ACZO9R_06925"
     CDS             2149..2406
                     /locus_tag="ACZO9R_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HIR0912833.1"
                     /translation="MEKVKFMDPDTGESLAFFVVEETKVGGTHYLLATEEEDGDCDAY
                     ILKEVETKDEESVYVLVEDDTELSAIGKVFAELLDDADIAF"
     gene            2434..>2836
                     /locus_tag="ACZO9R_06930"
     CDS             2434..>2836
                     /locus_tag="ACZO9R_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0912834.1"
                     /translation="MKKEKKENSGKLKIIPLGGLEQIGMNITAFEYEDSIIVVDCGLS
                     FPEDDMYGIDLVIPDITYLKDNIDKVKGFFITHGHEDHIGAIPYVLREINVPIYSTKL
                     TNGIIEHKLREHNMLTKVKRKVVKYGQHINLG"
BASE COUNT          832 a          542 c          832 g          630 t
ORIGIN      
        1 caaaaagcaa tacagaaatg aggaaacaag aatgaaagca gcattgcata atttgggttg
       61 taaagtgaat gcatatgaga cggaagccat gcagcagatt ttggaggagg cgggatatga
      121 gatcgtgccg ttttcggagt atgcggatgt ctatgtgatc aatacatgct ccgtgaccaa
      181 tatggcggac cgcaagtcca gacagatgct gcaccgggcg aaaaagcaga atccggatgc
      241 gattgtcgtg ggagccggat gctatgtgca gaccaaggag gcacaggctc tcgtggatga
      301 gtcgattgac atcgtgatcg ggaataataa aaagcatgag ctggtgccgc tgcttcggga
      361 atacgaagca tcccaccgca agatggcgtg tgtggcggac atcaatcacg agaagcaggc
      421 atacgaggaa ttgtcgcttt cgcgcacggc agagcacacc cgggcgttta tcaaggtgca
      481 ggatggctgc aatcagttct gcacatactg tattattccg tttgcgagag ggcgtgtcag
      541 aagcagggag cttccggacg tcttacagga gatacggacg ctggcaaaaa gcggctaccg
      601 cgaggtggtg ctcaccggaa tccatctgag ttcctacggt gtggacaacg gggagtcgct
      661 gctgcatctg atcgaggcgg tacatgagct ggaggggatc gagcggatcc gcctgggcag
      721 tttagagccg cgcattgtga cggatgcatt tgcaaaaaga ctttcggagc tgccgaagat
      781 ctgtccgcat tttcacctgt cactgcagag cggctgcgat gcggtgttaa gccgcatgaa
      841 ccggcggtat gatacgaaag aatatgaggc gggctgcgca cttttgcgca ggtattttga
      901 acatccggca atcacaacgg atgtgattgt agggttcccg ggggagacgg acgaggaatt
      961 tgagacgacg gagcgttatc tggaacggat tcatttctat gagatgcata ttttccagta
     1021 ttcgagaaga gagggaacga aggcggcggc aatgccggat caggtgccgg agacggtaaa
     1081 aaaagagcgc agcgaaaaac tgcttgcact cggtcacaag atgtcggagg agttccgccg
     1141 gtattacctc ggtagacagg tgacagcgct tctggaggaa gagtttttcc acgacggaaa
     1201 gcgttactac accgggtaca caaaagagta tgtgaaagtg gcggtggaga cagaaaaaga
     1261 cctttccaac acgtttgtga cgggcacctt aaagacacaa ttaacagaag acgtctatct
     1321 tttagttgaa ttttaagaca aactatatta gaataaaaac agcaatgctt tttcgacaag
     1381 agagcggtgg ctggcagcac cgctttaaag tccgggcaga tataagggcg tgacaaatat
     1441 gcaggagatc aataacacac aatattttaa agtggaaaaa ggaccggaac ttcgcgtgag
     1501 cgacgtgctc gcgattgttt atcgtgcgct tatggagaag ggatacaatc cggtgaatca
     1561 gattgttgga tatattatgt cgggagatcc tacttatatc accagtcata ataatgcaag
     1621 aagcctgatc atgaaagtag aacgtgatga gctggtagag gagatgctga aggcttacat
     1681 caggaataac aactgggaat agaagatgca gatattaggt ttggattttg gctcgaagac
     1741 tgtcggtgtg gcagtcagtg atgaacttat gttgacggcg cagggactgg agatcgtgag
     1801 aagacagtct ccgaataagc tgcgccagac gttggcaaga atagaacaga ttgtaacaga
     1861 atatcaggtg gagcgcattg tgctcggcta tcccaagaat atgaacaata ccgagggaga
     1921 gcgttgcgag aagacgaaag agtttgcaca gatgctggag cgccgcaccg ggctttctgt
     1981 cgtcctgtgg gacgagagac ttacgacggt tgcagcagac cggcacatga tggagagtgg
     2041 aatacgcaga gagaatcgca aacagtatgt ggatgagatc gcggcagtgt ttattctgca
     2101 gggatatctg gattcactcg cgtttcagaa acagtgaatg gagtaaatat ggaaaaagta
     2161 aagtttatgg atccggacac cggggaatcg cttgcgtttt ttgtggttga ggagacaaag
     2221 gttggcggca cgcactacct tctggcgacg gaagaggaag acggcgactg tgatgcgtat
     2281 attttaaaag aggtggaaac gaaagacgag gaatctgtct acgtgcttgt ggaagacgac
     2341 acggagcttt ctgccatcgg aaaagtattt gcagagctgc tcgacgacgc ggacattgca
     2401 ttttaagaga caaataataa tggaggtaat attttgaaaa aagaaaaaaa agagaatagt
     2461 ggaaagctca aaatcattcc gttaggagga ttggagcaga ttggtatgaa cattactgcc
     2521 tttgaatatg aggacagtat tattgttgtg gactgcgggc tgtcttttcc ggaggatgac
     2581 atgtacggaa ttgatctggt catcccggat atcacctatc tgaaggacaa cattgacaag
     2641 gtcaaaggat tttttatcac acacggtcat gaggaccaca tcggagcaat cccttatgtg
     2701 ttaagagaga tcaatgtgcc gatctattcc accaagctga cgaacggtat cattgagcac
     2761 aagttaagag aacacaatat gctcaccaag gtaaagcgca aggttgtaaa atacggacag
     2821 catatcaatc tcggct
//
LOCUS       DBMDQJ010000300         2833 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12637_length_2833_cov_1.762079, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000300 DBMDQJ010000000
VERSION     DBMDQJ010000300.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12637_length_2833_cov_1.762079"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>628)
                     /locus_tag="ACZO9R_06935"
     CDS             complement(<1..>628)
                     /locus_tag="ACZO9R_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0912835.1"
                     /translation="NRYFAQMLSTISTEAYARGYGVNITLHGGDAGTEREQLTRLADY
                     RVDGIILSSVNEGEAYRTFLESLHRPIVSVDNRIADGIPFVGIDQRAAVREAVQRAAE
                     KGYERIVFVCPPLDTKNRQNLYVHKERLLGYEEEMKRYPALRPECILSWDYLQKVDAI
                     LKEGKRTAFVCTADEYALEIMKDQKRAKRKAPVDYGITGFDNIDTLAYV"
     assembly_gap    629..638
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<639..834)
                     /locus_tag="ACZO9R_06940"
     CDS             complement(<639..834)
                     /locus_tag="ACZO9R_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006860516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0912836.1"
                     /translation="MAITTKDLAQLCGVSRTTIHRALNGTGRIKPETKEMILQVAKEH
                     GYRPDLLATGLAKGQTYNIGV"
     gene            complement(878..2386)
                     /gene="arfA"
                     /locus_tag="ACZO9R_06945"
     CDS             complement(878..2386)
                     /gene="arfA"
                     /locus_tag="ACZO9R_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosylfuranosidase ArfA"
                     /protein_id="HIR0912837.1"
                     /translation="MRHVTIKADTAFTKGRIDDRIYGSFVEHMGRVVYTGIYEPSHKT
                     ADENGFRGDVLQKVRQMGVTAVRYPGGNFVSNYDWRDGIGPVEKRPRKRDLAWKSIET
                     NEVGLDEFMKWADAAEVAPIYAVNLGTKGIEEALAVLEYCNMETGTYYSDLRAANGHE
                     KPYGILTWCLGNEMDGDWQIGHKTAEEYGRIAYETGKAMKILDPSIELVACGSSMSTN
                     PTFGDWEQTVLEHCYPCLDYISLHQYYGGQEKGTAEFLAQSLDMERYIETVLNICDYV
                     RVKKKMSHKINICFDEWGVWSMSDVQVQKSVEDAPWQTAPAISEQIYTMEDALLFASM
                     LMNFIKRADRVKIACQSLLTNISSCIMTEEDGGVWVQPVYYPFALMAKYAHGVTLDTR
                     YTGDTYACSTFETVPCVDHVLVYDGQKHEVTVFFVNRMEESAEATVSLQGMEPEAVLK
                     SIALFHEDKKATNQKEHDCVTPVELENVSCSGNEISVELVPLSFQMVQIRLK"
     gene            complement(2405..>2833)
                     /locus_tag="ACZO9R_06950"
     CDS             complement(2405..>2833)
                     /locus_tag="ACZO9R_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0912838.1"
                     /translation="GILNTFLGLILPRAASAYGIFMLTSFFSGIPKSLDEAAKIDGMG
                     EWKIYLKIILPLAKPALVTLGIYHFMNNWNDLLYPMMLTSSVEMRTLSAGLAILVGSN
                     SIKYGPTLAATVIAILPLLLLFLFGQKFFMEGIATSGMKE"
BASE COUNT          617 a          862 c          598 g          746 t
ORIGIN      
        1 tcacatatgc cagcgtatcg atattgtcaa atccggtaat gccgtaatcc accggcgcct
       61 ttctttttgc ccgcttctga tccttcatga tctcaagtgc atactcatct gcggtacaga
      121 caaacgccgt ccgctttcct tcttttaata tggcatccac tttctgcagg taatcccagg
      181 agagaataca ctccggccgg agcgcgggat agcgtttcat ctcttcctca taccccagca
      241 gacgctcctt gtgaacatac agattctgcc ggttttttgt atctaacggc gggcagacaa
      301 acacgatgcg ctcatagccc ttctctgccg ctctctgcac tgcctcccgc actgctgccc
      361 gctgatcgat cccaacaaac gggatgccgt ccgcaatccg gttatccaca gagacaatcg
      421 ggcggtgcag ggactccagg aatgtccggt acgcctcccc ctcgttgacc gaggagagga
      481 tgatcccgtc aacccggtaa tccgcaagtc ttgtcagctg ctcgcgctcc gtcccggcat
      541 ccccgccgtg cagcgtaatg ttgacgccgt agccgcgggc atatgcctcc gtgctgatcg
      601 tcgagagcat ctgggcaaaa tagcggttnn nnnnnnnncg acaccgatgt tgtacgtctg
      661 tcccttagcc agtccggtgg caaggagatc cggccggtag ccgtgttcct ttgccacctg
      721 aaggatcatc tcctttgtct ccggcttaat ccgcccggtt ccatttaatg cccggtgtat
      781 cgttgttctg gaaacgccgc ataactgcgc cagatctttt gtcgtgatcg ccatattttc
      841 ctgctccccg ttttctttct tttcaaaaaa ctttcttcta ttttaatcga atctgaacca
      901 tttgaaaaga gagaggtaca agttccactg aaatttcgtt tccgctgcag gagacgtttt
      961 ccagttccac aggtgttaca cagtcatgtt ccttttgatt tgtcgctttc ttatcctcgt
     1021 gaaacagtgc aatacttttt aaaaccgcct ccggctccat gccctgtaaa gaaacggtcg
     1081 cctcggcact ctcctccata cggtttacaa agaaaaccgt cacttcatgc ttttgcccgt
     1141 catagacgag cacatgatcc acacagggta ccgtctcaaa cgtgctgcac gcgtaggtgt
     1201 ctcccgtata ccgtgtatct aacgtcacgc cgtgcgcata cttcgccatc agggcaaacg
     1261 gataatacac cggctgcacc cacacaccgc cgtcctcctc tgtcataatg caggagctga
     1321 tattcgtaag aagtgactgg caggcgatct tcacacgatc cgcccgtttt atgaaattca
     1381 tcagcatact tgcaaacaga agcgcatcct ccatggtata gatctgctcg ctgatcgcgg
     1441 gtgccgtctg ccacggtgca tcctccaccg atttctgcac ctgcacgtcg ctcatcgacc
     1501 agacgcccca ctcatcaaaa cagatgttga tcttatgtga cattttcttt tttaccctca
     1561 catagtcgca gatgtttaag accgtctcga tatagcgctc catatccagc gactgtgcca
     1621 gaaactctgc cgtgcccttc tcctgtcccc cgtaatactg gtgcagggaa atgtaatcga
     1681 ggcagggata acaatgttcc agtacggtct gttcccagtc accgaacgtc ggattcgtcg
     1741 acatcgaact tccgcacgcc accagttcga tggatggatc taagattttc attgcctttc
     1801 cggtctcata ggcgatcctg ccgtattcct ccgccgtctt atgcccgatc tgccagtctc
     1861 cgtccatctc attgcccaaa caccaggtca ggatgccgta cggcttttca tgtccgtttg
     1921 ccgcccggag atcactgtag tatgtgccgg tctccatatt acaatattcc agcaccgcaa
     1981 gcgcttcctc gattcccttg gttccaagat tcactgcata gatcggtgca acctctgccg
     2041 cgtccgccca cttcataaat tcatccagac cgacctcgtt tgtctcaata cttttccacg
     2101 ccagatcccg cttcctcggg cgtttctcca caggtccgat cccgtcccgc cagtcatagt
     2161 tggagacaaa attgcccccg ggatagcgca ccgccgtgac ccccatctgc cttaccttct
     2221 gcagcacatc gccccggaat ccgttttcat ctgcagtctt atgcgacggc tcgtagatgc
     2281 cggtgtacac cactcttccc atgtgctcca cgaaggagcc gtagatacgg tcgtcaatcc
     2341 tgccttttgt aaatgccgta tcagctttaa ttgtcacatg tctcatcttc tctctcctct
     2401 gcctctattc tttcattccc gatgttgcga ttccttccat gaaaaacttc tgtccgaaca
     2461 aaaacagcag taacagcggc agaatcgcaa tcaccgtagc agcaagtgtc ggaccatatt
     2521 tgatcgaatt acttccaacc agaattgcaa gtcctgctga cagcgtccgc atttccaccg
     2581 aacttgtcag catcatcgga taaagcagat cgttccagtt attcatgaaa tgatagattc
     2641 ccagtgtcac aagtgccggc tttgcaagcg gcagaatgat tttcagatat attttccact
     2701 ctcccatacc atcaattttc gcagcctcat ccagcgattt cggtatgccc gagaagaagg
     2761 aggtcagcat gaaaataccg taggcactcg ctgcgcgtgg cagaatcagt cctaagaacg
     2821 tatttaaaat tcc
//
LOCUS       DBMDQJ010000301         2832 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12642_length_2832_cov_1.571952, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000301 DBMDQJ010000000
VERSION     DBMDQJ010000301.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12642_length_2832_cov_1.571952"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            186..449
                     /locus_tag="ACZO9R_06955"
     CDS             186..449
                     /locus_tag="ACZO9R_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR0912839.1"
                     /translation="MEKIVVKVDGMQCGMCEAHVNEAVRAAFPVKKVTSSHAKGETVI
                     LTEQDIDDAKLKEVIDKTGYEVGTVTREPYEKKGLFSAFRHEK"
     gene            658..858
                     /locus_tag="ACZO9R_06960"
     CDS             658..858
                     /locus_tag="ACZO9R_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912840.1"
                     /translation="MRSRCSVWRRAKDASTLPVSLYLADRVSITGAQAALAVMLSMSL
                     VGSLAKLEVFFQNTRQMKMTAE"
     gene            1191..2486
                     /gene="eno"
                     /locus_tag="ACZO9R_06965"
     CDS             1191..2486
                     /gene="eno"
                     /locus_tag="ACZO9R_06965"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007591041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR0912841.1"
                     /translation="MNFLEIEKVIGREILDSRGNPTVEAEVTLADGTVGRGMAPSGAS
                     TGEFEALELRDGDKARYLGKGVTKAVENINTVINDTICGMDASDIYALDAAMIAVDGT
                     KDKSKLGANAILAVSIAAARAAAIAQDIPLYRFLGGISGNRLPVPMMNILNGGAHAAN
                     TVDVQEFMIMPVGAGSFKEALRWCAEVFHALAALLKSKGLATSVGDEGGFAPDLASDE
                     EAIEYILQAVKNAGYEPGKDFMIAMDAASSEWKGTKKGEYILPKAGTKFTSEELIEHW
                     KKLVEKYPIISIEDALDEEDWEGWQKLTKELGDKVQLVGDDLFVTNTERLKKGIDSGC
                     GNAILIKLNQIGSVSETLEAIKMAHKAGYTAISSHRSGETEDTTIADLAVALNTCQIK
                     TGAPSRTERVAKYNQLLRIEEELGDAAVYPGMAAFNVKR"
BASE COUNT          772 a          568 c          874 g          618 t
ORIGIN      
        1 tccacatttt atatgcctta aagatgggag aaaaataccg ggcgcgttat cacaggcatt
       61 ttgcgcaccc ggtactgcat gaactggaca tgcagcatga ggagcttctt gcagtacatc
      121 cgaaggaatg ggtggagctg atagaggata tatgcagaca gaagtaatac ggaggaagaa
      181 cagcaatgga aaaaattgtg gtaaaagtgg atggcatgca gtgcggaatg tgcgaggcac
      241 atgtgaatga ggcagtgaga gctgcgtttc cggtgaagaa ggtaacctct tcccatgcaa
      301 aaggggagac tgtcattctg accgaacagg acatcgatga tgcaaagtta aaggaagtca
      361 ttgacaagac cggctatgag gtgggaactg tgacgagaga accttatgaa aagaagggac
      421 ttttttccgc atttcgtcac gaaaagtaag aaaaaggatg tctggataga gcgccctgtc
      481 tggggtattc cgtttaatga ataggtgcga aggtgtgaaa ccggagcttt atttatgagg
      541 aatcccgggc agggcgtatt ttatgagttg ctgtttgtta tggtattgct gttctgacgc
      601 agagggcagc catgccgcac acggtaaaaa ggagagggac gatggagact taccggaatg
      661 aggagcagat gcagtgtctg gcgaagagcg aaggatgcaa gtacgcttcc ggtcagcctg
      721 tatctggcgg accgcgtcag cattaccggg gcgcaggcgg cgcttgcggt gatgctttcg
      781 atgtcgctgg tcggatcgct ggcaaagctt gaagtatttt tccaaaatac ccgtcagatg
      841 aagatgacgg cggagtagcg gtgcagtaag ggaaagggcg ttcttatccg gcggagaatc
      901 cgcaatgaga aaaagtatca acatccgggg ttgcaatctt tttcgccaac cgttataatg
      961 acgacagtta ggcgtgacta actatagtgc aaatcatttt aggacttctt acaggtgcgg
     1021 agctgctgaa tccgtttcct gccggaaaac agctgcaccg ggacgcgttc catcctatgt
     1081 aacgaacgcc ttccggtgtg ttttccggaa gccggagtcc ggatggatca aagaatcaga
     1141 tgattgaaaa gatcagagaa tcagatgatc agatggaagg agaacgaagc atgaattttt
     1201 tggagattga aaaggtaatc ggaagagaga tactggattc cagaggcaat cctaccgttg
     1261 aggcggaggt cacacttgcg gatggtaccg tgggaagagg catggcaccg tcaggcgctt
     1321 cgacgggaga gtttgaggca ctggagcttc gtgatggaga taaagcgaga taccttggaa
     1381 agggtgtcac aaaagcagtg gagaatatca acacggtcat taacgatacg atctgcggca
     1441 tggatgcaag cgatatttac gcgctggatg cggcaatgat tgcagtggat ggaacaaagg
     1501 acaagtcaaa gcttggagca aatgccatcc ttgccgtatc gattgcggcg gcaagagcgg
     1561 cggcgatcgc gcaggatatc ccgctgtacc gtttccttgg cggaatttcc ggaaacagac
     1621 ttccggttcc gatgatgaac atcttaaacg gcggcgcaca tgcagccaac accgtggatg
     1681 tgcaggagtt tatgatcatg ccggtcggtg cgggaagctt taaggaggcg ctccgctggt
     1741 gcgcagaggt atttcatgca ctggcagcgc tgttaaagag caaagggctt gcaacttccg
     1801 tcggcgatga gggtggtttt gcaccggatc tcgccagcga tgaggaagcg atcgagtaca
     1861 ttttacaggc agtgaaaaat gcgggctatg agccgggcaa agatttcatg attgccatgg
     1921 atgcggcgtc ctccgagtgg aaaggcacaa agaagggtga gtacatattg ccgaaggcag
     1981 gtacaaagtt tacttccgag gaactgatcg agcactggaa gaagctggtg gagaagtatc
     2041 cgatcatttc catcgaggat gcgctggatg aggaggactg ggaaggatgg cagaagctga
     2101 cgaaagagct gggcgataag gtacagcttg tcggcgacga tctttttgta acgaacaccg
     2161 agcgtctcaa gaagggaatt gacagtggct gcggaaatgc aatcctgatt aaattaaatc
     2221 agatcggctc tgtgtcagag acactggagg caatcaagat ggcacataag gcaggctata
     2281 cggcaatttc ctcccaccgc tccggcgaga cggaggacac gacgatcgca gatcttgcgg
     2341 tggcattaaa cacctgccag atcaagaccg gcgcaccgag cagaaccgag cgtgtggcaa
     2401 aatacaacca gcttttgcgc atcgaggagg agctgggcga tgctgcggta tatcccggca
     2461 tggcggcctt caatgtgaag cgctgatctt agggagtgag cccggatcgg gatagcggat
     2521 cgtttttgga cttggcatgg cggcagtgca gactgcagat gagcaggagg agactgcttg
     2581 agcagcttat caggacttgg catggcggca gtgcagacag ccacagtgca gaaagaaaag
     2641 aagtctgcag gaaaaataaa actttgaaat gggaaatgta cggacagccg gcgcagaatg
     2701 caccggctgt tttttctgcc tgccaggtgc aaatttttta cagtctatcg gatgcaattt
     2761 ttttacagtt tgatagataa cctgtttttg acgtttttcg ggtcaatgga agtatggagg
     2821 agcttgtcag tc
//
LOCUS       DBMDQJ010000302         2831 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12647_length_2831_cov_1.327106, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000302 DBMDQJ010000000
VERSION     DBMDQJ010000302.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12647_length_2831_cov_1.327106"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            185..1516
                     /gene="aspS"
                     /locus_tag="ACZO9R_06970"
     CDS             185..1516
                     /gene="aspS"
                     /locus_tag="ACZO9R_06970"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HIR0912842.1"
                     /translation="MEYVTGVSKKETVEISEILSGDFEGKQVKVNGAVHTIRDMGEVA
                     FVILRKREGLLQCVFEEGKTKFDLKELKEASTIEVEGTVRAEHRAPNGFEVRLDTIRV
                     LSEPAEQLPFAISKWKLPTTLETNLDHRAIALRNVRERAKFRIQEGVVRGFRDFLYSE
                     GFTEIHTPKIGAKSAEGGANLFRLEYFHRPAVLQQSPQFYKQMMVGVFDREFETAPVF
                     RAEKHNTKRHLNEYTSLDFEMGYIDGFEDIMAMETGFLQYTMKLLERDYAKELKMLGV
                     TLPNVEQIPAVRFDEAKQKVAEKYHRQIRNPYDLEPEEEALIGQYYKEECGADFVFVT
                     HYPSKKRPFYAMDDPADPTYTLSFDLLYHGLEITTGGQRIHDYQMLLDKIEKRGMTTE
                     GMEQYLAVFKYGMPPHGGLGIGLERLTMKLVGEDNVRETTLFPRDLSRLEP"
     gene            1698..2291
                     /locus_tag="ACZO9R_06975"
     CDS             1698..2291
                     /locus_tag="ACZO9R_06975"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR0912843.1"
                     /translation="MNIEQQIADYKPYNEQEAHDQAVMRQLLATQPDLFTRENQVAHM
                     TASSWLLNATHDKVLMIYHNIYHSWAWTGGHADGDRDLLAVAKREAMEETGVTEIRAI
                     SEDIFSLEILTVDGHEKRGAYVPSHLHLNVSYLLEADEAEVLRIKPDENSGVRWFFLE
                     EALAACSEPWMIERVYKKLNAKVRERFGGTYGMQRGE"
     gene            2288..>2831
                     /locus_tag="ACZO9R_06980"
     CDS             2288..>2831
                     /locus_tag="ACZO9R_06980"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009214384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0912844.1"
                     /translation="MTVKDLGTKRLETERLILRRFTLEDAEKMYENWAGDPEVTKFLT
                     WPTHESAEISRQILKEWVAAYDNPEVYQWCIEEKESGEPVGSIAVVSRNEKAQSCEIG
                     YCISRSRWHKGITTEALAEVMRYLFEEVGVLRLESRHDPKNPYSGEVMKKCGMHYEGT
                     RIRADWNNTGICDCALYGMVT"
BASE COUNT          812 a          595 c          849 g          575 t
ORIGIN      
        1 gaatacaagc agaagttgca gaagaagatc ccgccccgga aagacagttt gtctttccgg
       61 ggcttttttg ttctatttag gaaatattgc atcaggttaa atgaaaaagc agccgatgca
      121 cgaaaggaat cccgtcccgc acctagatca atccggcagc accggacgaa aaggagcgta
      181 caatatggaa tatgtaacag gagtgagcaa aaaagaaacg gtggaaatca gtgaaattct
      241 ttccggggat ttcgagggaa aacaggtaaa agtgaatggc gctgtccaca ccatccgtga
      301 tatgggtgag gtcgcatttg tgatcctgcg caagagagag ggattactgc agtgcgtatt
      361 tgaagaggga aaaacaaagt tcgatttaaa ggaactgaag gaagcgtcga ccatcgaggt
      421 ggagggaacc gtgcgggcag agcaccgcgc accgaacgga tttgaggtac gtctggatac
      481 aatccgcgtg ttgtcggaac cggcggaaca gcttccgttt gcgatttcca agtggaaact
      541 tccaacgacg ttggagacga atctggatca ccgtgcgatt gcgttgcgca acgtcagaga
      601 gcgcgcgaag ttccgcatcc aggagggcgt ggtcagagga ttccgtgatt ttctgtacag
      661 cgaagggttt acggagattc atacaccgaa gatcggagcg aaaagcgccg aaggcggcgc
      721 gaatttgttc cggctggagt attttcacag accggcagtg ctgcagcaga gcccgcagtt
      781 ttacaagcag atgatggtgg gcgtgttcga ccgggaattt gagaccgcgc cggtgttccg
      841 tgcggaaaag cacaatacca agcgtcattt gaacgaatat accagccttg actttgagat
      901 gggatacatc gacggatttg aggacatcat ggcgatggaa accggatttt tacaatatac
      961 catgaagctt ttggagcggg attacgccaa ggaattaaag atgcttggcg tgacgcttcc
     1021 gaatgtggag cagatcccag cggttcgctt tgatgaggcg aaacagaaag tggcggaaaa
     1081 atatcaccgc cagattagaa atccttacga tcttgagccg gaagaggaag cgctgatcgg
     1141 acagtattat aaggaagaat gtggggcgga ttttgtattt gtgacacatt acccgtcgaa
     1201 aaaacgtccg ttttatgcga tggatgatcc cgcagatccg acatacacgc tgagctttga
     1261 cctgttgtat cacggactgg agatcacgac aggtggtcag agaatccacg attatcagat
     1321 gctgctggat aagatcgaga agcgcggcat gacgaccgag ggcatggaac agtatctggc
     1381 ggtattcaaa tacggcatgc caccgcacgg cggactggga atcggacttg agcgcctgac
     1441 catgaagctg gtcggcgagg acaatgtccg cgagacaacc ctgttcccaa gggatttaag
     1501 ccgcctggaa ccgtaaacaa gacccaagag aacaaagcgg cttaaatcgc ttttggatct
     1561 aagccggtta gaaccgtaga aaaataaaaa gcaacaaagc ggcttaaatc ccttttagat
     1621 tctaagccgc ctggaactgt agaaaaatgc aaagagaaca aaacggctta aaataacaac
     1681 agaaaaggaa ctcacacatg aacatagaac aacagattgc agactacaag ccgtacaatg
     1741 agcaggaagc acacgatcag gcagtgatgc ggcagcttct tgccacacag ccggatttat
     1801 ttacaagaga gaatcaggtg gcgcatatga cagcgtcatc gtggctgttg aacgccacgc
     1861 acgataaggt gctgatgatc tatcataaca tttatcactc gtgggcgtgg acgggcggtc
     1921 atgcggacgg agacagagac cttctggcgg ttgcgaagcg cgaggcgatg gaggagaccg
     1981 gcgtgacaga gatacgggcg atttcggaag acatcttttc acttgagatt ctgacggtcg
     2041 acgggcatga gaagcgtgga gcctacgtgc cgtcgcatct gcatctgaat gtgtcgtatc
     2101 ttctggaggc ggatgaagcc gaagtgcttc gcatcaagcc ggacgagaac agcggtgtgc
     2161 ggtggttttt cctggaggag gcgctcgcgg cgtgcagtga accgtggatg attgagagag
     2221 tatacaaaaa gctgaatgca aaagtgcggg agcgtttcgg cgggacgtac gggatgcaga
     2281 gaggagaatg acagtgaaag atttgggaac aaaacggctt gagacagagc ggctgatttt
     2341 gagacgtttt acattagaag atgcagaaaa aatgtatgag aactgggcgg gcgatcctga
     2401 ggtcacaaag tttctgacct ggccgacaca cgagagcgca gagatcagca gacagatatt
     2461 aaaagagtgg gtagcggcct atgacaatcc ggaggtatac cagtggtgta tcgaggaaaa
     2521 ggagagcgga gagccggtcg gcagtatcgc tgtcgtgagc cgcaatgaaa aggcacagtc
     2581 ctgcgagatc ggttattgca tcagcaggag ccgctggcac aaaggcatta ccacggaggc
     2641 acttgccgag gtcatgcgct atctgtttga ggaggtcggc gtactgcggc tggagtccag
     2701 acatgatccg aagaatccat attccggaga ggtcatgaaa aaatgcggca tgcactacga
     2761 gggtactaga attcgtgcag actggaacaa taccggtatc tgtgattgcg cattgtacgg
     2821 catggtgaca t
//
LOCUS       DBMDQJ010000303         2819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12718_length_2819_cov_1.625092, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000303 DBMDQJ010000000
VERSION     DBMDQJ010000303.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12718_length_2819_cov_1.625092"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="ACZO9R_06985"
     CDS             complement(<1..605)
                     /locus_tag="ACZO9R_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912845.1"
                     /translation="MFSLKAPKNAAAAPATSTSRLDLHPVAHIADCLLDYQKQLSTSE
                     VHSLDELQSITQAFQLVLDENAALREKLDSFHELFGDVDRASGEFASVKSDIASSVGL
                     AQQRVDGLKDSSKEVQERFLEMQNTFSDFQVSVQKIKDCMAQIISIANQTNMLALNAS
                     IEAARAGEQGKGFAVVAEEVKNLASEIKSLVSTVDISIGDV"
     gene            complement(632..1687)
                     /locus_tag="ACZO9R_06990"
     CDS             complement(632..1687)
                     /locus_tag="ACZO9R_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004082085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FIST signal transduction protein"
                     /protein_id="HIR0912846.1"
                     /translation="MKQYFGMSQTGNLKEAAQGVHAPGLLILMSNADQFEAHVTELES
                     LFPGVPSIGCIGMSYSTRIVEKGVGLVAFYDNVTAVANVLEQASTMPVKYIERMEQDL
                     KNVHASSKDTVCIDLCTGNDACVLTTIHSILGKAGIPLVGGTGDGGKVSVNGTVYEDA
                     DVYAFVKNNGGKVAVYKENLYKPMPGYRFIASNTDRSRYYVGELNGRSSKQAYMDALH
                     ISESNIINQTFHNPLGKMNGQDICIISLKEVSGNGLCCYRQVNDSDVLVLLEEQDYNE
                     ILDETLQRIRRDFNHISGVFSINCVFRYLLFSQEHHMEDYLKTMSTLGDHAGLIGFGE
                     HYNQQFVNQTMTCVVFE"
     gene            1895..2128
                     /gene="acpP"
                     /locus_tag="ACZO9R_06995"
     CDS             1895..2128
                     /gene="acpP"
                     /locus_tag="ACZO9R_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HIR0912847.1"
                     /translation="MLEKMRPIIAEQLNVQESEIKENTKLKEDLGADSLDLFELVMAL
                     EEEFGVEIPSEDLESIVTVGDVITYLKNRGVTA"
     gene            2203..2727
                     /locus_tag="ACZO9R_07000"
                     /pseudo
     CDS             2203..2727
                     /locus_tag="ACZO9R_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
BASE COUNT          727 a          646 c          771 g          675 t
ORIGIN      
        1 tcgacgtcgc cgatgctgat atctaccgtg ctgacaagac tcttgatctc actcgcaaga
       61 tttttgacct cctcggcaac gaccgcaaag ccttttccct gctctccggc gcgggcagcc
      121 tcaatcgaag cattcagggc aagcatgttt gtctggttgg caattgagat gatctgtgcc
      181 atgcagtcct tgatcttctg aacggaaacc tggaaatcgg aaaaggtgtt ctgcatttcc
      241 aaaaagcgct cctgcacttc tttggagctg tctttcaatc cgtcaacacg ctgctgtgca
      301 agaccgacag agctggcaat gtcagatttg acggaagcaa attcaccgga agccctgtcc
      361 acatcgccga acagctcatg aaaggaatcc agcttttcgc ggagtgcggc attttcgtcc
      421 agaacgagct ggaatgcctg tgtgatggac tgcagttcat ctaaagagtg cacttcgctg
      481 gtggagagct gcttctggta atctaacagg cagtccgcaa tatgcgcaac gggatgaaga
      541 tctaagcggc tggtggacgt ggctggtgca gccgctgcat tctttggtgc ttttaatgaa
      601 aacatggaaa tcctcctctt tctatcacag attattcaaa tacgacacag gtcatggttt
      661 ggttgacgaa ctgctgatta taatgttctc cgaatccaat cagaccggcg tgatcaccga
      721 gtgtggacat ggttttcaga taatcttcca tatggtgttc ctgtgagaac agaaggtaac
      781 ggaaaacaca gttgatggag aaaacaccgg aaatatggtt gaaatcgcgg cggatgcgct
      841 gcagcgtctc atccagaatc tcgttgtaat cctgttcttc cagaagaacg agcacatcgg
      901 aatcattgac ctgtctgtaa cagcagagac cattcccgga tacttccttt aaggaaataa
      961 tacagatgtc ctgtccgttc attttgccga gcggattgtg gaaggtctgg ttgatgatat
     1021 tgctctcgct gatatgtaag gcatccatat aagcctgctt ggaagatctg ccgtttaact
     1081 cgccgacata gtatctgctg cggtcggtat tggaagcgat gaagcggtag cctggcatcg
     1141 gtttgtagag attctccttg tagacagcaa cttttcctcc gttgtttttc acaaaagcgt
     1201 agacatcggc gtcttcatag accgttccgt tgacagagac ttttccaccg tccccggtgc
     1261 cgccgacaag cggaattcca gccttgccga gaatgctgtg gatcgtggtc agcacacagg
     1321 cgtcatttcc ggtacacagg tcaatacaga cggtatcttt cgaacttgcg tggacgtttt
     1381 ttaaatcctg ttccatgcgt tcgatgtatt tgaccggcat ggtggatgcc tgctccaaaa
     1441 cgttggcaac agcggtgaca ttgtcataaa aagcaaccag accgactccc ttttcgacga
     1501 tcctggtact gtaactcatg ccgatacagc caatgctcgg gactcccggg aaaagggact
     1561 ccagttctgt aacgtgtgcc tcaaactggt ctgcgttgga catcaggata agaagtcccg
     1621 gagcgtgaac gccctgtgca gcttctttta agtttcctgt ctggctcatt ccaaaatatt
     1681 gtttcatcag cgaactcctc ccacatctta ttacaaatat tgtaagcaat tttgcatgat
     1741 atttcaacgc attaatgcaa ttttagcaaa acgtttccaa aaaatacctt gacaatcaaa
     1801 caggacagac atatactttg aaaagcaaat aatttgatta tcaaaatatt tgaaaagcaa
     1861 ataattaaaa ggaacaggga aaaggagaag aaacatgtta gagaagatga gaccgatcat
     1921 tgcggagcag ttaaacgtac aggagtctga gattaaagag aacacaaagt taaaggaaga
     1981 tctgggtgcg gattcgctgg atctgtttga acttgtcatg gcgctggagg aggaattcgg
     2041 cgtggagatt ccgtcggagg atctggaatc gatcgtgacg gtcggagatg tgatcacata
     2101 tttgaagaac cgcggggtta cagcataggc gaagacagca gccgggacat accggtgttt
     2161 aagggtaaga aaacagaccg gcaggaagaa aagaggagaa gcatggaaac aagggtaagc
     2221 agattgcttg gaacaaaata tccggtcatt cagggtggta tggcatgggt ggcggaatat
     2281 cacctggcgg cagcggtgtc gaacgcgggg ggactcggaa tcatcggggc ggcaagcgcg
     2341 ccgccggaag tggtaagaga acagatccgt gaggtaaagg cgcggacgga ccgtccgttt
     2401 ggcgtgaacg tcatgctgct taatccaaac gcgccggaag tggcgaagat tgtcatagaa
     2461 gagaaagtgc cggttgtcac aaccggtgcg ggaagtccgg caaagttcat ggaggcatgg
     2521 aagcaggcgg gcgtggtggt catcccggtc gtggcgagcg ttgccatggc gaagatgatg
     2581 gagcgtgctg gcgcggacgc tgtgattgca gagggaatgg agtccggcgg acatatcggt
     2641 gcgcagacga cgatgacgct ggtgccgcag gtggcggacg ccgtgtcggt tccggtcatt
     2701 gcagcgggcg gtatcgcgga cggaagaccc tcgctccggc tgtggaaatg atggaaaacc
     2761 ctatcgggtt taccacatta ccacagcctc cgctcatccc ccttttcccc tccgacttt
//
LOCUS       DBMDQJ010000304         2819 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12721_length_2819_cov_1.261957, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000304 DBMDQJ010000000
VERSION     DBMDQJ010000304.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12721_length_2819_cov_1.261957"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(464..1495)
                     /locus_tag="ACZO9R_07005"
     CDS             complement(464..1495)
                     /locus_tag="ACZO9R_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5050 domain-containing protein"
                     /protein_id="HIR0912848.1"
                     /translation="MKKKISIIIAVIVIIALIAAAAIFHYLSNRTHFNEGYVNGNTAG
                     NLYNGGLFCEYDGTVYFSNPSDGGKLYSMSPDGSNLAKLCDDTVSYINADEHYLYYVR
                     NNPGATGAALSFLSVNTDSLCRIDREGGDDSVLILDSAPCLYASLYGNYIYYIRYDES
                     EGSTLYKVKIDGSGCKQVDTAPDFTCSANGEYMYYNGTDSDHYIWRLNTTDDSKGMLY
                     GGNCYMPTVIDDTTAYFMDCDSNYAIARTDLATGEKTLLSEDRVDWYNLYGDYIYFQK
                     NDSEHPCLGFMRTDGSGYTTLFEGNFKDITITSEYVYFRDFSTDQSYCVPLGSSASPV
                     AFEPGTVED"
     gene            1694..>2819
                     /gene="pheA"
                     /locus_tag="ACZO9R_07010"
     CDS             1694..>2819
                     /gene="pheA"
                     /locus_tag="ACZO9R_07010"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HIR0912849.1"
                     /translation="MKNLLELRDEIDVIDKQIVALYQQRMQIAAEVAEYKIETGKKVF
                     DKDREMEKLATLSALGDSAFNRHGIRELFEQIMSISRKRQYQLMTEHGIYEKPDFEEL
                     DALDYKNARIVFQGTEGAYTQLALKQYFGEDAGNSYHVETWRDAMEAIASGDADYAVL
                     PIENSSAGIVSENYDLMVEYGHCIVGEQIIKIEHALLGVPGAKLSDITDVYSHPQALM
                     QCARYLEGHREWEKHSLKNTAMAAQKVREDGMRHKAAIASRLTAEIYGLDVLEEGIQD
                     NKQNATRFIIVTGKHVFTRKANKISICFEGAHETGSLYHMLSHLIYNGINMDHIESRP
                     LPERNWEYRFFVDFEGNLNDPAVQNALRGLSEETTRLQILG"
BASE COUNT          723 a          620 c          807 g          669 t
ORIGIN      
        1 catctgtgat gatccatgtc tttccaagtt tggagcactc cacggattgt gtccgcaagc
       61 cagcagacaa ttttaggact tttgtgtgtg tcccttatgg cacctctgag caaaacataa
      121 ccgcgcgaag ccgctgtctg agtcggcatc tgccctattt tcaatagcag atgtcggcga
      181 gttcggttcg cgcggtttta tattgtctgt gaagaggtgc cattaggaac accagaaagt
      241 ccgctcattg tctgcgactt gtcggacaca atccgtgagt gtccaaacgt ggccaagaca
      301 cggaatggga tgccagccgc ggcatggaag aaatactcga attttgcttc aaagaatttg
      361 ccgccgtctg tttattctgg aacttttttg gtctctggta ttcctgtaat attacttatc
      421 gctcgaaaga ggtctatgct tattgcgaca attccttatg gtatcagtct tcgacggtac
      481 ccggctcaaa ggcgaccggg gaggcggagg agccgagcgg aacgcagtag ctctgatctg
      541 tggaaaaatc acggaagtat acatattcgg aagtgattgt aatgtccttg aaatttccct
      601 cgaacagtgt ggtgtaaccg ctgccgtccg tgcgcatgaa tccgagacac ggatgttcgg
      661 aatcgttttt ctggaagtag atataatccc cgtagaggtt gtaccagtcc acgcggtcct
      721 cggacagaag tgtcttctca ccggtggcaa gatccgtgcg ggcgatcgcg tagttgctgt
      781 cgcagtccat gaaataggcg gtggtatcgt ctatgacagt cggcatataa cagttcccgc
      841 cgtagagcat gccctttgag tcgtccgtgg tattgagccg ccagatatag tgatcgctgt
      901 cggttccgtt gtaatacata tattcgccgt ttgccgagca ggtaaagtcg ggagcggtgt
      961 cgacctgctt gcagccagaa ccgtcgattt ttaccttata aagtgtggaa ccctcggatt
     1021 catcatagcg gatatagtaa atataattcc cgtagaggga agcgtagagg cacggcgccg
     1081 aatccagaat cagaacggag tcatcgccgc cttccctgtc aatgcggcag agactgtcgg
     1141 tgttgacaga gagaaaggag agagcggcgc ccgttgctcc cggattgttg cgcacatagt
     1201 agagataatg ttcgtccgcg ttgatgtagg ataccgtgtc atcacagagc ttggccagat
     1261 tgcttccgtc cggagacatc gaatacagct tcccgccgtc ggacgggttg gaaaaatata
     1321 ccgtgccgtc gtactcgcag aagagaccgc cgttgtagag gttgccagcg gtgttgccgt
     1381 tcacgtaacc ctcattaaaa tgagtccggt tggagagata gtggaaaatg gcagccgctg
     1441 cgataagcgc gataatgacg atgacagcaa tgataatgga aatctttttt ttcatggaat
     1501 gattccttct ttcgtctgaa atgccgataa aatataatca tggaaattat aacattttcg
     1561 tctaataact tgcaagggtt ctcttgtcat cagcacgtta atgtactata ttataaacta
     1621 aggcggagtc ggcacaaaag agcacccggc ggatgccctg atacgaaaag gaagtcagac
     1681 ggtaaggagc actatgaaga atctattaga gttgagagat gagattgatg tcattgacaa
     1741 gcagatcgtt gcgctgtatc agcagcggat gcagatcgcg gctgaggtgg cggaatataa
     1801 gatagagacg ggcaaaaaag tttttgacaa agaccgtgaa atggaaaagc ttgcaacgct
     1861 ttcggcgctc ggcgacagcg cgttcaaccg gcacggaatc cgggagctgt ttgagcagat
     1921 catgtcgatc agcagaaagc gccagtatca gctgatgacg gagcacggca tatatgaaaa
     1981 gccggatttt gaagagctgg atgcgctgga ttataaaaat gcgcgtattg tctttcaggg
     2041 gacggagggc gcctacacgc agctcgcgtt aaagcagtat ttcggtgagg atgcggggaa
     2101 cagctatcat gtggagacct ggagggacgc catggaggcg atcgccagcg gggacgcgga
     2161 ttatgcggtg ctgccgattg agaattcatc tgcagggatt gtctccgaga attatgatct
     2221 catggtggag tatggacact gcatcgtggg cgagcagatt atcaagatcg aacatgcctt
     2281 gcttggcgtg ccgggagcga agctttccga tatcacggat gtatactcgc atccgcaggc
     2341 actgatgcag tgtgcgagat acttagaagg gcatcgggag tgggagaagc acagcttaaa
     2401 aaataccgcg atggcggcac agaaagtcag ggaggacggt atgcggcaca aagctgccat
     2461 tgcaagccgt cttacggcgg agatctacgg tcttgatgta ctggaggaag gcattcagga
     2521 caacaagcag aatgcaacca gattcatcat cgtgacggga aaacatgtgt ttaccagaaa
     2581 ggcgaataag atcagtatct gctttgaggg agcgcatgag accggttctc tctaccacat
     2641 gctgtcccat ctgatctaca acgggatcaa catggatcac atcgagtcca gaccgctgcc
     2701 ggagcgcaac tgggaatacc ggttctttgt agattttgag gggaatctga acgatccggc
     2761 ggtgcagaat gcactgcggg gattgtcgga agagacgacc agactgcaga ttctgggga
//
LOCUS       DBMDQJ010000305         2799 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12825_length_2799_cov_1.766864, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000305 DBMDQJ010000000
VERSION     DBMDQJ010000305.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12825_length_2799_cov_1.766864"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="ACZO9R_07015"
     CDS             <1..287
                     /locus_tag="ACZO9R_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015530507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HIR0912850.1"
                     /translation="TGIFAGGVLFGTAGIKVLSSKDAKKVYTSCTAAFLRAKECVMNT
                     VSTVQENAGDIYAGAKEINEERAAKAEAEAFADAEAEAAEKTGTAENTEE"
     gene            311..2386
                     /locus_tag="ACZO9R_07020"
     CDS             311..2386
                     /locus_tag="ACZO9R_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR0912851.1"
                     /translation="MKFAIKHEIKGRMRIHVAQYRMSCAQADTLLYFLQNDIQVSSAK
                     VYERTGDAVICYDGSRAELIDKLRHFHYEDVEVPNGLIENSGRELNASYQEKLIGRVV
                     RRYASKIFLPYPIRACWTTVKSFRYIWKGIKTLAARKIEVPVLDATAIGVSILRGDTE
                     TAGSIMFLLGIGELLEEWTHKKSVGDLARTMSLNVGKVWLKKDGVEVLVGAKEVREGD
                     EVVVHMGNVIPFDGVVTDGEAMVNQASLTGESEPVRRISENYVYAGTVVEEGELTILV
                     KAVGGSSRYDKVVQMIEESQKLKSGLESRAEHLADRLVPYSLGGTALTYLLTRNVTKA
                     LSILMVDFSCALKLAMPISVLSAIREASFYNVTVKGGKYLEAMAEADTIVFDKTGTLT
                     KAKPTVAKVVSFNGDSPDELLRIAACMEEHFPHSMAKAVVEAAREKGLDHEEMHSKVE
                     YVVAHGISTTINGRKAVIGSYHFVFEDEGAALPEGTKELFESLPEEYSHLYLAIEGQL
                     AAVICIEDPLREEAEAVINSLRRSGIKKIVMMTGDSERTASAIAKRVGVDEYYSEVLP
                     EDKAGFVEREKAAGRKVIMVGDGINDSPALSAADVGIAISDGAEIAREIADIMVSSDD
                     LYQIVTLKLLSDGLMKRIRKNYRVIVGFNTGLILLGVGGVLQPTTSALLHNTSTLVIS
                     LKSMQNLLS"
BASE COUNT          743 a          591 c          845 g          620 t
ORIGIN      
        1 agacaggaat ttttgcaggc ggcgtattat ttggaacagc cggtatcaag gtactttcca
       61 gcaaggatgc caagaaggtt tacacaagct gtaccgcagc tttcttaaga gcaaaagagt
      121 gtgtgatgaa caccgtaagc accgtacagg agaatgccgg agacatctat gcaggcgcaa
      181 aggagatcaa tgaggagcgc gcagccaagg ctgaggcaga ggcatttgca gatgcagagg
      241 cagaagcagc agagaagacc gggactgcag agaacaccga agaataagat tttgataaga
      301 ggcgagtaaa gtgaagtttg caatcaagca tgagattaag gggcggatgc ggattcacgt
      361 ggcacagtac cgcatgagtt gtgcacaggc ggatacgctt ctgtattttt tacaaaatga
      421 tatacaggtc tcaagcgcca aggtgtatga gcgtacgggt gacgcagtca tctgttatga
      481 cggcagcaga gcggaactga ttgacaagct cagacatttc cactatgaag atgtggaggt
      541 gccgaacggg ctgatcgaga attccggaag agagctgaat gcaagctacc aggagaagct
      601 gatcggcaga gtggtgagac gctatgcatc caagattttt ctgccgtatc ccatccgggc
      661 gtgctggacg acggtcaagt cgttccgcta catctggaag ggcataaaga cactggcggc
      721 acgaaagatc gaagtaccgg tactcgatgc gacggcgatc ggagtgtcta ttctgcgcgg
      781 tgataccgag acggcgggat cgatcatgtt cctgcttggc atcggcgagc tgttagaaga
      841 gtggacgcac aagaagtccg tcggcgacct tgcgcgcacc atgtctttga atgtcggcaa
      901 agtatggctg aagaaggacg gcgtggaggt tcttgtcggc gcgaaggaag tccgtgaggg
      961 agacgaggtg gttgtccata tgggcaatgt aatcccgttt gacggcgtcg tgacagacgg
     1021 cgaggcgatg gtcaaccagg catccttaac cggagaatcc gagccggtac gccggattag
     1081 tgagaattat gtctatgcag gaactgtagt cgaggaaggg gagcttacca ttctcgtgaa
     1141 ggcggtaggc ggctccagcc gctacgacaa ggttgtgcag atgatcgagg aatcccagaa
     1201 gttaaagtcc gggcttgaga gccgggcaga gcatctggcg gaccgtctgg taccgtattc
     1261 cctcggtggc acggcgctca cataccttct gacccggaat gtgacgaagg cgttgtcgat
     1321 tctgatggtg gatttctcct gcgcattaaa acttgccatg ccgatctccg tattgtcggc
     1381 gatccgtgag gctagttttt acaatgtgac ggtcaagggc ggaaaatatt tagaggcaat
     1441 ggctgaggca gatacaatcg tctttgataa gaccggcaca ctgaccaagg caaagccgac
     1501 cgtggcaaaa gtggtttcct ttaacgggga cagcccggat gaacttctta ggattgcagc
     1561 ctgcatggag gagcatttcc cacattccat ggcaaaagcg gttgttgagg cagccaggga
     1621 aaaaggtctg gatcatgagg aaatgcattc caaggtggag tatgtggtgg cacatggtat
     1681 ttccaccacg atcaacggca ggaaagccgt gattggaagc tatcattttg tatttgagga
     1741 tgagggagct gcccttcctg agggtacgaa ggaactgttc gagagcctgc cggaggagta
     1801 ttcccatctg tatctggcca tcgagggaca gcttgcagcc gtgatctgta ttgaagatcc
     1861 gctccgtgag gaggcggagg cagttatcaa ttccctgcgc cgctccggta tcaagaagat
     1921 cgtcatgatg acgggtgaca gcgagcggac ggcaagcgcc atcgcaaagc gcgtcggtgt
     1981 ggacgagtat tattccgagg tgcttccgga ggataaagcc ggttttgtcg agcgtgaaaa
     2041 ggcagccgga cgcaaggtta tcatggtcgg agacggcatc aacgattccc cggcactctc
     2101 tgcggcggat gtcggcatcg cgatttcgga cggtgcggag attgcgcgtg agatcgcgga
     2161 tatcatggta agctcggatg atctctatca gatcgtgacc ttaaagctgt tgagcgacgg
     2221 cctgatgaag cggattcgca agaattaccg cgtgattgtc ggattcaata ccggattgat
     2281 cctgctagga gtcggcggcg ttttacagcc gaccacatca gcgcttctgc ataacacctc
     2341 tacgctggtc atcagcttaa agagcatgca gaatcttttg agctaactga atatgtattt
     2401 tgaaaagtct ttacagattt gccactggca gtaaaatgcg gcagaactgt gaaggctttt
     2461 ttatttcata ggaaataccg tgatacattc atgtgtgaaa gtggaacttt cctatgaaag
     2521 tttcagagaa aacattctga tacatttcca aaaatttaca aatttattga tgacaaaata
     2581 aaaaatgtgt ataataaagg cagatgtaaa aagttggcag ataatagcat acagaaagag
     2641 agtaccggat atgagaaaaa aatggatcag actgattgcg gcagccatgg gaagttttct
     2701 gcttgccggc tgcggtgcag atacgcagat tgatgattat gctacaaatg ccggagcgga
     2761 gagcgtgcag cagacgtcag ggacggagtc ggcggctgc
//
LOCUS       DBMDQJ010000306         2799 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12826_length_2799_cov_1.537806, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000306 DBMDQJ010000000
VERSION     DBMDQJ010000306.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12826_length_2799_cov_1.537806"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="pncB"
                     /locus_tag="ACZO9R_07025"
     CDS             complement(<1..837)
                     /gene="pncB"
                     /locus_tag="ACZO9R_07025"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR0912852.1"
                     /translation="MSTLNLTLLTDLYELTMMQGYFKNHNQDVVVFDAFYRNNPCDGG
                     YSVCAGLAQVIDYIENLHFDADDINYLRSLKIFDEDFLEYLSNFKFSGDIYAVPEGSL
                     IFPREPLVKVVAPIMEAQLIETAILNIVNHQCLIATKAARVCYAAKGDNVMEFGLRRA
                     QGPDSGTYGARAAVIGGCNGTSNVLAGQMFHVPVLGTHAHSWIMSFPDEYTAFRKYAE
                     LYPSACILLVDTYDTLKSGIPNAIRVFTEMRDAGIPLTYYGIRMDSGDLAYLSKQVRK
                     MLD"
     gene            969..1451
                     /locus_tag="ACZO9R_07030"
     CDS             969..1451
                     /locus_tag="ACZO9R_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HIR0912853.1"
                     /translation="MRRERAMALFEEGYNCAQSVFLAFSDLHGMDTHTAAVLSSSFGG
                     GMGRLREVCGAVSGMFLTAGILYGYDDPGAREEKAVHYARIQELAKAFEEKNGSIVCR
                     ELLGLSVKREEPTPKERTAEYYKKRPCKELVGDAAEILEKYIAEHPVDERLEKRGTVR
                     "
     gene            1448..2584
                     /locus_tag="ACZO9R_07035"
     CDS             1448..2584
                     /locus_tag="ACZO9R_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912854.1"
                     /translation="MMIRRAAIQDLEQIMQIYARAVQYMHASGNPNQWVTGYPSRTLI
                     EEDIARGVSYVVDRDSVIEAVFSYIEGEDPTYRQIGQGMWHTNGSYGTVHRLASAGRL
                     RGVAGICFSWCAEQAKMHGCSSLRADTHQDNRIMQHLLQKNGFVYCGVIHLANGAPRL
                     AFERAAVAPEGAYAGMQNPNGMNPGGAYAGTPNANGMNPGMPQQKQKGDGRGYGIASL
                     ILGIVSVLLFCTCINWVTGILAIIFGIIQITKNKEQGLAIGGIITAAISMVLSVVLYL
                     AMWFGMTNAGITYEDLLYDQYDDSYDSDGSDAWYGGGGNGSYDGGGSDGWYDDGGNDG
                     WYDDGGNDGWYDGDGSGGYDYYNDPYDYFRDYYDVQEPGGNQFM"
BASE COUNT          714 a          580 c          874 g          631 t
ORIGIN      
        1 gtccaacatt ttgcgcacct gcttggagag gtaggcgagg tcgccgctgt ccatgcggat
       61 tccgtaataa gtcagcggga taccggcatc gcgcatctcg gtgaagacac ggatggcatt
      121 cggaataccg gattttaacg tgtcgtaggt atccacgagc agaatacatg cggacgggta
      181 gagctcggcg tatttgcgga acgcagtgta ctcatccgga aaactcatga tccagctgtg
      241 ggcgtgcgtg ccgagtaccg gcacatggaa catctgaccg gcgagaacat tggaagtgcc
      301 gttgcagccg ccgattacgg cagctctggc accgtaggtg ccggaatccg gaccctgtgc
      361 gcgccgtaaa ccaaactcca tgacattatc gcccttggcc gcgtaacaga cgcgggcggc
      421 cttggtcgcg atcagacact ggtggttgac gatatttaag atggcagtct cgattaactg
      481 tgcctccatg atgggagcga caacttttac cagaggttct cgcgggaaga tcaggctgcc
      541 ttccgggacg gcgtagatgt cgccggaaaa tttgaaattg ctcaggtatt ccagaaaatc
      601 ttcgtcaaat attttcagac tgcgcagata gttgatgtcg tccgcgtcaa aatgcagatt
      661 ctcgatgtag tcgatgacct gcgctaaacc tgcgcagacg gaataaccgc cgtcgcaggg
      721 attgttgcgg tagaaggcgt caaagacgac aacatcctga ttatgatttt taaaatatcc
      781 ctgcatcatg gtcagctcat agagatctgt gagcagggta agattcaatg tactcatgtg
      841 tgtctggaac caaagttccg ctcctttcct gttttgagat tggaactatt ataacataat
      901 cgttgagaaa atgtaggaaa agttatatag ttaaggtatt ctggaagaaa tgtgaggcag
      961 tgaatgggat gagaagagag cgtgcgatgg cactgtttga agaaggatat aattgtgcgc
     1021 agtcggtatt tctggcgttt tcggatttac atggaatgga cacgcacacg gcggcggtac
     1081 tcagctcgtc tttcggcggc ggaatgggaa gactgcgcga ggtgtgcgga gcggtcagcg
     1141 ggatgtttct tacggcgggc atattatacg gctatgacga cccgggtgcc cgggaggaaa
     1201 aagcggtaca ctatgcgaga atccaggaac tggcgaaggc gtttgaggag aaaaacggtt
     1261 cgattgtctg tcgggagctg cttggcttat cggtaaagcg ggaagaaccg acgccgaagg
     1321 aacggactgc cgagtattat aaaaagcgcc cctgcaagga acttgtcggg gacgccgctg
     1381 agatattgga aaagtatatt gcagaacatc cggtggatga gaggttagaa aagagaggaa
     1441 cagtcagatg atgattcgca gagcagccat acaggatctg gaacagatca tgcagatata
     1501 tgcgagggcg gtgcagtata tgcacgcgtc cggcaacccc aaccagtggg tgacggggta
     1561 tccgtccagg acgttgattg aggaggatat cgcacgtgga gtcagttatg tagttgacag
     1621 ggacagtgtg attgaggctg tgttttccta tatagaaggg gaggatccga cctaccggca
     1681 gattgggcag ggaatgtggc acacgaatgg aagttatgga accgtgcacc ggctggcatc
     1741 cgccggacgg ctccgcggcg tggcgggaat ctgcttttcc tggtgtgccg agcaggcaaa
     1801 aatgcatggc tgcagcagtc ttagggctga cacgcatcag gacaaccgca ttatgcagca
     1861 tcttctgcaa aaaaacggat ttgtgtactg cggggtgatt catctggcaa acggcgcgcc
     1921 gcgtctggcg tttgagcggg ctgccgtggc gccggaagga gcatatgcag ggatgcagaa
     1981 tccgaacgga atgaaccctg gaggagcata tgcagggacg ccgaatgcga atggaatgaa
     2041 tccgggaatg ccgcagcaga aacagaaagg ggacggcaga ggatatggca ttgcatcgct
     2101 tatacttgga atcgtgtctg tgctgctgtt ttgtacctgc atcaactggg tgacgggaat
     2161 tctggcgatt attttcggaa tcattcagat cacgaaaaat aaggagcaag gtcttgcaat
     2221 cggtggaatc atcacagcgg cgatctcaat ggtgctttct gtcgttttat atcttgccat
     2281 gtggttcgga atgacaaatg cggggattac atacgaagat ctcctatacg atcagtatga
     2341 tgacagctat gacagcgatg gaagtgatgc atggtacggc ggcggtggaa atggcagcta
     2401 tgacggcggc ggaagcgacg gatggtatga cgacggcgga aatgacggat ggtatgacga
     2461 cggcggaaat gacggatggt atgacggtga tggcagtggc ggctacgatt actataatga
     2521 tccctacgat tatttcagag attattatga cgttcaggag ccgggcggca atcagtttat
     2581 gtgagcactt tctgcagtgg agtgatagct tcgcgctttt cggaatatac tctaagtgac
     2641 agagcataaa ggtcagagta gaggtgcgca tagcaggaag cgggttttat aataaaaaga
     2701 aaagaagcag actgatctgg gtggcagcag gaatggcatg catggcggcg gggtgttgtg
     2761 cggcttttta catgcaggta tccctgcgtg tgccaacag
//
LOCUS       DBMDQJ010000307         2791 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12877_length_2791_cov_1.339405, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000307 DBMDQJ010000000
VERSION     DBMDQJ010000307.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12877_length_2791_cov_1.339405"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="ACZO9R_07040"
     CDS             <1..567
                     /locus_tag="ACZO9R_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HIR0912855.1"
                     /translation="NVINAVKSHDLENMLFSHNGIAGLVFYGFLVLTIVLYMTGHKVP
                     GNIMMVIFLGVPVLLFVFKEPLGNLVTKQHKKMEEGKVMFFVQAFFELFETMLSYFSN
                     TISYVRIGAFAVSHAAMMEVVLMLSGASAGHTNWIVFVLGNVLVCGLEGLVVGIQVLR
                     LEYYEMFSRFYKGTGREFKPFHSQSDRK"
     gene            604..1038
                     /locus_tag="ACZO9R_07045"
     CDS             604..1038
                     /locus_tag="ACZO9R_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005335119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit C"
                     /protein_id="HIR0912856.1"
                     /translation="MSLTVRVLFVVTLLLSIVIPFGYFLIGQRTKKRYKRAIGTNIFF
                     FFSTVLIAGIMLFAGDPVQAAEAAGNTASNATGFGYLAAALSTGLSCVGGGIAVASAA
                     SAALGAISEDSSALGKSLIFVGLAEGVCLYGLIISFMILGKL"
     gene            1040..1348
                     /locus_tag="ACZO9R_07050"
     CDS             1040..1348
                     /locus_tag="ACZO9R_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HIR0912857.1"
                     /translation="MKMYLISDNIDTLTGMRLAGVDGIVVHEREELRCAIENAMEDKD
                     IGVILLTEKFGREFPDLIDEIKLERTMPLLIEIPDRHGTGRKKDFITSYVNEAIGLKL
                     "
     gene            1369..1956
                     /locus_tag="ACZO9R_07055"
     CDS             1369..1956
                     /locus_tag="ACZO9R_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR0912858.1"
                     /translation="MTNEEKITHIRTAAMEEARAEANAIIKQHEDALRSVFEQHQIEA
                     RRQSETRVRAESVTAKQQLNMAMSKAQLELKREMGKIQTELKTELFEEVQLKLLAFMR
                     TEEYKEVLIRYIEKAAQFASGMAMTIYINPSDADKKTYLEERTGMTLTISKVDFIGGV
                     RAVVPEKNVLVDYAFKGALENEYQKFRFRGGVKGE"
     gene            1949..>2791
                     /locus_tag="ACZO9R_07060"
     CDS             1949..>2791
                     /locus_tag="ACZO9R_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR0912859.1"
                     /translation="MSSGKTEKIYGINGPVIYLKGKTDFKMSEMVHVGEEKLVGEVIS
                     LDKDRTTVQVYEETSGLHPGELVEGTGAAVSVTLAPGILNNIFDGIERPLERIADKGG
                     AFITRGVSVDALDRQKLWETHITVAEGDMVQGGTIIAEVPETRAIVHKCMVPPGEEGT
                     VISVVPDGEYTIDETLVTIELFNGEKRELSMTQHWPIRVPRPVSRRFPASVPLVTGQR
                     ILDTMFPIAKGGTAAIPGGFGTGKTMTQHQIAKWSDADIIIYIGCGERGNEMTQVLEE
                     FSELV"
BASE COUNT          768 a          509 c          853 g          661 t
ORIGIN      
        1 aacgtgatca acgcagttaa gagtcatgat ctggaaaata tgttattttc ccacaacgga
       61 atcgcagggc tggtgttcta tggattcctg gtgctgacga tcgtgcttta tatgacggga
      121 cataaggttc cgggtaatat catgatggta attttcctgg gggttccggt gctcttgttc
      181 gtattcaagg aaccgttggg caatctggtg acaaagcagc ataagaagat ggaagaggga
      241 aaggtgatgt tcttcgtgca ggcgttcttc gaactgttcg agacgatgct gagttatttt
      301 tccaatacca tttcttatgt gcgtatcggt gcatttgccg tcagtcatgc ggcgatgatg
      361 gaggttgtgc tgatgttatc cggcgcaagt gcggggcata cgaactggat cgtgttcgtg
      421 cttggaaatg tgctggtgtg cggactggaa gggctggtcg tcggtatcca ggtactgcgt
      481 ctggaatact atgagatgtt cagccgtttc tataaaggaa ccggacggga atttaagccg
      541 ttccacagtc agagtgacag aaagtaataa aattcaaaga tatatttaca ggaggacaaa
      601 aatatgagcc tgacagttag agtattattc gttgtaacat tgttgctgag tattgtaatt
      661 ccatttggat attttctgat cggacagagg acgaagaagc gttataagag ggctatcggc
      721 acaaatatat tctttttctt cagcactgtt ctgatcgcag ggatcatgct ttttgcggga
      781 gatccggtac aggcagcgga agcagccggc aatacggcgt cgaacgcaac aggattcgga
      841 tacctggcag cagcactttc aaccggtctt tcctgcgtcg gcggtggtat cgccgttgca
      901 agtgcagcaa gtgcggctct gggtgcaatt agtgaagatt ccagtgcact tggtaagtcg
      961 ctgatcttcg taggtcttgc agaaggtgtg tgtctgtacg gactgatcat ctccttcatg
     1021 atcttgggta aattgtaaga tgaagatgta tctgatcagt gataatatcg atactctgac
     1081 aggaatgcgt ctcgcagggg tagacggaat cgtggtacat gaaagagaag aactccggtg
     1141 tgcgatcgag aatgccatgg aggataaaga tataggtgtg attctgttga cggagaaatt
     1201 cggaagagaa ttcccggacc tgattgacga gatcaagctg gagcggacaa tgccgcttct
     1261 gatcgagatt cctgacagac acgggaccgg acgtaagaaa gattttatca cgtcgtatgt
     1321 aaatgaagcg atagggttga aattatagac aggaagaagg tggcacattt gacaaacgaa
     1381 gaaaagatca cgcatatccg cacggcagcg atggaagagg caagagcgga ggcgaatgcg
     1441 atcataaagc agcatgagga tgcgctgaga agtgtgttcg agcagcacca gatcgaagcc
     1501 agacggcagt cggagacgag agtgcgtgcg gagagtgtga ctgcaaaaca gcagctgaat
     1561 atggcgatgt caaaggcgca gcttgaatta aaacgtgaga tgggtaagat acagacagaa
     1621 cttaagacag aactgttcga agaagtacag ttaaagcttc tggcatttat gagaacagaa
     1681 gagtataaag aggttctgat ccgttatata gagaaagcag cacagtttgc atccgggatg
     1741 gcgatgacga tctatatcaa tccgtccgat gcagataaga agacatatct ggaggagcgc
     1801 acgggaatga cgctgacgat cagcaaagtg gatttcatcg gcggagtgcg tgcggttgtg
     1861 cctgagaaga atgttctagt ggattatgca ttcaaagggg cactggaaaa tgaataccag
     1921 aaattccggt tccggggagg tgtaaagggt gagtagcgga aagacagaaa agatatatgg
     1981 catcaacggc ccggtcattt acctgaaagg gaagacggat tttaagatgt cggaaatggt
     2041 ccatgtggga gaggaaaagc tggttggcga agttatttcc ctggataagg accggacgac
     2101 ggtacaggtg tatgaggaga cttccggact gcatccggga gaacttgtgg aaggtacggg
     2161 agcggcagtg tcggttacgc tggcacccgg tatcctgaat aatatctttg acggtattga
     2221 gagaccgctg gagcggatcg ctgacaaggg cggggcattt atcacccgtg gtgtcagtgt
     2281 ggatgcactg gaccgccaga aactctggga gacgcatatc acagtggcgg aaggcgatat
     2341 ggtacagggt ggaacgatca ttgcggaagt gccggagacg cgggctatcg tgcacaaatg
     2401 tatggttcct ccgggagagg aaggaactgt tatatctgtg gttccggatg gtgaatatac
     2461 gattgatgag acgctggtta cgattgaact gttcaatgga gaaaaacgag aactttcgat
     2521 gacacagcac tggccgatcc gtgtaccgag accggtgagc cggagattcc cggcaagtgt
     2581 gccgctggta acaggacagc gaattctgga tacgatgttc ccgattgcaa aaggtggaac
     2641 ggctgcgatc ccgggaggat tcggaacggg taagacgatg acccagcatc agatcgcaaa
     2701 atggtcggat gcggatatta ttatatatat tggatgtggc gagcgtggaa atgagatgac
     2761 gcaggttctg gaagagtttt cagaactggt c
//
LOCUS       DBMDQJ010000308         2791 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12878_length_2791_cov_1.286617, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000308 DBMDQJ010000000
VERSION     DBMDQJ010000308.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12878_length_2791_cov_1.286617"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="ACZO9R_07065"
     CDS             <1..301
                     /locus_tag="ACZO9R_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912860.1"
                     /translation="LVAMNTFAFGIRCAGPFAAYGLGLAVPKATKNSGMISIFTGTIA
                     FVVWQLLSNGGTLWFMMPVVFGSLVSVITFYVVNLIEWKRGVPAAPSAYTVEEAK"
     gene            540..758
                     /locus_tag="ACZO9R_07070"
     CDS             540..758
                     /locus_tag="ACZO9R_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0912861.1"
                     /translation="MILRNRLKERRAALNVNQQEMGRLCGVSRQTISLIERGDYSPSV
                     TLALTIAKVCGVTVEEVFYLQEETENEK"
     gene            748..1551
                     /locus_tag="ACZO9R_07075"
     CDS             748..1551
                     /locus_tag="ACZO9R_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HIR0912862.1"
                     /translation="MKNDEIKNANRKALPKFLLVAVVCAIIGGVIGYYSGHSAAKGGL
                     DQLVGMMKEAGAFFGTHIAPWLMLALAVIVPVVCIPIYRRAKKLVAAWDGEDEDISDT
                     VDRKLSAVIWTTSVALIVSYFLIAASYSGGFATFDSKNSTIIFFIGVVAFFGIMAEAT
                     IIQQKCVDTAKQTNPEKKASVYDMRFQKKWLEDCDEAEKLMIGKCAFKAYSATNAVCT
                     ILAIVLAVCALVFDIGFLPSLTVCLVWIVNLSVYCKEAMRYSKAGCKIS"
     gene            1577..1747
                     /locus_tag="ACZO9R_07080"
     CDS             1577..1747
                     /locus_tag="ACZO9R_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912863.1"
                     /translation="MKLSSTGCFILAVTWLLVSLLWFFWVKNTTIGIVWLIIGAGELV
                     LAFIMRHKETCK"
     gene            1843..2103
                     /locus_tag="ACZO9R_07085"
     CDS             1843..2103
                     /locus_tag="ACZO9R_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912864.1"
                     /translation="MNQKKQNKENSEMKELAKRFIGEECIIYTITSNDGSVQGVIKEI
                     DDGGMVIEKKTGELEIVNLDFVTRIREYPRKKNGRKKGVVLD"
     gene            complement(2155..>2791)
                     /locus_tag="ACZO9R_07090"
     CDS             complement(2155..>2791)
                     /locus_tag="ACZO9R_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912865.1"
                     /translation="RNEYQGLYTSQDPAFIDRVLALHTAAVEEKPRQEQRKTDYQRGT
                     DQTQGFYISYRLTDGRLLQRYYSVVYNEADALASGSLISQFSSVYNSPDCVRIRTGFD
                     TPRTEANVLSCYVYSNASDADLELTGAHAWELYAACLADINAGRLGAEDVSGVGTKDD
                     ADYTPLYLMFIVTTNTPGETVNLYVDSIPLGADETVRVLEQAGAEIYWKTS"
BASE COUNT          607 a          728 c          805 g          651 t
ORIGIN      
        1 cctcgtcgcc atgaacacct tcgcattcgg catccgctgc gcaggcccgt tcgcggccta
       61 cggcctcggc cttgccgttc cgaaggccac gaagaactcc ggcatgatct ccatcttcac
      121 cggcaccatc gccttcgtcg tctggcagct cctgtccaac ggcggtacgc tgtggttcat
      181 gatgccggtc gtcttcggca gccttgtctc cgtcatcacg ttctacgttg tgaacctgat
      241 cgagtggaag cgcggcgtcc ccgcagctcc gtctgcctac accgtcgagg aagccaagta
      301 agactccccc gccccgcctg agggcctgaa cacaagcaag accccggatg atgcgctgca
      361 tcatccgggg gttgtttttc tctgatcgtg ttcctggcgc aaacagacag gcatggcgac
      421 tttatccgta acttttccat gcgttctatt gacaggaagg aattcctgtg gtatcctgtg
      481 ttatgacaat gatagttgtc atttagacaa gaaagattgt caggcaaggg aggtgctgta
      541 tgatactgcg aaatcgttta aaggagcgca gagctgcact aaatgtgaat cagcaggaaa
      601 tgggacgcct atgcggcgta tcgcggcaga cgatcagcct gatcgagcgg ggcgattatt
      661 cgccgtctgt cacccttgct ctgacgatcg cgaaggtctg cggcgttacg gtagaagaag
      721 tcttctattt acaggaggaa actgaaaatg aaaaatgacg aaatcaaaaa cgccaatcgc
      781 aaggctctcc caaagttctt gcttgttgca gtcgtatgtg cgatcatcgg cggcgtgatc
      841 ggttattatt ccggtcacag tgcagcaaaa ggcgggttgg atcaactggt tggcatgatg
      901 aaagaagcgg gagcgttctt cggaacgcac atcgctccgt ggctgatgct ggcgctggca
      961 gtcatcgtgc cggtagtctg catcccgatt tatcggcgtg caaagaaact ggttgctgcg
     1021 tgggatggcg aggatgagga catctcggat acggttgaca gaaagctgtc tgctgtgatt
     1081 tggactacaa gcgtagccct tatcgtctcc tattttctta ttgccgcatc ctattccggt
     1141 ggctttgcaa catttgacag taagaacagc acaatcatct tttttatcgg cgttgttgct
     1201 tttttcggca taatggctga ggcgacgatt attcagcaaa agtgcgtgga caccgccaag
     1261 cagaccaatc ccgaaaagaa agcgtccgtc tacgatatgc gatttcaaaa gaagtggctg
     1321 gaggactgcg acgaggccga gaaactgatg atcggcaagt gcgcgttcaa agcctactcc
     1381 gcaacgaatg cggtctgcac gattttagcg atcgtgcttg cggtctgtgc gcttgtgttc
     1441 gatattggat tcctgccctc gctcacggtc tgcctcgtct ggatcgtcaa tttgtcggtg
     1501 tactgcaagg aggccatgcg ctattccaag gccggatgca aaatctcgta atttttttca
     1561 aaggggggaa cttgtcatga agctcagcag taccggttgt tttattttgg cggtcacctg
     1621 gcttttggtg tcgctgcttt ggttcttttg ggtgaaaaat accacgatcg gcattgtgtg
     1681 gctgatcatc ggagccggtg aattggtcct tgcttttatc atgcgccata aggaaacatg
     1741 caaatgaacg gcagtactta cctcattttg cttgccgtgt tcctttttgt gatcatacct
     1801 tggatcacaa gaagaaggaa actggcggca gtcagacaca ttttgaatca aaaaaagcag
     1861 aacaaggaga acagtgaaat gaaagaactg gcaaaacggt ttatcggcga agagtgcatc
     1921 atttatacga tcacttcaaa tgacggcagc gtacagggag tgataaaaga aattgatgac
     1981 ggcggtatgg tcatagagaa gaagacgggc gaactggaga tcgtcaatct ggattttgtc
     2041 acccggatcc gggagtatcc gagaaagaaa aacggcagga aaaagggcgt cgtactcgac
     2101 tgacaaaccg cccgcgggcc aaagggcccg cgggcggagt ttttgttccg ttcgtcagct
     2161 cgtcttccag tagatctccg cgcccgcctg ctccaggacg cggacggtct cgtccgcgcc
     2221 cagcgggatg gaatcgacgt agaggttcac cgtctcgccc ggggtgttgg tcgtgacgat
     2281 gaacatcagg tagagcggcg tgtaatccgc gtcgtccttc gtcccgacgc cgctgacgtc
     2341 ctccgcgccg agccggcccg cattgatatc cgccagacag gccgcgtaga gctcccacgc
     2401 atgcgcgcct gtcagctcca gatccgcgtc cgacgcgttg gaatacacat agcacgagag
     2461 cacgttcgcc tccgtgcgcg gcgtatcgaa gcccgtgcgg atgcgcacgc agtcggggct
     2521 gttgtagacg gaggagaact gtgaaatgag gctgccggac gccagcgcgt ccgcctcgtt
     2581 gtagaccacg ctgtagtagc gctgcaggag ccgcccgtcc gtcagccggt agctgatgta
     2641 gaagccctgc gtctggtcgg tcccgcgctg gtagtccgtc ttccgctgct cctgccgcgg
     2701 cttttcctcc acggcggcgg tgtgcagggc caggacgcgg tcaatgaacg cgggatcctg
     2761 cgaggtgtag aggccctgat actcgtttct c
//
LOCUS       DBMDQJ010000309         2790 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12883_length_2790_cov_1.180736, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000309 DBMDQJ010000000
VERSION     DBMDQJ010000309.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12883_length_2790_cov_1.180736"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            1..738
                     /locus_tag="ACZO9R_07095"
                     /pseudo
     CDS             1..738
                     /locus_tag="ACZO9R_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823548.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            1191..1298
                     /locus_tag="ACZO9R_07100"
     CDS             1191..1298
                     /locus_tag="ACZO9R_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014950.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0912866.1"
                     /translation="MLTLPKNNGSRQKIAEELEISPTTLWTKMKKGIAE"
     gene            1342..2469
                     /locus_tag="ACZO9R_07105"
     CDS             1342..2469
                     /locus_tag="ACZO9R_07105"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase family protein"
                     /protein_id="HIR0912867.1"
                     /translation="MDVMVLIDSFKGSMTSMEAGNAARDGVLRVYPHANVTVRPLADG
                     GEGTTDALIEGLGGRKVELSVTGPMGSRVKAYYGILADEKTAVMEMAQAAGITLVEEA
                     QRNPGKATTYGVGEMIRDAVSRGCRKFIIGIGGSATNDGGTGMLRALGVKFLTKDGEE
                     AGEGADALGKIHFVSLEHLMPELKECDFQIACDVTNPLCGEKGATCIYGKQKGIREEE
                     MPRIDADMKHYAAITAERIGVDYAAREGAGAAGGMGYAFISYLGARLVPGIELILDVI
                     HFEEEAKKAQIVLTGEGRLDLQTAMGKAPVGVARAAKKYGCKVIAFAGSVTKEAAACN
                     DNGIDAFFPIVRGACTLEEAMQREKAMENLTDSVEQVFRLL"
     gene            complement(2601..>2790)
                     /locus_tag="ACZO9R_07110"
     CDS             complement(2601..>2790)
                     /locus_tag="ACZO9R_07110"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0912868.1"
                     /translation="SVNAYIRRKKIEEAKLLLSGTRMSIQDISDELSFGSRSFFFSSF
                     QKETGMSPTAYRGEHCKG"
BASE COUNT          841 a          512 c          796 g          641 t
ORIGIN      
        1 ttaggttatt ccaagtatga ctatcgaaac aaagaaacag ataacagtcg gaatgggcat
       61 tctagcaaaa ctatgcacac aagctatgga gatatggatg tagctattac aagagatcgt
      121 aacggtgagt atgagcctca gctaatcaaa aaatatcaga atactgtgac tcaggacatg
      181 gaagaaaaaa tcctttccat gtatgcaaag ggaatgacta ccggagatat cgaatctcac
      241 atgcgggaac tgtatgatat tgatatttct gacagtacca tcagccggat tacggataaa
      301 atccttccaa ttgtaaaagg atggcaggaa cgtccgttgg aagaagtgta cgctgtcgta
      361 tttatggatg ctatccacta tcatgtccga agtgaaggac gaattgtaaa acgtgcagtc
      421 tacattgctc ttggcattga tatgaacgga aaaaaggacg ttcttggtat gtatgttgga
      481 gaaaacgaaa gcgccaagtt ctggctctcc atcatgaatg gactaaagaa ccgcggagtt
      541 gaggatatcc tgattgcatg tgtggatgga ctaaatggat ttccacaagc aattgaagct
      601 gtttatccaa aaacagaaat ccagcagtgt atcattcatc agatccgtaa ttcaacgaag
      661 tttgtttcct acaaagatat caaaaagctg atggctgatc tgaagcttgt atatgcggct
      721 ccaacggaag aaacggcatt aataccacca atgcgacctt cgtattaaag ggtatggatg
      781 aaagaagcat accggatgcg ggagacagat atatgaatct tcataataag gcagtgtaaa
      841 taaagtagag taaaacaaca aggaggagct tttaaatgag cgataatcac gacttgttga
      901 atgataatga tatgtgttct ttttcgggtt acgaggtaga cgatgtgtgt tccaacaacg
      961 atatcgacgc atgtggagac ggtatgtttg ttggagtttt tacaaggtgt aaattggaac
     1021 agcgaacatg ataaaaaaat agaaaagtag atataaaggt aacgtgtata gaaaatggtg
     1081 tgacacaagt gcgactggtg gagaaaatcc tattatgagt atgttgagag tgtataagat
     1141 agtttcacgg ttgaaacaga cgtgattatg gatatgtcag tgaagttaat atgctgacac
     1201 ttccaaaaaa taacggaagc aggcagaaga ttgcggaaga actggagatc agccctacaa
     1261 cactgtggac aaaaatgaaa aaaggcattg cagagtgagc attgcaaagt gtctccgctg
     1321 catgcctgta aggagaaaaa gatggatgta atggttttga tcgattcgtt taaggggagt
     1381 atgacttcca tggaagcggg gaatgcggcg cgtgacgggg tgttgcgcgt ctatccgcat
     1441 gccaatgtga cggtccgccc cctggcagac ggaggagagg gcacaacaga tgccctgata
     1501 gaggggctgg gcggaaggaa agtggaactg tctgtgacgg gaccgatggg aagccgggta
     1561 aaagcttact acggaatcct tgcggatgaa aagacggcgg ttatggagat ggcgcaggcc
     1621 gcgggtatta cacttgtgga ggaggcgcag agaaatccgg gaaaagccac cacctatgga
     1681 gtgggcgaga tgatacggga tgccgtttcg cgcggatgcc gcaaattcat cattggaatc
     1741 ggcggaagtg caacaaatga cggcggaacc ggaatgctta gagcgctcgg tgtgaagttt
     1801 ctgaccaagg atggagagga agcaggagag ggagcggatg cgcttggtaa aatacatttc
     1861 gtttcactgg agcatctgat gccggaactg aaagaatgtg attttcagat tgcatgtgat
     1921 gtcacgaatc cgctctgcgg agaaaaaggg gcgacctgta tttatggaaa gcagaagggc
     1981 atacgggaag aggaaatgcc gcgtatcgac gcggatatga agcattacgc cgcgatcact
     2041 gccgagcgga tcggcgttga ctatgcagcc cgggagggcg cgggggcagc cggcggcatg
     2101 ggatatgcct ttatcagtta tcttggggcg agactggttc cgggaatcga gctgatatta
     2161 gacgtgatcc attttgagga ggaggcaaag aaagcgcaga ttgtgctcac cggagaggga
     2221 cggctggatc ttcagactgc aatgggaaaa gcgcccgtgg gggttgcgag ggctgccaag
     2281 aagtatggct gtaaagtgat tgcgttcgcc ggttcggtca caaaagaagc ggctgcgtgc
     2341 aatgacaacg gcattgacgc atttttcccg atcgtgcgag gggcgtgcac gttagaggag
     2401 gccatgcagc gggaaaaagc catggagaat ctcaccgatt cagtggaaca ggtatttcgg
     2461 ctgctataat atcacctatt ctacaggatg atttagaaga acgattatct caaaaatctg
     2521 gaatgaaaga gcaggtgata cgggaaatag atctggataa acggttcaaa aagaaatgtt
     2581 ggggcaatca ggtcctcccc tcacccctta cagtgctccc cgcggtatgc cgtcggtgac
     2641 atgccggtct ccttctgaaa gctggagaaa aagaaactgc ggctgccgaa ggacagttca
     2701 tcgctgatgt cctgaatact catgcgggta cccgatagaa gaagctttgc ttcttctatt
     2761 ttttttcggc ggatataagc attcacgctc
//
LOCUS       DBMDQJ010000310         2788 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12896_length_2788_cov_1.650912, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000310 DBMDQJ010000000
VERSION     DBMDQJ010000310.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12896_length_2788_cov_1.650912"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="ACZO9R_07115"
     CDS             complement(<1..384)
                     /locus_tag="ACZO9R_07115"
                     /inference="COORDINATES: protein motif:HMM:NF014326.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HIR0912869.1"
                     /translation="MDEIDETERQREAAMEIIAKAGAARSCAFAAIHRAKAFDFGGAA
                     EQLDEAERYATEAHKVHTELLVREAGGQQTDGGLLVAHAQDHFMMATLAQELAEEIVE
                     AALRRSGEWAGLDAAETGNAPDGAYR"
     gene            complement(413..2569)
                     /locus_tag="ACZO9R_07120"
     CDS             complement(413..2569)
                     /locus_tag="ACZO9R_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012385.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HIR0912870.1"
                     /translation="MKGNREQIYEFVKLHSSVNANHGISTNEIAEALGMQRTNVSTAL
                     GELLREGRVKKSSSRPVLYSLTEAFADEEGAFRELVGYDGSLKYAVQLARAALLYPHR
                     GLNVLLIGREGSGRKYMAEVMHKAAVQQGLVAEQAPFVMCDCMDFGADDAATIAELFG
                     DGVQGGMLAEAATGILFLHKVQFLSVNVRRKLLRCFVEAEDARELPMIFLSCDDKALD
                     VISLLEDHVLAEIRLPSLTERPLPERRKLLEHFLVAEACRTKRTITLESEVLTCLMLF
                     PCEKEILTLKTQIRKACMNAYLRDYMDHGGELHLYISDFDPSVRKGFLNYRADKEAVD
                     ALVKPQMRYFYQEGGVTVLAPEMPPAEETQVSEPQIRTDEPSPVLLFVFYGQGVARAL
                     SDTIEEQLHRGNVYAVDIPYEQKDEQTYELLRQKLLAIDRGAGVLAVYDQKVLQQMLY
                     TISCETGTQLRAVALPVTRFGMEWSKGAAIADGVDRVYRMAAESLGALWHSPKKVIVT
                     LCTTSEGVALQMKQYLIQHKACTDAEVIALSLSDRNLLREKLLELMQGAVILCIIGTY
                     DPDILAIPFLSAADVLAVPVERLPELLRTQRMEKAEVDFDEMFRCLGDHLEYVDIGRL
                     KEALMELAEQIEGKYPMSLDTKTGLVMHIACAVNRIAGRGVSIENPHREEIVQKYHGV
                     YRDLRKYVAPVERKFHMIFSDDEMANIISILMQIQE"
BASE COUNT          549 a          898 c          619 g          722 t
ORIGIN      
        1 tcgatacgcg ccgtccggcg cgtttcctgt ttccgccgcg tccagtccgg cccattcgcc
       61 ggagcgccgc agcgcggcct ccacgatctc ctccgcaagc tcctgcgcca gcgtcgccat
      121 cataaaatgg tcctgcgcgt gcgccaccag caatcctccg tctgtctgct gtccgcccgc
      181 ctctctcacc agaagctccg tgtgcacctt atgtgcctcc gtggcatagc gttccgcctc
      241 atccaactgc tccgcggctc cgccaaaatc aaatgccttt gctctgtgaa tcgctgcaaa
      301 cgcgcagctt ctcgccgcac ctgccttcgc aatgatctcc atcgccgcct ctctttgccg
      361 ttctgtctca tcgatttcat ccattctgcc cacctccgtg accgcctctg tcttattcct
      421 gaatctgcat caatatggaa atgatgttcg ccatctcgtc atcggaaaaa atcatgtgga
      481 atttccgctc gaccggcgct acatattttc gcagatcccg gtataccccg tgatacttct
      541 gcacaatttc ctcgcggtgc ggattctcta tggaaacgcc ccttcccgca atccggttga
      601 ctgcgcaggc aatgtgcatc acaagccctg tcttggtatc taacgacatc gggtattttc
      661 cctcaatctg ctccgccagt tccatgagcg cctcctttaa gcggccgatg tccacatatt
      721 ccagatgatc gcccagacag cggaacattt catcaaaatc aacctccgcc ttttccatgc
      781 gctgtgtgcg caaaagctcc ggcagacgct ccaccggcac cgccagcaca tccgccgctg
      841 acagaaacgg aatcgccaga atgtccggat catacgttcc gataatacac aggatcactg
      901 ctccctgcat aagttccagc agtttttccc gcagcaggtt ccggtccgat aaggataagg
      961 caatcacctc cgcgtccgtg caggctttat gctggatgag atactgcttc atctgcagcg
     1021 caacaccctc acttgtggtg cagagcgtaa cgattacttt ttttggggaa tgccacagcg
     1081 ccccgagcga ctctgccgcc atccggtaaa cccggtccac cccatcggcg attgccgccc
     1141 ccttcgacca ctccattccg aatcttgtga ccggaagcgc taccgcgcgg agctgtgttc
     1201 ctgtctcgca ggaaattgta tacaacatct gctgcaatac cttctggtca tacacggcaa
     1261 ggactcccgc ccctctatcg atcgcgagca gtttttgccg cagcagctcg taggtctgtt
     1321 catccttctg ttcgtaagga atgtcaacgg catacacgtt tccgcggtga agctgctcct
     1381 cgatggtatc tgagagtgcc cgcgcgactc cctgtccgta aaagacaaat aaaagtaccg
     1441 gcgacggttc gtccgtccgg atctgtggct cgctgacctg cgtctcctcg gcaggcggca
     1501 tttccggtgc aagtaccgtc acgccccctt cctgatagaa atagcgcatc tgcggtttta
     1561 ccagcgcatc caccgcctcc ttgtccgcgc ggtagtttaa gaatcccttg cgcacagacg
     1621 gatcaaagtc gctgatgtag aggtgaagtt ctcccccgtg atccatgtag tcccgcagat
     1681 aagcattcat gcaggctttc cggatctgtg tctttaaggt cagtatctcc ttttcacaag
     1741 ggaacagcat caggcaggtg agcacctcgg actccagcgt gatcgtgcgc ttcgttctgc
     1801 atgcctccgc caccagaaaa tgttccagca gcttccgccg ctctggaagc ggacgctccg
     1861 taagggacgg gagccttatc tccgccagca cgtggtcttc cagcagggaa atcacatcca
     1921 gcgccttgtc gtcgcaggag agaaaaatca tcggcagttc ccgcgcatcc tccgcctcca
     1981 caaaacagcg gagcagcttg cgtctgacgt tcaccgacag gaactgtacc ttatgtaaaa
     2041 acaggattcc cgtggctgcc tccgcgagca ttcctccctg cacgccgtct ccgaacagtt
     2101 ctgcgatcgt tgccgcatcg tccgctccga aatccatgca gtcacacatt acaaacggcg
     2161 cctgttccgc aaccagtccc tgctgtacgg cagctttgtg catcacttcc gccatgtatt
     2221 ttctgccgct tccctctctt ccgatcagaa ggacattcag tccgcggtgc ggatacaaaa
     2281 gtgccgccct ggcaagctga acggcgtatt ttaaactgcc gtcatatccc accagttccc
     2341 ggaacgctcc ctcctcatcc gcaaacgctt ccgtcagcga atataagacc ggccgactgc
     2401 tgctcttttt taccctgcct tcccggagga gttcgcccag tgcagtgctg acgttggtgc
     2461 gctgcattcc gagtgcctct gcgatctcat tagtagagat cccgtgattg gcattcacgg
     2521 agctgtggag ttttacaaat tcgtatatct gctctctgtt tcccttcata tggttcctca
     2581 taagaaatcg ctgatctctt cgatacgctt actatatgtt ttttctttct gactgtcaat
     2641 ttcacatagt gtataaattt tgtatccttc atctatactc attttcatac aaaatgcttt
     2701 ctttttcaac tctctttttg tctgtttttt ccaataatcc gcttcttttc ttcctgtcgc
     2761 ttctttctcc gcttatttag tgtatatg
//
LOCUS       DBMDQJ010000311         2787 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12903_length_2787_cov_1.797468, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000311 DBMDQJ010000000
VERSION     DBMDQJ010000311.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12903_length_2787_cov_1.797468"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="ACZO9R_07125"
     CDS             <1..796
                     /locus_tag="ACZO9R_07125"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HIR0912871.1"
                     /translation="KSVLDNVCFPMQIAKKKKDEAKKRALELLETVGLADKADAYPAQ
                     LSGGQKQRVAIARALATDPKILLCDEATSALDPQTTQSILALLKEINRKFGITIVIIT
                     HQMSVIREICSHVAIVEKGSIVEVGLVEEIFNHPKSRVARSLILSDRAADAASYSGAE
                     GDTADGAELTGWSGESRQRDRIGDDKKLRIVFSENSAFEPVIANMVLKFGTPVNILRA
                     DTKNVGGVAKGEMILGLPSDAGLQEQMEAYLVEKGLEIEEVTGDVE"
     gene            786..1445
                     /locus_tag="ACZO9R_07130"
     CDS             786..1445
                     /locus_tag="ACZO9R_07130"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HIR0912872.1"
                     /translation="MWNSQVIIMLLEGIRDTLYMTLASTLFGYIIGLPMGIVLTVTDK
                     DGIRPNAAVYKVLDVISNLLRSIPFIILLIVLIPFTRFVVGRSYGSTATIVPLTIAAA
                     PYIARMVESSLKEVDAGVIEAARSMGASDFQIVTKVMLVEARTSLIVGATISLGTILG
                     YSAMAGTVGGGGLGDIAIRYGYTRWQTDIMVVTVVLLVILFQIFQTIGMKIANRLDRR
                     K"
     gene            1493..2413
                     /locus_tag="ACZO9R_07135"
     CDS             1493..2413
                     /locus_tag="ACZO9R_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR0912873.1"
                     /translation="MKKKVLSTILAGVLALSAFAGCGSSTEESTDTSAATKEETPVAA
                     TEEAVSAENTESTDTAAEGGTIKVAASATPHAEILEEAKKILADEGWDLEIKIFDDYV
                     MPNEVVESGEFDANYFQHIPYLESFNEEKGTHLVNAGGIHYEPFGIYPGTKSDLSELA
                     DGDTIAVPNDTTNEARALLLLQDNGIITLKEGAGLEATVNDIAENPHNVKIEELAAEQ
                     VARVAPEVAFVVLNGNYALQAGFSVAKDALAYEASDSEAAKTYVNIIAVKEGHENDPG
                     IQALVKVLKSDEIKQYINDTYDGAVIPFED"
     gene            complement(2473..2607)
                     /locus_tag="ACZO9R_07140"
     CDS             complement(2473..2607)
                     /locus_tag="ACZO9R_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912874.1"
                     /translation="MECSKLGKTRIIEDASCGMVRNTGFCGQNLPPHGYLELLSIYCS
                     "
BASE COUNT          748 a          618 c          795 g          626 t
ORIGIN      
        1 aaagagtgtg ctggacaatg tatgtttccc gatgcagatt gcgaagaaaa agaaggatga
       61 ggcgaagaaa agagcgcttg agctgctcga gacggtcgga cttgccgaca aggcggacgc
      121 ttatccggca cagctttccg gcggacagaa gcagagagtg gcgatcgcca gagctcttgc
      181 gacggatccg aagattctgc tctgcgacga ggcaaccagt gccttagatc cgcagacaac
      241 gcagtccatt ctggcactct taaaggagat caaccggaaa ttcggcatca cgattgtgat
      301 tatcacgcat cagatgtccg tgatccggga aatctgcagc catgtggcga ttgtggaaaa
      361 aggaagcatc gtggaagtcg gactggtcga ggagattttc aaccatccga aatcccgtgt
      421 ggcgagaagc ctgatcttga gcgaccgtgc tgcggatgcc gcgtcatatt ccggggcgga
      481 aggtgatact gcggatggag cggagctgac cggatggagc ggtgagagca gacagcggga
      541 ccgcatcggc gacgataaga agcttcgtat tgtattctca gagaattccg catttgagcc
      601 cgtcattgcc aatatggtgt taaagttcgg tacgccggtg aacatcctcc gtgcagatac
      661 caagaatgtc ggcggagtgg caaagggtga gatgattctg ggacttccgt cagacgccgg
      721 gctgcaggag cagatggagg cttatctggt ggaaaaggga cttgagattg aggaggtgac
      781 cggagatgtg gaatagtcag gtaatcatca tgcttttgga gggaatccgg gacaccctgt
      841 atatgacctt agcgtccaca ttgtttggat atatcatcgg acttccgatg ggaattgtgc
      901 tgactgtcac ggacaaggac ggcatccgtc cgaatgcggc agtctacaag gtgctggatg
      961 tgatttccaa cctgctgcgc agtattccgt ttattatttt gctgattgtg ctgattccgt
     1021 tcaccagatt tgtggtggga agaagctacg gatcgacggc gacgatcgtc ccgctgacga
     1081 ttgcggcggc accttacatt gccagaatgg tagaatcctc attaaaagag gtggatgcag
     1141 gcgtcatcga ggcagcgcgc tccatgggag catccgattt tcagattgtg accaaggtca
     1201 tgctggtgga ggcgagaacg tctctgattg tcggagcaac gatttccctt ggcacgattt
     1261 taggatattc cgcgatggca ggaaccgtcg gcggaggcgg actgggagat attgccatcc
     1321 gctatggtta caccagatgg cagaccgata tcatggttgt cacagtggtg cttctggtaa
     1381 ttttattcca gattttccag acgatcggaa tgaagattgc gaatcggctg gatcgcagaa
     1441 agtagcaata acaaatttgc atatattttt aagttttaag gaggacacta ttatgaaaaa
     1501 gaaagtattg tcaacgattc tggcaggcgt gctcgcatta tccgcattcg caggatgcgg
     1561 aagcagcacg gaggagagca cagacaccag tgcagcaaca aaggaagaga cgcccgtagc
     1621 tgcaacggag gaggctgtga gtgctgagaa cacagagagc accgataccg cagcagaagg
     1681 cggcaccatc aaagtggctg catccgcaac accgcacgca gagattcttg aggaagcaaa
     1741 gaagattctt gccgatgagg gatgggatct ggagatcaag attttcgacg actatgtaat
     1801 gccgaacgaa gtggtagaaa gcggcgagtt tgacgcaaac tacttccagc acattccgta
     1861 ccttgagagc tttaacgaag agaagggaac ccatctggtc aatgcaggcg gcatccacta
     1921 tgagccgttc ggcatttatc cgggaacaaa gtccgatctc tccgagctgg ctgacggcga
     1981 caccatcgca gtgccgaacg ataccacaaa cgaggcgaga gcacttcttc tgttacagga
     2041 taacggcatc attaccttaa aggaaggcgc cggcttagag gcaactgtta atgatatcgc
     2101 agagaacccg cacaatgtca agatcgagga gctggcagca gagcaggttg caagagtggc
     2161 tccggaggtt gcattcgttg tattaaacgg taactatgca ttacaggcag gcttctccgt
     2221 tgcaaaagat gcactggctt acgaggcatc cgattccgag gcagcgaaga cctacgtgaa
     2281 catcattgca gtaaaagagg gacatgagaa tgaccctggc atccaggcac ttgtaaaagt
     2341 attaaagtct gatgagatca agcagtacat caacgacacc tatgacggag ccgttatccc
     2401 gtttgaggac taagcaaata gaggaaagaa ttgtcgaaat cagcatattg tgttttgtgt
     2461 cgataagtaa tattacgaac aatagatact taaaagttcc agataaccat gcggcggcaa
     2521 attctgaccg caaaatccgg tatttctaac catgccgcag ctggcatcct cgatgatccg
     2581 tgtctttcca agtttggagc actccacgga ctgtgtccgc aagccagcag acaattttag
     2641 gacttttgtg tgtgtccctt attggcacct ctgaacaaaa cataaccgcg cgaagccgat
     2701 gtttgaattg ccatctgcca tatttttaat agcagatggc aacgagtttc ggttcgcgcg
     2761 gttttatatt gtctgtgaag aggtgcc
//
LOCUS       DBMDQJ010000312         2779 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12949_length_2779_cov_1.467513, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000312 DBMDQJ010000000
VERSION     DBMDQJ010000312.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2779)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2779)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2779
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12949_length_2779_cov_1.467513"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1374)
                     /locus_tag="ACZO9R_07145"
     CDS             complement(<1..1374)
                     /locus_tag="ACZO9R_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR0912875.1"
                     /translation="MNHFIYRKKRKILLAAISMGVLAASISAFHGPEAVYAYTATAGT
                     INDGPVKVRNAPVTGDKLTLLYPGNAVTVTDETAGSDGYTWCQIQMSDGVTGYIRSDF
                     VTLQGTDSSAGQTNADSSAGGTNGVVQNTEKGVYVRSGAGTSYSVVTKVYKGQAVNVG
                     ELVSAGGMNWYSVTLVVGGASYSGWICADYVAVNGQTSDDQSQGETTGATDSEYEAAL
                     AAAGFPASYCSALASLHQKYPNWQFVPVQTGLDWNTVVSNESLVGRNLIQNSVNDARK
                     STDSQAYNWETNKWYGFDGASWVSASPEYIAYCIDPRNFLNENQIFQFETLEYAGYQN
                     AAGVQSVLSNTFMAGNYTDTDGAVRSYADTFVEIGSNVGVSPYHLASRCKQEQGVRGT
                     SDLISGRYSNYAGYYNYFNVRAFTTGSASAIVNGLEYAKLQGWNANSTTAVAAIADNI
                     IVKMQEKC"
     gene            complement(1501..1713)
                     /locus_tag="ACZO9R_07150"
     CDS             complement(1501..1713)
                     /locus_tag="ACZO9R_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912876.1"
                     /translation="MKTVDMELILLCALMAAIGAFGLVTGVVLLFGIQFQECNAALLT
                     GIVCGLWGILWGSLLIQNRNLVSKNV"
     gene            complement(1731..2084)
                     /locus_tag="ACZO9R_07155"
     CDS             complement(1731..2084)
                     /locus_tag="ACZO9R_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912877.1"
                     /translation="MEKKIEIIAVDFDGTLCSECYPAIGMPNLPLFALLKGARRQGKK
                     VILWTCRCGDLLEEAVAWCRMFGLEFDAVNENVAQTLEQYGTESRKISADVYIDDKAC
                     WPWMTKQEWEQYLAG"
BASE COUNT          637 a          836 c          579 g          727 t
ORIGIN      
        1 gcatttttcc tgcattttca caatgatatt gtcagcaata gccgcaactg ctgtggtaga
       61 attggcgttc catccctgca acttcgcata ttccagtccg ttcacaatcg ccgacgcgct
      121 gcccgtcgta aacgcacgga cgttaaagta gttataatag cctgcatagt tggaatatcg
      181 tcccgaaatc agatcgctcg tcccccgcac gccctgctcc tgcttacagc gggatgccag
      241 gtggtaaggg ctcacgccca cattggagcc gatctccaca aacgtatccg catagctgcg
      301 aaccgctccg tccgtatcgg tataattgcc tgccataaat gtattggaaa gaacgctctg
      361 cacacccgct gcgttctgat agcctgcata ttccaacgtt tcaaactgaa agatctgatt
      421 ctcgttaaga aaatttctgg gatcgatgca gtatgcgata tattccggag acgccgacac
      481 ccagcttgcc ccgtcaaacc cgtaccactt gttggtctcc cagttatacg cctgactgtc
      541 cgtcgatttt cttgcgtcat tcacggaatt ctggatcaag tttcttccga ccagactctc
      601 attcgatacc accgtattcc agtcaagtcc cgtctgcacc ggcacaaact gccagttcgg
      661 atacttctga tgcagggatg ccagtgcgct gcaatagctt gccggaaaac ccgctgccgc
      721 aagcgccgcc tcatactcgc tgtccgtggc accggtcgtc tctccctgcg actggtcgtc
      781 tgacgtctga ccgttcacgg ctacatagtc cgcacaaatc cacccggaat aggaggcgcc
      841 tccgaccacg agcgtcacac tgtaccagtt cattccgccc gccgagacca gctctcccac
      901 attcacagcc tgtcctttgt atacctttgt caccacagag taggacgttc ccgctcccga
      961 gcgcacataa acaccttttt ccgtgttctg caccaccccg tttgtcccac ctgcactgct
     1021 gtccgcattc gtctgtcccg cagaagaatc cgtgccctga agcgtcacaa aatccgagcg
     1081 tatataaccg gttacaccat ccgacatctg gatctggcac catgtatacc cgtctgatcc
     1141 tgccgtctca tcggttactg tcaccgcatt tcccggatag agcagtgtca gcttatcccc
     1201 ggtcaccggt gcgttgcgca ccttcaccgg tccgtcgttg atcgttcccg ccgtcgctgt
     1261 gtacgcgtat acggcttcgg gtccgtgaaa agcagatatg ctggcagcaa gcactcccat
     1321 gctgattgcc gccagcaata tctttctctt ctttctatat ataaagtggt tcatcgaatt
     1381 cctccctgag taccgcataa atccataaaa tcatgcgccc aataattcag tctcattata
     1441 ggagattcta tcacacgcgt caacctaaat atagccattt tcctgcacgc tgtactatat
     1501 ttagacgttt ttcgacacca ggttccggtt ctgtatcagc aggcttcccc ataatatgcc
     1561 ccagagcccg cacacaattc cggtgagcag tgcagcattg cactcctgaa actggattcc
     1621 gaacagcagc accacacccg tcaccagtcc gaacgctcca atcgccgcca taagcgcaca
     1681 caaaagtatc aattccatat ccactgtttt catcttctta tcctcctttt ctaacctgcc
     1741 agatactgtt cccactcctg cttcgtcatc cacggccagc acgccttgtc atcgatatag
     1801 acgtccgcgg agatttttct ggattccgtt ccatactgtt ccaacgtctg cgccacattt
     1861 tcatttaccg catcaaattc cagtccgaac attctgcacc aggccaccgc ctcctccagc
     1921 agatccccgc agcggcatgt ccacaaaatt acttttttgc cctgccttct cgcacccttg
     1981 agcaatgcaa agagcggcag attcggcatc ccgatggcag gataacattc actgcacaat
     2041 gtcccgtcaa aatcgacggc aatgatctcg atctttttct ccatgttctt ccctccccat
     2101 ccgctttgat ggtgtcagta tcgcacatga tgtgtccaat tctttggaca cttgttatgt
     2161 aaccccaaat ttataggata aaaattgtcg cacgaagcac agacctcttt ggggtgataa
     2221 gtaatattat gagaagaccg caccgtggga tttcagatgg aaatgcaggt ggaaattcgg
     2281 ttcaaaaaat gtggcatttc taaccagccg gatgtttgca tctgcgatga tccgtgtctt
     2341 tccaagtttg gagcactccg cgggtcatgt ccgcatcccg gagacaattt gtaggacttt
     2401 cgtgtgtgtc ccttattggc acctctgaac aaaacataac cgcgcgaagc cgatgtctga
     2461 gtcggcatct gccttatttt caatagcaga tgccggcgag tttcggttcg cgcggtttta
     2521 tattgtctgt gaagaggtgc cattagggac actagaaagt ccgcccattg tctccggtgt
     2581 gccggacatg cacccgtcgg attgtccaaa cgtggaagag acacggaatc cgatgcaaac
     2641 attcagccgg aagaaatgct cacatttttt gaaccgaatt tccatctgca tttccacatg
     2701 aaatccccaa gtgtgtttcc tgtaatatta cttatcaaac caaagcacgg tatgcttcgt
     2761 gcgacacctc ctctatttt
//
LOCUS       DBMDQJ010000313         2774 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12976_length_2774_cov_1.558174, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000313 DBMDQJ010000000
VERSION     DBMDQJ010000313.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12976_length_2774_cov_1.558174"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            6..1229
                     /locus_tag="ACZO9R_07160"
     CDS             6..1229
                     /locus_tag="ACZO9R_07160"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR0912878.1"
                     /translation="MENAAVANVLRGEGYRYCHTNLAEAGIYYKYYQEGFHIVMAVDL
                     SGGNRFSVQQYRSMEEHAMDLFYHPQGRLGDFPDGFPVYHVEMLTLLIGGDSEVERKL
                     CAECRNVWVYQPQSGRLLIYENQPGDFFDLRRALEQGVGGGGTGETSWHGTGTGGAGE
                     TSWHGAGTGGRRQTPLHGAGAGGWGATPRQGTGAAYASAQNTAKNRLGILWKNIRAEY
                     VTIALVVVNVITYLVLEWLGDTTNGFFMAEHGAMYPDFIRINHEWWRIITAGFLHFGA
                     VHLVNNMVILYCMGSRLERVTGHLKYFLIYLVSLIGAGLLSYGMMLRTGDYAVSAGAS
                     GAIFGVIGGFLWIVILHRGRFEQITTRGIMMMIVLTIYYGFSSAGIDNWGHIGGLLAG
                     FSATVILYHRNRQKY"
     gene            1306..2298
                     /locus_tag="ACZO9R_07165"
     CDS             1306..2298
                     /locus_tag="ACZO9R_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HIR0912879.1"
                     /translation="MKVNIYYGGRGLLDDPTLYVLNKMEEVLRELRVMVERINIYEHK
                     NEIATLPQTMKEADGIILATTIEWLGIGGYMQQFLDACWLYGDKEKIQTTYMQPIVMS
                     TTYGEREGEMTLANAWEILGGLPCPGLCGYVEDLVSFEMNKDYTVIIEKKAENLYRTI
                     SQKMKSLPNSNLAVRQSVLRTPQLELTPQESEQLSKYVSDDSYVKKQKEDIQELASMF
                     KDMLGKVSQEDDVAYIDQLRNAFVPQQDFHARYLFMFADKKKPLFIEVKDEDLTVRYG
                     QEENIDIYAKLTPEVMDGVIAGRMTFQRAFMTGEMTAKGNFKILRMLDTVFNFA"
     gene            complement(2285..>2774)
                     /locus_tag="ACZO9R_07170"
     CDS             complement(2285..>2774)
                     /locus_tag="ACZO9R_07170"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912880.1"
                     /translation="MVIQLSSARLFNHLNLMQVNIYSFMHQIDTSIRVLSWKNFVCNF
                     QLEENLPLIELDPQLLKQAVINIIKNAFEAMHESGTTDVHVFYEEPFMSIAITDHGGG
                     LDPALADTIFQPFITSKTGGSGLGLVITRQIIESHHGTLTCDSRPGDGCTFTIALPLT
                     QN"
BASE COUNT          722 a          544 c          834 g          674 t
ORIGIN      
        1 cgcgtatgga aaatgcagca gtcgcaaatg tattaagagg agaaggctac cgctattgtc
       61 acaccaatct ggcggaagcc ggaatttatt ataagtatta tcaggagggg ttccatattg
      121 taatggcggt agatctctcc ggcggaaacc ggttctctgt gcagcagtac cggagcatgg
      181 aagaacacgc catggatctg ttctatcatc cgcaggggag acttggggat ttcccggatg
      241 gatttccggt atatcatgtg gagatgctga cacttctgat cggcggagat tccgaggtgg
      301 aacgaaagtt gtgcgcagag tgcaggaatg tgtgggtata tcagccccaa agcggacggc
      361 tgctgatcta tgagaaccag ccgggagatt ttttcgacct ccggagagca ttagagcaag
      421 gcgtcggggg cggcggcacg ggagagacat catggcatgg caccggaaca ggtggcgcgg
      481 gagagacatc gtggcatgga gccggaacgg ggggccggag acagacgccg ctgcatggag
      541 ccggagccgg tggctgggga gcgacgccgc ggcaaggtac aggtgccgca tatgcgtctg
      601 cacagaatac ggcaaagaac cggttgggca ttttgtggaa gaacatccgg gcggaatatg
      661 tgaccatcgc tctcgttgtt gtcaatgtta taacatatct tgtgcttgaa tggctcggag
      721 atacgacgaa cggattcttt atggcggagc acggcgcgat gtacccggat tttatccgga
      781 tcaaccatga atggtggagg atcataactg ctggcttcct tcatttcggc gcggtacatc
      841 tggtgaataa tatggtgatt ttatactgta tgggatcaag actggagcgt gtcacagggc
      901 atctgaagta ttttctgatt tatctggttt ccctgatcgg ggcgggactg ctctcctacg
      961 ggatgatgct tcgcaccggc gattatgcag tgtcggcagg agcttcgggt gctattttcg
     1021 gagtgattgg cggattcctg tggatcgtga tactgcaccg gggcagattt gagcagatta
     1081 caacccgggg aattatgatg atgattgtac ttaccatcta ttacgggttt tcctcggcgg
     1141 gaattgataa ctggggacac atcggaggtc tgcttgccgg attttccgcg acagtcattt
     1201 tatatcacag aaaccgtcaa aaatattgat tttgtgagca aaaatttata tactgaaagc
     1261 agtatgttcc ataaagcgac agatgggaca aaacggaggc tttgaatgaa agtaaatatt
     1321 tattacggtg gacggggact gttggacgat ccgacacttt atgttttgaa taagatggaa
     1381 gaagtgctgc gcgaactgcg tgtgatggtc gagcggatca atatctatga gcacaagaat
     1441 gaaatagcga ccctgccgca gacgatgaaa gaggcggacg gtattattct tgcgaccacg
     1501 atcgagtggc tcggaatcgg cggctacatg cagcagtttc tggatgcatg ctggctgtac
     1561 ggcgataagg agaagattca gacgacctat atgcagccga tcgtgatgtc tacgacttac
     1621 ggagagcgtg agggtgagat gacgttggca aatgcatggg agattctcgg cgggcttcct
     1681 tgtccgggac tttgcggata tgtggaggat ctggtgtcgt ttgagatgaa caaggactac
     1741 acggtcatta ttgagaaaaa ggcggagaac ctctaccgga cgatttctca gaagatgaaa
     1801 agtctcccga acagtaacct cgcagtcaga cagagtgtgc tgcgcacacc gcagctggaa
     1861 ctcacgccgc aggaaagtga gcagctgtcc aagtatgtat cggatgacag ctatgtgaag
     1921 aagcagaagg aagatattca ggaactggca tccatgttca aggatatgct ggggaaggtc
     1981 agtcaggaag atgatgtggc ctatatcgat cagctgcgca atgcgtttgt tccgcagcag
     2041 gattttcatg cgagatatct gtttatgttt gcagataaga aaaagccgct ttttattgaa
     2101 gtgaaggatg aggatcttac ggtacgatac ggtcaggaag agaatattga tatctatgca
     2161 aaattgacac cggaagtaat ggatggtgtg attgccggga gaatgacatt ccagagagcc
     2221 tttatgacag gcgagatgac tgcgaagggc aattttaaga ttctgcggat gctggatacc
     2281 gtgttcaatt ttgcgtaaga ggaagtgcga tggtgaaggt gcatccgtca cccggtctgg
     2341 aatcgcaggt taatgtaccg tggtgtgatt cgatgatctg acgggtaatg acaagtccca
     2401 gaccgctgcc tcccgttttg gaggtaataa aaggctggaa gatggtatcc gccagagccg
     2461 ggtcgagtcc gccgccgtgg tcagtgatgg caatgctcat aaagggttct tcataaaaga
     2521 cgtggacatc ggtagtgcca ctctcatgca tggcttcaaa tgcatttttg ataatattta
     2581 tgacagcttg ttttaaaagc tgcggatcca gttcgatcag gggaaggttc tcttcaagct
     2641 ggaagttaca gacaaagttc ttccaggaca gtacacggat ggaagtgtcg atctgatgca
     2701 tgaaagaata gatgtttacc tgcatcaggt ttaaatgatt aaaaagacgc gcagacgaca
     2761 gttggatgac cata
//
LOCUS       DBMDQJ010000314         2771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12992_length_2771_cov_1.716105, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000314 DBMDQJ010000000
VERSION     DBMDQJ010000314.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12992_length_2771_cov_1.716105"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /locus_tag="ACZO9R_07175"
     CDS             complement(<1..1418)
                     /locus_tag="ACZO9R_07175"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR0912881.1"
                     /translation="MSYMETYKEWCTNPYFDENTKAELAAIGDDAAEIEDRFYRQLEF
                     GTGGLRGVIGAGTNRMNIYTVRQATQGLANYILSQNGQEKGVAIAHDSRIMSTEFADE
                     AALCLNANGIKAYVFDSLRPTPELSFAVRELGCISGIVITASHNPREYNGYKVYWEDG
                     AQITPPHDKNILAEVAKVTSFDQVKTMKKEDAVAAGLYKVIGKEIDDRYMEELKKQSI
                     HPEVIKEMAKDIKIVYTPLHGTGNLPVRRVLKELGFEQVYVVKEQELPDGNFPTVSYP
                     NPESPKAFELALKLAKEVDADIVLATDPDADRLGVYCKDTKTGGYVTFTGNMSGMLIA
                     EYILREKTAMGTMPENPALVETIVTTDMAKAIAAAYNVKLIEVLTGFKYIGEQIKFFE
                     QQHTYHYVFGLEESYGCLAGTYARDKDACVAVMMLCEVASWCKKHGKTLWDAMLDMYE
                     KYGYYREGLETKTLKGIDGAAQ"
     gene            complement(1520..2746)
                     /locus_tag="ACZO9R_07180"
     CDS             complement(1520..2746)
                     /locus_tag="ACZO9R_07180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR0912882.1"
                     /translation="MNKKCRILLYVLSIVMVAGCITAVARTKGSTPEVKVPGEEPAAV
                     VSGEDTEHAGADSGADRAPAREENETAVETTEAEERTEAAEVAEEAEAGGQAEADGST
                     TILFTGDVLFANAFKAGYDADGIKGVVAPELLEELRAADILMVNNEFPFSDRGTPMAD
                     KQFTFRCSPGYVKALNEMGVDVVSLANNHTLDYGREALSDTFAALDGAGILYGGAGDS
                     AERAKQVQVIEVHGKKYGFIAVSRVVPTADWKVENAVPGLFSCYDTTALVEVIKEARE
                     TCDYVAVYPHWGVEYQAYPEANQTQIAKACIDAGADVVVGSHTHCLQGVSYIDGKPVF
                     YSLGNFIFGQSIDRSAMLEVTIDAAGRASYRFVPVYAAGGVTYPAEGGQAESVLNYLD
                     GISEAAVASDGTVTED"
BASE COUNT          542 a          908 c          600 g          721 t
ORIGIN      
        1 atctgtgccg caccgtcgat tccctttaag gtcttcgtct ccaatccctc tctgtaatag
       61 ccgtacttct catacatatc cagcattgca tcccacaggg tcttgccgtg cttcttgcac
      121 caggaagcca cctcgcaaag catcatgacc gccacgcacg catccttgtc tctcgcgtag
      181 gttcctgcca ggcagccgta gctctcttcc agtccgaata cgtagtgata cgtatgctgc
      241 tgctcgaaga acttgatctg ctctccgata tacttaaatc cggtcagcac ctcgatcagc
      301 ttcacgttgt aggctgccgc gatcgctttt gccatatcgg tcgtcacgat cgtctccacg
      361 agcgccgggt tctccggcat ggttcccatc gctgtcttct ctcttaagat gtactctgcg
      421 atcagcatac cggacatatt tccggtaaat gtcacatacc cgccggtctt tgtgtccttg
      481 cagtacacgc caagacggtc tgcatccggg tctgtcgcga gcacgatgtc cgcatcgact
      541 tcttttgcca gctttaacgc aagttcaaat gccttcggag actccgggtt cgggtaggat
      601 accgtcggga aattaccgtc cggcagctcc tgctctttta ccacgtacac ctgctcgaat
      661 ccaagctcct ttaacacgcg gcgcaccgga aggttgccgg ttccgtggag cggtgtgtat
      721 acaatcttaa tgtccttcgc catctccttg atgacttccg gatggatcga ctgcttcttc
      781 agctcctcca tgtaacgatc gtcgatctct tttccgatga ccttgtaaag tcctgcggca
      841 acggcatctt ctttcttcat cgtcttcacc tggtcgaaag atgtcacctt cgccacttcc
      901 gccaggatat tcttgtcgtg cggcggtgtg atctgtgcgc cgtcctccca gtataccttg
      961 tagccgttgt attctctcgg gttatggctc gccgtgatca caattccgga aatacagccg
     1021 agttcacgca cggcaaagga aagctccggc gtcggacgca ggctgtcgaa cacatacgcc
     1081 ttgatgccgt ttgcgttcag gcagagcgcc gcctcatccg caaattcggt ggacatgatt
     1141 ctggaatcgt gggcgatcgc aacacccttc tcctgtccgt tctgggagag aatgtagttc
     1201 gccagtccct gtgtcgcctg acgcacggta taaatgttca tgcggttcgt tcccgctccg
     1261 atcacgccgc gcagtccgcc ggttccaaac tctaactgac ggtaaaaacg atcctcaatc
     1321 tccgccgcat cgtcccctat tgcagcaagt tctgcctttg tattctcatc aaaatacggg
     1381 tttgtgcacc actctttgta agtctccatg taactcatat ctcttcttct ccttttactg
     1441 atattcttct gccgcgcaga aggaagcttt cttgctttaa attataaact tttttaaact
     1501 gattgtaaac taattttagt taatcttctg tcacggttcc atccgatgcc acagctgctt
     1561 ccgaaatgcc gtccagataa ttcaaaaccg actctgcctg tccgccttcg gcaggatatg
     1621 tcacgcctcc cgccgcatac accggcacga accggtagga tgcccgcccc gcggcgtcga
     1681 tcgtcacctc aagcatcgcc gaccggtcga tactctgccc gaagataaaa ttgccaagac
     1741 tgtagaatac cggcttcccg tcgatatagg agaccccctg cagacagtgg gtgtgcgatc
     1801 ccacaaccac atccgccccg gcgtcgatac acgccttcgc aatctgtgtc tggttcgcct
     1861 ccggatatgc ctggtactcc acgccccagt gcggatagac cgccacatag tcgcaggtct
     1921 ccctcgcttc cttaatgacc tctacaagtg ctgtggtgtc atagcagcta aacagtcccg
     1981 gcactgcatt ttccaccttc cagtccgccg tcgggacaac ccgggatacc gcgataaatc
     2041 cgtatttttt cccgtggact tcgattacct gcacctgctt tgcccgctcc gcggaatcgc
     2101 ccgcgccgcc atatagaatt ccggcaccgt ctaacgctgc aaacgtatcc gagagcgctt
     2161 cccttccata atcaagtgtg tggttgttgg cgagtgatac cacatccacg cccatctcat
     2221 ttaatgcctt tacatagccc ggactgcacc ggaatgtaaa ctgtttgtcc gccatcggcg
     2281 tgccgcgatc cgagaacggg aattcattat tcaccatcag aatatccgct gcccgaagct
     2341 cctccagcag ttccggtgca accacgccct tgattccgtc cgcatcataa cccgccttaa
     2401 acgcatttgc aaacaggaca tccccggtaa aaagaatcgt cgtactgcca tccgcttccg
     2461 cctgaccgcc cgcttccgct tcttcggcca cttccgcggc ttccgttctc tcctccgcct
     2521 ccgtggtctc caccgcggtc tcattctcct ccctggcagg cgcacgatcc gctccgctat
     2581 ccgcccccgc atgctccgtg tcctcacccg ataccacagc tgccggctct tcgcccggca
     2641 cctttacttc cggcgtgctc cctttcgtcc gcgccacggc agtaatacat cctgccacca
     2701 tgacaatcga cagcacatac aataggattc tgcacttttt attcataaaa ttcctccctt
     2761 attcttctct c
//
LOCUS       DBMDQJ010000315         2771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12993_length_2771_cov_1.689139, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000315 DBMDQJ010000000
VERSION     DBMDQJ010000315.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12993_length_2771_cov_1.689139"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /locus_tag="ACZO9R_07185"
     CDS             <1..591
                     /locus_tag="ACZO9R_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HIR0912883.1"
                     /translation="IIFNGNGYDDAWIKEATEKRGLMNYPSTPDCMPHLLDEKNVRML
                     TSHKVFSKAELESRCEIMLENYCKTIVIEANTMVDMVKREIIPAVEAYAMELARTASA
                     KKRVDETLACRYESGLIKKLSTLTDQMAVRTDALETALIELQDVKDIVAQSYQIRDTV
                     LTRMQELRIACDEAETVTAKKYWPFPTYGDLLFSVK"
     gene            620..2332
                     /locus_tag="ACZO9R_07190"
     CDS             620..2332
                     /locus_tag="ACZO9R_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR0912884.1"
                     /translation="MYSSVNTIWVLFGTALVFFMQAGFSLCEAGFTRAKNTGNILMKN
                     LMDFCIGTPAFWLVGFGLMFGKGSGIIGSFDPLIRGEYSQALPSGVPLWAFAIFQTVF
                     CATSATIVSGAMAERTKFSAYCIYSAAISLLIYPVSGHWIWGGGWLSQMGFHDFAGST
                     AVHMVGGICAMIGAKILGPRIGKYDKNGKPQAILGHNLTFAALGVFILWFCWFGFNGA
                     STVGMDSDALIETAGRVFFNTNLAAAVACCAALIFTWIRYKKPDVSMTYNAALAGLVG
                     ITAGCDAVDAVGAAVIGVVCGILIVLAIEFFDKIAKIDDPVGAVSVHCVCGAAGTVLT
                     GLFATGETTEAGLFYGGGAHFLGIQVLGVLAVAAYVAVVITIVFLAIKHTIGLRVKPE
                     EELAGLDVSEHGLFTAYAGFSMLPDTIAEDVDTNTVFAMGDVPEAEAVPVKTVPAFEE
                     GTPKFTKVEIVCKESKLEALKAAMMNLGITGMTVSHVLGCGVQKGKPEYYRGVQIEAN
                     LLPKIQVEIVVSKVPVRSVIETAKKVLYTGHIGDGKIFVYDVENVVKIRTGEEGFDAL
                     QDVE"
     gene            2398..>2771
                     /locus_tag="ACZO9R_07195"
     CDS             2398..>2771
                     /locus_tag="ACZO9R_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004841411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HIR0912885.1"
                     /translation="MCSIMGYCGPVVDLDKFKEGFQRTISRGPDDSRIVDTGNGLLGF
                     HRLAIMGLTPSGMQPFQLDGSYVVCNGEIYGFEKIKEELSDRYSFASDSDCEILLPLY
                     KEYGTDMFAMLDAEFACILYDG"
BASE COUNT          761 a          535 c          757 g          718 t
ORIGIN      
        1 attattttca atggaaacgg ttatgacgat gcatggataa aagaagcaac ggaaaagcgc
       61 ggactgatga actatccgtc aacgccggac tgtatgccgc atctgctgga tgaaaaaaat
      121 gtcaggatgc ttacttcaca taaagtattc tccaaggcgg aactggaatc aagatgcgag
      181 attatgctgg aaaactactg taagacgatc gtaatagaag caaatactat ggtggatatg
      241 gttaaaagag agatcattcc ggctgtggaa gcctacgcaa tggaactggc aaggacagct
      301 tctgcgaaga aacgtgtcga tgagacattg gcatgccgtt atgaaagcgg cctgataaaa
      361 aaactctcta cacttacaga ccagatggca gtcaggacgg atgcattgga aacagctctg
      421 atagaacttc aggacgtgaa ggatattgtg gcgcagtctt atcagatccg ggatacagta
      481 ttgaccagaa tgcaggagtt acgaattgcc tgtgatgaag cggaaaccgt tacagcaaaa
      541 aaatactggc cgttcccaac ctacggggat ctgcttttca gcgtaaaata gcaaagataa
      601 caaaaaggga ggggttatca tgtacagttc tgtaaataca atctgggtat tatttggcac
      661 agctctggtc ttctttatgc aggcaggttt ttccttgtgt gaagcgggat ttacaagagc
      721 gaaaaacacc ggaaatattc tgatgaaaaa cctgatggat ttctgcattg gaacaccggc
      781 attctggctg gtaggattcg gactgatgtt tggaaaaggt tccggaatca ttggcagctt
      841 tgacccgctg atccggggcg agtacagcca ggcgcttcct tcaggcgttc ctctgtgggc
      901 gtttgcgatc ttccagactg tcttttgtgc gacatccgcc acgattgtgt caggagcaat
      961 ggcagagaga acaaaattca gcgcttactg tatttattct gcagcgatct cacttctgat
     1021 ctatccggtt tctggccact ggatctgggg aggaggatgg ctttcccaga tgggattcca
     1081 tgattttgca ggatctacag ccgtacatat ggtaggaggc atctgcgcta tgatcggtgc
     1141 gaaaatctta ggtccgcgta ttggaaaata cgataaaaac ggaaaaccgc aggcgatttt
     1201 agggcataac cttacatttg cagcgcttgg cgtctttatc ctctggttct gctggtttgg
     1261 atttaacgga gcttctacgg taggtatgga cagtgatgcc ctgatagaaa cagcgggaag
     1321 ggtattcttt aatactaacc tggcagctgc tgtcgcctgc tgcgcggctt tgattttcac
     1381 ctggatacgt tacaagaaac cggatgtctc tatgacttac aatgcagcat tggcaggtct
     1441 ggtaggcatt acagcgggct gcgatgcggt agatgcggtc ggcgcggcgg tcattggagt
     1501 tgtctgtgga atcctgattg tactggcgat agaatttttt gataaaattg caaaaataga
     1561 cgaccctgta ggcgctgttt ccgtacactg tgtctgcggc gctgcaggaa cggttctgac
     1621 cggtctgttt gccacagggg agactacaga ggcaggactt ttctatggag gcggcgcgca
     1681 ctttctggga atccaggtat taggcgtgct tgctgtagca gcgtatgttg cggtggttat
     1741 caccattgta tttttagcaa ttaaacatac catcgggctg cgtgtaaaac cggaagaaga
     1801 gcttgccgga ttggatgttt cggaacatgg actgtttacc gcatatgccg gattttctat
     1861 gctgccggac accattgcgg aggatgtgga caccaatacg gtctttgcca tgggagatgt
     1921 gccggaagct gaagctgtac cggtgaagac ggttcctgca tttgaagaag gaacgccgaa
     1981 attcacgaag gtggaaattg tctgcaagga atcaaagctt gaggcattaa aagcggcaat
     2041 gatgaatctg ggaattacag gcatgaccgt atcgcacgtt cttggatgcg gcgtacagaa
     2101 aggaaaaccg gagtattacc gcggcgtaca gattgaggca aacctgcttc cgaaaatcca
     2161 ggtagaaatc gtggtgagca aagttcctgt ccgcagtgtt atcgaaacgg ctaagaaagt
     2221 gctttataca ggacatatcg gagacggtaa gattttcgtt tatgatgtgg aaaatgttgt
     2281 aaaaatcaga accggtgaag agggctttga tgctctgcag gatgtagaat aaatttaaaa
     2341 taagggaact gtttacagcg cgcatggaac agttcccgaa aaatggaggt aaaatgtatg
     2401 tgttcgatta tgggatattg cggaccggta gtcgatttag ataaatttaa agaaggtttc
     2461 caaagaacca tttccagagg tccggatgac agccggatcg ttgatacggg aaatggtctt
     2521 cttggatttc acagactggc cattatggga ttaacaccgt ccggaatgca gcctttccaa
     2581 ttggatggaa gttacgtggt ctgcaacgga gaaatttatg gatttgaaaa gatcaaagaa
     2641 gaattgtctg accgatatag ctttgcaagc gattcagact gtgagatcct tctgcccttg
     2701 tataaagaat atggcacaga tatgtttgct atgctggatg cagaattcgc atgtattctc
     2761 tatgacggcg a
//
LOCUS       DBMDQJ010000316         2771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12995_length_2771_cov_1.401873, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000316 DBMDQJ010000000
VERSION     DBMDQJ010000316.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12995_length_2771_cov_1.401873"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="ACZO9R_07200"
     CDS             complement(<1..430)
                     /locus_tag="ACZO9R_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR0912886.1"
                     /translation="MQELHIGENDAGQRFDKYLKKLLAEAPGSFVYKMLRKKNIILNG
                     KKADGTEKLSCGDEVKLFLSDETYEKLRAKSKTVMPEAFSGSEKTLRQLKVLYEDADI
                     LIINKPSGMLSQKARPEDVSANEYILSYLLREGALTEEEMR"
     gene            complement(452..2419)
                     /locus_tag="ACZO9R_07205"
     CDS             complement(452..2419)
                     /locus_tag="ACZO9R_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HIR0912887.1"
                     /translation="MNNGFLPVSRAEMEERGIKQFDFVYVIGDAYVDHPSFGHAIISR
                     LLEAHGYSVGIISQPDWRDKESVCIFGEPRLGFLVSAGNMDSMVNHYSVSKKRRATDA
                     FTPGGVMGKRPDYATIVYCNLLRQTYKHTPIIIGGIEASLRRLAHYDYWSNKMKRSIL
                     LDSGADLISYGMGEHSIVEIADALNSGLAVSDITFIDGTVYKTRKREDIYDAIELPHY
                     EEVLADKAVYARSFYTQYCNTDPFVAKRLFETYDGKLFVVQNPPAKPLTQSEMDQVYA
                     LPYQRTYHPSYEAQGGVPAIEEVRFSLVSNRGCYGGCSFCALTFHQGRIIQVRSHESI
                     LAEAEKIVWEPDFKGYIHDVGGPTANFRAPACDKQMTKGVCPNKQCLFPTPCKNLKVD
                     HKDYLKLLRKLRELPNVKKVFIRSGIRFDYLIYDEDRTFLRELCEHHVSGQLKVAPEH
                     ISDAVLEKMGKPRVEVYKRFVKAYQDMNEKLGKKQYLVPYLMSSHPGSTLKEAVELAE
                     FLRDLGYMPEQVQDFYPTPSTISTCMYYTGLDPRTMQPVYVAVNPHEKAMQRALIQYR
                     NPKNYDLVVEALTKAGRTDLIGFDKHCLVRPRGIGYGRTNAKDRYTGGDKNRRTAGGG
                     NNRGRANASEKKNTKKTIRNVHHKKAGAGKK"
     gene            complement(2421..>2771)
                     /locus_tag="ACZO9R_07210"
     CDS             complement(2421..>2771)
                     /locus_tag="ACZO9R_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase family protein"
                     /protein_id="HIR0912888.1"
                     /translation="VAKTYYARVQGVVDETDVNLFKNGVDIGEEKPVRPADLVILGCK
                     ADVSEITLTITEGKFHQVKRMFEAVGKKVLYLKRISMGPLVLPEDLAPGAYRKLTAEE
                     LAALKNEQPKRNED"
BASE COUNT          581 a          830 c          637 g          723 t
ORIGIN      
        1 tgcgcatctc ctcctcggtc agcgctccct ccctaagcag atagcttaag atatattcat
       61 tggcggaaac atcctcgggt ctcgccttct gggagagcat gccggacggc ttgtttatga
      121 taagaatatc cgcatcctca taaaggactt taagctgcct taaagtcttt tctgaaccac
      181 tgaaagcttc tggcatcacc gtttttgact ttgcccggag cttctcgtag gtctcgtccg
      241 agagaaacag cttcacctcg tccccgcagg agagcttctc cgtgccgtcc gcctttttgc
      301 cgtttaagat aatgtttttc ttgcgcagca tcttgtagac aaaactgccg ggagcctctg
      361 ccagaagctt ttttaaatac ttgtcaaaac gctgtcccgc gtcgttctcg ccgatgtgca
      421 attcctgcat agtctctctc cattcgcccg cttacttttt ccccgcacct gcttttttat
      481 gatgaacatt gcggattgtc tttttcgtat ttttcttctc ggaagcattt gctcttccgc
      541 ggttattccc gccgcctgcc gtgcggcggt tcttgtcacc tccggtgtag cggtctttgg
      601 cgtttgttct gccgtagccg atgccccgcg gccgtaccag acagtgtttg tcaaagccga
      661 tcagatccgt ccgccccgct ttggttaacg cctccacaac aagatcgtaa ttcttcgggt
      721 tccggtactg gatgagcgcg cgctgcatgg ctttctcgtg ggggtttacc gccacataga
      781 ccggctgcat cgtgcgcggg tcaagtcccg tatagtacat gcaggtggat atggtcgacg
      841 gggtcggata aaaatcctgt acctgctccg gcatatagcc gaggtctctt aagaattcgg
      901 cgagttccac cgcctccttc aacgtcgatc ccggatggga tgacatcaga tacggcacca
      961 gatactgctt tttcccgagc ttctcgttca tatcctgata ggccttcaca aaccgcttgt
     1021 acacctccac gcgcggtttg cccatcttct ctaagaccgc atcggaaata tgctccggcg
     1081 ccacctttag ctgaccgctg acgtggtgtt cacagagttc tctgaggaag gtgcggtcct
     1141 catcgtagat cagataatcg aaacggatac ccgaacggat gaacaccttt ttcacattcg
     1201 gcagttcccg tagctttctt aagagcttca ggtaatcctt atgatccacc tttaagttct
     1261 tgcacggtgt cggaaacagg cactgcttgt tgggacatac gccctttgtc atctgcttgt
     1321 cacatgcagg cgcgcggaaa ttggccgtcg gcccgccgac atcatggatg tatcccttaa
     1381 agtccggttc ccagacgatc ttctccgcct ccgccagaat ggactcgtgg ctgcgcacct
     1441 gaataatccg tccctgatgg aacgtaagcg cacaaaaact gcatcctccg tagcagccgc
     1501 ggttgctgac cagagaaaaa cgcacttcct cgattgcagg aaccccgccc tgcgcttcgt
     1561 aactcggatg atacgtgcgc tgatacggca gcgcatagac ctgatccatc tcgctttgtg
     1621 tcagcggctt cgccggcgga ttctgtacga caaaaagctt tccgtcgtac gtttcaaaca
     1681 gacgtttggc tacaaacgga tcggtattgc agtactgggt ataaaagctg cgcgcatata
     1741 ccgccttgtc cgccagcacc tcctcataat gcggaagctc tatggcatca tagatgtcct
     1801 cacgctttct ggtcttatag accgtgccgt cgataaatgt gatatcgctg accgccagtc
     1861 cgctgtttaa ggcatccgca atctccacaa tgctgtgttc ccccataccg taggagatca
     1921 gatccgcccc ggaatccaga agaatggacc gcttcatctt gttcgaccag taatcataat
     1981 gcgccagtct gcgcaggctg gcctcaatgc cgccgataat gatcggcgtg tgcttgtagg
     2041 tctgccggag cagattacaa tagacaatgg tcgcgtaatc cggccgtttt cccatcacgc
     2101 cgcccggcgt aaacgcatcc gtcgcgcgcc ttttcttgga caccgaatag tgattcacca
     2161 tcgaatccat attgcccgca gacactaaaa acccgagccg cggctccccg aagatacaga
     2221 cagactcctt atctctccag tccggctgcg agataattcc gaccgaatag ccgtgtgctt
     2281 ccagaagtct tgagataatg gcgtgaccga acgaaggatg atccacgtag gcatccccga
     2341 tcacatagac aaagtcaaac tgtttgattc cgcgctcttc catctccgca cggctgactg
     2401 gcaaaaatcc gttgttcatt ctaatcctca tttctcttcg gctgctcatt ctttaacgca
     2461 gcaagctctt ccgcggtcag tttccggtac gctcccggtg caagatcctc cggcagtacc
     2521 aatggtccca tggaaatccg cttcaaatac agtacctttt tcccgaccgc ctcaaacatc
     2581 cgctttacct gatggaactt tccctccgtg atggtaagcg taatctctga cacatccgct
     2641 ttacagccca gaattaccag atctgccggt ctgaccggct tctcctcccc gatgtcaacc
     2701 ccgtttttaa aaaggttgac atcggtctca tccaccacgc cctgcacccg ggcgtaatat
     2761 gtttttgcca c
//
LOCUS       DBMDQJ010000317         2771 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_12998_length_2771_cov_1.283146, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000317 DBMDQJ010000000
VERSION     DBMDQJ010000317.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12998_length_2771_cov_1.283146"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            110..832
                     /locus_tag="ACZO9R_07215"
     CDS             110..832
                     /locus_tag="ACZO9R_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HIR0912889.1"
                     /translation="METGRKIPQHIAIILDGNGRWAKAKGMPRNYGHTAGAKNVETVC
                     QAAHDLGVKYVTMYAFSTENWNRPEGEVEALMKLLESYLKNCIKTADKNNMRVRVIGD
                     TTRLSERFQERIRELEAASAKNDGLNLQIAINYGSRDEMTRAMRRMSEDVAAGKRKPE
                     EITESVFEEYLDTAGIPDPDLLIRTSGELRLSNFLLWQLAYSEFYFTDVPWPDFHKEE
                     LERAIEAYNKRDRRFGGLTDKK"
     gene            857..1663
                     /locus_tag="ACZO9R_07220"
     CDS             857..1663
                     /locus_tag="ACZO9R_07220"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR0912890.1"
                     /translation="MFKTRLLSGIVLVIAALVLIITGGDVLLVSTLLISWIGMFELYR
                     IFHIEKSAPGIVGYAAAAVYYANLRFGFLPDLMMLVLGLLIVLMFVYVFTYPKYQTEQ
                     LLAAFFGVFYVAVMLSYVYQTRMLSAGTYIVWLIFLCSWGCDTCAYCVGMLIGKHKMT
                     PKLSPKKSVEGAVGGVIGAALLTVIYGMVFRGAMGSEMSHVWIMAAISAAGALVSMVG
                     DLTASAIKRNYGIKDYGKLIPGHGGILDRFDSVIFTAPLIYFLAANLIVL"
     gene            1679..>2771
                     /locus_tag="ACZO9R_07225"
     CDS             1679..>2771
                     /locus_tag="ACZO9R_07225"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR0912891.1"
                     /translation="MMKIAILGSTGSIGTQTLDIVREQGDIQVTAIAAGSNIKKLEEQ
                     MREFHPLLVCVFQKDAAKELRDRTKDLNIRVVSGMDGLLEVAVQPESEILVTAIVGML
                     GIRPTIAAIEAGKKIALANKETLVTAGHIIMPLAKKCGVPILPVDSEHSAIFQSLQGE
                     NGNRIAKILLTASGGPFRGKKPEDLAHIQVGDALRHPNWTMGRKITIDSSTLVNKGLE
                     VMEAKWLFDVELEQIEVVVHPQSVIHSAVQYEDGAVIAQLGTPDMRLPIQYALYYPKR
                     RPLSGDRLDLFALGQLTFERPDTETFRGLALAYQAMRRGGNIPTAFNAANEKAVALFL
                     DRKIAYQQIPEMIESCMEAADFIANPTVDE"
BASE COUNT          671 a          591 c          856 g          653 t
ORIGIN      
        1 gatttgaaac gagaggagat acaggtgttt atggaggcat acatctgtat cttttccgtt
       61 attttgtata gttgatactt cagtacagat acgagggaga agcacaggta tggagacggg
      121 aagaaagatt ccacagcata tagcgatcat actggatgga aacggaagat gggcaaaggc
      181 aaagggaatg ccccggaatt acggacacac agcgggagcc aagaatgtcg agacggtatg
      241 ccaggcggca catgatctcg gtgtaaagta tgttaccatg tacgcatttt cgacggaaaa
      301 ctggaaccgc ccggagggcg aagtggaagc gctgatgaag cttttggaaa gctatctgaa
      361 gaactgcatt aagacagcag acaaaaacaa tatgcgcgtg cgcgtgatcg gcgatacgac
      421 gagactcagt gagcgctttc aggagcgcat ccgtgaactg gaggctgcct ccgcaaaaaa
      481 tgacggcttg aatcttcaga ttgccatcaa ttacggcagc cgcgacgaga tgacacgcgc
      541 aatgcgccgt atgagcgaag atgttgcggc ggggaagaga aagccggaag agatcacgga
      601 aagtgtgttt gaggagtatc tggataccgc aggcattccg gatccggatc ttttaatccg
      661 cacgagcggg gaactcaggt tgtccaattt cctgctctgg cagctggcgt acagtgaatt
      721 ttactttacc gatgtgccgt ggccggattt tcacaaagag gaattagagc gtgccattga
      781 agcgtataat aaaagagaca gaagatttgg cggtttgaca gacaagaaat aggaaggact
      841 gccggatgga ggagatatgt ttaagacgag attattgagc ggaatcgttc ttgtgattgc
      901 cgcattggta ttgattatta ccggcgggga tgtgctgctg gtgtccacgt tgctgatttc
      961 ctggatcggg atgtttgaat tataccggat ttttcatatt gagaagtctg caccgggcat
     1021 tgtcgggtat gcggctgcgg cggtctacta tgcgaatctg cgcttcggct ttctgccgga
     1081 tctgatgatg ctggttttag ggcttctgat cgtattgatg ttcgtgtatg tgttcactta
     1141 cccgaagtat cagacggagc agctgctcgc cgcatttttc ggcgtgttct atgtggcagt
     1201 gatgttgtcc tacgtgtacc agaccagaat gcttagcgcc ggaacctaca ttgtgtggct
     1261 tatttttctg tgttcctggg gatgcgacac ctgcgcgtac tgtgtcggca tgctgatcgg
     1321 gaagcataag atgacgccga agctgagtcc gaaaaagtcc gtggagggcg ccgtgggtgg
     1381 tgtgatcgga gctgcgcttc tcaccgtgat ttacggcatg gtatttcgcg gggcgatggg
     1441 aagtgagatg tcacatgtct ggattatggc agccatcagt gcggcgggcg cactggtatc
     1501 catggtggga gatctgacag cttccgccat caagcgtaat tacggcatca aggattatgg
     1561 aaaactcatt ccgggacacg gcggtatcct ggatcgtttc gacagcgtca tttttacggc
     1621 gccgctcatc tatttccttg ccgctaattt gattgtattg taattttggg agagacctat
     1681 gatgaagata gctattttgg gatcgacagg ctccattggg acacagacgt tagatattgt
     1741 gcgggagcag ggcgacatac aggtgacggc gattgcggcc ggaagcaaca tcaaaaagct
     1801 ggaagagcag atgagagagt ttcatccgct gctggtatgc gtttttcaga aggacgccgc
     1861 caaagaactg cgcgatcgca cgaaggatct gaatatccgc gtggtgtccg gcatggatgg
     1921 acttctggag gtggcggtac agccggagag tgagatcctt gtgacggcga ttgtcgggat
     1981 gctcggcata agaccgacga ttgcagccat tgaagcgggg aaaaagattg cacttgcgaa
     2041 caaggagacg cttgtgacgg caggtcatat cattatgccg ctggcaaaaa aatgcggcgt
     2101 gccgattctg ccggtcgaca gcgagcacag tgctattttc cagtcgctgc agggagaaaa
     2161 cgggaaccgc attgcgaaga ttcttctcac ggcgtccggc ggaccgttcc gcgggaaaaa
     2221 gccggaagat ctggcgcaca ttcaggtggg ggatgcgctg cgtcatccga actggacaat
     2281 gggacgcaag atcacgatcg attcgtcaac gcttgtcaat aaaggcctgg aagtcatgga
     2341 ggcaaagtgg ctgtttgatg tggagctgga gcagattgag gtggttgtcc atccgcagag
     2401 tgtgatccac tccgcggtgc agtatgagga cggcgcagtc attgcgcagc tcggcacacc
     2461 ggatatgcgt ctgccgatcc agtatgcgct ctattatccg aagcgcagac cgctctcggg
     2521 cgacagactg gatctgtttg cattgggaca gctgacgttt gagagaccgg acacggagac
     2581 gttccgcggt cttgcgttag cctatcaggc gatgcgccgc ggcggcaata ttccgacggc
     2641 atttaatgcg gcgaatgaaa aggcggtagc gcttttcctg gatcgaaaaa ttgcctatca
     2701 gcagatcccg gagatgattg aatcgtgtat ggaggcagct gattttattg ccaatccgac
     2761 ggttgatgag a
//
LOCUS       DBMDQJ010000318         2753 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13096_length_2753_cov_1.304299, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000318 DBMDQJ010000000
VERSION     DBMDQJ010000318.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13096_length_2753_cov_1.304299"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(74..820)
                     /locus_tag="ACZO9R_07230"
     CDS             complement(74..820)
                     /locus_tag="ACZO9R_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR0912892.1"
                     /translation="MIREQRKKKRRRKIGLTASLILLMLLAIAFLIVWKVFTVENVVV
                     EGNELYSSEQIEQLVLNDEYSWNSLYVLLKYRIRDTGKVPFVDTMEVTLDDPHTVHVL
                     VYEKGILGCFYIDAIGQYAYFDKDGFVVETSSDEIEDVPKITGVTCDEVVLYEKLPLD
                     QPDVLNNLLSLTQILKKYDLLPQEIHYDSSLKPTLTYYGIQVSIGSDDYLSQKIVRLS
                     AILPQLAGMYGTLHLDTWTPDTTDIVFDRD"
     gene            complement(824..1942)
                     /gene="ftsW"
                     /locus_tag="ACZO9R_07235"
     CDS             complement(824..1942)
                     /gene="ftsW"
                     /locus_tag="ACZO9R_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR0912893.1"
                     /translation="MARRQEKEKKQKPIRYFDYSLLFLIIFLLCFGLVMLYSTSSYYG
                     STRFGDTAYYLKKQLFATSLGIIAMIIVSRIPYRFWMRVSGLAYLMAFGLCTAVIFIG
                     TEAKGQARWIYIGPLSFQPSEFAKLAVIIFLATIIYKTSKQVGDFKTLVKVMAIVLPV
                     VGVVAYNNLSTAIIILGIAVCMLFVASPKYSHFLIVGGVVLAVGVIFIALASYRAERI
                     QIWLHPEEYEKGYQTLQGLYAIGSGGLFGKGLGESMQKLGFIPEAQNDMIFSVICEEL
                     GLFGAICLILLYLLIIWRFMVIANNAADLYGALLVVGIMAHISIQVVLNIAVVTNTIP
                     NTGISLPFVSYGGTSILFLLSEMGLALSVSRGIKFEVT"
     gene            complement(1952..>2753)
                     /gene="murD"
                     /locus_tag="ACZO9R_07240"
     CDS             complement(1952..>2753)
                     /gene="murD"
                     /locus_tag="ACZO9R_07240"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005331625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR0912894.1"
                     /translation="PDHLNRHHTMEAYIAAKENIARNQTEDDYCVLNYEEPVMRKFAE
                     TLKCHVLFFSSQHKLEKGIYLDNDKIIYKNPDEVEVCHVDELQILGTHNYENVMAAVC
                     MAAVYGVPMETIRKAILAFKGVAHRIEYVAEKNGVVYYNDSKGTNPDAAIKAIQAMRR
                     PTILLGGGYDKDSEYTEWINSFDGKVKKLILMGATKEKIARDCEKCDFHDYVYVDTFE
                     EAMNLAVEMAEPGDAVLLSPACASWGMFKNYEERGRIFKEYVRALKED"
BASE COUNT          735 a          743 c          541 g          734 t
ORIGIN      
        1 atacaatata atatattctt tctttcaatt tatcaactaa ttttttcact tttttgaaca
       61 aattaaggat tttctaatct ctgtcaaaca caatatctgt ggtgtccggt gtccatgtgt
      121 ccagatgaag tgttccgtac attcccgcaa gctgtggaag gatcgccgac agacgcacaa
      181 tcttctgcga cagatagtcg tccgaaccga tcgaaacctg tatgccatag taggtcagcg
      241 tcggttttaa gctgctgtcg tagtgaatct cctgcggcag aaggtcgtat tttttcaaaa
      301 tctgcgtcag gctcagaagg ttgtttagca catccggctg atccagcggc agtttctcgt
      361 agagcacgac ctcatcgcat gtcactccgg tgatctttgg cacatcctca atctcgtccg
      421 aagaagtctc cacgacgaat ccatccttgt caaaataggc gtactgcccg atggcgtcaa
      481 tgtaaaagca gcccagaatt cctttttcgt ataccaggac atgcaccgtg tgcggatcat
      541 ccagcgtcac ttccatcgtg tcgacaaaag gcaccttgcc ggtatccctg atgcggtatt
      601 tcagaagaac atacagagaa ttccacgaat actcatcatt taacaccagc tgctcgatct
      661 gttcggaaga atagagttca ttcccctcca ccacgacgtt ttccaccgta aatactttcc
      721 agacaatcag aaatgcaatc gctagaagca tcagaaggat cagtgaagcc gtcagtccga
      781 ttttccgtct tcttttcttt tttctctgtt ctctaatcat actttaggta acctcaaact
      841 ttattcccct tgacacagac agcgcaagcc ccatttccga gagcagaaac agaatggagg
      901 ttccaccgta actgacaaac ggaagggaaa ttccggtatt cggaatggtg ttggtcacaa
      961 cggcaatatt taaaaccacc tggatggaaa tatgtgccat aatgccgacc acaagcaacg
     1021 ccccgtagag atccgccgca ttgttcgcaa tcaccataaa tctccagata atcagcaagt
     1081 ataaaaggat cagacagatc gcgccgaaaa gtcccagctc ctcacagatg acggagaaga
     1141 tcatatcgtt ctgcgcctcc gggataaaac cgagcttctg catgctctcc ccgagtccct
     1201 tgccgaacag cccgccggaa ccgatcgcat agagtccctg cagcgtctga taaccttttt
     1261 catactcttc cgggtgcagc cagatctgga tacgctcggc gcggtaggat gcaagcgcga
     1321 taaaaataac gccgactgcg agtaccacgc ctcccacaat caggaaatgc gaatatttcg
     1381 ggctcgccac aaacagcatg cacaccgcga tgccaaggat aataatcgct gtacttaagt
     1441 tattgtatgc gaccaccccg acgacgggaa gcacgatcgc catgaccttg accagcgtct
     1501 taaaatcccc cacctgcttc gacgtcttat agatgatcgt cgccaggaaa atgatgaccg
     1561 caagcttcgc aaactcggat ggctggaacg aaagcggtcc gatgtagatc cagcgcgcct
     1621 gtcctttggc ttccgtaccg ataaagataa ccgccgtaca cagtccgaac gccatcagat
     1681 acgccagacc gctcactctc atccagaatc ggtacgggat tctcgagacg atgatcatcg
     1741 ctatgattcc aaggcttgtc gcaaacagct gctttttcag ataatacgcc gtatccccga
     1801 aacgcgtgga gccatagtaa gagctcgtgc tgtagagcat caccagaccg aaacaaagca
     1861 gaaagataat caaaaacagc aggctgtaat caaaatatcg aattggtttt tgtttttttt
     1921 ctttttcctg ccttcgcgcc attctccact cctagtcttc ttttaaggca cgcacatatt
     1981 ccttgaaaat gcgtccgcgc tcctcatagt ttttaaacat tccccagctt gcacacgccg
     2041 gagacagaag cactgcatct cccggctctg ccatctccac tgcaagattc atagcttctt
     2101 caaatgtgtc cacatacaca taatcatgaa aatcacattt ttcacagtct ctcgcaatct
     2161 tttcttttgt cgcacccatc aggatcagtt tcttgacttt tccatcaaaa ctgttaatcc
     2221 actctgtata ctcggaatcc ttgtcgtatc ccccgcctaa caggattgtc ggccggcgca
     2281 ttgcctggat tgccttaatt gcggcatccg gattcgttcc tttagaatca ttgtaataaa
     2341 caactccgtt tttctcagcc acatattcta tacgatgtgc gactccttta aatgcaagaa
     2401 ttgccttcct gattgtctcc atcggaacgc cataaacagc tgccatgcat acagctgcca
     2461 taacattttc ataattgtgc gttcctaaaa tctgaagttc gtcaacatgg cagacttcga
     2521 cttcatccgg attcttgtaa ataattttat cgttatccag ataaatacct ttttccagct
     2581 tatgctgact gctgaagaac agaacatgac actttaatgt ctctgcgaat ttacgcataa
     2641 ccggctcttc atagttcaga acacaataat cgtcttctgt ctgatttctt gcaatgttct
     2701 ccttagcagc aatataagct tccatcgtat gatgccggtt caaatgatcc ggt
//
LOCUS       DBMDQJ010000319         2751 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13103_length_2751_cov_1.887925, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000319 DBMDQJ010000000
VERSION     DBMDQJ010000319.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13103_length_2751_cov_1.887925"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            401..2089
                     /locus_tag="ACZO9R_07245"
     CDS             401..2089
                     /locus_tag="ACZO9R_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0912895.1"
                     /translation="MKEEQKVSFIHSISAKIMLLTVLAVVMSVLIVAFRAEAGAKKVV
                     GDVSADYILSMAEMGVEIVNNLPQEADAGAYENALADISMEGIDSSYAYLVDTDGTML
                     YHPTAEKIGQPVENAVVLGVVAELAKGQVPVNETVTYDFKGVTKYAAYAITANRQIVV
                     VTADEDEIMKPLNQIVSKIMLLSLGILAAAIVLAYVVSQFICRPIQQITTIIVDTAQL
                     DFRSNPNGAKLRSRRDETGEMANAVHVMRRNLRSMIGDIDTASNQITQNVDGLQEITT
                     TVDHMCSDNSATSQQLAAGMEETAATTVTINENIEVIKNGADDINSMATEGARTSEAI
                     MMRANDLRTKTVEASTKTMTMYNNVKTKAQEAIEGSKAVDKINELTGTIMEISSQTGL
                     LALNASIEAARAGEAGRGFAVVATEIGSLADQTSKAIKDIGTIVDAVNAAVSNMAECL
                     EETTGFLENTVLTEYKEFEQVSEQYQEDADTFKTSMNDVSDAMAGLANSIDAIAQALS
                     GINSTVGESSIGVSDIAEKTSDMVEKTGTTHDMVEECYTYVEKLREIVSQFVLQ"
     gene            2261..>2751
                     /locus_tag="ACZO9R_07250"
     CDS             2261..>2751
                     /locus_tag="ACZO9R_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type ATPase actuator domain-containing
                     protein"
                     /protein_id="HIR0912896.1"
                     /translation="MGAAHMQEYYRGKTEERGRNVLEDEEKKSWAESFLEQLNDPLIF
                     ILFVAAAISLLLREYGDMAIILAVVLLNATVGVIQEGKAKKSLEVLKQMTSPHALLLE
                     GDEVRQIPAADLIPGDLVVLEAGCQVPADLVLTEAVNLKIEESALTGESVPVNKDTAQ
                     NRMA"
BASE COUNT          749 a          571 c          821 g          610 t
ORIGIN      
        1 gcaacgagtt tcggttcgcg cggttttata ttgtctgtga agaggtgcca ttagggacac
       61 cagaaagtcc gctcattgtc tgcggcttgt cggacgcact ccgtgagtgt ccaaacgtgg
      121 ccaagacacg gaatgggatg ccagccacgg catggaagaa atacgtgaat tttgcttcaa
      181 agaatttccc gccgtctgtt tattctggaa cttttttgtt ctctggtctt ctaataatat
      241 tacttatcat cccaaagcgg cctatgctga ttgcaacaat ttcttttgca atattttgta
      301 gaagaagagt ttctttcggc tgtatgttgt ggtaaaataa cagatataag ctgggagtaa
      361 aatttcccgg caattacgaa ggggaatgga tagaagtacc atgaaagagg agcagaaagt
      421 atcgtttatt cactcaattt cagcaaagat catgctgctg accgtgcttg cagtggttat
      481 gtcggtgttg atcgtcgcat tccgggcgga agcgggagca aagaaggttg tcggagacgt
      541 cagtgcagat tatattttaa gcatggcgga gatgggagtg gagattgtga acaatcttcc
      601 acaggaggcg gatgcaggag catatgagaa tgccttagcg gatatcagta tggagggaat
      661 tgattcttcc tacgcctatc ttgttgacac ggacggcacc atgttatatc acccgaccgc
      721 ggaaaagatc ggacagccgg tggagaatgc tgtcgtgctt ggcgtcgttg cagagcttgc
      781 caagggacag gtaccggtga atgagacagt gacgtatgat tttaagggcg ttaccaaata
      841 tgcggcttac gcgatcacgg cgaaccgtca gattgttgtg gtgacggcgg atgaagacga
      901 gatcatgaag ccgctgaatc agatagtaag taagattatg ctgctgtcat tggggatact
      961 ggctgcagcg attgttctgg catacgtagt aagccagttt atctgcagac cgattcagca
     1021 gattacaacg attattgtcg ataccgcaca gttggatttc agatcaaatc cgaacggcgc
     1081 aaagctccgc agccgccggg atgagacggg cgagatggca aatgcggtac atgtgatgcg
     1141 caggaacctg cgttccatga ttggcgatat tgataccgca agtaaccaga tcacacagaa
     1201 tgtggatggt ctgcaggaga ttaccaccac ggtggatcac atgtgttccg acaactccgc
     1261 gacaagccag cagcttgcgg caggcatgga ggagacggcg gcaaccacag taacaatcaa
     1321 cgaaaatatc gaggttatca agaacggagc ggatgatatt aacagtatgg cgacggaggg
     1381 tgcacggacc tcggaagcga tcatgatgcg ggcaaatgat ctacgcacca agacagttga
     1441 ggcaagcacc aagacaatga cgatgtacaa caacgtcaag acaaaggctc aggaagccat
     1501 cgagggatcg aaggcggtcg ataagatcaa cgaactgacc ggcacgatca tggagatctc
     1561 atcccagacc ggactgcttg cactcaatgc ttccatcgaa gcggcaagag ccggggaggc
     1621 aggacgtgga tttgcggtgg ttgcaacgga gatcggaagc cttgcggatc agacgtccaa
     1681 ggcgatcaag gatatcggca cgattgtgga tgctgtcaac gcagcagttt ccaatatggc
     1741 agaatgctta gaagagacca ccggatttct ggagaatacc gtgctgacgg agtataagga
     1801 atttgagcag gtcagcgaac agtaccagga ggatgcggat accttcaaga ccagtatgaa
     1861 cgacgtgagc gatgcgatgg cgggtcttgc caattctatc gacgcgattg cacaggcact
     1921 tagcggaatc aactccacag tgggcgagtc gtcgatcggc gtcagcgata ttgcggagaa
     1981 gaccagcgac atggtggaga agacaggaac cacgcacgac atggtggaag agtgttatac
     2041 ttatgtggag aagctgcgtg agattgtgag ccagtttgta ttacagtaaa taaaatatga
     2101 tttgcaggga aaagggtatg cagatgttgg agcgatgctg tgcatcggaa tcacgtcggc
     2161 ataccctttt tcacgcagac aggcataaac ccgtccacat tttcatagga tgagttaacg
     2221 gcaaagcggt ggacgtgctg ctttgtggga aactcgatgg atgggggcag cgcatatgca
     2281 ggaatattat cgcggaaaaa cggaagaacg agggagaaac gtgctcgagg atgaggagaa
     2341 aaaatcctgg gcggaaagtt ttctggaaca gttaaacgat cctctgatct ttatcctgtt
     2401 tgtggcggcg gcgatctccc tgctgctgcg ggaatacggc gatatggcaa ttattctggc
     2461 tgtggtgctg ttaaatgcga cggtgggagt gatacaggag ggaaaagcga aaaaatcgct
     2521 ggaggtgtta aaacagatga ccagcccgca tgcactgctt ctggaagggg atgaagtgcg
     2581 gcagattccg gctgccgatc tgatcccggg cgatcttgtg gtgctggaag ccggctgtca
     2641 ggttccggcg gatcttgtgc tgacagaagc ggtgaacctg aaaatagaag aatccgcact
     2701 gaccggcgag tctgttccgg tcaacaagga tacggcgcag aaccgcatgg c
//
LOCUS       DBMDQJ010000320         2736 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13188_length_2736_cov_1.436053, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000320 DBMDQJ010000000
VERSION     DBMDQJ010000320.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13188_length_2736_cov_1.436053"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="ACZO9R_07255"
     CDS             <1..513
                     /locus_tag="ACZO9R_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HIR0912897.1"
                     /translation="KNIFILPNNKNIILAANQAASLVEDKKIIVIPTKTIPQGITALI
                     NYIPDSTPEENAERMSEELGTVKTGQVTYAVRDTVIDDKEIKQDDFMGIGDQGILAVG
                     KELEATVLDMIEQLIDEDSAIVSIYYGEDAREDAANAIGEKITEAHPDVEVEVHYGGQ
                     PIYYYVISVE"
     gene            614..2671
                     /gene="recG"
                     /locus_tag="ACZO9R_07260"
     CDS             614..2671
                     /gene="recG"
                     /locus_tag="ACZO9R_07260"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR0912898.1"
                     /translation="MNQSTSIIEIKGIGEKTAALFQKLNIETVGDLLLHYPRTYVQFP
                     VAKEVAEVTDGETAAVLGRVRKTPVVRRTRSMPVTVTTIGTDEAELELVWFRMPYIKS
                     QLAPGNTYVFYGKVARKGSRLVMEQAAIYSGEAYAAMEQAFLPVYGLTGGISNNLVTK
                     TVRSVLGREELFKEYLPREIRSRYKLCEYNYAIKEIHFPENMDTLIAARNRLVFDEFF
                     LFILNMQYHKEKRSKEANEFEFREDSFTDELIARLPYELTGAQKRALSEVKRDMRSPY
                     VMQRLIQGDVGSGKTIIAFLAMADAAHNDYQSAIMAPTEVLARQHYETFQKLCEEFGL
                     KIPVVLLTGSMTARQKRMAYEALQLYPNAMVVGTHALIQERAIYDNLALVITDEQHRF
                     GVRQREIFAEKGRHPHILVMSATPIPRTLAIILYGDLDISVVDEVPAKRLPVKNCVVN
                     TGYRNKAYAFIENEIKNGHQAYVICPLVEEVEGMETENATDYVKKLRQIFPEEIQLGL
                     LHGQMKPAQKNKVMEAFMKNEVQVLVATTVVEVGVNVPNATVMMIENAERFGLAQLHQ
                     LRGRVGRGDAQSYCIMVNCSSAKTAERRLKILNESNDGFRIASEDLKLRGPGDFFGIR
                     QSGELQFALGDIYQDAAVLRDASEAVGELLGEDPDLMSGEHENLRHYLEVFLMREQNQ
                     MSL"
BASE COUNT          702 a          627 c          834 g          573 t
ORIGIN      
        1 aagaatatct ttattcttcc gaataacaag aatattattc ttgcagccaa tcaggcagcc
       61 tctcttgtgg aggacaaaaa gatcatcgtg atcccgacca agacgatccc gcagggaatc
      121 acggcactta tcaactatat tccggacagc acaccggagg agaatgcaga gcgcatgagc
      181 gaggagcttg gcacggtaaa gaccggtcag gtgacctatg cggtgcgcga taccgtgatc
      241 gatgacaagg agatcaagca ggatgatttt atgggcatcg gcgatcaggg aatccttgcc
      301 gttgggaagg agcttgaggc tacggtgttg gatatgatcg aacagctcat cgatgaggat
      361 tcggcgattg tcagcatcta ctacggggag gatgccaggg aagatgcagc gaatgcgatc
      421 ggtgaaaaga tcacagaggc gcatccggat gtcgaggtgg aagtccacta tggcggacag
      481 ccgatctatt attacgtgat ctccgtcgag taagacgaaa agtgtaaata acataagcac
      541 aggcagggct gctgcacgga catcgcttga ggcggttgac ggtgcggcag cctttttcta
      601 aaggaaacag agtatgaatc agagtacatc cataatagag atcaagggaa tcggggaaaa
      661 gacagccgcc ctgttccaga aattaaatat cgaaacggtg ggggatctgc tgcttcatta
      721 tccgaggacg tatgtgcagt tcccggtagc aaaagaggtg gcggaggtca cggacggcga
      781 gacggcggcg gtactcggca gggtgagaaa gacacccgtt gtgagacgca cgagaagcat
      841 gccggtcacg gtgacgacca tcggcacgga tgaggcggaa cttgaactgg tgtggtttcg
      901 gatgccatac atcaaaagcc agcttgcacc gggcaatacc tatgtatttt acgggaaggt
      961 ggcgcgcaaa ggcagccgtc ttgtgatgga gcaggcagcc atctattccg gggaagcgta
     1021 cgccgccatg gagcaggcat ttttgccggt gtatggtctg actggaggaa tctcgaacaa
     1081 tctggtcaca aagaccgtgc gctcggtgct cggcagagaa gaactgttta aggaatatct
     1141 gccccgggag atccgcagcc gctacaaact ctgcgagtac aattatgcga tcaaagagat
     1201 tcattttccg gaaaatatgg acacgctgat tgcagcgcgc aaccgtctcg tgttcgatga
     1261 gtttttcctc tttattttaa atatgcagta ccacaaggag aagcggagca aggaagccaa
     1321 cgaatttgaa ttccgggagg attcctttac cgatgagctg attgccaggc tgccgtatga
     1381 gctgaccggc gcgcagaagc gggcgctctc ggaagtgaag cgcgatatgc gaagccctta
     1441 tgtcatgcag cggctgattc agggagatgt cggttccgga aagacgatca tagcattttt
     1501 agcgatggcg gatgccgcgc acaatgatta ccagtcggcg atcatggcac cgacggaagt
     1561 gcttgccaga cagcactatg agacgtttca gaagctgtgc gaagagtttg gattaaagat
     1621 tccggtggta cttctcaccg gttccatgac ggcgcggcag aagcgcatgg catacgaggc
     1681 actgcagctt tatccaaatg cgatggtggt cggaacgcac gcactgatcc aggagcgcgc
     1741 catctacgat aacctggcgc tcgtcatcac agacgagcag caccgtttcg gcgtccgcca
     1801 gcgggaaata tttgcggaaa agggcagaca tccgcacatt ctggtgatga gtgccacgcc
     1861 gatccccaga acactcgcca taatcctgta cggggatctg gacatctcgg tggtggatga
     1921 ggtgccggca aagcgtcttc cggtcaaaaa ctgtgtggtg aacaccggct atcggaacaa
     1981 ggcgtatgca tttatcgaaa acgagatcaa aaatggacat caggcgtatg tgatctgtcc
     2041 tctggtggag gaagtcgagg ggatggagac ggagaatgcc acggactatg tcaaaaaact
     2101 ccggcagatt ttcccggagg agatccagct cggactgctg cacggtcaga tgaagccggc
     2161 gcagaagaac aaagtcatgg aggcatttat gaaaaatgag gtgcaggtgc tggtggcgac
     2221 cacggtcgtg gaagtcggcg tcaatgtgcc gaatgccacg gtcatgatga tcgagaacgc
     2281 ggagcggttc ggactggcgc agcttcatca gctgcgcggc cgtgtgggaa gaggcgatgc
     2341 gcagtcctac tgtattatgg tgaactgctc gagcgcaaag acggcggagc gcaggctaaa
     2401 gattttaaat gaatccaatg acggattccg gattgcaagc gaggacttaa aactgcgggg
     2461 gccgggggat ttcttcggga tccgtcagtc cggcgaactg cagtttgcac ttggcgacat
     2521 ctatcaggat gctgccgtgc tgcgggatgc atcggaggcg gtcggcgaac tgctcgggga
     2581 agacccggat cttatgtccg gtgagcatga gaatctgcgg cattatctcg aagttttcct
     2641 tatgcgggaa cagaatcaga tgagcttata atttcacaca taaaacggga ccggtgcaag
     2701 cgcccgtccc gtctttttct ttggtatttt tttctc
//
LOCUS       DBMDQJ010000321         2725 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13235_length_2725_cov_1.381098, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000321 DBMDQJ010000000
VERSION     DBMDQJ010000321.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13235_length_2725_cov_1.381098"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>369
                     /locus_tag="ACZO9R_07265"
     CDS             <1..>369
                     /locus_tag="ACZO9R_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912899.1"
                     /translation="FKEHRALDHVTFDVKKGETVGIIGTNGSGKSTILKIITGVLSPT
                     SGEVEIDGRISALLELGAGFNMEYTGIENVYLNGMMMGFSREEMDARLDDILKFADIG
                     EFVNQPCKTYSSGMFVRLAF"
     assembly_gap    370..379
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <380..1230
                     /locus_tag="ACZO9R_07270"
     CDS             <380..1230
                     /locus_tag="ACZO9R_07270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015550319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912900.1"
                     /translation="PVRTYSSGMKARLGFSICVNINPEILIVDEALSVGDVFFQAKCY
                     KKFEDFKAEGKTIIFVSHDISSVAKYCDRVILLNKGVKLSEGNPKDTINLYKKVLLNQ
                     SQNIASGQVLADAKENGTKKLWKSNYELNPQVNEYGTGEAEIIDFAVIDEYGSYTSCI
                     QKGTSFTIRSKVQFHTDIQDPIFTYTFKTVQGTAVTGTNTMYEKVGVGLARAGEIYVA
                     SFKQNMDLQGGEYLLSISCTGYVGGEFRAYHRLYDVVGVTVISDKNTVGFYDMNSETT
                     VERVEE"
     gene            1286..2122
                     /locus_tag="ACZO9R_07275"
     CDS             1286..2122
                     /locus_tag="ACZO9R_07275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR0912901.1"
                     /translation="MEDAEVLKELYETIKQNSKADYAGIIEQRASYPYLYHLSQIREN
                     LVSFLPFDKDMRVLECNPECGALTGKLLAMAGGVTALAESELQEKILRERFKEPQERE
                     KLDIRRQLPEHGRFDVILIAGHFYRWEKQLPALRELLAPGGKFYVADANRIGLKYLAG
                     CQEEYCGGYFTGIDGYPDAAAVGRFGRSYSRAEYARLLQEAGFGGLTFYYPYPDHKFP
                     SVIYSDEWLPQKGELAEGRSNYDRDRVACFNERVMFDSLLEEGVFQTFSNSFLIEAVQ
                     EG"
     assembly_gap    2142..2151
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2152..>2725
                     /locus_tag="ACZO9R_07280"
     CDS             <2152..>2725
                     /locus_tag="ACZO9R_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR0912902.1"
                     /translation="YSKYSNERAERFRIRTDIVTDEAGEKKVYKYACTIQAGDHIRRQ
                     EELGKQLDAAYAGSRITFCPCTTEDVPGGCRSVSPFVQGDNLQHLMEQAVAAGDWETV
                     EQMVAAYADRVFGSGGEIPFDRTPEFAEVFGEGKLPEGIPCAAVSDVDMIFSNIFVES
                     GKAAADSAWIVIDYEWTFPFPVPKKYLIYRA"
BASE COUNT          686 a          584 c          854 g          581 t
ORIGIN      
        1 gattcaagga acatcgggca ctcgaccatg tcacgttcga tgtgaaaaaa ggtgagacgg
       61 tcggaatcat cggaacgaac ggctccggca aatccaccat cttaaaaatt atcacaggcg
      121 tgctttcccc cacaagcggt gaggtagaga tcgacggaag aatctcggcg ctgctggagc
      181 ttggcgcggg ctttaacatg gaatacaccg gcatcgaaaa cgtatatctc aacggcatga
      241 tgatgggatt ctccagggag gagatggatg caagactgga cgatattttg aaatttgcgg
      301 atatcggcga atttgtaaac cagccgtgca agacgtattc cagcggcatg tttgtgcgtc
      361 tggcgtttgn nnnnnnnnna gcctgtgcgc acgtactcca gcggtatgaa agcgcgcctc
      421 ggcttttcca tctgcgtcaa catcaatccg gaaatcctca tcgtcgatga ggcgctgtcg
      481 gtcggagacg tgtttttcca ggcaaaatgt tataaaaagt ttgaggattt caaggcggag
      541 ggcaagacga ttatttttgt cagccacgac atcagcagtg tggcaaaata ctgtgaccgc
      601 gtgattcttt taaacaaggg cgtcaagctg tcggagggaa atcccaagga cacgatcaac
      661 ctgtacaaga aagtgctgtt aaatcagtcg cagaatattg cgagcggaca ggtgcttgcg
      721 gacgcgaagg aaaatgggac aaagaagctg tggaaatcca actatgagtt gaatccgcag
      781 gtgaatgaat acggaaccgg ggaagcggaa atcattgatt ttgcggtgat tgacgagtac
      841 ggaagctata cgagctgtat ccagaagggg acgtctttta ctatccgctc aaaggtgcag
      901 tttcacaccg atattcagga tccgattttt acctatacgt ttaagaccgt gcagggaacc
      961 gctgtcacgg gaacgaacac catgtacgag aaggtcggcg tcggactggc acgggcggga
     1021 gagatctacg tggcctcctt caaacagaat atggatctgc agggaggcga gtatctgctg
     1081 tccatcagct gtaccggcta cgtgggaggc gagttccggg cgtaccaccg gctgtatgat
     1141 gtggtgggcg ttacggttat atcggacaag aatacggttg gtttctatga tatgaattcg
     1201 gagacgaccg tggagcgtgt ggaggaataa aagagcaggg aacaccgaca gcatgtcaga
     1261 cagcagaggg atgcggagga acagtatgga ggatgcagaa gttctgaagg aactgtatga
     1321 gaccataaaa cagaatagta aggcagatta tgcgggcatc atcgaacagc gggcatctta
     1381 cccgtatctg tatcatttgt cgcagatccg ggagaatctg gtgagctttt tgccgtttga
     1441 taaggatatg cgggttctgg aatgtaatcc ggaatgcggg gcgctgaccg gaaagctgct
     1501 cgcaatggcg ggcggcgtga cggcgcttgc ggagagtgaa ctgcaggaga agattttgag
     1561 ggagcgcttt aaagaaccgc aggagaggga gaagcttgac ataagaagac agcttccgga
     1621 gcacgggcgg ttcgatgtga tcctgattgc cggccatttt taccgctggg aaaaacagct
     1681 tccggcgtta cgggagctgc ttgcgccggg aggaaagttt tacgtggcgg acgccaaccg
     1741 gatcgggctg aaatatcttg cgggatgtca ggaagaatac tgcggcggat attttaccgg
     1801 aattgacggt tatccggatg cggcggcggt tggacgcttt ggcagaagct acagccgggc
     1861 agagtacgca aggctgctgc aggaggcggg atttggggga ctgacgtttt attatcccta
     1921 tcccgatcac aagttcccgt ccgtgatcta ctcggacgag tggctgccgc agaaaggcga
     1981 actcgccgag gggcgcagca actatgaccg tgacagggtg gcatgcttca acgagcgtgt
     2041 gatgtttgat tccctgttgg aggagggcgt atttcagacg ttctccaatt cctttttgat
     2101 cgaagctgtg caggagggat agcgcgtttg gaacgaacga tnnnnnnnnn ntttattcaa
     2161 aatattccaa tgagcgggcg gaacggttcc gtatccggac ggatatagta accgatgaag
     2221 ccggagagaa aaaagtatat aagtacgcct gtaccataca ggcgggagac catatccgca
     2281 ggcaggaaga actcggaaag cagctggacg cggcatacgc gggcagccgc atcaccttct
     2341 gcccctgtac gacagaggat gtgccggggg gatgcagaag cgtgtcgccg tttgtgcagg
     2401 gggacaatct gcagcatctc atggaacagg cggtggcagc cggcgactgg gagactgtgg
     2461 aacagatggt cgcggcgtat gcagaccgtg tgttcggcag cggaggagag atcccctttg
     2521 acaggacgcc ggagtttgcg gaggtattcg gggagggaaa gcttccggag ggaataccct
     2581 gcgccgcagt cagcgatgtg gatatgatat tttccaatat ttttgtggag agcgggaaag
     2641 ccgccgcgga cagcgcgtgg atcgtcatcg attacgagtg gacattccca tttccggtgc
     2701 cgaagaaata tctgatctac cgtgc
//
LOCUS       DBMDQJ010000322         2710 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13335_length_2710_cov_1.409736, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000322 DBMDQJ010000000
VERSION     DBMDQJ010000322.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2710)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2710)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2710
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13335_length_2710_cov_1.409736"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            110..1195
                     /locus_tag="ACZO9R_07285"
     CDS             110..1195
                     /locus_tag="ACZO9R_07285"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate
                     methylesterase/protein-glutamine glutaminase"
                     /protein_id="HIR0912903.1"
                     /translation="MSKKILIVDDSALMRRVMCDIINSDGTFQVTDTCRDGLEAYEKL
                     KTTRYDGVVLDVNMPRMDGLQLLEQLQKDRIRATVIMVSTTTTKDADVTIRAMERGAV
                     DFVTKPTNVIEAKGEEFKKQLLDVLSAVCATQSYNRERRIAPQTKAVSAPAGTAKPVA
                     TAGHAIKSGSKIVALACSTGGPKALQSVIPYLPKNLDAPMVLVQHMPAGFTKSMADRL
                     DEVSPIHVKEAEDGDVLMKGTVYIAPGGKHMEVKRQPDGSHRIRLNDMPAIGGLKPCA
                     NITYDSLRDCGYDQVVCVVLTGMGADGTKGIVSLAGKKPVYVIAQDAASCVVYGMPKA
                     VAEAGVVDEVVPLNDVAKSIIKNVGVK"
     gene            1197..>2710
                     /locus_tag="ACZO9R_07290"
     CDS             1197..>2710
                     /locus_tag="ACZO9R_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR0912904.1"
                     /translation="MDVSQYLEIFIDETSEHLQNLSDCIMELEKDPENMDTINEIFRA
                     AHSLKGMAGTMGFKRMQRLTHDMENVFQEVRSEKVKVTSSMIDLLFKCLDAIEGYLDN
                     VKASSDEGTEDNELIIKELNDFIAAEDGAAAAPAQAEPKKEEAPAQTAGGDDKKYFEF
                     GLSESEKDKIKQAEEGGAHIYGMTIYIQKECLLKAARAFLVFKAVEEFGEILVYNPSS
                     QDIEDEKFDCDFSIFIASDEPYEKIAEAAKSVSEIDKVVGEEVKYETLAAKQEKAEAD
                     AAAAEKSQDAPAEAAPAQEQPQAQAAKPAAKTQSSGNKKQTVAKPVTNRTVRVDIEKL
                     DALMNQVSELIIAKNSLVSISSTDSGDFQNQSFHEQIEYLERITTNLHESVMKVRMVP
                     IESVVAKFPRMIRDLSRKLDKKMELYMTGEDTELDRTVVDQIGDPLQHLLRNSADHGL
                     EDNATRIAKGKPEVGSIFLNAFQEGNNVIIQVGDDGNGIDVEAVRNKAIERGVIT"
BASE COUNT          759 a          596 c          810 g          545 t
ORIGIN      
        1 cgaggccaga gccaaattcc tgattaccga cagcagcgta agggaggaaa tcatccggtt
       61 catctttgaa gaagaacgca aagcgcgtca aaggggtaga gcataagata tgagtaaaaa
      121 gattttgatt gttgatgact ctgcactcat gagaagggtc atgtgtgata taatcaattc
      181 agacggaaca tttcaggtca cagatacttg ccgtgacggt cttgaggcat atgagaagct
      241 taagacgacg cgctatgatg gcgttgttct cgatgtcaat atgccccgca tggacggact
      301 gcagcttttg gaacagttac agaaggacag aatccgtgca acggtcatca tggtaagtac
      361 gacgacaacc aaggatgcgg atgtcacgat ccgggcgatg gagcgcgggg cagtcgattt
      421 tgtcacgaag ccaaccaatg ttattgaggc aaagggcgag gagttcaaaa aacagcttct
      481 cgacgtgctc tcggcggtat gcgcgaccca gagttacaat cgcgaacggc gcatcgcacc
      541 gcagactaag gctgtatcgg ctcctgcggg gaccgcaaag ccggttgcaa cagccggaca
      601 tgcgatcaag agcggcagca agatcgttgc gcttgcatgt tcgacgggcg gtccgaaggc
      661 gctgcagagt gtcattccat atctgccgaa gaatctggac gcaccgatgg tactggttca
      721 gcatatgcct gccggattta ccaagtcaat ggcagacaga ctggacgagg tcagcccgat
      781 tcatgtcaag gaggcggaag acggagatgt gctgatgaaa ggaacggtgt acatcgcacc
      841 gggcggcaag cacatggaag tgaagcgcca gccggatgga agtcacagga tccggttgaa
      901 cgatatgcct gcgatcggcg gcttgaagcc gtgtgccaac atcacgtacg attccctgcg
      961 ggactgcggt tacgatcagg ttgtatgcgt ggttctcacc ggaatgggtg cggacggcac
     1021 caaggggatt gtttcccttg ccgggaagaa gccggtctat gtgatcgcac aggacgccgc
     1081 gtcgtgcgtg gtatacggaa tgccgaaggc ggttgccgag gcgggcgttg tggatgaagt
     1141 ggttcctctt aatgacgtag ctaaatcaat tattaagaat gtgggggtaa agtaatatgg
     1201 atgtaagcca ataccttgag atttttattg atgagacaag tgaacattta cagaatttaa
     1261 gtgactgcat catggagctg gaaaaagatc cggagaacat ggacacgatc aacgagattt
     1321 tccgtgcagc acactcctta aagggaatgg ctggaaccat gggctttaag agaatgcagc
     1381 gcctgacaca tgacatggag aatgtgttcc aggaggtccg cagtgagaaa gtcaaagtga
     1441 ccagttctat gattgacctt ctgttcaagt gcctggatgc gatcgagggc tatctcgaca
     1501 acgtaaaggc aagttcggat gagggaacag aggacaacga actgatcatc aaggaactga
     1561 atgatttcat cgcggcggag gatggcgcag cagctgcacc tgcacaggcg gagccgaaaa
     1621 aagaagaggc accggcacag accgcgggcg gagatgataa aaagtatttt gagttcggtc
     1681 tcagcgagtc cgagaaggat aaaatcaagc aggcggaaga gggcggcgca cacatttatg
     1741 gcatgactat ctatatccag aaagagtgcc tgttaaaggc agcgagagca ttcctggtat
     1801 tcaaggcagt ggaagagttt ggagagatcc ttgtatacaa tccgagctcc caggatattg
     1861 aggacgagaa gttcgactgc gatttcagca tttttattgc gtcggacgag ccgtatgaga
     1921 agattgcgga agctgcaaag tcggtatccg agattgataa ggttgtcggt gaggaagtaa
     1981 aatacgagac gctggctgca aagcaggaga aagcggaagc ggacgctgcg gcggcagaga
     2041 agtcccagga tgcaccggct gaggcggcac cggcacagga gcagccacag gcacaggcag
     2101 caaagccggc agcgaagaca cagagcagcg gcaataaaaa gcagacggtt gcaaagcctg
     2161 tcacaaaccg tactgtccgt gtagatattg agaagctgga tgcattgatg aaccaggtat
     2221 ccgagcttat catcgcaaag aacagtctgg tgtcgatcag ctccaccgat tccggagatt
     2281 tccagaacca gtccttccat gagcagattg aatatctgga gcgtatcacg accaatcttc
     2341 atgagtccgt gatgaaagtc cggatggtgc caatcgagag tgtagttgca aaattcccgc
     2401 gtatgatccg tgatctttcc agaaagctgg acaagaagat ggaattgtac atgacaggtg
     2461 aagatacaga acttgaccgt accgtagtcg atcagatcgg tgacccgctg cagcatctgc
     2521 ttcgtaattc cgcggatcac ggacttgaag acaacgcaac ccgtattgca aagggcaagc
     2581 cggaagtcgg aagcattttc ctcaacgcat tccaggaagg caacaatgtc atcatccagg
     2641 ttggcgatga cggtaacgga atcgacgtgg aggctgtgcg caacaaggca atcgagcgcg
     2701 gtgtcatcac
//
LOCUS       DBMDQJ010000323         2682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13491_length_2682_cov_1.515692, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000323 DBMDQJ010000000
VERSION     DBMDQJ010000323.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13491_length_2682_cov_1.515692"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="ACZO9R_07295"
     CDS             <1..391
                     /locus_tag="ACZO9R_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HIR0912905.1"
                     /translation="GVLVNPDRSVRQAGGFIIQLMPFTPDDVVDRLEKKITEIASVTE
                     MLEDGKTPEEILELILGEFGLEINDTVDAAFQCDCSKERVSRAIATLSRKDLDDIVSD
                     GESIEVKCQFCNKAYHFDIDELKEMRK"
     gene            388..2298
                     /locus_tag="ACZO9R_07300"
     CDS             388..2298
                     /locus_tag="ACZO9R_07300"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HIR0912906.1"
                     /translation="MRDGFVKVAAVTPKIRVADTTYNAGVIVEGIREAAAAGAKVIVL
                     PELVITGYTCSDLFLQEYLLEQAKRALGTIAEETAEVDAILFVGLPLAYNGKLYNVAA
                     VLCRGEVLGFVPKTYLPNYNEFYEARHFARGMADPVEVDFEGECVPMGTRLLFVCTSL
                     PELKIGAELCEDLWTPEPPSIRHARNGATLLVNLSASDETTGKDIYRRELVNGQSARL
                     LCGYIYASAGDGESTQDVVYSGHNVIAENGHILKESERFGSGVICTEIDVKRIEAERR
                     RMTTFEMAEDGYVEVRFSLETEETALTRFIDPMPFVPGNEADRIRRCDEILAIQAAGL
                     KKRLEHTHCKTAVVGISGGLDSTLALLVTVRAFDLLGIPHDRIKAVTMPGFGTTDRTY
                     DNAVSLIRCLGADFTEVDIKEAVNVHFRDIGQDPAVHDVTYENGQARERTQILMDIAN
                     KEGGMVIGTGDLSELALGWATYNGDHMSMYAVNASVPKTLVRHLVRYYADTCGDEKLG
                     AVLTDVLDTPVSPELLPPEDGKISQKTEDLVGPYELHDFYLYHMLRLGFAPKKVYRLA
                     KVAFDGVYDDETILKWLKTFYRRFFAQQFKRSCLPDGPKVGSVAVSPRGDLRMPSDAC
                     AAVWLQQLEEIG"
     gene            2301..>2682
                     /locus_tag="ACZO9R_07305"
     CDS             2301..>2682
                     /locus_tag="ACZO9R_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR0912907.1"
                     /translation="MQYRKDKNGNEISVLGYGCMRFTKEGGHADIDKADKEVMAAIDA
                     GVNYLDTAYVYAGNEAAVGEILARNHCRERIYLATKLPHYLIKSVEGAQKMFEEELRR
                     LQTDYLYCRTEVLYVIHAGEGAGHN"
BASE COUNT          606 a          628 c          878 g          570 t
ORIGIN      
        1 cggtgttctc gtgaatccgg accggtcggt cagacaggcg ggaggattta tcatccagct
       61 gatgccgttt acaccggacg acgtggtgga tcgtctcgag aagaagatca cggagattgc
      121 ctccgtcacg gagatgctgg aagatgggaa gacaccggag gagatcttgg agctgattct
      181 cggcgagttc gggcttgaga tcaacgatac ggtggatgcc gcattccagt gcgactgctc
      241 caaggagcgc gtctcccgtg cgattgcgac actgagcaga aaggatctgg acgacatcgt
      301 aagcgacgga gagtcgattg aggtaaaatg ccagttctgt aacaaagcgt accattttga
      361 catcgacgag ttaaaggaaa tgagaaaatg agagatggat ttgtaaaagt tgccgccgtc
      421 acaccgaaga tccgtgtggc ggacacaaca tacaacgccg gcgtgatcgt ggaaggaatc
      481 cgggaagcgg cggcggcagg cgcgaaggtg atcgtgctgc cggagcttgt catcacggga
      541 tatacctgca gtgatctgtt tttgcaggaa tatcttttag agcaggcgaa gcgggcgctc
      601 ggaacgatcg cggaggagac agcagaggtg gacgccatcc tttttgtcgg tctaccgctt
      661 gcgtacaacg gaaaacttta taatgtggca gcggtgctct gccgcgggga agtgctcggg
      721 tttgtgccca aaacctatct tccgaactac aatgaatttt acgaggcaag acattttgcc
      781 agaggaatgg cagatccggt cgaggtcgat ttcgagggag agtgcgtgcc gatggggacg
      841 aggcttttat ttgtgtgtac gtctctgcca gaattaaaga tcggcgcgga gctttgcgag
      901 gatttgtgga cgccggagcc gccgagtatc cgtcatgcca gaaatggtgc gacgctgctt
      961 gtgaatctgt ctgcatcaga tgagaccact ggaaaggaca tctaccggcg ggagctggta
     1021 aacggacagt cggcgagact tctgtgcggc tatatctatg cgagtgcggg agacggggag
     1081 tccacgcagg acgtcgtcta ctccggacac aatgtgattg ccgagaacgg tcatattcta
     1141 aaggaatcgg agcggttcgg aagcggtgtg atctgcacgg agatcgatgt caagcggatc
     1201 gaggcggagc gccgccggat gacgacgttt gagatggcgg aggacggcta tgtggaggtg
     1261 cgtttttctc ttgaaacgga ggagacggct ctgacccgct tcatcgaccc gatgccgttt
     1321 gtgccgggaa atgaggcgga ccgtatcaga cgctgtgatg agatccttgc cattcaggca
     1381 gcgggattaa aaaagcgtct ggagcatacg cactgtaaga cggcggttgt gggcatttcg
     1441 ggtggtctgg attccacgct ggcactgctt gtcaccgtgc gtgcgttcga tctgctcggc
     1501 atcccgcacg accggatcaa ggcagtcacg atgccgggct tcgggacgac ggatcggacc
     1561 tatgacaacg cggtgtccct gatccggtgc ctcggcgcgg attttacgga agtcgacatc
     1621 aaggaagcgg tgaatgtgca tttccgtgat atcgggcagg atccggccgt tcatgatgtg
     1681 acctatgaga acggccaggc aagagagcga acgcagattc tgatggacat tgccaacaag
     1741 gagggcggca tggtcatcgg aaccggcgat ctctcggagc ttgcactcgg ctgggcgaca
     1801 tataacggcg accatatgtc catgtatgcg gtcaatgcct ccgttccgaa gacgctggtg
     1861 cgccacctgg tgcgctacta cgcggatacc tgcggggatg agaagcttgg cgcggtattg
     1921 acggatgtgc tggacacgcc ggtatctccg gagcttctgc cgccggagga cggaaagatc
     1981 tcgcagaaga cggaagatct tgtgggaccg tacgagcttc atgattttta cctgtaccac
     2041 atgctgcgtc ttggatttgc accgaaaaag gtgtaccgtc tcgcaaaagt ggcgtttgac
     2101 ggcgtctacg acgatgagac gatcttaaaa tggctgaaaa ccttttaccg gcgctttttt
     2161 gcacagcaat tcaagcgttc ctgtctgccg gacggaccga aagtcggtag tgtggcggtc
     2221 tctccgcgcg gggatctgcg gatgccgtcg gacgcgtgtg cggctgtctg gctgcagcag
     2281 ttggaggaga tcggatgatc atgcagtatc gcaaggataa aaacggaaat gagatttcgg
     2341 tgctcggcta cgggtgtatg cgttttacca aggagggcgg gcatgccgat atcgataagg
     2401 cggacaagga ggtcatggcg gcgatagatg ccggtgtgaa ctatctcgat accgcatatg
     2461 tctatgccgg aaatgaggcg gcggtcgggg agatcctcgc gcgcaatcat tgcagggaac
     2521 gcatctacct ggcaaccaag ctgccgcact acctgatcaa gtcggtggag ggcgcccaga
     2581 agatgttcga ggaggagctg cgccgcttac agacagatta tctgtattgt cggaccgagg
     2641 tgctttacgt aattcacgcg ggcgaaggtg cagggcataa ct
//
LOCUS       DBMDQJ010000324         2678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13520_length_2678_cov_1.759798, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000324 DBMDQJ010000000
VERSION     DBMDQJ010000324.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13520_length_2678_cov_1.759798"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="ACZO9R_07310"
     CDS             <1..696
                     /locus_tag="ACZO9R_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912908.1"
                     /translation="AEWKRLSRGCRMYTAYAACMLVLVGNPFGYNDISTFWMETSYWK
                     MFFLLLPAVAAAIPVTELAVGTKHVWTGALGVLVCAALVAVSMSFQFTAPKLAVPENA
                     YHVEHEIAELDDALRESGRNIQNMIAPRAVCAQIRELDASVNLLYGEDLIERMIAQDT
                     VTEDETEKQFISDCASIVAVPAAVDNQIAMAEKYNSNCVILEADYDDQTLMESAGYDC
                     YGTAGSYVIYFRE"
     gene            717..2066
                     /locus_tag="ACZO9R_07315"
     CDS             717..2066
                     /locus_tag="ACZO9R_07315"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR0912909.1"
                     /translation="MRLREQYKHLLNYGANLITLLVEGFCFGYIWYTVYEEQFGFWRR
                     GNWAVVGLYVLVIFFFTKVFGGYNIGYMRMTDIALSHILSILLSGIVGYLELCLICRD
                     YVEPAPMLGVMAVEIVFILPWIYIIRKLYAKLYPPRQMLVIYGHYAPDELIAKINTRR
                     DKYNICGSVSYEIGHEKLYPMIREYNAVVLCDLPAQARNQIMKFCYQESIRTYVTPKI
                     SDIIFRGADDIHLFDTPLLLSRNQGLSIDQRIIKRTCDIVLSLIGIVIASPFMLIIAL
                     AVKLYDGGPVFYKQERLTRDEKPFMIYKFRSMSMDSEKNGARLAAKGDKRVTPVGKII
                     RNIHFDELPQLFNILKGDMSMVGPRPERQVIADQYAEEIPEFVFRTKVKAGLTGYAQV
                     YGKYNTTPYDKLKFDITYIENYSLWLDVKIMLLTFKILFQKENTEGVDETQQTALKD"
     gene            2076..>2678
                     /locus_tag="ACZO9R_07320"
     CDS             2076..>2678
                     /locus_tag="ACZO9R_07320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912910.1"
                     /translation="MIQVTAITAAYNAASYIENCIQSVLAQTVPCRMVIVDDASLDDT
                     YAIAMRYAEEYPDRITVLKNEQNAGAAASRNRAVACATTPYVAILDADDWWQEDKTER
                     QLKRLAETKADACYAGRELMHADGISTTKIVQVPETVTYHGLLKGNVIPCSSVLMRRE
                     DALQYPMTHDELHEDYIVWLSMLRDGKTFAGVNEPLLKSRL"
BASE COUNT          644 a          626 c          798 g          610 t
ORIGIN      
        1 gcagagtgga agcggcttag ccgcgggtgc cgcatgtata cggcctacgc cgcgtgtatg
       61 ctggtacttg tggggaatcc gtttggatac aatgacatca gcaccttctg gatggagacg
      121 tcttactgga aaatgttttt cctgctgctt ccggcggtcg cggctgcaat cccggtgacg
      181 gagcttgcgg tcgggacaaa gcatgtgtgg acgggagcgc tcggcgtgct ggtgtgtgcg
      241 gcgctcgtgg cggttagtat gagttttcag tttacggcgc caaagcttgc cgtgccggag
      301 aatgcatacc atgtggagca tgagattgca gaactcgacg atgcgctgcg ggagtctggc
      361 agaaacatac agaatatgat cgcgccgcgt gcggtctgcg cacagatccg cgagctggac
      421 gcctcggtga atctgctcta cggagaggat ctgatcgagc ggatgatcgc acaggatact
      481 gtgaccgaag atgagacaga gaagcagttc atcagtgact gtgcaagcat tgtggcggtg
      541 ccggcggcag tcgacaacca gattgccatg gcggaaaagt ataacagcaa ctgcgtgatc
      601 ctggaagcgg attatgacga tcagacgctg atggagagcg ccgggtacga ctgctatggc
      661 acagccggtt catatgtgat atactttagg gaataatagc aaaggaatcg aactgaatga
      721 gattaagaga gcaatataag catctattaa attacggagc gaatctgatc actctgctgg
      781 tggagggatt ctgctttggc tacatctggt acactgttta cgaggagcag tttggtttct
      841 ggcgccgtgg aaactgggcg gtcgtcggac tgtatgtgct ggtgatcttc ttttttacga
      901 aggtatttgg cgggtataat atcggctata tgcggatgac ggatattgca ttgtcccata
      961 ttttatccat tttactgagc gggatcgtcg gttatctgga gctctgcctg atctgccgtg
     1021 attatgtgga gcccgcacct atgctcggcg ttatggcagt tgagatagtc ttcatcctgc
     1081 cgtggatcta catcataaga aaactctatg ccaagctgta cccgccgcgg cagatgcttg
     1141 tcatctacgg acattatgcg ccggacgagt tgatcgcgaa gatcaatacg cgccgtgaca
     1201 agtacaatat ctgcggatcg gtcagctatg agatcggtca tgagaagctt tacccgatga
     1261 tccgcgaata caatgcggtg gtgctctgcg atctgccggc acaggcgagg aaccagatca
     1321 tgaagttctg ttaccaggag tcgatccgga cctatgtcac accgaagatc tcagacatta
     1381 ttttccgcgg agcggacgac atccatctgt tcgacacgcc gctgctgtta tcgcgcaatc
     1441 agggactgtc gatcgatcag cgcatcatta agagaacctg cgatattgtg ctgtcactga
     1501 tcggtattgt gatcgcgtcg ccgttcatgt taatcattgc actggcggtc aagctgtacg
     1561 acggaggtcc ggtgttctac aagcaggagc gcctgaccag ggatgagaag ccttttatga
     1621 tctacaagtt ccgcagcatg agcatggatt ccgagaaaaa cggcgccaga cttgcagcaa
     1681 agggcgacaa gcgcgtgacg ccggtcggaa agatcatccg caatattcat tttgatgagc
     1741 tgccgcagct gtttaatatc ttaaagggcg atatgtccat ggtaggaccg agaccggagc
     1801 gccaggtcat cgcggaccag tatgccgagg agattccgga gttcgtgttc cgcaccaagg
     1861 tgaaggccgg actgaccgga tacgcgcagg tgtatggaaa atacaatacc acgccgtacg
     1921 acaagctgaa gttcgacatc acatacattg agaactattc cctgtggctc gacgtcaaga
     1981 ttatgctgct gacgttcaag attctcttcc agaaggagaa taccgaggga gtggatgaaa
     2041 cgcagcagac ggcactgaaa gattaggaga tttcaatgat acaggtgacg gcaatcaccg
     2101 cagcttataa tgctgcatcc tatattgaaa attgtataca gtccgtgctg gcacagaccg
     2161 tgccgtgccg gatggttatt gtggacgatg catccttgga tgacacgtat gccattgcca
     2221 tgcgctatgc agaagagtat cccgaccgga tcacggtgct aaaaaacgag cagaatgccg
     2281 gggcggcggc atcgcgcaac cgtgcggttg cctgtgccac gacgccctat gtggcgatat
     2341 tggatgcgga tgactggtgg caggaggaca agacggagcg tcagctaaag cggcttgcgg
     2401 agacaaaggc agacgcctgc tatgcgggca gggaactgat gcacgccgac gggatctcta
     2461 ccacgaagat cgtgcaggtt ccggagacgg tcacgtatca cggacttctc aaggggaacg
     2521 tcatcccgtg ttcttccgtg ctgatgcgca gggaggacgc cctgcagtat ccgatgacac
     2581 acgatgagct gcacgaagac tacattgtgt ggctgtccat gttaagggac gggaaaacct
     2641 ttgccggtgt aaacgagcct ttattaaaat ccaggctg
//
LOCUS       DBMDQJ010000325         2672 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13553_length_2672_cov_1.269156, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000325 DBMDQJ010000000
VERSION     DBMDQJ010000325.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13553_length_2672_cov_1.269156"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1905)
                     /gene="nrdD"
                     /locus_tag="ACZO9R_07325"
     CDS             complement(<1..1905)
                     /gene="nrdD"
                     /locus_tag="ACZO9R_07325"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HIR0912911.1"
                     /translation="MKIIKRSGSEDIFDISKIEAAIRKANDSVIDYEKLGDDKIQAIL
                     SNVEKACENMKRSPSVEEIQDMVENQIMSQKAFNVARNYITYRYKRELVRKSNSTDEQ
                     ILSLLECNNEEVKQENSNKNPTVNSVQRDYMAGEVSKDITRRFLLPPEIVEAHEKGII
                     HFHDADYFAQHMHNCCLVNLEDMLQNGTVISETMIDTPKSFSTACNIATQAIAQIASS
                     QYGGQSISLSHLAPFVQVSREKFRKQVRKELETAGIEATEEKVNQMAEMRVKEEINRG
                     VQMIQYQVITLMTTNGQAPFITVFMYLDEVPEGQLRDDLAAVIEEMLHQRILGVKNEQ
                     GVYITPAFPKLIYVLEEDNIREGSKYWYLTRLAAECTAKRMVPDYISEKMMKKLKDGD
                     CYTCMGCRSFLTVDRTTGNLAHAKNYKEGKHKYYGRFNQGVVTLNLVDAACSSGGDEK
                     KFWEIMDERLEMCYQALMIRHNRLKGTPSDVAPILWQNGALARLKKGETIDELLYHGY
                     STISLGYAGLCECTRYMKGVSHTEPEGTEFALAVMRRLNDACAKWKKETDIDFSLYGT
                     PLESTTYKFAKCLQKRFGIIPGVTDKNYITNSYHVRVTEQINAFDKLKFESQFQELSP
                     GGAISYVEVPN"
     gene            complement(2037..2522)
                     /locus_tag="ACZO9R_07330"
     CDS             complement(2037..2522)
                     /locus_tag="ACZO9R_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0912912.1"
                     /translation="MERIKRSETIGYKIRLLHNQIHKNMEAKRMENEDNLTGMQRWTL
                     GFLKDHSDCEVYQKDIEEAFSISRATASNMLGVMERKGLIKRVAVSHDARLKRLVLTD
                     KAAALLDGAERDIREMEARLTAGLSAEDVEHLKRCLDQMLQNLDVTIAEDTRCCCKPK
                     R"
BASE COUNT          598 a          733 c          551 g          790 t
ORIGIN      
        1 attcggaacc tccacgtagc tgattgctcc gcccggggac agttcctgga actgtgactc
       61 gaactttaac ttatcgaacg cgttgatctg ctcggtcaca cgtacatgat agctgtttgt
      121 gatgtagttc ttgtccgtca cacccgggat aatgccgaaa cgcttctgta agcattttgc
      181 gaacttgtag gtcgtcgact caagcggggt tccgtagaga ctgaaatcaa tgtccgtctc
      241 cttcttccac ttggcgcatg catcattcag acgacgcatc actgcaagcg cgaattccgt
      301 gccttccggc tccgtgtggg atacgccctt catatagcgt gtgcactcgc agagtcccgc
      361 atagccgagg gaaatcgtcg agtaaccgtg atagagaagt tcatcgatcg tctcgccttt
      421 cttcagacgt gcaagcgcac cgttctgcca gagaatcggc gccacgtcgg aaggtgttcc
      481 ctttaagcgg ttgtggcgga tcatcaatgc ctgatagcac atttccagac gctcatccat
      541 gatctcccag aacttcttct catctcctcc ggaagagcat gccgcatcca ccaggttcaa
      601 tgtcactacg ccctggttaa agcgtccgta atacttgtgc tttccttctt tataattctt
      661 ggcatgtgcc agattgcccg tcgtgcggtc taccgtcagg aaagaacggc atcccataca
      721 ggtataacag tcgccgtctt ttaacttttt catcatcttc tcagagatat aatccggcac
      781 catgcgcttt gcggtacact cagccgcaag cctggtcagg taccagtatt tggacccctc
      841 cctgatattg tcctcctcaa gcacatagat cagtttcggg aacgccggcg taatataaac
      901 gccctgctca ttcttgaccc ctaaaattct ctgatgaagc atctcctcga tcactgctgc
      961 cagatcgtcc cgaagctgtc cctccggcac ctcatccaga tacataaaca ccgtgataaa
     1021 cggagcctgt ccattggtcg tcatcagcgt gatgacctgg tactggatca tctgcacacc
     1081 gcggttaatc tcctctttga cccgcatttc cgccatctgg ttgactttct cttccgtcgc
     1141 ctcgattccg gcagtctcaa gctccttgcg cacctgcttg cggaacttct cacggctcac
     1201 ctgcacaaac ggagccagat gagacaggga aatactctgt ccgccgtact gggaacttgc
     1261 aatctgcgcg attgcctgtg tggcaatatt gcaggcagtg gaaaagctct tcggcgtatc
     1321 gatcatcgtc tcgctgatga ccgtaccgtt ctgaagcatg tcctccagat tgaccagaca
     1381 gcagttgtgc atgtgctgcg caaaataatc cgcgtcatga aaatggatga ttcctttctc
     1441 atgcgcctct acgatctccg gtggaagcag gaatcttctg gtgatatcct tgctgacctc
     1501 accggccata taatctcttt gtacgctgtt tacagtcgga ttcttgttgg agttctcctg
     1561 ttttacttcc tcgttattgc attccagcag acttaaaatc tgctcatcgg tagagttgga
     1621 cttgcgcacc agttctctct tataacgata agtaatatag ttgcgggcaa cattaaacgc
     1681 tttctgactc atgatctggt tctccaccat atcctgaatc tcctccacac tcggggagcg
     1741 cttcatattc tcgcaggctt tttcaacatt ggaaagaatc gcctgaatct tgtcgtctcc
     1801 aagcttttca taatcaatga cgctgtcgtt tgccttgcgg attgcagcct cgatcttgct
     1861 gatgtcaaaa atgtcctcgc tgccacttct cttaataatc ttcataaccg ttcagaatcc
     1921 tttctgttaa ttgctttttc attatacaat atctagtatt gttgtcaatg cgttttccta
     1981 gattctgtgg ttattttacc ttttgaacaa aattcatccc gaaactttgt cactttttat
     2041 cgctttggtt tacaacaaca tcttgtgtct tctgctattg tcacatccag attttgtagc
     2101 atctgatcca ggcagcgctt caggtgctcc acatcctccg ccgaaagccc cgccgtcaga
     2161 cgcgcttcca tctcccgtat atcgcgctcc gcgccgtcta aaagcgcagc ggccttatct
     2221 gtgagcacca gacgttttaa gcgcgcgtcg tgtgagactg ccacacgctt tatcagcccc
     2281 ttgcgctcca tcacaccaag catattagaa gcggtcgctc ttgaaatgga aaaagcctcc
     2341 tcgatatcct tctggtagac ttcacagtcg ctgtgatctt tcaaaaaacc aagtgtccag
     2401 cgctgcattc ctgtcaggtt atcctcattt tccatacgct ttgcttccat attcttatgg
     2461 atctgattgt ggagcaggcg tattttgtat ccgatggtct ctgacctttt gattcgttcc
     2521 atctcttcct ctcttctgtc tgttttctaa aataatcatt gacttctgtt tttaattatc
     2581 gtataacatt attgttagca cactaacaat taaaaatcaa cagaaaattc aaaaaggatg
     2641 tgaaattatg ttaaaaacac ttggagcaca ga
//
LOCUS       DBMDQJ010000326         2670 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13567_length_2670_cov_1.879330, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000326 DBMDQJ010000000
VERSION     DBMDQJ010000326.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13567_length_2670_cov_1.879330"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(109..>2670)
                     /locus_tag="ACZO9R_07335"
     CDS             complement(109..>2670)
                     /locus_tag="ACZO9R_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR0912913.1"
                     /translation="GNCEEARDYYQEFVEIAPHDNLKYVLRYEISKAEGADLQTLIGI
                     LEELKEQEYSEEWAFELACLYHKAGMSEKCIDACDELILWFGEGPYVERALELKMLYQ
                     PLTRQQEEKYRQFRLKKEGVVEVRPEDDLESGEIVSEPVQIPKVKLSPERFNTQNLQD
                     ELRKNMVQIMEATDKEKVEDSMDTIKKLVEEIPYLQIPKEEPLPQDTIEMPHIETDAE
                     IDNSLKSNFKELLAEDYDGQISMYVPDKGATEPQVTGQMSIDEVLADWEKTKRAAETA
                     LEEAKQRKLESAKARALQEAGDIMERLVDVIPKLDSGLTPKDLLDEEYLGGQPIADDK
                     AAEMVANMNQILQQEIDRLSSENARMDEQLAAASAAEAAGVAEPQEALQPEEADFWGN
                     GITELTQSPYEIEPDAEQLAAEAAYEPQTDAVYGAETEPVYAPEAEAVYEPQTGAVYE
                     PQTDAVVSETEQADAAGSYGGIEEPEIPGSYTEETDPDTFTLGVDVQALLAEEAVAKD
                     TAQPQAAEEPEDTDDAAGMEEPDTAALGADIQALMEEQKIQPEDEELPKAEKSGGKLA
                     GGGHPKSGASKAKAADDVLPKIEEPEEEGHQAIIRLSKEQREIFTYFVPIKGMEQQLC
                     QALTGAAARLSSGKTASTGNMIIQGGQGSGKTVLATSMIKALQKEVGKPNGKIGKIEA
                     SVLNQKDVAALLKKVAGGCLIIEKAGAISRETAVKLGTLLDSDNSGLFVIMEDTRRGI
                     EKALSRDAGFAARFSEKINIPIFTSDELVAFARSYANELGYTIDEMGVLALYNSISNI
                     QKLDQATTLTEVKDIVDEAIAHAERGGLKKAFSIITSRRYDDDDYVILHEKDFGF"
BASE COUNT          461 a          882 c          568 g          759 t
ORIGIN      
        1 atgtcttcca gtactttgta aatagcattc actccttcat tgcgtgcggc atgtccggct
       61 tttccgtatg cggtaacatc cagcaccatc taccccttta tcttgatttt aaaatccaaa
      121 atctttttca tgaaggatga cataatcgtc atcgtcatat cttctggaag tgataatact
      181 gaacgccttc ttaagtcctc cccgttctgc atgggcgatt gcctcgtcca caatatcctt
      241 gacctctgta agggttgtcg cctgatccag cttttgaata ttgctgatgc tgttgtaaag
      301 tgccagaact cccatctcat cgatggtgta acccagttcg ttggcatagg atctcgcaaa
      361 cgccaccagt tcgtcgctcg taaagatcgg gatattgatt ttctccgaaa accgtgctgc
      421 aaatcccgca tcccggctga gtgccttctc gattcctctt ctggtgtcct ccataatcac
      481 aaacagtccg ctgttgtcac tgtcaagcag cgtgccaagc ttgactgccg tctctcttga
      541 gatggctccc gccttttcga taatcagaca tccgcccgcc acttttttaa ggagcgccgc
      601 cacatctttc tgattcagca cggaagcttc gatcttgccg atttttccgt tcggcttgcc
      661 cacttccttc tggagcgcct taatcatgct ggtcgcgagc accgtcttgc cgcttccctg
      721 tccgccctga ataatcatat tcccggtgga agccgtttta ccggacgaaa gtcttgccgc
      781 tgctcccgta agcgcctggc acagctgctg ttccattccc ttaatcggca caaaatatgt
      841 aaaaatctcc cgctgctctt tggaaagccg gataattgcc tgatgtcctt cctcctccgg
      901 ctcctcgatc ttcggaagca cgtcgtctgc cgctttcgct tttgaagctc cgctcttcgg
      961 gtgtcccccg cctgccagct ttccgccgga cttctccgcc ttcggaagtt cctcgtcctc
     1021 cggctgtatc ttctgctctt ccatcaatgc ctggatgtcg gctcccagtg ctgccgtatc
     1081 cggctcttcc atcccggcag catcgtctgt atcctccggt tcttccgccg cctgtggctg
     1141 cgccgtgtct tttgccaccg cttcctctgc cagaagcgcc tggacatcga cccccagggt
     1201 aaacgtatcc gggtctgtct cttcggtata gctgcccgga atctccggtt cctcgattcc
     1261 gccatagctt cctgccgcat ctgcctgttc cgtctcactt accaccgcat ctgtctgcgg
     1321 ttcatatacc gcccctgtct gcggctcata taccgcctct gcctccggtg cgtataccgg
     1381 ctctgtctct gctccgtata ccgcatctgt ctgcggttca tatgcagcct ccgccgcaag
     1441 ctgctccgca tctggttcta tctcatacgg gctctgtgtc agctctgtga tgccgttccc
     1501 ccagaaatct gcctcctccg gctgcaacgc ttcctgcggc tccgccacac ctgctgcctc
     1561 cgccgcactt gccgccgcaa gctgttcatc cattctcgca ttctcggagg ataagcggtc
     1621 gatctcctgc tgtaagatct ggttcatgtt cgccaccatc tcagccgcct tatcgtcagc
     1681 aatcggctgg ccgcccagat actcttcatc cagcagatcc ttcggtgtca gtccggagtc
     1741 aagcttcgga atcacatcca ccagacgctc catgatatcg cctgcctcct gcagtgcacg
     1801 cgccttcgcg gattccagct tgcgctgctt cgcctcctca agcgccgtct ctgccgcacg
     1861 cttggtcttc tcccagtctg caagcacctc atcaatactc atctgtcccg tcacctgcgg
     1921 ctctgtagcc cccttatccg gaacatacat gctgatctgt ccgtcataat cctctgcaag
     1981 aagctcttta aaattgctct tgagcgagtt gtcaatctct gcatccgtct cgatatgggg
     2041 catctcgatc gtatcctgcg gcagcggttc ctccttggga atctgaagat acggaatctc
     2101 ctccacgagc ttcttgatcg tatccatgct gtcctcgacc ttttccttgt cggtcgcttc
     2161 catgatctgc accatattct tgcgaagttc atcctgcaga ttctgtgtat taaaacgctc
     2221 cggtgaaagc tttaccttcg gtatctgcac cggttcgctc acaatctcgc cggactcaag
     2281 gtcgtcctcc ggccgcactt ccacaacgcc ctcttttttg aggcggaact gccggtactt
     2341 ttcttcctgc tgtctggtca gcggctgata gagcatctta agctccagcg cccgctccac
     2401 ataaggtcct tctccgaacc agagaatcaa ctcatcgcag gcgtcgatgc atttttcact
     2461 catccccgcc ttatggtaaa ggcatgccag ttcaaacgcc cactcctcgg aatattcctg
     2521 ctcttttaat tcttccagta ttccgatcag cgtctgcaga tcggcgccct ccgccttact
     2581 gatctcgtag cgcagaacgt atttgagatt gtcgtgcggg gcgatctcca cgaattcctg
     2641 atagtagtct ctcgcttcct cgcagttccc
//
LOCUS       DBMDQJ010000327         2664 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13617_length_2664_cov_1.224737, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000327 DBMDQJ010000000
VERSION     DBMDQJ010000327.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13617_length_2664_cov_1.224737"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(57..878)
                     /locus_tag="ACZO9R_07340"
     CDS             complement(57..878)
                     /locus_tag="ACZO9R_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-fucosyltransferase family protein"
                     /protein_id="HIR0912914.1"
                     /translation="MIIIEPCAGLGNKLLGMSSAYAVAQKLNRELIVMWKREVGCNVK
                     AEELFDLPFRVIEISENGYSKEPVAHFRGNQLKKKWRAKADRFLECGDVEAIKKQKGY
                     EGLLAVIEKEPVIYIKSFGPLCELDAASLAFLKPSAGILQKGEPLFSQLGAKTVGVHV
                     RRTDHTEAIANSPLSLFADKMRQEMTEVPGTDFFVATDDEGVKRELREALPDAKLIFH
                     DSGIIDRNSKEGICDAYIEMLALSRCRKILGSYNSTFSLLPSYIGDIPLEVVKKD"
     gene            complement(898..2634)
                     /locus_tag="ACZO9R_07345"
     CDS             complement(898..2634)
                     /locus_tag="ACZO9R_07345"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0912915.1"
                     /translation="MNLVKSVFKVFDGKQKRKLFGMAFLILINSGVSLLGVSVLLPFI
                     QAVTAPEELLANRYVAAFYEWMGMENPNELITAIAIGIVIVYLAKNAFIIFMNNMQYR
                     FSYYGKREMQDRLMKYYISKDYTFFLNHNSAELMRDINNDPEMFYAAVLNMLQLAAEL
                     CTSTILIIFLVVTDPIITLGVALAMMLMVLLFMKKLKRTLARFGDERRKYNANIIQCM
                     QQAFGGIKEIKIANRESYFEEEFRKQNGLYTYVIKQNAFLSSIPKPVMEALCIAGLMI
                     TIAVRINMTESDPTHFVGVLAVFAAAAFYLLPSANKMSEYLASIIHNGVVIHKIGEEY
                     TKIRDLEIDLSEERDYPAMALERAIRVNDMTFTYPEMEEPVLSHVNVTIPKNSSVAFI
                     GPSGAGKTTMVDLILGVLKPQEGQITLDGVDISTSYRGWHDKIGYIPQTIYMLDDTIR
                     NNIAFGKKEGINDEDVWAALRQAQLDEFVESLEEGLDTVIGEAGVRLSGGQRQRIGIA
                     RALYRKPEVLVLDEATSALDTETEAAVMQAIDSLQGKMTMLIIAHRLSTIKNCNIVYK
                     VENGSVTVQDAQ"
BASE COUNT          627 a          789 c          565 g          683 t
ORIGIN      
        1 tggtcaccag aatccgggtg cccagtatgt cacagcattt catctctatc ataaatttaa
       61 tccttcttta caacctcaag cggtatatcg ccgatatagg acggaagcag gctgaatgtg
      121 ctgttatagc ttccgagaat cttccggcat ctggagagcg ccagcatctc gatgtaagcg
      181 tcgcagattc cctctttgga attgcggtca atgataccgg aatcatggaa gatgagcttt
      241 gcgtccggca gtgcttcccg cagttcgcgc ttcacccctt catcgtccgt cgccacaaaa
      301 aagtccgtcc ccggcacttc cgtcatctcc tgccgcatct tatccgcaaa cagcgacagc
      361 ggactgttgg cgatcgcctc ggtgtgatcg gtacgtctga catgcacccc caccgtcttt
      421 gcgccaagct gggaaaacag cggttctccc ttttgcagaa tacccgcgct cggctttaaa
      481 aaggcaaggc ttgccgcatc cagctcacag agcggtccga aggacttgat atagatgacc
      541 ggctccttct cgatgactgc cagcagtccc tcgtagcctt tctgcttttt gatcgcttcc
      601 acatccccgc actccaaaaa acggtctgcc ttcgcgcgcc atttcttttt taactgattc
      661 cctctgaaat gcgcaaccgg ctccttggag tatccgttct cgctgatctc gatcacacgg
      721 aacgggagat caaacagttc ttctgctttt acgttacagc caacctcacg cttccacatg
      781 acgatcagct cacggtttaa cttctgtgcc accgcataag ccgaggacat gccaagcagc
      841 ttatttccaa gtcctgcaca cggttcaata atgatcatat tttccctcat ttcttctcta
      901 ctgcgcgtcc tgaacagtta cacttccgtt ctccaccttg tatacaatgt tgcagttttt
      961 gatggtcgac aggcgatgcg caatgatgag cattgtcatc ttaccctgca gactgtcgat
     1021 cgcctgcata accgccgcct ctgtctccgt atccagtgca gacgtcgcct cgtcgagcac
     1081 cagcacctcc ggcttgcggt acagagctct cgcaattccg attctctgac gctgtccgcc
     1141 ggacagacgc acacccgcct caccgatcac cgtgtcaagc ccctcctcga gcgactccac
     1201 aaattcatcg agctgtgcct ggcgcagtgc tgcccagaca tcctcgtcgt tgattccttc
     1261 tttttttcca aatgcaatat tgttgcggat cgtatcatcc agcatgtaga tcgtctgcgg
     1321 aatatagccg atcttatcgt gccatccgcg gtacgatgtg ctgatatcga caccgtccaa
     1381 tgtaatctgt ccctcctgcg gctttaacac cccgaggatc agatccacca tcgtggtctt
     1441 gcccgctccg gacggtccga taaacgccac cgagctgttc tttggaatcg tcacattcac
     1501 atgggacaat accggctcct ccatctccgg ataggtaaac gtcatatcgt tgacgcggat
     1561 tgcacgctca agtgccatcg ccggataatc gcgctcctcg gaaagatcaa tctccagatc
     1621 gcggatcttc gtgtactcct cgccgatctt gtggatcacg acgccgttgt gaatgatcga
     1681 cgctaagtat tccgacattt tgttggcaga aggaagcagg taaaacgccg cagccgcaaa
     1741 tacggcaagc acgccgacaa aatgggtcgg atcggactct gtcatattga tgcggaccgc
     1801 aatcgtgatc atcagacccg caatacataa cgcttccatc accggcttcg ggatggaact
     1861 taaaaatgca ttctgcttga tgacgtaggt gtaaagtccg ttctgcttgc ggaactcttc
     1921 ctcaaaataa ctctcacggt ttgcaatctt gatctccttg attccgccga atgcctgctg
     1981 catgcactga ataatgttcg cattgtattt ccggcgctca tcaccgaagc gcgccagcgt
     2041 gcgctttaac tttttcatga aaagcagcac catcagcatc atcgcaagtg ccacaccaag
     2101 cgtgataatc ggatctgtca ctaccagaaa gataattaaa attgtgcttg tgcacagttc
     2161 cgctgccagc tgcagcatat ttaataccgc tgcatagaac atctccggat cattgttgat
     2221 gtcgcgcatc agctccgcac tgttatggtt caggaaaaac gtatagtcct tgctgatata
     2281 gtacttcatc agacgatcct gcatctccct tttcccgtag taggaaaaac gatactgcat
     2341 attattcata aaaataataa acgcattttt tgccagatag acaatgacaa ttccgatcgc
     2401 tatcgccgtg atcagctcgt tgggattctc catccccatc cactcgtaaa atgccgccac
     2461 atagcggttc gcaagcagtt cctccggcgc tgtcaccgcc tggataaacg gcagaagcac
     2521 ggacacgccg agcagactga ctcccgaatt gatcagaatg agaaatgcca taccaaacaa
     2581 ctttctcttc tgctttccat caaacacctt aaagacgctc tttaccaaat tcataaaaca
     2641 atccactcct tcaaacgtat ctgt
//
LOCUS       DBMDQJ010000328         2659 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13661_length_2659_cov_1.247850, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000328 DBMDQJ010000000
VERSION     DBMDQJ010000328.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_2659_cov_1.247850"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="ACZO9R_07350"
     CDS             complement(<1..401)
                     /locus_tag="ACZO9R_07350"
                     /inference="COORDINATES: protein motif:HMM:NF016852.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HIR0912916.1"
                     /translation="MLEDHKQLRKLQLEQYKILQSVNDLCSKYGIEYYLLYGTLLGAV
                     RHSKFIPWDYDIDIAMERTQFNRFKSVVQELPDWLEVWDICYSSIEYAGLSRVVKPKD
                     CVFGDIHIDIFIIDNARITSNICTKINGALGR"
     gene            complement(424..1332)
                     /locus_tag="ACZO9R_07355"
     CDS             complement(424..1332)
                     /locus_tag="ACZO9R_07355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006427187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912917.1"
                     /translation="MPMNKTADPILTIFTPAYNRAHTLPRTYESLCRQSCKDFIWLVV
                     DDGSTDNTAALVQQWQSRDNGFEIQYIYKENGGMHTAHNVAYANIHTELNTCIDSDDM
                     LADGAVEKILSKWAEVKDKGYAGLVGLDADFTGNVIGKGFPVNMTETTISGYYAAGGA
                     GDKKLVYRTDVINAYPEYPVFDGEKYVSLSYKYLLIDQDYKLAVLDDVLCNVEYQPDG
                     SSNNMFRQYLKNPKGFAFWRTVKMQYPESFKRLVMDCIHYCSSSQIAGNRHYIKESPR
                     KLLTVLCTPAGWALTAFINKKVGLLR"
     gene            complement(1310..2437)
                     /locus_tag="ACZO9R_07360"
     CDS             complement(1310..2437)
                     /locus_tag="ACZO9R_07360"
                     /inference="COORDINATES: protein motif:HMM:NF024831.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HIR0912918.1"
                     /translation="MDKPIRVLHVIGIMNRGGAETMIMNLYRHIDRSKVQFDFVENSS
                     EPAIFDEEIISLGGRIYRCPHYNGKNHFTYVKWWNEFFQAHSKEYPIVHGHLGSTAAI
                     YLAIAKKYGVYTIAHSHNTRTCINLNEVVYAIYAFPTRYIADQLFSCSRAAGISRYGK
                     RRGGNKEQCQIVNNAIPTEMFSFLAERRKLVRNQLGLEKRLVVGHIGRFNPQKNHPFL
                     LAIFAALLKKEPNAVLLLVGGGEDMPKMQAKVRELGIADHVRFMGVRSDVADLMQAMD
                     VFAFPSLYEGLPVTMVEVQASGLPCIISDKVPPECILTEGLVDVMPLSAGAEAWADAI
                     LEKRNTPRTDRSAEIAAHGFDITTEAVKLQEFYLNAYEQNC"
     gene            complement(2449..>2659)
                     /locus_tag="ACZO9R_07365"
     CDS             complement(2449..>2659)
                     /locus_tag="ACZO9R_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912919.1"
                     /translation="RKLYPANLFENLKAIEFNGKQYMCAADPDTMLRIEYGDYMQLPP
                     EEERVWKHHPILIDFEHNYEELMQG"
BASE COUNT          663 a          712 c          574 g          710 t
ORIGIN      
        1 cgtcccaacg ctccattaat tttagtacat atatttgaag ttatacgagc gttatcgatt
       61 atgaatatat ctatgtgaat atctccaaat acgcagtcct taggctttac aactctcgac
      121 aatccggcat actctataga gctataacat atatcccaca cctccaacca gtctggaagt
      181 tcttgaacaa cgctcttaaa tcggttaaat tgcgttcttt ccatcgcgat atctatgtca
      241 taatcccacg ggataaactt cgagtggcgc acagcaccca gcagagtacc atacagcaaa
      301 tagtactcga tgccgtattt tgagcacagg tcattcacgc tttgtaaaat cttgtactgt
      361 tccagttgca attttcgtaa ttgcttatga tcttcaagca tagctacacc tctgagtttt
      421 attctaccgc aacagtccta cttttttgtt tataaatgca gtcagcgccc atcctgccgg
      481 tgtacacagc accgtcagca gcttgcgggg ggattccttg atatagtgcc ggtttccggc
      541 aatctggctg ctggagcagt agtggatgca gtccatgacc agccgcttga aggactccgg
      601 atactgcatc ttgaccgtcc gccagaaggc aaaccccttc ggatttttca ggtactgccg
      661 gaacatatta ttgctggagc cgtctggctg atattccaca ttgcacagga cgtcatccaa
      721 cacggccagc ttataatcct gatcgatcag gagatatttg taagacaagc tgacatactt
      781 ctcgccatcg aacacagggt actccggata agcattgatc acatccgtgc ggtagaccag
      841 ctttttgtcg cccgcccccc cggcagcgta gtaaccggaa atggttgtct ccgtcatatt
      901 gaccggaaag ccttttccaa taacattccc ggtgaaatca gcgtcaagcc cgacaagacc
      961 ggcatagcct ttatctttga cctccgccca tttgctcagg atcttctcca ccgcgccgtc
     1021 tgccagcata tcgtccgagt caatgcaggt gttcagctct gtatgaatat tagcgtaggc
     1081 aacattgtgg gccgtatgca tgccgccatt ttccttgtag atgtactgaa tctcaaaccc
     1141 gttatcgcgg ctctgccact gctgcaccaa cgcggctgtg ttgtccgtgg aaccatcgtc
     1201 cacaacgagc cagatgaagt ccttgcagct ttgacggcaa aggctctcat aggtgcgcgg
     1261 cagggtgtgg gcgcgattgt aggcgggggt gaaaatggtg agaatcggat cagcagtttt
     1321 gttcataggc attcaaatag aactcctgta gtttgacagc ctctgtggtg atatcgaagc
     1381 cgtgagcggc aatttcggcg ctgcggtcag tgcgaggtgt attacgcttt tcgaggatcg
     1441 cgtcagccca cgcctccgcc ccagccgata gcggcataac gtccacaagc ccctctgtca
     1501 gaatgcactc cggaggaacc ttgtcagaga tgatgcatgg cagccccgaa gcctgcactt
     1561 ccaccatggt aacaggaagt ccctcataca aagacggaaa cgcaaataca tccatggcct
     1621 gcatcagatc ggcaacatca ctgcggacgc ccatgaagcg aacatgatcc gcaatgccaa
     1681 gctcccgcac ctttgcctgc atcttcggca tatcctcacc gccgcccacc agcagaagta
     1741 cggcgttcgg ctcttttttc agcagcgcgg caaaaatggc aagcaagaaa ggatggttct
     1801 tttgcggatt aaaccggcca atgtggccga ctaccagcct tttctctaac cccaattggt
     1861 tacgtaccag tttccgtctt tctgccaaaa aggaaaacat ttccgtagga atggcgttat
     1921 ttactatttg acattgttct ttgttcccgc cgcgtctttt cccgtatctc gaaattcccg
     1981 cagctctaga acacgaaaag agctgatcgg caatatagcg tgtagggaac gcatagattg
     2041 cgtaaacgac ttcatttagg tttatacagg ttcgcgtatt atgactgtga gcaatcgtat
     2101 ataccccata tttcttcgca atcgccagat aaatcgcagc cgtactccct aaatgcccat
     2161 gcacaatagg atattctttt gaatgcgctt ggaaaaactc attccaccac ttgacatagg
     2221 taaagtgatt tttcccattg taatgcggac accgataaat tcttccgcca aggctgataa
     2281 tttcctcatc gaatatagcc ggttcgctgc tgttttccac aaagtcaaac tgcaccttac
     2341 tgcggtcaat atgacggtag agattcataa tcattgtctc cgctccaccg cgattcataa
     2401 taccgataac atgcagcact cggatcggtt tatccatacc tttgcctctt atccttgcat
     2461 aagctcctca taattgtgtt cgaaatcaat caagatcgga tgatgcttcc atacccgctc
     2521 ttcctctggc ggaagctgca tataatcgcc atactctata cgcagcatcg tatctgggtc
     2581 cgcagcacac atatattgtt ttccgttaaa ctctatcgct ttcaggtttt caaacaaatt
     2641 tgcaggataa agtttgcgg
//
LOCUS       DBMDQJ010000329         2657 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13676_length_2657_cov_1.381847, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000329 DBMDQJ010000000
VERSION     DBMDQJ010000329.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13676_length_2657_cov_1.381847"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /gene="larC"
                     /locus_tag="ACZO9R_07370"
     CDS             <1..183
                     /gene="larC"
                     /locus_tag="ACZO9R_07370"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HIR0912920.1"
                     /translation="ERAKLESTFETRKTSYGNIRIKKSSGYGIQKEKAEFEDLKSVVL
                     KNHCALSLNEVEKSLH"
     gene            complement(256..714)
                     /locus_tag="ACZO9R_07375"
     CDS             complement(256..714)
                     /locus_tag="ACZO9R_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912921.1"
                     /translation="MKQEELCRKLTEMIAETKAQKLSWRLEVQTTEGNDASEKPIEEE
                     HGTKWTIDECYASYSCNYRGKEFCMITYELLKKSGDKERSSNLVFLPPEGIRFFDLHT
                     LLPYSVEMSAVLADHIHQLWLLLMEQYKEKCPGVELIVRPGTLTIEDEGL"
     gene            complement(711..1085)
                     /locus_tag="ACZO9R_07380"
     CDS             complement(711..1085)
                     /locus_tag="ACZO9R_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912922.1"
                     /translation="MAGKNVEGQLELFSLFDSVEELEQKIKGAGLKEVQGEPVMEQCY
                     RHRDTGETAVAAYLDYNRVYVADWGRKPLLYQFDSTKEAVGYYADWLDRHASDEAAVA
                     AQRPEALQKAKAVRWENTEVEK"
     gene            complement(1122..1991)
                     /gene="metF"
                     /locus_tag="ACZO9R_07385"
     CDS             complement(1122..1991)
                     /gene="metF"
                     /locus_tag="ACZO9R_07385"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HIR0912923.1"
                     /translation="MRLTELLKTDQVTISCELFPPKQGAQLANYKNIVADMAALKPAY
                     MSVTYGATGGTSDYTVELASEVEKHQIPALAHLTCASSTKEKVASVIEELKEKQISNI
                     LALRGDIPQNADFPLPNQYRHASELIADIKAQGDFCIGGACYPEGHPEADTIFEDLDH
                     LKEKVDAGCEFLTTQMFFDNHILYNFLYKALSRGIDVPVVAGIMPVTNKAQIKRIVSL
                     SGNMVPPRFLAIVDRFGDNPAAMRQAGIAYATDQIIDLIANGVNHVHIYTMNKPDVAG
                     EILDNLSEIYVRA"
     gene            complement(2020..2523)
                     /gene="greA"
                     /locus_tag="ACZO9R_07390"
     CDS             complement(2020..2523)
                     /gene="greA"
                     /locus_tag="ACZO9R_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005609233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR0912924.1"
                     /translation="MRERLTKSDVEKIKAEIEHRKLVERKELIEAVKEARSHGDLSEN
                     FEYHAAKKDKNQNESRIRYLEKMIKTAKIISTDSAEDEVGMNNTVTVYFEEDDEEEVY
                     KIVTTVRGNSLKNLISNESPLGKALLGHRVGDRVEVRVNDDYSYFVTIRKIENTVDDG
                     TDKLRKF"
BASE COUNT          584 a          763 c          567 g          743 t
ORIGIN      
        1 gagcgcgcaa agctggaatc cacttttgag acgcgcaaga cctcgtacgg caatattcgc
       61 atcaaaaaaa gttccggcta cggcatccag aaggaaaaag cggaatttga ggatttaaaa
      121 tccgtggttt taaagaacca ctgtgctctc tctctaaatg aagtcgaaaa atccctgcac
      181 taaaaaacgt gcgggaggct gcatattctg tagtctccca cacgtttttt ctgtaatgta
      241 attaaaaacc ttgttctata gtccttcatc ttcaattgta agtgttccgg gtcttacaat
      301 cagttccaca ccggggcact tttccttgta ctgctccatc aaaagcagcc agagctggtg
      361 aatgtgatcc gcaagcaccg ccgacatctc cacactgtac ggcagtaagg tgtgaaggtc
      421 gaaaaagcgg attccctccg gcggcagaaa gaccagattg cttgagcgct ccttatcccc
      481 ggatttcttg agcagctcat aggtaatcat gcagaattct tttccgcggt agttacagct
      541 gtaagacgca taacactcat caatcgtcca ctttgttccg tgctcctcct cgatcggctt
      601 ctcggaagca tcatttccct ctgtggtctg tacttccagt ctccaggaaa gcttctgtgc
      661 ttttgtctcc gcaatcatct ccgtcagttt acggcataat tcttcctgtt tcatttctct
      721 acctccgtat tctcccagcg gactgccttc gccttctgca atgcttccgg ccgctgtgcc
      781 gctaccgccg cttcatccga cgcatggcgg tccagccagt ctgcataata cccgacagcc
      841 tcttttgtgc tgtcaaactg atacagcagg ggcttcctgc cccagtccgc cacatagacc
      901 cggttatagt ccagatacgc cgccaccgcc gtctcaccgg tgtcacggtg cctgtaacac
      961 tgttccatca ccggttcgcc ctgcacttct ttcagtccgg cgcctttgat cttctgctcg
     1021 agttcttcca ccgaatcaaa cagtgaaaat aactcaagct gtccctcaac attcttccct
     1081 gccatagcct tcctcccgca caagcccgga tcagccccag attacgctct cacatagatc
     1141 tcggacaaat tgtccagaat ctcgcccgcg acatccggct tattcatagt atagatatgt
     1201 acgtgattca cgccgttggc aatcagatcg ataatctgat ccgtcgcgta agcaattccg
     1261 gcctggcgca tcgccgccgg attatccccg aaacgatcca cgatcgccag gaaacgcggc
     1321 ggaaccatat tgccggaaag cgacacgatc ctcttgatct gcgccttgtt cgtcaccggc
     1381 atgattcccg caaccaccgg cacatcgatg cctcttgaga gtgccttgta caggaaattg
     1441 tacagaatgt ggttatcaaa aaacatctgt gtcgttagga attcacagcc cgcatccact
     1501 ttttccttca gatgatccag atcctcaaaa atggtgtccg cttccggatg tccctccgga
     1561 tagcaggcgc cgccgatgca gaaatctccc tgtgctttga tgtccgcgat cagctcactc
     1621 gcatgacggt actggttcgg aagcgggaaa tccgcattct gcggaatgtc tccccggagc
     1681 gccagaatat tgctgatctg cttctctttt aactcctcaa tcacggatgc caccttttcc
     1741 ttcgtggagg aagcacaggt taaatgtgca agcgccggaa tctgatgctt ttccacctcg
     1801 ctggcaagtt ctaccgtata gtcggacgta ccgccggtcg cgccgtaggt cacgctcata
     1861 tatgccggct tcaatgccgc catgtccgca acgatatttt tgtagttggc aagctgcgcc
     1921 ccctgtttgg gtggaaacag ctcacaggaa atcgtaacct gatcggtctt taataattct
     1981 gtcagtctca tctgctctat cctttcttct gcgtcatgcc taaaattttc tcagcttgtc
     2041 tgttccgtca tccacggtat tctcgatctt gcggatcgtg acaaaataag aataatcatc
     2101 gttgacccgc acttccacgc ggtctcccac ccggtgtcca agcagtgcct tgccgagcgg
     2161 cgactcattg ctaatcaagt tcttgagtga attgccgcgc acggtggtga caatcttata
     2221 gacttcttcc tcatcgtcct cctcgaaata cacggtgacg gtgttattca ttccgacttc
     2281 gtcctccgcg gaatccgtag atatgatctt cgctgtcttg atcatcttct ccagatagcg
     2341 gattctgctc tcgttctggt tcttgtcctt ttttgccgca tgatattcaa agttctcact
     2401 cagatcccca tgagatcttg cttcttttac cgcttcgatc aattcttttc gttccaccag
     2461 cttacggtgt tcaatctcgg ctttaatttt ttcaacgtcg cttttcgtta atctttcacg
     2521 cataatgagt cctttctgta tgaaattaaa gatttgtagt tgttggggtg atacgcttgt
     2581 aaagcaaaaa gcttctttgt agcaaggcgg acgcggaaat cagagatatt ccttcgcggt
     2641 gcagacccgc tttacct
//
LOCUS       DBMDQJ010000330         2643 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13763_length_2643_cov_1.315106, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000330 DBMDQJ010000000
VERSION     DBMDQJ010000330.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13763_length_2643_cov_1.315106"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            23..1771
                     /locus_tag="ACZO9R_07395"
     CDS             23..1771
                     /locus_tag="ACZO9R_07395"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HIR0912925.1"
                     /translation="MTTEWSLDELYKGFDDPAYGKDVAELTAQIDALATLLADAGRMQ
                     EKERTEKVLAAQSGIAQLFYQLSLYVELRQSVNTEDGTVMAENAKLMKLQAKIAPLEA
                     AAGKLLAGIRDVDALAKESGLVREHTFYLKEKQKEAKHLLSDEVEEMAASMNMSGGAA
                     WSKLQSYLTSTVKVDYQGKQITLSEVRNLAYSPEASVRKSAYEAEIAAYEKVEDAIAF
                     SLNYIKNQVTMLSEKRGYASPLAMTLEQSRMKRETLDAMMAAIDEYLPVFRKYLRKKG
                     GMLGHVNGLPWYDLFAPLGKADKTYSIEEAKQYLIDTFTKLTPEMADLMREAFENEWI
                     DFYPRKGKEGGAFCAGAMWLKQSRILTNYDGYFGSVDTLAHELGHAFHNRQIENHAPL
                     NQDYPMPVAETASTFNEVHLGKAARAEASGEELLNLLENDIKEQTQCIVDIYSRYLFE
                     TAVFEQSQNKFLMADDLKQIMLDAQKKTYGDGLDPECMHPYMWVCKGHYYSEGLSFYN
                     FPYAFGNLFALGLYSQFEKEGEAFIGKYKAMLSATPCCTIEEAGAMMGIDLTKKDFWR
                     TGLSEIAEEIEQFCTM"
     gene            1785..>2643
                     /locus_tag="ACZO9R_07400"
     CDS             1785..>2643
                     /locus_tag="ACZO9R_07400"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR0912926.1"
                     /translation="MEDLKQIMPKKLGFGCMRLPVANGNSEEIDDAVFCRMIDRYMEQ
                     GFVYFDTAYPYHNQKSEEAVRRCLVERYDRERFLLADKMPVWLVQNMEDYSEIFERQL
                     ARCGVTYFDFYLFHAMNKTRVEETEKTGGFAFVQRMKTEGRIRHIGFSFHDTAEALEE
                     ILKNHPEMEFVQLQINYYDWESENVQSRKCYEVAPKYGVPVIVMEPVKGGTLANMTGR
                     PAEILKELSPEASFASYAIRYAASLENVMLVLSGMSDEQQLIDNTAYMREFRPLDERE
                     RAAIARVVEE"
BASE COUNT          715 a          561 c          810 g          557 t
ORIGIN      
        1 aaaacattac aggaggggaa taatgacaac agaatggtcg ttagacgaac tttataaagg
       61 atttgatgat ccggcatatg gaaaggatgt ggcagagctt acggcgcaga tcgatgcgct
      121 cgccacactg cttgcagatg ccggaaggat gcaggaaaag gaaaggacag agaaggtgct
      181 tgcggcgcag agcgggatcg cacagctttt ctatcagtta tcgctctatg tggaattgag
      241 acagtccgtc aatacagagg acggcacagt gatggcagaa aatgcgaagc tgatgaagct
      301 tcaggcaaaa atagcaccgt tagaggccgc ggcaggaaag cttttagcgg gaatccggga
      361 cgtggatgcg ctggcaaaag agagcgggct tgtgcgtgag catacatttt acctgaaaga
      421 gaagcagaaa gaggcaaagc atctgctcag cgatgaagtg gaggagatgg ctgcctccat
      481 gaacatgagc ggcggcgcgg cgtggtcgaa gctgcagtcc tatctgacct ccacggtaaa
      541 agtggattat cagggaaagc agattacact gtcggaagta agaaacttag cgtacagccc
      601 ggaggcttcc gtgcgcaaga gcgcttatga ggcggagatt gccgcttatg agaaggtgga
      661 ggatgccatc gcgttttccc tgaattacat taagaaccag gtgacgatgc tcagcgaaaa
      721 gagaggctat gcttcaccac ttgccatgac gttagagcag tcgcgcatga aacgagagac
      781 tttagacgct atgatggcgg cgatcgacga atacctgccg gtattccgca agtatctgcg
      841 gaaaaagggc gggatgctcg gacatgtcaa cggcctgccg tggtatgatc tgtttgcacc
      901 gctcggcaag gcagataaga cctacagcat tgaggaggcc aagcagtatc ttattgatac
      961 attcacaaag ctgacaccgg agatggcaga tctgatgcgg gaagcgtttg agaacgagtg
     1021 gatcgatttc tacccgagaa aaggaaaaga gggcggtgct ttctgcgcgg gagccatgtg
     1081 gttaaaacag agccggattc tgaccaatta cgacggttat ttcggatcgg ttgacacgct
     1141 tgcacatgaa ctcggacatg cattccacaa ccgccagatc gagaatcacg caccgcttaa
     1201 tcaggactac ccgatgccgg tagccgagac agcatcaacc tttaacgagg tgcatctcgg
     1261 aaaggcagcc cgggcagagg catccggaga ggagcttttg aatctgctgg agaacgacat
     1321 caaggagcag acgcagtgta tcgtggacat ttactcccgc tatctgtttg agacggcagt
     1381 atttgaacag agccagaata agttcctcat ggcggacgat ttaaagcaga tcatgctgga
     1441 cgcgcagaag aagacgtacg gcgacgggct ggacccggaa tgcatgcatc cgtatatgtg
     1501 ggtgtgcaag ggacattatt acagcgaggg actgagcttt tacaatttcc cgtatgcttt
     1561 tggcaatctg tttgcgcttg gactttacag ccagtttgaa aaagaggggg aggcttttat
     1621 tgggaaatat aaggcaatgc tttcggcaac cccgtgctgc acgatcgagg aggctggcgc
     1681 aatgatggga atcgacctca caaagaagga tttctggaga acgggacttt ctgagattgc
     1741 ggaagagatt gagcagttct gcacgatgta gaaggagatg tgttatggaa gatttaaaac
     1801 agatcatgcc gaagaagctt gggtttggct gtatgcggct gcccgttgca aacggcaata
     1861 gcgaagagat tgacgacgcg gtattctgca ggatgattga ccgctatatg gagcagggat
     1921 ttgtctattt tgacacggca tacccttacc acaatcagaa gtcggaggag gcagtgcgtc
     1981 gctgtctggt ggagcgttat gacagagagc gcttccttct cgcagacaag atgccggtgt
     2041 ggctggtaca gaacatggag gactacagcg agatctttga gaggcagctg gcgcgctgcg
     2101 gcgtgactta ttttgacttt tatctgtttc acgccatgaa caagacacgg gtggaagaga
     2161 cagagaaaac gggcggcttt gcgtttgtgc agagaatgaa gacggaaggg cgcatccgcc
     2221 atatcggatt ttcgttccat gatacggcag aagcgctcga ggagatcctg aaaaatcatc
     2281 cggagatgga atttgtgcag cttcagatca attattatga ctgggagtcg gagaatgtgc
     2341 agtcccgcaa atgctatgag gttgccccaa agtatggtgt accggtgatc gtgatggagc
     2401 cggtaaaggg cggaacactc gccaacatga ccggcagacc ggcagagatc ttaaaggagc
     2461 tttcaccaga ggcaagcttt gcatcttacg cgatccgcta tgcggcatca ctggaaaatg
     2521 tgatgctggt gttgagcggc atgtcggacg agcagcagct catcgacaac acggcttata
     2581 tgagggagtt cagaccactt gatgagcggg agcgcgcagc gatcgcccgg gtggtggagg
     2641 agc
//
LOCUS       DBMDQJ010000331         2638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13793_length_2638_cov_1.709499, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000331 DBMDQJ010000000
VERSION     DBMDQJ010000331.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13793_length_2638_cov_1.709499"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /gene="ssrA"
                     /locus_tag="ACZO9R_07405"
     tmRNA           <1..123
                     /gene="ssrA"
                     /locus_tag="ACZO9R_07405"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            261..860
                     /locus_tag="ACZO9R_07410"
     CDS             261..860
                     /locus_tag="ACZO9R_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912927.1"
                     /translation="MSVEKRQKEQEKTSMVVEGFLFANEAEAAQARKEAEGVKYVKEK
                     AQMDDPEMVLRIYNKMIQQRLFDTAVGYSYLKDLQEYLQSIPFIRKEDIFPIPVQHPQ
                     VEESLRKAKRAPAPKPKTQTVVKNADYRRRYRIVRWIAFILAVCVVAMFAITATTNNT
                     TVLNYENQLINKYEAWEERLDQRQEAIEAKEKELGIMPE"
     gene            932..1618
                     /locus_tag="ACZO9R_07415"
     CDS             932..1618
                     /locus_tag="ACZO9R_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0912928.1"
                     /translation="MDKIRILVVDDESRMRKLVRDFLVREGYEVLEAGDGEEALDLFY
                     REKDIALIILDVMMPKRNGFEVCREIRETSKVPIIMLTAKGEESDELNGFELGVDEYI
                     SKPFSPKILVARVGAILRRSGKTQDEEAAVSMGGIVLDKAAHMVTVDGERIDLSYKEF
                     ELLAYFMENAGIALSREKILNSVWNYDYFGDARTIDTHVKKLRAKMGDKGKYITTVWG
                     MGYKFEVTEE"
     gene            1634..>2638
                     /locus_tag="ACZO9R_07420"
     CDS             1634..>2638
                     /locus_tag="ACZO9R_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HIR0912929.1"
                     /translation="MKGLFDKKRQGRGRRPKEGGLTREIAGIIIGLVAGTVALCWVLN
                     TVFLERYYVMNKENVLLSGFTTIDAASEDGSLDDSSFDVTFDNLCANGNITVMVISPD
                     RTIVRSSVNDTQRMLIEFTDIIFGGTQNETTVLAQAEDYVIQRRTDTRLDAEYLVLYG
                     TLSNGNLVLMRSALESIRESAKTSNQFLLYVGILAIAVSTVVSVFISKRITDPILELT
                     EISKHMTQLEFEVCYTPKGKRRNEIDELGTHMNEMSQALEHTISELKSANNQLLLDIE
                     KKTQIDRQRKEFLSNVSHELKTPLALIQGYAEGLKECINDDAQSRDFYCEVIMDEADK
                     M"
BASE COUNT          750 a          537 c          797 g          554 t
ORIGIN      
        1 ctaccaaaac cgtaagggtg tctgttcccg gacggcagag ggaatgtcaa aggacagact
       61 aagctcgtag aaggacaggg gattattctt cggacacggg ttcgattccc gtctggtcca
      121 tcttaaaaag cgccatgcag ggcgcttttt tttatgggaa aatgcggaat ctttacatct
      181 ccctgacttg caattcacac tgctatttcc tataataaga gcaaaatcac agcagaaacg
      241 gaagagatga ggatcgaaga atgtctgtag aaaagagaca aaaagaacag gaaaagacct
      301 ccatggtcgt ggagggattt ttatttgcaa atgaggcgga agccgcgcag gcgagaaaag
      361 aggcggaggg cgtgaaatac gtcaaggaga aagcccagat ggacgaccct gagatggtac
      421 tccgcattta taataaaatg atacagcagc ggctgtttga cacggcagtc ggctattctt
      481 atctgaagga tctgcaggag tatctgcagt cgattccgtt tatccggaag gaagatattt
      541 tcccgattcc ggtgcagcat ccgcaggtgg aggagagtct ccgcaaggcg aagcgcgcgc
      601 cggcaccaaa gccgaagacg cagaccgtgg taaaaaatgc ggactacagg agacgctacc
      661 gcattgtgcg ctggatcgcc tttattctgg cagtatgtgt tgtggcgatg tttgcaatta
      721 cggcgacgac caataatacg acggtgttaa actacgagaa tcagctgatc aacaaatacg
      781 aggcgtggga ggagcggctc gaccagaggc aggaagcgat cgaggcgaag gaaaaagagc
      841 ttggaatcat gccggaatag agcggaaaat acacagattt ttcataagta gctgttagaa
      901 tgacgataaa aagcggaaag gatacgggaa catggataag atcagaattc tggtggtgga
      961 tgatgagagc agaatgcgca agctggtgcg ggatttcctt gtgagagagg gatacgaggt
     1021 gcttgaggcg ggagacggcg aggaagccct cgatttattt taccgggaaa aagacatagc
     1081 gctgattatt ttagacgtca tgatgcccaa gcgcaacgga tttgaagtgt gccgggagat
     1141 ccgggagaca tcgaaagttc cgattattat gctgacggcg aagggcgagg agagcgacga
     1201 gctgaacgga tttgaactcg gtgtggacga atatatttcc aaaccttttt ctccgaagat
     1261 actggttgcg cgtgtcggag ccattctgcg ccggagcgga aagacgcagg atgaagaagc
     1321 ggcggtctcc atgggaggaa tcgttctgga caaggcagcc cacatggtga cggttgacgg
     1381 agagcggatc gatttaagct acaaggagtt tgaactgctg gcatacttta tggaaaatgc
     1441 ggggattgcg ctctcacgcg agaagatatt gaacagcgtc tggaattacg attactttgg
     1501 cgatgcgaga acgattgata cgcatgtcaa gaagctgcgt gccaagatgg gagataaggg
     1561 aaaatatatc acgacggtct ggggcatggg ctacaaattt gaagtgacgg aagaataatg
     1621 gaagtgacgc ggaatgaagg gactttttga taaaaagaga caggggcgcg gcagacgtcc
     1681 gaaagagggc ggactgacca gggaaatcgc cggaattatc atcggtctgg tggcgggaac
     1741 cgtggcgctc tgctgggtgc ttaacacggt atttctggag cgctactatg tgatgaacaa
     1801 agaaaatgta ctgctgtccg gatttactac gattgatgcg gcaagcgagg atggaagcct
     1861 tgacgacagc tcgtttgatg tgacatttga caatctctgc gcaaacggca atatcacggt
     1921 catggtgatc agcccggacc gcacgattgt cagatcctcc gtgaacgata cacagcgtat
     1981 gttaatcgag tttacggaca ttattttcgg tggtacgcag aatgagacca cggtgctggc
     2041 gcaggcggag gactatgtga tccagagaag aacggacacc aggctggatg cggaatatct
     2101 ggtactgtac ggaactctct caaacggcaa tctggtgctg atgcggtcgg cgttagagag
     2161 cataagggag agcgccaaga cgtcgaacca gttcctgctg tacgtcggga tcctggcgat
     2221 tgcggtgagc acggttgtgt cggtcttcat ctctaagaga atcaccgatc cgatcctgga
     2281 actgaccgag atttccaagc atatgaccca gctggaattt gaggtctgct atacgccgaa
     2341 aggaaagcgg cgcaatgaga tcgatgaact cggcacgcac atgaacgaga tgtcgcaggc
     2401 gctcgagcat acgatctcgg aattgaaaag cgcaaacaac cagctccttt tagacattga
     2461 gaaaaagacg cagatagacc gccagcgcaa ggaatttctc tcgaatgtat cgcatgaatt
     2521 aaagacgccg cttgccctga tccagggata tgcggagggc ttgaaggaat gtatcaacga
     2581 tgacgcacag agccgggatt tttactgtga ggtcatcatg gacgaggcgg ataagatg
//
LOCUS       DBMDQJ010000332         2630 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13852_length_2630_cov_1.371688, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000332 DBMDQJ010000000
VERSION     DBMDQJ010000332.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13852_length_2630_cov_1.371688"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1217
                     /locus_tag="ACZO9R_07425"
     CDS             <1..1217
                     /locus_tag="ACZO9R_07425"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013271628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912930.1"
                     /translation="AAPMFALSSFLQAFVRNDGAPKLAMAGVISGGVTNVILDYVYIF
                     IMKWGMGGAALATATGTTLTVLILASHFFSKDNHLKLEFSDMWRRVPEVLGNGLASFI
                     LEISNGFVMLLFNRQLLAYVGEMGITVEGDMPNQEFMQMKESFDSMSAQVKYLFDSMY
                     SEKLARKDAQIQALQAQINPHFLNNTLEMMNWQARMSGDTVVSKMIESLGTVLDYRMN
                     RANVKEIHLAEELQCIDAYFYIMSMRFGQRLSIERKIDDDLLYLMVPPLILQPIVENA
                     IVHGVETVKNGVIRLHVYHDDENVYLKVRNTGKKMTEEELERIHAILEGDESKLPKNT
                     GRHTSIGIQNVNRRIRLVYGDEYGLSIEQEEDCITVSTITIPYVDHEDEISLKERSEA
                     QNELKNMARLNK"
     gene            1318..>2630
                     /locus_tag="ACZO9R_07430"
     CDS             1318..>2630
                     /locus_tag="ACZO9R_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0912931.1"
                     /translation="MKKKVLSMMLAGAMTVSMLAGCGAGSDEAATTDNTAADTKTETA
                     GTTEAGGGTAEASGNPVTLRTVSMFGGTDPNKETYEAINKEFMEQYDYITIEDNSQTS
                     DEEWKAAVNADFSVGNEPDVIQFFTDATADSIIATDKLVSIEDIRAEYPDYAADTLDS
                     ALQAAANTDGVERAVPTTGYWEGLYCNKDLFDQYDLELPTDWASMETAIETFKENDII
                     PIACSLNNVPHYWIEFLMLYASGVDEYASIPTSAPEGWVKGLEMFKTLRDMGAFPEDT
                     DTVDDAYTGQLFRDKKAAMQLDGSWYAGNIEDTDNTVVIAFPGVPDQKAEAGSLVGGI
                     SSGFYITKKAWEDPDKRDAAVKFVMAHTCQAGVQRYWEATGAVSQAAVEVTPVEGATP
                     FAQSIAEYTSNATAIVAPTDSRIGDAYKTLVVEIVKVSTGAMSAE"
BASE COUNT          736 a          582 c          751 g          561 t
ORIGIN      
        1 cagcggcccc catgtttgcc ctgtcttcat tccttcaggc attcgtccga aacgatggtg
       61 cgccgaagct tgccatggcg ggcgtgatca gcggaggtgt caccaatgtg atcctggatt
      121 atgtatacat ttttatcatg aaatggggaa tgggaggtgc ggcgctggcg acggccaccg
      181 gaacgacact cacggtgctg atcctcgcaa gccatttttt ctcgaaggac aatcacttga
      241 agctggaatt tagcgatatg tggagacggg tgccggaggt gctcggaaat gggctcgcaa
      301 gctttattct ggagatctcc aacggcttcg tgatgctgct gtttaaccgc cagctcctcg
      361 cttacgtggg agagatggga atcacggtcg agggagacat gcccaatcag gaatttatgc
      421 agatgaagga gagcttcgac agcatgtccg cgcaggtgaa gtatctgttt gattccatgt
      481 acagtgaaaa gcttgcgcgc aaggatgcac agattcaggc gcttcaggcg cagatcaatc
      541 cgcatttcct gaacaatacg ttagagatga tgaactggca ggcacggatg tcgggagata
      601 cggtcgtgtc taaaatgatt gaatcgctgg gaaccgtgct tgattaccgg atgaaccgcg
      661 ccaatgtaaa ggagattcac cttgcggagg aactgcagtg cattgatgcc tatttttaca
      721 ttatgtccat gcggttcggg cagagactct cgatcgagcg caagattgac gatgatctgc
      781 tctatctgat ggtgccgccg ctgatcttac agccgattgt ggaaaatgcg attgtgcatg
      841 gggtagagac ggtaaaaaac ggcgtcatcc ggcttcacgt ataccacgat gatgaaaatg
      901 tctacttaaa agtgcgcaat acgggcaaaa agatgacgga ggaggagctg gagcggatcc
      961 atgctatatt ggagggcgat gagagcaagc ttccgaagaa taccggacgc cacacctcca
     1021 ttgggattca gaacgtgaac cgcagaatcc gcctggtata tggggacgaa tacggtctgt
     1081 cgatcgagca ggaggaagac tgcatcacgg tttcgacgat tacgataccg tacgttgacc
     1141 atgaggatga aatatcgtta aaagagagaa gcgaagcgca aaatgagtta aagaacatgg
     1201 caagattgaa caaataatgt caaataggtc taatgccttt tgtgagttct gctcgtataa
     1261 tatcagtata gcaaatgaaa aacatttgca ggaacaaaaa caaggagggt tttgactatg
     1321 aagaaaaagg tattatccat gatgctggct ggcgcaatga cagtgtccat gctggcagga
     1381 tgcggggcgg gctccgatga ggctgcaacg acagacaaca cggcagccga cacaaagaca
     1441 gagacagccg gaacaacaga agcaggcggg ggtactgcag aagcatccgg aaatccggtg
     1501 acattaagaa ctgtgtcgat gttcggagga accgatccga ataaggagac ctacgaagca
     1561 atcaacaaag agtttatgga gcagtatgac tacataacga tcgaagacaa ttcacagaca
     1621 tccgatgagg aatggaaagc agcagtcaat gcggacttct ccgtaggcaa tgagccggat
     1681 gtcatccagt tcttcacgga tgcaaccgca gacagcatca ttgcaacgga taagctggta
     1741 tcaattgaag acatccgcgc agaatatccg gactatgcgg cagacacact ggattccgca
     1801 ttacaggcag cggcaaacac cgacggtgtt gagcgtgcgg ttccgacaac cggatactgg
     1861 gaaggcttat actgcaacaa ggatctgttc gaccagtacg atctggaact tccgacagac
     1921 tgggcttcca tggagacagc gattgagacg ttcaaggaga acgacatcat tccgattgcc
     1981 tgctcgttaa acaacgtacc gcattactgg attgagttcc tgatgttata tgcatccggc
     2041 gtggatgagt atgcttccat tccgacgagc gcgccggaag gatgggtaaa gggtcttgag
     2101 atgtttaaga cattaagaga tatgggtgca ttcccggagg ataccgacac cgttgatgat
     2161 gcatacacag gccagttatt ccgcgacaag aaagctgcca tgcagttaga cggttcctgg
     2221 tatgcaggaa acatcgagga taccgacaac accgttgtga tcgcattccc gggtgttccg
     2281 gatcagaaag cagaggcggg ctctttggta ggcggtatct cttccggatt ctatatcaca
     2341 aagaaggcat gggaagatcc ggacaagaga gatgcagcag tgaaattcgt catggcacac
     2401 acctgccagg caggcgttca gagatactgg gaagcaaccg gcgctgtgtc ccaggcagct
     2461 gttgaggtaa caccggtaga gggtgcaaca ccgtttgcac agtctatcgc agagtacaca
     2521 agcaacgcaa cggcgatcgt ggcaccgaca gattccagaa tcggcgatgc atacaagaca
     2581 cttgtggtag agattgtcaa ggtatctaca ggtgctatgt cggcggaaga
//
LOCUS       DBMDQJ010000333         2627 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13872_length_2627_cov_1.686461, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000333 DBMDQJ010000000
VERSION     DBMDQJ010000333.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13872_length_2627_cov_1.686461"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="ACZO9R_07435"
     CDS             complement(<1..978)
                     /locus_tag="ACZO9R_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR0912932.1"
                     /translation="MTEEIASQEIDFMEYGDTQINTIDMERGQRKDLIGKMPVGIAVV
                     RGGNELYIEMVNREFLHAEGYDREELTGNTPFVEYLYRDDTGVFEDAIEVCRELRTTE
                     EIELRIRTKSGGVCWELMRCRLYYYRDAVPYYILASWNIDKRKNLEEELRLMEEQYSM
                     LEEVTDEFPLEYDVAQRRFRVPRKYRGNGQPGTAGRRYMDYEEMLADICEEDRAAYAR
                     VLEAASRDVRTGSIDYRMNVAAAGEEPVYVWYRTIYRSIVGDNGQIIRIIGRSYDVSS
                     DRRIQEELSEEAKRDPLTRLYNKVAASGEVERILKSEPEKQHVLFLIDID"
     gene            1168..>2627
                     /locus_tag="ACZO9R_07440"
     CDS             1168..>2627
                     /locus_tag="ACZO9R_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR0912933.1"
                     /translation="MISTSNITLRIGKKALFEDVNIKFTEGCCYGLIGANGAGKSTFL
                     KILSGQLEPTSGEVIITPGQRLSFLQQDHFKYDEYTVLDTVIMGNKRLYDIMKEKDAI
                     YMKEDFTDEDGIRASELEAEFADMDGWNAESDAATMLNGLGIPTEDHYTLMADMPSAL
                     KVKVLLAQALFGNPDILLLDEPTNHLDLDAISWLEEFLINFENTVIVVSHDRYFLNKV
                     CTNIADIDYGKIQLYAGNYDFWYESSQLLVKQMKEANKKKEEKIKELQEFISRFSANA
                     SKSKQATSRKRALEKIQLDEIRPSSRKYPYIDFRPNREIGNEVLTVEGITKTIDGVKV
                     LDNVSFIVGHDDKIAFVGSNELAKTTLFKILAGEMEPDSGTYKWGVTTSQSYFPKDNT
                     EIFDTDLLIVDWLTQFSEIKDATYVRGFLGRMLFAGDDGTKKMRVLSGGEKVRVLLSR
                     MMIMGSNVLILDEPTDHLDMESITALNNGLIKFPGV"
BASE COUNT          657 a          740 c          579 g          651 t
ORIGIN      
        1 atcaatatcg atcaggaaca gcacatgctg tttttccggc tcacttttta aaatccgttc
       61 tacctctccg gatgccgcca ccttattata aagccttgtc agcgggtcgc gcttcgcctc
      121 ctccgacagt tcctcctgaa tcctgcggtc ggagctgaca tcgtagctgc gtccgatgat
      181 gcggataatc tgtccgttgt ccccgacaat gctccggtaa atcgtccggt accacacata
      241 tacaggttcc tccccggcag ccgccacatt catccggtaa tcaatacttc ctgtccgcac
      301 atccctgctg gctgcctcca gcaccctggc atacgcggca cgatcctcct cgcagatatc
      361 cgcgagcatc tcctcataat ccatatatct gcgccctgcc gttcccggct gcccgttccc
      421 gcgatatttg cgcggcaccc tgaaacgccg ctgtgcaacg tcgtactcta gcgggaattc
      481 atcggtgacc tcttctaaca tgctgtactg ctcctccatc aggcgcagtt cttcctccag
      541 attcttccgc ttgtctatgt tccagcttgc caggatatag taaggtaccg cgtcccggta
      601 ataatacagt ctgcaccgca tcagttccca gcagacgccg ccgctcttcg tacggatgcg
      661 cagttcaatc tcctccgtgg ttcttagttc ccggcacact tcgatggcat cctcgaacac
      721 accggtatcg tcccggtaca aatactccac aaacggcgta tttccggtca gttcctccct
      781 gtcatacccc tctgcatgca aaaattcccg gttcaccatc tcaatataca gctcattccc
      841 gccgcggact accgctattc cgaccggcat cttgccgatc agatcttttc tctgcccccg
      901 ctccatgtct attgtattta tttgcgtatc cccatactcc ataaaatcaa tctcctgaga
      961 tgcaatttcc tctgtcatac ttaagataac tccgattgcc ttcccgtaca ttatatcaaa
     1021 tttttaacga aatccaaaca atttgtcaca gcgtgaaagc cctttttaat aagaagttcc
     1081 ggttgcattt ctccgtattt tttagtatga tacgaattgg tgcaataaaa gcaggtgatt
     1141 tacaagaaaa gttaggagaa cataaatatg atcagtacaa gcaacatcac tttacgaatc
     1201 ggaaaaaaag ctctctttga agatgtcaac atcaaattca cagagggctg ctgctacggt
     1261 ctgatcggtg caaacggcgc cggaaaatct acgttcctca agattctgtc cggccagctt
     1321 gaaccgacga gcggagaagt tatcatcacg ccgggtcagc gtctttcctt tttgcagcag
     1381 gatcatttta aatatgacga gtacacggta ctcgataccg ttatcatggg caacaagcgt
     1441 ctctacgata tcatgaaaga aaaagacgcg atctacatga aagaggattt cacggacgag
     1501 gacggtatcc gcgccagcga acttgaagcg gagttcgcag atatggacgg ctggaatgcc
     1561 gagtccgatg cagccaccat gttaaatggt cttggcatcc cgacggagga tcactacaca
     1621 ctcatggcgg atatgccaag tgccttaaag gtcaaggttc tccttgcaca ggcgctcttc
     1681 ggcaacccgg acattcttct tttggatgag ccgaccaacc atctggatct cgatgcgatc
     1741 agctggttag aagaattctt aatcaacttt gagaacacgg tcatcgttgt ttcccacgac
     1801 cgctacttct taaacaaagt ctgcacaaac attgcagata tcgactacgg aaagatccag
     1861 ctctacgccg gcaactatga tttctggtat gagtccagcc agcttctcgt aaagcagatg
     1921 aaggaagcca acaagaaaaa agaggagaag atcaaggagc tgcaggaatt catctcccgt
     1981 ttctccgcga atgcttccaa atccaagcag gcgacttcca gaaagcgtgc cctggaaaag
     2041 attcagctcg acgagatccg tccgtccagc cgtaaatacc cgtatatcga tttccgtccg
     2101 aaccgtgaga tcggcaatga agttctgacc gtcgaaggca ttaccaagac catcgacggc
     2161 gtcaaggtgc tcgacaatgt ctcctttatc gtcggacatg acgacaagat cgcgtttgtc
     2221 ggaagcaacg agctggcaaa gacaacgctc tttaagattc ttgccggtga gatggagccg
     2281 gattccggca cctacaagtg gggcgtgacg accagccagt cctacttccc gaaggataac
     2341 accgagatct ttgataccga tcttctgatc gttgactggc tgacacagtt ctccgagatc
     2401 aaggatgcaa cctatgtccg cggtttcctc ggacgtatgc tgttcgccgg agatgacggt
     2461 accaagaaaa tgcgtgtcct ctccggtgga gaaaaagtac gtgtcctgct ttcccgcatg
     2521 atgattatgg gaagcaacgt gctgatctta gatgaaccga ccgaccatct ggatatggag
     2581 tccatcaccg cattaaacaa cggactcatc aagttcccgg gcgttat
//
LOCUS       DBMDQJ010000334         2625 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13884_length_2625_cov_1.589540, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000334 DBMDQJ010000000
VERSION     DBMDQJ010000334.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13884_length_2625_cov_1.589540"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="ACZO9R_07445"
     CDS             <1..329
                     /locus_tag="ACZO9R_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912934.1"
                     /translation="YFAPEAVDVRQIADVLDGTCELEIWQEAGVLEIMYGGESSMDME
                     EGTIHPKDQVTAAFAEEHDCDRVYLVTFSAEEYEKVLPVMRHILQECGGIFCGDTEDF
                     KPILTE"
     gene            434..1303
                     /gene="aroE"
                     /locus_tag="ACZO9R_07450"
     CDS             434..1303
                     /gene="aroE"
                     /locus_tag="ACZO9R_07450"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HIR0912935.1"
                     /translation="MDSRISGHTGLLALIGSPVGHSGSPAMYNYSFERLGLDYVYVAF
                     DIKVEEVADAIAAMRTFKMRGCNVTMPCKTEVVRYMDELSPAAQIIGAVNTIVNDNGR
                     LTGHITDGEGFVHNLKDHGIDIKGKKITVAGGGGAATAIQVQCALDGAREISIFNIKD
                     AFFERTLKTAEKIRAAVPSCVVNVYDIADTARMTAEIASSDIFANATIVGMKPMENES
                     VVKDTSAFRPGLVVADAVYNPEETRLLREAKEAGATCIGGKGMLLWQGVAAFKLYTGK
                     DMPVEEVKERFFS"
     gene            1321..1833
                     /locus_tag="ACZO9R_07455"
     CDS             1321..1833
                     /locus_tag="ACZO9R_07455"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR0912936.1"
                     /translation="MKNLYLIGFMGAGKSTIARALAKKCPSKRIEMDQLIEEQQGMAI
                     TDIFAAHGENYFRDLETELLRSFSESTGYVVSCGGGSVLRDENAALMKENGCIVFLRA
                     TPETIYERVKDSTNRPILNGNMNVEYIRELMEKRRPRYEAVADICVDTDGKDVDAISE
                     EILKAVEDRS"
     gene            1849..>2348
                     /locus_tag="ACZO9R_07460"
     CDS             1849..>2348
                     /locus_tag="ACZO9R_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912937.1"
                     /translation="MNFEFIRECRLESDELQAMYDNVLQELERAEHYYWRKPQECGII
                     LRQTTERICRIYNTYYQIGYPGNASLEEFLCYTDENEHNVMVSRFLSVVRKEQRDRLN
                     KLRVLGDDCIWGEEAPDQGMTFEDRMGQNARHMMETMMEVTKDMCEKINKRDDVFDEF
                     FLEEALP"
     assembly_gap    2349..2358
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          700 a          493 c          835 g          587 t
ORIGIN      
        1 tttattttgc accggaggca gtggatgtgc ggcagattgc agatgtgctc gatggaacct
       61 gcgagttgga gatctggcag gaagcaggcg tgcttgaaat tatgtacggt ggagaatctt
      121 ccatggatat ggaggagggg acgatccatc ctaaggatca ggtgactgcg gcatttgcag
      181 aggaacatga ctgcgacaga gtgtatctgg tgacattttc tgcggaggaa tatgaaaagg
      241 tactgcctgt aatgcggcac atcttacagg aatgcggcgg catattctgc ggcgatacgg
      301 aagattttaa gccgatactt acagagtaga aaagggatgc ttttttgctt tagtgtattg
      361 aaatgataag aaaaaacagc tatgatactg ttattggcgc agagaaaaaa cggacgccgg
      421 agcgggagga aatatggaca gcagaatttc aggacacaca ggattattgg cattgattgg
      481 ttcgccggtc ggacattcgg gttcgccggc aatgtataat tacagcttcg agcggctggg
      541 actggattat gtgtatgttg cttttgatat caaggtggag gaagtggcag acgccattgc
      601 cgccatgcgc acatttaaga tgcgcggctg caatgtcacc atgccgtgca agacggaggt
      661 tgtcagatac atggatgagc tgtctccggc ggcacagatc atcggagcgg tcaatacgat
      721 tgtaaatgac aacggcaggc tgaccggtca tattactgac ggcgagggat ttgtgcataa
      781 tctgaaggat cacgggatcg atattaaggg caagaagatc acggttgcag gcggaggcgg
      841 tgccgctact gccattcagg tgcagtgcgc gcttgacggt gcaagggaga tttccatttt
      901 taatatcaag gatgcttttt ttgagagaac gctgaaaacg gcggagaaga tccgtgcggc
      961 ggttccgtcg tgcgtggtaa atgtctatga tattgcggat actgcgcgga tgactgcgga
     1021 gatcgcgtcc agcgatattt ttgcaaatgc aacgattgta ggcatgaagc cgatggaaaa
     1081 tgagagtgtg gtaaaggaca cttctgcatt ccgaccgggg ttagtggtgg cggatgccgt
     1141 ctacaacccg gaggagacga gattgctccg ggaggcgaag gaagccggag caacctgtat
     1201 cggcggcaag ggaatgctgc tgtggcaggg cgtggctgcc tttaaactgt ataccggaaa
     1261 agatatgccg gtcgaggaag tcaaagagcg tttttttagc tagaaaggtg aaactgcggc
     1321 atgaagaatt tatatctgat cggctttatg ggcgccggga aaagtaccat tgcgagagcg
     1381 cttgcgaaaa aatgtccgtc gaagcgaatc gagatggatc agctcatcga ggaacaacag
     1441 gggatggcaa tcacagatat ttttgccgca catggggaga attatttccg cgacctggag
     1501 acagagttgt tgcggtcttt ttcggagagc acaggatatg tggtatcctg cggcggaggc
     1561 agtgtactca gggatgagaa tgcggcgctg atgaaggaga acggttgtat cgtgttcctt
     1621 cgggcgacgc cggagacgat ctacgaaaga gtcaaggaca gtacgaaccg cccgatttta
     1681 aacgggaata tgaatgtgga atatatccgg gaactcatgg agaagcgccg gccgcgctac
     1741 gaagcggtgg cggatatctg cgtggacacg gacggcaagg acgtggatgc catcagtgag
     1801 gagatcctga aagcggtaga agacagatca taagaggggc agcgggagat gaattttgag
     1861 tttataagag agtgcaggct ggagtcggac gagcttcagg cgatgtatga caatgttctg
     1921 caggaactgg agcgcgcgga gcattattat tggagaaaac cgcaggaatg cggcatcatt
     1981 ctgcgccaga caacagagcg gatctgtcgt atctataata catattatca aatcggctat
     2041 ccggggaacg cttcgctgga agagtttttg tgttatacgg acgaaaacga gcacaacgtg
     2101 atggtgagcc gttttctgag cgtcgtccgc aaggagcagc gcgaccggct gaataagctg
     2161 cgtgtgctgg gggatgactg tatctggggc gaggaggcac cggatcaggg gatgactttt
     2221 gaagaccgca tggggcagaa tgcaagacat atgatggaga ccatgatgga ggtcaccaag
     2281 gatatgtgcg agaagatcaa caagagggac gatgtgtttg acgagttctt cctcgaggag
     2341 gctctgccnn nnnnnnnnct gccggagatg aaggaagagg caggaaaaga ggcacttgcc
     2401 gctgcggaga taacaaccag tgcagagaat acgaaaaaat ctttgtttgc cagaattttc
     2461 cacaggtgag gataaatgaa aaaatcagta tcgatcgttt atggagtgct gcttgtcatc
     2521 ggatttattc tgatggcgtt gagctttgcg tttgcaggta aaaagaatac aagctttgcg
     2581 gaggattcca gacgaatcga actgcagccc gatcgcgtgg tggaa
//
LOCUS       DBMDQJ010000335         2620 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13909_length_2620_cov_1.502183, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000335 DBMDQJ010000000
VERSION     DBMDQJ010000335.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13909_length_2620_cov_1.502183"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(248..982)
                     /locus_tag="ACZO9R_07465"
     CDS             complement(248..982)
                     /locus_tag="ACZO9R_07465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020034.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0912938.1"
                     /translation="MKDGQKQYRILRIDEPDFGCEGRDDGEAAYDEITLVNEQGETCL
                     VRMEEQQVWNMGLDEGMLVWVGKDGQIRGRCKRMDISRWSEKYQVRVLTEQDVGAIYE
                     LSRKNNLYYEYCPPYVTRDSILSDLVALPPGKLPDDKYYVGFFRGETLLAQMDLIADY
                     PERAMAYIGLFMMEASVQQAGIGTRIVEDLCRHLAEEGIRTVRLCWVKGNPQAEHFWR
                     KNQFAPIRETQSMSGQTVVLAERRLK"
     gene            complement(1026..1889)
                     /locus_tag="ACZO9R_07470"
     CDS             complement(1026..1889)
                     /locus_tag="ACZO9R_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6033 family protein"
                     /protein_id="HIR0912939.1"
                     /translation="MSLIPDYANAVGSAMSYNRKAADTKGKEKTDGNASSEKTGKANK
                     ADKTDRTGKTGTVTGRTIGNPKLSEKAAKYYEELKRKYSNMDFILVSEDQKEKARTQA
                     GSYANASKMVVLIDEDKIERMAEDENYRRQYEAIIANAGSGIAQMKSSLEASGTKVKG
                     YGMQVNDGGTATYFAVLQKSSDAQKERIEKKREKAAEEKKAEARRAQKDAQEKRIRDG
                     KNQRDEHAAERVNKTDEASEADWENTVTITASSIEELLKKISDYQQNERMNTVQTKEE
                     RLVGGHIDYKG"
     gene            2048..>2620
                     /locus_tag="ACZO9R_07475"
     CDS             2048..>2620
                     /locus_tag="ACZO9R_07475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0912940.1"
                     /translation="MNPTVSIIVPVYNAKNYLQRCIDSILHQEYTDYELFLVDDGSTD
                     GSGDICDAYAAADARITVIHKENTGVSDSRNLAISQARGTYLQFLDSDDWITPDATKL
                     FVRAATEHDCDLVIADFYRVVGERVSHKGDIEDDGVLTREEFAAHMMENPADFYYGVL
                     WNKLYRRDLVEKYHLRMDVSVNWCEDFLFNL"
BASE COUNT          506 a          804 c          533 g          777 t
ORIGIN      
        1 cgtcgtcacc ttattatctt ctgccgccgt cttaatcccc tccgagacct ctgtctgcgc
       61 gctctgctct gttcctccat cgctcccgcc gcagcctgca agcgccgtta cggcaagacc
      121 gccgctgacc ataagtgcca gccattttct gtaattcctg ttcccacacg ttttcatctc
      181 ttcttctccc gcaaaaaatg tagctgcgca accggttacg cagctacatt ttatcacata
      241 aagacgttta tttcaacctc cgttctgcca gaaccaccgt ttgtcccgac atgctctgtg
      301 tttctctgat cggcgcaaac tgattttttc tccagaaatg ctctgcctgc gggttgccct
      361 tcacccagca gagccgcacg gtgcggattc cttcctctgc cagatgacgg cacagatcct
      421 ccacaattct ggtgccgatc cccgcctgct gaacagaagc ctccatcata aaaagtccga
      481 tatacgccat cgcccgctcc gggtaatcgg caatgagatc catctgtgca agcagtgtct
      541 ctccccggaa aaatcccacg taatacttat catcggggag ttttccgggc ggaagcgcca
      601 caagatctga caggatgctg tctcttgtca catacggggg acaatattca taatacagat
      661 tgttcttccg ggagagttca taaatagctc ccacatcctg ctctgtcagt acgcgtacct
      721 gatatttttc cgaccagcgg ctgatgtcca ttctcttaca gcgtccccgg atctgtccgt
      781 cttttccgac ccagacaagc attccctcat ccagtcccat gttccacacc tgctgctcct
      841 ccatgcgcac caggcaggtt tctccttgtt cgtttaccag cgtgatctca tcatacgccg
      901 cttccccgtc atcacgtcct tcacagccaa aatccggctc gtcaatccta agaatccgat
      961 attgcttctg tccgtccttc attgtcgtat ctctttctct ttgttttcat tctcccggca
     1021 tccggtcagc ctttataatc gatgtgtccg ccgaccaagc gttcctcttt cgtctgcacc
     1081 gtattcatcc gctcattctg ctggtaatcg ctgatctttt ttaacagctc ctcgatactt
     1141 gacgccgtga tcgtcaccgt attttcccag tctgcctcag acgcctcatc tgtcttattc
     1201 acacgctctg ccgcatgttc gtctctttga ttttttccgt ccctgatccg cttctcctgc
     1261 gcgtctttct gcgcccgtct cgcctccgcc ttcttttctt ccgccgcctt ctcgcgcttt
     1321 ttctcaatcc gctccttctg cgcgtcggaa gacttctgca gcacggcaaa ataggtggcg
     1381 gtgccgccat cattcacctg catgccgtag ccttttactt ttgtgccgga agcctccagc
     1441 gaacttttca tctgtgcaat gccgcttccc gcattcgcaa tgatcgcctc gtactgtctg
     1501 cggtaatttt catcctccgc catgcgctca atcttgtcct cgtcgatgag aactaccatc
     1561 ttcgatgcat tggcatagct tcccgcctgc gtccttgcct tctccttctg atcctcgctg
     1621 accagaataa agtccatatt ggaatatttt cttttcagtt cttcataata ctttgccgct
     1681 ttttcgctga gtttcggatt gccgatcgtt cttccagtca ccgttccggt ttttcctgtc
     1741 ctgtctgtct tgtccgcctt attggctttc ccagtcttct ctgacgatgc gtttccatcc
     1801 gtcttttctt ttccttttgt gtctgccgcc ttacggttat aggacatggc acttcccact
     1861 gcatttgcat aatccggtat caaactcata caaatctccc ctttcgccgt ttccgcctgc
     1921 cgaaatccgt ttcggcaggt ctgctctctt tactttatat atcggtcaga tatgtccatc
     1981 ttgccattgc aaatttaaaa aatcaaaggt aagataaaag gagtatcttt tcgggaggtc
     2041 tcttttcatg aaccctacag taagcattat cgtacctgtc tacaatgcaa aaaattatct
     2101 gcagcgctgc attgacagca ttctgcatca ggaatatacc gactacgaac tgttcttagt
     2161 cgacgacgga agcacagacg gctccggtga catctgcgat gcttacgccg ccgcagatgc
     2221 ccgcattaca gtcatccaca aagaaaatac cggcgtatcg gacagccgta atctcgccat
     2281 cagccaggct cgcggcactt acctgcagtt tctcgacagc gatgactgga tcacaccgga
     2341 tgcgacaaag ctgttcgtgc gcgccgccac agaacatgac tgcgatctgg tgatcgccga
     2401 tttctaccgc gtcgtcggag agcgtgtctc gcacaagggc gatattgagg atgacggtgt
     2461 cctcacacgc gaagagtttg ctgcgcatat gatggagaat cccgctgatt tttattatgg
     2521 cgttctctgg aacaagctgt accggcgtga tcttgtggaa aaatatcatc tgcgcatgga
     2581 tgtctcggtg aactggtgtg aggacttttt gtttaatctg
//
LOCUS       DBMDQJ010000336         2615 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13942_length_2615_cov_1.662689, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000336 DBMDQJ010000000
VERSION     DBMDQJ010000336.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13942_length_2615_cov_1.662689"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            133..882
                     /locus_tag="ACZO9R_07480"
     CDS             133..882
                     /locus_tag="ACZO9R_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912941.1"
                     /translation="MCIDTERFERARGRVIGIERQRLGIGTLSEKTVHAVLKNYYAPD
                     EDMHEIPIENFVADIFDGEAVIEIQTRSFQNMRRKLAAFLPLYPVTIVYPIPHVKWLH
                     WMDEETGEISPGRKSPKKGNAYQAFIELYKIRPFLKDPNLRLRLDLIDMEEYRLLNGW
                     SRDKKKGSDRFDRIPLTFVEEVRVDRREDYMQFIPYEIPEEFTAKDFAACAKIPVRLA
                     QTVLLLLTDLLIVERIGKQGRSYLYRICEIE"
     gene            983..2545
                     /locus_tag="ACZO9R_07485"
     CDS             983..2545
                     /locus_tag="ACZO9R_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HIR0912942.1"
                     /translation="MKNTAIKLSGILLAGAVFFSTLLGYGTQVAAATAIGSDTEAGQA
                     SGDGSVTSVADLPGKHIGVQLGTTGDIMVSDYETDGSGTVVERYNKGADAVQALKQGK
                     LDCVIIDELPALAFVEQNEGLKILEENFVEEDYAFVIAKGNEALVDQVNETLRELKDE
                     GVLDSIAKNYTGTDEEIGKYPYEILDVERTNGTLTVGTNAEFPPYEYYEEGKITGIDM
                     DIMQAVCDKLGMELKIEDMAFDSVIPAVSTGKVDIGASGFTVTEERKKNVNFTDTYAK
                     SKQVIIVRDTSVTAEKTGIAEKFYDNFIKDSRYTYLLKGLLNTLIITVFAVMIGIVFG
                     FLIAIVRTNHDRNGGLTILNALCKAYLAVVRGTPVMIQLLIIYYVIFQSVNTGKLFVA
                     VIAFGLNSAAYVAEIVRSGIMAVDIGQFEAGRSLGLNYKQTMLSIILPQAVKNILPAL
                     CNEFISLLKETSISGYIGLMDLTKGGDIIRSVTYEAFMPLIAVAAIYFLIVMGLSKAV
                     GLLERRLRSNER"
BASE COUNT          689 a          532 c          777 g          617 t
ORIGIN      
        1 agctgatccg tcgtgccgag acgccggagg actatttccg cacgtttgac tgctttgata
       61 tcttaaagga tatgaagcac ccgaaataag cggcagatca ttccggaaaa agcacagcag
      121 cgaggatgag atatgtgcat tgatacagaa cgttttgagc gtgccagggg cagagtcatt
      181 ggaatcgagc ggcagaggct tggaatcggc acactctcgg agaagacggt acatgcagtg
      241 ctcaagaatt attatgcacc ggatgaggat atgcatgaga tcccgattga gaattttgtt
      301 gcggatattt ttgacgggga ggcagtgatc gagattcaga cgcggtcgtt ccagaatatg
      361 cggcggaaac ttgccgcatt tctgccgttg tacccggtga ccatcgtcta tccgattccg
      421 catgtgaagt ggcttcactg gatggatgag gagacggggg agatctctcc cgggcgcaaa
      481 tcgcccaaga agggaaatgc ataccaggca ttcatcgaat tgtataagat ccggccgttt
      541 ctgaaagatc cgaatctgag actacggctg gatctcattg acatggagga ataccgtctg
      601 ttaaacggct ggagccggga caaaaagaag ggaagcgacc gcttcgaccg gataccgctt
      661 accttcgtgg aggaagtgcg tgtggaccgg agggaggact acatgcagtt catcccctat
      721 gagattccgg aggagtttac tgcaaaggat tttgcggcat gcgcgaagat tccggtgcgg
      781 ctggcacaga cggtgctcct gctcctgacg gatctattga ttgtggagcg gattggaaag
      841 cagggaagaa gttatctcta ccggatctgc gagattgaat gaatctcttc ccaagcgggg
      901 cgttctgtgt tatactgact gccggatgta tatttatgca gtatctatgt gagagataag
      961 gaagcatgag gtagatagta cgatgaaaaa cacagcaatc aaattatccg gcatactgct
     1021 tgccggagcg gtcttttttt cgacgctgtt aggttacggc acacaggttg ccgcggcgac
     1081 ggcgataggc agcgacacgg aggcgggaca ggcatccggc gacggttctg ttacgtcggt
     1141 tgcggatctt ccgggaaagc atatcggtgt acagctcggc acgaccggag atatcatggt
     1201 cagcgactat gagacggacg gctcgggcac ggtagtagag cgttacaata agggcgcgga
     1261 tgccgtgcag gcattaaagc agggaaagtt agactgcgtg attattgacg aattgccggc
     1321 gctggcattt gtggagcaga acgaagggct taagattctc gaggagaact ttgttgagga
     1381 ggactatgcg ttcgtaatcg caaagggtaa cgaggcactt gtggatcagg tcaatgagac
     1441 gttgcgcgaa ctgaaggacg agggtgttct cgatagcatt gcgaagaatt ataccggaac
     1501 ggacgaggag attggaaagt atccgtatga aattctggat gtggaacgca cgaacggcac
     1561 gcttaccgtt ggtacgaatg cggagtttcc gccgtatgaa tactatgaag agggaaagat
     1621 caccggaatc gacatggata ttatgcaggc ggtctgcgac aaactcggca tggagttaaa
     1681 gatcgaggac atggcgtttg attccgtgat tcctgcggtg tccaccggaa aagtcgacat
     1741 cggtgcatcg ggttttacgg tgacggagga acgcaaaaag aatgtgaatt tcacggatac
     1801 ctatgcaaaa tccaagcagg tcatcatcgt gagagataca tctgtcacag cagagaagac
     1861 agggatagca gagaagtttt atgataactt tatcaaggac agccgatata cctatttgtt
     1921 aaagggactt ttaaatacac tcatcatcac cgtgtttgcg gtaatgatcg gtattgtgtt
     1981 tggatttttg atcgcgattg taagaaccaa ccacgacaga aacggcgggc ttacgatctt
     2041 aaatgcactc tgcaaggcat accttgccgt tgtgcgcggt acgccggtca tgattcagct
     2101 tctgattatt tattatgtca ttttccagag cgtcaatacg ggaaaactgt tcgtggcagt
     2161 cattgcgttc ggactgaact cggcggccta cgtggcggaa atcgtccggt ccggtattat
     2221 ggcggtggac atcggacagt ttgaggcggg aagaagcctt ggattaaatt ataagcagac
     2281 gatgttaagc atcatcctgc cgcaggcagt caaaaatatt ctgccggcac tctgcaatga
     2341 gtttatcagt ctgttgaagg agacttcgat cagcggttac atcgggctga tggatctgac
     2401 caagggcgga gacatcatcc gaagcgtgac gtacgaggcg ttcatgccgt taattgcggt
     2461 cgcggccatt tatttcctga ttgtcatggg actgagcaag gcagtcggac tgttggaaag
     2521 gagattgaga agcaatgagc gataatacgg taattctgga agtaaaagag cttcacaagt
     2581 cttttgacgg tcatgaagtg ttaaaaggaa tctcg
//
LOCUS       DBMDQJ010000337         2612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13964_length_2612_cov_1.682597, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000337 DBMDQJ010000000
VERSION     DBMDQJ010000337.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2612
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13964_length_2612_cov_1.682597"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            11..81
                     /locus_tag="ACZO9R_07490"
     tRNA            11..81
                     /locus_tag="ACZO9R_07490"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Gly,seq:tcc)
     gene            233..880
                     /locus_tag="ACZO9R_07495"
     CDS             233..880
                     /locus_tag="ACZO9R_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR0912943.1"
                     /translation="MEKKADFHSLSRKAKVQYVWDYYKWPIAAAIAALCFVIYLIYHY
                     ATYRDPLLNVIMMNCNDSITADSKGFDEFLEACGYDPKEDSVSLTSSLQFSDGEYSTS
                     YNDTQVLTLMLAAGGQDLFFGTGDEYLDYADQGALMDLSTVLSDELLDRYQDHLIYTT
                     EDGAVASYPCAIELTDNAWLHKYNYYDSCYFGILYQNQNLDAALQFADFLLNYND"
     gene            complement(938..>2612)
                     /locus_tag="ACZO9R_07500"
     CDS             complement(938..>2612)
                     /locus_tag="ACZO9R_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR0912944.1"
                     /translation="KDAATGTAEAAAGQDTETAAGQGTDAQAGAQENLEETALSLMRE
                     VNPAEAAGTDDLYRSWYEVFVYSFYDGDGDGIGDLPGLTEKLDYINDGNPATDTDLGC
                     DGIWLMPVMPATTYHKYDVTDYCAIDEQYGTMEDFETFVNACHERGIRVMIDFVMNHT
                     SSQHPWFQTAAEYLKDLPEGMEPDTEACPYVDYYHFSREKGSGYCQLAGTDWYYEAQF
                     WSEMPDLNLESEAVRQEFDEITDFWLEKGIDGFRLDAAKEYETGSIDSNIEILSWFNN
                     MVKEKKSDAYIVAEVWSDLETYAQYYQSGIDSSFDFTFADKDGVIAKAVKGTSGASSY
                     GKAIVRLQDALGTYSDSYIDAPFYTNHDMGRGAGYYSGDTMEAQTKLAQAMNLLMSGS
                     SFLYYGEELGMKGAGKDENKRAPMYWSKDAGAEGMCLGPQDMDAITMIYDSLAEQQDD
                     GNSIYWYVKEALRLRNAYPEISHGTAEFLENLSDDAVCVLRKNWQDSSVIVVFNCTKD
                     ERTVDLSQISADSESGDLKLAGELLTGTRAASWQDGTLTLPAYGVAILK"
BASE COUNT          550 a          810 c          554 g          698 t
ORIGIN      
        1 ggcacttcat gcgggtgtag ttcaatggta gaacaccagc cttccaagct ggatacgtgg
       61 gttcgattcc catcacccgc ttttcttaat tggagagcga aaaagaacag agcagatgga
      121 gagattctct cactgctctg tttttttata attttttcat tcttttttcc tttttaacct
      181 tccatttcta tgttattctt gtactagttc attttagaaa cggagtacca ttatggagaa
      241 aaaggctgat tttcattcac tttcccgcaa ggcgaaagtg cagtatgtct gggattatta
      301 caagtggccg atcgccgctg cgatcgctgc gctctgcttt gtcatttacc tcatctatca
      361 ttacgcgacc taccgcgacc cgctgttaaa cgtcatcatg atgaactgca acgattccat
      421 aaccgccgac agcaagggct tcgatgaatt tctcgaagcc tgcggctacg acccgaagga
      481 agattccgtc tccctcacat cgtccttaca gttctcggac ggcgagtatt ccacgtccta
      541 caacgacacg caggtgctga cgctgatgct ggcagccggc gggcaggatc tctttttcgg
      601 aaccggcgat gaatatctcg attacgccga ccagggagct cttatggatc tgtccaccgt
      661 attatccgac gaactgctgg accgctacca ggatcatctc atctacacga ccgaggacgg
      721 tgccgtcgct tcctatccct gtgcgatcga gctcaccgac aatgcctggc ttcacaagta
      781 caactattat gattcctgct atttcggcat tctctatcag aatcagaact tagacgccgc
      841 cttacagttt gcggactttc ttctgaatta taacgactga tcgttttctc tcctgatgct
      901 gccggcagca gagcggctga tttgtttttt cagatctcta cttcaggatg gcaacaccgt
      961 acgccggaag cgtcagcgtt ccatcctgcc aggaagccgc acgggttccg gtcagcagtt
     1021 ctcctgccag cttcaaatcc ccgctttcac tgtctgcgga tatctgtgac agatccacgg
     1081 tgcgctcgtc cttcgtgcag ttaaacacga cgatcaccga ggaatcctgc cagttcttgc
     1141 gcagcacgca cactgcgtca tccgacagat tttcaagaaa ctctgccgtt ccatgcgaga
     1201 tctccggata ggcattccgc agacgcagcg cctccttcac ataccagtag atggaattgc
     1261 cgtcgtcctg ctgctccgca agactgtcat agatcatcgt gatcgcatcc atatcctgcg
     1321 gtccgaggca catcccctct gcaccggcat ccttgctcca gtacatcggc gcgcgcttgt
     1381 tctcatcttt tccggcgcct ttcattccca gttcttctcc ataatataag aagctgcttc
     1441 cggacatcag aagattcatc gcctgcgcca gtttggtctg cgcctccata gtatcgccgg
     1501 agtaataccc tgcgcccctt cccatatcat ggttggtgta aaacggcgca tcgatatagc
     1561 tgtcactgta agttcccagc gcgtcctgca gccgcacgat cgcttttccg taagaagagg
     1621 caccgctcgt tcccttgacc gccttcgcaa tcacaccgtc cttgtctgca aacgtaaagt
     1681 caaagctgct gtcaattccg ctctggtaat actgtgcgta tgtctccaga tccgaccaca
     1741 cctctgccac gatatacgca tcgctcttct tttcttttac catattatta aaccaggata
     1801 gaatttctat gttcgagtcg atgcttccgg tctcatactc ttttgccgca tccagacgga
     1861 aaccatctat gcccttttcc agccagaaat ctgtaatctc atcaaactcc tgccgcaccg
     1921 cttcactctc cagattcaga tccggcatct cgctccagaa ctgtgcctca taataccagt
     1981 ccgtccctgc cagctggcag tatccgctgc ctttctccct ggaaaaatga taataatcca
     2041 catacgggca tgcctctgta tccggctcca ttccctccgg cagatccttc agatactctg
     2101 ccgccgtctg aaaccacgga tgctgcgacg aagtatgatt catcacaaaa tcaatcatga
     2161 cgcggattcc ccgctcatgg catgcgttca cgaatgtctc gaaatcctcc attgtgccat
     2221 actgctcatc gatcgcacag tagtccgtca catcatattt gtggtaagtc gtcgccggca
     2281 tcaccggcat cagccagatc ccatcacatc ccagatctgt atccgttgcc ggattaccgt
     2341 cattgatata gtccagcttc tccgtcagtc ccggaagatc tccgatgcca tccccatcgc
     2401 cgtcatagaa gctgtaaacg aacacctcgt accagctccg gtagagatca tccgttcctg
     2461 cagcttctgc cgggtttacc tcccgcatca gagacagcgc cgtctcttcc aggttctcct
     2521 gtgctcccgc ctgcgcatcc gtgccctgcc cggctgccgt ctccgtgtcc tgcccggctg
     2581 ccgcttccgc cgtacctgtt gccgcgtctt tc
//
LOCUS       DBMDQJ010000338         2611 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13970_length_2611_cov_1.668924, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000338 DBMDQJ010000000
VERSION     DBMDQJ010000338.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13970_length_2611_cov_1.668924"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="ACZO9R_07505"
     CDS             complement(<1..970)
                     /locus_tag="ACZO9R_07505"
                     /inference="COORDINATES: protein motif:HMM:NF013697.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR0912945.1"
                     /translation="MAKQCRIALSLLLVLLTLVSLTACAGNQPTADGTSSQTGTSDTS
                     KDSVANADGRTKITYLLDQGLVAGYQPVVDAYNESQVKYYVEFLILPQDTTTVHDDLV
                     NKLAVGDTSVDVFNLDGTYVTEFAAAGWLTDFGEYFSGSEIENLLPSTREQMSYNGEL
                     VAMPGLTNGSMLFYRKDVLEEIGAEVPTTYEGWMELADQAVGVNGVEYLAVFQAAQGE
                     SLVCNWCEFIWNNGADLFDADGNPQANSPKIVEATKIMADLAQNYAPSGITTYAETES
                     EQVFLEGKALILRDWVHFWKTSNDEEKSKVAGKVGMTLLPIGPEGDT"
     gene            complement(1098..2111)
                     /locus_tag="ACZO9R_07510"
     CDS             complement(1098..2111)
                     /locus_tag="ACZO9R_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0912946.1"
                     /translation="MTIRDIAKKANVSVATVSRVINQSKSVSPEIEQRVRAIIKESGF
                     QPNSMAKGLVTKQSKMIGLIIPDISNTVFGALTKGINSVCQKEGYTLMVCESGGTKEK
                     ELALLEVLSSKSIDGLLFAGVDVDDSLVSLMKAKDFPVLLVTQEASGQTGQLPTVAHD
                     NIQAVYEAVTFLINNGHRRIAFIGGRKDDYSSGKKRLIGYQKALEENAIQVPDAYIEY
                     GEFSFESGNRCMKKIYEENEVLPTAVMACSDLIAFGAIQFLRTVQIPVPDEISVMGFD
                     DLELTQYFAPALSTVRISYFDEGAQAAKTLFEQMNAEKVSLEPIYLPHKIIRRSSTKR
                     IIP"
     gene            complement(2136..>2611)
                     /locus_tag="ACZO9R_07515"
     CDS             complement(2136..>2611)
                     /locus_tag="ACZO9R_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912947.1"
                     /translation="NGRTMGEDLELPGGGAEVKIDWDLASVTLPVTKLELIVNGEIRE
                     MLDLDSFLGEKSGGFICKITEPSWIALRVRGQYENTPELIAAHTSPVSCLIDGVRRFS
                     AENAQTVLDLIECTEKYIRDLAPKAEEPKYQQVMAYMEQARQKVLSQMKEAAAGK"
BASE COUNT          607 a          743 c          553 g          708 t
ORIGIN      
        1 gtgtatcacc ctcagggccg atcgggagca gcgtcatgcc aactttccca gcgaccttgg
       61 acttctcttc atcattggaa gttttccaga aatgtaccca gtcacgcaga atgagcgcct
      121 ttccttccag gaagacctgc tcgctctccg tttcagcata agtcgtaatg ccggagggcg
      181 cataattctg agcaagatct gccatgatct ttgtagcttc tacgatcttt ggggagtttg
      241 cctgcgggtt gccatctgca tcaaacagat ctgcaccgtt gttccagatg aactcgcacc
      301 agttgcagac cagagactcg ccctgtgccg cctggaacac tgccagatat tccacaccgt
      361 tgacaccaac tgcctggtcc gcaagttcca tccagccctc ataggtagtc ggaacttctg
      421 caccgatctc ttccagtacg tctttcctgt aaaaaagcat actgccattg gtcaggccgg
      481 gcatcgcaac gagctcgcca ttgtacgaca tctgttcacg tgtagacggc agcagatttt
      541 caatctcaga tcccgagaaa tattcgccga aatccgtaag ccatcccgcc gccgcaaact
      601 ctgtgacata tgtaccatca agattgaata catctacact ggtatctccg actgccagct
      661 tgtttacgag atcatcatgc acggtcgttg tatcctgcgg aaggatcaaa aactctacat
      721 agtacttgac ctggctttca ttataggcat caaccaccgg ctgatatccc gcgaccaacc
      781 cttgatcaag caaataagtg atcttagtac gcccatctgc attcgcgaca gagtcctttg
      841 aagtgtctga tgttccagtc tgggatgatg tgccgtccgc cgtgggctgg ttccctgcac
      901 acgcagtcag agaaaccagg gtcaaaagca ccagaagcaa gctgagcgca attcggcatt
      961 gttttgccat attcttttcc tcctaatatt ttagtttttt ctatgttgac ctcccgtacg
     1021 cgaaggttaa cattttgcag gaaaacgttt tcctatctgt tcaaatatat gccctgtcga
     1081 aacagggcgt atatatttta gggaatgatc cgttttgtac tacttcgacg gatgatcttg
     1141 tgcggcaaat aaatcggctc cagagacact ttttccgcgt tcatctgttc gaacaacgtc
     1201 tttgcggcct gcgcgccctc gtcaaagtat gagatgcgga ccgtagataa cgccggcgca
     1261 aaatactgtg tcaactccag gtcgtcaaat cccattacgg aaatctcatc cggaactgga
     1321 atttgcactg tgcgtaagaa ttgaatggct ccaaacgcta tcaaatcact acaggccatc
     1381 accgcagtcg gcaatacctc gttttcttcg tagatcttct tcatacacct gttgcccgac
     1441 tcaaaggaaa attctccata ttcgatgtaa gcgtccggca cctgaattgc attttcctca
     1501 agcgcctttt gatacccgat taagcgcttt ttaccggatg aataatcatc ctttctgccg
     1561 ccaataaatg caatcctccg atgtccgtta ttgatcagaa acgtcactgc ctcgtaaacc
     1621 gcctgtatat tatcgtgtgc tacagtcgga agctgtccgg tctgaccgga cgcttcctgt
     1681 gttaccagca gaacaggaaa atcttttgct ttcatcaggg acacaagact atcatcaacg
     1741 tctacgccgg caaacagcag accgtctata cttttactac ttaatacctc cagcagcgcc
     1801 agttcttttt ccttcgtacc gcccgactca cagaccatca gcgtgtatcc ttctttttgg
     1861 cagacgctgt tgattccctt ggtcagcgcg ccaaacactg tattggagat atccgggata
     1921 atcaggccaa tcattttact ctgcttcgtc actaggccct tggccatgga gttcggctga
     1981 aaaccactct ccttaatgat cgcccggacc ctctgttcga tctctggact gactgactta
     2041 gactgattga tcactctaga tactgttgcc acggatacat tggccttttt tgctatgtct
     2101 cgaatcgtca tttagcgtct cctttgaaag gatttttatt taccagctgc cgcttccttc
     2161 atctgcgaca ggaccttttg ccgtgcctgt tccatgtacg ccatgacctg ctgatacttt
     2221 ggttcttccg ctttcggtgc aaggtcacgg atatatttct ctgtgcattc gatcaggtcc
     2281 agaacagtct gcgcattctc cgcgctgaat ctccgcacac cgtcaatcag gcacgaaacc
     2341 gggctcgtat gcgctgcaat cagttcagga gtgttctcat actggccgcg cacacgcagc
     2401 gcgatccacg aaggctcagt gatcttgcag ataaaaccac cggacttctc tcccagaaat
     2461 gagtcaagat ccagcatttc acggatctct ccgtttacga tcagctccag cttggtcaca
     2521 ggaagcgtca cactcgccag atcccaatcg atcttcactt ccgcgccgcc tccaggcaat
     2581 tccaagtcct cgcccattgt gcgcccattg g
//
LOCUS       DBMDQJ010000339         2607 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_13995_length_2607_cov_0.984038, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000339 DBMDQJ010000000
VERSION     DBMDQJ010000339.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13995_length_2607_cov_0.984038"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..684)
                     /locus_tag="ACZO9R_07520"
     CDS             complement(<1..684)
                     /locus_tag="ACZO9R_07520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912948.1"
                     /translation="MDDRYVAKRVIYLLGMSAATILFTTAAAFIRGQYLDEWIVLVII
                     NLVFLALWFFLLEHCRMKRSIAGNRETSYRRIFAGYLASLGVTAVCLVLPEFSRPVML
                     SAILMLGVGNIELSLCSGLFFCSLLGLLCGGTIQEFSMYILLVLIGAVAADAVENSKK
                     QFWYEWFAGMAGMLLPVLFYYLTYREISVRIMVSDVADGVVVFLFLHLCYRKLADARN
                     AEVDEVMVDI"
     gene            complement(677..1723)
                     /locus_tag="ACZO9R_07525"
     CDS             complement(677..1723)
                     /locus_tag="ACZO9R_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR0912949.1"
                     /translation="MSLTEVSLNIPTDHMANVFGQFDVYIKKIERTLNVTVVVRGEDM
                     KILGDERRCRRAQDVFMQLLELSKRGNVITEQNVNYALALMAEEKESAIVEIDRDCIC
                     HTINGKPVKPKTLGQKAYVDAIREKMIVFGMGPAGTGKTYLAMAMAITAFKNEEVGRI
                     ILTRPAIEAGEKLGFLPGDLQSKVDPYLRPLYDALYQIMGAESFQKNMEKGLIEVAPL
                     AYMRGRTLDNAFIILDEAQNTTPAQMKMFLTRIGFGSKAVITGDATQKDLAPDAASGL
                     DIALKVLRNVEEIGICELTSQDVVRHPVVQKIVKAYEDYEKRAGKTSGTRKNTSRTGA
                     GRTGARSRRENTNG"
     gene            complement(1710..>2607)
                     /locus_tag="ACZO9R_07530"
     CDS             complement(1710..>2607)
                     /locus_tag="ACZO9R_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HIR0912950.1"
                     /translation="SYLTTETILDFLEEEHASFGTKKSRISCEDLEEAMRSRYRDVIW
                     TSIKIYGTKMTVDIQENLLPQEEYEAKSDKPQDIVAAKDGVITEMVTRAGTPLAAVGT
                     EVKKGDILVSGRIEVLNDDGEIMQYLYRPSDADIKAKVLYSYRDVIDGTWVEMAPTGN
                     TKECYSITLLDNVFDWPFFRNPFAEYQVQTESRQLHVADNFYLPVWLNKSEFTEVEQV
                     RHERTEEEIRQIATKNLSDYIKDLEEKGIQIISKNVIIKSTGEKYVVSGDLWAHESIV
                     SYQPTEVYDTTMQERQNENESD"
BASE COUNT          590 a          786 c          570 g          661 t
ORIGIN      
        1 gatgtcgacc atgacctcat cgacttccgc atttcttgca tccgccagct tccggtagca
       61 cagatgcaaa aacaaaaaga cgaccacacc atccgccacg tcagatacca tgatccggac
      121 gctgatctcc cggtaggtca gatagtagaa cagaaccgga agaagcatgc ctgccatgcc
      181 ggcaaaccat tcataccaga actgcttttt gctgttttcc actgcgtccg ctgccaccgc
      241 tccgatcaga accagcagga tatacatgga gaattcctgg atggttccac cacacaaaag
      301 accgagcagg ctgcagaaaa agagtccgct gcacagggaa agctcgatat tccccacacc
      361 gagcatcaaa atggcagata gcatcacagg tcttgaaaat tccggaagta caagacagac
      421 cgccgtcacg ccaagcgacg ccagataccc tgcaaaaata cgccggtagc ttgtctcacg
      481 gtttcccgca atgctccgct tcatccggca gtgctccaat aaaaagaacc acaacgccag
      541 aaataccaga ttgatgataa caaggacgat ccactcgtcg agatactgtc ccctgataaa
      601 tgccgccgct gtcgtaaaca ggatcgttgc ggcagacatg ccaagcagat atattactct
      661 ctttgccaca tagcgatcat ccattcgtgt tctctctcct gcttctcgca ccggttctgc
      721 ccgcaccggt tctgctcgtg ttctttctcg tgccggacgt cttaccggcg cgcttctcat
      781 aatcctcgta tgccttgaca atcttctgca ccaccggatg ccgcacgaca tcctggcttg
      841 tcagttcgca gataccgatc tcctccacat tgcgcagcac tttcagcgca atgtccaagc
      901 ccgatgcggc atccggcgcc agatccttct gggtggcgtc accggtgatg accgcctttg
      961 aaccgaaacc gattctggtc agaaacatct tcatctgcgc cggcgtcgtg ttctgcgcct
     1021 cgtccagaat gataaacgcg ttatctaacg tacgtccgcg catgtaggca agcggagcca
     1081 cctcaatcag tcccttttcc atattcttct ggaagctttc cgcccccata atctgataca
     1141 gcgcgtcgta aagcggtctt aagtaaggat caaccttgct ctgcagatcg cccggaagga
     1201 agccaagctt ctcccccgcc tcaatcgcag gcctggtaag gataatccgc ccgacctcct
     1261 cgtttttaaa cgcggtgatt gccatcgcca tcgccagata agtcttaccg gtacccgccg
     1321 gtcccatgcc gaacacgatc atcttttcgc ggatggcatc cacataggct ttctgaccga
     1381 gtgtcttggg ctttaccggc ttgccgttga tcgtgtggca gatgcagtcg cggtcgatct
     1441 ccacgatcgc cgactctttt tcctccgcca tcaatgccag tgcatagttg acattctgtt
     1501 ccgtgatgac atttccgcgt ttggacaatt ccagaagctg catgaaaaca tcctgtgcgc
     1561 gcctgcagcg tctttcgtct ccgaggattt tcatatcttc tccccggaca accaccgtca
     1621 catttaaggt acgctcgatt tttttgatat agacatcaaa ctgaccgaac acattcgcca
     1681 tgtggtctgt cggtatattt aacgaaactt cagtcagact cattttcatt ctgcctttct
     1741 tgcattgtgg tatcgtaaac ctctgtcggc tgataggaaa caatggattc gtgcgcccac
     1801 agatcgccgc ttaccacata tttttcaccc gtgcttttta taataacatt ttttgatata
     1861 atttgaatac ctttttcttc caaatctttg atgtaatccg aaagattctt tgtggcaatc
     1921 tgacggatct cctcttcggt gcgctcgtgc cgcacctgct cgacctctgt gaactcactt
     1981 ttattcagcc acacgggcag ataaaaattg tccgccacat ggagctgcct gctctccgtc
     2041 tgcacctggt attctgcaaa cggattccgg aaaaacggcc agtcgaacac gttatcaagc
     2101 agcgtgatgc tgtagcactc cttcgtgttc ccggtcggcg ccatctccac ccaggtgcca
     2161 tcaatcacat cccggtagga atagagtacc ttcgccttga tgtccgcatc ggatggacgg
     2221 tagaggtact gcatgatctc accgtcgtcg tttaacactt caatgcgccc cgagactaag
     2281 atgtcgcctt tctttacctc agttcccacc gcggcaagcg gcgtgcccgc gcgtgtcacc
     2341 atctcggtaa tcacgccgtc tttggcggcg acaatgtcct gcggcttgtc gctcttcgcc
     2401 tcatattctt cctgcggcag gaggttttcc tgaatatcga ccgtcatctt tgtaccataa
     2461 atcttgatgg aagtccagat gacatcccgg tagcggctcc tcatggcttc ttccagatcc
     2521 tcgcacgaaa tccgggattt tttcgttcca aacgatgcgt gttcctcctc cagaaaatcc
     2581 agaatcgtct ccgtggtcag ataggaa
//
LOCUS       DBMDQJ010000340         2593 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14075_length_2593_cov_1.276886, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000340 DBMDQJ010000000
VERSION     DBMDQJ010000340.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14075_length_2593_cov_1.276886"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            379..1743
                     /locus_tag="ACZO9R_07535"
     CDS             379..1743
                     /locus_tag="ACZO9R_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0912951.1"
                     /translation="MVVKSSVLKELEECYREPGNQLVVLYGRTDSEKERLLSAFVQNK
                     KHFYYRARQVAEREQRAQMGRELTARFEVMLQKYTYEEYFNRVKSGDPTKLVVVIDEF
                     QYIAKKDPTFMEAVLKLKAHRLYPGPVMILLCSSAVSWAERELPECIGEAACRKIDRK
                     IKFTNLNFLEVVRALPEYPVAECIKTYGIIGGVPGYINRWNAKADVKTNICRLILSPG
                     GYLYHAAEDLIGESLRELSVYETILAQIAAGQDKLNDLYRTTGFSRAKISVYMKNLAA
                     FDIIQKVVSFETGGWENTKKGVYRIRDNYVNFWFHFIYPHLSDLYMTRPEEFYDRYIA
                     PGLDAYLSRYFVEVCMEYLGLLGMVGKLPLKIARMGTWVGKEGNIDIIAQNEIRQNMV
                     GLCNWQEPELTMEMCEALFLNMKRAKIGAEYYFLFSAQGFAPEVTAMAERDKRFVLVD
                     MNEL"
     gene            1808..>2593
                     /locus_tag="ACZO9R_07540"
     CDS             1808..>2593
                     /locus_tag="ACZO9R_07540"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HIR0912952.1"
                     /translation="MAKSLIITEKPSVAQEFARILGVSGRNDGYIENDGYVITWCVGH
                     LVEMVYPEEYDERYKKWRLEDLPFLPRDYKYNVIPSVSRQYDVVHKMLHREDIDTVYW
                     AGDAGKEGQTIEENIRKFGGVREGMKELRVWIDSQTEEEILRGIREAKDMSEYDNLAK
                     SGVMRTIEDYAMGINFSRAMSVKYGKLLNDAAGTSSYTAIAVGRVMTCVLGMVVIRER
                     EIRNFTETPFYRVVGTFHPEGEEECRITAEWKAIEGSKYFESPL"
BASE COUNT          677 a          528 c          801 g          587 t
ORIGIN      
        1 ccgcaggcgg tgcagtgctc tgcgcagagc ttttgaaagc acagggatac atcacagcga
       61 aataaagaat aaaaaagaat cctgcttgca ggattctttt ttatttcata ggaaatagcg
      121 tgatacgtta atgtgtgaaa gtagaacttc cctatgaaaa taaaaaacgc tccgcgagga
      181 tgcgcagctc gcggtaagga aaatatcgct ttgcgaaccg cttgcgcgcg gagaatttcg
      241 caagcgagat tcttttttat tgcccgaaaa attaaaaaat gatataattt ccgcagaaca
      301 tatggacaga tagaatgtgc cggatatggg atgcggtgtg gcttttcgtg cccggcagga
      361 aagtgcagag gagaagagat ggtcgtaaaa tcgtcggtgt taaaggaact ggaagagtgc
      421 taccgggaac cgggcaatca gctggttgtg ctgtacgggc ggacggactc ggagaaggag
      481 aggctgctta gcgcatttgt gcagaacaag aagcattttt attaccgcgc gagacaggtg
      541 gcggagcgag agcagcgcgc gcagatgggg cgtgaactga cggcgcggtt tgaggtgatg
      601 ctgcagaagt atacctatga ggaatatttt aaccgggtaa agagcggtga tcccacgaag
      661 ctggtagtcg tgatcgatga atttcagtat attgccaaaa aggatcctac ttttatggag
      721 gcggtgctga aactgaaagc gcacaggctt tatccgggac ctgtgatgat tctgctctgc
      781 agctcggcgg tgtcatgggc agagcgggaa cttccagagt gtatcggtga ggcggcatgc
      841 cgcaaaatag accggaaaat caaatttacg aatctgaatt ttctggaagt ggtgcgggcg
      901 ctgcccgagt atccggtggc ggaatgtatc aagacttacg gcatcatcgg tggtgtgccg
      961 ggatacatca accgctggaa cgcaaaagcg gatgtaaaga ccaatatctg tagactgatt
     1021 ctttcgccgg gcggctatct gtaccatgcg gcggaagatc tgatcgggga gagtctgcgg
     1081 gaactgtctg tctatgagac catcctggcg cagatcgcgg ccgggcagga caagctgaat
     1141 gatctgtacc ggacgaccgg attctcacgc gccaagatca gcgtctatat gaagaatctg
     1201 gcggcatttg atatcatcca gaaggtggta tcgtttgaga cgggcggctg ggagaacacg
     1261 aagaaaggtg tgtaccggat ccgcgacaat tatgtgaatt tctggtttca ctttatctat
     1321 ccgcatctgt cggatctgta tatgacccgc ccggaagaat tctacgacag atatatcgcg
     1381 cccggactgg atgcgtacct ctcccggtat tttgtggagg tatgcatgga atatctggga
     1441 cttctcggta tggtgggaaa gcttccgctt aagattgcac gcatgggaac ctgggtcggc
     1501 aaagagggga atatcgacat cattgcgcag aatgagattc gacagaatat ggtcggactc
     1561 tgcaactggc aggaacccga gcttacgatg gaaatgtgcg aggcgctgtt ccttaacatg
     1621 aagcgggcaa aaatcggcgc ggaatattat ttcctctttt ccgcacaggg atttgcaccg
     1681 gaggttaccg ccatggcgga gcgggataag cggttcgtgc tcgtggatat gaatgaactg
     1741 taagttgccc gatgcctgga aaaatgctat aatttgcttc atgcgaaagt aattggagga
     1801 acagattgtg gcaaaatctt tgatcatcac ggagaaaccg agtgttgcgc aggagttcgc
     1861 gcggattctc ggcgtatccg gaagaaatga cggttatata gagaacgacg ggtatgtcat
     1921 cacctggtgc gtggggcatc tggtggagat ggtgtacccg gaggagtatg atgagcggta
     1981 taaaaaatgg cggctggaag atctgccgtt tctgccgcgg gattacaagt ataatgtgat
     2041 cccatctgtg agcaggcagt atgatgtggt acataaaatg ctgcaccgcg aggatattga
     2101 cacggtctac tgggcaggag atgccggaaa agaaggacag acgatcgagg aaaatatccg
     2161 gaagttcggc ggcgtgcgcg agggcatgaa ggagctccgc gtctggatcg attcgcagac
     2221 cgaggaggag attctgcgcg gcatccgtga ggcaaaagac atgtcggagt atgacaatct
     2281 cgcaaagtcg ggcgtgatgc ggacgatcga ggattatgcg atgggaatca atttttccag
     2341 agccatgtcg gtaaagtacg gaaagcttct gaatgatgcg gcgggaacat cgagctatac
     2401 ggcgatcgcg gtcggccgcg tcatgacctg cgttcttggc atggttgtga tccgggagcg
     2461 cgagatccgc aattttacag agacgccatt ttaccgtgtc gtgggaacct ttcatcccga
     2521 aggagaggaa gaatgccgga tcacggcgga gtggaaggca atagaaggct ctaaatattt
     2581 tgagtcgccg ctt
//
LOCUS       DBMDQJ010000341         2586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14113_length_2586_cov_1.745674, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000341 DBMDQJ010000000
VERSION     DBMDQJ010000341.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14113_length_2586_cov_1.745674"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="ACZO9R_07545"
     CDS             complement(<1..439)
                     /locus_tag="ACZO9R_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912953.1"
                     /translation="MSKDEYLITDAPLKALTVFAMPMILGSFFQQVYNMADSIIVGQY
                     VGSSALAAVGACAALTNVFICVALGAGVGAGVLVSRYFGARDYGKMKTIVSTSLISFL
                     ILSVLLGVFGFHFSHPMMSLLQTPTDILEEAVLYLRVYFVGFPF"
     gene            complement(553..2223)
                     /locus_tag="ACZO9R_07550"
     CDS             complement(553..2223)
                     /locus_tag="ACZO9R_07550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0912954.1"
                     /translation="MTEEKYTIHKYKRILFYVVILVLFAAFFLGQSIYPSERETVTEE
                     SITYTGTFYRELPDGTEEEIRVPDQYDVPAGELMVIRSVLPQDYNENTIAIRSSLENV
                     RIYIGGELRTVYDPKNTRPFGKNSASGYVFCETSGEDAGQEVRIELQSFTEKYSGVVN
                     TVYCGDKLDIWGYIFHCYFMVTLIACTMLFSGLVVLIISLVLDIVYKTRFDLEYLGWC
                     MILGAVWMLGESKLRQLFVPNASILSNMCFFVVMICPVPIMFYIDSVQQGRYRKVYHV
                     AECIVCVNFVICTVLQVLNIADFISTMFLSHIVIAGTFLTIFITICRDLIKGTAKHYK
                     LPLIGLVAAMIAVMLEMTAVYRVVSLSGIFIAIGLVALLVVTLIQTMDRIRELELARQ
                     KEARETLDYLTGLPMRHKGETLIIETMQEHDGCLGFVDMDNLKKINDVYGHKAGDYAL
                     RLVGAMLTECMENAVVCRLGGDEFLFYLPEVSEVEMNTQMRKLFDSFEAAKNATTETL
                     PASLSCGLCMCRQGESFEEYYIRADKALYYVKQNGKNNYRFYEELVEQ"
     gene            complement(2387..>2586)
                     /locus_tag="ACZO9R_07555"
     CDS             complement(2387..>2586)
                     /locus_tag="ACZO9R_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR0912955.1"
                     /translation="QRGSEKFYIQVSDNISGQETFERECSSLLQIRDAYPKMIIARTR
                     HPKYSYEGVEIHDIADWLLQE"
BASE COUNT          714 a          688 c          498 g          686 t
ORIGIN      
        1 aaaacggaaa tcccacaaaa tatacccgca gatacagtac agcttcctcc agtatgtcag
       61 tgggtgtttg taaaaggctc atcatcggat gcgaaaaatg aaagccgaat acaccaagaa
      121 gcacgcttag gatcagaaag ctgatcaaag atgtcgacac aatcgttttc attttgccgt
      181 aatctctggc accaaaatag cggctcacca gtacgcctgc accgacaccg gcaccaagtg
      241 ccacacaaat aaatacattt gtgagtgccg cgcatgcacc gacagccgca agtgcagagg
      301 aaccaacata ctggccgaca ataatggaat ccgccatatt ataaacctgt tgaaaaaagc
      361 tgcccagaat cataggcatt gcaaatacag tcaatgcttt gagtggggca tctgtgatca
      421 ggtattcatc ttttgacatt attttatttc tcctaaattc ctgcttttat ctgggatttc
      481 tgccacgatt tattccagtg tcaatacatc gaccggcatc aggtattcta aaatggcatg
      541 gaatatcgct gcttactgtt ccaccagctc ctcataaaac cggtaattgt tcttgccgtt
      601 ctgtttgaca taatacaatg ccttgtccgc tcttatatag tattcttcaa agctttctcc
      661 ctgtctgcac atgcacagac cgcaggataa ggatgccggt aaagtctccg tcgtggcatt
      721 ttttgccgct tcaaagctgt caaacagctt tctcatctgc gtgttcatct caacttctga
      781 tacctcaggc agatagaaca gaaactcatc tcctcccagc cggcagacca ccgcattctc
      841 catgcactct gtgagcattg ctcctaccag cctgagcgca taatcccccg ccttatgtcc
      901 atagacatca ttgatcttct tgagattatc catatccaca aatcccagac agccatcatg
      961 ctcctgcatt gtttcaataa tcagtgtctc acctttatga cgcatgggaa gtcctgtcag
     1021 gtaatccaag gtttccctgg cctctttctg tcgtgccagc tccagctcgc ggatgcggtc
     1081 catcgtctgg atcagggtaa cgaccagcag agccaccagc ccgattgcga taaagattcc
     1141 ggacagagac acaacacgat acaccgctgt catctccagc atgactgcga tcatggcagc
     1201 taccagtccg atcagcggaa gcttgtaatg tttcgctgtt cccttgataa gatcccggca
     1261 gatggtgata aatatcgtca gaaacgttcc tgcgatcact atgtgggaca agaacattgt
     1321 cgaaataaaa tctgcaatat tcagtacctg taagaccgtg catatcacaa aattcacaca
     1381 aacgatacac tctgccacat gatagacctt ccggtatctt ccctgctgca cactatcaat
     1441 atagaacatg attgggaccg ggcagatcat cacaacaaag aagcacatat tcgacagaat
     1501 agaagcattg ggaacaaaca gctgacgcag cttcgattct cccagcatcc acactgctcc
     1561 caggatcatg caccatccca gatattccag atcaaatctt gtcttgtata caatatcgag
     1621 caccagactg atgatcagca cgaccagtcc ggaaaacagc atcgtgcagg cgatcagagt
     1681 taccataaaa tagcagtgaa atatatagcc ccagatatct aatttatccc cgcaatatac
     1741 cgtgttgacg acaccggaat acttttcagt aaaagactgc agttcaatcc gcacttcctg
     1801 ccccgcatcc tcaccggacg tctcgcagaa cacatacccg ctggcagaat ttttcccgaa
     1861 gggtctggta ttcttcggat catacaccgt cctcagttcc cctccgatat aaatgcgcac
     1921 attctccaga gaggatctga tggcgatcgt attttcatta tagtcctgtg ggagcactga
     1981 ccggatgacc ataagctcac cggcaggcac atcatactgg tccggaaccc ggatctcctc
     2041 ttctgttccg tctggcagct cccggtaaaa ggttccggta taagtaatgc tttcctccgt
     2101 gaccgtttcc ctctcactgg ggtaaatgct ctgccctaaa aaaaatgctg caaacagcac
     2161 aagtatcacc acataaaata aaattctttt atacttatgg atcgtatatt tctcttctgt
     2221 catttttatt acctatatta aataattccg gggcatttcc cctgctttta tgacgatatt
     2281 gggggtgcag tccaaaatct ttcccccgct catgaacaag tttatcacag gctgcagaaa
     2341 aagtaactat ctattttact ctgtagacgg agatatttca ccctgcttat tcttgtagca
     2401 gccaatcggc tatatcgtga atttcgactc cttcatagct atatttcgga tgtctggttc
     2461 tggcaataat cattttcgga taagcatccc gaatctgaag cagagaagag cattctctct
     2521 caaatgtttc ctgcccggaa atgttgtcgc tgacctgaat ataaaacttc tcgctgcctc
     2581 tctgag
//
LOCUS       DBMDQJ010000342         2586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14114_length_2586_cov_1.671227, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000342 DBMDQJ010000000
VERSION     DBMDQJ010000342.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14114_length_2586_cov_1.671227"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /gene="ftsE"
                     /locus_tag="ACZO9R_07560"
     CDS             complement(<1..750)
                     /gene="ftsE"
                     /locus_tag="ACZO9R_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR0912956.1"
                     /translation="MIKLEHVSKSYSAGIPALNDVSLNIEEGEFVFVVGDSGSGKSTL
                     IKLLLKELEPTEGTITINGRKLNKIRRRQIPKFRRNIGVVFQDFRLLKDRNIYDNVAF
                     AQKVIGESNRSIKKNVPKLLSMVGLAAKYRSYPRQLSGGEQQRVAIARALINKPKILL
                     ADEPTGNLDANNAWEIMKLMEEINEQGTTVVVVTHNLEIVKAMNKRVITMQKGVVVDD
                     TASDHYGKDTSVEQDDDLFDDFLDDGGDDDYA"
     gene            complement(872..1960)
                     /locus_tag="ACZO9R_07565"
     CDS             complement(872..1960)
                     /locus_tag="ACZO9R_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR0912957.1"
                     /translation="MISNQILQNTIDGLKGITRIDLCIIDVEGKVLAATFPEADQYIE
                     PAQAFVESPADSQVVNGYQFFKVFDEHQLEYILLASGDSDDVYMVGKIASFQIQNLLV
                     AYKERFDKDNFIKNLLLDNLLLVDIYNRAKKLHIDTEVRRVVFIVETNRDKDGNELEK
                     IRGIFGTKTKDFVTAVDEKNIIVVKEVGENEGYEELNKIAESMVNLFRADADSDVHVA
                     YGTIVGEIKEVSRSYKEARMALDVGKIFFEEKDVIAYSTLGIGRLIYQLPIPLCKMFI
                     KEIFDNKSPDDFDEETLTTINKFFENSLNVSETSRQLYIHRNTLVYRLDKLQKSTGLD
                     LRVFEDAITFKIALMVVKYMKYMESLDY"
     gene            complement(2152..>2586)
                     /locus_tag="ACZO9R_07570"
     CDS             complement(2152..>2586)
                     /locus_tag="ACZO9R_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR0912958.1"
                     /translation="EQNDFSYEFYRRIERNKRSIFVLGQTEEGVQKVKAELQEAFPKL
                     ILAGEYALENCVGNLDAVINEMNATTPDVIVSILPSPLQEHFFAEHKDKMSATIWFGM
                     GELGVHGKKHGLGSFVKNRLHLGRLKNSMNKYQDQEKFGEAE"
BASE COUNT          618 a          732 c          487 g          749 t
ORIGIN      
        1 cgcatagtcg tcgtcacctc catcatccaa gaaatcatca aacagatcat cgtcctgttc
       61 gacagaggta tccttaccgt aatgatccga agcggtatcg tctaccacaa cgcctttttg
      121 catcgtgatg acacgcttat tcatcgcttt cacaatctcc agattgtgtg ttactacaac
      181 caccgttgtc ccctgctcgt tgatctcctc catcagcttc ataatctccc atgcattatt
      241 cgcatcaaga ttaccggtag gctcgtccgc cagcagaatc ttcggcttgt taatcagcgc
      301 gcgcgcaatc gccacacgct gctgctcccc tccggaaagc tgtctcgggt atgagcggta
      361 ttttgccgcc agtcccacca tcgacaacag ttttggaaca tttttcttga tggagcggtt
      421 ggattcgcca atgacctttt gtgcaaaggc tacattgtca taaatatttc tatcctttaa
      481 caatcggaaa tcctgaaaaa ccaccccaat gttgcggcgg aatttcggga tttgtcttcg
      541 tctgatttta tttaattttc tgccattgat tgtaatggtt ccctccgtcg gttccagttc
      601 cttcaacagg agcttgatca gcgtcgactt gcccgaaccg ctgtcaccga caacaaatac
      661 aaactctccc tcctcaatgt taagactcac gtcattaagt gcaggaattc ccgcagagta
      721 cgacttgctg acatgctcta actttatcat gaaattacct catttctgta tgcatctccg
      781 atggttccgg agaatcaaat gctctgtccc atcccatata gtctactcaa tgtatgcagt
      841 tgttgcaata cccgtatgca aattttaaga attagtagtc cagagactcc atatacttca
      901 tatattttac gaccatcagt gcgatcttga acgtgatggc atcctcgaac acacgcagat
      961 ccagtccggt gctcttctgc agcttatcca gacggtacac taacgtgttg cggtggatgt
     1021 aaagctgtct ggaagtctct gatacgttca ggctgttctc aaagaacttg ttgatcgtcg
     1081 tcagcgtctc ctcatcgaaa tcatccgggg acttattgtc aaaaatctcc ttgatgaaca
     1141 tcttgcacag cggaatcggc agctggtaga tcagacgacc gataccgagt gtggagtaag
     1201 cgattacatc cttctcctcg aagaagatct tgccgacatc aagtgccatc cgcgcctcct
     1261 tgtaggatct ggatacctct ttgatctcgc cgacaatcgt tccgtatgcc acatgaacgt
     1321 cgctgtccgc atccgcacgg aacaggttga ccatgctctc cgcgatcttg ttcagctcct
     1381 cgtaaccttc gttctcgccg acttccttca caacgataat attcttctcg tcgactgctg
     1441 tgacaaaatc cttcgtcttc gtgccgaaga ttccgcggat cttctccagt tcattgccgt
     1501 ccttgtcgcg gttcgtctcc acaatgaata ccacgcggcg cacctcggtg tcgatgtgaa
     1561 gcttcttggc acggttataa atatcaacca gcaacaggtt gtcgagcagc aggttcttga
     1621 taaaattatc cttatcgaac cgctccttat atgctaccag aagattctgg atctggaaac
     1681 tggcaatctt tcccaccatg tatacatcat cgctgtctcc gcttgccaga agaatatact
     1741 caagctggtg ctcatcaaat accttgaaaa actgatatcc gttcaccacc tggctgtctg
     1801 ccggtgactc cacgaacgcc tgcgccggct caatgtactg atccgcctcc ggaaaagtgg
     1861 ctgctaacac cttcccctcc acatcaataa tgcacagatc tattctggtg attcccttta
     1921 atccatcgat tgtattctga agtatttgat ttgaaatcat aagctccgct ccttctcatc
     1981 ctgcttctgc tgaaactctt ctgtccggtc actgtgtttc ttccctatga gcaactttca
     2041 caataaaaag ccgcctttct acctcggaac aaaaatatct aagctatatt ttaatgcaaa
     2101 acagcaaaaa aagaaagagg aaatgcattt tttttatgca tttcctcttt ttcactccgc
     2161 ttccccgaac ttttcctgat cctgatactt gttcatactg ttcttcaatc ttcccagatg
     2221 cagccggttc ttaacgaaac ttccaagtcc atgctttttt ccatgaacgc cgagttctcc
     2281 catgccgaac catatggtcg cgctcatctt atccttatgc tccgcgaaaa aatgttcctg
     2341 cagcggagac ggcagaatcg aaacgatcac atccggcgtc gtcgcattca tctcattgat
     2401 gacggcatcc agattcccca cacaattttc cagtgcatat tctcccgcca gaatcagctt
     2461 cgggaatgct tcctgcagtt ccgctttgac cttctgcacg ccctcctccg tctgtcccag
     2521 cacaaaaatg ctccgcttat tgcgctcgat cctccgatag aattcatatg aaaaatcatt
     2581 ctgctc
//
LOCUS       DBMDQJ010000343         2586 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14115_length_2586_cov_1.269215, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000343 DBMDQJ010000000
VERSION     DBMDQJ010000343.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14115_length_2586_cov_1.269215"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            60..1157
                     /locus_tag="ACZO9R_07575"
     CDS             60..1157
                     /locus_tag="ACZO9R_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR0912959.1"
                     /translation="MKLKTIGFIGLGLIGGSIAKRLRILHKDLVIIATAGHAATIEAA
                     YKEGLTDNDSPCELSDFYDCDYIFLCTPVQKNMEYLRLLKGHVCDRCIITDVGSVKTD
                     IHREVTALGMEAQFIGGHPMAGSEKTGLANAREFLLENAYYILTPTAQTDPAALEDFK
                     ELVASLGAIPMVLDYEQHDYATAAISHLPHIIAYSLVNLVKSCDDCEGTMKTIAAGGF
                     KDITRIASSSPVMWENICLSNQEQILRLIDSFCGELGHMRSAIAASDSKALMEAFTSA
                     KDYRDSLTLHANGTLKQVYELYMDLLDEAGGIATVATILASHHLSIKNIGIIHNREFE
                     DGVLHLEMYDSESLAAAITLLKKHHYTIYER"
     gene            complement(1232..>2586)
                     /locus_tag="ACZO9R_07580"
     CDS             complement(1232..>2586)
                     /locus_tag="ACZO9R_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HIR0912960.1"
                     /translation="DITGAKALIGFFLVKAGGALIDNMAWLFVIGVGVGMADDNDGTA
                     ALAALASWLMITNLLSTGVVTTLMPSIAENADKTLAFDKIANPFIGILAGIIGATCYN
                     KFKGTKLPDWLAFFSGKRCVAIVSGVVSIVVSAILLFVWPVVFGALIALGQAIVGMDA
                     VGAGIYAFLNRLLIPTGLHHALNNVFWFDTIGLGDLANFWKGKTSADVSWDLGMYMSG
                     FFPCMMFGIPGAALAMVHTAKSNKKKIAIGLVGSAALCSFICGVTEPFEFGFMFLAPA
                     LYVVYALLYGIFTFITVLVGFRAGFSFSAGATDLIFSASLPAAKNTWMILPLGIAAFV
                     VFYVVFRFMITKFDLKTPGREDDDDDAEKGAKLENNDYTEVARIVLEGVGGKENIESI
                     DNCITRLRLEIRDYTKVDEKKIKSAGVAGVVRPSQKTVQVIIGTQVQFVADEFKKLCK
                     "
BASE COUNT          695 a          699 c          578 g          614 t
ORIGIN      
        1 tacaatacgt tcagacactc tggttttcct atactgccag agcacggaaa ggcaaaatta
       61 tgaagcttaa gacaatagga tttatcgggc ttggactgat cggcggttcg attgccaagc
      121 gccttcgtat tctacataaa gatctggtca ttatcgctac agccggtcat gccgcgacga
      181 tagaggccgc ttacaaagag gggctgaccg acaacgacag cccttgtgaa ttgtcagatt
      241 tctatgactg cgactatatt tttctgtgca cgcctgtcca gaaaaacatg gaatatctgc
      301 gtcttttgaa gggacacgtc tgcgaccgct gcatcattac cgatgtgggc agcgtcaaga
      361 ccgacattca ccgggaagtg accgccctcg gcatggaggc gcagtttatc ggcggacatc
      421 cgatggcagg ctccgagaag accggtcttg ccaatgctag ggaattttta ttggaaaatg
      481 cttactatat tcttaccccg accgcccaga ccgatcccgc tgcgttagag gactttaagg
      541 agcttgtagc ttctctcggc gcgataccga tggtgctcga ctacgagcag cacgattatg
      601 ccactgccgc catcagccat ctgccccaca tcatcgccta cagccttgtc aaccttgtga
      661 aaagctgcga tgactgtgaa ggaaccatga aaaccatcgc cgccggcggc tttaaggaca
      721 ttacacgcat tgcctcctcc tcccccgtca tgtgggaaaa tatctgtctt tccaatcagg
      781 aacagattct aagactgatt gactctttct gtggggagct tggtcatatg cgctctgcca
      841 tcgccgcttc cgacagcaaa gcgctgatgg aggcctttac ctcggcaaaa gactaccggg
      901 attccctgac ccttcacgcg aacggcacct taaagcaggt atatgagctg tacatggatc
      961 ttctggatga ggcgggcgga atcgccacgg ttgccaccat tcttgctagc caccatttga
     1021 gcatcaagaa tatcggcatc atccacaacc gtgaatttga ggacggcgtg ctgcatctgg
     1081 aaatgtacga cagcgaatcg ctcgccgccg ccatcacact tctaaaaaag caccactaca
     1141 ccatttatga gcgctgaata tatgtctgca tcgaaaaacg accgctttcg cggtcgtttt
     1201 tcattggggc aatctatttt acagaatatg gttacttaca aagtttttta aattcgtcag
     1261 ccacaaactg tacctgggta ccgataatga cctgtactgt tttctggctt gggcgaacca
     1321 cacctgccac accggcagat ttgatctttt tctcatctac cttagtgtaa tccctgatct
     1381 ccagacggag tctcgtaatg cagttatcaa tgctttctat attctctttg ccaccgacac
     1441 cttcaagcac gattcttgcc acttcggtgt aatcattatt ctcaagcttt gcacctttct
     1501 ctgcatcatc atcgtcatct tcacgtcccg gagtctttaa atcaaacttc gtgatcataa
     1561 aacggaatac aacatagaat acgacaaatg ctgcgattcc gagcggaaga atcatccagg
     1621 tgttctttgc cgccggaagg gaagcggaaa agatcaggtc agttgcacct gcagaaaaac
     1681 tgaagcctgc acggaagcct accagaaccg tgataaaggt aaaaataccg tataataatg
     1741 catatactac atagagtgcc ggagcaagga acatgaatcc gaactcgaac ggctcagtaa
     1801 cgccacagat gaatgaacaa agtgctgcgg aaccaaccaa tccgattgcg atctttttct
     1861 tattgctctt tgccgtgtga accatggcaa gtgccgcacc cggaatacca aacatcatac
     1921 acgggaagaa cccggacata tacataccga gatcccatga tacatccgcc gatgtcttgc
     1981 ccttccagaa gttggccaga tcaccgagtc cgatcgtatc aaaccagaat acgttgttca
     2041 atgcatgatg cagaccggtt ggaattaaaa gacggttcaa aaatgcgtag ataccagcgc
     2101 ccactgcatc cattcccaca attgcctgac caagagcgat cagtgcgccg aacacaaccg
     2161 gccatacgaa taacaggata gctgacacga caatcgatac cacaccggat acgatggcaa
     2221 cacagcgttt gccgctgaaa aatgccagcc agtccggaag cttcgttccc ttgaacttgt
     2281 tataacaggt tgctccgatg ataccggcaa gaataccgat aaacgggttt gcaatcttgt
     2341 cgaatgccag cgtcttatcc gcattctctg cgattgacgg catcaatgtg gtaacaacgc
     2401 ccgtcgagag cagattggta atcatcagcc aggatgccag cgccgcaagt gccgcggtac
     2461 cgtcattgtc gtctgccata ccaacgccga cgccgatgac aaacagccat gccatattgt
     2521 caattaaagc gccccccgct tttacaagga aaaatccaat cagcgccttt gcacctgtaa
     2581 tatcac
//
LOCUS       DBMDQJ010000344         2582 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14134_length_2582_cov_1.257960, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000344 DBMDQJ010000000
VERSION     DBMDQJ010000344.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14134_length_2582_cov_1.257960"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            185..997
                     /locus_tag="ACZO9R_07585"
     CDS             185..997
                     /locus_tag="ACZO9R_07585"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HIR0912961.1"
                     /translation="MNHKILFVDLDATLLSDDKTVSEENRAAIQKMLLAGHDIVLSTG
                     RPVESGRAVARELELTQPGCYMIAFNGAVLYDCAADRVLLKRSLPIEVTQELFERAAK
                     AGIYAQTYNNTEIITTRHTAELEYYRKKTGLSYKLSANVLDLLEEEPQKVLMIDLKNT
                     GRLARFREKNLEWEKGKCTSFFSCREYLEYCPLDASKGTGVSCLTNMLYLPLDCTVAV
                     GDEENDIAMLNAAHIGVAVKNATPEVKAAADYVTEHDNNHNAIAEVIERFIL"
     gene            complement(1608..2480)
                     /locus_tag="ACZO9R_07590"
     CDS             complement(1608..2480)
                     /locus_tag="ACZO9R_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR0912962.1"
                     /translation="MEHRIIDLHVHSTESDGTLTPTEIVSAAKAAGLSAFALTDHDTA
                     AGVSKAAPAAAACGIELIPGIELSTEYELPTRAGGGKEVHIVGLYIDPEHPLLIEKTN
                     EFRRCRDARNEKIVAALQREGFSITTEALLAANPDSVITRANIARFLYDSHQIRSVSE
                     AFEKYIGDGCKCYVGRFKVSPMEAVELIHAAGGIAVLAHPLLYHMNQTTLQHLIDELK
                     EVHLDGIEAIYSTYTTGEEQYVKRLAKENGLLISGGSDFHGTNKPTIQIGTGRGHLYI
                     PYSVLETIKEHLRA"
BASE COUNT          647 a          585 c          737 g          613 t
ORIGIN      
        1 agaaaataga cagaagaggg aaagtgcttg tcaattccca gaagcagttc tgcgaggagg
       61 cggttgcggc ggtgccaaag acagatcatg tctaccgcga gcgtgtattt gtcccggcag
      121 aaccggcaga gtaggaacgc tgcaggacat atgatgggct aaaagcagga tgggaggaat
      181 gagtatgaat cacaagattt tgtttgttga tctggatgcg acgctgctct ctgacgacaa
      241 aacagtatcc gaagaaaacc gggccgccat ccagaaaatg cttcttgccg ggcacgacat
      301 tgtactttcc accgggcgtc cggtggagag cggcagggcg gttgcgagag aactggagct
      361 gacgcagccg ggctgctata tgattgcctt taacggtgcg gtactctacg actgcgctgc
      421 ggaccgggtg ctgttaaaac gctcgctccc gattgaggtg acgcaggaac tctttgagag
      481 agccgcaaag gcgggaattt atgcgcagac ctacaataat accgagatta ttaccacaag
      541 acatacggca gagcttgagt attaccggaa aaaaacagga ctttcctaca agctttcggc
      601 gaatgtgctg gatctgctgg aggaggagcc gcagaaggtt ctcatgattg atttaaaaaa
      661 tacggggcgt ctcgcgcggt tccgcgagaa gaatctggaa tgggaaaagg gaaaatgtac
      721 cagctttttc tcctgcagag agtatctgga atactgtccg cttgatgcca gcaagggaac
      781 cggtgtgtcc tgtcttacga atatgctgta tcttccgcta gactgtacgg tagcggtggg
      841 agatgaggag aatgatattg caatgcttaa tgcggcgcat atcggggtag cggtaaagaa
      901 tgccacgccg gaggtgaaag ccgcagcgga ttacgtgacg gagcacgaca acaatcacaa
      961 tgcgatcgcc gaggtgatcg agcgatttat cctgtaaaag gagggactgc tgcatcgggc
     1021 gggcagctgc cttgagtcga taaggaatat tataagaagc acaccacgca gatttcagct
     1081 ggaaatgcag atggaacatt cgtttgagaa aaatgtgagc atttcttcct gctggatgtt
     1141 tgcatcggat tccgtgtctc ttccacgttt ggacacttcc ggagtgtgtc cgacaagtca
     1201 cagacaatga gcggacttcc tggtgtccct aatgatgccg cttcacagac aatataaaac
     1261 cgcgcgaacc gaaactcgtt ggcatctgct attgaaaata cggcagatgc caattcaaac
     1321 atcggcttcg cgcggttatg ttttgttcag cggcatccat aagggacaca cacgaaagtc
     1381 ctaaaattgt ctgctgactt gcggacacag tccgtagagt gttccaaact tgggaagaca
     1441 cggattatcg cggatgcaag cattcggcgg gttagaaatg ccactttttt tgaacagaat
     1501 ttccatttgc attttcaact gaaatcccca agtgtgcctc ttgtaatatt ccttatcgct
     1561 tcaaggcaaa ctggctattc ccgatgcgac agtcctttct tctgccttta tgcgcggagg
     1621 tgttctttga tggtttcaag aacggaatag ggaatgtaga gatgcccgcg tccggtcccg
     1681 atctggattg tgggtttgtt ggtgccgtgg aagtcagagc cgccgctgat gagaagcccg
     1741 ttctcttttg caagccgctt gacgtactgt tcctcgccgg tggtgtaggt cgagtagatc
     1801 gcctcgatgc cgtcaaggtg gacttctttt aactcgtcga tcagatgctg caaggtcgtc
     1861 tggttcatgt ggtagagcag cggatgggct aatacagcga tgccccctgc ggcgtggatc
     1921 agttcgactg cctccatcgg ggagacttta aagcggccga catagcattt gcagccgtcg
     1981 ccgatatatt tttcaaacgc ctccgagacg ctcctaatct gatggctgtc gtagagaaaa
     2041 cgtgcgatat ttgcgcgcgt gatgacactg tcgggattgg cagcaaggag tgcttccgtg
     2101 gtaatggaaa agccttcgcg ctgcagggca gccactattt tttcattgcg cgcatcacgg
     2161 cagcggcgga actcgtttgt tttctcgata aggagcgggt gctccggatc aatgtagaga
     2221 ccgacgatat ggacttcttt tccaccgccg gctctggtgg gcagctcata ttccgtggaa
     2281 agctcgatgc cggggattaa ttcaatacca caggcggcag cggcgggagc agctttagag
     2341 acacctgccg cggtgtcgtg atctgtgagc gcaaaggcgg acagaccggc agcctttgcg
     2401 gcagatacga tctcggtggg agtaagggtg ccgtccgact cggtggagtg gacatgaaga
     2461 tctatgatgc ggtgttccat ggtaaaatcc tctcttcaga taatctaatt cagactaatc
     2521 atagagaaaa aaatcacatt tgtcaaaaaa atatggaaac cagttgaaca cgtagtaaaa
     2581 ta
//
LOCUS       DBMDQJ010000345         2574 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14190_length_2574_cov_1.612616, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000345 DBMDQJ010000000
VERSION     DBMDQJ010000345.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14190_length_2574_cov_1.612616"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            97..996
                     /locus_tag="ACZO9R_07595"
     CDS             97..996
                     /locus_tag="ACZO9R_07595"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR0912963.1"
                     /translation="MTEILKAIIFGIVEGITEWLPISSTGHMILLNEFLTLDVSAQFW
                     DMFLVVIQLGAILAVVVLFWNLIWPFARSSSEAVVAAEQNEKSGSLVKREYWVLGPVT
                     VRMPVIINWCKIVVSCLPAIVFVVLGLDETCDRLFYNPVCVAVALIVFGVAFILVENH
                     NAAKQARVEQMRDITFQMAILIGLFQLLAAAFPGTSRSGATIIGALLIGVSRKAASEF
                     TFILAIPVMLGASLLKLMDFGLHLTGTEWAVLLSGMVSAFLVSVVVIKFLLGYIRKHD
                     FKVFGWYRIGLGVVVLLYFFLLK"
     gene            1006..2562
                     /locus_tag="ACZO9R_07600"
     CDS             1006..2562
                     /locus_tag="ACZO9R_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR0912964.1"
                     /translation="MSILNVEHLTHGFGDRAILDDVSFRLLKGEHIGLVGANGEGKST
                     FLNIVTGKLMPDEGKVEWAKNVRVGYLDQHSTLTAGMTIESVLKSAFSWLFALEEKMN
                     QICDALGEADESQMDAMMEELGVIQDTLTMHDFYTIDAKVEEVARALGLLELGLHHDV
                     TDLSGGQRMKVLLAKLLLEKPDILLLDEPTNYLDAEHIAWLTRYLQEYENAFILISHD
                     IPFLNDVVNIIYHMENQHLDRYVGNYDKFEEVYAVKKAQLEAAYRKQQQEISELKDFV
                     ARNKARVSTRNMAMSRQKKLDKMDVIELAAERPKPEFSFPVGRTPGRYIFETKDLVIG
                     YEEPLSKPLSIALERGHKVALIGANGIGKTTLLKSLLGLIPALGGGVEQGENLMVGYF
                     EQEIKGENKRTCIEEIWEEFPAFSQYEVRAALARCGLTTKHIESQVRVLSGGEQAKVR
                     LCKLMNRETNVLLLDEPTNHLDVDAKEELKRALKEYRGTVLLICHEPEFYQDIVGEVW
                     DCSKWTTKIL"
BASE COUNT          591 a          552 c          822 g          609 t
ORIGIN      
        1 accggctgag ggtggcatga catgtctgca tggtaccggc tgagcgtggc atggcagcgg
       61 ctgatgcata gcagttatga gaagaatgga gtatttatga cagagatttt aaaggcaatt
      121 atatttggta ttgtagaagg aattacggag tggctgccga tcagcagcac cggacatatg
      181 atcctcttaa acgagtttct gacactggat gtaagcgcac agttctggga tatgtttctg
      241 gtagtgatcc agctgggagc gattttggcg gtcgtggtgc tgttctggaa tctgatctgg
      301 ccgtttgcac gcagttcctc ggaagcggtc gtggccgcag aacagaatga gaagagtggt
      361 tcccttgtaa aaagggaata ctgggtgtta ggtcccgtca cggtgcgcat gccggtcatt
      421 atcaactggt gcaagatcgt ggtgtcctgc ctgccggcaa tcgtatttgt tgtgctgggg
      481 ctggatgaga cctgcgacag actgttttac aatccggtgt gtgtggcggt ggcattgatt
      541 gtgttcggtg ttgcctttat tctggtggag aatcacaatg cagcaaagca ggcgcgtgtg
      601 gagcagatgc gggacattac gtttcagatg gcgattctga tcggtttgtt ccagctgctc
      661 gcggctgcgt ttccgggaac gtcgcgctcc ggtgcaacga ttatcggtgc gctcttaatc
      721 ggtgtgtcaa ggaaggctgc gagtgagttt acgttcattc tggcgattcc ggtgatgctc
      781 ggggcaagcc tgctaaagct gatggatttc ggacttcatc tgaccgggac ggagtgggcg
      841 gttctgcttt ccggtatggt cagcgcgttt ctggtgtcgg ttgtcgtcat caagttcctg
      901 ctcggatata tcagaaagca tgactttaaa gtgtttggct ggtaccgcat tgggctcggc
      961 gtggtggtgc ttttgtactt ttttctgctg aaatagaggt gatggatgag tattttaaac
     1021 gtagaacatt tgacgcatgg attcggtgac cgtgcgatac tggacgatgt gtccttccgc
     1081 ctgttaaagg gggagcatat cggtcttgtc ggagcgaacg gcgagggaaa gtcgacgttt
     1141 ctaaatatcg ttacgggaaa gctgatgccg gacgagggaa aagtggagtg ggcgaaaaat
     1201 gtgcgggtcg gttacctcga ccagcactcg acactcaccg cgggaatgac gattgagagt
     1261 gtcttaaagt cggcattttc gtggctgttt gcgttggaag agaagatgaa tcagatctgt
     1321 gatgcgctcg gcgaggcaga cgagtcgcag atggatgcca tgatggaaga actcggcgtg
     1381 atacaggaca ccctgacgat gcatgatttt tatacgatcg acgcaaaggt ggaggaggtt
     1441 gccagagcgc tcggactctt agaactcggg ctgcaccatg atgtgaccga tctaagcggc
     1501 gggcagcgca tgaaggtgct gctggcaaag cttcttctgg agaagccgga cattcttctg
     1561 ctggacgagc cgacgaacta tctggacgcg gagcatatcg cgtggctgac acgctatctg
     1621 caggagtacg agaatgcgtt tatcctgatc tcgcacgaca ttccgttttt gaacgatgtt
     1681 gtcaatatca tctaccacat ggagaaccag cacctcgacc gctatgtcgg caactacgac
     1741 aagtttgagg aagtctacgc ggtaaaaaag gcacagcttg aggcggcata ccgcaaacag
     1801 cagcaggaga tcagtgaatt aaaagatttc gtggcgcgca ataaggcgcg cgtatccacc
     1861 cgcaacatgg cgatgtcgcg gcagaaaaag ctggacaaga tggacgtcat cgagcttgcg
     1921 gcggagcgcc cgaagccaga gttttccttc ccggtgggac gcacgcccgg cagatatatt
     1981 tttgagacga aggatctggt gatcggctat gaggagccgc tctcgaagcc gctttcgatc
     2041 gcactggaac gcggacataa ggtggcgctg atcggtgcaa acggaatcgg caagacaaca
     2101 cttttaaaga gtctactagg tctgattccc gcgcttggcg gcggtgtcga gcagggcgag
     2161 aacctcatgg tcggctactt tgagcaggag atcaaggggg agaacaagcg cacctgcatt
     2221 gaggagatct gggaggagtt cccggcattt tcccagtatg aggtgcgtgc ggcgcttgca
     2281 agatgcggtc tgaccacaaa gcatatcgag agccaggtga gagtcttaag cggcggcgag
     2341 caggcgaagg tgcggttgtg taaactgatg aaccgagaga ccaacgttct gcttttagac
     2401 gagccgacca accatctgga tgtggacgcg aaggaagaat taaagcgtgc gctgaaggag
     2461 taccgcggaa cggttctgtt aatctgccat gagccggagt tttaccagga tatcgtggga
     2521 gaggtctggg actgctcgaa gtggacgacg aagattctct gataagaggc cggc
//
LOCUS       DBMDQJ010000346         2568 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14235_length_2568_cov_1.610863, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000346 DBMDQJ010000000
VERSION     DBMDQJ010000346.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14235_length_2568_cov_1.610863"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="ACZO9R_07605"
     CDS             <1..1274
                     /locus_tag="ACZO9R_07605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0912965.1"
                     /translation="ELMIAGFVFLSAELVMLLARASSREWEVAGLLVASGTISFLIDT
                     LFLIFYLSMVRRLKAEVMWENSLVCWLGRGIEKTFRERSVTVKILMIFAIHMIVCFFL
                     AAGAFYYRNVPALIALLLFSAMEAYILLRKSVEHYQILQGVEEIADGDLSAKIDVEEL
                     HGEDRKLAETINNIGAGLLHAVDDSTKNERMKADLITNVSHDIKTPLTSIINYVNLIQ
                     REDVDNERVKNYVKILDEKSQRLKQLTEDLVEASKVSSGNVKLEMHKIDIVELVCQTG
                     GEFNEKFEDKELTIVTKLPRQAVYIWSDGRHLYRVIENLYNNVAKYALQKTRVYVEVQ
                     ADEERAVFSIKNVSARSLARENSEVEDLTERFIRGDSSRTTEGSGLGLSIAKNLTLLM
                     GGMFDISVDGDLFKATIALPLYVEGQEERGQ"
     gene            1276..1935
                     /locus_tag="ACZO9R_07610"
     CDS             1276..1935
                     /locus_tag="ACZO9R_07610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR0912966.1"
                     /translation="MVKAVIFDMDGVLIDTEKWLNKYWRQAAAEAGYDMTVEDGLAIR
                     SLAAKYAAPFLQERFGADFPYWTIRERRKELMKAHIAANGIDKKPGVDELLDYLRAHR
                     IKTAVATATDPARTKDYLTRIGIYDKFDRIVCATMVENGKPKPDIYLYACEQIGEAPA
                     DCIAVEDSPNGVRSATDAGIRTVMVPDLAEPDADAAKRIAAKAGTLFDIILMFEDGDL
                     S"
     gene            2016..>2568
                     /locus_tag="ACZO9R_07615"
     CDS             2016..>2568
                     /locus_tag="ACZO9R_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR0912967.1"
                     /translation="MKLYDSGVYLVNGTELVADDAQAAAAIKSRTGREVAKAEAAQGT
                     IAYGILKDHNVSGNMDKLQIKFDKLTSHDITFVGIIQTARASGLEKFPIPYVLTNCHN
                     SLCAVGGTINEDDHMFGLTCAKRYGGIYVPPHQAVIHQFAREMLAAGGKMILGSDSHT
                     RYGALGTMAVGEGGPELVKQLLSK"
BASE COUNT          653 a          574 c          784 g          557 t
ORIGIN      
        1 ccgaacttat gattgctggc tttgtgtttt tatcggcgga gctggttatg ctgcttgcga
       61 gagcaagcag ccgggaatgg gaggttgcag gtctccttgt cgcatcaggg accatcagtt
      121 ttctcattga tacgctgttt cttatcttct atctgagtat ggtgcgtcgc ctgaaagcgg
      181 aagtgatgtg ggagaacagc cttgtgtgct ggcttggacg cgggattgag aagacgttcc
      241 gggaaaggtc tgtcacagtt aagattttga tgatctttgc gattcatatg atcgtctgct
      301 ttttcctggc ggcgggagcg ttttactaca gaaatgtgcc ggcactcatt gccctgctct
      361 tattcagcgc catggaggca tacattctgc tccggaaatc ggtggagcac tatcagatcc
      421 tgcagggagt tgaggagatt gcggacggtg atctctctgc caagattgac gtcgaagaac
      481 ttcacggtga ggaccggaag ctggcggaga cgatcaacaa tatcggcgcc ggactgttgc
      541 atgccgtgga cgacagcacg aaaaacgagc gcatgaaagc ggatctgatt accaacgtct
      601 cccatgacat caagacaccg ctgacatcga ttatcaatta tgtcaacctt atacagcgcg
      661 aggatgtgga caacgagcgc gtgaaaaact acgtgaagat cctggacgaa aagtcccagc
      721 gcttaaagca gctcacagaa gatctggtgg aggcatccaa ggtcagttcc gggaatgtga
      781 agcttgagat gcacaagatc gacattgtgg aactggtgtg ccagactggc ggcgaattca
      841 atgaaaagtt cgaggacaaa gaacttacga tagtgacaaa gcttccgcgc caggcggtct
      901 acatctggtc agacggacgg cacctctacc gcgtgatcga gaatctctat aataatgtcg
      961 ccaagtatgc acttcaaaag acgcgtgtct atgtggaagt gcaggcggac gaagaacgtg
     1021 ccgtcttttc tattaagaat gtttcggcgc ggtcactggc aagagagaac agcgaagtgg
     1081 aagatctgac ggaacggttt atccgggggg attcctccag aacgacggag ggaagcggtc
     1141 tgggactttc catcgcgaag aatctcacgc ttcttatggg aggaatgttt gatatttctg
     1201 tggatggaga cctgtttaag gcgaccatcg ctcttccgct ctatgtggag ggacaggaag
     1261 aaagggggca gtgatgtggt aaaagcagtg atctttgata tggacggggt actcattgac
     1321 acggaaaaat ggctgaacaa atactggcgc caggcggcag cggaggcggg gtatgatatg
     1381 acggtggagg atggtcttgc gatccggagt ctcgcggcaa agtatgcggc gccgtttctg
     1441 caggagcggt ttggagcgga tttcccgtac tggacgatcc gcgagcggcg caaggaactg
     1501 atgaaggcgc acatcgcggc gaacgggatc gataaaaagc cgggtgtgga tgaacttctg
     1561 gactatctgc gggcgcatcg gattaagacg gcggtggcaa ctgccacaga tccggcgcgc
     1621 acaaaggatt atctcaccag aatcggcatc tacgacaagt ttgaccgtat agtgtgcgca
     1681 acgatggtgg agaacggcaa gcccaagccg gatatttatc tctatgcctg tgagcagatc
     1741 ggggaagcgc ctgcggactg tattgcggtc gaagattccc ccaacggcgt gcgctccgca
     1801 acggatgcgg gaatccggac ggtcatggtg ccggatctgg cggaaccgga tgcggatgcg
     1861 gcaaaacgca tcgcggcaaa agcgggtaca ctctttgata tcatactgat gtttgaagac
     1921 ggcgatttgt catgataaag cgcgaaagtg cttgattttt acctgcaaga ccgatataat
     1981 ttttgaaagg ctatgataag ggcaaggagg aaatcatgaa gttatatgac agcggagtat
     2041 atctggtaaa cggaacggaa ctggttgcgg atgacgcgca ggcagctgcc gcaataaaga
     2101 gcaggacggg cagagaggtg gcaaaagccg aggcggcgca gggaacgatc gcatacggca
     2161 ttttaaagga tcacaatgta tccggcaata tggacaagct gcagatcaag tttgataagc
     2221 tgacttccca cgacattacg ttcgtaggaa tcatacagac agcacgtgca tcgggactgg
     2281 agaagttccc gattccgtat gtactgacga actgccacaa cagcctctgc gcagtgggcg
     2341 gcaccatcaa cgaggatgac cacatgtttg ggctgacctg tgcaaagcgc tatggcggaa
     2401 tctatgtgcc gcctcatcag gcagtcattc accagtttgc tagagagatg ctggcggcag
     2461 gcggcaagat gatcctcgga tcggattccc acacgagata cggagcgctc ggtaccatgg
     2521 cagtcggcga gggcggtcca gagctggtaa agcagcttct ctccaaga
//
LOCUS       DBMDQJ010000347         2564 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14264_length_2564_cov_1.384896, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000347 DBMDQJ010000000
VERSION     DBMDQJ010000347.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2564)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14264_length_2564_cov_1.384896"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="ACZO9R_07620"
     CDS             complement(<1..119)
                     /locus_tag="ACZO9R_07620"
                     /inference="COORDINATES: protein motif:HMM:NF023880.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose acetyltransferase domain-containing
                     protein"
                     /protein_id="HIR0912968.1"
                     /translation="MTEREKCQLGLLYDTTFPGRDEDNLRCLDLCHEFNQMKPS"
     gene            complement(130..1005)
                     /locus_tag="ACZO9R_07625"
                     /pseudo
     CDS             complement(130..1005)
                     /locus_tag="ACZO9R_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856403.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
     gene            complement(1032..2087)
                     /locus_tag="ACZO9R_07630"
     CDS             complement(1032..2087)
                     /locus_tag="ACZO9R_07630"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR0912969.1"
                     /translation="MKIVVLAGGLSTERDVSFKTGEMVTKALRENGHQVILLDVFMGY
                     SDKEEDLTGIFDRAEAVSVKVAAIPETAPDLEKVKAQRKDQSDNFFGPNVIELCRMAD
                     IVFMALHGENGENGKIQAAFDLFGIRYTGTGYLGSALAMNKGMAKQLFLENGIPTPRG
                     TSLKRGEDAAKIETCGIHFPCVVKPCSGGSSIGVSIVHDKAEYEQALKEAFRWENELV
                     IEEYVKGREFSVGVIDFQALPIIEIAPVEGFYDYKNKYKAGSTVETCPAELSEQITKE
                     MQGYAEKVAEVLGLNTYSRTDFLLDAEDHIFCLEANTLPGMTPTSLLPQEAKVTGVDF
                     NQLCEKLIESSMRKYKN"
     gene            complement(2212..>2564)
                     /locus_tag="ACZO9R_07635"
     CDS             complement(2212..>2564)
                     /locus_tag="ACZO9R_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912970.1"
                     /translation="ALRVREVEKLIMCETKEKGSSRKSYGHTNVLVDMMQNYPGFMDY
                     IAYQYPLEGERIELLEQILTALAVGRKLEAEQMIYHYGSMIHGRDLIARQVFLWLLGM
                     AEYSRRECIAEERR"
BASE COUNT          528 a          797 c          517 g          722 t
ORIGIN      
        1 gacggcttca tctgattgaa ttcgtggcac agatcgaggc agcggagatt gtcctcatcc
       61 cggccgggaa acgttgtatc atacagcagc ccgagctggc atttttctcg ttcggtcata
      121 ttacgtcacg atctcaaact gctgctccgg cgtgtgaatg accgccttcg ttccctcaag
      181 gccgatcgcc tccacatcgg tcgcgtacac ttccttttcc gcgagccgtt tgctgccggt
      241 ctctgtctct gccgtctttg cttcctgtcc gattccatag aacaccgccg gtctgccatt
      301 caccatcttt ttgtcacaga gcttatcgtc atccccgttt aatacggcga tcccgtccgg
      361 tgccagatgc tcaaaactct ccgtcttcgc ctttaaaatg ccgtctcttg tgatgaggtt
      421 ctccagatga cataagccga tattggtaat gacgcagata tccggctgtg ccatctctgc
      481 aagacggtgc atctcaccga actcagagat tcccatctcg agcaccgcca cctgatgttc
      541 ctcccggatc ttaaagatcg tcagcggaag tccaatctca ttgttcagat tcccctcggt
      601 ctttaacacg cggtatttct cggagagcac cgacgcaatc atctcttttg tgctggtctt
      661 tccaacgctt cctgtgatcc ccacgacttt gatgtcgagt gagcggcggt agaacgccgc
      721 gatcttcttc atggcttcct ccgtagagtc aaccagaata tacggtcctg ccggatgctc
      781 gagccgctgc tcggagagca cggcaagcgc tcccttttca aaaaccgccg gaatgaaatc
      841 atgtccgtcg acgcgcgctc cgcggatggg aataaacaga tatccctccc cgaccagacg
      901 gctgtcaatc accgccccct gaatctcctt tttgcggtcg cactcttccc cgatgtatgt
      961 accgccgcac gcctgcgcga tatgctccaa tgtcatattt ttcataatgg ctcccatttc
     1021 agacttcatt cttaattctt gtacttgcgc atggaactct cgatcagctt ctcacagagc
     1081 tggttgaaat ccacgccggt cacctttgcc tcctgcggca gcagactggt cggcgtcatg
     1141 cccggaagtg tgttcgcctc cagacagaaa atgtgatctt ccgcatccag aaggaagtct
     1201 gtgcgcgagt aagtgttgag tccaagcacc tctgccacct tctctgcata gccctgcatt
     1261 tcctttgtaa tctgttcgga cagctccgcc gggcaggtct ctaccgtact tcccgctttg
     1321 tatttgttct tataatcata gaagccttcc accggcgcga tctcgatgat cgggagcgcc
     1381 tgaaaatcga tgacgccgac agaaaattct cttcctttta catattcctc aatgaccagc
     1441 tcattctccc agcggaatgc ctcctttaat gcctgctcgt attctgcctt gtcgtgcacg
     1501 atcgatacgc cgatgcttga tccgccgcta cacggcttta ccacgcacgg gaaatgaatt
     1561 ccacaggtct cgatctttgc cgcatcctcg ccacgcttta aggacgtgcc tctcggcgtc
     1621 gggattccat tctcgaggaa cagctgcttt gccattcctt tgttcatggc aagtgcactg
     1681 cccagatatc cggttcctgt gtagcggatg ccaaacaggt caaacgccgc ctggatctta
     1741 ccgttctctc cgttctctcc atgaagcgcc ataaacacga tgtccgccat ccggcaaagc
     1801 tcgatgacat tcggtccgaa gaaattatcc gactgatcct ttctctgcgc ctttactttt
     1861 tccaggtccg gcgcggtctc cggaatcgcc gccaccttga cgctgaccgc ctcggctctg
     1921 tcaaaaattc cggtaagatc ttcttcctta tcgctatatc ccataaaaac atcaagtaaa
     1981 attacctgat gtccgttctc tctgagtgct ttcgtaacca tctctcctgt cttgaaggac
     2041 acatctctct ctgtgctgag cccccctgcc aaaacaacta tcttcatggt atccgtctct
     2101 cctttttcaa aacttttctc gctttatagt ataccttttt cgatctgatg acaagaatat
     2161 tccttgtttt ttcaaagctc tgcccctgcg ctgcgacccc tttcctgctt tttatctacg
     2221 ctcttccgct atacactcac gtctgctgta ttctgccatc cccaaaagcc acagaaaaac
     2281 ctgcctcgca atgagatccc tcccgtgaat catactgcca taatgataaa tcatctgctc
     2341 cgcttccagc tttcttccca ccgcaagcgc ggtcagaatc tgttccaata attcaatccg
     2401 ttctccctcc aatgggtact gatatgcaat ataatccata aaccccggat aattctgcat
     2461 catatccacc aatacatttg tatgtccgta gcttttccta cttgagccct tttcttttgt
     2521 ttcacacatg attaactttt ctacctctcg cacacgcagg gcac
//
LOCUS       DBMDQJ010000348         2562 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14281_length_2562_cov_1.078423, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000348 DBMDQJ010000000
VERSION     DBMDQJ010000348.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14281_length_2562_cov_1.078423"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="ACZO9R_07640"
     CDS             <1..1754
                     /locus_tag="ACZO9R_07640"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016438291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HIR0912971.1"
                     /translation="ALAATQEEREKSLELDIGYDSAEQTWEVIVKFVGTTEELEALLF
                     KNFPEEYPRIQLTNLQNEYAVLVLPEHLVEPVAALDKIEYMEKPKLLFFAVDNGKRAS
                     CITQLQERTGLSGAGVLMAVIDSGIDYAHPDFRRADGTTRIVSLWDQTIPASGGLSAP
                     AGYFLGTEYDEARINEALAQPTEQQRYAVCPSRDASGHGTHVAGIAAGNGRASEGRYR
                     GVAYESDLLIVKLGNQREGAFPRTTELMQAVDYCIRKAQERQQPVAINLSFGNNYGSH
                     TGTSLIETYLDDLANYWKSSIVIGSGNEGAAATHTAGALGMGQAENVEFAVSAYESAL
                     NLQIWKSYADEMAVLLIHPGGMQIGPIRQIQGPQRFTLGQTQILLYYGEPSPYSPYQE
                     IYLDFLPVRDYVDSGIWTVRLIPQRIVTGRYDMWFPAGGVLNEGTGFLLPREETTLTI
                     PSTAAKVITVGAYDARFGQLAGFSGRGFTGNGQVKPDLAAPGVEITSCAPGGGYTARS
                     GTSMATPFVTGSAALLMEWGIVRGNDAYLYGEKIKAYLIRGARPLGGGRASGLLQEYP
                     NPQVGWGTLCLSDSLPE"
     gene            1995..>2562
                     /locus_tag="ACZO9R_07645"
     CDS             1995..>2562
                     /locus_tag="ACZO9R_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HIR0912972.1"
                     /translation="MNDIVYQHEVIVPDRGLPFKLFLFEGGGGKYIREKHWHRSIEIF
                     AVRQGQLDFFLNEKKYVLAAGEFVLVNSNEVHAIHAPLPNETIVLQIPLGVFADYYTE
                     EQFIWFSHSGKEDDRQVFSLLETMFVAYGEKQTGYELKMLSCFYQLEYLLVTRYRKLE
                     VGEEILKNNKQLKRLGRITGYLKEHYTED"
BASE COUNT          637 a          544 c          823 g          558 t
ORIGIN      
        1 tggcactggc ggcgacacag gaggagcggg agaagtcgct ggagctggac atcggctacg
       61 atagtgccga acagacgtgg gaggtcattg taaaatttgt cgggacaacg gaggagcttg
      121 aggcgctcct ttttaaaaat tttccggagg aatatccgcg gattcaactg acaaatctgc
      181 agaatgaata tgccgtttta gtgctgccgg aacatctcgt ggagccggtg gcagcgctgg
      241 ataaaatcga gtacatggaa aagccgaaac ttttattttt cgcggtggac aatggaaagc
      301 gtgcatcctg cattacgcag ctgcaggaga gaaccggcct gtcgggtgcc ggtgtcctta
      361 tggcggtcat tgattccggc atcgattacg cgcatccgga ttttcggagg gcggacggta
      421 cgacccgcat tgtcagtctc tgggatcaga cgatcccggc gtccgggggc ttgtcggcgc
      481 cggcggggta tttccttgga accgagtacg atgaggcgcg catcaacgag gcgctcgcgc
      541 agccaacgga acagcagcgg tatgcggtct gcccgagcag ggatgcatcg ggacacggga
      601 cacacgtggc gggaattgcc gccggcaacg gaagagcgtc ggagggaaga taccgcggtg
      661 tggcgtatga gagcgatctt ttgattgtaa agctcggcaa ccagcgggag ggggcgttcc
      721 cgcggacaac ggaactgatg caggcggtgg attactgcat tagaaaagcg caggagagac
      781 agcagccggt cgccatcaac ttaagctttg ggaataatta cggaagccac acgggaacgt
      841 cgctcattga gacttatctg gatgatctcg caaactactg gaagagctcg atcgtgatcg
      901 gaagcgggaa tgagggagcg gctgccacgc acacggcggg agcgcttgga atggggcagg
      961 cggaaaatgt agagttcgcg gtgagcgcgt acgagagcgc cttaaatctt cagatctgga
     1021 aatcctatgc ggatgagatg gcggtgcttc tcattcatcc gggcggcatg cagatcggac
     1081 cgatccgcca gatacaggga ccgcagcgtt ttacgctggg gcagacgcag attctgctct
     1141 actatggaga gccgagccca tacagccctt accaggagat ctacctggat ttccttcccg
     1201 tgcgcgacta tgtggacagt ggaatctgga cggtgcgcct gattccgcag cgcatcgtta
     1261 cggggcgcta tgatatgtgg ttcccggcgg gaggcgtttt aaatgagggc accggatttc
     1321 ttctgccgcg ggaggagacg acgcttacca ttccgtccac tgcggcaaag gtgattacgg
     1381 tcggggcgta cgacgcgcgg ttcgggcagc tggcgggatt ttcgggcaga ggatttacag
     1441 gaaacggaca ggtcaagccg gatctggcag cgccgggcgt ggagatcacc tcctgtgcgc
     1501 cgggtggagg ctatacggcg cgttccggaa cttcgatggc aaccccgttt gtgacgggaa
     1561 gcgcggcgct tctgatggag tgggggattg tgcgcggaaa cgatgcgtac ctgtacggag
     1621 aaaaaataaa ggcgtacctg atccgcggag cgcgtccgct cgggggagga agggcttctg
     1681 gtctgctgca ggaatatccg aatccgcagg tggggtgggg aactttatgt ttgtcggaca
     1741 gtctgccgga gtaggaaaac gggaagaatt ccggaaacgc aaaaaagaga taacatggaa
     1801 attgggcagt atttcaaaga aagataaaca aaatataaat taattgtgaa aaataaatga
     1861 gaaaattgaa atttatgtta tacttttaac aaaataaaaa acatacagta tattttatga
     1921 ggatggctgg tgaaaaccgg cggtttcagc ttactgcgcg gtaatgaaat tttgttaaag
     1981 gagaccgaag tggagtgaat gatattgttt accagcatga agtgatcgtt ccggacaggg
     2041 gacttccgtt taagctgttt ctctttgagg gcggcggcgg aaaatatatc agggagaagc
     2101 actggcaccg ttctatcgag atatttgccg tgagacaggg gcagcttgat tttttcctga
     2161 atgagaaaaa atatgtgctt gcagcggggg agtttgtgct ggtgaatagc aatgaggtgc
     2221 atgcgatcca tgcccctctg ccaaacgaga cgattgtgct gcagattccg cttggcgtct
     2281 ttgcggatta ttatacggaa gaacagttta tctggttttc gcacagcgga aaagaggatg
     2341 accgtcaggt gttttcactg ctcgagacaa tgtttgtggc gtacggtgag aaacagaccg
     2401 gttacgaatt aaaaatgcta agctgttttt atcagctgga gtatcttctg gtgacaagat
     2461 accggaagct tgaggtgggc gaagagatac ttaagaacaa taagcagctt aagcgtctcg
     2521 gaaggattac cgggtatctg aaggagcatt atacggagga tg
//
LOCUS       DBMDQJ010000349         2557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14312_length_2557_cov_1.566775, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000349 DBMDQJ010000000
VERSION     DBMDQJ010000349.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14312_length_2557_cov_1.566775"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            20..826
                     /gene="fliK"
                     /locus_tag="ACZO9R_07650"
     CDS             20..826
                     /gene="fliK"
                     /locus_tag="ACZO9R_07650"
                     /inference="COORDINATES: protein motif:HMM:NF014207.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HIR0912973.1"
                     /translation="MREKKDINIEIGGNIQVAREQAQEQPVLNEKLTAEQFLQKLGRA
                     LAQNGGFGFAGMQKLFAGKEFQTIFRSVIEKQWLLQPEELKQEHKVSGLYERLEQQLS
                     QMEEAVRATGSTQNTFLQTASQVHGNLEFMNQMNQIYHYVQLPLKMSGQNANGELYVY
                     ANRKNLRDPDAELTAFLHLDMEQLGSTDVSVRMQNRSVRTNFYLEDDASYDLVEKHLE
                     VLNKRLKSKGYQSSITVTHEKKDISFKENIVKKGKATVGSLHRYSFDVRA"
     gene            860..1165
                     /locus_tag="ACZO9R_07655"
     CDS             860..1165
                     /locus_tag="ACZO9R_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HIR0912974.1"
                     /translation="MAENKHIAGKEKTAVAVAYNPGEVAPKILAVGKGEVAERIIETA
                     KENDVPFYQDNKLAETLSRLQIGDTIPPELYEVVAEILVFVDDMDRMKGKLKESGLL"
     gene            1231..1626
                     /locus_tag="ACZO9R_07660"
     CDS             1231..1626
                     /locus_tag="ACZO9R_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR0912975.1"
                     /translation="MDRTKGRERGYRMNRRQTGTAYERRAGEYLKEQGYELIGYNFRC
                     RQGEIDIIARDGRYLVFVEVKYRRDGQTGDPLEAVDRAKQRRISRTAQYYCLTHGYGE
                     TTPCRFDVAAVLGTDGEVRLVKHAFEFQG"
     gene            1641..2531
                     /gene="pgeF"
                     /locus_tag="ACZO9R_07665"
     CDS             1641..2531
                     /gene="pgeF"
                     /locus_tag="ACZO9R_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HIR0912976.1"
                     /translation="MTAFVRKPAEQPVLREDWHENEDGSRLLLLKYPLLERTGIVEHC
                     FTTRLGGVSTDIFSTMNLSFTRGDEEAAVRENYRRLARALGAEVGQFVCSDQTHTVNV
                     RRVTAADAGKGLTRERDYRDVDGLITDEPGLVLSTFYADCVPLYVVDPVHRAIGMSHS
                     GWRGTAARMGAVTLSAMQEAYGTRPEDVVCAVGPSICKDCYEVSADVADIFAEEFPGH
                     EQEILAESEKNSVGMAHADKKYQLDLWKANEIIFQEAGVRKEHLAVTDICTCCNPKLL
                     FSHRASHGKRGNLGGFLYLK"
BASE COUNT          675 a          548 c          842 g          492 t
ORIGIN      
        1 atgcaaaggt ggtgaggcta tgcgagagaa gaaggacatc aatattgaaa tcggcggcaa
       61 tattcaagtg gcaagagaac aggcgcagga gcagccggtc ttgaatgaaa aattgacggc
      121 agaacagttt ttgcagaagc tcggcagagc gctcgcacag aacggcgggt tcggctttgc
      181 gggaatgcag aagctttttg ccggaaagga atttcagacc attttccgca gtgtgatcga
      241 gaagcaatgg cttctgcagc cggaggaatt aaagcaggag cataaggtga gcgggctgta
      301 tgagcgctta gagcagcagc tttcccagat ggaggaggcg gttcgggcga ccggaagcac
      361 gcagaatacg tttttgcaga cggcatcgca ggttcacggc aatctggagt tcatgaatca
      421 gatgaaccag atttatcact atgtgcagct gccgttaaag atgtccggac aaaatgcgaa
      481 cggggaactc tatgtctatg ctaaccggaa gaatctgcgc gatccggatg cggaactgac
      541 ggcattttta catctggaca tggagcagct cggctccacg gacgtctcag tgcggatgca
      601 gaaccgcagc gtacgcacca atttttatct ggaggatgac gcctcctatg atctggtgga
      661 aaagcacctg gaagtgctga acaagcgtct taagagcaag ggctaccagt cttccatcac
      721 ggtcacacat gagaaaaaag acatcagttt caaggagaac atcgtaaaga aaggaaaggc
      781 gacggtggga agcctgcacc gctattcttt tgatgtaaga gcataggaaa gcaggattct
      841 ttgaagggag gatgtggcga tggcggagaa caagcatatt gcaggaaaag aaaagacggc
      901 ggtcgccgtt gcctacaatc cgggcgaggt ggcgccgaag atcctcgcag tcggaaaagg
      961 cgaggtggcg gagcgcatca ttgagacggc aaaagaaaat gatgttccgt tttatcagga
     1021 taacaagctg gcggagacct tatccagact tcagatcggg gatacgattc cgccggaact
     1081 ctacgaggtg gtcgcggaga tccttgtctt tgtggacgat atggaccgca tgaagggaaa
     1141 actcaaagaa tccggcttac tgtaggcaga tgacagatgc cgtgatggta acagaaggca
     1201 cgggaagaac ccggacggcg gaacgtggtg atggatcgga cgaaaggcag ggagagagga
     1261 taccggatga acaggcggca gacgggaaca gcgtatgaga gacgggcggg ggagtatctg
     1321 aaggaacagg gatacgagct gattggatac aatttcagat gccggcaggg agagatcgac
     1381 atcatcgccc gcgatggcag atacctggtg tttgtggaag tgaaataccg cagagacggg
     1441 cagacgggcg atccgctgga ggcggtggac cgggcgaagc agcgccgcat cagcagaacg
     1501 gcgcaatatt actgcctgac gcacggatat ggggagacga cgccctgccg ctttgatgtg
     1561 gcggcggtgc ttggaacgga cggggaagta cggcttgtga aacatgcatt tgaatttcag
     1621 ggatgacggg aggatgagac ttgacagcgt ttgtgagaaa accagcagaa caaccggttt
     1681 tgcgggagga ctggcatgaa aatgaggacg gcagcaggct tctgctgcta aaatatcccc
     1741 ttttggagcg cacggggatc gtggagcatt gttttacaac acggctcgga ggcgtcagca
     1801 cagacatttt ttctacgatg aatttaagct ttaccagagg tgatgaggag gcggcggtgc
     1861 gtgaaaatta ccggcgcctg gcgagggcac tcggggcaga ggtggggcag tttgtctgct
     1921 cggatcagac acataccgtc aatgtgcgcc gcgtcacggc agcggacgcc ggaaaaggac
     1981 tgaccagaga gcgggactac cgggatgtgg acggactgat tacggatgag ccgggacttg
     2041 tcctgtcgac gttctacgcg gactgtgtgc cgttatatgt ggtggatccc gtacaccgcg
     2101 ccatcggaat gagccattcg ggctggagag gcacggcggc gcgcatggga gcggtcactc
     2161 tgtctgccat gcaggaggca tacggcacca gaccagagga tgtggtctgc gcggtcggtc
     2221 cgtccatttg caaggactgc tatgaggtga gcgcagatgt ggcagatatt ttcgcggagg
     2281 aatttccggg gcatgagcag gagattctgg cagagagtga gaagaacagc gtcgggatgg
     2341 ctcacgcaga caaaaaatac cagctggatc tctggaaggc gaacgagatc atttttcagg
     2401 aggcgggcgt gcgaaaagag catctggcag tgacggacat ctgtacctgc tgcaatccaa
     2461 agctgttgtt ttcccaccgc gcaagtcatg gaaagcgcgg aaatctgggc ggatttctgt
     2521 acctgaaata aggatgtata agatcgtcct tcaggta
//
LOCUS       DBMDQJ010000350         2557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14313_length_2557_cov_1.390472, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000350 DBMDQJ010000000
VERSION     DBMDQJ010000350.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14313_length_2557_cov_1.390472"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>297
                     /locus_tag="ACZO9R_07670"
     CDS             <1..>297
                     /locus_tag="ACZO9R_07670"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR0912977.1"
                     /translation="YSLKIFYESRSPKLPQGFLLRIGIDDFKNINESHGNEYGDYILR
                     RTAQCIEENIGPEQSLFHIVADEFAVVDFSGGSVQQAEDLFHEIKKSIYAFIEQ"
     assembly_gap    298..307
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <308..1277
                     /locus_tag="ACZO9R_07675"
     CDS             <308..1277
                     /locus_tag="ACZO9R_07675"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR0912978.1"
                     /translation="FNNTFNFNHGWMASVEMNYQSKGDMDNSFENAMKLSEFALSEAK
                     ERGKNSCYVFEEEEYEDYLKSRTLLRQLHHAVNNGFEGFEAYFQPLVNAANGELTGAE
                     TLLRFHSPEGGMVSPGEFIPILEESGLIIPVGRWVLRQALDACREWQKILPEFKVNVN
                     LSYVQVLKSRVLTDVRRIVDEFGVKPSSVCIELTESGYLEENERFDKVWNGLKEYGVL
                     LALDDFGTGYSNLRCLSDLKPAYIKIDRSFTLKALNREYEYRLLNHIVEMSHSLGLHV
                     CVEGIETEGELTKAKQTEPDYIQGFLFGKPCSRAEFTQKFFTKQAG"
     gene            1392..1526
                     /locus_tag="ACZO9R_07680"
     CDS             1392..1526
                     /locus_tag="ACZO9R_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912979.1"
                     /translation="MTETGFLIFMGILLLLVVIVVVVVVASSVAGTAAAIADDDDEEE
                     "
     gene            1659..>2557
                     /locus_tag="ACZO9R_07685"
     CDS             1659..>2557
                     /locus_tag="ACZO9R_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR0912980.1"
                     /translation="MISKFSVKRPYTVIVGVILVIVLGVVSFTRMTTDLLPDMNLPYA
                     LVITTDVGASPEEVETDVTAPVEASMATTSSIKNVSSVSYDNYSMVILEYEQSANMDS
                     ILIEIQQKLDQLEGSFSDSVGKPMIMQIDPDMMPVMVASADVEGMDESEVSSYVENEF
                     SPALESVEGVASVSATGVLEESVQVTMDQDKIDALNDKIQEKIEEQFVDAQKELDDAS
                     AELENGEKKLESGKSELANQVSAAANQIENGKIQAYVGASELKTNMTMMTEAKKIMEK
                     AIPALQQLYNDGIAARQQLEALK"
BASE COUNT          722 a          484 c          753 g          588 t
ORIGIN      
        1 atatagtctg aagatttttt atgagagccg cagtccaaag ctgccacagg gatttttgct
       61 cagaatcggc atcgatgatt tcaagaacat caacgagagc cacggcaacg aatacggaga
      121 ttatatttta agaagaacgg cacagtgtat tgaggaaaat ataggcccgg aacagagcct
      181 gttccatatt gtggcggatg agttcgcagt tgtggatttt tccggcggca gtgtgcagca
      241 ggccgaagat cttttccatg agatcaagaa atccatctac gcatttattg agcagaannn
      301 nnnnnnnttt taataatacg tttaatttta atcacggctg gatggcttcg gtggaaatga
      361 attatcaaag taaaggcgat atggacaatt cctttgaaaa tgccatgaag ctttcggagt
      421 ttgcactcag cgaggcgaag gagcgcggaa aaaacagctg ctatgtattt gaggaggagg
      481 aatacgaaga ttatctgaaa agccgcacac tgctgcggca gctgcatcat gcggtaaata
      541 atggatttga gggatttgag gcatatttcc agccgttggt aaatgctgca aacggtgaac
      601 tgaccggggc ggagacgctg ctccgcttcc attcaccgga gggtgggatg gtatcgcccg
      661 gcgaatttat cccgattctc gaagaatccg gtctgattat tccggtcggc aggtgggtgc
      721 tgcgccaggc gctggatgca tgcagggaat ggcagaagat tcttccggag ttcaaggtga
      781 atgttaacct gtcctatgtg caggtgttaa agagccgtgt tctcacggat gtgcgcagga
      841 ttgttgatga gttcggagtc aagccgtcga gtgtctgcat cgagctgaca gagagcggtt
      901 atctggagga gaatgagcgc tttgacaaag tctggaacgg actcaaagaa tacggtgtat
      961 tgctggcact ggatgatttt ggcacaggat attccaacct gcgttgttta agcgatttaa
     1021 agcctgcata tattaagatt gaccggtcgt tcacattaaa agcgctgaac cgtgaatatg
     1081 agtaccggct tttaaatcat atcgtggaga tgtcccacag cctggggctt cacgtatgcg
     1141 ttgagggaat cgagacagag ggcgaactta caaaggcaaa acagacagag ccggattata
     1201 tacaggggtt tctgttcgga aaaccttgca gcagggcgga attcacacag aagttcttca
     1261 caaaacaggc cggataaagg acttttcatg cgggtgtaaa tatgctatac ttgcatcagg
     1321 agtgagatta tgccggaaga ggtgcggcgg gatgtgcggc atctgctgac agaaagacga
     1381 ggagaaggag aatgacagag acgggttttt tgatctttat ggggatctta ttgctgctgg
     1441 ttgtaattgt ggtcgtggtt gtggtggctt cttcggtagc cggaacagcg gcggcaattg
     1501 cggatgacga cgatgaggaa gaataagtat cgtaagacag cagaatagac aaacgcagat
     1561 atgctgcgga gaatcgaaag attctcgcgc agcataaaaa atgtcaaaag ccgaccgact
     1621 ggtcggacgg aatacaaagt caaagagagg atacagatgt gatttcaaaa tttagtgtaa
     1681 aaagaccgta cactgtcatt gtgggtgtaa tcctggtgat cgttctggga gtggtgtcat
     1741 ttaccagaat gacgactgat ctgctgccgg atatgaacct gccctatgcg ctggtaatta
     1801 caacggatgt gggcgcgagc ccggaggagg ttgagacgga tgtgacggca ccggttgaag
     1861 cctccatggc gacgacatcc agcatcaaaa acgtgagctc cgtatcgtac gacaactatt
     1921 ccatggttat tctggaatac gagcagagcg ccaacatgga ttccattctg attgagatcc
     1981 agcagaagct ggaccagctg gagggaagtt tttcagacag cgtcggaaag ccgatgatta
     2041 tgcagatcga cccggatatg atgccggtca tggtggcgtc ggcggatgtg gaaggcatgg
     2101 atgagtcaga ggtgtcttcc tatgtggaaa atgaattttc accggcactc gagagcgtgg
     2161 agggtgtggc ttcggtaagc gctaccggcg tgcttgagga atccgtgcag gtgaccatgg
     2221 atcaggacaa aatcgatgct ctgaatgata agattcagga aaagatcgag gaacagtttg
     2281 tagatgcgca gaaggaactg gatgatgcat ccgcagagct ggaaaatggc gagaaaaagc
     2341 tggagagtgg caagagcgaa ctggcaaacc aggtcagtgc cgcagccaat cagattgaaa
     2401 atggaaagat tcaggcgtat gtcggcgcat cagaattaaa gacgaatatg accatgatga
     2461 ctgaggcaaa aaagatcatg gagaaggcaa ttccggcact gcagcagctt tataatgatg
     2521 gaattgcagc cagacagcag ctggaagcgc tgaagaa
//
LOCUS       DBMDQJ010000351         2549 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14361_length_2549_cov_1.415033, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000351 DBMDQJ010000000
VERSION     DBMDQJ010000351.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14361_length_2549_cov_1.415033"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            47..886
                     /gene="folD"
                     /locus_tag="ACZO9R_07690"
     CDS             47..886
                     /gene="folD"
                     /locus_tag="ACZO9R_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR0912981.1"
                     /translation="MTQIIDGKKISQEIKDELREQVAEQKKLGKEAVLAVIQVGTDPA
                     SSVYVNNKKKACAYIGIGSLSYELPEETTEEELLALVEKLNGDASVNGILVQLPLPAQ
                     IDADKVIRAIAPEKDVDGFHPENVGKLVIGEPGFVSCTPAGIIQLLKRSGIKMEGKHC
                     VIIGRSNIVGKPMALLMLRENATVTIAHSKTQNLPELCKTADILIVAIGKPQFITADY
                     IKEGAVVIDVGIHRDENNKLCGDVRYDEVAPHTSAITPVPGGVGPMTIAMLMHNCVES
                     MLQ"
     gene            934..>1635
                     /locus_tag="ACZO9R_07695"
     CDS             934..>1635
                     /locus_tag="ACZO9R_07695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR0912982.1"
                     /translation="MKCAKCGAEIKEGCIYCSVCGNAVQIVPDYSVLEDDYLRSLLKE
                     EESAGKEPAPAQAKQQKPKQPQRKKNRRLPFLIGGILLVLLVLAGILIKVAIDHRNAN
                     SYDYQMEQAAMAADAQNDGEALDYYERALYLYPNDITAREKMIEIYEKQKNTDAAMVL
                     LIEIIKLDPANEDAYRDLIAIYDAREDYDSILDLEAEVDAEDLKNKDKILDLFADYMI
                     SAPLITDPEGDVPSGK"
     assembly_gap    1636..1645
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1646..2207
                     /locus_tag="ACZO9R_07700"
     CDS             <1646..2207
                     /locus_tag="ACZO9R_07700"
                     /inference="COORDINATES: protein motif:HMM:NF024686.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR0912983.1"
                     /translation="PKLLGTNFFNLAAAYDYLAINSRMQEKYDEALEVEIASTDGDAI
                     YYTINGDENELDAKTGTRYFVGNPLVFDESGKYTIAAVCRNDKGIYSSVTYADFEIEL
                     APPDFAEVSPDGGRFYEPTLVALTAEDGCSIYYTWDGTDPTTASARYTEPFEVPEGNN
                     VLSVLVVDKKTGLDSGVYRANFIYYP"
BASE COUNT          699 a          540 c          720 g          580 t
ORIGIN      
        1 cggagtcatt accggattat tctaagagcc agaatggagg atacgtatga cacagatcat
       61 tgacggaaaa aagatttcac aggagatcaa ggacgaactg cgcgagcagg ttgcggaaca
      121 gaaaaagctt ggaaaagaag cggtgcttgc agtgatccag gtaggaaccg atccggcttc
      181 cagcgtgtat gtcaataaca aaaagaaagc ctgcgcctat atcggcatcg gttccctgtc
      241 ctatgaactg cctgaggaga cgacggagga agaacttctt gcgcttgtgg agaagctgaa
      301 tggtgatgct tcggtaaacg gaattctggt gcagcttccg cttccggcac agattgacgc
      361 agacaaagtg attcgtgcga tcgcacccga aaaggatgtg gacggattcc acccggaaaa
      421 cgttggaaag cttgtgatcg gggagccggg atttgtctcc tgcacaccgg cgggaatcat
      481 ccagctctta aagcgctccg gaattaagat ggaaggaaaa cattgtgtga taatcggcag
      541 aagcaatatc gtggggaagc cgatggcact tttaatgctg cgcgagaatg ccacggtcac
      601 cattgcacat tccaagacac agaatctgcc ggaactttgc aaaacggcgg atatcctgat
      661 cgttgcaatc ggaaagccgc agtttatcac ggcagattac atcaaagaag gggcggttgt
      721 catcgatgtg ggaattcacc gcgatgagaa caacaagctg tgcggggatg tcagatatga
      781 tgaggttgcg ccgcatacgt cggcgatcac accggttccg ggcggagtag ggcctatgac
      841 cattgcaatg ctgatgcata attgtgtgga gtccatgttg cagtaacaga tgtagaatcc
      901 atatggatga aagagtgaga ggaatcgttt catatgaaat gtgcaaagtg tggggcagag
      961 atcaaagagg gatgtatcta ctgctccgtg tgtggaaatg cggtacagat tgtgccggat
     1021 tacagtgtgt tggaggatga ctatctgcgt tctcttctaa aagaggagga atctgccggg
     1081 aaggaaccgg cgccggcaca ggcaaagcag caaaagccca aacagccgca gaggaagaaa
     1141 aatcgccggc tgccatttct catcggggga attctgctgg tgcttctcgt tctggcggga
     1201 attctgatta aggttgcaat cgatcaccgg aatgccaatt cttacgacta tcagatggaa
     1261 caggcggcca tggcggctga tgcgcagaat gacggggagg cacttgacta ttacgagcgt
     1321 gcgctgtatc tgtatccaaa cgatattacc gcgcgtgaga agatgattga gatctatgaa
     1381 aaacagaaaa ataccgatgc ggcaatggtg ctcctgattg agatcataaa gcttgatcct
     1441 gccaatgagg acgcatacag agatctgatc gctatctatg atgcgaggga agattacgac
     1501 agcattctgg atctggaagc ggaggtcgat gccgaggatc ttaagaataa ggataagatt
     1561 ctggatttgt ttgcggatta catgatatct gcaccgctga ttacggatcc tgagggagat
     1621 gtgccgtccg gaaagnnnnn nnnnngccga agcttttagg tacaaatttt ttcaaccttg
     1681 ccgcagccta tgactatctg gcgatcaata gccgcatgca ggaaaagtat gatgaggcgc
     1741 tcgaggtaga gattgcgtcg acagacgggg atgccatcta ctatacgatc aatggagatg
     1801 agaacgaact ggatgccaag accggaacga ggtattttgt cggaaacccg ctcgtctttg
     1861 atgagagtgg caaatatacc atcgcggcgg tctgccggaa cgataaggga atctacagtt
     1921 ccgtgacgta tgccgatttt gagatcgaac ttgcaccacc ggattttgca gaggtgtcgc
     1981 cggacggagg ccgtttttat gagccgacac tcgtggcgct gaccgcagag gatggatgca
     2041 gcatttacta tacctgggac ggaaccgatc ctaccacggc gtctgcgcgc tacacggagc
     2101 cgtttgaggt gccggagggc aacaatgtcc tgtccgtact tgtggtggac aaaaagacgg
     2161 gattggacag cggtgtttac cgtgccaatt ttatctatta cccgtaaaaa ggaattgccg
     2221 caataagcat aacctctttg gggtgataag taatattata agaaacatac ttggagattt
     2281 catgtggaaa tgcagctgga aattcgtttg agaaaaatgt gagtatttct tccagccgaa
     2341 tgtttgcatc ggattccgtg tctcttccac gtttgaacac tcccggagtg tgtccgccaa
     2401 atcagagaca atgagcaaac tttctagtgg cggctaatgc cgctgcttca cagacactat
     2461 aaaatcgcgc gaaccgaaac tcgctgccat ctgctattga aaataaggca gatggcaact
     2521 cagacatcgg cttcgcgcga ttatgtttt
//
LOCUS       DBMDQJ010000352         2548 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14366_length_2548_cov_1.749489, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000352 DBMDQJ010000000
VERSION     DBMDQJ010000352.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14366_length_2548_cov_1.749489"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            360..1442
                     /gene="mtnA"
                     /locus_tag="ACZO9R_07705"
     CDS             360..1442
                     /gene="mtnA"
                     /locus_tag="ACZO9R_07705"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR0912984.1"
                     /translation="MGYDTVRLDDENNAVVIIDQTKLPGKIEIISLHTAQEIWNAIYL
                     LQVRGAPAIGVAAAYGIYVLAKRMDTEDYDTFYREFVRQKEYLDSSRPTAVNLSWALN
                     RMQRVVEAHSGETVAQIKEALHRESVAIQEEDIWVCRMIGEHGLTLVKPGDGILTHCN
                     AGQLATSKYGTATAPIYLGEERGYHFRVFADETRPLLQGARLTAFELQSSGVDVTLIC
                     DNMSATVMKNGWVNAVFVGCDRVAANGDAANKIGTSVVAAVAKYYGVPVYICAPTSTI
                     DLNTPTGAEIKIEQRPAEEVTEMWYKERMAPEGIKVFNPAFDVTDHELIAGIVTEYGV
                     ARAPYTESLAAIFAEKEKRAREKKEA"
     gene            1478..2332
                     /locus_tag="ACZO9R_07710"
     CDS             1478..2332
                     /locus_tag="ACZO9R_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HIR0912985.1"
                     /translation="MPENSNCSGASSCSKESCEGCPSNAKNKAGGGIAKEPMNAASNV
                     KKVIGVVSGKGGVGKSFVTASLAAAMNRAGYKVGIMDADITGPSIPKMFGIHGQVYGT
                     DDGMVPMETENGIKIMSINLLLDDEEAPVIWRGPVIAGVVKQFWNETVWGDVDYLFVD
                     MPPGTGDVPLTVFQSLPVDGIVIVTSPQELVQMIVKKAYNMAEMMHVPVLGLVENFSY
                     LKCPDCGKEIKLFGESHIDDVAKELGIPVFGKLPLDPAFAEQADKGAFATMENAYLDA
                     GVEKLKTL"
BASE COUNT          679 a          533 c          774 g          562 t
ORIGIN      
        1 gggacaccag aaagtccgcc cattgtctcc ggtgtgtcgg acatgcaccc gtcggatttt
       61 ccaaacgtgg aagagacacg gaatccgatg caaacattca gcaggaagaa atgcacacat
      121 ttttctccaa cgaatttcca tttgtatttc catctgaaat cccacggtgt ggtcttctca
      181 tattattact tatcacccaa acgatctatg cttattgcgg caattccttt tttgtctgca
      241 aaaaagaaca cgagaaagcg ggcgtcttct tggaaacatc gacaaaagag agtataatag
      301 tctgtgatga ataagaataa aggggtgaaa gtgtgggaga acagcagagc aggaatatta
      361 tgggttatga caccgtgcgg ctggatgatg agaacaatgc agtggtgatc atcgaccaga
      421 cgaagctgcc gggaaaaata gagatcattt cgcttcacac ggcgcaggaa atctggaacg
      481 cgatctatct gcttcaggtg cgcggagcac cggcgatcgg agtggcggcg gcatatggga
      541 tctatgtgct ggcaaaacgg atggatacgg aggattacga tacattttac cgtgagtttg
      601 tacggcagaa ggagtatctg gattcttcgc gcccgacagc cgtgaactta agctgggcgt
      661 taaaccggat gcagcgtgtt gtggaggcac acagtgggga gactgtagcg cagatcaagg
      721 aggcactgca ccgggagtct gtggcgatcc aggaggaaga tatctgggtg tgccggatga
      781 tcggagagca tggtctgact ctggtaaagc cgggggatgg cattctgacg cactgcaatg
      841 cgggacagct cgccacaagc aaatatggca ccgcgacggc gccgatctat ctgggagagg
      901 aacgcggtta tcattttcgt gtatttgcgg atgagaccag acccctgttg cagggcgcca
      961 gactgacagc atttgaactg cagtcttccg gggtggatgt cacattgatc tgcgacaata
     1021 tgtcggcgac agtcatgaag aacggctggg ttaatgcggt atttgtcggc tgtgaccgtg
     1081 tggcggcgaa cggagatgcg gcgaataaga tcggtacttc tgtggtggca gcggtggcaa
     1141 agtattacgg tgtgccggtg tatatctgcg cgccgacgtc gacgatcgac ttaaatacgc
     1201 cgaccggagc tgagatcaag atcgagcagc gtccggcgga ggaagtcacg gaaatgtggt
     1261 acaaggagcg catggcaccg gagggcatca aggtcttcaa tccggcattt gatgtgacgg
     1321 atcatgagct gattgcaggc atcgttaccg aatacggtgt ggcgagagcg ccttacacgg
     1381 agtcgctggc ggcgattttt gcagaaaagg aaaagcgtgc cagagagaaa aaagaagcct
     1441 gatagagaag atgtaagcga tttaaggagg aaagaatatg ccagagaaca gtaattgcag
     1501 cggagcatcc agctgctcaa aggagagctg cgagggatgt ccgagcaacg cgaagaataa
     1561 agcaggcggc ggaattgcca aggagccgat gaatgcggca tccaacgtta agaaagtgat
     1621 cggtgttgtc agcggaaaag gcggcgtcgg caaatcattt gtcacagcat cactcgctgc
     1681 agcgatgaac cgcgccggat acaaggtagg catcatggat gcggatatta ccggtccgtc
     1741 gattcctaag atgttcggaa ttcatggaca ggtatacgga acggacgacg gaatggttcc
     1801 gatggagacg gagaacggca tcaagatcat gtcgatcaac ctgctgttgg atgacgagga
     1861 ggcacctgtc atctggagag gtccggtgat tgccggcgtg gtaaaacagt tctggaacga
     1921 gacagtctgg ggagacgtgg actatctgtt cgtggatatg ccgcctggaa ccggagatgt
     1981 accgcttacg gtgttccagt cgcttccggt agacggaatc gtgattgtga cgtccccgca
     2041 ggaactcgtg cagatgattg tgaaaaaggc gtacaacatg gcggagatga tgcatgtgcc
     2101 ggtgctcggt ctggtggaga atttcagcta cctgaaatgt ccggactgcg gaaaagagat
     2161 caagctgttt ggcgagagtc atatcgacga tgtcgcaaaa gagctcggga tcccggtatt
     2221 cggaaagctt ccgcttgatc cggcgtttgc ggagcaggca gacaagggag cgtttgcaac
     2281 gatggagaac gcttatctgg atgcgggcgt agagaagtta aagacacttt aatgtgacag
     2341 gaacgggaaa gtgttaccac ttacccggag aaaatgacct cttggtgtaa aactgtttac
     2401 accgggaggt tcttttttta tactggaacc atcaaaggag ttacagagat gaaaataaaa
     2461 attgagattt gtataactaa aatctcaact ttccttattt tatgcgactt ggcggtatgc
     2521 cttgccactc tatatgtatc cttttccc
//
LOCUS       DBMDQJ010000353         2545 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14390_length_2545_cov_1.407119, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000353 DBMDQJ010000000
VERSION     DBMDQJ010000353.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14390_length_2545_cov_1.407119"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="ACZO9R_07715"
     CDS             complement(<1..661)
                     /locus_tag="ACZO9R_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HIR0912986.1"
                     /translation="MALGLAACGTDPTTVDYFGMTYDELQSSMEQEVSALVGLTDDNR
                     TYIQSYGSDTAMKLVESWDEATSDVGAYQGLGEFNITKAQKTLTVDQYVEYQNREVIV
                     SYIYTYNYETEQPELTDASADKVYTLGEKMEKAALNTLMGMGTVFVVLILISLIIYCF
                     RFISVIQNKAAGKGKKNEAEAGAVVEQIGQREEQLTDDLELVAVISAAVAASTGASAD
                     SF"
     gene            complement(714..2150)
                     /locus_tag="ACZO9R_07720"
     CDS             complement(714..2150)
                     /locus_tag="ACZO9R_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR0912987.1"
                     /translation="MNSSNDSLAMQRITRLVDENSFIEIGSLVTARSTDFNLAATDTP
                     SDGVITGHGLIDGNLVFIYSQDASVLNGTIGEMHAKKIAAVYDMAMKMGAPVIGFIDC
                     AGVRLQESVDALDGFGLIYAKEVAASGVVPQISAVFGSCGGGLAVVPALSDFAFIEES
                     KGKMFVNSPNAIDGNRIEKCDTSSAAFQADENGCIDATGTEDEILTDIRELVGMLPLN
                     NEGDVYTDECVDDLNRACESMDAMKGDPRYLLSEISDSHTFFETKAGYARDMVTGFIK
                     LNGMTVGAVANCTTVYDEEGKESEKFDNVLSAKGCEKAAEFVSFCDAFEIPVLTLTNV
                     KGYKACKCSEKRLAKALAHLTSAFAGATCPKVNLITGEAYGTAYVAMNSKSIGADFVY
                     AWPDAKVGMMDADLAVKIMYADASADELAEKAKEYDALQGSVMTAARRGYVDLIVDPA
                     DTRKYLVDAFELLYTKCAYTPDKKHGTK"
     gene            complement(2336..>2545)
                     /locus_tag="ACZO9R_07725"
     CDS             complement(2336..>2545)
                     /locus_tag="ACZO9R_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR0912988.1"
                     /translation="APLSSEVTLKYEIADRLYAPVKSGDYIGKIRYMLGEETLAERCV
                     VAGETIGKIDYPWCLGQVLRLLWIE"
BASE COUNT          594 a          716 c          519 g          716 t
ORIGIN      
        1 cgaaagaatc cgcggatgca ccggtgcttg ccgcaaccgc tgcggaaata actgccacaa
       61 gttcaagatc atcggtaagc tgctcttcac gctgtccgat ctgctctacc acagcgcctg
      121 cctctgcctc gttcttcttg cccttgccgg ccgccttgtt ctgtattaca gagataaagc
      181 ggaaacagta aataataagg ctgatcagga tcagaaccac gaatacggtt cccattccca
      241 tcagggtatt taacgcagcc ttctccatct tctcacccag cgtatatacc ttgtccgcgg
      301 atgcatcagt aagctccggc tgctcggtct cgtaattgta tgtatagatg taagaaacga
      361 tgacttcacg attctgatac tctacatact gatctactgt taaggttttc tgtgcctttg
      421 tgatattgaa ttctccaagt ccctgatatg cgccgacatc acttgtcgcc tcatcccagc
      481 tctcgacaag tttcattgcc gtatcgctgc cgtaggactg gatataggtt ctgttgtcgt
      541 ccgtaagacc aaccagcgcg gaaacttcct gttccatgct gctctgcagt tcatcgtaag
      601 tcattccgaa atagtcaacc gttgtcggat ctgttccgca tgctgcaagt cccagagcca
      661 taaagcaaag acaaagtata agagataatt ttttcttcat aaacttcacc tttctatttt
      721 gttccatgtt tcttgtccgg tgtatatgca cactttgtat ataacagctc aaacgcatct
      781 accagatact ttctcgtatc tgccggatca acgatcagat ccacatagcc tcttctggct
      841 gctgtcataa cgctgccctg cagtgcatca tattcctttg ccttctctgc aagctcatcc
      901 gcagatgcat ccgcgtacat gatctttact gcaagatccg catccatcat gccgaccttt
      961 gcgtccggcc atgcgtatac gaaatccgca ccgatagact tggaattcat agcaacataa
     1021 gcggtaccgt atgcctctcc cgtgatcagg ttcaccttcg gacatgtagc tcccgcaaat
     1081 gcggaggtca gatgagcaag tgcctttgca agacgcttct cagagcactt gcatgccttg
     1141 tagcccttga cattcgtcag agtcagtacc gggatctcga atgcgtcgca gaaagatacg
     1201 aactctgctg ccttctcaca tccctttgca gaaagaacat tgtcaaactt ctcgctctct
     1261 ttgccctctt cgtcgtatac cgttgtgcag tttgcaactg cgccgaccgt cataccgttt
     1321 aacttgataa atccggtcac catatctctg gcatagcctg cctttgtctc gaagaatgta
     1381 tggctgtccg aaatctcact gagaagatat ctcggatcgc ccttcatggc atccatgctc
     1441 tcgcatgcac ggttcaggtc atccacacac tcgtctgtgt agacatctcc ctcgttgttg
     1501 agcggaagca tgccgaccag ctcacggatg tctgtaagga tctcatcctc tgttcccgtc
     1561 gcatcgatgc agccgttctc atctgcctgg aatgccgcag atgatgtgtc gcacttctcg
     1621 atgcggtttc cgtcaattgc gttcggagaa ttcacaaaca tctttccttt ggattcttcg
     1681 ataaaagcaa aatcagaaag tgccggtacc actgccagtc caccgccgca gctgccgaat
     1741 acggctgaaa tctgcggaac cacgccggat gctgcaactt cttttgcata gatcagtccg
     1801 aagccgtcaa gcgcatctac agactcctgt aaacggactc ccgcgcaatc gataaagccg
     1861 atcaccggtg ctcccatctt catcgccatg tcatacactg cagcgatctt cttcgcatgc
     1921 atctcgccga ttgttccgtt taaaacagaa gcgtcctggc tgtaaatgaa caccagattg
     1981 ccgtcaatca ggccgtgtcc tgtgatgact ccgtcggaag gagtatccgt tgctgcaaga
     2041 ttaaaatctg tgcttcttgc ggttacgaga gaaccgatct caatgaaact gttctcgtcg
     2101 actaacctgg tgatgcgctg catagccagg ctatcattgc tactattcat tctttttgcc
     2161 ctccatctat aaattgttgt ccacatccat ttgggatgcg gcttcatagt gaatgatgta
     2221 aatccatgcg tctttgtata aaaattcaac aaattacgct atgttaaatt ataattgctt
     2281 ttctgcaata tttcaagtaa attgttaatt ttttgacgct ttttgtttgt gttttttatt
     2341 cgatccagag aagccgaagc acctgcccca gacaccacgg ataatcgatc tttccgattg
     2401 tctccccggc taccacacat cgctccgcga gcgtctcctc ccccagcata taacgaatct
     2461 tcccaatgta atcgccgctc ttcaccggtg catacagccg gtctgcaatc tcatacttaa
     2521 gcgtaacctc actgcttagc ggcgc
//
LOCUS       DBMDQJ010000354         2544 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14398_length_2544_cov_1.819894, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000354 DBMDQJ010000000
VERSION     DBMDQJ010000354.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2544)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2544)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2544
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14398_length_2544_cov_1.819894"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(306..1655)
                     /locus_tag="ACZO9R_07730"
     CDS             complement(306..1655)
                     /locus_tag="ACZO9R_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0912989.1"
                     /translation="MKDLTKGKPSTLILAFALPIFLGNLLQLTYSVTDTRIVGSFLGE
                     DALAAVGATTTLSNLIIGFLLGLANGFAIITAQRFGAKDIRSVKKSFAASLVLGTVIS
                     VVLTVLGLVFLQPILRFLNVPEHLIPVAGPYIFIIIAGLLATFLYDACAAALRALGDT
                     VTPLVILAVSVALNIAGDLLFVLVLKAGVRGAAAATVLAQILAFVICWLYMLRRYEML
                     RLVREDFRDADTAMMKNMLGSGLSMGFMSSLVNIGSLTLQTAINKLGQDIIVAHTAAR
                     KISEIFMTMFSVFGQTMATYCGQNLGAGRIDRVKKGIRLGIFYTCIWCTLSAVASYTI
                     GRWLVYLVTGSTNEVVILNATNYLKFDTLFYYVTAVICVLRNAMQGLGDHITPLISSS
                     LEMVGKIVIAATLVPMLGYTGVIVAEPLVWFIMVIPLVVQIFRMPVLREENRGNQGK"
     gene            complement(1652..2413)
                     /locus_tag="ACZO9R_07735"
     CDS             complement(1652..2413)
                     /locus_tag="ACZO9R_07735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR0912990.1"
                     /translation="MEQCRENEAAIVTGASSGIGAAISARLCKMGYEVFGIGRSFGSS
                     FAEQFPDAADNPLFHAVVCDLLDTEKMLKLVRGIAAEATVTLLVNNAGSAYYGLHEEL
                     SPKKIQEMVRTDLEVPMILSQQLLRGFKKNKGCIVNIASVTAQQSNPHGCAYGAVKAG
                     LASFSRSLFDEARKYGVRVAVVSPDMTKTELYRNADFTVGEETESYLLPQDVADAVAY
                     IVGQREGVAVSEVTLRPQYHRIRRKPVAKKERPVE"
BASE COUNT          570 a          791 c          572 g          611 t
ORIGIN      
        1 tcaaataagt tctctccaaa aatactaggt gttaacataa gtcatctctc cttttctact
       61 tcgtacctgc cttcggcaga tacactcatg ctgtttcctt gtttctttgg aaccgggatg
      121 ttctgggaac tcatagaact catttcctgt tctccttttg ttcctctcct ttgttctgac
      181 tatgttataa caccgtttat tagcactgtc aagtgttgag tgctaatttt ctgtgaactt
      241 tttgtgaaat cctgtaatta cttgatttta tggcatttca agcgttgtta tcgtccaact
      301 acgcatcatt ttccttgatt tcccctgttt tcttcccgaa gtaccggcat ccggaagatc
      361 tgcacgacga gcggaattac catgataaac cacacgagcg gctccgccac aataacgccg
      421 gtgtagccta acatcggcac aagcgtggcg gcgatcacga ttttcccgac catctcaagc
      481 gaactcgaaa tgagcggcgt gatatgatcg cccagtccct gcatggcgtt tcggagcacg
      541 cagatcaccg ccgtcacata gtagaacagc gtgtcgaatt tcaggtagtt ggtcgcattt
      601 aagatcacaa cttcattcgt gcttcccgtc acaagatata ccagccatct accaatcgtg
      661 tagctcgcca ccgcggaaag cgtacaccag atgcaggtgt agaaaatgcc gagccggatg
      721 ccttttttaa cccggtcgat cctgcctgcg cccagattct gtccgcagta agtcgccatc
      781 gtctgtccga agacactgaa catcgtcata aaaatctcgg aaattttccg tgccgccgtg
      841 tgcgccacga tgatatcctg ccccagctta ttgatcgccg tctggagtgt cagcgagccg
      901 atattgacca gcgagctcat aaagcccatt gacagccccg atccgagcat gtttttcatc
      961 atcgccgtat ccgcatcccg gaaatcctcc cgcacaaggc ggagcatttc ataccgtctc
     1021 aacatgtaga gccagcagat cacaaacgcc aggatctgtg ccagaaccgt ggcggcggca
     1081 gctccccgca ctcccgcttt taacaccagc acgaacagaa gatctcccgc gatatttaac
     1141 gccaccgaca ctgccagaat cacgagcggc gtgacggtat ccccgagcgc ccgaagcgcc
     1201 gcggcacacg cgtcatagag aaatgtcgca agaagtcccg ctatgatgat aaatatgtaa
     1261 ggtcccgcca ccggaatcag atgttccggc acatttaaaa accgcagaat cggctgtaaa
     1321 aacaccagtc ccagcaccgt cagcacgacc gagatcaccg ttcctagcac cagggaagcg
     1381 gcaaacgatt ttttcacgct ccggatatcc tttgcgccga atcgctgcgc cgtgatgatg
     1441 gcaaacccgt tcgccagtcc gagcaaaaag ccgatgatga gattgctgag cgtcgtcgtt
     1501 gcaccgactg cggcgagcgc atcctccccg agaaaagagc cgacaattct cgtgtcggtc
     1561 acgctgtagg tcaactgcag cagattcccc agaaatatcg gcagtgcaaa cgcaagaatc
     1621 agcgttgacg gttttccttt tgtaagatct ttcattcgac cggtctctcc tttttcgcta
     1681 ccggcttgcg ccggattctg tgatactgcg ggcggagcgt cacctccgac accgccacgc
     1741 cctctctctg ccccacgata tacgccaccg cgtccgcgac atcctgcggc agaagatagc
     1801 tctccgtctc ctcccccacc gtaaagtctg cattgcggta caattccgtc ttcgtcatat
     1861 ccggggatac caccgccacc cgcacaccgt attttcgcgc ctcgtcaaag aggctgcgcg
     1921 aaaagctcgc aagtcctgcc tttaccgcgc cgtaggcaca gccgtgcgga ttcgactgct
     1981 gcgccgtcac ggatgcgata ttgacgatgc agcccttgtt tttcttaaac ccgcgcagca
     2041 gctgctggct taaaatcatc ggcacttcca gatctgtgcg caccatctcc tgaatcttct
     2101 tcgggcttag ttcctcgtgc agtccgtagt acgcgctgcc ggcattgttg acgagcagcg
     2161 ttaccgtagc ttccgccgca atcccccgca ccagcttcaa cattttttcc gtgtccagaa
     2221 gatcgcagac caccgcatga aacagcggat tatctgcagc gtccggaaac tgttccgcga
     2281 aactgctgcc gaacgagcgt ccgatgccaa acacttcata tcccattttg cacagtctgg
     2341 cactgatcgc cgccccgatc ccggaggacg cccccgtgac aatcgccgcc tcgttttctc
     2401 tacactgctc catgtattct ctccttattc cttccaccgg aaaatccgct ctgccggaag
     2461 ataagcggca agtttttccg tcatatactg ctccatctcc tccatgagcg ccgtcgggta
     2521 atggtacacc ccgccgtcat tctg
//
LOCUS       DBMDQJ010000355         2540 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14430_length_2540_cov_1.216892, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000355 DBMDQJ010000000
VERSION     DBMDQJ010000355.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14430_length_2540_cov_1.216892"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="ACZO9R_07740"
     CDS             <1..1518
                     /locus_tag="ACZO9R_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012464.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR0912991.1"
                     /translation="VSTEEEEQQSSYEAQCTYYTDKIMSNPEWNMAGIFADEGITGTS
                     TKKRENFNRMIRKCRQKKIDLILTKSISRFARNTVDSLKYIRALKELGIGIIFEKENI
                     NTLEMDTELIITFMSAFAQSESESISANVKWGKRHAMKEGKASINFNKIFGYYLDNKG
                     DPKVDQDKAVIIRGIYDYYIQGASLRMIKQDLEARGVPNPAGGENWGVEQIRNILSNE
                     KYCGDVLLQKTFTQDCISKKVVKNTGQLPMYLIQNNHPAIVSRGVYQAVQAEKARRSA
                     SASPSTKTSSTGRTCYASKYALSERLVCGECGTLYRRCTWKRNGKTKIVWRCVSRLDY
                     GTKYCHDSPTMEEGALQQAIMTAINSVMSPKGKLIQQIADAMVEETVKPPDSVMTLGE
                     INRSLEKLEAEFDALLHQSDSAEKNADRFKKIANEMASLKKQREKLSAQLRNNQATQE
                     RVRTIKGALDHANHYMIEWDEEMIRQLVHTVKVISADRIRVYLHDGTEIDQAVSP"
     gene            complement(1655..1897)
                     /locus_tag="ACZO9R_07745"
     CDS             complement(1655..1897)
                     /locus_tag="ACZO9R_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024835.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0912992.1"
                     /translation="MNAKEAVAARILELCNERGIAINALAGSAGVSPSTVYSMLNAKS
                     QNPGVVSLQKLCDGLGITLREFFDDDLFDNIEQEIK"
     gene            2036..2524
                     /locus_tag="ACZO9R_07750"
     CDS             2036..2524
                     /locus_tag="ACZO9R_07750"
                     /inference="COORDINATES: protein motif:HMM:NF018601.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLOG family protein"
                     /protein_id="HIR0912993.1"
                     /translation="MDLRAKTCCFTGHRSLSGAEKLKAAVRLRRVIEEQIKAGIVFYG
                     AGGALGFDTLAAQTVLDMKKEYPQLRLILVLPCEGQTRGWRSEDIAVYEDIKRRSDKV
                     VYVSREYTPDCMHRRNRHLVDHSGTCICYLTRSAGGTAYTVDYARKKGLRIINLAEKC
                     HI"
BASE COUNT          749 a          529 c          691 g          571 t
ORIGIN      
        1 gtctctacgg aggaagaaga acagcagtcc agctatgagg cccagtgtac ctactacacg
       61 gataaaatta tgagcaatcc ggaatggaac atggccggta tttttgccga cgagggcatt
      121 actggaactt ctacgaagaa gcgggagaat tttaaccgta tgatccggaa atgccgtcag
      181 aagaaaatcg atttgatcct gacaaaatcc atatcccggt ttgcccgaaa cacggtagac
      241 agcttgaagt atattcgtgc gctgaaggaa ctggggatag gcattatctt tgagaaagag
      301 aatattaata ctttggaaat ggacacggaa ttaatcatca cttttatgag cgccttcgcc
      361 cagtcagaaa gtgaatccat cagcgcgaat gtaaagtggg ggaaacggca tgctatgaaa
      421 gaaggaaagg cttccattaa ttttaataag atattcggct attatctgga caataaggga
      481 gatcctaaag tggatcaaga taaggcggtg atcatccgcg gtatctatga ctattacata
      541 cagggagcca gcctgcgcat gattaagcag gatctggaag ccagaggagt tcccaatccg
      601 gctggcggcg agaactgggg tgtcgaacag atccggaata tcctgagcaa tgagaaatac
      661 tgcggagatg tactgctgca gaaaacattt acgcaggatt gtatcagcaa gaaagttgtt
      721 aagaataccg gacagcttcc gatgtacctc atccagaata atcatccggc gatcgtgagc
      781 aggggagtgt atcaggcagt ccaggctgag aaggcaagaa ggtcagcatc agccagtccc
      841 tcgacaaaga cctcttcaac gggccgtacc tgttatgcca gtaaatatgc gctgtcagaa
      901 cggctcgtgt gcggtgaatg cggaaccttg tatcggaggt gtacatggaa acgaaatggc
      961 aaaacgaaga tcgtctggcg ctgtgtaagc cgactggatt acggcacaaa atattgccat
     1021 gattcaccca caatggaaga aggagcatta cagcaagcca ttatgacagc aattaattcg
     1081 gtcatgagcc cgaaaggaaa actgatccag cagatagcgg atgccatggt tgaggaaaca
     1141 gttaagcctc ctgattccgt aatgacgctg ggagaaatca atcgaagcct tgagaaactg
     1201 gaagcggaat ttgatgccct gctccatcag tctgacagtg cggaaaagaa tgcggacagg
     1261 tttaagaaga tcgccaatga gatggcatct ctgaagaaac agcgggagaa gctatccgcc
     1321 cagctccgga ataaccaagc tacacaggag cgtgttcgaa ctataaaagg agcgctggat
     1381 catgcaaatc attacatgat agagtgggac gaagaaatga tccggcagct tgtacataca
     1441 gtaaaagtga tttctgccga tcgtatcagg gtgtacctgc atgacggcac ggagattgat
     1501 caagcggtca gcccataaca gatagcggaa tatgggagaa tgttcccaaa tcgtacagcg
     1561 ggttcaacgc aggagacgag tctggattgt gatcttcatt cggaccatag atatcagaag
     1621 cgccggtatc tgaaagaacc ggcgctcctg ctgcttattt aatctcctgc tcaatgttgt
     1681 cgaagagatc atcatcgaaa aattcccgaa gggtaattcc caacccatca cagagcttct
     1741 gcagggaaac cacgccggga ttctgactct ttgcattgag catgctgtaa actgtggagg
     1801 gcgagactcc cgcacttccg gcaagggcat tgatggcgat tcctctttca ttgcacagct
     1861 ccagaattct tgctgcgact gcttctttgg cgttcataaa tcatcatcct tctgtacgat
     1921 atatcgctga ctgtattcta taaaaagttg ggatatatcg agtgggatat atcgcaatta
     1981 tgcggaacct gtggtaaaat atgcgatata tcgtagaaca aggaggaaga tatttatgga
     2041 tttaagagcg aaaacctgct gctttacggg gcacaggagc ctgagcggcg cagaaaagtt
     2101 gaaagcggct gttcgtttgc gtagagtgat tgaggaacag atcaaggctg ggattgtgtt
     2161 ttatggagcc ggaggcgcgc tggggtttga tacattggct gcgcagacgg ttctggacat
     2221 gaaaaaagaa taccctcagc tgcgcctgat tctggtgctg ccctgtgagg gtcagaccag
     2281 aggatggcgc agtgaggata ttgcagttta tgaggatatc aagcgcagaa gcgataaggt
     2341 tgtgtatgtg tcccgggaat atacaccgga ctgcatgcac aggaggaatc gccatctggt
     2401 ggatcacagc ggaacctgta tctgctacct gacaagaagc gccggaggaa cggcatatac
     2461 cgtagattac gcgcgaaaaa aggggctgcg catcattaac ctagcggaga aatgtcatat
     2521 ctgagatggc attggcagct
//
LOCUS       DBMDQJ010000356         2539 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14437_length_2539_cov_1.692781, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000356 DBMDQJ010000000
VERSION     DBMDQJ010000356.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14437_length_2539_cov_1.692781"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1121
                     /locus_tag="ACZO9R_07755"
     CDS             <1..1121
                     /locus_tag="ACZO9R_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HIR0912994.1"
                     /translation="NHLHEQKMPGKKAMLVISNGKSTRASGALDRTMKELSLAGVEVV
                     LFDKVGANPVKEIVEEGGHFAKKEGCDFIVALGGGSVMDAAKVMAMYAQQPGDLWDYV
                     AGATGKQQPLVNPVLPVIAITTTAGTGSEVDQWGVVTNPATNEKIGCGGMDELFPVIA
                     VVDPELMLTVPPKFTAYQGFDALFHSVEGYLSVVSSPMSEMVQLAAIENIGTYLPRAV
                     KDGSDLEAREHVAFANTMSGYSMVVGSCTSEHSMEHAMSAFHPALPHGAGLIMISVEY
                     YRHFIEKHCCDERFVRMAQALGMKDAKAPEDFITALKKLQEDCGVSDLKMSDYGIEKE
                     ESMVLARNAKATMGGLFACDPVPMTDEECTAIYEKSYR"
     gene            1181..1660
                     /locus_tag="ACZO9R_07760"
     CDS             1181..1660
                     /locus_tag="ACZO9R_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HIR0912995.1"
                     /translation="MSQKSLVAYFSCSGVTKKTAELLSDMAGADLFEIRPEVPYTKAD
                     LDWMDKKSRSTVEMNDPSYRPAIADKVEHMEQYDTVYVGFPIWWYVAPTIINTFLESY
                     DFSGKTVIPFATSGGSGMGKTEAVLHALCPDSVNWKSGKMLNGYDRAQMEAWVRTLA"
     gene            1780..>2539
                     /locus_tag="ACZO9R_07765"
     CDS             1780..>2539
                     /locus_tag="ACZO9R_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR0912996.1"
                     /translation="MEKVRYMISDAANIVHVESHVLRYWEDELELNIPRNEMGHRYYT
                     KENIAEFQRIKELKEQGYQLKAIRMIVHNGPIEGLTAEAPIPVGQAPLSAGAAAAIPQ
                     PVQPVAAQPAAVAQPVQQSVQPVQSAAPQQLPVKTTAESPQSILNNTDKMAQFTELMT
                     EIVGKALAANNEELGQSISEEVGKQVIKEMNYLMREREEAEEDRFRRLDAAIRGNIRK
                     KDEQKEKKLWKKKKLQSSIMRMIRMFINCIHKHRK"
BASE COUNT          708 a          546 c          778 g          507 t
ORIGIN      
        1 taaatcactt acacgagcag aagatgcctg gaaaaaaggc aatgcttgtc atctcaaatg
       61 ggaaatcgac cagagcgagc ggcgcacttg accgtacgat gaaagagctt tccctcgcag
      121 gtgtggaggt tgtgctgttt gataaggtcg gagcgaatcc ggtcaaggag atcgtcgagg
      181 aaggaggaca ttttgccaag aaggaaggct gcgatttcat tgtcgcactg ggaggcggct
      241 ccgttatgga cgcggcgaaa gtcatggcaa tgtatgcgca gcagccggga gatctctggg
      301 attatgtggc aggagcaacc ggaaagcagc agccgttagt gaatccggta cttccggtca
      361 tcgcgatcac gacaacggca ggaaccggat ccgaggtgga tcagtggggc gttgtgacca
      421 atccggcaac gaatgagaag atcggctgcg gcggcatgga tgagctgttt ccggtgattg
      481 cagtggtaga tccggagctg atgctgaccg taccgccgaa attcacggca taccaggggt
      541 ttgatgcgct gttccacagc gtggaaggat acctctcggt tgtgagcagc ccgatgtctg
      601 agatggtcca gcttgcggcg atcgagaata tcggaaccta cctgccgcgc gcggtaaagg
      661 acggcagcga tctggaggcc agggagcatg tggcatttgc caatacgatg tccggctact
      721 cgatggtggt cggttcctgc acaagcgaac attcgatgga acatgcgatg agcgcatttc
      781 atccggcgct gccgcacggt gcgggactga ttatgatttc ggtggagtat tacagacact
      841 ttatcgaaaa acactgctgt gatgagcggt ttgtccgcat ggcgcaggca ctcggcatga
      901 aagatgccaa ggcgccggag gattttatca cagcgttaaa gaaactgcag gaagactgcg
      961 gcgtatcaga cttaaagatg tccgattacg gcatcgaaaa agaggagagc atggttctcg
     1021 ccaggaatgc aaaggcgacg atgggtggac tgtttgcctg tgatccggtg ccgatgacag
     1081 acgaagagtg cacggcgatt tatgagaagt cttaccgcta gagaaggttg ttttaaacat
     1141 tttaatttat agaaaaaaga gttaagaatg gaggattatg atgagtcaga agagtcttgt
     1201 agcttatttt tcatgcagcg gcgtcacaaa gaagacggca gagctgcttt cggatatggc
     1261 aggagcggat ctgtttgaga ttcgtcccga ggtgccttat accaaggcgg acctggactg
     1321 gatggacaaa aagagccgca gcacggtgga gatgaacgat ccgtcctacc gtccggcgat
     1381 tgcggacaag gtagagcata tggagcagta cgacaccgtc tatgtgggat tcccgatctg
     1441 gtggtacgtt gcgccgacga tcatcaacac gttcctggag agctatgatt tttccggaaa
     1501 gacggtgatc ccgtttgcaa catctggcgg aagcggtatg ggaaaaacag aagcagtgct
     1561 gcatgcactc tgcccggaca gcgtgaactg gaagagcggt aagatgttga acggatatga
     1621 cagagcgcag atggaggcgt gggtcagaac actcgcctag ttctgtgaaa aataagaaag
     1681 accggcgatt tgtgtcgcaa taagacgagc ggtttttgtt gtacagaaga agcggtacac
     1741 ctataatata agttaaggaa ctacaatata ggagggtgta tggaaaaggt acgttatatg
     1801 atttccgatg cggcaaatat cgtccacgtc gaatcacatg tgttacgtta ctgggaagat
     1861 gaactggaac tgaatatccc gcgcaatgag atgggacacc ggtattatac gaaggaaaac
     1921 attgcggaat ttcagagaat caaggaacta aaggaacagg gctatcagtt gaaggcgata
     1981 cgcatgattg tacataacgg accgatcgag gggctgacgg cagaagcacc gattcccgtg
     2041 ggacaggcac cgctttctgc gggagcggcc gctgcaatcc cgcagccggt acaaccggtc
     2101 gcggcacagc cggcagcagt ggcacagccg gtgcagcaat ccgtgcagcc ggtgcagtcc
     2161 gctgcacccc agcagcttcc ggtaaaaacg accgccgaga gcccgcagag tattctaaac
     2221 aatacggata agatggcaca gttcacagaa ctgatgaccg agattgtggg aaaagcgctt
     2281 gccgccaaca atgaagaact ggggcagagc atcagcgagg aggtaggaaa acaggtgatc
     2341 aaggagatga attacctcat gcgggagcgg gaggaagcag aggaggaccg cttccgcagg
     2401 ctggatgctg cgatccgtgg aaatatccgc aaaaaggacg aacagaaaga aaagaaactc
     2461 tggaaaaaga aaaagctaca atcgtctata atgcggatga tcagaatgtt cataaactgt
     2521 atacacaagc accggaaaa
//
LOCUS       DBMDQJ010000357         2538 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14447_length_2538_cov_1.459992, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000357 DBMDQJ010000000
VERSION     DBMDQJ010000357.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14447_length_2538_cov_1.459992"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="ACZO9R_07770"
     CDS             <1..1378
                     /locus_tag="ACZO9R_07770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR0912997.1"
                     /translation="NVQYGCLILLVTAIGYGGGLLLAQNKVRQGKRRLAVLTGSVLLC
                     VGILFFFKYSAFAVQNINELLRAVGSVRALRVPDLILPVGISFYTFQTIGYLIDVWKG
                     KTEAEKSFLRFALFVSFFPQLVAGPIERSSSLLAQLREPAEFDYDRVKDGLLLMLWGY
                     FEKMWIADRAAILVDQVYQDYRNYSSGMLVFATVLFAVQIYCDFGGYSHIAIGAAQVL
                     GIRLMDNFRQPYFAVNCREFWRRWHISLSSWFRDYLYFPLGGSRGSSGRTAINLMITF
                     LVSGLWHGAGWHYVVWGAIHGSYQVAGRGIRYVSETAQKRLPEKKIRFFSEKTQGLTR
                     AARICVTFILTDFAWLFFRADHLGRAVAMLRRIVQNGNWLGQFTDGTLFGMGLRRLEV
                     YQLAFAIGVLLVVDILHESGISIRAWLAKQVLVIRWSIYMLVLLGFVVLLLQNYGRAA
                     AEFLYFQF"
     gene            1388..2347
                     /locus_tag="ACZO9R_07775"
     CDS             1388..2347
                     /locus_tag="ACZO9R_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912998.1"
                     /translation="MKRFTKKLIAFLGIFAVLLLTFDLLSATERFRGVFAALTDSSDY
                     EEGAEREVAAYLAKSRTPGSYTKLLVGDSVCAQMTEAFYDCNQQYCLVGNNRALTMAG
                     EYLLVKEFLETHENVSEVWLMAGPDLLQTSIDATYSYSYVVLPFLQADLLGELDEETA
                     EEMEETFGSFFLKKPVAELIAGSAVNRKLYLNYVKEREEAAKKGKSGDDRTDGMSDLA
                     ERYLRKIYELCDTQGVACYLIPDPLADTPARRKQVEQIRQDFETRGLDRLFPDYFSEI
                     TYYPADQFSDGIHFGRPYNTKEVYCEKLRELYLDRGYLDGFQI"
     gene            2331..>2538
                     /locus_tag="ACZO9R_07780"
     CDS             2331..>2538
                     /locus_tag="ACZO9R_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0912999.1"
                     /translation="MDFKYEPGRILLPVWCRMQHKEMDGIIAGQQCGTKCGTRKKYDK
                     KLDKTRYQCIIQNANELQMHVNCKN"
BASE COUNT          600 a          508 c          771 g          659 t
ORIGIN      
        1 gaatgtgcag tatggctgtc tgattctgct tgtgaccgca atcggttatg ggggcggact
       61 tttgctggca caaaataaag tacgacaagg gaagagacgt ctggcggtgc tgaccggcag
      121 tgttcttctg tgtgtcggaa tcctcttttt tttcaaatat tctgcttttg cagtgcagaa
      181 tatcaacgaa ttgctccgtg cggtgggaag cgtgcgagcg ttaagggtgc cggatctcat
      241 tcttccggtc ggaatatctt tttacacgtt ccagaccatc gggtacctga tcgatgtctg
      301 gaaagggaag acagaagcgg aaaaaagttt cctgcgtttt gcgctgtttg tgtcattttt
      361 cccgcagctt gttgcggggc cgatcgagcg gagcagcagt ctgcttgcac agctgcggga
      421 gccagctgag ttcgactatg acagggtaaa ggacggactg cttctgatgc tgtgggggta
      481 ttttgaaaaa atgtggattg cggaccgtgc tgcgattctg gtcgatcagg tctatcagga
      541 ttaccggaat tattcctccg gcatgcttgt gtttgccacg gtgctgtttg cggtacagat
      601 ctactgtgat ttcggcggtt acagccatat tgccatcggg gcggcgcagg tgcttggcat
      661 ccgcctgatg gataatttcc gacagcccta ttttgcagtg aattgccggg aattctggag
      721 acgatggcat atcagtctct cgagctggtt ccgcgactat ctgtatttcc cgctgggagg
      781 aagccgcggc agcagtggga gaaccgctat aaacctgatg ataacatttc ttgtcagcgg
      841 gctctggcat ggcgcaggct ggcactatgt ggtgtgggga gcaatccatg gaagctatca
      901 ggtggcgggc agaggcatca gatatgtaag tgagaccgcg caaaagaggc tgccggagaa
      961 aaagatacgt tttttttcgg aaaaaacgca gggactgacg cgcgctgccc ggatttgtgt
     1021 gacatttatc ctcacggatt ttgcgtggct ttttttcagg gcagatcatc tgggacgagc
     1081 tgtggcgatg cttcggcgga ttgtgcaaaa cggtaactgg ctgggacagt ttaccgatgg
     1141 cacattattt ggcatggggc ttcgcagact ggaggtgtat cagctggcat ttgccatcgg
     1201 agttctgctt gtagtggata tcctgcatga atccgggatt tccatacgtg cctggcttgc
     1261 aaagcaggtg cttgtgattc gctggagtat ttacatgctg gttcttctgg gatttgtcgt
     1321 gctgttgctg cagaattacg gaagagcggc ggcggagttt ctttattttc aattttagga
     1381 ggaaggcatg aagcgtttta caaaaaagtt gatcgccttt ttaggaatat ttgcggtact
     1441 gcttctgacc tttgatctgt tgtcggcgac ggagagattt cgcggtgtgt ttgccgcgtt
     1501 gacggacagc agtgattacg aggagggagc tgagcgggaa gtggctgcgt atcttgcaaa
     1561 gagcagaacg ccgggcagtt acacgaagct gttggtcggt gacagtgtct gcgcacagat
     1621 gacggaagcg ttttatgatt gtaatcaaca atactgtctg gtcggaaata atcgcgcgct
     1681 gaccatggcg ggggaatatc ttctagtaaa agaatttctg gagacgcatg agaatgtctc
     1741 ggaagtctgg ctgatggcgg gaccggatct gcttcagacg tcgatcgatg ccacatacag
     1801 ctattcttat gtggttcttc cgtttttgca ggcggatctg ctgggcgaac ttgacgagga
     1861 gaccgcagag gaaatggagg agacgttcgg aagctttttc ctgaaaaagc cggtggcaga
     1921 actgatcgcc ggttcggcgg taaaccgcaa gctgtatttg aattatgtga aggagcggga
     1981 ggaagcggcg aagaagggga aaagcggaga tgaccggaca gacggcatgt ctgatctggc
     2041 agagcgctat ctgcggaaaa tttacgaatt gtgcgacacg cagggggtgg catgctatct
     2101 gatccccgat ccgctggcag atacaccggc gcgccgcaag caggtggagc agatccggca
     2161 ggattttgaa acacggggat tggatcggct tttcccggat tatttttctg aaattaccta
     2221 ttatccggcg gaccagtttt cggatggaat tcattttggc agaccgtaca atacaaagga
     2281 agtgtactgc gaaaaattaa gagaactgta tctggacaga gggtatctgg atggatttca
     2341 aatatgagcc tggacggatt ctgcttcctg tttggtgtag aatgcagcac aaagaaatgg
     2401 atggaattat agccgggcag cagtgtggca ccaaatgtgg cactcgaaag aaatacgata
     2461 aaaagcttga caaaacacga tatcaatgta taatacaaaa tgcaaatgag ttgcaaatgc
     2521 atgtaaattg caaaaacg
//
LOCUS       DBMDQJ010000358         2530 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14510_length_2530_cov_1.560725, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000358 DBMDQJ010000000
VERSION     DBMDQJ010000358.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14510_length_2530_cov_1.560725"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="ACZO9R_07785"
     CDS             <1..267
                     /locus_tag="ACZO9R_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR0913000.1"
                     /translation="IHTIKPLDEELVVKTAKKTGRVFTVEEHSIIGGLGAAVAETLGE
                     KCPTKITRIGVNDVFGESGPAKELLHKYGLDAEGIANRILREMK"
     gene            297..992
                     /locus_tag="ACZO9R_07790"
     CDS             297..992
                     /locus_tag="ACZO9R_07790"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HIR0913001.1"
                     /translation="MLEELKKQVYEANMELPRRGLITYTWGNVSGIDRESGYFVIKPS
                     GVDYDALSPDDMVVMDLDGNKIEGRYKPSSDTATHIELYKKYEEIGGIVHTHSPEAVA
                     WAQAGRDIPLYGTTHADYFFGPIPCARNLTPEEIDEAYEKNTGLVIIETFETRGIKPM
                     YTPAVLCANHGPFTWGKDAAEAVHNAVVLEEVAKMARNTELINPKVKPAPDCIKEKHF
                     YRKHGANAYYGQN"
     gene            1052..2098
                     /locus_tag="ACZO9R_07795"
     CDS             1052..2098
                     /locus_tag="ACZO9R_07795"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR0913002.1"
                     /translation="MQKRSFGRTKEGREVTEYTLVNAQGMTLSVIDLGATIVSITMKG
                     SDGNVYDVVLGYDTPVDYQNNTCYFGAVIGRNANRIDHAHIHLDGKDYSLEANDNENN
                     LHSGSHGFHAVIWDVVQESAQSITFSYVSRDGEQDFPGNMTAKVTYTLDDNGEVAIAY
                     EAVCDQTTVANFTNHAYFNLDGHDSGVMEGQKLELHASYYTPVIDSKAIPTGELAKVA
                     GTPFDFREMKEIGRDIEADDTQLRYAGGYDHNYALDKADGTMQLAARARGAKSGICMD
                     VYTDCVGIQLYTGNFITPQTGKGGVQYGKRHAFCLETQYFPNAVNEKNFKSPVLKAGD
                     TYCSKTKYCFGLEK"
     gene            2283..>2530
                     /locus_tag="ACZO9R_07800"
     CDS             2283..>2530
                     /locus_tag="ACZO9R_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019162643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR0913003.1"
                     /translation="MTNEKIKRMFDACYQAKRIREMLPPLPPGVMPSYIQYLDVIRKL
                     EGQGTGVKVSDISDALNLPRPGVTRTVKEMEKKGYICK"
BASE COUNT          721 a          562 c          730 g          517 t
ORIGIN      
        1 attcacacta tcaagccgct tgacgaggaa cttgtggtaa agacggcaaa aaagacgggg
       61 cgtgtattca cggtggaaga gcattccatc atcggaggac tcggcgctgc agtggcggag
      121 acgctcggtg aaaaatgccc gacaaagatt accagaatcg gtgtcaacga tgtctttggc
      181 gaatccggtc cggcaaaaga acttcttcac aaatacggac tggatgcaga gggaatcgca
      241 aaccgcatcc tgcgtgagat gaagtagaac ggcgcagcac aggaggaaac taagagatgt
      301 tagaagaatt aaaaaaacag gtctatgaag ccaacatgga acttccgcgc agagggctca
      361 tcacctacac ctggggcaat gtcagcggaa tcgaccgcga gagcggctat ttcgtcatca
      421 agccgagcgg tgtggattat gatgccttaa gcccggacga tatggtagtc atggatcttg
      481 acggaaataa gatcgagggc agatataagc cgtcctccga cacggcgacc catatcgaac
      541 tctacaaaaa gtacgaggag atcggtggta ttgtacatac ccattccccg gaggctgtcg
      601 cgtgggcgca ggcgggcaga gacattccgc tttacggcac aacgcatgca gactatttct
      661 tcggaccgat tccgtgcgcc agaaatctga cgccggagga gatcgacgag gcatatgaga
      721 agaacaccgg acttgtgatt attgagacct ttgagacaag aggcatcaag ccgatgtaca
      781 caccggcggt tctctgtgcg aatcacggac cgtttacctg gggcaaggat gccgcagagg
      841 cagtccacaa tgcggtcgta ctggaggaag tggcgaagat ggcacgcaac actgagctta
      901 tcaatccgaa ggtgaagccg gcgccggatt gtatcaagga aaagcatttc taccgcaagc
      961 atggggcaaa tgcctactat ggacagaatt agtacgtcgg aagaaacggc ctgccgtttc
     1021 gcaataaata tgcttagaaa tgaggatcag tatgcaaaaa cggagttttg gcagaacaaa
     1081 ggaaggcagg gaggtcacgg agtacacgct tgtcaatgcg cagggaatga cgctctctgt
     1141 cattgatctc ggggcaacga tcgtatcgat cacaatgaag ggctcagacg gaaacgtata
     1201 tgacgttgtg ctgggatacg atacacccgt tgattatcag aacaatacct gctattttgg
     1261 agcggtgatc gggcgcaatg cgaaccggat cgatcatgca cacatccatc tggacggaaa
     1321 agactattcg ctggaagcga atgacaatga gaacaatctg cacagcggat cgcacggttt
     1381 tcacgcagtg atctgggatg tggtgcagga gagcgcacag agtattacgt tttcctatgt
     1441 aagccgcgac ggggaacagg atttcccggg caatatgacg gcgaaggtta cgtacacgct
     1501 ggatgataac ggggaggttg cgatcgccta tgaggcagtc tgcgaccaga cgacggtggc
     1561 gaactttacg aaccatgcct actttaatct ggacggacat gattccggcg tgatggaagg
     1621 gcagaagctc gaacttcacg catcctacta tacgccggtc attgattcca aggcgattcc
     1681 aaccggagaa ctcgcaaaag tagccgggac gccgtttgat ttccgggaga tgaaggagat
     1741 cggacgggac atcgaagcgg acgatacgca gcttcgttat gcgggtggtt atgaccacaa
     1801 ttatgcgctg gacaaggcgg acggtaccat gcagcttgct gcgcgtgcca gaggtgcaaa
     1861 gtccggcatc tgcatggatg tgtatacgga ctgtgtgggg atccagctct ataccggcaa
     1921 ctttatcacg ccgcagaccg gcaagggcgg cgtgcagtac ggcaagcgcc atgcattctg
     1981 tctggagacg cagtatttcc cgaatgcggt gaatgagaag aacttcaaat caccggtatt
     2041 aaaagcgggc gatacgtact gctcaaagac aaagtattgt ttcgggctgg aaaaataaga
     2101 acaacgaatc aggaatgaga gaaagtggat tttatcacag aaaacggtgg taagatccac
     2161 tttttttgtt ttgcagggaa aacagcgtcc atacttgaca aaaaggaaat cctgtctata
     2221 ataattactg ttggtaatta cctttaataa ctaaatggag cgacgagctg ggaggataaa
     2281 tcatgacgaa cgaaaaaatc aagcggatgt ttgatgcctg ctatcaggcg aagcggatca
     2341 gagaaatgct tccgcctctg ccgccgggcg tgatgccgtc ttatattcaa tatctggatg
     2401 tgattcggaa gctggaaggg cagggcaccg gggtaaaggt atcggacatc agcgatgcgc
     2461 tcaatcttcc gcgccccggt gtgacccgaa ccgtaaaaga gatggaaaaa aagggatata
     2521 tctgtaagat
//
LOCUS       DBMDQJ010000359         2529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14524_length_2529_cov_1.480643, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000359 DBMDQJ010000000
VERSION     DBMDQJ010000359.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14524_length_2529_cov_1.480643"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            198..1292
                     /locus_tag="ACZO9R_07805"
     CDS             198..1292
                     /locus_tag="ACZO9R_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0913004.1"
                     /translation="MSEKTKDVLLSIFASLLCIVIGLLVGLIILYCINAENALDGFSR
                     IIKGGFYLKPKGIGSEIAQATPLIMTGLSVAFAFKTGLFNIGAAGQYTLGVFGALYFA
                     LILQMPWYVCLLAAMVCGAIWGAVPGIFKAYFNVNEVITSIMFNWIGLYLVNELMYST
                     VSGMYDQKTTRTYKLSSAAPQSLIPDCGMSAVFKTSSTTIAIFIAMAIAIIMYVVLNK
                     TTFGYELKACGFNKDAAKYAGINEKRNIVLSMTISGALAGIGAGLYYLSGASEWNPQV
                     STALPAIGFNGIPVALLALSNPIGVVFAAIFVAHISVGGAYLPTKYYQPEIADLIVAV
                     IIYLCAFVMLFKSVVVSKLSRKKAKKEDVK"
     gene            1292..2230
                     /locus_tag="ACZO9R_07810"
     CDS             1292..2230
                     /locus_tag="ACZO9R_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0913005.1"
                     /translation="MFLLIKFTLLYAVVLMLVALGGMFSERSGVINIALEGIMAIGGL
                     AGVFVLALCGDSLPSGVLVAVSILASAVAGILYSLLLGFSAITLKADQTIGGTALNML
                     ATAVAIVAVKAFNNSSAAGNGAASAKLSYSNKAFIYKIGPLTINIFLIIGIIILIVSY
                     VFLFKTKWGLRLRACGEHPQAADSVGINVYQWRYTGVMISGALGGIGGFAYIVPSVTT
                     WNFEVGVAGAGFLALAVMIFGQWKPFNIAGAAVFFAVFRSLANIADSTVLANLGLSKN
                     IYSMMPFIASMIILAFTSKNSQAPKAEGIPYDKGAR"
     gene            2277..>2529
                     /locus_tag="ACZO9R_07815"
     CDS             2277..>2529
                     /locus_tag="ACZO9R_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009218963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913006.1"
                     /translation="MITNRKDSKIMISSKYFDHTILKAEATETQVAKICDEALANDFA
                     SVCVNQYYTRFVAEKLKGSDVKVCTVVGFPLGMSDTGVKA"
BASE COUNT          606 a          552 c          696 g          675 t
ORIGIN      
        1 cgttggagct ggatgaagtt atggacgtgt ccgaccgtat cctggtgatg tacgagggtg
       61 agatcgtagg agaactggat ccgaagaaag tgactgtgga tgaacttgga ctttacatgg
      121 caggtgcaaa gcgggatgag gttgcaaaga aagcataaag gcgaggcctt acagattaac
      181 agaaaggaaa tatgacaatg agtgaaaaga caaaggatgt cctattgtcc atctttgcat
      241 cgcttctttg cattgtgatc ggacttctcg tgggacttat catactttat tgtatcaatg
      301 cagagaatgc attggatggt ttttcaagaa ttatcaaggg tggcttttac ctcaaaccaa
      361 agggaatcgg aagtgagatt gcgcaggcga caccgctgat catgacgggt ctttccgttg
      421 cgtttgcttt taagacggga ctgttcaaca tcggtgcggc aggccagtat accttgggcg
      481 tgttcggtgc attgtatttt gcactgattc tgcagatgcc gtggtatgta tgtcttctgg
      541 cagcaatggt ctgcggagcc atctggggtg ctgttccggg aattttcaag gcgtacttta
      601 atgtcaatga ggttatcacc tccatcatgt ttaactggat cggtttatat ctggtgaacg
      661 agctgatgta cagcacggta tccggaatgt acgaccagaa gacgacgcgt acttacaagc
      721 tgagttccgc agcaccgcag tcattgattc cagattgcgg catgagcgct gtttttaaga
      781 caagctcgac cacgattgcc atttttatcg cgatggcgat cgcaattatc atgtatgtgg
      841 tactcaataa gaccacattc ggttatgagt taaaagcctg tggctttaac aaagacgccg
      901 caaaatatgc gggcatcaat gaaaaacgca acattgtgct ttccatgaca atctccggcg
      961 cacttgcagg tatcggtgcg ggactttact atctgtccgg tgcatccgag tggaatccgc
     1021 aggtgtccac agcgcttccg gcgatcggtt tcaacggtat cccggtagca cttttggcgc
     1081 tgagcaatcc gatcggcgtg gtatttgcag caatctttgt cgcacatatc tccgtgggag
     1141 gcgcgtatct accaaccaag tattatcagc cggagattgc ggatctgatc gtggcagtca
     1201 tcatttacct gtgtgcgttt gttatgctct ttaagagcgt ggtcgtatcg aagctcagca
     1261 ggaaaaaagc gaagaaggag gatgtgaaat aatgttctta ctgattaagt ttacattact
     1321 ctatgcggta gtattgatgc tggtggcgct tggcggtatg ttctcggagc gaagcggtgt
     1381 aatcaacatt gcacttgaag gtatcatggc aatcggcggt ctggcgggcg ttttcgtcct
     1441 ggcactctgc ggggattccc ttccgtccgg cgtgctggtg gcggtgtcca tcctggcaag
     1501 tgcggtagcg ggaattctct attcccttct gctcggattt tccgccatca cgttaaaggc
     1561 ggatcagaca atcggcggaa cggctttaaa tatgcttgcc acagcggtgg cgatcgtagc
     1621 ggtaaaagcg ttcaacaact cctcggcagc gggaaacggt gcggcttccg caaagctttc
     1681 ctacagcaac aaggcattta tctataaaat cggaccgctc acgatcaaca ttttcctgat
     1741 tatcggaatt atcattctga ttgtaagcta tgtatttttg ttcaagacca aatggggctt
     1801 acgtctgcgc gcgtgcggtg aacatccgca ggcagccgat tcggtcggta tcaatgtgta
     1861 tcagtggaga tataccggtg tcatgatctc gggtgcactc ggcggaatcg gaggcttcgc
     1921 ctacatcgtt ccatccgtta ccacctggaa ctttgaggtc ggcgtagcgg gagccggttt
     1981 cctcgccttg gcagttatga ttttcggaca gtggaagccg ttcaacattg cgggcgcagc
     2041 cgtgttcttt gcggtattcc gttccctggc gaacatcgcg gattccaccg tgcttgcaaa
     2101 cctcggattg tccaagaata tctacagcat gatgccgttt atcgcatcca tgattattct
     2161 tgcgtttaca tccaagaatt cccaggcacc gaaagcggag ggtattccgt atgataaggg
     2221 tgccagatag gaagacggcg taagagaccg gaatttccgg aagcgtccgg gagcatatga
     2281 taacaaacag aaaggattct aagatcatga tttcaagcaa atattttgac catactattt
     2341 taaaggcaga ggcgacggag acacaggtgg caaagatctg tgatgaggcg cttgccaatg
     2401 atttcgcatc cgtgtgcgtg aaccagtatt acacccgttt tgtggcagag aagttaaaag
     2461 gttctgacgt gaaggtgtgc acagtggtag gtttccctct gggaatgtcc gacaccggtg
     2521 tgaaggcat
//
LOCUS       DBMDQJ010000360         2529 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14526_length_2529_cov_0.932867, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000360 DBMDQJ010000000
VERSION     DBMDQJ010000360.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14526_length_2529_cov_0.932867"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="ACZO9R_07820"
     CDS             <1..425
                     /locus_tag="ACZO9R_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4867 family protein"
                     /protein_id="HIR0913007.1"
                     /translation="LPIQIGYCNGSNHRLNALEYHRNSEINIAVTNMVLLLGWLPDVT
                     DAFTYDTSKVEAFFVPAGTVVEMYGTTLHYAPCNDGEEGFKCIVILPKGTNTEIDFAL
                     PKSGEDALMTAKNKWLIAHEEAGIRGAFNGLMGVNIEV"
     gene            487..1977
                     /locus_tag="ACZO9R_07825"
     CDS             487..1977
                     /locus_tag="ACZO9R_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
                     /protein_id="HIR0913008.1"
                     /translation="MNKENMPEIKIGVVAVSRDCFPESLSVNRRKALMDAYTKKYGKD
                     HIYECPICIVESEIHMVQALEDVKAAGCDALVVYLGNFGPEIAETLLAKHFDGPKMFI
                     AAAEESGDSLLDGRGDAYCGMLNASYNLKLRNIRAYIPEYPVGTAEECADMIHEFEPI
                     ARAVVALSNLKIISFGPRPLNFLACNAPIKQLYNLGVEIEENSELDLFEAFHKHDGDE
                     RIPAVVAEMEAELGAGNKKPEVLAKLAQYELTLKDWVRDHKGYRKYVAIAGKCWPAFQ
                     TQFGFVPCYVNSRLTGQGIPVSCEVDIYGCLSEFIGTVVSEDAVTLLDINNSVPADMY
                     EEEIEGKYPYTLKDTFMGFHCGNTTSGKLAFCEMRNQKIMARSLPVEVTNGTLEGDIM
                     PGDITFFRLQSTADCELRAYIAHGEVLPVATRSFGSIGVFAIPEMGRFYRHVLIEKNF
                     PHHGAVAFGHYGKALYEVFKYIGVPVEEIGYNQPAGVRYPTENPFA"
     gene            2051..>2529
                     /locus_tag="ACZO9R_07830"
     CDS             2051..>2529
                     /locus_tag="ACZO9R_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR0913009.1"
                     /translation="MLYIGIDLGTSAVKLLLMDADGKIKKIASRTYDLSFPHPGWSEQ
                     NPADWYEQSIDGLKELLAECDKSRVAGISFGGQMHGLVTLDERDEVIRPAILWNDGRT
                     AEETDYLNQVIGKETLSGYTANIAVAGFTAPKILWMQKHEPDLWKRVKKIMLPKDYLA
                     "
BASE COUNT          686 a          503 c          754 g          586 t
ORIGIN      
        1 gacttccgat tcagatcggt tattgtaacg gcagcaatca cagattaaat gcgttggagt
       61 atcaccgcaa ttcggagatc aatattgcgg tgaccaatat ggtgctgctg ttaggatggc
      121 tgccggatgt gaccgatgcg tttacttatg acacatccaa ggtggaagca ttttttgtcc
      181 cggcgggaac ggttgttgag atgtatggaa cgacacttca ctatgcgccg tgcaatgacg
      241 gggaggaagg atttaaatgt attgtgattc ttccgaaagg aacgaataca gagattgatt
      301 ttgccttgcc caaaagcgga gaggatgcgc ttatgactgc gaagaacaag tggctgatcg
      361 cacatgagga agcaggaatc cgtggtgctt ttaacggact aatgggggtc aatattgagg
      421 tgtaagaaag atacaggaaa aacggaacag gaaggtttct atttttgaaa cggaaaggag
      481 atttttatga acaaagaaaa tatgccggag attaagatcg gagttgtggc agtcagcaga
      541 gactgcttcc cggagagcct gtcggtgaac cggagaaagg cgttaatgga cgcttacacg
      601 aaaaagtacg gaaaggatca catctatgaa tgtccgatct gtatcgtgga gagtgaaata
      661 catatggtgc aggcgctgga ggatgtgaag gcggccgggt gtgatgcact tgtcgtatac
      721 ctcggcaatt ttggaccgga gatcgccgag acactgctgg caaagcattt tgacggaccg
      781 aaaatgttta ttgctgcggc ggaagaatcc ggggacagtc tgctggatgg cagaggggat
      841 gcttactgtg gaatgttaaa tgccagctat aacttaaagc ttcgcaatat cagggcatac
      901 attccggagt atccggttgg aacggcagag gaatgtgcgg acatgattca tgaatttgag
      961 ccgatcgccc gcgcggtagt tgcgctttcc aatttaaaga tcataagctt cggaccaaga
     1021 ccgttaaatt tccttgcctg caacgcaccg ataaagcagc tttacaatct gggtgtggag
     1081 attgaggaaa attcagagct tgatttattc gaggcgttcc ataaacatga cggggatgag
     1141 agaatcccgg cggttgtggc agagatggag gcggagcttg gagccgggaa taaaaagccg
     1201 gaagtgcttg caaagctggc gcagtatgaa ctgaccttaa aagactgggt gcgcgatcac
     1261 aagggctacc gcaagtatgt ggcgattgcg ggcaagtgct ggccggcatt ccagacacag
     1321 ttcggttttg tcccgtgtta cgtcaacagc cgtctgaccg gacagggaat tccggtgtcc
     1381 tgcgaggtgg atatttacgg ctgtttaagc gagttcatcg gcaccgtggt gagcgaagat
     1441 gccgtgacac tgcttgatat caataactcg gtgccggcag atatgtatga ggaagagatc
     1501 gagggaaaat atccatatac gttaaaggac acctttatgg gattccactg tggaaatacc
     1561 acttccggaa agcttgcgtt ctgtgagatg cgcaatcaga agatcatggc gagaagcctt
     1621 ccggtcgagg tgacaaacgg aacccttgag ggggacatca tgccggggga cattacgttt
     1681 ttccgtctgc agagcaccgc agactgcgag ctccgcgctt atatcgcaca tggggaggta
     1741 cttccggtgg cgacaagatc ttttggatcg atcggtgttt tcgccatccc tgagatggga
     1801 agattttacc gccatgtgct gattgagaag aatttcccgc accacggcgc ggtggcattc
     1861 ggacattacg gcaaggcgct gtacgaagta tttaagtata tcggtgtgcc ggtggaggag
     1921 atcggttata accagccggc aggcgtgcgc tatccgacgg agaatccgtt tgcatagaaa
     1981 agtcttgcaa tgcatagcga agtcttgcag tgcatagcga agtcttgcaa tgataagaaa
     2041 aggggagagt atgttatata taggaattga tcttggcaca tcagcagtaa aattgctgct
     2101 gatggatgcc gatggtaaaa taaaaaaaat tgcatcgcgc acctacgatc tttcatttcc
     2161 acatccgggc tggtcggaac agaatccggc agactggtat gaacagtcga tcgacggctt
     2221 aaaagaactt ctggcggaat gtgataaaag ccgggtggcg gggatcagct tcggggggca
     2281 gatgcatggt ctggtgacat tggatgagcg ggatgaagtg attcgtccgg cgatcctctg
     2341 gaacgacggg cgcacggcag aagagaccga ttacttaaat caggtgatcg gaaaggaaac
     2401 actgtcggga tataccgcga atattgccgt cgccggattt acggcgccaa agattctctg
     2461 gatgcaaaag catgagccag atctgtggaa acgggtgaaa aagatcatgc taccgaaaga
     2521 ttatctcgc
//
LOCUS       DBMDQJ010000361         2519 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14611_length_2519_cov_1.437965, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000361 DBMDQJ010000000
VERSION     DBMDQJ010000361.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14611_length_2519_cov_1.437965"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="ACZO9R_07835"
     CDS             complement(<1..978)
                     /locus_tag="ACZO9R_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR0913010.1"
                     /translation="MGKIFKNMIPYWKPLILVLLLLFVQAWCDLSLPAYTSDIIDVGI
                     QNQGIKHILPEALTPEEYEKAELLMTDEETASWEESYALNGSVYERNVTGEERLEELD
                     DTFLVPLLMNYQMSAMEESTFKKLMAEQMHTDESALDGMTIEQIGASMGVELKTFEKK
                     TQNEDGNTTTVTCVDMRDLLQAMVSGGMLEKDQLLSMRGELNSMLDAMGSSLVKSMGV
                     AYAAGCDAAAGVDVEAIQKSYLWKAGLKMVGMALIMGIATVLVGFIGARIGAGIGRDL
                     RGKIFKRVVSFSNAEMDRFSTASLITRSTNDIQQIQMTCAILIRMVAYAP"
     gene            complement(962..1447)
                     /locus_tag="ACZO9R_07840"
     CDS             complement(962..1447)
                     /locus_tag="ACZO9R_07840"
                     /inference="COORDINATES: protein motif:HMM:NF047172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR0913011.1"
                     /translation="MEELYAGLFRAMNRFGKLRIGELFSDMTKSDGMMLWAISQLNQE
                     KNNGRATVSELAEKLHTQNSAVSRTLKNLEDHGWICRMTDPKDRRNTCVALTESGEDM
                     QQNMTCTLQEFGRAVISQMDEQDLKKLIVCLDELYDIAAEEIEKRSMRNRKEGQHGKD
                     F"
     gene            complement(1560..2327)
                     /gene="aroD"
                     /locus_tag="ACZO9R_07845"
     CDS             complement(1560..2327)
                     /gene="aroD"
                     /locus_tag="ACZO9R_07845"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HIR0913012.1"
                     /translation="MKPLEIKHRVIGGGEPLICVPVVEQDAQSVIREITYLSQSTADM
                     IEWRVDAFRDFTDYNAIRSVFEAVAPLLGEKLFLYTFRTAHQGGMVQVKAEQLDDLHD
                     LAAESGCVDLVDLEFFEEDRPLHKIKRLREMGVSVIASHHDFEQTPSPEVMKMLLEKM
                     CAGGADIVKLAVMPQNYKDVLNLLDVTAQFREENPDTPVITMSMGSLGGISRISGETF
                     GSCVTFGAHEKPSAPGQFAMKELRQMLDTIHASSRIQ"
BASE COUNT          576 a          742 c          485 g          716 t
ORIGIN      
        1 cggtgcataa gctaccatac ggatcagaat cgcgcatgtc atctgaatct gctggatatc
       61 gttcgtactc cgggtaatta acgaagctgt tgaaaaacga tccatctcgg cattggaaaa
      121 gcttaccacc cttttaaata tttttccgcg gagatcacgt ccgattcctg caccgatgcg
      181 ggcgccgata aagccgacta agaccgtggc aatccccata atcagagcca ttcccaccat
      241 tttcaaaccg gctttccaca gataagactt ctgaattgct tccacatcca cgcctgccgc
      301 cgcatcgcag cctgccgcgt acgccacgcc catcgatttg acaagcgaac tgcccatggc
      361 atcgagcatc gagttgagtt ccccgcgcat tgataaaagc tggtcttttt ccagcatgcc
      421 gccggatacc atcgcctgca gcagatctct catatccaca caggtgactg tcgtcgtatt
      481 tccgtcctcg ttctgcgtct ttttttcaaa tgtttttaat tccacgccca tggacgcgcc
      541 gatctgctcg atcgtcattc cgtccagcgc agactcatct gtatgcatct gctctgccat
      601 cagtttctta aatgtacttt cctccatagc agacatctga taattcataa gaagcggtac
      661 gagaaacgta tcatccagtt cctccagccg ttcttccccg gtcacattcc gctcgtacac
      721 actgccattt aacgcatagc tttcctccca ggatgccgtc tcttcatccg tcatcaaaag
      781 ttctgccttc tcatactcct ccggtgtcaa tgcctccggc aggatgtgtt ttattccctg
      841 attctgaatt ccgacgtcaa taatgtccga tgtgtatgcc ggcagtgaca gatcgcacca
      901 cgcctgcaca aacaacagca ataacaccag gatcagcggt ttccagtatg gaatcatatt
      961 tttaaaaatc tttcccatgt tgtccttcct ttctgtttcg catacttctc ttctcgattt
     1021 cttctgctgc aatgtcatat aattcatcca ggcatactat gagctttttg agatcctgtt
     1081 catccatctg cgaaatgact gccctgccaa attcctgcaa ggtgcatgtc atattttgct
     1141 gcatgtcctc cccactctcc gtcagagcga cacaggtatt gcgccggtct ttcggatctg
     1201 tcattctgca gatccagccg tgatcctcca gattcttcaa agtccttgac acagcagaat
     1261 tctgggtatg aagcttctct gccagttcag acactgtagc tctcccatta ttcttttcct
     1321 ggttcaactg tgaaattgcc cagagcatca tgccgtcgct ttttgtcata tcggaaaaca
     1381 gttctccgat ccgcagtttt ccgaaacggt tcatcgcccg aaataaaccc gcatacaatt
     1441 cttccatatt tcaccacctt gttaattatt aacactgtta cataatttgg gacattataa
     1501 tagcacaggg agattgctgc gtcaagcaat ctccctgtgt ttatcttttt ttaataattc
     1561 tattgtatcc ggctgctggc atgaatcgta tccagcatct gtctgagttc tttcatggcg
     1621 aactgtcccg gcgcactcgg cttttcgtgc gcgccgaatg tgacgcagga gccgaacgtc
     1681 tccccactga tcctgctgat gccgccgagt gatcccatgg acatcgtaat caccggcgta
     1741 tccggattct cctctctaaa ctgtgccgtc acatccagca gattgagcac gtctttataa
     1801 ttctgcggca tcaccgcaag ctttacaata tccgctccgc cggcgcacat tttctccagc
     1861 agcatcttca tgacttccgg agacggcgtc tgctcaaaat cgtgatgtga ggcaatgacc
     1921 gacactccca tctcccgcag cctttttatc ttatggagcg gtcggtcttc ctcaaagaat
     1981 tccagatcca ccagatcgac acagccggat tccgccgcaa gatcgtgcag atcgtcgagc
     2041 tgttccgctt tcacctgcac catgcctccc tgatgcgcgg tacggaatgt atacaggaaa
     2101 agcttctccc ccagcagagg cgccacagcc tcaaatacgc tccggattgc attgtaatct
     2161 gtaaaatcgc ggaatgcatc cactctccac tcgatcatat ccgcggtgct ctgactcaga
     2221 tacgtgatct cccggatcac cgactgtgca tcctgctcta ccacgggaac gcagatcaac
     2281 ggttcccctc ccccaatcac acggtgtttt atctccaatg gcttcatgtt cctgctccct
     2341 ttccatacct gcattttttc atccggacca cttccatgca cggctttttt tacagtttac
     2401 tccattctga ataaatatgc aaatatttga catctattgt aatatcagct aagataatta
     2461 cgattcaaac agaaaggagt gtgcggtgtc tttttcctta tccacacaat gtcatatga
//
LOCUS       DBMDQJ010000362         2519 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14612_length_2519_cov_1.373863, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000362 DBMDQJ010000000
VERSION     DBMDQJ010000362.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2519)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14612_length_2519_cov_1.373863"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="ACZO9R_07850"
     CDS             complement(<1..896)
                     /locus_tag="ACZO9R_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR0913013.1"
                     /translation="MESMDDYKEELEASFRRIREGDILTGTVIDVNEEGVTLDLKYYA
                     PGFISAEEMSSDPDFSLAEAVHVGDTIEATVIRVDDGAGNILLSRREANEEQAWEKLK
                     EMMEEGTTVTVRIKESVKAGVVAYLEGIRAFIPASQITSAYVEDTESWVGKEIPVRVI
                     TVDEEREKLVLSGKAVALEAEAEERNHKISMIVPGTVLEGTVESLMPYGAFIDLGNGL
                     SGLVHISQICERRIGKPSEVLKVGEKVKAKVLNTNDNKISLSMKALAEEMVDTEGVED
                     LEAYTDHTSTGTSLGSLLAGLK"
     gene            complement(902..>2519)
                     /locus_tag="ACZO9R_07855"
     CDS             complement(902..>2519)
                     /locus_tag="ACZO9R_07855"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0913014.1"
                     /translation="AGQLQGPKDFKVIFGLPVSKSDTISLGDPYEGMTNYWVQVVLVY
                     RASGGSILFLVSLAAVAGWMFLMTSRRIWNEDKPMQRPGKWYLMEVAVIAVCCMACME
                     ENFYDIIRSYSYYSEGVDVLLGNNAWRAILQLGQMMVPLLFLYGIWYAALRVLRPVFF
                     LGVREYIRQYSLIYQIFPWLKKLWNTFRYEVAHVDFSEKSTKTIWKIVLINFAVLSLC
                     SMMWFFGIGALIVYSIFVFCFIKKYYDRIGRDYQALLRSVSRMADGDLDTEITEDLGI
                     YEPMKVELSRVRDGFKKAVDEEVKSQRMKTDLITNVSHDLKTPLTAITTYVELLKKED
                     ITEEERRSYIETLDKKSQRLKILIEDLFEVSKATSNNIVLHPMEVDVVNLLKQVAVEH
                     TERFAAMGLTLRWDVPEEKVIVVLDNQKTFRIFENLFVNIQKYAMPQSRVYVQVHTSG
                     EAPEEFVEITIKNMSATELNFDPEEITERFVRGDTSRGTEGSGLGLAIARSFTEAQGG
                     KLSIEVDGDLFKVVIRLKCGTLQVEKEMEV"
BASE COUNT          564 a          791 c          508 g          656 t
ORIGIN      
        1 aacttcaatc ctgccagaag cgagccaagg ctggttcctg tggatgtgtg gtctgtgtat
       61 gcctccagat cttccacacc ttcggtatct accatctcct ctgccagcgc cttcatggac
      121 aggctgatct tattgtcgtt ggtatttaat acctttgcct tgaccttctc tccgacctta
      181 agcacttcgg acggctttcc gattctgcgc tcacagatct gtgagatgtg caccagtccg
      241 ctcagtccgt tgcccagatc aataaatgcg ccgtacggca tcaggctctc gacagttccc
      301 tcaagcacgg ttcccggcac gatcatagag atcttgtgat tgcgctcctc cgcctctgcc
      361 tcgagtgcga ccgcctttcc ggaaagcacg agcttttcgc gctcctcatc caccgtgatg
      421 acccggaccg gaatctcttt tcctacccag ctctccgtat cctccacata agcggatgtg
      481 atctgggatg ccggaatgaa cgcgcggatc ccctcaagat atgccacaac gcctgccttc
      541 acgctctcct tgatgcggac ggttaccgtt gtaccctcct ccatcatctc tttgagcttc
      601 tcccatgcct gctcttcatt ggcttccctg cgggagagaa gaatgttgcc tgcgccgtcg
      661 tctacccgga tcacggtcgc ctcgatcgta tcccccacat gcacagcctc cgccagtgaa
      721 aaatccggat cgctgctcat ctcctccgca ctgataaatc ccggtgcata atatttcagg
      781 tcaagcgtaa cgccctcctc gttgacatcg atcaccgtgc cggttaaaat atctccctct
      841 ctgattctgc ggaaagaagc ttctaactcc tccttgtaat catccattga ttccatgtgt
      901 cttaaacctc catctctttt tctacctgta gtgtaccaca tttcagtctg attacaacct
      961 tgaataaatc accatccacc tcgattgaca gttttccgcc ctgcgcctcg gtaaaacttc
     1021 tggcaatcgc aagtcccaga cccgagcctt ccgtgccgcg cgaagtatcg ccgcgcacga
     1081 agcgctccgt gatctcctcc ggatcaaaat tcagttccgt cgccgacata tttttgatcg
     1141 tgatctccac gaactcctcc ggtgcctccc cggatgtgtg cacctgcacg tacacgcggc
     1201 tctgcggcat cgcatatttc tgaatattga caaacagatt ttcaaagatg cggaatgtct
     1261 tctgattgtc gagcaccacg atcacttttt cctccggcac atcccagcgc agcgtaagtc
     1321 ccatcgccgc aaagcgctcc gtatgctcca ccgcaacctg ctttaacaga ttcaccacat
     1381 cgacttccat cggatgcagc acaatattgt tgctggtcgc cttgctgacc tcaaaaagat
     1441 cctcgatcaa tatttttaac cgctggctct ttttatcgag cgtctcaatg tatgacctgc
     1501 gctcctcctc cgtgatgtcc tccttcttta acagttccac gtaggttgtg atcgccgtga
     1561 gcggtgtttt taaatcatgg gaaacgttcg tgatgagatc cgttttcatt cgctggcttt
     1621 tcacttcctc atccaccgcc ttcttaaatc cgtcacgcac cctggaaagt tccaccttca
     1681 tcggctcata tattcccaga tcctcggtga tctccgtgtc cagatctccg tccgccatcc
     1741 ggcttacact gcgcagcagc gcctgatagt ctctgccgat cctgtcataa tattttttga
     1801 taaaacagaa cacaaagatg gagtacacga tcagcgcgcc gatgccgaaa aaccacatca
     1861 tcgagcagag cgacagcacc gcaaagttga tcagcacgat tttccagatc gtctttgtgg
     1921 atttttctga aaaatccaca tgcgccacct catagcggaa cgtgttccac aattttttca
     1981 accacggaaa aatctgataa atcaggctgt actgtctgat atattccctg actcccagga
     2041 aaaataccgg acgcagtacg cgaagcgccg cgtaccagat gccatacaaa aacaggagcg
     2101 gaaccatcat ctgtccaagc tgtaagatgg ctctccaggc attgtttcca agtaaaacat
     2161 ccactccttc gctgtaatag gaatagcttc ttatgatatc gtagaagttt tcctccatac
     2221 atgccatgca gcagacggca atcaccgcca cctccatgag ataccatttc cctgggcgtt
     2281 gcattggttt gtcttcgttc cagatgcgcc tgctcgtcat aagaaacatc catcccgcca
     2341 ccgcagcaag gcttaccaga aacaaaatgc ttccgccgga cgcgcggtac accagcacca
     2401 cctgcaccca gtaatttgtc ataccctcat acggatctcc caaggaaatc gtatcgctct
     2461 ttgacaccgg aagcccgaag atcaccttaa agtccttcgg tccctgcaac tgacccgct
//
LOCUS       DBMDQJ010000363         2516 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14631_length_2516_cov_1.407453, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000363 DBMDQJ010000000
VERSION     DBMDQJ010000363.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14631_length_2516_cov_1.407453"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(35..949)
                     /gene="pfkB"
                     /locus_tag="ACZO9R_07860"
     CDS             complement(35..949)
                     /gene="pfkB"
                     /locus_tag="ACZO9R_07860"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HIR0913015.1"
                     /translation="MIYTLTFNPSLDYIVTVPKFTCGVVNRTTEEIIFPGGKGINVSM
                     VLKNLGFENTALGFYAGFTGMELKRLVADKGIHAEFIPVDKGMTRINVKLRSEQESEI
                     NGQGPAIAASDIDKLYEKLDALCDGDILVMAGSIPDVMPQTMYMDIMKHLQGRNLKIV
                     VDATRDLLVNVLPFHPFLIKPNNHELGEIFGVEICEKEDVVTYAKKLQERGARNVLIS
                     MAGDGAVLVAEDGSVYQAEAPEGKLINSVGAGDSMVAGFLAGYLEHGTYDKAFQMGVC
                     TGSASAFSEELATKAEVLDLLEKNQNLF"
     gene            complement(966..1712)
                     /locus_tag="ACZO9R_07865"
     CDS             complement(966..1712)
                     /locus_tag="ACZO9R_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HIR0913016.1"
                     /translation="MLTEERFAKILSILEHMGSVTVQQLMTELDASESTVRRDLNTLD
                     ANGRLVKVHGGAILKNTVYSTIDDEVVYRKEQNREAKDKIARYAAGLITAEDFVYIDA
                     GTTTERMIDYIANRQAVFVTNAIGHAKKLAEHGCKVYILGGEFKAVTEAIVGEEAVFT
                     LDKYNFTKGFWGANGVSLQRGFSTPELKEAMVKRKSMENCRDCYILADESKFSQISSV
                     TFAPFEKATVITTELKQEIYKDCSNIINIE"
     gene            complement(1912..>2516)
                     /locus_tag="ACZO9R_07870"
     CDS             complement(1912..>2516)
                     /locus_tag="ACZO9R_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR0913017.1"
                     /translation="FYFTRHGETEWNVKKKIQGTTDIPLDEKGIQQAKRLAETLLEKQ
                     RNGELHLDRVYTSPQLRAAETARFSAEALGIDCIRLWDLREMDLGDWEGRNWDEIRET
                     EAERHHIWDTHRRFCRTPGGECYNEVLRRTLAALEQILAREKDDVLVVTHSAVLMALR
                     CYLANRPFDVMREYRTRNTELVRVTEEEIREAIRRYGRAI"
BASE COUNT          581 a          767 c          472 g          696 t
ORIGIN      
        1 tgcgcacatt ttataattct catttgccgc aaaattaaaa caagttttgg ttcttctcca
       61 gcagatcaag tacctctgcc tttgttgcaa gctcttcaga aaaagcggat gcacttcctg
      121 tacatacccc catctgaaat gccttgtcat aggttccatg ctccaaatat cctgcaagaa
      181 agcctgccac catggaatcg ccggctccga cagaattgat cagctttccc tccggtgcct
      241 ctgcctgata caccgatccg tcctcggcaa caagtaccgc cccgtctcct gccatcgaaa
      301 taagcacgtt tctggccccc ctctcctgca gtttcttcgc gtaagtcaca acatcctctt
      361 tctcgcagat ctccacgcca aaaatctctc ccagttcgtg attgttcggt ttaattaaaa
      421 acgggtggaa cggaagtaca tttaccagaa gatctctcgt ggcatccacc acaattttta
      481 aattcctgcc ctgcagatgc ttcatgatat ccatgtacat cgtctgcggc atcacatccg
      541 gaatacttcc cgccatgaca agtatgtcgc cgtcacacag tgcatccagc ttttcatata
      601 atttatcaat atcagaagca gcgatcgccg gcccctgtcc gttaatctcg ctctcctgct
      661 ctgaccggag cttgacattg atccgcgtca ttcccttatc taccgggata aattcagcat
      721 gaattccctt atcagccacc agccgcttca gctccatgcc ggtaaatccc gcataaaagc
      781 caagtgctgt attctcaaag ccaaggttct tcaacaccat ggatacgttg attccttttc
      841 caccggggaa aataatctct tccgtggtgc ggttaaccac cccgcaggta aacttcggaa
      901 cagtcacaat gtaatctaaa gatggattaa atgttaacgt ataaatcatt acctaagatc
      961 tcctttcact cgatattgat gatattggaa caatccttgt atatttcctg ttttaactct
     1021 gtcgtaatca cagtcgcctt ctcaaacggt gcgaacgtca cgctcgatat ctgggaaaac
     1081 ttgctctcat ccgccagaat gtaacagtct ctgcaattct ccatggattt tcgctttacc
     1141 atcgcctctt ttaactccgg tgtggaaaat cccctctgca ggctgacacc attggctccc
     1201 cagaagcctt tcgtaaaatt gtatttatcc agtgtaaaaa cggcttcctc accgacgata
     1261 gcctctgtca ccgccttgaa ctcgccgccc agaatataga ccttgcatcc atgctctgcc
     1321 agctttttcg cgtgaccgat ggcgttcgtc acaaacaccg cctgacggtt tgcaatgtaa
     1381 tcaatcatcc gctccgtcgt cgtacctgca tcgatataaa caaagtcttc ggcagtaatc
     1441 agtcctgccg catagcgtgc gatcttatcc tttgcctccc ggttctgctc cttgcggtaa
     1501 accacttcat catcgatggt actgtagacc gtattcttca aaatcgctcc gccgtgtacc
     1561 ttgacaagcc ggccgtttgc atccagcgta ttcaggtctc ttcgcacagt ggattcagaa
     1621 gcgtcaagtt ccgtcatcag ctgctgaacc gtcacactcc ccatatgttc cagtatggac
     1681 aaaatctttg caaatctttc ctctgtcagc atgatttatt ctccgttttc cgtcatattc
     1741 gttcaaaatc gttcatctct atagttgcat tatatttccg tttccagtca cagtcaatca
     1801 ttttccgtca aaatcgttca caaaaatcgt catcaaaatt tgtataaaac acacaaataa
     1861 aaagaaggct gacttcttca cgaaatcatc cttcctcaca aaattagtat ctcatattgc
     1921 cctcccgtag cggcggatcg cctcccggat ctcctcctcc gtcacccgca caagctcggt
     1981 gttgcgcgtc cggtactcgc gcatcacgtc aaacggacgg tttgccagat agcaccgcag
     2041 tgccatcagc accgcactgt gcgtcacgac aagcacatcg tccttctcgc gcgccagaat
     2101 ctgttccagc gcagccagcg tgcgccgcag cacctcgtta tagcactctc cgcccggcgt
     2161 acggcagaat ctcctgtggg tatcccagat atgatgccgt tctgcttccg tttcccttat
     2221 ctcatcccag ttcctgcctt cccagtctcc cagatccatc tctcgcagat cccagagccg
     2281 gatgcagtca atgcccagcg cctccgcgga aaaacgggct gtctctgccg cccgaagctg
     2341 cggcgacgtg tagactctgt ccagatgaag ctccccgttt cgctgcttct ctaagagtgt
     2401 ctctgcgagc cgcttcgcct gctgtattcc cttctcatcc agcggaatgt ccgtcgttcc
     2461 ctgtatcttc ttttttacat tccattccgt ctcgccatgg cgtgtaaagt aaaaat
//
LOCUS       DBMDQJ010000364         2512 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14655_length_2512_cov_1.677312, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000364 DBMDQJ010000000
VERSION     DBMDQJ010000364.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14655_length_2512_cov_1.677312"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(182..313)
                     /locus_tag="ACZO9R_07875"
     CDS             complement(182..313)
                     /locus_tag="ACZO9R_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913018.1"
                     /translation="MWKARGARRKGEKYNQEPFHIQHVLTVEPVHELTGLIWAAALQ"
     gene            complement(300..476)
                     /locus_tag="ACZO9R_07880"
     CDS             complement(300..476)
                     /locus_tag="ACZO9R_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015517652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate--pyruvate lyase"
                     /protein_id="HIR0913019.1"
                     /translation="METWYYEVVSIDGDYANLRRTDIPSEDLKLVARALLPAEIMEGS
                     RLKYELMQYEIVEG"
     gene            complement(524..907)
                     /locus_tag="ACZO9R_07885"
     CDS             complement(524..907)
                     /locus_tag="ACZO9R_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR0913020.1"
                     /translation="MFFIMGITDGRKDFDFHQMMTCGACGAYGRYQVFMTYTVLSIFF
                     IPCIKWNRHYYVQASCCNALYELDAEIGKRIARGGDVEILPQHLRPVHPGNRGNGWNT
                     TKQCRNCGFTTTEDYEFCPKCGLRF"
     gene            1359..2276
                     /locus_tag="ACZO9R_07890"
     CDS             1359..2276
                     /locus_tag="ACZO9R_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HIR0913021.1"
                     /translation="MEYVLLLVGFVLLVKGADFFVEGSSSLAKILKVPSVIIGLTIVA
                     MGTSAPEASVSINAALGGSNDIAISNVVGSNIFNGLVVVGICAFLSGFQTNAEILKRD
                     MPVNILVTLILCLMFLDGTLGRVEGFLLLAGMVLYITCMIRSALRNREEGEVCTVLPL
                     PKSILFMGGGLAAVIFGGDLVVDNACIIAANFGVSQNFIGLTIIAIGTSLPELVTSIV
                     ATRKGDSGLALGNAVGSNLFNILFILGMSAAISPLHVLGESVIDCVLLLCSGILLFVF
                     ARTKRSMSRAEGAVCVLLYVAYTAYLFVR"
BASE COUNT          534 a          670 c          570 g          738 t
ORIGIN      
        1 ggctttcttc caagatgctt ccaccgtatg catctctctc tgtagggtgc cgcactggta
       61 ctcttctctt cttcgcgctg ctttagattc tatttcaaaa aatcagaact gtcaaggtgt
      121 ttgcagcgaa tttccgaaaa caatctgctt tccccaccct gcatagtggc ttaccgtccg
      181 ctcattgcag cgccgccgcc cagatcagcc cggtcagctc gtgcaccggt tccaccgtca
      241 gcacatgctg aatgtgaaat ggctcctgat tgtatttttc tccttttcgc cgtgcccctc
      301 tagccttcca caatctcgta ctgcatcagc tcgtatttca atctgcttcc ttccataatc
      361 tccgccggaa gcagtgcccg cgccacaagc ttcaaatctt ctgacgggat atccgttctt
      421 cttaagttcg cgtagtctcc atcgatcgag accacttcat aataccatgt ctccattgtt
      481 ctctcctttg taacctcttt ctattccgca tcccgcgccg ctgttaaaac cgcagaccgc
      541 atttcggaca aaattcataa tcctccgtgg tcgtaaaacc gcaatttctg cactgttttg
      601 tagtattcca gccgtttcca cgattccccg gatgcaccgg acgcaaatgt tgtggcagaa
      661 tctcgacatc cccgccgcgc gcaatccgtt ttccaatctc cgcgtccagc tcatagagcg
      721 cattgcaaca gctcgcctgc acatagtagt gtctgttcca ttttatgcag gggataaaga
      781 aaatcgaaag tacggtgtat gtcatgaaca cctgatatct gccgtaagct ccgcaggcac
      841 cgcaggtcat catctggtga aaatcaaaat cttttctccc gtccgtaatt cccataataa
      901 aaaacatgtg tcctcacgct ccgttctctt gccgtttgtt atctgttatt atagacatat
      961 ccatcgattg ttacaacgat aatcttgcaa aaccgtccgg atgatatacc gcgctcattg
     1021 cgcctggatt tcatgtgggt tccacccggg ttccgcttgg atttcgttcg gatttcatgc
     1081 agattccgcc tggcttccgc ttggatttcg tttggatttc gacaaatagc gacattttta
     1141 ttgacatctt ttttgccccg tcctaaaata gccttaataa aagggagtag ttttcatctt
     1201 cggatgcgcc agacgctgcg cgcagaagct gaaataataa tcgtcatcac gccgcaaggc
     1261 cggttattat tgtaagtaac gagactttta tcactcattt ttgatgcgat aaaggtcttt
     1321 ttttataccc atttctttgt cgcaggaaag gaacacgtat ggaatatgta ttgttactcg
     1381 tcggttttgt actcttagtc aagggtgcgg atttctttgt ggagggaagt tcttcgctcg
     1441 ccaaaatctt aaaggtgccc agcgtcatca tcggtcttac gatcgttgcc atgggaacca
     1501 gcgctccgga ggcgtccgtc agcatcaacg ctgcgcttgg cggcagcaac gatattgcca
     1561 tcagcaatgt cgtcggatcg aatatattca acggtctggt tgtggtcgga atctgcgcct
     1621 ttttgtccgg ctttcagacg aacgcggaga tcttaaagcg ggacatgccc gtcaatattc
     1681 tggtcacgct gattctgtgc ctcatgtttc tcgatggaac gcttggccgc gtcgaaggtt
     1741 ttcttcttct ggcaggaatg gttctctaca tcacatgcat gatccgttcc gccctgcgca
     1801 accgtgaaga aggcgaggtc tgtacggtat tgccgcttcc gaaaagtatc ctctttatgg
     1861 gtggcggact tgccgctgtc atcttcggcg gcgatctggt cgtcgacaac gcctgcatca
     1921 tcgccgcaaa cttcggagtc agccagaatt ttatcggatt gaccatcatc gccatcggca
     1981 cttccctgcc ggaacttgtc acctcgattg tggcaaccag aaaaggagac agcggtctgg
     2041 cgctcggcaa cgcggtcgga tcgaatctct tcaatatcct gtttatcctt ggcatgtccg
     2101 ctgccatctc gccgcttcat gtgctcggcg aatcagtgat cgactgcgtc ctgctgctct
     2161 gtagcggtat cctgctgttt gttttcgcga gaaccaaacg gtccatgagc cgtgcggagg
     2221 gcgccgtctg tgtgttattg tatgttgcct acacggcata tctgtttgta agataagaac
     2281 accgtttcct cttactcgaa ggaattgtcg caataagcat atcgcgcttt gctttgataa
     2341 gtaatattat aagaaacgca cttggggatt tcatgtggaa atgcagatgg aatattcgtt
     2401 ggagaaaaat gtgtgcattt cttcctgctc aatgtttgca tcgaattccg tgtctcttcc
     2461 acgtttggac acctggtggg cgcatgtcct acaaattgtc tcgcggggtg cg
//
LOCUS       DBMDQJ010000365         2507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14686_length_2507_cov_1.628429, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000365 DBMDQJ010000000
VERSION     DBMDQJ010000365.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14686_length_2507_cov_1.628429"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="ACZO9R_07895"
     CDS             <1..642
                     /locus_tag="ACZO9R_07895"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR0913022.1"
                     /translation="YGKVEEVPQRETTPFHPYSPYAVAKQYGYWIVKEYREAYNMFCC
                     SGILFNHESERRGETFVTRKITLAAARIAQGKQDKLYLGNLSSLRDWGYAKDYVECMW
                     LILQHDKPEDFVIATGVQHSVREFCEYAFREAGIELEFKGEGMDEVGIDKATGKVVIE
                     VSKEFYRPTDVVNLWGDPTKAKSELGWNPTKTTFEELVKIMVKHDMELVAKEN"
     gene            738..1679
                     /locus_tag="ACZO9R_07900"
     CDS             738..1679
                     /locus_tag="ACZO9R_07900"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase family protein"
                     /protein_id="HIR0913023.1"
                     /translation="MEKDAKIYVAGHRGMVGSAIVRQLEKEGYHNIITRTHKELNLCR
                     QEDVEKFFAEEKPEYVFLAAAKVGGIMANSEALADFMYDNMTLEMNVIHEAWKNGCKK
                     LMFLGSSCIYPRMAPQPMKENCLLTGELEKTNEAYALAKISGLKYCEFLNKQYGTDYI
                     SVMPTNLYGPNDNYHPEHSHVLPALIRRFHEAKVSGAKEVTCWGTGTPLREFLYVDDL
                     ADACVFLMNNYSGDETVNLGTGKELTIKELTDLVAKVIGYTGEIKWDPSKPDGTPRKL
                     LDVSKLESLGWKYKTELEDGIRLAYEDFLNNPMRAER"
     gene            1708..>2507
                     /locus_tag="ACZO9R_07905"
     CDS             1708..>2507
                     /locus_tag="ACZO9R_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR0913024.1"
                     /translation="MYIVLLSGGSGKRLWPLSNDLCSKQYIKLMNHDEEQENREKCSM
                     LQRVFGQLKNVGLSENTIVCASSAQVELIESQLDGAAEVAVEPMRRDTFPAVMLSCAY
                     LLSKKGAKEDDVVAFLPVDPYTTGEFFRRIKLLGDKIAASEDTIGLLGGVPTYPSTKY
                     GYIVPEQGEGELLKVTGFREKPDEATAEKLVSDGALWNCGVFCFKLSMAAGWLAKYGV
                     GVDYDKMVADYEKLPKKSFDYEVLEHWERIVAIKYADLWKDLGTWNTLT"
BASE COUNT          679 a          558 c          741 g          529 t
ORIGIN      
        1 tacggaaagg tagaggaagt accgcagcgt gagaccacac cgttccatcc gtacagcccg
       61 tacgccgtag caaagcagta tggctactgg atcgtaaagg agtaccgtga ggcatacaac
      121 atgttctgct gcagcggtat cttattcaac catgagtccg agcgccgcgg agagactttc
      181 gttaccagaa agatcacact tgcagcggca agaatcgcac agggcaagca ggacaagctg
      241 tatctgggca atctgtcctc cctgcgtgac tggggatatg caaaggatta tgtagagtgc
      301 atgtggctga tcttacagca tgacaagccg gaggatttcg tgatcgcaac cggtgtacag
      361 cactccgtca gagagttctg cgagtatgcg ttccgcgagg cgggcatcga gctggaattc
      421 aagggagagg gcatggacga ggtcggtatc gataaggcaa ccggcaaggt agtcattgag
      481 gtatccaagg aattctatcg tccgaccgac gttgtcaacc tctggggaga tccgacaaag
      541 gcgaagtcag agcttggctg gaacccgaca aagacaacct tcgaggagct ggtgaagatc
      601 atggtaaaac atgacatgga gctcgtcgca aaagagaact aaataacagt ccgttcagat
      661 gttatgcctg gcgtgtcgca gcagtagtct ctgccgcggc acgccaggtt gtggtaaaaa
      721 aggagagaaa gatcatgatg gaaaaagatg cgaaaattta cgtggcagga cacagaggaa
      781 tggttggatc tgcgatcgtg cgtcagctgg aaaaagaagg atatcacaac attatcacaa
      841 gaacacataa agagttaaat ctctgccgtc aggaggatgt ggaaaaattc ttcgctgagg
      901 agaagccgga gtatgtattc ctcgcagcgg caaaagtcgg cggtatcatg gcgaacagcg
      961 aagcgctggc agattttatg tatgacaata tgacactgga gatgaatgtg attcatgagg
     1021 catggaagaa cggctgcaag aagctgatgt tccttggttc ctcctgcatc tatccgcgta
     1081 tggctccgca gccgatgaag gagaactgcc ttctgaccgg agagctggag aagacgaacg
     1141 aggcatatgc gctcgctaag atcagcggac tgaaatattg tgaatttttg aacaagcagt
     1201 atggcacgga ttacatttct gtcatgccga ccaacctcta cggaccgaac gataactacc
     1261 atccggagca cagtcatgtt cttccggcac tgatccgccg tttccacgag gcgaaggtgt
     1321 ccggtgcaaa ggaagtgacc tgctggggta ccggcacacc gctccgcgag ttcttatatg
     1381 tggatgatct ggcggatgcg tgcgtctttt taatgaacaa ttacagcgga gatgagaccg
     1441 tgaacctggg aaccggaaaa gagctcacga tcaaggaact cacggatctg gtcgcaaagg
     1501 tgatcggcta taccggagag atcaagtggg atccgagcaa gccggacgga acaccgagaa
     1561 agcttctcga tgtcagcaag ttggagagtc tcggctggaa atacaagacg gagcttgagg
     1621 acggcattcg tcttgcatat gaagatttct taaacaaccc gatgagagcg gaacgataga
     1681 atcaatttcg attaggagga actgtagatg tatattgtgt tattatccgg tggctcggga
     1741 aaacgcctgt ggccattatc caacgattta tgctccaagc agtatatcaa actgatgaat
     1801 cacgacgagg aacaggagaa cagggaaaaa tgctccatgc tgcagcgtgt gttcggtcag
     1861 cttaagaacg tgggactttc cgagaacacg atcgtgtgcg caagcagcgc gcaggtggaa
     1921 ctgatcgaga gccagctcga cggtgcggct gaggtagcag tggagccgat gcgccgcgat
     1981 acattcccgg cagtcatgct ttcctgtgca tacctgcttt ccaagaaagg tgccaaggaa
     2041 gatgatgtcg tggcattcct tccggtagat ccgtatacga ccggggaatt cttccgccgc
     2101 atcaagcttc tgggagataa gattgcagct tccgaggata cgatcggtct gctcggcggt
     2161 gtgccgactt acccgtctac caagtatggt tatatcgtgc cggagcaggg cgagggagaa
     2221 ctgttaaaag taaccggatt ccgcgagaag ccggacgagg cgacggcaga aaagctggtt
     2281 tccgacggtg cactgtggaa ctgcggcgtc ttctgcttca agctttccat ggcagccggc
     2341 tggttggcaa aatacggcgt cggcgtcgat tacgataaga tggttgccga ttatgagaag
     2401 cttccgaaga agagctttga ctatgaagtg ttagagcact gggagcgcat cgtggcgatc
     2461 aaatatgcgg atctgtggaa ggatctgggt acatggaaca cactgac
//
LOCUS       DBMDQJ010000366         2507 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14689_length_2507_cov_1.210723, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000366 DBMDQJ010000000
VERSION     DBMDQJ010000366.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14689_length_2507_cov_1.210723"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(193..1527)
                     /gene="tig"
                     /locus_tag="ACZO9R_07910"
     CDS             complement(193..1527)
                     /gene="tig"
                     /locus_tag="ACZO9R_07910"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HIR0913025.1"
                     /translation="MKKRTVALMLCAMMAFGAAGCGAKDADPAATEATESAASTEGLA
                     DSAVSGTITSTPCASSAFDLKGSDYVTLCDYSAIPVTITGDYDVDDQKVKDYFKQMFD
                     NYGPFYTADPDKTTVEEGDIVNVDYVGKLDGTAFSGGTAENQNIDVYQNASATGTGYI
                     DGFTDGLKGASVGDVIDCDVTFPDDYGNTDLAGKAVVFTFTVNSIQKEMTIDDVDDAF
                     AKEQFQVDTVDEMYGQIRSYMESSAEYNKQKDTTTAVQNYLIDNCEAEVPEDYLAARV
                     DDYRRQYIEQNCNGDESQLADYVSTYYGKTVEEMEAYWNEGMEKSIRLEFIMDAIADE
                     LGVEVNQDDIDAYAEQLVSQNGYESVEAMYNIYGFGDTDYGKRYFAALYRYDQALDQL
                     LETAVVTVDPSADTESDAVTEDAADEAEAGSATDASTTYEAAESTEETGAAN"
     gene            1720..2235
                     /locus_tag="ACZO9R_07915"
     CDS             1720..2235
                     /locus_tag="ACZO9R_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913026.1"
                     /translation="MSTVTIVLLVILAVLIVGLIVLYFLGKKAQKKRDEQEAQLAANS
                     QTVSMLIIDKKRLRLKDSGLPQVVIDQTPKMMRRSKLPIVKAKVGPKVMSLIADEAVY
                     DLIPLKKEVKATVSGIYITKVTGVRGPLEKPQGKKSFRARMQEKAKKANAELEKEKAE
                     LAKEKAAKKKK"
     gene            complement(2261..>2507)
                     /locus_tag="ACZO9R_07920"
     CDS             complement(2261..>2507)
                     /locus_tag="ACZO9R_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YwiB family protein"
                     /protein_id="HIR0913027.1"
                     /translation="TKRGETNVHMMFEKDKKNVTYYYTPYGNLLIGIDATKVQVEETE
                     HNLSVKVDYALEVNYEHMADCKITMNIQSKNAGDFKI"
BASE COUNT          590 a          716 c          523 g          678 t
ORIGIN      
        1 cccatccgcc gatggtcact gtcgtatcca gatactcatc cttgctgcga aataaatccc
       61 ttatatttac cagatccata cattcctcca tccgcaacag acgggtgcgc cactctgccg
      121 catcccgtct gccactctct atctcttttc tattatctta tctgcattgc cgcaaaaaat
      181 caatgcttat tcctaattgg ctgccccggt ctcttccgtg ctttctgccg cctcatacgt
      241 cgtagaggca tctgttgcac ttcctgcctc tgcttcatcc gcagcgtctt ccgtcaccgc
      301 gtcgctctcc gtgtccgcag acggatccac cgtcacaact gccgtctcaa gcagctgatc
      361 cagcgcctga tcgtagcggt agagagctgc aaaatagcgt ttgccataat cggtatcccc
      421 aaaaccgtag atattataca tcgcctccac agactcgtat ccgttctggc ttaccagctg
      481 ctctgcgtat gcatcgatgt cgtcctggtt cacctcaacg ccgagttcat ccgcgatcgc
      541 atccataatg aactcaaggc ggatgctctt ctccattccc tcattccagt atgcttccat
      601 ctcctcaacg gtctttccgt agtaggtgct cacataatcg gcaagctggc tctcatctcc
      661 gttgcagttc tgctcgatgt actgtcttct gtaatcgtcc actctcgccg caagataatc
      721 ctccggcacc tcggcctcgc agttgtcgat cagataattc tgcactgcgg tcgtcgtgtc
      781 tttctgcttg ttgtactctg cactgctctc catgtagctg cggatctgac cgtacatctc
      841 atccacggta tccacctgga actgctcctt ggcaaatgcg tcatccacat catcgatcgt
      901 catttccttc tgaatggagt tgaccgtaaa tgtaaagact actgcttttc ccgccagatc
      961 cgtgttgcca taatcatccg gaaatgtcac gtcacagtcg atcacatctc cgacggaagc
     1021 gccctttaag ccgtccgtaa atccgtcgat gtagcctgtc cctgtggcag atgcgttctg
     1081 gtacacatca atgttctgat tctcggcggt acctccgcta aacgctgttc cgtcaagctt
     1141 gccaacgtaa tccacattga caatgtctcc ctcctcgacc gtggtcttgt ccggatctgc
     1201 cgtatagaac ggaccatagt tgtcaaacat ctgcttgaaa taatccttta ccttctggtc
     1261 atccacgtca taatccccgg tgatcgtaac cggaatcgcg ctgtaatcac agagtgttac
     1321 ataatcgctt cccttcaggt caaacgcgct gctcgcacac ggagtgcttg tgatcgtacc
     1381 ggaaaccgca ctgtctgcaa gaccctcggt tgacgctgcg ctctctgtcg cctctgtcgc
     1441 tgccggatcg gcgtcctttg ctccgcatcc tgctgcacca aatgccatca tagcgcaaag
     1501 catcagtgct actgttctct tcttcatata caaataccct ttctattccc tcttttggcg
     1561 aattctgttt cgccaacact aaccttagta tcgtaccaca tttccctcaa aaaaggaagc
     1621 agacaagcca aactttcaca aatgtttcaa atacaagatt gcttttttgc cgaatcaatg
     1681 ctacaattaa atgcagtttg attgtaggag gattttacca tgagtaccgt aaccattgta
     1741 ttgctggtta ttcttgccgt cctcattgtc ggcttgatcg tattatattt tctcggcaaa
     1801 aaggcacaga aaaagagaga cgagcaggag gcgcagcttg cagccaactc ccagaccgtc
     1861 tccatgctga tcatcgataa aaagcgattg cgtctgaaag attcgggact tccgcaggtt
     1921 gtcatcgacc agacaccgaa gatgatgcgc cgttccaagc ttccgatcgt aaaggcaaag
     1981 gttggtccga aggtgatgtc actgatcgca gacgaggcag tctatgatct gattccgctg
     2041 aagaaggaag tcaaagcgac cgtgagcggc atctacatca cgaaggtaac cggcgtcaga
     2101 ggacctcttg agaagccgca gggcaagaag agtttccgcg cgagaatgca ggaaaaagcc
     2161 aagaaggcca atgcagagct ggaaaaagaa aaggcagaac ttgccaaaga gaaagccgca
     2221 aagaagaaaa aataagcagc tatttaaaat gcagggaatt tcagattttg aaatcccctg
     2281 cattttttga ttgaatgttc atggtaatct tgcagtccgc catgtgctca taattcactt
     2341 ccagcgcata atccactttc acggacagat tgtgttccgt ctcctccacc tgcactttcg
     2401 tggcgtcgat tccgatcagc agattcccgt acggcgtata ataataagtg acatttttct
     2461 tgtctttttc aaacatcata tgtacattcg tctcaccgcg cttcgtg
//
LOCUS       DBMDQJ010000367         2498 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14749_length_2498_cov_1.496037, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000367 DBMDQJ010000000
VERSION     DBMDQJ010000367.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14749_length_2498_cov_1.496037"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="ACZO9R_07925"
     CDS             <1..881
                     /locus_tag="ACZO9R_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR0913028.1"
                     /translation="ENWNDLYAYSKERHEENPAQYGYMKSLYDVYFSSGFLFSYGGYV
                     FGEENTNPEDVGFSAGEAEKGAWVLQQLAGVMNEECIDDTITTNAYSKLGDGTYFATL
                     STPDMYSTFADELEKEYEAEGMNEEEARETAKQNLIMTTLPKLPESGDLSESDPVLID
                     MKAMGGVNGYAVSAYTKAPNACLAFIDFATSYEMVTRRSEMLGIAPTRGDAAESAGDI
                     SEKIYANLENGNVVLMPSISEVSQIWTPAQTFFTDLAKDAFRSGSEKKYQDLPALKSG
                     LEQVDTQIHDAIYTLK"
     gene            901..2220
                     /locus_tag="ACZO9R_07930"
     CDS             901..2220
                     /locus_tag="ACZO9R_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0913029.1"
                     /translation="MKQKIKHAAAWFGDSSGNVKLSMVIMGAGQFRYGAKGKGVCFFL
                     AEVLMICYMAMRGCRDLAGFFTLGTTRGDAWLGVEGDNSVVMLLMGIFAWIVLAAFVC
                     IYCGNVRDAYRMQKRIEQGRSVLSFREEAAQLLDKKFYVTVLALPVIGVFIFQILPVL
                     FMILIAFTNYGGDIVPPELVDWVGLANFKKLLTLTQFAPTFFKILGWNVVWALVSTAL
                     NYFAGLGLALLLNKDCVRGKAIWRAFPVLAYAIPGFITLLAFKFMFSYGGPVNQIIVA
                     HGGSAVGFLDLDAKWTARLIGLLVNCWISTPQIMLLATGILSNRDASLYEAARIDGAG
                     RMQQFRKLTLPFVLFSTMPVLIGQFIGNFNNFGIFYFLRGGLYMDGYFLASDTDLLIN
                     WLYNLSIDNNYYCIGAAISLIIFFITSAISLAVYIKSPSYREEDTFQ"
     gene            2217..>2498
                     /locus_tag="ACZO9R_07935"
     CDS             2217..>2498
                     /locus_tag="ACZO9R_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR0913030.1"
                     /translation="MSRKTEKKRRMSAGRILDTGMTYLILLAVAFVFFFPCLWLILAS
                     FSKSGTIYSFDGFFPTGYSLASFKRLFTDTALYNYPRWFANTLFVAAGSC"
BASE COUNT          587 a          566 c          736 g          609 t
ORIGIN      
        1 tggagaactg gaacgatctg tatgcatatt ccaaagaacg ccatgaagaa aatccggcgc
       61 agtacgggta tatgaagtcg ctgtacgatg tgtatttttc cagcggattc ttattttcct
      121 acggcggcta tgtgttcggc gaggagaata cgaacccgga ggacgtcgga ttttctgccg
      181 gcgaggcgga aaaaggcgcg tgggttctgc agcagcttgc gggtgtgatg aacgaggaat
      241 gtatcgacga cacgatcaca accaacgcat acagcaaact gggggatggc acctattttg
      301 cgacgctctc gacaccggat atgtactcca cgttcgcaga tgaactggaa aaagaatacg
      361 aggcggaggg catgaacgag gaggaagcca gggagacagc aaagcagaat ctgatcatga
      421 cgacactgcc gaagcttccg gaaagcggcg atctgtcgga gagcgatccg gtactgatcg
      481 atatgaaagc gatgggcgga gtcaacggat acgcagtgag cgcgtacacc aaggcgccga
      541 atgcctgcct ggcatttatt gattttgcga caagctatga gatggtcacc agacggagcg
      601 agatgctcgg aattgcaccg acgcgcggcg atgcggcgga gagtgcgggg gacatatcgg
      661 agaaaatcta tgcaaatctc gagaacggaa atgtggtgct gatgccgtcc atctccgagg
      721 tgtcgcagat ctggacgccg gcacagacct tttttacaga tttagcgaag gacgcgttcc
      781 gaagcggcag tgagaaaaag tatcaggatc tgcctgcgct aaagagcggg ctggaacagg
      841 tggatacaca gatccatgac gcaatatata cgttaaagta gcagatatgg agagaacggt
      901 atgaagcaaa aaataaagca tgctgctgcc tggtttggtg acagcagcgg gaacgtaaaa
      961 ttatcaatgg tcatcatggg ggcagggcag ttccgctatg gcgcaaaggg aaaaggcgtg
     1021 tgctttttcc tggcggaagt cctgatgatc tgctatatgg caatgcgcgg gtgtcgtgat
     1081 ctcgccggtt tttttacact gggtacgacc agaggggatg catggcttgg cgtagaggga
     1141 gataactcgg ttgtcatgct gttgatggga atatttgcgt ggatcgtcct ggcggcgttt
     1201 gtgtgtatct actgcggaaa cgtgcgcgac gcttaccgga tgcagaagcg gatcgagcag
     1261 ggaagaagcg tgttgtcctt ccgcgaggag gcggcacagc tgctcgacaa aaagttctat
     1321 gtcacggtgc tcgcgctgcc ggtcatcggt gtatttatct ttcagatcct gccggttctg
     1381 ttcatgatac tcattgcatt tacgaactac ggtggcgaca ttgtaccccc ggaactcgtg
     1441 gactgggtgg gacttgcaaa ttttaagaaa ctgctgacgc tgacgcagtt tgcaccgacc
     1501 ttttttaaga ttctgggatg gaatgtcgtg tgggcgcttg tctccactgc actcaattat
     1561 tttgccggtc tcgggctggc gctgctcctg aataaggact gcgtcagggg aaaagccatc
     1621 tggcgcgcgt ttccggtgct ggcatacgca attccgggat ttatcaccct gctggcgttt
     1681 aagttcatgt tttcctacgg cggaccggtc aatcagatca ttgtggcgca cgggggcagt
     1741 gcggtcggct ttctggatct cgatgccaag tggacggcgc gcctgatcgg acttctggtc
     1801 aactgctgga tcagtacacc gcagatcatg ctgctcgcca caggaatcct gtcaaaccgg
     1861 gatgcttcct tatatgaagc agcgcgcatt gacggggcgg gacgcatgca gcagttccgg
     1921 aaattgacgc tgccgttcgt acttttttcg acaatgccgg tgctgatcgg acagtttatc
     1981 ggaaatttca ataatttcgg gatcttttac ttcctgcggg gcgggctgta tatggacggc
     2041 tatttccttg cgagcgatac ggatcttctg attaactggc tctataacct gtccatcgac
     2101 aacaattatt actgcatcgg cgcggcgatc agtctgatta ttttctttat cacatcggca
     2161 atatcacttg cggtttacat aaaatcaccg tcttacaggg aggaggatac gtttcaatga
     2221 gcagaaaaac agagaaaaag cggcggatgt ccgcaggaag aatcctcgat accggaatga
     2281 cctatctcat attgcttgca gtggcgtttg tattcttctt tccgtgcctg tggctgatcc
     2341 tggcatcgtt ttccaaatcg ggaacaatct attcgtttga cggttttttt ccgaccggat
     2401 acagtctggc gagtttcaaa cggctgttta cggatacggc gctgtacaac tatccgcgat
     2461 ggtttgccaa tacgcttttt gtggcggcag gcagctgc
//
LOCUS       DBMDQJ010000368         2497 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14758_length_2497_cov_1.524207, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000368 DBMDQJ010000000
VERSION     DBMDQJ010000368.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14758_length_2497_cov_1.524207"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            61..1326
                     /locus_tag="ACZO9R_07940"
     CDS             61..1326
                     /locus_tag="ACZO9R_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR0913031.1"
                     /translation="MEASVITLLFLLFAVVMFVWEKIPLGVTSMVVCIGLVVTGVLDW
                     KTAFAGFVDSNVILFVAMFIVGGALFETGMANKIGGVVTHFAKSEKQLIIAIMIITGV
                     MSGVLSNTGTAAVLIPVVIGIAAKSGYSRSRLLMPLVFAAAMGGNLSLIGAPGNLIAQ
                     SAMEDMGKAFGFFEYAKVGLPILITGTIFFALFGYKLLPEHKGGEDGIYDETKDFDHV
                     PKWKQNLSLIILVLTLIGMIFEKQIGIKLCVIGAVGALALILTGVISEKDALKAIDLK
                     TIFLFGGTLSLASALEETGAGEMIADKVIGLLGANPSPYLLTFVVFMLCCVLTNFMSN
                     TATTALMVPICVSIANRMGADPSAVLMACVIGGSCAYATPIGMPANTMVVTAGGYTFK
                     DYAKAGIPLILVATVVSMILLPVLYPFFP"
     gene            1390..2289
                     /gene="ttdA"
                     /locus_tag="ACZO9R_07945"
     CDS             1390..2289
                     /gene="ttdA"
                     /locus_tag="ACZO9R_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HIR0913032.1"
                     /translation="MSKEAQVEQLTDYMAKFIAHVAKKLPDDVIAKLTELRAQEDSPL
                     SKTIYDTMFQNQELAVKLNRPSCQDTGVLQFWVKCGTNFPLIGELEALLKEAVVKATF
                     EAPLRHNSVETFDEYNTGKNVGKGTPTVFWDIVPDSDQCEIYTYMAGGGCTLPGKAMV
                     LMPGEGYEGVTKFVLDVMTSYGINACPPLLVGVGVATSVETAALLSKKALMRPIGSHN
                     DNERAAKMERLLEDGINAIGLGPQGMGGKYSVMGVNIENTARHPSTIGVAVNVGCWSH
                     RRGHIIFDKDLNYTITTHSGVEL"
     gene            2289..>2497
                     /locus_tag="ACZO9R_07950"
     CDS             2289..>2497
                     /locus_tag="ACZO9R_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase C-terminal domain-containing
                     protein"
                     /protein_id="HIR0913033.1"
                     /translation="MVEMKDGKKILTTPISAEDLADIKAGDIIYLNGSMTTCRDVAHR
                     RLVEEGRELPVDVRNNAIFHAGPIIR"
BASE COUNT          633 a          549 c          688 g          627 t
ORIGIN      
        1 tgatgggaag ggtttttaca tggcggttgc attttgcttc gcaaaacaaa ggagggtttt
       61 atggaagcat cagtaataac actattattt ttgttgtttg ccgtcgtgat gtttgtctgg
      121 gagaagattc cgctcggcgt aacatccatg gtcgtctgta tcggtctcgt cgtcaccggc
      181 gtgcttgact ggaagacggc gtttgcagga tttgtggaca gcaatgtcat cctgtttgtg
      241 gcgatgttca ttgtcggggg tgcgctgttt gagacaggga tggcaaataa aatcggaggc
      301 gtggtcacac acttcgccaa atcagagaaa cagctgatta tcgccattat gatcatcacc
      361 ggtgtgatga gtggcgtact ttcaaatacc ggtaccgcag cagtgctgat cccggtagtc
      421 atcggaatcg ccgcaaaatc cggttattcc agatcaagac ttctgatgcc gcttgtattt
      481 gcggcggcta tgggaggaaa cctttctctc atcggagcgc cgggcaacct catcgcccag
      541 tcggcgatgg aagacatggg gaaagcattt ggatttttcg agtatgccaa agtcggtctg
      601 ccgatcctga ttacaggaac tattttcttt gcactgtttg gctacaagct tctgccggag
      661 cacaagggcg gagaggatgg aatctatgat gagacaaagg atttcgacca tgtaccgaag
      721 tggaagcaga atctttccct catcatactg gtactgacac tgatcggaat gatttttgaa
      781 aaacagatcg gcatcaagct ctgtgtgatt ggtgcggtcg gcgcactggc attgatctta
      841 accggtgtca tcagtgagaa agatgcctta aaggccatcg acttaaagac aatcttcctc
      901 ttcggaggta cgctttccct ggcgtcagcg cttgaggaga caggtgcagg tgagatgatt
      961 gcggacaagg tcattggtct tctgggagcg aacccgtcac cgtatcttct gaccttcgtg
     1021 gtattcatgc tctgctgtgt actgaccaac tttatgtcga acacggcaac gactgcactt
     1081 atggttccga tctgtgtatc cattgcgaac cgtatgggag ctgatccaag tgctgttctg
     1141 atggcttgtg tgatcggcgg ttcatgtgcc tatgcgacac cgatcggtat gccggcgaac
     1201 accatggtcg ttactgccgg aggttatacc tttaaagatt acgcaaaagc gggaattccg
     1261 ctgattctgg tggcaaccgt tgtcagcatg atcctgcttc cggtattgta tccgtttttc
     1321 ccgtaaaatc agtttttgcc gcacagctgc ggtgtactgg atagttatag ataaaaggag
     1381 gacaaaaaaa tgtcaaaaga agcacaggtt gagcagctta ccgattacat ggcaaaattt
     1441 attgcacatg tggcaaagaa gctgccggat gatgtcattg caaaattaac agagctgaga
     1501 gcacaggagg acagcccgct gtccaagacg atctacgata ccatgttcca gaatcaggaa
     1561 cttgcggtaa agctgaatcg tccgagctgc caggataccg gcgtgctgca attctgggta
     1621 aaatgcggca caaactttcc gctgatcgga gaactggagg cactcttaaa agaagccgtt
     1681 gtaaaagcaa cgtttgaggc tccgctccgc cacaacagtg ttgagacatt cgatgagtac
     1741 aacacaggca aaaatgtcgg aaaaggaaca ccgacggtct tctgggatat tgttccggac
     1801 agtgatcagt gtgagattta cacctacatg gcaggcggcg gatgtacgct tccgggcaag
     1861 gcgatggtct taatgccggg cgaaggttat gagggtgtta ccaagttcgt actcgatgtc
     1921 atgacttcct atggaatcaa tgcatgtcct ccgcttcttg tgggcgttgg cgtagcaacc
     1981 tccgtggaga cagcggcact gctttccaag aaagcgctga tgcgtccgat cggttcccac
     2041 aatgacaatg agcgcgcggc aaaaatggag cgtctgcttg aagatggaat caatgcaatc
     2101 ggacttggac cgcagggcat gggtggcaaa tattccgtta tgggcgttaa catcgagaac
     2161 acggcgcgtc atccgtccac gatcggtgtg gcagtcaatg tcggctgctg gagccacaga
     2221 agaggacaca ttatctttga taaggatctg aactatacga tcacaacaca ttcgggggtg
     2281 gaattataat ggtagagatg aaagatggaa agaaaatttt aacaactccg atttctgcgg
     2341 aggatcttgc agatattaag gccggagata ttatttatct gaacggaagc atgacaacct
     2401 gccgcgatgt tgcacaccgc cgtctggttg aggagggaag agaacttccg gtagatgtca
     2461 gaaacaacgc gattttccat gcaggaccga tcatccg
//
LOCUS       DBMDQJ010000369         2493 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14784_length_2493_cov_1.489967, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000369 DBMDQJ010000000
VERSION     DBMDQJ010000369.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14784_length_2493_cov_1.489967"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="ACZO9R_07955"
     CDS             <1..362
                     /locus_tag="ACZO9R_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR0913034.1"
                     /translation="SIVGFFIGMLLQVFLFALKRHKNSKFITNILGSAFVTLLSLIVL
                     SFGTPILYDKVIIGDIMPLVPGIALTTSIRDFFNGDYLSGAIHMIDAILTAFCIAVGV
                     GTVITIYHFAMGGAALL"
     gene            359..811
                     /locus_tag="ACZO9R_07960"
     CDS             359..811
                     /locus_tag="ACZO9R_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR0913035.1"
                     /translation="MIIQFIVSLFATLSFAELFGAPKKELFFCGLTGAIGWVVYVIGL
                     NSNMGTVLANLTATFALTVFSRILSAIRRNPVTVYLIAGIFPLVPGAGIYYTSYYFIM
                     NDMTQFSNYGMETIKVAGAIVLGIVFGFGLPQSWFNALQRIERKKTAL"
     gene            complement(830..>2493)
                     /locus_tag="ACZO9R_07965"
     CDS             complement(830..>2493)
                     /locus_tag="ACZO9R_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HIR0913036.1"
                     /translation="GRTERTDADAHAQEQKWSLESDAKSMMTRLGITDFTQKTGELSG
                     GQRKRLALVAALLVPCDVLILDEPTNHLDSAMADWLENYLKKWRGALVMITHDRYFLD
                     SVCNRIVEVDKGAIYSYETNYSGYLERKAEREESAEASERKRQSVLRKELEWVRRGAK
                     ARTTKQKGRLQRYEELKNQKAPERDSQVEMSSVYSRMGKTTIELDHITKGYDGRTLLR
                     DFSYIFLKGDRIGFIGANGSGKTTLMKMIAGRIKPDAGTITVGATIKIGYYTQEIETG
                     REAGIAYMDPEEKVIDYIRNTAEYVRTTDGLVSASNMLERFLFPAAQQYSPIGKLSGG
                     ERRRLNLLRVLMEAPNVLILDEPTNDLDTQTLAILEDYLDSYEGIVIVVSHDRYFLDR
                     VVRRIFAFEGNGEIRQYEGGYTDYVNRLAEEGRKPGETAEVQGMTARGVSEAESAQEA
                     GGVTGTSGQDAAEQADSRNTWKREKKLKFSYKEQREYETIEDDIANLEAKVEQLDADM
                     AENATNSVKLGELLAEKEAAEQALEEKMERWEYLEELAAKIAGQE"
BASE COUNT          465 a          781 c          556 g          691 t
ORIGIN      
        1 acagcatcgt cggctttttt atcggcatgc tgctgcaggt atttctgttt gccttaaagc
       61 gccataagaa ttccaagttt atcacgaata tcctgggaag tgcattcgta acgctgctgt
      121 cgctgattgt actctccttt ggtaccccca tcctctacga taaggtcatc atcggagaca
      181 tcatgccgct cgtccccgga atcgcgctca ctacgtcgat ccgcgacttt ttcaacggag
      241 attacctctc cggtgccatt catatgatcg atgccattct gaccgcattc tgtatcgccg
      301 tcggtgtcgg caccgtcatt acgatctatc attttgctat gggaggggct gcgctgttat
      361 gattatccag tttattgtga gtttgtttgc cacactctct tttgcggaac tgtttggcgc
      421 gccgaaaaag gaactgtttt tctgcggtct gaccggtgcc atcggctggg tcgtctatgt
      481 gatcggctta aacagcaata tgggcacggt gcttgcgaat ctgaccgcca ccttcgcact
      541 caccgtattt tcgcgtattc tgtccgcgat ccgcagaaat ccggtaaccg tttacctcat
      601 cgccggtatt tttccgctgg ttcccggagc aggtatttac tatacgtcct actatttcat
      661 catgaacgat atgacgcaat tctccaacta tggcatggaa accatcaagg tggcaggtgc
      721 catcgtgctg ggaattgtct ttggcttcgg tctgccgcag agctggttta acgcactgca
      781 gcgaatagaa agaaaaaaga cggctttgta aaatgaaacg ttatattctt cattcctgcc
      841 ccgcaatctt cgccgccagc tcctccaggt actcccagcg ctccatcttt tcctccagtg
      901 cctgttctgc cgcttccttt tccgccagaa gttccccaag cttcaccgag ttcgtggcgt
      961 tttccgccat gtcggcatcc agctgctcca cttttgcttc caggttcgca atgtcatcct
     1021 cgatcgtctc gtattccctc tgttccttat aactgaattt cagcttcttc tcacgtttcc
     1081 aggtgtttct ggaatccgcc tgctctgctg catcctgccc ggatgtcccg gtcacaccgc
     1141 cggcctcctg tgcgctttcc gcctccgaga ctccgcgtgc tgtcattccc tgaacttccg
     1201 ccgtttcgcc cggctttctg ccttcctccg ccagtcggtt gacataatct gtataaccgc
     1261 cttcatactg ccggatctct ccgtttccct cgaaagcaaa aatacgccgc accacgcggt
     1321 cgaggaaata ccggtcatgc gataccacaa tcacgattcc ctcatagctg tccaggtagt
     1381 cttccagaat cgcaagggtc tgcgtatcca gatcgttcgt cggctcgtct aaaatcagta
     1441 cattcggcgc ctccatcagc acgcgcagca gatttaatct gcgccgctca ccgccggaaa
     1501 gctttccgat cggactgtac tgctgcgctg ccgggaacaa aaaacgctca agcatgttcg
     1561 atgccgagac cagaccgtcg gtcgtccgca catactccgc cgtgttacgg atataatcaa
     1621 tcactttttc ctccgggtcc atgtaggcga ttcccgcttc gcggcccgtc tcaatctcct
     1681 gcgtatagta gccgatcttg atcgttgccc caactgtgat cgtgcccgca tccggcttga
     1741 tgcgccccgc aatcatcttc atcagggtcg tcttgccgct tccgtttgcc ccgatgaatc
     1801 cgatccggtc ccctttcaga aaaatatagg aaaagtcccg cagcagcgtc cgcccgtcat
     1861 atcccttggt aatgtgatcc agctcgatcg tggtttttcc cattctggaa tagacggaac
     1921 tcatctccac ctggctgtcg cgctccggcg ccttctgatt cttgagttcc tcatagcgct
     1981 gcaagcgccc cttctgcttg gttgtgcgcg cttttgcccc ccgtctgacc cattccagtt
     2041 ccttccgcag tacagactga cgcttgcgct cactcgcctc ggcgctctcc tcccgctccg
     2101 ccttccgctc cagatagccg gaataattcg tctcatagct gtagattgcc cccttgtcca
     2161 cctcgacaat acggttgcag acgctgtcaa gaaaatagcg gtcatgcgtg atcatcacca
     2221 gcgcaccgcg ccattttttc agataatttt ccagccagtc cgccattgcc gaatccagat
     2281 ggttcgtcgg ctcgtccaga atcagcacat cacacggcac taaaagtgct gccacaagtg
     2341 ccagacgttt tctctgtcca ccggaaagtt ctccggtttt ctgtgtaaaa tcggtaatgc
     2401 cgaggcgcgt catcatgctt tttgcatcgc tctcaaggct ccatttctgt tcctgtgcat
     2461 gcgcgtctgc atcggtgcgc tctgtcctcc cgc
//
LOCUS       DBMDQJ010000370         2489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14812_length_2489_cov_2.087102, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000370 DBMDQJ010000000
VERSION     DBMDQJ010000370.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14812_length_2489_cov_2.087102"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(62..577)
                     /gene="bilS"
                     /locus_tag="ACZO9R_07970"
     CDS             complement(62..577)
                     /gene="bilS"
                     /locus_tag="ACZO9R_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein BilS"
                     /protein_id="HIR0913037.1"
                     /translation="MSGKKIAIIYKSLTGNTRQVAEAIRDALGGEEIVYFGEPKPDIV
                     ADLYFVGSWTDKGSCDGTVGEYLKQLEGKKLAVFGTAGYGGSREYYQKLACRVTECVP
                     DTSEVLGSFYCQGKMPIGVRNRYVAMLREHPEDQKLNASVKNFDEALSHPDEKDFADA
                     RQWAQTMVDAV"
     gene            complement(614..883)
                     /locus_tag="ACZO9R_07975"
     CDS             complement(614..883)
                     /locus_tag="ACZO9R_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913038.1"
                     /translation="MKKYVKPEVVETKEYAEGVYLASGGSTDNSSGPEETVCRFGRKE
                     ANAGSDTCQECSKSGGQRNTPLPGEESAREADFTGCIDGRPVKRS"
     gene            complement(1038..1727)
                     /locus_tag="ACZO9R_07980"
     CDS             complement(1038..1727)
                     /locus_tag="ACZO9R_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0913039.1"
                     /translation="MSRVLIIEDEVEIADLEKDYLELSGFEVEIENDGTVGLKRALTE
                     DFDMYILDLMLPGIDGFEICKQIREEKNTPILMVSAKKDDIDKIRGLGLGADDYITKP
                     FSPSELVARVKAHLARYERLIGSNTPENDTIEIRGIRIDKTARRVWVNGEEKQFTTKE
                     FDLLTFLAENPNHVFTKEELFRQIWDMESIGDIATVTVHIKKIREKIEMNTNKPQYIE
                     TIWGVGYRFKL"
     gene            complement(1705..>2489)
                     /locus_tag="ACZO9R_07985"
     CDS             complement(1705..>2489)
                     /locus_tag="ACZO9R_07985"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016439594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0913040.1"
                     /translation="GLEKDYKKAVKDNAEEKLSGERENKALISNIAHDLKTPITAVKG
                     YAEGLIDGVADTPEKRDKYIRTIYNKANEMDTLINELTLYAKIDTNRIPYNFAKLNVA
                     EYFNDCIEEIGLDLEAKNIGLAYFNYADEDVQIIADPEQLKRVVNNIVGNSVKYLDKQ
                     KGFINIRIKDVGDFIQVEIEDNGRGIAARDLPYIFDRFYRADASRNSATGGSGIGLSI
                     VKKIIEDHGGKIWATSKESIGTVMYFVLRKYQEVPYEQSIDH"
BASE COUNT          527 a          781 c          464 g          717 t
ORIGIN      
        1 ctgccggctt ttcgcagaca ataatctgct cgtcttcaaa taaaattctg atctccatat
       61 tctaaaccgc atccaccata gtctgtgccc actggcgtgc atccgcaaaa tctttctcat
      121 ccggatgtga aagcgcctcg tcaaaatttt ttacgctcgc attcagcttc tgatcttctg
      181 ggtgctcccg cagcatcgcc acatagcggt ttctcactcc gatcggcatt ttcccctggc
      241 agtaaaagct tccgagcacc tcactggtat ccggaacgca ctccgttacc cggcacgcca
      301 gcttctgata gtattctctg gagccaccat aaccggcagt gccgaaaacc gccagttttt
      361 ttccttccag ctgcttcaaa tactcaccaa ccgttccatc acagcttccc ttgtccgtcc
      421 aggatcctac aaaataaaga tccgccacaa tgtccggctt cggctcaccg aagtatacaa
      481 tctcttcacc gccaagcgca tcccgtattg cttctgccac ctgtctggta ttccctgtca
      541 gacttttgta tatgattgca atttttttcc cactcatctg tgctctcctt cgcccgctct
      601 gtcatcctac tgcctatgat ctctttaccg gtctgccgtc gatacatccg gtaaaatctg
      661 cttcgcgcgc agactcctcg cccggaagcg gcgtatttct ctgcccgccg ctcttagagc
      721 actcttggca ggtgtccgaa cccgcgtttg cttcctttct gccaaatcga cagacagttt
      781 cttccggtcc cgacgaatta tctgtcgaac cgccgctcgc cagatatacg ccctccgcat
      841 actcttttgt ctccacaact tctggtttta catacttttt cattttcctg cactccttcc
      901 aaattataaa ttacagctta aaccggtacc ctactcccca gattgttgct gcttcgcagc
      961 gcaggcgctc atttcagtac agcttcgctg tggagcgcct gccttgcaca cctttacaca
     1021 tccttacgat ttaagctcta cagcttaaat ctgtacccta ctccccagat tgtttctatg
     1081 tactgcggtt tgttggtgtt catctcgatc ttttcgcgga tctttttgat gtgtaccgtc
     1141 accgtcgcaa tgtccccgat ggattccata tcccagatct ggcggaacag ttcttccttc
     1201 gtaaatacat gattcggatt ctccgcaaga aatgtcagga ggtcaaactc ctttgtcgtg
     1261 aactgcttct cctcgccgtt gacccagaca cgtctcgccg tcttatcgat gcggattcca
     1321 cggatctcga tcgtatcatt ctcaggcgta ttgctgccga tcagccgctc atagcgcgcc
     1381 agatgtgcct tcacgcgcgc caccagctcg ctcggcgaaa atggcttggt aatatagtca
     1441 tccgctccca gtccaagacc gcggatttta tcgatgtcat ccttcttcgc cgacaccatc
     1501 agaatcggcg tgtttttctc ctcccggatc tgcttgcaga tctcaaaacc gtcaatgccc
     1561 ggaagcatca gatccaggat gtacatatca aaatcttccg tcaacgcccg tttcagtccg
     1621 acggttccgt cattctctat ctccacctca aatccgctga gttccagata atccttctca
     1681 aggtctgcaa tctccacctc atcctcaatg atcaatactc tgctcatatg gcacctcctg
     1741 atattttcgt aatacaaaat acatcacggt tccgatggac tccttgcttg tcgcccagat
     1801 cttaccgccg tggtcttcaa taatcttctt cacaatcgat aatccgatac cgctgcctcc
     1861 cgtcgcagaa ttgcgcgacg catccgcacg gtaaaaccgg tcaaaaatgt aaggcagatc
     1921 gcgcgcggca attcctctgc cgttatcctc aatctcgacc tggataaaat caccgacatc
     1981 cttgatccgg atattgataa atcccttctg cttatcgaga tatttgaccg aattccccac
     2041 gatattgtta accacgcgct ttagctgctc tggatccgca ataatctgga catcctcgtc
     2101 cgcataatta aaatatgcca gtccgatgtt tttcgcctcg agatcgagtc cgatctcctc
     2161 aatgcagtca ttgaaatact ccgccacatt cagctttgca aaattgtacg gaatgcggtt
     2221 ggtatcgatc ttcgcataca aggtcagctc attgatgagc gtatccatct cattcgcctt
     2281 attataaatc gtccggatgt acttgtcacg cttttccggc gtatccgcca caccgtcgat
     2341 gagaccctcc gcatacccct tcacggctgt gatcggcgtc tttaaatcat gggcgatatt
     2401 actgatcagc gccttgttct cacgctcacc ggaaagcttt tcctctgcgt tatccttcac
     2461 cgccttcttg taatccttct ccagccccg
//
LOCUS       DBMDQJ010000371         2489 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_14813_length_2489_cov_1.731575, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000371 DBMDQJ010000000
VERSION     DBMDQJ010000371.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14813_length_2489_cov_1.731575"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>2489
                     /locus_tag="ACZO9R_07990"
     CDS             <1..>2489
                     /locus_tag="ACZO9R_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR0913041.1"
                     /translation="IEQQANAQLATINESIAAWGQQLESLGIKVQTLDDLPYAIADMT
                     QKLAEINTGIATMQEAQKQVDAGKIALDEASAQLTKNQIEGILQLSEATAQLADAKNQ
                     LAQGQSQLDAAKDSAKESADLDQILTVDMLGNLLAAQNFSMPAGYVGEGENRYMVRVG
                     DKLDSVDELKQVVLIDLGLDGIGPVCLSDVAAVELVNNAGESYSKVNGNPAIMLSIEK
                     QTGYSTGDVTKRIQQKLKAMEADNPALHTAILMNQGIYIDIIVQSVLENMIVGALLSV
                     IVLILFLKDIRPTLVIACSIPLSVIFAVVLMYFSNISLNIISLSGLALGIGMLVDNSI
                     VVIENIYRLRSEGYSIKKAAVEGAGGVTGAIIASTLTTVCVFVPIVFTEGITRQLFVD
                     IALTITFTLTASLIVALTFVPMMAAGLLKNPKEVRHAFFEKVQQGYAKLLELALRFKP
                     VVFIGVIVLLVTSALAAYSRGFSFMDMNMETDQLTVTVAAKDDEKLTFEELCERADEV
                     TEKIAGIDGVETIGAIAGGDSALMSMGGSSDSVTMYLLLEENSSVTAGEITAQIAELT
                     KDMDCQVDTDNSASDMSSFLGSGITVQIKGSDLGTLQELAGKVTGVVEAQEGTVDVDD
                     GLDNTTQSYTIHVDKEKAAEYGMTTAQVFQLVYAKLASQTAVTTISVGVQDYEVYVRS
                     EEQANVTLDDIKNLTFSYEDKMAQLSGTGSDEEKETDEKLSEDIKDTGDAKDIEDTED
                     TGDTGDTVKEIPLTEIATFEAGSSLNAVSRDSQTRYISVTAGVDDTHNVTLVANALMK
                     ELNKIDLPEGYSIEMTGEDEMIADAMQQLYL"
BASE COUNT          651 a          542 c          767 g          529 t
ORIGIN      
        1 atcgagcagc aggcaaatgc acagcttgca acaatcaatg aatcgatcgc cgcatgggga
       61 cagcagctgg agagccttgg catcaaagta cagacactcg atgatctgcc ctatgcaatc
      121 gcagatatga cgcagaagct tgccgagatc aatacgggaa ttgcgaccat gcaggaggcg
      181 caaaaacagg tggacgcagg taaaattgcg ctggatgaag cctccgcgca gctgacgaag
      241 aaccagattg agggaatcct gcagttgagc gaggcgacag cgcagcttgc cgatgcaaag
      301 aatcagctgg cgcagggaca gagccagctg gatgcggcaa aggatagcgc aaaggaatct
      361 gcggatctgg atcagattct cacggtcgac atgctcggta acctgctcgc ggcacagaat
      421 ttttccatgc cggcaggcta tgtcggggaa ggcgagaacc gctacatggt gcgtgtgggc
      481 gataagctgg attccgtgga cgaattaaag caggtggttt tgattgatct tggtctggac
      541 ggaatcgggc cagtctgcct tagcgatgtg gcggcagtgg aactggtgaa caacgccggt
      601 gagtcttatt ccaaggtaaa cggaaacccc gcaatcatgc tttccattga aaagcagacc
      661 gggtactcga ccggggatgt gacaaagcgg atccagcaga aattaaaggc gatggaggca
      721 gataatcccg cacttcacac ggcgatcttg atgaatcagg gaatttatat cgatatcatt
      781 gtgcagtcgg tattggaaaa tatgatcgtg ggcgcgctgc tgtccgtcat cgtgctgatt
      841 ctgtttttaa aagacatccg gccgacgctc gtgattgcat gctccatccc gctcagtgtt
      901 atttttgcgg tggtgttgat gtatttttca aatatttcct taaacattat ctcgttgtcg
      961 ggactggcgc ttggcatcgg tatgcttgtg gataactcta tcgtggtcat tgagaacatc
     1021 tatcgcctgc ggagcgaggg atattccatc aaaaaggcgg cggttgaggg agccggcggc
     1081 gtgaccggag ccattattgc atcgacctta acgacggtgt gtgtgttcgt tccgattgtg
     1141 ttcacagagg gaatcacgag gcagctgttt gtggacatcg cactcacaat tacgtttaca
     1201 ttgacagcca gcctcatcgt ggcgctgacg ttcgtgccga tgatggcggc gggactactt
     1261 aaaaatccca aagaggtgcg gcacgccttc tttgaaaaag tgcagcaggg atatgcgaag
     1321 cttttggaac tggcgctcag gttcaagccg gtggtattca tcggtgtgat cgtcctgctt
     1381 gtgacgagtg cgcttgccgc gtattccaga ggattttctt tcatggatat gaatatggaa
     1441 accgatcagt tgaccgtgac tgtggcggcg aaagacgacg aaaaactgac gtttgaggag
     1501 ctctgtgagc gcgcagatga ggtgacagag aagattgcgg gcattgacgg cgtggagacg
     1561 atcggagcga tcgccggagg agacagcgcc ctgatgtcca tgggcggcag cagtgacagt
     1621 gtgacgatgt atctgcttct ggaggagaat tcctccgtca cggcgggaga gatcacggcg
     1681 cagattgcgg aactgaccaa agatatggac tgtcaggttg acacggacaa ttcggcgtcg
     1741 gatatgagtt ccttcctcgg aagcggtatt acagtgcaga tcaagggaag tgaccttggc
     1801 accctccagg aactggcagg aaaagtgacc ggtgtggtgg aagcacagga aggaacggtg
     1861 gatgtggatg acgggctgga taatacgacg cagtcctaca cgattcatgt cgacaaggaa
     1921 aaggcagcgg aatacggcat gacaacagcg caggtattcc agctggtcta tgcaaaattg
     1981 gcgtcccaga ccgcggtgac gacgatctct gtcggcgtgc aggattacga ggtgtatgta
     2041 aggagcgagg agcaggcgaa tgtgacgctc gacgatatta agaatctcac gttttcctac
     2101 gaggataaga tggcgcagct aagcggcaca ggtagcgatg aggagaaaga gacggacgaa
     2161 aaactgtcgg aggatatcaa ggatacaggg gatgccaagg atatcgaaga cacagaagat
     2221 accggggata ccggggatac ggtaaaggag attccactta ccgagattgc gacgtttgag
     2281 gctggcagca gcttaaacgc agtgtccagg gattcccaga cgcgctatat cagcgtcaca
     2341 gcaggtgtgg atgacacaca caacgtcacg cttgtcgcaa acgcactgat gaaagaactg
     2401 aataaaatag atctgccgga aggatactca attgagatga cgggtgagga tgagatgata
     2461 gcagatgcca tgcagcagtt gtacctgat
//
LOCUS       DBMDQJ010000372         2460 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15038_length_2460_cov_1.292497, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000372 DBMDQJ010000000
VERSION     DBMDQJ010000372.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15038_length_2460_cov_1.292497"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="ACZO9R_07995"
     CDS             complement(<1..213)
                     /locus_tag="ACZO9R_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913042.1"
                     /translation="MIDYAKNNNIDIENSHYIMTYSEDSSQYYWWYYIFFIPDDILVN
                     DTLILTHGRYSSSFTNSFIQARVSNYG"
     gene            complement(297..1130)
                     /locus_tag="ACZO9R_08000"
                     /pseudo
     CDS             complement(297..1130)
                     /locus_tag="ACZO9R_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741907.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
     gene            complement(1150..>2460)
                     /locus_tag="ACZO9R_08005"
     CDS             complement(1150..>2460)
                     /locus_tag="ACZO9R_08005"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR0913043.1"
                     /translation="YMITTNVSYGGNFIVTAEHPEGPWSDPCYLGEEAAGIDPSLFFD
                     EDGRCYYCGTRPNPDGVRYNGDWEIWVQELDLTTKKLVGESMAIWKGAVRGAIWPEGP
                     HLYKKDGYYYLLHAEGGTGPEHSISVARSRKLFQWFEGCPRNPIFTHRNLGADYPVVY
                     AGHGDLVEDQNGNWYVVMLATRRCKNHGSMGRETFLAKVVWQDGWPVIAAGTGRLEKT
                     LEVPLKEYRFPEETGTCDTLCFWETRPDQRLLGIGGRNEASYSLTEKRGVFRLYCGKQ
                     TIGEKGCSSYFGLRQKSYHFHAAVRFRFEPQSEDETAGMVLYQNHENHLRMEIGKKGA
                     DVCFRVSTYVHAREEVIREVLLKEEQELWEILIRGEEQSAGIWVRLGEKMICACDKVS
                     LLAYTTEEAGGFVGCTIGMYASSHGTDSKNYADYVSFTCSGAEE"
BASE COUNT          602 a          752 c          446 g          660 t
ORIGIN      
        1 accatagtta gaaacacgag cctgaataaa agaattagta aaagatgatg aatagcgacc
       61 atgtgtaaga attaatgtat cattaaccaa aatatcatca ggaataaaga aaatgtaata
      121 ccaccaataa tattgtgaac tatcctcaga atatgtcata atataatgag aattttcaat
      181 atcaatattg ttattttttg catagtcaat catttgtgaa aatcgtttcc cacgaataac
      241 gttactagac gaaccaccac cgctaacatc atcagcaaac gcagggacgg cagaaaatac
      301 aaaaatgcct tcccgcagtt taaatcacag ccatggttga cagccattgc cgcagattct
      361 tctaccgtct ttgtcacatg atggtgctca tgaaaatcaa gaattgccca gcagtcggac
      421 actacgtgtc cctcaaaacc gaattgttct cttaaaatat cctgcagaag tgtcttgctt
      481 ccgcacgccg gttccccgtt gacgcggtta taagcgccca tcacggcttc cacaccggca
      541 tccttgacac accgcttaaa tgcatacaaa tacgtatcgt acaaatcgtg cagcgacacc
      601 tttgcatcaa actcatggcg caatgcttcc ggaccgctgt gcacggcaaa atgcttggca
      661 cacgcagccg cctttaaatg atccggatct ttgccctgaa tccctctgat atacgcacag
      721 ccaagctttg ccgtcagaca gggatcctcc ccgtacgtct catgtcctct cccccagcgt
      781 ggatctctaa aaatgttaat attcggcgcc cagaaagtca gacctttata aatacctctg
      841 tctcctcttc ctgaatacgc ttcaaacttt gcccgtccct cggtggacac cacatctccg
      901 atcttctcaa tcagttcttc atcaaaagtc gctgccaggg caattgcctg tgggaacatt
      961 gtcgccacac cggatcttgc cactccgtga agcgcctcat tccaccagtt atacgccgga
     1021 atatgcagcc gctcaatcgc cggtgattca tagcgcatct ggctgatctt ctcctccaac
     1081 gtcatctgtt ccaccagtct ttttgcatat tctttcgtct ccagactcat ctctccctct
     1141 ccttttgctc tattcttctg ctccactgca cgtaaagctc acataatccg cataattttt
     1201 actgtcagtt ccatgcgaag acgcgtacat tccaatggtg catcccacaa agcctcccgc
     1261 ctcttccgtg gtatacgcaa gcaggctgac cttatcgcag gcacagatca ttttttctcc
     1321 cagccttacc cagatacccg cactctgctc ctctccgcgg atcagaatct cccataactc
     1381 ctgctcttct ttcagaagaa cttcacggat gacctcttcc ctggcatgaa catatgtgct
     1441 cacccgaaag cagacatctg ctcccttttt cccgatctcc atcctcagat gattctcatg
     1501 gttctggtat aataccattc ccgccgtctc atcctccgac tgtggttcaa agcggaatct
     1561 gaccgctgca tggaaatgat agcttttctg acgaagtccg aaataactgc tgcatccttt
     1621 ttcaccgatc gtctgttttc cacagtataa gcggaataca ccgcgttttt ctgtcagcga
     1681 ataggatgcc tcatttcttc ccccgatccc aagcagcctc tgatccggtc tcgtctccca
     1741 gaaacacagg gtatcgcacg ttcctgtttc ttccggaaac cggtactctt ttagcggcac
     1801 ttccagtgtc ttctccagac gtccggttcc ggcagcaatt accggccatc catcctgcca
     1861 tacaactttt gccagaaaag tctcgcgccc catacttcca tggttcttac agcgtctcgt
     1921 ggcgagcatc accacatacc agttcccgtt ttgatcctcc accagatccc catgaccagc
     1981 atagaccaca ggataatccg ccccaagatt ccgatgtgta aagatcggat tgcgcgggca
     2041 tccctcaaac cattgaaaca gctttcggct tctcgccaca ctgatgctgt gttccggtcc
     2101 ggttcctcct tctgcatgca gcagataata atagccgtct tttttgtaca gatgtggtcc
     2161 ctccggccag attgcccctc tcacagcccc tttccagatt gccatgctct ctccgaccag
     2221 cttcttcgtc gtcagatcca gttcctgcac ccagatctcc cagtccccat tgtaccttac
     2281 tccatcggga ttcggtctgg ttccgcaata atagcacctg ccatcctcat caaaaaacag
     2341 gctgggatca atcccggctg cttcttctcc cagataacag ggatctgacc acggtccctc
     2401 cgggtgctcc gccgtcacaa taaaattacc tccatagctg acatttgtgg taatcatata
//
LOCUS       DBMDQJ010000373         2456 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15061_length_2456_cov_1.792781, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000373 DBMDQJ010000000
VERSION     DBMDQJ010000373.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15061_length_2456_cov_1.792781"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(262..1497)
                     /locus_tag="ACZO9R_08010"
     CDS             complement(262..1497)
                     /locus_tag="ACZO9R_08010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0913044.1"
                     /translation="MKHTIIYFIGVYDTLDLFTGKLREAFETMGYASFVYDARLPQSK
                     DALLSVLAESSSPEREFSCVTFNNLGYNLDMEVPAPAQSVRENDACIQKGGTRNVWEY
                     YGIPYLNILMDHPFHYEKPLRLAPETSVVLCTDRNHVKYIRRYFKNIHQVDFLPHAGV
                     ELGSRHKPLAERGIDVLYAGALPIYTVAKMIPDLSSIPEVDGQLMAGDVLAELVHHPD
                     RTTEEVIEEYLKDRRSDIPDKRVQEIIVQMRFIDSYATSFFREQAVRILVENGIRVTA
                     YGTGWDQCEWSGSPYLDYRGKVLAPEILPSMNDAKIVLNTMTWFKAGAHDRIFNGMLA
                     KAVVVTDDSTYLRREFTDGRELVMFRRQELGTLPERVFDLFGHLERAQEIADCGYAAA
                     RDGHTWKSRAEYLKECFLT"
     gene            complement(1521..>2456)
                     /locus_tag="ACZO9R_08015"
     CDS             complement(1521..>2456)
                     /locus_tag="ACZO9R_08015"
                     /inference="COORDINATES: protein motif:HMM:NF019623.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HIR0913045.1"
                     /translation="TKYETAFGFTEEEVKVGLARVGLLDYREKVKEWYDGFTFGQRRD
                     MYNPWSITKFIDAEGIFDTYWANTSNNKLVSSLIRKSSKNMKMAMEQLLEGEMLHVEM
                     DEQLDFAQLEYRESAIFSLLYATGYLRVNQKNDQEYVLMLTNKEVEIMFRRIIREWFN
                     DMDTGYGDFRRALLHDNIEEMNYYMNMVTLSTFSYFDTGTGRGNIDETERFYHGFVLG
                     LLADLSDQFSIRLNRESGLGRYDIILRAREENGNSYIMEFKVFDRERDENMKACVARA
                     LQQIEEKQYETELVADGIARERIRNMDLHLTGKRF"
BASE COUNT          599 a          735 c          447 g          675 t
ORIGIN      
        1 tcaaattcag gacttacgtc atagcttccg agttcctgtt cgatattgtc caccgtatgt
       61 ccaatcagca aaaaagtctg ctcaatgtct ctgtaattat atgctttcca tcggtacatc
      121 aatatgtgca tagctgtaca ttcctctatc ctatatattt ccttctttgc agtactgcgc
      181 gcagagactt accgcctttg ttcgccttac cgcgcgcaga agcttacatt taccgctccc
      241 atcttcgccc atgtgcattc gtcaggtcag aaagcactcc ttcagatact ccgcacggct
      301 tttccacgta tgaccgtctc tcgccgcagc atatccacaa tctgcaatct cctgtgcccg
      361 ctccagatgt ccaaacagat caaacaccct ctccggcagt gttccaagct cctgccgccg
      421 gaacatcaca agttccctgc catcggtaaa ttcgcggcgc agataggtag aatcgtcggt
      481 taccaccacc gctttcgcca gcattccatt aaaaatccga tcgtgcgctc ctgccttaaa
      541 ccaggtcatc gtgttcaaca caattttggc atcgttcata gacggaagga tctccggcgc
      601 aagaactttt ccacggtaat caagatacgg gctgccgctc cactcgcact gatcccatcc
      661 cgtaccatac gccgtgaccc ggattccgtt ttccaccaga atacgcaccg cctgctccct
      721 gaaaaacgaa gtggcgtagg agtcgataaa acgcatctgc acaatgatct cctgcacgcg
      781 cttgtccggg atatcgcttc gccggtcttt aagatactct tcaatgactt cctccgtcgt
      841 ccggtccgga tggtgcacca attccgccag cacatccccc gccatcagct gtccgtccac
      901 ttccgggatg gaagacagat ccggtatcat ttttgccacc gtataaatcg gaagcgctcc
      961 ggcatacaga acgtcaatcc ctctctccgc aagcggcttg tgccggcttc ccagctccac
     1021 ccccgcatgc ggaagaaaat ccacctgatg gatattttta aaatatctgc ggatatattt
     1081 tacgtgattc cggtctgtgc agagcacaac cgacgtctcc ggcgccaggc gcagcggctt
     1141 ctcatagtga aacggatgat ccatcagaat gttgagatac gggatgccgt aatattccca
     1201 gacatttctg gtcccaccct tttgtataca cgcatcgttc tcccgtacgc tctgtgccgg
     1261 cgcaggcacc tccatatcca gattgtatcc cagattatta aatgtcacac atgaaaattc
     1321 acgctccggc gaactgctct ccgcaagcac agaaagcagt gcatccttgc tctgcggcag
     1381 ccgcgcgtca tacacaaagg acgcatagcc catcgtctca aaagcttccc gcaattttcc
     1441 ggtaaataaa tcaagtgtgt cgtatacacc gatgaaatag ataatggtat gcttcaagat
     1501 actctccttc tatgagccga tcagaacctt tttcccgtca aatgcaaatc catatttctg
     1561 atccgctccc tcgcaattcc gtctgccaca agctctgtct catattgttt ctcttctatc
     1621 tgctgcaacg cccttgcaac gcacgctttc atattctcgt ctctctcccg gtcaaaaacc
     1681 ttaaattcca taatgtaaga attgccgttt tcttcccgcg cgcggagaat aatgtcatat
     1741 cttcccagac cactctccct gtttaaccgt attgaaaact gatctgataa atctgccagc
     1801 aatccaagca caaagccatg gtaaaagcgc tctgtctcat caatattacc acgccctgtg
     1861 cctgtgtcaa agtaactgaa ggtggatagc gtgaccatat tcatatagta gttcatctct
     1921 tctatattat catgcagcaa cgctcttctg aaatctccat agccagtatc catatcgtta
     1981 aaccattccc gtataatgcg tcgaaacata atctccactt ctttgttcgt cagcatcagc
     2041 acatattcct gatcgttctt ttggtttacg cgtagatacc ccgtcgcata tagaaggctg
     2101 aaaatagccg attcccgata ctccaattgt gcaaagtcca gttgctcgtc catctccaca
     2161 tgtaacatct ctccttcaag aagctgttcc atcgccatct tcatattctt tgaagacttt
     2221 cggattaacg agctcaccag tttattatta cttgtattcg cccaataagt atcaaagatc
     2281 ccctctgcat cgataaactt tgtgatcgac cacggattat acatatccct gcgctgtcca
     2341 aatgtaaatc cgtcatacca ttcttttact ttttccctgt aatcgagcag acctactctg
     2401 gcaagtccca cctttacctc ttcttccgta aagccaaatg ccgtctcata tttcgt
//
LOCUS       DBMDQJ010000374         2452 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15092_length_2452_cov_1.576350, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000374 DBMDQJ010000000
VERSION     DBMDQJ010000374.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15092_length_2452_cov_1.576350"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(21..296)
                     /locus_tag="ACZO9R_08020"
     CDS             complement(21..296)
                     /locus_tag="ACZO9R_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2508 family protein"
                     /protein_id="HIR0913046.1"
                     /translation="MLRFFQPEKTNDNHYTLLINDLHQTAQDLQDAYRNLENVIDPDL
                     IDCYIYELNSVQMRYKFLLTSIRNYELRTQKNPEDFYTKNPLELSES"
     gene            489..767
                     /locus_tag="ACZO9R_08025"
     CDS             489..767
                     /locus_tag="ACZO9R_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HIR0913047.1"
                     /translation="MQEMSLTSFWIIAGSCVLVLGIMILKNRARFALNFLVRAVLGAC
                     AIACVNKWLAQQGIMLAVGINPVTLLTTGSLGFSGVALLYGILLLKIL"
     gene            875..1315
                     /locus_tag="ACZO9R_08030"
     CDS             875..1315
                     /locus_tag="ACZO9R_08030"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HIR0913048.1"
                     /translation="MTLTLLTVLVFAVAADFREMRISNRLIASGLMMGLTLRILGEGG
                     AGIVHFLVNISIPVILLFLLFQLRVIGAGDIKLFSVAGGFLSMRQLLYVILAAFVTAA
                     VIGLGKLLYQKRMAGIFGNQRTLIHFSAMILIGYFIVVWGCAIE"
     gene            1308..2345
                     /locus_tag="ACZO9R_08035"
     CDS             1308..2345
                     /locus_tag="ACZO9R_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR0913049.1"
                     /translation="MNKIAVAVCDTDREYRDRFVTYLVERKAEEYAVSAFSMPEKLLE
                     ALEEKKPDLAICGTGFEEMAERLAGYDIPVLVLQETMADAVAEPVTYGSVPESCRGIC
                     RYQSMEVILHEVQVMTLTGAGSRRMENVGTGMEVIGVYSPICHEMQMPFSMVLAQKLS
                     TEKKVLYVNLMEHSGMLELLGVPGECDLGEVILALRRQRLTAETLGRGIHETEWMHYV
                     EPFDNPEDLGEMTAGDLQELISFLEKQMSYEILLLDFGQGLRDFLSFLGLCTSIYCPM
                     KSGFYYDCRMEHFRRYLEREPAADIADRMQVIRLPFSAGKIRAGMDVYRQFLWSEFGD
                     YVRNHIGGQEE"
BASE COUNT          616 a          458 c          730 g          648 t
ORIGIN      
        1 gacaatctgg tggagacttg tcaggattcc gatagttcca gaggattttt ggtataaaaa
       61 tcctctggat ttttttgtgt gcgcagctca tagttgcgga tgcttgtaag aagaaatttg
      121 tagcgcatct gcacggaatt taattcataa atgtaacagt cgatcagatc cggatcgatg
      181 acgttttcca gattgcggta ggcgtcctgc agatcctgtg ccgtctggtg cagatcgttt
      241 atcagcagcg tataatgatt atcattcgtt ttttcaggtt gaaaaaaacg cagcatagtt
      301 cctccaggtc tatgggttct gccgaagtct gcggcagtgt tgttccttct atcttattca
      361 aagtataagc aaataaatcc cattctatac aaaaaacggt aaaaatctgt ccggccgccg
      421 gaacagacaa accggcataa acggacgcgg acattcatag atatagaaaa aagtgtggag
      481 gaaacgccat gcaggaaatg tcgctgacat cattctggat cattgcggga agctgtgtgc
      541 tggtccttgg aattatgata ttaaaaaatc gggcaaggtt tgcattgaat tttctggtgc
      601 gcgcggtctt gggtgcctgc gccatcgcat gtgtgaacaa atggctcgca cagcagggaa
      661 tcatgcttgc cgtcggcatc aatccggtga cccttttgac aaccggaagc cttggtttta
      721 gcggggttgc gcttctttat ggaattttgc tgctcaaaat tttgtgagaa tcttacaaat
      781 gtaaataaaa tgcgaaaaac ctatagacaa agcaaaaatg aattgctata atccgattta
      841 caaatgaacg aaggggtggt gagttgggga acttatgaca ctgacgctgc tgacggtact
      901 tgtatttgcg gtcgcagcgg acttccggga gatgcggatc agtaaccggc tgattgcttc
      961 cggacttatg atgggactta cactgcgcat tctgggggaa ggcggtgcgg gcattgttca
     1021 tttcctggtg aacatttcta ttccggttat cttattgttt cttttatttc aactgcgtgt
     1081 aattggcgca ggagatatca agttattttc cgtagctggt ggatttcttt caatgagaca
     1141 gttgttatat gtcatactgg cggcatttgt gacggcagca gtaattggac tggggaagct
     1201 gctgtatcag aagcgaatgg cgggaatatt cgggaatcag agaacactga ttcatttttc
     1261 agcgatgatt ttaattggat attttattgt agtctggggg tgtgccattg aataaaattg
     1321 cggtggcagt ctgtgataca gaccgggaat accgggacag atttgtcaca tacctggtgg
     1381 aacgcaaggc ggaagaatat gcagtatccg cattttcgat gccggagaag cttttggaag
     1441 cgttggagga aaagaagccg gatcttgcga tatgcgggac cggatttgaa gagatggcgg
     1501 agagactggc gggatacgat attccggtgc tggtcttgca ggagacgatg gcagatgcgg
     1561 ttgcggaacc ggtaacgtac ggttccgtgc cggaaagctg ccgtggaatc tgccgttatc
     1621 aatccatgga ggtcattctg catgaggtgc aggtgatgac tctgacggga gccggcagca
     1681 gaaggatgga gaatgtcgga acgggaatgg aggtaattgg tgtttactct ccgatctgtc
     1741 atgaaatgca gatgccgttt tcgatggttc tcgcacagaa gctgtcgacg gagaagaagg
     1801 tattatatgt gaatctgatg gaacattcag ggatgctgga gctgcttggc gttccgggag
     1861 aatgtgatct gggcgaggtg attctcgcat tgcgcaggca gcgccttacg gcggaaacgc
     1921 ttggcagagg gattcatgag accgagtgga tgcactatgt agagccgttt gataatccgg
     1981 aggatctggg ggagatgacc gcgggggatc tgcaggaact gatctccttt ctggagaagc
     2041 agatgtcata tgagatcctc ctgttggatt ttggtcaggg gttaagggat tttctatcct
     2101 ttctggggct ttgcaccagt atttattgtc cgatgaagag cggcttttac tacgactgcc
     2161 ggatggaaca tttccgcaga tacctggaaa gagagccggc ggcggacatt gcagaccgga
     2221 tgcaggtgat aaggctcccg ttttccgcag gaaagatacg tgcgggaatg gatgtgtacc
     2281 gtcagttttt gtggagtgaa tttggggact atgtccggaa ccatatcggt ggacaggaag
     2341 aataagaccg ggacaggggg atgcgcaatg cagattgcgg gagaagagat agaacggctg
     2401 cgcagacaga tcatggagca gatcgacctg acgcggacgg tggaggacga gg
//
LOCUS       DBMDQJ010000375         2448 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15125_length_2448_cov_1.227524, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000375 DBMDQJ010000000
VERSION     DBMDQJ010000375.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15125_length_2448_cov_1.227524"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            38..457
                     /locus_tag="ACZO9R_08040"
                     /pseudo
     CDS             38..457
                     /locus_tag="ACZO9R_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            569..1819
                     /gene="hisS"
                     /locus_tag="ACZO9R_08045"
     CDS             569..1819
                     /gene="hisS"
                     /locus_tag="ACZO9R_08045"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR0913050.1"
                     /translation="MALSKKPVNGMKDILPEEMQIRDYVQQVIKETYRSFGFTPIETP
                     CMENIANLSNKQGGENEKLIFKVMKRGEKLKIAEAKEEADLVDFGMRYDLTVPLVRFY
                     ANNANDLPSPFKALQMGSVWRADRPQRGRYRQFMQCDIDIIGEPSNLAEIELILATTT
                     TIGRLGFKNFEIRINERRILKAMAAYSGFPEEEFDTVFIILDKMDKIGLDGVAEELAK
                     EGYAKESIDKYLALFEGVSGAQNGIKYLADTLQGFLEPEVEQNLQEIIDSVETAKTAD
                     FKMVFDPTLVRGMSYYTGTIFEIAMPELGAACGGGGRYDKMVGKFTGNDVPACGFSIG
                     FERIILLLMESGFTVPMQRPKVAYLIEKGCTSEQVSKVIARAQEERVAGKQVLVVRMN
                     KNKKFQKEKLTAEGYRDIVEFYKD"
     gene            1994..>2448
                     /locus_tag="ACZO9R_08050"
     CDS             1994..>2448
                     /locus_tag="ACZO9R_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007156523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HIR0913051.1"
                     /translation="MGRIIGIDLGTTNSAAAVWENGESHLIPNAFGDYLTPSVVSIGK
                     DGTVYVGKIAKERLVTHPEDTVSVFKRFMGTEKIYSLAGKKYRPEELSALVLKRLKED
                     AEHYLGEEITEAVISVPAYFNDLARNATKNAGELAGFHVERIINEPSAAS"
BASE COUNT          703 a          455 c          722 g          568 t
ORIGIN      
        1 gtacatcctt gcgtactatg ttccttgaag gtcgcggctt gtagaagagg tcctgacgga
       61 tctggaacca cttgcaatgg agaaaaatat aaggcttatt ggaaaatgta aggatgtaac
      121 tatggtggga agcgatatcc ttatttacag actggtatat aatcttgttg agaatgctat
      181 aaaatacaat cgtccgggcg ggcaggttgt tgtgacggcg gagtggaaag aaaaacatgt
      241 ttttctttcc gtagaggata caggaagcgg aattccagag gaactcagaa aacgggtatt
      301 tgaaccattc tttcgtgtgg ataaatccag aagcagggaa ctggggggag taggccttgg
      361 acttgcgctg gtttatgaaa ttgtgagagt gcatgatggc aatattgtaa tcagggacaa
      421 tccgtctggg ggcacagttt ttgaagtgat tttttaacaa caggatcata tttgcgataa
      481 atgattgtat ggtccttgat gcttgacgtg caggaacaca aaacgtataa taatcggagg
      541 atataaggca gaaaatggag gaacgctcat ggcattaagc aagaagccgg tcaacggcat
      601 gaaagatatt ttaccggaag agatgcagat ccgggattac gtgcagcagg ttatcaagga
      661 gacctaccgc agttttggat tcacaccgat cgagacacct tgcatggaga acattgcaaa
      721 tttgagcaat aagcagggag gcgagaacga gaagctgatc ttcaaagtga tgaagcgtgg
      781 tgagaagtta aagatcgcgg aggcaaagga agaggcggat ctggtcgatt ttggaatgcg
      841 ttacgatctg acggtgccgt tagtccgttt ctatgcgaac aacgctaatg atctcccgtc
      901 cccgttcaag gcactgcaga tgggcagtgt gtggcgtgca gaccgcccgc agcgtggacg
      961 ttaccgccag tttatgcagt gtgatattga tattatcggt gagccgagca atctcgcgga
     1021 gatcgagctg atccttgcga cgacaacgac gatcggcaga ctcggattca agaactttga
     1081 gatccgcatc aatgagcgcc gcatcttaaa ggcgatggca gcgtacagcg gtttcccgga
     1141 ggaagaattt gataccgtat tcatcattct ggacaagatg gacaagatcg gtcttgacgg
     1201 cgtggcagaa gaactcgcaa aggaaggcta tgcgaaggag tcgattgaca agtatctggc
     1261 gctgtttgag ggcgtatccg gggcacagaa cggtatcaaa tatctggcag acaccttaca
     1321 gggctttttg gagccggagg tggagcagaa tctccaggag attatcgata gtgtggagac
     1381 ggcgaagaca gccgacttta agatggtatt tgatccgacg cttgtgcgcg gaatgagcta
     1441 ttatacagga acgatttttg agatcgcaat gccggagctg ggagcagcgt gcggcggcgg
     1501 cggacgttac gacaagatgg tcggcaagtt caccggaaat gacgttccgg cgtgcggatt
     1561 ctccatcggc tttgagcgca tcattcttct tctgatggag agcggtttca ccgtgccgat
     1621 gcagcgcccg aaggtggcat acctcattga aaaaggctgt acctcggagc aggtatcgaa
     1681 agtgatcgcc agggcacagg aggagcgtgt tgctgggaag caggtgcttg tcgtgcgcat
     1741 gaacaagaac aagaagttcc agaaagagaa gctgacagcc gagggttaca gggacattgt
     1801 cgagttttac aaagactgat ccggaaatga tctgacagag aacgccatag gatgatctat
     1861 ggcgtttttt acataatggg aaacttcgtt tctattacat aaaaaagctt cgcgtggatg
     1921 tgcacgtttc ttaaaggaga ttacgaacgg tataaagtaa aaaaaagaga aacagcttgc
     1981 agatggaggg catatgggaa gaattatcgg tattgatctt ggaacgacaa acagtgcggc
     2041 ggcagtgtgg gaaaatggag agagtcatct gattccgaat gcgtttggag actatctgac
     2101 gcctagcgta gttagtatag gcaaagatgg cacggtgtat gtgggaaaaa ttgcaaagga
     2161 gcgtctggtt acacacccgg aggatacagt aagcgttttt aaacgattta tgggaacgga
     2221 aaagatatat tcgctcgccg gaaaaaaata ccgtccggag gaactttccg cgcttgtgtt
     2281 aaaaagatta aaggaagacg cggagcatta tctgggggag gagattacgg aagcggtcat
     2341 cagtgtaccg gcatatttta acgaccttgc aagaaatgca acaaaaaatg cgggggaact
     2401 ggcaggattt catgtggagc gcatcatcaa tgaaccttcg gctgcatc
//
LOCUS       DBMDQJ010000376         2434 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15224_length_2434_cov_1.494213, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000376 DBMDQJ010000000
VERSION     DBMDQJ010000376.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15224_length_2434_cov_1.494213"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="ACZO9R_08055"
     CDS             complement(<1..1275)
                     /locus_tag="ACZO9R_08055"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR0913052.1"
                     /translation="MQQKDNDQFSFINEKIKEKPVNKKRLLMQAGFIALMAVVFGIIA
                     SLVFAYFQPKFESLFYPEEEPVVTIPQDSVAVTELTEETETSEETEASEEPQAPSEEP
                     PLQQPQELEIADVQNVQNKLYAVGREANRFVVTVTGVKSDTDWFNNSYESRGQASGII
                     IADSGQELLILTERKVISDAQEVYVTFINDVTVEASMKHYDGNTGIAVLSVPRSEVDE
                     DTMNAISVAKLGNSLTTMQGTMVIAVGSPLGTNYSILTGNITSTNNVISTIDNNYTVF
                     TTDIVGSSSGSGVIINVNGEVIGLVMQDYGNEEDQTTLTALSISELKQLIEKLSNNQD
                     IPYIGLGLTTVSAATAKEYDIPKGAYIKEVKMDSPALAAGLQSGDVITEMDGEAVYTV
                     DSYESKLLALKPGDEVEIVVQRQGAEKYEEITC"
     gene            1465..>2434
                     /locus_tag="ACZO9R_08060"
     CDS             1465..>2434
                     /locus_tag="ACZO9R_08060"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA];
                     GO_process: GO:0045910 - negative regulation of DNA
                     recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS2 family protein"
                     /protein_id="HIR0913053.1"
                     /translation="MNEKVLHTLEYNKILDQLTEYAFSADAKSRCQKLRPITDRAQIE
                     QLQQQTSDALSRLFKYGSLSFSGVTDIRDSLKRLEIGGALSAIELLRVCSLLESAKRA
                     KAFARSQDDNDQPDDSLTSLFAGIEPLTPLYDEIRRCILSEDEIADDASSTLHSIRRS
                     MRGMNDKIRAQMNSMINNTTTRSYLQDTVITMRDGRYCLPVKAEAKSLVPGMIHDQSS
                     TGSTLFIEPMAVVNLNNEYKELQLREQEEIEVILAGLSNLTASYATQLLADYELLTEL
                     DFIFARAAFAQTYNGVAPLFNDDGRIHIRKGRHPLLDPKKVVPIDVR"
BASE COUNT          567 a          721 c          545 g          601 t
ORIGIN      
        1 acaggtgatc tcctcgtact tctccgcgcc ctgccgctgt acaacgatct ccacctcgtc
       61 gcccggtttc aatgccagaa gcttgctctc ataactgtcc accgtgtaga cagcttctcc
      121 atccatctct gtaatgacat cgccgctctg cagtcctgcc gccagcgctg gtgaatccat
      181 cttgacttcc ttgatgtatg cacccttcgg aatgtcgtat tccttggcgg ttgccgcaga
      241 aaccgtcgtc agtcccagac cgatatacgg aatgtcctga ttgttggaca gcttctcgat
      301 caactgcttt aactcggaga tcgagagggc tgtgagcgtc gtctgatcct cctcattgcc
      361 gtaatcctgc attaccagac cgatcacctc gccgttcaca ttgatgatga cgccgctgcc
      421 gctgctgctg cccacaatat ccgtcgtaaa gacggtataa ttattgtcga tcgtggaaat
      481 cacattgttg gtagatgtaa tattccccgt aaggatcgaa tagttcgttc cgagcggact
      541 tcctaccgca ataaccatgg ttccctgcat tgtagtcagt gaattcccga gcttcgccac
      601 cgagattgca ttcatggtat cctcatccac ctcactgcgc ggcaccgaga gcactgcaat
      661 tcccgtattg ccgtcgtagt gcttcatgga cgcttccacc gtcacatcat tgatgaaagt
      721 aacatagacc tcctgtgcat ctgagatgac cttgcgctcc gttaagatca gaagttcctg
      781 accgctgtct gcaatgataa tgccggatgc ctgtcctctg ctctcgtaag aattgttgaa
      841 ccagtccgtg tcgctcttga cccccgtcac ggtcacgaca aaacggtttg cttctcttcc
      901 caccgcatac agcttgttct gcacgttctg tacatccgca atctcaagct cctggggctg
      961 ctgtagcggc ggttcctcgg aaggtgcctg tggttcctcg gatgcctccg tctcctcgga
     1021 tgtctctgtc tcctcagtca gctccgtcac cgcaaccgaa tcctgcggaa tggtgaccac
     1081 cggttcctcc tcggggtaaa acagtgattc aaacttaggc tgaaaatacg caaaaaccag
     1141 acttgctatg attccaaaaa cgactgccat cagggcgata aaacctgcct gcatcagcaa
     1201 tcgcttctta ttgaccggct tttctttgat tttttcgtta ataaaagaaa actgatcgtt
     1261 atccttttgc tgcatgattt cctcttttct gcatgtattt acacacgaat ttctgaaaac
     1321 cgtaccagta atcattataa aatgaatcac atacgcttgc aataggattc tacaattctt
     1381 ttcaggttcc gctttccaag actccctgaa tcatatataa taagaaggac ataaccatgt
     1441 gacaatcaaa tcaaaggaac tgctatgaac gaaaaagtat tacacacctt agaatataac
     1501 aagattctcg atcaactgac cgaatatgct ttcagcgctg acgccaagtc gcgctgtcag
     1561 aagctgcgcc cgatcaccga ccgcgcgcag atcgaacagc tgcagcagca gacatcggat
     1621 gccttaagcc ggctgttcaa atacggctcc ctgtcctttt ctggagttac ggacatccgc
     1681 gattcgctaa agcgcttaga gatcggcggc gccttaagcg ccatcgaact gctgcgcgtc
     1741 tgctccctct tagagtccgc gaaacgcgcg aaggcttttg ccagatcaca ggacgacaac
     1801 gatcagccgg acgattccct gaccagtctt tttgccggca tcgagcctct cacgccgctg
     1861 tatgacgaga tccggcgctg tattctctcc gaggacgaga ttgccgacga cgccagcagc
     1921 acgctgcact cgatccggcg ctccatgcgc ggaatgaacg ataagatccg cgcacagatg
     1981 aactccatga tcaacaacac cacgaccaga agctatctgc aggacaccgt cattaccatg
     2041 cgcgacgggc gctactgtct cccggtcaaa gcggaggcaa agtctctcgt tcccggcatg
     2101 atccatgacc agtcctccac cggctccaca cttttcatcg agccgatggc tgtcgttaat
     2161 ctgaacaacg aatacaaaga gctgcagctc cgtgagcagg aggagatcga ggttatcctt
     2221 gccggcttaa gcaatctgac cgcctcctac gccacacagc tgctcgccga ctatgaactt
     2281 ctgaccgagc tggactttat ctttgccaga gcggctttcg ctcagacata caacggggtt
     2341 gccccgctgt tcaatgatga cgggcggatt cacatccgca agggacgcca cccactactc
     2401 gatccgaaaa aggttgttcc gatcgacgta cggc
//
LOCUS       DBMDQJ010000377         2430 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15256_length_2430_cov_1.321597, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000377 DBMDQJ010000000
VERSION     DBMDQJ010000377.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15256_length_2430_cov_1.321597"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            2..964
                     /locus_tag="ACZO9R_08065"
     CDS             2..964
                     /locus_tag="ACZO9R_08065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0913054.1"
                     /translation="MASQPEVTVIIPNYNGKKLLENCIQTLERQTCTDFKLLVVDNGS
                     DDGSTEVISSLDLTMLALKENTGFCGAVNEGIRRADTPYVILLNNDTEVLPEFVEELL
                     AAIKKSDRIFSAGAMMIDYHDRGRIDNAGDYYTAFGWAVARGKGKPLADFNRPCRVFS
                     CCGGAVIYRTGLLREMGPFDERHFAYLEDVDMGYRAKIHGYINCYAPGARVYHVGSAT
                     TGTRYNEKKVFLAARNSMYLIYKNMPFLQLLINLPLILSGILIKSLFFLKKGFAGEYL
                     RGIGAGITGCRECKKVRFSWKNLGNYALIQLALWGNVIRILAGR"
     gene            1102..>2430
                     /locus_tag="ACZO9R_08070"
     CDS             1102..>2430
                     /locus_tag="ACZO9R_08070"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HIR0913055.1"
                     /translation="MIKDNQQYFNKLHVLIDALVIIVSYVFAWWLKFLSGILDHEVGV
                     LSFEFYMRALLLIVPVYILLYYAFNLYTSKRVQGRRLEFSNIVMANTVGLLLMFAALF
                     TLTSYNPQYRNFSREMLFYFYVTNIVVEEIVRLLIRRFLRSIRRRGYNLKHILLVGYS
                     RAAEQYIDRIQQNPQWGYNVRGVLDDNIARGTSYKGVKVIGSIGNLNYILPQNSLDEI
                     AITLGLEEYYKLEKIVAECEKSGVHTKFIPDYGNIIPTRPYTEDLLGLPVINIRYVPL
                     SNTFNAFVKRCMDVVGSIVCIILFSPVMLLSAILVKATSKGPLIFAQERVGLHNKPFQ
                     MYKFRTMYVQTEEEEQKGWTKKNDPRVTGVGKFLRKTSLDEFPQLFNVLKGDMSLVGP
                     RPERPQYVEKFREEIPRYMIKHQVRPGMTGWAQVNGYRGDTSIRKRIEHDL"
BASE COUNT          621 a          510 c          691 g          608 t
ORIGIN      
        1 catggcgagt cagccggaag ttacggtgat tattccgaat tacaacggaa agaagctttt
       61 ggaaaattgt atacagacct tagagagaca gacctgcacg gattttaagc ttctggtggt
      121 ggacaacgga tcggacgacg gcagtacaga ggtgatctcc agcctggatc ttacgatgct
      181 tgcgctaaag gagaatacgg gcttctgcgg agcggtgaat gagggcatcc ggcgcgcgga
      241 tacgccgtat gtgatcctgc tcaacaacga cacggaggtt ctgccggaat tcgtggagga
      301 gctgctggct gcgatcaaaa aatcggaccg catcttttct gcgggggcaa tgatgattga
      361 ttatcacgac agagggcgca tcgataatgc cggcgactat tacacggcat tcggatgggc
      421 ggtggcgcgc ggaaaaggaa aaccgctggc ggattttaac agaccgtgcc gtgtgttttc
      481 ctgttgcggc ggcgctgtca tataccgcac cgggctgctg cgtgaaatgg ggccgtttga
      541 cgagcgccat tttgcatatc tcgaggatgt cgatatgggg tatcgggcaa aaatccacgg
      601 atacatcaac tgctatgcgc ccggagcgcg ggtgtatcat gtggggagcg ctaccacggg
      661 gacgcgctat aatgagaaaa aggtgttcct cgcggcgcgc aattcgatgt atctgatcta
      721 caagaacatg ccatttttac agctgctgat caacctgccg ctgatccttt caggtattct
      781 catcaagagt ctgtttttcc tgaaaaaggg gtttgcggga gaatatttaa ggggaatcgg
      841 cgccgggatt accgggtgcc gggaatgtaa aaaagtgcgg ttctcatgga aaaatctggg
      901 gaattacgcg ctcatacagc tggctttgtg gggcaatgtg atccggattc tggcaggaag
      961 ataaatttga tcgtcaaaca aatctttaaa aaatattaag aaaatgttac agaatgtttg
     1021 cgaaaaaggc gttgtgcttt tatggaaatt attgtatcat tgatgtcata tggtagttag
     1081 aattcgcaaa aggatagcaa tatgataaaa gacaatcagc agtactttaa taagctgcat
     1141 gtactgatcg atgccttggt tatcatcgta tcgtatgtgt ttgcatggtg gctgaagttc
     1201 ctgagcggta ttctggatca tgaagtcggc gtactctcat ttgagttcta tatgagggcg
     1261 ctgctgctca ttgttccggt gtatattttg ttgtattatg cgttcaatct gtacacgtcc
     1321 aagcgcgtgc aggggagacg gctggagttt tccaacattg tgatggcgaa tacggttgga
     1381 ctgctgctga tgtttgcggc tctctttacc ctgacgtctt acaatccgca gtatcgcaac
     1441 ttctcacggg agatgctttt ctatttctac gtgaccaata ttgtggtgga agagattgtc
     1501 cgccttttga ttcgcagatt tctgcgaagt attcgcagaa ggggatacaa tttaaagcac
     1561 attctgctcg tcggctattc aagagcggca gagcagtata tcgacagaat tcagcagaat
     1621 ccgcagtggg gatacaatgt gcgaggggta ttggatgaca atattgccag aggcacctcc
     1681 tataagggag tgaaggtcat cggcagtatt ggtaatctca actacatcct gccgcagaac
     1741 agtctcgatg agattgcaat caccctcgga ctggaagaat attataagct ggaaaaaata
     1801 gttgcggagt gtgagaagtc cggcgtgcac accaagttca ttccggatta cggcaacatc
     1861 atacctacga gaccttacac ggaggatctt ctgggtctcc ctgtgatcaa tatccgctat
     1921 gtgccgctgt ccaacacgtt caacgcattt gtgaagcgct gtatggatgt ggtgggatcg
     1981 attgtgtgta tcatcctctt ttcaccggtg atgctgctgt cggcaatcct tgtcaaggcg
     2041 acatcgaagg gaccgctgat ctttgcacag gagagagtgg ggctgcacaa taagccgttt
     2101 cagatgtaca agttccgcac catgtatgtg cagacggagg aggaagaaca gaaaggctgg
     2161 acgaagaaga acgatccgcg cgtcacagga gtcggaaagt tcttgcgcaa gacaagcctg
     2221 gacgaattcc cacagttatt taatgtgctg aagggcgata tgagtctggt gggaccaaga
     2281 cccgagcgtc cgcagtatgt agagaaattc cgggaagaga tcccgagata tatgatcaag
     2341 catcaggtgc gtccgggaat gaccggctgg gcccaggtca acggataccg tggagacacg
     2401 tcgatccgca agcgcattga gcatgatctg
//
LOCUS       DBMDQJ010000378         2415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15366_length_2415_cov_1.705272, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000378 DBMDQJ010000000
VERSION     DBMDQJ010000378.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15366_length_2415_cov_1.705272"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            55..795
                     /gene="rlmB"
                     /locus_tag="ACZO9R_08075"
     CDS             55..795
                     /gene="rlmB"
                     /locus_tag="ACZO9R_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HIR0913056.1"
                     /translation="MGYEELKIEGRNAVLEAFRSGKTIDKLFVLDGCQDGPVRTTVRE
                     AKKHDTIIHFVEKERLDQLSETKKHQGVIAVAAAYEYAEVDDILEIAREKGEPPFLFL
                     LDNIEDPHNLGAIIRTANLCGAHGVIIPKRRAVGLTATVAKTSAGALNYTPVAKVTNL
                     TATIKELKEQGIWFVCADMGGTQMYDLDLKGPIGLVIGSEGEGVSKLVRENCDYVASI
                     PMKGDIDSLNASVAAGVLAYEIVRQRMM"
     gene            829..1497
                     /gene="sigH"
                     /locus_tag="ACZO9R_08080"
     CDS             829..1497
                     /gene="sigH"
                     /locus_tag="ACZO9R_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HIR0913057.1"
                     /translation="MLRKIQPCAERLGGIAGQRGKTEEDVDEYQKLADEELIQKLREG
                     DERIMDYILEKYKPLVLRKANAMFLIGGDTDDLIQEGMIGLFKAIRDYRSDRETSFFH
                     FAELCINRQLYSAVEASNRKKHVPLNTYVSFYSQTTEEGKSLAETLLTDQMDDPEQLV
                     IEQENFTAFWEQLREQLSALERQVLDAYLEGKNYRQIAEELGKSPKTIDNALSRIKGK
                     IRQR"
     regulatory      1851..1959
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2034..>2415
                     /locus_tag="ACZO9R_08085"
     CDS             2034..>2415
                     /locus_tag="ACZO9R_08085"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015526221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR0913058.1"
                     /translation="MEQYATQMEAARKGIVTEELKKVAAKERMTTEELMPLVAEGKVV
                     ICANRHHKCIDPEGVGSMLRTKINVNLGISRDCKDYDVEMEKVMAAVDMGAHAIMDLS
                     SHGNTIPFRRKLTAECPAMIGTVPV"
BASE COUNT          699 a          488 c          726 g          502 t
ORIGIN      
        1 gatgctgcag gcagcagaaa gcgcctgccg gaagcagaaa gaaaaggaga atacatggga
       61 tacgaggaat taaaaataga gggacgcaat gcggtgcttg aggcgtttcg ttccggcaag
      121 acaatcgaca agctttttgt gctggacgga tgtcaggacg gaccggtgcg cacgaccgta
      181 agagaagcaa aaaagcacga taccattatc cattttgtgg aaaaagagcg tctggatcag
      241 ctctcggaga cgaagaagca ccagggcgtg atcgcggtgg cggcagcata tgaatatgcg
      301 gaagtggatg acattctgga gatcgcaaga gaaaagggag agccgccgtt cctttttctg
      361 ctggataata ttgaggaccc gcacaatctc ggggcgatca tccgtaccgc gaacctctgc
      421 ggagcacacg gggtcattat tccaaagcgc agggcggtgg gactgaccgc gactgtggca
      481 aagacgtccg ccggcgcgct gaactatacg ccggttgcca aggtgacgaa tctcacggcg
      541 acgatcaagg agttaaagga gcagggcatc tggtttgtct gcgcggatat gggcggcacg
      601 cagatgtatg acctggatct gaagggaccg atcggtcttg taatcggaag cgagggtgag
      661 ggtgtcagca agctggtgcg cgaaaactgt gattatgtgg cttccattcc gatgaagggt
      721 gacattgatt ctctgaatgc ttccgtagca gccggtgtgc ttgcctatga gatcgtcaga
      781 cagcggatga tgtaagatga cagagcaggc ataggcggac agatttttat gctgcggaaa
      841 atacagccgt gcgcggaacg tttgggcggc atagcagggc agagagggaa gacggaggaa
      901 gacgtggacg aatatcagaa gctggcggat gaagaactca tccagaaact gcgcgaaggc
      961 gacgagcgga tcatggatta tatcctggaa aagtacaagc cgttggtgct tcgcaaggca
     1021 aatgcgatgt ttttgattgg cggagatacg gatgacctga ttcaggaggg gatgatcgga
     1081 ctgtttaagg caatccggga ttaccggtcc gacagggaga catccttttt ccattttgcg
     1141 gaattgtgca tcaacagaca gctctacagt gcggtggagg catccaaccg gaaaaaacat
     1201 gtacctctga atacttacgt gtccttttat tcccagacca cggaggaagg gaaatctctg
     1261 gcggagacgc ttctcaccga ccagatggac gatccggaac agctggtgat tgagcaggag
     1321 aattttaccg ctttctggga gcagctgcgg gaacagttaa gcgcgctcga gcgccaggtt
     1381 ttagacgcat atctggaagg aaaaaattac cgccagatag cggaggaact cggaaaaagt
     1441 cctaagacaa tcgacaatgc tctctcgcgt atcaagggaa agatccggca gagatagaga
     1501 gaaggcaaaa aggacatttt gcaaaaaaga gcaggaaatg aacaaccggt tatgcaaaat
     1561 tttgcggaaa caaaagaact agtcctaagg tactgtataa aatgtataga aatggaatgg
     1621 aagttttgtg aagtttgtgc acacttcttt acgcagtcat ggtactataa taaccgtaaa
     1681 aaccccccaa tacattatat atagtttttg ctatacccca tagtaaaaat accttctcct
     1741 aaaagggcag ccagtgatgg ctgttctttt tttgttatgg aaaagagcag ttctgaggaa
     1801 agggagattg acagaagcat agaattgaga taaggtgtta gaggaaaaag aatatatcgc
     1861 agagggactg tgcgcatgcg caaacggttg agaaggttaa aacctgactc ttggaacctg
     1921 tcagttaata ctgtcgtagg gagcgaatga tggatcagat agtttgtgga atccggcgtc
     1981 actgcgacgc cggatttttt cgcacatcct ctgcagaaat ggagcagaaa aatatggaac
     2041 agtatgcaac acagatggag gcagcccgca agggcattgt gacggaagag ttaaagaaag
     2101 ttgcggcgaa ggagcgcatg accacagagg agctgatgcc gctcgttgcg gagggaaagg
     2161 tggtcatctg cgcgaacagg catcacaaat gcattgaccc ggagggtgtc ggctcgatgc
     2221 tgcggacgaa gatcaatgta aatctcggaa tctccagaga ctgtaaggat tatgacgtgg
     2281 agatggaaaa agtgatggcg gcagttgaca tgggagcgca tgcgatcatg gatctctcct
     2341 cgcacgggaa caccattccg ttccggagaa aactgacggc ggaatgtccg gctatgatcg
     2401 gaaccgtgcc ggtat
//
LOCUS       DBMDQJ010000379         2415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15368_length_2415_cov_1.418755, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000379 DBMDQJ010000000
VERSION     DBMDQJ010000379.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15368_length_2415_cov_1.418755"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="ACZO9R_08090"
     CDS             <1..964
                     /locus_tag="ACZO9R_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002583666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0913059.1"
                     /translation="EGLAKQFDGNYLFSGIDFEIKRGERVALIGNNGTGKTTILKIIN
                     ELLLADGGTIVLGSNVHIGYYDQEHQLLHMEKTIFEEIADDYPQLNHTKIRNVLAAFL
                     FTNDDVFKRIADLSGGERGRVSLAKLMLSDANFLILDEPTNHLDITSKEILESALNQY
                     TGTVFFVSHDRYFINQTATRILDLTGGTIVNYIGNYDYYLEKHDELTRIYVEAEPKAQ
                     AAQMTEQTVAQDGSGTDKKADWQAQKAEQARIRKLENTLKKAEERIAELEDLIAGIDE
                     ECADPANATNSAKLGELTGRQNEYRSELEKCYEEWEQVSMELES"
     gene            1062..1724
                     /locus_tag="ACZO9R_08095"
     CDS             1062..1724
                     /locus_tag="ACZO9R_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR0913060.1"
                     /translation="MQEKEISQAVIRRMPRYYRYLGELLDAGVERISSNDLSLRMNVT
                     ASQIRQDLNNFGGFGQQGYGYNVKYLYEEIGKILGLNQQHNIIVIGAGNLGQALANYV
                     KFEKLGFMIIGLFDVNPALDGVTVRGIKIRMLDELEDFCRENKVDIAALTMPKEKADA
                     IANRLVGLGIHAIWNFAHVDLELINKDVVVENVHLSDSLMQLSYNIVKNQRAKENSRQ
                     TD"
     gene            1833..>2415
                     /locus_tag="ACZO9R_08100"
     CDS             1833..>2415
                     /locus_tag="ACZO9R_08100"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HIR0913061.1"
                     /translation="MRYIVNSREMNLYDSRTIEEFHMPQAVLMERAAFSFVETLRREK
                     RNTSRTLVVCGSGNNGGDGYAIARILMQAGNTVDVLEAPHTGKASVGNQLQKKIFLAY
                     GGKILTEFPEGKSYTLVIDAVFGVGLSRSVEGEYASLIDRMNAMEGAKAAVDIASGIS
                     ADNGNVLGTAFRADLTVTFAFEKVGCLLWPGNQY"
BASE COUNT          650 a          519 c          713 g          533 t
ORIGIN      
        1 ggaaggactg gcaaagcagt ttgacggcaa ttacctgttt tccggaatcg attttgaaat
       61 caaacgtgga gagcgtgtgg cgctgatcgg caataacgga accggcaaga cgacgattct
      121 taagatcatt aacgaactgc ttctggcgga cggcggaacc attgtgctcg ggtcgaatgt
      181 gcacatcggg tactacgatc aggaacatca gctgctccac atggagaaaa cgatctttga
      241 ggagatcgcg gatgactacc cgcagctaaa ccacacgaag atccgcaatg tcttggcagc
      301 gtttttattt acgaacgatg atgtattcaa gcggattgca gacttaagcg gcggggagcg
      361 cgggcgcgtc tcccttgcca agctgatgct ttccgatgcg aattttctga ttctggatga
      421 gccgaccaac catctggaca tcacatccaa agagattttg gagtcggcgt taaaccagta
      481 caccggaacc gtctttttcg tgtcgcacga ccgttatttt atcaatcaga ccgcaacccg
      541 gattctggat ttgacgggag ggacgattgt caactatatc gggaattacg attactatct
      601 ggaaaaacat gacgagctga cccggatcta tgtggaggcg gagccgaagg cgcaggcggc
      661 gcagatgacg gaacagaccg tggcgcagga cggaagtgga acggacaaga aagcggactg
      721 gcaggcgcag aaagccgagc aggcgcgcat ccgcaagctt gagaatacgc taaagaaggc
      781 ggaagagcgc attgccgagc tggaggatct gatcgccggc attgatgagg agtgcgcaga
      841 tcctgccaat gcaaccaatt ccgcgaagct cggcgagctg accggcagac agaatgaata
      901 ccggagcgag ctggaaaaat gctacgagga atgggagcag gtatcgatgg agcttgagtc
      961 gtgagggatg ccgtctggtt gttaaatttt tgacacattg acagatatct ggtttatcac
     1021 tataataaaa gagatgtttt gatagaaagg tgggcattac catgcaggaa aaagaaatat
     1081 cacaggctgt cattcgtcga atgccgcggt attacagata tctgggagaa ttgctggacg
     1141 ccggggtaga acgtatttct tcaaatgatc tgagcctcag gatgaatgtc accgcatccc
     1201 agattcgtca ggatctcaat aattttgggg gatttggcca gcagggatat ggatataacg
     1261 taaaatacct atatgaggaa atcggcaaga ttctaggact gaaccagcag cacaatatta
     1321 tcgtgatcgg tgccggaaat ctcgggcagg cactcgccaa ttacgtgaag tttgaaaagc
     1381 ttggatttat gatcatcgga ctgttcgatg tcaatccggc gttggacggt gtgactgtgc
     1441 gcgggatcaa gatccgtatg ctggatgagc tggaagattt ctgtagagag aacaaagtag
     1501 acattgcagc gcttaccatg ccgaaggaaa aagcggacgc cattgccaac cgtctggtcg
     1561 gtcttggcat tcatgcgatc tggaattttg cacatgtgga tctggaattg atcaataagg
     1621 acgttgttgt ggaaaatgtc catctctcgg acagcctgat gcagttatcc tacaatattg
     1681 tgaagaatca gcgcgccaaa gagaacagcc gtcagacaga ttaaaagagg tcctgcatag
     1741 caggatctct tttttactct atagggaact ttgttccagt gttttaccga aataaaatgc
     1801 cgggtgtgat gccggcaggc gggagggaaa agatgcgata tatcgtaaac agccgggaga
     1861 tgaatcttta tgacagccgg acaatcgagg aatttcatat gccgcaggca gtgttgatgg
     1921 agcgggcggc gttttccttt gtggagacac tgcgccggga aaaacggaat acgtcaagaa
     1981 cactcgtcgt ctgcggcagc ggcaacaacg gcggagacgg ctatgcgatc gctagaatac
     2041 tcatgcaggc gggaaacacc gtcgatgtat tggaagcgcc gcacacagga aaagcgtccg
     2101 tcggcaatca gctccagaag aagatctttc ttgcgtacgg cggtaagatc ctcacggaat
     2161 ttccggaggg gaagtcctac acgctcgtga tcgacgcggt attcggcgtg ggattatcgc
     2221 gcagtgtgga gggagaatac gcatcgttaa tcgaccggat gaatgccatg gaaggggcga
     2281 aggcggcggt tgacattgct tccggcattt cggcggacaa tggaaatgtg ctgggaaccg
     2341 ctttccgggc agatctgacg gtcacattcg cgtttgaaaa ggtgggatgc ctgctgtggc
     2401 cgggcaatca gtacg
//
LOCUS       DBMDQJ010000380         2415 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15369_length_2415_cov_1.184097, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000380 DBMDQJ010000000
VERSION     DBMDQJ010000380.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15369_length_2415_cov_1.184097"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            64..927
                     /gene="kduI"
                     /locus_tag="ACZO9R_08105"
     CDS             64..927
                     /gene="kduI"
                     /locus_tag="ACZO9R_08105"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HIR0913062.1"
                     /translation="MELRTAASPRDVKHYTTERLREEFLIDDLFLPDVIKLVYSHIDR
                     IITGSAVPVKETLKLTAGDELRAQYFCERRELGVINIGGAGTITIDGKAYHVAHKEGM
                     YIGMGSRDITFASDDAAAPAKFYLNSAPAHRTCPTVLIKPEGTPEDGVVIVKDCNKVE
                     LGSLETANHRTICKYILPGQVESCQLEMGMTKLEPGSVWNTMPCHTHDRRMEVYLYFD
                     MPEDALVMHYMGEPTETRHIVMRNEQAVISPSWSIHSGCGTQAYTFIWGMVGENQDFD
                     DMDDVAMTDLL"
     gene            947..1585
                     /locus_tag="ACZO9R_08110"
     CDS             947..1585
                     /locus_tag="ACZO9R_08110"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR0913063.1"
                     /translation="MKIALINENSQAVKNEMICNTLKSVVEPMGHTVFNYGMYSAEDE
                     HPLTYVQNGILAAVLLNSGAADFVITGCGTGEGAMLACNSFPQVLCGHVESPLDAYLF
                     SQVNDGNCIALPFAENFGWGGELNLTYIFEKLFCTPGGGGYPKERVVPEQRNKKILDE
                     VKKVTHRDLVSILKELDRDLVVGALSGEHFAEYFYANCKDDAIAACVKELLA"
     gene            complement(2177..2320)
                     /locus_tag="ACZO9R_08115"
     CDS             complement(2177..2320)
                     /locus_tag="ACZO9R_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913064.1"
                     /translation="MKRMMGFALFCFSMGMLVMLVLHSRFFGFLLLVLCMIAGYYLFC
                     CDD"
BASE COUNT          659 a          599 c          561 g          596 t
ORIGIN      
        1 cccattgaaa tgcccggttt tccgggcatt tcatgcaaca atacatagaa aggatatttt
       61 atcatggaac ttagaacagc tgcatctcca agagatgtca aacactacac caccgaacgt
      121 ttaagagagg aatttctgat tgacgacctg tttttgccgg atgtcatcaa gcttgtatac
      181 agccatattg accgtatcat cacaggttct gccgttccgg taaaagagac cttaaagctg
      241 accgccggcg acgaactccg cgcacagtat ttctgtgagc gccgtgagct tggcgtcatc
      301 aatatcggcg gcgcaggcac catcaccatc gatggaaagg cttaccacgt tgcacacaag
      361 gaaggcatgt atatcggcat gggctcccgc gacattactt ttgcaagcga tgacgccgct
      421 gcaccggcaa aattctattt aaacagtgct cctgcacacc gcacatgtcc aaccgttctc
      481 atcaagccgg aaggcacacc ggaagatggt gttgtcatcg tcaaggactg caacaaagta
      541 gaactcggtt ccctggagac agcaaaccac agaacgatct gcaaatatat tcttcctggt
      601 caggtagaga gctgccagct ggaaatgggc atgacaaaac ttgagccggg aagcgtatgg
      661 aacacgatgc cgtgccatac acacgacaga cgcatggagg tctatctgta tttcgatatg
      721 ccagaagatg cccttgtcat gcactacatg ggtgagccga cagagacacg ccacattgta
      781 atgcgcaacg agcaggcagt catctccccg agctggtcga tccactctgg atgcggcacc
      841 caggcttaca cctttatctg gggcatggtc ggtgagaatc aggattttga cgacatggac
      901 gatgtagcca tgacagattt gttataaaac ggaggtctaa accattatga aaatcgcttt
      961 aatcaacgaa aacagccagg cagtcaagaa tgaaatgatc tgcaacacat taaagtccgt
     1021 tgtagagccg atgggtcaca ccgtattcaa ttacggtatg tactctgccg aggatgagca
     1081 tccgctcacc tacgtccaga acggtattct tgccgctgta ttattaaatt ccggtgcagc
     1141 cgatttcgtc atcacaggat gcggtaccgg tgagggtgct atgcttgcct gcaactcctt
     1201 cccgcaggta ctctgcggac atgtagaatc tcctctggat gcttatctct tctcacaggt
     1261 caacgacggc aactgcatcg ctctcccgtt cgcagagaac ttcggctggg gcggtgagtt
     1321 aaacctcact tacatttttg agaaattatt ctgcacaccg ggcggcggtg gctatccgaa
     1381 agagcgtgtc gtgccggagc agcgcaacaa gaaaatcctc gatgaggtga aaaaagtcac
     1441 acaccgcgat ctcgtctcca tcttaaaaga gcttgaccgt gatctcgtcg tcggtgcatt
     1501 gagcggcgaa cattttgccg aatatttcta tgccaactgc aaggacgacg ccattgctgc
     1561 atgcgtaaag gaattgctcg cataagccgt tccaactaag taattgccgc aataagcatg
     1621 gatctctttg gagtgataag taatattata tgaaacacac ttggagattt catgtggaaa
     1681 tggagatgga aattcggttc aaaaaatgtg gaatttctaa ccaaccgaat gtttgcatct
     1741 tcgatgatcc gtgtctttcc aagtttggag cactccacgg actgtgtccg caagccagca
     1801 gacaatttta ggacttttgt gtgtgtccct tgcggcacca gacaacagat ggtataaatg
     1861 ccatctgttg tcgaataaaa cataaccgcg cgaagccgat gtttgagtcg gcatctgcca
     1921 tattttcaat agcagatgcc ggcgagtttc ggttcgcgcg gttttatatt gtctatgaag
     1981 aagtgccatt agggacacca gaaagtccgc tcattgtctg cggcttgtcg gacacactcc
     2041 gtgagtgtcc aaacgtggaa gagacacaga attcgatgca aacattcagc tggaagaaat
     2101 gctcacattt ttctccaacg aatataaagt ttgcataaat attttttccg tcttccctct
     2161 cctctgccgt gtgctctcag tcatcacagc agaacagata gtaaccggcg atcatgcata
     2221 ataccagaag aaggaaccca aagaaacggc tgtggagcac aagcatcacc agcatcccca
     2281 tggaaaagca aaacagtgca aatcccatca tccgcttcat aagatccccc aaacaatctg
     2341 tctcttcatc atatgcatct gccggaagtt tctctactgt tttttagttt tcttctttgt
     2401 tcacggaatc cttta
//
LOCUS       DBMDQJ010000381         2412 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15390_length_2412_cov_1.495457, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000381 DBMDQJ010000000
VERSION     DBMDQJ010000381.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15390_length_2412_cov_1.495457"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..920)
                     /locus_tag="ACZO9R_08120"
     CDS             complement(<1..920)
                     /locus_tag="ACZO9R_08120"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HIR0913065.1"
                     /translation="MSKEIALQQIRIKDPFWSGMQEKITDTVIPFQERVLNDKEEGVE
                     KSHALDNFRIAAGLMEGEFYGMVFQDSDVAKWLEGVAYSLVIKPDAALEQRADDIIDI
                     IAKAQQPDGYLNTFFTIKEPEHRWQNLLECHELYCAGHMMEAAVAYYEATGKDKLLGV
                     MERMAQHIMNRFGEDKIPGIPGHQEVEIGLMRMYHATGKEAYKDMARYFLEERGKNPN
                     FFKEETERRGWTHFGNMIPDDTKYNQSHATIYEQDEAVGHSVRAVYMYTAMADLAAED
                     HDEKLFAACRRLWENMTQKKMFITGGIGSTV"
     gene            complement(934..1587)
                     /locus_tag="ACZO9R_08125"
     CDS             complement(934..1587)
                     /locus_tag="ACZO9R_08125"
                     /inference="COORDINATES: protein motif:HMM:NF016726.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HIR0913066.1"
                     /translation="MNQFFDYNNNVFRVLGGLADCLILGALWIVCSIPVVTMGAATAA
                     VYHAVNKSIVHGQGYAFREYCTALKTDLKQTTGAWLLWGILAAFLAADLVVTRQFLAQ
                     GSALGALYYFFIVLSAMALAWEFYLTAYMARFEGTIRESMKKTLVMVVANLGWSALLV
                     AVFVLFVLMCNDTQCLIVLFPGVFALVKNYVLEKVFRKYRTPEDLARELEMTREYRN"
     gene            complement(1618..>2412)
                     /locus_tag="ACZO9R_08130"
     CDS             complement(1618..>2412)
                     /locus_tag="ACZO9R_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046556 - alpha-L-arabinofuranosidase
                     activity [Evidence IEA];
                     GO_process: GO:0046373 - L-arabinose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-arabinofuranosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR0913067.1"
                     /translation="LSLHYYTLPEVEDDWNKKGSATDFTEEIFYQTLKRGYFMDELIN
                     RHGAIMDEYDPDKKIGLIVDEWGIWTDVEPGTNPGFLYQQNTMRDALVAGMTLNIFNK
                     HSDRVKMACIAQLINVLQSVMLTDGEKMIKTPTYHVFHMYRHHQGATLLRSDLIGAGT
                     VGAGKNELPKVIESVSENADGVITVTLTNNSLEAAENVTIQLTDDGAAYGVCEASVVA
                     GTMNAHNTFEAPEVVTEQAFADYEKTADGIRVQIPACSVVSIRLKK"
BASE COUNT          519 a          744 c          516 g          633 t
ORIGIN      
        1 acggtcgagc cgatgcctcc ggtgatgaac atctttttct gcgtcatatt ctcccagagc
       61 cgtctgcacg ctgcaaacaa cttctcatcg tgatcctccg ccgcaagatc tgccatcgcc
      121 gtgtacatat atacggcgcg cacactgtgt cccacagcct catcctgctc gtagattgtc
      181 gcatggctct ggttgtactt tgtgtcgtcc gggatcatat tgccaaaatg cgtccagccc
      241 ctgcgctcgg tctcctcctt aaagaagttc ggattctttc cacgctcctc gaggaaatag
      301 cgcgccatgt ccttgtaggc ttcctttccg gtcgcatggt acatgcgcat cagaccgatc
      361 tccacctcct ggtgtcccgg aatgcccgga atcttatcct ctccgaaccg gttcatgata
      421 tgctgcgcca tccgctccat cacgccgagc agcttgtctt ttccggtcgc ctcgtaatac
      481 gccactgccg cctccatcat atggcctgcg cagtagagtt catggcactc taacagattc
      541 tgccagcggt gctccggctc cttgatcgtg aaaaatgtat tcagataacc gtccggctgc
      601 tgtgcctttg cgataatgtc aatgatgtcg tccgcgcgct gttccaacgc cgcatccggc
      661 ttgatcacga gcgagtacgc cacaccttcc agccattttg ccacatcgct gtcctggaat
      721 accatcccgt agaactcacc ttccatcagt cctgccgcaa tgcggaaatt atccagcgca
      781 tggctcttct ccacgccttc ttctttatcg tttaataccc tctcttgaaa cggtatcaca
      841 gtatctgtga tcttttcctg cataccgctc cagaacgggt ctttgatccg aatttgctgt
      901 aatgcaatct ctttgctcat ctcttttccc ccatcagttc cgatattcac gggtcatttc
      961 caattctctg gcgagatcct ccggcgtcct gtacttgcgg aataccttct cgagcacata
     1021 gtttttcacc agtgcaaaca ctcccggaaa cagtacaatc aggcactgtg tatcgttgca
     1081 catcagaaca aacagcacaa acacagctac taaaagcgcc gaccatccca gatttgccac
     1141 caccatcaca agcgtcttct tcatggactc ccgtatggtt ccctcaaacc tcgccatata
     1201 cgccgtcagg taaaactccc acgccagcgc catggcagaa agtacaataa aaaaataata
     1261 cagcgcacca agcgccgagc cctgtgccag aaactgtctg gtcaccacaa ggtccgccgc
     1321 aagaaacgct gccagtattc cccacagcag ccatgctcct gtggtctgtt tcaaatccgt
     1381 tttcagagcc gtgcaatatt cccggaatgc atagccctgt ccatgtacaa tgcttttatt
     1441 gaccgcatgg tacaccgccg ctgtggcggc tcccatggta acgaccggaa tgctgcatac
     1501 aatccacagc gcgccgagaa tcaggcagtc cgccagtcct cccagtacgc ggaaaacgtt
     1561 gttgttgtaa tcaaaaaact gattcatatg ccctccattc cggtctaagc cattcggtta
     1621 ttttttcaga cggatgctta ccacgctgca tgccggaatc tgtacccgta tgccgtccgc
     1681 cgtcttctcg taatctgcaa atgcctgctc cgtcaccact tccggtgcct caaacgtgtt
     1741 gtgtgcattc atcgttcctg ccacaacact cgcctcgcat acgccgtatg cagcgccgtc
     1801 gtccgtcagc tggatggtca cattctccgc agcttccagt gaattgttgg tcagtgtcac
     1861 cgtgatcacg ccgtccgcgt tctccgatac agactcaatc accttcggca gttcgttctt
     1921 gcctgctcct acggttccag caccgatcag atcgcttctg agaagagttg ctccctgatg
     1981 atgacgatac atatggaata catgataggt cggtgtcttg atcatcttct cgccgtcggt
     2041 cagcatgaca gactggagta cattgatgag ctgtgcgatg catgccatct tcacacggtc
     2101 ggaatgtttg ttgaagatgt tgagtgtcat acctgccaca agtgcatcac gcatggtgtt
     2161 ctgctgatac aaaaatcccg ggttggttcc cggctctacg tccgtccaga ttccccactc
     2221 gtcaacgatc agaccgatct tcttgtccgg atcgtactca tccataatcg caccgtggcg
     2281 gttgatgagt tcgtccatga aataacctct ctttaaggtc tggtagaaaa tctcctccgt
     2341 gaagtctgtt gcgcttccct tcttattcca gtcatcctcc acctcaggaa gtgtataata
     2401 atgaagggaa ag
//
LOCUS       DBMDQJ010000382         2411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15402_length_2411_cov_1.558009, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000382 DBMDQJ010000000
VERSION     DBMDQJ010000382.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15402_length_2411_cov_1.558009"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="ACZO9R_08135"
     CDS             <1..760
                     /locus_tag="ACZO9R_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR0913068.1"
                     /translation="GMENPVVLSKEDWSLGNHQLGFIYDTYDGTTSGSAELIDQLKSG
                     ELKVEDYERFDQFIDTFDLLCEYNINGEDPLGALYDQDPIFLADGDAAIWANGSWAWP
                     NLAEAGADTGDEYGFLPFVLGNDTSDFANNGIEAAATKQLMIDAEQSTEEQQAAAKEF
                     LNWLVYSDNGQRMLVEDAAVIPACSNNTYEALDPLSKDIQTKIGTGKTYSSCFIAPAD
                     HWSVLGASMQKYLGGESTKDELAAEIDAYWTSQE"
     gene            847..1725
                     /locus_tag="ACZO9R_08140"
     CDS             847..1725
                     /locus_tag="ACZO9R_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0913069.1"
                     /translation="MGRKTGEKIKNLCIFALPGCFIFCAVVILPFLYGFYLTLTDWDG
                     VKKVKNLVGVQNYIAVFHDTAFWSSMGLTLKYVLFSVLLVNVVAFLLAYALTNGLKGQ
                     KFFRAGFFTPNLIGGVVLGFIWQFVFSRVLVNFGKSAGWGLFKSSWLADPDKAFWSLV
                     IVTVWQLSGYMMLIYIAGFTGLSSDVLEAASIDGATGFQKMKSIILPLMVPSFVICLF
                     LTLSRAFMVYDVNLTLTGGEPYGSTKLVAMYVYDKAFTSRNYGMGQAEALLLFVIVAC
                     ISGVQIYIGKKKEVEA"
     gene            1725..>2411
                     /locus_tag="ACZO9R_08145"
     CDS             1725..>2411
                     /locus_tag="ACZO9R_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0913070.1"
                     /translation="MARGSKVGGTKRRIKDAVGFLVIIALFLLYMIPFLMVLINSFKQ
                     KRDIIKNPFSLITEKGFTFDNYIKAFEKMQFVKAFGNSLFITGLSTLLVILLASMVAF
                     YFTRAKNTFSKVLFALMAASMIIPFQAIMIPLVSIYGSGLNILNHRLTLVFMHTGFAM
                     SMSVFIYQGFIKSGIPVSLEEAAYLDGCTKVQTFFKVVFPLLKPTTATLVILNVLAFW
                     NDYLLPSLVLR"
BASE COUNT          592 a          530 c          661 g          628 t
ORIGIN      
        1 cggcatggag aacccggtcg tgctctccaa ggaagactgg tcgctgggaa atcatcagct
       61 tggttttatc tatgatacct acgacggaac aacttccgga agtgcggagc tgatcgatca
      121 gttaaagagc ggcgagttaa aggtagagga ttatgagcgt tttgaccagt tcatcgatac
      181 cttcgattta ctctgtgagt acaacatcaa cggagaggat ccgctcggcg cactctacga
      241 tcaggacccg atctttctgg cagacgggga tgcggcgatc tgggcaaacg gttcctgggc
      301 atggccgaac ctggcagagg caggagcgga caccggggat gaatacggat tccttccgtt
      361 cgtactcggc aatgacacct ctgattttgc aaacaacggc attgaggcgg cagcaaccaa
      421 gcagctgatg atcgatgcgg agcagtctac agaggagcag caggcagcag cgaaagaatt
      481 cctgaactgg ctggtatatt ccgataacgg acagagaatg ctcgtggagg atgcggctgt
      541 tattccggca tgcagcaaca atacatacga agcgttagat ccgttaagca aggacattca
      601 gacgaagata ggaaccggaa agacatactc atcctgcttt atcgcaccgg cagatcactg
      661 gagcgtactg ggagcatcca tgcagaagta cctcggcgga gagagcacta aggacgagct
      721 ggcggcagag atcgatgcat attggacttc acaggaataa aaagcggatg cagcagtctg
      781 cataagatgc cttcgggcaa acggggcgtg ccgtaagaca cgcccatata gaaaggaatc
      841 ggagtcatgg gtagaaaaac gggagaaaaa atcaagaatc tgtgcatctt tgcactgccg
      901 ggatgcttca tattctgtgc agtagtgata ctgccattcc tgtatgggtt ttatctgaca
      961 ctgaccgact gggacggtgt caaaaaagta aagaatctgg tcggcgtgca gaattatatt
     1021 gcggtctttc atgacacggc attctggtcg tccatgggac tgacgttaaa atatgtattg
     1081 ttttccgtat tgctggtaaa tgtagtcgcg tttcttctgg catatgcgct taccaacggc
     1141 ttgaaaggac agaaattttt ccgcgcgggc ttttttaccc cgaatctgat cggcggagtc
     1201 gtgctgggat ttatctggca gtttgtattc tcaagagtgc ttgtgaattt tggaaagagt
     1261 gcagggtggg gactgtttaa aagctcctgg ctggcggatc ccgacaaggc attctggtct
     1321 ctggtcattg ttacggtatg gcaattatcc ggctatatga tgttgattta tatcgccggt
     1381 ttcactgggc tgtcttcgga tgtgcttgag gcagccagca tagatggggc gaccggtttt
     1441 cagaaaatga aatccatcat cctgccgctt atggtgccgt cgtttgtcat ctgcctcttc
     1501 ctcacattgt cccgtgcctt tatggtgtac gatgtgaacc tcaccctgac aggcggagaa
     1561 ccgtacggat cgacaaaact ggtggcgatg tacgtgtatg acaaggcatt tacttcacga
     1621 aattacggca tgggtcaggc ggaagcgctg ctgctgtttg tgattgtggc atgcatcagc
     1681 ggtgtccaga tttacatcgg caagaaaaag gaggtggagg cgtaatggca agagggtcaa
     1741 aggtcggcgg aacgaaacgc cgcatcaagg atgcagtcgg ttttctcgtc attatcgcgc
     1801 tgttcctttt atatatgatt ccgtttctga tggtacttat caattcattt aagcagaagc
     1861 gcgacattat caagaatccg ttctcactga ttacggaaaa gggattcaca tttgacaatt
     1921 atatcaaagc ctttgaaaaa atgcagtttg tcaaggcgtt cggcaattcc ctgtttatca
     1981 cgggactgag cacgctgctt gtcatcctgc tggcgtccat ggtggcgttc tattttaccc
     2041 gcgcaaaaaa cacgttttcc aaggtgcttt ttgcattgat ggcggcatcc atgattattc
     2101 cgttccaggc gatcatgatt ccgctggtat cgatttacgg ctcagggtta aatattttaa
     2161 atcacagact gacgcttgtg ttcatgcaca ccgggtttgc gatgagtatg tcggtcttta
     2221 tctatcaggg ctttattaag agcggtatcc cggtctcttt ggaggaggcg gcatatctgg
     2281 atggctgcac aaaggtacag acctttttca aggtggtgtt ccctctgttg aagccgacga
     2341 cggcaacgct cgtgatctta aacgtgctgg cattctggaa tgactatctg cttccgtctc
     2401 tcgtactcag a
//
LOCUS       DBMDQJ010000383         2411 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15403_length_2411_cov_1.299134, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000383 DBMDQJ010000000
VERSION     DBMDQJ010000383.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15403_length_2411_cov_1.299134"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="ACZO9R_08150"
     CDS             complement(<1..893)
                     /locus_tag="ACZO9R_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0913071.1"
                     /translation="MIQVNGLKKAFDGFVALNGVDMHVEKGAIYGLVGPNGAGKSTLI
                     RHLTGVYRPDQGEVQIDGENVYENKAVKSKIAYIPDELFYFMQADTMEMKRFYEGIYP
                     SFDGKLFYRMQEFFPNIDVKRNIRRLSKGMQKQVAFWLAICCKPDILILDEPVDGLDP
                     VMRRQIWSIILSEVAEKEMTVLVSSHNLRELEDVCDHVGIMHHGKIMIERSLSDLQGS
                     VSKIQVACQSGMPKLPEHFQVLHMANTGRVYTMIVKGDPKEAEAALSYCNPTIVDVLP
                     LTLEEIFIYEMGGADYEVKDIL"
     gene            complement(890..1264)
                     /locus_tag="ACZO9R_08155"
     CDS             complement(890..1264)
                     /locus_tag="ACZO9R_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0913072.1"
                     /translation="MIQLNYRDSKPIYEQIKDGFRKLIITNSLSANEKLPSVRELASG
                     LAINPNTIQKAYRDLESEGYTYTVAGKGTFVAEHREILNTRQHELLEEFDEVVEELCF
                     LSVSRSDLITRIEHAAERGEKV"
     gene            complement(1388..2401)
                     /locus_tag="ACZO9R_08160"
     CDS             complement(1388..2401)
                     /locus_tag="ACZO9R_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR0913073.1"
                     /translation="MTKPEQSPWAIDSPSGYTADVADYVMKAYQKLGYEPKLTTKGGV
                     LVALGGKDKKNAVMLEAHIDTLGAMVAEIKSNGRLRVTPVGGMNANNAEAENCRIHTR
                     FGEKVYEGTLQLANASIHVNGDYNDKKRTFDETEIVLDEKVHSREDVEALGIMTGDIV
                     CFDPRTTVTESGYIKSRFLDDKLSVGILLGYARYLKEENVTPERMIYQHITVFEEVGH
                     GGAASIPEGVTEVISVDMGCVGDGLACEETQVSICAKDSHGPYHYDVVTGLIAAAKRE
                     ELDFAVDVYPHYGSDADVALTAGYDVRHGLIGAGVYASHGYERSHVDGVKNTFKLLCA
                     YLG"
BASE COUNT          517 a          729 c          519 g          646 t
ORIGIN      
        1 aggatgtctt tgacttcata atctgcccct cccatctcgt agataaatat ttcttccagt
       61 gtcagcggca gtacatccac aatcgtcgga ttgcaatatg acagtgccgc ctctgcttcc
      121 tttggatctc cctttacgat catggtatac acacgccctg tatttgccat gtgaagcacc
      181 tgaaaatgtt ccggcagctt cggcattccg ctctgacacg ccacctggat tttcgacaca
      241 cttccctgta gatcactcag cgaacgctcg atcatgattt ttccatgatg catgataccg
      301 acatggtcac acacatcctc aagttcacgc agattgtggg acgatacaag cacggtcatc
      361 tctttctctg cgacctccga caatatgatg ctccagatct ggcggcgcat cacagggtcg
      421 agcccgtcca ccggctcatc cagaatcaga atgtccggct tacagcagat ggcgagccag
      481 aatgccacct gtttctgcat tcccttggac agccgtctga tattacgctt tacatcaatg
      541 ttcgggaaaa attcctgcat tctgtagaaa agctttccgt caaacgacgg atagatcccc
      601 tcataaaaac gcttcatctc catcgtgtct gcctgcatga aataaaacag ttcgtctggt
      661 atgtacgcga tttttgattt gaccgcctta ttctcatata cattttcccc gtcgatctgc
      721 acctctccct gatccggacg gtacaccccg gtcagatgac ggatcagcgt tgatttgcct
      781 gctccgttcg gtccgaccag accgtagatg gctccctttt ccacgtgcat gtccacaccg
      841 tttagcgcca caaatccgtc aaatgccttt tttaatccgt ttacctgaat cataccttct
      901 ctcctctctc tgccgcatgc tcgatccgtg tgatcagatc gctcctgctc accgacagga
      961 aacagagctc ctccaccact tcatcgaact cttccagcag ttcatgctgt ctcgtattca
     1021 gaatttccct gtgctccgcc acaaacgttc ccttcccggc aaccgtgtat gtatatccct
     1081 cgctctccag atcgcgatac gctttctgaa tcgtattggg gttgatcgcc agaccggacg
     1141 ccagttcgcg gacagacggc agcttctcgt ttgccgaaag agaatttgtg ataatcagct
     1201 tccgaaagcc atccttgatc tgttcataaa tcggtttgga atcccggtaa tttaactgaa
     1261 tcaaatcata acctcccatc tgtaccaact gtactatttg tcttaataca gctactatag
     1321 catggaaggt tccctcatac aataaagata atcctatgaa attattaact tttatttaac
     1381 ttcggcatca gcccagatat gcacacagga gtttgaaggt gttcttcacg ccgtccacat
     1441 ggcttctctc gtagccgtgc gacgcgtaca cgcccgcgcc gatcagtccg tggcgcacgt
     1501 catagcccgc cgtcagcgcc acatccgcat ccgatccgta atgcgggtag acgtccaccg
     1561 caaagtcaag ttcctcgcgc tttgccgccg caatcagtcc tgtcacaaca tcatagtgat
     1621 acggtccgtg gctgtccttc gcgcagatgg acacctgtgt ctcctcgcag gcaagaccgt
     1681 ctccaacgca gcccatgtca acggaaataa cctcagtcac accctccgga atcgaagcgg
     1741 ctccgccgtg tccgacttcc tcaaagacgg tgatatgctg gtagatcatg cgctccggtg
     1801 tgacattctc ttcctttaaa tatctcgcat agccaagcag gatgccgacg ctaagcttat
     1861 cgtccaggaa acggctcttg atgtagccgc tctctgtgac ggtcgtgcgc ggatcaaagc
     1921 agacgatgtc tcccgtcatg atgccgagtg cctccacgtc ctctctggaa tgtaccttct
     1981 cgtccagcac gatctccgtc tcgtcaaatg tacgcttctt gtcattgtaa tcgccgttga
     2041 catggatgga tgcattggca agctgcaggg ttccctcgta gaccttttcc ccgaagcggg
     2101 tatggattct gcagttctcc gcctcggcat tgttggcgtt cattccgccg accggtgtca
     2161 cgcgcagtct gccgttggac tttatctccg ctaccatggc gccgagcgta tcaatgtgtg
     2221 cctcgagcat cacggcattc tttttgtcct tgccgccaag cgctaccagc acgccgccct
     2281 ttgtggtcag cttcggctca taaccgagct tctggtacgc tttcatcaca taatccgcca
     2341 catctgcggt atatccgctc gggctgtcga ttgcccaggg tgattgttcc ggttttgtca
     2401 agcatcagaa t
//
LOCUS       DBMDQJ010000384         2391 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15559_length_2391_cov_1.444978, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000384 DBMDQJ010000000
VERSION     DBMDQJ010000384.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15559_length_2391_cov_1.444978"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="ACZO9R_08165"
     CDS             <1..546
                     /locus_tag="ACZO9R_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913074.1"
                     /translation="VVQREIGADCASREEALAMALQQDADVILLDGIGNEREAGLLFA
                     AVRRGQFVILAEKEDSVTAALLHLVQCGGSTSGSQILLESLAQGLAGAMAQKLLRRQD
                     EAGRVAAYEVLVADAGVRSLIAENRIQQLDTSAGAEKERGRVQMDDAIYNLYMKSCIT
                     SGTAIAGAKDPEDMRRKVRLF"
     gene            664..1071
                     /locus_tag="ACZO9R_08170"
     CDS             664..1071
                     /locus_tag="ACZO9R_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913075.1"
                     /translation="MREKLQRFMTGRYGADELGRTLNIVVLVSLVVSMFSGLLPVLSV
                     FYWFGIALMIYNCFRMFSKNVSKRYEENQRYLSRRYKAVVKRDQWKKRFAQRDTYRFF
                     KCPGCRQRVRVPKGRGKICITCPKCRREFIRRS"
     gene            1041..1961
                     /locus_tag="ACZO9R_08175"
     CDS             1041..1961
                     /locus_tag="ACZO9R_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5685 family protein"
                     /protein_id="HIR0913076.1"
                     /translation="MQERIHQKKLIWQGKGELGMFGYININQKIMTEENRKAYQAYYC
                     GLCQRLKSNCGAKGQMLLNYDMTFLIVLLTGLYELTNESSEITCALHPTRKRQVWINE
                     ATDYAADMNLIVAYHNLIDDWKDEKAYTKKAFAKMLDKDYARVIEKYPRQVHAVEEFM
                     RKTDEVEARGETNLDVVAGLTGEMLGEIFCWREDEWQEELRTLGFYMGKFIYIMDAYE
                     DYDEDRRKKEYNPLVYAMRESADDMDTLCRLLLTSMMSECAKSFERLPILLHADILRN
                     VLYSGVWSKYEYLQLKKRKAEEKNKKKARK"
     gene            1977..>2391
                     /locus_tag="ACZO9R_08180"
     CDS             1977..>2391
                     /locus_tag="ACZO9R_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HIR0913077.1"
                     /translation="MTDPYQVLGVSRGASDEEIKKAYRALSRKYHPDANVNNPNRDEA
                     EEHFKEVQQAYDQIMKEKQQGGGYGYGGGYSSNSGGYSYAGSHGFGQNESPKMQAAAN
                     YIASRHYVEALNVLNGIPFGERRARWYYFSAIANQG"
BASE COUNT          644 a          462 c          766 g          519 t
ORIGIN      
        1 gttgtgcagc gggagatcgg agcggactgc gcttccaggg aggaagccct tgcgatggcg
       61 ctgcagcagg atgccgatgt gatccttctg gatggaatcg ggaatgagcg ggaggccggg
      121 cttttgtttg cagcagtcag gagagggcag tttgtgattc tggcagagaa ggaggacagt
      181 gtgacggcgg cattgctcca tctggtacag tgcggcggga gcacatccgg ttcgcagatc
      241 cttctggaaa gtctggcgca gggactggcg ggggctatgg cgcagaagct gctgcggcgg
      301 caggatgaag ccgggcgcgt ggctgcctat gaggttctgg tggcggatgc cggggtgcgc
      361 agtctgattg cggagaaccg gatacagcag ctggatacgt ccgccggagc agagaaggag
      421 cgtggcagag tgcagatgga tgatgcgatt tacaatcttt atatgaagag ctgcattacg
      481 tccgggacgg cgatcgcagg agcaaaagat ccggaggata tgaggagaaa ggtgcgcctg
      541 ttttgacagg cagcctgaaa tctaatcaaa gtctaaaatg tttctaaaaa gacgatagga
      601 aggttgacag tctattttgc agggcttaac atgatctcat gatccggaaa ggaaggaatg
      661 gatatgagag aaaaactgca gcgttttatg actggacggt atggagcgga tgagcttggg
      721 agaaccttga acatcgtggt attggtgagt ctggttgtgt cgatgtttag cgggcttctg
      781 ccggtgcttt ccgtttttta ctggtttgga attgcgctga tgatttataa ttgtttccgt
      841 atgttttcaa agaacgtctc gaagcgttac gaggagaatc agcggtatct gagcaggcgc
      901 tacaaagcag ttgtgaagcg ggatcagtgg aagaagcgct tcgcacagag agatacctac
      961 cgctttttca agtgtcctgg atgcaggcag agagtgcggg tgccgaaggg aagaggcaaa
     1021 atctgtatta cctgcccgaa gtgcaggaga gaattcatca gaagaagctg atctggcagg
     1081 gcaaaggaga acttggaatg ttcggttaca taaatatcaa tcagaagatc atgacagagg
     1141 agaaccggaa ggcatatcag gcatattact gcggactgtg ccagagactc aaatccaact
     1201 gtggtgcaaa aggacagatg ctgctcaact atgatatgac atttctgatc gttcttctga
     1261 cgggcttgta tgagcttacg aatgaatcct ccgagattac ctgcgcgctg catccgacca
     1321 ggaagcggca ggtgtggatc aatgaggcga cagattatgc ggcggatatg aatctgattg
     1381 tagcctatca taatctgatt gacgactgga aggacgagaa agcctatacg aagaaggcgt
     1441 ttgcaaagat gctggataag gactatgcgc gcgtgataga gaagtatccg cgccaggttc
     1501 acgcagtgga agagttcatg cgaaaaacag acgaggtgga ggcaagaggc gagacgaacc
     1561 ttgatgtggt ggcaggtctg accggagaga tgctgggtga gattttctgc tggagagagg
     1621 atgagtggca ggaagagctg cgcacacttg gcttttacat gggaaagttc atctacatta
     1681 tggatgccta tgaggattac gatgaggatc gcaggaaaaa ggagtacaat ccactcgtct
     1741 atgcgatgcg ggagagtgcg gatgacatgg atacgctctg ccgtctgctg ctcacgagca
     1801 tgatgtccga gtgcgccaag tcgtttgagc gccttccgat tttattacat gcggatatac
     1861 tgcggaatgt gctgtattcc ggcgtatggt caaaatacga gtatcttcag ctgaaaaaac
     1921 gcaaggcgga agagaagaat aagaaaaaag cgcgcaaata gcgaggttac acatatatga
     1981 cagatccata tcaggtgctc ggcgtatcgc ggggagcctc agatgaagag ataaaaaagg
     2041 catacagagc actcagcaga aaataccatc cagatgcgaa tgtgaacaat ccgaacaggg
     2101 atgaggctga ggagcatttt aaggaagtac agcaggcata tgaccagatc atgaaggaga
     2161 agcagcaggg cggtggatac ggttatggcg gcggctacag cagcaattcc ggcggttaca
     2221 gttacgccgg cagccatgga ttcggtcaga atgaatcgcc gaagatgcag gcggctgcaa
     2281 attatattgc aagcagacac tatgtggagg cgttgaatgt gctgaacgga attccgtttg
     2341 gggaacgccg ggcgcgctgg tactatttca gcgcgattgc caatcagggc a
//
LOCUS       DBMDQJ010000385         2388 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15582_length_2388_cov_1.761259, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000385 DBMDQJ010000000
VERSION     DBMDQJ010000385.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15582_length_2388_cov_1.761259"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(53..970)
                     /locus_tag="ACZO9R_08185"
     CDS             complement(53..970)
                     /locus_tag="ACZO9R_08185"
                     /inference="COORDINATES: protein motif:HMM:NF014081.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR0913078.1"
                     /translation="MEKHRKKLIKNAAVFDGHSSGLAQGKKIVIEDDIVTEITADEVS
                     EENFDEVFDGTDMVAMPGMTDAHVHMGCLFAKGEFPVDYATVLTVAKCKDALMAGFTT
                     FRDAGSVSEGLKLAFDEGVLPGPRIYPCGAYISQMCGHGDSEYAHTCRDIQYRHPSST
                     VLADGVPEIIRAVREQFYRGASHIKLMATEILNRHEDIRLLFGTDMMITGPDYQVEQS
                     EDIRYYEKRFGTMRALRALTGTANEVFALTTYQNPYPKGKIGVLEAGSYADLLLVDGD
                     PTKSVSVLADTDHLRLIMKGGTVYKNTLC"
     gene            complement(1017..1604)
                     /locus_tag="ACZO9R_08190"
     CDS             complement(1017..1604)
                     /locus_tag="ACZO9R_08190"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR0913079.1"
                     /translation="MQKYVISISRQFGSLGRPIAKKLAELLEIEYYDRDIVEEASKVH
                     NIPLREASNLEASAKYLFMSFPLGSGTMATQDALFKTESEMIKKLAVKESCIIVGRCA
                     DAILEDCDHLIRIFIYAPYEDRIVNCVNDLGMDTKAAKRMIRDVDKARDAYHMRYAGY
                     LPNDMNHMDLMVNSATLGVDMTARLLAEVVHDKFS"
     gene            complement(1636..>2388)
                     /locus_tag="ACZO9R_08195"
     CDS             complement(1636..>2388)
                     /locus_tag="ACZO9R_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR0913080.1"
                     /translation="AMAVLEVRNVKKVFGDNVILRGIDLDVQKGDVIVILGPSGSGKT
                     TFLRCLNLLEKPDEGSMVLNGREYNLKNVKNKEKLEIRRNTSFVFQNYNLFVNKTVLS
                     NVTLGLTVGRHMKKEEAEKTGKELLDKVGLAGKYDYYPAQLSGGMQQRVGIARAMAAN
                     PEVILFDEPTSALDPELVGEVLNVMKQFAKQGVTMIVVTHEMQFAREVASQVIFMADG
                     VVVEKGTPEEIFDHPKEETTRKFLKRILKEES"
BASE COUNT          530 a          711 c          470 g          677 t
ORIGIN      
        1 attactacga atctcagcca catgcaagtc agcgtattag cggaataaaa tctcagcaca
       61 gtgtattttt gtacaccgtt ccccctttca taatcaggcg cagatgatcg gtgtctgcaa
      121 gaaccgatac actttttgtc ggatctccat ccaccagaag cagatctgca taggatcccg
      181 cctccagcac accgatcttt cccttcgggt aaggattctg gtaggtcgta agtgcaaaca
      241 cctcgtttgc agtgcccgtc aaagccctca gcgcccgcat tgtgccgaag cgcttttcgt
      301 aataccggat atcctcgctc tgctccacct gataatccgg tcccgtgatc atcatatccg
      361 ttccgaacaa aagcctgata tcctcatgcc ggtttaagat ctcggtagcc atcagtttga
      421 tgtgggatgc accgcggtag aactgctcgc gcactgcccg gatgatctcc ggcaccccat
      481 ccgccaaaac agtgctggac ggatgcctgt actggatgtc gcgacaggta tgcgcatact
      541 ccgaatcccc atgcccgcac atctggctga tgtaggcacc gcagggatag atgcgcggtc
      601 ccggaagcac accctcgtca aacgccagct ttaatccctc gctcacactt cccgcatcac
      661 ggaacgtagt aaacccggcc atcagcgcat ccttacattt tgcgacggtc agcaccgtgg
      721 cgtaatccac cgggaattct ccctttgcga acagacatcc catgtgaaca tgcgcgtccg
      781 tcattcccgg catcgccacc atgtccgtgc cgtcaaatac ctcgtcaaaa ttttcttccg
      841 atacttcatc cgcagtaatc tctgtgacaa tatcgtcctc aatgacaatc tttttccctt
      901 gcgccagccc gctgctgtgc ccatcgaata ccgctgcgtt tttgatcagc ttttttctgt
      961 gtttttccat gtcttatcct ctgtggcact gtgcagtctt acgcacactg cccgtcttat
     1021 gaaaatttat catgcaccac ttccgccaac agtctcgctg tcatatcgac tcccagagtt
     1081 gcgctgttga ccatcagatc catatggttc atatcgttcg gcagataccc cgcataccgc
     1141 atatggtagg catctctcgc cttgtccaca tcacggatca tccgcttcgc cgcctttgtg
     1201 tccatgccga gatcattgac acagttgaca atgcggtctt cgtacggcgc atagataaaa
     1261 attctgatca gatgatcgca atcctccaga attgcgtccg cgcatctgcc gacaatgatg
     1321 caggattcct tcacggcaag tttttttatc atctcgctct ccgtcttaaa cagggcatcc
     1381 tgcgtcgcca tggttccgct gccgagtggg aaactcatga acagatactt tgccgacgcc
     1441 tccagattgc tcgcttccct taacggaatg ttgtgaactt tggatgcctc ctccacaata
     1501 tccctgtcat agtattcaat ttccagaagt tctgccagtt tctttgcaat cggtcttccc
     1561 agacttccga attgtcttga aatcgaaatc acatatttct gcatatcttt ctctcctctc
     1621 cgtcttcgca cacagctatg actcttcctt taaaatacgt tttaagaatt ttctggttgt
     1681 ctcctccttc ggatggtcaa agatctcctc cggcgttcct ttttccacaa ccacaccgtc
     1741 tgccataaaa ataacctggc ttgccacttc tcttgcaaac tgcatctcat gtgttaccac
     1801 aatcattgtg actccctgtt ttgcgaactg tttcatgaca tttaacacct cacccaccag
     1861 ctccggatcg agtgcggacg tcggctcgtc gaaaagaatg acctctggat tcgccgccat
     1921 tgcccgtgca attccgacac gctgctgcat gccgccggat agctgtgccg ggtagtaatc
     1981 gtactttcct gcaagaccga ccttgtctaa aagttctttt ccggtcttct cggcctcttc
     2041 ctttttcata tggcgtccca cggtaagccc cagcgtcaca ttggaaagaa ctgtcttgtt
     2101 cacgaagaga ttgtaattct ggaaaacgaa ggaagtattt ctcctgatct ccagcttttc
     2161 cttatttttt acatttttta agttatattc tcttccgttt aagaccatcg agccttcatc
     2221 cggtttttcc agaagattca ggcagcgcaa aaaggtggtc ttaccggaac cgctcggtcc
     2281 gagtatgacg atgacgtccc ctttctgtac atcaagatcg attcctctta aaatcacatt
     2341 atctccaaaa acctttttta catttctcac ttctaatact gccatagc
//
LOCUS       DBMDQJ010000386         2386 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15594_length_2386_cov_1.558425, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000386 DBMDQJ010000000
VERSION     DBMDQJ010000386.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15594_length_2386_cov_1.558425"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(53..1414)
                     /locus_tag="ACZO9R_08200"
     CDS             complement(53..1414)
                     /locus_tag="ACZO9R_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913081.1"
                     /translation="MQENKMGTMPIDKLIISMSLPIMISMLVQALYNIVDSIFVAQIS
                     ENALTAVSMAFPIQNLMIAVGVGTAVGVNALLARSLGERDFDKVNKIAENAVFLVLLS
                     YLLFLIIGLFFAEPFFRSQTDIEEIVLYGKQYLTVCCCLSFGCFTQLMFERMLQATGK
                     TVYTMITQTTGAVINIILDPILIFGLFGFPKMGITGAAAATVIGQMIAAVLAVFINQT
                     RNKEIQLSLKGFHPDGTIIGQIYAIGVPSIVMQAIGSVMNYGMNRILISFSSTATAVF
                     GVYFKLQSFVFMPAFGLNNGVIPVMAYNYGAGSRERVVKTLRHTVTYAVCIMAVGLLI
                     FQLFPTQLLKMFSASDTMLSLGVPALRIISLSFILAGFGISCSSIFQALGKAIYSMFV
                     SIARQLVALLPVAYFLAQFGNVNLIWWAFPIAELISIIMTILFMFRIFRTIVNKIGEK
                     EMA"
     gene            1595..2032
                     /locus_tag="ACZO9R_08205"
     CDS             1595..2032
                     /locus_tag="ACZO9R_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR0913082.1"
                     /translation="MNILFFLTPKEDVAHVDEDDTLRQVLEKMEHHGYTAIPLLSREG
                     KYIGTITEGDLLWFLKDRNFPDLKLLEDMPITSIQRRRDNEAVKIDESMEDLFGKVMN
                     QNFVPVVDDKRVFIGIVTRKDILAYLGKKAAAQENGVRGMDQA"
     gene            2215..>2386
                     /locus_tag="ACZO9R_08210"
     CDS             2215..>2386
                     /locus_tag="ACZO9R_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR0913083.1"
                     /translation="MNSILSATVLIPLIVIVLLIVLLCVGYVKAPPDMAYIISGVKKK
                     SKVVIGKASIRIP"
BASE COUNT          678 a          512 c          627 g          569 t
ORIGIN      
        1 cgtaatatac aaaacgctat gaaagcttta tttttaggtg gttcttctat ccttacgcca
       61 tctctttttc cccgatctta tttacgatcg tcctaaaaat ccggaacata aagaggatcg
      121 tcatgataat ggagatcagc tctgcgatcg ggaacgccca ccagatcagg ttgacattgc
      181 cgaactgcgc cagaaaataa gcaaccggca gcagtgcaac cagctgtctt gcgatcgaga
      241 caaacatact gtagattgct ttgccgagcg cctggaaaat gctgctgcag gagatgccga
      301 atcctgcaag gataaagctt aagctgataa tccggagcgc cgggacgccg agtgaaagca
      361 tggtatcgga agcgctgaac attttaagca gctgcgttgg gaagagctgg aagatcagaa
      421 gtccgaccgc catgatgcag acggcatagg tgaccgtgtg gcgtaaggtc ttgaccacgc
      481 gctcgcggct gccggcgccg tagttgtatg ccatgaccgg gatgacaccg ttgttgagac
      541 cgaacgcagg cataaacacg aagctctgta atttaaagta aacgccgaac actgccgttg
      601 ccgtggagga gaaactgatc agaatacggt tcataccgta attcatgacc gaaccgatcg
      661 cctgcatgac aatggaaggc acgccgatgg catagatctg accgatgatg gtgccgtccg
      721 ggtgaaagcc ttttaagctc agctgaatct ctttatttct ggtctggtta atgaagacag
      781 cgagaaccgc agcgatcatc tgaccgatga cggtggctgc tgcagcaccg gtgatgccca
      841 ttttcggaaa accaaacaga ccaaagatca agattggatc taagatgatg ttgatgaccg
      901 ctccggtggt ctgggtaatc atggtgtaga cggtttttcc ggttgcctgc agcatacgct
      961 cgaacatcag ctgggtaaag cagccgaagg acaggcagca gcatacggtg agatactgct
     1021 ttccgtagag aacaatttcc tcaatatccg tctggctgcg gaaaaaaggt tccgcgaaaa
     1081 acagaccgat gataaggaac agcagatagc tgagcaatac cagaaaaaca gcgttttccg
     1141 caatcttgtt gactttgtcg aaatccctct ctccgagtga gcgggcgagg agcgcattga
     1201 cgccgaccgc agtacctaca cctacggcaa tcatcagatt ctggatcggg aaagccatgg
     1261 atacggcagt gagtgcgttt tcactgatct gagccacgaa aatactgtcc acgatgttat
     1321 agagagcctg taccagcatg gagatcatga ttggcagaga catggatatg atcaatttat
     1381 cgataggcat tgtgcccatt ttattttcct gcatgggaaa cctcctttgt tgaatggtat
     1441 aacgttaaaa cgacctttct atactaccat tttgcggtag cgattacaag gcatggtgca
     1501 aggattgggg aatcttttgt gaaacggtgg cactgtgccg caatttttgg taaaatagat
     1561 gagaaaaaaa gaacaacagg agattataca gatcatgaat atattatttt ttctgacccc
     1621 gaaggaagat gtggcgcatg tagatgagga cgataccctg cgtcaggttc tggagaagat
     1681 ggagcatcac ggctatacgg caatcccgct gcttagccgc gagggaaaat atatcggaac
     1741 gatcacggag ggcgatttgc tctggttttt aaaggaccgc aattttccgg atttgaagtt
     1801 gcttgaggat atgccgatca cctccatcca gcgccgcagg gacaatgagg cagtcaagat
     1861 tgacgagtcg atggaagacc tcttcggcaa ggtgatgaac cagaacttcg ttcccgtcgt
     1921 ggacgacaag cgtgtgttta ttggtattgt gacgagaaaa gatattctgg cataccttgg
     1981 gaagaaggcc gctgcgcagg agaatggcgt gcggggcatg gatcaggcat agtgatggta
     2041 agccatacgg attatgtgac agagctttct gtcagaatta tcagcaaata agcgagacag
     2101 aacaaaagaa tttcttaaat ttgttatttt tttcacaaaa cagaatggta atccgctata
     2161 atatgaagga gagacaaggc ataacattta tacaaaagaa aggggaattt acaaatgaat
     2221 tcaattttat cagcgacagt attgattcca ctgattgtga tcgtgctgct catcgtactg
     2281 ctgtgcgtcg gatacgtgaa agcaccaccg gatatggcgt acattatttc cggtgtcaag
     2341 aagaagtcca aggtggtcat cggaaaagcg agcattcgca ttccgt
//
LOCUS       DBMDQJ010000387         2385 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15607_length_2385_cov_1.700088, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000387 DBMDQJ010000000
VERSION     DBMDQJ010000387.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15607_length_2385_cov_1.700088"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="ACZO9R_08215"
     CDS             <1..718
                     /locus_tag="ACZO9R_08215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0913084.1"
                     /translation="MKIGAAALACAYRIFLYAYTNRYDPVTNISADTGVLFQIINTLA
                     IFGELTAIMIVFTKDSQEMEYKLVKYNEKLEHMASIDPLTGLYNRWSMRSYLEKIVGK
                     YASGEIGYVSVAIGDIDFFKKTNDTYGHDAGDMVLKELAKVFLEVMGKEGKVCRWGGE
                     EFLFVFQNKNGDQANEVLHDILKRIRGMGIPYQSDWIHVTMTFGLEEYSGEKDMEAVI
                     RAADQKLYRGKENGRNQIVY"
     gene            743..1588
                     /locus_tag="ACZO9R_08220"
     CDS             743..1588
                     /locus_tag="ACZO9R_08220"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0913085.1"
                     /translation="MLTETIAIQVEGSLPGARLCIYIQEKSRELRIKERPLILLCPGG
                     GYEWTSDREAEPMAVQFLAMGYHVAVLRYSCAPAVFPTALLELAEAMKLIHAHAAEWG
                     VKEDQIYVEGCSAGGHLAASLGVFWHESWISERAGVTNEILRPAGLILCYPVITSGEY
                     AHRGSFEALLKGQKTEEMLEKVSLEKQVTEHMPPVFLWHTFTDDCVPVENSLLLIAAM
                     RKVNVPVEFHMYPAGGHGLSTCTEQSMNVDGYGVQEQCQSWLPLVRTWLAEKVRCKTD
                     TNVLG"
     gene            1925..>2385
                     /locus_tag="ACZO9R_08225"
     CDS             1925..>2385
                     /locus_tag="ACZO9R_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter MgtE N-terminal
                     domain-containing protein"
                     /protein_id="HIR0913086.1"
                     /translation="MTEKKKLEDVTEVQMKYQKEIEAIVRGMSSPKVMHDRLLDYHEN
                     DIAAALEDMNPTERQRLYRILNAEEISEVLSYIDEEEIPSYLEEMDMKKKAAVVSEMD
                     ADEIADLLRQMDKKERETLTELLDEEVRHKIDLIDSFDEEQIGSRMSTNYV"
BASE COUNT          666 a          468 c          702 g          549 t
ORIGIN      
        1 aatgaaaatc ggcgctgcag cactggcatg tgcgtaccgt atttttctgt atgcctatac
       61 gaacaggtat gatccggtga caaatatttc tgcggacacc ggtgttctct ttcagattat
      121 caatacgctt gctatttttg gggaattgac ggcaatcatg atcgtgttta ccaaagattc
      181 gcaggagatg gaatacaagc ttgtaaaata taatgagaag ctggaacaca tggcgtccat
      241 tgatccgctt accggcctgt acaaccgctg gagcatgcgg tcgtatctgg agaaaatcgt
      301 cggaaaatat gcgtctggag agatcggata tgtgtccgtt gcaatcggcg acattgattt
      361 tttcaagaag acaaacgata cctacgggca tgatgccggg gatatggtgt tgaaagaact
      421 ggcaaaagtc tttctggagg taatgggaaa agaaggaaaa gtctgccgct ggggcggaga
      481 ggaatttctg tttgtatttc agaataaaaa cggcgatcag gcgaatgagg tacttcacga
      541 tatattgaag agaattcgcg gcatgggcat tccttatcag agtgattgga ttcatgtcac
      601 catgacattt ggactggagg agtatagcgg agagaaggat atggaagcgg tcatccgtgc
      661 agcggatcag aagctctatc gcggaaaaga aaacggacgg aaccagattg tatattaaga
      721 aaacacgggg gagaatagac agatgctgac agaaacaatc gcaatacagg tggagggatc
      781 cctgccgggg gcaagactct gtatctacat tcaggaaaaa agccgggaac ttcggatcaa
      841 ggagcgaccg ctcattctgc tgtgtccggg aggaggatac gaatggacct cagaccggga
      901 ggcggagccg atggcagtgc agtttctcgc aatgggatac catgttgccg tgctgcgtta
      961 ttcctgcgcg ccggcggtgt tcccgacagc gcttttagag ctggcggagg cgatgaaact
     1021 gatccatgca cacgctgcgg aatggggcgt taaggaggat cagatttatg tagaaggatg
     1081 ctccgcggga ggtcatctcg cggcaagttt aggcgtattc tggcatgaat cctggatttc
     1141 tgagagagcc ggagtgacaa atgagattct gcgtccggcg ggactgattc tctgctatcc
     1201 ggttatcacg tccggcgaat acgcacacag gggatcgttt gaagctctgt taaagggaca
     1261 gaagacggag gagatgctgg aaaaagtctc actggagaag caggtgacag agcacatgcc
     1321 gccggtattt ttatggcaca cgtttaccga tgactgtgtg ccggtcgaga attccctgct
     1381 gcttatcgca gcgatgcgca aggtgaatgt gccggtggaa tttcacatgt atccggcagg
     1441 cggacacggg ttatccacct gtacggagca gtctatgaat gtggatggat atggtgtaca
     1501 ggagcagtgt cagagctggc ttccgctagt gcgcacctgg ctggcagaaa aagtgcgttg
     1561 caaaacagat acaaatgtgt taggatagca acggattaaa ataagaatgg aggtggcagc
     1621 atgactgacg acagttgcgg acgaagtaat catattgtct gtgagggaaa aatgagcggc
     1681 agtctgcctg ccattttttg atcgaagaac gattattgag ggtatctttt tttcttatgc
     1741 tctgtattca caggcaatat ttccagacaa cgtaatcaaa tcatgccgaa gacaaggaga
     1801 cagccgtgcg ctgtctgtat cagaatgcca taaatagtgt ggcgttcgtt atttgcgtgc
     1861 acattttgcc ccggcggaat gtgacaacat cggaaaggaa atggtggctt gcatgaaaga
     1921 gcagatgaca gaaaaaaaga aactggaaga tgtgacggaa gttcagatga aatatcaaaa
     1981 agagatcgag gcgattgtcc gcggaatgtc atccccgaag gtgatgcatg acagacttct
     2041 ggattatcat gagaatgata ttgcggcggc gctcgaagac atgaatccca ccgagcggca
     2101 gcgcttatac cgcattttaa atgcggagga gatctcggag gttctctcct acatcgatga
     2161 ggaggagatt ccgtcctatc tggaagagat ggatatgaag aaaaaggcag cagtcgtctc
     2221 ggagatggac gcagatgaaa ttgccgatct gctgcgccag atggacaaaa aagaacgcga
     2281 gacgctgacg gagctgctcg atgaggaggt acggcataag atcgatctca tcgattcttt
     2341 tgatgaagag cagattggaa gccggatgtc caccaactat gtgga
//
LOCUS       DBMDQJ010000388         2384 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15619_length_2384_cov_1.613228, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000388 DBMDQJ010000000
VERSION     DBMDQJ010000388.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15619_length_2384_cov_1.613228"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(240..1670)
                     /gene="rho"
                     /locus_tag="ACZO9R_08230"
     CDS             complement(240..1670)
                     /gene="rho"
                     /locus_tag="ACZO9R_08230"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR0913087.1"
                     /translation="MREKYESLSAVVLRDLAKARGLKNISGMKKSDLVDLMVAEDERQ
                     GREENTKQREQTPQREQAQREQISQREQAQQRPAREPRETREGKGESTAADETISNLD
                     SGIMANGILEVMPDGYGFIRCENYLPGENDVYVSPSQIRKFNLKTGDIVAGNTRVKTQ
                     QEKFSALLYVSSVNGYHPSVAQKRKNFEDLTPIFPNRRIRLERPGCSVAMRIVDLVSP
                     IGKGQRGMIVSQPKAGKTTLLKEIAKSVTKGNPDMHLIILLIDERPEEVTDIKEAIVG
                     ENVEVIYSTFDELPEHHKRVSEMVIERAKRLVEHGKDVMILLDSITRLARAYNLTVPP
                     SGRTLSGGLDPAALHMPKRFFGAARNMREGGSLTILATALVDTGSKMDDVVFEEFKGT
                     GNMELVLDRKLSEKRVFPAIDIVKSGTRRDDLLLEPEEQEAVEIMRKAINGMRTDEAV
                     ENILNMFARTRNNQEYIHMVKKNRIV"
     gene            1907..>2384
                     /locus_tag="ACZO9R_08235"
     CDS             1907..>2384
                     /locus_tag="ACZO9R_08235"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0004473 - malate dehydrogenase
                     (decarboxylating) (NADP+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent malic enzyme"
                     /protein_id="HIR0913088.1"
                     /translation="MTLAEKALLMHEKWNGKLETIAKSKVNSREDLAIAYTPGVAEPC
                     KMIAKDPEAAYKYTMKANTVAVISDGSAVLGLGNIGAYAAMPVMEGKCVLFKEFGDVN
                     AVPICLDTQDTEEIITTIKNIAPAYGGINLEDISAPRCFEIEERLKEMLNIPVFHDD"
BASE COUNT          557 a          679 c          503 g          645 t
ORIGIN      
        1 agacaggctg aaagggttgt tgttatagaa aagacctata tccggtatct gactcctgat
       61 gacattcctg acagaataga ttttcacctc tttataatct tatcgttatt atgttcctgt
      121 ctgtctctat tctcttatgc ccgtatgccc gatccgcata tggcacttac tgcaaagaat
      181 agtcccgaat aaacagcgtt attattttat catggttttt acgaaaaatc aagttctttt
      241 tatacaatcc ggttcttttt caccatatgg atatattcct gattgttgcg cgtgcgggcg
      301 aacatgttca gaatgttctc caccgcctcg tcagtgcgca tgccgttgat cgccttgcgc
      361 atgatctcaa cagcctcctg ctcctccggc tccagcagca gatcgtcgcg ccgcgttccc
      421 gacttcacaa tgtcgatggc agggaacacg cgtttctcgg aaagcttgcg gtcaagcacc
      481 agctccatgt tgccggttcc cttaaactcc tcgaacacca cgtcatccat cttgcttccg
      541 gtatccacca gcgcagtggc aaggattgtg agacttcctc cctcacgcat gtttctggcc
      601 gcaccgaaaa agcgcttcgg catgtgcagc gctgccgggt caagacctcc ggataacgta
      661 cgtccgcttg gcggaaccgt aaggttgtag gctcttgcca gtctcgtgat gctgtcaagc
      721 aggatcatga catcctttcc gtgctccaca aggcgcttgg cccgctcgat caccatctcg
      781 gatacgcgct tgtggtgctc cggcagttca tcaaacgtag aataaatcac ttccacgttc
      841 tccccgacaa tcgcttcctt gatgtccgta acctcctccg gtctctcgtc gatcagcaaa
      901 ataatcagat gcatatccgg atttcccttt gtcactgatt ttgcaatctc ttttaataag
      961 gtggtcttac ctgccttcgg ctgggaaacg atcataccgc gctgtccctt tccgatcggc
     1021 gacaccagat ccacaatgcg cattgcaaca ctgcagcccg ggcgctccaa gcggattctc
     1081 cggttcggga aaatcggcgt cagatcctca aaattcttgc gcttctgcgc gacactcgga
     1141 tgataaccat tgactgatga aacatataac agtgcagaaa acttttcctg ctgcgtcttc
     1201 acacgggtat ttcccgccac gatatcgccc gtcttcagat taaacttgcg aatctgtgac
     1261 ggcgatacat acacatcgtt ctccccgggt agataattct cgcagcggat aaatccatat
     1321 ccgtccggca tgacctctaa gataccgttc gccataattc cgctgtccaa attggagatc
     1381 gtctcatccg ctgcggtgct ctctcccttc ccctctcttg tctctctcgg ctcacgcgcc
     1441 ggccgctgct gcgcctgctc acgctgcgat atctgttccc gctgcgcctg ctcacgctgc
     1501 ggcgtctgct cacgctgctt cgtattctct tctctgccct ggcgctcgtc ctctgcaacc
     1561 atcagatcta ccagatcact ctttttcatg ccggagatat ttttgagtcc tctcgctttt
     1621 gcaagatcac gcaaaacaac ggctgacaag ctctcatact tttctctcat ccattcgctc
     1681 ctgttcttat caatctgcta ttttcctcta tgctctcaat cgtattggga attgtgtctg
     1741 aactgacaaa atgaatttac aatatgcgta ttatacaaca ctttttccaa aataggaagt
     1801 cctaatttcc ttgatttgtc aaaatgataa tggtatgatt tacatattgg tttttataca
     1861 aaaataaact ttatatacag gagacgatat catggagaat cagacaatga cgcttgccga
     1921 gaaggcattg cttatgcatg aaaaatggaa cggaaagctt gagaccatcg caaaatccaa
     1981 agtaaactcc cgcgaggatc tcgccatcgc atacacacct ggcgttgcgg agccatgtaa
     2041 gatgatcgcc aaggatcccg aagctgctta caaatatacc atgaaagcga acaccgtcgc
     2101 cgtcatttcc gacggaagcg ccgttctcgg tctcggcaat atcggcgcat acgccgccat
     2161 gcctgtcatg gagggaaaat gcgttctgtt taaggaattc ggcgatgtca acgccgtacc
     2221 gatctgtctc gatacccagg acacggaaga aatcattacc acaatcaaga acatcgcacc
     2281 cgcatacggc ggcatcaact tagaagacat ctccgcgccg cgctgctttg agattgagga
     2341 gcgcctgaag gaaatgctca acattccggt cttccatgac gacc
//
LOCUS       DBMDQJ010000389         2381 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15650_length_2381_cov_1.127193, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000389 DBMDQJ010000000
VERSION     DBMDQJ010000389.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15650_length_2381_cov_1.127193"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(48..2378)
                     /locus_tag="ACZO9R_08240"
     CDS             complement(48..2378)
                     /locus_tag="ACZO9R_08240"
                     /inference="COORDINATES: protein motif:HMM:NF036578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HIR0913089.1"
                     /translation="MYATACGVYEALINGRKTGDLFMAPGWTSYHHRLQYQTYDITEL
                     LEKDNEITVTVGNGWYKGELGFDARPNIYGDRTALLAMVRIEYEDGETICIGTDTDWH
                     VETGEILFSEIYHGEIQDYTKERRQVGKAVLFEHTDDIGQIVAQESEPVRVTKRFDVK
                     ERIVTPKGELVFDFGQNMAGLVEIRLPKLMEDRLVIRYAETLDKDGNFYTENLRTARC
                     TDTFIYGEKQVGMLVMPHFTFHGFRYICVEGAGTETEASDFTACAMHTDMQPTGTFTS
                     DNTLVNQLQSNIQWGQRGNFLDVPTDCPQRDERLGWTGDAQVFCGTASYNFHTALFFR
                     KWLRDMAAETNPEWGVPHVVPNILGNQAGAAAWSDAATIIPWTIYKVYGDKEMLAEQF
                     PMMKNWVDYIHARVSKNGLWQSGFQYGDWLALDIESGSTDRTGGTDKYLVANAYYAYS
                     TRIVRDAADALGYAEEAKAYGDLYEEIVEAIHVEYVTRSGRMVSETQTACVLMLYFDL
                     IKPEFRDRILETLELNIGAHHNHLTTGFVGTPYLCHCLSENGLHGLADEIFMKEDFPG
                     WLYAVKKGATTIWERWNSILPNGDFDESGMNSLNHYAYGSIGSWLYEKVAGIRCMEPG
                     YKKIRIQPTLTKGMTEVSATYESIYGTIVSAWSCREGKICVDVQIPANTTAVIQLPEK
                     EAEFTVGSGRYHYEYKTDTNLKFEKYSLESTLGELVAEELGRSMFEEMVPGMLEDPLI
                     KFALSMTISDLLMQDAQAKTMYLAVIDALNAANSKL"
BASE COUNT          558 a          671 c          436 g          716 t
ORIGIN      
        1 aaaatatcac attcctaatt gtacgtattt taattttagc ttatttttta taattttgaa
       61 tttgcagcgt tcagcgcatc aatgactgcc agatacattg tctttgcctg cgcatcctgc
      121 ataagcagat cggatatggt catggacaat gcaaacttaa tcagcgggtc ttccagcatt
      181 cccggcacca tttcttcaaa catactgcgg ccaagttctt cggcgaccaa ctctccgagt
      241 gttgactcca acgaatattt ctcgaatttc agattggtat ccgtcttgta ctcgtaatga
      301 tatctgccgg aaccaaccgt aaattccgct tctttctccg gaagctggat aactgccgtt
      361 gtattcgccg gaatctgcac gtccacgcag atttttcctt ctctgcagct ccatgcactt
      421 acgattgttc catatatgga ttcataagtt gcggacacct ctgtcattcc ctttgtcagc
      481 gtcggctgaa tccgtatttt cttatatccc ggttccatac agcgtattcc tgcaactttt
      541 tcgtacagcc agcttccaat cgaaccgtac gcataatggt tcagggagtt catacctgat
      601 tcgtcaaaat ccccatttgg cagaatggaa ttccaacgct cccagattgt cgttgcgcct
      661 ttctttactg catagagcca gcccggaaaa tcctctttca taaagatctc atccgccaac
      721 ccatgaagac cgttttctga caaacaatga cacagatacg gtgtacccac aaaacctgtc
      781 gtcagatgat tatggtgtgc cccgatattc aactccaatg tctccaaaat acgatcacga
      841 aattccggtt ttatcaagtc aaagtacagc atcagcacac aggctgtctg tgtctcgctg
      901 accattcgtc cgcttcttgt cacgtattcc acatgaatgg cttctactat ttcttcatat
      961 aagtcaccgt atgcttttgc ttcctctgca taccccaatg catccgcagc atcccgcaca
     1021 atcctggtcg aatatgcgta ataagcattt gccaccagat atttgtccgt tccgcctgtc
     1081 cggtcggtgc ttccggattc tatgtccagt gccagccagt ctccatactg gaatccactc
     1141 tgccacaatc cgtttttcga gactctcgca tgtatataat ccacccagtt cttcatcatc
     1201 gggaattgct ccgcaagcat ctctttatcc ccgtaaacct tataaatcgt ccacggaatg
     1261 attgtcgcag catcgctcca tgccgctgct cccgcctgat ttccgagaat attcggaaca
     1321 acatgaggta cgccccactc cggattggtc tctgccgcca tatcccgcag ccattttcgg
     1381 aaaaatagtg ctgtgtgaaa attgtaggag gctgtaccac agaatacctg tgcatctccg
     1441 gtccagccca accgttcatc tctctgtggg cagtctgtcg gaacatcgag gaagttgcct
     1501 ctctgtcccc actgtatatt actctgcagt tggttaacca acgtgttgtc ggatgtgaat
     1561 gttcctgtcg gctgcatgtc tgtgtgcatt gcacatgccg taaaatccga tgcctctgtt
     1621 tccgttcctg cgccttccac acaaatataa cggaatccgt ggaaagtgaa gtgcggcatc
     1681 acaagcattc ccacctgctt ttctccgtat ataaatgtat ctgtacatct tgccgtccgc
     1741 agattctctg tataaaagtt tccatcttta tccagtgttt ctgcatatcg aataactaat
     1801 ctgtcttcca taagttttgg aagtcgtatt tctaccagtc ctgccatatt ctgtccgaaa
     1861 tcaaacacca attctccttt tggagtcacg atcctttcct ttacatcaaa ccgtttcgtt
     1921 acccggaccg gctcggattc ctgcgccaca atctgcccga tatcatccgt atgttcaaac
     1981 agaactgcct ttcccacctg ccttctttct tttgtataat cctgtatctc accgtgataa
     2041 atctcggaaa acagaatttc tcctgtttcc acatgccagt ccgtatcagt gccgatgcag
     2101 atagtttccc cgtcttcgta ctcgatacgc accattgcca gcaaagccgt tctgtcgccg
     2161 taaatattcg gtcttgcatc aaacccgagt tctcccttat accacccgtt tcctacagta
     2221 accgtaatct cattatcttt ttccaggagt tccgtaatat cataagtctg atactgcagt
     2281 cggtgatgat aagatgtcca gcccggagcc atgaacaagt ctcctgtttt ccttccgtta
     2341 ataagtgcct catatacacc acaggcggtc gcatacagcc t
//
LOCUS       DBMDQJ010000390         2371 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15719_length_2371_cov_1.478414, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000390 DBMDQJ010000000
VERSION     DBMDQJ010000390.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15719_length_2371_cov_1.478414"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="ACZO9R_08245"
     CDS             <1..670
                     /locus_tag="ACZO9R_08245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0913090.1"
                     /translation="YVGAMRNAYRMFAGLYNEVSHGMSVLTPDYRVAPEHPYPAALED
                     AVCAYEWLLAQGWFSEQIIVAGDSAGGGLAMALCHYLINHGRQLPCGIIAMSPWTDLT
                     ASGESYDTNYERDPLFGKTRDSLIYNRDYVGDHDPMDEYISPMFGDFRGFPPMLIQVG
                     SYEMLLSDSVDVAAKARGQGVKVRLSIYDGMFHIFQMAAKMLPESRKAWVEIGKFIEI
                     LLRE"
     gene            complement(682..1920)
                     /locus_tag="ACZO9R_08250"
     CDS             complement(682..1920)
                     /locus_tag="ACZO9R_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR0913091.1"
                     /translation="MNQKNCMVAQSGGPTVAINASLAGVIDGVSASSMYDTVYGSLNG
                     ITGILNDDLLNLTELMDGHPEYLSRLAQTPAMYLGSCRYKLPHYLDDDSPYVFIFKQF
                     ETYHIEAFFYIGGNDSMDTVLKLSEYGKRIGSSVRIIGIPKTIDNDLCETDHTPGFGS
                     AAKYIASSLLEIAHDTFIYAVKSVTIVEIMGRDAGWLTAAAALARNGYNTAPHLIYLP
                     EVAFDPEDFLLDVKRLLFERNNVIVAVSEGIRDASGNYISASEQSIDTFGHSQLSGAG
                     KTLEFLVKEKLGVKVRSVEINVLQRCAAHLASQTDLGESFALGKKAVALSEEGVTASM
                     VTIERTSNAPYTVDYSYAEIRQIANEAKSVPREWINSAGNDIEQPLLDYLAPLILGES
                     QVSYHNGLPVYMDVSHLSKH"
     gene            2056..>2371
                     /locus_tag="ACZO9R_08255"
     CDS             2056..>2371
                     /locus_tag="ACZO9R_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HIR0913092.1"
                     /translation="MKMCTLCPRNCAVDRAGGERGICGQTEQLKVARAALHYWEEPCI
                     SGESGSGAVFFSGCALHCVFCQNRSIANGTAGKEITKERLAEIFLELQEKGANNINLV
                     TPG"
BASE COUNT          569 a          551 c          718 g          533 t
ORIGIN      
        1 atatgtcggt gcgatgcgca atgcataccg catgtttgcc ggattgtaca atgaggtcag
       61 ccacggcatg agcgtgctca cgccggatta ccgcgtggca ccggagcatc cgtatccggc
      121 ggcgctcgag gatgccgtgt gcgcgtatga atggctactt gcgcagggat ggttttcgga
      181 gcagatcatt gtggcgggag attcggcggg cggcggactt gcgatggcac tctgccacta
      241 tctgatcaat cacggcagac agcttccgtg cggaatcatt gccatgtcgc cgtggacgga
      301 tctgacggca agcggggagt cttacgatac caactacgag agggatcccc tgtttggaaa
      361 gaccagggac agcctgatct acaatcggga ttatgtggga gaccacgacc cgatggatga
      421 atatatatca ccgatgtttg gcgatttcag aggatttccg ccgatgctga tacaggtggg
      481 atcgtatgag atgctgttgt cggattctgt ggatgtcgcc gcgaaggcga gaggacaggg
      541 cgtaaaagta cgcttaagta tctatgacgg gatgttccac attttccaga tggcagcaaa
      601 gatgctgccg gaatccagaa aagcgtgggt cgagatcggt aaatttatcg agattctgct
      661 ccgggaatag ggagcacctg tctagtgctt agacaggtgg ctcacatcca tgtatacagg
      721 cagaccgttg tgataggaga cctgcgattc gccgagaatc agcggagcaa gatagtcgag
      781 cagaggctgt tcgatgtcgt tgccggcact gttgatccac tctctcggaa cgctctttgc
      841 ctcgttggcg atctggcgga tttccgcata ggagtaatcc acggtatacg gtgcgtttga
      901 agtgcgctcg atggtgacca tggaagctgt cacaccctcc tcggagagtg cgacggcttt
      961 tttgccgagt gcgaaagatt cgcctaagtc tgtctgggaa gccagatgtg cggcacagcg
     1021 ctgcaggaca ttgatttcca cggaacgcac cttgacacca agcttttcct tgacaaggaa
     1081 ctcaagtgtc ttgccggcgc cgcttaactg actgtgaccg aacgtgtcga tggactgctc
     1141 ggacgcgctg atgtaattgc cggaggcgtc gcggatgccc tcggacactg cgacgatgac
     1201 attgttgcgc tcgaaaagga gacgcttgac gtctaaaagg aaatcctccg ggtcgaaagc
     1261 gacttccggc agatagatta agtgcggggc ggtgttgtat ccgttgcgcg cgagtgccgc
     1321 ggcagcggtc agccatcctg cgtcgcgtcc catgatctca acgatggtca cgcttttgac
     1381 cgcgtagata aaggtgtcgt gcgcgatctc taagagcgag gaggcaatgt attttgcggc
     1441 agagccaaag cccggggtgt gatcggtctc gcagagatca ttgtcgatgg tcttcgggat
     1501 gccgataatg cggacggaac tgccgatgcg ttttccgtac tcggacagct ttaacacggt
     1561 gtccatggaa tcattgccgc cgatatagaa aaacgcttca atgtggtaag tttcaaactg
     1621 cttaaatatg aagacatagg gtgaatcatc atccagataa tggggaagtt tgtagcggca
     1681 ggagcctaag tacatcgcag gtgtctgcgc aaggcgggaa agatattccg gatgaccgtc
     1741 cataagctcc gtgaggttga gaaggtcatc gtttaaaata ccggtgattc cgttgagcga
     1801 gccgtaaacg gtgtcgtaca tggaagaggc ggacacgccg tcaatgacac cggcaagact
     1861 tgcgttgatc gcaacggtag gaccgccgga ctgggctacc atacaatttt tctgattcat
     1921 aacgaactcc ttatgtgtaa atatatcaat gtcagattgt tccgaaaatg gtcatttcgg
     1981 aaatcaaaca ttatcatacc caaaatacgc gggattatca agattgaatt tagatagaaa
     2041 gagacggacg gaaagatgaa aatgtgtaca ttgtgtccta gaaactgcgc agtggaccgt
     2101 gcgggcggag agcgcggcat ctgcggacag accgaacagc tgaaggttgc gcgtgcggcg
     2161 cttcattact gggaggaacc ctgcatctcc ggggaaagcg gctccggcgc ggtatttttc
     2221 tccggctgtg ccctgcattg tgtgttctgc cagaaccgca gcatcgcaaa cgggacggca
     2281 ggaaaggaga ttaccaagga gcgtctggca gaaatattcc tggaactgca ggaaaaaggg
     2341 gcgaataata tcaatctggt gacgccggga c
//
LOCUS       DBMDQJ010000391         2370 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15725_length_2370_cov_2.165712, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000391 DBMDQJ010000000
VERSION     DBMDQJ010000391.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15725_length_2370_cov_2.165712"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="ACZO9R_08260"
     CDS             complement(<1..289)
                     /locus_tag="ACZO9R_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913093.1"
                     /translation="MLRMFWYEIKMDFLRSIRYRFGMISDLLVYFALFTIFMVTDSGN
                     SYAQTYHYGNYKELVLLGYVAWIYAISAISNVAGSLQSELTHGTLYKKVSSK"
     gene            complement(282..701)
                     /locus_tag="ACZO9R_08265"
     CDS             complement(282..701)
                     /locus_tag="ACZO9R_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012235.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR0913094.1"
                     /translation="MSEKSRKSTADAAIDRGKEKFPIYETVKNKLVETLSYGQKRIVS
                     LMSAVVTGAKCVIIDEATDGLDIDNRKLVADTIRSVRDGRSIIVIAHDFSFASDVADT
                     LIFLKDGRFAEVVENARGEEIEQIYKKLYHGEVRTDA"
     gene            802..2205
                     /locus_tag="ACZO9R_08270"
     CDS             802..2205
                     /locus_tag="ACZO9R_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913095.1"
                     /translation="MKIRRKHPVRVNHLGSDPIPQLVRRLALPAMLAQFVNVLYSIID
                     RMYIGNIANVGDIALAGVGICGPIVTMISAFAAWIGNGGAPLLSIKSGEQNKEGASAI
                     LANCFVLLIGMAVCLTIGAYLCKDALLVWFGATERIFPYAETYMRIYLIGTVFAVLSL
                     GLNQFIICQGFSNLGMVSVIIGAILNILLDPLFIFVLHMGVRGAAVATVLSQAASCAF
                     SLYILFRGPVPVKITFRGYSLPVCRDVLTLGLTPFLIILFDNVLIIVQNVMLKNYGGA
                     DSDMFLTCNTIVQSFMLIITMPLSGISGGTQSVLGYNYGAGDTARIRLAEKHILKTAL
                     LFCTIMFVFAQWDVSAGIFVGLFTQNAAYRSMTVHFIHIYTLSVIPLAVQYALVDGLT
                     GMSLVGASLPLSFFRKGIYIALAVFLPIRFGAPAMFYAEPLSDVVAAVVTTIVFVTVV
                     PGLLRKREALLGKHIEK"
BASE COUNT          536 a          662 c          547 g          625 t
ORIGIN      
        1 attttgagga taccttcttg tacaaggttc cgtgtgtcag ctcactctgc agcgatcccg
       61 cgacattgga aattgctgag attgcataga tccaggcaac atatcccaaa agcaccagtt
      121 ccttgtaatt tccataatga taggtttgtg cgtaagaatt tccggaatcc gtcaccataa
      181 atatcgtaaa caaagcgaaa tagaccaata agtcggaaat cattccaaac cggtagcgga
      241 tcgaccgcag gaaatccatt tttatctcat accagaacat tctaagcatc tgtccgcacc
      301 tccccatgat acagtttctt ataaatctgc tcgatttctt cccccctggc gttctccacg
      361 acttctgcaa agcgcccgtc ctttaggaaa ataagcgtat ctgcgacatc cgatgcaaat
      421 gagaaatcat gcgcaatgac aatgatactt ctgccatcac ggacactgcg tatggtatcc
      481 gcaaccagct ttcggttatc aatatccagt ccgtccgtcg cctcgtcaat aatcacgcat
      541 tttgcgcctg tcacaaccgc agacatcagc gatacgatgc gcttctgccc gtacgagagc
      601 gtttcgacca gtttattttt caccgtctca tagattggga atttctcttt tccccggtca
      661 atcgcggcat ccgccgtact ttttcttgac ttttcggaca tttgattcta atatcttcct
      721 gcatgctttg atatatgata gaagaaactc ttttttcagg gatttttcgt gtgaaataga
      781 gaatacaaaa gaggtgccaa tgtgaaaatc aggagaaaac atccggtcag agtcaatcat
      841 ctcgggagcg atcccatccc acagctggtg cgccggcttg cccttccggc aatgctcgcc
      901 cagtttgtca atgtactcta cagcattatc gaccgcatgt atatcggaaa tattgcaaac
      961 gtcggggaca ttgcgctcgc gggcgtggga atctgcggac cgatcgtgac gatgatctcc
     1021 gcgtttgccg cctggatcgg gaacggtgga gcgccgttac tctccatcaa atccggtgaa
     1081 cagaataagg agggcgcttc cgccatcctc gccaactgct ttgtgcttct gatcggcatg
     1141 gcagtctgcc tgaccatcgg agcgtatctg tgcaaggacg ccctgctggt atggttcggc
     1201 gcgacggagc gcatttttcc ctacgcagaa acctacatgc gcatttacct gatcggaacc
     1261 gtcttcgccg tgctctcgct cggactgaac cagttcatca tctgtcaggg attttccaat
     1321 ctgggcatgg tctccgtcat catcggcgcc attttaaata ttctgctcga cccgctcttt
     1381 atcttcgtcc tgcacatggg tgtgcgcgga gcggccgtcg ccaccgtgct ctcacaggcg
     1441 gcttcctgcg cgttttcgct gtacattctg ttccgcggtc ctgtgccggt gaaaatcacg
     1501 ttccgcggct actccctccc ggtgtgccgc gatgtcctta cgctgggact gacgccgttt
     1561 ttaatcatcc tgttcgacaa tgtgctgatc atcgtccaga atgtcatgtt gaaaaattac
     1621 ggcggcgccg acagcgacat gttcttaacc tgcaacacga ttgtgcagag cttcatgctg
     1681 attatcacaa tgccgctcag cggaatctcc ggcgggacac agtcggtgct cggctacaac
     1741 tacggcgccg gtgacaccgc ccgcatccgc cttgccgaga agcatatttt aaagacggcg
     1801 ctgcttttct gcaccatcat gttcgttttt gcccagtggg atgtcagtgc aggcattttc
     1861 gtcggattat ttacgcaaaa tgccgcttac agatctatga cggtgcattt tatccacatc
     1921 tacacgctct ccgtcatacc acttgccgta caatatgcgc tcgttgacgg gctgaccggc
     1981 atgagcctcg ttggcgcctc gctgccgctt tccttcttcc gcaaagggat ctacattgcg
     2041 ctggcagtct ttttgccgat tcgctttggc gcaccggcga tgttctatgc cgagccgctc
     2101 tccgatgtcg ttgccgccgt cgtcacgacg atcgtctttg tgaccgtcgt ccccggactc
     2161 ctccgcaaaa gagaggcgct cctcggcaag cacatagaaa agtaaaaagg aacgcctgtc
     2221 agaaaatgga actgtcgcaa taagcatacc gtactttgct ctgataagta atattataag
     2281 aaatagatac ttaaaagttc cagataaaca tgcggcggca aattctgacc gcaaaatccg
     2341 gtatttctaa ccatgccgca gctggcatcc
//
LOCUS       DBMDQJ010000392         2359 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15804_length_2359_cov_1.806023, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000392 DBMDQJ010000000
VERSION     DBMDQJ010000392.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15804_length_2359_cov_1.806023"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(59..1861)
                     /gene="uidA"
                     /locus_tag="ACZO9R_08275"
     CDS             complement(59..1861)
                     /gene="uidA"
                     /locus_tag="ACZO9R_08275"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002594455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HIR0913096.1"
                     /translation="MLYPRENSVRGVIDLGGVWNFKLGDEKEPGDSFAMGEDTELMAV
                     PASYNDQKDDPKYRNHYGWAYYERKITVPSYLKGQRLVLRFDAVTHAAKVYLNGRFLT
                     AHKGGFLPFEVDVTDQLVPGETAELVVAADNRVNHSTLPVGNEGSTAFFGSDNPGVPS
                     VEAAKVWRKPQNLPNFDFFNYAGINRPVRIYTTPAAYIEDITLVPDIDGDTGVVNYEI
                     KTAGSVCEDEVRIRILDADGNEVAAADGAGGTVRIPDARLWWPWPGEPYLYTASVTYG
                     EDLYEETFGIRTVAVEGTQFLINGKPFYFKGFGKHEDAAFRGRGLDVCLDVKDINLIH
                     WLNANSFRTSHYPYAEEMYQLCDREGIVVIDETPAVGIGAGDKIDPYKTFHIREHHEQ
                     VLREMIARDKNHPCVVMWSMGNEPDTEHFPESAYDYWHSLYELTHSIDPADRPVTFVC
                     CQNNYERDLVTRTMDVVCVNRYYGWYNLSGDLDAACYALNMELDFWEKQNKPVMITEY
                     GADAVAGIHESVPEMFSEEFQKEYYRRQNAEFDKRSFFIGEQVWNFADFATIQGCMRA
                     DGNKKGLFTRDRRPKMAAHYFKERWGQIPNFEYK"
     gene            complement(1922..>2359)
                     /locus_tag="ACZO9R_08280"
     CDS             complement(1922..>2359)
                     /locus_tag="ACZO9R_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase-related protein"
                     /protein_id="HIR0913097.1"
                     /translation="VIEFLEKRGFSCGVMLSYYLLLALARRGRYESVYRLLLNDSDHG
                     WCNMLREGATTCFEAWGKDQKWNTSLCHPWASAPVPVILEEIAGIHLSPEGGCDFAPH
                     IPKEVDYFHTSVRMRGKTYTVTKQDGEIHAAIDGIEQPKADAK"
BASE COUNT          500 a          743 c          514 g          602 t
ORIGIN      
        1 aaatctgacc atggagaaat ggaagacctt tcccactgac atacaggcag gaggtccttt
       61 atttatactc aaaattcgga atctgtcccc agcgctcctt aaaatagtgt gccgccatct
      121 tcggccgccg gtctctggta aagagacctt ttttatttcc gtcggcgcgc atgcagccct
      181 ggattgtggc gaaatccgca aaattccaga cctgctcgcc gatgaagaag ctgcgcttgt
      241 caaattccgc attctgtctg cggtaatatt ccttctggaa ctcctcggag aacatttccg
      301 gcacgctctc gtggatcccc gcaaccgcat ccgcgccgta ttctgtaatc atcaccggtt
      361 tgttctgttt ttcccagaaa tccagttcca tatttaatgc gtagcatgcc gcgtcaagat
      421 cgccggacag attgtaccag ccatagtaac ggttcacgca caccacgtcc atcgtcctcg
      481 tcacgagatc tctctcgtag ttgttctggc agcacacgaa ggttaccgga cggtctgccg
      541 gatcaatgct gtgcgtcagt tcatagagcg agtgccagta atcgtacgcc gactccggaa
      601 aatgctcggt gtccggttca tttcccatcg accacatgac tacgcacgga tgattcttgt
      661 cccttgcaat catctcgcgt agcacctgct cgtggtgctc tctgatgtgg aaggtcttgt
      721 acgggtcgat cttatctccc gcgccgattc cgaccgcagg cgtctcatcg atcacaacga
      781 taccttccct gtcgcacagc tgatacatct cctccgcata cggatagtgg ctggtgcgga
      841 aagaattggc attcagccag tggatcagat tgatatcctt gacatccagg cacacatcga
      901 gtcctcttcc gcggaaagcc gcatcctcgt gttttccaaa gcccttaaaa tagaacggtt
      961 ttccattgat caaaaactgc gttccctcta ccgccaccgt gcggatgccg aatgtctcct
     1021 cgtagagatc ctccccgtaa gtgacactcg ccgtatagag gtacggctcc cccggccacg
     1081 gccaccacag tctggcatcc gggattctga ctgttccgcc cgctccatcc gccgctgcca
     1141 cctcattgcc gtccgcgtcc agaatacgga tgcgcacctc atcctcacat acgctgcccg
     1201 ccgtcttaat ctcataattt accactccgg tatcgccgtc aatatccggc accagcgtaa
     1261 tgtcctcaat ataagccgcc ggcgtcgtat agatgcggac cggacggttg attcccgcat
     1321 aattaaagaa atcaaaattc ggcagattct gcggttttct ccataccttc gccgcttcaa
     1381 cactcggcac gcccggattg tccgatccga aaaatgccgt gctgccctca tttccgaccg
     1441 gaagcgtgct gtgatttacc cggttgtccg ccgcaacgac gagttccgcc gtctctcccg
     1501 gcacaagctg atctgtcaca tccacctcaa acggaagaaa gccaccttta tgcgcagtca
     1561 gaaatctgcc atttaagtac acctttgcag catgtgtaac cgcgtcaaag cggagcacga
     1621 gacgctgtcc cttcagatag gacggaaccg taatcttccg ctcgtaatac gcccagccgt
     1681 aatgattccg gtatttcggg tcatctttct gatcgttgta cgatgccggt accgccatca
     1741 gttcagtgtc ctcccccatc gcaaaggagt ctcccggctc cttctcatct cccagtttaa
     1801 aattccatac tccgccgaga tctatcactc cgcgcacgct gttttctctt ggatacaaca
     1861 tcttttcttc ctccattctt tctgctgatt tcttcccgat agcttttgtc cgggatttct
     1921 tttattttgc atctgctttt ggctgctcta tgccgtctat cgcagcatgg atttccccgt
     1981 cctgcttcgt aacggtatac gttttcccac gcatccgcac ggacgtatgg aagtaatcca
     2041 cttcctttgg aatatgcggt gcaaaatcac agccgccttc cggcgacaga tggattccgg
     2101 cgatctcctc taagatcaca ggcacgggcg cactcgccca cggatgacac aggctcgtgt
     2161 tccacttctg atctttcccc cacgcctcaa agcaggtcgt cgctccctcc cgcagcatgt
     2221 tgcaccatcc gtgatcgctg tcgtttagca gcagcctgta cacactttca taccgccccc
     2281 gccgtgccag tgcaagcagc aggtaataac ttaacatcac gccgcaggaa aatccccgtt
     2341 tttccagaaa ctcaatgac
//
LOCUS       DBMDQJ010000393         2358 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15818_length_2358_cov_1.293310, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000393 DBMDQJ010000000
VERSION     DBMDQJ010000393.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15818_length_2358_cov_1.293310"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZO9R_08285"
     CDS             complement(<1..207)
                     /locus_tag="ACZO9R_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HIR0913098.1"
                     /translation="MKVCYKAGAVTAQNHYYSIAADAVEIRVWFLTDDITRIRAGFDG
                     DWDEASYSLVTTAWESRTDELMKDY"
     gene            427..1248
                     /locus_tag="ACZO9R_08290"
     CDS             427..1248
                     /locus_tag="ACZO9R_08290"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0913099.1"
                     /translation="MIDVAQTSMAAGAACAIAQVTGVNDNMSQSHYHDYFELYFLDSG
                     ERYHLMDDKLFKLQAGDCILFPPQTMHRSYGDQDMAFSRVVLYFRPKTITSADLLHKL
                     LHSQVVYRPDSQEIHQLRRIIYAMEEEQHSHLDCHDECMQSLLNLIMVQLLRMNQEST
                     GILRETRVTKVIQYIHNHYAEDITLKDLSERFYVSEYYLCREFKKSTKRTIVEYIKQT
                     RIMNAKRLFMETDRNVTEVARETGFSNLTHFNRVFREVADVTPSEYRKRCQELHR"
     gene            complement(1395..1955)
                     /locus_tag="ACZO9R_08295"
     CDS             complement(1395..1955)
                     /locus_tag="ACZO9R_08295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR0913100.1"
                     /translation="MAEGKRREERRRNMQRGQGYGMNSIFHRISVRKYQNRDVEQEKI
                     ELMLKAAMAAPSACNQQPWEFYVVKDKAVIERLSEASPYAKCAKDAPVVFVPCFRSKG
                     IAPEYFDIDMSAAVENLLLEADELGLGAVWMGISPDEGRMKAVREVLDIPEDLHAFAL
                     VPCGYPAEERAQEDRYEESRVHYIMN"
     gene            complement(2102..2329)
                     /locus_tag="ACZO9R_08300"
     CDS             complement(2102..2329)
                     /locus_tag="ACZO9R_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HIR0913101.1"
                     /translation="MTTTKLFKKKIPAQTYDKDSQKPVIKASICNGEQIAGFKDLHTG
                     KIEEVMLIKGPADLERFKKMYGIEDEIGKEY"
BASE COUNT          518 a          684 c          483 g          673 t
ORIGIN      
        1 gtaatccttc atcagttcat ctgtgcggga ttcccacgcg gttgttacca gactgtagga
       61 cgcttcatcc cagtctccgt caaagcccgc ccggatacgg gtgatatcat ccgtaaggaa
      121 ccatacacga atctccactg catctgcagc aatcgaataa taatgattct gtgctgtgac
      181 agcacctgct ttgtaacata ctttcacgag ttttcctcct ttggttttgt aactcttttt
      241 cttattgtct atatccggtt cattcagatc tgttttcctt ctgtcatcat cttacaaaac
      301 ctcaggttca ataactatac agactttgtt caaaatcgca ctttttttta catatcttgc
      361 acttgttgca ttttcgcttt ataatttcta tagaaacaga gttttttatt ttctatgagg
      421 tgccttatga ttgatgttgc acagacaagt atggcagccg gtgcagcctg cgccatcgcc
      481 caggtaaccg gcgtcaatga caatatgtcg cagtctcact atcacgatta ttttgagctg
      541 tattttctcg acagcgggga gcgctatcat ctgatggatg acaagctctt caagcttcag
      601 gccggggact gtattctctt ccctccacag acgatgcacc gttcctacgg tgaccaggat
      661 atggcatttt cgcgggtggt gctgtatttc cgcccgaaga cgatcacctc ggcggatctt
      721 cttcataagc ttctccactc gcaggtggtt taccgtcctg acagtcagga gatccaccag
      781 ctgcggcgta tcatctatgc catggaagag gagcagcatt cgcatctgga ctgccacgac
      841 gaatgtatgc agtctctttt gaacctgatt atggtgcagc ttctgcgcat gaatcaggag
      901 agcaccggca tcctccgcga gacccgggtt accaaagtga ttcagtatat ccataaccat
      961 tacgcagaag atatcacact caaagacctc tccgagcgtt tttatgtcag cgaatattac
     1021 ctctgccggg aattcaagaa aagtaccaag cgcacgatcg tggagtatat caagcagacc
     1081 cgcatcatga acgccaagcg gctcttcatg gaaacggacc ggaatgtcac cgaggtcgcc
     1141 agagaaaccg ggttttccaa cctgacacat tttaaccggg tcttccggga agtcgcggat
     1201 gttacaccgt ccgagtaccg gaaacgctgt caggagctgc accgctgacg cgtcgccaag
     1261 cagggggctt gttttccctc ttatttttca taaatatacg aaaacggggc ggcagagaaa
     1321 catttccctg ccgccccgtt tttatcgcgt aacatctgtg cttgcaaatc ttctgccatt
     1381 ggaatattgg caaatcagtt cataatatag tgcactctgc tctcctcata ccggtcttcc
     1441 tgcgctctct cctctgccgg gtaaccgcag ggaaccagcg caaatgcatg aaggtcctcg
     1501 ggaatatcca gcacttcccg caccgctttc attctgcctt catccggcga aattcccatc
     1561 cagaccgcgc caagcccaag ctcgtccgcc tccagcagaa ggttctccac cgccgcgctc
     1621 atatcgatat caaaatattc cggcgcaatc cccttagacc ggaagcacgg cacaaacacg
     1681 accggcgcgt ccttcgcaca ttttgcataa ggcgaagcct ctgacagccg ctcgatcacc
     1741 gccttgtcct tcaccacata aaattcccac ggctgctgat tgcacgcgga cggagccgcc
     1801 atcgccgctt ttaacatcag ctcgattttc tcctgctcca cgtccctgtt ctgatatttt
     1861 ctaacgctga ttctgtgaaa aatactgttc atcccgtatc cctgtcctct ctgcatgttc
     1921 cgccggcgtt cttcccttct cttaccttct gccatgcgca gctgtctctg ccgcgctctg
     1981 tcttatgtcc ggtactccat gttccggctg tccatacttt ttatctgtaa tcttccgaca
     2041 cttccatgta ctcccggctg cgatactcct cgtttagttc ccgcaattcc ttttcgccat
     2101 atcaatactc ctttccgatc tcatcctcaa tgccatacat tttcttaaac cgttccaaat
     2161 ccgccggccc cttaatcagc atcacttcct cgatctttcc cgtatgaaga tccttgaaac
     2221 cggcaatctg ctcgccgttg cagatgctcg ccttaatcac cggcttttgg ctgtctttat
     2281 cataagtctg tgcgggaatt tttttcttaa acagtttcgt tgtggtcacc ttctttcacg
     2341 tggaatgatt atgtctat
//
LOCUS       DBMDQJ010000394         2355 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15837_length_2355_cov_1.364685, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000394 DBMDQJ010000000
VERSION     DBMDQJ010000394.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15837_length_2355_cov_1.364685"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(213..2033)
                     /gene="glmS"
                     /locus_tag="ACZO9R_08305"
     CDS             complement(213..2033)
                     /gene="glmS"
                     /locus_tag="ACZO9R_08305"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR0913102.1"
                     /translation="MCGIIGYTGSEDVREVLLDALELLEYRGYDSAGIALRDEESGKT
                     EVRKCAGRVSDLRAICASEKVVSQCGIGHTRWATHGGVNDCNAHPHQVGKVTLVHNGI
                     IENYRELIADYDLADTLKSETDSEVVAALLNRFYEGNPEEAIKKTVSKLKGTFALVIL
                     FEDQKDVIYSTRNVSPIVATICKEGAMLASDLTALCRFTNRYFVVPEYHILKLSADKL
                     TLTDFDGNEVLPKYLTVDWELNSAGKNGYPFYMEKEIMEQPEAIENTIRNRIVGGMPD
                     FTADGVPDTLFTECEHICIVACGTAMHAGLVAQALVKSILHMHIEVQMASEFMYSDPV
                     IDEKTLVIAVSQSGETIDTLEAMKYAKNRGAKCLAIINVKGSSIARESDYVLYTNAGP
                     EIAVASTKAYTTQLAVFYLIVARMAHSRGVFDDAQTQSFVRELQRTPEVMKKVLERRR
                     DIHVVAKKVLGAKDLFMIGRGLDYSILLEGSLKLKEVSYIHSEAYASGELKHGPIALI
                     TQDTPVVATVTQEKLMSKELSNIKEVKSRGADVVVFIKESIVGDLAKEYEIFTLPDMQ
                     DEFMVLPASVALQLLAYYVSSDKGFDVDKPRNLAKSVTVE"
BASE COUNT          530 a          705 c          479 g          641 t
ORIGIN      
        1 attggatcgc tcattgacat agctttgttt cctccttaga gttcccatac acttcacact
       61 tagaatcttc gtcagttccc gaatctaaga gatcaaaaaa ttcattttcc tgcgaaacgc
      121 ccatttcaag taacagaatt gtacctgcct tcataatgca acgcaggcac aattcgtccc
      181 ttgccacaac ctcacgtttc gcaacaatcc ccttactcca cagttacgga ttttgccagg
      241 tttcttggct tatctacatc gaatccctta tcggaagaca catagtacgc aagcagctgc
      301 aatgccaccg atgccggcag caccatgaac tcatcctgca tatccggcag cgtgaaaatc
      361 tcgtactcct tcgctaaatc tcccacaatg ctttccttga taaaaacgac aacgtccgct
      421 cctctggact ttacctcctt aatgttggaa agttccttgg acatcagttt ttcctgcgtc
      481 accgttgcca ccaccggcgt atcctgcgtg atcagcgcga tcggaccgtg ctttaattct
      541 ccggatgcgt acgcctccga gtggatgtag gatacctcct ttaacttcag cgatccctcc
      601 agaagaatcg aataatccag tcctcttcca atcatgaaca aatccttagc gccgagcacc
      661 tttttggcaa ccacatggat atccctgcgg cgctccagca ccttcttcat gacctccggt
      721 gtccgctgca gctcccgcac aaagctctgg gtctgcgcgt cgtcgaagac acctctgctg
      781 tgcgccatac gcgccacgat cagataaaat accgcaagct gtgtcgtgta cgccttggta
      841 cttgccaccg cgatctccgg tcccgcattg gtgtagagca catagtcgct ctctcttgca
      901 atggaggaac ccttgacgtt gatgatcgca agacactttg ccccgcggtt cttcgcatac
      961 ttcatcgcct ctaacgtatc gatcgtctca ccggactgcg acacggcaat cacaagcgtc
     1021 ttctcgtcga tgaccggatc cgaatacatg aactcggaag ccatctgcac ctcaatgtgc
     1081 atgtgaagaa tggacttgac gagcgcctgt gccaccagtc ccgcgtgcat tgcggttccg
     1141 cacgctacga tgcagatatg ctcgcactct gtaaaaagag tgtccggcac gccgtctgct
     1201 gtaaaatccg gcatacctcc gacgatacgg ttcctgatgg tattctcgat ggcttccggc
     1261 tgctccataa tctctttttc catatagaac ggataaccgt tcttcccggc gctgtttaat
     1321 tcccagtcca cggtcagata ctttggcagc acctcattgc cgtcaaaatc cgtcagtgtc
     1381 agtttgtcag cggacagctt taagatatga tactccggca cgacaaaata acggttcgta
     1441 aaacggcaga gcgccgtcag atcactggca agcatcgcgc cttccttaca gatcgtcgcc
     1501 acgatcgggc tgacattgcg cgttgaatag atcacatcct tctgatcttc aaataaaatg
     1561 acaagcgcaa atgttccctt cagcttcgag acggtcttct tgatcgcttc ctccggattc
     1621 ccttcataga accggttcaa aagcgctgcc accacctcgc tgtccgtctc ggacttcagt
     1681 gtatctgcca ggtcgtaatc cgcaatcagt tccctgtaat tctcaatgat gccgttgtgt
     1741 acgagcgtga cttttcccac ttgatgcggg tgtgcattgc agtcgtttac accgccatgc
     1801 gtcgcccatc tcgtatgacc gattccgcac tgtgacacca ctttttccga cgcgcagatc
     1861 gcccgcagat cactgactct tccggcacat ttgcggactt ctgttttccc gctctcctcg
     1921 tcgcgcaggg cgataccggc gctgtcgtac cctctgtact ccaacagctc cagcgcatcc
     1981 aaaaggactt ctcttacgtc ctcacttcct gtataaccta taattccaca catatgtttt
     2041 ccccatttct attacaatct gtatataaca atatttttat tcaggtctct tctgtttcta
     2101 ttccatcaaa gtctgttcca tcaaacatcg tatttgtttt gcagtcaccc gcatatttgc
     2161 cgatgtgtct cgcactggaa cggtacggaa aggcatccgc cgaattttcg atcacctttc
     2221 tcctcgttaa cctgccaggg caggtgcatg gcgcttaggg aatctctgtg gattacggta
     2281 cagatcagat tgcgtatctg aacatataac gttcccctta tgcagttatc aaggttcaac
     2341 gtgtttacta tacta
//
LOCUS       DBMDQJ010000395         2353 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15854_length_2353_cov_1.619893, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000395 DBMDQJ010000000
VERSION     DBMDQJ010000395.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15854_length_2353_cov_1.619893"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZO9R_08310"
     CDS             complement(<1..207)
                     /locus_tag="ACZO9R_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913103.1"
                     /translation="MNGKNIKKHLNNKLRDWILNIDDESIKKIIRDNVIVTGGAIVSL
                     LTGEKLHDYDVYFRTKEACLAVATY"
     gene            complement(210..1106)
                     /locus_tag="ACZO9R_08315"
     CDS             complement(210..1106)
                     /locus_tag="ACZO9R_08315"
                     /inference="COORDINATES: protein motif:HMM:NF018751.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HIR0913104.1"
                     /translation="MQEINLLVEQKDGSIETNFEEIKVALAAGLEEYKGMVFTAESQP
                     EAKRTVASLRKLKKAMNDRRIEIKKTFMAPYTNFEAQVKELDKLIDEPIDFISGQIEE
                     FERRRVEAKKAMICEIYTGIMAEHGTVMEYLPLDRIYDSRWENSTTTQKAITEAITAH
                     VEHVEKDLDTIRAMESEFEDKGLAKYKTTLELSDAITIMNQYQKQKEEILRRQAEEEQ
                     RKAEEEARRAAEEEQRKAALVHEESVVPEVVPDVALEEEKAVRSATVPEGTVRYEVVA
                     DPFQIAQLEAAMREYGIKFRRV"
     gene            complement(1093..1242)
                     /locus_tag="ACZO9R_08320"
     CDS             complement(1093..1242)
                     /locus_tag="ACZO9R_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913105.1"
                     /translation="MIEIAPDAPDWDEYEAEQARAQRHRKKLAAIYDRDERFREEKEI
                     EDAGN"
     gene            complement(1235..1456)
                     /locus_tag="ACZO9R_08325"
     CDS             complement(1235..1456)
                     /locus_tag="ACZO9R_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913106.1"
                     /translation="MKARLCNKDMCNLVDVLRELPETLEGVGVAGIATITVTDDGSIS
                     GVLAVSPETAVRLKISDNGDKGEWEYIDD"
     gene            complement(1437..1631)
                     /locus_tag="ACZO9R_08330"
     CDS             complement(1437..1631)
                     /locus_tag="ACZO9R_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913107.1"
                     /translation="MIFRECKRCGHPMDPGEGQNGMCDDCVTGETERREREEKMEWMI
                     RATDWTQMEVEDFLNESKVM"
     gene            complement(1729..1995)
                     /locus_tag="ACZO9R_08335"
     CDS             complement(1729..1995)
                     /locus_tag="ACZO9R_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR0913108.1"
                     /translation="MKRIGKIITAVGVGIMFFGGMCDADGIYYYYLIAVIALGALVSL
                     AGLAIMSVELRRAERRKACFYFIRRRDRLDADVEFIDLDKKIAP"
     gene            complement(2036..2185)
                     /locus_tag="ACZO9R_08340"
     CDS             complement(2036..2185)
                     /locus_tag="ACZO9R_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913109.1"
                     /translation="MAKRKKKEITCSVTITEGASERLTKALVKIHYQRKRDEEMRGIT
                     KDETA"
BASE COUNT          495 a          731 c          404 g          723 t
ORIGIN      
        1 gtatgtcgct acagccaagc acgcttcttt tgttctaaaa taaacatcat aatcgtgaag
       61 tttttcacca gtcaaaagtg aaacaattgc accgccagta acaatcacat tatcccttat
      121 aatctttttg atgctttcgt catcaatatt caaaatccaa tcgcgcaact tgttattcaa
      181 gtgctttttg atgtttttac cattcatctc tataccctcc gaaatttaat accgtactca
      241 cgcatagcag cttcaagctg tgcgatctgg aacggatccg caaccacttc ataccgtacg
      301 gttccctcag gcaccgttgc agaccgcaca gctttttctt cctccagagc aacatccggc
      361 acaacttccg gtacaaccga ttcttcatgc accaaagcag cttttctctg ctcttcctct
      421 gccgccctgc gtgcctcctc ttctgccttt ctctgctctt cctctgcctg tctccgcaga
      481 atttcttcct tctgcttctg gtactgattc atgattgtaa tggcatctga cagttccagc
      541 gttgtcttat acttcgccag acctttatcc tcaaactccg attccatcgc ccggatagtg
      601 tccagatctt tttctacgtg ctccacatgt gctgtgatgg cttctgtgat ggctttctgc
      661 gtggtcgtgg aattctccca tctgctgtca tagatgcgat ccagcggcag atactccatc
      721 acggttccat gctccgccat aatcccggta tagatttcac agatcatcgc tttctttgct
      781 tccacgcgcc tacgctcaaa ctcctcgatc tgcccgctga taaagtcgat cggttcatcg
      841 atcagcttgt ccagttcctt gacctgcgct tcaaaattgg tatacggcgc cataaaagtt
      901 ttcttgatct cgattcgcct gtcgttcatg gcctttttca gcttacgcag gcttgccacc
      961 gtcctttttg cttccggctg ggattctgcg gtaaacacca tccccttgta ctcctccagt
     1021 cctgccgcaa gggctacctt gatctcttca aagtttgtct cgatacttcc gtctttctgc
     1081 tctactaata agttaatttc ctgcatcttc tatctccttt tcttctctaa accgctcatc
     1141 gcggtcgtat attgctgcca gtttctttct atgccgctgt gcccgtgcct gctccgcttc
     1201 gtattcgtcc cagtccggcg catccggcgc gatctcaatc atcgatatac tcccactctc
     1261 ctttgtcgcc attgtcactg atcttaagtc tcactgctgt ctctggcgaa acagccagca
     1321 ccccgctaat gctcccgtca tccgtaacgg taatagtggc aattcccgca acgccgaccc
     1381 cttccagtgt ttccggcaat tcccgtaaca catccacgag attgcacatg tccttgttac
     1441 ataaccttgc tttcattcaa aaaatcctcc acttccatct gcgtccaatc cgttgcccgg
     1501 atcatccact ccatcttctc ttcgcgctcc cgccgctctg tttcaccggt aacgcagtca
     1561 tcacacatac cgttctgacc ctctcccggg tccattggat gaccgcagcg cttacattct
     1621 ctgaaaatca taaaatcaca ctttcaaaaa ctgctctttc gtgttacaat aaacgcagaa
     1681 atacttatgt attcctacgg taaatagcac ctgcgttcgc caaaacagtc agggtgctat
     1741 ttttttgtcc aaatcgataa actccacatc cgcatccagc ctgtcccgtc tgcggataaa
     1801 gtaaaaacat gctttccgcc gctcggctct gcgcagctcc accgacatga tcgccaagcc
     1861 cgccaatgat accaacgcgc ctaacgcaat cacggcaatg aggtagtaat aataaatgcc
     1921 gtctgcatca cacattccac cgaaaaacat tatgccgact ccgaccgccg taatgatctt
     1981 gcctatcctt ttcaacgttc tcactccttt cttatggctt gtccgccgct accgcctaag
     2041 cggtttcatc cttcgttatc ccgcgcatct cttcatcacg cttccgctga taatgaatct
     2101 taaccaatgc cttggtaagc cgttccgatg ctccctcggt tatcgttacg ctgcaggtaa
     2161 tctccttttt ctttctcttt gccatacatt tccacctccg ttaaatcata tgacagcctg
     2221 cttgtatcct tttcctgctt tgtgcatcat ctcctccgtt gtcaaactat tcttgtacgc
     2281 tattcttcat gtgtggtaca atctccttac aggacgttgc cgcgtccgag tattatgaaa
     2341 ggagatatcg aca
//
LOCUS       DBMDQJ010000396         2351 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15872_length_2351_cov_1.436000, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000396 DBMDQJ010000000
VERSION     DBMDQJ010000396.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15872_length_2351_cov_1.436000"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1531
                     /locus_tag="ACZO9R_08345"
     CDS             <1..1531
                     /locus_tag="ACZO9R_08345"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - obsolete glycogen debranching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0019156 - isoamylase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen debranching protein"
                     /protein_id="HIR0913110.1"
                     /translation="LRQKIPYLKDLGVNAIELLPIFEFDEMESARVVDGVQLYNYWGY
                     NTVSFFAPNTSYSSVVEHNHEGDELKLLISELKANGMEVILDVVFNHTAEGNENGPCF
                     SFKGIDNNIYYMLTPDGHYYNFSGCGNAMNCNHPIMRKFIIDCLRYWVMEYRVDGFRF
                     DLASILTRDQNGAPMPDPPILQGIACDPILGHVKLIAEAWDAAGLYQVGSFPAFRRWS
                     EWNGRYRDDMRRFLKGDGSMAGTAINRIIGSTDLYDPVHRGESASVNFLTCHDGFTLY
                     DLYSYNTKHNEKNGWNNTDGDNNGNSWNCGAEGETDDPQIEELRRRMVKNAFATLLCS
                     RGPAMFYGGDEFCNTQFGNNNAYCQDNIISWLDWTRLEKYREIHDFVRYMIGFRKRYA
                     ILRKKTKPVACNLPEISIHNGYPWNGGTDSNSRLIGIMYAGRDEHDTRDDIVFYCMNA
                     YWETLVMQLPELPNGLQWKVCVNTSVEYEDGRDMESYTEFYYKKTLKVPPRTAIVLVA
                     E"
     gene            complement(1587..2315)
                     /gene="ymfI"
                     /locus_tag="ACZO9R_08350"
     CDS             complement(1587..2315)
                     /gene="ymfI"
                     /locus_tag="ACZO9R_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P 5-aminopentanone reductase"
                     /protein_id="HIR0913111.1"
                     /translation="MYKTVLITGASRGIGRAIACAYAREGCRLVINCSRSEKELLALA
                     DELRETCHVDVLTSIGDVSDYAYMEQLFSQSEARFGGVDILVNNAGISHIGLLEDMTI
                     DEWNRIIGVNLTSVFSASKLALPHMIHQKSGKILNISSIWGNVGASCEVAYSACKGGI
                     NSFTRALAKELAPSNIQVNAIACGVIDTEMNACFSSEERTQLAEEIPAGRFGAPEEVA
                     SLAVQITTGNDYLTGHITLDGGYL"
BASE COUNT          540 a          559 c          725 g          527 t
ORIGIN      
        1 actgcgccag aagattccgt atctgaagga tcttggcgtc aacgcgatcg agctgctccc
       61 gatttttgag ttcgatgaga tggagagcgc ccgcgtggtg gacggtgtgc agctgtacaa
      121 ttactggggg tacaatacag tgagcttttt tgcgccgaac accagctatt cgtccgtggt
      181 agagcacaat cacgagggag acgagttaaa gctgctgatc agtgaactca aggcaaacgg
      241 catggaagtc attctggatg tcgtgtttaa ccatactgcc gagggaaatg agaatggacc
      301 gtgtttttca tttaagggca ttgataataa tatttattat atgctgacgc cggatggaca
      361 ttactacaat ttcagcggat gtggcaatgc catgaactgc aaccacccga tcatgcgcaa
      421 gtttatcatt gactgcctgc gctactgggt gatggagtac cgcgtggacg gtttccggtt
      481 cgatctggct tcgattctga cacgtgacca gaacggggcg ccgatgccgg atccgccgat
      541 tttacagggg atcgcctgcg acccgatctt aggacatgtg aagctgatcg ccgaggcgtg
      601 ggatgcggca ggactttacc aggtgggaag ctttccggca tttcgcagat ggtcagagtg
      661 gaacggcaga tacagagatg atatgcgccg ctttttaaag ggcgacggga gcatggcggg
      721 aaccgcgatc aaccgtatca tcggttcgac ggatctgtac gatccggtgc acagaggtga
      781 gagcgcatcg gtgaatttcc tgacctgtca tgacggtttt acgctctatg atctgtactc
      841 gtacaatacg aagcacaatg agaaaaacgg ctggaacaat acggacggcg acaacaacgg
      901 aaacagctgg aactgcggtg ccgagggaga gacggacgat ccgcagatcg aggaactgcg
      961 ccggcgcatg gtgaaaaatg cgtttgcgac gctgctctgc agccgcggac cggcgatgtt
     1021 ctacggcggc gacgagttct gcaacacgca gtttggcaac aacaatgcct actgtcagga
     1081 caatatcata tcctggctgg actggacgag actggaaaaa taccgggaga ttcatgattt
     1141 tgtgcgctat atgatcggtt tccggaagcg ctatgccatt ttgcgcaaga agacgaagcc
     1201 ggtggcatgt aatctgccgg agatcagtat tcacaacggt tacccgtgga acggcggtac
     1261 ggattccaac agccgtctca tcggcattat gtatgcggga agagatgagc acgacacgcg
     1321 ggacgatatt gtgttctatt gtatgaatgc ttactgggag acgttagtga tgcagcttcc
     1381 ggaactgccg aacgggcttc agtggaaagt gtgtgtcaac acgtccgtgg agtacgagga
     1441 cggcagggat atggagtcct acacagagtt ttattataag aagacattga aggttccgcc
     1501 gcgcacggcg atcgtactgg tggcagagta agcgggtgaa gcgggcagaa acccttgggg
     1561 aatgcccgct gcctgcgttg cggctcctag aggtagccgc cgtcgagggt gatgtgcccg
     1621 gtcaggtagt cgtttccggt ggtaatctgc acggcaagcg atgccacttc ctccggcgcg
     1681 ccgaagcgcc cggcggggat ttcctcggcg agctgggtgc gctcttccga ggaaaagcag
     1741 gcattcatct cggtgtcgat cacgccgcag gcgattgcgt tgacctggat gttactcgga
     1801 gcgagttcct tggcgagggc gcgggtgaag gaattgatgc cgcccttgca ggcggagtat
     1861 gcgacctcgc aggaggcacc gacatttccc cagatcgagg agatgtttaa gattttcccg
     1921 gatttctggt ggatcatgtg cggcagggca agcttgctcg cggaaaagac ggaggtcagg
     1981 ttgacgccga tgatgcggtt ccactcatcg atggtcatat cttccagcag accgatatgt
     2041 gagatgccgg cattgtttac gagaatgtca acgccgccga agcgggcttc gctttgggaa
     2101 aaaagctgtt ccatataggc atagtctgac acgtcgccga tgctggtgag tacgtccaca
     2161 tggcaggttt cccttaattc gtctgccagc gccagcagct ccttctcgga gcgggaacag
     2221 ttgatgacca gacggcagcc ctctctggcg taggcgcagg cgatcgcccg cccaatgccg
     2281 cgtgaggcgc cggtaatgag tacggtttta tacatgatga aagacctttc ttctatttgt
     2341 attgtgaata c
//
LOCUS       DBMDQJ010000397         2340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15964_length_2340_cov_1.493971, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000397 DBMDQJ010000000
VERSION     DBMDQJ010000397.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15964_length_2340_cov_1.493971"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="ACZO9R_08355"
     CDS             <1..188
                     /locus_tag="ACZO9R_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009269049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR0913112.1"
                     /translation="CVCDLAEALNMTQSAISHQLRILKQNKLVKSRREGKSVFYSLAD
                     GHVRTIIAQGREHIEEK"
     gene            220..438
                     /locus_tag="ACZO9R_08360"
     CDS             220..438
                     /locus_tag="ACZO9R_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HIR0913113.1"
                     /translation="MKKKFKLQDLDCANCAAKMEEAIKKIPGVNDANVSFMTQKMTVD
                     AEDDKFDDIMKEVVSVCAKVEPDCKILM"
     gene            579..>2340
                     /locus_tag="ACZO9R_08365"
     CDS             579..>2340
                     /locus_tag="ACZO9R_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR0913114.1"
                     /translation="MTKKQKRMLVRIIISFVLFAALMVAEHTGALEGINTWILFVIYL
                     VPYLVIGYDIVYKAVRNISHGQVFDENFLMMVATFGAFGVKEYSEAVAVMLFYQVGEL
                     FQGYAVGKSRQSISAMMDICPEYANIEVDGKLTQVDPDDVEVGSVIVVKPGERIPLDG
                     IVLEGESFIDTAALTGESVPRKAAAGDEIISGCVNGSGTLKVQTTKEFDDSTVAKILE
                     LVENASSKKARVENFITRFAKYYTPVVTIGAVVLAILPPLILGGGWSDWIQRACIFLV
                     ISCPCALVISVPLGFFGGIGAASKVGVLVKGSNYLEAVAEMTTIVFDKTGTLTKGEFK
                     VSEILPAEGSKEELLEIAALGEGYPNHPIAASIREAYGKELDMNRITDAEEVAGHGIS
                     VRIDGRNVLIGNEKLMKEKAIFYEPCKNIGTVVYVAGEGKFLGSIVIADTVKDGAKEA
                     IASMKKVGVKKCVMLTGDRKEAAEGIAAELGIDEVHAELLPQDKVSQVERLLKEENGK
                     EKLAFVGDGINDAPVLTRADIGIAMGSMGSDAAIEAADVVLMDDDIRKIASIVRISRK
                     TLGIVKQNIVFALAVKAIVLV"
BASE COUNT          654 a          465 c          713 g          508 t
ORIGIN      
        1 tgtgcgtgtg cgatcttgcg gaggcactga atatgacaca gtctgcaata tcacatcagc
       61 ttaggattct gaagcagaac aaactggtca agagcagaag ggaaggaaag tcggtttttt
      121 attctctggc agacgggcat gtgcgtacga tcatcgcaca gggccgcgaa catattgaag
      181 aaaaataaag aagaaatatt ataaaaggag tgttttatca tgaagaaaaa atttaaatta
      241 caggatctgg attgtgcaaa ctgcgcggca aaaatggaag aggcgatcaa gaagattccg
      301 ggcgtcaatg atgcaaatgt cagcttcatg acacagaaga tgacggtaga tgcagaggat
      361 gacaagttcg acgacatcat gaaagaagtc gtttctgtct gcgcaaaggt agagccggac
      421 tgcaagatct taatgtaata acatgggcgt gtgcccggca gaaaacgaaa gacaattcca
      481 tgaaagcctg tgtgccggag ggtgcgcagg cttagaaaaa gagatacata tgaacgaatg
      541 tttatgcgtt tatgaaaatg aaaagcgagg tgtgtggtat gacgaagaaa cagaaacgga
      601 tgctggttcg gatcatcata tcctttgtcc tgttcgcagc cctgatggtg gcggagcata
      661 cgggggcatt ggaaggaatc aatacatgga tattatttgt catctatctg gtgccttacc
      721 tggtcatcgg atatgacatt gtatataaag cggtgagaaa tatcagccac ggacaggtgt
      781 ttgatgaaaa cttcctgatg atggtggcga cgtttggcgc atttggcgtg aaggaatact
      841 cggaagcggt tgccgttatg ctgttttacc aggttggtga gctgttccag ggctatgcgg
      901 tcggcaagtc cagacagtcg atctcggcga tgatggacat ctgtccggag tatgccaata
      961 tcgaggtgga cggaaaactg acgcaggtcg atccggatga tgtggaagtc ggcagcgtga
     1021 tcgtggtaaa accgggcgag cgtattccgc tggacggcat tgtgctggaa ggggagtcct
     1081 tcattgatac agcggcgctg accggagaat cggttcctcg caaggcggca gcaggagatg
     1141 agatcatcag cggctgtgtc aacggaagcg gtacgctgaa agtgcaaaca accaaggagt
     1201 tcgatgactc caccgttgca aagattctgg aacttgtgga aaacgcgagc agcaaaaagg
     1261 cacgcgtgga aaactttatc acgcgttttg caaaatatta tacaccggtt gtcacgatcg
     1321 gtgcggtggt tctggcaatt ctgccgccgc tcattctcgg cggaggatgg agcgactgga
     1381 ttcagagagc ctgcattttc cttgttatct cctgcccgtg cgcgcttgtg atctctgtgc
     1441 cgctgggatt ctttggcgga atcggcgcgg cgtcgaaggt gggcgtactt gtaaagggaa
     1501 gcaattatct cgaagctgta gctgaaatga caacgattgt ctttgacaag acgggtacgc
     1561 tgacgaaggg cgaattcaag gtttctgaga ttctgccggc tgagggaagt aaagaagaac
     1621 ttttagagat tgcggcgctc ggcgagggat acccgaatca cccgattgcg gcatccatcc
     1681 gggaagccta tggaaaagaa cttgacatga accgtatcac agacgccgag gaagtggcgg
     1741 ggcacggaat cagtgtcaga atcgacggaa gaaatgtgct catcggcaat gagaagctga
     1801 tgaaggaaaa agcaattttc tatgagccgt gcaagaacat cggaaccgtt gtctatgtgg
     1861 caggagaagg aaaattcctc ggaagcattg tgattgccga tacggtaaag gacggagcaa
     1921 aagaagcgat cgcctccatg aagaaagtgg gtgtgaagaa atgtgtcatg ctgaccggtg
     1981 acagaaaaga agcagcagag ggaatcgctg cggaactcgg catcgacgag gtgcatgcag
     2041 aactgctccc gcaggacaaa gtgtcgcagg tggagcgcct gttaaaagaa gagaacggga
     2101 aagagaagct ggcatttgtc ggcgacggta tcaacgatgc gccagtgctc accagagcgg
     2161 atatcggcat tgcaatggga agcatgggct ctgacgcggc gattgaggcg gcagatgtgg
     2221 tcctgatgga cgacgacatc cgaaagattg cgtcgatcgt gcggatctcc agaaagacac
     2281 tcggcattgt aaagcagaac atcgtctttg cactggcggt caaagcgatc gtgctggttc
//
LOCUS       DBMDQJ010000398         2340 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_15967_length_2340_cov_1.370255, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000398 DBMDQJ010000000
VERSION     DBMDQJ010000398.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15967_length_2340_cov_1.370255"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(124..1725)
                     /locus_tag="ACZO9R_08370"
     CDS             complement(124..1725)
                     /locus_tag="ACZO9R_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0913115.1"
                     /translation="MLKVFLAEDEIVMREGIKNNIDWQGEGFEFVGDAGDGELAYPII
                     QKTRPDILITDIKMPFMDGLELSRLVKHELPETKIILLSGYDEFQYAKEAIDIGITDY
                     LVKPVTGAQLIGTLKKIGQKIEEERGKQKQQTPEEVRSAGRQRLFRHMVSGKHTYASV
                     FQEAKELGLELIAEQYNIMMLQLFSEEKEDVLPEKIKAFEQELEKYMEQSGNLIVAKQ
                     GRAEYDFVLKGTGQQSLAETTEQVKSLLAEYFRRETAVEYVAALGTPVERFSELQQCY
                     RQTNLCFSRRYSVERNQILEEETPGETAAEVLEDVKLGELNISSLSQRQAEAFLYTGT
                     REEVSGFVEDYFAQLGDGNVKSIFLRQYVVMDLYVTAVSVLEKSGYKSRDLVERCGDA
                     KEMAAYLTTVEQTREYIKKLFSAVLDLRSEAVDGKYDGIMQKAKTYINDHFEKDGISL
                     NMVAAEVNLSPSHFSTVFGQEMGETFVEYLTRIRMEKAKELLRTTGKKVTEIAFEVGY
                     RDAHYFSNLFKKTQGCTPGEYRSRG"
     gene            1810..2088
                     /locus_tag="ACZO9R_08375"
     CDS             1810..2088
                     /locus_tag="ACZO9R_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913116.1"
                     /translation="MAVRDAYHRILLDAVDTLVQKCPPAYAGLPALRERIYLGIQLLE
                     SFAHTQLATSLCSQFDMESMQTLCLRIAVELLTPPHQTGSDTAVSCSH"
     gene            complement(2080..>2340)
                     /locus_tag="ACZO9R_08380"
     CDS             complement(2080..>2340)
                     /locus_tag="ACZO9R_08380"
                     /inference="COORDINATES: protein motif:HMM:NF023367.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HIR0913117.1"
                     /translation="KQLIAMRRQYPQTRSGKVVWDVREDSRLLHYYKKKEGQKTLEVC
                     INAGEEPVRTGERGLPEPEDCLFANGLEHGSLRPQGVFIRLV"
BASE COUNT          479 a          772 c          487 g          602 t
ORIGIN      
        1 aatcttgttc gtcataatca gaagatacgc cagaagtatg gtcagcggca ccacacagat
       61 cagcagcagc atatagagcc tgctctttag tgaaaaccct ctcttcaata tccatctccc
      121 cttttaccct ctgctgcggt actcacccgg tgtgcagccc tgcgtcttct tgaacaggtt
      181 gctgaaataa tgcgcatccc ggtagcccac ctcaaaggca atctccgtta ccttctttcc
      241 cgtcgtcctg agcagctcct tcgctttttc catgcggatt cttgtaagat attctacgaa
      301 cgtctctccc atttcctgac cgaataccgt gctgaaatgg ctcggactca gatttacctc
      361 cgctgcgacc atattcaggg aaatcccgtc cttctcaaaa tgatcgttga tgtaggtctt
      421 tgccttctgc atgatgccgt cgtattttcc gtccaccgcc tcactgcgca aatccagcac
      481 cgccgaaaaa agcttcttaa tatattccct ggtctgctca accgtggtca ggtaggctgc
      541 catctccttc gcatcgccgc accgctccac gagatccctg ctcttgtaac cgctcttctc
      601 cagaacggaa accgccgtca catacagatc catcaccacg tactggcgca ggaagataga
      661 ctttacattg ccgtccccga gctgtgcaaa gtaatcctcc acgaagccgg acacctcctc
      721 cctagtgccc gtgtacagaa aagcctctgc ctgccgctga ctcaggctgg agatattcag
      781 ctccccgagc tttacatcct ccagaacctc cgccgcagtc tcccccggcg tctcctcctc
      841 aaggatctgg ttgcgctcta ccgaatagcg tctggaaaag cacaggttgg tctgccggta
      901 gcactgctgc agctcgctga aacgctccac cggtgtcccg agcgccgcga catactccac
      961 cgccgtctct ctccggaaat actctgccag caggcttttt acctgctctg tcgtctccgc
     1021 gagagactgc tgccctgtgc ccttcagcac aaaatcgtac tccgcccggc cctgctttgc
     1081 gacaatcaga ttgccggact gctccatgta cttctccagc tcctgctcga aggcttttat
     1141 cttctccggc aggacatcct ccttctcctc ggagaacagc tggagcatca tgatattgta
     1201 ctgctccgca atcagctcaa gtcccagttc cttcgcctcc tggaacaccg acgcataggt
     1261 atgctttccg gagaccatgt gccggaacag ccgctgcctg ccggcagatc tcacctcctc
     1321 cggcgtctgc tgcttctgct tcccgcgctc ctcctcgatc ttctgaccga tcttcttgag
     1381 tgtcccgatc agctgtgcac ccgtgaccgg cttcaccaga taatccgtga ttccgatgtc
     1441 gattgcctct tttgcatact ggaactcgtc atacccgctc aggagtatga tcttggtctc
     1501 cggcagctcg tgcttcacga gacggctcag ctccagtcca tccatgaacg gcatcttaat
     1561 gtccgtgatc agaatatccg gtctcgtctt ctggatgatc ggataggcga gctccccgtc
     1621 gcccgcgtca ccgacaaatt caaagccttc accctgccag tcgatgttgt tctttatgcc
     1681 ctcccgcata acgatctcat cttccgctaa aaaaaccttt aacatatgaa attcccctca
     1741 atacattttc ctgtaaaaac cattgtaaat tcattttatc acacctgcga acaaaaaaca
     1801 cagaagattt tggctgtccg ggatgcctat caccggattc tgctcgatgc agtcgatacc
     1861 ctcgtgcaga aatgtccccc cgcatacgca ggacttccgg cgctcaggga acgcatctat
     1921 ttaggtatcc agcttctgga aagctttgcg cacacccagc tggcgacttc cctctgcagt
     1981 cagtttgata tggaatccat gcagacactc tgcctccgga ttgcagtgga gctgctgaca
     2041 ccgccgcatc agacgggatc cgatacggca gtgtcatgtt cacactaatc ttatgaaaac
     2101 gccctgcggt cttaagcttc cgtgctccaa tccgttcgcg aacaggcagt cctccggctc
     2161 cggcagtccc cgctcccctg tccgcaccgg ttcctccccg gcgttaatgc agacctccag
     2221 cgttttttgt ccttcttttt tcttataata atgtaacaat ctggaatcct ctctgacatc
     2281 ccataccact tttccgctcc ttgtctgcgg atactgcctg cgcatcgcaa tcagctgctt
//
LOCUS       DBMDQJ010000399         2323 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16106_length_2323_cov_1.735374, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000399 DBMDQJ010000000
VERSION     DBMDQJ010000399.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16106_length_2323_cov_1.735374"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(113..547)
                     /locus_tag="ACZO9R_08385"
     CDS             complement(113..547)
                     /locus_tag="ACZO9R_08385"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR0913118.1"
                     /translation="MPQIRIKYFTDAIERLTYIDGKSDWIDLRASEEVTLKAGEFCLI
                     PLGVAMELPEGYEAHVVPRSSTFKNYGIIQTNSCGIIDGTYCGDDDMWRMPVYAVRDT
                     VIHVNDRICQFRIFENQPKITFDEVESLGNKNRGGFGSTGKQ"
     gene            complement(643..1656)
                     /locus_tag="ACZO9R_08390"
     CDS             complement(643..1656)
                     /locus_tag="ACZO9R_08390"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR0913119.1"
                     /translation="MAKVAVIGAGSWGTALAKVLHTNGSQVTVWSIVEAEIAMLRERH
                     EHVDKLPGVKLPEDMEFTTDLKAAVEDKEYLILAVPSVFTRSTAKSMAPFVKAGQIVV
                     CVAKGIEENTLMTLSDIVEEEVPAAEVAVMCGPSHAEEVGVGLPTTVVAGAKKRKVAE
                     GVQDIFMNEVFRVYTSPDVLGMELGGSLKNVIALAAGMADGLGYGDNTKAALITRGIT
                     EMSRLAIKMGAKAETLSGLTGIGDLIVTCESRHSRNRKAGMLIGQGYTMKQAMDEVKM
                     VVEGVYSAKAAIALAKKYGVDMPIIEEVNRVLFEDKPAKEAVRELMMRDRRAEHSALE
                     WEA"
     gene            complement(1662..2321)
                     /gene="plsY"
                     /locus_tag="ACZO9R_08395"
     CDS             complement(1662..2321)
                     /gene="plsY"
                     /locus_tag="ACZO9R_08395"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR0913120.1"
                     /translation="MIGARVIALGIGYLFGLFQTGYLYGKSQGIDIRSEGSGNAGTTN
                     SLRVLGIKAGLITFAGDLCKAILAVLLVKVLFRNAYPDAVKILELYAGFGAVLGHNFP
                     FYLKFKGGKGIACTSGMILAVCPMAAPVCLILFIGSIASTRYVSLGSILVVMSYLVQV
                     LIFGHMGYLHIDAAYLPEFYVVSACFTVMALWRHRSNIGRLLHGTENKFTFKKNKQEG
                     K"
BASE COUNT          509 a          740 c          481 g          593 t
ORIGIN      
        1 acaaagaatt tttgcttttc ttgtagaatg tcacagtgtt ttggttgttt tttctattct
       61 ttcgtcaaac cgacaggttt tccatcaaaa agtaagcact ttgacgaacg cattactgtt
      121 tgccggtgct tccgaatccg ccgcggtttt tgttgccgag gctctccact tcgtcaaacg
      181 tgatcttcgg ctgattttca aaaatgcgga actggcagat gcggtcattg acgtgaatca
      241 ccgtgtcgcg caccgcatag accggcatcc gccacatatc gtcatccccg cagtaggtgc
      301 catcgataat gccgcagctg ttcgtctgga tgattccgta atttttaaaa gtggaacttc
      361 tcggcaccac atgcgcctca tagccctccg gaagctccat ggcaacgccg agcggaatca
      421 gacagaactc tcccgctttc agtgtcactt cctcagacgc ccgaagatcg atccagtccg
      481 actttccgtc aatataggtc agacgttcaa tcgcatccgt aaaatattta atcctgatct
      541 gtggcatgta tgctcctcct gttatgtctg tctttttctt tctatgcgtc ccactccagg
      601 gcactatgca gagcatagtg ccctaactta atgccgatcc ctttacgctt cccactccag
      661 agcactatgc tctgcacgcc ggtcgcgcat catcagttcc cgcaccgctt cttttgcagg
      721 cttatcctca aaaagcacac ggttgacttc ctcgatgatc ggcatatcga ccccgtattt
      781 ttttgcaagc gcgatcgccg ccttggcgga atacacgccc tcgaccacca tcttgacctc
      841 atccatcgcc tgcttcatag tatagccctg cccgatcaac attccggctt tgcggtttct
      901 gctgtgtctg ctctcacatg tcacgatcag atctccgatt ccggtcagac cgctcagtgt
      961 ctccgccttt gcccccatct tgatggcaag acgcgacatt tccgtaattc cgcgcgtgat
     1021 taaagccgct ttggtgttgt ccccgtagcc gagtccgtcc gccataccgg ctgccagtgc
     1081 aatgacgttc ttcagggaac cgcccagttc catgccgagc acatccgggc ttgtgtacac
     1141 gcggaacacc tcattcataa aaatatcctg cacgccctct gccaccttgc gcttcttcgc
     1201 tccggcaaca acggtcgtcg gcaatccgac gccaacctcc tcggcgtggc tcggtccgca
     1261 catcacggca acctccgccg caggtacttc ctcttctaca atatctgaga gcgtcatcag
     1321 cgtattttcc tcaatgccct ttgccacaca gacaacgatc tgtcccgcct tcacaaaagg
     1381 cgccatgctt tttgccgtgc ttctggtgaa gacagacggt accgccagaa tcagatattc
     1441 cttatcttcc actgccgcct taagatccgt ggtaaattcc atatcctccg gaagcttcac
     1501 tcccggaagc ttatccacat gctcatgccg ttcgcgcagc atcgcgatct ccgcttccac
     1561 aatcgaccac accgtcacct ggctgccgtt ggtgtgcaac actttcgcga gcgccgtccc
     1621 ccagcttccc gctccgatta ccgctacttt tgccatatct tttatttccc ctcctgtttg
     1681 ttcttcttga acgtgaactt attctccgtg ccgtgcagca gacgtccaat gttactcctg
     1741 tgacgccaca gcgccatcac cgtaaagcag gcgctcacca catagaactc cgggagatat
     1801 gcggcatcga tatgcagata tcccatatgt ccgaagatca gtacctgcac cagataactc
     1861 attaccacca gaatcgatcc aagggacaca tatcttgtgc tcgcgatgga accgatgaat
     1921 aagatcaggc agaccggtgc tgccatcggg cacaccgcga gaatcatgcc cgacgtgcag
     1981 gcgattccct ttccaccctt gaacttgaga taaaacggaa aattgtgtcc gagcacagct
     2041 ccgaagccgg catacagttc cagaatctta accgcatccg gataagcgtt ccggaacaaa
     2101 acctttacca gaagaactgc cagaattgcc ttgcacagat ctcccgcaaa cgtaatcagt
     2161 cccgccttga ttccaagcac gcgcagggaa tttgtcgtcc ccgcgttgcc acttccctca
     2221 ctgcggatat caatcccctg gctttttccg tagagatacc ccgtctgaaa cagtccaaac
     2281 agatacccga ttcccaatgc aataaccctt gctcctatca tca
//
LOCUS       DBMDQJ010000400         2321 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16125_length_2321_cov_1.690541, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000400 DBMDQJ010000000
VERSION     DBMDQJ010000400.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16125_length_2321_cov_1.690541"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="ACZO9R_08400"
     CDS             complement(<1..184)
                     /locus_tag="ACZO9R_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913121.1"
                     /translation="MSADYIYAATAAKYGDPLGPTFDMLRRTKSCREIIESASRPIEN
                     LDNRLTFDGFRAWYLER"
     gene            complement(390..1781)
                     /locus_tag="ACZO9R_08405"
     CDS             complement(390..1781)
                     /locus_tag="ACZO9R_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913122.1"
                     /translation="MKLAEFKEKYIGDRAFYKRYLYLALPMIVQNAITNLVSFLDNIM
                     VGQLGTEQMSGVAIVNQLIFVYNLAIFGAVSAASIFGAQYYGKRNHKGHMYSFRFKLY
                     AALLVTGLTILLFLTCGDNLISLYLTDTAGDGATDVALGYGLQYLGIMVVGLVPFAVN
                     QAYATNIKETGQTMVPMVASFVAVGSNALLDYLLIFGIGPFPELGVAGAALATVIARY
                     IEAFIIIVWAHSHTGQNLYLKGAYTGFGIPGEELKAIIVKGFPLMLNEVLWAAGMTAV
                     TQCYSVRGLEVVAGLNIATTITNLFNIIYLQLGSCISIVVGQYLGAGELEEAKDADNK
                     MIVFSVFCCVIVAGLMLVVGGFFPQIYNTTEEIKALATSFIAVSAMIMPFCSFSHASY
                     FTLRSGGKTMVTFLFDSVFTWIVVVPAAFVLARFTGLGIVSVYFLVQATELIKVVIGY
                     RMVRSNVWLVQMV"
     gene            complement(1906..>2321)
                     /locus_tag="ACZO9R_08410"
     CDS             complement(1906..>2321)
                     /locus_tag="ACZO9R_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913123.1"
                     /translation="FRLNKAYDRWKTLSWQEKDKVEFLCNECCWFGCRDRKRCYEAVS
                     RKNLGESGGEHHCAAPDAEGGYLFSKAMANPGFIGTDAIKNIYLPMGFSQFKIEGRGL
                     GSALILEFLLYYMTKPEYQLHVREKIYLDNMLDLF"
BASE COUNT          579 a          702 c          475 g          565 t
ORIGIN      
        1 cccgctccag ataccacgcc cgaaatccgt cgaacgtcaa tcggttgtcc agattttcaa
       61 tcggtctgga agcggattcg atgatctccc ggcagctctt cgttctgcgc agcatatcaa
      121 acgttggacc cagcggatct ccgtatttcg ccgctgttgc ggcataaata taatcagctg
      181 acactctttt tcaaattcat ccatcttctg ctatccttcc gctgccgtat gtgcctgttt
      241 ttccatacac tgctgctctc ccctttatct tagcataaaa caagcggtaa cacacgaatc
      301 tttcatcccg tgataccgcc tattgtttct acattgactt catgctgccg atcatctgtc
      361 agatcttgca accgaacaaa tccattcctt tacaccatct gcaccagcca tacattgctc
      421 cgcaccatgc ggtatccgat caccacctta atcagctcgg tcgcctgcac cagaaagtac
      481 acactcacaa tgccaagtcc tgtaaaacgt gccagtacaa acgcggccgg cacgacaacg
      541 atccacgtga aaacagagtc aaacaggaac gtaaccatcg tttttccccc cgatcgcagc
      601 gtaaagtacg acgcatggct gaacgaacag aacggcatga tcatcgccga tacggcgatg
      661 aagcttgtcg caagcgcctt gatctcctcc gttgtattgt agatctgcgg gaaaaatcct
      721 ccgaccacca gcataagacc tgccacgatc acgcagcaga acacactgaa cacgatcatc
      781 ttgttgtctg cgtcctttgc ttcttccagc tcgccggcgc ccagatactg tccgaccacg
      841 atactgatgc aggaaccaag ctgcagatag atgatgttga acaggtttgt gattgtcgtc
      901 gctatattca gtcccgccac tacctccagt ccacggacgg aatagcactg tgtgaccgcc
      961 gtcatacccg ccgcccacag aacctcgttg agcatcagcg gaaatccctt cacgataatc
     1021 gccttcaatt cctctcccgg aatgccaaat ccggtatagg cgccctttaa atacaggttc
     1081 tgtccggtat ggctgtgcgc ccagacaata atgatgaacg cctcaatgta gcgtgcaatc
     1141 accgtcgcaa gcgcggcgcc ggcaacacca agctccggga acggtccgat tccgaagatc
     1201 agaagataat ctaacagcgc attgcttccc acagcgacaa agctcgcaac catcggcacc
     1261 atcgtctgtc ccgtctcctt gatattcgtc gcatacgcct ggttgaccgc aaacggaacc
     1321 agtcccacta ccatgatccc aagatactgc agtccatacc caagcgccac atccgtcgcc
     1381 ccgtctccgg cggtatccgt cagataaagc gagatcagat tgtccccgca tgttagaaac
     1441 agcagaatgg taagccccgt cacaagcaac gccgcatata gcttaaagcg gaaggaatac
     1501 atatgtccct tgtgattcct cttgccgtaa tactgcgccc cgaaaatact ggctgccgaa
     1561 accgcgccga aaatcgccag attgtataca aaaatgagct ggttcacgat ggcaaccccg
     1621 gacatctgct ccgtgccgag ctgtcccacc atgatgttat ccagaaagct gacgagattc
     1681 gtgatcgcat tctgtacaat catgggcagc gccagatata aatatctttt ataaaacgcc
     1741 cggtctccga tatatttttc cttaaattct gctaacttca tacgttcttt tccttctata
     1801 agatctcttg tgtgtttatc tgatcatctt aacatcttcc cgcaaaaaaa gcactgtttt
     1861 tttgaaaaac agtgccattt cccgcctgcc gcccattgct gccgctcaga acagatccag
     1921 catattgtcc agatagattt tttccctgac gtgcagctga tactcaggtt tcgtcatata
     1981 gtagagcagg aactccagaa tcagcgcact gccaagtccc cgcccctcga tcttgaactg
     2041 ggaaaatccc atgggcaggt agatattttt tatggcgtcc gtaccgataa atcccggatt
     2101 ggccatcgct ttggaaaaca gatagccgcc ctctgcatcc ggagccgcgc agtgatgctc
     2161 cccgccgctt tctccgagat ttttgcggct gacggcttcg taacagcgct tcctgtccct
     2221 gcagccaaac cagcagcatt cattgcacaa aaactccacc ttgtcttttt cttgccaaga
     2281 cagtgtcttc catcggtcat aggctttatt taagcgaaaa t
//
LOCUS       DBMDQJ010000401         2300 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16316_length_2300_cov_1.683947, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000401 DBMDQJ010000000
VERSION     DBMDQJ010000401.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16316_length_2300_cov_1.683947"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="ACZO9R_08415"
     CDS             complement(<1..607)
                     /locus_tag="ACZO9R_08415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0913124.1"
                     /translation="MILNLQNIYKDYQQEKLVVPVLKDVSLTVEEGEYVAIMGPSGSG
                     KTTLMNIIGCLDRPTSGTYELAGENVLKLKDRELSDLRLKSIGFVFQSFQLMPRESAV
                     ENVALPLSYAGVRKKERRSRATKALERVGLGDRVNFRPTQLSGGQKQRVAIARAMVNH
                     PKILLADEPTGALDSKSGEQIMELFDSLNEEGVTIVMITHDP"
     gene            complement(604..1977)
                     /locus_tag="ACZO9R_08420"
     CDS             complement(604..1977)
                     /locus_tag="ACZO9R_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR0913125.1"
                     /translation="MGKKSVGILIGVAAVAAVLGGTGYYFRDDIRQMIPIFDDGSSED
                     KVYVEKVSRIMNQYAGVSNRYNGVVETQDSYEVNVDSSRTISEIKVEVGDEVEEGQTL
                     VTYDTSDLTMKIEQAKLELEGIQNEIDNYNKQIDTLTKEMEKVDESERYDYTTQIQNI
                     QNSIAQKQFDMESKKLEISKEQKQVSSSSVVSKVAGVVKEINEKGVDSNGNSAPFMTI
                     LQTGEYRIKGSIDEQNVWMLSEGQEVVIRSRVDGTKTWSGTIGKIDTESPQQGNDNSY
                     YSTSSAGDTQSASKYPFYVDLDSVDGLILGQHVYIELDQGQEEVKEGLWLYGSYIVQD
                     EDTPYVWAANEKDRLEKRYIELGEYDADMDEYEIVSGLAEDDYIAWPMAGLYEGVTTV
                     TDEAEVDYSSPLYNQPADENLYDTEGVYDTELLYNVLDSVYDTELPDEMYDSMDAGEG
                     TEAEVAE"
BASE COUNT          480 a          724 c          466 g          630 t
ORIGIN      
        1 tgggatcatg cgtaatcatg acaatagtaa cgccctcttc gttcaagctg tcaaaaagct
       61 ccatgatctg ctcgccggat ttggagtcca gggctcctgt cggctcatcc gccagcagaa
      121 tcttaggatg gttgaccatc gcgcgggcga tggcgacacg ctgcttctgt ccgccggaaa
      181 gctgtgtcgg tctgaaattg acacgatccc cgagtcctac tctctcgagc gctttcgtcg
      241 cgcggctccg tctctccttt ttgcgcactc cggcatagct gagcggaagc gccacatttt
      301 ccacggcact ctcccgcggc atcagctgaa agctctggaa cacgaacccg atacttttta
      361 agcggagatc cgagagctcc ctgtctttta atttgagcac gttctcccct gccagctcgt
      421 aagtaccgct cgtcgggcgg tcgagacacc cgataatatt catcagtgtg gtcttaccgg
      481 aaccggacgg tcccataatg gcgacatact ctccttcctc gaccgtcagc gatacatcct
      541 ttaacaccgg cacaaccagc ttctcctgct gatagtcttt gtagatattc tgcagattca
      601 gtatcattct gccacctccg cctctgtccc ttctccggca tccatgctgt catacatttc
      661 atccggcagt tctgtatcat agacgctgtc caacacatta tagagcagtt ccgtatcata
      721 gacaccctct gtatcgtaca gattctcgtc cgccggctga ttgtaaagcg gagaagaata
      781 atccacttcc gcctcgtcgg tcacagtcgt cacaccctca tagagaccag ccatcggcca
      841 ggcaatgtaa tcgtcctcgg caagccctga tacgatctca tactcatcca tatccgcatc
      901 gtactctccc agctcgatgt agcgcttctc cagacggtct ttctcattag ccgcccacac
      961 atacggggta tcttcgtcct gcacgatgta ggagccgtac agccacagac cttccttcac
     1021 ttcctcctgt ccctgatcca gctcgatgta cacatgctgc cccaggatca gtccgtccac
     1081 cgagtcgaga tccacataga acggatactt ggacgctgac tgtgtatctc ccgccgaaga
     1141 agtgctgtaa tagctgttat cgttcccctg ctgcgggctc tccgtgtcaa ttttcccgat
     1201 cgttccgctc cacgtcttcg tgccgtccac acgcgagcgg atcacaacct cctgtccctc
     1261 ggaaagcatc cagacattct gctcatcgat gcttcccttg atccggtact ctccagtctg
     1321 taagatcgtc ataaacggcg cgctgttccc gttgctatcc acgcctttct cattgatctc
     1381 cttgaccact cccgccacct tgctcacaac ggaagaactg ctgacctgtt tctgctcctt
     1441 ggagatctca agcttcttgc tctccatatc gaactgcttc tgtgcgatgg agttctggat
     1501 attctgaatc tgcgtggtgt aatcgtagcg ttccgattcg tccaccttct ccatctcttt
     1561 cgtcagtgtg tcgatctgtt tgttgtaatt atctatctca ttctggatgc cttccagctc
     1621 cagcttcgcc tgctcaatct tcatggtaag gtcactggtg tcgtaagtga ccagagtctg
     1681 tccctcctcg acctcatcgc cgacctccac cttgatctcg ctgatcgtcc gggaagaatc
     1741 cacattcacc tcgtatgaat cctgcgtctc caccacaccg ttataccggt tggaaacacc
     1801 tgcatactga ttcataatgc gggatacctt ttccacatag accttgtcct ctgaacttcc
     1861 atcatcaaag atcggtatca tctgcctgat atcatcccgg aaataataac cggttcctcc
     1921 cagaacagca gccactgctg ccacaccgat cagaattcca acgctctttt tgcccatagc
     1981 tcctcctctg ccccagacgg gttactttct tttctttatt ctatcatctt ttcagacccg
     2041 atacatagcg tttaattctt aataattttt gtagaataga acattaaaaa agcctccgcg
     2101 catattgaac actcacaata tgcacggaag cctagtctac acactttgaa agattttatt
     2161 ttaacggata ttttgcggtg agctccgcca caatcgctct ggcgcgatct gccgccggct
     2221 ctcccttctt gatcatcatg gcaatcgcct ccgcaatctg atccatatcc ccggtattga
     2281 atccgcggga tgtcaccgcc
//
LOCUS       DBMDQJ010000402         2298 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16331_length_2298_cov_1.506145, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000402 DBMDQJ010000000
VERSION     DBMDQJ010000402.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16331_length_2298_cov_1.506145"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1836
                     /locus_tag="ACZO9R_08425"
     CDS             <1..1836
                     /locus_tag="ACZO9R_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0913126.1"
                     /translation="LSDNPDFQKEPYILTDSFYTDDVALVGRNGQEYDVDADDVSYRL
                     AIPASYVGLEHYIQMNAPQFEICEEKNLEDCLAMVLDGKADFAAQNVSVLTPYLANPY
                     YEELAAVPTFFMEENTGIVGLDSEEHRMLIKILNKCIGMISEKEIAQFKVDHTLATAY
                     EPTLSDMVYKFRYPIAVIGILLLQIVAWMVAFELLRRRNYHKLEEKNEQLAVAVAQAN
                     SANEAKGQFLARMSHEIRTPINAIVGLTEIARHRSDEGGGVEECLDKIDTSSKVLLAI
                     VNDVLDMSAIESNKIKIAQAPFSLKEVLDSISAVYGTQCEQKKIAFTVEMDEVQVEQV
                     QGDVLRLNQVLLNLISNAYKFTPEGGSIRVTVSELPMQQEQFFYKFVVTDTGEGMSEE
                     MQERLFLPFEQEEAETAQHHGGSGLGLSIAKNLVELMGGSISCQSKKGVGTTFTVSLP
                     LAHAVDEKKQKAEETGEGEAEEDASESYDFGGRRILLAEDTEMNAEIVTELLELVNMH
                     VDHAWNGEEAVERYMESAPGTYMAVLMDVQMPGMNGYEAAKAIRAQKSSRPDAGEIPI
                     YAMTANAYTEDISAALNAGMNGHIAKPVDTAALYRLLKKSMEETG"
     gene            1833..>2298
                     /locus_tag="ACZO9R_08430"
     CDS             1833..>2298
                     /locus_tag="ACZO9R_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0913127.1"
                     /translation="MREKICTFSGIHISPADPRPEEICIEDIAHALSLMTRANGHFPV
                     FYSVAQHSLDCAAMAQAEGRSSREVLACLLHDASEAYLSDITRPVKGCLPEYRRIEQV
                     LQEMIYEKYLIAPPTEREREIVKRMDDTCLYYEFEHFTGEKLFAFCPDRSGRA"
BASE COUNT          616 a          478 c          699 g          505 t
ORIGIN      
        1 ctctcggaca atcctgattt ccagaaggag ccctatattc tgacggacag cttttatacg
       61 gatgacgtcg cactggtcgg aagaaacgga caggaatatg atgtggatgc ggacgatgtt
      121 tcataccgtc tggcaattcc ggcaagctat gtcgggctgg aacattatat ccagatgaat
      181 gcaccacagt ttgagatctg tgaggagaag aacttagaag actgtctggc catggttttg
      241 gacggaaaag cagattttgc ggcgcagaat gtcagcgtgc tgacgccgta tctggcaaat
      301 ccctattatg aggaacttgc tgctgtcccg acttttttta tggaggaaaa tacgggaatc
      361 gtcgggttgg acagcgagga acaccgcatg ctgataaaga ttttgaacaa gtgcattggg
      421 atgatatcag aaaaagagat tgcccagttt aaggtggatc acacgctggc aaccgcctat
      481 gagccgacac tctccgatat ggtatacaag ttccgttatc cgatagccgt gatcggcatc
      541 cttctgctgc agattgtcgc atggatggtt gcatttgaac ttttgagacg cagaaattac
      601 cacaagctgg aagagaaaaa cgagcagctc gctgtggcgg tggcacaggc caacagcgcc
      661 aatgaggcaa agggacagtt ccttgcgcgg atgagccatg agattcgtac gccgatcaat
      721 gccattgtgg ggctgactga gatcgcaaga caccgcagcg atgaaggggg aggggtagaa
      781 gagtgtctgg ataagatcga tacctcatcc aaagtgctgc ttgcgattgt aaatgacgta
      841 cttgatatgt ctgcgatcga gagcaacaag attaaaattg cgcaggcacc gttttcctta
      901 aaagaggtgt tggattcaat ttcagcagta tatggaacgc agtgtgagca gaaaaagatt
      961 gcctttaccg tggagatgga cgaggtacag gtggagcagg tccaaggtga tgtgctgcgc
     1021 ctcaatcagg tattgctgaa tctgatttcc aacgcctata agtttacgcc agagggcggg
     1081 agtatccgcg tgacggtcag cgaactgcca atgcagcagg aacagttctt ctataaattt
     1141 gtggtgacgg ataccggcga gggaatgagc gaggaaatgc aggagcggct gttcctgccg
     1201 tttgagcagg aggaggccga gacggcacag catcacggcg gaagcggact cggactttct
     1261 atcgccaaaa atctggtgga actgatgggt ggatcgatct cctgccagtc gaagaagggt
     1321 gtcggaacaa cctttacagt atccctgccg ctggcacacg cagtggatga gaaaaagcaa
     1381 aaagcagaag agacggggga aggtgaagca gaagaagatg cgtcagagtc ttatgatttt
     1441 ggcggcagac ggattcttct tgcggaagat accgagatga acgcggagat tgtcacggag
     1501 ctgctggagc ttgtgaacat gcatgtggat catgcgtgga acggtgagga agcagtggag
     1561 cggtatatgg aatcagcacc gggaacctat atggcagtcc tgatggatgt ccagatgccg
     1621 ggaatgaatg gctatgaagc ggcgaaagcg atccgcgcac agaagagcag ccgcccggat
     1681 gccggggaga taccgatcta tgcaatgact gcaaatgcat acaccgagga catcagtgcg
     1741 gcgttgaatg cgggaatgaa cggacatatt gcaaagccgg tggataccgc cgcgttgtat
     1801 cgtcttttga agaagagcat ggaggaaacg ggatgagaga gaagatttgc acattttccg
     1861 ggatacatat atcaccggca gatcctagac cggaagaaat ctgtatcgag gatattgcac
     1921 atgcgctgtc gttaatgaca cgtgccaacg ggcattttcc ggtattttat tctgttgcgc
     1981 agcatagcct ggactgtgct gcaatggcgc aggccgaagg gcgcagcagc agggaggttc
     2041 tggcgtgtct gctgcacgat gcgagcgagg catatctgtc ggatatcacc aggccggtga
     2101 agggctgtct gcccgaatac cgcaggattg agcaggtgct tcaggagatg atctatgaaa
     2161 aatacctgat tgcgccgccg acggagcggg agcgcgagat tgtaaagcgc atggatgata
     2221 cctgcctgta ttatgaattt gaacatttca cgggagaaaa attatttgca ttttgcccgg
     2281 atcgatcggg tagagcat
//
LOCUS       DBMDQJ010000403         2289 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16408_length_2289_cov_1.513711, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000403 DBMDQJ010000000
VERSION     DBMDQJ010000403.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16408_length_2289_cov_1.513711"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="ACZO9R_08435"
     CDS             <1..90
                     /locus_tag="ACZO9R_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR0913128.1"
                     /translation="VAIEEGATMVRVGTGIFGARNYKKSNESH"
     gene            100..645
                     /locus_tag="ACZO9R_08440"
     CDS             100..645
                     /locus_tag="ACZO9R_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HIR0913129.1"
                     /translation="MSVLDKFLNVMRLNPDDDDDFYNEDYDYDDDYEEDEPVKSKSSF
                     RKDKRKAEEDDYEEDHSIHEKAVKSSPKVTPIRPVKKQGGSGMEVCVIKPTSVEDARE
                     ITETLLNNRTVVLNVEGLDVEIAQRIIDFTSGSCFAISGNLQKISNYIFIITPASVDI
                     SGDFLSLVDTFQSTRSVQTDF"
     gene            651..1403
                     /locus_tag="ACZO9R_08445"
     CDS             651..1403
                     /locus_tag="ACZO9R_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH family RNA-binding protein"
                     /protein_id="HIR0913130.1"
                     /translation="MNEQDLCQKRLLDLSRQADRKGIVLFSDFLNLNELNIYHQSEKL
                     FATRTECFGGIPCAERQMIAFLPDALYYDWEYPISALKITPAYPKFAEKLGHRDILGS
                     LMQLGIDRCKLGDILVGEQEAYLLCEETVADYFAEHLEKIRHTLVKVEPCSLCDLKAT
                     QEFQSQDGIVTSDRIDAVIACVYHLSRSQASLLLRQEKVFVSGKLMQNPSYTCHADDI
                     ISVRGYGRFIYRGEYGTTGKGRLKIHYDLYAN"
     gene            1416..2204
                     /gene="aroB"
                     /locus_tag="ACZO9R_08450"
                     /pseudo
     CDS             1416..2204
                     /gene="aroB"
                     /locus_tag="ACZO9R_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
BASE COUNT          596 a          509 c          584 g          600 t
ORIGIN      
        1 gttgccattg aagaaggagc gacgatggtg cgtgtcggaa ccggaatttt cggtgcacgc
       61 aattacaaaa aaagcaatga atcgcattag gagtataaca tgagtgtttt agataaattt
      121 ttaaatgtca tgcgtcttaa tccggacgat gacgacgatt tttacaatga agattatgat
      181 tacgatgatg attatgaaga ggatgaaccg gtaaaatcga agagttcttt ccgcaaggac
      241 aagaggaagg cggaggagga tgattatgaa gaagatcatt ctatccacga gaaagccgtc
      301 aagagttctc cgaaggtcac accgattcgc ccggtcaaga agcagggagg aagcggcatg
      361 gaagtatgtg tcatcaaacc aacctcagtg gaagatgcca gagagattac agagacgctt
      421 ctcaataacc gcacggtcgt attgaacgtt gagggactgg acgtcgagat cgcacagcgg
      481 attatcgatt ttacatcggg ttcctgcttt gcaatcagcg gtaatcttca gaagatctcc
      541 aactatatct ttatcattac acccgcatcg gttgacattt ccggcgattt cttaagtctg
      601 gtagacacgt ttcagtcgac acgcagtgtc cagaccgatt tttagagtct atgaatgaac
      661 aggatctttg tcagaagcgt ctgcttgatc tctccagaca ggcggatcgc aagggaatcg
      721 ttctattcag cgatttcctc aatttaaatg aattgaatat ataccaccag tccgagaagc
      781 tgtttgcgac gaggaccgaa tgttttggcg gtattccatg tgcagagcgt cagatgattg
      841 cctttcttcc tgatgctctt tattatgatt gggaatatcc catttccgcc ttaaaaatta
      901 ccccggctta tcccaaattt gccgagaagc ttggacatcg ggacatcctc ggatcgctga
      961 tgcagcttgg cattgaccgc tgcaaattag gcgatattct ggtaggggag caggaagcct
     1021 atctgctgtg tgaggagacc gttgcggact attttgcgga gcatcttgaa aagatacgcc
     1081 acaccctcgt gaaagtcgag ccgtgcagcc tgtgtgatct taaggccaca caggaattcc
     1141 agtcacagga cgggatcgta acctcagacc ggattgacgc ggtcatcgcg tgcgtttatc
     1201 atctgtcccg cagtcaggca tcgcttttgc tacgccagga gaaggttttt gtcagcggaa
     1261 agctgatgca gaatccatcc tatacctgcc atgcggatga tattatttct gtccgcggtt
     1321 acggacgctt tatctatcgc ggggaatacg ggacgaccgg aaagggacgg ctcaaaatac
     1381 attacgacct ctatgcaaac tgaaaggagt ttttcatggc aaaatcgatt ccggtaaaag
     1441 caaacgacag tatcatttat catattgaac tggttcatga tttttcaatg ctccctgcgt
     1501 ttctgcagga acttggctac gggcacagtc agaaaatctg cattgtgacc gattccaacg
     1561 ttgcgccttt gtatgcgagg gacgtacagg atctgttgga aaaagagttt gacacggtca
     1621 ttcttcatac gtttgaggca ggagagcaca ataaaaatct tgagacggtg aacgctttgt
     1681 accagacgct gatcgagcag cattttgacc gtcaggatct gcttgtcgct ttaggcggcg
     1741 gcgttgtcgg cgatcttacc ggttttgcgg cggcgaccta cctgcgcggc attgatttta
     1801 tccaggtgcc gaccacgctg ctttcccagg tagacagcag catcggcggg aagaccggcg
     1861 tggactttat gcagtacaaa aatatggtgg gtgcttttta tcagcccaaa ctggtctata
     1921 tgaacctgtc cgtcctgcaa tcccttcctt ccaggcagat catttccggg atgggagaga
     1981 ttataaaaca cggtcttatc gaggatgccg cttactatga atggctgatg gcacatcatg
     2041 atgccatttt ggcactggag ccggctgtca tggaagagat gatcgcccga agctgtgaga
     2101 tcaagcgcga agttgtagag cacgacccga cggagaaggg agagcgcgca ctgttgaact
     2161 ttggtcatac catcgggcat gccatagaga aattatgcgg tttttgtcat cttcctctat
     2221 tctttccctt atctgctgca ttcttctgtc aagctgactg attaaatctg cacaaaaata
     2281 caattcttc
//
LOCUS       DBMDQJ010000404         2284 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16461_length_2284_cov_1.401283, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000404 DBMDQJ010000000
VERSION     DBMDQJ010000404.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16461_length_2284_cov_1.401283"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            35..895
                     /gene="speE"
                     /locus_tag="ACZO9R_08455"
     CDS             35..895
                     /gene="speE"
                     /locus_tag="ACZO9R_08455"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HIR0913131.1"
                     /translation="MELWFSDNQTDHVKVSVKVEKQLFGEQTDFQRIDVFESREFGRF
                     LSSDGSIVFSEKDEFIYDEMIVHVPMAVHPHVKKVLVIGGGDGGVARELSYYEEIEQI
                     DVVESDRTFVDVCRKFFPDNACGLEDPRVNIYYEDGLRFLRTKHDAYDLIINDAIDPL
                     GHTAGLFTKEFYGNCYRALKEDGIMVYQHGSPFYDEDEESCRVMHRKASHSFPISRVY
                     QAHIPTCSSGYWLFGFASKKYHPLTDLNVERWKARGIKTWYYTTNLHKGAFMLPKYVE
                     DMLEEEEGRR"
     gene            1029..>2284
                     /locus_tag="ACZO9R_08460"
     CDS             1029..>2284
                     /locus_tag="ACZO9R_08460"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012744094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR0913132.1"
                     /translation="MGRLLIIGCGGVAGVAIHKCCQNSRTFSEICIASRTKEKCDALK
                     EKLQGTTDTVITTAKVDADNVDELIALIRDYKPDAVLNVALPYQDLTIMDACLACKVD
                     YIDTANYEPEDTDDPAWRAIYEKRCKEAGFTAYFDYSWQWAYQKRFTDAGITALLGSG
                     FDPGVTSVFTAYALKHYFDEIEYIDILDCNGGDHGYPFATNFNPEINLREVSANGSYW
                     EDGHWVETKPMEIKREYNFPQVGEKDMYLLHHEEIESLAKNVPGVKRIRFFMTFGQSY
                     LTHMQCLEDVGMLSTSPIQYEGREIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGK
                     KDGKEKTIYIYNVCDHQECYREVGSQAISYTTGVPAMIGAALVVDKVWDKDGVFNIEE
                     FDPDPFMDMLNQYGLPWVVDENPKTVA"
BASE COUNT          599 a          496 c          679 g          510 t
ORIGIN      
        1 cgtgcgatac ggtctgggga aggtggaaga gagcgtggag ttatggtttt cggataatca
       61 gaccgatcat gtgaaggtgt cggtcaaagt ggagaagcag ctgttcgggg agcagacgga
      121 ttttcagagg atcgacgtgt ttgaatccag ggaattcggc agatttttaa gctccgatgg
      181 cagcatcgtc ttctcagaaa aggatgagtt catctacgat gagatgattg tgcatgtgcc
      241 gatggcggtg catccgcatg tgaaaaaagt gctggtcatc ggtggcggag acggcggtgt
      301 ggcgagagaa ctttcctatt atgaggagat cgagcagatc gacgtcgtgg aatccgaccg
      361 gacattcgtg gatgtgtgca ggaagttttt cccggacaac gcctgcggac tggaagatcc
      421 gcgcgtcaat atctattacg aggatggact gcgctttctg cgcacaaagc atgatgccta
      481 cgatctgatc atcaatgatg cgatcgatcc gctcgggcat acggcgggat tgttcaccaa
      541 ggaattttat gggaactgtt accgcgcatt aaaagaagac ggcattatgg tctaccagca
      601 cggcagtcct ttttatgacg aggatgagga gtcctgccgc gtgatgcacc gcaaggcgag
      661 tcacagtttc ccgatcagcc gtgtgtatca ggcgcacatt ccgacctgtt cctccgggta
      721 ctggctgttc ggatttgcgt caaaaaaata tcacccgctg acggatctga atgtggagcg
      781 ctggaaagca cgcgggatca agacctggta ttatacgacc aatcttcata agggagcgtt
      841 tatgctgcca aaatatgtcg aggatatgct tgaggaagag gaaggcagaa ggtaaatggc
      901 agatgcggga cagaacggac tctgacggga cagtcagcgg agcgttctgt catccgttgg
      961 cagaatgata taaataatat aaaaatagaa aatacagccg caggacggca gaatgagagg
     1021 ataagtagat gggaagatta ttgattatcg gatgtggcgg agtagcaggg gtggcaattc
     1081 acaaatgctg ccagaacagc aggacttttt cggagatctg tattgcaagc cgcacaaaag
     1141 aaaaatgtga cgcgttaaag gaaaagctgc agggaacgac ggatacagtg atcacgacag
     1201 cgaaggtgga tgcggataat gtggacgaac tgatcgcact gatccgtgac tacaagccgg
     1261 atgccgtgtt aaatgtggcg ctgccttacc aggatctgac gattatggat gcctgcctgg
     1321 catgcaaggt ggactacatc gataccgcga actacgaacc ggaagatacc gacgacccgg
     1381 cgtggcgtgc aatttatgag aagcgctgca aggaggcggg ctttaccgcg tacttcgatt
     1441 attcctggca gtgggcatat cagaaacgct ttacagatgc cggcatcacg gcgcttcttg
     1501 gcagcggctt tgacccgggc gtgacgagcg tgtttaccgc ctatgcgctg aagcattatt
     1561 tcgatgagat cgagtacatc gatatcttag actgcaacgg tggagaccac ggttatccgt
     1621 tcgccaccaa tttcaacccg gagatcaacc tccgcgaggt atccgcaaac ggctcctact
     1681 gggaggacgg acactgggta gagacaaaac cgatggaaat taaaagagaa tataatttcc
     1741 cacaggttgg cgagaaagac atgtatctgc ttcatcatga ggagatcgag tctctggcaa
     1801 agaatgtgcc gggagtcaag cgcatccgct tcttcatgac cttcggtcag agctatctga
     1861 cccatatgca gtgtctggag gatgtcggaa tgctctccac ttccccgatc cagtatgagg
     1921 gaagagagat cgtgccgatc cagttcttaa aggcattgct tccggacccg gcgtcactgg
     1981 gtcctagaac cgttggcaag acgaacatcg gctgtatttt taccggcaag aaggatggca
     2041 aggaaaagac gatctatatc tataacgtct gcgatcacca ggagtgctac agggaggttg
     2101 gctcacaggc gatctcctac acgacaggag ttccggcaat gatcggtgca gcgcttgttg
     2161 tagataaggt gtgggataag gacggcgttt tcaatatcga ggagtttgat ccggatccgt
     2221 ttatggatat gctcaaccag tacggactgc cgtgggttgt cgatgagaac cccaagacgg
     2281 tggc
//
LOCUS       DBMDQJ010000405         2282 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16477_length_2282_cov_1.399358, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000405 DBMDQJ010000000
VERSION     DBMDQJ010000405.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16477_length_2282_cov_1.399358"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(85..1419)
                     /locus_tag="ACZO9R_08465"
     CDS             complement(85..1419)
                     /locus_tag="ACZO9R_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913133.1"
                     /translation="MNETFMKEKPILPLLTSMALPMVISMMVNALYNIVDSLFVARIN
                     ENAMTALSLVFPIQNFVNALAIGFGVGINALIAYFLGAGDKKGADRAATQGLVLALIH
                     GVVVTVLSISIMPAFLDMFTNDVQVYQMGMEYAVIVFAFSTITMANLSFEKIFQSVGR
                     MKVSMIGLLCGSITNIILDPLLIFGIGIFPRLGIAGAAIATAIGQLCTLLLYLTVYNK
                     RPIPVKIRKEYLAFHKETDHRLYSIGVPAILNLALPSLLVSVLNGILAAYSQIYVVIL
                     GIYYKLQTFLYLPANGIVQGMRPLIGYNYGAGEQKRVKKIYEITLAMSAVIMALGTIL
                     CLVASRGLMELFTENAETIAAGGTALRIISAGFIISSVSVTASGALEGLGKGTQSLVI
                     SLFRYVILMIPAAFVLCRIFGPTGVWHAFWITEAVTSVIAAYIYHKAVGSRK"
     gene            1740..2048
                     /gene="rplU"
                     /locus_tag="ACZO9R_08470"
     CDS             1740..2048
                     /gene="rplU"
                     /locus_tag="ACZO9R_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR0913134.1"
                     /translation="MYAIIATGGKQYKVSEGDIITIEKLGVDAGEKVTFDQVLAVSGD
                     DMKVGNPTVAGATVEASVIKEGRAKKVIVYKYKRKTGYHKKNGHRQSFTQVKIEKING
                     "
     gene            2066..>2282
                     /locus_tag="ACZO9R_08475"
     CDS             2066..>2282
                     /locus_tag="ACZO9R_08475"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF016233.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HIR0913135.1"
                     /translation="MITITILQDHEDAVGFHCIGHSGYAEAGSDIICAGVSALVINTI
                     NSIETFTEDSYHIDTEETSGLIDFRLDQ"
BASE COUNT          676 a          485 c          572 g          549 t
ORIGIN      
        1 ctacatgctg tgttttctgg catattactt atcacttcaa aatgaaatgt ctgcttaaag
       61 caccagcctc ttttttgaca gatgttattt tctgcttccg actgccttat ggtagatata
      121 agcggcgatg accgaggtga ctgcttcggt gatccagaaa gcatgccata ctccggttgg
      181 tccaaagata cggcacaata caaaggctgc cgggatcatg agaattacat agcggaacag
      241 cgatatgaca agggactgcg ttccttttcc aagcccctcc aaggcgccgg aggctgtgac
      301 ggacacggag gagatgataa agcctgcact gatgatgcgc agtgcggttc ctccggctgc
      361 gatcgtctcg gcattttcgg tgaacagttc catcagaccg cgcgatgcca ccaggcataa
      421 aattgtgccg agcgccataa tgacagcact catcgcaagc gtaatctcat agatcttttt
      481 cacgcgcttc tgctcgccgg caccgtaatt atagccgatc agcggacgca tgccctggac
      541 aatgccgttt gccggaagat agaggaaggt ctgtaatttg taatagattc ccagaatgac
      601 cacatagatc tgggagtaag ctgccagaat accgtttaag acggatacta acagcgatgg
      661 cagggcaaga tttaagatgg caggaactcc gatcgagtag agacgatggt cggtttcctt
      721 atgaaaagca aggtattctt tgcggatttt gaccgggatc gggcgcttgt tgtacaccgt
      781 caggtaaaga aggagggtac acagctgacc gatcgctgtg gcaatcgctg cgccggcgat
      841 accaagcctc gggaaaattc cgatgccgaa aatcaggagc gggtctaaga taatattagt
      901 aatacttccg cacagcaggc cgatcatgga tactttcatg cgtcccacgg actggaagat
      961 tttctcaaag gaaagattcg ccatggtgat tgtcgaaaac gcaaatacga tcaccgcgta
     1021 ctccattccc atctgatata cctgcacatc gttggtgaac atgtccagga aagccggcat
     1081 gatggagatg cttaacacag tgacgacaac gccgtggatg agtgccagaa caagtccctg
     1141 tgtggcggcg cggtcggcgc cttttttgtc gcctgctccg agaaaatagg caatcagtgc
     1201 attgatacca acgccgaatc cgatggcaag cgcgttgaca aaattctgaa tcgggaagac
     1261 gagggacagc gctgtcatgg cattctcatt gattctggcg acgaacagac tgtctacaat
     1321 attataaagt gcgtttacca tcatggatat gaccatcgga agcgccatgg aggtaagcag
     1381 cggcaggata ggtttttctt tcataaatgt ttcgttcatg tggtatttcc tcctatataa
     1441 tagtctgggt ttattgtatc caggataaaa cagaaaacgc cacacaatga ctcaaaagaa
     1501 tcattgtgtg gcgaaaaaaa gtcagacttg aaaagtccac accaagtttt agcgaagaga
     1561 gtatagcacg atccatagga aaaagtcaag aattttggca aaaaagtcaa aaaatctgtt
     1621 gacgaaacca aactgctatg ctattataat cgagtatgcc gcacagtgag gtctcaaagt
     1681 ccgtccggat tgcccgggcg acaccacaac tggcgagtaa tgataatagg aggtgccata
     1741 tgtacgcaat tatagcaaca ggtggtaaac agtacaaagt atccgaaggc gatatcatta
     1801 ccattgaaaa gcttggagtt gatgctggtg agaaggttac ttttgatcag gtattagcag
     1861 tatccggaga cgatatgaag gttggaaacc caacagtagc cggagcaact gttgaagctt
     1921 ctgtcatcaa agaaggcaga gcaaagaaag taatcgttta caagtacaag agaaaaaccg
     1981 gttatcacaa gaagaacgga cacagacagt cgttcaccca ggttaagatt gaaaagatta
     2041 atggataatc gataggaatt gttatatgat tactataacg attttacagg atcatgaaga
     2101 tgcagtggga tttcactgca tcggtcattc cggatatgcg gaagcaggaa gtgatattat
     2161 ctgcgcaggt gtttcggcac ttgtgataaa tacgatcaat tccatagaga ctttcacaga
     2221 ggattcttat catattgata cagaagagac atccgggctg attgatttca gacttgatca
     2281 ga
//
LOCUS       DBMDQJ010000406         2269 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16586_length_2269_cov_1.615314, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000406 DBMDQJ010000000
VERSION     DBMDQJ010000406.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16586_length_2269_cov_1.615314"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="ACZO9R_08480"
     CDS             <1..1038
                     /locus_tag="ACZO9R_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HIR0913136.1"
                     /translation="GSANVKMARACLTEGITDGIAFKSAQSQKDAQSLRFTERQADAI
                     LEMRLYKLIGLEIEALMKEHEKTLANIAEYNEILSNRAAMAKVIIRELEAYKKEYGRP
                     RRTVIDNLKEAVVEEKKIEEMDVVFLLDRFGYAKTIDVAAYERNREAADAENRYVLTC
                     KNTDKICIFTNKGQMHLLKVLDLPFGKFRDKGTPIDNLSNYNSSEENFVYITNLGAIC
                     NHRLIFGTKTAMLKIVDGSEFDVAKRTTAATKLTDGDEVLFVHPVAGNETIVMQSERD
                     FFLRIAVDTIPEKKKGAVGVRGMRLADGDALTAIHLLDDGESAQTEVKGKPVMLNRLH
                     VAGRDTKGTKK"
     gene            1041..1721
                     /locus_tag="ACZO9R_08485"
     CDS             1041..1721
                     /locus_tag="ACZO9R_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HIR0913137.1"
                     /translation="MIKAVLFDLDGTIINSEEGITKCVQYALRAYGIDEPDRTKLRCF
                     IGPPLDPVFREKYGMTEAEAWQSVEKYRERFDVKGIFECSLYDGVREAIARFKEHGYL
                     LALASSKPETACRRILEHFDLLGYFDEVVGSTLDGSISTKAEVLEELGRRMAGSHIGK
                     NELCLIGDTKYDAAGAKAFGIRCIGVSYGFGTREELLAAGAEAVFDRIEEVETYIEND
                     AAGEGEAE"
     gene            1718..>2269
                     /locus_tag="ACZO9R_08490"
     CDS             1718..>2269
                     /locus_tag="ACZO9R_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR0913138.1"
                     /translation="MKTTGAYKIWRIIYPIGIYYVVSSLAYFFLTLLLGAGEESYMLR
                     QMLCAAATIPFVWMHYGQDRAARETVYGRRGIRPDARFFCTVAGSLLAGASLGMAVNN
                     VIAMTPLIEASAGFQSANQAFFAGGVVFELLGSCLVIPLAEELLYRGVVERRLSLLCG
                     SAPAIVLSAVIFGVMHWNVVQFLY"
BASE COUNT          530 a          513 c          737 g          489 t
ORIGIN      
        1 ggcagtgcga acgtcaagat ggcgagagcc tgtctgaccg agggaatcac cgatgggatt
       61 gcctttaagt cggcgcagtc ccaaaaggac gcgcagagcc tgcgctttac ggagcgtcag
      121 gcggatgcca tcttagagat gcgtctgtac aagctgatcg gactcgagat cgaggcgctg
      181 atgaaagagc acgagaagac gcttgccaat attgcggagt acaatgagat cttaagcaac
      241 cgtgcggcga tggcaaaggt gattatccgc gagctggaag cttacaaaaa ggagtatggc
      301 agaccgcggc gcactgtcat tgacaatctc aaagaggcgg ttgtcgagga gaagaagatt
      361 gaggagatgg acgtggtatt cctgctggat cgctttggct atgcgaagac gatcgacgtg
      421 gcggcgtatg aacgcaaccg cgaggcggcc gatgcggaga accgctatgt gctgacctgt
      481 aagaataccg ataagatctg catttttacg aacaagggac agatgcatct gctcaaggtg
      541 ctggatctgc cattcggcaa gttccgcgac aagggaacgc cgattgacaa tctgagcaat
      601 tataacagca gtgaggagaa ctttgtctat atcacgaatc tgggagcgat ctgcaaccac
      661 aggctgatct ttggcacgaa gacggcgatg cttaagattg tggacggaag cgagtttgat
      721 gttgcaaagc gcacgaccgc ggcgacgaag cttaccgacg gggacgaggt gcttttcgtg
      781 catccggttg ccgggaatga gacaattgtc atgcagtctg agcgcgattt cttcctccgg
      841 attgcggtgg ataccattcc ggagaagaaa aaaggtgcgg tcggcgtccg cggcatgcgg
      901 cttgcggacg gagatgcgct taccgcgatt catctgcttg acgatggtga gagcgcccag
      961 acggaggtaa aaggaaagcc tgtgatgtta aaccggctgc atgtggcggg ccgcgacacg
     1021 aaaggaacga agaaataacg atgataaaag ctgtattatt tgatctggac ggaacgatca
     1081 tcaattccga ggagggcatc acaaagtgcg tgcaatacgc actgcgcgcc tacgggatcg
     1141 atgagccgga ccgcacgaag ctgcgctgct ttatcggacc gcccttagat ccggtatttc
     1201 gcgaaaagta tggcatgacg gaagcggaag cgtggcagtc tgtggaaaag tacagagagc
     1261 ggtttgacgt aaagggaatc tttgagtgca gcctctatga cggcgtcagg gaagcgatcg
     1321 cgcgcttcaa ggagcatggt tatctgctcg cacttgcgtc ctcaaagcct gagacggcgt
     1381 gcagaagaat attagagcac tttgatctgc tgggatattt tgatgaggtg gtaggttcta
     1441 cgctggatgg ttcgatcagt acaaaggcgg aagtgctcga ggaactgggg cgccgcatgg
     1501 cgggcagcca tatcgggaaa aacgagctgt gcctgatcgg ggacacgaag tatgatgctg
     1561 ccggagcaaa ggcgttcgga atccgctgta tcggtgtcag ctatggcttc ggtaccaggg
     1621 aagagctgct cgccgcaggt gcggaggcgg tcttcgaccg catcgaagag gtggagacat
     1681 acattgagaa tgacgcagcg ggagagggag aggcagagtg aaaacgacag gtgcttataa
     1741 aatctggaga atcatatatc cgatcggtat ctattatgtg gtgtcatcgc ttgcctactt
     1801 ttttctgacg ctgctgctcg gcgcggggga ggaatcctac atgctgcggc agatgctctg
     1861 cgcggctgcc acgattccgt tcgtctggat gcattatggg caggatcgcg ctgcgcggga
     1921 gactgtctat ggcaggcgcg gcatcagacc ggacgcaagg tttttctgca ctgtggcagg
     1981 ctcactgctt gcaggagcat ctcttggcat ggcggtcaac aacgtgattg ccatgacgcc
     2041 gctcattgag gcgtccgcgg gctttcaaag cgcgaatcag gcgttttttg ccggcggggt
     2101 tgtgtttgaa ctgctcggct cctgcctcgt gattccgctg gcggaggagc ttttgtaccg
     2161 tggtgtcgtg gagagaagac tatccctgct gtgcggcagt gcgccggcaa tcgtgctctc
     2221 ggcggttatt ttcggcgtga tgcactggaa cgtggtacag tttttgtat
//
LOCUS       DBMDQJ010000407         2268 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16593_length_2268_cov_1.390863, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000407 DBMDQJ010000000
VERSION     DBMDQJ010000407.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16593_length_2268_cov_1.390863"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            261..1754
                     /locus_tag="ACZO9R_08495"
     CDS             261..1754
                     /locus_tag="ACZO9R_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0913139.1"
                     /translation="MFGKKKEQSVETNGIVYRRAKTWRVALAGCSSGIGMCFYVLLGL
                     ASYVANEGYGIATAVVGIILTATRILDGVTDPIIAIIIDKMNTRFGKIRILMVAGWLI
                     ESLAVLIMYCWASGKGHGIVLFVLLYCLYVIGYTMCNVTAQIVPAMLTNDPKQRPMVG
                     VWSTAYNYLVPMILNIVITVILLPKYGNVYSVEMLAASCIVCVAVSGVGLLLCSIAVS
                     DIDKPENFVGVASKKKAEPVKVKDMWELVKSNRALQTFIVAASSDKIASQTASQAVVT
                     TMLFGIIIGNMQLGTILSVIGMLPSIIFAFIGAKYAGKHGNKEAMVTWTYVCITVAAV
                     LVVLFIFIDPTLIATTLPMMIVYVLLTLVLNGAKMCVTMSSNAMMADIIDYELDRSGK
                     FIPAAVTGTYSFIDKLVSSFSAAIATGLVALIGYRHTMPQPTDPSTPAIFWMTMSMYF
                     GLPLIGWVCTLCAMRETPLSKEKMEEVQKSIQEKKQAEVEKVIEEMK"
     gene            1785..>2268
                     /locus_tag="ACZO9R_08500"
     CDS             1785..>2268
                     /locus_tag="ACZO9R_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 2 galactose-binding
                     domain-containing protein"
                     /protein_id="HIR0913140.1"
                     /translation="MRNTIVLEKDWTFYKNPQSESGEAVTLPHTWNAVDGQDGGNDYY
                     RGTCKYVRHFAKPELEKGGRAYLEFNGAAMTADVVVNGTKLFHHEGGFSTFRVDVTEQ
                     LTEDNLLEVYVDNSDNTKVYPQKADFTFYGGLYRMVKLVTVPKVHFVMDYAGGNGTKV
                     T"
BASE COUNT          654 a          435 c          646 g          533 t
ORIGIN      
        1 agccgtattt ttcctgtgca tctcttgtaa tctgatgtta ctatataaga ttttataaaa
       61 ccgggagttt ataaaatcgt gaaaatgtta gaaaaatagc aatacatcat tttataacat
      121 gaattttagt acataacaac gaatttgtaa acacagaaag gaaaaagacc ggctagaata
      181 aagccataga aaaacagagg ccgcagatat agcagacata cggaagcggc aaaatatgga
      241 aaagtctgga gggtataaaa atgtttggaa agaaaaaaga acagtctgtg gagacaaacg
      301 gcattgtcta ccgccgtgca aagacatgga gggtagcgct tgcaggatgc agttctggga
      361 ttggaatgtg tttttatgtg ctgcttggac tggcaagcta tgtggcaaac gaagggtatg
      421 gcattgcaac agcggtggta ggcattatcc tgactgcaac aagaatcttg gacggcgtca
      481 cagatcccat cattgcgatc atcatcgata agatgaacac gcggttcgga aagatcagga
      541 ttctgatggt ggcaggatgg ctgatcgagt cattggcagt cctgatcatg tactgctggg
      601 cgagcggaaa gggacatgga atcgtgctgt tcgtgctgct ctactgcctg tatgtgatcg
      661 gctatacgat gtgcaacgtc acagcgcaga tcgttccggc gatgcttacg aacgacccga
      721 agcagcgccc gatggtaggc gtctggagta cagcttataa ctatctggtg ccgatgattc
      781 ttaacatcgt gattacggtg atattgctcc caaaatatgg aaatgtctat tcggtagaga
      841 tgcttgcagc atcctgcatc gtctgcgtgg cagtatcggg tgtgggatta cttctctgca
      901 gcatcgcggt atcggatatc gataaaccgg aaaattttgt gggcgtggcg agcaagaaga
      961 aggcggagcc ggtaaaagta aaagatatgt gggagctggt caaatccaac cgtgcgctgc
     1021 agacgttcat cgttgcggca agttcggata agatcgcgag ccagacggca agccaggcgg
     1081 ttgtgacgac gatgctgttc ggaattatta tcggcaatat gcagcttggg acaatcttaa
     1141 gtgtaatcgg tatgcttcct tcgatcatct tcgcgtttat cggggcgaaa tacgcaggaa
     1201 agcacggaaa taaagaggcg atggtgacgt ggacgtatgt gtgcatcacc gtggcggcgg
     1261 tgcttgtggt attgtttatt ttcattgatc cgaccctgat tgcgacgacg cttccaatga
     1321 tgattgtata tgtattattg accctggtgt taaatggtgc aaaaatgtgc gtgacgatgt
     1381 caagcaatgc gatgatggcg gacatcattg attacgagtt agaccgcagt ggtaaattca
     1441 ttccggcagc agtgacaggt acatacagct ttattgacaa gttagtatcc tcgttttctg
     1501 cagcgattgc aaccggtctg gtggcattga tcggctacag gcacacgatg ccacagccga
     1561 ccgacccgtc cacaccggca atcttctgga tgacgatgag catgtatttc ggactcccgc
     1621 tgattggctg ggtatgtacg ctctgcgcaa tgcgcgagac gccgctctcc aaagagaaga
     1681 tggaggaagt acagaagagc attcaggaga aaaaacaggc agaagtggaa aaagtgattg
     1741 aggaaatgaa atagagagaa gaaaagagag ggaaaacgga atagatgaga aatacaatag
     1801 tgctggaaaa agactggaca ttttataaaa atccacagag tgaaagcggg gaagcggtga
     1861 cgctcccgca cacctggaac gcggttgacg ggcaggacgg cggaaacgat tattatcgcg
     1921 gaacctgcaa atatgtgcgt cattttgcaa aaccggaact ggaaaaggga ggaagagcgt
     1981 atctggaatt taacggcgca gcgatgacgg cggatgttgt ggtcaacgga acgaagctgt
     2041 tccaccacga gggcggattt tctacattcc gggtagatgt gacggagcag ttgacagagg
     2101 acaatctgtt agaagtatat gtggataact ctgacaacac aaaggtgtat ccgcagaaag
     2161 ccgattttac tttttacggc gggctgtacc gcatggtaaa gctggtgacg gtgcctaaag
     2221 tgcattttgt gatggactat gcaggcggaa acggcacgaa agtgacgc
//
LOCUS       DBMDQJ010000408         2267 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16599_length_2267_cov_1.556325, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000408 DBMDQJ010000000
VERSION     DBMDQJ010000408.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16599_length_2267_cov_1.556325"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="ACZO9R_08505"
     CDS             <1..134
                     /locus_tag="ACZO9R_08505"
                     /inference="COORDINATES: protein motif:HMM:NF028184.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HIR0913141.1"
                     /translation="YRVETTGEVYCGDTLLHAGLNVPAMWGDFQSALIHLSEVPDEQ"
     gene            150..1181
                     /locus_tag="ACZO9R_08510"
     CDS             150..1181
                     /locus_tag="ACZO9R_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0913142.1"
                     /translation="MAKAVKLADIAGQLGVSTVTVSKALSGQKGVSEELREKIKTLAD
                     EMGYKQPSARRREHAVKSYNIGVLIAEHWLDKYDSFYLQMYQQVATRAVAKECFTLME
                     VVSAQMETACEMPKLVQEQKADGLIIIGRLTGEYLKFLNEKAKIPLLYMDFCDEKTDT
                     DAVISDSYYGAYRMTNYLFDMGHREIAYVGTLLATGSITDRYLGYTKSLMEHGVAVRG
                     DWTIADRDIHTGKIDMDRLLQLPKEMPTAFVCNCDLTASFLIRRLHEHGYRVPEDISV
                     VGYDNYLFPGLCDVAITTYEVDMKEMARQTVHTLIKKMAGEPYRQGICIVEGHMVTKD
                     SVKARKNMG"
     gene            complement(1178..2128)
                     /locus_tag="ACZO9R_08515"
     CDS             complement(1178..2128)
                     /locus_tag="ACZO9R_08515"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0913143.1"
                     /translation="MQKGPILMEPITIKARLNQAYHFSGAAGNGIRFSDLSDDPAFVF
                     REDTSAVHNRFVPAGGRISYASPVRLTRDALPYLLFLYVESGSGTLTTAGDAVQEPES
                     CQLAPGDLMLLPDQTAYRFSTTHTPFCYHCFYLSTEYFSEYLPFLCANDRIFHTNRTE
                     ADDIVWRILPCLSRELLSDEPVSSLHLTAMLSLVLSSLAASRRAASTPSLPAHVAHMK
                     EIFDTDYAHSHSLAALESSLGVSRYRLCHDFSDCIGVPPIQYLNRVRLKAARTLLRTT
                     TLTIREVSAAVGIENTTHFINLFKKDAGITPLQFRQSSLH"
BASE COUNT          612 a          471 c          713 g          471 t
ORIGIN      
        1 aatatcgtgt tgagacaacg ggggaagtgt actgcggaga taccctcctg catgcgggac
       61 tgaatgttcc ggcgatgtgg ggagatttcc agtcggcact gatacactta tccgaggtgc
      121 ctgatgaaca gtagttggag gatgaaaaaa tggcaaaagc ggtaaagctg gcagatattg
      181 cgggacagct tggcgtgagt acggttaccg tatcgaaggc gctgtccgga cagaaaggcg
      241 tcagcgagga actgcgggag aagataaaaa cgcttgccga tgagatggga tacaaacagc
      301 cgtccgccag gcggcgcgaa cacgcggtaa aaagctataa tatcggtgtg ctgattgccg
      361 agcactggct ggataagtat gattccttct atctgcagat gtatcagcag gtggcgacgc
      421 gcgcggtggc gaaggaatgt tttacgctga tggaagtggt cagcgcacag atggagactg
      481 cctgcgagat gccgaagctt gtgcaggagc agaaggcgga cggtctgatt atcatcggac
      541 gcctgacggg agaatatctg aagtttttga atgaaaaagc aaaaattccg cttctgtata
      601 tggatttctg tgacgagaag acagatacgg atgcggtgat ttcggacagc tattacggtg
      661 cttaccgcat gacgaattat ctgttcgaca tgggacacag ggagattgcc tatgtgggaa
      721 ccctgcttgc aacgggatcc atcacggacc gctatctggg gtatacgaaa tccctgatgg
      781 agcacggcgt tgcggtgagg ggggactgga cgatcgcaga ccgcgacatc catacgggca
      841 agattgatat ggaccggctg ttgcagctgc caaaggagat gccgacggct tttgtgtgta
      901 actgtgatct gacggcgagt tttctgatcc gcaggctgca tgagcacgga taccgtgtgc
      961 cggaggatat atcggttgtg ggatatgaca attatctttt cccgggactc tgtgatgtgg
     1021 cgatcaccac ttacgaggtg gatatgaagg agatggcgag acagacggtt cacacgctga
     1081 tcaagaaaat ggcaggcgaa ccgtaccgcc agggaatctg tatcgtcgag ggacatatgg
     1141 tcacaaagga cagcgtaaaa gccagaaaaa atatgggtta gtgcagggaa gactgccgga
     1201 attgcagagg tgtgattccg gcatcttttt tgaacagatt gataaaatgt gtcgtgtttt
     1261 caatccctac ggcggcgctg acctcacgga tggtgagggt tgtcgtgcgc agcagcgtgc
     1321 gtgcggcttt cagccggacc cggttcagat actggattgg cggaacaccg atgcagtctg
     1381 aaaaatcatg gcagagccgg tagcggctga ctccgagaga actctcgagc gcggcgagag
     1441 agtgggaatg tgcgtaatcg gtgtcgaaaa tctctttcat gtgtgccaca tgcgcgggaa
     1501 gggaaggcgt cgatgcagca cggcgggaag cggcgaggga ggacaggaca agcgagagca
     1561 tggcggtcag atgaagcgag gaaaccggtt catcggagag cagctcgcgg gagaggcagg
     1621 gcagaatacg ccatacgatg tcatccgcct cggtccggtt cgtatgaaaa atgcggtcgt
     1681 tcgcacacaa aaacggaaga tattccgaaa aatactctgt ggacagataa aaacagtggt
     1741 agcagaaagg ggtatgcgtc gtggaaaacc ggtatgcggt ctgatccgga agaagcatga
     1801 gatcccccgg tgcaagctgg caggattccg gctcctgcac agcgtcgccg gcagtcgtta
     1861 aagtgccgct tccggattcg acatagagaa agaggaggta cggcagcgca tcccgcgtca
     1921 gacggacggg ggacgcgtag ctgatgcgcc ctcctgcagg gacaaaacgg ttgtgtacag
     1981 ccgatgtgtc ctcgcggaaa acgaaagccg ggtcgtctga aagatcggaa aaacgaatgc
     2041 cgtttccggc tgccccggag aagtgataag cctgattcag gcgtgccttg atcgtgatgg
     2101 gttccataag aatcggtcct ttctgcataa ctatcctata ttatagcgga actattttgc
     2161 aatacaatag gtttcttgat tattaggtta aaaaatagca aaaattaagt aattaaaatt
     2221 aaattaagag gaaaaagaga gaaaaagaag aggctaaaaa tatgaaa
//
LOCUS       DBMDQJ010000409         2266 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16608_length_2266_cov_1.549192, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000409 DBMDQJ010000000
VERSION     DBMDQJ010000409.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16608_length_2266_cov_1.549192"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="ACZO9R_08520"
     CDS             complement(<1..254)
                     /locus_tag="ACZO9R_08520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR0913144.1"
                     /translation="MEKTVKKGRNSSIELLRIVCLVMIFWMHAADSGVANGIGAWIDI
                     AVAVIGNIGVSCFILISGYYGIRLDVKKMMHLECMLLFYS"
     gene            complement(259..1284)
                     /locus_tag="ACZO9R_08525"
     CDS             complement(259..1284)
                     /locus_tag="ACZO9R_08525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR0913145.1"
                     /translation="MKHWKYLDVARGIGLLLVIISHSCGLSPYLINYYIPLFFVVSGY
                     LYKPGRSYGENVVHKAKRLLVPYFGYSALLLVFYRAIGRTWQETGRSALGVLYSRYCL
                     FDTTTHQDNVFLFTVANGAMWYLTAFFVTSLVYHLVIDRCLKSRRFLIGCLVVLTVLT
                     MLLAELPVLLPWSLDLAFVGTIFMIAGTLLQRADFFDRDWNLWVIIGILVFYLSLSRA
                     NPGINMSVREYGVYQAFSVPFFILIGITGSMLCIWVGKAFQNCIVGTVLAYIGQNTIV
                     LLALHILGLEIFEMAAAKFINIGELTGAAFVLYHTVRVTASVCGCLLFGKTLDGIKRA
                     LHGKHRG"
     gene            complement(1293..>2266)
                     /locus_tag="ACZO9R_08530"
     CDS             complement(1293..>2266)
                     /locus_tag="ACZO9R_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HIR0913146.1"
                     /translation="GTDLDETKYAKQVTDYLGIRHTFLTIDDAVPEREFLRQLYCFEE
                     LWGNPQVPMMELYKKERELGTTVSLDGHAADELFAGYGFDVLKAYPDAKGKAEIDQIT
                     MAYLNQRSEMGYDPSSAEFKRQRNKLYRDYMTKYYAKKLLGRADVSSAYADHPNWKKL
                     DNLNKVLFVSTHETILPTLLRNYDRDSMASGVEIRMPFLDYRIVEFAFSIGWKSKLHG
                     GFSKSIVRDACADFMPHDIAYRRTKLGFNAPILDWMRGPYRQFFEDTVADPAFANCSL
                     IDDATGVRADLAKFLNGENATFHQAADIWNRIQPYLWERALIRQDWR"
BASE COUNT          584 a          672 c          496 g          514 t
ORIGIN      
        1 caggaataga acaaaagcat gcattccaga tgcatcattt ttttcacatc caggcggatt
       61 ccgtaatatc ccgaaatcag gataaaacag gatacaccga tatttccgat gaccgcaacc
      121 gcaatatcaa tccaggcgcc aatcccgttc gccacaccgc tgtccgcggc gtgcatccag
      181 aatatcataa caaggcacac aatccgcagc agttcgatac tcgagttacg cccctttttt
      241 actgtttttt ccatgtactt accctctgtg ctttccgtgc agtgcccgct tgattccatc
      301 aagagttttt ccaaacagca gacacccgca gacagacgcc gtcaccctca ccgtgtggta
      361 aaggacgaac gcggcgccgg tcagttcgcc gatattgata aactttgccg ctgccatctc
      421 aaaaatctca agcccgagaa tatgaagcgc cagaagaaca atcgtattct gcccgatata
      481 ggctaacacc gtacccacaa tgcagttctg gaatgctttt ccgacccaga tgcagagcat
      541 ggagcccgta ataccgatca gaataaaaaa cggaacactg aacgcctgat acacgccgta
      601 ctcgcgcacc gacatgttga tgcccggatt cgcccggctg agcgacagat aaaataccag
      661 tatgccaatg atcacccaca gattccagtc cctgtcaaag aaatctgctc tctgaagcag
      721 tgtacctgca atcataaaaa tcgttcccac aaacgcgaga tccagactcc acggaagcag
      781 taccggcagt tccgccaaaa gcatcgtgag cacggtaagc accaccagac agccgatcag
      841 aaaccggcgg ctctttaagc agcggtcgat caccagatgg tacaccagac ttgtcacaaa
      901 aaatgcggtc agataccaca tggcaccgtt tgccacggta aataaaaata cgttgtcctg
      961 atgtgtcgtc gtatcaaaaa ggcagtagcg tgagtacagc actccgagtg cagatcttcc
     1021 cgtctcctgc caggtacgtc cgattgcccg gtagaacacc agcagcaacg cgctgtagcc
     1081 aaaatacgga accagcaggc gcttcgcctt gtgcaccaca ttttccccat agcttctgcc
     1141 cggcttgtac agatagcccg acaccacaaa aaacagcggg atataataat tgatcaggta
     1201 cgggctcagc ccgcagctat gtgaaatgat gacgaggaga agtccgattc ccctcgccac
     1261 atccaggtat ttccagtgtt tcatccccac tcctatctcc aatcctgccg gatcagcgcg
     1321 cgttcccaca gatacggctg tatccggttc cagatatccg ccgcctgatg gaaggttgca
     1381 ttctcaccgt ttaggaactt tgcaagatcc gcacgcacgc ctgtggcatc gtcgattaag
     1441 ctgcagtttg caaacgccgg gtccgccacc gtatcctcaa aaaactggcg gtaaggtccg
     1501 cgcatccagt ccagaatcgg cgcattgaat cccagcttcg ttctgcggta ggcaatgtcg
     1561 tgcggcataa agtctgcgca ggcatcgcgg acgatggatt tggaaaatcc tccgtgcagc
     1621 ttggatttcc agccgatcga aaacgcgaac tccacaatcc tgtagtccag aaacggcatg
     1681 cggatctcaa cgccggacgc catactgtct ctgtcgtaat tgcgcaaaag cgtcggaaga
     1741 atcgtctcat gtgtggagac aaacagtacc ttattcagat tgtccagctt cttccagttc
     1801 ggatgatccg cgtaagcgct cgatacatcc gctcttccca gaagcttctt cgcataatac
     1861 ttcgtcatat agtcacggta cagcttattc cgctgccgct taaattctgc ggacgacggg
     1921 tcatatccca tctcactccg ctgatttaaa tatgccatcg taatctggtc gatctccgct
     1981 tttccctttg catccggata cgcttttaac acgtcgaatc cgtagccggc aaataattca
     2041 tctgccgcat ggccgtcgag agataccgtc gttccgagct ctctctcctt tttataaagc
     2101 tccatcatag gcacctgcgg attgccccag agttcctcaa agcagtacag ctgacgcaga
     2161 aattcccgct ccggcaccgc gtcgtcaatc gttaaaaacg tatgcctgat gccgagataa
     2221 tccgtcacct gctttgcgta ttttgtctca tccagatccg ttcccg
//
LOCUS       DBMDQJ010000410         2264 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16636_length_2264_cov_1.233472, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000410 DBMDQJ010000000
VERSION     DBMDQJ010000410.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16636_length_2264_cov_1.233472"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(172..1854)
                     /gene="ilvB"
                     /locus_tag="ACZO9R_08535"
     CDS             complement(172..1854)
                     /gene="ilvB"
                     /locus_tag="ACZO9R_08535"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR0913147.1"
                     /translation="MELTGAQIVIECLKEQGVDTVFGYPGGAILNVYDELYKHQEIRH
                     ILTSHEQGASHAADGYARSTGKVGVCFATSGPGATNLVTGIATAYMDSVPIVAITCNV
                     NVPLLGKDSFQEIDIAGITTPITKHNFIVKDVTKLADTIRRAFRIAQKGRPGPVLVDI
                     PKDITAAKTEYEFCKAEPIARVTDTIREEDLAKALAMIKAAEKPYIFVGGGAVISGAS
                     AELKEFVHKVDAPVCDSLMGKGAFDGTNDLYTGMLGMHGTKTSNLGVSECDLLIAVGV
                     RFSDRVLGNAKKFAKQAKILQFDVDAAEINKNIRVDAAVIGDLKEILKRINAELPQLG
                     HEAWIAHILDYKVKYPLTYQEPGLTGPYLVEEIYRQTNGDAIIVTEVGQHQMWAAQYY
                     KYKNPRTFLSSGGLGTMGYGLGAAIGAQVANPGKQVINIAGDGCFRMNMNEIATAVRQ
                     KLPLIEVIVNNHVLGMVRQWQDLFYEKRYSATVLDDGVDYVKLAEAMGATGYRATTRE
                     TFNEALKAALAAKTPVVIDCVIGCDDKVWPMVAPGADISTAFTGEDLAAAQS"
     gene            complement(1878..>2264)
                     /locus_tag="ACZO9R_08540"
     CDS             complement(1878..>2264)
                     /locus_tag="ACZO9R_08540"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742718.1"
                     /note="catalyzes the dehydration of
                     2,3-dihydroxy-3-methylbutanoate to 3-methyl-2-oxobutanoate
                     in valine and isoleucine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR0913148.1"
                     /translation="RYEGPKGGPGMREMLNPTSAIAGMGLGSSVALIIDGRFSGASRG
                     ASIGHVSPEAAVGGPIALVEEGDLIKINIPELKLELAVPDEELSARKAKWQPREPKVT
                     TGYLKRYASLVTSGNRGAILALPEEQ"
BASE COUNT          460 a          709 c          493 g          602 t
ORIGIN      
        1 agcatctcat ctgctgcttc ttttaagacc tcttccggca gcactgccgg acctgctgaa
       61 aaattgtaca ctctgctcat tttctaattc ctttcctttc ctttctcttt tattacggca
      121 gttccctacg ctgccgcctc catgcaccct gtaacaaaac atatacacat gttatgactg
      181 cgctgcggct aaatcctccc cggtaaatgc ggtactgatg tccgcccccg gagcaaccat
      241 cggccacacc ttgtcatcac agccgatcac acaatcgatc actaccggcg tctttgcagc
      301 aagcgctgct tttaacgcct cattaaatgt ctcgcgggtt gtggcgcggt accctgtcgc
      361 ccccatcgcc tccgcaagct tcacatagtc gacgccgtcg tctaaaaccg tcgccgaata
      421 acgtttttcg taaaacagat cctgccactg ccgcaccatg ccgagcacat gattgttcac
      481 aatcacttcg atcaacggca gcttctgccg gactgcggta gcaatctcgt tcatattcat
      541 gcgaaaacat ccgtcccccg cgatattgat cacctgtttt cccggatttg caacctgtgc
      601 accgatcgca gcgcccagtc cgtatcccat ggtgccaagt ccgccggatg ataaaaacgt
      661 gcgcggattt ttatatttat aatactgtgc ggcccacatc tggtgctgtc ctacttctgt
      721 cacaatgatg gcgtcaccgt ttgtctggcg gtagatctcc tccaccagat aaggtccggt
      781 cagtcccggc tcctggtatg tgagcggata ttttacttta tagtccagaa tatgcgctat
      841 ccacgcttca tgccccagct gcggcagttc cgcgttgatc cgctttaaaa tttctttcag
      901 gtctccgatc acagccgcat cgacacggat atttttattg atctccgctg catccacatc
      961 aaactgtaag atcttcgcct gctttgcaaa cttcttggca ttgccgagca cacggtcgga
     1021 aaagcgcacg ccgacggcga tcagcagatc acactcgctc acaccgaggt tggacgtctt
     1081 ggttccgtgc atgccgagca tccccgtgta gagatcgttc gtcccgtcaa aggctccctt
     1141 tcccatcagg gaatcgcaca ccggtgcgtc cactttatgt acaaattctt ttaattccgc
     1201 gctcgccccg gagatcacag caccaccgcc cacaaatatg tacggtttct ctgccgcttt
     1261 tatcatcgca agcgccttcg caagatcctc ttctcgtatc gtgtccgtca cacgcgcgat
     1321 cggctctgcc ttgcagaatt catactccgt ctttgccgcc gtaatatcct tcgggatatc
     1381 gacgagcacc gggcccggac gccccttctg tgcgatccgg aatgctctgc ggatcgtgtc
     1441 cgcaagcttt gtgacatcct ttacgataaa attgtgcttt gtgatcggcg tcgtgatgcc
     1501 tgcaatatcg atctcctgaa aactgtcttt gccgagaagc ggcacattga cgttgcatgt
     1561 gatcgctacg atcggtacgg aatccatgta tgccgtggca attcccgtca caagattcgt
     1621 cgctcccggt ccggacgttg cgaaacagac tcctactttt ccggtacttc tcgcataacc
     1681 gtccgccgca tgggaagctc cctgctcatg tgaagttaaa atatgccgta tctcctgatg
     1741 cttatataat tcatcgtata cattcagtat ggctccgccc gggtatccga atacggtgtc
     1801 cacaccctgt tctttcagac attcgatgac aatctgtgct cctgtcagct ccatgtcgta
     1861 taccttcctt ttctatccta ctgttcttcc gggagcgcca gtattgctcc gcggttgccc
     1921 gaggtcacga gcgatgcgta ccgctttaag tatccggtcg tcaccttcgg ctctctcggc
     1981 tgccatttcg cctttctggc ggaaagttcc tcgtccggta cggcgagttc cagctttaac
     2041 tccggaatat tgatcttaat aagatctccc tcttccacca gtgcaatcgg tcccccgacc
     2101 gctgcttccg gcgatacatg gccgatcgac gcgccgcggc ttgcaccgga aaaacgtccg
     2161 tcgataatca gggcaacgga actgccaagc cccataccgg caatcgcaga agtcggattt
     2221 aacatttctc tcataccagg tccgcccttc ggtccctcat agcg
//
LOCUS       DBMDQJ010000411         2247 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16785_length_2247_cov_1.203635, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000411 DBMDQJ010000000
VERSION     DBMDQJ010000411.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16785_length_2247_cov_1.203635"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..2191)
                     /locus_tag="ACZO9R_08545"
     CDS             complement(<1..2191)
                     /locus_tag="ACZO9R_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913149.1"
                     /translation="MNSKRILSLVLAMVLAVSLCLPGTTAYAIEPEDDDLESEQVIPT
                     EPALCSYTSNENDRHADNVTLSEAVGVLNENGGGTITVTRSGLASNGSSAILNLEVEQ
                     DITIVAEENRPVTVTLTEPMFMMDCQGNAYGKLTLGQEGMTSGLLTFDGVQKALCFAR
                     SNWRNTKPFTRTRKVEINDGIVMKNFAFSVMGMQGFAGLDVTMHGGEILENTPEASWS
                     LGAYTYSSTIVTCMNFYMDGGSIHDNTANDSIINVIIDTGFEDVQPVKICGDAKIYKN
                     SHDPEGTTKMSSVVSASHDVILSDDAKIYGCEGTADLVSCYSNDITMKDNASVYDCTS
                     TGQSQGLSAIHGVNVTMEDNAAVYNCTSEIAGAVSGNRSVTMSGNAAIRNCKTLVADK
                     DYPANGVAVYAGISPYVKDNEVSFHMQDNAVISNCSGPAAAGGVYVKNGKAWMSDQAK
                     IEYCSTPQLSDYAHIYIDSQYMAGGVYILGGSLTMSGQSNIDHCSTYGSAGGVYMAYA
                     SSEIVMEDQASITNCTNLNTTVDTEGTDNYRVGGLSAEKTTVTIGQNAKITGCKGIIG
                     GIGCKTGFLDTKGTSSVSIAGEVSGNTGVVGGGVCGLRSVQLTLENTAVIKNNTATIA
                     GGGIALLSYAGEVAPASELTLNGASIIGNNAPLGGGVFVAGKADIPEAPVFYSSAYAE
                     KASISSVTITGGTITGNTAGNPDGGAASEFHGGGLFLGWNTKTNISGA"
BASE COUNT          534 a          594 c          477 g          642 t
ORIGIN      
        1 cggctccgga aatgtttgtt tttgtattcc agcccaggaa caggccgccg ccatgaaact
       61 ccgaggcagc accaccatca ggatttccgg cggtattccc cgtaatcgtt ccacctgtta
      121 tggttacgct gctgatactt gctttctcag cgtatgcact tgaataaaag acaggtgctt
      181 caggaatatc cgcttttccg gccacaaaga cgccgccgcc caaaggcgcg ttatttccaa
      241 tgatactggc accgttcaat gtcagttcag aggcaggggc aacttcaccg gcataactga
      301 gcaacgcgat accgccccca gcaattgttg ccgtattgtt tttaataaca gctgtatttt
      361 ccagcgtaag ctgaacgctt ctcaggccac aaacaccgcc gccgaccaca ccggtatttc
      421 cgctgacttc gccggcaatt gaaacggaac tggttccctt cgtatcgagg aagccggtct
      481 tgcagccaat gccgcctatt atacccttgc atcctgtaat tttagcgttt tgtccaatcg
      541 taacggttgt tttttcggcg cttaatccgc ccactctata attatcggtg ccttctgtat
      601 ctaccgtagt attaagattc gtgcaatttg tgatggacgc ctgatcttcc atcacaattt
      661 cactgcttgc atatgccatg tagacgccgc cggccgaacc gtatgtggag cagtggtcaa
      721 tattggactg tccgctcatg gtaagactgc cgccaagtat atatacgccg ccggccatgt
      781 actgcgaatc aatgtatata tgtgcgtaat cagatagctg gggagtagag caatattcaa
      841 tctttgcctg atcgctcatc catgcttttc cgtttttcac atagacaccg cccgctgccg
      901 ccgggccgct gcaatttgaa atcacagcgt tgtcctgcat gtgaaagctg acttcattat
      961 cctttacata aggagatatt ccggcataca cagctacacc gtttgccggg tagtctttgt
     1021 cagcgaccag agttttgcaa ttacggattg cggcattgcc actcatcgta actgagcggt
     1081 tgccgcttac tgcaccggcg atttcagacg tgcagttata aactgctgcg ttgtcctcca
     1141 tcgtgacgtt tactccgtgg attgccgaaa gcccctgtga ctgtcccgtg ctggtgcagt
     1201 cgtaaacgga ggcattgtcc ttcatagtga tatcgttgct ataacaactc accaaatctg
     1261 cggtgccttc acagccatat attttggcat catcagacag aattacatca tggctggcac
     1321 ttacaaccga actcattttc gtagtgcctt cagggtcatg gctgtttttg taaattttag
     1381 cgtcaccgca aatctttaca ggctgtacat cctcaaagcc ggtatctata atgacgttta
     1441 tgatgctgtc atttgccgtg ttatcatgga tgcttccgcc gtccatatag aaattcatgc
     1501 aggtcacaat ggtagatgaa tatgtgtagg cgccaagtga ccagcttgct tccggagtat
     1561 tctccagaat ttcgccgccg tgcatggtca catcaagccc cgcaaagcct tgcatcccca
     1621 tcacggagaa ggcaaaattc ttcatcacaa tgccatcatt gatttcaact tttctcgtgc
     1681 gtgtaaaagg cttagtattt ctccagttac tccgtgcaaa gcatagcgct ttctgcacac
     1741 catcaaacgt caaaagaccg gaggtcattc cttcctgtcc aagcgtcagt tttccgtaag
     1801 catttccctg acagtccatc ataaacattg gctccgtcag ggtaacagta acaggacgat
     1861 tttcctctgc aacaatcgta atatcctgct caacttcgag atttaaaata gcgcttgaac
     1921 catttgacgc caaaccgctt ctggtaactg taatcgtacc gccgccgttc tcatttaata
     1981 cacctactgc ctcagacaag gtgacattat cagcgtgccg gtcgttttca ttgctcgtat
     2041 aagaacataa tgcaggttcg gtaggtatta cttgctctga ttccagatca tcgtcctccg
     2101 gctctatggc atatgccgtt gtgccgggaa gacataatga tactgctaag accatcgcca
     2161 atacaagcga tagtattctt tttgagttca taatgtttct tcctcttttc gtttttagac
     2221 gtatcgcaat atatataaat tacccat
//
LOCUS       DBMDQJ010000412         2235 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16900_length_2235_cov_1.177132, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000412 DBMDQJ010000000
VERSION     DBMDQJ010000412.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16900_length_2235_cov_1.177132"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            96..455
                     /locus_tag="ACZO9R_08550"
                     /pseudo
     CDS             96..455
                     /locus_tag="ACZO9R_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080364.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog.; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
     gene            591..663
                     /locus_tag="ACZO9R_08555"
     tRNA            591..663
                     /locus_tag="ACZO9R_08555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:624..626,aa:Val,seq:tac)
     gene            682..755
                     /locus_tag="ACZO9R_08560"
     tRNA            682..755
                     /locus_tag="ACZO9R_08560"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:716..718,aa:Met,seq:cat)
     gene            840..1388
                     /gene="ispF"
                     /locus_tag="ACZO9R_08565"
     CDS             840..1388
                     /gene="ispF"
                     /locus_tag="ACZO9R_08565"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015549329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR0913150.1"
                     /translation="MRVGMGYDVHKLVEGRDLIIGGVKIPHTLGLLGHSDADVLLHAI
                     MDALLGAAGLGDIGKHFPDTDPQYKGISSMKLLSHVAELIRERGYVVENIDATIIAQK
                     PKMRPHIPQMEENIAGALGISTDQVNVKATTEEGLGFTGTEEGISSQAICALSSFYEG
                     SFTVADSAAGCAGCGGCGRSVQ"
     misc_binding    1511..1751
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1822..>2235
                     /gene="epsC"
                     /locus_tag="ACZO9R_08570"
     CDS             1822..>2235
                     /gene="epsC"
                     /locus_tag="ACZO9R_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HIR0913151.1"
                     /translation="MGILRYMKEEFQVIKERDPAIKSPMEVSLYPTFHVMLSYRRAHK
                     LYLKGHYFWARFISQRAARKTGIEIHPGATIGKGFFIDHGSGVIIGETSIIGDNVTLY
                     QGVTLGGTGKETGKRHPTIGNNVMISAGAQIIGSFT"
BASE COUNT          601 a          429 c          686 g          519 t
ORIGIN      
        1 actgttaaaa tgtcatacga atgcttttga ttttggggga ttattatgac atcggaaaaa
       61 ataattgata tcttaaaaaa cggtaaaatt catctgtcgt ggcgaccgtg gagggtgata
      121 tccatgacat cgggaagaat cttgtggtgc tgatgctgaa aaattacggg tatcatgtga
      181 ttgatctggg aaaagatgtc ccggcagatc tgattgtaga taccgcgatg aaggaaaatg
      241 ccagggtgat agggctctcc gcgctgatga cgacgacgat gatgcgcatg aaggacgtgg
      301 tggagcttgt caaagagcgc ggatgtacgg cgaaggttgt catcggaggt gcggcgatca
      361 cggaaagctt tgcggaggag atcggagcag acggctattc caaggatgcg gcggaatgtg
      421 tgaagctggt ggatcgtctg ttggaggaag agtagaaggc gaaaagcggt cttttccgcc
      481 ttcaaactgc cccgggaaaa tagatgcaaa aaaatagaaa aaaagtgttg actttttgca
      541 atacggatag tatactaatc aagtcggttg cggtagcgac cgatgaatat gggatcttag
      601 ctcagctggg agagcatctg ccttacaagc agagggtcat aggttcgagc cctataggtc
      661 ccatagatat caatataata tggcggaata gctcagttgg ctagagcaca cggttcatac
      721 ccgtggtgtc gagagttcaa atctcccttc cgctactaga gagtcctcgg gaatacaagc
      781 agttgtgtgt ccggggattt ttgttctttc ataggaaaag gcttgaaagt gagggtatca
      841 tgagagtagg aatgggttac gacgtgcata aactggttga gggcagagat ctgatcatcg
      901 gcggtgtcaa gatcccgcat acgcttggtc tgctcggaca ttcggatgcg gatgtgctgc
      961 ttcatgcgat tatggatgcg cttttgggcg cggcgggatt aggcgacatt ggaaagcatt
     1021 ttccggacac tgatccgcag tataagggaa tttccagtat gaagctgctc tcccatgtgg
     1081 cggagctgat ccgggagagg ggttacgtgg tggagaacat tgatgcgacg atcattgcgc
     1141 agaagccgaa gatgcgcccg catatcccgc agatggagga aaacatcgcg ggagcgctcg
     1201 gcatcagtac ggatcaggtc aatgtcaagg cgaccacgga ggagggactt ggcttcaccg
     1261 gaacggagga agggatttcc tcacaggcga tctgcgcgct ttccagtttt tatgagggaa
     1321 gttttactgt ggcggactct gcggcaggct gcgcgggctg cggcggctgc ggacgaagcg
     1381 tacaatgagc taagaagaat tcaggcggct gcagacgaaa catgcagtga aaaaagattg
     1441 acaaaaacac aggaagtgca taaactatag taggcaggag aggtatcttc tgccgataaa
     1501 aagatgaatt ggaaaagcga agatcggaaa gagtaaggat tcggacattc gtcagagaga
     1561 gactgtcacc ggctgaaagc agtcttggaa gagagagtcc ccaagtgcct ccgtgagctg
     1621 atccggcgag agtcatctgc accgcaggat gtagggatgc cgtagccggg tacgtctggc
     1681 atacgttaca tgcgtcaagt gaaatcatat gattttagta gagtggaacc gcggatcatt
     1741 tcgtctctag gagacggatg atcctttttt ttgtaatagg aaactttgtt ctggtttgta
     1801 tgtggaaagg aatgggaaga gatgggtatc ctaaggtata tgaaggaaga gtttcaggta
     1861 atcaaggaac gcgatccggc gatcaaaagt ccgatggaag tttcgcttta tccgacattt
     1921 catgttatgc taagctacag aagggcgcat aagctttatc tcaaagggca ttatttctgg
     1981 gcgcggttta tttcgcagcg cgcagccagg aaaacaggca ttgagatcca tccgggagcg
     2041 acgatcggca agggattttt tatcgatcac ggaagcggcg tcatcatcgg cgagacgtcg
     2101 atcatcggag ataacgtgac gctgtatcag ggcgtgacac ttggcggtac cggcaaggag
     2161 accggaaagc gtcatccgac gatcgggaac aacgtcatga tcagtgctgg ggcgcagatt
     2221 atcggttctt ttacg
//
LOCUS       DBMDQJ010000413         2231 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16933_length_2231_cov_1.827700, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000413 DBMDQJ010000000
VERSION     DBMDQJ010000413.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16933_length_2231_cov_1.827700"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="ACZO9R_08575"
     CDS             complement(<1..855)
                     /locus_tag="ACZO9R_08575"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 53 protein"
                     /protein_id="HIR0913152.1"
                     /translation="MKFVKGMDLSTLLELERCGAKYYDNGEERDLLAIMKSYDVDTIR
                     IRLWNDPWSETGESYGAGENDLKTSLEIAKRVTAAGFGVLLNFHYSDFWADPGKQIKP
                     KAWADYGVKELEQAVYDYTLESMRTFLDAGVNITMVQVGNELSNGLLWPEGKVPNYDN
                     IATFVNAGIRAVRKADATIPVMIHLDNGGNNALYREWFDNFTKRGEDFEIIGLSYYPF
                     WHGSLQMLNDNMNDIAERYGKDLVIAEVSMGYTMEDYKNYEKLSDEERKGYATRPALV
                     EKIEYPMTK"
     gene            complement(912..>2231)
                     /locus_tag="ACZO9R_08580"
     CDS             complement(912..>2231)
                     /locus_tag="ACZO9R_08580"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit family protein"
                     /protein_id="HIR0913153.1"
                     /translation="PKMQEVKFNYQNISVSFTEDGFTVKNKNLFTDTAEYDCILLLEK
                     NGVAAGRRKIALSVAPLSEKTFVLEELEGCMALADELEHAGKEAGAEYALTVSFVLRG
                     ELLWAKPGHEIAFGQKIFAAKSPANGGTIGDAAAEYEGKCVTNRKAGLQIIRGKWNLG
                     VKGENFDVLFSYNSCGLVSYRYMGKELIEKIPMPNFWRAPIDNDCGSRMQGRMAQWKI
                     ASMYAGMSSKGMFDYEEPVVREAENSVSITYTYLLPTTPQAKCRVTYTVTPDAYVQTE
                     LTYDPVEELGDMPEFGMLFKLNADYDRLEWYGLGPQETYADRRCGAKLGVYKNRVADN
                     MAQYLVPQECGNKVGVRYAKVTDERGRGMLFTGDELSFSALPYTPHELENAMHAYELP
                     EVHYTVVRVALAQMGIGGDDSWGAPVHPEYHIDVTKPLTLRFGFRGI"
BASE COUNT          466 a          694 c          478 g          593 t
ORIGIN      
        1 ctttgtcatc ggatattcga tcttttccac aagcgccggt ctggtcgcat atcccttgcg
       61 ctcttcatcg gaaagcttct cgtaattctt gtagtcttcc atcgtatagc ccatggaaac
      121 ctcggcaatc accagatcct tgccgtagcg ctctgcaatg tcattcatgt tgtcgtttaa
      181 catctgcagt gatccgtgcc agaacggata ataggaaagt ccgataatct cgaaatcctc
      241 cccccgcttt gtaaaattgt cgaaccactc gcggtacagc gcattgtttc cgccgttatc
      301 cagatgaatc atcaccggaa tcgtggcatc cgctttccgc accgcacgaa ttcccgcatt
      361 gacgaatgtc gcaatattgt catagttcgg gacttttccc tccggccaga gcagaccgtt
      421 ggaaagttca tttcccacct gcaccatggt aatattgacg ccggcatcca ggaatgtccg
      481 catggattcc agcgtgtagt catagaccgc ctgctccagc tccttcacac cgtaatctgc
      541 ccatgccttc ggcttgatct gtttgcccgg gtctgcccag aaatcgctat agtgaaaatt
      601 gagaagcacg ccgaaccctg ccgccgtcac acgctttgca atctcaagag aagttttcaa
      661 atcattctcc cctgcgccgt aagattcgcc ggtctccgac cacggatcat tccacagacg
      721 gatacggatg gtatcaacgt catagctctt catgatcgca aggagatccc gctcctcccc
      781 gttatcgtaa tactttgcac cacaccgctc cagttcgagc agtgtggaca gatccattcc
      841 ttttacaaac ttcatatgcc tcttcgcggg cacagatctc attccatgcc cgctctctcc
      901 tttctgtttt tttatattcc acggaaaccg aaccgcagtg ttaacggctt tgtcacatca
      961 atgtgatatt ccggatgaac cggagctccc cagctgtcgt ctccgccgat gcccatctgt
     1021 gccagtgcca cacggacaac cgtgtaatgt acctcaggca attcatacgc atgcattgca
     1081 ttttccagtt catgcggtgt ataaggcagt gcggaaaatg acagttcgtc tccggtaaag
     1141 agcatgcctc ttcctctctc atccgtcacc ttcgcgtagc gcacgcctac tttgttgccg
     1201 cactcctgcg gtaccagata ctgcgccata ttatccgcca cgcggttctt atatacaccg
     1261 agcttcgcgc cgcatctgcg gtccgcatac gtctcctgcg gtccaagtcc gtaccactcc
     1321 agtctgtcgt aatccgcatt cagcttaaaa agcattccaa actccggcat atcgcccagc
     1381 tcctccaccg ggtcataggt cagctcggtc tgcacatacg catccggcgt cacggtgtag
     1441 gtcacgcggc attttgcctg cggcgtggtc ggaagcaggt aggtatatgt gatggagacg
     1501 ctgttctccg cctctctcac aaccggttcc tcataatcga acatgccctt gctgctcatg
     1561 ccggcataca tactcgcgat cttccactgc gccatccgcc cctgcatgcg gcttccgcag
     1621 tcattgtcaa tcggcgcacg ccagaaattc ggcatcggta tcttctcaat cagctctttt
     1681 cccatgtagc ggtaggacac cagtccgcag ctgttgtagg aaaacagtac gtcaaaattc
     1741 tctcccttca cccccagatt ccactttccg cggatgatct gaagtcccgc cttacggttc
     1801 gtcacgcact ttccttcata ttctgccgcg gcatcgccga ttgtcccacc atttgcaggt
     1861 gattttgccg caaaaatctt ctgtccgaat gcgatctcat gccccggctt tgcccagagc
     1921 agttctccgc gcagtacaaa cgatacggtc agtgcgtatt ccgccccggc ttcctttccc
     1981 gcatgttcca gttcatcggc aagagccatg cagccctcca gttcttccag aacaaacgtc
     2041 ttttccgaga gcggtgccac agacagcgct atttttctgc ggcctgccgc cacgccgttc
     2101 ttctccagca gaaggatgca gtcatactct gccgtatcgg taaacaggtt tttatttttt
     2161 accgtaaaac catcttcggt aaaggatacg ctgatgttct ggtaattgaa tttgacttcc
     2221 tgcatttttg g
//
LOCUS       DBMDQJ010000414         2228 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16951_length_2228_cov_1.272685, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000414 DBMDQJ010000000
VERSION     DBMDQJ010000414.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16951_length_2228_cov_1.272685"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            134..1069
                     /locus_tag="ACZO9R_08585"
     CDS             134..1069
                     /locus_tag="ACZO9R_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HIR0913154.1"
                     /translation="MILNTGSRTDIPAYYSDWFYERVRAGEVLVRNPYYPTQITRYRL
                     DPAVVDALVFCTKNPLPMLERLSMLDAFTMFWFVTITPYGREIEPHVPDKNLIAEAFC
                     RLSESVGRERVSFRYDPVFLNETYSVQHHVEKFGELAEKLSGYTGQCVVSFIDLYEKT
                     RRNFPDARSVGKEEQERLIAAFSEIAVKQHLQIHLCCENRSLVRQNVDADGCLSQSVL
                     ERAIGCRLKVPQQKRARDGCPCLLGADIGAYNTCGHECLYCYANYDSRTVRENRLQHD
                     PRSPLLIGHVQSTDIVKDAIQRSWKDPQMSIFDLY"
     gene            1102..2100
                     /locus_tag="ACZO9R_08590"
     CDS             1102..2100
                     /locus_tag="ACZO9R_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR0913155.1"
                     /translation="MKKELNWAVLGCGVIANEMAQALEHMGKHLYAVGNRTYEKAVAF
                     AEKYGVQKVYDDFHEMFHDPDVDVIYITTPHNTHIEFSREALAGGKHVLCEKSVMLNS
                     RELAEAIALAEEHHVVFAEAMTIWHMPLYKKLWQIVETGDGEIPALGKVQFIQLNFGS
                     YKEYNMKNRFFNMNLAGGALLDIGVYALSIARSFMSSQPDQVLSQVKYAPTGADEQEG
                     ILLMNPDGQMAALSLSLHSKQPKRAVISCEKGYIEIMEYPRADRAIVVEAETGKVHEI
                     TAGATADALLYEMTDMEDAILNGNTGVMRLKDTRDVMELMTGLRKEWNMKYPGEEW"
BASE COUNT          632 a          451 c          632 g          513 t
ORIGIN      
        1 ctttgccggc aggagaagtc aaggtttaca taaaatcaat ttcgggaagg ataccatccg
       61 tccggggagc aaaattcaga agtgaaaaac aggaaaaaat agggtataat ctggatatgc
      121 ggaggtgaag ggtatgatac tgaatacggg aagcaggaca gacataccgg cgtattacag
      181 cgactggttc tatgagaggg tccgcgcggg agaagttctg gtgcgcaatc cgtattatcc
      241 cacgcagata acaagatacc ggctcgatcc tgcggtggtg gatgcacttg tattctgtac
      301 gaaaaatccg ctgcccatgc tggagcgcct ttctatgctg gatgcgttta ccatgttctg
      361 gtttgtgacg atcacacctt acggcaggga gatagaacca catgtgccgg ataagaacct
      421 catcgcggag gcattttgca gattgtcgga gagcgtggga agagaacgtg tcagtttccg
      481 ttatgatccc gtctttttaa atgagaccta cagcgtgcag catcatgtgg aaaagttcgg
      541 ggagcttgca gagaagctgt ccggatatac cgggcagtgt gtggtaagtt ttattgattt
      601 atatgaaaaa acaagacgga actttccgga tgcaagaagc gtcggaaagg aggagcagga
      661 gcgtctgatt gcggcgtttt ccgagatagc agtaaagcag catcttcaga ttcatctgtg
      721 ctgtgagaac cgcagtcttg tccgacagaa tgtggatgcg gacggctgtt tatctcagtc
      781 tgtattggaa cgtgctattg gttgtagatt gaaggtgccg cagcagaaac gggcaagaga
      841 cgggtgtccc tgcctgctcg gcgcagacat cggtgcttat aacacctgcg gacatgaatg
      901 tctgtactgc tatgccaact atgacagccg caccgtccgg gagaaccggc tgcagcatga
      961 tcctcgctcg ccgcttctga tcgggcatgt gcagagcaca gacattgtaa aagatgcaat
     1021 ccagcgttcc tggaaagatc cacagatgag tatttttgat ttgtattgat aaaaaagtaa
     1081 aatcatgcag gaggtaccac aatgaaaaaa gagttgaact gggcagtatt gggatgcggt
     1141 gtcatcgcaa atgaaatggc acaggctcta gagcatatgg gaaagcatct ctacgcggtc
     1201 ggcaaccgca catacgagaa ggcagtcgca tttgcggaga agtacggtgt gcagaaggtg
     1261 tacgatgatt ttcatgaaat gttccatgat cccgacgtag atgtcattta cattaccacg
     1321 ccgcacaata cgcatatcga attttcccgt gaggcacttg caggcgggaa gcatgtgctc
     1381 tgtgaaaaat cagtcatgtt gaacagccgt gaacttgcgg aggcgattgc gcttgcagag
     1441 gagcaccacg ttgtgtttgc ggaggcgatg acgatctggc acatgccctt atataagaag
     1501 ctctggcaga ttgttgagac gggggatgga gagattccgg cactcgggaa ggtgcagttt
     1561 attcagctca atttcggcag ttacaaggaa tataatatga agaaccggtt tttcaatatg
     1621 aatctggcgg gcggagcact cttagacatc ggtgtctatg cactttccat tgcaagaagc
     1681 tttatgtcaa gccagcctga tcaggtactt tcccaggtaa aatacgcacc gaccggcgcg
     1741 gatgaacagg agggaattct tctgatgaat ccggacggac agatggcggc gctgtcgctg
     1801 tccctacact ccaaacagcc aaagcgcgct gtaatcagct gtgaaaaagg atatatagag
     1861 atcatggaat atccgcgcgc cgaccgtgct atcgttgtgg aagcagagac cggaaaggtg
     1921 catgagatca cggcaggtgc aacggcggat gcattattgt atgaaatgac agacatggaa
     1981 gatgctatct tgaacggaaa taccggggta atgcgcttaa aagatacccg tgacgtcatg
     2041 gaactcatga caggcctgcg caaagagtgg aacatgaaat acccaggaga agaatggtaa
     2101 ttgtgaaaaa aatatcaatt agggatagac agcgttcaga aaaagaatta taataggatc
     2161 tgcagaggat ggagatatca tcctgataaa agaatataga agtctttgtg gcagggtgga
     2221 agtcccta
//
LOCUS       DBMDQJ010000415         2223 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_16988_length_2223_cov_1.887842, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000415 DBMDQJ010000000
VERSION     DBMDQJ010000415.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16988_length_2223_cov_1.887842"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="ACZO9R_08595"
     CDS             complement(<1..313)
                     /locus_tag="ACZO9R_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913156.1"
                     /translation="MIVCGIALGLAVLGWAVSRKWGRKDWQKGAAVIAAAACLGLLSG
                     YMELQTDEDPEKGLLRRENGEGDYEADLRMYVDGAKKAKAYKVHVPEQELTKQEEQAY
                     LR"
     gene            complement(345..1082)
                     /locus_tag="ACZO9R_08600"
     CDS             complement(345..1082)
                     /locus_tag="ACZO9R_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR0913157.1"
                     /translation="MDYRKYRAGFVEKLACAAVGAGAAGMAAWLFYRSVWGMLLFPAA
                     YLVCVKKYCTLQKEKRKEQLLMEFKDAMQSASAALLAGYSVENAWRETEKELLELHGE
                     KGFMAAEVRWMNEGVRMNEPLERLLLSFAARSGCEEIESFAEVFAFAKRSGGDFVHIM
                     QTTVQKLAGRIEVEREVATVLAGKRLEGRIMEIMPLAILAYLQVASGEFLDALYGSAF
                     GVAVMSIAFCCYLAAIKWSEHILNIQI"
     gene            complement(1072..>2223)
                     /locus_tag="ACZO9R_08605"
     CDS             complement(1072..>2223)
                     /locus_tag="ACZO9R_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HIR0913158.1"
                     /translation="QLIEAQVRDFARERMLTLGEREAVVQTLFYSLRKLDVLQELLED
                     PTITEIMVNGAEHIFYEKAGRLYKAEYGFSSKEKLNDVIQQMAGYSNRMVNEASPIVD
                     TRLADGSRVNIVLEPVAIDGSVVTIRKFPENPIGMEDLIRMGSISQEAADLLKILVRC
                     GYNIFISGGTGSGKTTFLNALSQYIPREERIITVEDSAELQLLDKPNLVRLETRNANT
                     EGVTPITIRDLIRTALRMRPERIIVGECRGAEALDVLQAMNTGHDGSLSTGHANSCKD
                     MLSRLETMVLMGMELPLSAIRSQIASGIDILIHLGRLRDKSRKVLKIAEIAGMEQGEV
                     MLHELFRYEESGEEARHVRGTLMRTGRLMHTEKCERAGMNPEKEGSSGL"
BASE COUNT          470 a          749 c          436 g          568 t
ORIGIN      
        1 ctcgaagata tgcctgctcc tcctgctttg tcagctcctg ctccggcaca tgcaccttgt
       61 aagctttggc ctttttcgcc ccatccacat acatacgcag gtctgcctcg tagtcacctt
      121 ctccattttc tctccgaagc agccccttct ccggatcctc atctgtctgc agttccatgt
      181 atccggacag cagcccgaga catgccgctg ccgcaatgac tgccgcccct ttctgccagt
      241 ctttcctgcc ccatttccgg ctgactgccc atccgagcac cgccagtcca agcgctattc
      301 cacatacaat cacattttcc ccctctttat ccacaaacct ttcgtcagat ctgaatattc
      361 aatatatgct ctgaccattt gatggcagca agatagcagc aaaaagcgat gctcatgaca
      421 gccactccga atgcgctgcc atacaaagcg tccagaaatt ctcccgatgc cacctgaaga
      481 tatgccagaa ttgcaagcgg cattatttcc ataatccgcc cctccagacg ctttcctgca
      541 agcacggttg ccacttccct ttcgacttca atccgccccg cgagtttttg taccgtcgtc
      601 tgcataatgt ggacaaagtc tccgccgctc cgctttgcaa acgcgaacac ctccgcaaaa
      661 ctctcgatct cctcacaccc gcttcgcgcc gcaaacgaaa gcaacagtcg ctccaatggt
      721 tcgttcatgc gcaccccctc attcatccag cgcacctctg ccgccataaa tcccttctcc
      781 ccatgcaatt ccagaagttc tttctccgtc tccctccatg cattttctac agaatatcct
      841 gccagcagcg ccgccgatgc agactgcatg gcatccttga actccatgag cagctgttcc
      901 tttctctttt ccttttgcag agtgcaatac tttttcacgc ataccaggta cgctgccgga
      961 aacagcagca ttccccacac agaccggtaa aacagccacg ctgccattcc tgccgctcct
     1021 gctcccactg ccgcacaagc cagtttctcc acaaaacctg cccggtattt tctatagtcc
     1081 actgctcccc tccttctccg ggttcattcc tgcacgctca catttttcgg tatgcatcag
     1141 ccggccggtt ctcatcaggg ttcccctcac atgccgtgcc tcctccccag actcttcata
     1201 cctgaacaat tcatggagca taacctcacc ctgctccatt cctgctattt ccgctatttt
     1261 taatacctta cggcttttat cccgcagcct tcccagatgg atcaggatat cgatccccga
     1321 tgcaatctga ctgcgtatcg cggaaagcgg cagttccatg cccatcagaa ccatcgtctc
     1381 caatctgctt aacatatcct tgcacgagtt ggcgtgcccg gtcgagagac ttccgtcatg
     1441 cccggtattc attgcctgca atacatccag cgcctccgcg cctctgcact ccccgacgat
     1501 aatgcgctcg gggcgcatgc gaagcgccgt ccggatcaga tcgcggatcg tgatcggcgt
     1561 aacgccttct gtattggcat tgcgcgtctc aagccgcacc agattcggct tgtccaaaag
     1621 ctgtagttcc gccgaatctt cgactgtaat gatacgttcc tcgcgaggaa tatactggga
     1681 aagtgcattc aaaaaggtag tctttccgga accggttccc ccggaaatga aaatattgta
     1741 gccgcaccgc accagtattt tcagtagatc tgccgcttcc tgtgagatgc ttcccatccg
     1801 gatgagatcc tccataccga tcggattttc cggaaacttt cggatcgtaa cgaccgaccc
     1861 gtcgattgct accggctcca gcacaatatt gacgcgggaa ccatccgcca gacgggtatc
     1921 gacaatcgga gatgcctcat tcaccatacg gttgctgtat cctgccatct gctggatcac
     1981 atcatttaac ttttctttgg aggaaaaccc gtactccgct ttgtacagcc gtcccgcttt
     2041 ctcgtagaag atatgttctg caccgtttac catgatctcc gtgatcgtcg gatcttccag
     2101 cagttcctgc agtacatcca gcttgcgcag ggaataaaac agagtctgta ccacagcttc
     2161 ccgctccccc agcgtaagca tcctctctct ggcaaagtcc cttacctgtg cttcgatcag
     2221 ctg
//
LOCUS       DBMDQJ010000416         2211 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17079_length_2211_cov_1.592891, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000416 DBMDQJ010000000
VERSION     DBMDQJ010000416.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2211)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17079_length_2211_cov_1.592891"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(259..1647)
                     /locus_tag="ACZO9R_08610"
     CDS             complement(259..1647)
                     /locus_tag="ACZO9R_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913159.1"
                     /translation="MARKKVAPEEEIVNAAGEEVIPESIPVDTVLSNEICTDTETSPS
                     DLPEGNLPAEESTEPSETLPAMEAAEAKPFGLSAAEKTASQEKNDTVEERAEEAELHE
                     ETSEFLEAAEDLYSDLPASAPQGSEADEADWGFEEPVTEEAAAEPAEDECLAADNAET
                     LDASEEKSERTLFYELDFNELDRGLSEEERKEWNSIYASFRGRSAITGTIIGVDLYAR
                     YLPRSEARMLENKRELCAVVVPYRIPILIRESEMWELGEERPDFVLRNMVGASIDVIV
                     TKVERTANRAQASRRQASRSQRRFFAAREDLHAVGSRITCRMLAVGPRRCLVDCYGYD
                     LDMTQREIRYAAIPDLRTEFHPGSEIDCIVKEYHPRTGELIVSAKETEVNPFFGAEER
                     HPVGSRRFAMISGKYGGGVFCNLPDGVTCMCNYSYQHEDADFMVGEHVMLMVQRYDQE
                     KLQMYGKIMSKW"
     gene            complement(1667..>2211)
                     /locus_tag="ACZO9R_08615"
     CDS             complement(1667..>2211)
                     /locus_tag="ACZO9R_08615"
                     /inference="COORDINATES: protein motif:HMM:NF039505.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5697 family protein"
                     /protein_id="HIR0913160.1"
                     /translation="LLSNQQKYILEILKEFKYLRVRQLHAMVRTHYRAQGIEIDARRM
                     EIMLRQMRTGTNYVWLRGDVVSYGDRRIDPRLLEALDVMLELTKAQPGFYSKDGLHEP
                     FLLRFTGNGKGAGYLFSVAWLDAATHIATVPRMKGERIIWISESNSFGEINLPRHHFF
                     AVRQEDGTHRFFGSNEPGKI"
BASE COUNT          474 a          640 c          560 g          537 t
ORIGIN      
        1 cgaaggccat tcgctttaag tcacatttac aaactgagcc tcaatcactc tactcggcgg
       61 cacagaagca aacttattcg tttttgccca tgtgttgtcc cctacaggca tcaagctgcc
      121 agatgccgcc agctcgtagg tgcgcagcat aagtatctga aaatcttaca ggactaaacg
      181 aaaaaggacc tctgccgaca cacaactgga tcggcagagg ccctcttttc ggaatgatat
      241 taaaccatgt gctgcttctc accacttgct catgatcttg ccgtacatct ggagcttttc
      301 ctgatcgtag cgctgcacca ttagcatgac gtgctcgccc accatgaagt ccgcatcctc
      361 gtgctgatag gagtagttac acatacaggt cacgccatca gggaggttgc agaacacgcc
      421 gccgccatat tttccggaga tcatcgcaaa gcggcgggag cctacagggt gacgctcctc
      481 cgcaccaaag aaggggttga cttccgtttc tttcgcggaa acaatcagtt caccggtacg
      541 ggggtgatac tcctttacaa tgcagtcgat ctccgagccc gggtggaact ccgtgcgaag
      601 atccgggatg gccgcgtagc ggatctctct ctgcgtcata tcaaggtcgt agccatagca
      661 gtccacaagg cagcggcggg ggccgaccgc cagcatcctg caggtaatac gggaaccgac
      721 agcatgaaga tcctcacggg cagcaaaaaa gcggcgctgg ctgcgactgg cctgacggcg
      781 ggacgcctgt gcacggttag cggtacgctc caccttggtg acaatcacat cgatgctggc
      841 ccccaccata tttcgaagca cgaagtcggg tcgctcctct cccagttccc acatttcgga
      901 ctcgcggatc agaatgggga tgcgatatgg caccacaacg gcacagagct cccgcttatt
      961 ttcaagcatt cttgcctcgc tgcgcggaag gtatcttgcg tagagatcca caccgatgat
     1021 cgttccggta atggcgctgc ggccgcggaa agatgcatag atggagttcc attctttccg
     1081 ttcctcttcg ctcagccccc gatccagttc attgaagtcc agctcgtaga agagcgtgcg
     1141 ctcgctcttt tcttccgacg catcaagcgt ttcggcgtta tctgctgcca ggcattcatc
     1201 ctccgcaggt tctgcagcgg cttcttccgt gaccggctcc tcaaagcccc aatccgcttc
     1261 atcggcctca gaaccctgcg gcgcagatgc gggcagatct gaatacaaat cctctgctgc
     1321 ctcaagaaat tccgacgtct cttcgtgcag ttccgcttct tccgctcgct cctccacggt
     1381 atcatttttt tcttgggatg cagttttttc tgctgcagat agtccaaacg gcttcgcctc
     1441 ggctgcctcc attgcaggaa gcgtctccga cggctccgtc gattcctccg caggaagatt
     1501 cccctccggc agatctgagg gagatgtttc ggtatctgtg cagatctcat tagatagtac
     1561 ggtatccaca gggatactct ccgggataac ttcttcaccg gcagcattta caatctcttc
     1621 ttccggggca acttttttcc ttgccatata atattcctcc taagtgttat atttttcccg
     1681 gctcattgga gccaaaaaag cgatgggtgc cgtcctcctg acggacggcg aaaaagtggt
     1741 gccggggcag attgatttct ccaaaggaat tgctttcgga gatccagatg atccgctcgc
     1801 ctttcatacg ggggacggtt gcaatgtgag tagcggcatc cagccatgcc acgctgaaaa
     1861 gatatccggc tcctttgccg tttccggtaa agcgcaggag aaacggttca tgcagtccgt
     1921 cttttgagta aaatccgggc tgcgcctttg tcagttccag cataacgtcc agtgcttcca
     1981 aaagccgagg gtctatcctg cggtctccgt aagagaccac atctccccgc agccagacat
     2041 aattggtgcc tgtgcgcatc tggcgaagca taatctccat gcgtcttgcg tcgatctcga
     2101 ttccttgcgc gcggtagtgg gttcgcacca tggcatgaag ctggcggaca cgcaggtact
     2161 taaactcctt gagtatttcg agaatatact tttgctggtt ggatagcagc a
//
LOCUS       DBMDQJ010000417         2210 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17092_length_2210_cov_1.495970, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000417 DBMDQJ010000000
VERSION     DBMDQJ010000417.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17092_length_2210_cov_1.495970"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(90..869)
                     /locus_tag="ACZO9R_08620"
     CDS             complement(90..869)
                     /locus_tag="ACZO9R_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913161.1"
                     /translation="MDGQQNNQNGWNGSEAPRTYGEDTKMYQNRQNAPQQPNYGSGQN
                     PYQQPNYGNSQNAYQQPDYGNAQNMNQQPNYGNSQNAYQQPNYGNGQNPYQQPNYGNW
                     QGNPYPQEQHGPVSDVLCNILLVIFMAQIIINFVVFGATWNAMAGEGIDAYLDGSVSL
                     GSPVQMLSMFSNLLFVAMLVLLILDIIKISNQKYKITGLVLFAIFLRPGYYIWRAHVL
                     KRKMTFPIIYTIAYSGVTVAYFFYSFYKVFSLTFGIMQSLN"
     gene            complement(892..1770)
                     /gene="rsmA"
                     /locus_tag="ACZO9R_08625"
     CDS             complement(892..1770)
                     /gene="rsmA"
                     /locus_tag="ACZO9R_08625"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR0913162.1"
                     /translation="MATLGIPQNTITVLQKYHFNFQKKFGQNFLIDTHVLERIIEAAG
                     ITKDDFVLEIGPGIGTMTQYLCENAREVTAVEIDQNLIPILADTLSAYDNVTVINEDI
                     LKLDIVKLAEEKNAGKPIKVVANLPYYITTPIIMGLFESHVPIDSITIMVQKEVADRM
                     QVGPGTKDYGALSLAVQYYAKPEIIANVPPNCFMPRPNVGSAVIRLTRHETVPVQVED
                     EKLMFRLIRASFNQRRKTLANGLNNSPEIHLSKEVIQESIEELGVPVTIRGEALTLEQ
                     FAALSNIIGRRMKQQV"
     gene            complement(1758..2177)
                     /locus_tag="ACZO9R_08630"
     CDS             complement(1758..2177)
                     /locus_tag="ACZO9R_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HIR0913163.1"
                     /translation="MDTDIFEQFPDRETFDKYWNENYQPVTYEDVRETFTDFVKSADG
                     HIYLSDYEEKGLISREDFKENLSQEAQFTFEDGLTEVFYDKNPELYETAFALYEESKL
                     TGKGDASVAQTFHETFRALYAEFLDRLYDEVLAAWQH"
BASE COUNT          515 a          636 c          454 g          605 t
ORIGIN      
        1 tagtatttcc attttttccg cataaaaaaa tctccttgtc cattattata tgaaccaagg
       61 agatcttcca ttcctacaaa gccgcggcac tagttcagac tctgcataat cccaaatgtc
      121 agtgaaaaaa ctttataaaa cgaatagaaa aagtatgcca cggtcactcc gctgtaagcg
      181 atggtataga tgatcgggaa cgtcattttt ctcttaagca catgtgctct ccagatgtaa
      241 tagcccggtc ttaagaaaat cgcaaacaac accagaccgg tgatcttgta tttctgattg
      301 ctgattttta taatatccag aatcaacagc acaagcatcg ccacaaacag cagattggaa
      361 aacatggaaa gcatctgcac cgggctgcca aggctcacgg atccgtcgag ataagcgtct
      421 attccttccc ctgccatcgc attccatgtc gcgccaaata ccacaaaatt gataataatc
      481 tgcgccataa agatcaccag gagaatattg cacagaacat cggataccgg tccgtgctgc
      541 tcctgcggat acggattgcc ctgccagttt ccgtaattcg gctgctgata cgggttctga
      601 ccgtttccgt aattcggctg ctgatatgca ttctggctgt ttccgtaatt cggctgttga
      661 ttcatgttct gtgcatttcc gtaatccggc tgctgatatg cattctggct gtttccgtaa
      721 ttcggctgtt gatacgggtt ctgaccgctt ccgtaattcg gctgctgcgg cgcgttctgc
      781 cggttctgat acattttcgt atcctcaccg tatgttctgg gcgcctcgct cccgttccat
      841 ccattctgat tgttctgctg tccatccatt gtctgtttcc ccctattctc tctatacctg
      901 ctgcttcatt ctgcgtccga taatattcga gagcgccgca aactgttcca atgtaagcgc
      961 ctctccacgg atggtcaccg gcacgccaag ctcctcaatg ctttcctgaa tcacttcctt
     1021 ggagagatga atctccggcg aattattcag accgttcgca agcgtcttgc gtctctggtt
     1081 aaaagatgcc cggatcagcc ggaacatcag cttttcatcc tccacctgca ccggcacggt
     1141 ctcatgacgc gtcagcctta tcaccgcgct gccgacattc ggccgcggca taaagcagtt
     1201 cggcggcacg tttgcgataa tctccggctt tgcataatac tgcaccgcca gcgagagcgc
     1261 accgtaatcc ttcgtccccg gtccgacctg catcctgtct gcgacttcct tctgcaccat
     1321 gatcgtaatg ctgtcaatcg gcacatggct ctcaaacagc cccatgataa tcggcgtagt
     1381 aatataatac ggaagatttg ccaccacctt gatcggtttc ccggcattct tctcctccgc
     1441 gagctttaca atatccagct tcaaaatgtc ctcattgata accgtcacat tgtcataagc
     1501 gctaagcgtg tccgccagaa tcgggatcag gttctgatcg atctccaccg ccgtcacctc
     1561 tctggcattt tcacacagat actgggtcat cgtgccgatg cccggtccga tctccagcac
     1621 aaagtcatcc ttcgtaatgc ccgccgcctc aataatgcgc tcgagcacat gggtatcaat
     1681 gagaaaattc tgtccgaact ttttctgaaa attaaaatga tacttctgca gaaccgtaat
     1741 cgtattctgc gggattccta atgttgccat gctgccagca cctcatcata cagtctgtcc
     1801 agaaactctg catagagcgc gcgaaacgtc tcgtgaaacg tctgcgccac cgatgcatct
     1861 ccctttccgg taagctttga ctcctcatag agcgcaaacg ccgtctcata aagctccgga
     1921 ttcttatcgt aaaaaacttc cgtcagtccg tcctcaaatg taaactgtgc ctcctgcgag
     1981 aggttttcct taaaatcttc ccgggagatc agccccttct cctcgtaatc cgagaggtag
     2041 atgtgaccgt ccgcagactt cacaaaatcg gtgaacgtct cgcgcacatc ctcgtatgtc
     2101 accggctgat aattctcgtt ccagtatttg tcaaatgttt cccgatccgg gaactgctca
     2161 aaaatatctg tatccatctt tctccattcc aaagcccgtc cgggggcttc
//
LOCUS       DBMDQJ010000418         2206 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17131_length_2206_cov_1.277910, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000418 DBMDQJ010000000
VERSION     DBMDQJ010000418.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17131_length_2206_cov_1.277910"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /gene="fliM"
                     /locus_tag="ACZO9R_08635"
     CDS             complement(<1..779)
                     /gene="fliM"
                     /locus_tag="ACZO9R_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HIR0913164.1"
                     /translation="MGEVLSQNEIDSLLKALSSGELDVDEIKDDNEKQVKNYDFARPA
                     KFSKEHLRTLEIIFEHYGRLLSTNLPVYLRKNVQVEVMSSEAVTYSEFANALSNPVLL
                     GIVNFAPLNNNIIIEIAENVGFTIVDRMLGGQGAPLDKTREFSEIELLLIERIYNVCV
                     NLLVEPWASVCELTPRLERIETNSQYAQIISPTEMIALITLNIKIGDVEGLINICLPY
                     ITLESVMDKLNTKYWYSSLQEKDEQQYTETIEALIARAPMP"
     gene            complement(806..1339)
                     /locus_tag="ACZO9R_08640"
     CDS             complement(806..1339)
                     /locus_tag="ACZO9R_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HIR0913165.1"
                     /translation="MKKNLLAVIILALVLVNLILTAVLVFSIIPQTKKSNELIDQVCA
                     AINIELEGGQNKDSSAVPIEEIAVYNITDNFTVNLADNGDGKKHYAVFSVGLSVNKTS
                     ESYATYGGDEMTELTDKETIIRSDINTVVAKYTEEEFNADGQKAVKEEILSKMQDLFG
                     SDYIVGVNFSSVATEAH"
     gene            complement(1353..>2206)
                     /locus_tag="ACZO9R_08645"
     CDS             complement(1353..>2206)
                     /locus_tag="ACZO9R_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR0913166.1"
                     /translation="FFVMLFSMSSVNEEKFEKVIASFQSTFSILPGGGASIGEGELIS
                     SGISQLENFDSYYNQQLSSQSDGQTEEEKDITEAYEQQELEESEDMAQQLENALSQYG
                     IQDDVEVDFNAEYVTLNMNGALLFDSASAELRDEAYPLVNKLGKILVTYDNNIIEVEG
                     HTDNVPIHSSKYEDNNVLSMYRALAVANYLRDTTTLDPAYIKSSGRGEYVPIADNATP
                     EGRARNRRVEIKIYNSYNSNVSGTSTDDTGTETPADAALSTETVTDTPEAATTGATVE
                     PTEVVNE"
BASE COUNT          518 a          602 c          438 g          648 t
ORIGIN      
        1 atcggcatcg gtgcccgtgc aataagtgcc tcaatggtct ctgtatattg ctgttcatcc
       61 ttctcctgca ggctggaata ccagtatttt gtattcagct tgtccatcac actttccaat
      121 gtgatatacg gcaggcagat gttgatcagt ccctcgacat caccaatctt aatattcaac
      181 gtaattaacg cgatcatctc cgtcggggaa atgatctggg cgtactgcga attcgtctca
      241 atccgctcca gtctcggtgt cagttcacac acactcgccc acggttccac cagaagattc
      301 acgcatacgt tatagatccg ctcaatcaga agaagctcga tttcggagaa ctccctcgtc
      361 ttgtcaagcg gcgccccctg gccgccgagc atacgatcca cgatcgtaaa gccgacattc
      421 tctgcaattt caataatgat attgttattc agcggagcaa aatttacaat tccaagcaac
      481 accggattcg atagtgcatt cgcaaactct gaatacgtca ccgcctccga actcatcact
      541 tccacctgaa cattttttct taggtacacc ggcagattcg ttgaaagcaa tcttccataa
      601 tgttcaaaaa taatctcaag cgttcgcaaa tgctcttttg agaacttcgc gggacgggca
      661 aaatcgtaat ttttgacctg tttttcatta tcatctttta tttcatctac atccagttca
      721 ccgctgctaa gagctttcag caaactgtct atctcatttt gagataagac ttcacccatt
      781 ctctctcacc acctactgtc ttattctagt gtgcttccgt tgccacgctt gagaaattca
      841 cgcccacgat atagtcggag ccgaacagat cctgcatctt ggataagatc tcctccttca
      901 cagccttctg tccatctgcg ttgaactcct cctcggtata cttggccact accgtattga
      961 tgtcgctgcg gataatggtc tccttatccg taagctctgt catctcgtct cccccgtagg
     1021 tggcatagga ctcgcttgtc ttgttgacgg aaagtcctac agaaaataca gcgtaatgct
     1081 tctttccatc gccgttgtct gccagattca cggtaaaatt atctgtgata ttataaactg
     1141 caatttcttc gatcggcact gcggaactgt ctttgttctg tccgccctcg agttcgatgt
     1201 tgattgcagc gcacacctga tcaatcagtt cgtttgactt tttggtctgc ggaataatgg
     1261 aaaataccag caccgcggtc agaatcagat tcacgagaac cagtgccaaa ataattactg
     1321 ccaataaatt tttcttcatt atgcttccct tcctattcgt tcacaacttc tgtcggttcc
     1381 acagtcgcac ctgttgtcgc tgcttccggc gtatctgtca ccgtctcagt gcttaaagcc
     1441 gcatccgcag gcgtctctgt acctgtgtca tccgtggatg tcccgctcac attcgagttg
     1501 tatgagttat agattttaat ctcaacccgg cggttccttg ccctgccctc cggcgttgca
     1561 ttatctgcaa tcggcacata ctctccgcgg ccagacgact ttatgtatgc aggatccagt
     1621 gttgtggtat cccgcagata gttggcaacc gcaagcgcac ggtacatgga cagtacgttg
     1681 ttgtcctcat acttggaaga atgaatcggc acattgtccg tgtgtccctc gacctcaatg
     1741 atgttattat cgtaagtaac cagtatcttg ccaagcttgt tgaccagcgg atacgcctca
     1801 tctctgagtt ctgcgcttgc agaatcaaac agcagcgcgc cgttcatgtt aagcgttaca
     1861 tactctgcat taaaatcaac ctcgacgtcg tcctgtatcc catattgcga aagtgcattt
     1921 tcaagctgct gcgccatatc ctcagactct tccagttcct gctgttcata tgcttcggta
     1981 atgtcttttt cttcctccgt ctgtccgtca gactggctgc tcagctgctg gttgtaataa
     2041 gaatcaaaat tctcaagctg actgattccg gaagaaatca attcaccctc gccgatcgat
     2101 gctccgcctc ccgggagtat gctgaatgta ctctggaaag aggcaatgac cttctcaaac
     2161 ttttcctcat tcacactgga catggagaaa agcattacga agaaac
//
LOCUS       DBMDQJ010000419         2202 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17165_length_2202_cov_1.198953, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000419 DBMDQJ010000000
VERSION     DBMDQJ010000419.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17165_length_2202_cov_1.198953"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(5..331)
                     /gene="trxA"
                     /locus_tag="ACZO9R_08650"
     CDS             complement(5..331)
                     /gene="trxA"
                     /locus_tag="ACZO9R_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR0913167.1"
                     /translation="MAVVTITNENFEDEVLAAKVPVILDFWAEWCGPCKMQSPVFEAL
                     SEELAGKVKFGSVNVDEQAALALKYQVMSIPMLVVMQYGIFKKKAIGLQTREEILDLL
                     ACCEAE"
     assembly_gap    525..534
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(535..1985)
                     /locus_tag="ACZO9R_08655"
                     /pseudo
     CDS             complement(535..1985)
                     /locus_tag="ACZO9R_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019903.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
BASE COUNT          539 a          663 c          426 g          564 t
ORIGIN      
        1 ttatctattc tgcttcgcag catgcaagca gatctaagat ttcctctctc gtctgcagtc
       61 cgatcgcttt tttcttaaaa atgccgtact gcatcacaac gagcatcgga atgctcatca
      121 cctgatactt taacgcaagc gccgcctgct cgtccacatt gacgcttccg aacttcacct
      181 ttccggcaag ctcctcggac agcgcctcaa ataccggcga ctgcatttta cacggtccgc
      241 accactctgc ccagaaatcc agaatgaccg gcacctttgc cgccagcacc tcgtcctcaa
      301 aattttcatt tgtaattgta acaactgcca tattctgctt cctttctgtg cattccgccg
      361 gcactgtttt ctgccggcag ctgctctgac acacgctcta ttccctgccg aactccgaga
      421 gtaccagacc ttcgttccgg tctaatacca tgccgacact ccatgcattg acaaacagta
      481 acagcggtct gcccttcaga tcctgaatct tcttcatatc cgagnnnnnn nnnnttatcc
      541 atatcgatgt cctcattctc aatccaccgg atatgctcat acggaagcgt atccatgcgg
      601 atttccacat tgtactcatt ctcgaggcgg tatttcaaca catcgaactg cagtacaccg
      661 accacgccga cgatgacctc ctccattccc atattgtact cctggaaaat ctggatcgca
      721 ccttcctgcg caatctgctg catacccttc atgaactgct tgcgcttcat cgtatccacc
      781 tgccgcactc tcgcaaaatg ctccggtgcg aacgtcggga ttccctcgaa tttgaacggc
      841 ttcgccgtgg tgagtgtatc tccgatggaa aaaatacccg gatcaaacac accgatgatg
      901 tctcccgcat acgcctcgtc cacgatatga cgctcctgcg ccatcatctg ctgcggctgc
      961 gaaagcttga tctttttatt gccgccctgc acatgtgtca cttccatgcc ggcggtaaac
     1021 ttgcccgagc agatgcgcat aaacgcaatt cggtcacggt gtgccttgtt catgtttgcc
     1081 tgaatcttga atacaaacgc agagaaatcc tcaccaaacg gatcaacttc tccgatatcc
     1141 gccttgcgcg gcagcggcga gtaagtcatc tttaagaaat gctgcaaaaa agtctccaca
     1201 ccgaaattcg tcaatgccga accgaaaaat accggtgaaa gctctccctt cgtcacaagt
     1261 tcctgatcaa actccgcgct cgctccgtcc agaagctcga tctcctccat cagcttatca
     1321 tacgcatcct gtccgatctc tgcaatcagc gccggatcgt ccacggaaat atgcttctcc
     1381 gtaccttcct tggttccctt taatgtatcg gaaaacgtca taatctctct tgtgttgcga
     1441 tcatagacac ccttaaaatt cttaccggaa ccgatcggcc agttgatcgg gcaggtcgca
     1501 atgccaagct ccttctcgat ctcatcaaga agatcaaacg tgtcgttcgc atcgcggtcc
     1561 atcttgttga tgaatgtaaa aatcggaata tggcgcatca cacagacctt gaacagcttt
     1621 ctggtctgcg cctcgacacc cttggaaccg tcgattacca tgacggcaga atccgccgcc
     1681 atcaaggtac ggtaagtatc ctccgagaag tcctgatgtc ccggtgtatc aagaatattg
     1741 atgcagtaac ctccgtaatt gaactgcagt accgatgacg tgacggaaat acctctctcc
     1801 ttctcaatct ccatccagtc ggacactgca tgacgcgccg tcgcttttcc cttgacggaa
     1861 cccgccagat tgattgctcc tccatagagc aggaacttct ccgtcagcgt cgtcttaccg
     1921 gcatccgggt gggaaataat tgcaaacgta cgtctctttt caatctcttt tcttaaatca
     1981 gccaaactat ctatcctcca aaaaacaaat cacttcaaaa atgccaacgc agcaaagccg
     2041 aaactttgca tacgttggca ctataacata atttcatgaa aaatgcaaaa acttctcttc
     2101 cgaagatctg tactatttct taaataccgg aatcgcctcg cccgcgcact ccggggtgag
     2161 atcaaagtag gacagaattg tcgctgcaat gtcggaaaag ct
//
LOCUS       DBMDQJ010000420         2199 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17199_length_2199_cov_1.648236, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000420 DBMDQJ010000000
VERSION     DBMDQJ010000420.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17199_length_2199_cov_1.648236"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..2077
                     /locus_tag="ACZO9R_08660"
     CDS             <1..2077
                     /locus_tag="ACZO9R_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913168.1"
                     /translation="ILDDVFASNMEKGIQMWRSLLNIAEPSLRTDAKTAEKLLPDWDW
                     LDSPSHDQVLLLLAALNDERFVSQLFESASIGRLQLDVLNACRDCGQEKQGQHCLELA
                     LKNPYLEESWEKRFRRVFTTTPSSRIEKPHSHATAKPIADAKPDDGTVYHYCSVQVQG
                     TRRPYAYLTGGLPLKVGDWVELPFGKDDVLRQGQIKAVMDCTRMVAPWPPEQTKAIIR
                     VVEAPAEAEKQVLPSKPESPSELSPKPTQKKEPVLASTPKAATALKIPPASASTSPNM
                     GSEMVQFPGKVEAGKGHKKGVPDEPEIIPATIPKRKSSRKFICCSITLFACIIAGVIF
                     CRSIAAHYQQAERNLAENNFQSAVIELSRVPFFYRNQKSLSRYADLCLMAESGTKEDL
                     KTALDGLESLLADSDEALNAQMQPQYESIYKQYYDLLYQAALDYLQNARFAQAVEYLQ
                     QIPPEYPCIVELLCYARAQTDVDNSDTSHHLKTVLSSLEQIPAEYDGPFSEDIVAFRA
                     DLLDMITDAETREEQAKAEREAAQAAAKKAEEDRIAALKASGIPYIGMAEREVNSTRQ
                     LGKAAYNDQNSGEWVKGADGKYFYDTKQVYAWYSKQNGDMVFKVECKNGVVISAVKYG
                     GDTYWNGNQLLVKLGPQIIHKFNSGGTTNSGSDSSIRDEYDNPEDLYEENRDWFDDED
                     EAWDYWYED"
BASE COUNT          623 a          491 c          576 g          509 t
ORIGIN      
        1 tattctggat gatgtgttcg caagcaacat ggaaaagggc atccagatgt ggcgaagcct
       61 tttgaacatc gcggagccgt ctctgagaac cgatgctaaa actgccgaaa aacttctccc
      121 ggattgggat tggctggatt ctccgagcca tgatcaagtg ctgctgcttt tggccgcatt
      181 gaacgacgag aggtttgtat cacagctttt tgagagcgca tccataggca gattacagct
      241 tgatgttctg aatgcctgtc gtgactgcgg gcaagagaaa cagggacagc attgccttga
      301 gcttgcactg aaaaatccat atcttgaaga aagctgggaa aaacggttca gacgagtgtt
      361 caccacaaca ccgtcttcca gaatagaaaa gccgcattct catgcgaccg caaagcccat
      421 tgcagacgca aaacctgacg acggtacggt gtatcactat tgctcggttc aggtgcaggg
      481 aacgcgccgt ccgtatgcgt atctgaccgg cggtttgccg ctcaaggtcg gagattgggt
      541 cgagcttccg tttgggaaag acgatgtgct gcggcaggga cagataaaag ccgttatgga
      601 ctgtacgcgc atggtcgcgc cttggcctcc ggagcaaacg aaggctatta tccgtgtggt
      661 ggaagcacca gcagaagcag agaagcaggt attaccatct aagccagaat caccttcgga
      721 actatctcca aaaccgacac agaagaaaga acctgtgcta gcttctacgc caaaggcggc
      781 aacagcacta aaaattccgc ctgcatcagc gtcaacatca cccaatatgg gcagtgaaat
      841 ggtgcagttt ccgggaaaag tcgaggcagg aaaaggtcac aagaagggcg ttccggatga
      901 accagaaatc attcctgcta ccattcctaa acgcaagtcc tcgcggaaat tcatctgctg
      961 tagtattacg ctttttgcgt gcatcatagc tggtgtgatt ttttgcagat ctatcgctgc
     1021 tcattaccag caggcagaac ggaatctcgc agaaaataac ttccaaagtg ctgtaattga
     1081 attaagccgc gttccctttt tctatcggaa tcagaaatcg cttagccgct atgcagatct
     1141 gtgtctcatg gcagagagcg gaacgaaaga agacctcaaa actgcacttg atggtctgga
     1201 atcactgttg gccgacagtg atgaagcatt gaatgcacag atgcagccac agtatgagag
     1261 catctataag cagtattatg atttgctcta tcaagcagca cttgactatt tgcagaatgc
     1321 tcgctttgcg caagcagtgg aatatctcca gcagattccg ccggaatacc cttgcattgt
     1381 ggaactgctg tgctatgcac gggcgcagac tgatgttgat aattccgata cctcgcatca
     1441 tctaaaaact gtattatcct ctttggaaca gattcctgcg gaatacgatg gccctttttc
     1501 agaagatatt gttgcgttca gagcagatct tttagatatg attacggacg cagaaaccag
     1561 agaggaacag gcaaaggctg aaagagaagc ggctcaggcc gcagcaaaga aggcagagga
     1621 agacagaatc gccgcactaa aagccagtgg tattccctat attggtatgg ctgagcgtga
     1681 ggtgaattct acccgccagt taggaaaagc ggcgtacaat gaccagaata gcggagagtg
     1741 ggtaaagggc gctgatggca agtattttta cgatacaaag caggtctacg cctggtacag
     1801 caagcagaac ggagatatgg tctttaaggt cgaatgtaaa aatggtgttg tgataagcgc
     1861 cgtgaagtac ggcggtgata cttactggaa cggtaatcag cttcttgtga agctcggccc
     1921 gcagatcatc cacaagttca attctggcgg taccaccaat agcggaagtg actccagcat
     1981 ccgtgatgag tatgacaatc cggaagacct gtacgaagaa aaccgggact ggttcgatga
     2041 tgaggatgaa gcttgggact attggtatga ggattgatat acacatttct aattgcagag
     2101 cctaataagc aacggcccat cggctccgaa atttatcgga gccgatgggt atgtctttat
     2161 tgggcacacg gaatcggtat taaaataaac ctctttaga
//
LOCUS       DBMDQJ010000421         2183 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17362_length_2183_cov_1.311239, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000421 DBMDQJ010000000
VERSION     DBMDQJ010000421.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17362_length_2183_cov_1.311239"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="ACZO9R_08665"
     CDS             <1..960
                     /locus_tag="ACZO9R_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0913169.1"
                     /translation="LSVIYQTDLETVYAVNGVSFTLGRGESLGLVGETGAGKTTLALS
                     IMQLLPERTGKVTGGEIWFEGEELTSLSEQKMRTIRGNKVAMIFQDPMTSLNPVLTVG
                     EQIAEALLVHNEAGWTKEKIDQHVDETLNMVGIPPARKHEYPHQFSGGMKQRVVIAMA
                     LSCEPDLLIADEPTTALDVTIQAQVLLMIRELRDRLGTSMIMITHDLGIVAQTCDNVA
                     VMYAGEIIEIGTAEDIFNGKNHHPYTEGLFGSIPRLNTRVKRLTPIDGLMPDPTVCEP
                     GCKFAPRCSKCMDICREKSPEVHYEGTHAIRCHLFQQTGQIGE"
     gene            966..1928
                     /locus_tag="ACZO9R_08670"
     CDS             966..1928
                     /locus_tag="ACZO9R_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0913170.1"
                     /translation="MEVNTPLIEAKALKKYFKLGENRYLHAVDNINLQIYKGTTVGIV
                     GESGCGKSTFGRTLLRLTEATSGEILYCGENILTFGRQKMKQMRKNMQIIFQDPYASV
                     DPRKSVVEIIAEYMLIHHTYPTRREIFNRAAQLMDIVGLARRTANSYPHELDGGRRQR
                     IGIARALSLNPQFIVCDEPVSALDVSIQAQILNLLMDLQSEMDLTYMFVTHDLSVVRH
                     ISDHIMVMYLGESLEYAEADELFNSPLHPYTRALLSSIPEPDISMRNKKISVIKGEVV
                     SPINPKPGCRFAPRCENCMEICTQCSPELKEVAPGHLVACHKYA"
     gene            1935..>2183
                     /locus_tag="ACZO9R_08675"
     CDS             1935..>2183
                     /locus_tag="ACZO9R_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913171.1"
                     /translation="MKITKSDDMPKGGTPYMPSQYLLDAYEPRRYMRYFEEITRIPHD
                     TEDEAHLAAYIREVAKTAGLESYQDPHGNVLVRMPATEG"
BASE COUNT          583 a          511 c          608 g          481 t
ORIGIN      
        1 ttaagcgtga tttaccaaac ggatcttgaa acggtttatg cggtgaacgg agtttcgttt
       61 acgctgggac gaggagaaag ccttggactg gtcggcgaga cgggagccgg gaagactacg
      121 ctggcacttt ccataatgca gctgcttccg gaacggacgg gaaaagtgac cgggggagag
      181 atctggtttg aaggagaaga gctgacgtcc ctttccgaac agaaaatgcg cacgatacgc
      241 ggaaacaagg tcgccatgat ttttcaggat ccgatgactt ccttgaaccc cgttctgacg
      301 gtaggcgagc agatcgcaga agcgctgctg gtgcataatg aagccggctg gacaaaggaa
      361 aagatcgacc agcacgtaga cgaaacgcta aatatggtag ggattccgcc ggcccggaaa
      421 catgagtatc cgcatcaatt ctccggagga atgaagcagc gggtggtgat cgcgatggcg
      481 ttgtcctgtg agccggatct gcttattgca gatgagccga cgactgcgct ggatgtgacg
      541 atccaggctc aggtcctgct tatgatacga gagctgaggg accggctggg tacgtctatg
      601 atcatgatca cgcatgacct gggcattgtg gcgcaaacct gcgataacgt ggcggtgatg
      661 tatgcgggcg aaatcattga gatcggtacg gcagaagaca ttttcaacgg gaaaaaccat
      721 catccctata cagaagggct ttttgggtca attcctcgtc tgaacacaag agtcaaacgc
      781 ctgaccccga tcgacggcct gatgcctgat ccgacggtgt gcgaaccggg ctgcaaattt
      841 gccccccgct gctcaaaatg catggacatt tgccgggaga agagtccgga ggtccattat
      901 gagggtacac acgcaattcg ttgccatttg tttcaacaga ccggacagat aggagagtag
      961 tgccaatgga agtaaataca cccttgattg aagcaaaagc attaaaaaag tatttcaaat
     1021 tgggggaaaa ccgctatttg catgctgttg acaatatcaa cctgcagatc tataaaggca
     1081 ctacggtggg aattgtagga gagtcaggat gtggaaaaag cacatttggg cgtacgcttc
     1141 tgcgtctgac cgaagcgacc agcggagaga tactctattg cggagagaac atcctgacgt
     1201 ttgggcggca gaagatgaag cagatgcgca aaaatatgca gatcatcttt caagacccgt
     1261 atgccagtgt agatcctaga aaatccgtgg ttgagattat tgccgaatat atgctgatcc
     1321 accacaccta tcctacccgg cgggagatct ttaaccgggc agcgcagctg atggatattg
     1381 tgggcttggc cagacggact gcgaattctt atccccacga attggacggc ggcagacggc
     1441 agagaatcgg catcgcgcgg gccttatccc tgaatccgca gtttattgtg tgcgatgaac
     1501 cggtttcagc gctcgatgtt tctattcagg cacagatcct gaatctgttg atggatcttc
     1561 agagcgagat ggatctcacc tatatgtttg ttacccacga tctgagtgta gttagacaca
     1621 tttcggatca tatcatggtc atgtatttag gtgagagtct ggagtacgcc gaagcggatg
     1681 aactgttcaa ctctccactt catccctata cccgtgcgtt gctttcttct atccccgaac
     1741 cggatatttc catgcggaac aagaagatct ccgtgatcaa aggcgaggtc gtcagcccga
     1801 tcaaccccaa gccgggctgc cgattcgcgc cccgttgtga aaactgcatg gaaatctgca
     1861 cacaatgctc ccccgaactg aaggaggtgg ctccgggaca tcttgtcgcg tgccacaaat
     1921 atgcttgaga ccatatgaaa ataacaaaaa gcgatgatat gccgaaagga gggaccccct
     1981 atatgccgtc acaatatctg cttgacgcat atgaaccccg gagatatatg aggtattttg
     2041 aggaaatcac gaggatcccg catgataccg aagacgaagc acatcttgca gcatacatca
     2101 gagaagtcgc aaaaaccgca gggctggagt cgtatcagga tccgcacggg aacgtgttag
     2161 tgcgtatgcc tgcaacggag gga
//
LOCUS       DBMDQJ010000422         2183 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17368_length_2183_cov_1.175793, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000422 DBMDQJ010000000
VERSION     DBMDQJ010000422.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17368_length_2183_cov_1.175793"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>412
                     /locus_tag="ACZO9R_08680"
     CDS             <1..>412
                     /locus_tag="ACZO9R_08680"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0913172.1"
                     /translation="IVVCLDDDGQTPANEVGKLLEGIEQGSDVVYARYAHKHHSGFRN
                     WGSHVNELMTRVMLGKPKELYVSSYFAARRFVVDEMSRYTYAFPYVIGLVLRTTKNIT
                     NVDVNHRDREAGVSGYTLGKLLNLWFNGFTAFSV"
     assembly_gap    413..422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <423..749
                     /locus_tag="ACZO9R_08685"
     CDS             <423..749
                     /locus_tag="ACZO9R_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913173.1"
                     /translation="AGTVCAIAGFLYGIYTIIKKLFFNPPGLVTGFSALMSVLVFMGG
                     MLMLMLGLVGEYIGRMYVSMNNSPQYVIRQMTFREKPSGKDGGRMQNTGKIQAGNTEE
                     IQAGES"
     gene            749..>1686
                     /locus_tag="ACZO9R_08690"
     CDS             749..>1686
                     /locus_tag="ACZO9R_08690"
                     /inference="COORDINATES: protein motif:HMM:NF039324.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HIR0913174.1"
                     /translation="MGTWIFPMENYLDFSNSILPVLIALLVFMVTRKLRPGKPTVISV
                     LFGFLFSICMVFGAQLDQKGSVPFMNPWMWLSVLAFAVVMTLMVSGLWSAMAQRQQSR
                     VTLPHLKASREICGVSKTQTGRTEGGAFFLLRTGGVIFLLYFVVFLAVYPGFFVYDAQ
                     EEYLEVVTRSFTTHHPLFHVLMLGGIVQLVYKLTGSINLGIAAYTLFQMAALSLIFGY
                     FIWKLGEHGLRKRGQWILTLYLGICPPLVMFSLCSAKDGLFMGMLLIQVLLLLDLCED
                     VDAFGADRKKMLLFAVSAILMMLFRHNGCYAFLVFLP"
     assembly_gap    1687..1696
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1697..>2183
                     /locus_tag="ACZO9R_08695"
     CDS             <1697..>2183
                     /locus_tag="ACZO9R_08695"
                     /inference="COORDINATES: protein motif:HMM:NF039324.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6020 family protein"
                     /protein_id="HIR0913175.1"
                     /translation="FLAAFVKKKAFSGNDRIQAGRRSARLLAYGVLLICLYLILNKGL
                     AFATKASGGEHQEMLTVPIMQLARTYAYEPDAFTEEDREVLFRYLPEDALQRYTPKVS
                     DGVKISFSNAAYEENSGDFWKLWAGMLIHHPFSYVNAWLMTSYGFWYPDTVIDVYRGN
                     TV"
BASE COUNT          498 a          433 c          612 g          620 t
ORIGIN      
        1 acattgttgt gtgtctggat gatgatggac agacaccggc gaacgaagtg ggaaagctgc
       61 ttgaggggat tgaacaggga tccgatgtgg tgtatgccag atatgcgcat aagcatcatt
      121 ccggcttccg taactgggga agccatgtga atgagctgat gacgagagtg atgctcggaa
      181 agccgaagga gctctatgta tccagttatt ttgcagccag acgctttgtg gtagatgaaa
      241 tgagtcgtta tacctatgca tttccttatg tgatcggtct tgtgctccgt accacgaaga
      301 atattacgaa tgtggatgta aatcaccgtg accgggaagc aggagtttcc ggatatacac
      361 tgggaaagct tttgaatctc tggtttaacg gttttacggc attcagtgtg aannnnnnnn
      421 nngcaggaac ggtttgtgcc attgcagggt ttttatatgg aatttataca attattaaaa
      481 agctgttctt taatccgccc ggactggtta ccggcttcag cgcactgatg tctgttctgg
      541 tttttatggg tggaatgctc atgctgatgc tgggacttgt tggagaatac attggaagaa
      601 tgtatgtgtc catgaataat tctccccagt atgtgatccg gcagatgacc ttcagggaga
      661 aaccctccgg gaaggacgga ggcaggatgc agaataccgg gaaaatccag gcaggaaata
      721 ctgaggaaat tcaggcagga gaatcgtaat gggaacatgg atctttccaa tggaaaatta
      781 tctggacttc agcaacagca ttttaccggt gctgatcgca ctgcttgtgt ttatggtgac
      841 ccggaagctt cgtcccggga agccgaccgt tatttccgtg ctgttcgggt ttcttttttc
      901 aatttgtatg gtatttggcg cacagcttga tcaaaagggc agtgttccat tcatgaatcc
      961 atggatgtgg ctttccgttc ttgcttttgc ggtagtgatg accctgatgg taagcggact
     1021 ctggagtgcg atggcacaga gacagcaatc ccgggtaact ttgccacatt tgaaagcctc
     1081 ccgggagatt tgcggagttt cgaaaacgca gacaggcagg acggaaggag gggcattttt
     1141 tctgctgcgg accggtggag tgatctttct tctttatttc gtggtatttc tggcagttta
     1201 tcccggattt ttcgtttatg atgcgcagga ggaatatctg gaagtggtaa cgaggagctt
     1261 taccacacat caccctctgt ttcatgtgct gatgctggga ggaattgtcc agcttgtgta
     1321 taaactgaca ggctctatca atctgggaat tgcagcctat accctgtttc agatggctgc
     1381 tttgtcactg attttcgggt attttatctg gaagctcggg gaacatgggc tccggaaacg
     1441 ggggcagtgg atccttaccc tttatcttgg catctgcccg ccgcttgtca tgttcagcct
     1501 gtgctcggca aaggacggcc tgttcatggg gatgctgctg atacaggtac tgcttttgct
     1561 ggatctgtgt gaggatgtgg atgcctttgg ggccgacagg aaaaaaatgc ttctgtttgc
     1621 ggtatctgcc atactgatga tgctgtttcg ccataacggc tgctacgcat ttctggtatt
     1681 ccttccnnnn nnnnnntttc tggctgcttt tgtaaaaaag aaagcgttct ctggcaatga
     1741 ccggatacag gcaggaagac gatctgccag gcttctggct tatggagtat tgctgatctg
     1801 cctgtatctg attttaaata aaggacttgc ttttgcaaca aaggcaagtg ggggtgagca
     1861 tcaggaaatg ctgacggttc ccattatgca gcttgcaaga acctatgcgt atgagccgga
     1921 tgcctttacg gaagaggacc gggaagtgct gttccggtat ctgccggagg atgccctgca
     1981 gcggtataca ccgaaggtat ccgacggggt gaaaatctcc ttttccaatg cagcctatga
     2041 agaaaactcc ggagatttct ggaagttgtg ggccgggatg ctgattcatc atccgttttc
     2101 ttatgtgaat gcatggctga tgacttcgta tggattctgg tatccggata cggtgattga
     2161 cgtgtatcgt ggaaacacgg tgt
//
LOCUS       DBMDQJ010000423         2179 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17400_length_2179_cov_1.426372, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000423 DBMDQJ010000000
VERSION     DBMDQJ010000423.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17400_length_2179_cov_1.426372"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="ACZO9R_08700"
     CDS             <1..682
                     /locus_tag="ACZO9R_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002567592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR0913176.1"
                     /translation="IHAQDTVMATGGIGGLYEHSTNFPILTGDACRIAEQHGVELEHM
                     DYVQIHPTCLYSKKPGRSFLISESARGEGAILLNHKGERFVNELLPRDVVTKAIHEEM
                     EKEGVDHEFISFENVPEEIIKGHFPHIYEKCLEEGYDILKEPAPIVPAQHYFMGGVHV
                     DHDSETTMPHLYAVGETSCNGVHGKNRLASNSLLESLVFAKRAAQKIELTQKGKEEHE
                     SNYHAACC"
     gene            651..1508
                     /gene="nadC"
                     /locus_tag="ACZO9R_08705"
     CDS             651..1508
                     /gene="nadC"
                     /locus_tag="ACZO9R_08705"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR0913177.1"
                     /translation="MNPITMQLVADKYIKMALEEDINSEDVSTNAVMPEYKKGDVQLI
                     CKEDGIIAGLQIFERVFTLLDEKTQVDFFVKDGEKVKKGQTLAVVTGDIRVLLSGERT
                     ALNYLQRLSGIATYTHSVAKLLEGSKTKLLDTRKTTPGMRIFEKYAVRIGGGCNHRYN
                     LSDGVLLKDNHIDAAGGVKEAVAAAKAYAPFVRKIEVETENLDMVKEAVEAGADIIML
                     DNMTPEQMAEAIRVIDGRAETECSGNITKENIKTITSLGVDYVSSGALTHSAPILDIS
                     LKHLRVLEG"
     gene            1517..2065
                     /locus_tag="ACZO9R_08710"
     CDS             1517..2065
                     /locus_tag="ACZO9R_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036094 - small molecule binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3H domain-containing protein"
                     /protein_id="HIR0913178.1"
                     /translation="MEKEMTGADRRKKLIFLMRESEQPLSGTQLGQVTGVSRQVVVQD
                     IALLRTEGYPIVSTAKGYLLNEPKCATRVLKVCHTNEQVEEELTTIVDLGGTVLNVMV
                     NHRVYGRVEAALNIRNRRDVQGFLNDLRTGKSVPLLNVTSGYHFHKISAESEEVLDEI
                     EHALQKKGFLAEVMPYEQETHA"
BASE COUNT          585 a          497 c          650 g          447 t
ORIGIN      
        1 catccatgcg caggatacgg tcatggcgac aggcggcatc ggcggtctgt atgagcactc
       61 cacgaatttc ccgattctga cgggcgatgc ctgccggatt gcggagcagc acggagtgga
      121 attggagcac atggattatg tgcagatcca tccgacctgt ctctacagca aaaagccggg
      181 cagaagcttt ctgatctcag agtccgccag aggcgagggc gcgattctct tgaatcacaa
      241 gggagagcgg ttcgtcaacg aactgcttcc gcgcgatgtc gtgacgaaag cgatccacga
      301 ggagatggaa aaagagggtg tcgaccatga attcatctcc tttgagaacg taccggagga
      361 gatcatcaag ggacatttcc cacatattta tgagaaatgt ctggaagagg gctatgacat
      421 cttaaaggag ccggcaccga tcgttccggc acagcactat tttatgggcg gcgtccatgt
      481 ggatcacgat tcagaaacga ctatgccgca tctgtatgcg gtcggggaga caagctgcaa
      541 cggggttcac gggaagaacc ggcttgccag caacagtctc ctggagagcc ttgtttttgc
      601 aaagcgcgcc gcacaaaaga tcgaacttac acagaaagga aaagaagaac atgaatccaa
      661 ttaccatgca gcttgttgct gacaaatata tcaagatggc gttagaggag gacatcaaca
      721 gtgaggatgt ctccaccaac gccgtgatgc cggagtacaa aaagggcgac gtacagttga
      781 tctgcaagga ggacggaatt attgcgggtt tacagatttt cgagcgtgtg ttcacactgc
      841 tggatgaaaa gacgcaggtg gatttcttcg tgaaggatgg cgagaaagtg aaaaagggac
      901 agacccttgc ggttgtgacc ggcgatatcc gggtactgct gtcgggagag cgtacggcac
      961 tcaactatct gcagcgctta agcggcattg caacttacac gcacagcgtg gcgaagcttc
     1021 tggagggatc taagacgaag cttctggata cccgcaaaac aacgccgggt atgcgtattt
     1081 ttgaaaaata tgcggtgcgc atcggcggcg gatgcaatca cagatataat ctgtcggacg
     1141 gcgtgctctt aaaggacaat cacatcgatg cggcaggcgg cgtgaaggaa gccgttgcgg
     1201 cagcgaaggc atatgcaccg tttgtgcgca agatcgaggt ggagacagag aaccttgata
     1261 tggtaaaaga agccgtggaa gcaggcgcgg atattatcat gctcgacaat atgacaccgg
     1321 agcagatggc ggaagcaatc cgcgtgatcg acggacgtgc cgagaccgag tgttccggca
     1381 atatcacaaa ggaaaatatt aagaccatta catctcttgg tgtggattac gtgtccagcg
     1441 gcgcgctgac acattctgcg ccgatcctgg atatcagtct gaaacatttg cgtgtgctgg
     1501 aagggtgaga aaatctatgg aaaaagaaat gaccggtgcg gacagaagaa aaaagctgat
     1561 ctttctgatg cgggagtcgg aacagccgct ctccgggacg cagcttggac aggtcacggg
     1621 ggtgagccgg caggtcgtgg tgcaggacat tgcgctgctt agaacagaag ggtatccgat
     1681 tgtgtcgacc gcgaagggct atctgctcaa cgaaccgaaa tgtgcgaccc gcgtgcttaa
     1741 ggtctgccat accaatgaac aggtggagga ggaactcacg acgatcgttg atctgggcgg
     1801 caccgtctta aatgtcatgg tgaaccacag ggtttacggg cgcgtggaag cggcgctcaa
     1861 tatcaggaac cggcgggatg tgcagggctt cttaaatgat cttagaaccg gaaaatcggt
     1921 tccgcttttg aatgtgactt ccggctatca ttttcacaag atttccgcgg aaagcgaaga
     1981 agtgctcgac gagatagagc atgcactgca gaaaaagggc tttctggcgg aagtgatgcc
     2041 ctacgagcag gagacacatg cctaaacggc acgatatgcc gcatgacacg ccgcatggca
     2101 tgccatgcgg cacctgtatg tcatagagaa ttgctgccat gtcgtaagga atcattttgc
     2161 agctcttgac ttcaaaccg
//
LOCUS       DBMDQJ010000424         2176 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17425_length_2176_cov_1.704096, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000424 DBMDQJ010000000
VERSION     DBMDQJ010000424.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17425_length_2176_cov_1.704096"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="ACZO9R_08715"
     CDS             <1..396
                     /locus_tag="ACZO9R_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913179.1"
                     /translation="LYWIVEWIAKIHSHILRLNDAYEYNFTDKELHFLVIGMMGMGFI
                     FVVYPVFKWLAKHDHVMVIAWIYVLTLIIVITFAIEIGQKVTGTGNMEFADIVMGVFG
                     FIVMFLVFSVVRGIYKLIRNLIRGDRKDE"
     gene            389..1264
                     /locus_tag="ACZO9R_08720"
     CDS             389..1264
                     /locus_tag="ACZO9R_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HIR0913180.1"
                     /translation="MNEFWKRVLETCVVILGNCLYAFGVAMFIVPGGLITGGTTGIAL
                     FVHHMMGCSVSVFVLLFNMVMFLIGFAVFGKKFAANTLVSTFSYPVALELFTRLLHGY
                     AITDDIILCMIFGGICIGGAIGVVIRAGASTGGMDIPPLVLNRYFKIPVSKSIYVFDM
                     LILLLQAFDSTGEQLLYGILLVIVYSVVLDKCLMLGTAKMQIKVVSEKIDEIRKAVLT
                     DIDRGVTMLKSETGYMERETEMLLTVVSIQELAKTEKLIHSVDEDAFVVINRVSEVSG
                     RGFSSKKRYLEKENS"
     gene            1261..2166
                     /locus_tag="ACZO9R_08725"
     CDS             1261..2166
                     /locus_tag="ACZO9R_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR0913181.1"
                     /translation="MTLQQLRYALTIAGCGSMNEAAKRLFLSQPSLSETVKELEQEIG
                     LQIFMRSNRGIIITPEGEEFLGYARQVIDQYGILKARYVEKQRKEKFSVSMQHYTFAV
                     KAFVETVKKAGMDSYEFAANETTTYDVMENVRNFKSEIGVLYLNDFNEQVLMKIIREK
                     GLEFEELFACDTYVYLWSGHPLAGQSVISMEELDEYPCLSFDQGRNSSLYLAEEMKST
                     YDYKRLIKANDRATLLNFMIGLNAYTLCSGIICEELNGSDYMAIPLKETEKMRIGYVK
                     RKGASISHIGQIYIEELKKYKEKIM"
BASE COUNT          573 a          405 c          632 g          566 t
ORIGIN      
        1 ctgtactgga ttgtggaatg gattgcgaag attcacagcc acattcttcg tctgaatgat
       61 gcgtatgaat acaattttac agataaagaa ctgcattttc tcgtcattgg tatgatggga
      121 atgggattca tttttgtcgt gtatcctgtt tttaaatggc tggcaaagca tgatcatgtc
      181 atggtaattg cgtggattta tgtgctgaca ctgattatcg tgatcacgtt tgccatagag
      241 atcgggcaga aagtaacagg caccggcaat atggaatttg cagacattgt gatgggtgta
      301 ttcggattca ttgtaatgtt ccttgtgttc tcggttgtgc ggggtattta taagctgata
      361 agaaatctga tcagaggaga caggaaagat gaatgaattt tggaaaagag tgctcgagac
      421 gtgcgtggtg attctgggga actgcctgta tgcattcggt gtggcaatgt tcattgtgcc
      481 gggaggactg attaccggcg gaacgacggg aattgcgctg tttgtgcacc acatgatggg
      541 ttgttccgtc tccgtgtttg tactgctgtt caatatggtt atgtttctga tcggatttgc
      601 ggtattcggg aaaaaatttg cagcaaacac actggtcagc accttcagct atccggtagc
      661 gctggagctg tttacacggc ttctgcacgg ctatgcgatc acggacgata taattctctg
      721 catgatcttt ggaggaatct gtatcggagg agccatcggc gttgtgatcc gtgcgggcgc
      781 atcgactggc ggcatggata taccgccgct tgtgttgaac cgttatttta agataccggt
      841 gtcaaagtcc atttatgtat tcgatatgct gattttgctg ctccaggcgt ttgacagtac
      901 aggagaacag cttctctatg gtattctgct ggtgattgtc tacagcgtgg tgctggataa
      961 gtgcctgatg cttggaactg caaagatgca gatcaaagta gtcagtgaga aaatcgatga
     1021 gatcagaaaa gcagtcttaa cagatatcga ccgcggtgtt acaatgttaa aaagtgagac
     1081 cggatacatg gagcgggaga cagagatgct gcttacggtg gtgtcaatac aggaactggc
     1141 gaagacggag aagctgatcc attcggtgga tgaggatgca tttgttgtga tcaatcgtgt
     1201 cagtgaggtt tccggccgcg gtttttcaag taaaaagcgt tatctggaaa aggagaattc
     1261 atgacattac agcaactgag atatgcactg acgatagccg gctgcggatc gatgaatgag
     1321 gcggcaaagc gtctgttttt atcgcagccc agtctgtccg agacggtaaa ggaactggag
     1381 caggagatcg ggctgcagat ttttatgcgc tcaaaccggg gaatcatcat cacaccggag
     1441 ggtgaggagt ttctggggta cgccagacag gtgatcgacc agtacggaat ccttaaggcg
     1501 cgctatgtgg agaagcagcg caaggaaaag ttcagtgtgt ccatgcagca ctatacgttt
     1561 gcggtcaaag cgtttgtgga gactgtaaag aaagcaggga tggacagcta tgaattcgcg
     1621 gcgaacgaga cgaccacata cgacgttatg gagaatgtac gcaactttaa gagcgagatc
     1681 ggcgttctgt atctgaacga ttttaatgag caggtgctga tgaagatcat ccgggagaag
     1741 gggttggagt tcgaggaact gtttgcttgc gatacctatg tctatctgtg gtcgggacat
     1801 ccgcttgcag ggcagagcgt gatctccatg gaagaactgg acgagtatcc gtgcctgtcc
     1861 tttgaccagg gacggaacag ctcgctctac ctcgcggaag agatgaagag cacctacgat
     1921 tataagcggc tgattaaggc gaacgaccgg gcaacactgc taaactttat gattgggctg
     1981 aacgcgtaca cgctgtgctc gggaattatc tgcgaggagc taaacggcag cgattacatg
     2041 gcaattccac tcaaggagac ggaaaagatg cggattggct atgtgaagcg caaaggggca
     2101 tcaatcagtc acatcggaca gatctatatc gaagaattga aaaaatataa agagaaaatt
     2161 atgtagtggt tggcta
//
LOCUS       DBMDQJ010000425         2174 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17450_length_2174_cov_1.244091, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000425 DBMDQJ010000000
VERSION     DBMDQJ010000425.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17450_length_2174_cov_1.244091"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1219)
                     /locus_tag="ACZO9R_08730"
     CDS             complement(<1..1219)
                     /locus_tag="ACZO9R_08730"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HIR0913182.1"
                     /translation="MELEKVKIHRIQQNGTAVSQITLDDDYNVPDYRPDIMKVLKENG
                     ELRFDEVKAANKAVWVKGSLVFHILYRCEQSDGKISCLKGEIPFQEKLNIDGLQENGE
                     VHAAGEIEDLTVGVINSRKLSIRAVVVLRASAEEQVLEEFTSRLELPGDYQQKTGTWG
                     ALNLLASCRDVCRQKSEIVLPSNKPNVREILWRSVELRNVESHVEDGKAVVTGEILAA
                     VLYSEEEESERLQWYETTVPLECAADCDAAGENCIFKISAVPLSAELEIKPDYDGEER
                     ILVLELSVSVDVRVWREEEMELLTDLYSLREKAVPVVRERIGERLLVKNAAKCRVTEQ
                     LEIPESQEKILQICACEGTVRLEKYEPVENGIRAEGTITAELLYITTDDNMPIQSARE
                     IYPFSQILEIPDMQ"
     assembly_gap    1233..1250
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1254..1496)
                     /locus_tag="ACZO9R_08735"
     CDS             complement(1254..1496)
                     /locus_tag="ACZO9R_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HIR0913183.1"
                     /translation="MQQADIHKVRASVYQQCGRKVLIQLDRGRNKVDIQEGVIQNAYP
                     SVFTILVNDEQEENPPQLLSFSYSDIITRDIRMKLC"
     gene            complement(1619..2044)
                     /locus_tag="ACZO9R_08740"
     CDS             complement(1619..2044)
                     /locus_tag="ACZO9R_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease complex subunit PrcB family protein"
                     /protein_id="HIR0913184.1"
                     /translation="MFWYGKRWKKGCLVFLAAAALLAAGCSIETTNGAKVADLDYEIV
                     EEAQIPEKVKAVIEEKKAADFKTTYELDGDLYIVRGYGEQETGGYSICIRDLYLTSNA
                     ILFDTELVGPRKGEQVSSGPSYPYIVIKTEKRDESTIFE"
BASE COUNT          465 a          662 c          418 g          611 t
ORIGIN      
        1 gctgcatatc gggaatctca aggatctggg aaaacggata gatttccctt gccgactgga
       61 tcggcatatt gtcatccgtt gtgatataga gaagttccgc cgtgatcgtt ccctccgccc
      121 ggattccgtt ctccacaggc tcgtattttt ccaggcgcac cgttccctcg caggcacaga
      181 tctgcaggat tttctcctgg ctttccggaa tttccagctg ttccgttacc cggcattttg
      241 ccgcattttt tacaaggagt ctctcaccga tccgctccct gactaccgga acggcttttt
      301 cccggagcga gtagagatcg gtcagaagtt ccatctcttc ttcccgccac acgcggacat
      361 cgaccgagac ggagagttcg agcacaagaa tgcgctcctc cccgtcgtaa tccggcttga
      421 tctccagttc cgccgacagc gggacagccg atattttaaa aatgcaattc tccccggcag
      481 cgtcacagtc tgccgcacat tccagaggaa ccgtcgtctc ataccactgc aggcgctcgc
      541 tctcctcttc ctcgctgtag agcaccgccg ccagaatctc acctgtgacg accgccttcc
      601 catcctccac atggctttcg acgttgcgga gttctacact gcgccacaga atttcccgca
      661 cattgggttt gttcgacggc agaacaatct cactcttctg ccggcagaca tcccggcagg
      721 atgcgagcag attgagcgct ccccaggttc ccgtcttctg ctggtaatcc cccggcagtt
      781 ccagtctgga cgtaaactcc tccaacacct gttcctccgc cgaggcacgc agcacaacca
      841 ccgcacggat gctcaatttt ctcgaattga ttacgccgac tgtcagatcc tcgatctctc
      901 cggcggcatg tacctccccg ttctcctgca gcccgtctat attgagtttc tcctgaaacg
      961 gaatctctcc tttgaggcag ctgatttttc catcactctg ctcacaccgg taaaggatgt
     1021 gaaacaccag gcttcctttt acccacaccg ccttgtttgc cgccttcacc tcgtcaaacc
     1081 gcagttcgcc attttccttc aaaaccttca tgatgtccgg ccggtagtcc gggacattat
     1141 agtcatcatc cagcgtgatc tggctgaccg ccgtgccatt ctgctgtatt ctatgtatct
     1201 ttactttttc gagttccaaa aaaatatccc ccnnnnnnnn nnnnnnnnnn atgttaacat
     1261 aatttcatcc gaatatctct tgtaatgata tccgaataac tgaatgaaag taactgaggc
     1321 ggattttctt cctgctcgtc attcaccagt attgtaaaaa cactcggata tgcgttctga
     1381 atgactcctt cctggatgtc gaccttgttg cgtcctctgt caagctgaat cagtaccttc
     1441 cgtccgcact gctgatacac ggaagcgcgt accttatgga tgtctgcctg ttgcattgtc
     1501 tacctccctg ttccggcttc ttatcgtcct aacaccgaat accattccgc tttagtttat
     1561 cactgtacta aatttttgtc aaacaaaccg gagaattttt taaaaatatc aagaaaattc
     1621 actcaaatat ggtactttcg tcacgttttt cggtctttat tacaatataa ggataactcg
     1681 gtccgctgct tacctgttct ccctttctcg gtcccacaag ttccgtatcg aacaaaatcg
     1741 cattcgacgt cagatacaga tcccggatgc agatgctgta acctcccgtc tcctgttctc
     1801 catacccgcg cactatgtac agatccccgt cgagttcata ggtcgtttta aaatcagccg
     1861 cctttttttc ctctatcaca gccttcactt tctccggtat ctgcgcctcc tccacgatct
     1921 catagtcaag atcggcaacc ttcgctccgt ttgtcgtctc aatgctgcac ccggcagcaa
     1981 gcagggccgc cgctgccaaa aagaccagac accccttctt ccatctcttc ccataccaaa
     2041 acatctgaca gtcccccaaa ttttcggttc tatccaaagt atatgccgcg tcttttttaa
     2101 atatgcctgt gaattctctt ttccgcgtct tgtgtttcct cttttcaggc tttataataa
     2161 aaactatgta aaaa
//
LOCUS       DBMDQJ010000426         2173 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17462_length_2173_cov_1.306467, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000426 DBMDQJ010000000
VERSION     DBMDQJ010000426.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17462_length_2173_cov_1.306467"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..848)
                     /locus_tag="ACZO9R_08745"
     CDS             complement(<1..848)
                     /locus_tag="ACZO9R_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR0913185.1"
                     /translation="MKFKNKIIISFCIIIFIPILLAVAVLFGFQKIQLKSIEQTYGVE
                     SDDYSYFSNSMQLLSRFTRESFETLLDLSKQDPDKLEDKAFLEQMNDTLREKYSYLVV
                     RRGDELIYVGDDKEASLLTDLPAYGGEESGADTGIYVDGDKQALIKQIDFVYTDGSQG
                     SVFIITILEETVPELKSLMADVVISIVLILILTACMLTVWIYTSFINPIKKLQVAAQN
                     IKDGNLDFTVDVETNDEIGELCRNFEEMRQRLKDNAEEKLSGERENKALISNIAHDLK
                     TPITAV"
     gene            complement(982..2163)
                     /gene="metK"
                     /locus_tag="ACZO9R_08750"
     CDS             complement(982..2163)
                     /gene="metK"
                     /locus_tag="ACZO9R_08750"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR0913186.1"
                     /translation="MEKRLFTSESVTEGHPDKVCDAISDAILDACMAEDPMSRVACET
                     ASCTGFVLVTGEITTKAKLDIPAIVRQTVNEIGYNDAKTGFDGNTCAVMVALDQQSPD
                     IAMGVDKALEAKEGGLKDELDTGAGDQGMMFGYATNETPELMPYPISLAHKLALQLTR
                     VRKDGTLSYLRPDGKTQVSVEYDENGKPLRLEAVVLSTQHDDDVTQEQIHEDIKKYVF
                     DPILPKELVDAQTKFFINPTGRFVIGGPHGDAGLTGRKIIVDTYGGYARHGGGAFSGK
                     DCTKVDRSAAYAARYVAKNIVAAGLAEKCEIQLSYAIGVAQPTSVMVDTFGTGKLSDE
                     KLVEIIRENFDLRPAGIIKMLDLRRPIYRQTAAYGHFGRADLNLPWEAVDKVETLKKY
                     L"
BASE COUNT          517 a          624 c          447 g          585 t
ORIGIN      
        1 ttcacggctg tgatcggcgt ctttaaatca tgggcgatat tactgatcag cgccttgttc
       61 tcacgctcac cggaaagctt ttcctctgcg ttatccttca gccgctgccg catctcctcg
      121 aaattgcggc agagctcccc gatctcgtca ttcgtctcta catccaccgt aaaatccaga
      181 ttaccgtcct taatgttctg cgccgccacc tgcagcttct tgatcgggtt gataaaactc
      241 gtataaatcc ataccgtcag catgcatgcc gtcaggatta agatcaggac aatcgagatc
      301 accacatccg ccatcaggct cttcaactcc ggcacggtct cctcaaggat cgtgataata
      361 aaaacgcttc cctggcttcc gtccgtatac acaaaatcaa tctgcttgat cagcgcctgc
      421 ttgtctccgt cgacatagat gccggtgtct gccccggact cttcccctcc gtaggccggc
      481 agatccgtca gcagcgacgc ctccttatca tctcccacat agatcagctc gtctcccctg
      541 cgcacgacaa gatacgaata cttctcgcgc agagtgtcat tcatctgctc cagaaacgcc
      601 ttatcctcca gcttatccgg atcctgcttt gaaagatcca gaagcgtctc aaaggactcg
      661 cgcgtaaaac ggctgagcag ctgcatggaa ttagaaaaat aactgtaatc gtcactctcc
      721 acgccgtatg tctgttcaat cgacttcagc tggatcttct ggaatccgaa aagcaccgcc
      781 accgcaagaa gaatggggat aaatatgata atacaaaatg aaatgataat tttatttttg
      841 aacttcatgt ctgctttgtc caccccgcaa attcccgcaa aaaaacgggt catagaactg
      901 taattctatg acccattata acacgaaaca tccaaaaacc tatttatttt atgtaaaatt
      961 gtctaaactt ttcataaaac tctacaaata tttctttaat gtctccacct tatcgactgc
     1021 ctcccacgga aggttcagat cggcgcgtcc gaaatgtccg tatgccgctg tctgacgata
     1081 aatcggtctt cttaaatcca gcattttgat gattccggcc ggacggagat cgaagttttc
     1141 acgaatgatt tccaccagtt tctcgtcaga aagctttccg gttccgaagg tatctaccat
     1201 aaccgatgtc ggctgtgcaa caccgatcgc ataggaaagc tgaatctcgc atttttctgc
     1261 aagacctgcg gcaacgatgt tctttgccac ataacgtgcc gcgtaagctg cggagcggtc
     1321 taccttggtg cagtccttac cggaaaaagc gccgccgccg tgacgtgcat atccgccgta
     1381 ggtgtctacg ataatcttac gcccagtcag acctgcatct ccgtgcggtc cgccgataac
     1441 aaaacgtccg gtcgggttga tgaagaactt tgtctgtgca tctaccagtt ccttcggcag
     1501 gatcggatca aacacgtatt tcttgatatc ctcatggatc tgctcctgtg taacatcatc
     1561 atcatgctgt gtggacagta cgacagcttc cagacgaagc ggctttccat tctcatcata
     1621 ctcaacggaa acctgtgtct ttccgtccgg tctcagatag gaaagtgtgc cgtccttgcg
     1681 caccctggtc agctgtaatg ccagcttgtg tgcgagagaa atcggatacg gcataagctc
     1741 cggtgtctcg tttgtcgcat aaccgaacat cataccctga tctcctgcac cggtatccag
     1801 ttcatccttt aacccgccct cttttgcctc gagtgccttg tctacaccca tggcaatatc
     1861 cggtgactgc tgatcgagcg ctaccataac tgcacatgta tttccgtcaa atccggtctt
     1921 cgcatcgttg taaccgatct cgttgactgt ctgtctcacg atcgccggaa tatccagctt
     1981 tgcctttgtg gtaatctctc cggttaccag tacaaatccg gtacagcttg ccgtctcaca
     2041 agccacacgg ctcattggat cctctgccat gcacgcatcc agaatcgcgt cggaaattgc
     2101 atcgcagact ttgtccgggt gtccttctgt gacggactca gatgtaaata atctcttttc
     2161 cattccttat tac
//
LOCUS       DBMDQJ010000427         2162 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17554_length_2162_cov_2.079573, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000427 DBMDQJ010000000
VERSION     DBMDQJ010000427.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17554_length_2162_cov_2.079573"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            209..>2162
                     /gene="pflB"
                     /locus_tag="ACZO9R_08755"
     CDS             209..>2162
                     /gene="pflB"
                     /locus_tag="ACZO9R_08755"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002576688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HIR0913187.1"
                     /translation="MQNEWRGFQRGSWEEEINVRDFIQKNYTPYDGDDSFLEGPTQDT
                     TDLWNEVLELSKQEREAGGVLDMDTKIISTITSHGPGYLDKNKEKIVGFQTDKPFKRS
                     LQPYGGIRMAVKACEDNGYHVDPEVVEYFTTHRKTHNAGVFDAYTPEMRACRSAHIIT
                     GLPDAYGRGRIIGDYRRPALYGVDRLIEDKEEQLATTRTIMYSDVIREREELSEQIRA
                     LKMLKELAKIYGCDISKPANNVLEATQAVYFSYLAAVKEQNGAAMSLGRTSTFLDIYA
                     ERDLREGTFTEKEIQEIIDQFVMKLRCVKFARTPEYNSIFAGDPTWVTESIGGIGVDG
                     RHMVTKMSYRYLNTLNNIGAAPEPNMTVLWSVQLPENFKRFCAQTSIRHSAIQYENDD
                     IMRVVHGDDYGIACCVSSMRIGKEMQFFGARANLAKCLLYAINGGVDEISGKQVGPKY
                     RPITSEYLDYDEVWDKYKDMMKWLAGVYVNALNIIHYMHDKYCYEKLEMALHDKKVRR
                     WFATGIAGFSVVADSLSAIKYAKVKPIRDENGITVDFEVEGDFPKYGNDDDRVDDIAK
                     EVLHTFIGYVKGNHTYRGGIPTTSILTITSNVSYGKNTGATPDGRKKGVAFAPGANPM
                     HGRDTNGAVASLASVAKMPFMDSQDGI"
BASE COUNT          596 a          511 c          605 g          450 t
ORIGIN      
        1 aaagatgctt tggttcaaaa agcataaaat acccactgag agtaagacaa gacaaacatg
       61 cctggcaaca aaacttgata ttgacaggga aaagcgtgct gtgctatagt cataaaggtt
      121 agctgcaact aactaatatc atgttttgac aatgctgtct gcgggcaggg aacataatca
      181 cattacaaag taagcaggag gaagaatcat gcagaatgaa tggagaggct ttcagcgggg
      241 aagctgggag gaagagatca atgtcagaga ttttatccag aaaaactata cgccttacga
      301 cggggacgac agctttctgg aaggaccgac gcaggatacg acagatttat ggaacgaagt
      361 gttagaactg tcgaagcagg agcgtgaggc aggcggtgtc ctcgatatgg ataccaagat
      421 tatctccacg atcacatccc acggaccggg ttatctggac aagaataagg agaagattgt
      481 cggctttcag acagataagc cgttcaaacg ttctctgcag ccgtatggcg gaatccgtat
      541 ggcagtgaaa gcctgcgagg ataacggcta tcatgtagat ccggaagtgg tggaatattt
      601 taccacacac agaaagacac acaatgctgg tgtatttgat gcctacacac cggagatgcg
      661 cgcatgccgt tcggcacaca tcatcacggg acttccggat gcatatggcc gcggacgtat
      721 tatcggcgat tacagaagac cggcactcta cggtgtggat cgcctgatcg aggacaagga
      781 agaacagctc gcaacgaccc gtacgatcat gtactcggat gtcatccgtg agcgtgagga
      841 gctttccgag cagatccgcg cactcaagat gctgaaagag cttgcaaaga tttacggctg
      901 tgacatttcc aagccggcga acaacgtgct ggaagcgacg caggcggtat atttttccta
      961 ccttgcagca gtcaaggagc agaacggtgc ggcaatgagc cttggacgta cctccacatt
     1021 cctcgacatc tatgcggagc gcgatctcag ggaaggaacg ttcacagaga aggagatcca
     1081 ggagatcatc gaccagtttg tcatgaagct gcgctgcgtg aagtttgcac gtacgccgga
     1141 gtacaactcc atcttcgctg gggatccgac ctgggtgacg gagtcgatcg gcggaatcgg
     1201 tgtggacggc agacatatgg tgacgaagat gtcctaccgc tacttaaata cactcaacaa
     1261 catcggagca gcgccggagc cgaatatgac cgtgctctgg tcggtacagc ttccagagaa
     1321 ctttaagcgt ttctgcgcac agacatccat caggcactct gcgatccagt acgagaatga
     1381 tgacatcatg cgcgtggtac acggggatga ctacggaatt gcatgctgtg tatcctctat
     1441 gcgcatcggc aaggagatgc agttctttgg cgcccgcgct aaccttgcaa agtgccttct
     1501 gtacgcgatc aacggcggtg tagatgagat cagcggcaag caggtcggac cgaagtaccg
     1561 cccgatcact tccgagtacc tggattacga cgaggtgtgg gataagtata aggatatgat
     1621 gaagtggctg gcaggtgttt atgtcaatgc cttaaacatc attcattata tgcatgacaa
     1681 atactgctac gagaagctgg agatggcgct tcatgacaag aaggtgcgcc gctggttcgc
     1741 gaccggaatc gctggttttt ccgttgtggc ggattccctg tctgccatca agtacgcaaa
     1801 ggtaaagccg atccgcgatg agaatggaat caccgtagac tttgaggtgg agggcgactt
     1861 tccgaaatac ggaaatgatg acgaccgcgt cgacgacatt gcaaaggaag tgcttcacac
     1921 attcatcggc tatgtcaagg gcaaccacac ctaccgcggc ggaatcccga cgacatcgat
     1981 cctcacgatc acatccaacg tatcctacgg caagaacaca ggtgcgacac cggatggcag
     2041 aaagaagggc gttgcctttg caccgggcgc aaacccgatg cacggacgcg acacgaacgg
     2101 tgcggtagca tctctcgcgt cggtagcgaa aatgccgttt atggattccc aggatggtat
     2161 ct
//
LOCUS       DBMDQJ010000428         2160 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17586_length_2160_cov_1.518698, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000428 DBMDQJ010000000
VERSION     DBMDQJ010000428.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17586_length_2160_cov_1.518698"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="ACZO9R_08760"
     CDS             complement(<1..218)
                     /locus_tag="ACZO9R_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR0913188.1"
                     /translation="MQQSSGAVRTRIVLDAMGGDYAPAEPVKGAVDAVNARSDIQVLL
                     VGQEDVVREELAKYTYPGDQIQVVHAEEV"
     gene            complement(321..1043)
                     /locus_tag="ACZO9R_08765"
     CDS             complement(321..1043)
                     /locus_tag="ACZO9R_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HIR0913189.1"
                     /translation="MGDFFNLDNKFFQGLSKVVDCVILSVLWVFCCIPMATTFFLAWQ
                     SASVFGWILCWLTSFLAGPATTALYYAVNKVIRHGRSYLWKEYWHAFKSNFKQAALAA
                     LILTGVVLFLGLDCYIMYQFAAQGQKSGVLYVVFLVLIVLVVMLAVYLFAYMARFENS
                     LGQSFKNAALIAVANLPWSLLLLVLLAASAFGLWMLPWLMILLPAVYILVANFILERI
                     FRKYMSAEDLEAEQERNQEFFN"
     gene            complement(1065..1955)
                     /locus_tag="ACZO9R_08770"
     CDS             complement(1065..1955)
                     /locus_tag="ACZO9R_08770"
                     /inference="COORDINATES: protein motif:HMM:NF024758.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR0913190.1"
                     /translation="MKAVLMLWGIGLLLGLGGCAEQTQDAAGEESRTTEQIPQSTEQM
                     PQDSGQTGAETEQMAPGSGQTGTGTEQAVQSSEGTGVETDGAEDTMEAVLEQVLPCVV
                     QIYHGTPEGGHTAGSGFIMEMTDDTVYLCTNRHVIAKYDDWDVYFYDGTCVTGSKAGT
                     DDVYDVGVVAVDRTAIPKEVQEKLKTVSYDLSSWEELGNAELPVGIVRIGQEGNVLHT
                     LTGSLLRKETEFLWGQGEKETEVRMAITDGDSGSAVFDENGKLVSMIFGCSQDAGGER
                     DWGVPLRAIVSCYEKITGET"
BASE COUNT          505 a          711 c          401 g          543 t
ORIGIN      
        1 acctcttctg catgtaccac ctgaatctga tctccggggt acgtatactt tgccagttct
       61 tcccgaacga catcctcctg tcctaccagg agcacctgaa tatccgaccg tgcgttcacg
      121 gcatccactg cgcccttgac cggctccgcc ggtgcgtagt ctccgcccat cgcatccaat
      181 acgattcttg tcctcacagc cccgctgctc tgctgcatac tccatctccc atctgtcctg
      241 ccggagtcta gttctccacg cctactaatg aatatactat aactctttct ataaatcaac
      301 aataaaaaat acgcttattt ctaattgaaa aattcctgat ttcgttcctg ctccgcctcc
      361 agatcttccg ccgacatata cttgcggaaa atgcgctcca ggataaaatt cgccaccagg
      421 atgtagactg ccggaagcag tatcataagc cacggaagca tccacaagcc aaaggcgctc
      481 gctgccagaa gcaccagaag aagcagcgac cacggaagat tcgccaccgc aatcagcgcc
      541 gcgttcttaa acgactgccc cagactgttc tcaaagcgtg ccatgtaggc aaacagatat
      601 accgccagca tcacgacaag cacaatcagt accaggaaaa ccacatacag cacgccgctc
      661 ttttgtccct gcgcagcaaa ctgatacata atatagcagt ccagtcccag aaacagaacc
      721 actcctgtca gaatcagcgc tgcgagcgcc gcctgcttaa agttgctctt aaacgcatgc
      781 cagtattcct tccacagata gcttcttccg tgccggatta ccttattgac cgcataatag
      841 agcgccgtcg ttgccggtcc cgccagaaac gatgtcagcc agcacagaat ccatccgaat
      901 actgatgcgc tctgccatgc caggaaaaat gtcgttgcca tcgggatgca gcagaacacc
      961 cacagcacgc ttaagatcac acagtcaacc accttactga gtccctgaaa aaacttattg
     1021 tccaaattaa aaaaatcacc catatgaatc cttctttctg tctcctatgt ttctcctgtt
     1081 attttctcat aacatgacac gatcgcccga agcgggacgc cccagtcacg ctcgcctccg
     1141 gcgtcctgtg aacatccgaa tatcatgctg acaagttttc cgttttcatc aaaaaccgcc
     1201 gatccggaat ccccgtcggt aatcgccatg cgcacttccg tctccttctc tccctgtccc
     1261 cacaggaatt ccgtctcttt ccgaagaaga cttcccgtca gcgtgtggag cacattgcct
     1321 tcctgtccga tgcgcacgat tccgaccggc aactctgcat tgccaagttc ttcccagctc
     1381 gaaagatcat acgacaccgt ctttagcttt tcctgcactt cctttggaat cgccgtccgg
     1441 tcaaccgcca cgacgcccac atcataaaca tcgtcggtac ccgctttgct tcccgtaacg
     1501 caggttccat cataaaaata cacatcccag tcgtcgtact ttgctatgac atggcgattc
     1561 gtacacagat acaccgtatc atccgtcatt tccataataa aaccggaacc cgcggtatgt
     1621 cctccctccg gcgtcccgtg ataaatctgt accacacacg gcagcacctg ctccaacacc
     1681 gcttccatag tgtcttccgc gccgtccgtc tccaccccgg ttccttcact gctctgtacc
     1741 gcttgttccg ttcccgtccc ggtctgcccg ctgcccggtg ccatttgttc tgtttccgct
     1801 ccagtctgtc cgctgtcttg cggcatctgt tccgtgctct gcggtatctg ctccgttgtc
     1861 ctgctctctt ccccggcagc atcctgtgtc tgttctgcac agcctcccag tcccagtaaa
     1921 agccctatac cccaaagcat cagcactgcc ttcatcctgt atctatgcac tctacgcatt
     1981 tcttcttcaa tccgtttcca ccagtttttc tcttgtacca tattcattta ttatagcttt
     2041 ccataacgcc ccggtcaagg aacaaaaggc acagcacaaa gtgttttcct ataaagaaaa
     2101 aacagccgcg tctccgcggc tgtttttccc taaagaatca ttttagtaat tgtactcgga
//
LOCUS       DBMDQJ010000429         2157 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17613_length_2157_cov_1.357490, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000429 DBMDQJ010000000
VERSION     DBMDQJ010000429.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17613_length_2157_cov_1.357490"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1584
                     /locus_tag="ACZO9R_08775"
     CDS             <1..1584
                     /locus_tag="ACZO9R_08775"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III family protein"
                     /protein_id="HIR0913191.1"
                     /translation="QSLRLLKLFGNTTSKKVTPSVGPEQEYFIVDREKYLQRKDLIFT
                     GRTLFGAMPPKGQEMDDHYFGSIRERIGAYMKDVNEELWKLGVTAKTQHNEVAPAQHE
                     LAPIYAEANVAVDHNQLIMETLKKVAGRHGLQCLLHEKPFAGVNGSGKHNNWSITTDD
                     GINLLEPGKTPHENIQFLLVLTCILKAVDRHADLLREAAADVGNDHRLGANEAPPAIV
                     SIYLGEQLQDVLSQLISTGEATHSISGKKLETGVKTLPDFMKDATDRNRTSPFAFTGN
                     KFEFRMVGSQDSIAQSNVVLNTIVAEAFAEACDVLEKADDFDMAVHDLIKEYATEHQR
                     IVFNGNGYSDEWVAEAEKRGLPNICSMVDAIPALNTEKAVTLFEKFKVFTKAELDSRV
                     EIEYETYAKEINIEAKAMIDIAAKQIIPAVIKYTTVLAQSINAVKSACGADISAQTEI
                     LTEVSDLLADAQNALDKLKDVTEKASAMDEGREQAVYYRDTVKTAMDELRAPVDKLEM
                     LVDKSMWPMPSYGDLIFEV"
     gene            1874..>2157
                     /locus_tag="ACZO9R_08780"
     CDS             1874..>2157
                     /locus_tag="ACZO9R_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR0913192.1"
                     /translation="MKKLEIIIKPEKLEDLKGVLDTEEVNGLNIVNSMGYGNQKGIIK
                     NYRGAEYSVNLLPKIKVETVVADEVAPKLIDKIVDEINTGHFGDGKIFVY"
BASE COUNT          667 a          448 c          572 g          470 t
ORIGIN      
        1 cagtcacttc gcttattaaa attattcgga aatactacat caaagaaagt aacaccgtcc
       61 gttggaccgg agcaggaata ctttatcgta gaccgcgaga agtatttaca gagaaaggat
      121 ctcatcttta ccggacgtac cttattcgga gcaatgccgc cgaagggaca ggagatggac
      181 gatcactatt tcggatcgat tcgtgagcgc atcggtgcgt acatgaagga cgtcaatgag
      241 gaactctgga agcttggcgt tacggcaaag acacagcaca acgaggtggc tccggcacag
      301 catgagcttg ctccgatcta cgcagaggcg aacgtggcag tcgatcacaa ccagctcatt
      361 atggagacat tgaagaaagt tgccggccgt catggtttac agtgtctgct tcatgagaag
      421 ccattcgcag gtgtgaacgg ttccggtaag cacaacaact ggtcgatcac gacagacgat
      481 ggcatcaacc tcttagagcc gggcaagaca ccgcatgaga acatccagtt cctgttagtc
      541 cttacctgca tcttaaaagc agtagatagg catgcagatc tcttgagaga ggcagcagca
      601 gatgtcggaa acgatcacag attaggagca aacgaggcac cgccagcaat cgtatccata
      661 tatcttggtg agcagctgca ggatgtgctt tcacagctga tcagcactgg tgaggcaaca
      721 cacagtatct ccggcaagaa gttagagacc ggtgttaaga cgcttccgga tttcatgaag
      781 gatgcaaccg accgtaaccg tacatcgccg tttgcattca caggcaataa attcgagttc
      841 cgtatggtag gttcccagga ttccatcgcc cagtcgaacg tggtactcaa cacgatcgtg
      901 gcagaggcat ttgcagaggc atgcgacgtc ttagagaagg cagatgactt tgacatggca
      961 gtacatgatc tgatcaagga gtatgcaacc gagcatcaga gaatcgtatt caacggcaac
     1021 ggatattcag acgagtgggt agcagaggca gagaagcgcg gtcttccgaa catctgctcc
     1081 atggtagatg cgatcccggc actcaacaca gagaaggcag tgacactgtt tgagaagttc
     1141 aaggtattta ccaaggctga gctggattcc cgtgtggaga tcgagtatga gacctacgca
     1201 aaagagatca atatcgaggc aaaggcaatg atcgacattg ctgcaaagca gatcattccg
     1261 gcagttatca aatatacaac ggtactggca cagtccatca atgccgtaaa gagtgcatgc
     1321 ggcgcagata tcagcgcgca gacagagatc ctcacagagg tatccgatct tctggcagac
     1381 gcacagaatg ctctcgacaa gttaaaggat gtgacagaga aagcatctgc aatggatgag
     1441 ggcagagagc aggctgtata ctacagagat acggtaaaga cagcgatgga tgaactcaga
     1501 gcgccggtag ataagctcga gatgttagtt gataagtcaa tgtggccgat gccgtcctat
     1561 ggagatttga ttttcgaggt ataactgcta ccataaacct tctaaaaaaa aatcccccct
     1621 aaagggagaa aaaactcccc gaaaggggag gattgccagg tgacaaccgt cacctggcag
     1681 attatgaaaa agttttgtgg gtagtaacaa atactgataa ctatcatttg tatggttata
     1741 gtatatggag cagaaatgaa aatttcatgt ttacaggatg aaagattttt gaatcttaaa
     1801 tgaataaact gtgaacagca gagggatggg agcagcgctt tcgctaaaac tgaatataaa
     1861 ggaggagctt ggcatgaaaa aactggaaat tattattaag ccggaaaaac tggaagactt
     1921 aaagggcgtg ctggataccg aggaagtaaa cggtttgaat atcgtcaaca gcatgggcta
     1981 tggtaaccag aagggaatta ttaagaacta ccgcggtgca gagtattccg tgaatctgct
     2041 tccgaagatc aaagtcgaga cggttgtggc agacgaagtt gcaccgaagc tgattgacaa
     2101 gatcgtagat gagattaaca ccggacattt cggagatggt aagatcttcg tgtatga
//
LOCUS       DBMDQJ010000430         2156 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17624_length_2156_cov_1.423358, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000430 DBMDQJ010000000
VERSION     DBMDQJ010000430.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2156)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17624_length_2156_cov_1.423358"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            165..1487
                     /locus_tag="ACZO9R_08785"
     CDS             165..1487
                     /locus_tag="ACZO9R_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913193.1"
                     /translation="MNNDVFETMPVPKAYFKMALPVVFGMVISLVYNMVDTWFIAKTQ
                     NTSLVAGVSLCAPVFSLMIAFGDIFGLGGSSAISRLFGKKDYDTARRISAFCLLGSIF
                     FGIAVAVLMLLFQTPILHLLGARDDTIAYAGAYYRFLVLGCPAIIFNIVPGNLLRTEG
                     LANDAMIGSVIGAVFNILLDPIFIFSLHMGAGGAALATILSNLLADCYLVWVVFKKAG
                     HLSMSCREMHISSGHVRDILLIGIPASITNLMQSFAVLLTNRFLLPYGSDKVAALGIA
                     LKVNMITMLILVGFAFGAQPLLGYCYGADNRERLKQFLHFDLLVQLLIALVFTAAACI
                     FAPHIIRIFMQDDVIVASGALMLRCLMITAPVIGMILVFTTLFQAAGMALPAFLMSIS
                     RQGVLLVLCMLLFSSLFGYMGILLAQAVSDVLTGILGFLLLRRSKILL"
     gene            complement(1968..>2156)
                     /locus_tag="ACZO9R_08790"
     CDS             complement(1968..>2156)
                     /locus_tag="ACZO9R_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006906797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR0913194.1"
                     /translation="HAFGMVKVGEKGQIVIPAKARKIFDIKPGDVLMVLGDEEQGIAL
                     LKEQDFMAMVQAMRHMET"
BASE COUNT          438 a          625 c          463 g          630 t
ORIGIN      
        1 tacagtgtac tccttttgct caacgatttc tcatttattt gtcagtagtt tactgcacta
       61 atgtggcaat gtcaagaagc tttttatttt cccgtcacct tttttctatc aatatgatat
      121 actttaagca ttgatcctgt tacaggaaat ggaggaactc tcagatgaac aatgatgtat
      181 ttgaaacaat gcccgtaccg aaagcctatt tcaagatggc tctgcctgtg gtgttcggca
      241 tggtcatcag ccttgtatac aatatggtcg acacatggtt tattgcaaag acccagaaca
      301 ccagcctcgt cgccggcgtc tctctctgcg ccccggtgtt cagtctcatg attgcattcg
      361 gtgatatttt cgggcttggc ggaagttccg ccatctcaag acttttcgga aaaaaagatt
      421 atgacaccgc gcggcggatc agtgctttct gccttctcgg ctccatcttc ttcggcattg
      481 ctgttgccgt tctgatgctg ctttttcaga cacccattct gcatcttctg ggtgccaggg
      541 acgataccat cgcttatgcc ggcgcctatt accgctttct ggttctcggc tgccctgcca
      601 ttatctttaa tattgtcccg gggaatctgc tccgcacaga aggtctggca aatgatgcca
      661 tgattggttc cgtcatcggc gctgtgttta atattctttt agatcccatt ttcatcttct
      721 ctctgcatat gggtgccggc ggcgccgcac ttgccaccat cctgagcaat ctgcttgccg
      781 actgctatct cgtgtgggtt gtattcaaaa aggcagggca tctgtcgatg tcctgccgcg
      841 aaatgcatat ttcatccggt catgtccgtg acattctgct gatcggaatt ccggcttcca
      901 tcacgaacct gatgcagagc tttgcggtac tcctcaccaa ccgttttctg ttaccttacg
      961 gttctgataa agttgccgct cttggcattg ccttaaaagt caacatgatc accatgctga
     1021 ttctggtcgg gttcgccttc ggcgcgcagc cgctcctcgg ctactgctac ggtgcagaca
     1081 accgcgaacg cctgaaacag tttctgcatt ttgatctgct cgtgcagctt ctcattgcgc
     1141 tcgttttcac agccgccgcc tgtatttttg ctccgcacat catccgcatc ttcatgcagg
     1201 atgatgtcat cgtggcctcc ggtgccctga tgctccgttg tctcatgatt accgcccctg
     1261 tgatcggcat gatcttagtc ttcaccacac tgttccaggc agccggcatg gcgcttcccg
     1321 cttttctgat gtccatcagc cgccagggcg tgctgctcgt gttgtgtatg ctgctctttt
     1381 cttccctgtt cggctacatg ggaattctgc tcgcgcaggc agtctccgat gtcctcaccg
     1441 gaattctggg ctttcttctg ctgcgcagat caaaaatact gctctgatgc gccgtgcgtc
     1501 acaccgtctc tgcgctgcgg taaacctgct caaacgtatc tcttgcagta ttatgcttcc
     1561 cgatgtcaac aaccaccgca ccgatggaca tggaagactt tacgtgatcc gggagcaggg
     1621 caatcttctc cttgtacagc gcgcaacttt ctttgcgtac ttctgaatct ctgcttcctt
     1681 gatcggcacc cgttgcccgt gtccgcgcag aatctcatcg aattccatat agcgcgcaca
     1741 gcatccagct cttccgtggt aaacaacaga tacatggaga acaccgaaaa tgccgactca
     1801 aaccagtaaa tgatttttgg cagcgcaatg cggcagaact ccgcaaaaaa ccaagcacat
     1861 aaatcaccga cgccagcgca aataaaatac ttccgacctg cgctctggaa ggtctcgcct
     1921 gtgttatttt acatattgca atcctccgcg caccggattg cgcaatttta cgtctccata
     1981 tgccgcattg cctgcaccat cgccataaag tcctgctcct ttaacagcgc gattccctgt
     2041 tcttcatctc ctaaaaccat cagtacatcc cccggcttta tgtcaaaaat tttcctcgcc
     2101 tttgccggga tcactatctg ccctttttca ccgaccttca ccattccaaa cgcatg
//
LOCUS       DBMDQJ010000431         2152 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17674_length_2152_cov_1.305705, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000431 DBMDQJ010000000
VERSION     DBMDQJ010000431.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17674_length_2152_cov_1.305705"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1700
                     /locus_tag="ACZO9R_08795"
     CDS             <1..1700
                     /locus_tag="ACZO9R_08795"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR0913195.1"
                     /translation="KIGQLFFNMGSSRDEEYLKMTVDKYHIGGIRYNPATGAEVREQN
                     RILQENSKIPLIIACNTENGGNGACTDGTMIGQQTKIGATRDARYAYELGRMSNKEAA
                     AIGCNLSFAPVSDILYNWENPVIGLRTYGNDVDRVCEMTKAYMDGAHTNEGFCCAAKH
                     FPGDGLDFRDQHVADSVNDMSCEEWDASYGKVYQNLIDNGLEAIMAGHIMQPAYTRHF
                     NPGIKDEDIMPATLSPELIEGLLRGKLGFNGMVLTDASHMVGLTCCMKRSEMLPHTIA
                     AGVDMFLFFNEMDEDFASMMAGYENGIITEERLNDALHRILALKAHMGLHKKAKNEIV
                     PPVEVMEQVVGCEEHKAMAREISEKGITLVKYKDEDVLPMIPSRYKRIMIVSVSGLSA
                     GVMGTMMAKYMGGGKKSPAERLRDKLIEKGFDAFIYESPLDALAKRAAAGEKVDINMY
                     FAGKTPIKEFVENQDLIITLVDIAGGFQPVARPGFGMSKGGGEIPWYVHELPVIVIGC
                     GQPFVLADIPQARTYINTYDSADQTFDALIEKLMSGSDAFIGEDPVDAYCGLWDTHR"
     gene            1863..2102
                     /locus_tag="ACZO9R_08800"
     CDS             1863..2102
                     /locus_tag="ACZO9R_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016441137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3791 domain-containing protein"
                     /protein_id="HIR0913196.1"
                     /translation="MMADEKIKNSSELEFVVFCIENVAAKLGMDAERVYQAFAEKSDI
                     LSGYIVPEYEVLHTQSREYIVDDLIDVMKERGVEV"
BASE COUNT          573 a          476 c          641 g          462 t
ORIGIN      
        1 agaagatcgg tcagcttttt ttcaatatgg gatcctccag agacgaagag tatctgaaga
       61 tgacagtgga caagtaccac atcggcggta tccgctacaa cccggcaacc ggtgccgagg
      121 tgcgtgagca gaaccggatt cttcaggaga acagcaagat tccgcttatc attgcgtgca
      181 acacggagaa tggcggcaac ggcgcctgca ctgacggaac catgatcggc cagcagacca
      241 agatcggtgc gacaagagac gccagatacg cgtacgagct cggccgtatg tccaacaaag
      301 aggcggcggc gatcgggtgt aatctctcct ttgccccggt cagcgacatc ctctacaact
      361 gggagaaccc ggtcatcgga ctgcgcacct atggaaacga tgtggaccgc gtgtgcgaga
      421 tgacgaaggc atatatggac ggcgcacaca ccaatgaggg attctgctgt gctgcgaagc
      481 attttccggg cgacggactg gacttccgcg accagcacgt ggcagacagc gtcaatgaca
      541 tgagctgtga ggagtgggat gcaagctatg gaaaagtata ccagaacctc attgacaacg
      601 gtctggaagc catcatggca ggacacatta tgcagcctgc gtacaccaga cattttaacc
      661 cgggaatcaa ggatgaggac atcatgccgg caaccctttc gccggagctg atcgagggac
      721 ttctgcgcgg aaagcttggt tttaatggta tggtgctgac cgatgcttcc cacatggtcg
      781 gactgacctg ctgtatgaag cgcagcgaga tgcttccgca cacgattgca gccggtgtcg
      841 atatgttcct ttttttcaat gagatggatg aggattttgc aagcatgatg gcgggctatg
      901 agaacggcat catcaccgag gagcgtttaa atgacgccct gcaccgcatt cttgctctga
      961 aagcacacat gggactgcac aaaaaagcga aaaatgagat cgttcctccg gtggaggtga
     1021 tggagcaggt cgttggctgc gaagagcaca aggcgatggc aagagaaatc tccgagaaag
     1081 gaatcactct cgtaaaatac aaggacgagg atgtgcttcc gatgatcccg agccgttaca
     1141 agcgcatcat gatcgtctcc gtcagcggat tatccgcggg cgtgatggga accatgatgg
     1201 caaaatatat gggcggcggc aagaagagtc cggcagagcg cctgcgtgac aagctgatcg
     1261 agaagggctt tgacgcattt atctacgaga gcccgctcga cgcacttgca aagcgcgcgg
     1321 cagcaggaga aaaggtggac atcaatatgt actttgccgg aaagacgccg atcaaggagt
     1381 tcgtcgagaa ccaggatctg attatcacac tggtggacat tgcaggtggt ttccagccgg
     1441 tggcaagacc gggcttcggc atgagcaagg gcggcggaga gatcccgtgg tatgttcatg
     1501 aacttccggt tatcgtgatc ggatgcggac agccgttcgt actggcagac attccgcagg
     1561 cgagaacgta tatcaacact tacgacagcg cggatcagac gtttgatgcg ctcatcgaga
     1621 agctgatgag cggcagcgac gcatttatcg gggaggaccc ggtggacgcc tactgcggac
     1681 tgtgggatac gcaccggtag gggcttacgc aaaaggatca ggatatacag gcacttgggt
     1741 cgtaatgacc cgggtgcctt ttttagaggt aatttgattg agcatatgga tatgaagtgc
     1801 tatactaaca gtgataagaa gagaaggctg ttcttcatat tggcataaag gagagcttgc
     1861 ttatgatggc ggatgaaaaa attaaaaatt ccagtgaact cgagtttgtt gtattttgta
     1921 tcgaaaatgt agcggcaaaa ctggggatgg atgcagagcg tgtgtatcag gcgtttgccg
     1981 aaaaaagtga tattctaagt ggttatattg tgccggaata tgaagtgctg catacacaga
     2041 gccgggaata tatcgtagat gatcttattg atgtgatgaa agaaaggggc gtggaagtat
     2101 gattctctac catggttctt ttggaaaaga cgcgacagat tatgatctcg tt
//
LOCUS       DBMDQJ010000432         2148 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17716_length_2148_cov_1.645823, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000432 DBMDQJ010000000
VERSION     DBMDQJ010000432.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17716_length_2148_cov_1.645823"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(33..1145)
                     /locus_tag="ACZO9R_08805"
     CDS             complement(33..1145)
                     /locus_tag="ACZO9R_08805"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004848955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR0913197.1"
                     /translation="MSRILVTRSSMPEYEEYIEEIKDIWDSHWLTNMGAKHNELEKEL
                     EKYLKVDHVSLFSNGHMALELLIQALHLTGEVITTPFTFASTTHAIVRNGLTPVFCDV
                     NPEDYTMDVSKIEALITDKTSAIIPVHVYGNLCDVEAIEAIAKKHHLKVIYDAAHTFG
                     VTYKGRGVATFGDASMFSFHATKVFHTIEGGAVCFNGEQNGLKEDLYGLKNFGIRNEV
                     VVDAVGANSKMNEFQAAMGLCNLRHLDGEIEKRSRVVARYREHLSGVPGIKLVQPQKD
                     VKPNYAYFPVVFDETVLGYGRDDIYEMLKQHDIFTRKYFYPLTSEFQCYSDRYDAKDT
                     PVALETSKRVLTLPLYADLDLSDVDRICELILGYRA"
     gene            complement(1295..>2148)
                     /locus_tag="ACZO9R_08810"
     CDS             complement(1295..>2148)
                     /locus_tag="ACZO9R_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0913198.1"
                     /translation="GVYAMNDRVVCVMSVGAGKYRKQYELVRDNLMAYAKKCHADFRF
                     IDDYIDKDKKRDIYSQKLLIPDYLREYEQVLFLDLDIIISPDCPDIFALMPENIGLMA
                     EVNPRSSARFRKIYADNERILNETVEDYFTSRGFAAADGLVGNINGGVLLFRPRLVAD
                     LFRDYYMSMKSQGENTAFEEAPMAYYTQTKGLFLELDYRFNCMPYFEIGNPYADRLYA
                     LHQNRLYGKAEHVLSRLTGEKHMLLLGAHYKLIRRLFDDGAYIVHFAGGYWNTHLYRK
                     CMKHLWR"
BASE COUNT          503 a          635 c          456 g          554 t
ORIGIN      
        1 gttgcccatg ccgtcaccga tttttacaat aatcatgccc gataccccag aatcagctca
       61 cagatgcggt ccacatccga aagatccaga tccgcataga gcggcagtgt cagcacacgc
      121 ttcgacgtct caagcgccac cggcgtatcc ttcgcatcat atctgtcact gtaacattga
      181 aattcgcttg tcagcggata gaaatacttt ctggtgaaaa tatcatgctg ctttaacatc
      241 tcataaatat catccctgcc atatccgagc actgtctcat cgaacaccac cgggaaatat
      301 gcatagttcg gctttacatc cttctgcggc tgtaccagct tgattcccgg cacaccgctt
      361 aaatgctctc tgtagcgcgc caccacgcgg ctgcgcttct cgatctctcc atccagatgc
      421 cgcaggttgc aaagtcccat cgccgcctgg aattcattca tcttggagtt ggcgccgacc
      481 gcgtccacaa cgacctcatt gcggattccg aaattcttaa gaccgtacag atcctccttc
      541 agtccgttct gctctccgtt aaagcataca gcgccgccct cgatcgtgtg gaacaccttc
      601 gttgcgtgga agctgaacat ggacgcatcc ccgaacgtcg ccacaccgcg tcccttgtac
      661 gtaacgccaa acgtatgcgc cgcatcgtaa atcactttca gatgatgctt tttggcgatt
      721 gcctcgatcg cctccacatc acagagattg ccgtagacgt gcaccggtat gatcgcgctg
      781 gtcttgtccg tgataagcgc ctcgatcttg gagacatcca tcgtgtagtc ctccggattc
      841 acatcacaga ataccggtgt cagcccgttc cgcacaatcg catgtgttgt ggacgcaaac
      901 gtaaacggcg tggtaatgac ctctcccgtc agatgcagtg cctgaatcag aagctccagc
      961 gccatgtgtc cattggaaaa aagggaaacg tgatccacct tcagatactt ttccagctct
     1021 ttttccagtt cattgtgctt cgcacccata ttcgtcagcc agtggctgtc ccagatatcc
     1081 ttaatctctt ctatatattc ttcatactcc ggcatggaag aacgtgttac caatattctg
     1141 ctcattttct cgcctttcct ctcccgcagc tctctgcggg ggcagtccta tcctgcattt
     1201 tcagaaatag ataccctgtc atgataataa aaatgcaact tcttgtcaat ctttgcgcca
     1261 ttttccgcgc atttctaatg atttcttaag aaattcagcg ccacagatgt ttcatacact
     1321 tcctatataa atgtgtgttc cagtatccgc ccgcaaagtg cacgatgtag gcgccgtcgt
     1381 caaacaggcg gcggatcagc ttgtagtgcg caccgagaag cagcatatgc ttttctcctg
     1441 taagccggct taggacatgc tcggcttttc cgtacagccg gttctgatga agtgcataca
     1501 gcctgtccgc atacggattg ccgatctcaa aatacggcat gcagttaaac cggtaatcca
     1561 gttccagaaa cagccccttc gtctgggtgt aatacgccat cggcgcttcc tcaaacgccg
     1621 tgttctcgcc ctggctcttc atcgacatgt agtaatcccg gaacaggtcg gcaacaaggc
     1681 gcggacggaa caataacacg ccaccgttga tattgccgac cagaccatcc gccgctgcaa
     1741 acccgcgcga cgtaaaataa tcctccaccg tctcgtttaa aatgcgctcg ttgtccgcat
     1801 agattttgcg gaaacgcgcg gacgagcgcg gattgacctc cgccatcagt ccgatattct
     1861 ccggcataag cgcaaaaata tccgggcaat ccggcgagat gatgatatcc agatccagga
     1921 aaagcacctg ctcgtattcc cgcagatagt ccggtatcag aagcttctgt gaatagatgt
     1981 ccctcttttt gtccttgtcg atatagtcgt ctataaagcg aaaatccgca tggcacttct
     2041 tcgcataagc catcagattg tcccgcacca gttcgtattg ttttctatat tttcctgcgc
     2101 caacagacat tacgcatacc actctgtcat tcattgcgta gaccccct
//
LOCUS       DBMDQJ010000433         2148 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17722_length_2148_cov_1.225208, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000433 DBMDQJ010000000
VERSION     DBMDQJ010000433.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17722_length_2148_cov_1.225208"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            58..816
                     /locus_tag="ACZO9R_08815"
     CDS             58..816
                     /locus_tag="ACZO9R_08815"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0913199.1"
                     /translation="MKMNEKLLPEFYRLSHSFNQMLDRLNQAFAAQRQFTGNAAHELC
                     TPLALMQAQLELFSAEHPDINAETAEFLRLLGEQTERLTQMSKTLLEMSSLQQVARND
                     QIHLAPMIEEIFTDLAPLAEKRGITLEQDGDGVMTGSDALIYRLLFNLTENAIKYNRT
                     NGSVRVCVTREDTNIHIYVTDTGCGIPAEFQRSIFQPFFRVDKSRSREYGGAGLGLSL
                     VWEIADLHGGSVRVAESSKQGTTIAVELPALSYS"
     gene            complement(902..1324)
                     /locus_tag="ACZO9R_08820"
     CDS             complement(902..1324)
                     /locus_tag="ACZO9R_08820"
                     /inference="COORDINATES: protein motif:HMM:NF024110.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0913200.1"
                     /translation="MSTAPIRVSDIVLGKFYALATLLFIPVAIISIYPLVLSAFGEIS
                     LAETYLAVGAFFLYGLAYITIGEFVSSITESQMIAAIISFGILFAGYVMNAMPKLYMV
                     TGHGELELDSAFTDILQKANIDTQTDIILRSMTAAVFT"
     gene            1651..>2148
                     /locus_tag="ACZO9R_08825"
     CDS             1651..>2148
                     /locus_tag="ACZO9R_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016286776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913201.1"
                     /translation="MKQNSYELKQHILQVWKLSLPAILTQITTIVMQYIDSAMVGALG
                     ANASASIGLVSTTTWLFGGVTYAVSAGFSVQVAHHIGAGEEQEARNVERHGLLTALCL
                     SGILCLTGILLSPHLPIWLGGSEDIIRNSSAYFLVFALMVPFSQLNSLSSSFLQCSGD
                     MVTPSI"
BASE COUNT          559 a          537 c          498 g          554 t
ORIGIN      
        1 cccttacgca gcttttctgc gcaggtagaa aaggtacaga tcaataatct tacagacatg
       61 aaaatgaacg aaaagcttct gccggagttt taccggctga gtcattcttt taatcagatg
      121 ctggaccggc tgaatcaggc ctttgcagcc cagcggcagt ttaccggtaa tgctgcccat
      181 gagctgtgta ctccccttgc gctcatgcag gcacagttgg agctgttttc cgcagagcat
      241 cccgatatca atgcggaaac agcagaattt ctccggcttt tgggagaaca gacggaacgt
      301 ctgacccaga tgtcaaagac acttctggaa atgagcagtc ttcaacaggt ggcacgtaat
      361 gatcagatcc atcttgctcc aatgattgag gaaatcttta cagatcttgc tccattggct
      421 gagaagcgcg gcatcacgct ggagcaggat ggcgatggtg tcatgactgg cagcgatgcc
      481 ttaatctacc ggctgctctt taacctgaca gaaaatgcca tcaagtacaa ccgcacgaac
      541 ggttccgtcc gggtttgtgt aacccgggag gatacgaata tccacattta tgtgaccgac
      601 accggatgtg gcattcctgc agaatttcag cggagtatct tccagccctt tttccgggta
      661 gacaaatccc gcagccggga atacggcggt gcaggactgg ggctttctct ggtatgggaa
      721 attgcagacc tccatggcgg atccgtccgg gtagcggaaa gctcaaagca gggcaccacc
      781 atcgcagtag agcttcccgc tctgagttat tcctgatctg cggcttccgt gctctcttcc
      841 tcatccgccg cttcaaaaca ggaagaatcc ttatgcagca gataataggt actactgtca
      901 atcaggtaaa cactgctgcc gtcattgacc gtaagataat atccgtctgc gtatcgatgt
      961 tcgccttttg caggatgtcc gtaaaggcag agtccagttc gagctcgcca tgtccggtca
     1021 ccatataaag cttaggcatt gcattcatta catagcctgc aaacagaatg ccaaaggaaa
     1081 taattgctgc gatcatctgg ctttccgtaa tggaggacac aaattctcca attgtaatat
     1141 aggcaagacc gtaaaggaaa aatgccccga ctgccagata agtttctgct aaagaaatct
     1201 caccaaacgc actcagaacc agaggataaa tactgataat cgcaaccggg atgaacagca
     1261 atgtggccag tgcataaaac ttacccagca caatatccga aacccggatc ggtgctgttg
     1321 acaaaaactg atctgaattc ctttttataa attgccagca tcttcctcac ctccctgaac
     1381 gtcttcctgt ttttccgact cctgcgataa aaaaacaaag attttgtgaa aattatatgt
     1441 tttcctattt gtaccgaccg gggaattgat taaaagacct ggaaagttac aggtatcccg
     1501 gcacggcttg acaggtctgt caggatatga tacggtcgaa gggcgatcct ccgtgaatcc
     1561 tctgttcaca atttccgtga cagcatggat accaggaaat aaccctatta acaaactgct
     1621 gtattgaaca gttaccttct tataatactt atgaaacaga actcttacga actgaaacaa
     1681 catattttac aggtatggaa gctgagcctt cctgccattc tgacacagat taccacaatt
     1741 gtgatgcaat acattgactc cgccatggta ggggcactgg gagccaatgc ctctgcttcc
     1801 atcggacttg tttccacaac gacgtggctc tttggaggcg ttacttacgc ggtttctgcc
     1861 ggattttccg tacaggtagc ccaccacatc ggtgccggtg aggaacagga agcccgtaat
     1921 gtggaacggc acggccttct cactgctttg tgtctgtcgg gaattttgtg tctgaccggg
     1981 attttactaa gtccgcacct ccccatctgg ctgggaggca gtgaggatat tatccggaat
     2041 tcctccgctt attttctggt atttgcactg atggttccct tttcgcagtt aaattcactg
     2101 tcctcctcat tcctgcaatg cagtggggat atggttaccc caagtatc
//
LOCUS       DBMDQJ010000434         2147 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17735_length_2147_cov_1.070381, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000434 DBMDQJ010000000
VERSION     DBMDQJ010000434.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17735_length_2147_cov_1.070381"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /gene="minD"
                     /locus_tag="ACZO9R_08830"
     CDS             <1..674
                     /gene="minD"
                     /locus_tag="ACZO9R_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HIR0913202.1"
                     /translation="NLDVVMGLENRILYNLVDVLTGRCRVKQALIRDKRYPNLWIIPS
                     SCSGDSKNNLNEKQMKNLLSDLRKEFDYVLIDSPAGIDGGFKLATADSDRIIVVTTPQ
                     IAAIHDADCVIQLLHRKKVPRIDLLVNGYRRQMVRDGNMLDIPDICELLEAELLGVVL
                     EDEEIIISQNHGEPVIGKHTVSDTCYQNIARRIAGMEVAIPDYLHHKGMFSRFFWRNH
                     AGKLV"
     gene            676..879
                     /gene="minE"
                     /locus_tag="ACZO9R_08835"
     CDS             676..879
                     /gene="minE"
                     /locus_tag="ACZO9R_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HIR0913203.1"
                     /translation="MCHRSKGVAKERLKLMIDSRRKQLDDETLAQIRHEIGEIVTRYV
                     DIEPENVEIKIILKDYICSNNTN"
     gene            858..>2147
                     /locus_tag="ACZO9R_08840"
     CDS             858..>2147
                     /locus_tag="ACZO9R_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007890038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR0913204.1"
                     /translation="MFKQYKLKNYKFILIALVVILNTIGVALVGSASPGDQKKQIIGM
                     VSGIIIMLLVSYIDYNFILRFSWLIYLGAIGLLGLVIVAGEQSKGAQRWFKIGGFQFQ
                     PSELVKILMILFLAYYFMRYEEKINSPRVLFGSFVLIGIPLALILAQPNLSTTIVLAL
                     VFAAMLFAGIFGGLASVIIKLSAGGVQSLLEGWHAPELLVSLICDVLFSVVYFAVMMA
                     SFVLGISLGFNLMEETGYLARISFLFDPTMSKVGLQGKTIMPFFMGLGCTIAGTTGTR
                     VVDNWGQRVLAITVSWAVPCGATLSVVSMLAMALFGNTGGFLVIVSIFVVMIMELPPY
                     HKPNVRNTLYVTFERTVDIFMRALRVITIVSVVFFVLTYGFGGSAETSILYQLGVIIE
                     PVTLFFGMKWEAFLAFLASAISKKSLLGVLNTLYGTGG"
BASE COUNT          498 a          473 c          591 g          585 t
ORIGIN      
        1 ggaacctcga tgtagtcatg ggactggaaa accggatcct ctataatctg gtcgacgtcc
       61 tgaccgggag atgccgcgtt aagcaggcgc tgatccggga caagcggtat ccgaatctct
      121 ggatcatacc gtcttcgtgc tccggtgact ccaaaaacaa tctgaatgaa aaacaaatga
      181 aaaatctgct gtcggacctg cgaaaagaat ttgattacgt tctgattgac agtccggcgg
      241 gaattgacgg cggttttaag ctggcgacgg cggattccga ccgcattatt gtggtcacaa
      301 cgccgcagat tgcggcgatt cacgatgcgg actgtgtaat ccagcttctg caccggaaaa
      361 aggtgccccg gatcgatctg ctggtcaacg gttaccgcag acagatggtg cgtgacggca
      421 acatgctcga tattccggat atctgtgagc ttctggaagc ggagcttctg ggtgttgttc
      481 tggaagacga ggagattatc atcagccaga accacgggga accggtgatc gggaagcata
      541 cggtttccga cacctgttac cagaatattg cacgccggat tgccggcatg gaagtcgcaa
      601 taccggatta tcttcatcac aaagggatgt tctccagatt cttctggcgg aatcatgccg
      661 gaaagctggt gtaggatgtg tcatagaagc aagggtgttg caaaagagcg gctgaagctg
      721 atgatcgaca gccgaaggaa acagctggat gacgagaccc tggcgcagat ccgccacgaa
      781 attggcgaaa tcgttaccag atatgtcgat attgaacccg aaaacgttga aattaaaatc
      841 atacttaagg attacatatg ttcaaacaat acaaactaaa gaattacaag ttcatattga
      901 ttgcactggt ggtgatctta aataccatcg gtgtggcgct ggtgggaagt gcaagcccgg
      961 gcgaccagaa aaagcagatc atcggtatgg tatccgggat catcattatg cttctggtat
     1021 cctatattga ttataatttt atactgcgtt tttcctggct gatctatctg ggcgccatcg
     1081 gtcttctggg gcttgtcatt gtggcaggag agcagagcaa gggtgcgcag cgctggttta
     1141 agatcggcgg atttcagttc cagccgtccg agctggtcaa gattctgatg atcctttttc
     1201 tggcttacta ttttatgcgg tacgaggaaa aaataaattc cccgcgggtg ctgttcggat
     1261 cttttgtgct gatcggcatt ccgcttgccc ttattctggc acagcccaat ctttccacga
     1321 ccatcgttct ggcgctcgtt tttgcggcga tgctgtttgc cgggatcttt ggcggattag
     1381 ccagcgtcat aataaaactg tcagccgggg gcgtacaaag tctgctcgag ggatggcacg
     1441 cgccggagct tctcgtctca ctgatctgtg acgttttatt tagcgttgtt tattttgcgg
     1501 tcatgatggc ttcgtttgta ctgggcatct cgcttgggtt taatttaatg gaagaaacgg
     1561 gctatctcgc gaggatctcc ttcctgtttg atcctaccat gtccaaagta ggtttacagg
     1621 gaaaaacgat catgcctttc tttatggggc ttggatgtac gatcgccgga acgaccggaa
     1681 ccagagtcgt tgacaactgg ggacagcgtg tgcttgcaat aaccgtgagc tgggcagttc
     1741 cctgcggtgc cacgttatcg gtcgtttcga tgcttgcgat ggcattgttt ggaaataccg
     1801 gaggctttct cgttatcgtt tccatttttg ttgtaatgat catggaactg ccgccgtatc
     1861 ataaaccgaa tgtcagaaat acgctttatg ttacatttga gcgaaccgtt gacattttca
     1921 tgcgggcact cagagtcatt acgatcgtgt cggtggtgtt ctttgttctg acctacggat
     1981 ttggcggcag cgcggaaacg agtattttgt atcaactcgg tgtcatcata gagccggtga
     2041 ctcttttctt cgggatgaag tgggaggcgt ttttggcatt cctggcatct gcgatttcta
     2101 agaaatcgct gctcggtgtg ctgaatacgt tatatggcac cggggga
//
LOCUS       DBMDQJ010000435         2144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17754_length_2144_cov_1.538913, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000435 DBMDQJ010000000
VERSION     DBMDQJ010000435.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17754_length_2144_cov_1.538913"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            13..1626
                     /locus_tag="ACZO9R_08845"
     CDS             13..1626
                     /locus_tag="ACZO9R_08845"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0913205.1"
                     /translation="MIRRLGRAQEGIHFLDILLDETICEGKILVPEELVRDAGGINEK
                     LQAKQKYELLLRIAQQSPIVLEEISGKESSDGYVILGADEADCSAEQGCVTDCYVIGK
                     YSGELKAAGCFDMAIMGVLEQAQKLGCEERMVPYLEHMVAHGKEYYALDEETEPILIY
                     KGDDVCHNVLTVFAEQFGAALEHAGQHVIYYDLNDEDVAGILKFMGRHFKAVIGVQSY
                     LFSVKMADEVHYVHEYIYGPKYNFIFDHPVWLQKHLQHHYPDFYVLTLDQNYADFAKR
                     YFGKKAILFPPAGMAAEEPYEPERKYGLTFVGTMGDYFSQVLWIHGLERPKRFLANRF
                     LLIMRQHPELTLEQAFARAVEHQGLKLTDQEFLDRLRELCVTGYCVMHYYRARVVETI
                     LKSGIRLDVFGNSWKLSPLCRYPNLICHPDVTVEESLTIYKQSQMSLNVMSWHKGGFT
                     ERMADIMLAGAVLVTDDTTYLRGRYNNDDMLIYHLDNMDQLPEQIAALLADEERRLQI
                     AKNGREKTVREHTWDGRAVQFLQILRERR"
     gene            complement(1610..>2144)
                     /locus_tag="ACZO9R_08850"
     CDS             complement(1610..>2144)
                     /locus_tag="ACZO9R_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR0913206.1"
                     /translation="PFTRSAGEFITPKDVYTGVIQKQVTSEERISLLNAITNVAPVAL
                     YSASDTSLCPKAAPMGIVSYTAEMPDIFHTTKINLNITLRSITSGIPLRAIDILGCGG
                     FLLSNYQPELCEYFTPDVDFVYFENPDDLAEKVTYYLSHEKEREDIAYHGYQTVTQNF
                     SYPVQVSRMFELFTSAL"
BASE COUNT          589 a          407 c          619 g          529 t
ORIGIN      
        1 cggagatgcg gaatgatacg gagattagga cgcgcgcagg agggaatcca tttcctggat
       61 atcctgttgg atgaaacaat ctgtgagggt aagatcctgg taccagaaga actggtgcgc
      121 gatgccgggg gaatcaatga aaaacttcag gcaaagcaaa aatatgaatt actgcttcgg
      181 attgcacagc agtcaccgat tgtgctggaa gaaattagtg gtaaggaatc atcggacgga
      241 tatgtaatac ttggcgcgga tgaagcggat tgctctgcgg agcagggctg cgtgacagac
      301 tgctatgtaa tcggaaaata cagtggcgaa ctcaaggcgg cgggctgctt tgatatggca
      361 attatgggtg tactggaaca ggcgcaaaag ctggggtgcg aagagcggat ggtaccttat
      421 ctggaacata tggtagcaca cggaaaggaa tattatgcgc tggatgagga aacggaaccg
      481 attttgattt ataaagggga tgatgtgtgc cataatgtgc tgacagtgtt tgcggagcag
      541 tttggtgcgg cgttggaaca tgcgggacaa catgtcattt attatgattt aaatgatgag
      601 gatgtcgcgg gaattttaaa atttatggga agacatttca aggcggttat cggtgtacaa
      661 tcttatttgt tctccgtaaa aatggctgat gaagtgcatt atgtacacga atacatttac
      721 ggccccaaat ataactttat atttgaccat ccggtatggc tgcagaagca tttacagcat
      781 cactatccgg atttttatgt tcttacgctg gatcagaatt atgcagattt tgcaaaaaga
      841 tatttcggaa aaaaggcaat tctgtttcct cctgctggaa tggcagcaga agaaccgtac
      901 gagccggaac gtaaatacgg actcacgttt gtgggcacga tgggagacta tttttctcag
      961 gtattgtgga ttcacgggct ggagcgtccg aagcgttttc tggcaaaccg tttccttctg
     1021 attatgaggc agcatcccga actgacatta gaacaggcat ttgccagagc ggtggaacat
     1081 cagggactga aactgaccga tcaggaattc ctggaccggc tcagagaact ctgcgtcaca
     1141 ggatattgtg tgatgcatta ttatcgcgcc agagtggtgg agacgatctt aaagagcggg
     1201 atccggctgg atgtattcgg aaacagctgg aagctgtcac cgctttgcag atacccgaat
     1261 ctgatctgtc atccggatgt cacggtggag gagagcctga caatctacaa acagtcccag
     1321 atgtccttga atgtaatgtc atggcataag ggcggattta cagagagaat ggcagatatt
     1381 atgctggcgg gagcggtact ggtcacggat gacactacat atctgcgcgg acgttacaat
     1441 aatgatgata tgttgattta tcatctggat aatatggatc agctgccgga gcagattgcg
     1501 gcgctgcttg cggatgagga gcgccggctg cagattgcga aaaatggaag agaaaagacg
     1561 gtacgggagc atacctggga cgggcgagct gtgcagtttt tgcagattct cagagagcgg
     1621 aggtaaaaag ttcaaacatg cgggatacct gaacgggata ggaaaaattc tgcgtgaccg
     1681 tctggtagcc atggtatgcg atatcttccc gttctttttc gtgcgagaga tagtaggtga
     1741 ctttttctgc cagatcatcc ggattctcaa aatatacgaa gtccacatcg ggtgtaaaat
     1801 attcacagag ctccggctga tagttgctta acagaaagcc gccacagccg agaatatcga
     1861 tggcgcgaag cggaataccg gacgtgatcg accggagagt aatattgaga ttgatcttgg
     1921 tggtatgaaa gatatccggc atttctgctg tgtaagatac aattcccatg ggcgctgcct
     1981 tggggcataa cgatgtatct gaagccgagt acagtgctac cggagcgaca ttggtgatgg
     2041 cgttgagcag agagatccgc tcttccgagg taacctgctt ctggataacg ccggtataca
     2101 catctttggg agtgataaat tccccggcag agcgggtaaa ggga
//
LOCUS       DBMDQJ010000436         2144 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17760_length_2144_cov_1.286344, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000436 DBMDQJ010000000
VERSION     DBMDQJ010000436.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17760_length_2144_cov_1.286344"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="ACZO9R_08855"
     CDS             <1..942
                     /locus_tag="ACZO9R_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913207.1"
                     /translation="LRAMMENLQQTQQVLETNTGNLNQLMKQNSAYAEDNSAATQELA
                     AGMEETSANAAHIVENVGIMRESSDNIQRLAEDGEKNSGQIQERAGEMERISTESRHK
                     TDQMYAVMKQKTDAAVEQAKSVQKINALTDNIKQISSQTNLLALNANIEAARAGEAGR
                     GFAVVASEIGDLATQTLDTVSTIDEIVGEVNSSVSNMTECLTTIMEFLEQTVLGDYEH
                     FAQVGEQYHADADTFQQIMQQTKEAVDALEQHIGEISSTVSEINSMVEQSTDGISGIA
                     EKSGSTQNLVTEGYDKLQECTQSVNVIRDFVAQFHLD"
     gene            1045..1890
                     /locus_tag="ACZO9R_08860"
     CDS             1045..1890
                     /locus_tag="ACZO9R_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR0913208.1"
                     /translation="MKKILKPETTKPQYVVTTDITYAQVDSWFGHCRRDLKLDLIYPE
                     DGEGKTYPCIVWICGGAWQRMDKAAHLAYLGTLAQEGFVVASVEYRTSNEGTHPMQLC
                     DIKAAIRYLRAYAKRYRINSEKFGVMGESAGGYLTCMAALDQDKKLDTGEYLEYSSQV
                     QAACPWYPPTDASHFPYDDVEKAAMSSESLLMGFNVMTHPQEAYENSPVSKVTPDAPP
                     FLLIHGTKDSTVPFSQSEELYDALEAAGCDVTLLALDGAEHADLMFFQDEVWQQIIAF
                     FKRTL"
BASE COUNT          651 a          423 c          588 g          482 t
ORIGIN      
        1 ctccgggcga tgatggaaaa cctgcagcag acgcagcagg ttttggaaac aaataccgga
       61 aatttaaacc agcttatgaa acagaacagc gcttatgcgg aggacaattc ggcggctacg
      121 caggaactgg cagccgggat ggaagagacc agtgcaaatg cggcgcacat tgtggaaaac
      181 gttggaatta tgagagaaag ttccgacaat atccagcggc ttgcagagga tggagagaaa
      241 aattccggac agattcagga gcgtgccgga gagatggagc ggatcagtac ggagtcccgc
      301 cacaagacag accagatgta tgcggtcatg aaacagaaga cggatgccgc tgtggagcag
      361 gcgaaatccg tacagaagat caatgcgctg acagacaaca tcaaacagat ttcttcccag
      421 acaaaccttc tggcattaaa tgccaatatt gaggcagcca gggctggtga agcgggcagg
      481 ggatttgcgg ttgttgcttc cgaaatcgga gatctggcaa cgcagacact ggataccgtt
      541 tccacaattg acgagattgt aggagaggtc aacagttcgg tttccaatat gaccgaatgc
      601 ctcacaacga ttatggaatt tctggaacag accgttcttg gggattatga gcatttcgca
      661 caggtggggg agcagtatca tgccgatgcg gacacattcc agcagatcat gcagcagaca
      721 aaagaagcag tggatgcatt agagcagcac attggagaga tctcttctac cgtatcagag
      781 atcaactcca tggtggaaca gtccacagat ggaatcagcg gtattgccga gaaatccggc
      841 agtacgcaga accttgtaac cgagggttac gacaagctgc aggagtgcac acagtccgtc
      901 aatgtgatcc gggattttgt cgcacagttt catttggatt gataattgtc cgaagataag
      961 ttaagacgtg gttagtatag actaatatga aaccctttaa atataggaaa aggaaatttt
     1021 acatcggaaa cgaggaaaat tataatgaag aaaatattaa aaccggaaac cacaaaacca
     1081 caatatgttg tgacaacaga cattacttat gcgcaggttg attcctggtt cggtcattgc
     1141 cggagagatc tcaaactgga tctcatctat ccggaggacg gggaaggaaa gacatatcca
     1201 tgtattgtat ggatttgcgg tggcgcatgg cagcgcatgg acaaagcagc gcatctggca
     1261 tatctgggca cactggcgca ggaagggttt gttgttgcca gcgtagagta ccggacatcc
     1321 aacgagggaa cgcatccgat gcagctgtgt gatatcaagg cagcaatccg atatcttcgt
     1381 gcatatgcaa aaagatatcg gattaacagt gaaaaatttg gtgtgatggg agaatctgca
     1441 ggaggatatc tgacatgtat ggcagcactg gatcaggata aaaaactgga tacaggtgag
     1501 tatctggagt attccagtca ggtacaggcg gcctgtccgt ggtatccgcc gaccgatgcg
     1561 tcccattttc catatgatga tgtggaaaag gcagccatgt cttcagagtc gctgctgatg
     1621 ggatttaatg ttatgacaca tccgcaggaa gcttatgaga attccccggt ttccaaagtg
     1681 acaccggatg cgccgccatt cctgctgatt catggaacca aagacagtac cgtgcctttt
     1741 tcgcagagtg aggaattgta tgatgcgtta gaagcagccg gctgtgatgt gacattgctt
     1801 gcgcttgatg gcgcagagca tgcggacctg atgtttttcc aggatgaagt atggcagcag
     1861 atcattgcat tttttaaacg gacattataa taagtgatct ggtatccgga taaggaaagg
     1921 ttcaaagatt aattgatatg aaaaaacagc agataacatt cgtataatag gaaaatccga
     1981 tggcccgaca tccgttttta ttgccggaaa agacaaaaag aaaacgttaa gacagaagat
     2041 agaaaagagc atgtatgact tccggcgaaa gcgtgtaatc aaatttctga gggcagattc
     2101 gcactctgtg gatgaggtgg cagattatgt ggtcagggaa ctgg
//
LOCUS       DBMDQJ010000437         2136 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17844_length_2136_cov_1.199509, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000437 DBMDQJ010000000
VERSION     DBMDQJ010000437.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17844_length_2136_cov_1.199509"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(13..1323)
                     /locus_tag="ACZO9R_08865"
     CDS             complement(13..1323)
                     /locus_tag="ACZO9R_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913209.1"
                     /translation="MEDTKKNEMWGKVMAPLAGFLAIVLVAFLIILKLLESGVIGTVM
                     AMILICVIVAAVFGGIFMVIKSILDQIRSVINGKVSENTKVAEKAKKLADREDGIGEM
                     VRSIETSVSSIELVLGGIRKAVDELEGVSGDFQQIFLEMSESMDQTADAVDTITGNTV
                     SQASETADMKEKVDAISVSIDHISSNIEDLTKSAQAMREYNRSAEQIMQELIAISEES
                     GVAIENVRKQTDLTNQSAQQIRTATEIIAGISSQTNLLALNASIEAARAGEHGKGFAV
                     VAEEIRTLADQSRESTEQINHVVNVLLDNSSNSVQITEQVSEAFSRQNEKIHDTEEIF
                     KSLNREIEQVGDAIGGIGTEVGDLENHKSVIENGVTSLTASADENAKSAEVTTDNMGR
                     LRGVVKECDGVKAHIVSVSDELIGYIRKFSDGSILDKFGAGKNR"
     gene            complement(1404..>2136)
                     /locus_tag="ACZO9R_08870"
     CDS             complement(1404..>2136)
                     /locus_tag="ACZO9R_08870"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR0913210.1"
                     /translation="YKKKVTAVIGGGDVQNAYNAGSTIGSYLAALGFNVDFAPVADVL
                     TNPDNQVLGQRSFGSDAQTVAGMVTSELQGLSAAGVYGTVKHFPGHGGTSGDSHDGAV
                     STDKTLEELMAEELVPFQSAIDGGVNFVMVGHISAPNVTGDNAPATLSKVLITDVLRG
                     QMGYNGIVITDAMNMEAITGFYNSDKAAVLAVTAGADMILMPADYNTAYTGILNAVND
                     GTITEERINESVTRIVKAKLAMGQP"
BASE COUNT          457 a          682 c          451 g          546 t
ORIGIN      
        1 cctgctaaat gtttatctgt ttttacccgc accgaacttg tcgagaatcg aaccgtccga
       61 aaacttacgg atgtatccaa tcagctcgtc cgatacgctg acgatgtgcg cttttacgcc
      121 atcgcactcc ttcaccacgc cgcggagacg tcccatatta tctgtggtga cctctgcgct
      181 cttcgcattc tcatctgccg aagccgtaag ggaagtgacc ccgttctcaa tgacgctctt
      241 gtgattctca agatcgccga cctccgtgcc gattccgccg atagcatctc cgacctgttc
      301 gatttctcgg ttcaatgatt tgaagatctc ctccgtgtca tgaatcttct cattctgtct
      361 ggaaaatgcc tccgatacct gctcggtaat ctgaacgctg ttgcttgaat tatccagcag
      421 gacattcacg acatggttaa tctgctctgt ggactctctg gactgatccg ccagtgtccg
      481 gatctcctcc gcgaccacag cgaatccctt accgtgctcc cctgctctgg cagcctcgat
      541 gctcgcatta agggccagaa ggttggtctg gcttgaaatt cccgcaatga tctcagtcgc
      601 cgtccggatc tgctgcgcgg actgattcgt cagatctgtc tgctttctga cattctcgat
      661 cgcaacgccg ctctcctcac tgatggcaat cagctcctgc ataatctgct ccgccgaacg
      721 gttatattca cgcatggcct gcgcactctt cgtcagatcc tcaatattgg aagaaatatg
      781 atcgatcgac acgctgatcg cgtccacctt ttccttcata tccgccgtct cagacgcctg
      841 ggacaccgta ttgccggtga tcgtatcgac agcatctgcc gtctgatcca tcgactccga
      901 catttccaag aaaatctgct ggaaatcacc ggatacccct tcgagctcat ccacagcctt
      961 gcggattcct ccaagcacca gctcaatcga tgaaacggac gtctcgatgg aacgcaccat
     1021 ctccccgatg ccgtcttcac ggtctgccag ctttttcgcc ttttccgcaa cttttgtatt
     1081 ctccgatacc tttccgttaa tcacggatcg gatctgatcc agaatcgact tgatcaccat
     1141 gaagattccg ccgaacacgg cagccacgat cacgcagatc agaatcatcg ccatgactgt
     1201 tccgatcact ccgctttcca ataattttaa aatgatcaga aacgccacca gcacaatggc
     1261 aagaaagccg gcaagcggtg ccatcacttt cccccacatc tcatttttct tcgtatcttc
     1321 cacgcaaatc cctccgcttt cgtatggtaa tgtataacac ttgccttcat tgtaccataa
     1381 ttaccgcaaa atacaaacag cttttacggc tgccccatgg caagctttgc cttcacgata
     1441 cgcgtcacgg attcattgat ccgctcctcg gtgatcgttc cgtcattcac cgcattcaga
     1501 attcctgtat aggcggtatt gtagtccgcc ggcatcaaaa tcatgtctgc cccggctgta
     1561 actgccagaa ccgccgcctt gtccgaattg tagaatccgg tgatcgcttc catattcatg
     1621 gcgtccgtaa tgacaattcc gttatatccc atctgtccgc gaagcacatc ggtaatcagc
     1681 accttcgaca gcgtagccgg cgcattgtct ccagtcacat tcggagccga gatatgtccg
     1741 accatcacaa aattcacgcc gccgtcaatc gcactctgga acgggacgag ttcttccgcc
     1801 atcagttcct ccagggtctt gtccgtggac accgccccgt catgagaatc tccagaagtg
     1861 ccgccgtgtc ccgggaaatg cttgaccgta ccgtacactc ccgccgcgga aagtccctgc
     1921 agttcagacg tcaccattcc cgcgaccgtc tgcgcgtccg acccgaacga gcgctgtccg
     1981 agcacctgat tgtccgggtt ggtaaggaca tccgccaccg gcgcgaaatc cacattgaat
     2041 ccgagtgccg caagatagct gccgatcgtg cttcccgcat tgtatgcatt ctgcacatcc
     2101 ccgccgccga tcactgccgt cactttcttc ttataa
//
LOCUS       DBMDQJ010000438         2135 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17849_length_2135_cov_1.753687, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000438 DBMDQJ010000000
VERSION     DBMDQJ010000438.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17849_length_2135_cov_1.753687"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1221
                     /gene="cls"
                     /locus_tag="ACZO9R_08875"
     CDS             <1..1221
                     /gene="cls"
                     /locus_tag="ACZO9R_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR0913211.1"
                     /translation="PVLFEQIPQDEAVLGRLEEQDFAVANQCRYIKRFGHYPVYRNTD
                     VTFYPDAAEGLAAQLHDLASAEHFIFMEYHAIEEAEAFGRIKDVLAERAAHGVEVRLF
                     YDDVGSIGFIDPGFIKRMEAIGVECRVFNPVLPILNIFMNNRDHRKITVVDGKIGYTG
                     GYNLADEYFNITHPYGVWKDTGIRLAGDAVASLTVTFLQMWNATAEADHEFQTYFPKL
                     DYRAADTGFVQPYADGPLTDGYFAEDVYLNLIKSAKHFFYAATPYLIISDDMTRELCL
                     AAERGVDVRIVTPGIPDKKLVYQTTRSYYAPLVRRGVRIYEYTPGFIHQKQTLCDGET
                     ATVGTINFDYRSLYHHFENGVLLHGCGAIGAIGEDFAQIFEVSREVTEQYKGKRSMGL
                     RIIQCILRLFAPLL"
     gene            1267..2037
                     /locus_tag="ACZO9R_08880"
                     /pseudo
     CDS             1267..2037
                     /locus_tag="ACZO9R_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080430.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
BASE COUNT          506 a          468 c          670 g          491 t
ORIGIN      
        1 ccggtgctgt ttgagcagat tccccaggat gaggcagtgc ttgggcggct ggaggagcag
       61 gattttgcgg tggcgaacca gtgccgctat atcaagcggt ttgggcatta tccggtgtac
      121 cggaataccg atgtgacatt ttacccggat gcggcggagg gacttgcggc gcagctgcat
      181 gatctcgcgt cggcagaaca ttttatcttt atggaatatc acgcgatcga ggaagcggag
      241 gcgttcggca ggattaagga cgtgctcgcc gagcgcgcgg cacacggggt ggaggtacgc
      301 ctgttttacg atgatgtcgg cagtatcggc tttatcgatc cgggctttat caagcgcatg
      361 gaagccatcg gcgtagagtg cagggtgttc aatccggtgc ttccgattct aaacattttc
      421 atgaacaacc gcgatcacag aaagattacg gtggtggacg gaaagatcgg ctacacgggc
      481 ggctacaatc ttgcggatga atattttaat atcacgcacc cgtacggagt ctggaaggat
      541 accggaatcc gtcttgcggg agatgcggtg gcaagcctga ctgtgacatt tcttcagatg
      601 tggaatgcca cggcggaggc ggaccatgag tttcagacgt attttccgaa gctggattac
      661 cgcgcggcag acaccggttt tgtacagccg tatgcggacg gccccctgac ggacggctat
      721 tttgcggagg atgtttattt gaatctcatc aagagtgcga aacacttttt ctatgcggcg
      781 acgccgtatc tgattatcag cgatgatatg acgagggaac tgtgtctggc tgccgagcgc
      841 ggcgttgatg tgcggattgt gacaccggga atcccggata agaagttagt gtatcagacg
      901 acacgctcct actatgcgcc tctggtgcgg cgcggcgtgc gtatctatga gtacacgccg
      961 ggatttatcc atcagaagca gacactttgt gatggggaga cggcgacggt cggaacgatc
     1021 aattttgact atagaagcct gtaccatcat tttgagaacg gcgtgctgct gcatggctgc
     1081 ggggcgatcg gtgccattgg agaggacttt gcgcagatat ttgaggtgtc gcgcgaggtg
     1141 acggaacagt ataagggaaa gcggagtatg gggcttcgca tcatacagtg tattctgcgg
     1201 ttgtttgcac cgctgttata ggagaagaaa aatgcgacgc attgcgtctg cggaacggag
     1261 gtttttatgc tgagagagtg gaaagagccg gtaagacccg gggaagagga attaaagatg
     1321 cgccttgcgg cagccaggga aaagcttgcc gcccagcagc ttctcatcaa ggagcagaaa
     1381 gtgccggtac tggtgctgct ggaaggctgg ggaacggcgg gaaaaggcta ctgcatcggt
     1441 cagatcatcg agaatattga cccgcgccat tttaaggtag agtccatgga gagaaagacc
     1501 ggggaggaag agcgcaagcc gtttttatac cgccatttta cccggattcc ggaggcggga
     1561 aagtttgtgt tcttagattc cggctggatg gatgaggtgg tggaagagcg ccttcacggg
     1621 gagctttccg aggaaaaata tgcaaagcgc atcgagagcg tgcggcgttt tgaacgtcag
     1681 ctgacggata acggctacct cgtgatgaag tttttcctgc atatctccaa gaaagagcag
     1741 accagacgga tggagcgcct cacggatgaa aaggacaccg tctggcgtgt gggaaaaaag
     1801 gatctgtggc agaacagtca ctacgagaag tgcatggatg cgttttcttc ctatttgaag
     1861 agcacgaata tgccgtccgc accgtggtat attgtggatg caaagtcgaa gaagtggacg
     1921 gaattgcagg tgcttgagac gctgacgcag ggaattgaca ttgcgcttga caaccgtgat
     1981 atggcggttc cgcttctgca gaatgtattc ccgttagagc gcatgccgct tctctcgctc
     2041 caggaaccat acaccatcca catacggtcg tcggcatcat aaaataccgt cgggtcgatc
     2101 gcatttggat attcctcaaa gttataactg ttgtt
//
LOCUS       DBMDQJ010000439         2130 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17891_length_2130_cov_1.507146, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000439 DBMDQJ010000000
VERSION     DBMDQJ010000439.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17891_length_2130_cov_1.507146"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            180..1400
                     /locus_tag="ACZO9R_08885"
     CDS             180..1400
                     /locus_tag="ACZO9R_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR0913212.1"
                     /translation="MSQYLLLKQGTIHNAVDEEAFVADILIEDGKIKKIAPVLEGKVI
                     NEAAVIDAMGIDVYPGFVDAHCHLGLDGYAVGFAGQDFNELGDPVTPQLSAIDAINPQ
                     DETFRMAREGGVTCVSTGPGSSNVIGGTFCIIKTYGNRVDDMIVREKSAMKIAFGENP
                     KNCYKEKGVYSRMSVAAKLREVLQKTIIYDKKLREAGCETSWNEINRGSGEGQVDISK
                     MPAYDAKLEAMLPVIRGEIPLKAHAHRADDICTAIRIAKEFHLGLRLDHVTDGALIAK
                     ELGAEHFPVAVGPSFGHATKYELKNKGFETPAILADAGCEVSIITDSPVIEERYLPLC
                     AGRAIYNGMREFDALRAITIQAARHIGAEDRVGSIEEGKDADFVLLKGTPFSVDARIL
                     YTIINGEIVYQSGE"
     gene            1528..2097
                     /locus_tag="ACZO9R_08890"
     CDS             1528..2097
                     /locus_tag="ACZO9R_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913213.1"
                     /translation="MKVESSSYEAYLEALEEWKQKMQETQATQQKEENSATDSYVPGI
                     SQMDMDIPMPSSTYNAQGMMSGERPPMPPMPPQETEETDETTESTQVTGTTETQGTAA
                     TGTEALLEELSETFRANPDSILMKLDELGLSLEDLGDEDNLASLATAMNEGAANMGLP
                     TIEDLDSAVSTLHEKLSANWSSYFEVEEA"
BASE COUNT          608 a          440 c          656 g          426 t
ORIGIN      
        1 aagatggaaa aatgtattta tacgatattc tgagaataaa aaaagaaacg agcgagccgc
       61 ttgagcaata gctgtacggt cgaaaaactc atttcttagt tgtattataa caggataact
      121 ataaaatgca agaaaaaatt aaggacagag cagggaaaga ataacacagg aggattgcga
      181 tgagtcagta tttattgtta aaacagggaa cgatccacaa tgccgtcgac gaggaggcat
      241 ttgtggcgga tattttgatt gaggacggaa aaataaaaaa gattgcgcct gtgctggagg
      301 gcaaagtcat caatgaagcg gctgtcattg atgcgatggg gatcgatgtc tatcccgggt
      361 ttgtggatgc acactgccat ctggggctcg acggttatgc ggtcggtttt gcggggcagg
      421 acttcaatga gcttggcgat ccggtgacac cgcagctctc ggcgatagat gcgatcaacc
      481 cgcaggatga gactttccgc atggcaaggg agggaggcgt tacctgcgtg tcgacaggac
      541 cgggaagctc caatgtgatc ggaggaacct tctgcatcat caaaacgtat ggaaaccgcg
      601 tggacgacat gattgtccgg gaaaaatcag ccatgaaaat tgcattcgga gagaatccga
      661 agaactgtta taaggaaaaa ggcgtctatt cgcgcatgtc tgtggcggca aaactgcggg
      721 aagtgctgca gaagacgatc atctacgata aaaagctgcg ggaggcaggc tgtgagacaa
      781 gctggaacga gatcaaccgc ggcagcgggg aggggcaggt ggacatcagc aaaatgccag
      841 cctacgatgc aaaactggaa gcaatgctgc cggtcatccg cggggagata ccgctgaaag
      901 cgcatgcaca ccgggcggac gatatctgca cggcaatccg cattgcaaag gagttccatc
      961 tggggttaag gctcgatcac gtgacggacg gcgcgctgat cgcaaaggaa ttgggtgcgg
     1021 agcattttcc ggtggcggtc ggtccgtcct tcggtcatgc gacgaagtac gagttgaaga
     1081 acaagggatt tgagacgcct gcaattctgg cggatgccgg ctgcgaggtg tcgatcatca
     1141 ccgattcgcc ggtcatcgag gagcgctatc tgccgctctg cgcagggcgg gcaatctaca
     1201 acggcatgcg tgagtttgat gcgctgcggg caattaccat tcaggcggca aggcatatcg
     1261 gcgccgaaga tcgtgtcgga agcattgaag aagggaagga tgcagatttt gttttgctaa
     1321 aaggcacccc gttttcggtt gacgcgcgga tattatatac catcataaac ggagagattg
     1381 tctatcagtc cggggagtaa tgtgacggtt atctgaatgt tatgtgaagt ctgttatttt
     1441 ttctgcggtg gaaccgatag ctattttgag gaaaatgcaa aggatggcac aatttcggga
     1501 aggaaccacc tcagaaagga attggttatg aaggtcgaga gttcaagtta cgaggcatat
     1561 ctggaagcac tggaagagtg gaagcagaag atgcaggaga cccaggccac acagcagaag
     1621 gaagagaaca gcgccacgga cagttatgtg ccgggcatca gccagatgga catggacatt
     1681 ccgatgccga gcagcactta caatgcacag gggatgatga gcggagaacg cccgccaatg
     1741 ccgccgatgc cgccgcagga gacagaggag acagacgaga cgacggagag cacacaggtc
     1801 acgggaacga cagagacgca gggaaccgca gcgacaggaa cagaggcatt gcttgaggag
     1861 ttgtcagaga cattccgcgc aaatccggac agcatcctga tgaagctgga tgaattggga
     1921 ttatcgctgg aggatctggg tgacgaggat aatcttgcat cacttgccac cgcaatgaac
     1981 gaaggagcgg caaatatggg cttgccaacc attgaagatc tggattctgc ggtaagcacc
     2041 ctgcatgaga agttgtccgc gaactggagc agctactttg aagtggagga agcatagatc
     2101 tcttttgggg ataagtaata ttataagaaa
//
LOCUS       DBMDQJ010000440         2129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17904_length_2129_cov_1.745069, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000440 DBMDQJ010000000
VERSION     DBMDQJ010000440.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17904_length_2129_cov_1.745069"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="ACZO9R_08895"
     CDS             complement(<1..476)
                     /locus_tag="ACZO9R_08895"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR0913214.1"
                     /translation="MHMTDYKKDFPLLAESDIAYLDNAATEQRPACVLDAEREFYERW
                     NANPLRGLYELGVEATEQYEHARERVARFVHAKSEREIIFTRNTTEGINLVAYSYALP
                     QLKPGDEILVTIMEHHSNLLPWQMAARRTGATLKYLECEPDGSFTEEKIRDAVTPHT"
     gene            complement(467..1555)
                     /locus_tag="ACZO9R_08900"
     CDS             complement(467..1555)
                     /locus_tag="ACZO9R_08900"
                     /inference="COORDINATES: protein motif:HMM:NF013614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufB/SufD family protein"
                     /protein_id="HIR0913215.1"
                     /translation="MTKQNQAITVNRLPAKTWNWLKMNETVIGMPRIDGSIAIKQSVA
                     EAGQAEDEQLRMAQQTDWDKITTGMGEAMDQPSETDVAWDTELLTIPEHADCGEPYVY
                     RFSYQSGQHAYNRLHIYAGAHSRAKVILLCESGETGGMMSAIQTKVYAGEGAKVELYV
                     VQTMNAEAVCLHDVGVSCEKDAAVKEVQLELGGAEVYTGTLFELNGEESTAELVSGYL
                     GTGRQKIDMNYVARHRGKKTKSNMQISGILKNEAKKLMRGTIDFVHGCVGAKGDEKED
                     VLLVGDNMVNQTIPLILCAEEDVEGNHGASIGKLDENMLFYAATRGIPPEEAQRLLTL
                     AKIEAINSQIPSEEVRGAVETYLKEQCI"
     gene            complement(1567..>2129)
                     /locus_tag="ACZO9R_08905"
     CDS             complement(1567..>2129)
                     /locus_tag="ACZO9R_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008399953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HIR0913216.1"
                     /translation="EGGRIEWVSGSFGSHVSYLYPMSILKGKGAHMEFTGITFAGKGQ
                     NLDTGAKVVHAAPDTSSYMNTKSISKDGGISTFRSSVSITKEAAGSKSAVSCQSLMLD
                     DISRSDTIPAMDIRTGDADVGHEAKIGRISDDAVFYLMSRGISEEEARSMIVSGFASN
                     VSKELPLEYAVEMNNLIDLEMKGSIG"
BASE COUNT          398 a          683 c          485 g          563 t
ORIGIN      
        1 gtgtgcggtg tgacggcatc acggattttc tcctccgtaa aactgccgtc cggttcacac
       61 tccagatatt taagcgtggc tccggttcgt ctggccgcca tctgccacgg aagcaggttg
      121 ctgtgatgct ccatgatcgt gacaagaatc tcatccccag gctttaactg gggcagcgca
      181 tagctgtacg ccaccaggtt gatgccctcg gtcgtatttc tggtaaaaat gatttctctc
      241 tcactcttcg catgtacaaa ccgtgcgacg cgctctctcg catgctcata ctgctctgtc
      301 gcctccacgc ccagctcata gagcccgcgc agcggattgg cattccagcg ctcatagaat
      361 tcccgctccg catcaagcac gcacgccggc cgctgttctg tcgccgcgtt gtcgaggtaa
      421 gcgatatcgc tttccgcaag cagcggaaaa tcctttttat aatctgtcat atgcattgct
      481 ccttcagata agtttcaaca gcgccgcgca cctcctctga cgggatctgg ctgtttatcg
      541 cttcgatctt cgccagtgtc agcagtctct gtgcctcctc cgggggaatg cctctcgtgg
      601 cggcataaaa gagcatgttc tcgtcgagct tgccgatgga agcgccgtga ttgccctcca
      661 catcctcctc cgcgcacagg attaacggga tggtctggtt gaccatattg tcaccgacaa
      721 gaagcacatc ctccttctcg tctcccttag ctcccacaca gccgtgcaca aaatcgatcg
      781 ttccgcgcat cagcttcttc gcctcgttct ttaagatacc cgatatctgc atattgctct
      841 tcgtcttttt cccgcggtgt ctggcgacat aattcatatc aatcttctgc ctgccggtgc
      901 ccagatatcc gcttacgagt tctgccgtgc tctcttctcc gttaagttca aacagtgttc
      961 ccgtatagac ctcggcgccg cccagttcca gctgcacttc cttcacagcg gcatcttttt
     1021 cacacgagac acccacatca tgcaggcaga cggcttccgc attcatcgtc tgcaccacat
     1081 acagttctac tttcgcgccc tccccggcat ataccttcgt ctggatggcg gacatcatcc
     1141 cgcccgtctc cccgctctcg cagagcagga ttacttttgc ccggctgtgc gctccggcat
     1201 agatatgcag gcggttatag gcgtgctgcc cgctttggta ggaaaaacgg tacacatacg
     1261 gttcgccgca gtctgcatgt tccggaatcg tcaaaagctc ggtatcccat gccacatctg
     1321 tctccgacgg ctgatccatc gcctcgccca tcccggttgt gattttatcc caatccgtct
     1381 gctgtgccat ccgcagctgc tcatcctctg cctgccctgc ttctgccaca ctctgtttta
     1441 tcgctatact gccgtcgatc cgcggcatcc caatcacggt ttcattcatt ttcagccagt
     1501 tccaggtctt tgcaggaagc cggttgactg tgatcgcctg attctgtttt gtcataagct
     1561 cctcctttac ccgatgctgc ccttcatctc aagatcaatc agattgttca tctccaccgc
     1621 atactctaac ggcagttcct ttgacacgtt gcttgcaaaa ccactgacaa tcatcgaccg
     1681 cgcttcctcc tcgctgatgc cgcgggacat cagatagaat acagcgtcgt cgctgatgcg
     1741 gccgatttta gcctcatgtc ccacatccgc gtctcctgtg cggatgtcca tcgccggaat
     1801 cgtatccgat cttgagatgt catcgagcat cagcgactga caggaaactg ccgatttgct
     1861 gccggcggct tcctttgtaa tgctcacgga actccggaac gtgctgatac cgccgtcctt
     1921 ggagatcgac ttggtgttca tgtacgagga ggtatccggt gcggcatgca ccacctttgc
     1981 cccggtgtcc agattctgac cttttccggc aaacgtgatg cctgtgaatt ccatgtgcgc
     2041 cccttttccc tttaagatac tcatcggata gagatacgat acatgggagc cgaaggagcc
     2101 ggacacccac tcgatccgtc ccccctcct
//
LOCUS       DBMDQJ010000441         2129 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17905_length_2129_cov_1.444280, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000441 DBMDQJ010000000
VERSION     DBMDQJ010000441.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17905_length_2129_cov_1.444280"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(168..>2129)
                     /locus_tag="ACZO9R_08910"
     CDS             complement(168..>2129)
                     /locus_tag="ACZO9R_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR0913217.1"
                     /translation="GKIAGLLSNAKEKKTPLQIALDDFGKKLSYLILAVCALVFGLSM
                     YRDMPLLDAMMFAVALAVAAIPEALGSIVTIVQAMGTQKMAREHAIIKDLKAVESLGC
                     VSVICSDKTGTLTQNKMTVQKIYLDHTAIMPEALQINDQLHRYLMYDAILNNDARMDD
                     GKVIGDPTESALLEMVRKCGVDDDFLRGAMERQQELPFDSDRKLMSTVYALHGVPTLL
                     TKGAPDVLLKRAVYLREKQGIRRMTEEDRTEILEQNQRFSGEGLRVLAFAYRELKEGE
                     KLTISGECDYIFLGLVAMIDPPRAESAAAVADAKRAGIRPVMITGDHKVTATAIAKQI
                     GIFGEGDIALVGPELDAMPDKELDHLIGHIAVFARVSPENKIRIVEAWQRRGSVVAMT
                     GDGVNDAPALKAADIGVAMGITGTEVSKDAAAMILTDDNFATIIKAVANGRNVYKNIK
                     NAILFLLSGNMAGILAVLYTSLAALPVPFQPVHLLFINLLTDSLPALAIGMEPAESDV
                     LSEKPRNPKGGILSRRFMALLLGHGALIAVCTMISFEVGLKESAALASTMAFATLTLA
                     RLFHGFNCRSRHSIVKIGFRRNWYSLGAFALGVLLLALVLFVPALETLFVVEKMNGMQ
                     LGCVGVMAVLPTFVIQSFKVVGEVSGREK"
BASE COUNT          487 a          691 c          459 g          492 t
ORIGIN      
        1 catccgctcc gcacgccgca cttccagcaa gcaacgccac tgccagtagt gccgccagtt
       61 ctctctttcc tcttcgtctc atccaaacgc ccctctttct gtcagatttt ctgtatcctt
      121 tttccttatc atacaggact ggtaacgttt tcacaagctg tttcttccta cttttctctg
      181 ccagacactt cacccaccac cttgaagctc tggatcacaa acgtcggcag caccgccatc
      241 acaccgacac agcccagctg catgccattc atcttctcca ccacaaaaag agtttcgagt
      301 gccggcacaa acagaacaag tgccaaaagc agcacaccga gcgcaaaggc tcccagactg
      361 taccagtttc tccggaatcc gatctttaca atggagtgac ggcttctgca gttaaacccg
      421 tggaacaagc gtgcaagtgt cagtgtcgca aacgccatgg tgctcgccag cgccgcactc
      481 tctttcaatc ccacctcaaa ggaaatcatg gtacagacgg caatcagcgc cccatgcccc
      541 agaagcagcg ccatgaagcg ccgggagaga attccgccct tagggttacg cggtttctcg
      601 ctgaggacat cgctctccgc cggttccatg ccgatggcaa gtgccgggag agaatcagtc
      661 agcagattga taaacaaaag atgcaccggc tggaacggga ccggaagtgc tgcaagcgag
      721 gtgtaaagca ccgccaatat tcctgccata tttccggaaa gtaaaaacag gattgcattt
      781 ttaatattct tgtagacatt tcttccgttc gcgaccgctt tgataatcgt ggcaaaattg
      841 tcatcggtca ggatcatggc tgccgcgtct ttggacacct cggttccggt gattcccatc
      901 gccacgccaa tgtccgccgc cttcagtgcc ggtgcatcat tgacgccgtc tcccgtcatg
      961 gcgaccacac tccccctgcg ctgccacgct tccacaatac ggatcttatt ttccggagag
     1021 acacgggcaa acacggcaat gtgcccgatc agatgatcca gctccttatc tggcatggca
     1081 tccagttcgg ggccgaccag tgcgatatcc ccctccccga aaatgccaat ctgctttgca
     1141 atcgccgtcg ccgtcacttt atgatccccc gtaatcatga ctggtcggat tcccgcccgc
     1201 ttcgcatctg ctacagccgc cgcagactct gcgcgcggcg ggtcgatcat cgccacaagc
     1261 ccaagaaaaa tgtaatcaca ctccccggag attgtcagct tttccccctc cttcagttcc
     1321 cggtaagcaa aagccaacac acgaagcccc tcgccggaaa atctttgatt ctgctctaaa
     1381 atctccgtac ggtcttcctc cgtcatccgc cggataccct gcttctccct aagatagacc
     1441 gcgcgcttca aaaggacgtc cggcgcccct ttggtcagca gtgtcggtac accgtgcagc
     1501 gcatagaccg tgctcatcag cttgcggtcc gaatcaaacg gcagttcctg ctgtcgttcc
     1561 attgctcccc gtaagaaatc atcatccacg ccacatttac gcaccatctc gagcagtgct
     1621 gactccgtcg ggtcgccaat cactttcccg tcatccatgc gcgcatcgtt atttaaaatc
     1681 gcatcataca tcagataacg gtgaagctga tcgttgatct gcagcgcctc cggcatgatc
     1741 gccgtatgat ccagataaat cttctgcacg gtcattttgt tctgtgtaag cgtgcccgtc
     1801 ttatccgaac agatcaccga cacacagccc agactctcca ccgccttcag atctttgatg
     1861 atcgcatgct ccctcgccat tttctgggtt cccatcgcct gcacgattgt cacgatcgag
     1921 ccgagggctt ccggaatcgc cgcaactgca agcgcgaccg caaacatcat cgcgtccaga
     1981 agcggcatat cccggtacat tgacagaccg aacaccagcg cacagacagc cagaatcaga
     2041 tatgacagct tttttccaaa atcatccaat gcaatctgca gcggcgtctt tttttccttc
     2101 gcattgctta aaagtcctgc aatcttacc
//
LOCUS       DBMDQJ010000442         2124 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_17949_length_2124_cov_1.767672, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000442 DBMDQJ010000000
VERSION     DBMDQJ010000442.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17949_length_2124_cov_1.767672"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1955
                     /locus_tag="ACZO9R_08915"
     CDS             <1..1955
                     /locus_tag="ACZO9R_08915"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004557 - alpha-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR0913218.1"
                     /translation="FIGLFASRAKAWRTAEHYLFAVPGFWDYPCTYEITEKTAETENG
                     DLPGAYDEKGEAQRLSIRLRDHSYDLVLELHYDVYAECDAIVRSAVLFNESGEMIRLN
                     RLMSIQIDFDPAEYVFTTFTGAWAREMHRSDTRMEAGKHVNASYTGTSSSRANPFVMI
                     AKTDTTEDTGECYGCNLIYSGNHYEAAEVSGYGKMRLTAGINPQSFSWLLAPGENFAA
                     PEAVMAYSCEGYNGMSQCMHAFVREHIVRGAFKHKVRPVLLNSWEVAYFDINERKLLA
                     LAKKAKEAGVELFVMDDGWFGERNDDAHSLGDWEVNEKKLPGGLAGLGRKIKALGLDF
                     GIWVEPEMVNVNSHLYQAHPDWTIEIPGKPHAEGRNQRILDLTRTEVQDYIIETMTKV
                     FSSAEISYVKWDMNRTFTDYYSRALPPERQGEVAHRYVLGLYRCMRELTARFPDILFE
                     GCSAGGNRFDLGILSYFPQIWASDDTDALCRAEIQTGYSYGYPMSVVSAHVSACPNHQ
                     TLRVTPLETRFAVAAFGICGYECNFCDLSSEDFAAVKAQIALYKQWREVLQKGRFYRG
                     RTFGEGAHESALSQSVGNQMEWTCVSEDQTRAVGMLMQKLVVPNTQYHSYQAKGLKPD
                     ARYHFYNRSLKYNIKDLNDIIGGAHK"
     gene            1952..>2124
                     /locus_tag="ACZO9R_08920"
     CDS             1952..>2124
                     /locus_tag="ACZO9R_08920"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine 5'-phosphate decarboxylase / HUMPS
                     family protein"
                     /protein_id="HIR0913219.1"
                     /translation="MSKDVIIACDFNSKADLMTFLDKFENEDRKPFLKVGMELFYAEG
                     PAIVKEIKARGHK"
BASE COUNT          512 a          493 c          651 g          468 t
ORIGIN      
        1 tcttcatcgg cctgtttgcc tcccgtgcga aggcttggcg gacagcggaa cactatttat
       61 ttgctgtccc cggattttgg gactatccct gtacttatga gatcacggaa aagacggcgg
      121 agacagaaaa cggagatctc ccgggagctt acgatgagaa aggggaggcg cagcgtctta
      181 gcataaggct gcgcgaccac tcctatgatc ttgttttaga acttcattat gacgtctacg
      241 cagagtgcga tgcgattgtg cgaagcgccg ttctctttaa tgagagcggg gagatgattc
      301 gtctgaatcg cctgatgagt atccagatag actttgatcc ggcagagtat gtgttcacaa
      361 cctttactgg tgcgtgggcg agggagatgc accgttccga tacgcgcatg gaggcgggaa
      421 aacatgtcaa tgcgtcctac acgggaacct cctccagccg tgccaacccg ttcgtcatga
      481 ttgcaaagac ggatacgacg gaggataccg gggaatgtta cggatgcaac ctcatctaca
      541 gcggcaacca ctatgaggcg gcggaagtga gcggctacgg caagatgcgc ctgactgccg
      601 gcatcaatcc gcagtcattt tcctggctgc tggctccggg ggagaacttt gcggcgccgg
      661 aggcggtcat ggcgtattcc tgtgagggct acaatggcat gagccagtgc atgcatgcat
      721 ttgtgcggga gcacatcgtg cgtggagcgt ttaagcacaa ggtgcgtccg gtgctgttaa
      781 acagttggga ggtagcctat tttgacatca atgagagaaa gctgcttgct ctggcgaaga
      841 aggcaaagga agccggcgtg gagctttttg tgatggacga cggctggttc ggcgagcgca
      901 atgatgacgc acattccctg ggagactggg aagtcaacga gaagaagctt ccgggaggtc
      961 tcgcgggact cggaagaaag atcaaggcgc tcgggctgga tttcggtatc tgggtggagc
     1021 cggagatggt gaatgtgaac agtcatctct accaggcaca tccggactgg acgatcgaga
     1081 ttccgggaaa accgcacgcg gagggacgca atcagcgcat tctggatctt acgagaacag
     1141 aagtgcagga ctatattatt gagacgatga caaaggtatt ttcctcggcg gagatctcct
     1201 atgtcaaatg ggatatgaac cggacgttta cggattacta ttccagagcg cttccgccgg
     1261 agcggcaggg agaggtggca caccgctatg tgctgggact gtaccgctgc atgagagaac
     1321 ttaccgcgcg ttttccagat attctctttg agggatgctc ggcgggcggc aaccggttcg
     1381 atctcggaat tttgtcctat tttccacaga tctgggcgtc cgatgatacg gatgcgctct
     1441 gccgggcaga gatccagacc ggctattcct acggctatcc gatgtccgtt gtgagcgccc
     1501 atgtgtcggc gtgcccgaac caccagacgc tccgcgtgac gccgcttgag acacggttcg
     1561 ctgtggcggc gttcggtatc tgcggctatg agtgcaattt ctgcgatctg tccagcgagg
     1621 attttgcggc ggtaaaggcg cagatcgcac tgtacaaaca gtggcgggag gtgttacaga
     1681 agggacgttt ttaccgcgga cgcacattcg gagagggggc gcatgaaagt gcactctccc
     1741 agagcgtggg aaaccagatg gagtggacct gcgtgtcgga agaccagacc cgcgcggtgg
     1801 ggatgctgat gcagaagctg gtcgtaccga atacccagta tcacagctat caggcgaagg
     1861 gattaaaacc cgatgcgcgg tatcatttct ataaccgcag cttaaaatac aatatcaagg
     1921 atttaaacga catcattgga ggtgcccata aatgagtaag gatgtaatca ttgcctgcga
     1981 ttttaacagc aaggcagatt tgatgacatt tttagataaa tttgaaaacg aagacagaaa
     2041 gcctttctta aaggtcggca tggagctgtt ctatgcggaa ggccctgcca tcgtaaagga
     2101 aatcaaggca agaggacata agat
//
LOCUS       DBMDQJ010000443         2117 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18024_length_2117_cov_1.316964, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000443 DBMDQJ010000000
VERSION     DBMDQJ010000443.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18024_length_2117_cov_1.316964"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="ACZO9R_08925"
     CDS             <1..1285
                     /locus_tag="ACZO9R_08925"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HIR0913220.1"
                     /translation="WNSYDYYDTTVTEREVRANADYMAAHLKPYGWEYIVVDIQWYAK
                     NTGSQREKYQYIPFGEVSMDEYGRLLPCTDRFPSAKDGVGFRALADYVHGLGLKFGIH
                     IMRGIPREAAHRHLPILGSDALASDIADPSSICKWNPDMYGIRMGEPGAQEYYDSIVA
                     LYAQWGVDFIKCDDICNTNLYVEHPYSAAHEIEMLQHAIEKCGRDIVLSLSPGPALIE
                     KAWHYETYANMWRITDDFWDTWELLYDMFRRCELWQNHVGCGSFPDCDMLPVGWLGKG
                     FGQERQTNFTRDEQKTMMTLWCMFGSPLMIGGELTKLDDWTQFLLTRRELLQMLDADY
                     VGRQVARDQKHAVWSCVNEKKDERYLALFNFMEQPARCEVALPETEAFADMCGQTGTI
                     RARELWDGTELIVQNDSLSSEIPAHGVHVFRIDKK"
     gene            1435..>2117
                     /locus_tag="ACZO9R_08930"
     CDS             1435..>2117
                     /locus_tag="ACZO9R_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR0913221.1"
                     /translation="MGNAKERIESGKAVLGIEFGSTRIKAVLVDETHQPIASGAHDWE
                     NQLVDGIWTYSLDMIWDGLRDCYRKMTEDVQNKYGVAVTKLGAIGFSGMMHGYMPFDE
                     KGELLVPFRTWRNTITGEAAAALTKEFSFNIPQRWSIAHLYQAILNGEEHVDKIAYFT
                     TLAGYIHWKLTGEKVLGVGEASGMFPIDAKTRDYNRKMLAQFDALPAVGKYTWKLEEI
                     LPKVLVAGE"
BASE COUNT          535 a          442 c          681 g          459 t
ORIGIN      
        1 gtggaacagc tatgactatt atgacaccac agtgacggag cgggaggtca gggcaaacgc
       61 ggattatatg gcggcgcact taaaaccgta cggctgggaa tatattgtgg tggacatcca
      121 gtggtatgca aagaacaccg gaagtcagag ggagaagtac cagtatattc cgtttgggga
      181 ggtctcgatg gatgagtacg ggcggctgct cccctgtacg gaccgcttcc cgtcggcgaa
      241 ggacggtgtg ggctttcggg cgctggcgga ttatgtgcat gggctggggt taaagtttgg
      301 aatccatatc atgcggggaa ttccgcggga agcggcacac cggcatctgc cgattttggg
      361 aagcgatgca ctggcgagcg atatagcgga tccgtcgtcg atctgtaagt ggaatccgga
      421 catgtacggc attcgtatgg gagagccggg cgcgcaggaa tattacgatt ccatcgtggc
      481 actgtacgca cagtggggcg tggattttat caaatgcgat gatatctgca ataccaatct
      541 gtacgtggaa catccgtatt cggcggcgca cgagattgag atgctgcagc atgcgattga
      601 aaagtgcggc agggacatcg tgctctcctt gtcgccggga ccggcgctga tcgagaaagc
      661 atggcattat gaaacctatg ccaacatgtg gcgcatcacg gatgatttct gggatacgtg
      721 ggagctgctt tacgatatgt tccggcgctg cgaactgtgg cagaaccacg tgggctgcgg
      781 atcgttcccg gactgtgaca tgctgccggt cggctggctc ggaaaaggat tcgggcagga
      841 gcggcagacg aattttacaa gggatgagca gaagacgatg atgacgctct ggtgtatgtt
      901 cggctcgcct ttgatgatcg gcggtgaact gacgaagctg gatgactgga cgcagttcct
      961 tttaacgaga cgggagctgc tgcagatgct cgacgcagac tatgtgggca gacaggtggc
     1021 gcgcgatcaa aagcatgcgg tctggagctg tgtcaatgag aaaaaggacg aacggtatct
     1081 tgccctgttt aactttatgg agcagccggc gcggtgtgag gttgcgctgc cggagacgga
     1141 agcgtttgca gacatgtgcg gacagacggg aaccatccgg gcgagggaac tctgggatgg
     1201 aacagagctt attgtgcaga atgacagcct ttcttctgaa attccggcgc acggtgtgca
     1261 tgttttccgg atagataaaa aatagagaac cacacaatgt aaaaataaat aaaaaacaac
     1321 ttgttattac gagcatacaa gttttatgct taagataaca tcaataagat gagtgcaggg
     1381 aaatcatggg tgcatgagag atgcggcatg gtttcggaaa ggggtttgtt acacatggga
     1441 aatgcaaagg agaggattga gagtgggaag gcggtgctcg gtatcgagtt cggctcgacc
     1501 agaatcaagg cagtgctggt ggacgagaca catcagccga tcgcatccgg tgcgcacgac
     1561 tgggagaatc agctggtcga cggaatctgg acgtacagcc tggatatgat ctgggacggt
     1621 cttagggact gctaccgcaa gatgacagag gatgtacaga acaaatacgg cgttgccgtc
     1681 acaaagcttg gtgccatcgg attttccgga atgatgcacg gctatatgcc gttcgatgag
     1741 aagggggagc ttctggttcc gttccgcacc tggagaaata ccatcacggg cgaggctgcc
     1801 gcagcactga cgaaggagtt ctcgtttaat attccgcagc gctggagcat cgcacatctg
     1861 tatcaggcaa tcttaaacgg ggaagagcat gtggataaaa tcgcatattt taccacgctt
     1921 gcgggatata ttcactggaa actcactggg gagaaggtac tgggagtcgg agaggcatcc
     1981 ggtatgttcc cgatcgatgc gaagacaaga gattacaacc ggaagatgct ggcacagttt
     2041 gatgcgcttc ctgcggtagg gaaatataca tggaagttag aggagattct tccgaaggtg
     2101 ctcgtggcag gggagaa
//
LOCUS       DBMDQJ010000444         2102 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18177_length_2102_cov_1.178911, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000444 DBMDQJ010000000
VERSION     DBMDQJ010000444.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18177_length_2102_cov_1.178911"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..563)
                     /locus_tag="ACZO9R_08935"
     CDS             complement(156..563)
                     /locus_tag="ACZO9R_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913222.1"
                     /translation="MADTMKMEYKIFLEAEDVSQSRILSCASYMKRVLESCNNPYISR
                     AELDDESDLDDFVLRLFVEEEIEEKECTNPAMAESFIEDMAELVTGIAEAHSFLDLEG
                     SFSVTWKGTTSAYAFVSPGGDDGCDFQELGVTE"
     gene            complement(601..1533)
                     /gene="metA"
                     /locus_tag="ACZO9R_08940"
     CDS             complement(601..1533)
                     /gene="metA"
                     /locus_tag="ACZO9R_08940"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004414 - homoserine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase MetA"
                     /protein_id="HIR0913223.1"
                     /translation="MPIRIQSDLPAKEILEKENIFVMDENRAMHQDIRPIKIGILNLM
                     PLKEETELQLLRSLSNTPLQVDVTFVAVSSHESKNTSMSHLNKFYESFADIKRRYFDG
                     LIITGAPVEQMEYEDVDYWEEICQIFEWTKSHVTSTLHLCWGAQAGMYYHYGLKKYLL
                     PQKKFGVYEHRVMNRKVPLVRGFDDCFMAPHSRHTEVRTEDIRKVKELTILAESEEAG
                     VFLAIADEGRRIFVMGHPEYDRYTLDKEYKRDKEKGLPIEPPVNYYPDDDDTKKPNLQ
                     WRSHGNILYSNWLNYYVYQQTPYEFIDTAEIIGK"
     gene            complement(1541..>2102)
                     /locus_tag="ACZO9R_08945"
     CDS             complement(1541..>2102)
                     /locus_tag="ACZO9R_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR0913224.1"
                     /translation="GIIGKEKNTDKLLEAIEKAKQNDAYKLLTGLGIPNVGKAAAKAI
                     MKHFKTMERLSEASEEELTAVGDIGKVSADCIRSYFSDEKNQVVLQRLAQAGVNMTAE
                     ESETVDSVISGKTLVVTGTLPTLGRKEAQTLIERYGGKVSGSVSKKTDYVLAGESAGS
                     KLTKAQELGIRVISEDELYDMLQIER"
BASE COUNT          461 a          646 c          412 g          583 t
ORIGIN      
        1 gcaaccactt catctgcaat cttcacttct cccaaattac cgtcctttat tttaaatgtt
       61 ttgcgatcct cacccatgtc aaatgcctcc taagtgatat tatgcgttta ttataacaga
      121 ctattcccgt tttggaaacc aaaaaaagta ttaaattatt cggtcacacc gagttcctga
      181 aaatcgcatc catcatcacc gcctggggaa acaaaggcat atgcgctcgt cgttcccttc
      241 catgtgacag aaaagcttcc ctccagatcc aggaacgaat gtgcttcagc aatccccgtc
      301 acaagctccg ccatatcctc gataaagctc tccgccatcg ccgggttggt gcactccttc
      361 tcctcgatct cctcctccac gaaaaggcgc agcacaaaat catccagatc gctctcgtca
      421 tcaagttctg ccctgctgat ataaggatta ttacagctct ctaacacacg cttcatgtag
      481 gaagcacagg aaaggatccg cgactgcgac acgtcctccg cctccaggaa aatcttgtat
      541 tccatcttca ttgtatctgc catcttatgt cctcttttcc gtgccttggc aaacgccgca
      601 tcactttcct ataatctccg ccgtatcaat gaactcataa ggagtctgct gatagacgta
      661 ataatttaac cagttgctgt aaaggatatt gccgtgcgag cgccactgca ggttcggctt
      721 tttcgtatcg tcatcgtccg gataatagtt gaccggcggc tcaatgggaa gccccttctc
      781 cttgtcccgc ttgtattcct tatccagcgt gtagcggtca tactccggat gccccatcac
      841 aaagattctt ctgccctcgt ccgcaatcgc gaggaatacg cccgcctcct cagactccgc
      901 aagaatggtc agctccttga ctttgcggat gtcctccgtg cgcacctcgg tatgtctgga
      961 gtgcggtgcc ataaaacaat cgtcaaatcc gcgcaccagc ggcactttcc ggttcatcac
     1021 acggtgttca tagacgccga actttttctg cggcagaaga tattttttca gtccgtagtg
     1081 atagtacatt cccgcctgtg ccccccagca cagatgcagg gtggacgtca catggctctt
     1141 cgtccactca aagatctggc agatctcctc ccagtaatcc acgtcctcgt attccatctg
     1201 ctccaccggt gccccggtga tgatcagccc gtcaaaatag cgtctcttaa tatccgcaaa
     1261 actctcgtag aatttattca gatgactcat cgacgtattc ttggactcat ggctggacac
     1321 cgcaacaaat gtcacatcga cttgaagcgg cgtgttcgac agggaacgca gaagctgcag
     1381 ttccgtctct tctttcagcg gcatcagatt taaaattccg atcttgatcg gtctgatgtc
     1441 ctgatgcatc gcacgattct catccataac aaatatattt tccttctcca gtatttcctt
     1501 tgcaggtaaa tcactctgta ttctgattgg cataaaaatg ttacctctct atctgcagca
     1561 tgtcgtacag ttcgtcctca ctgatcactc tgatgccgag ttcctgcgcc ttcgtaagct
     1621 tgcttcccgc gctctccccc gcgaggacat aatccgtctt ctttgacaca gatccggata
     1681 cctttcctcc ataccgctcg atcaacgtct gcgcttcctt tctaccaagc gtcggcaggg
     1741 ttcccgtcac cacaagggtc tttccgctaa taacagagtc cacggtctcg ctctcctccg
     1801 ccgtcatgtt gacgccggcc tgtgcgagtc tctgcagcac cacctggttc ttctcatccg
     1861 aaaaataact tcggatgcag tccgcactga cttttccgat gtccccgact gccgtcaatt
     1921 cctcctcgga tgcctccgaa agccgctcca tggtcttaaa atgcttcatg atcgccttcg
     1981 ctgccgcctt tccgacattc ggtatcccaa gaccggtcaa cagcttgtac gcatcattct
     2041 gcttggcttt ttctatcgcc tccagaagct tgtccgtgtt cttctccttg ccgatgattc
     2101 cc
//
LOCUS       DBMDQJ010000445         2096 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18236_length_2096_cov_1.648120, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000445 DBMDQJ010000000
VERSION     DBMDQJ010000445.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18236_length_2096_cov_1.648120"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="ACZO9R_08950"
     CDS             <1..221
                     /locus_tag="ACZO9R_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HIR0913225.1"
                     /translation="GVNYEELGTKTADMAVDILVNGADPAATPIETLERGIVTVNTET
                     AEALGLDYSMFKDMCSEVVETVTAEEFE"
     gene            223..1191
                     /locus_tag="ACZO9R_08955"
     CDS             223..1191
                     /locus_tag="ACZO9R_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR0913226.1"
                     /translation="MGPIITLSIVQSALELGFIYALVALALFISFSILNIADLSTDGC
                     FTLGCAVCASVTLAGHPVLGLLAAMAAGVCSGFVTAFLQTRLGIQSILAGIIVNTGLY
                     TINIAVMGFASNLNLFACDSVFTWAKDAIGGTWYKLIVAAVIVALVGVLISLFLNTRL
                     GLSIRATGDNPDMVRASSINTGMMITIGLCVANALTGLSGGLLAQYQKSCDINLGTGM
                     VTIALASLIIGETIIGKGSMLKRVIGVILGSCLYRFIVAVALRLNVPAECLKLVSSII
                     VAVAIAFPYLKKQAVFYRQKRAAKAENEQYIAELLREERKGKGGNA"
     gene            1214..2032
                     /locus_tag="ACZO9R_08960"
     CDS             1214..2032
                     /locus_tag="ACZO9R_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0913227.1"
                     /translation="MLIMKNVSKTFNPGTVNEKCALNGVNLTLGAGDFVTIVGSNGAG
                     KSTLFNAITGAFFPDRGLIMLGGEDITYQQEHVRSKVIGHLFQDPLKGTAPHMTIEEN
                     MALAYLRTTTSRNAYFSRINAADKKKFREQLALLDMGLEDRMKQPVGLLSGGQRQALT
                     LLMATMVTPKILLLDEHTAALDPATAEKVLHLTRQIVAERQITCLMVTHNMHQALTLG
                     NRTLMMNAGNIVLDVAGEERAAMSVDDLLEQFALRAGKALDNDRILFSKVETQE"
BASE COUNT          523 a          469 c          624 g          480 t
ORIGIN      
        1 acggagtcaa ttatgaggaa cttggtacaa agacagccga tatggcagtc gatattctgg
       61 taaacggagc cgatccggcg gcaactccga tcgagacctt agagcgcgga attgtgaccg
      121 tgaatacaga gacggcggaa gcacttggac tggactactc catgtttaaa gatatgtgtt
      181 cggaagttgt tgagacagta acggcagagg agtttgaata atatgggacc aatcatcacc
      241 ttatcgatcg tgcagagtgc actggaactg ggatttatct atgcactggt tgcacttgca
      301 ctgttcattt catttagtat tttaaatata gcggatctgt caacggacgg atgctttacg
      361 ctgggatgtg cagtgtgcgc ttcggttaca ctggcggggc atccggtact cgggcttttg
      421 gcggcgatgg ctgccggtgt gtgttccggt tttgtgacgg catttttgca gacaagactc
      481 ggcatacagt cgattctggc aggaattatt gtcaacaccg gactttacac aatcaatatt
      541 gcggtgatgg gattcgcctc caacctgaat ctgtttgcct gtgattccgt atttacatgg
      601 gcaaaagacg cgatcggcgg aacctggtat aagctgatcg tggcggcagt gattgtcgca
      661 ttggtcggcg tattgatctc tctgttttta aatacgagac tcggactttc catccgggca
      721 accggggata atccggatat ggtgcgggca tccagcatca atacgggaat gatgattacg
      781 atcggtctgt gcgtcgccaa tgcgctgacg ggactttccg gcggactgct cgcgcagtac
      841 cagaagtcct gtgatattaa ccttggaacc ggcatggtga cgatcgcgct cgccagtctg
      901 attatcggag agacgatcat cggaaagggc agcatgttaa agcgggtcat cggcgtgatt
      961 ctgggaagct gtctataccg ttttatcgtg gcggtggcgc ttcgcctgaa tgtcccggca
     1021 gagtgcttaa agctggtatc ctccattatt gtggcggtgg cgattgcttt cccttacctg
     1081 aagaagcagg cggtgttcta ccggcagaaa cgggcggcaa aagcggagaa tgagcagtat
     1141 attgcggaat tactgcgaga agaacggaag ggaaaaggag gaaatgcgtg atatggcagc
     1201 agaaagagaa tccatgctga ttatgaaaaa tgtcagcaag acctttaatc ctggcaccgt
     1261 caatgaaaag tgtgccctaa acggcgtcaa tctgacgctt ggcgccggag attttgtaac
     1321 gatcgtcggc agcaacggcg cgggaaaatc caccctgttc aatgcgatta ccggagcttt
     1381 tttcccggac cgcggtctga tcatgctcgg cggggaggat attacctatc agcaggagca
     1441 tgtgagaagc aaagtgatcg gtcatctgtt tcaggatccg ttaaagggaa cggcgccgca
     1501 catgacgatc gaggaaaaca tggcgcttgc ctatcttcgg acgacaacct cccggaatgc
     1561 atatttcagc aggatcaacg cggcggataa gaaaaaattc agagagcagc ttgcgctgct
     1621 tgacatggga ctggaagacc gtatgaagca gccggtggga cttctctccg gcggtcagcg
     1681 gcaggcactc acacttctca tggcgacgat ggtcacgccg aaaattcttc ttctggatga
     1741 gcacacggca gcactcgatc cggcgacggc agagaaggtg ctgcatctga ccagacagat
     1801 tgtggcggag cggcagatca cctgtctgat ggtgacgcac aacatgcatc aggcgctgac
     1861 gctcggcaac cggacattga tgatgaacgc gggaaatatt gtgctggacg ttgcgggaga
     1921 ggaacgtgcg gcgatgtcgg tggatgactt gttggagcag tttgcgctcc gcgcgggaaa
     1981 agcgctcgat aacgaccgta tcctattttc caaagtggag acacaggagt agggcgcagg
     2041 gagaaaaaca tcacatacag tccgaaacgg agtcggaata gcagatataa acagaa
//
LOCUS       DBMDQJ010000446         2089 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18306_length_2089_cov_1.543260, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000446 DBMDQJ010000000
VERSION     DBMDQJ010000446.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18306_length_2089_cov_1.543260"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="ACZO9R_08965"
     CDS             complement(<1..230)
                     /locus_tag="ACZO9R_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR0913228.1"
                     /translation="MSKIAESALDLIGNTPLLKLNRYSAEAGVKDATILGKLEYLNPA
                     GSVKDRIALAMIEDAEKKGILKPGATIIEPTSG"
     gene            complement(333..812)
                     /locus_tag="ACZO9R_08970"
     CDS             complement(333..812)
                     /locus_tag="ACZO9R_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HIR0913229.1"
                     /translation="MRKGTVRDMKKKVLQRGLFGIPTGIAIGYVITVMMSAFWGDGSY
                     YPCVPAFVDAMGSEIRAVVVQTVLCGLVGMVYAAGSVVWEVDRWSLAGQTAAYFLITL
                     GSVLPVAYAAYWMERSVAGVLGYVAIFCAIFAGIWIAQYLGWRAKINRINKKIALDK"
     gene            complement(809..1249)
                     /locus_tag="ACZO9R_08975"
     CDS             complement(809..1249)
                     /locus_tag="ACZO9R_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HIR0913230.1"
                     /translation="MKINIELDNALEETEVLIRAAKMTEEVNGILKKLEEPSMQMISG
                     FAGDTLEILEQADIIRIYAAKGKVYAVTAKGEYLLRMRLYEAEERLDQTRFVRISNSE
                     IINLKMAKRFDLSFSGTICVNLAGGQTAYVSRRYVAKIKRLLGI"
     gene            complement(1365..>2089)
                     /locus_tag="ACZO9R_08980"
     CDS             complement(1365..>2089)
                     /locus_tag="ACZO9R_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017043.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913231.1"
                     /translation="ELFQDLFLREEIKEILHSMYDFERIMSRVVYQNANARDLAALKN
                     SVENLPLLKKILSRCKSPYLSTLHDRLDTLENIHALIAQSIVEDPPFSVREGGMIREG
                     FNEELDTYTKAKENGTTWIHQLEEEEREKTGIKNLKVRYNKVFGYYIEVTKSNLELVP
                     EGYIRKQTLANAERFITPELKELEELIVKKAGLWLDSGDIFGAAGRGFERINVACPRS
                     VLTEALERLARVVREKEPIVCK"
BASE COUNT          465 a          635 c          419 g          570 t
ORIGIN      
        1 ccggaggtcg gctcaataat ggttgcgccc ggttttaaga tgcccttctt ctccgcatcc
       61 tcgatcatcg ccagtgcgat acgatccttt acggagcctg ccgggtttaa atactccagc
      121 tttcctaaga ttgttgcgtc ctttacgccc gcctccgcag aatagcggtt cagctttaat
      181 aaaggtgtgt ttccgatcag atccagtgcg ctctcagcaa ttttactcat cataaaatcc
      241 tcctgttctc aattcatttc cgtttctatg gtgtttttgt tttcctataa aactactatg
      301 ttttgtatga attgattata ggacgcgaaa tcttatttgt caagcgctat tttcttattt
      361 atacgattta tttttgcgcg ccatccaaga tactgggcaa tccagattcc ggcgaagatc
      421 gcacaaaaga ttgcgacgta gccaagcaca cccgcgacgc tgcgctccat ccagtatgcc
      481 gcatacgcca ccggaagcac cgagccgagt gtaatcagaa aatacgccgc cgtctgaccg
      541 gcaagactcc atcgatccac ttcccacacg accgatcccg ccgcatacac catgccgacc
      601 agaccacaca gcaccgtctg caccacaact gcccgtatct cactccccat cgcgtccaca
      661 aacgcaggca cacacggata gtagctgccg tcgccccaga atgccgacat catgaccgtg
      721 atcacatagc cgatcgcaat cccggttgga attccgaaca atcctctctg taacactttc
      781 tttttcatat ctctcaccgt tccctttctc atattcccaa cagccgcttg atctttgcca
      841 catatctgcg cgagacgtaa gccgtctgcc ctcccgcgag gttcacgcag atcgtacccg
      901 aaaaactcag atcaaaccgt tttgccattt tcagattgat gatctccgaa tttgagatcc
      961 gtacgaaacg cgtctgatcc agccgttcct ccgcctcgta aagccgcatc cgaagcagat
     1021 attcgccctt tgccgtcacc gcatagactt ttccttttgc cgcgtatatg cggataatat
     1081 ccgcctgctc cagtatctcc agtgtatctc ccgcaaaacc ggaaatcatc tgcatcgacg
     1141 gctcctccag tttttttagg attccgttga cttcctcggt cattttcgct gcccggatca
     1201 gtacctcggt ctcctccagt gcattgtcca gttcaatatt gattttcaca tgctcctcct
     1261 ctccgaaatg tcttcctctg ccggcatgtt ttcgtgtcct caagaactgt atttaacata
     1321 acaaaaatga ggtgcctttg caccccattt ttcataagtg gttcctattt gcacacgatc
     1381 ggttcttttt cccgcactac acgcgcgagc cgctccagcg cctccgtcag cacgctgcgc
     1441 ggacatgcca cattgatccg ctcaaagcct cttcccgccg cgccgaagat gtctccggaa
     1501 tccagccaca gtccggcttt ttttacaatc agctcctcca gctcctttaa ttcgggtgta
     1561 ataaaacgct ccgcattggc aagcgtctgc ttacggatat agccttccgg caccagctcc
     1621 agattggatt ttgtgacctc aatataatag ccgaacacct tattgtagcg caccttgagg
     1681 tttttaatgc ccgtcttttc gcgctcctcc tcctcaagct gatgaatcca tgtggtgccg
     1741 ttttccttcg ccttggtgta ggtatccagc tcctcattga agccctcacg aatcatgccg
     1801 ccctcgcgca cggaaaacgg agggtcttcg acgatggact gcgcaatcag cgcatggata
     1861 ttctccagcg tgtccagtct gtcatgcaga gtggacagat atggactctt gcagcgagaa
     1921 agaatcttct tcagcaacgg cagattttcc acggaatttt tcagcgccgc caaatccctt
     1981 gcgtttgcat tctgatacac cacacgggac ataatgcgtt caaagtcata catggaatgg
     2041 agaatttcct tgatttcctc tctgaggaac aggtcttgga acagctcct
//
LOCUS       DBMDQJ010000447         2089 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18307_length_2089_cov_1.336519, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000447 DBMDQJ010000000
VERSION     DBMDQJ010000447.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2089)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18307_length_2089_cov_1.336519"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="ACZO9R_08985"
     CDS             complement(<1..494)
                     /locus_tag="ACZO9R_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913232.1"
                     /translation="MKKIIGIVTPILLLILPYLLFVVLSVGMGRPDRDSSGAYIMAGD
                     SRLQMTQTAICIVYTVVVLLNVVWVIYQTAAGTGAQALLLWDMLMKLCYIPYYLLAAF
                     MVLAGFATFMFGGVAIIGVMWIADVLFLIPPSIYGLCGCIRACSEKKLSVGAAVALSI
                     LHFI"
     gene            complement(516..>1286)
                     /locus_tag="ACZO9R_08990"
     CDS             complement(516..>1286)
                     /locus_tag="ACZO9R_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR0913233.1"
                     /translation="KDEAIKGIWCVPKYSNPQGITYSDETVRRFARLKPAAKDFRIYW
                     DNAYCVHHLYDMDQDHLIEILAECKRAGNPDMVYKFCSTSKISFPGSGLAAIATSANN
                     LEDIKKQLKIQTIGHDKVNQLRHVRFFKDVHGITEHMRKHAASLRPKFEMILDTFDKE
                     LKDLGVGSWYSPKGGYFITYETLEGCAKSVVDKAKKAGVVMTPAGAPFPYGKDPKDSV
                     IRISPSYPSLEDLTTATQIFVVCVKLASIEKILGKQQA"
     assembly_gap    1287..1296
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1297..1809)
                     /locus_tag="ACZO9R_08995"
     CDS             complement(<1297..1809)
                     /locus_tag="ACZO9R_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HIR0913234.1"
                     /translation="MQSYQEMSKEELLKEKEHLEAEYKKFQQRGLKLDMSRGKPSQEQ
                     LDLSMGMMDVLSSYSDLACEDGTDCRNYGVLDGIQEAKVLIGDMIECNPENIIIYGNS
                     SLNIMYDTISRSMTHGVMGNTPWCKLDKVKFLCPVPGYDRHFAITEYFGIEMINAPML
                     PTGPDMDMVEE"
     gene            complement(1859..>2089)
                     /locus_tag="ACZO9R_09000"
     CDS             complement(1859..>2089)
                     /locus_tag="ACZO9R_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0913235.1"
                     /translation="TMTAPIPVHLQDAHYKSSQKLGHGVGYKYAHDYPNHYVKQQYLP
                     DGLTDRTFYHPSENGYEKTIREYFEKIKGMQP"
BASE COUNT          494 a          588 c          439 g          558 t
ORIGIN      
        1 aagataaagt gcagtatcga cagggcaact gctgccccta cggacagctt tttttcactg
       61 cacgcccgga tgcagccgca cagtccgtag atggacggcg gtatcaaaaa caggacatcc
      121 gcaatccaca tcacgccgat aatcgcgact ccgccgaaca taaacgtcgc aaatcccgcc
      181 agcaccataa acgccgccag cagataatac ggaatatagc acagcttcat cagcatatcc
      241 cacagaagca gcgcctgcgc gccggttcct gccgccgtct gatagatgac ccagacgaca
      301 tttaagagca cgaccaccgt gtagacaatg cagattgccg tctgcgtcat ctgcaatctg
      361 gaatcgcccg ccatgatata ggctccggac gaatctctgt ccggtcttcc catgcccaca
      421 gacagaacca caaaaagcag atacggtaaa atcagcagaa gaatcggtgt cactattcca
      481 attatttttt tcatatcatt ccctgctctg cgctcttatg cctgctgttt ccccagaatc
      541 ttctcaatgc ttgccagctt tacgcagact acaaaaatct gtgttgccgt tgtaagatcc
      601 tccagcgacg gatacgacgg agatatacgg atgacggaat ctttcgggtc cttgccgtac
      661 gggaacggtg ctcctgccgg tgtcatcaca acgcctgcct ttttcgcctt atctaccacg
      721 ctcttcgcgc atccctccag cgtctcatag gtaataaaat atcccccctt cgggctgtac
      781 cagcttccga cgccgagatc ctttaactcc ttgtcgaatg tgtccagaat catctcaaac
      841 ttcggtctga gggaagctgc atgctttctc atgtgctccg tgataccgtg cacatcttta
      901 aagaagcgca catggcgaag ctgatttacc ttatcatgac cgatcgtctg gatcttcagc
      961 tgcttcttaa tatcctccag attgttcgcg gaagttgcaa tcgcagccag accggaaccc
     1021 gggaagctga tcttgctggt cgagcagaac ttgtatacca tatccggatt gcccgcgcgc
     1081 ttgcactctg ccagaatctc gatcagatga tcctgatcca tatcatataa atgatgtaca
     1141 cagtaagcgt tatcccagta aatacggaag tcttttgccg ccggttttaa tctggcaaaa
     1201 cggcgtaccg tctcatccga gtaggtaatt ccctgcggat tggagtattt cggcacacac
     1261 cagattcctt tgatcgcctc atccttnnnn nnnnnnctcc tccaccatat ccatatccgg
     1321 tcctgtcgga agcattggag cattgatcat ctcaatgcca aaatattccg tgatggcaaa
     1381 atgtctgtca tagcccggaa ccggacataa gaacttcacc ttgtcaagct tgcaccacgg
     1441 cgtatttccc atcacaccgt gtgtcatgga gcgtgaaatc gtatcgtaca tgatatttaa
     1501 gctggaattg ccgtagataa tgatattttc cggattgcac tcgatcatat cgccaatcag
     1561 cactttcgcc tcctggattc cgtccagtac accgtagttg cggcagtccg ttccatcctc
     1621 gcatgcgaga tcggaatagg acgagagaac atccatcatg cccatagaca gatccagctg
     1681 ttcctgggat ggctttcctc tggacatgtc aagcttcaat ccccgctgct ggaacttctt
     1741 gtattctgct tccagatgtt ccttctcttt taacagttcc tctttgctca tctcctgata
     1801 tgactgcatt gttccttcct cctgctatct gtatatacat tttatatgaa ccttatttct
     1861 aaggctgcat cccttttatt ttttcaaaat attcccggat tgttttctca tagccgttct
     1921 ccgacggatg ataaaacgtg cggtcggtca gaccgtccgg cagatactgc tgcttcacat
     1981 aatgattcgg ataatcatgc gcgtatttgt aaccgacgcc gtgtcccagc ttctgtgatg
     2041 acttataatg tgcatcctgc aggtgcaccg gtatcggtgc cgtcatggt
//
LOCUS       DBMDQJ010000448         2083 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18370_length_2083_cov_1.557013, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000448 DBMDQJ010000000
VERSION     DBMDQJ010000448.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18370_length_2083_cov_1.557013"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(133..>2083)
                     /locus_tag="ACZO9R_09005"
     CDS             complement(133..>2083)
                     /locus_tag="ACZO9R_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR0913236.1"
                     /translation="NVDEITSGQVKFLNPFMSFASEETLSSQLIKTFGQSEFGIPESE
                     IRDAVAEGWKELAVCRMEMQKKGEEVLQYLKETGRRGIVLAGRPYHIDPEINHGIPEL
                     ITSYGIAVLTEDSISHLQPVERPLIVMDQWMYHSRLYAAANYVKTRDDLDLIQLNSFG
                     CGLDAVTTDAVNDILTKSGKIYTCLKIDEVNNLGAARIRIRSLISAIRVREKKQTKRT
                     IVPANYNRVVFTEEMRKNYTILCPQMSPIHFELLEPAFQTAGYNLVVPDVDSRTCVDV
                     GLKYVNNDACYPSLIVVGQLMAAVMSGGYDMSRTAVLISQTGGGCRASNYIGFIRRAL
                     EKAGYPDVPVISINLSGLEKNPGFKLTLPLIQHGLYALEFGDIFMRCVYATRPYEAVA
                     GSTDELHEKWKKEVIAFITQKKMLSHGKFKKMCREIIRDFDNLPRKDIKKPRVGVVGE
                     ILVKFHPAANNHLVELLESEGAEAVVPDLTDFLLYCFYNTGFKADNLGMSQKSKKIGR
                     LGINFFEWLRSAARDEFTKSRHFTAPAHIDDLARYARDIVSEGNQTGEGWFLTGEMLE
                     LIHTGTPNIVCTQPFACLPNHVVGKGVIKELRHRYPGSNIVAIDYDPGASEVNQLNRI
                     KLMLSTANKNLAKQNAPEQKDQAAG"
BASE COUNT          448 a          561 c          565 g          509 t
ORIGIN      
        1 aaatgccaca ttttttgaac caaatttccc tctgtatctc catctgaaat cccacggtgt
       61 gatcttctga taatattact tatcacgaaa aggggctgca catttgatgc gcagcccctt
      121 tctctgtatc tgtcagcctg cagcctgatc cttctgctcc ggtgcgttct gctttgccag
      181 atttttgttc gcggtggaga gcatcagttt gatacggttg agctggttta cctcgctggc
      241 acccggatcg taatcgatgg ccacgatatt ggaacccgga tagcggtgac gcagttcctt
      301 gatgacaccc ttgccgacca catggttcgg caggcaggcg aacggctgcg tgcagacgat
      361 gttcggtgtt ccggtgtgga tcagctctaa catctcaccg gttaagaacc agccctcgcc
      421 ggtctggttg ccctcggata caatgtctct tgcatatctg gcaagatcat caatgtgcgc
      481 aggtgcagta aagtgcctgc tctttgtgaa ttcatctctt gcagcgctgc gcagccactc
      541 aaagaaattg atgcccagac ggccgatctt cttggacttc tggctcatgc cgagattgtc
      601 tgctttaaag cccgtattgt agaagcagta gagcaggaaa tcggtcagat ccggtacaac
      661 ggcctctgca ccctcggatt cgagaagctc caccagatga ttgttggcag ccggatggaa
      721 cttgacaaga atctcgccga caacgccgac gcgcggtttc ttgatgtctt tgcgcggaag
      781 gttgtcaaaa tcgcggatga tctcgcggca cattttcttg aacttgccgt gggagagcat
      841 ctttttctgg gtaataaagg cgataacctc ttttttccat ttttcgtgca actcgtccgt
      901 tgatccggca accgcctcgt aagggcgcgt tgcgtagacg cagcgcatga agatatcgcc
      961 aaactccagc gcgtagagtc cgtgctggat cagtggcagt gtcagcttaa agcccgggtt
     1021 tttctccaga ccgctcagat tgatcgagat gaccggaacg tccggatagc cggctttttc
     1081 cagtgcacgc cggatgaagc cgatgtaatt gctggcgcgg cagccgccgc cggtctggga
     1141 gattaagacc gcggtgcgcg acatatcata accgccggac atcacggccg ccatcagctg
     1201 gccaaccacg atcagggacg ggtagcaggc gtcattgttg acgtacttta agccgacatc
     1261 cacgcaggtg cggctgtcca catccggaac aacaaggttg tacccggccg tctggaacgc
     1321 cggctccaac agctcaaaat ggatcggtga catctgtggg cagaggatgg tgtaattctt
     1381 gcgcatttcc tccgtgaaga cgacgcggtt gtaattcgcc ggaacgatgg tgcgcttcgt
     1441 ctgcttcttc tcacggacac ggatcgccga gatcagggaa cggatacgga tgcgcgccgc
     1501 accgaggttg ttgacctcat cgatttttaa acaggtgtag atcttgccgg actttgtcag
     1561 aatatcattg acagcgtctg tcgtgacggc atccagacca cagccgaagg aattcagctg
     1621 gatgaggtcg agatcgtctc ttgtcttgac atagttcgcc gctgcgtaca gtctggagtg
     1681 atacatccac tgatccatga cgatgagtgg gcgctccacc ggctgtaagt gggagatgga
     1741 atcctccgtg agcacggcga tgccgtagga ggtaatcagc tccggaatac cgtggttgat
     1801 ctcagggtct atatggtacg gacgacctgc gaggacgata ccgcgtcttc cggtctcctt
     1861 taaatactgt aagacttcct cgcctttttt ctgcatctcc atccggcaga cggcaagctc
     1921 cttccagcct tcggcaaccg cgtcgcggat ctcggattcc ggaatcccga actccgactg
     1981 gccaaaggtc ttaatcagct gggaggaaag cgtctcctcg ctggcgaaag acataaacgg
     2041 atttaagaac ttcacctgac cggaagtgat ctcgtccacg tta
//
LOCUS       DBMDQJ010000449         2067 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18549_length_2067_cov_1.378942, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000449 DBMDQJ010000000
VERSION     DBMDQJ010000449.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18549_length_2067_cov_1.378942"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="ACZO9R_09010"
     CDS             <1..392
                     /locus_tag="ACZO9R_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913237.1"
                     /translation="ADGFDVHNIFRTSICREDLLLLDSLEYNLSATAAFSQYGLHAAT
                     LPDSVRLLHYTGEKPWHGDHLHNDLESLWWNYAKKSPYYPALLEQTIQGMILGTNVIT
                     YISNIQKEHRQLTAIVDQYQTLLEKLS"
     gene            complement(384..1079)
                     /gene="pseF"
                     /locus_tag="ACZO9R_09015"
     CDS             complement(384..1079)
                     /gene="pseF"
                     /locus_tag="ACZO9R_09015"
                     /EC_number="2.7.7.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007882674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudaminic acid cytidylyltransferase"
                     /protein_id="HIR0913238.1"
                     /translation="MEKAIAIITARGGSKRIPGKNIKNFCGKPIIAYSIEAALKSGLF
                     SEVMVSTDSQKIAQIAQEYRASVPFMRSAKTSDDYATTSDVIMEVMDNYKKLGRTFEY
                     ICCIYPTAPFVTAQKLIAAQTIMEEKHPVMVMPMTAFSYPPQRCYVMNDDGFMGYKYP
                     QYSRTRSQDLEKQYHDAGQFYVYNAEKYLALHGQVNEGVMPIIVSELEVQDIDNEDDW
                     KIAELKYQLMASR"
     gene            complement(1101..>2067)
                     /locus_tag="ACZO9R_09020"
     CDS             complement(1101..>2067)
                     /locus_tag="ACZO9R_09020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR0913239.1"
                     /translation="KKGRAPFYHMQEAEYAAELSELSSLEFKGETFSYMQCPEDMPDL
                     QVIASLVQLIRDRRPDYIIGIGGNDICADICGRIVPQITVSTVFSGIATSCGEWQMVG
                     KRTLSGTDEMRLQELGVQKECVKCVPFTFSFKPQQDHYTRADLTLPEEKFLVATVGWR
                     LDDEVTGAYLKMLEEAVGEEPAIHVVFVGRFHRFGEALAGYPVLQAHATNLGEQQDVL
                     AVLECMDLYVNPARSGGGSSAAEALSKGLPVVTLPVGDVAVAAGEIFEVEDYPAMRRQ
                     ILRYAADHRLYAEMTQKARARAELLLDSKAGFGQALMEIEQQLES"
BASE COUNT          490 a          594 c          401 g          582 t
ORIGIN      
        1 tggcagacgg ctttgatgtc cacaacatct tccgtacatc catttgccgg gaagatcttt
       61 tactgctgga ttcgctggag tataatctgt ctgcgactgc tgccttttcc caatacggtc
      121 tgcatgccgc cactctgccg gacagtgtcc ggcttctcca ctatacgggt gaaaagccct
      181 ggcacggcga tcatctgcac aatgacctgg aatctctctg gtggaactat gcaaaaaaat
      241 ccccgtatta tccggctctt ctggaacaga caatacaggg gatgattctg ggtaccaatg
      301 ttatcaccta catctccaat attcaaaaag agcatcggca gctgaccgct atcgtcgatc
      361 agtaccagac gctcctcgaa aaactatctt gatgccatca gttgatattt taattccgca
      421 atcttccagt catcttcgtt atcaatatcc tgtacttcaa gttcggagac aatgattggc
      481 atcacacctt cgttcacctg tccgtgaagt gcaaggtatt tttccgcatt ataaacataa
      541 aactgtccgg catcatgata ctgtttttcc agatcctgcg accgggttct gctatactgc
      601 ggatatttat agcccataaa cccatcatca ttcatgacat aacatctctg tggcggatag
      661 gaaaacgccg tcatcggcat taccattaca ggatgttttt cttccataat cgtctgtgcc
      721 gcaatgagct tttgtgccgt cacaaagggc gcagtcggat aaatacagca gatatattca
      781 aacgttctcc ccagtttttt ataattgtcc atgacttcca taatgacatc cgatgttgtt
      841 gcatagtcat ccgatgtctt cgcgctgcgc ataaacggta cacttgccct atactcctgc
      901 gcaatctgtg caattttctg actgtccgta gacaccatta cttccgaaaa aagtccactc
      961 tttaacgccg cctctatcga gtatgcgata atcggtttgc cgcagaaatt cttgatgttt
     1021 tttcccggaa ttcttttgct gcctcctctg gcagttataa ttgcaatcgc cttttccata
     1081 tttcttccct cctggtcaaa ctatgattcc agctgttgtt ctatctccat gagtgcctgt
     1141 ccgaatccgg ctttgctgtc cagcagcagt tctgcccgcg ctctggcttt ctgtgtcatc
     1201 tccgcataca acctgtggtc tgcggcatag cgcagaatct gtctccgcat ggcaggataa
     1261 tcttccacct cgaatatctc tcccgccgcg accgccacat cccctacagg aagtgtcacc
     1321 accggcaagc cctttgagag tgcttctgcc gccgaacttc caccacccga tctggctggg
     1381 tttacataca gatccataca ttccagtacc gcaagcacat cctgctgttc tccaagattc
     1441 gtcgcatgtg cctgtagaac aggatatccg gcaagcgcct ccccaaaacg gtgaaacctg
     1501 cctacgaaca ctacgtgtat cgccggttct tctccgactg cctcctccag cattttcaga
     1561 tacgccccgg taacttcatc atccagtcgc catcccacag tcgcaacaag aaatttttct
     1621 tccggcaagg taagatctgc tctggtgtaa tgatcctgct gcggcttaaa cgaaaaagta
     1681 aacggaacac atttgacgca ttccttttga acacctaatt cctgcagccg catctcatcc
     1741 gtcccggaaa gcgtcctctt gcctaccatc tgccactcac cgcatgaagt cgctattccc
     1801 gaaaacacgg tgctgactgt aatttgtggt acaattcttc cacaaatatc tgcacagata
     1861 tcattcccgc cgattccaat gatgtaatcc ggacgtctgt cacgaatcag ctgcacaagt
     1921 gacgctatca cctgcagatc cggcatatcc tctggacact gcatatagga aaatgtttct
     1981 cccttaaatt ccagcgacga aagctctgac aattccgctg cgtattctgc ctcctgcata
     2041 tgataaaatg gtgcgcgtcc ttttttt
//
LOCUS       DBMDQJ010000450         2054 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18687_length_2054_cov_1.356887, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000450 DBMDQJ010000000
VERSION     DBMDQJ010000450.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18687_length_2054_cov_1.356887"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="ACZO9R_09025"
     CDS             <1..931
                     /locus_tag="ACZO9R_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR0913240.1"
                     /translation="NFEEYPNAIDPTVFYDADDRMGMVYGSWSGGIYLLEIDPATGQV
                     IHPEADEEHNVDAYYGKKLLGGGHKSIEAPYILYDETTGYYYLYVSYGTLTSSGGYQV
                     RVFRSKTVDGDYVDMNGKYPTADVDHQLYGLKLTGNYKLPSLERAYMATGHNSAFIDE
                     DGRQYLVYHTRFNNGTENHSPRVHQMMMNEDGWPCELPYQTQGETASDTGYETDAVVG
                     RYFVINQGTDISNDIANPVILYLNADGTVVGREAAGTWEAVDGTYYMHITIDGEDYSG
                     VFCQMKDEAGTDVMTFSAVGGNQSVWGVKYIAE"
     gene            993..1955
                     /locus_tag="ACZO9R_09030"
     CDS             993..1955
                     /locus_tag="ACZO9R_09030"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HIR0913241.1"
                     /translation="MEKQLKYNEPWILQRADPYVYCHTDGTYYFTASVPEYDRIVLRR
                     AALLADLADAEEHEVWHKHESGPQSIHIWAPELHYLFGKWYLYYAGGDKDDIWAIRPY
                     VLECQGDDPVHDPWVEKGKMTRADGDEFSFEAFSLDATVFEVNGSWYYIWAEKVGVGR
                     QISNLYIARMKNGYTLDSVQVLLTTPDYDWERHGFWVNEGPAVIQKDGKVFMTYSASD
                     TGVHYCMGMMTADADADLLDPLSWKKERYPVLTSSEKAGIYGPGHNSFTKDEAGDDIM
                     VYHARTETEIVGNPLYNPNRHAMLMKIRWQDDRPVFGFDEQDCR"
BASE COUNT          542 a          449 c          622 g          441 t
ORIGIN      
        1 taactttgag gaatatccaa atgcgatcga cccgacggta ttttatgatg ccgacgaccg
       61 tatggggatg gtgtatggtt cctggagcgg tggcatctat ctactggaaa ttgatcccgc
      121 gaccggtcag gtgatccatc cggaggcaga tgaagaacac aatgtggatg cgtattatgg
      181 gaaaaagctg ctcggcggtg gacataaatc tatcgaagcg ccgtacattc tctatgacga
      241 gacgaccgga tactattatc tgtatgtttc ctatggtacg ctcacgagca gcggcggtta
      301 tcaggtgcgc gtgtttcgtt ccaagaccgt ggacggcgat tatgtggaca tgaacgggaa
      361 atacccgact gcggatgtgg atcatcagct ttacggtctg aagctgaccg gcaattacaa
      421 gcttccgtcc ttggaacgcg cgtacatggc gaccggacat aactctgcgt tcatcgacga
      481 ggacggcaga cagtatctgg tctaccatac ccgttttaac aacggcacgg aaaatcattc
      541 gccgcgcgtg catcagatga tgatgaacga agacggatgg ccgtgcgaac tgccgtatca
      601 gacacaggga gagacggcga gcgataccgg ttatgagacg gatgcagtgg tcggaagata
      661 ttttgtgatc aaccagggaa cggatatcag caatgacatt gccaacccgg tgatcctgta
      721 cttgaatgct gacggtacag tcgtcggaag agaggctgcg ggaacctggg aagctgttga
      781 cggaacgtac tatatgcaca ttacgattga cggagaggac tacagcggcg tgttctgcca
      841 gatgaaagac gaggcaggca ccgatgtgat gacattttct gccgtgggcg gaaaccagag
      901 cgtctggggc gtaaaatata tcgcggaata aaacagaata aaaagaaaac gtccggcaag
      961 ggctcagccg gatttgttat aaggagaaaa agatggagaa acagttgaaa tataatgagc
     1021 cgtggattct gcagagggca gatccttatg tgtactgtca cacggacgga acctactatt
     1081 ttaccgcatc ggtgccggaa tacgacagga ttgttctgcg ccgtgccgca cttcttgcgg
     1141 atctggcgga tgccgaagag catgaggtgt ggcacaagca cgagagcgga ccgcagagca
     1201 tccatatctg ggcgccggaa ctgcattatc tctttggaaa atggtatctc tactatgccg
     1261 gcggcgataa ggacgacatc tgggcgatcc gcccgtatgt gctggaatgc cagggtgatg
     1321 acccggtaca tgatccgtgg gtggaaaaag gcaagatgac ccgtgcagat ggagacgaat
     1381 tctcgtttga ggcattttcc ctggatgcga ccgtcttcga ggtaaacgga agctggtatt
     1441 acatctgggc ggaaaaagtg ggagtcggca ggcagatttc caatctttac atcgccagaa
     1501 tgaagaacgg ctacacgctg gattccgtgc aggttcttct gacaacgccg gattacgact
     1561 gggagcgcca cggcttctgg gtaaatgagg gaccggcggt cattcagaag gacggcaaag
     1621 tatttatgac gtattccgcc agcgataccg gcgtgcatta ctgcatgggt atgatgacgg
     1681 cggatgcaga cgcagatctg ttagatccgc tctcctggaa aaaagagcgc tatccggtac
     1741 ttacatccag tgaaaaagcg ggaatctacg gcccgggaca taattccttc acgaaggatg
     1801 aggcaggaga tgacattatg gtctaccatg cgagaacgga gacggagatt gtgggcaacc
     1861 cgctgtacaa tccgaaccgt catgcaatgc ttatgaagat cagatggcag gacgaccgac
     1921 cggtgttcgg gttcgacgag caggattgcc ggtaaaaatt cagaaatgtg ggcatgagat
     1981 atggagacga gagtgtgtac gcgctgtctg cttcgggata tgatcgaggc gggggacagt
     2041 atggaaatga ttga
//
LOCUS       DBMDQJ010000451         2051 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18721_length_2051_cov_1.453846, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000451 DBMDQJ010000000
VERSION     DBMDQJ010000451.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18721_length_2051_cov_1.453846"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            98..946
                     /locus_tag="ACZO9R_09035"
     CDS             98..946
                     /locus_tag="ACZO9R_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR0913242.1"
                     /translation="MTPLHSQILTARDRNGQELRTIQKQLQKLPPGKLICSRNGTTVK
                     WYQGDGHSKTYIPKANRTLAEQLALKKYLQQHEQILLQEQAAITAYLKYSPAGSLSSD
                     DPHLLSDPACENLLHSYFSTSKNIPDVSNVSDISDISTRSAVEWSAASYERNPLHPEQ
                     LLHKTVSGERVRSKSEAMIAMLLHLNAIPYRYECALSLGGITLFPDFTIRHPGTGALY
                     YWEHFGLMDDPAYAKNAGSKLSLYAANGIIPSVHLITTYETKAYPLNAGMIEHLISYY
                     FLDSAV"
     gene            complement(1096..1560)
                     /locus_tag="ACZO9R_09040"
     CDS             complement(1096..1560)
                     /locus_tag="ACZO9R_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVA/SpoVAEb family sporulation membrane
                     protein"
                     /protein_id="HIR0913243.1"
                     /translation="MDKEKERLQKKYNDYVKQVTPTHNLWWNMAKAFVTGGIICCIGQ
                     FILNVCENYGLDQKTSGSWCSMLLILLSVLLTGLNLYPSIANWGGAGALVPITGFANG
                     VAAPAIEFQKEGQVFGIGCKIFTIAGPVILYGIFSSWVLGLIYWVLKWWGMV"
     gene            complement(1666..2010)
                     /locus_tag="ACZO9R_09045"
     CDS             complement(1666..2010)
                     /locus_tag="ACZO9R_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3837 domain-containing protein"
                     /protein_id="HIR0913244.1"
                     /translation="MVPSIARQSVIIKCNMQKSILTGNYEFYYAAGLIAKLSRVEIPE
                     DIKPEELLALLSEQIPMLTPADEKEKYLFGMVADYRPEDVYDEQMRELLDWGRTEKYL
                     WTVTLPDDWQNA"
BASE COUNT          491 a          625 c          395 g          540 t
ORIGIN      
        1 tttcccgtct acacttaaaa cagcaaaggt catttttagc acataaaaat aaaccggatc
       61 ctcttaacac acaccatcta tagaatagga gaataccatg acacctttac actcacaaat
      121 cctcactgcc cgcgaccgca acgggcagga actccgtacc atccagaagc agcttcagaa
      181 gctccctccc ggaaagctca tttgctcccg caacggaacc accgtcaaat ggtatcaggg
      241 cgacggtcac tccaagacct atattccgaa agcgaaccgc actctcgccg aacagcttgc
      301 gctgaaaaag tatctgcagc aacatgagca aatactcttg caggagcagg cagcaatcac
      361 tgcctattta aaatactccc ccgcgggcag cctctcttcc gatgatccac atcttctctc
      421 tgatcccgcc tgtgaaaatc tgcttcattc ttatttttcc acctcaaaaa atattccgga
      481 cgtctcgaat gtttcagata tctcggatat ttcgactcgt tccgctgtcg aatggtctgc
      541 cgcttcctac gaacgcaatc cgctccatcc cgagcagctg cttcacaaaa ccgtctccgg
      601 cgagagggtg cgttccaaat cagaagccat gattgccatg ctgctccacc taaacgccat
      661 tccttaccgc tacgaatgtg cgctctccct tggcggcatc actctgtttc cggatttcac
      721 catacgtcat cccggaaccg gcgcactcta ctactgggag cattttggtc tgatggacga
      781 tcccgcctac gcaaaaaacg ccggctcgaa gctttctctc tatgccgcaa acggcatcat
      841 tccatccgtt catctgatca ccacgtacga gaccaaagca tatcccttaa acgccggcat
      901 gattgagcat ctgatttcct actattttct ggattccgcg gtctgaatcc gtgcttccct
      961 cttccactgt cacttcctgc ttttcgttcg cttctcgggt ctggaacggc gtttttcctc
     1021 tctcagaccc gaaaatcaca atatttcggg tctgggagaa cgtttttcct cttccagacc
     1081 cgcacggaaa aggcactaca ccattcccca ccattttaat acccaataga tcaatccgag
     1141 cacccagctt gaaaatattc cgtataaaat gactggtccg gcaatcgtaa agatcttgca
     1201 gccaattccg aagacctggc cctctttctg aaactctatg gcgggcgcgg ccacaccatt
     1261 tgcaaatccg gtaattggca ccagcgcgcc cgcgcctccc cagtttgcaa tcgaagggta
     1321 cagattcaga ccggtgagca gtacagacag cagaatgagc aacatagagc accagcttcc
     1381 agatgttttc tgatccagtc cgtaattctc acacacgttc agaataaact gcccgatgca
     1441 gcagatgatc ccgcctgtca caaacgcctt cgccatattc caccagagat tatgggtggg
     1501 cgtcacctgc ttcacataat cattgtattt tttttgtaaa cgttcttttt ccttatccac
     1561 tgcgcaacct ccgtcttttt tgtcagtatg cgcattttat ttatttctat tcctccgacc
     1621 gctcctcccg ccatttctcc tctgtgatgc tatgctgctc tacctttatg cgttctgcca
     1681 gtcatccggc aatgtgaccg tccagagata tttttcagtg cgtccccagt ccagaagttc
     1741 ccgcatctgc tcatcatata catcctcggg gcggtagtcc gccaccatcc cgaacaggta
     1801 tttttctttc tcatccgccg gtgtcagcat cggtatctgc tccgacagga gcgcgagcag
     1861 ttcctccggc ttgatatcct cggggatctc gactctggaa agcttggcga tgagtcccgc
     1921 agcataatag aattcataat ttcctgtcaa aatcgatttc tgcatattgc acttgataat
     1981 cacgctctgg cgtgcgatgg atggcaccat aattttcctc cgtaaaagaa caatctccta
     2041 tcattgtact a
//
LOCUS       DBMDQJ010000452         2044 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18803_length_2044_cov_1.544519, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000452 DBMDQJ010000000
VERSION     DBMDQJ010000452.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18803_length_2044_cov_1.544519"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(63..1319)
                     /locus_tag="ACZO9R_09050"
     CDS             complement(63..1319)
                     /locus_tag="ACZO9R_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HIR0913245.1"
                     /translation="MNRYMITREKIKGQEYLISALYDEKKHMIEVLPEAAGETSLLGN
                     IYVGKVENIVTNLNAAFVKIAPDIRCYLPLSDVKNPVFTRKLSQTKALAAGDELLVQV
                     SREALKTKDPAVTTNLTIAGTYAVLTSGNRKKSVSSKVKGEARERLVKLVEQTAGEYG
                     VILRTNAAEASDETVLLELEVLLERYETMAEAAAHKNCYTLVYREEAGWLRHIRDLRK
                     DDLQEIVTDDRGMFEDICSACRIHKQALTTGGSVPVPVDDVMTADGIRIRYYKDPALS
                     LGALYSVRSELKNALSERVWLKSGAYLVIQPTEALTVVDVNTGKNVAKKEMQENFLRV
                     NKEAAEEIARQLRLRNLSGMVLVDFINLTSKSAEEELLKTFRAALQKDPVPAQLIDMT
                     ALGLVEVTRKKIKRPLHEIFPDGLGF"
     gene            complement(1316..2032)
                     /locus_tag="ACZO9R_09055"
     CDS             complement(1316..2032)
                     /locus_tag="ACZO9R_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HIR0913246.1"
                     /translation="MKIRIRFRKYGVMKFIGHLDVMRYFQKAMRRAHIDICYSEGFSP
                     HQIMSFAAPLGVGITSDGEYMDIEVHTSASTEESLRALNGVMVEGVECTGYVKLPDQA
                     KTAMSIVAAADYDLYFKEGYAAPVSVSAFADGIRDFFEKPDEVLITKQTKKSERVLDI
                     KPLIYGFQVTDRGGVPSFYLKLSTGSTDNLKPELVLEALFLHMGLTYDPNAIQIHRKD
                     VYASDGDDFVSLGAMGEVIE"
BASE COUNT          458 a          611 c          450 g          525 t
ORIGIN      
        1 ttattctaag gaaaaaaaca ggcacaaaaa aagatacctt ttttttaaaa ggtatctttt
       61 ttttagaatc ccagaccgtc cggaaaaatc tcatgcagcg gtctttttat ttttttgcgg
      121 gtcacctcga ccagaccgag tgcggtcata tcgataagct gcgccggaac cggatctttt
      181 tgcagtgccg cccggaatgt cttaagcagt tcctcttccg cggattttga tgtcagattt
      241 ataaaatcca ccagaaccat tcccgagaga ttccgcagcc gcagttgtct ggcaatctcc
      301 tctgccgctt ccttgttcac gcgcagaaag ttctcctgca tctccttttt tgccacattc
      361 tttccggtgt tgacatccac caccgtcaga gcctccgtcg gctgaatgac cagataggca
      421 ccggatttta accatacgcg ctcactcaat gcatttttca gctccgaccg gacactgtag
      481 agtgctccaa gggagagcgc cggatccttg taataacgga tccggattcc atccgccgtc
      541 attacatcgt ccaccggcac gggcacgctt cctcccgtcg taagcgcctg cttatggatc
      601 cggcaggcac tgcagatatc ctcgaacatt ccgcggtcat ctgtcacaat ctcttgcaga
      661 tcgtccttcc gcaggtctct gatatggcgc agccatcccg cttcctccct gtatacaagc
      721 gtgtaacagt tcttgtgtgc cgccgcctcc gccatcgtct catagcgctc caaaagcact
      781 tccagttcaa gcagaaccgt ctcatcggaa gcctctgcgg cattggtgcg gagaatcacg
      841 ccgtactccc ctgccgtctg ctctacaagc ttcaccagac gctctctggc ctcccccttt
      901 acctttgatg agacgctctt cttccggttt ccggaagtta agacggcata ggttcccgcg
      961 atcgtcaggt ttgtcgtcac cgcgggatct tttgtcttaa gcgcctcgcg cgagacctgt
     1021 accagaagtt catcgcccgc cgcaagcgcc tttgtctgcg acagctttct ggtaaaaacg
     1081 ggattcttca catcggacag cggcagatag caccgtatat ccggcgctat cttgacaaac
     1141 gcggcattta gattggtgac aatgttctcg acttttccga catagatatt gccgagcagt
     1201 gatgtctccc ccgccgcctc cggcaatacc tcaatcatat gttttttctc atcataaagg
     1261 gcagaaatca gatattcctg cccttttatc ttctctcgcg ttatcatgta acggttcatt
     1321 cgattacttc tcccatcgcg cctaaggaca caaaatcatc cccgtcggac gcatagacat
     1381 ccttcctgtg aatctgaatc gcattgggat cataggtaag tcccatgtgt aaaaacagcg
     1441 cctcaagcac cagctccggc ttcagattat ccgtacttcc tgtggaaagc ttcagataga
     1501 aggacggcac gccgccgcgg tccgtcactt gaaagccgta gatgagtggt ttgatatcca
     1561 gcacgcgctc gctctttttc gtctgcttgg tgatgagaac ctcatccggt ttctcaaaga
     1621 aatcgcggat accgtcagca aacgccgaaa cagaaaccgg tgccgcatac ccttccttaa
     1681 aatacagatc ataatctgcg gcggcaacga tcgacattgc cgtctttgcc tgatccggaa
     1741 gtttcacata accggtgcac tccacgccct ccaccatgac gccgtttaac gcccggaggg
     1801 attcctccgt ggatgcactc gtgtgcactt caatgtccat atactcgccg tcgctcgtga
     1861 tgcccacgcc gagcggcgca gcaaacgaca tgatctggtg cggggaaaat ccctccgaat
     1921 agcagatgtc aatgtgtgct ctgcgcattg ccttctggaa gtaccgcatc acatcgagat
     1981 gtccgataaa cttcatcaca ccgtacttgc ggaaacggat tctaattttc atagcagaca
     2041 cctc
//
LOCUS       DBMDQJ010000453         2032 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18930_length_2032_cov_1.332988, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000453 DBMDQJ010000000
VERSION     DBMDQJ010000453.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18930_length_2032_cov_1.332988"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="ACZO9R_09060"
     CDS             <1..657
                     /locus_tag="ACZO9R_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025314.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR0913247.1"
                     /translation="DWSNKLYNGTTTGATTAWNFFFSPEFGNKNTTNEQYVNILYQAL
                     FDRNADTDGKANWVSLLDNGVSRAYVLKGFTDSSEFNNLCSVYGIQQGTITTSENRDK
                     NPQVTAFVNRLYTIAMNRAADVNGLNDWTGKLLAKTKTPKQVASGFVFSQEMANRNLS
                     NQEYVTMLYRTMMDREPDEAGLNDWVTRLQSGTSRQSVFDGFADSTEFDNIVRAYGLK
                     "
     gene            903..>2032
                     /locus_tag="ACZO9R_09065"
     CDS             903..>2032
                     /locus_tag="ACZO9R_09065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013357.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HIR0913248.1"
                     /translation="MKKRVLACILAAALLTTGIPGGQAAMAQSLTENGTEMATEEVNP
                     ENTSEETEAASVTETEVQTSTERETEDVAEGSESQLTVTEETEAAEETEAAEETEAAE
                     GTGKTEETEETEAVEKTGHLKASGTVAEEALEEDPQAGTSMSNEEPESTSNIKSSSAT
                     YSGYTGSSYIHNGRYDSGYKIVNGIDVSYHNGDINWSAVKAAGIDYALIRVGYRGMSN
                     GGLFDDSKYRANIQGALNAGLRVGVYIFSQATTQAEAAEEANYLLNRISGYNITLPVV
                     IDYEFGTNHSGRLADANLDIDTATAVVNAFCTTVQSAGYTPMVYANKTMLQSYIRGEI
                     LDDYYKIWLANYTTQTTYAGEYYAWQYSSKGGVSGISGYVDC"
BASE COUNT          619 a          411 c          596 g          406 t
ORIGIN      
        1 gactggagca acaagctgta caacggaacg acgaccggtg cgacgactgc atggaatttt
       61 ttcttcagcc cggagttcgg caataaaaat acaacaaacg agcagtatgt gaacattctg
      121 tatcaggcgc tgtttgaccg caatgcagat acggatggca aggcaaactg ggtgagctta
      181 ctggataacg gagtgtcccg tgcgtatgtg ctgaaaggat ttaccgattc gtcagagttc
      241 aacaatctct gcagcgtgta tggaattcag cagggaacca taacgacgtc cgagaaccgc
      301 gataagaatc cgcaggtaac ggcatttgtg aaccgcttat atacgatcgc aatgaaccgt
      361 gccgcagatg tcaacggtct gaatgactgg acgggcaagc tgctggcgaa gacaaagaca
      421 ccgaagcagg tggcatccgg attcgtattt tcacaggaga tggccaaccg gaatctcagt
      481 aatcaggagt atgtgacgat gctttaccgt acgatgatgg acagagagcc ggatgaggca
      541 ggcctgaatg actgggtaac cagactgcag tcaggtacca gccgccagag cgtatttgac
      601 ggttttgccg acagtacaga gtttgacaac attgtacgtg catacggatt aaagtaaata
      661 atacgtcccg gtaggacata cagtttcata cagaggaaga aagagcccgc gctgtcgatt
      721 tgacagcgcg ggctcttttg tataaaggaa acgttgctct cacagacagg tctgtcagat
      781 tctggggaag aatgaccgga aaaattaagg aaatatacat attttgtaaa ttgacgttgt
      841 ccacggtacg tgtataatta aaattggtag aaatatgtta tttagacaga ggaaggatca
      901 agatgaagaa aagagtacta gcgtgtatac tggcggcagc gcttctgaca accggaatac
      961 cgggaggaca ggcagcgatg gcacagagcc ttacggaaaa cggaacggag atggcgacag
     1021 aagaagtaaa tccggaaaac acatcggagg aaacggaagc ggcatctgtg acggaaacag
     1081 aagtgcaaac gtcgacagag agagagacgg aggatgtggc agaaggctcg gagagtcagt
     1141 tgacagtgac agaggagacc gaagcggcag aggagaccga agcagcagag gagaccgaag
     1201 cggcagaggg aaccgggaag acagaggaga ccgaagagac ggaagcagta gagaagacag
     1261 ggcacttaaa ggcatccggc actgtggcag aggaagcgtt ggaagaagac ccgcaggcgg
     1321 gtacctccat gagcaatgag gagccggaga gcacatcgaa tatcaaaagc tcatcggcga
     1381 cttacagtgg atataccgga agctcctata tacataacgg gcggtacgac agtggttata
     1441 agattgtgaa cggaatcgac gtttcttatc acaatggaga tattaactgg tcggcggtca
     1501 aggcagccgg aattgactac gcgctgatcc gtgtgggata ccgcggaatg tcgaacggag
     1561 gattgttcga tgattccaag tatcgagcca atatccaagg cgcattgaat gccggattaa
     1621 gagtcggtgt ctatattttc tcacaggcaa ccacacaggc ggaggcagcg gaagaggcaa
     1681 attatctttt aaatagaatc tctggctaca atatcacact gccggtggtc atagattatg
     1741 aattcggcac aaaccatagt ggacgtcttg cggatgccaa tctggatatt gatacggcaa
     1801 cggcagttgt gaatgctttt tgtaccacgg tgcagtcggc aggatatacg ccgatggtct
     1861 atgcgaataa gaccatgctg cagtcttaca taagaggtga aattctggac gactactata
     1921 agatctggct tgccaattat acgacgcaga cgacgtatgc aggcgagtat tacgcatggc
     1981 agtacagcag caagggcggc gtgagcggta taagcggtta cgtggattgc aa
//
LOCUS       DBMDQJ010000454         2026 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_18990_length_2026_cov_1.453506, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000454 DBMDQJ010000000
VERSION     DBMDQJ010000454.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18990_length_2026_cov_1.453506"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            30..>2026
                     /locus_tag="ACZO9R_09070"
     CDS             30..>2026
                     /locus_tag="ACZO9R_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913249.1"
                     /translation="MNGLAIEEQNRSSGMDTKVVYYTLPGENVGALVRVVSVTNQSGE
                     PIELELIDGMPAVIPYGVSMDSMKNMGQTAKAWMQVEDLSEGLPYYRVRASMDDTAAV
                     RRIDGGNFSACCEADGRRLQPIVDPSLIFSYDLSLKRPVGFEERPLKELLLEEQMTQN
                     LLPCSFYGITRTLAPGGSVTLYELIGQVENKQLLKEYFAEKKDAAYFEAKKREADELA
                     EALTDGIRTRTASAAFDAYCRYTYMDNVLRGGYPMQLGNNKIFYVYSRKHGDLERDYN
                     YFSMLPEFYSQGNGNFRDVNQNRRCDTFFAPFVGRKNIQEFYSLIQLDGYNPLGVEKL
                     TYRLSKERAKKLLADVKEEQRRALLDFATKPFTPGALCRKFGEVFGDTWDETLFIRVI
                     DFAEEMVNGSFGEGYWSDHWTYNLDLILDYLSVFPEQEEEMLYEEVYTTFLSRINVNR
                     RFRRYVETENGLRQYRALNEASRRADLGEKLVRTEYGSGDVLTMTLMEKLILLSAVKF
                     ATLDAYGMGIEMEGGKPGWYDALNGMPGLFGSSMAETYELARMLSYTIEALKQYPGEV
                     ALIEELGCFLDELNLITRLEHDNIMRDEELLSFWNRINDAKEIYRDKTYQGVSGKKMV
                     YHTEQLAAILEGFLEIVTCGIKKARRISGEICPTYFTYEVP"
BASE COUNT          509 a          470 c          618 g          429 t
ORIGIN      
        1 agaacatcgc gcaccggatg cagattcaca tgaatggtct tgcaatcgag gagcagaacc
       61 ggtcaagcgg aatggacaca aaagtggtgt actatacact gcccggagaa aatgtcggcg
      121 cgcttgtgcg cgtggtgagt gtcaccaatc agtccgggga gcccatcgag ctggaactga
      181 ttgacggcat gccggcggtg attccgtacg gcgtgtccat ggacagcatg aaaaatatgg
      241 ggcagaccgc gaaagcgtgg atgcaggtcg aggatttaag cgagggtctg ccgtactacc
      301 gcgtgcgggc aagcatggat gacaccgcgg cggtgcgcag gatcgacggc ggcaacttct
      361 cggcatgctg cgaagcggac ggcagacggt tacaaccgat cgtggatccg tctttgattt
      421 tttcctatga tctgtcgctg aaaagaccgg tcggttttga agaacgcccg ttaaaggaac
      481 ttcttctgga agaacagatg acgcagaacc tgctgccgtg cagcttttac ggcatcacgc
      541 gcacccttgc cccgggcgga agcgtcacct tatatgaact gatcggacag gtggagaaca
      601 agcagctttt aaaggaatat tttgcggaaa agaaagatgc cgcctatttt gaggcaaaaa
      661 aaagagaagc tgatgaactt gcagaggcgc tgacagacgg tatccgcacc aggacagcct
      721 ccgcggcgtt cgatgcctac tgcaggtaca cctatatgga caatgtgctg cgcggcggtt
      781 atccgatgca gctggggaac aataagatct tttatgtcta ttccagaaaa cacggtgatc
      841 tggagcggga ttacaattac ttttccatgc tgccggaatt ctattcgcag ggaaacggga
      901 acttccgcga tgtcaatcag aaccgccgct gcgacacctt ttttgcaccg ttcgtcggca
      961 gaaagaatat acaggaattc tacagtctga tccagctgga cgggtacaat ccgcttggcg
     1021 tcgagaaact gacctaccgc ctttcaaagg agcgggcaaa gaagctgctg gcagacgtga
     1081 aggaggagca aagaagagcg ctgcttgact tcgcgacaaa gccgtttacg cctggagcac
     1141 tctgccggaa gttcggggag gtattcggcg atacctggga tgagacgtta tttatccggg
     1201 tcattgattt tgcggaggag atggtgaacg gatcgttcgg ggaaggctac tggagcgatc
     1261 actggaccta caatctcgat ctgattctgg attacctttc cgtgttcccg gagcaggagg
     1321 aggaaatgct ctatgaggag gtctatacca cctttttgtc cagaatcaat gtgaaccgca
     1381 ggttccgccg ctatgtggag acggagaatg gcttaaggca gtaccgggcg ttaaacgagg
     1441 cgtccaggcg ggcggacttg ggggaaaaac tggtgagaac cgaatacggc agcggggatg
     1501 tgctgaccat gacgctgatg gagaaattga tactgctcag tgctgtgaaa tttgccacgc
     1561 tggatgctta cggaatggga atcgagatgg agggcggaaa gcccggatgg tatgacgccc
     1621 taaacggtat gccgggactt ttcggttctt ccatggcgga gacctacgag ctggcgcgca
     1681 tgctctccta caccatagag gccttaaagc aatatccggg agaagtcgca ctcatagagg
     1741 aactgggatg tttcctggat gaactgaatc tgatcacgcg gctggagcac gacaatatca
     1801 tgcgggacga ggaactgttg tccttctgga accggatcaa tgacgcgaag gagatttacc
     1861 gcgacaagac ctatcagggc gtatccggta agaagatggt ctatcacacg gagcagcttg
     1921 cggcgattct ggaaggattc ttggagattg ttacctgcgg aatcaaaaag gcgcgccgga
     1981 tctccgggga gatctgcccg acgtatttta cttacgaggt gccgga
//
LOCUS       DBMDQJ010000455         2021 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19044_length_2021_cov_1.500000, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000455 DBMDQJ010000000
VERSION     DBMDQJ010000455.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19044_length_2021_cov_1.500000"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            185..658
                     /locus_tag="ACZO9R_09075"
     CDS             185..658
                     /locus_tag="ACZO9R_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR0913250.1"
                     /translation="MKDREELLKEIALMLERQDMLSKLTENQCLDEYGYSETHCIDYI
                     GRLELPNVTKVAEHMGMTRGAISKMTKKLLAKGLIEKYTLETNKKEVYFKLTDQGRLL
                     FDEHAKRHQRWEKRDMEFLARYSVEDVRTVSRFMTEFNGYLEEQIETITGTQAEG"
     gene            complement(750..1451)
                     /locus_tag="ACZO9R_09080"
     CDS             complement(750..1451)
                     /locus_tag="ACZO9R_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013618.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR0913251.1"
                     /translation="MSITILNYHDQAFVDQINAEAAARAEAERAAAEAAKAQAASSDF
                     SSVLTNATDAYSGSTTCSGDLSAIFDEAANTYGVSSTLLKSIARAESNFNTSAVSSAG
                     AVGIMQLMPATAASLGVTDSYDAYQNIMGGANLISQLLARYNGDTSLALAAYNAGSAN
                     VDKYGGIPPFSETQNYVQKVLSYMGSDTNTVASASTAETSEDVATTVSNFIAANNISQ
                     DTVEKLLTLLEVLNA"
     gene            1534..>2021
                     /locus_tag="ACZO9R_09085"
     CDS             1534..>2021
                     /locus_tag="ACZO9R_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR0913252.1"
                     /translation="MKTKQVERKGTYLRAFRAAFPCTVPVLTGYLFIGMAFGVMIQEK
                     GYNFLWAMLMSLVIYAGSGQYLAVNFFAPGVSLLNVIFMEFMVNIRHIFYGLSLLERF
                     SKMGKKRLYMIFSLTDETYSLFFVTKVPKDVKEEQFLFAIAVLDQSYWIIGSGIGALL
                     GNV"
BASE COUNT          540 a          423 c          569 g          489 t
ORIGIN      
        1 ggaatatgac ctggacgagc tggatgccag acggctggag tgggggctgt tccgcgacag
       61 gcgcccggag tgctacggca tcatcagcgg caggtaaaag aaatatattg ttgacatgga
      121 aacaataatg agatacagta ggaactggga acaaaatacc atgttagaaa aaggaagagt
      181 ctgcatgaaa gatcgggaag agctgttaaa agaaatcgcg ctcatgctgg agcggcagga
      241 tatgctttcc aaactgacgg aaaaccagtg tctggatgaa tacggctatt cggagacaca
      301 ctgtatcgac tatatcggaa gactggaact gccgaacgtc acgaaggtgg cggagcacat
      361 ggggatgacg cgcggcgcga tcagcaagat gaccaagaag cttcttgcca aagggctgat
      421 cgaaaaatat acgctggaga cgaacaaaaa ggaagtatat ttcaagctca cggatcaggg
      481 caggcttttg tttgatgagc atgcgaagcg gcatcagcga tgggagaagc gcgatatgga
      541 atttctggcg cgctattccg tcgaggatgt gaggacggtg agccgtttta tgacggagtt
      601 caacggctat ctggaagagc agatcgagac gattacaggg acgcaggcag aaggatgata
      661 ttgtgtaata ggaacgaagt ttcctgttac acaaaaaaga atcctgcagg caggattctc
      721 atgtgttact gttgtatgaa atgtaattgt taagcgttta acacttcaag aagcgtaagc
      781 agcttctcta ccgtatcctg gctgatgttg tttgccgcga taaaattgct taccgtcgtt
      841 gcaacatctt cggaagtctc tgcagtggat gcggatgcaa cggtgttcgt atcgcttccc
      901 atataggaga gaaccttctg gacataattc tgtgtctcgc taaacggtgg aataccgccg
      961 tatttatcga catttgcact gccggcgttg tatgccgcaa gcgcaagaga ggtatcgccg
     1021 ttgtacctgg caagcagctg ggagatcaga ttcgcaccgc ccatgatatt ctggtaggca
     1081 tcgtaggaat ctgtgacacc gagagatgct gcggtggcag gcatcagctg cataataccg
     1141 acagcaccgg cgctgctgac agcgcttgta ttaaaattgg actctgctct tgcgattgat
     1201 tttaaaagag tggaggagac gccgtaggtg ttcgccgctt catcaaagat ggcggaaagg
     1261 tctccggagc aggtcgtgct gccggagtag gcgtcggtgg cattcgttaa aacggaagaa
     1321 aagtcagaac ttgcagcctg cgcttttgca gcttcggcgg cagctctctc cgcttcagcg
     1381 cgggcagcag cttcggcgtt gatctgatcc acaaatgcct gatcgtgata atttaagatc
     1441 gtaatactca ttggtaactc ccttgaaaac aacttcactg aatgtataac tagaaaaagt
     1501 atagcataat ttgcgggatt aggaaagaaa aaaatgaaaa ccaaacaagt cgagcggaaa
     1561 ggaacatatt tacgtgcatt tcgggcagcg tttccctgca cggtaccggt gctcaccggc
     1621 tatctgttta tcgggatggc gttcggagtg atgatacagg aaaaaggtta caacttcttg
     1681 tgggcgatgc tcatgagtct ggtgatctat gcgggcagcg gacagtatct tgcagtcaac
     1741 tttttcgcgc cgggcgtcag cctcctcaat gtgattttca tggaatttat ggtgaatatc
     1801 cgccatattt tttatggact gtcactgctg gaacgatttt ctaagatggg gaaaaagcgg
     1861 ctttacatga tcttttccct gacggatgag acctactcgc ttttcttcgt cacaaaagtg
     1921 ccgaaagatg tgaaggagga gcagttcttg tttgcgattg ccgtgctcga ccagagttac
     1981 tggatcatcg gctccggcat cggcgccctg cttggaaatg t
//
LOCUS       DBMDQJ010000456         2020 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19054_length_2020_cov_1.500261, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000456 DBMDQJ010000000
VERSION     DBMDQJ010000456.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19054_length_2020_cov_1.500261"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            147..941
                     /locus_tag="ACZO9R_09090"
     CDS             147..941
                     /locus_tag="ACZO9R_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR0913253.1"
                     /translation="MKKIAFFDIDGTLTSEIDGSIPESAVYAIRAARANGNLMFINTG
                     RCFQNVEQRFRDVGFDGFVCGCGTDIYCDGAEILHVAQTHETVMKLLLAAREWDIDIL
                     FESRKEVAFDLARPLRHPDARRQYEAFEERGYEMPEHLENPNFFCDKFVLWFEHPEQL
                     AGFRTTSDRYFECIDRGGNFREFVPLGYSKATGLNYVLDYYRIDKKDAYAFGDSNNDL
                     PMLTALTNSVAMGNASPATLFDKVSYVTKCASEDGIRLALEHFGFI"
     gene            complement(938..>2020)
                     /locus_tag="ACZO9R_09095"
     CDS             complement(938..>2020)
                     /locus_tag="ACZO9R_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013703.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913254.1"
                     /translation="KGAGRGAGARLFSNAICFVWMIGILFTLAGVFIPDKIIALMGGD
                     AGIVALGTGYTRIFLLFTPFFMMNYVIMAFVRNDNQPSLAMAGTLAGSFSNILFDYIF
                     MFPLGMGLEGAALATAASPIISILICSAHFFGKKNTVRFRLHAPSIAQLFGACQLGVP
                     AFVGEISSGVITTVFNFLILGLVGNIGVAAYGVIANFALVAMAIFNGVSQGAQPIVSD
                     CYGKGDVKSVRKIRNLGLVTALGIAVVLVGAVYGFTDSLVSLFNSEHSAELAEYAHTG
                     MRLYFIGFLFAGINIVGAGFLSATERARESFVTSIMRGFVCIIGCSLLFAAVFGINGV
                     WLSFPASELLTAIVMAVVLVRDNRRL"
BASE COUNT          527 a          549 c          487 g          457 t
ORIGIN      
        1 acagctgaat gtaatgtgac ggttatgtga cggaaccgtt aaatatctgt gtttcgcggc
       61 aggcagaccg cacatccttt gacagattag gtaaaaagta ttacaatact cccgtttcta
      121 tagatctgtt acattggaga ttattcatga agaaaattgc tttttttgat atcgacggaa
      181 cactgacttc cgagattgac ggttcgattc cggaaagtgc cgtctatgct atacgggcgg
      241 cgcgggcaaa cgggaatctg atgttcatca ataccgggcg ctgttttcag aatgtggaac
      301 agcgtttccg tgatgtgggt tttgacggct ttgtctgcgg ctgcggcaca gatatctact
      361 gcgacggtgc cgaaatcctt catgtcgcac agacgcacga gaccgtaatg aagctgcttc
      421 tcgcagccag ggagtgggat atcgatatcc tgtttgaatc caggaaagag gttgcttttg
      481 atctggcaag accgctccgg cacccggatg ccagacggca gtatgaggcg tttgaagaac
      541 gcggctatga gatgccggag caccttgaga atcctaattt tttctgtgat aagtttgtcc
      601 tatggtttga gcaccccgag cagcttgccg gtttccgtac cacaagcgac cgctattttg
      661 agtgcattga ccggggcggc aatttccgcg aattcgttcc gctcggctac tccaaagcca
      721 ccggtcttaa ctatgtgctt gattattacc ggatagacaa aaaggacgcc tatgccttcg
      781 gcgacagcaa caacgacctt cccatgctca ctgctttgac aaacagtgtc gccatgggca
      841 acgcctctcc tgccacgctt tttgacaagg tgtcctatgt aacaaaatgt gccagcgaag
      901 acggcatccg ccttgcgctg gaacatttcg gttttatcta aagtctgcgg ttatcccgca
      961 cgagcaccac agccatcacg atggctgtga gaagctcgga tgccgggaac gacagccaca
     1021 cgccattgat gccaaatact gcggcaaaaa gtagcgagca gccgataatg cagacaaatc
     1081 ctctcatgat cgatgtcaca aacgattccc gcgcacgttc tgtcgcgctt aaaaatcccg
     1141 cccccacaat attgattccg gcaaacagga agccgatgaa gtaaagacgc attcccgtgt
     1201 gagcatactc tgccagttcc gcagaatgtt cactgttaaa caggcttacc agagaatccg
     1261 taaatccata gacggctccg acgagcacta ccgcgatgcc caaagccgtg acaagtccga
     1321 gatttcgtat tttccggaca gatttcacat ctccttttcc gtagcagtca ctcacgatcg
     1381 gctgcgctcc ctgtgacact ccattaaaga ttgccattgc cacaagcgca aagttcgcga
     1441 tcactccgta agccgccaca ccgatatttc ccacaagacc gagaatcagg aaattgaaca
     1501 cggtcgtgat gactccggaa gaaatctccc ccacaaatgc cgggacgcca agctgacacg
     1561 ccccgaataa ctgggcgatg gacggcgcgt gcagccggaa ccgcacagta ttcttttttc
     1621 caaagaaatg cgcgctgcag atcagaatgc tgatgatcgg tgatgccgcc gtagcgagcg
     1681 ccgctccctc taaccccatg ccgagcggaa acataaaaat atagtcaaac aggatgttgg
     1741 aaaaacttcc cgcgagggtt cctgccatcg ctaaggacgg ctggttatcg ttgcgcacaa
     1801 atgccatgat gacgtagttc atcatgaaaa acggcgtaaa caacagaaaa atcctcgtat
     1861 acccggttcc aagcgccaca attcccgcat cgccgcccat cagcgcgatg attttatccg
     1921 ggataaaaac gcccgcaagt gtaaacaaaa ttccaatcat ccacacaaag cagatggcat
     1981 tggaaaatag ccgtgcgccc gctcctcgcc ctgcgccctt
//
LOCUS       DBMDQJ010000457         2019 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19070_length_2019_cov_1.558916, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000457 DBMDQJ010000000
VERSION     DBMDQJ010000457.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19070_length_2019_cov_1.558916"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1605)
                     /locus_tag="ACZO9R_09100"
     CDS             complement(<1..1605)
                     /locus_tag="ACZO9R_09100"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HIR0913255.1"
                     /translation="MGAIHYYEKERVFKLDTPHTSYLIGIVDEEKFLGHIYYGAKITD
                     YDVRALMRTGEAPFVPSQNNRDRSSFLDAFPMEYPGHGVGDYRESAISVSDKNGNTAV
                     QPLYREHKIYAGKPGLPGLPATFGSEEECETLEIVLADEVLDLTVTLYYTAFYDVDVI
                     TRSVRVTNHAKEPVYLTKVFSTALDLDAEDYRMLTLHGSWARERQIEYRQIGYGKQSV
                     GSCRGESSHQEHPFVALVSANAGQEQGRVYGFHFVYSGNFLAQVEKNQFDSLRVVLGI
                     HPQDFKWELKQDETFTAPEVVMTYSDAGLGRMTRTLHDLYRKHLIRSPYKDRERPVLI
                     NNWEATYFDFDTEKLLAIAREAKKSGIEMLVMDDGWFGHRNDDNTSLGDWQVNEQKIT
                     GGLKRLVDQVNEIGLKFGIWFEPEMISPDSELYRTHPDWAIALPGRMPCRSRNQYVLD
                     LSRKEVRDYAYECVASILRSANIAYVKWDMNRQLTDLGSGVLPVEKQGELLHRYVLGV
                     YELQERLITEFPQLLLENCSGGGARFD"
     gene            complement(1621..>2019)
                     /locus_tag="ACZO9R_09105"
     CDS             complement(1621..>2019)
                     /locus_tag="ACZO9R_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR0913256.1"
                     /translation="TLWDAMLDMYEKYGYYREGLETKTLKGIDGAAQIQSMMSEYRTN
                     PPKELGGHQVLAVRDYKEDTRKDLSTGEVTKTGLPTSNVLYYELSDNAWCCVRPSGTE
                     PKIKYYFGVKGTSLADADAKLAALKEDLLK"
BASE COUNT          440 a          634 c          450 g          495 t
ORIGIN      
        1 gtcaaatctc gccccaccgc cggagcagtt ctcgagaagc agctgcggaa attcggtgat
       61 cagacgctcc tgtaattcat agacgcccag tacatagcga tgcagcaatt ctccctgctt
      121 ttccaccgga agcacaccgc ttcccagatc ggtcagctgc cggttcatat cccacttgac
      181 ataagcgatg ttcgcgctgc gcaaaatgga tgccacacac tcatacgcat aatcccgcac
      241 ttcttttctg gagagatcga gcacatactg atttctcgac cggcacggca ttctccccgg
      301 aagggcaatc gcccagtccg gatgcgtccg gtacagctcc gaatccggcg agatcatctc
      361 cggctcaaac cagatgccga actttaatcc gatctcattc acctgatcga ccagccgttt
      421 taatccaccc gtaatcttct gctcgtttac ctgccagtcg ccgagacttg tgttgtcatc
      481 gttgcggtgg ccgaaccatc cgtcgtccat gaccagcatc tcgattccgg atttcttcgc
      541 ctctctcgca atcgcaagca gcttctccgt gtcaaagtca aagtaggttg cctcccagtt
      601 gttgatgagc accgggcgct ccctgtcctt atacgggcta cggatcagat gcttccggta
      661 gagatcgtgc agcgttctcg tcatcctgcc aagcccggca tccgaataag tcatcacaac
      721 ctccggtgcc gtaaatgtct catcctgctt taattcccac ttaaaatcct gcggatgaat
      781 tcccagcacc acgcgcaggg aatcaaactg gttcttctcc acctgcgcca ggaaattgcc
      841 ggaatacaca aaatggaatc cgtacacgcg cccctgctcc tgtccggcgt ttgcggacac
      901 cagcgccacg aacgggtgct cctgatggga agattcaccg cggcaggagc caacagactg
      961 ttttccgtag ccgatctggc ggtattcgat ctgtcgctct ctcgcccagg aaccatgcag
     1021 cgtcagcatc cggtaatcct ccgcgtcaag atccaacgcc gtagaaaata ccttcgtaag
     1081 atacaccggt tctttggcat ggttcgtcac gcgcacgctc cgcgtaatga catccacgtc
     1141 ataaaatgcc gtgtagtaca atgtgaccgt aagatccagc acttcatccg ccagcacaat
     1201 ctcaagcgtc tcacactctt cttcgctgcc gaacgtggcc ggaagtcccg gaagtcccgg
     1261 tttgcccgca tagatcttat gctccctgta aagcggctgc accgccgtat tgccgttttt
     1321 gtccgatacg gaaatcgcac tctccctgta atcgccgacg ccgtgccccg gatactccat
     1381 cggaaacgca tccagaaaag agctccggtc gcggttgttc tgtgaaggaa caaacggtgc
     1441 ctccccggtg cgcatcagcg cacggacgtc atagtccgtg atctttgcgc cgtaatagat
     1501 atgtcccaaa aacttttctt catccacgat accgatcaga taactggtat gtggtgtatc
     1561 cagtttaaaa acccgttctt tttcgtaata atgaattgct cccataccat ctcccgcttc
     1621 ttatttcaac agatcttcct tcaatgccgc aagctttgca tccgcatccg caagggaagt
     1681 cccctttacg ccaaagtagt atttgatctt cggctctgtt ccggacgggc gcacacagca
     1741 ccatgcattg tcggaaagtt cataatagag cacgttcgat gtcggcagtc cggtctttgt
     1801 cacctcaccg gtagaaagat cctttctggt gtcctccttg tagtcgcgca ccgccagtac
     1861 ctgatgaccg cccagttcct tcggcggatt cgtgcggtac tcactcatca tcgactggat
     1921 ctgtgccgca ccgtcgattc cctttaaggt cttcgtctcc aatccctctc tgtaatagcc
     1981 gtacttctca tacatatcca gcattgcatc ccacagggt
//
LOCUS       DBMDQJ010000458         2017 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19095_length_2017_cov_1.356994, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000458 DBMDQJ010000000
VERSION     DBMDQJ010000458.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19095_length_2017_cov_1.356994"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            80..1090
                     /locus_tag="ACZO9R_09110"
     CDS             80..1090
                     /locus_tag="ACZO9R_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HIR0913257.1"
                     /translation="MAITKIADVIVPELFNRYVINRTMELSAFFQSGIVVNSPKFDAL
                     ASEAARTHNMPFFEDLQGESEAILEDVKMTAKKIGSNKDVSTTIYRQNMWGASNLSAA
                     LAGADPMKAIGDLVASYWARDMQKELISILAGVFGTTTAGAEGTPAAETRMADHILDL
                     TTGKTDAAKQISASAFIDACQLLGDAQAQLSGVAMHSATKSYLKKLNLIETERDSTDV
                     EFDTYQGRRVTVDDGCPVTSGGVYTTYLFGNGAVAYGNGSPVGFVSTEVDRDKQTGGG
                     IDYLINRKAFILHPRGIAYTGAVREHVETPLRAELAKAENWKPVYEPKQLRIVAIKHK
                     IG"
     assembly_gap    1225..1234
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1235..1456
                     /locus_tag="ACZO9R_09115"
     CDS             <1235..1456
                     /locus_tag="ACZO9R_09115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HIR0913258.1"
                     /translation="IDEIPAGLETTVLRMAMDIYRNEHMGSANIPQTVSSVQIGDTTT
                     SFKTSAAEFSESLMKNYKPVLNRYRKVVF"
     gene            1456..1809
                     /locus_tag="ACZO9R_09120"
     CDS             1456..1809
                     /locus_tag="ACZO9R_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913259.1"
                     /translation="MDMVRMAIEAMYEDTCTVVEHRKTKEKGVVTYTDTVVLENQPCK
                     LSFETIAQAEKTDAASPVAQAVKLFVAPEVEIKSGSKIIVTHCGNSTEYTRSGVPGMH
                     PTHQEIMLDLFKEWA"
     gene            1809..>2017
                     /locus_tag="ACZO9R_09125"
     CDS             1809..>2017
                     /locus_tag="ACZO9R_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913260.1"
                     /translation="MGNTKVDLKQLEEFRDRVQKAADEEQQRAFMEACAKELAARLLA
                     KVIKRTPVGDYSGEAYECKGKDMNFT"
BASE COUNT          570 a          421 c          592 g          424 t
ORIGIN      
        1 taaaagaaaa tccggcgcag gcaaaagaac ttgcggcagc agccggagta acactgtaag
       61 aaagagagga tgataattta tggcaattac aaagattgca gacgtaattg taccggagct
      121 ttttaaccgg tatgtaatca acagaacaat ggagctgtcc gcgttcttcc agagcggaat
      181 cgtggtaaac agcccgaaat ttgatgcact ggcaagcgag gcagccagaa cacacaatat
      241 gccgttcttc gaggatttac agggtgagtc cgaggcaatc cttgaggatg tgaagatgac
      301 cgcaaagaaa atcggttcca acaaggacgt atccaccacg atttacagac agaatatgtg
      361 gggagcaagt aatctttctg cagcactggc cggagccgat ccgatgaaag ccatcggtga
      421 cctggtagca tcctactggg caagagatat gcagaaggag cttatctcaa ttcttgccgg
      481 agtgttcggc accactactg cgggggcaga gggaacaccg gcggcggaaa ccagaatggc
      541 agatcatatt cttgacctta ccaccggaaa aacggatgcg gcaaagcaga tcagcgcatc
      601 tgcgtttatt gacgcatgcc agctgctcgg cgacgcgcag gcgcagttat ccggcgtagc
      661 gatgcactcg gcgacaaagt cttatctgaa aaagctgaat ctgatcgaga cagagcgtga
      721 ttctacggat gttgagtttg acacctatca gggtagacgc gtgaccgtgg atgatggctg
      781 cccggtaact tccggtggcg tatatacgac atacctgttt ggcaatggtg cggtagctta
      841 cggcaatggt tctccggtag gatttgtttc aacagaggtt gaccgtgata agcagaccgg
      901 cggcggtatc gattatctta tcaaccgtaa ggcgtttatc ctgcatccga gaggaattgc
      961 atacaccgga gctgttcgtg agcatgtaga gacaccgctt cgtgcagaac ttgccaaggc
     1021 agagaactgg aaaccggtat atgaaccgaa gcagcttaga attgtagcaa tcaagcataa
     1081 gatcggttag gaggcgcggt atggcagagg aaagcaagct gacagtcgaa aggctgtcgg
     1141 cgcttctcgg gataagcgac ccggatgaga cagtgaaagt ccacttggag tttgcgcttg
     1201 aaaatgcaga ggatacggta aaaannnnnn nnnnatcgac gagattccgg caggactgga
     1261 aaccacggta ctgcgcatgg cgatggatat ttaccggaat gagcatatgg ggagtgccaa
     1321 tataccacag acggtttctt cggtgcagat cggcgataca acaacatctt ttaagacttc
     1381 cgcggcggag ttctcggaaa gccttatgaa gaattacaag ccggtgctga accgttaccg
     1441 gaaggtggtg ttttgatgga tatggtcaga atggcaattg aagccatgta tgaagatacc
     1501 tgcacggttg tggaacaccg taagaccaag gaaaagggcg ttgtgacata cacggacacc
     1561 gtggtcttgg aaaatcagcc gtgcaagctg tcgtttgaga cgatcgcaca ggcagaaaag
     1621 accgatgcgg catctccggt agcgcaggcg gtaaagctgt ttgttgcgcc ggaggtggaa
     1681 atcaagagcg gctccaagat catcgtgaca cactgtggca attccacgga gtacaccagg
     1741 agcggcgttc cggggatgca cccgacgcat caggaaatta tgctggattt atttaaggag
     1801 tgggcttgat gggaaataca aaggttgatc tgaaacaatt ggaggagttc cgggatagag
     1861 ttcagaaagc tgccgatgag gaacagcaga gagcgttcat ggaagcctgt gccaaggagt
     1921 tggcagcacg attgctggca aaggttatta agcgtactcc ggtaggggat tattctggag
     1981 aagcatatga gtgtaaagga aaggatatga actttac
//
LOCUS       DBMDQJ010000459         2015 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19113_length_2015_cov_1.047022, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000459 DBMDQJ010000000
VERSION     DBMDQJ010000459.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19113_length_2015_cov_1.047022"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            95..1195
                     /locus_tag="ACZO9R_09130"
     CDS             95..1195
                     /locus_tag="ACZO9R_09130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR0913261.1"
                     /translation="MKILIDARTLGKRPSGVGMYLYNFICGLMQYDTMKMELLTDVVE
                     SAAMKELDEAHIPIHRYGTPVEKSIGVYAYFRFVQNKINEVKPDLFWEVNNLTPVVIR
                     NPYGKTAVTVHDVFPITAPYGYGRLYPYYFKWNLGKTLRHMDAVIYNSLDTKTQVEQY
                     YPAAKALPSCISYIITEERPSLPVCDEGYFLYIGNLEERKGTDLLLKAYVRYRKDGGT
                     KKLYLAGKIRSDAIETLLGECQKETDGVCYQGYLETEEKYRMYAACSAFLFPSRAEGF
                     GMPVIEALTYRKPVLVSDLPIFHEIAGDAVCYCKLDTEDAVPQLAAAMRQIGEAGKES
                     VPDSLCEAVVGRYKKERLAGGLKEFLETIGKK"
     gene            1197..>2015
                     /locus_tag="ACZO9R_09135"
     CDS             1197..>2015
                     /locus_tag="ACZO9R_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913262.1"
                     /translation="MKQKMVRFIADHKQKLKKLLPKKLLRAAKLAYLKRLMAKYQKEP
                     IHPAEYQKKEAGVNLIGDIRAEIGLGQSMRLLANELELSKIPFGIYNFQLDGNVRRGD
                     HSFEHRMREDYPYRVNLFHVNPQEVGLAYLYLNKDIWRDRYNIAFWLWELEEFPQEYQ
                     EAIKFFDEIWTPSEFASSSIRKVTDKPVYTLPYYVTAGKDENCGRADFGLPEDKFLYL
                     IMYDTNSTMARKNPVGALDAYKKAFPVEDEHVGLVIKMNNPKQEDLEVLREQTKG"
BASE COUNT          542 a          423 c          619 g          431 t
ORIGIN      
        1 gctatatggg gattcccgta tggaaggcag tctactattt ctgctgttat gcggcagccg
       61 gatgtaagaa atatgcagga gcaagaggga aaaagtgaag attctgatag atgcgagaac
      121 gctcgggaag cgcccgagcg gcgtgggaat gtacctgtac aattttatct gtggcttaat
      181 gcagtatgat accatgaaaa tggaattact tacggatgtg gttgaaagtg cggcgatgaa
      241 agagctggat gaggcacata ttccgataca tcgatatggg acacctgtcg aaaaaagtat
      301 cggggtatat gcctattttc gttttgtgca gaacaaaata aatgaggtaa aaccggatct
      361 gttctgggaa gtaaacaatc tgacgccggt ggtcatccgc aacccgtacg gaaagacggc
      421 agtgaccgtg catgatgttt ttccgatcac ggcaccgtac ggatacggga gactttatcc
      481 atactatttc aagtggaatc tggggaaaac gcttcgccac atggacgcgg tgatctacaa
      541 ttccctggat acgaagacgc aggtggaaca gtattatccg gcggcaaagg cgcttccgtc
      601 ctgtatttct tatatcatta ccgaggagag gccttctctg ccggtctgcg acgagggata
      661 ttttctgtat atcggcaatc tggaagagcg gaagggaacc gatcttctgc tgaaagcgta
      721 tgtgcgctac cggaaggacg gcggcacaaa gaagctgtac cttgcaggaa agatccggtc
      781 ggatgcgatc gagacgctac tcggggagtg ccagaaggag acggacggcg tctgttacca
      841 ggggtatctg gagacggagg aaaagtaccg gatgtatgcc gcatgcagcg cctttttgtt
      901 cccgagcagg gcggaaggct tcggtatgcc ggtgatcgag gcgctgacct accgcaagcc
      961 ggttctggtg agtgatcttc cgatctttca tgagattgcg ggcgatgcgg tgtgctactg
     1021 taagctggac acagaggatg cggtgccgca gcttgccgct gcgatgcggc agatcgggga
     1081 agccggaaaa gagagcgtac cggatagcct gtgtgaagcg gtcgtcggac gctacaaaaa
     1141 ggagcgcctg gcgggcggat taaaagaatt cttggagacg atagggaaaa aatagcatga
     1201 aacaaaaaat ggtgcgtttt atcgcagatc ataagcagaa attaaagaag ttgcttccga
     1261 agaagctgtt gcgggcggca aagcttgcct acttaaagag gctgatggca aagtaccaga
     1321 aagagccgat tcatccggcg gagtaccaga aaaaagaagc gggcgtgaat ctgattggcg
     1381 acatccgggc ggagatcggt ctggggcaga gcatgcgtct gctggcgaat gaactggaat
     1441 tatcaaagat cccgtttgga atttataatt ttcagcttga cggcaatgtc cggcgcgggg
     1501 atcacagctt tgaacaccgg atgcgtgagg actatcctta ccgggtgaat ctgttccatg
     1561 tgaatccgca ggaggtagga ctggcgtatc tgtatctgaa taaggacatc tggagagacc
     1621 gttacaacat tgcattctgg ctgtgggagc tggaggaatt cccgcaggaa tatcaggagg
     1681 caatcaaatt tttcgacgag atctggacgc cctcggaatt tgcaagcagc agtatccgca
     1741 aggtgacgga caagcccgtg tacacgctgc cgtattacgt gacggcagga aaagacgaaa
     1801 actgcggcag ggcggacttt ggacttccgg aggacaaatt cttatatctg atcatgtacg
     1861 ataccaacag caccatggcg cgcaagaacc cagtgggagc gctcgatgcc tataagaaag
     1921 cgttcccggt ggaggatgaa catgtcggac tggtgatcaa gatgaacaat ccgaagcagg
     1981 aagatctgga ggtgctgcgc gagcagacga agggc
//
LOCUS       DBMDQJ010000460         2014 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19121_length_2014_cov_1.566649, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000460 DBMDQJ010000000
VERSION     DBMDQJ010000460.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19121_length_2014_cov_1.566649"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(285..357)
                     /locus_tag="ACZO9R_09140"
     tRNA            complement(285..357)
                     /locus_tag="ACZO9R_09140"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Thr,seq:ggt)
     gene            576..1283
                     /locus_tag="ACZO9R_09145"
     CDS             576..1283
                     /locus_tag="ACZO9R_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006771096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR0913263.1"
                     /translation="MEQYNILIVEDDKEIRDGIEIFLKSQNYNVFKAADGVEGLEVME
                     REKISLAIVDIMMPRMDGVTMTMKLREKYDFPVIMLSAKSEETDKVIGLNIGADDYVT
                     KPFTPLELMARVNSQLRRYTQFANRSPKEEKNDRIHVIGGLELNEDTVEVFVDGKPVK
                     MTPIEFKILALLIRTPGRVYSADEIYERVWNEKAINTDTIMVHVRNIRDKIEINPRDP
                     KYLKVVWGVGYKIEKQQ"
     gene            1267..>2014
                     /locus_tag="ACZO9R_09150"
     CDS             1267..>2014
                     /locus_tag="ACZO9R_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913264.1"
                     /translation="MKNNNRNNGAGNNNVSANNDASSNSGASRNNGASSNNSNAVNSS
                     KRIIIAIAGIIFITIITMCFFPVVYRTAERQKEQQKAEMMRESSDSVDNDTVQTLYQG
                     SYVLYVEQMERSGQADAGGLFLNLPTDDEDYQYVKDTVTSTFDEWKSQFESDSWNIDY
                     CVYKSSADCVKNTDQPLETCVDGTVTEKIKQYYTDIVCLSFDKEGALEVTPLYDENIS
                     GSVLIKAFQNVDRENLLGQSLGGYVEAGQLQ"
BASE COUNT          610 a          408 c          565 g          431 t
ORIGIN      
        1 ggaaaaggcg aagttccaga ggagaaaaat cgcgtgccag acccgtagag aatggaaaaa
       61 ggttgacata atgccagaag ggaagagcac cccgggtctg agacgagaaa aagttttcct
      121 actttcccct tggccacgag atagcattaa cgcctcaacc gtatattccc ccctcatcca
      181 gacttccatc acaatacatt cgtcataata ccgtaacact gtaacaccat agcacaaaaa
      241 agcgcaaacc ctttcattca aggatttgcg cttcttctca atgaagctgc tgacgggaat
      301 cggacccgtg acctccgcac taccaatgcg acgctctacc gactgagcca cagcagcatc
      361 tgtgtgagtt gcctcagcac ttgtactact atacactacg aagtggcagt tgtcaagatg
      421 ataattctaa ataattcttt agaaattctt cgggtatttc tgcggggaat tttgcggcag
      481 atcaggtaaa atgtaatcaa tgcacgggga ggcgtgtgat tctgcggcgg atgtgttatg
      541 atagcagaaa agagaacaag agcaggagcg gaaaaatgga acaatataac attttaattg
      601 ttgaggatga taaagaaatc agggacggaa ttgaaatctt tttaaagagt cagaattata
      661 acgtgttcaa ggcggcagac ggcgtggagg gacttgaggt gatggagcgg gagaagatct
      721 cgctggcgat cgttgacatc atgatgccgc gcatggacgg cgtcacaatg accatgaagc
      781 tgcgggaaaa atatgacttt ccggtcatta tgctctcggc aaagtcagag gagaccgaca
      841 aggtgatcgg gttaaatatc ggggcggatg attatgtgac caagccgttt acaccgttgg
      901 aactgatggc gcgtgtcaat tcccagctcc gccggtatac gcagtttgca aacaggagtc
      961 cgaaggagga aaagaacgac cggattcatg tgatcggagg actggaactg aatgaggaca
     1021 ccgtggaggt gtttgtggat ggaaaaccgg tgaagatgac gccgattgaa ttcaaaatac
     1081 ttgcgctttt aatccggacg ccggggcgtg tatattcggc ggacgagatc tatgagcgcg
     1141 tgtggaatga aaaggcgatc aataccgata cgattatggt gcatgtgaga aatatccggg
     1201 acaagattga gatcaatccg agagatccaa aatacttaaa ggtggtgtgg ggtgttggat
     1261 ataaaattga aaaacaacaa tagaaacaac ggtgccggca ataacaatgt cagcgcaaat
     1321 aacgatgcca gcagcaacag tggtgccagc agaaataacg gtgccagcag caacaacagc
     1381 aatgccgtaa acagcagcaa gcggattatc atagcgatcg cgggaatcat tttcatcacg
     1441 atcattacca tgtgtttttt cccggtggtg taccggacgg ctgagagaca gaaagagcag
     1501 cagaaagcgg agatgatgcg cgagtcctcg gacagcgtgg acaacgatac ggtacagaca
     1561 ctctatcagg gcagctatgt cctgtatgta gagcagatgg agcggagcgg gcaggcagat
     1621 gcaggcggac tgtttttgaa tctgccgacg gatgatgaag attaccagta tgtaaaggac
     1681 acggtaacct ctacgtttga cgaatggaaa agccagtttg agagtgacag ctggaacatc
     1741 gattactgtg tatacaaaag cagtgccgac tgcgtgaaaa atacggatca gccgctcgag
     1801 acatgcgtgg atggaactgt gacggagaag ataaagcagt attatacgga catcgtatgc
     1861 ttatcctttg acaaagaggg cgcgctggaa gtgacaccgc tgtacgacga aaatatcagc
     1921 ggcagcgttc tgatcaaggc gtttcagaat gtagaccggg aaaatctttt gggacagtcg
     1981 ctgggcggat atgtggaagc gggtcagttg cagg
//
LOCUS       DBMDQJ010000461         2013 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19139_length_2013_cov_1.415272, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000461 DBMDQJ010000000
VERSION     DBMDQJ010000461.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19139_length_2013_cov_1.415272"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="ACZO9R_09155"
     CDS             complement(<1..760)
                     /locus_tag="ACZO9R_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR0913265.1"
                     /translation="MRITMKQEEQIIRTEYSELMQKSYIDYAMSVIISRALPDIRDGL
                     KPVQRRTLYDMYELGIRYDKPYRKSARIVGDTMGKYHPHGDSSIYGALVVLAQDFKMG
                     QTLVDGHGNFGSIEGDGAAAMRYTEARLQKLTQEAFLADLDKDVVDFVPNFDETEKEP
                     EVLPVKVPNLLINGAEGIAVGMTTSIPTHNFGEVIDGVIAYMKNPDINTEQMMQYIPG
                     PDFPTGGVIANKDELTAIYSTGVGKIKIRGKVEVE"
     gene            complement(827..>2013)
                     /locus_tag="ACZO9R_09160"
     CDS             complement(827..>2013)
                     /locus_tag="ACZO9R_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR0913266.1"
                     /translation="YFKGETEGITVEAAFQYTNEFHENILGFCNNIFNAEGGTHITGF
                     KTVFTTVINAYARELGILKDKDANFTGADVRNGMTAVVSIKHPDPRFEGQTKTKLDNQ
                     DASRATAKVTGDEIQLYFDKNLEVLKTVISCAEKAAKIRKTEEKAKTNLLTKQKFSFD
                     SNGKLANCESRDASICEIFIVEGDSAGGSAKTARDRSFQAILPIRGKILNVEKASIDK
                     ILANAEIKTMINAFGCGFSEGYGNDFDITKLRYDKIIIMADADVDGAHISTLLLTLFY
                     RFMPELIYEGHVYVAMPPLYKAIPSRGEEEYLYDDEALERYRRSHKGSFTLQRYKGLG
                     EMDAEQLWETTLDPKRRLLKRIEIEDGRMASDITELLMGNDVPPRKTFIYEHATDATV
                     DA"
BASE COUNT          407 a          624 c          462 g          520 t
ORIGIN      
        1 gctcgacctc cacttttcca cggatcttga ttttccctac gcccgtagag tagatggctg
       61 tcagctcgtc cttgttggcg atcacaccgc cggtcgggaa atccggtccc gggatatact
      121 gcatcatctg ctcggtgttg atgtccggat tcttcatata ggcaatcacg ccgtcaatga
      181 cctcgccgaa attgtgcgtc ggaatgctcg tcgtcatgcc gaccgcaatt ccctccgcac
      241 cgttgatcag aagattcggc acctttaccg gcagaacctc cggctctttc tccgtctcgt
      301 caaagttcgg cacaaaatcc acgacatcct tgtcaaggtc tgctaagaac gcctcctgcg
      361 tcagcttctg aagtctcgcc tccgtgtaac gcatggcagc cgctccgtct ccctcgatgg
      421 agccgaagtt gccgtgtccg tccaccagcg tctgtcccat tttaaaatcc tgtgccagca
      481 ccaccaacgc accgtagatc gaactgtctc cgtgcgggtg atacttaccc atggtatccc
      541 cgacaatacg cgcactctta cggtacggct tgtcatagcg gatgccgagt tcatacatat
      601 cataaagtgt ccgcctctgt accggcttta acccgtccct gatatccggc agtgcacgtg
      661 agataatcac gctcatcgca tagtcgatat aggatttctg catcagctcc gaatactcgg
      721 tacggataat ctgttcttcc tgtttcatgg taatcctcat ttcctgccgc ttcttcctgc
      781 ggctctctca tcccgcgcgt gatcctactg cacggtatcg cgtctgtcat gcgtccacgg
      841 tcgcatccgt cgcatgctca tagataaagg tcttgcgcgg cggtacgtca ttgcccatca
      901 gaagctccgt gatgtcggac gccatgcggc cgtcctcaat ctcaatccgc tttaacagac
      961 gtctcttcgg atcgagcgtc gtctcccaga gctgctccgc atccatctcg ccgagacctt
     1021 tgtaccgctg cagcgtaaaa ctgcccttgt ggctccgtct atagcgctcc agcgcctcat
     1081 catcgtagag gtactcttcc tctccgcggc ttggaatcgc cttgtaaagc ggcggcattg
     1141 ccacatagac atgtccctca tagatcagtt ccggcatgaa ccggtagaat aacgtcaaaa
     1201 gcagcgtgga aatatgcgct ccgtcgacgt cggcatccgc catgatgata atcttgtcat
     1261 agcggagctt cgtgatgtcg aaatcattgc cgtagccctc ggaaaatccg cagccgaacg
     1321 cattgatcat cgtcttgatc tccgcgttgg ctaaaatctt atcgatgctc gccttctcca
     1381 cgtttaaaat cttaccgcgg atcggcagaa tcgcctgaaa gctgcggtct ctcgccgtct
     1441 ttgcggaacc tcccgcggaa tctccctcga cgataaaaat ctcgcagatc gacgcatccc
     1501 tgctctcgca gttcgcaagc ttgccgttgg agtcaaagga gaacttctgc tttgtcagaa
     1561 gatttgtctt cgccttctcc tcggtcttgc ggatcttcgc cgccttttcc gcacaggaaa
     1621 tgacggtctt aagcacttcc aggttcttgt caaaatagag ctggatctcg tcgccggtca
     1681 ccttcgccgt cgcacgcgac gcgtcctgat tgtccagctt cgtcttcgtc tgtccctcaa
     1741 aacgcgggtc cggatgcttg atggagacga ctgccgtcat gccgttgcgc acgtccgcgc
     1801 cggtaaaatt ggcgtcctta tccttcaaga tgccaagctc tctcgcatag gcattaataa
     1861 cagtcgtaaa caccgtctta aaaccggtga tgtgtgttcc gccctccgcg ttgaaaatat
     1921 tattacagaa gcccagaata ttctcatgaa attcgttcgt gtactggaac gccgcctcga
     1981 cggtaatgcc ctcagtctct cccttaaaat aca
//
LOCUS       DBMDQJ010000462         2004 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19244_length_2004_cov_1.511823, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000462 DBMDQJ010000000
VERSION     DBMDQJ010000462.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19244_length_2004_cov_1.511823"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1483
                     /locus_tag="ACZO9R_09165"
     CDS             <1..1483
                     /locus_tag="ACZO9R_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycogen operon protein GlgX"
                     /protein_id="HIR0913267.1"
                     /translation="PRHEMFLYKLNIRGFSMDAGIPAKERGTFAAVRDKIPYLKELGV
                     TTVEFMPVYEFEELILKEKAVLPEYISWQVRKEDQIKPEACAMPERVNLWGYAPANYF
                     AVKASYAKGQDAAVEWKKLIRALHENHMECVMEMYFPENVNQNLILDALRYWVREYHV
                     DGFHLLGENLPVTQIAQDAWLRRTKIFCAGFENAVLWHPCRYPHLYVYNDEYLYPVRA
                     LLNHKNGGMDAFACQQRKQHQYHGFVNYISDNNGFSLRDLFCYEQKHNEDNGEENRDG
                     SDWNLSANYGVEGRSRKQRIVRIRERQMCNAVAVLFLAQGVPLLYEGDETGDSQEGNN
                     NAYCQDNPTGWVNWKKDERYAWFREFVARMAAFRREHPVLAADMPKQLSDAKGTGYPD
                     LSYHGENAWISDLSGERQSLGMLYCGKEDGDGTEGEKFLYVGCNFHMGPTRLALPKLP
                     GKKKWYLLMDTARERAPFLKEEERVDAPRIELCGQSVVILTGK"
     gene            1496..>2004
                     /locus_tag="ACZO9R_09170"
     CDS             1496..>2004
                     /locus_tag="ACZO9R_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5662 family protein"
                     /protein_id="HIR0913268.1"
                     /translation="MKAWKHFCTINHHKHLVMKGCFAVGLYKQGLLHDLSKYTPTEFL
                     VGCKYYQGTMSPNNAERKDKGYSSAWLHHKGRNKHHMEYWIDYGVPDEGGDKGLCGMK
                     MPLRYVVEMYIDRVAASKNYQKERYTDRSALEYYLHGKDFHVMHKDTRALLELLLYRL
                     AVRGEQEVNT"
BASE COUNT          514 a          437 c          612 g          441 t
ORIGIN      
        1 cccgcgccat gagatgttcc tctataaact caacatccgc ggattttcga tggatgcggg
       61 aatccctgca aaggagcggg gaacttttgc ggcggtgcgc gataagattc cctatctgaa
      121 agaacttggt gtcacgacgg tggagttcat gccggtgtat gagtttgagg aactgatctt
      181 aaaggagaag gcggtattgc cggaatatat cagctggcag gtgcggaaag aggatcagat
      241 caagccggaa gcatgtgcga tgccggagcg tgtgaacctg tggggatatg cgcccgccaa
      301 ttattttgcg gtaaaggcgt cctacgcgaa agggcaggat gcggccgtcg aatggaaaaa
      361 actcatccgc gcgctccatg agaatcacat ggaatgtgtg atggagatgt attttcctga
      421 gaatgtcaat cagaatctga tcttagatgc gctgcgttac tgggtcaggg agtatcatgt
      481 ggatggcttc catctgctgg gggagaatct gcccgtcacg cagattgcac aggatgcgtg
      541 gcttagacgg acgaagattt tctgcgccgg tttcgagaac gctgtgctct ggcacccgtg
      601 ccgttatccg catctgtatg tatacaatga cgaatatctg tatcccgtcc gggcgctgct
      661 caatcacaag aacggcggta tggatgcatt tgcatgccag cagcgcaaac agcatcagta
      721 tcacggattt gtcaattaca tttcggacaa taacgggttt tccctgcggg atctcttctg
      781 ctatgagcag aaacacaatg aagacaacgg ggaggagaac cgggatggaa gcgactggaa
      841 tttgagcgcc aactacggcg tggagggacg aagccgcaag cagcggatcg taagaatccg
      901 tgaacggcag atgtgcaatg cggtggcggt gctttttctc gcgcagggag tcccgctttt
      961 atacgagggg gacgagacgg gagattccca ggaaggcaac aacaatgcat actgtcagga
     1021 caatccgacc ggctgggtga actggaagaa agatgagcgc tacgcgtggt tccgggagtt
     1081 tgtggcgcgg atggcagcgt ttcgcaggga gcatcccgtg ctcgccgcgg atatgccaaa
     1141 acagctttcc gatgcaaaag gaacggggta tcccgatttg tcctatcacg gagagaatgc
     1201 gtggatttca gatctttccg gcgagagaca gtctttgggc atgctctact gcggaaaaga
     1261 ggatggcgat ggaacagagg gggagaaatt cctttatgtc ggttgcaatt ttcacatggg
     1321 tcctaccaga ttggcgcttc cgaagcttcc cgggaagaaa aagtggtatc tgctgatgga
     1381 caccgccaga gagcgggcac cgttcctaaa agaggaggag cgggtggatg cgccgcggat
     1441 cgaactttgc ggtcagtctg tcgttatatt aacaggaaaa taaggttaca ttattatgaa
     1501 agcatggaaa catttttgca cgatcaacca tcacaagcat ctggtcatga aaggctgttt
     1561 tgcggtgggg ttatacaaac agggattatt acatgatctg tcgaaatata cgccgacaga
     1621 atttctggtg ggctgtaaat attatcaggg aacaatgagc ccgaacaatg cagagcgcaa
     1681 ggataaggga tactcgtccg cgtggctgca ccacaaaggg cggaacaagc atcacatgga
     1741 atactggatt gactacggcg tgccggacga gggcggggac aagggactct gcggcatgaa
     1801 aatgcctctg cggtacgtgg tggagatgta tatcgaccgt gtagcggcct cgaaaaatta
     1861 ccagaaggag cgctataccg accggagcgc attagagtat tacctgcatg gaaaggattt
     1921 tcatgtcatg cacaaggata cgcgggcgct tcttgaactg cttttgtacc ggctcgctgt
     1981 ccggggcgag caggaagtca atac
//
LOCUS       DBMDQJ010000463         2004 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19248_length_2004_cov_1.219128, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000463 DBMDQJ010000000
VERSION     DBMDQJ010000463.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19248_length_2004_cov_1.219128"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            365..1387
                     /locus_tag="ACZO9R_09175"
     CDS             365..1387
                     /locus_tag="ACZO9R_09175"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR0913269.1"
                     /translation="MDRKYDVITLGEILLRLSTPINGRLAQGDTLMKHLGGAELNIAS
                     EIGQLGLKTAIISKIPNNLLAAFIKNQLNASRVSENYLIEDHSDDSRVGIYYYEYGSY
                     PRKPRVVYDRKHSSINNIDIKDVPATMFYNTRLFHTSGITLGLGGNAQKFAIECIRKF
                     KENGTLISFDVNYRANLWSGEEARKCIESILPYVDIFFCSADTARLTFGKTGTIEEIQ
                     KSFCEEYPISVVASTERTVITPKKHDFTSIIYSAKEDRYYSESPYNSIDVVDRIGSGD
                     AYVSGALYGYLRHHDCQKMLEYGNAMAAIKHSVIGDMVFTDLEEIDGIIAQHKDTSGF
                     QPEMNR"
     gene            complement(1642..>2004)
                     /locus_tag="ACZO9R_09180"
     CDS             complement(1642..>2004)
                     /locus_tag="ACZO9R_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HIR0913270.1"
                     /translation="DPMQIYNSDEGIRKVVNQLVDGTFSPNDPGLFRDLYNSLLNTQC
                     TQYADTYFILKDFRSYVDAQKRIVEYYQDKQAWAKSAILNTAHAGKFSSDRTIQEYVD
                     EIWHLDHITVPDALVAKM"
BASE COUNT          503 a          535 c          442 g          524 t
ORIGIN      
        1 tgtaacgact ttctttgcca ttattgaatc ctcctaaatg tatgtttcac catgtgaaac
       61 cgtgtttcat tttctataat aaaattataa catctaatac cagaatgtca aatatgtttt
      121 tatttaagtc caaaaaaatt ccaaattatt tccgattttc acttttctat tttagcagga
      181 tgatatataa taaaaacaga ttggttcaca ttacagaact atgtttcgca atgtggaaca
      241 tacatatcgt atccatagac acagtgtgct gcattttctc ataaagcatt ctttttgaga
      301 tgccgtgacg tgtcggaatt tttactttct tatttattct tacatcatca ggaggagatt
      361 atttatggac cgtaaatacg atgtcatcac tttgggggag attttactcc ggctttccac
      421 gccgatcaac ggacgtcttg cacagggcga cacgctgatg aagcacctcg gcggtgccga
      481 gctcaacatt gcttccgaga tcggacagct cggccttaag acagcaatca tttcaaagat
      541 tccgaacaac ctgctggccg ctttcatcaa gaaccagctc aacgcttccc gcgtcagcga
      601 gaattatctg atcgaggatc actcggacga cagccgcgtc ggcatttact actatgagta
      661 cggctcctac ccgagaaagc cgcgtgtggt ctatgacaga aaacattcat ccatcaacaa
      721 tattgacatc aaggacgttc cggctaccat gttctacaat accaggctgt tccacaccag
      781 cggtatcact ttaggactcg gcggcaacgc acagaagttc gcgatcgaat gtatccgcaa
      841 gttcaaggag aacggcaccc tgatctcctt cgatgtcaac taccgcgcaa acctctggag
      901 cggcgaggag gcaagaaaat gcatcgaatc catccttccg tatgtcgaca tctttttctg
      961 ctccgccgac actgcccggc tgaccttcgg caagaccggc acgattgagg agatccagaa
     1021 gtccttctgt gaagaatatc cgatctccgt ggttgcctcg actgagcgca ctgtcatcac
     1081 tccgaagaag cacgacttta cctccatcat ctactctgcg aaggaagacc gctactacag
     1141 tgaatctcct tacaacagca tcgatgtggt cgaccggatc ggaagcggtg acgcctacgt
     1201 ctccggcgct ctctacggtt acctgagaca tcacgactgt cagaaaatgt tggagtacgg
     1261 caacgccatg gcagccatca agcacagcgt catcggcgat atggtcttta ccgatctcga
     1321 ggagatcgac ggcatcatcg cacagcacaa ggatacttcc ggattccagc ctgagatgaa
     1381 ccgctaagta tcattttcct gcgaaggaat gaagcgtgcg cctgccgcac actttgcgcg
     1441 gcagataaaa aaatgatggc ggcagtcccg cggatactct ccgccggact gccgccgttt
     1501 ttattctgaa tcttctctat ggttccgccg ccggctcctt tcatcagagc cggctctttt
     1561 ccggaatcac gatctttttc tcttctgtcg aaggagaacg ttcctcatcc gcctccggag
     1621 catttccggt gcggtgtctc ttcacatttt cgcaaccagt gcatccggca cggtgatgtg
     1681 atccagatgc cagatctcat ccacatactc ctgaattgtg cggtcggaag agaatttgcc
     1741 tgcgtgggcc gtatttaaaa tagcgctctt cgcccatgcc tgcttatcct gatagtactc
     1801 cacaatccgc ttctgcgcat ccacatagga acggaaatcc ttgaggataa aatacgtgtc
     1861 tgcatactgc gtgcactgcg tgttgagcag tgaattgtag agatcacgga acaaccccgg
     1921 atcgttcggc gaaaatgtgc cgtccacaag ctgatttacc accttgcgga taccctcgtc
     1981 gctgttgtag atctgcatcg gatc
//
LOCUS       DBMDQJ010000464         2001 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19283_length_2001_cov_1.182105, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000464 DBMDQJ010000000
VERSION     DBMDQJ010000464.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19283_length_2001_cov_1.182105"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /locus_tag="ACZO9R_09185"
     CDS             complement(<1..168)
                     /locus_tag="ACZO9R_09185"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242599.1"
                     /note="Converts isocitrate to alpha ketoglutarate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HIR0913271.1"
                     /translation="MEKIKMTTPLVEMDGDEMTRILWKMIKDELLYPFIDLKTEYYDL
                     GLEHRNETDDQV"
     gene            complement(225..887)
                     /locus_tag="ACZO9R_09190"
     CDS             complement(225..887)
                     /locus_tag="ACZO9R_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR0913272.1"
                     /translation="MSEDNYSLSSRVFHTIRENILSGKYATDEELKEKSIGEELGVSR
                     TPVREALRQLELEGLVTIIPNKGAYVVGISQKDIRDIYEIRSRLEGLCARWACDNITK
                     DQLDELEENIYLSDFHSSKGNSEQVVELDNKFHEILYDASGSKELRHILRDFHHYVQR
                     VRKVTLAVQERAVNSNEEHRKIVEALKQHDGARAEELADAHMRNTINNMDHYGWDNLL
                     KQ"
     gene            complement(902..>2001)
                     /locus_tag="ACZO9R_09195"
     CDS             complement(902..>2001)
                     /locus_tag="ACZO9R_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HIR0913273.1"
                     /translation="LVRQSEFFLYSKKDRDAVYKCLERGYKFPEVTSWIRASKQDFQL
                     VKDIGLRETGILVSCSDYHIFYKMKMTRREVMNLYLSVIRECLETGISPRCHLEDITR
                     SDIYGFVIPFCVELMKLMDEYKIPIKIRACDTMGYGVNFPGAVIPRSVPGIIYGLTTH
                     AGVPSELIEWHGHNDFYKAVNNSTTAWLYGASGVNCSLFGIGERTGNTPLEAMVFEYA
                     QLKGTLDGMDTTVITELAEYFEKELGYVQPSRTPFVGSNFNVTRAGIHADGLLKNEEI
                     YNIFDTGKFLNRPPLVSVSNTSGLAGIAHWINSYYHLPKEKWVDKNSDLVKKIKVWVD
                     AQYEDGRMTVLTDSELVEEIGKCCKELNITI"
BASE COUNT          432 a          579 c          425 g          565 t
ORIGIN      
        1 aacctgatcg tccgtctcgt tgcggtgttc cagtccgaga tcataatact ccgtcttaag
       61 atcgataaac ggatacaaca gttcgtcttt gatcattttc cagagaatcc gtgtcatctc
      121 atctccgtcc atctctacaa gtggcgttgt cattttgatt ttttccatgg ctgcttcctt
      181 tctgtgctgt gattctgttc tatctgacat ggcatcttcc gctcttactg cttgagcaga
      241 ttgtcccagc cgtaatgatc catattgtta atggtgttgc gcatatgtgc gtccgccagt
      301 tcttccgccc tggccccgtc atgctgcttt agcgcctcca caatcttgcg gtgctcctcg
      361 tttgaattga ccgctctctc ctgcacggcg agcgtcacct tgcgcacgcg ctgcacgtaa
      421 tggtgaaaat cccgcaggat gtggcgcagc tccttgctgc ctgacgcatc gtataaaatt
      481 tcatggaact tgttgtcaag ctccaccacc tgctcggaat ttcccttgga cgagtggaaa
      541 tcggacagat aaatgttctc ctccagctca tccagctgat cctttgtgat gttatcgcac
      601 gcccacctcg cgcagagtcc ctccagcctg gagcggatct cataaatatc gcggatatcc
      661 ttctgggaga ttccgacgac atatgctccc ttgttcggaa tgatggtcac aagaccctcc
      721 agttccagct ggcgcagtgc ctccctgacc ggagtccggc tgacacccag ttcctctccg
      781 atggattttt cttttaattc ctcgtccgtc gcatatttcc cggacagtat attttcacgt
      841 attgtgtgaa acacgcgcga actcagcgaa taattatcct cactcatcta tttacctcat
      901 gctatatcgt gatgttcagc tctttgcagc actttccgat ctcttccaca agctcggaat
      961 ccgtaagtac cgtcatacgt ccgtcctcat actgtgcatc cacccagact tttattttct
     1021 taaccagatc cgaattctta tcgacccatt tctccttcgg cagatgatag tacgaattga
     1081 tccagtgtgc aattccggca agacccgatg tgttggacac cgatacaaga ggcggtctgt
     1141 ttaagaattt ccccgtatca aaaatgttgt aaatctcctc gttcttgagc agtccatctg
     1201 catgaattcc cgctctcgtc acattgaagt tgcttccgac aaacggtgtg cggctcggct
     1261 gtacatagcc gagttctttt tcaaagtatt ctgccagttc tgtaatgacc gttgtatcca
     1321 taccgtcaag tgttcccttt aactgcgcat actcaaagac catcgcctca agcggtgtat
     1381 ttccggtgcg ctctccgata ccgaacaggg agcagttaac gccgcttgcg ccgtaaagcc
     1441 atgcggttgt ggagttattg accgccttgt aaaaatcgtt gtggccatgc cactcaatca
     1501 actccgacgg aacgccggca tgggtggtca gtccgtaaat aatacctggc acgctccgtg
     1561 ggatcacggc acccgggaaa ttcacgccgt atcccatggt atcgcacgcg cggatcttga
     1621 tcgggatctt gtactcatcc atcagcttca tcagttccac acagaacgga atgacaaacc
     1681 cgtaaatatc cgacctcgta atgtcctcca gatggcagcg cggactgatt cccgtctcca
     1741 ggcactcgcg gatcacgctc aggtacagat tcatgacctc acgtctggtc atcttcatct
     1801 tataaaaaat atgataatcc gagcagctta ccagaatacc ggtctctctg agtccgatgt
     1861 ccttcaccag ctggaaatcc tgcttgcttg cacggatcca gctcgtaacc tccgggaact
     1921 tgtatcctct ttccaggcac ttatagaccg catccctgtc cttcttgctg tacaggaaaa
     1981 attcactctg acggaccagt c
//
LOCUS       DBMDQJ010000465         1999 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19304_length_1999_cov_2.057956, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000465 DBMDQJ010000000
VERSION     DBMDQJ010000465.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19304_length_1999_cov_2.057956"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..928)
                     /locus_tag="ACZO9R_09200"
     CDS             complement(<1..928)
                     /locus_tag="ACZO9R_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR0913274.1"
                     /translation="MKKRAVTLLTLLAVSASLMACGGSDATESSAENDAEQIEAEVTG
                     QTENADTEEEEVLPEGKYRSELTNELIDESLKDQRPIAVMVDNESIALPHYGLSHADV
                     VYEMMNSTLNGRITRFMALFKDYESVDQIGSIRSVRPTNVILAAEWNAIICHDGGPFY
                     VDEYLDEACSDHFSGTFSRVDNGKSREYTEYILSGDMDKNFSNSDVSKDYTSYYEGAH
                     YQFANENNPVDLGTSYPDDAIDANMVDLPFPHNGSYLEYNADDQLYYYSEYGKAHVDP
                     GNDNAQLCFKNLLIQSCGYTQYDEHGYMIFDCI"
     gene            complement(1042..1986)
                     /locus_tag="ACZO9R_09205"
     CDS             complement(1042..1986)
                     /locus_tag="ACZO9R_09205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0913275.1"
                     /translation="MLISLIVPCYNEQEALPIFYEESTKVLAGMNCEYEFLFVNDGSK
                     DATLSILKGLAEKDEHVTYLSFSRNFGKEAAMYAGFTNARGDYVAVMDADMQDPPALL
                     PQMFAMLTSGEFDSVATRRVNREGEPPIRSWFARQFYRIINRISDADIVDGARDFRLM
                     KREMVDAIVEMGEYNRFSKGIFGWIGFRTYWLPYENVNRVAGETKWNFWKLLKYAIDG
                     IINFSQAPLAVASWFGMGMTLLSFIAVIFIVVRKLVFGDPVTGWASTACIITFIGGVQ
                     LFCMGIMGQYIAKTYMEVKQRPHYIVAESNRAEVTRIK"
BASE COUNT          499 a          563 c          397 g          540 t
ORIGIN      
        1 tgatgcagtc aaagatcata taaccgtgtt cgtcatactg tgtatagccg cagctctgaa
       61 tcagaagatt cttaaagcac agctgtgcgt tgtcattgcc cggatctaca tgggctttgc
      121 cgtactcgga ataatagtaa agctgatcat ccgcattgta ctccagataa gatccgttgt
      181 gcgggaatgg aagatctacc atattcgcat caatcgcatc atccggataa gatgtcccga
      241 gatcaaccgg gttgttctca ttcgcgaact gataatgtgc tccctcataa taggaagtat
      301 aatcctttga cacatcggag ttggagaagt tcttgtccat gtccccggaa agaatatact
      361 ctgtatattc tctggacttg ccgttatcca cacgcgaaaa tgttccgctg aaatgatcgg
      421 aacatgcctc gtccagatac tcatccacat agaacggacc tccgtcatga cagatgatgg
      481 cattccattc cgcagccagt atcacgttcg tcggtctgac gctgcggata cttccaatct
      541 gatccacact ctcataatcc ttaaacagcg ccataaatcg tgtaatgcgt ccgttcagcg
      601 tactgttcat catctcatat accacatctg catgtgacaa tccataatgc gggagtgcaa
      661 tggattcatt gtctaccata acagcaatcg ggcgctgatc cttcagactt tcatcaatca
      721 gctcgttcgt cagctcactg cggtattttc cttccggaag aacctcctct tcctcggtgt
      781 ccgcattctc cgtctgccct gttacctctg cctcaatctg ctctgcgtca ttctccgcgc
      841 tgctctccgt ggcatcgctt cctccgcatg ccataaggct ggcggataca gcaagcagtg
      901 tcaataatgt aactgctctc ttcttcacta atgtctacct ctcaatatct ttattttcta
      961 ccggaatata tctgcagtca gatctgcgga tatatctgat ttcattctac catatctcaa
     1021 gacgctgtaa aagccctttt tctacttaat ccgagtcact tcggcacggt tgctctccgc
     1081 aacaatataa tgcgggcgct gctttacctc catgtaagtc tttgcaatat actgccccat
     1141 aattcccatg cagaaaagct gcacgccgcc gatgaacgtg atgatgcacg ccgttgatgc
     1201 ccagccggta accggatctc cgaacaccag cttgcgcacc acgataaaaa tgaccgcgat
     1261 aaaggaaagc agcgtcatcc ccatgccgaa ccaggatgcc acggcaagcg gcgcctgtga
     1321 aaaattgata atgccgtcaa tcgcatattt taacagcttc cagaaattcc actttgtctc
     1381 tcctgccaca cggttcacgt tttcatatgg cagccagtat gtccggaagc caatccatcc
     1441 aaagattccc ttggaaaaac ggttgtattc tcccatctcc acgatcgcgt ccaccatctc
     1501 ccgcttcatc agcctgaaat cacgggcacc gtctacgata tccgcatcgg aaatccggtt
     1561 gataatgcgg taaaactgtc tggcgaacca gctccggatc ggcggttctc cctcgcggtt
     1621 gaccctgcgt gtcgccacac tgtcgaactc tccggatgta agcatcgcaa acatctgcgg
     1681 aagaagtgcc ggcggatcct gcatatccgc atccatcact gcaacatagt cccccctcgc
     1741 attcgtaaat ccggcataca ttgccgcttc tttgccaaaa tttcttgaaa aagagaggta
     1801 ggtaacatgc tcatcttttt ctgccagtcc ctttaagata gacaatgtcg cgtcttttga
     1861 cccgtcattg acaaacagaa actcgtattc acaattcatc cccgcgagaa cttttgtaga
     1921 ctcctcataa aaaatcggca atgcttcctg ctcgttgtag cagggaacaa ttagtgaaat
     1981 caacatttta ttttccttt
//
LOCUS       DBMDQJ010000466         1998 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19317_length_1998_cov_1.470216, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000466 DBMDQJ010000000
VERSION     DBMDQJ010000466.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19317_length_1998_cov_1.470216"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..824
                     /locus_tag="ACZO9R_09210"
     CDS             <1..824
                     /locus_tag="ACZO9R_09210"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR0913276.1"
                     /translation="GDSLIIVATDRISAFDHILKNKITKKGAILTQMSKFWFDYTKDI
                     VPNHMISVDVKDMPEFFRQERFEGNSMMCRKLEMLPIECIVRGYITGSGWASYQENGT
                     VCGIKLPEGLVESDKLPEPIYTPSTKAEIGLHDENISFEESVKVLEKIYPGKGADYAA
                     QIKDKTIALYKKCAEYALSKGIIIADTKFEFGLDENGNVVLGDEMLTPDSSRFWPLEG
                     YEAGKGQPSFDKQFVRDWLKANPDNDLLLPDEVVEKTVAKYKEAFELLTGKPFQA"
     gene            861..>1998
                     /gene="purF"
                     /locus_tag="ACZO9R_09215"
     CDS             861..>1998
                     /gene="purF"
                     /locus_tag="ACZO9R_09215"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR0913277.1"
                     /translation="MSDNRINKNQPVGDELHEECGVFGMYDFDGGDVASTIYYGLFAL
                     QHRGQESCGIAVSETNGPKGKVTSYKGMGLVNEVFTEENLEPMHGDIGVGHVRYSTAG
                     ASTRENAQPLVLNYVKGTLALAHNGNLINAAELRHDLEYTGAIFQTTIDSEVIAYHIA
                     RERLKSSTVEEAVGRACRKIKGAYSLVVMSPRKLVGARDPYGFKPLCIGKRDNAYILA
                     SETCALDTIGAEYVRDVLPGETVTITPENGIVSDMSRALPKEQEARCIFEYIYFARPD
                     SHIDGVSVYASRIKAGRFLAMDSPVDADLVVGVPESGNAAALGYSLQSGIPYGTAFVK
                     NGYVGRTFIKPKQSSRESSVRVKLNVLKEAVDGKRIIMIDDSIVH"
BASE COUNT          531 a          440 c          600 g          427 t
ORIGIN      
        1 atggagacag tctgattatt gtagccacag acagaatctc agcttttgat catattttaa
       61 agaataagat cacaaagaag ggcgcaattc ttacccagat gtcgaagttc tggttcgatt
      121 acaccaaaga tatcgtgccg aaccacatga tttccgtgga cgtaaaagat atgccggaat
      181 ttttccggca ggaacgtttt gagggaaaca gcatgatgtg ccggaagctt gagatgcttc
      241 cgatcgagtg catcgtgcgc ggatatatta caggaagcgg ctgggcaagc tatcaggaga
      301 acggcaccgt atgcggcatt aagctgccag aaggactggt agagtccgac aagctgccgg
      361 agccgatcta cacaccgagc acaaaggctg agattggtct gcatgatgag aacatctcct
      421 ttgaggaaag cgtgaaagtg ctggagaaga tctacccggg caaaggtgcg gattacgcgg
      481 cacagattaa ggacaagacg attgctcttt acaaaaagtg tgcagagtac gcactgagca
      541 agggcatcat tatcgcagat accaaattcg agttcggtct ggatgagaac ggcaacgttg
      601 ttctgggtga cgagatgctg acaccggaca gctccagatt ctggccgtta gagggatatg
      661 aagccggaaa aggtcagcct tcctttgaca agcagtttgt cagagactgg ttaaaggcaa
      721 atccggacaa cgatctgctt ctgccggatg aggtggtaga gaagaccgtg gccaagtaca
      781 aggaggcatt tgagcttctt accggaaagc cgtttcaggc ataaaaagac agaatatcag
      841 attcgtagat ggggaaatca atgagcgata atagaatcaa taaaaatcag ccggtggggg
      901 atgaactgca tgaggagtgc ggcgtcttcg gaatgtacga ttttgacggc ggagatgtgg
      961 catccacaat atattacgga ctgtttgcat tgcagcaccg cggacaggag agctgcggta
     1021 ttgcagtcag cgagacgaac ggtccgaaag ggaaagtgac ctcctacaag ggaatgggac
     1081 tggtcaatga ggttttcact gaggagaatt tagaacccat gcacggggat atcggcgtgg
     1141 ggcatgtgcg ctattcgaca gccggtgcgt cgaccagaga aaacgcacag ccgttggtgc
     1201 tcaattatgt caagggaacc ctggctctcg cgcacaacgg taacctgatc aatgcggcgg
     1261 aactcagaca tgatctggag tatacaggag ctattttcca gacgaccatt gactctgagg
     1321 tcattgccta ccatatcgca agggagcgtc tgaaatccag cacggtggag gaagcggtgg
     1381 gacgtgcctg tcgcaagatc aagggagcgt attcccttgt ggtcatgagc ccgagaaagc
     1441 tggtcggagc aagggatccc tatggtttta agccgctttg catcggaaag cgcgacaatg
     1501 cctatatcct tgcatcggag acctgtgcgc tcgatacgat cggcgcggag tatgtgcgtg
     1561 atgtgctgcc gggagagacg gtgacgatca cgccggagaa tggaattgtc tcggatatgt
     1621 cccgcgcact gcccaaggag caggaagcga gatgcatttt cgagtacata tattttgcga
     1681 gaccggacag tcatatcgac ggcgtgagcg tatatgcatc ccgcatcaag gcaggacggt
     1741 tcctggcgat ggattcgccg gtggatgcag atcttgtggt gggcgtgccg gagtccggca
     1801 atgccgctgc gctcggttat tcactgcagt ccggcatccc gtacggaacc gcttttgtca
     1861 aaaacggcta tgtgggacgt acgtttatca agccgaagca gagcagccgt gagtccagcg
     1921 tccgcgtgaa gctgaacgtc ttaaaagagg cggtggacgg caagcgcatt atcatgatcg
     1981 atgactcgat tgtgcacg
//
LOCUS       DBMDQJ010000467         1992 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19398_length_1992_cov_1.812269, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000467 DBMDQJ010000000
VERSION     DBMDQJ010000467.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19398_length_1992_cov_1.812269"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="ACZO9R_09220"
     CDS             <1..1285
                     /locus_tag="ACZO9R_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0913278.1"
                     /translation="LSQERLANLQKELAELRDEFNTKKAQWENEKKSVEKVQKLREEI
                     EAVKNEIKMAQQKYDLEKAAELQYGKLPQLQKQLEIEEEAVKKRDLSLVHENVSEEEI
                     ARIISRWTGIPVAKLTESERNKTLHLDEELHKRVIGQDEGVTKVTEAIIRSKAGIKDP
                     SKPIGSFLFLGPTGVGKTELAKALAASLFDDESNMVRLDMSEYMEKYSVSRLIGAPPG
                     YVGYDEGGQLTEAVRRKPYSVVLFDEVEKAHPDVFNVLLQVLDDGRITDSQGRTVDFK
                     NTIIIMTSNLGSAHLLEGIDANGDINPECEAAVMDELRGHFRPEFLNRLDEIIMFKPL
                     TKDNIGNIINLLVADLNKRLADREITVELTDAAKQYIVENGYDPIYGARPLKRFLQKH
                     VETLSAKLILADKVREGDTILIDVSGNELTAAVKA"
     gene            1588..>1992
                     /locus_tag="ACZO9R_09225"
     CDS             1588..>1992
                     /locus_tag="ACZO9R_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat protein"
                     /protein_id="HIR0913279.1"
                     /translation="MKNRKIMIAGMLICAITAGCGTALPHADGMGETERETKEAQLNS
                     SDETEKIQSIAQAEEQTENSDVEDADDQLAVIARTRNQWVNDPDYADEMYQFAIADLD
                     HDGRLELIVSNCGGTGIYTYSRFYRVEENGTLK"
BASE COUNT          595 a          393 c          596 g          408 t
ORIGIN      
        1 tctgagccag gagcgtctgg caaatctgca gaaagaactg gcagagctgc gcgatgaatt
       61 caacacgaag aaagcgcagt gggagaatga gaaaaagtcg gtagaaaaag tacagaagct
      121 gcgtgaggag atcgaagcgg tcaagaatga gatcaagatg gcacagcaga agtacgatct
      181 ggagaaggca gccgaattgc agtacggcaa gctgccacag ctacagaagc agttggagat
      241 cgaggaggaa gccgtgaaga aacgcgacct ttccctggtg catgaaaacg tcagtgagga
      301 agagattgcg cggatcatct ccagatggac aggaattccg gtggcaaagc tgacggagag
      361 cgagcgtaac aagacgctgc atctcgacga ggaactgcac aaacgtgtca tcggccagga
      421 tgagggcgtc acgaaagtga cggaagcaat catccgttcc aaagcaggta tcaaagaccc
      481 gagtaagccg atcggatcat tcctgttctt aggaccgacc ggtgttggta agacagagct
      541 tgcaaaggcg cttgcagcaa gtctcttcga tgatgagtcg aatatggtgc gtcttgatat
      601 gagtgagtat atggagaaat attccgtctc ccgtctgatc ggagcgcctc caggatatgt
      661 cggatacgat gagggtggac agctgaccga ggcagtgcgc agaaagccgt attcggtggt
      721 attgtttgat gaggtggaga aggcacatcc ggatgtgttc aacgttctgt tgcaggtact
      781 ggacgatgga cgaatcacag attcacaggg acgtaccgtc gattttaaga atacaatcat
      841 catcatgaca tcgaaccttg gatcagcaca tctgcttgag ggcatcgatg caaacgggga
      901 tatcaatccg gagtgcgaag cggcagttat ggacgaactg cgcggacatt tccgtccgga
      961 gtttttgaac cgtctggatg agattattat gtttaagccg ctgacgaagg acaacatcgg
     1021 taacatcatc aaccttctgg ttgcagatct caacaaacgt ctggcagacc gtgagatcac
     1081 ggtggagctg accgatgcgg caaagcagta tatcgtggaa aacggatatg atccgatcta
     1141 cggtgcaaga ccgttaaagc ggttcttaca gaagcatgtg gagacacttt ccgcaaagct
     1201 gattcttgcg gataaagtca gggagggaga tacgattctg attgatgtct ccggaaatga
     1261 actgacggca gcagtgaaag cgtgagggtt gatctaaaac aggaacagag aagagggacg
     1321 gaggcagaaa gcctccgccc ctttttttgt gtaataggga acttcgttcc tattacacaa
     1381 aaacgctccg cgaggatgcg cacctcgcgg tgaggtaaat aatgctttgc ataccgcgag
     1441 ggcgcgcaag tgtgcggcaa gcgcacacgt tgttcttgag tgaaacgaaa gattgaaaaa
     1501 tgcctgtgaa gtcattaaaa taaaacagat actacatatt ttaaaaggat agataccgat
     1561 gtatttttca aaagtgggac aggagcgatg aaaaacagga agatcatgat agcaggaatg
     1621 cttatctgtg cgataactgc cgggtgcggg acggctttac cgcacgcaga cgggatggga
     1681 gagacggagc gagagacgaa ggaagctcag ttaaattcct cagatgagac agagaagata
     1741 caaagtattg cacaggcgga agagcagaca gaaaattccg atgtggagga tgcggacgat
     1801 cagcttgccg tcattgcgcg gacaaggaat cagtgggtga atgatccaga ttacgcagat
     1861 gagatgtatc agtttgcgat agcagatctt gatcacgatg gcagactgga gctgattgta
     1921 tcgaactgtg ggggaaccgg aatctatacg tacagcaggt tttacagggt ggaggagaat
     1981 ggcacgttaa ag
//
LOCUS       DBMDQJ010000468         1990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19435_length_1990_cov_1.385389, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000468 DBMDQJ010000000
VERSION     DBMDQJ010000468.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19435_length_1990_cov_1.385389"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="ACZO9R_09230"
     CDS             complement(<1..710)
                     /locus_tag="ACZO9R_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HIR0913280.1"
                     /translation="MKKKSYISTKFIILIPVFILGFVSVVSNILAVTNIKRVNANATQ
                     IANGYMSCISELGDIQKETLLIHRLGLSHIVATDLNTMISLVETIRSEQETLETYLDD
                     FQEYVTDDNKAEYDALVSNYEGMKYELANLMAYSANNDNEAAYALANGKIADYSSAMQ
                     ESIAAIRTNVSENADVAKEQLAAVYRTAIAASTASIIISVAALLATLICVFQLVIIPL
                     LKTQKEITDIIEDIDKREG"
     gene            complement(727..>1990)
                     /locus_tag="ACZO9R_09235"
     CDS             complement(727..>1990)
                     /locus_tag="ACZO9R_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR0913281.1"
                     /translation="AGCTGADTTAGGESVNGEPSVLQTEAVMDSTELSGDVAEDDGKV
                     TLTVWAEEANFDVLQEMIDSFEQKYAGQADFDIQLAENADAETRNTLLGDVHNGADVF
                     PLPDDQLTSMVAAGALEPVPNADEIREANLDEAVAAASVNDTLYAYPMTADNGYFLYY
                     DKNYLTEEDVQTMDGLLAAAGAVGKKVTMDWSSGWYLYAFFGNTGLDFGVNDDGVTNY
                     CDWNATEGSIKGTDIEEALLAIAQNPAFLSCTDTEFMEGVQDGTVVAGVSGVWNASEI
                     KKVWGNDYGAVKLPTYTCAGQQIQMASFTGYKMMGVNAYSKHKDWALKLADWFTNEEN
                     QMLRLEERDQGPSNKNAAASEQVQAVPAIQAVIAQAQYGKLQRVGNSFWDATTEFGNL
                     MATGSTNGTDPQEVMDTLVAGITQSTVQ"
BASE COUNT          404 a          614 c          444 g          528 t
ORIGIN      
        1 ccctcgcgct tgtcaatgtc ctcaataata tctgtgatct ccttctgtgt ctttaacaga
       61 gggataatta ccagctggaa tacgcagatc agagttgcaa gcagcgccgc cacactgatg
      121 atgatggatg ccgtgctcgc tgcgattgcc gtccggtaga ccgctgcaag ctgttccttt
      181 gccacatccg cattttcgct gacattggtg cggattgccg caatgctctc ctgcattgca
      241 ctggaataat cggcgatctt gccgtttgcc agcgcataag ccgcctcgtt atcgttgttg
      301 gcactgtatg ccatgaggtt ggcaagctcg tacttcattc cctcgtagtt ggacaccagc
      361 gcatcgtatt ccgccttgtt gtcgtctgtc acatattcct ggaaatcatc cagatatgtc
      421 tctaaggtct cctgctcact ccggatggtc tcgaccagcg agatcatggt atttaagtcg
      481 gtcgccacga tatgggacag tcccagtctg tggatcagaa gcgtctcttt ctgaatatcg
      541 ccgagctctg aaatacagct catgtagccg tttgcaatct gcgttgcatt tgcatttact
      601 ctcttaatat tggtcactgc cagaatattg gaaacgactg acacaaatcc caggataaat
      661 accggaatca gaataataaa ctttgtactg atatagcttt ttttcttcat atcctgctcc
      721 tccccgttac tgtaccgtcg actgtgtaat tccagccaca agcgtatcca tcacttcctg
      781 cggatccgtt ccgtttgtgc tgcctgtcgc catcagattg ccgaactccg tcgtcgcatc
      841 ccagaagctg ttgcccacac gctggagttt gccgtactgc gcctgcgcaa tgactgcctg
      901 gatggcaggc accgcctgta cctgctccga cgcagccgcg tttttattgg aaggtccctg
      961 atcgcgctcc tcaagacgca gcatctggtt ctcctcgttt gtaaaccagt ctgcaagctt
     1021 taatgcccag tccttgtgct tggaataggc gttgacgccc atcatcttat agcccgtgaa
     1081 agaggccatc tggatctgct gtcccgcgca ggtgtaggtc ggaagcttca ctgccccgta
     1141 atcgttgccc catacctttt tgatctccga tgcattccac acgccgctga cgcctgccac
     1201 aaccgttccg tcctgcacgc cctccatgaa ttccgtgtcg gtacagctta aaaacgccgg
     1261 attctgtgcg atggcaagca gcgcctcctc aatgtcggtt ccctttatgc tgccttccgt
     1321 cgcattccag tcgcagtagt tcgtcacccc gtcgtcattt acaccgaaat ccagtccggt
     1381 gttgccgaaa aacgcataga ggtaccagcc ggaactccag tccatcgtca ccttcttgcc
     1441 cacagcgccc gctgctgcta gaagcccgtc catcgtctgg acgtcctctt ccgtcaggta
     1501 attcttgtcg taatacagaa aatatccgtt gtccgccgtc atcgggtaag cgtacagcgt
     1561 atcattcaca gaagccgctg caaccgcctc atccagattc gcctcccgga tctcatctgc
     1621 atttggtacc ggctctaagg caccggctgc aaccatgctg gtaagctgat cgtccggcag
     1681 cgggaagaca tccgctccgt tatgtacatc tccaagcagc gtattgcgcg tctcagcatc
     1741 tgcattctcc gcaagctgaa tatcaaaatc tgcctgtcct gcatatttct gctcgaagct
     1801 gtcgatcatc tcctgcagaa catcaaaatt ggcctcctct gcccatacgg tcaatgtaac
     1861 cttgccgtca tcctccgcta catcgccgga caactctgtc gaatccatga ccgcctccgt
     1921 ctgcaatacg gacggttcac cgtttacgga ctctccacct gccgtcgtat ccgcacctgt
     1981 acagcccgcc
//
LOCUS       DBMDQJ010000469         1990 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19440_length_1990_cov_1.132345, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000469 DBMDQJ010000000
VERSION     DBMDQJ010000469.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19440_length_1990_cov_1.132345"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            115..972
                     /locus_tag="ACZO9R_09240"
     CDS             115..972
                     /locus_tag="ACZO9R_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913282.1"
                     /translation="MNWLNKLERKFGNWAIPNLIVWLIGAYTIGFVLYQVNPSIIGLL
                     MLSPYHILHGQIWRLITWVFMPTDTNLVYLLIMALFYYQLGTTLERTWGTFRFNVYIF
                     GGMLFTVIGAFLLYGIMYLMNGGVTTEMLLIGTSFSTSYINMSIFLAFACMYPDMQVL
                     LMFIIPIKMKWMAAVYGVLIALSFFETGWAGRMAIFMSLLNFIIFFFSTRNLKRYTPH
                     EVHRRQKFKADMRRPQGYAGGAKHKCAVCGRTELDDPTLEFRFCSKCEGNYEYCQDHL
                     FTHQHIRMS"
     gene            complement(1048..>1990)
                     /gene="aroC"
                     /locus_tag="ACZO9R_09245"
     CDS             complement(1048..>1990)
                     /gene="aroC"
                     /locus_tag="ACZO9R_09245"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR0913283.1"
                     /translation="RYATPRKEDDAVEILSGVFEGKTTGTPISLVVFNRNQQSKDYSE
                     IASYYRPGHADYTFDQKYGFRDYRGGGRSSGRETIGRVAAGAVAVKILNELGITFITY
                     TKSIGPIAVSSDRFNFSEVTKNPLYMPDAEAAENAENYLDACMAELNSSGGIVECIIK
                     GMPAGIGDPVFEKLNANLAKAVMSIGAVKGFEIGDGFDVAKATGKNNNDAFVLGADGR
                     IAKATNHAGGILGGMSDGSDIILRAAIKPTPSIAATQRTVNQSGEEISVNIKGRHDPI
                     IVPRAVVVVESMAAITIVDALFSNMSARMDALKDFYS"
BASE COUNT          450 a          472 c          548 g          520 t
ORIGIN      
        1 gccggaaaag tataaacttt ttgtaaagaa gaatccctct gattgacaaa gatttccccg
       61 accgatatac tgaacaacgt aatgtcattt ttggaaaggt caggaacaga gtgtatgaac
      121 tggttaaata aattagaacg taaattcggc aattgggcga ttcccaatct gatcgtgtgg
      181 ctgatcggag cttacacgat cgggtttgtg ctctaccagg tgaatccgtc gatcatagga
      241 cttttgatgt tgagtccgta tcacatcctg cacggacaga tctggcgtct gattacctgg
      301 gtgtttatgc ccacagacac gaatctggtg tatcttctca ttatggcgct tttttattat
      361 cagcttggaa cgacactgga gcgcacatgg ggaaccttcc ggtttaacgt ctatattttc
      421 ggaggtatgc tgtttaccgt catcggagcg tttctgctgt acggtatcat gtatctgatg
      481 aacggcggtg tgacgacgga gatgcttctg attggaacaa gcttcagcac aagttacatc
      541 aacatgtcca tcttccttgc gtttgcatgt atgtatccgg atatgcaggt tcttctgatg
      601 ttcattatcc cgattaagat gaagtggatg gcggctgtgt acggcgttct gattgcttta
      661 agcttttttg agaccggatg ggcgggaaga atggcgattt ttatgtcgct cctcaacttt
      721 attattttct tcttctccac cagaaacctc aagcgctata cgccgcacga ggtgcacaga
      781 cggcagaagt ttaaggcaga catgcgaaga ccgcagggat atgcgggcgg tgcgaagcac
      841 aagtgtgctg tctgcggcag gacggagctt gacgatccga cgctggaatt ccgtttctgc
      901 tccaagtgcg agggcaatta cgaatactgt caggatcatc tgtttacaca tcagcatatt
      961 cggatgagct agaaagtatg aaaaagaaaa aggtaatact tcgattgcgc cagccggcgg
     1021 agaagaagta tcaccttttt gtttctttta ggagtagaaa tcttttagcg catccatccg
     1081 tgcactcata ttggaaaaaa gtgcgtcgac gatcgtgatg gctgccatgg attccaccac
     1141 gacaacggcg cgcggaacga tgattggatc gtggcgtccc ttgatgttga ccgagatctc
     1201 ctccccggac tgattgacag tgcgctgtgt ggcggcgatg gaaggggtcg gcttgatggc
     1261 agcccgaagg atgatgtcgc ttccgtcgct catgccgcct aagataccgc cggcatggtt
     1321 ggttgctttg gcaatcctgc cgtccgcacc gaggacaaag gcatcgttgt tgttcttgcc
     1381 ggtcgctttt gctacgtcaa atccgtcccc gatctcaaaa cctttgacgg caccgatgga
     1441 catgaccgcc ttggcaaggt tcgcattaag cttctcgaag accggatctc cgatgccggc
     1501 gggcatcccc tttatgatac actccacaat gccgccggag gaattcagtt ccgccatgca
     1561 ggcatccaga tagttttcgg cgttctcggc tgcctcggcg tccggcatat agagcggatt
     1621 cttcgtgact tcggaaaaat taaaacgatc ggaagatacg gcgatcgggc cgatggattt
     1681 tgtgtaggtt ataaaggtaa tgccgagctc gtttagaatc ttgacggcga cggcgccggc
     1741 ggcgacacgc ccgatggttt cccgcccgga ggagcgcccg cccccgcggt agtcgcggaa
     1801 gccgtatttc tggtcgaacg tgtaatcggc gtgaccggga cggtaatagg aggcaatctc
     1861 gctgtagtct ttggactgct ggttgcggtt gaagacgaca agcgagatcg gggtgccggt
     1921 cgtctttcct tcaaacacgc cagagagaat ctctacggcg tcgtcctctt tgcgcggcgt
     1981 tgcatatctg
//
LOCUS       DBMDQJ010000470         1984 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19513_length_1984_cov_1.296336, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000470 DBMDQJ010000000
VERSION     DBMDQJ010000470.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19513_length_1984_cov_1.296336"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="ACZO9R_09250"
     CDS             complement(<1..1321)
                     /locus_tag="ACZO9R_09250"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HIR0913284.1"
                     /translation="MLKTAKIYGINGPVIYLKGNTGFRMSEMVYVGEQKLVGEVIALD
                     KDMTTVQVYEETTGLRPGEEVIASGSPVSVTLAPGILNNIFDGIERPLEDIAESSGGA
                     FITRGVSVDSLDRTKKWKTHITVKQGDYLHAGDIIAEVPETHAITHKCMVPPEVEGTV
                     LVTVADGEYTIDEPLVRLELPNGQEKELTMTQHWPIRTPRPTHHRFPASVPLVTGQRI
                     IDTMFPIAKGGTAAIPGGFGTGKTMTQHQIAKWADADIIIYIGCGERGNEMTQVLEEF
                     SELTDPRTGKPLMDRTTLIANTSNMPVAAREASIYTGLTLAEYYRDMGYDVAIMADST
                     SRWAEALRELSGRLEEMPAEEGFPAYLASRLSAFYERAGMMHNLNGTDGSVTIIGAVS
                     PQGGDFSEPVTQNTKRFVRCFWGLDKSLAYARHFPAIHWLTSYSEYLN"
     gene            complement(1314..1901)
                     /locus_tag="ACZO9R_09255"
     CDS             complement(1314..1901)
                     /locus_tag="ACZO9R_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HIR0913285.1"
                     /translation="MTLEEKIAHLQTTSMEQARLEGNVIIDTHQAALEKVFEDHKAEA
                     TRQSETRIKAEAANAKLSLNQALAKSQLEIKRRQSKVQQELKDKIFEEADALIDEFMK
                     TEAYDDFLIKCIRKAVAFAGNDELTIYINPTDEKKRSDLEGATRVHLTISAEDFRGGV
                     RAVIRSRNILIDNSFSTQLKEQYDKFVFLGGDGIA"
BASE COUNT          462 a          486 c          486 g          550 t
ORIGIN      
        1 cgttcagata ctcactgtaa ctggtcagcc agtggatggc cgggaaatgt cttgcatagg
       61 caaggctctt atccagtccc cagaagcagc ggacgaatcg tttggtgttc tgtgtaaccg
      121 gctcggagaa gtcaccaccc tgtggggaaa cagcaccgat gatcgtaaca gaaccgtctg
      181 ttccgttcag gttatgcatc attccggcgc gctcgtagaa cgcagacaga cgagatgcga
      241 ggtaagccgg gaatccttcc tcggcaggca tctcttccag acgtccggaa agctctcgaa
      301 gagcctcagc ccaacgggaa gtggagtcgg ccatgatcgc tacatcgtag cccatgtcac
      361 ggtaatattc tgccagagtg agtccggtgt aaatgctggc ttcacgggca gcaacaggca
      421 tgttggaagt attggcaatc agtgtagtac ggtccatcag aggtttgccg gtacgaggat
      481 cggtcagttc gctgaactct tccaaaacct gtgtcatctc atttccacgc tcaccgcatc
      541 cgatatagat gatgatatct gcatcggccc atttcgcaat ctgatgctgt gtcatggtct
      601 ttccggttcc gaatcctcca gggatggcag cggtaccgcc ctttgcaatc gggaacatgg
      661 tatcgatgat acgttgtccg gtaacaagcg gaacagatgc cgggaaacgg tgatgagtcg
      721 gtcgcggagt acggatcggc caatgctgag tcattgtcag ttctttctcc tgtccgttag
      781 gaagttccag acgaaccaga ggttcgtcaa ttgtgtattc accgtctgca acagtcacaa
      841 gaacagttcc ctctacttca ggaggaacca tacatttatg agtgatcgca tgtgtttcag
      901 gaacttctgc gataatatca cctgcatgga gataatctcc ctgctttaca gtgatgtgtg
      961 ttttccattt ctttgtacgg tccagagaat cgacactgac accgcgtgtg ataaatgcgc
     1021 cgccggagga ttctgcgata tcttccagtg gacgttcgat accatcaaaa atgttgttca
     1081 ggataccggg tgcaagtgtt acggatacag ggcttccgct ggcaataact tcttcgcccg
     1141 ggcgaagccc cgttgtttcc tcataaacct gaacggttgt catatcttta tccagtgcaa
     1201 tgacttctcc gactaacttc tgttcgccga catagaccat ctcggacata cggaatcctg
     1261 tgttgccttt cagataaatg acgggaccat tgatgccgta aattttcgct gtcttaagca
     1321 atgccgtcac ctcctaaaaa tacaaatttg tcatactgtt ctttcagctg tgtgctgaag
     1381 gaattatcga tcaaaatgtt gcgggaacgg atgacagcac ggacaccgcc ccggaaatct
     1441 tcggcgctga tcgtcagatg aactcttgta gcaccttcca gatcagaacg ttttttttca
     1501 tcagttgggt taatatagat cgtaagctca tcatttccgg caaaagccac cgctttgcgg
     1561 atgcatttga taagaaaatc atcataagct tccgtcttca taaattcatc gattaatgcg
     1621 tctgcttcct cgaagatctt gtctttcaat tcttgctgaa ccttgctttg acgacgtttg
     1681 atctccagct gggacttggc gagcgcctga ttcagtgaga gcttcgcatt ggcagcttcc
     1741 gccttgatgc gagtttctga ctgtcttgtg gcttctgcct tatgatcttc gaaaaccttc
     1801 tccagggcag cctgatgggt gtcaatgatg acatttcctt ctaagcgggc ctgctccatg
     1861 gatgtggttt gcaggtgtgc gattttttct tccagagtca agaggaatcc ctcctttagt
     1921 tcgtaatata attgcgttta taatttcagt ccgatcgcct cagtgatata tgaggtgata
     1981 aagt
//
LOCUS       DBMDQJ010000471         1982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19526_length_1982_cov_2.256247, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000471 DBMDQJ010000000
VERSION     DBMDQJ010000471.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19526_length_1982_cov_2.256247"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..130)
                     /locus_tag="ACZO9R_09260"
     CDS             complement(<1..130)
                     /locus_tag="ACZO9R_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR0913286.1"
                     /translation="MASQVMRITLKAYDHKLVDSSAQKIIETVKKNGSQVSGPVPLP"
     gene            complement(443..>1982)
                     /locus_tag="ACZO9R_09265"
     CDS             complement(443..>1982)
                     /locus_tag="ACZO9R_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913287.1"
                     /translation="LLVWAVVLCVLSRLRSNLFLIIGQIGVGISLILGVLMCNWTEDA
                     ERFLILSIYYAVTQTIFFVSHLRREYYRNAVNLVSWSIGLLILVLGAAASYTEGSAAG
                     NVSVVLLLALAAVPVLLGVAAAEMDENGKLLGGIVGALCIIIFCGLFLNRFTGAALLL
                     LVFGIAVLALLEFRRPGDLSAGTVLLEVAVFAQMFFAGAAAPYFGEYISTGLLAEACL
                     LYGFLRKRKAYRAAALGYGILLLLIPMNGGMRTVWGLVFFCSAVLLIRFCREQYETWM
                     KICGYLMFLAFLSETAVWGTDLAAVADVYLSDLICFMPIVLCNILAAKVPLFCRNPKT
                     GEEEKAFAAVTMAVQALLIAAVLVLMGKPDAPVLHLIYVLLGLVLVCTNTYSLLIRKD
                     GDWWGIYVALKLLLFASVALHSYGVPVFVNDVTGIVIAFCCIVAGFLAEMRMKRKYKA
                     LRVCGLVLVLLCVVKLVLVDIRYDNVLLRAAGFFVGGILCFGISLVYNLVDKWMGRRE
                     NAKN"
BASE COUNT          542 a          622 c          413 g          405 t
ORIGIN      
        1 taggaagagg taccggtccg ctcacctgtg atccgttctt ctttacagtt tcgatgattt
       61 tctgtgctga tgaatcaact aacttgtgat cataagcctt cagagtgatt ctcattactt
      121 gacttgccat aaaaaaagtc gcctcctttt cgcactttta caagcgatgt aatcgctttt
      181 tgtacgacaa gcggtgactg cacacatacc cgcgtgtatc atggactcac gcccacaaag
      241 aagcccgcgc ttcctacacg ccatccctct gtccttcgga caaattctat tcgtgataca
      301 gtgacttgtc gccagtttcc taacttgaca ttcgctccac ggaaaacctg caatgctctg
      361 cagcaacctc acgcttcaac gctatcaatg tcacagcaat gcatagtata cacgatgaaa
      421 ccgcgaattg caaggatttt tttcagtttt ttgcattttc gcgccgcccc atccacttgt
      481 cgaccagatt gtatacaaga ctgataccaa agcaaaggat gcctccgaca aaaaatcccg
      541 ccgcccggag cagcacattg tcgtagcgga tgtccacgag caccagtttt acaacgcaca
      601 gcagcaccag aaccagtccg cagacccgca gcgccttata ttttctcttc atccgcattt
      661 ccgccagaaa tcccgccacg atacagcaga acgcaatcac aatgcctgtc acatcgttca
      721 cgaacacggg caccccatag gagtgcagtg cgaccgatgc aaacagcagc agctttaacg
      781 ccacgtaaat tccccaccag tctccgtcct tccttatcag caggctgtag gtattggtgc
      841 acacgagcac cagtcccaaa agcacataga taaggtgaag caccggcgcg tccggttttc
      901 ccatcagaac cagaaccgct gcaataagga gcgcctgcac ggccatcgtt accgccgcga
      961 atgctttttc ttcctctccc gtctttggat ttctgcaaaa cagcggaact ttcgccgcca
     1021 gaatattgca gagcacaatc ggcataaaac agatcagatc gctcaggtaa acatctgcga
     1081 ccgctgccag atccgttccc cacaccgccg tttctgacag gaatgccaaa aacatcagat
     1141 aaccacagat cttcatccag gtttcatact gttccctgca gaaccggatc agcagcaccg
     1201 cgctgcagaa aaataccagt ccccagaccg ttctcatgcc cccgttcatc gggatcagca
     1261 acagcaggat gccatagccg agtgccgccg cccggtatgc ttttcttttg cgcaggaaac
     1321 cgtacagaag gcacgcctcc gccagaagtc ccgtcgatat atactccccg aaatacggcg
     1381 cggcagctcc cgcaaagaac atctgcgcaa aaacagccac ctccagcaga accgtacccg
     1441 cggacagatc cccggggcgc cggaattcca gcagcgccag aactgcaatg ccaaatacca
     1501 gtaagagcag cgccgctccg gtaaaccggt tcaaaaacag tccgcaaaaa atgatgatac
     1561 acagtgcccc cacaataccg ccaagcagtt ttccattctc gtccatttcc gccgcggcta
     1621 cgccaagcag taccggaacc gctgcaagcg caagaagcaa taccactgac acgtttcccg
     1681 cagcggagcc ttctgtatag gaagcagctg ctccaagcac cagaatgagc agcccgatgc
     1741 tccacgacac aagattcacc gcattacggt aatattcccg ccgtaggtgg gacacgaaaa
     1801 agatggtctg cgttaccgca taatatatag aaagaatcag aaacctttcc gcatcctccg
     1861 tccagttaca catgagcacg ccgagaatca atgagatgcc cacaccaatc tgccctatga
     1921 taaggaagag attcgaccgc agacgtgaca gcacacacag aaccaccgcc cacaccagca
     1981 gt
//
LOCUS       DBMDQJ010000472         1982 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19529_length_1982_cov_1.687932, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000472 DBMDQJ010000000
VERSION     DBMDQJ010000472.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19529_length_1982_cov_1.687932"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="ACZO9R_09270"
     CDS             complement(<1..880)
                     /locus_tag="ACZO9R_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase family protein"
                     /protein_id="HIR0913288.1"
                     /translation="MKNLNRSYYNPPFSHIYVEREVAEDPETKRILTQFPQAEVIAID
                     HYKDVFCRSRQDYVRQHAAQNLILAAKHGELLYPGAPVCQNFGNEHFYYTSCVMNCVF
                     DCEYCYLKGMYPSANLVVFVNLEDIFAEVEKRLAAHPVYLCVSYDTDLLALEHLTGYA
                     ARWIEFVRRVNADGQRLRIELRTKGTGGCLWQTVRPEAGVICAFTISPQQVIDAYEHG
                     TASLAGRLAGAEAAMEHGFSVRLCFDPMIYCSDWKKHYDAMFAQVCEVLDLEKLADVS
                     VGTFRISQDYLKKMRKN"
     gene            complement(852..1748)
                     /locus_tag="ACZO9R_09275"
     CDS             complement(852..1748)
                     /locus_tag="ACZO9R_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913289.1"
                     /translation="MIYVMMALYQEAHGLIRELELKKNTAYAPFEVFDNESAGIRLVV
                     TGVGEIAAAAATAAVCARDGADAADFLVNIGCCAAGGCEPADRDMDSGYGAAHAAQIG
                     DLYVCHKITEQATGKTFYPDILYRHPWKERELVTGMQPLQRAAAHGALYDMEAAAVYQ
                     AGIRFFSPDRMSFLKVVSDSGVAGQERMTAETLAGLLEQHVKAVAGFLANLWKAADEE
                     EALRSGGILQEDEAVLEQIFAALHCSQTMRASARQYITYAALTGYDWKVELEEWYARG
                     LLPCKDRREGKVRLEELKQVLL"
     gene            complement(1758..>1982)
                     /locus_tag="ACZO9R_09280"
     CDS             complement(1758..>1982)
                     /locus_tag="ACZO9R_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR0913290.1"
                     /translation="GDAVTAAVKKMLKDPACGHIFRVKGFLQNPDGTWLEINATQQEI
                     TRKPIANGQDVLIVIGENLVEDTIRAYWKG"
BASE COUNT          412 a          675 c          448 g          447 t
ORIGIN      
        1 catttttccg catcttcttc agataatcct gggagatgcg gaaggttccg acgctgacat
       61 ccgccagttt ttccaggtcc aacacctcgc agacctgcgc aaacatcgcg tcatagtgct
      121 tcttccagtc gctgcaatag atcatcggat caaagcagag acgcaccgaa aagccgtgtt
      181 ccatcgccgc ttccgctcct gccagccgcc cggcgagcga agccgtaccg tgctcatatg
      241 catcaatcac ctgctgcggt gagatggtaa acgcacagat gacgcctgcc tccggccgga
      301 ccgtctgcca caggcagcct ccggttccct tggttctaag ctcgatgcgc agccgttgtc
      361 cgtcggcatt caccctgcgc acaaactcaa tccagcgcgc cgcatagccg gtcagatgct
      421 ctagtgccag cagatccgta tcgtaggaca cacacaggta gaccgggtgc gcggcaagtc
      481 tcttttccac ttccgcaaaa atatcctcca gattgacgaa caccaccagg ttggcggacg
      541 gatacattcc cttcagatag cagtattcac agtcaaacac gcagttcatc acacaggagg
      601 tgtagtaaaa atgctcattt ccaaagttct ggcagaccgg cgcgccgggg tagagcagct
      661 caccgtgctt cgccgccaga atcagattct gcgccgcatg ctgcctgaca tagtcctgcc
      721 tgctccggca gaaaacatcc ttatagtggt cgatagcaat cacttctgcc tgcggaaact
      781 gcgtgagtat ccgctttgtc tccggatctt ccgccacttc ccgctccaca taaatatggg
      841 aaaacggcgg attataataa gacctgttta agttcttcaa gccgcacctt cccttcccgc
      901 ctgtctttgc acgggagcag cccccgcgca taccattctt ccagttccac tttccagtcg
      961 tatccggtaa gcgccgcgta cgtgatatac tgccgcgccg acgcccgcat cgtctgcgag
     1021 cagtgcagcg ccgcaaaaat ctgctccagc accgcctcgt cttcctgcaa aatgccgccg
     1081 cttcgcagcg cctcttcctc atcggccgcc ttccacagat ttgcaaggaa tcccgcgacc
     1141 gcctttacat gctgctccag cagccccgcg agcgtctccg ccgtcattct ctcctgcccc
     1201 gcaactccgg agtccgatac caccttcaaa aagctcatcc ggtccggaga aaaaaagcgg
     1261 atgcctgcct gatagacggc cgccgcttcc atatcatata aagctccgtg tgccgctgct
     1321 ctttgcagcg gctgcatccc ggtcacaagt tcacgttcct tccacgggtg ccggtacaga
     1381 atatccggat agaacgtttt cccggtcgcc tgctccgtga tcttgtgaca cacgtacagg
     1441 tctccgatct gtgcggcgtg tgccgccccg tatccgctgt ccatgtcccg gtctgccggt
     1501 tcacagccac ctgccgcgca gcagccgata ttgacgagaa agtctgccgc atctgctcca
     1561 tccctcgcac agactgccgc ggtcgcagca gccgcagcaa tctcccccac accggtcaca
     1621 accagccgga tgcctgcgct ctcattgtca aacacctcaa acggcgcata cgccgtgttc
     1681 ttttttaatt ccagttcccg gatgagtccg tgtgcctcct gatagagcgc catcatcaca
     1741 taaatcatcg tctgtctcta acctttccaa tacgcccgga tggtgtcttc cacaagattc
     1801 tctccgatca caatcagaac atcctgccca tttgcaatcg gctttcgcgt gatctcctgc
     1861 tgtgttgcat tgatctccag ccatgtgcca tccggattct gcaaaaatcc cttcacacgg
     1921 aaaatatgtc cgcacgccgg atctttcaac atcttcttca ccgccgccgt aaccgcatct
     1981 cc
//
LOCUS       DBMDQJ010000473         1978 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19578_length_1978_cov_0.841769, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000473 DBMDQJ010000000
VERSION     DBMDQJ010000473.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19578_length_1978_cov_0.841769"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="ACZO9R_09285"
     CDS             <1..200
                     /locus_tag="ACZO9R_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008391076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HIR0913291.1"
                     /translation="HIEKAYADVRGAYPLINQQFLEHEVSGYKYVNREDDTGEEGLRT
                     AKLSYHPVFMVEKGIATLKGE"
     gene            207..1262
                     /gene="hisC"
                     /locus_tag="ACZO9R_09290"
     CDS             207..1262
                     /gene="hisC"
                     /locus_tag="ACZO9R_09290"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HIR0913292.1"
                     /translation="MSTWEENVRKVTPYTPGEQPNRPQMIKLNTNENPYPPAPGVAEV
                     LRHPDEDALRRYPDPAASVLVNALAENYHVKPEQVFVGVGSDDVLAMAFMTFFNSEKP
                     ILFPDISYSFYPVWAEEFRIPHTRVALDENFCIRKEDYYGENGGIVFPNPNAPTALAM
                     GLDDVEDIIRHNPDVVVIVDEAYVDFGAESALKLVEKYENLLVVQTFSKSRSLAGMRI
                     GFAIGSPKLIKYLNDVKYSFNSYTLNQTSLACGVEAVKDQTYFNETRTRIMKTREHAK
                     EVLGEMGFSFPDSKANFLFITHPDYPAKELFQALRQKDIYVRYFDQPRINNYLRVTIG
                     TDEQMEAFYEFLRDYMR"
     gene            1266..1769
                     /locus_tag="ACZO9R_09295"
     CDS             1266..1769
                     /locus_tag="ACZO9R_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009266440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG2426 family protein"
                     /protein_id="HIR0913293.1"
                     /translation="MMESIMSWVISALSGHVSREMIVFIISMVPILELRGGLLAASVL
                     NINITRALWICIIGNIIPVPFILLLITPIFNWLKQTKTFRPMVEKMEARALSKREQVE
                     KYEFWGLVLFVGIPLPGTGAWTGSLLAALLGMKFKKAFPAVLLGIALASVIMSIVSYG
                     LLGALIH"
BASE COUNT          524 a          453 c          515 g          486 t
ORIGIN      
        1 tgcacatcga aaaggcatat gcagatgtga gaggtgctta tccgctgatc aaccagcagt
       61 ttctggagca tgaagtatcc gggtacaaat atgtgaaccg tgaggatgac acaggagagg
      121 aaggtctgcg gactgcaaag ctgtcctatc atccggtatt tatggtggaa aaaggtattg
      181 cgaccttaaa aggagaataa gataagatga gtacatggga agagaatgtc cggaaggtga
      241 ctccctacac accgggagaa cagcccaacc ggccgcagat gatcaaactg aacaccaacg
      301 agaatcccta tccgcccgct ccaggcgtgg ctgaggtact gcgacatcca gacgaggatg
      361 cgctgcgccg ttatcctgac ccggcagcct ctgtgctggt aaatgcactg gctgagaatt
      421 atcacgtaaa gccggagcag gtgtttgtgg gagttggctc tgatgatgtg ctggctatgg
      481 cattcatgac cttctttaac tctgaaaaac cgatcctgtt cccggatatc agttattcct
      541 tctatcctgt atgggcagaa gaattccgga tcccacatac gcgggtggcg ctggatgaga
      601 atttctgcat ccgcaaggaa gattactatg gggagaatgg cggtatcgtc ttcccgaacc
      661 ccaatgctcc tacagcactg gctatgggac ttgacgatgt ggaagacatc atccgtcaca
      721 acccggatgt ggtcgtgatc gtagatgaag catacgtgga ctttggtgcg gagtctgccc
      781 tgaagctggt ggaaaaatat gagaatctgc tggtggtaca gacattttcc aaatcccgat
      841 ctctggccgg tatgcggatc ggctttgcca tcggttctcc aaagctgatc aaatatttaa
      901 acgacgtaaa atattccttc aattcctaca ccctgaacca gacctctctg gcctgcggcg
      961 tggaagctgt gaaggatcag acatatttta atgagacccg cacccgtatc atgaagaccc
     1021 gggagcatgc caaggaagtg ttaggagaga tgggattctc cttcccggac tccaaggcaa
     1081 atttcctgtt catcacccac ccggattacc cggcgaaaga actgttccag gcgctgcgcc
     1141 agaaggatat ctatgtccgt tactttgatc agccgcggat caacaattat ctccgcgtga
     1201 ccatcggaac cgatgagcag atggaagcat tttatgaatt tttgagagac tatatgaggt
     1261 aaaaaataat ggaatcaatt atgtcatggg tgatctccgc gctgagcggc catgtatccc
     1321 gggaaatgat cgttttcatc atttccatgg taccgatcct ggaactcaga ggcggtctgc
     1381 tggcagcatc tgtgctgaac atcaatatta ccagagcact gtggatctgc attatcggaa
     1441 atattattcc ggttccgttc atcctgctgc tgatcacacc gatctttaac tggcttaaac
     1501 agacgaagac cttccgtccc atggttgaga agatggaagc acgtgccttg agcaagcgtg
     1561 aacaggtaga aaaatatgaa ttctggggac tggtattgtt cgtagggatt ccgcttcccg
     1621 gaactggtgc atggaccggc tctctgctgg ctgcactgct tggcatgaaa ttcaaaaaag
     1681 catttccggc ggtgctgctg ggaatcgcac tggcatcagt gatcatgtcc atcgtgtcct
     1741 atggactgtt gggagcgttg atccactaag aactagaaac acatactggc tgggaaaccg
     1801 gcacaaagcg taaaacttgc atatgcgttc tgcgaaaagg atgaggctgt tatgggagag
     1861 aatgcaaaaa gattgttttt cgtttataat cctcactcag gaagaggaaa tatccgttca
     1921 aaattatttg acatcgttca ggtattttcc gaggcgggat atgagatcac catctatc
//
LOCUS       DBMDQJ010000474         1974 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19622_length_1974_cov_1.868660, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000474 DBMDQJ010000000
VERSION     DBMDQJ010000474.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1974)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1974)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1974
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19622_length_1974_cov_1.868660"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /gene="ruvB"
                     /locus_tag="ACZO9R_09300"
     CDS             <1..633
                     /gene="ruvB"
                     /locus_tag="ACZO9R_09300"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR0913294.1"
                     /translation="VLYPAMEDYAIDIMLGKGATARSIRLDLPKFTLVGATTRAGLLS
                     APLRDRFGVVHHLEFYTVEELKTIILHSALTLGVEIDEEGAYEMARRSRGTPRLANRL
                     LKRVRDFAQVKYDGRISKEVASFALDLLEVDKMGLDQNDRNILMTMIGKFAGGPVGLD
                     TLAAAIGEDAGTIEDVYEPYLVKNGLINRTPKGRVVTELAYQHLGIEHSV"
     gene            712..1152
                     /locus_tag="ACZO9R_09305"
     CDS             712..1152
                     /locus_tag="ACZO9R_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HIR0913295.1"
                     /translation="MAEKTSAEVIIGGKVYTLSGYEGEEYLQKVATYINNKLGEFQGM
                     EELRHLSGDMKATLLQLNIADDYFKAKTQVEKLEREIEQKEKEIYDLKHDLISNQIKA
                     ESDEKARKELEEQNKELLINKAKLEASLEDALLGKVTEKDKTEE"
     gene            1214..>1974
                     /locus_tag="ACZO9R_09310"
     CDS             1214..>1974
                     /locus_tag="ACZO9R_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HIR0913296.1"
                     /translation="MMQKRELELLAPAGSYETFEAVIRAGADAVYLGGSHFGARAYAN
                     NFNEEELLRAIDYAHIHGRQVYLTVNTLFKEEELEQGLYEYLLPYYEQGLDAVIVQDM
                     GAFARIRSMFPRMDIHTSTQMTVTGAAGAAYMKQLGASRVVMAREMSFAEIREIHDKV
                     GIEIESFVHGALCYCYSGQCLLSSMLGGRSGNRGRCAQPCRLPYEVYKKDQKSRIACG
                     NYVLSPKDLCTIDAIPALAESGIFSFKIEGRMKQA"
BASE COUNT          526 a          409 c          622 g          417 t
ORIGIN      
        1 gtgctgtacc cggcaatgga ggattatgcc attgatatca tgcttggcaa gggagccaca
       61 gcgcgctcca tccgtctgga tctgccgaag tttaccctgg tgggagcgac cacgcgcgcg
      121 ggacttttgt cggcaccgtt aagagaccgt tttggcgtgg tgcatcatct ggagttctac
      181 acggtggagg agttaaaaac gatcatcctg cattcggcat tgacacttgg ggttgagatc
      241 gatgaggaag gtgcttacga gatggcgaga cgctcgcgcg gaaccccgcg tctggcgaac
      301 cggctgttaa agcgcgtgcg cgatttcgcg caggtaaaat acgacggccg gatttcaaag
      361 gaggtagcgt cctttgcact ggatctgctg gaggttgaca agatggggct cgatcagaat
      421 gacaggaaca ttttgatgac gatgatcgga aaatttgcgg gcggaccggt cgggctggac
      481 acgctggcgg cagcgatcgg tgaggatgcc ggaaccatcg aggacgtata tgaaccgtat
      541 ctggtaaaga acgggctgat caaccgcacg ccgaagggac gggtggtgac agagctggca
      601 taccagcatc tgggaatcga acattccgta tagaaatagt tgcttttcct taaatatgtc
      661 agtataatat actatatatt gtagcggata cccgagtttg gaggaaatgg tatggcagag
      721 aagacaagcg ccgaagtgat catcggtgga aaggtataca cactgagcgg ttatgagggc
      781 gaggagtacc tacagaaggt tgccacctat atcaacaata agctgggcga gtttcagggc
      841 atggaagagt taagacatct atccggcgac atgaaggcga cgctgttgca gttaaatatc
      901 gcggatgatt actttaaggc gaagacacag gtggagaagc ttgagcgtga gatcgagcag
      961 aaggaaaagg aaatctacga tctcaaacat gatcttattt ccaatcagat caaggcagag
     1021 agcgacgaga aagccagaaa agagctcgag gagcagaaca aggagctttt aatcaacaag
     1081 gctaagctgg aggcgagtct cgaggatgcg ctgctcggca aagtaacgga aaaagataaa
     1141 acagaagaat agacaatacg gggacgtaag agatttgctt tcgtccctta ttttcatata
     1201 acaggtaaga aaaatgatgc agaaaagaga attggagctg ctggctccgg cgggatcata
     1261 cgagacgttt gaggcggtca tacgggcggg ggcggacgcg gtatatctgg gcggcagcca
     1321 ttttggagcc cgcgcatatg cgaataattt taatgaggaa gaattgctgc gtgccatcga
     1381 ctatgcacat atccatggca gacaggtgta tctgacggtg aacaccctgt tcaaggagga
     1441 ggaactggaa cagggactct atgagtatct gctgccgtat tacgagcagg ggctcgatgc
     1501 ggtgatcgtg caggatatgg gggcgtttgc aaggatacga agcatgttcc ctaggatgga
     1561 tattcacacc agcacgcaga tgactgtgac cggcgcggcg ggtgcggcgt acatgaagca
     1621 gctcggtgcg agccgggtgg tcatggcgcg ggagatgtcg tttgccgaga taagagaaat
     1681 ccatgacaag gtgggaatcg agatcgagag ttttgtgcac ggtgcactgt gttactgtta
     1741 ttccgggcaa tgtctgctca gcagtatgct gggcggcaga agcggcaacc gtgggcgctg
     1801 tgcacagccg tgccggcttc cctatgaggt ctacaaaaag gatcagaaat cccgtatcgc
     1861 gtgcggcaat tacgtgttaa gcccgaagga tctgtgtacg attgatgcca ttcccgcact
     1921 tgcagagagc ggcatattct cctttaagat tgagggacgg atgaagcagg cgga
//
LOCUS       DBMDQJ010000475         1973 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19633_length_1973_cov_1.957265, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000475 DBMDQJ010000000
VERSION     DBMDQJ010000475.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19633_length_1973_cov_1.957265"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            361..1830
                     /locus_tag="ACZO9R_09315"
     CDS             361..1830
                     /locus_tag="ACZO9R_09315"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR0913297.1"
                     /translation="MEAGSQKRAPVYEALEQLKKRRVVPFDVPGHKRGRGNPELVELL
                     GEKCVSLDVNSMKPLDNLCHPVSVIKEAEELAAEAFRADHAFFMVGGTTSSVQSMVLS
                     VCRAGDKIILPRNVHKSVINALVLCGAIPVYVNPEVDKKLGISLGMEISEVERAILEN
                     PGAVAVLVNNPTYYGICSDLRSIVKLAHEHEMLVLVDEAHGTHLYFGEDLPVCAMDAG
                     ADMASVSMHKSGGSLTQSSLLLTGKNVNWEYVSQIINLTQTTSASYLLMSSLDISRRN
                     LALRGRESFRKVAEMAEYARAEINDIGGYYAYGKDMVNGGSVYDFDVTKLSVYTRGIG
                     LAGIEVYDLLRDEYDIQIELGDIANILAYISIGDRIQDIERLVGALADVKRLYSKDPA
                     QMLNTEYINPTVLVSPQAAFYAEKKCMPIRETAGKICGEFVMCYPPGIPILAPGEMIT
                     PEIIEYIIYAKEKGCSMQGTEDPAVEHLNVLVDDRQVWK"
BASE COUNT          522 a          420 c          583 g          448 t
ORIGIN      
        1 gatatcactt gtgaaacggt caataagagt agtaatagca aatatttgct ggcgaagatg
       61 ccaacgtaga ctctgataat agtttttgca gcatgtagga cagattacat aaagtatgac
      121 tatgagaatg tgacaaggca ggtgatgtgt gcgcagcata caggcatctg tctttttttg
      181 tgtaatagga aacttcgtcc ctattacata aaaacgctcc gggcaggatt ctttcttgtg
      241 ctacagaaga tgccgtgtta tgcgaaagca tgaaagtcat gcttcctgta gagccaaaag
      301 ctctgccgga cggcatgcgc actgcggcag ggagcatcaa aacagaatgt gaggagaatc
      361 atggaagcag gatcccagaa gcgcgctccg gtctacgaag cgctggaaca attaaaaaag
      421 agaagagtcg ttcctttcga tgtgccgggg cacaagagag ggcgtggcaa cccggagctc
      481 gtggaacttc tgggggagaa atgtgtgagt ctggatgtga attccatgaa gccgcttgac
      541 aacctgtgcc atccggtgtc ggtcatcaaa gaggcggagg aactggcggc ggaggctttc
      601 cgggcagatc acgcgttttt tatggtggga ggaacaacgt cctccgtgca gagcatggtg
      661 ttgtcggtct gtcgtgccgg ggataagatc atcctgccgc gcaatgtaca taagagtgtg
      721 atcaatgcac tggtactctg cggcgctatt ccggtctatg tgaatccgga ggtggacaaa
      781 aagcttggca tttcactcgg tatggagatc tcggaggtgg agcgggcgat tctggagaat
      841 ccgggcgcgg tggcggttct tgtgaacaat ccgacttatt acggaatctg ttccgatctg
      901 cgctccatcg tgaagctggc acatgaacac gagatgcttg tgcttgtgga tgaagcgcac
      961 ggcacccatc tgtatttcgg ggaggacctt ccggtctgcg cgatggatgc cggtgcagat
     1021 atggcgtccg tctccatgca caaatccgga ggaagcttaa cacagagttc cctgctttta
     1081 accggcaaaa atgtcaactg ggagtatgtc agccagatta tcaacctcac acagacgaca
     1141 agcgcgtctt atcttctgat gtcaagtctc gacatctcgc ggagaaatct ggcgctgcgc
     1201 ggaagggaat cgttccgcaa agtggcggag atggcggaat acgccagggc ggagatcaac
     1261 gacatcggcg gatattatgc ttacggcaag gatatggtga acggcggaag cgtctatgac
     1321 tttgatgtga cgaagctctc ggtctacacg cggggaatcg gtctggcagg aatcgaggtc
     1381 tatgatctgc tgcgggatga gtatgatatc cagattgagc tgggggacat tgccaacatc
     1441 ttagcctaca tttcaatcgg cgaccgcatc caggatatcg agcggctggt gggagcgttg
     1501 gcagacgtca aacgcttgta ttccaaggat cctgcacaga tgttaaatac agaatatata
     1561 aatccgaccg ttcttgtttc accgcaggcg gctttttacg cagagaaaaa atgcatgccg
     1621 atccgggaga cggcaggaaa gatctgcgga gagtttgtga tgtgctatcc gccgggaatt
     1681 ccgattctcg cgccgggtga gatgattacg ccggagatca tcgaatatat catctatgcc
     1741 aaggagaagg gctgctccat gcagggaacg gaagatccgg cggtggagca cttaaatgtc
     1801 ttagtggatg accggcaggt gtggaaataa gaccgccgga agaatagctc ttaaggaaga
     1861 gcatacagtc aggaagaata cgaaaagaaa aaatttacgc ttttcggaag gaataactga
     1921 tatatgaagc aatataacat aaaaggaatg agttgcgccg cctgtagtgc cag
//
LOCUS       DBMDQJ010000476         1972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19648_length_1972_cov_2.024586, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000476 DBMDQJ010000000
VERSION     DBMDQJ010000476.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19648_length_1972_cov_2.024586"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="ACZO9R_09320"
     CDS             <1..795
                     /locus_tag="ACZO9R_09320"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HIR0913298.1"
                     /translation="TINIMMAASMAEGKTIIENAAKEPHVVDVANFLNSMGANIRGAG
                     TDVIRIVGVEKLHATEYSIIPDQIEAGTFMLAAAATKGDVTVKNVIPKHLEAISAKLL
                     EIGCEVEEFDDAVRVVASKPLRHTQVTTLPYPGFPTDMQPQMTVVLGVAQGTSTVTES
                     IFENRFKYVDELTRMGASIKVESNIAIISGVDQYTGARVNAPDLRAGAALVIAGLGAE
                     GITVVDDIYYIERGYEAFDQKLSLLGALIEKVSDEKEIQKFTLKVS"
     gene            967..1656
                     /locus_tag="ACZO9R_09325"
     CDS             967..1656
                     /locus_tag="ACZO9R_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6198 family protein"
                     /protein_id="HIR0913299.1"
                     /translation="MEAQEIQQKELHTVRGELALLVAVLINSFGVVLMLHSGAGISAI
                     SSVPFAFSEVFTRISLGTWTYLFQGVLVLSLMILRKRFVPQYLFSFVVGFVFGELLDV
                     HEAWINLLPLTLPNRILYFAISYLLICIGIALSNRCGLPIIPTDLFPRELADITGAAY
                     SNIKVSFDVICLAVTAGMTIFFLGHLDGLGIGTILAAFTMGKVIGVIGKWMDGRLRFV
                     SVLTPKTAKPC"
BASE COUNT          493 a          415 c          571 g          493 t
ORIGIN      
        1 accatcaata ttatgatggc ggcgtccatg gcagagggca agacaattat cgagaatgcg
       61 gcgaaggagc cgcatgtggt tgacgttgct aacttcttaa acagtatggg agccaatatc
      121 cgcggcgccg gtacggacgt gatccggatt gttggagtgg agaagctgca tgcgacagag
      181 tattcgatta ttccggatca gatcgaggca ggcaccttta tgctggcggc agcggcgacc
      241 aagggtgacg ttaccgtgaa aaatgtgatc ccgaagcatc tggaagcgat cagtgcaaag
      301 ctgcttgaga ttggatgcga ggtggaggag ttcgacgacg cggtgcgcgt ggtggcatcg
      361 aagccgctgc gtcatacaca ggtgacgaca cttccttacc cgggcttccc gacggatatg
      421 cagccgcaga tgacggttgt gctgggagtg gcacagggaa cgagtaccgt gacggagagt
      481 attttcgaga accgttttaa gtatgtggat gaactgacac gcatgggtgc aagcatcaag
      541 gtggagagca acatcgccat tatcagtggt gtggatcagt acaccggtgc cagagtcaat
      601 gcgccggatt taagagccgg agcggcgctt gtgattgccg gacttggcgc agagggcatt
      661 actgttgtgg acgatattta ttacattgag cgcggctacg aggcatttga tcagaagctg
      721 tcactgcttg gggcattgat tgagaaagtt tccgacgaaa aagaaataca gaagtttaca
      781 ctgaaagtat catagaatgg aagttttgaa tcattaggtc tgctgtggga gaaaatcccg
      841 cagcagacat tttttatttc ataggaaata ctgcggaacg ttaatacaag aaagcagaac
      901 cttcctatga aataaaaaaa cgttcttttg acatgatagc gcggaagaca gacttggagg
      961 ataacaatgg aagcacagga aatacaacag aaagagttac atactgtgag aggagaactg
     1021 gcactgcttg ttgcagtgct gattaacagc ttcggggtgg tgctgatgct gcactccggc
     1081 gcgggaatct ctgccatctc gagcgtgccg ttcgcgtttt cggaagtgtt tacgcggatc
     1141 tcgctcggca catggacata cctgttccag ggcgtcctgg tgcttagcct gatgatttta
     1201 agaaaaaggt ttgtgccgca gtatctgttc agctttgtgg tgggatttgt gttcggagaa
     1261 cttttggatg tgcatgaggc gtggatcaat cttctgccgc ttacgcttcc gaaccgtatt
     1321 ctctattttg caatcagcta tctgttgatc tgcatcggaa ttgcactgtc aaaccgctgt
     1381 ggtctgccga tcataccgac agatctgttc ccgagggaac tggcggacat tacgggcgcc
     1441 gcgtattcca acataaaggt ctcgtttgat gtgatctgtc tggcggtgac cgccggcatg
     1501 acgatcttct ttctgggaca tctcgacgga cttggaatcg gaacgattct ggcggcgttt
     1561 acgatgggaa aggtgatcgg ggtgatcgga aaatggatgg acgggcgttt gcggttcgtg
     1621 tcggttctga ctcctaaaac ggcaaagccg tgttagaaag cccccagcaa ccgggaaagt
     1681 gtacaggaga taagctgtag acaaaaaatg agggaaccat aacgttccaa tgtaaccggc
     1741 agcggcgaat tctttggagt aaaatccagg tagttcttcc gtgccgcgtc cggcatcgaa
     1801 tgctgtgtct ttccacgttt ggacagtccc cggagtgtgt ccggcaactc gcagacaatg
     1861 ggcggacttc ctagacctgt taagagatgt caacacaatt tttgattttt ttgcataaga
     1921 aataggcctg attattcagg cttttttctt atgcaactat actatattct ta
//
LOCUS       DBMDQJ010000477         1972 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19651_length_1972_cov_1.174238, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000477 DBMDQJ010000000
VERSION     DBMDQJ010000477.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19651_length_1972_cov_1.174238"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="ACZO9R_09330"
     CDS             <1..220
                     /locus_tag="ACZO9R_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HIR0913300.1"
                     /translation="VRGFIEHQGRDYLCTGYWKVYENLEQKQQLFQRFEYAKENLVLL
                     LSYGVGMADIMDIFGGTGQKTLYQECAG"
     gene            382..1164
                     /locus_tag="ACZO9R_09335"
     CDS             382..1164
                     /locus_tag="ACZO9R_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR0913301.1"
                     /translation="MNWSVFYGILIPFLGTSFGAACVFFMKKEMGDRLQRMLTGFAAG
                     MMVAASIWSLLIPAMEQVVDMGKLAFVPAVAGFWCGILFLLLLDHIIPHLHRNSQSAE
                     GPKSQLKRTTMLVLAVTLHNIPEGMAVGVVYAGYLAGSTQISAAAAMALSLGIAIQNF
                     PEGAIISMPLRAEGTGKPKAFLGGVLSGIVEPIGAILTILAAGLIVPALPYLLSFAAG
                     AMLYVVVEELIPEMSQGEHSDVGTIFFAVGFSVMMMLDVALG"
     gene            1487..1587
                     /locus_tag="ACZO9R_09340"
                     /pseudo
     CDS             1487..1587
                     /locus_tag="ACZO9R_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742718.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     assembly_gap    1588..1597
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1598..>1972
                     /locus_tag="ACZO9R_09345"
     CDS             <1598..>1972
                     /locus_tag="ACZO9R_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR0913302.1"
                     /translation="HYDGKICAAYGCSLGGSFVGLMAARGVIHMDYGILGGSDLDQSA
                     KLPAKLMTALMLPMIYPFLTTGRFRLPFLNRKLEKWRKAGDGYVDAFIGMMRGAGVDL
                     SFITKESLRNQFYSDLITPLPD"
BASE COUNT          446 a          404 c          634 g          478 t
ORIGIN      
        1 tgtgagaggg tttatcgagc atcagggaag agattatctc tgcaccgggt actggaaggt
       61 atatgagaac ctggaacaga agcagcagct attccagcgg tttgagtacg cgaaagaaaa
      121 tctggtactg ctgctttcct acggagtggg aatggcggat attatggata tcttcggcgg
      181 gaccggacaa aagacgctgt accaggaatg tgcaggttag aagtgatggg aggacactga
      241 agaagattca gtgtccgttt ttgtatcatg gaaatttttt tttgccgcac attttgcgcc
      301 ggaactggcg ggaggcttga caaataggaa gtgattgata aaataaggtt agtaaaagct
      361 aactgaaaag gaggcttgca aatgaactgg agtgtatttt acggaatatt gataccgttt
      421 cttggaacat cgttcggcgc ggcgtgcgtg ttttttatga aaaaggagat gggagaccgg
      481 ctgcagcgta tgctgacggg atttgccgca ggcatgatgg tggcggcgtc gatctggagt
      541 cttttgattc cggcgatgga gcaggttgtc gatatgggaa agctggcgtt tgtgccggca
      601 gtcgcagggt tctggtgtgg gatcctgttt cttcttctgc tcgaccatat catcccgcat
      661 ctgcaccgta acagccagag tgcggaggga ccgaagagcc agctgaagcg cacgacgatg
      721 ctcgtgcttg ccgtcacatt acataatatc ccggaaggca tggcggtcgg cgttgtgtat
      781 gcggggtatc tggcgggcag cacccagatt tccgcggcgg ccgcgatggc gctgtcgctt
      841 ggaatcgcga ttcagaattt cccggagggg gcgattattt ccatgccgct tcgggcggag
      901 ggaaccggaa agccgaaggc attcttagga ggcgtgctct cggggattgt ggagccgatc
      961 ggggcgattc tcacgatcct ggcggcggga ctgattgtcc cggcactgcc gtatctgctc
     1021 agttttgcgg cgggggcaat gctctatgtg gtcgtggagg aactgattcc ggagatgtcg
     1081 cagggagagc actcggatgt cggcacgata ttttttgcgg tgggattcag cgtcatgatg
     1141 atgctcgatg tggcgctggg ctgacacttt tattagaacg ggcggatggc tttgtgtctg
     1201 ctgggtatga aaacgccaga aaagattccg aaaaatatac catagaaaca ggagtgggaa
     1261 cagatgaaag tatatgaagt tggagagcag ggaaagccag ttctcatgct ttttccggga
     1321 accggctgtt actggcgcag caattttgga aaggtgatcg agccgctggc ggagacattc
     1381 cgggtggcgt gcgtctctta cgacgggttt gacgagacgg aacagacgca gtttcccacg
     1441 atgatcgagg agacggcgaa gatcgaggaa tatattaagg aacactatga cgggaaatct
     1501 ggctccggac ggctctgtag taaagcgttc agcagttgtc gatgagatga tggttcatga
     1561 gggccctgca agagtatttg actgcgannn nnnnnnnaac actatgacgg gaaaatctgt
     1621 gcggcgtacg gctgctcact cggcgggtca ttcgttggac ttatggcagc acgcggagtg
     1681 attcatatgg attatggcat tctcggcggt tcggatctgg atcagtctgc aaaactgccg
     1741 gcgaagctga tgacagccct gatgctgccg atgatttatc cgtttcttac gaccggcaga
     1801 tttcggcttc cgtttttaaa ccgtaagctg gagaagtggc gcaaggcggg agacggatat
     1861 gtggatgcgt ttatcggcat gatgcgcggc gccggtgtgg atctctcctt tatcacaaag
     1921 gagagcctgc gcaatcagtt ttattcggat ctgattacgc cgctgccgga tg
//
LOCUS       DBMDQJ010000478         1968 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19695_length_1968_cov_1.635779, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000478 DBMDQJ010000000
VERSION     DBMDQJ010000478.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19695_length_1968_cov_1.635779"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(9..806)
                     /gene="spo0A"
                     /locus_tag="ACZO9R_09350"
     CDS             complement(9..806)
                     /gene="spo0A"
                     /locus_tag="ACZO9R_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA];
                     GO_process: GO:0051606 - detection of stimulus [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HIR0913303.1"
                     /translation="MEKLNVAIADDNERMLRLLGDIIESDDELNVVGVARDGEEAYHV
                     IKAKEPDVVLLDIVMPKLDGLGVLDRVSQDTSIKKHPAFIMISAIGQEKITEDAFARG
                     ADYYIMKPFDNDIVLSRIKNVRRPAAGRGAEVRKVNAYEKAEETKERNLEADVTDIIH
                     EIGVPAHIKGYQYLRDAIVMSVNDMDMLNSITKILYPTIAKKYQTTSSRVERAIRHAI
                     EVAWSRGKMDTIDEMFGYTIHNGKGKPTNSEFIALITDRIRLEYKMH"
     gene            complement(904..>1968)
                     /locus_tag="ACZO9R_09355"
     CDS             complement(904..>1968)
                     /locus_tag="ACZO9R_09355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0913304.1"
                     /translation="RSWTLITYLNLAVSYYELNLYELSYKYLEQAERQLDEEVEEMYH
                     YTVLIFKCRLLWQMEKGDYVYEHMEDLLASGEKDSNASDYLSDMKDLCQLFRDMKEYE
                     YWKQTISAVETYTIDQNTVFFRLFLTELWMDYYQTVGNEERYVELCVAHADLYKRQKA
                     IDVKDRATAIDIKVQLREKEAERKRVETMSVTDALTGLGNRYSLEREAVHIIRDAGGE
                     RITVGVLDIDCFKQLNDTYGHIQGDRCLKVVADILKEAVMDCGHVYRFGGDEFVMLFP
                     AGMENRIEGIAEAILQKIAEAGIANEHSVVQPNLTISQGYACFVPEGTESGARLIEHA
                     DKALYYVKRNSRNAYYIIRE"
BASE COUNT          435 a          599 c          396 g          538 t
ORIGIN      
        1 cctcatcgtt aatgcatctt atattccaga cgtatccggt cggtaatcag tgcgataaac
       61 tccgaattcg tcggttttcc ttttccgttg tggatggtat acccgaacat ctcatcaatg
      121 gtatccatct tgcctctgct ccacgccacc tcgatcgcat gccggattgc ccgctccaca
      181 cggctggagg ttgtctggta cttcttcgcg atcgtgggat aaagaatttt cgtgatcgag
      241 ttgagcatat ccatgtcatt gacggacatg acaatcgcat cccgcagata ctgataccct
      301 ttgatatgcg ccggaactcc gatctcgtga ataatatcgg tgacatccgc ttccaggttg
      361 cgctcctttg tctcctccgc cttctcatag gcattgacct tgcgcacttc cgcaccgcgt
      421 cccgccgcag gtcttctcac attcttgatg cggcttaaca cgatatcatt gtcaaacggc
      481 ttcataatgt aataatctgc gccgcgcgca aacgcatctt ccgtgatttt ctcctgaccg
      541 atcgcgctga tcataataaa cgccggatgc ttttttatgg aggtgtcctg actcacgcgg
      601 tctaaaaccc ccaatccgtc aagcttcggc atcacaatgt cgagcagcac gacatccggt
      661 tctttcgcct ttatcacatg gtatgcttcc tcgccgtcgc gggcgactcc tacaacattc
      721 agttcgtcat cactttcgat aatatcccct aataatcgca gcattctctc attatcatct
      781 gctattgcaa cattcagttt ttccatttta gcccctcctg tgacaaaact ctttgcttac
      841 tgatgatact gattccaacg caagaaaatt tgtcgaagct agtcgtcaac tgaagtatat
      901 gcatcattcg cgtatgatat aatatgcgtt tctgctgttt ctcttcacat agtaaagcgc
      961 cttgtccgcg tgctcaatca gacgcgctcc gctctctgtg ccctccggca caaagcaggc
     1021 atacccctgg gagatggtaa gattgggctg cacaaccgag tgctcgtttg cgattcccgc
     1081 ctccgcaatc ttctgcagga tcgcctccgc gatcccctcg atccggttct ccatccctgc
     1141 cggaaacaac atcacgaact catccccgcc aaaccggtag acatgtccgc agtccatcac
     1201 tgcctccttc agaatatccg ccaccacctt caggcagcgg tctccctgaa tatgtccgta
     1261 ggtatcgttc agctgcttaa agcagtcgat gtccaaaacg ccgaccgtga tccgctcacc
     1321 gcccgcatcc cttatgatgt gcaccgcctc acgctcaaga gaatagcggt tccccagtcc
     1381 ggtcagcgca tccgtcacag acatggtctc cacgcgctta cgctccgcct ctttttcccg
     1441 cagctggacc ttgatgtcga tcgctgttgc acgatccttc acatcgattg ctttctggcg
     1501 cttatacaga tccgcatgtg ccacacacag ttccacatag cgctcttcgt tccccacagt
     1561 ctgatagtag tccatccaca attctgtcag aaaaagccgg aaaaagacgg tattctgatc
     1621 gatcgtatag gtctcgaccg cagatatggt ctgcttccag tattcatact ctttcatgtc
     1681 gcggaacaac tgacacagat ccttcatatc gctcagataa tccgatgcat tgctgtcttt
     1741 ttcaccgctc gccagaagat cctccatgtg ctcatacaca tagtctccct tttccatctg
     1801 ccagagcagc ctacatttga agatcagtac cgtataatga tacatctcct ccacttcctc
     1861 gtcaagctgc cgctctgcct gctctaaata tttgtaggaa agctcgtaca gattcagttc
     1921 gtagtaggag accgccagat tcaggtaggt aatcagagtc caggaacg
//
LOCUS       DBMDQJ010000479         1965 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19732_length_1965_cov_1.365880, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000479 DBMDQJ010000000
VERSION     DBMDQJ010000479.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19732_length_1965_cov_1.365880"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            169..603
                     /locus_tag="ACZO9R_09360"
     CDS             169..603
                     /locus_tag="ACZO9R_09360"
                     /inference="COORDINATES: protein motif:HMM:NF044614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator family protein"
                     /protein_id="HIR0913305.1"
                     /translation="MINEVLKGITDALYAAFGDDYEIHTEASMQDMEEPAFFVRCINP
                     DVPRGLTGRRKATLLFIVQYFPESDEPKKEINTVYERLSECLDLIEVEGKMVRGTIEC
                     KDISDDVMSATAEYTLFLGQSQKDAYMEECEVKGEVNSGRSS"
     gene            587..757
                     /locus_tag="ACZO9R_09365"
     CDS             587..757
                     /locus_tag="ACZO9R_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913306.1"
                     /translation="MAEAVNKVTYTKEQIIGSKKYAGRADLLSALLEPGKSYTLEEVD
                     KKMEKYMKGAVR"
     gene            757..>1965
                     /locus_tag="ACZO9R_09370"
     CDS             757..>1965
                     /locus_tag="ACZO9R_09370"
                     /inference="COORDINATES: protein motif:HMM:NF016845.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR0913307.1"
                     /translation="MYGGGQWTTQNKVLGGAYINFISAARVTTNLSERGVASMPLELD
                     WGADNVMMEVAQEDFIKNSLTLFGYAYTDDKMQPLRELFTHATKAYIYKLTSGGAKAE
                     NTYATAKCCGIRGNDLKVAIAANVDGDGFDVKLYLDTQLVDSQTVASAADLKENAWVT
                     WKETALEATAGVPLAGGTNGTVNGEVHQKYLDLLESYTVNTIGASVSDATTAKLYAAF
                     AKRMRDKVGAKFQAVLYNCAADYEGVINVKNSPDVIPWVVGLEAACGVNATCTNAIYD
                     GELEIDTAYTQTQLENAVKAGEFVLHSVGTEVRVLEDINSLVTLTEDKNELFQSNQTI
                     RVLDQIAMDIASLFNTKYHGKVQNNESGRISLWNDIASHHKQLEQLGAIENFSEDDVV
                     VSAGSEKRGVY"
BASE COUNT          551 a          391 c          585 g          438 t
ORIGIN      
        1 aaacagcgta gagtatgcca gctatattga gtttgggcat aggaaagcaa atcataaggg
       61 ctgggtgcct ggacactaca tgcttaccat gtccgagaaa gaaatccgcg atgcggcacc
      121 aggggttctt cggaaaaagc tcaataagtg gctgaatgag gtgtttgcat gatcaacgaa
      181 gttttaaagg gcatcacaga tgccttatat gccgcatttg gcgatgacta tgaaatccat
      241 acagaagcat cgatgcagga catggaagag cctgcatttt ttgtgcgctg catcaatccg
      301 gatgtgccgc gagggcttac cggccgcaga aaagccacat tgctttttat cgtgcagtat
      361 tttccggaaa gtgacgagcc aaagaaagag atcaataccg tttacgaacg tttgagcgag
      421 tgcctggacc tcatagaggt agaaggtaaa atggtgcgcg gtacgatcga atgtaaggac
      481 atatcggacg atgtgatgtc ggcaacggca gaatatacgt tattcctggg gcagagccag
      541 aaagatgcgt atatggaaga atgcgaagtg aaaggagagg taaacagtgg cagaagcagt
      601 taataaggtt acttatacca aagagcagat cattggttcc aagaaatatg cgggcagggc
      661 ggatctcctg tcggcattac tggaaccagg aaaatcttat acgcttgagg aagtggataa
      721 gaaaatggaa aaatacatga aaggagcggt gcgataatgt acggaggtgg acagtggaca
      781 acccagaata aggttttggg tggtgcttat atcaatttta tttcggcggc gcgcgtgacc
      841 acgaatctgt cagaacgtgg cgtggcatcc atgcctcttg aacttgactg gggtgcagat
      901 aacgtgatga tggaggtggc gcaggaagat ttcattaaga attcgctcac gctgttcggt
      961 tatgcctata cagatgataa gatgcagccg ttacgcgaac tgtttacgca tgcgacaaag
     1021 gcttatatct ataagctgac atcgggcggc gcaaaggcgg aaaacaccta tgcgacagcg
     1081 aagtgctgcg gaatccgcgg caatgatctg aaggttgcta ttgcggcgaa tgtggacgga
     1141 gatggctttg acgtgaagct gtatctggat acgcagcttg tggattccca gacggtagca
     1201 tccgcggcag atctgaaaga aaatgcctgg gttacatgga aagaaaccgc acttgaagca
     1261 acggcaggcg ttccgctggc aggcggtacg aatgggacgg tcaatggtga ggtgcaccag
     1321 aaatacctgg atctcttgga atcatatacc gtgaatacga tcggcgcgag tgtgagtgat
     1381 gctaccacgg cgaaactgta cgccgcattt gcaaagcgta tgagggacaa ggtcggggcg
     1441 aaattccagg cagtcctgta taactgcgcg gcggattatg agggcgtcat caatgtgaag
     1501 aacagcccgg atgtgattcc gtgggttgtg ggtctggagg cggcgtgcgg ggttaatgca
     1561 acctgtacca acgcgatcta tgacggggag cttgagattg acaccgccta tacgcagaca
     1621 cagcttgaga atgctgtgaa agccggtgaa ttcgtcctgc acagcgtggg aacggaagtg
     1681 cgtgtcttag aggacatcaa ttctcttgtg acacttacgg aggataaaaa cgaactcttc
     1741 cagagcaacc agacgatccg tgtgctggat cagatcgcga tggatatcgc atcgttgttc
     1801 aacacgaagt atcacggcaa ggttcagaac aatgaatctg gtcgtatcag cttatggaac
     1861 gatattgcat cgcatcataa gcagttagag cagcttggag caatcgagaa tttttcggag
     1921 gatgatgttg tggtctccgc cggaagcgag aagcgcggcg tatat
//
LOCUS       DBMDQJ010000480         1954 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19851_length_1954_cov_1.719374, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000480 DBMDQJ010000000
VERSION     DBMDQJ010000480.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19851_length_1954_cov_1.719374"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /gene="recJ"
                     /locus_tag="ACZO9R_09375"
     CDS             complement(<1..1663)
                     /gene="recJ"
                     /locus_tag="ACZO9R_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR0913308.1"
                     /translation="MEKWYEIKKGADFQAIAERFGISPVTARLIRNRGVIGDAAIDKY
                     LNGTCRDLYDPHLLYESEKLVCILSEKIRQGRPIRIIGDYDIDGVMSTYILYQGITRC
                     GGSVSCAIPDRMRDGYGINEHLIDAAYEEGMDTIITCDNGIAAINEIAHAKELGMTVL
                     VTDHHEIPYVEEQGVRRYLRSAADAIVNPKQQECSYPYKGLCGATVAWKIIQLLYEKF
                     CIDAEEAFAFLEFVAFATVGDVMELTDENRILVREGLKRLHRTANLGMRALILQNQLT
                     PEQITSYHIGFVLGPCVNASGRLETAKIALNLFLQKNEMDAGKIAKELVELNAQRKSM
                     TADGVELAKQIVDEGTAGEKVLVIYLPGVHESLAGIIAGRIRESYHKPVFVLTDGEEG
                     VKGSGRSTEAYSMYDEMCKCRECFSKFGGHPMAAGLSLCEDVAVFRGKINACCELTEE
                     DFIPKIRIDMAMPAAYPTIGLIRELARLEPFGNGNSKPQFADRELALLRASVVGRNQN
                     VLKLSLMTAAGEHVSAVYFGDVEAWKQYYSEKYGQTEVDAALRGRA"
     gene            complement(1670..>1954)
                     /locus_tag="ACZO9R_09380"
     CDS             complement(1670..>1954)
                     /locus_tag="ACZO9R_09380"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078955.1"
                     /note="mediates pseudouridylation (positions 38, 39, 40)
                     at the tRNA anticodon region which contributes to the
                     structural stability; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR0913309.1"
                     /translation="MKKSTVRTLYEIRVEEHESEIDLFFTGNGFLQNMVRILTGTLIE
                     VGDGRREPGSMTDVLARRDRQAAGYTAPALGLALLSVEYEGDGQNTMEQA"
BASE COUNT          406 a          660 c          408 g          480 t
ORIGIN      
        1 tcgccctgcc gcgaagcgca gcgtccacct ccgtctgccc gtatttttcg ctgtaatact
       61 gcttccatgc ctccacatcc ccaaaataga ccgcagagac atgttctccc gccgccgtca
      121 taagggacag cttcaacaca ttctggttcc tgcccaccac agaagcccgc agaagcgcaa
      181 gctccctgtc cgcgaactgt ggcttactgt tgccatttcc aaacggctct aagcgcgcca
      241 gctcccggat cagcccgatc gtcggatacg cggcaggcat tgccatatct atccttatct
      301 taggtataaa atcttcttct gtcaattcac agcaggcatt tattttgccg cggaataccg
      361 ccacatcctc gcacagagac agccctgccg ccatcggatg tccgccgaat tttgaaaaac
      421 attcccggca cttgcacatc tcgtcataca tcgagtacgc ttccgtggat ctgccggacc
      481 ccttcacccc ttcctcgccg tccgtcagaa caaataccgg cttgtggtag gactcccgga
      541 ttctccccgc aatgatcccc gccaggcttt catgcacccc gggcaaatag atcaccagca
      601 ccttctcgcc cgctgttccc tcgtcgacga tctgttttgc cagttccacg ccgtctgccg
      661 tcatgctttt gcgctgtgcg ttcagttcca cgagttcttt tgcaattttc cctgcatcca
      721 tctcattttt ttgcaagaaa agattcagtg caatcttagc ggtctcaaga cgcccgctgg
      781 cgttgacgca gggaccaagc acaaatccga tatggtacga tgtgatctgc tccggcgtca
      841 gctgattctg taaaatcaag gcgcgcatgc caaggttcgc tgtccggtgc agccgcttta
      901 atccctcccg caccagaatc cggttctcat ccgtcagttc catgacatcg cccactgtcg
      961 caaacgccac aaattccaga aacgcgaatg cctcctccgc gtcaatacaa aacttctcgt
     1021 aaagcagctg tataatcttc cacgccaccg tcgccccgca gagccctttg tacggatagg
     1081 aacactcctg ctgcttcgga ttcacaatgg catccgccgc gctccgcaga taccgccgca
     1141 ctccctgctc ctccacatag ggaatctcat ggtgatccgt caccagaacc gtcatcccca
     1201 gctctttcgc atgcgcaatc tcattgatgg ctgcaatgcc gttgtcgcag gtgatgatcg
     1261 tgtccatccc ctcctcatac gccgcatcga tcagatgctc gttgatgccg tagccgtcac
     1321 gcatgcggtc gggaatcgcg caggacacac tgccgccgca tcgtgtgatc ccctgataca
     1381 gaatataggt agacatcacg ccgtcgatgt cgtaatctcc aatgatccgg atcggtcttc
     1441 cctgccttat cttttctgac aggatacaaa ccagcttctc tgactcataa agcagatgcg
     1501 gatcatacag atccctgcat gtgccgttca gatatttatc aatcgccgca tcaccgatca
     1561 ccccgcggtt gcggatcagc cgcgctgtca cgggactgat gccaaaccgt tctgcaatcg
     1621 cctgaaaatc ggcgcctttt tttatttcat accacttttc catcttcact tatgcctgtt
     1681 ccatcgtatt ctgcccgtcg ccttcgtatt ccacggagag aagcgccagc cccagcgccg
     1741 gtgccgtgta gcccgccgcc tgtctgtccc tcctggcaag cacgtccgtc atactccccg
     1801 gctctcttct gccgtctccc acctcaatca gtgtccccgt cagaatccgc accatattct
     1861 gtaaaaaacc gttccccgtg aaaaaaagat ctatctccga ttcgtgttcc tccacacgaa
     1921 tttcataaag cgtccgcact gtggactttt tcat
//
LOCUS       DBMDQJ010000481         1947 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_19955_length_1947_cov_0.983207, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000481 DBMDQJ010000000
VERSION     DBMDQJ010000481.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19955_length_1947_cov_0.983207"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="ACZO9R_09385"
     CDS             <1..1041
                     /locus_tag="ACZO9R_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015512606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HIR0913310.1"
                     /translation="YQKMNKPQQAAYHAMQQGVLALADEIQLPRIPAEELYDVFFRLR
                     LDHPEIFWVVSYRYRYYQDSPNIIFVPEYLFEKNKIREHQKAMQSRVEKLARSAAKFT
                     EWEKEKYIHDFICENITYDKLKKPYSHEIIGPLGQGVGVCEGIAKSVKVLCDALGVWC
                     VIAICGNNPEKGIKYRHTWNIVRIGGQYYHLDATFDNTLGKHQGNAEAPGEIRYDYFN
                     LGDKAVFRDHEPLIAPAPGCPDNDHFYYKEKKLSFTKTEEVYKRAQQMAKKGRAMTFQ
                     WRGGYLTREVLQELLELIRKAGEERQKTARISLNWPQAVIHFSYVENAGIPEPEVVME
                     DANEGEQFDTGE"
     gene            complement(1063..>1947)
                     /locus_tag="ACZO9R_09390"
     CDS             complement(1063..>1947)
                     /locus_tag="ACZO9R_09390"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005602596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HIR0913311.1"
                     /translation="DKYFTPFISNRHMNSRERNDVLPEHNVGMYTVPQILTNKAEEFL
                     SLAEQLAGYGYHEVNLNLGCPSGTVVARNRGAGFLGTPRELETFLDEIFEKCPLKISI
                     KTRIGMECPDEWAPLLKIYQKFPMKELIIHPRLQKEGYKGTPHLEAFAEAVEALQCPL
                     CYNGDITSPESLTQILTKLPSIDTVMIGRGILQNPGLLQELKAASTESVALPSCEERL
                     AVLKEFHTSLLTGYQEIMSGDTNTLYKMKDLWTFLSHSFESPDKYVKKIRKATDTSDY
                     KLAVNALFRECALKSENS"
BASE COUNT          509 a          461 c          524 g          453 t
ORIGIN      
        1 tatcagaaaa tgaacaagcc ccagcaggcc gcctatcacg ctatgcagca gggagtgctg
       61 gcgctggcag acgagatcca gctgccgcgg atcccggcgg aagaattgta tgacgtattt
      121 ttccgactgc ggctggatca tccggagatc ttctgggtgg tgagttatcg gtatagatat
      181 tatcaggact cccccaacat catcttcgtg ccggagtatc tgtttgagaa aaataagatc
      241 agagaacatc agaaagccat gcagtcccgg gtggaaaagc tggcaagatc tgccgcaaag
      301 ttcacagagt gggaaaaaga gaagtacatt cacgacttta tctgcgaaaa tatcacctac
      361 gataagttga aaaagcccta ttcccatgag attatcggac cgctgggaca gggcgtgggt
      421 gtctgcgagg gcattgccaa atcggtgaag gtgctttgtg atgcgctggg tgtgtggtgt
      481 gtaatcgcta tctgcggcaa caatccggag aaagggatca agtaccgtca cacttggaat
      541 attgtgcgga tcggcgggca gtattaccat ctggatgcca catttgacaa tacattggga
      601 aaacatcagg gcaatgcgga agcacccgga gagatccgct acgactattt caatctggga
      661 gataaagcag tgttccggga tcatgaaccg ttgatcgccc cggcacccgg ctgtccggac
      721 aacgatcatt tttattacaa ggagaaaaaa ctttccttta cgaaaacgga agaagtatat
      781 aaaagagcac agcagatggc gaaaaaaggc cgggccatga cgttccagtg gcggggaggc
      841 tatctgacca gagaagtctt gcaggaactg ttggaactga tccgcaaggc aggcgaggag
      901 cggcagaaga ccgcccggat cagtctcaac tggccacagg cggtcataca tttcagctat
      961 gtggaaaatg caggcatccc ggagccggag gtcgtcatgg aagacgccaa cgaaggagaa
     1021 cagtttgaca ccggggaata aaggtatggg atgatgtcag gatcaagaat tctccgactt
     1081 caatgcgcac tcccggaata acgcattgac ggccagctta tagtcgctgg tatctgtggc
     1141 tttgcggatc ttttttacgt atttgtccgg agattcgaag ctgtggctta agaaagtcca
     1201 gagatccttc atcttataga gcgtattggt atcgccggac atgatctcct gataaccggt
     1261 caggagggaa gtatggaatt ccttcaggac agccagacgc tcttcacaag acggcagcgc
     1321 aacactctcc gtggaggcag ccttcaattc ctgcaataat cccggattct gcaaaattcc
     1381 ccggccgatc atgaccgtat cgatactggg aagcttcgta agaatctgcg ttagcgactc
     1441 cggagaggta atatccccgt tatagcacag cggacattgc agggcttcca ccgcctctgc
     1501 gaaagcttcc agatggggtg tccccttgta accttccttt tgcagtctgg ggtgaatgat
     1561 cagctccttc atgggaaatt tttgatatat tttcagtaac ggagcccact catcaggaca
     1621 ttccatgccg atgcgggttt taatggaaat tttcaacggg catttttcaa aaatttcgtc
     1681 cagaaaagtc tccagttccc ggggcgtacc taagaatccg gcaccgcggt ttcttgctac
     1741 cactgttcca gaagggcagc ccagattcag attgacctcg tggtagccat atccggccag
     1801 ctgctctgcc agcgacagaa attcctccgc cttattggtc aggatctgcg gtacggtgta
     1861 catgcccaca ttatgttccg gaagcacatc gttgcgttct ctgctgttca tgtgccgatt
     1921 ggagataaaa ggggtaaaat atttgtc
//
LOCUS       DBMDQJ010000482         1943 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20001_length_1943_cov_1.047774, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000482 DBMDQJ010000000
VERSION     DBMDQJ010000482.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20001_length_1943_cov_1.047774"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(108..1895)
                     /gene="argS"
                     /locus_tag="ACZO9R_09395"
     CDS             complement(108..1895)
                     /gene="argS"
                     /locus_tag="ACZO9R_09395"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR0913312.1"
                     /translation="MKKILDLITGEVTQAFVDCGYDAKYGKVTLSNRPDLCEYQCNGA
                     MAAAKEYKKAPFMIADEVAEKLSGNAMFSMVESVKPGFLNLKLDEAYLADYVAKMQAD
                     EGRYGCEKTKEPKTIMIDYGGPNVAKPLHVGHLRSAIIGESIKRIGRFMGHTVIGDVH
                     LGDWGLQMGLIITELRERKPDLVYFDESYTGEYPAEAPFTISELEEIYPTASGKSKED
                     AVYKEAAMQATYELQHGRRGYQALLSHILNVSVTDLKRNYENLNVSFDLWKGESDAQP
                     YIPDMVQKMKDDGFAYISDGALVVDVKEDTDTKEIPPCMILKSDGASLYNTTDLATIV
                     WRMKDYHPDELIYVVDKRQELYFTQVFRCARKTGLVKPETGLKFLGFGTMNGKDGKPF
                     KTREGGVMRLQYLLESVNEEMLKKITENQKAKENLEISEEEAKETAKMIALAAIKYGD
                     LSNQASKDYIFDIDRFTSFEGNTGPYILYTIVRIKSILNKYHSMGKDESGAVIEAAHS
                     ASEKNLMLALSGFGAMMENAYEETAPHKICSYIYELANAFNSFYHETKIMSEENEQIQ
                     KSYIRLLELTKSVLETCIDLLGFKAPERM"
BASE COUNT          417 a          574 c          422 g          530 t
ORIGIN      
        1 gcgtgttcgg gactttcacc cgttagagcg cgcccatggc gcgcaaacca aaaggggcag
       61 cccgtttcat gcggcagccc catctttttt atcaactcac taaatggtta catgcgctcc
      121 ggcgccttaa agccaagcag atcgatgcag gtctcaagca ccgacttcgt cagctctaac
      181 agacggatat aagacttctg gatctgctcg ttctcctcgg acatgatctt tgtctcatgg
      241 taaaagctgt tgaatgcatt cgccagctca taaatgtaag aacagatctt atgcggcgct
      301 gtctcctcat aagcgttctc catcatggca ccaaatccag aaagtgccag cataaggttc
      361 ttctcgcttg cggaatgagc cgcctcaatc actgcgccgc tctcatcctt ccccatgctg
      421 tgatacttgt tcaaaatcga cttgatacga acgatggtgt aaaggatgta tggtccggta
      481 tttccctcaa acgaggtgaa ccggtcaata tcaaagatgt aatccttgct tgcctggttg
      541 gagaggtcgc cgtacttgat ggctgcaagc gcgatcatct tcgcggtctc cttcgcctct
      601 tcctcactga tctcaaggtt ctcttttgcc ttctggttct ccgtgatctt tttgagcatt
      661 tcctcgttca cactctcaag cagatactgc agacgcataa cgccgccctc acgggtctta
      721 aacggcttgc catccttgcc gttcatcgtg ccgaatccga ggaactttaa gcctgtctct
      781 ggctttacca gtccggtctt acgcgcgcag cggaacacct gtgtaaaata gagttcctgg
      841 cgcttatcca ccacatagat gagctcatcc ggatggtaat ccttcatacg ccacacgatc
      901 gttgcgagat ccgtcgtatt ataaagggat gcaccgtctg attttaagat catgcacggc
      961 gggatctcct tggtatcggt atcctccttg acatccacga caagcgcacc gtcgctgatg
     1021 taggcaaatc cgtcatcctt catcttctgc accatatccg gaatgtacgg ctgggcatcg
     1081 gactcgccct tccacagatc aaacgataca ttcagattct catagttgcg ctttaagtcc
     1141 gtgacggaca catttaagat gtgggaaagc agtgcctgat aacctcttct gccgtgctgc
     1201 agttcatagg ttgcctgcat cgccgcctcc ttgtagactg cgtcctcctt ggacttgccg
     1261 ctggctgtcg ggtagatctc ctcaagctct gagatggtaa acggcgcctc tgccggatac
     1321 tcgccggtat aactctcatc aaaatatacc agatccggct ttctctcccg aagctccgtg
     1381 atgataagtc ccatctgtaa gccccagtcg cccagatgca catcgccgat taccgtatgt
     1441 cccataaaac gtccgatgcg cttgatgctc tcgccgatga tcgccgagcg cagatgtccg
     1501 acgtgaagcg gcttcgccac gttcggtccg ccgtaatcga tcatgatcgt cttcggctct
     1561 ttggtcttct cacagccgta tcttccctcg tccgcctgca tctttgccac ataatccgcc
     1621 agatatgcct catccagctt gagattcaaa aatcccggct ttacagattc caccatggaa
     1681 aacatggcat tcccggaaag cttctccgcc acttcgtccg cgatcataaa cggcgccttc
     1741 ttgtactcct ttgccgccgc catcgctccg ttgcactgat attcacagag atccggtcta
     1801 ttggataatg tcaccttgcc atactttgca tcgtaaccgc agtccacaaa tgcctgtgtg
     1861 acttccccgg tgataaggtc taagatcttt ttcatattct tctctcctgc atttcgttca
     1921 aactgtttct ttacaacctg tat
//
LOCUS       DBMDQJ010000483         1940 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20036_length_1940_cov_1.533986, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000483 DBMDQJ010000000
VERSION     DBMDQJ010000483.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20036_length_1940_cov_1.533986"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /gene="greA"
                     /locus_tag="ACZO9R_09400"
     CDS             complement(<1..324)
                     /gene="greA"
                     /locus_tag="ACZO9R_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR0913313.1"
                     /translation="MDKKNILTYEGLKKLEDELEDLKVVKRKEVSQKIKEAREQGDLS
                     ENAEYDAAKDEQRDIEARIEELEKILKNAEVFVEEEADLDKVSIGCQVKILDCEFDEE
                     LTYKIV"
     gene            complement(358..906)
                     /locus_tag="ACZO9R_09405"
     CDS             complement(358..906)
                     /locus_tag="ACZO9R_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913314.1"
                     /translation="MSYSNEFLRNFRPVFLAILKAQSFRPERVELVVIDEERSHVFEP
                     ADVRDVLEQIGDDLNYLTVYTGRPAYFSAFAERMYEENGLVVMLFPKNGLSEGKGQAG
                     VRETFLTRKVILDFEWEGAYRTEMICAAATYIPIHKKPWKQGENLDIMVPIGYNTVIV
                     KSMHHQREKLGRDRFEEAFYEN"
     gene            complement(910..1911)
                     /gene="dusB"
                     /locus_tag="ACZO9R_09410"
     CDS             complement(910..1911)
                     /gene="dusB"
                     /locus_tag="ACZO9R_09410"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007883424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR0913315.1"
                     /translation="MAIIRTLQIGNVTLENNLILAPMAGVSDLPFRLLCREQGAGLVC
                     MEMVSAKAILYKNRNTEELLTIDPKEHPVSLQLFGSDPDIISEIAKQIEERPFDILDL
                     NMGCPVPKVVNNGDGSALMKNPRLAGEIIEKTARAIKKPLTVKIRKGFDDAHVNAVEL
                     AHIAQESGAAAVAVHGRTREQYYAGHADWDIIRQVKEAVSIPVIGNGDIRTPEDVAAM
                     AEQTGCDGYMIARGAEGNPWIFRQILHYFETGEHLARPDFSEVTEMLLRHAKMQIDCK
                     GDYTGIREIRKHAAWYTAGYRNSSKLRGRINEVENYEQLEALFREVESYNEKNGYEA"
BASE COUNT          396 a          604 c          396 g          544 t
ORIGIN      
        1 cacgatcttg taggtcagct cctcgtcaaa ttcacaatct aagatcttta cctgacatcc
       61 gatgcttacc ttatctaagt cagcctcttc ctctacaaat acctcagcgt tcttgaggat
      121 cttctcgagc tcctcaatac gtgcctcaat atctctctgc tcgtccttcg ctgcatcata
      181 ctctgcattc tcggacagat cgccctgctc tcttgcttcc ttgatcttct gggagacttc
      241 ctttctcttg acaaccttta aatcctctaa ttcgtcctcc agttttttga gtccttcata
      301 cgtcagaatg tttttcttgt ccattactct ctacccttct ttgtgatttt tatcagttca
      361 attctcataa aaagcttctt caaatcggtc acgtccaagc ttttctctct ggtgatgcat
      421 gctcttaaca atcacagtat tataaccgat aggcaccatg atgtcaagat tttcgccctg
      481 tttccagggc tttttgtgga tcggaatgta ggttgccgcg gcgcagatca tctctgtccg
      541 gtatgctccc tcccactcaa aatccaggat taccttccgg gtcagaaacg tttctctcac
      601 ccccgcctgt cctttcccct cggacagtcc gttttttgga aaaagcatca ccaccagccc
      661 gttctcctca tacattctct ctgcaaacgc ggaaaaatat gcggggcgtc cggtgtacac
      721 cgtaagataa ttcagatcgt ctccgatctg ttcgagcaca tcgcgcacat ccgccggttc
      781 aaagacatgg ctccgctcct cgtcaatcac cacaagctcc acgcgctccg ggcgaaaact
      841 ttgcgccttt aagatcgcca gaaataccgg tcggaaattt ctaagaaact cattggaata
      901 ggacatttcc tatgcctcgt atccgttttt ctcattatat gactccactt cccggaataa
      961 tgcctcgagc tgctcgtaat tctccacctc gttgatcctt ccgcgaagct tggacgaatt
     1021 acggtatccc gcggtgtacc atgccgcatg cttgcggatc tcacggatgc ccgtgtagtc
     1081 tcccttgcag tcgatctgca tctttgcatg gcgcagcagc atctccgtca cctctgagaa
     1141 atccggccgg gcaaggtgct cgcccgtctc aaaataatgc agaatctgac ggaaaatcca
     1201 gggatttccc tccgctccgc gcgcgatcat gtagccgtcg cagccggtct gctctgccat
     1261 cgccgccaca tcctccggcg tgcggatatc cccgtttcca atgacgggaa ttgataccgc
     1321 ctccttcacc tggcggataa tgtcccagtc cgcatgtccg gcataatact gctctctggt
     1381 gcgcccatgc accgcgaccg ccgccgcacc gctctcctgt gcaatgtgcg ccagctcgac
     1441 cgcattgacg tgtgcatcgt cgaatccctt gcggatcttc accgtcagcg gttttttgat
     1501 cgcccgcgcc gtcttctcaa tgatctctcc ggcaagccgc ggatttttca tgagcgcgga
     1561 gccgtccccg ttgttgacga ccttcggaac cggacagccc atattcagat ccagaatgtc
     1621 aaacggccgt tcctcaatct gcttcgcaat ctcactgata atatccggat cggagccgaa
     1681 aagctgcagc gataccgggt gctcctttgg atcaatggta agaagctcct ccgtgttgcg
     1741 gttcttatac agaatcgcct tggcgctcac catttccatg cacaccagcc ccgcgccctg
     1801 ttccctgcaa agcaagcgaa atggcaggtc gcttacgcct gccattggtg ccagtatcaa
     1861 attattttct aaggttacat ttcctatctg cagtgttcgg attattgcca cgctgccacc
     1921 ctcattttct acttatttaa
//
LOCUS       DBMDQJ010000484         1939 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20063_length_1939_cov_0.986942, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000484 DBMDQJ010000000
VERSION     DBMDQJ010000484.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20063_length_1939_cov_0.986942"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="ACZO9R_09415"
     CDS             complement(<1..757)
                     /locus_tag="ACZO9R_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR0913316.1"
                     /translation="MGNKKRKNTTSFLAQGTILAAASLISRVIGLIYRMPLTAIIGDT
                     GNDYYGSAYTIYNILLIISSYSLPLAVSKLVSANISQGRRRNVYRILKCALIFALVSG
                     TAAAAILYFGADFITGTLLRTPLCIFALKVLVPVLVIVAVLGVMRGFFQGLGTMMPSA
                     VSQILEQIVNAIVSVWAAYVLFSYGSKAGALLGNAEDYGAAYGAAGGTIGTAAGALSA
                     LLFAGFVLVVYLRVFKKTLRKERKTSADSYGEIF"
     gene            complement(804..1304)
                     /gene="ybeY"
                     /locus_tag="ACZO9R_09420"
     CDS             complement(804..1304)
                     /gene="ybeY"
                     /locus_tag="ACZO9R_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR0913317.1"
                     /translation="MSLNLEVEVDVPFTFDYEALAGEVVAFTIDHEGFPYEAEVNLTL
                     TDNEGIHEINRMYRQIDAPTDVLSFPMLSYEQAGDFSALEEDYEDNFNPDTGEIMLGD
                     IVISVDRVREQAAAYGHSERREFAFLIVHSMLHLFGYDHMTPEDAAVMEPKQRQILEE
                     MNISRD"
     gene            complement(1301..>1939)
                     /locus_tag="ACZO9R_09425"
     CDS             complement(1301..>1939)
                     /locus_tag="ACZO9R_09425"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR0913318.1"
                     /translation="LTYREISVRIMVSAVADGVVVFLFLHLCYRKLADARNAEVDEVM
                     VDITDESYPLARELASFSRADYSHARRVMLVAARCAAVVGADERVCSAAGMYYRIGVL
                     EGAPLAKNGVKMAQRACFPEKVIRIIGEYNGEEALPSTIESAIVHMVDGLLKKLEVLD
                     EDTVGSNWNQDMVIYQTLNEYSAAGLYDRSGLSMNMFLKIREYLVNEEALLV"
BASE COUNT          486 a          597 c          424 g          432 t
ORIGIN      
        1 taaaaatctc tccgtacgaa tctgcgcttg tctttcgctc cttgcgaagc gtctttttaa
       61 acacccgcag atagacaaca aggacaaaac ccgcaaacag cagcgcactc agcgctccgg
      121 cagcggttcc gatcgttccg cccgccgcac cgtaggctgc gccgtaatcc tcggcattgc
      181 ctaaaagcgc tccggctttg gaaccgtagg aaaaaaggac atacgccgcc cacacgctga
      241 caatcgcatt tacaatctgc tctaagatct gggatacggc actcggcatc atcgtgccaa
      301 gtccctggaa aaagcctcgc atcacaccta agaccgcaac aattacaaga accggcacta
      361 aaacctttaa cgcaaagata caaagtgggg ttctaagcag cgtacccgta ataaaatccg
      421 ctccgaaata gagaattgcc gcggcagcgg ttccggacac cagcgcaaaa atcagcgcgc
      481 actttaagat gcggtagaca ttgcgtcttc tgccctgtga gatatttgcc gagacaagtt
      541 tggataccgc gagcggcagg ctgtaggacg aaataattaa caaaatatta tagatggtat
      601 aggcactgcc ataatagtcg ttgccggtat ccccaataat cgccgtcagc ggcatgcggt
      661 aaatcagtcc gatcacacgg ctgatcagcg aagccgccgc gaggattgtg ccctgcgcca
      721 gaaaacttgt cgtatttttc ctttttttat tgcccataaa agtacctcaa atcctttcgg
      781 aagcgccacg gcgccgccgt tcgttaatca cgcgagatgt tcatctcctc cagaatctgt
      841 ctctgcttcg gctccatgac tgccgcgtcc tccggcgtca tgtggtcata cccgaacaga
      901 tgaagcatgc tgtgcacgat cagaaacgca aattccctgc gctcgctgtg tccgtaggcg
      961 gcagcctgct ctctcacccg gtccaccgag atgacaatgt ctcccagcat gatctccccg
     1021 gtatccggat taaaattatc ctcataatcc tcttccaacg ccgagaaatc gcccgcctgc
     1081 tcataggaga gcatcgggaa agacaaaacg tccgtcggcg catcaatctg ccggtacatc
     1141 cggttgatct catgaattcc ttcattgtcc gtcagcgtca gattcacttc cgcctcataa
     1201 ggaaaacctt catggtcgat ggtaaaagcg acaacctcgc ccgccagcgc ttcataatca
     1261 aatgtaaatg gcacatccac ttcgacttct aaattcaggc tcataccagt aatgcctcct
     1321 cgttcacaag atattcccgt attttcaaaa acatattcat gctaagcccg gaacggtcat
     1381 acagccccgc ggcagaatat tcattcaagg tctgatagat caccatatcc tggttccagt
     1441 tgctgccaac ggtatcctca tccagaacct ccagtttttt caacagcccg tccaccatat
     1501 gtacaatcgc cgactcgatg gtggatggca gggcttcttc cccgttgtat tccccgataa
     1561 tgcggattac cttctccggg aagcaggctc tctgtgccat tttcacaccg ttttttgcaa
     1621 gcggtgcgcc ctccagtacg ccgatacggt aatacatccc cgcggcgctg cacacacgtt
     1681 cgtctgcccc gaccacagca gcacatcgcg ccgccacaag catcacccgc cttgcatggc
     1741 tgtagtctgc cctggaaaaa ctcgccagct cccgcgccag cggataggat tcatcggtga
     1801 tgtcgaccat gacctcatcg acttccgcat ttcttgcatc cgccagcttc cggtagcaca
     1861 gatgcaaaaa caaaaagacg accacaccat ccgccacggc agataccatg atccggacgc
     1921 tgatctcccg gtaggtcag
//
LOCUS       DBMDQJ010000485         1933 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20133_length_1933_cov_1.566594, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000485 DBMDQJ010000000
VERSION     DBMDQJ010000485.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1933)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1933
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20133_length_1933_cov_1.566594"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(17..87)
                     /locus_tag="ACZO9R_09430"
     tRNA            complement(17..87)
                     /locus_tag="ACZO9R_09430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53..55),aa:Gly,seq:tcc)
     gene            complement(94..168)
                     /locus_tag="ACZO9R_09435"
     tRNA            complement(94..168)
                     /locus_tag="ACZO9R_09435"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(131..133),aa:Pro,seq:tgg)
     gene            complement(268..747)
                     /locus_tag="ACZO9R_09440"
     CDS             complement(268..747)
                     /locus_tag="ACZO9R_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR0913319.1"
                     /translation="MKILVVSDTHRKLENLKVILEREQPMDLLIHLGDAEGDEDSIAE
                     MAGCRLEIVAGNNDFFSNLPREKELRIGSYRVLIMHGHYYYVSAGIADIEREAAAQGY
                     DIVMFGHTHRPVIDYTRDVIALNPGSLSYPRQEGRRPSYIVMDLDKNGKANFEIKYL"
     gene            complement(751..1344)
                     /locus_tag="ACZO9R_09445"
     CDS             complement(751..1344)
                     /locus_tag="ACZO9R_09445"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HIR0913320.1"
                     /translation="MRIIFATGNAGKMREIREIMADMDMEICSMKEAGISLDIEENGT
                     TFEENAQIKAKAVAACTKDIVLADDSGLEVDYLNKEPGVYSARYLGEDTSYDIKNQAI
                     LDRLKGVPKEQRTARFVCAIAAVLPDGKTLVTRETIEGYIGECPAGENGFGYDPIFYV
                     DEYHCSTAELTEEQKNEISHRGKALRSMKKLLHEYGF"
     gene            complement(1360..>1933)
                     /locus_tag="ACZO9R_09450"
     CDS             complement(1360..>1933)
                     /locus_tag="ACZO9R_09450"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR0913321.1"
                     /translation="IAAELIDFLHEFLGHGGRAVGANKQGINEKDIDLAIVLKLKEIF
                     DNSDQNIGVYYTRTDDSNPTFDQRVQLANKSDADLFISIHNNSTRSGRMSGTSGTQVM
                     YDETSEASRQFAQICLEEVTGRIGSRDKGLVEGDSIYIIRTSEVPVALIEVGFMTNKD
                     ELEKLNSDAYQRETAEGVYQAVLRAFEEGY"
BASE COUNT          428 a          586 c          391 g          528 t
ORIGIN      
        1 gtcagtgcag atttaaagcg ggtgatggga atcgaaccca cgtatccagc ttggaaggct
       61 ggtgttctac cattgaacta cacccgcacg aaatcggggt gacaggattc gaacctgcga
      121 cctcctggtc ccaaaccagg cgctctagcc aagctgagcc acaccccgaa ggattttatc
      181 gcttgcgcac tcttccggcc actgagctgt ccttttcgtg acgcaaggat tattatatag
      241 gacagcttct gaaaagtcaa cactttttta taaatatttt atttcaaaat ttgcctttcc
      301 gtttttgtcc agatccatca caatgtagga cggtcttctc ccctcctgcc tcggatagga
      361 aagactgccc ggattgagcg caatcacatc ccttgtgtaa tcgatcacag gcctgtgcgt
      421 gtgtccgaac atcacaatat catagccctg tgccgccgct tcccgctcaa tatcggcgat
      481 ccccgcactg acatagtaat aatgtccatg catgatcaat acacggtagc tgccgatgcg
      541 aagttctttt tcccgtggaa gattggaaaa aaagtcattg ttgcccgcaa cgatctccag
      601 ccggcagccc gccatctccg caatactgtc ctcatctccc tccgcgtcac ccagatggat
      661 cagaagatcc atcggctgct cacgctctaa gataactttt aaattttcaa gtttcctgtg
      721 cgtatcgctt acaaccagta ttttcatagg ctaaaacccg tactcatgca gcagcttctt
      781 catggaacga agtgcttttc ctctgtggct gatctcattc ttctgctctt ctgtcagctc
      841 cgccgtcgag cagtgatact catccacata gaagatcgga tcatatccaa atccattctc
      901 ccctgccgga cattccccga tatatccctc gatcgtctcc cgtgtcacaa gtgtcttccc
      961 gtccggaagc actgctgcaa tcgcacagac aaaccgcgcc gtgcgctgct ccttcggcac
     1021 gcccttcaaa cggtcgagaa tcgcctgatt cttaatatca taggaagtat cctcgcccag
     1081 ataccgcgcc gaatatacgc ccggctcctt gtttaaataa tccacctcca gaccggaatc
     1141 atcggccagc acaatatcct tcgtacaggc agctactgcc ttcgccttga tctgcgcatt
     1201 ctcctcgaac gtcgtgccgt tctcctcaat atccagggaa atacctgctt ccttcatgga
     1261 acagatctcc atatccatat ccgccataat ctcgcggatt tctctcattt ttccggcatt
     1321 gccggtggca aaaattattc tcatggcttt gtcctctttt tagtatcctt cttcaaatgc
     1381 gcgaagcact gcctgataga caccctccgc cgtctccctc tgatatgcat cggaattcag
     1441 cttttccagt tcatctttgt tcgtcataaa tccgacttca atcagcgcaa ccggcacctc
     1501 actggttcta atgatataaa tactgtcgcc ttccaccagt cccttgtcac ggcttccgat
     1561 cctgcctgtg acttcctcca gacagatctg cgcgaactgg cggctcgcct ccgacgtctc
     1621 gtcgtacatg acctgcgtac cgctggtacc cgacatgcgt ccgcttctcg tcgagttgtt
     1681 atgtatactg ataaaaagat ccgcgtccga cttgttggca agctgcactc tctggtcgaa
     1741 tgtcggattg ctgtcatccg tcctggtgta atacacgccg atgttctgat cgctgttgtc
     1801 aaaaatctct ttcagcttca gcacgatcgc caggtcaatg tccttctcat tgattccctg
     1861 cttgttcgca ccgacggctc ttcctccgtg ccccaggaat tcgtggagaa aatcaataag
     1921 ttcggcggca ata
//
LOCUS       DBMDQJ010000486         1932 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20144_length_1932_cov_1.379028, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000486 DBMDQJ010000000
VERSION     DBMDQJ010000486.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20144_length_1932_cov_1.379028"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="flhF"
                     /locus_tag="ACZO9R_09455"
     CDS             complement(<1..1028)
                     /gene="flhF"
                     /locus_tag="ACZO9R_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HIR0913322.1"
                     /translation="MTINKYQGKTKEEAVEKAKQELGAGAVVLNVKEIKPKGMFKMFK
                     NTLYEVTAAVEEKEAFPAQTLRSETKPVHDTISLAADEKITIPGVARENTRPAVAPAP
                     AEQEVVPMKEAKRESTNEGLEKRLENLSNILEKQFSVEEMRKSHPVEPQEKENRTEGF
                     QFIKMLYRTLLENDVNEKYVNQIMDEAEKVLHNGSSVDSILSNVYQKLILKFDQPQTI
                     ELTGKKPKVIFFIGPTGVGKTTTLAKIASRYKVDKEKKVAFLTADTYRIAATEQLRIY
                     ANILDAPMSIIYTPEEMNDAIARVSEYDLVFVDTAGFSHRNEAQCQDMKKLIDGLDEA
                     YEKEVYLVL"
     gene            complement(1029..>1932)
                     /locus_tag="ACZO9R_09460"
     CDS             complement(1029..>1932)
                     /locus_tag="ACZO9R_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
                     /protein_id="HIR0913323.1"
                     /translation="ELGTVVPIIRLRDNIQLNPNQYIIKIKGIQVTEGEILFDHYMAM
                     NPGYVEEEITGIPTFEPSFHLPAIWITESQRESAESLGYTVVDPPSIIATHLTEVIRS
                     HIAELLTRQDVQNLVNNLKESNPVLVDELVPKLLGLGEIQKVLQNLLSEGISIRDLLT
                     IFETLADHAPATRDTDVLTEYARQSLKRAISSKYFPANETTSVITVDPKIEQEIMGSV
                     KQTEQGAYLTLDPERTKAIMASVEEEVAKLENMGKPAIIMTSPIVRMYFKKLTEDYFK
                     DLTVLSYNEVESNVELQSVGMVTI"
BASE COUNT          393 a          579 c          420 g          540 t
ORIGIN      
        1 agcacgaggt atacctcttt ctcgtaagcc tcatccagtc cgtcgatcag ctttttcata
       61 tcctggcact gtgcctcatt gcggtgcgag aatcccgccg tgtcaacaaa taccagatca
      121 tattcgctta cgcgcgcaat ggcatcgttc atctcctccg gcgtatagat aatgctcatc
      181 ggagcatcca gaatatttgc gtagatgcgc agctgctctg tcgcagcaat ccggtaggtg
      241 tccgcagtca gaaatgccac tttcttctct ttgtctacct tatagcgtga cgcaatcttg
      301 gcaagcgttg ttgtctttcc cacgccggtc ggtccgatga aaaaaatcac cttcggcttt
      361 ttgcctgtaa gctcaatcgt ctgcggctga tcgaacttta agatcagctt ctgataaacg
      421 ttggaaagaa tagaatccac actgcttccg ttgtgaagca ccttctccgc ctcgtccatg
      481 atctgattga catacttctc attcacatca ttctccagca gcgtgcggta gagcatcttg
      541 ataaactgga acccttccgt gcggttctcc ttttcctgcg gctctaccgg atgagacttg
      601 cgcatctcct ccacggaaaa ctgcttttcc agaatattcg acaggttctc cagccgcttt
      661 tccagtccct cattcgtaga ttcgcgcttt gcttctttca taggaacaac ctcctgttcc
      721 gccggtgccg gtgccactgc cggtctggtg ttctctcttg ccacccccgg aatggtaatc
      781 ttttcatctg cagccagact gatcgtatcg tgcacaggct ttgtctcgct gcggagcgtc
      841 tgcgccggga acgcttcctt ttcctccacc gcagccgtca cctcatacag cgtgttctta
      901 aacatcttaa acatgccctt gggcttaatc tccttcacgt tcagcacgac cgcccccgcg
      961 cccagctcct gctttgcctt ctcgacggcc tcttcctttg tcttgccctg atatttgtta
     1021 atggtcattt atattgtcac catccctact gattgtaatt ctacgttgga ttccacctca
     1081 ttgtaagaca gcaccgtcag atccttgaag taatcctcgg tcagcttctt aaagtacata
     1141 cgcacaatcg gcgatgtcat aatgatggca ggcttgccca tgttctccaa cttcgccact
     1201 tcctcctcaa cggatgccat aatcgccttc gtgcgctccg gatcgagcgt cagatatgcc
     1261 ccctgctccg tctgcttgac agaacccatg atctcctgct cgatcttcgg atctaccgtg
     1321 atcacgctgg ttgtctcatt cgccgggaaa tatttgctgg agatcgcgcg ctttaagctc
     1381 tggcgtgcgt actccgtcaa cacatcggta tccctggtcg ccggcgcatg gtctgccagc
     1441 gtctcaaaaa ttgtgagcag atctcggatg gaaattccct ccgagagcag attctgcaga
     1501 accttctgga tctcgccaag tccaagaagc ttcggcacca gttcatccac gagaaccgga
     1561 ttgctctcct tgaggttatt gacaaggttc tgtacatcct gtctggtaag cagctctgcg
     1621 atatgggaac ggatcacctc ggtcagatgc gtcgcaatga ttgacggcgg atccaccacc
     1681 gtatagccaa ggctctccgc actctctctc tggctctccg tgatccagat tgccggcagg
     1741 tggaaggacg gttcaaacgt cggaattccc gtaatctcct cctccacata tcccggattc
     1801 atcgccatat agtgatcgaa caggatctct ccctcggtca cctgaattcc tttgatcttg
     1861 ataatgtact ggttcggatt cagctgaatg ttatcacgca gtcggataat cggcacaacc
     1921 gtaccaagtt cc
//
LOCUS       DBMDQJ010000487         1928 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20187_length_1928_cov_1.866995, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000487 DBMDQJ010000000
VERSION     DBMDQJ010000487.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20187_length_1928_cov_1.866995"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(240..1844)
                     /locus_tag="ACZO9R_09465"
     CDS             complement(240..1844)
                     /locus_tag="ACZO9R_09465"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR0913324.1"
                     /translation="MKKKIGITAAVILVILAVCYIGFAVFFQSHFCYGTTIDGIKVGG
                     CNTAKVEQLIEKEIGEYEMTLVEREDQTETITASQIGAAPVFHGEIEELLAHQNAFAW
                     PVILFGKSALELEKTVAFDDTKFSGTIEALSCMQEENQRKPVDASCSGYSAADGYTLV
                     PADYGTTIDETALKNAVAEAVEGLEDTLDLEKSGCYVDPAVGDDDKDLLAVIDELNQY
                     VASTVTYDFGDQKEVVDGSTISEWLSVLDGELEVDEEAVLDYVKGLAKTYNTAYKPKT
                     LKTSYGPEVTISNGAYGWKIDTEGEEAQLLEDIKSGKSVEREPVYSQTANSHGENDYG
                     NSYVEINLTSQHLFVYKNGSLVVDSDFVSGNLSKGHGSPTGAFSVTYTTTDAVLRGED
                     YATPVKYWMPFAGDVGMHDASWRKSFGGNIYKTNGSHGCINLPTSVAKTIYNTIEKGW
                     PVLVYTLPGTESAAQLQQDVQTVIDLINSIGEVTADSETVIASARSQYDALPDSTKAN
                     VTNYDVLVAAEASLAQIKAAGEQTGM"
BASE COUNT          395 a          584 c          422 g          527 t
ORIGIN      
        1 gcacgaaagt cctacaaatt gtctccggga tgcggacatg acccgcggag tgctccaaac
       61 ttggaaagac acggatcatc gaagatgcaa acatccggct ggttagaaat gccatatttt
      121 ttgatccgaa tttccacctg catttccatc tgaaatcccg cggtgtgatc ttctgataat
      181 attacttatc acccaaaaga ggtctatgct tattgcgaca attccatgcg gcagacagac
      241 tacattcccg tctgctctcc cgccgctttg atctgtgcca gtgatgcctc ggctgccacg
      301 agcacatcgt aatttgtcac attcgccttt gtggaatccg gcagcgcatc atactggctg
      361 cgtgctgacg cgatgactgt ctcactgtcc gccgtcactt cgccgataga attgatcaga
      421 tcgatcacgg tctgcacatc ctgctgcagc tgtgcggcgc tctctgttcc cggaagcgtg
      481 tacaccagta ccggccagcc cttctcgatc gtattgtaga tcgtctttgc cacggatgtc
      541 ggcagattga tgcatccgtg cgatccgttt gtcttataaa tattcccgcc gaagcttttt
      601 ctccagctcg cgtcatgcat gccgacgtct cccgcaaacg gcatccagta ttttaccggc
      661 gtcgcgtagt cctctccgcg cagcacggca tccgtggtcg tataggtcac gctgaacgct
      721 ccggtcggag aaccgtgtcc ctttgacaga tttcccgata caaaatcgct gtccacaacc
      781 agacttccgt tcttgtatac aaacagatgc tgcgatgtca ggttgatctc cacgtaggaa
      841 ttgccgtagt cattctcgcc atggctgttc gctgtctggg aatagaccgg ctcacgctcc
      901 acgctcttgc cggacttgat gtcctcaagc aactgcgcct cctctccttc tgtgtcaatc
      961 ttccagccgt aagcgccgtt tgagatcgtt acttccggtc cataagacgt ctttaacgtc
     1021 ttcggtttgt acgcggtatt gtaggtcttt gcaagtccct tgacatagtc gagcaccgcc
     1081 tcctcgtcga cctccagttc accatccaga accgagagcc actcgctgat cgtactgcca
     1141 tccacaactt ccttctgatc cccgaaatcg taggtgaccg tcgatgcaac gtactggttt
     1201 aattcgtcga tcacggcgag aagatctttg tcgtcgtctc ccaccgccgg gtccacataa
     1261 cagccgctct tctccaggtc gagtgtgtcc tccagccctt ccacggcttc tgccaccgcg
     1321 tttttcaacg ctgtctcatc gatcgtcgtt ccgtaatccg ccgggacaag cgtatagccg
     1381 tccgctgcgg aatagccgga acaggacgca tccaccggct ttctctgatt ctcctcctgc
     1441 atgcaggaaa gcgcttcgat cgtcccggaa aactttgtgt cgtcaaaggc aaccgttttt
     1501 tccagttcca gtgcactctt tccaaacaga atcaccggcc atgcgaaggc gttctgatgc
     1561 gccagcagtt cttctatctc cccgtgaaaa accggcgccg cgccaatctg tgatgccgtg
     1621 atggtctctg tctgatcctc gcgctccacc agcgtcatct catattctcc aatctccttt
     1681 tctatgagct gctccacctt tgcggtattg catcctccca ccttaatgcc atctatcgta
     1741 gtgccatagc agaagtggct ctggaaaaac accgcaaatc cgatatagca gactgcgagt
     1801 attaccagaa tgactgccgc ggtaatcccg attttctttt tcattttctg tttcctttcc
     1861 tcttatcgta aacatttata tgcctgttct ttcagtgaaa acacatggtt tattataaat
     1921 gacccttt
//
LOCUS       DBMDQJ010000488         1927 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20205_length_1927_cov_1.581599, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000488 DBMDQJ010000000
VERSION     DBMDQJ010000488.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1927)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20205_length_1927_cov_1.581599"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="ACZO9R_09470"
     CDS             complement(<1..512)
                     /locus_tag="ACZO9R_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HIR0913325.1"
                     /translation="METVIAVVVAIAVTAIVVYFVTSAYHKKVTEAKIGNAEEKAREI
                     IDEAVKTAETKKREAMLEAKEESIRVKNDLDKEVKERRAEIQRSERRVVQKEENLDKK
                     LESIERREAGFAAKEEEINKQKAQVAKLNEERLQELERISGLTSEQAKEYLLKTIEED
                     VKLDTAKLIK"
     gene            complement(708..1355)
                     /locus_tag="ACZO9R_09475"
     CDS             complement(708..1355)
                     /locus_tag="ACZO9R_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR0913326.1"
                     /translation="MQEVQVKEVARREKGRVFFRFDNGTEFILYRSEIRGLPQAESTL
                     LMTEDAYLPVSLYEKILTEIVGKRAKKRALFLLERMDRTESQLCEKLRQNGYPEECVA
                     AAVEYVKQYHYIDDLRCAKQYIRYHQQQKSSQRLKMDLMKKGVGREQIEQALAEEFVS
                     DERAQIAELLEKRHYDAAQADRRERQRMYQFLMRRGFKSSDILAVMREEHFFDEF"
     gene            complement(1380..>1927)
                     /locus_tag="ACZO9R_09480"
     CDS             complement(1380..>1927)
                     /locus_tag="ACZO9R_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140664 - ATP-dependent DNA damage sensor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA family protein"
                     /protein_id="HIR0913327.1"
                     /translation="KLTSIVSKSNCIVIFINQLREKIGVMFGNPETTTGGRALKFYST
                     VRLDVRRVEALKQGGEVVGNHTRVKIVKNKVAPPFKEAEFDIMFGQGISKEGDILDLA
                     ADAGIINKSGAWYAYNGEKIGQGRENAKNYLKENPLICEEVEAKVRAKYSPEEDEAAE
                     QPDEKSAGKTAGRTPEELPAE"
BASE COUNT          419 a          581 c          349 g          578 t
ORIGIN      
        1 tctttgatca attttgcagt atcaagtttt acatcttctt caatggtttt taagagatat
       61 tcctttgcct gttcggaggt taatccggag attctttcca gttcctgtaa ccgctcttca
      121 ttgagctttg cgacctgcgc tttctgtttg ttgatctctt cttcctttgc agcaaaaccc
      181 gcttcgcgtc tctcgattga ctcaagcttc ttgtccagat tctcttcctt ctgtacaaca
      241 cgacgctcgc tgcgctgaat ctctgcccgg cgttctttta cttccttatc cagatcgttc
      301 ttcacacgaa tggattcttc cttcgcctca agcattgcct cgcgcttctt ggtctccgct
      361 gtcttcaccg cctcatcaat aatctctctt gccttctctt ctgcgttccc aatcttggct
      421 tccgtcacct tcttatggta agcagatgta acaaaataga ccacgatggc agtcactgcg
      481 atagcaacaa caacagcaat cactgtctcc acaggagcac ctcctttatt aagttttcat
      541 tgtacatata acacatataa cataaaaata taatcgttat ctgtcataaa ttacaacaca
      601 taaattttat tctatttcat gaattatgtc aagtgaataa ctgtacaata tgcataatat
      661 tgcatttcat ttttacaaaa atacaagata ttgtactgaa atttatttta aaattcgtcg
      721 aaaaaatgct cttcgcgcat cactgcaaga atatcactgc tcttaaaacc gcgcctcatg
      781 aggaactgat acatccgctg ccgttccctg cggtctgcct gcgccgcatc gtagtgccgc
      841 ttctctaaaa gctccgcaat ctgtgcccgc tcatccgaca caaactcctc cgcgagtgcc
      901 tgctcaatct gctcccgccc tacccccttt ttcatgaggt ccatctttaa gcgctggctg
      961 ctcttctgct gctggtgata gcggatatac tgcttcgcac aacgcaggtc atcgatatag
     1021 tgatactgct ttacatactc caccgccgcc gcaacgcact cctcgggata accgttctga
     1081 cggagctttt cacagagctg cgactccgtg cggtccatac gctccagcag aaacagtgca
     1141 cgctttttgg ctctctttcc gacaatctcc gtcagaatct tctcatatag tgataccggc
     1201 agatacgcat cctccgtcat caaaagcgtg ctctctgcct gcggcagtcc ccggatctcg
     1261 ctccggtaga gtataaattc ggttccgttg tcaaaccgga aaaacacccg tcccttctca
     1321 cgtctggcta cttcctttac ctggacttcc tgcatgatct gtctgtcgca ctcctatcct
     1381 tattctgccg gcagttcttc cggagtacgt cctgctgtct ttcccgcact cttctcatcc
     1441 ggctgttctg cagcctcatc ttcctcaggg ctgtactttg cacgtacctt cgcttccact
     1501 tcctcacaga tcagcggatt ctccttcagg tagttcttgg cattctcacg tccctggccg
     1561 atcttctcgc cgttgtaggc ataccatgca ccggatttgt tgatgatgcc ggcatccgcc
     1621 gccagatcaa gaatatctcc ttccttggaa ataccctgtc cgaacataat atcgaactcc
     1681 gcctccttga aaggcggtgc caccttgttc ttcacaatct tcacacgcgt atggttgccg
     1741 acaacctcac cgccctgctt taacgcctcc actctccgca catccagacg caccgtagaa
     1801 tagaacttca gcgcacggcc gccggttgtc gtctccggat taccgaacat aacgccgatc
     1861 ttctcacgca gctggttgat aaaaatgacg atacagttcg acttgctgac aatcgatgtc
     1921 agcttac
//
LOCUS       DBMDQJ010000489         1926 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20214_length_1926_cov_1.818630, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000489 DBMDQJ010000000
VERSION     DBMDQJ010000489.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20214_length_1926_cov_1.818630"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            157..1218
                     /locus_tag="ACZO9R_09485"
     CDS             157..1218
                     /locus_tag="ACZO9R_09485"
                     /inference="COORDINATES: protein motif:HMM:NF024242.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0913328.1"
                     /translation="MLLAEMPEEELTMLETQYDQHFILDLSGKPYVVCCRNRKKEEES
                     CPKDCLFLGDVEGNDLFLIEAEALSDRPGEYPFLQEYTGISRPHQGIRELREAYLEAR
                     EMRRCAFCTNRSQMRYGQEMPRVPQKLVQEASKLVADEMKLQRVQLLGTDRTEELQHV
                     WTQFFYEVKHGRIDVRDFEECMTDFLTETSKTYRNVLEEKENCGEIKEITDPFGEDAI
                     DRYEQKVLAFVTGLQARILSQFDTNGNQQKMKQAVAYIEEHYASDLNMAVVSNYLSMN
                     YSLFSYSFKQYTGSNFVNYLRDIRMKEAKRLLAGTDMRIAEISQAVGYENEKHFMKLF
                     KSSCGVSPSEYRRNMGLTG"
     gene            1339..1866
                     /locus_tag="ACZO9R_09490"
     CDS             1339..1866
                     /locus_tag="ACZO9R_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0913329.1"
                     /translation="MSSTKTTSTEQVRFLTVTAIGIALVYVFTWLINIRLPFAPQGGL
                     IHLGNVPLFIFAILFGKKTGAIAGGVGMGLFDLLSGWTAWAPFTFVIVGCMGFVMGWI
                     TEKKQGFQWYVVAMLAACAIKIGGYYIAEGIIYGNWITPVTSIPGNLLQIGTAAVIVL
                     IIIAPLKKVAAKLWN"
BASE COUNT          529 a          419 c          555 g          423 t
ORIGIN      
        1 cagaagcgag aaacagcagc agtactatcg ctatcctaac cgatcgagcg cgaaaaaatc
       61 tgcgacatac ttgccaggct gaatcaggag attgaggagg gcagggagca ggagaaaacg
      121 cgccagaagc tcggacagca gcagatccgt catctgatgc ttctggcaga gatgccggaa
      181 gaggagctta ccatgttgga gacccagtat gaccagcatt ttattttaga tctgagcgga
      241 aagccgtatg tggtctgctg caggaaccgg aaaaaagagg aggaatcctg cccgaaggac
      301 tgcctgttcc tgggcgatgt cgagggaaat gatctctttt taatcgaggc ggaagcactt
      361 tcggaccgtc cgggggaata tccttttttg caggaatata ccggaatcag ccggccgcat
      421 cagggaatcc gcgaactgcg cgaggcatat ctggaggcgc gggaaatgcg caggtgcgcg
      481 ttttgcacga accgcagtca gatgcgctat gggcaggaga tgccgcgtgt cccacagaag
      541 ctggtgcagg aggcgtcaaa gctggttgca gacgagatga aactgcagcg ggtgcagtta
      601 cttggaaccg accgcaccga ggaactgcag cacgtctgga cgcaattttt ttacgaggta
      661 aagcatggcc gcatcgatgt ccgggacttt gaggagtgca tgacggattt ccttacggag
      721 acgtcaaaaa cgtaccggaa tgtgctggag gaaaaagaaa actgcgggga gatcaaagag
      781 attacagacc cattcgggga ggacgcaatc gaccgctacg aacagaaggt gctggcattt
      841 gtgaccggtc tgcaggcgcg gattttaagc cagtttgaca caaacgggaa tcagcagaaa
      901 atgaagcagg cggtcgccta tatcgaagaa cactatgcca gtgatctgaa tatggcggtt
      961 gtctccaatt atctgtccat gaattattcc ctgttttctt attcgtttaa gcagtatacc
     1021 ggaagtaatt ttgtcaatta cttaagagac atccgcatga aggaggcgaa gcgtctgctc
     1081 gccggaacgg atatgcgcat cgcggagatc tcccaggcag tcggctatga gaacgagaag
     1141 cattttatga agttgtttaa gagcagctgc ggcgtttcac caagcgaata ccggagaaat
     1201 atggggctta ccgggtaaaa agatcgaact ggactggtca aaaatagcaa aactggacat
     1261 attcattcgt ccagaaatgg ggtatcatgg ctctaacaaa aaagaaagag agatagagcc
     1321 atgtcagaga ctaaaactat gagcagcaca aaaacaacca gcacagagca ggtgcgtttt
     1381 ctcaccgtca cagcgatcgg aatcgcactg gtatatgtat ttacctggct gattaacatt
     1441 cgcctgccgt ttgcgccgca gggcggactg attcatcttg gaaatgtccc actgtttatt
     1501 ttcgcaatcc tgtttggcaa aaagacggga gccattgccg gcggagtcgg catgggactg
     1561 tttgatctgt tgtccggctg gacggcatgg gcaccgttta cctttgtgat cgtcggatgc
     1621 atgggatttg tgatgggatg gattacagag aagaagcagg gctttcagtg gtatgttgtg
     1681 gcaatgcttg ctgcctgcgc gatcaagatc ggcggatatt acatcgcaga gggaattatt
     1741 tacggcaact ggatcacacc ggtgacatcg attccgggca acctgcttca gatcggaaca
     1801 gcagctgtga tcgttcttat catcattgca ccgttaaaga aagtagctgc aaaactgtgg
     1861 aactaaggag gcgtttccat gtataagatt atgacaccgg gacctacgca ggtcaaagag
     1921 aatgtc
//
LOCUS       DBMDQJ010000490         1925 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20225_length_1925_cov_1.582237, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000490 DBMDQJ010000000
VERSION     DBMDQJ010000490.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20225_length_1925_cov_1.582237"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="ACZO9R_09495"
     CDS             <1..794
                     /locus_tag="ACZO9R_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913330.1"
                     /translation="DAAREAVIATQGEVLSYQGNIVEAYYFSTSMGYTAAADVWNVED
                     PAEYGYLTPACLLTDGKMQDLSGEEAFLAYIQSPADGYDSDCRYFRWRAEADYHGKTD
                     EVNSILLERRKSSPKNIIFYQDGQTTEIQNSDAASVAALGEVTGMSAAERGSSGALLA
                     LKITYEKGSALVRTEYNIRKVLGICTAKLTCADGAEQTDVTMLPSAFFAITKQEDGGM
                     VLYGGGYGHGLGMSQNAANGMAKAGMNYEEILQYFYNDVKLETMK"
     gene            832..1686
                     /locus_tag="ACZO9R_09500"
     CDS             832..1686
                     /locus_tag="ACZO9R_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR0913331.1"
                     /translation="MIWKLIGNVKSFLDKCKRDKINAYSAQSAFFIILSIIPFLMVFS
                     SLLQYTSVTESMLLEIIRHLMPEYVSPFLISLVDEVYSRSMGIISITAIIAIWSAAKG
                     VQYMTDGLNSVNDLEETRNWFVLRFWAVVYTVVFLVAIVFTLLVLVFGNQLKVLATQY
                     VPILEHASRVLAHFRGLVMLALLIAFFDVIFTALPNRKLSFRGQLPGAVICGVAWYVF
                     SFGLSVYVDYFNGFSMYGSLTTIALIMLWLYFCMYIMMMSAEVNVVFNAYFRKWWKRF
                     KTSRNKKA"
BASE COUNT          459 a          403 c          569 g          494 t
ORIGIN      
        1 cggacgcagc cagggaggcg gtaatcgcca cacaggggga agtgctgtcc tatcagggaa
       61 acatcgtgga ggcgtattat ttttccacgt cgatgggata caccgccgca gccgatgtgt
      121 ggaatgtgga agatccggcg gaatacggct atctgacacc ggcgtgtctc cttacggatg
      181 gaaagatgca ggatctctcc ggggaggagg cgtttcttgc ttatattcag agtcccgcag
      241 atggatacga cagtgactgc cggtatttcc gctggagagc ggaggcggat taccatggga
      301 aaacagatga agtgaattcc atactgctgg aacggcggaa gagttcgcca aagaatataa
      361 tcttttatca ggacggtcag actacggaaa tacagaattc cgatgcggca tcagtggcgg
      421 cactggggga ggtgaccggc atgagcgcgg cggagcgcgg cagctccggt gcactgcttg
      481 ccttaaagat cacgtatgag aagggaagcg cccttgtgcg gacggagtat aatatccgaa
      541 aagttctggg gatctgtacc gcaaaactga catgcgcaga cggcgcggaa cagacggatg
      601 tgacgatgct gccgagtgcg ttttttgcca taacgaaaca ggaggacggc ggcatggttt
      661 tatacggagg cggctacggg catggactcg gcatgagcca gaatgcggcg aatggaatgg
      721 cgaaggcagg aatgaattac gaagagatct tgcaatattt ttataatgac gttaaactgg
      781 aaaccatgaa ataatgaatc ctgacggatt cgctttttga aggggagcaa tatgatctgg
      841 aagcttattg gtaatgtcaa atcctttctt gataaatgca agagggataa gatcaatgcc
      901 tattcggcgc agtcggcgtt ttttattatc ttatccatca ttccgtttct gatggtgttc
      961 tcgtctctgc tgcaatatac atcggtgaca gagtccatgc tgcttgagat catccgtcat
     1021 ctgatgccgg agtatgtctc tccgtttctg atatcgctcg tggatgaggt gtacagcaga
     1081 tccatgggaa ttatctcaat cacggcgatc atcgcgatct ggtcagcggc caagggcgtc
     1141 cagtacatga cggacggact gaactcggtc aatgatctgg aggagacgag gaactggttt
     1201 gtgctcagat tctgggcggt cgtctatacg gtggtttttc tggtggcgat tgtttttacc
     1261 ctgctggttc tggtgttcgg aaaccagtta aaggtacttg ccacacagta cgttccgatt
     1321 ttagagcatg cctcgcgggt gctggcacat ttccgcgggc ttgtgatgct ggcgcttttg
     1381 attgcatttt tcgatgtaat ctttacggcg ctgccgaacc ggaagctttc cttccgcggg
     1441 cagctgccgg gggctgtgat ctgcggggta gcgtggtatg tcttttcctt cggactgtct
     1501 gtctacgtgg actattttaa cggtttttcc atgtacggca gcctgacgac tattgccttg
     1561 attatgctgt ggctttactt ctgcatgtac atcatgatga tgtccgccga ggtaaatgtg
     1621 gtgttcaacg cttacttccg caaatggtgg aagcgcttta aaaccagcag gaataaaaaa
     1681 gcttgatgtt ttgcgcctgc tgtgctaaaa tgctatacag aattcagaaa aatcaaaggc
     1741 tgtgaatgtg tacggaagcg attccgggta tcgtaagata tcagtagagt gcaggagact
     1801 ctttcagaga gagcgggtca tcggctggaa gcccgtttag gataagacct ggacttgaag
     1861 ttcgcacagg agctgcatct gtgaagtgaa agtagtggat gacgtcggat acacgttacg
     1921 tatga
//
LOCUS       DBMDQJ010000491         1925 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20226_length_1925_cov_1.511513, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000491 DBMDQJ010000000
VERSION     DBMDQJ010000491.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20226_length_1925_cov_1.511513"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(58..999)
                     /locus_tag="ACZO9R_09505"
     CDS             complement(58..999)
                     /locus_tag="ACZO9R_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015514533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0913332.1"
                     /translation="MYLVNALNVIDEVKKAVIGKDSCVEMVMMAILARGHILIEDIPG
                     VGKTTMALAFSRACAMSQNRVQFTPDVLPADITGFSIYRKDTGKFEYQPGAVMCNLLL
                     ADEINRTSPKTQSALLEVMEEGNVTVDGITREVPKPFIVMATQNPIGSSGTQMLPESQ
                     LDRFMICISMGYPDIRNEIAILKDHRSGSPVDRISPVIQMNDLLTMQEEVDKIFIHDV
                     IYNYIAELIARTRQHPMLELGVSPRGTIALAGMIKAAAYLAGRNYVVPNDVEKVFLAV
                     NHHRIRLNSKARANHVTAEGVLEEIFAGVAKPSPKKN"
     gene            complement(1070..1552)
                     /locus_tag="ACZO9R_09510"
     CDS             complement(1070..1552)
                     /locus_tag="ACZO9R_09510"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015542294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR0913333.1"
                     /translation="MDKRTDYISWDEYFMGVAHLAALRSKDPNTQVGCCIVSEDNKIL
                     SIGYNGFPKGCSDDEFPWRREGDPYDTKYFYTTHSELNAILNYRGGSLEGAKLYVSLF
                     PCNECAKAIIQCGIRTVVYDCDKYDGTPSVRASKRMMNAAGVRYYKYQKTGRNIEISV
                     "
     gene            complement(1595..>1925)
                     /gene="upp"
                     /locus_tag="ACZO9R_09515"
     CDS             complement(1595..>1925)
                     /gene="upp"
                     /locus_tag="ACZO9R_09515"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR0913334.1"
                     /translation="GLYRDPKTLEPVEYYCKLPADSEEREVFVVDPMLATGGSATAAI
                     QMLKDKGVKHIHFMCIIAAPEGVKKMQECHPDVDMYIGALDEQLNSHGYIVPGLGDAG
                     DRIFGTK"
BASE COUNT          431 a          519 c          410 g          565 t
ORIGIN      
        1 tattgtgata agaaccaata tcatatacca tacttttctt atcataccat acctccgtta
       61 attctttttc ggtgatggtt ttgccacgcc ggcgaaaatc tcttccagaa ctccttctgc
      121 tgtcacatgg ttggctcttg ccttggagtt taaacggatt cggtgatggt tgactgccag
      181 aaataccttc tccacatcgt ttggcacgac ataatttctt cccgccagat aagcagccgc
      241 cttgatcatc cccgccaggg cgatcgttcc tcgtgggctt acaccaagct ccagcatcgg
      301 atgctgtctg gtccttgcga tcagctctgc aatataattg tagatcacat catggataaa
      361 gattttgtcg acttcttcct gcattgtcag cagatcgttc atctggatca ccggtgaaat
      421 gcgatctacc ggactgccgc ttctgtggtc ttttaaaatg gcaatttcgt ttcggatatc
      481 cgggtagccc atggaaatac agatcataaa gcggtccagc tgggattccg gcagcatctg
      541 tgttcctgat gagccgatcg ggttctgtgt cgccatgacg atgaacggct tcggcacttc
      601 tcttgtgatg ccatccaccg tcacatttcc ttcttccatc acttccagca gtgcggactg
      661 tgttttcggg gatgtacggt tgatctcgtc tgccagaagc aggttgcaca tcaccgcccc
      721 cggctggtac tcgaatttgc cggtgtcctt gcggtaaatg gaaaatcctg tgatatctgc
      781 cggcagcaca tccggtgtga actgcacacg gttctggctc atggcacatg cccttgaaaa
      841 tgccagtgcc atcgtggtct ttcccacacc cgggatatcc tcgatcagaa tgtgtcccct
      901 cgcaagaatg gccatcatca ccatttccac gcaggaatct tttccaatga ctgctttttt
      961 gacttcgtca attacattta atgcatttac cagatacata gtttcctcct gaaaaatgcc
     1021 acagaaccct ctcttctcta tgagattgtc ctgcggcatt tttgtcacat tatactgaaa
     1081 tttcaatatt tcttccggtt ttctgatatt tataataccg cacgcctgcc gcattcatca
     1141 tacgtttgga tgcacgcacg ctcggcgtac catcgtattt atcgcagtcg tatactacgg
     1201 ttcggatccc gcactgaatg atcgctttgg cacactcgtt gcacggaaag agcgaaacat
     1261 aaagctttgc tccctccagg ctgcctcccc ggtaattgag gattgcattg agttcgctgt
     1321 gcgttgtata aaaatacttg gtatcgtacg gatctccttc tcttctccac gggaattcat
     1381 catcggaaca tccctttgga aatccattat atccaatgga caggatttta ttatcctcgc
     1441 tcacaataca gcagccaacc tgtgtatttg gatctttgga tctgagtgcc gcaagatgtg
     1501 caacccccat aaaatactcg tcccaggaaa tatagtcggt tcttttgtcc atactttcct
     1561 ccaaatgacg ttctccgtca gactcaggca acgtctattt tgtgccgaag atacggtctc
     1621 ctgcatcgcc aagtcccggt acgatatagc cgtgggaatt gagctgttca tccaatgcac
     1681 cgatgtacat atccacatcc ggatgacatt cctgcatctt ctttacacct tccggtgctg
     1741 cgatgatgca catgaaatgg atgtgcttta cgcctttgtc tttgagcatc tggatcgccg
     1801 cagttgcaga tccgccggta gccagcatcg gatctacgac aaaaacttca cgctcttcag
     1861 aatctgccgg cagtttgcag taatattcca ccggctccag tgtcttggga tcacggtaca
     1921 gaccg
//
LOCUS       DBMDQJ010000492         1921 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20270_length_1921_cov_1.635165, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000492 DBMDQJ010000000
VERSION     DBMDQJ010000492.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20270_length_1921_cov_1.635165"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="ACZO9R_09520"
     CDS             <1..401
                     /locus_tag="ACZO9R_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913335.1"
                     /translation="LLADQEAENPILVGNVWHNMGKAYTGLFRFREAADCYRKAYGLN
                     ENPESLRECLYAYRCLHDDDGFKNTAAECGMTAEEAAEAAHRLSELSRTEEIRQFEEQ
                     VDGLFADGQEDEIAGMLAEWKDTYRKNCRI"
     gene            465..1586
                     /locus_tag="ACZO9R_09525"
     CDS             465..1586
                     /locus_tag="ACZO9R_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0913336.1"
                     /translation="MGELNTTVLSKKDYKNTGKIEQYVVERLEQMIELTKELDDEKEE
                     YRIVTSYLNDIETLEKIPKEERDKISEIAVNVVQLNKARTEFLNSSKKLSDAQFTQLE
                     QQERELPEAIKRFKTNELHRDTLQRDMKYLEREKSEWNLRKEYLDHQQKKLKNVLYII
                     VGLAVTLAVVFGILQIILEKDFYYAWMGLIFATAVVVCGIYMKIQNDLSEIQVAERSQ
                     NRAIVLLNKVKLKYINVANAVDYACEKYHVKSAGELENQWQYYLEAVKERQKYQRINE
                     DLEYFNGRLVRMLSNYQLYDAHVWVTQAVALVDPKEMVEIKHGLIGRRQKLRARIENN
                     LSQVNEQKEEAEQLLDKVGSMRPQMEKILNAVDKINEGD"
     gene            1645..1866
                     /locus_tag="ACZO9R_09530"
     CDS             1645..1866
                     /locus_tag="ACZO9R_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006442087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HIR0913337.1"
                     /translation="MRNYNAAGNTRSRCACEPDAASDRQPEYVVAMAYVPWQHFKTTY
                     ELDKALETGTIFPELDKPFLAVGRGCRRC"
BASE COUNT          585 a          359 c          573 g          404 t
ORIGIN      
        1 cattgcttgc ggatcaggaa gcggagaatc cgatcctggt cgggaatgtc tggcataaca
       61 tgggaaaagc gtatactgga ttattccggt ttcgggaagc agcggactgt taccggaagg
      121 cgtacggact caatgaaaat ccggaatctc tccgcgaatg tctctatgcg taccggtgtc
      181 tgcacgatga tgacggattc aagaatacgg cggcggaatg cggcatgacg gcagaggagg
      241 cggcggaggc ggcgcacaga ctttccgagc tttcccggac ggaggagatc cgtcagtttg
      301 aagagcaggt ggacggactg tttgccgacg ggcaggaaga tgaaattgca ggaatgcttg
      361 cagagtggaa agatacctat cggaaaaatt gcagaatata gaggaagcgt gaaacgatgt
      421 ttttttggaa gagaaagaaa aaggaacctg attttgagag taagatggga gaattaaata
      481 caacggttct ctcaaaaaag gattacaaaa ataccggtaa gattgaacag tacgtggtgg
      541 agcgcctaga gcagatgatt gagttgacca aggagctgga cgatgagaag gaagaatacc
      601 ggattgtgac gtcctatctg aacgatattg agactctgga gaagataccg aaggaagaaa
      661 gagataagat ttctgagatc gcggtaaatg tggtgcagtt aaacaaggcg cgcacggaat
      721 ttctcaactc ctccaagaag ttgtcggacg cgcagtttac acagttagag cagcaggaac
      781 gcgaactgcc ggaggcaatc aaacgtttta agaccaacga actgcaccgt gacacgctac
      841 agagggatat gaagtatctg gagcgggaaa agtccgagtg gaatctgcgc aaggaatacc
      901 tggaccatca gcagaaaaag ttaaaaaatg tgctgtatat tattgtcggt ctggcggtga
      961 cgctcgcggt ggtgttcgga attttacaga ttattctgga aaaggatttc tactatgcgt
     1021 ggatgggact gatctttgca actgcggtgg tggtgtgcgg tatttacatg aaaatccaga
     1081 atgacctgtc ggagatacag gtggcggagc ggagccagaa ccgtgcgatt gttctgctta
     1141 acaaggtcaa gctgaaatat atcaatgttg ccaatgcggt cgattatgcg tgtgaaaaat
     1201 accatgtaaa aagtgctgga gaactggaaa accagtggca gtattatctg gaggcggtca
     1261 aggaacgcca gaaatatcag agaatcaatg aggatctgga atattttaac ggcagactgg
     1321 tgcgcatgct ctccaattat caactgtacg atgcgcatgt gtgggtaaca caagcagttg
     1381 cgctcgtcga tcccaaggag atggtggaga tcaagcacgg actgatcggg cgcagacaga
     1441 agctgcgcgc acggattgag aacaatctct cgcaggtcaa cgagcagaaa gaggaggcag
     1501 aacagctttt agacaaggtc ggaagcatgc gcccccagat ggagaagatt ttaaatgcag
     1561 tagataaaat taacgaggga gattagcaca agtgaatggg atgcggcata gagtaaaggg
     1621 aagatatcat aataggagag ccgcatgaga aattacaatg cagcaggaaa cacgcgcagc
     1681 cgatgtgcgt gtgaaccgga tgcagcttct gatagacagc cggaatatgt ggttgcgatg
     1741 gcatatgttc cctggcagca ctttaaaacg acctacgaac tggataaggc acttgaaacc
     1801 ggaaccatat tcccagaact tgataagccg tttctggcag tgggaagggg gtgcaggcgt
     1861 tgctgatgaa tcagatgaca cagatgaacc aggcgcagct tctgcactgg atcaatatgg
     1921 t
//
LOCUS       DBMDQJ010000493         1917 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20327_length_1917_cov_1.764317, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000493 DBMDQJ010000000
VERSION     DBMDQJ010000493.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20327_length_1917_cov_1.764317"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(56..643)
                     /locus_tag="ACZO9R_09535"
     CDS             complement(56..643)
                     /locus_tag="ACZO9R_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR0913338.1"
                     /translation="MVRVYKVPVLKPVMDVLMALSKAFPMNLVLLIFYLIWTTQFSSI
                     AAFFHSQKTIKDVSLMYVAIAALVFCSLASISEIIRSGLISVDKGQYEAGYASGLTKA
                     QTFFHVIVPQAVRAVIPPLTNDILSLVKGTALVSVIGVSDILTDSINAASAAYSYLEA
                     YVAAAMVFWGIGIVLERLSHYVERKFSKSVKTLAG"
     gene            661..996
                     /locus_tag="ACZO9R_09540"
     CDS             661..996
                     /locus_tag="ACZO9R_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913339.1"
                     /translation="MDYVASEFCEIGVLLKNRENRVLDWEMEQQELDFHLLATMRPKV
                     YVPKQHPLAGRAKVSMEYLAPYVYSHYFQGIDSSRDRFFSEELVENTAAKKQSPSRTR
                     WQMPASAWR"
     gene            966..1187
                     /locus_tag="ACZO9R_09545"
     CDS             966..1187
                     /locus_tag="ACZO9R_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913340.1"
                     /translation="MADASIGMEMNTYTIGSGMSGENLLEKDYAVMNLDTHQRIELGW
                     ISRRDHELSNFGKRFLDLLAEKLNSMQLD"
     gene            complement(1327..1452)
                     /locus_tag="ACZO9R_09550"
     CDS             complement(1327..1452)
                     /locus_tag="ACZO9R_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913341.1"
                     /translation="MSDKNPKHPLKKKKVAAKVNMADHEAVEEAREAHKSKKHVY"
     gene            complement(1483..>1917)
                     /locus_tag="ACZO9R_09555"
     CDS             complement(1483..>1917)
                     /locus_tag="ACZO9R_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002573087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR0913342.1"
                     /translation="LFDDWFDDFPFFDDRDLRKVDKKLYGRRAGNLMKTDIQEMKDGY
                     KMEVDLPGFKKDEITVELDDGYLTISAAKGLDQDEKEKESGRYIRRERYAGACSRSFY
                     VGEGVTEEDIKAEFKHGILTLVVPKKEAKPAVEQKKYIAIEG"
BASE COUNT          459 a          542 c          410 g          506 t
ORIGIN      
        1 ctgccatagc cctgtttcct tcctattata ataatgtacg atgccgtacc gcatctcagc
       61 ctgccagtgt cttaacgctt tttgaaaatt tccgctccac atagtggctc agccgctcta
      121 acacaattcc gatgccccag aataccatgg ctgccgccac atacgcctcc aggtaagagt
      181 acgccgcact tgccgcgttg atggaatccg tcagaatatc tgacactccg atgaccgaca
      241 ctaacgccgt tccctttacc agcgagagaa tatcgttcgt gagcggtgga atgaccgccc
      301 gcaccgcctg cggcacaatc acatggaaaa aggtctgtgc ttttgtcagt cccgaggcat
      361 atcccgcttc atactgtccc ttgtccacgg agatcagacc gcttcttatg atttccgaaa
      421 tactggcaag actgcagaac accagagctg caatcgccac atacatcaga ctgacatcct
      481 tgatcgtctt ctgtgaatga aaaaatgctg cgatactgga aaactgcgtc gtccagatca
      541 gataaaagat gagcagaacc agattcatcg gaaacgcttt gctcagtgcc ataaggacat
      601 ccataaccgg ctttagcacc ggcactttat agactctcac cattgcgcac caaggagatc
      661 ttggattacg tcgcatcgga attctgtgag atcggcgttc tgctgaaaaa ccgtgagaac
      721 cgcgttctcg actgggagat ggagcagcag gaacttgatt ttcatctgct ggcaaccatg
      781 cgccccaaag tctatgttcc caagcagcat ccgctcgccg gcagagcgaa ggtatcgatg
      841 gaatacctgg ctccctatgt ctacagccac tattttcagg gcattgacag ttccagagac
      901 cgcttcttct ccgaggagct tgtggagaat accgccgcaa aaaaacaatc accctcacgg
      961 acgagatggc agatgccagc atcggcatgg agatgaacac ctataccatc ggttccggca
     1021 tgtccggtga gaatctgctg gaaaaagatt acgcggtcat gaatctggac acgcatcaac
     1081 ggatcgaact tggctggatc tcccgcaggg atcacgaact aagtaatttc ggcaagcgct
     1141 ttctggatct gctggcagaa aaactcaact ccatgcagtt ggattagcga ataggtaatc
     1201 tttggacacg tttttgtgta ataggaacga agtttcctat tacacgaaaa agggacaggg
     1261 ggcgcacagc acttcgtgct gtgcagctcc ctgtcccttt tctcctgtca tctgcaggcg
     1321 tatcgcttag taaacatgct ttttcgactt gtgggcttct ctcgcttcct cgaccgcctc
     1381 atggtctgcc atattcacct ttgctgctac ttttttcttc ttcaacggat gttttggatt
     1441 cttatctgac atgatcatac gcttcctttc tctctgtcat ccttatcctt caatcgcaat
     1501 gtatttcttc tgctctactg caggctttgc ttccttcttc ggaacaacca gcgttaagat
     1561 accgtgcttg aactcagcct tgatatcctc ctcggttacg ccctcgccca catagaagct
     1621 ccggctgcag gcacctgcat aacgctctct gcggatgtaa cgtccggatt ctttctcctt
     1681 ctcgtcctga tccagtccct tggcggcact gatggtcaga tatccgtcat ccagctctac
     1741 ggtaatctca tccttcttga agcccggaag atcaacttcc atcttgtagc catccttcat
     1801 ctcctgaata tctgtcttca tcaggttacc ggctcttctg ccgtacagct tcttgtcaac
     1861 cttgcgcaaa tctctgtcat cgaaaaatgg aaaatcatca aaccagtcat caaataa
//
LOCUS       DBMDQJ010000494         1917 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20332_length_1917_cov_1.252203, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000494 DBMDQJ010000000
VERSION     DBMDQJ010000494.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20332_length_1917_cov_1.252203"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /gene="thiM"
                     /locus_tag="ACZO9R_09560"
     CDS             complement(<1..543)
                     /gene="thiM"
                     /locus_tag="ACZO9R_09560"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HIR0913343.1"
                     /translation="MEAYEYLEKIRAAAPRVHCITNYVTAHDVANMILACGANPVMAD
                     EPQEMGEITAHAGALALNMGTPGERREQAMLAAGKAANANGIPVLLDPVGVGASAFRR
                     KMAEELLEAVTFTAIRGNMSEIRTLSELAEKKVTGSSGGVDVCPVDAVTEETLVETVA
                     FVKAVSKRLGAVLAVTGAIDL"
     gene            complement(847..>1917)
                     /locus_tag="ACZO9R_09565"
     CDS             complement(847..>1917)
                     /locus_tag="ACZO9R_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR0913344.1"
                     /translation="PFLVGGAIAFVINVPMKSIERHLFAKNGKLAKWRRPVAYLLTLA
                     LVIGIITLALVVVIPELGNTISMIVQQVPVAVKAVQKWLTDLPDSYPALAPAVAELNI
                     DWSSLADSIVTFVQNFAAGVVSSGVGIFSGIVSGVVTFVIAFTFSIYVLFQKERLARQ
                     AKQILYAVFPEKTTEKILSVAALSNQVFSSFLSGQCVEAVILGTLFVITMSILGMPYA
                     MLTGIVIAITALIPIFGAFIGCVIGMLLIVMVDPVQAVWFLVLFLVLQQIEGNLIYPH
                     VVGSSVGLPSIWVLVAVTVGGNMFGILGILVFIPLCSVLYALFAMFVKKRLREREIPE
                     SRWNAPSGGAAAVEPAEEQSCG"
BASE COUNT          468 a          612 c          422 g          415 t
ORIGIN      
        1 aagatcgatc gcaccggtca ccgcaagcac cgcaccaagg cgcttagaga ccgcctttac
       61 gaatgcgacc gtctccacaa gcgtctcctc cgtgaccgca tccaccggac atacatccac
      121 gccgccggat gagccggtca cctttttttc tgccagttca gaaagcgtcc ggatctccga
      181 catgttgccg cgtatggcgg taaacgtcac cgcctccaac agctcttctg ccatcttccg
      241 gcgaaatgcg ctcgcaccga cgccgaccgg atcaagcagt accggaatcc cgttcgcgtt
      301 tgccgccttt cccgccgcaa gcattgcctg ctctctgcgc tcgccaggag ttcccatatt
      361 aagggcgagc gcccccgcat gcgccgtgat ctctcccatc tcctgcggct cgtccgccat
      421 gaccgggttc gctccacacg ccaggatcat gttcgccacg tcatgcgccg tcacatagtt
      481 cgtgatacag tgtacccgcg gcgccgccgc tcttattttt tccagatatt cgtatgcttc
      541 caagtaatgc ttcccctttt tccaacgtca ttttataaat attttccact ttttagaaat
      601 ttcttacact atattaagac attcttcatt ttgccgtcaa gcttccgaca cattttcccg
      661 ataagataga atcatcaaaa ggagctgttg agtttcgacg cgggggttgg ggttctgtca
      721 gttccgccta aaagctctct caccggcact gccggcgggt tttatatata ttccatatac
      781 catattacac tatttcaaaa agcctttcgg aagtccctcc tacacttccg aaaggctttt
      841 tgtcatttat ccacaagact gctcttctgc gggctcgacc gccgcagcgc cgcctgacgg
      901 cgcgttccag cggctctcgg gaatctcccg ctcccgcagt ctttttttca caaacatggc
      961 aaacagtgcg tacagcacgg agcaaagcgg aatgaacacc agaataccca ggatgccgaa
     1021 catgttgccg ccgacggtca ctgccaccag cacccagatg gagggcagtc cgacggaact
     1081 tcccaccacg tgcgggtaga tcaggtttcc ctcaatctgc tgcaacacca aaaacagcac
     1141 cagaaaccac accgcctgca ccggatctac catcacgatc agaagcattc cgatgacaca
     1201 gccgataaat gcgccgaaaa tcggaatcag cgccgttatc gcaatcacga ttccggtcag
     1261 cattgcatac ggcatcccca gaatcgacat ggtaatgaca aacaaggtgc cgagaatcac
     1321 cgcctccacg cactgcccgg acaggaaact ggaaaacacc tgattggaca gggctgccac
     1381 cgagagaatc ttctctgtgg tcttctccgg aaagacggcg tacagaatct gttttgcctg
     1441 tctcgccaga cgctccttct ggaacagcac atagatggag aacgtaaacg caatcacaaa
     1501 cgtcaccaca ccgctcacga tcccggaaaa aataccaacg ccggagctca caacccccgc
     1561 cgcaaaattc tgcacaaacg tcacaatcga gtcggcaagc gatgaccagt caatattaag
     1621 ctccgccacc gcaggggcaa gtgccggata gctgtccgga agatctgtca gccacttctg
     1681 tacggctttt accgcaaccg gaacctgctg tacaatcatg gaaatcgtat ttccaagctc
     1741 cggtatcacg accacaagtg ccagcgtgat aataccgatc acaagcgcaa gcgtcagcag
     1801 ataggcaacg ggtcttctcc attttgcaag cttcccgttc ttcgcaaaca gatgccgctc
     1861 gatactcttc atcggaacat tgatcacgaa tgcgatcgcg cctccaacca gaaacgg
//
LOCUS       DBMDQJ010000495         1909 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20448_length_1909_cov_1.424226, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000495 DBMDQJ010000000
VERSION     DBMDQJ010000495.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20448_length_1909_cov_1.424226"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(319..906)
                     /locus_tag="ACZO9R_09570"
     CDS             complement(319..906)
                     /locus_tag="ACZO9R_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HIR0913345.1"
                     /translation="MDFSNYNIGLGITGSFCTFAKARKEIQRLCDMGANVVPVFSFNA
                     QTCDTRFGSAKDYVDGICEITGNEGIRTICAAEPIGPNNFLDIMVIAPCTGNTAAKLC
                     NGITDTPVLMATKAHMRNGKPLVIAISTNDALGASFKNIGMLMNMKNIYFVPFGQDNC
                     KSKPNSMIAKMELLPDTIEAALAGRQIQPILQTPT"
     gene            complement(906..1748)
                     /locus_tag="ACZO9R_09575"
     CDS             complement(906..1748)
                     /locus_tag="ACZO9R_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HIR0913346.1"
                     /translation="MNRQTILFISDMRQVYLAEIMTKRGLNVRCLDIRNSGTVKEQLE
                     KLKSFLAEADMLILPIPVTKIPEQKMLNDILNKNLTNDTLVLGGCFSPEQQELLERRD
                     IHYLDFMKDEIVTEENAVATAEGVIAELVNHSPYNIEEAKIIVTGYGCCGRAVAARLK
                     ALGARVTVLARRREVRKLAKKDGFYAADFAFGPEEAMGAAMLVNTVPAPVVTGAIIRE
                     LPRDAYILDIASKPGGTDFACARECGIRADLALGLPGKYAPKESAYILDRAVERFVRK
                     EHEM"
BASE COUNT          427 a          540 c          411 g          531 t
ORIGIN      
        1 ctactattga aaacagggca gatgccaact cagacatcgg cttcgcgcgg ttatttttgt
       61 tcattggtga tgcaaggaac gcacaaaagt ccttaaattg tctgccggct tgcgaacaca
      121 cccgcggagt gctcaaagct tggaaagaca cggatcatcg aagatgccga atgcggtgcg
      181 gattagaaat gccgggtttt tcgattagaa tcccccttgc caggagcatc cggaactcta
      241 aaatgatcta tgatctacta tataaaacgt ccccggcatt ttccgtctct gtctccgccc
      301 ggctccgtca gatctgcgtt acgtcggggt ctgcagaatc ggctggatct gcctgccggc
      361 gagcgccgcc tcgattgtgt ccggcaggag ctccattttt gcgatcatgg agttcggctt
      421 gctcttgcag ttgtcctgcc cgaacggaac gaaataaata tttttcatat tcatcagcat
      481 tccaatattc ttaaagctgg cgccgagcgc atcgttcgtc gagatggcaa tcacaagcgg
      541 cttaccgttg cgcatatgcg ctttggtcgc catcagtacc ggcgtatccg tgattccgtt
      601 gcagagtttt gccgccgtat ttccggtgca gggcgcaatg accatgatgt ccagaaaatt
      661 attcggtccg atcggctccg cagcacagat ggtacggatg ccctcatttc cggtaatctc
      721 gcagattccg tccacataat ctttcgcact tccgaaccgc gtgtcacacg tctgcgcatt
      781 aaatgaaaaa accggcacca cattggcgcc catatcgcac agccgctgta tttcttttct
      841 tgctttcgca aacgtacaaa acgaaccggt aattccaagc ccgatattat aattggaaaa
      901 atccatcaca tttcatgctc ctttcgcacg aatctctcca ccgcccggtc cagaatatag
      961 gcgctctcct tcggcgcata ttttcccgga agtccaagtg ccagatccgc acggatgccg
     1021 cactctctgg cgcaggcgaa atccgtaccg cccggtttgg acgcgatgtc taaaatatat
     1081 gcatcccgcg gcagttcgcg gatgatcgca cctgtaacca caggtgccgg aaccgtgttg
     1141 accagcattg ccgcccccat cgcttcctcc ggtccaaacg caaaatccgc ggcataaaat
     1201 ccatccttct tggcaagttt acgcacttcc cgtcttcttg ccagcactgt cacgcgcgcg
     1261 ccgagcgctt tcagcctcgc agccaccgct ctgccgcagc agccgtaacc ggtgacgata
     1321 atttttgcct cctcaatgtt ataagggctg tggttcacaa gctccgcgat cacgccctcc
     1381 gccgtcgcca ctgcattttc ttccgtcaca atctcatctt tcataaaatc cagatagtga
     1441 atgtccctgc gctccaaaag ttcctgctgt tccggcgaga agcagcctcc aagcaccagc
     1501 gtatcgtttg tcaggttttt attcaatata tcgttcagca ttttctgttc cggtatcttt
     1561 gtcaccggaa tcgggaggat cagcatatcc gcctccgcca gaaaactttt caacttctcg
     1621 agctgttctt ttaccgtccc gctgtttctg atatccaggc agcgtacgtt gagtcctctt
     1681 ttggtcataa tctccgccag atagacctga cgcatgtcac tgatgaaaag tatggtctgt
     1741 ctattcatag aattacgctc ccttctcttt attatatgaa acgcgcgctc ttctgccaca
     1801 gcgaagcatt tacatgtatt ttacgaaaaa tatatattat atctattgca tctatatttt
     1861 cagaatattt tttcgtttat tctttgttta ttgtttttaa atatttttt
//
LOCUS       DBMDQJ010000496         1908 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20466_length_1908_cov_1.225235, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000496 DBMDQJ010000000
VERSION     DBMDQJ010000496.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1908)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20466_length_1908_cov_1.225235"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="ACZO9R_09580"
     CDS             complement(<1..938)
                     /locus_tag="ACZO9R_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HIR0913347.1"
                     /translation="MLKRLGKTITNNFSLKILAVILAVVLWVVVINIDDPTTSKTYTT
                     NVVAENTDYITSQNKYYEPLDSSNVVSFRVSAARSVHDELSNADFSATADMENIEYDE
                     GSGIYRVPVTITAKRYSNKVSVVSKQLYLEVALEDRGTCQKAITAATKGTVMDGCALG
                     TVQIVGSNLLKVSGPASIVSQIDTAVATINVEGMSTDVTDSVVPVLYDADGNVIDTTK
                     LTLSLSTVNISAQILNTKDVPLEFEAKGEPADGYVLTGVESSLDNVRIKGEAATLNTV
                     SKITIPQEVLDISGITEDLTTTVDISSYLPSQTALVL"
     gene            complement(904..1800)
                     /gene="cdaA"
                     /locus_tag="ACZO9R_09585"
     CDS             complement(904..1800)
                     /gene="cdaA"
                     /locus_tag="ACZO9R_09585"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HIR0913348.1"
                     /translation="MLATLDAFWQKASLYMNLPKISITDIVEIIIIAFLIYYMLVWIK
                     NTRAWMLLRGLMVILLFVLLAALFQMNTIIWIARNTLNVLVIALVVIFQPEMRKALEN
                     LGRKNVLAFLFSFDSSKEDTGKFSDKTINEMVKACYEMGKVKTGALIVVEDEIVLSEY
                     ERTGIAVDGILTSQLLINIFEKNTPLHDGAVIVRGDRVVAATCYLPLTDSLSISKDLG
                     TRHRAAVGISEVSDSLTIVVSEETGRVSIALGGELYRNVDAEFLKNKLTYIQRREREL
                     SKIALWKRRLKDAKTARKNDNK"
BASE COUNT          433 a          554 c          423 g          498 t
ORIGIN      
        1 agcacaagtg ccgtttgact cggcagatag gaactgatat ccaccgttgt ggtcagatcc
       61 tcggtgatac cggaaatgtc cagcacctcc tgcggaatgg tgatcttgct gacggtattg
      121 agcgttgccg cctcgccctt gatacgcaca ttatccaggc tgctttccac gccggtaagc
      181 acatagccgt ccgccggttc gcccttcgcc tcaaactcca acggcacatc cttggtattt
      241 aagatctgtg ccgaaatatt gactgtggac agactgagtg tcagctttgt cgtgtcgatc
      301 acgttgccgt cggcatcata taacaccggc acaacggaat ccgtcacatc cgtggacata
      361 ccttccacat tgatcgtcgc caccgccgta tcaatctggc tgacgatgga tgccggtccc
      421 gaaaccttta acaggttgga gcccacgatc tgtaccgtgc cgagtgcaca gccgtccatc
      481 acggttccct tggttgccgc cgtaatcgcc ttctggcagg tgccgcggtc ttccagcgcc
      541 acttccagat agagctgttt ggagacgacg gagactttat tgctataccg cttcgcggtg
      601 atcgtcaccg gcacgcggta aattccgctt ccctcatcgt actcaatatt ctccatgtcc
      661 gccgttgcgg aaaaatcggc attggataat tcatcgtgca cgctgcgcgc agccgacacg
      721 cggaatgaca ctacgttgct gctgtccagc ggttcataat atttgttctg cgatgtgata
      781 tagtccgtat tctccgctac aacattggtg gtataggtct ttgacgtcgt cggatcgtca
      841 atgttaatca cgactaccca cagcacaacc gccagtatga ccgccagtat tttgaggcta
      901 aaattatttg ttatcgtttt tccgagccgt tttagcatct ttcagcctcc tcttccacaa
      961 tgcaatcttc gataattccc gttcccgcct ctggatgtac gtcagcttgt tcttcagaaa
     1021 ctcggcatct acattgcggt aaagttcacc gccgagcgca atcgacactc ttccggtctc
     1081 ctctgacaca acgattgtca gggaatcgct cacctcgctg attccgacag ccgcacggtg
     1141 tctcgttccc agatccttgc tgatcgacag ggaatccgta agcggcaggt aacaggtcgc
     1201 cgccacgact ctgtccccgc gcacaatcac ggcgccgtca tggagcggtg tgtttttttc
     1261 aaaaatattg atcagaagct ggcttgtcag aataccgtcc accgcgattc cggtgcgctc
     1321 atattccgag agaacaatct catcctccac cacaatcagg gctccggtct tcacctttcc
     1381 catctcgtag cacgccttga ccatttcatt aatggtctta tccgagaact tccccgtatc
     1441 ctcctttgag gaatcaaagg aaaacaggaa cgccagaacg tttttccgtc cgagattctc
     1501 aagcgccttg cgcatctccg gctggaagat tacaaccaga gctatcacca ggacgtttaa
     1561 cgtattcctt gcaatccaga tgatcgtgtt catctggaac aatgctgcca acagaacgaa
     1621 gagcagaatc accatcaaac cgcgcagcag catccatgct ctggtattct tgatccacac
     1681 cagcatatag tagatcagaa acgctatgat tatgatctcc acgatatctg ttatggagat
     1741 ttttggaaga ttcatataaa gagacgcttt ctgccagaaa gcatcgaggg tagctaacat
     1801 gttctgcacg cggtccactt ccttaatcta ataattcttc gtcgatatcc atctcttccg
     1861 caaaacgctt cttcgtgaga cgttcctttt cgtccttccc gctgaaac
//
LOCUS       DBMDQJ010000497         1897 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20602_length_1897_cov_1.293430, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000497 DBMDQJ010000000
VERSION     DBMDQJ010000497.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20602_length_1897_cov_1.293430"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /gene="cas1"
                     /locus_tag="ACZO9R_09590"
     CDS             complement(<1..422)
                     /gene="cas1"
                     /locus_tag="ACZO9R_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HIR0913349.1"
                     /translation="MRQLLNTLFVTSEDIYLSLDGENVVANRGGETVARYPLHTLQSI
                     VTFSYAGASPALMGKCAQREIGLAFCSQRGKFLARVSGQMQGNVLLRRMQYRVADDPS
                     QSCRIARNMIFGKVYNARWSVERTRRDHAPRVDGQRFSA"
     gene            complement(419..1081)
                     /gene="cas4"
                     /locus_tag="ACZO9R_09595"
     CDS             complement(419..1081)
                     /gene="cas4"
                     /locus_tag="ACZO9R_09595"
                     /EC_number="3.1.12.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007489327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HIR0913350.1"
                     /translation="MDFNEDDFLQLSGLQHFKFCRRQWALIHVENQWAENFRTTDGAI
                     LHENAHNGGFFESRGDLLITRDMRIFSRTLGVSGACDVLEFHRGKIGIPLKGREGLWQ
                     PYPVEYKRGRSKENTADALQLCGQAMCLEEMLCCEIPQGALYYGEPHRRTEVDFTPEL
                     RQEVTDLLTEMHELYRRGYTPKVKPSKSCNACSLKELCLPKLLRSRSVSAYLRGAMED
                     EP"
     gene            complement(1071..>1897)
                     /gene="cas7c"
                     /locus_tag="ACZO9R_09600"
     CDS             complement(1071..>1897)
                     /gene="cas7c"
                     /locus_tag="ACZO9R_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas7/Csd2"
                     /protein_id="HIR0913351.1"
                     /translation="NKIDFVALISVNMANANGDPLNGNRPRTDYNNYGEISDVCIKRK
                     IRNRMQDQGYPIFVQSEDRCDDGLGSLSERASTTLKGIKNREEYTKKACETWLDVRAF
                     GQVFAFKSKDSVSAGVRGPVSIHQAVSISPVDVQTLQITKSVNGDPGEKRSSDTMGNK
                     HFVQFGLYLLKGSINVQLAEKTGFTEEDAQVVKECLRTLFVNDASAARPDGSMEVVKL
                     FWWQHNCEDGQYSSAKVHRSVKVTLKDSDQIPSSVDDYAITVEALPGLEPEVIDGL"
BASE COUNT          431 a          552 c          477 g          437 t
ORIGIN      
        1 gcggaaaacc gctgaccatc gacacgcgga gcatgatccc gccgggtacg ctccacgctc
       61 catcgggcat tgtagacctt tccgaaaatc atattccggg ctatgcgaca gctttgcgat
      121 gggtcatccg ccacgcggta ctgcatgcgc cgcagcagga catttccctg catctgcccg
      181 gacacgcggg caaggaactt ccctctctgc gagcagaacg ccagtccgat ctcccgctgc
      241 gcacattttc ccatcagggc gggtgacgct cccgcataag aaaaggtgac gatgctttgc
      301 agcgtgtgca acggataccg ggcaacggtt tccccgcccc ggttcgccac cacattttca
      361 ccatccaagg agaggtagat atcctccgag gtgacaaaaa gcgtattcaa tagctgtctc
      421 atggctcgtc ctccatggcg ccgcgcaaat acgcggaaac agatctgctc cgcagcagct
      481 ttggcagaca cagttccttc agggaacagg cattgcagga ctttgacggc ttcaccttag
      541 gcgtgtagcc gcgccggtaa agctcgtgca tttcggttaa gagatccgtt acctcctgac
      601 gcagctccgg tgtaaaatct acctcagtgc ggcggtgagg ctcgccgtaa tacagcgcac
      661 cctgcggaat ttcgcagcag agcatttcct ccagacacat agcctgtcca cagagttgga
      721 gggcatctgc cgtgttctcc ttcgaccgtc cccgcttgta ctccacgggg tatggctgcc
      781 acagcccctc ccggcctttg agcggaatgc cgatcttgcc ccggtgaaac tccaacacat
      841 cgcaggcacc ggacacaccc agcgtccggg aaaaaatacg catatcacgg gtgatgagga
      901 ggtctccccg gctctcgaaa aacccaccgt tgtgggcgtt ttcgtggagg atcgccccgt
      961 cggtggtgcg gaaattttcc gcccactgat tctcaacatg gatgagcgcc cactggcggc
     1021 ggcagaactt gaagtgctgc aaaccggaga gttgaaggaa atcatcctca ttaaagtcca
     1081 tcaatcactt ccggttccaa tccaggcagc gcttctacag tgatggcata gtcatccaca
     1141 gaggatggaa tctggtctga atctttcaga gtgactttta cagaacggtg tactttggcg
     1201 gaagaatact gcccatcctc gcagttatgc tgccaccaga aaagcttgac cacctccata
     1261 gaaccatctg gacgggcggc ggaagcatca ttgacaaaca gcgtccgtag acactccttc
     1321 accacttgtg cgtcctcttc tgtgaagcct gttttttctg ccaactgcac attgatggag
     1381 cctttgagaa gataaaggcc gaattgcaca aaatgcttat tgcccatagt atctgaagaa
     1441 cgtttttcac cggggtcacc gttgacactt tttgtaatct gaagcgtctg cacatccacg
     1501 ggtgaaatac tgaccgcctg atgaatggaa acggggccac gcacacctgc agatacactg
     1561 tctttgctct taaacgcgaa cacttgtccg aatgcgcgca catccagcca agtttcgcag
     1621 gcttttttgg tatactcctc acgattttta atgcctttca gggttgtgga agcgcgttcg
     1681 ctcaggctgc caaggccatc gtcacaccgg tcctcagact gcacaaaaat cggatagcct
     1741 tgatcctgca tccggttgcg gatttttcgc ttgatgcaca catcggagat ctcaccataa
     1801 ttgttataat ccgtgcgggg ccggttgccg tttaaggggt caccgttggc atttgccatg
     1861 tttactgaga tcaatgccac aaaatcgatc ttattct
//
LOCUS       DBMDQJ010000498         1897 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20603_length_1897_cov_1.228285, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000498 DBMDQJ010000000
VERSION     DBMDQJ010000498.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20603_length_1897_cov_1.228285"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="ACZO9R_09605"
     CDS             complement(<1..1444)
                     /locus_tag="ACZO9R_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HIR0913352.1"
                     /translation="MKDFIKINENDNVIVALKELTAGETILAGTKEVTARETIPAGHK
                     MAIRDIAAGAEVIKYGFRIGNAKENIAAGQWVHVHNLKTALGDLLEYTYQPVETNVKK
                     TEDVKFMGFARANGKVGVRNEVWIIPTVGCVNNIATAMAKAANARVKGSVEEVIAFPH
                     PYGCSQMGDDQEHTRTILADLVNHPNAGGVLVLGLGCENSNIGKLKKYIGDYDENRVK
                     FLVCQECEDEMEEGAKLLNELIDYASAFEREPISASKLIVGMKCGGSDGFSGITANPL
                     VGRFSDLLIGKGGTTILTEVPEMFGAETLLMNRCANRELFDEAVSLINDFKQYFKDNH
                     QTIYENPSPGNKKGGISTLEDKSLGCTQKSGSAPVCGVLSYGECVKTSGLNLLSAPGN
                     DLVAATALAASGAHIVLFTTGRGTPFASPVPTVKISSNSTLAGKKKNWIDFNAGVLVE
                     DAELDTVGQQLFDYVIDVASGRKVCSEEAGF"
     gene            complement(1476..>1897)
                     /locus_tag="ACZO9R_09610"
     CDS             complement(1476..>1897)
                     /locus_tag="ACZO9R_09610"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079794.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HIR0913353.1"
                     /translation="PYVKHALLSISLNSVSKWRARCMPSFLGYIEKEGKLPVHLTFSL
                     AALMAFYTGTEIRDKALIGHRDGQEYNILDDAAVLEFFAANSSKGAREYAHAVLSNEA
                     FWGQDLSALAGVEDAVASYIEEIRALGMRKAMEKIFA"
BASE COUNT          388 a          568 c          428 g          513 t
ORIGIN      
        1 ggaagcccgc ttcctcgctg cagacttttc tgccggacgc cacgtcgatc acatagtcga
       61 acagctgctg ccccactgtg tctaactccg cgtcttccac cagtacgccc gcattgaagt
      121 cgatccagtt tttcttcttt ccggccagcg tactgttgct ggaaatcttc acggtcggaa
      181 ccggggacgc aaacggtgtt ccgcgccctg tcgtaaacag tacgatatgt gcgccgctcg
      241 ccgcaagcgc cgtcgccgcc accagatcat tgcccggtgc agacaggagg tttaatcctg
      301 aggtttttac acactctcca taggaaagca cgccgcacac cggtgcgctt ccggacttct
      361 gtgtacagcc cagtgattta tcttccagtg tcgagatacc acccttctta tttccaggag
      421 acggattctc ataaatcgtc tgatgattgt ccttgaaata ctgcttgaag tcgttgatca
      481 gtgagactgc ctcatcgaac agttccctgt tggcgcagcg gttcatcaga agcgtctccg
      541 cgccaaacat ttccggcact tcggtaagga tcgtggtgcc gccctttccg atcagaagat
      601 ctgagaatct gcccaccagc ggattggccg taattccgga gaatccatcg ctgccgccgc
      661 acttcattcc gacaatcagc tttgaggcac tgatcggctc gcgctcaaac gcggacgcat
      721 aatcgatgag ttcattcagg agttttgctc cctcctccat ctcgtcctca cattcctgac
      781 aaaccaggaa ttttacgcga ttctcatcgt aatcgccgat gtattttttc aatttgccta
      841 tattgctgtt ctcacatccc agtccgagca cgagaacgcc gcccgcattc ggatgattta
      901 ccagatccgc cagaatcgtt ctggtgtgct cctggtcgtc tcccatctgg gaacatccat
      961 acggatgcgg gaatgcgatg acttcctcaa cgcttccctt cactctggcg ttggctgcct
     1021 tcgccattgc ggttgcaata ttgttgacac agccgacggt cgggatgatc cagacttcgt
     1081 tgcggacgcc aacttttccg ttggcgcggg cgaagcccat aaatttgaca tcctctgtct
     1141 ttttcacatt cgtctccacc ggctgataag tgtattccag cagatcgccg agtgccgtct
     1201 ttaaattatg tacatgcacc cactgtcctg ctgcaatgtt ctccttcgca ttgccgatcc
     1261 taaaaccgta tttaatcacc tcggctccgg ccgcaatatc gcggattgcc atcttgtgtc
     1321 ccgccgggat cgtttccctg gcggtaacct ccttcgttcc cgccagaatg gtctccccgg
     1381 ctgtcagttc cttcaatgcc acgataacat tatcattttc attgatcttg ataaaatcct
     1441 tcatgaatcg tctcctgcct gtttttcaca gtgtattatg caaaaatctt ctccattgcc
     1501 ttacgcatcc caagtgcgcg gatctcttct atataggaag caactgcatc ctccactcct
     1561 gcaagagcgc tcagatcctg tccccagaat gcctcgttgg agagaactgc atgtgcatac
     1621 tctctcgcgc ccttagaact gttggctgcg aagaactcca ataccgcagc gtcgtctaaa
     1681 atattgtact cctgtccgtc gcggtgtccg atcagcgcct tgtcgcggat ctctgttcct
     1741 gtatagaatg ccatcagggc tgccagcgag aatgtcaggt gtaccggaag ctttccttcc
     1801 ttctcgatgt accccaagaa gctcggcata catcttgctc tccatttgga tacagagttc
     1861 agggagatgg ataacagtgc gtgctttaca tacgggt
//
LOCUS       DBMDQJ010000499         1895 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20641_length_1895_cov_1.365663, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000499 DBMDQJ010000000
VERSION     DBMDQJ010000499.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1895)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20641_length_1895_cov_1.365663"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="ACZO9R_09615"
     CDS             complement(<1..328)
                     /locus_tag="ACZO9R_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0913354.1"
                     /translation="MEKEEKKDITIADVAEALGVSKTTVSRAISGKGKIGKETRKRVL
                     EYIEEHDYKPNAIAKGLAQSKTYNICVVMPRDYEVVDWNFFQRCLFGIQEMAETVGYD
                     ILLTMCQ"
     gene            613..>1895
                     /locus_tag="ACZO9R_09620"
     CDS             613..>1895
                     /locus_tag="ACZO9R_09620"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HIR0913355.1"
                     /translation="MRTDHLLFGAAYYLEYLPYDRLETDMEMMERAGMNTIRIAESTW
                     STLEPSDGVFDFTCIDRMVQAAAKHHFSVIIGTPTYAIPTWLARKYPDILAVTANGQE
                     LYGHRQNMDITHPGYRYHAERIIRAMMEHLKDVPNIIGFQLDNETKSYGTAGPRVQQM
                     FVASLKKKYPDILDFNREFGLDYWSNRVNDWADFPDVRGTINQSLAAEFQRFQRSLVT
                     EFLSWQSDIIKEYKRDDQFITQNFDFDWTDHSYGYQPEVDQYDAARCMTVAGADIYHP
                     SQDDLTGAEITVCGNIARSLKKDNYLILETEAQGLTPWLPYPGQLRLQAYSHLANGSN
                     SVMYWHWHSIHNAIESYWKGVLSHDFSENATYREASEIGAEWKRIGTHLMNLKKENRV
                     AILLDNLSLAGLRQFPLETTGDHSYNAVMRWLADAL"
BASE COUNT          458 a          544 c          439 g          454 t
ORIGIN      
        1 tctgacacat cgtaagcaga atgtcatatc cgaccgtctc cgccatctcc tgaataccga
       61 acaggcagcg ctggaagaaa ttccagtcca ccacttcata atcgcgcggc atcaccacac
      121 agatattgta cgttttcgac tgtgccagtc ccttggcgat ggcgttcggc ttataatcgt
      181 gctcctctat gtattccaga accctcttgc gggtctcttt cccgattttt ccctttccgg
      241 atatggcgcg ggaaaccgtt gtctttgaaa cccctaacgc ctccgctaca tccgcaatgg
      301 taatatcttt tttctcttcc ttctccatag taacctccaa ccccggacca tcgtccggag
      361 ttccccttta ttttgcgctt gttgcgcaat ctttttttat tatcaaacac aattccggaa
      421 aaatcaatcc ccttttttac caaaaataag ttctcatttt ttgcagtatt tacggtactt
      481 tctgttgttt acagtccatt tttgtgatgg tataatgaaa aagcgcaaac ggttgcacgt
      541 ttttgtgtgc cgaaatgcgc aaaacagcta cttcttatag aaaattttag aaaagattgg
      601 agatcacccg atatgagaac agatcacctg ctctttggag cagcctacta tctggaatat
      661 ttgccgtatg accgtctgga gacagatatg gaaatgatgg agcgtgccgg catgaatacc
      721 atccgcatcg ccgaatccac ctggagcacc ttagaaccat ccgacggcgt ctttgacttt
      781 acctgcatcg accgcatggt acaggccgcc gcaaagcatc atttttccgt catcatcgga
      841 accccgactt acgcgatacc aacctggctg gcgcgcaaat acccggatat tctcgccgtc
      901 acagcaaacg ggcaggagct ctacggtcac cgtcagaaca tggacatcac gcaccccggc
      961 taccgttatc acgccgagcg catcatccgc gccatgatgg agcacttaaa ggacgttccg
     1021 aacatcatcg gcttccagct ggacaacgag accaagagct acggaacggc cggtccgcgg
     1081 gttcagcaga tgttcgtcgc ttccctgaag aaaaaatacc cggacatttt ggatttcaac
     1141 cgtgaattcg gtctggatta ctggagcaac cgcgtgaacg actgggcgga ttttccggat
     1201 gtacgcggca cgatcaacca gagtcttgcc gcagaatttc agcgttttca gcgctccctc
     1261 gtcacagagt tcctctcctg gcaatcggac attatcaaag agtacaaacg tgacgaccag
     1321 ttcatcacgc agaattttga ttttgactgg accgatcact cttacggcta tcagccggag
     1381 gtcgaccagt atgacgccgc ccgctgcatg accgtggcag gcgccgatat ttaccatcct
     1441 tcgcaggacg acctgaccgg agctgagatc actgtatgcg gaaatattgc gcgcagctta
     1501 aagaaggaca attatctgat cttagagaca gaggcgcagg gtctgacccc gtggctcccc
     1561 tatccggggc agctgcgcct gcaggcgtac agccaccttg caaatggctc caacagcgtg
     1621 atgtactggc actggcactc gatccacaat gcgatcgaaa gctactggaa aggcgtgctt
     1681 tcccacgatt tttccgaaaa tgccacctac cgggaagcca gcgaaatcgg cgcggagtgg
     1741 aagcgcatcg gcacacacct catgaatctg aaaaaggaaa accgcgtcgc catcctgctg
     1801 gacaatctct ccctggcggg gctgcgccag tttccgcttg agaccaccgg tgaccacagc
     1861 tacaatgccg tgatgcgctg gcttgccgac gccct
//
LOCUS       DBMDQJ010000500         1894 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20659_length_1894_cov_1.075293, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000500 DBMDQJ010000000
VERSION     DBMDQJ010000500.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20659_length_1894_cov_1.075293"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..860)
                     /locus_tag="ACZO9R_09625"
     CDS             complement(<1..860)
                     /locus_tag="ACZO9R_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005612977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HIR0913356.1"
                     /translation="MSEKIASINSVVNSFIWGVPAMICIIGVGILLTVRTRCIQVRKF
                     GVAMKNTIGKIFDKTQARDGSMSPFQAVCTALAGTVGTGNIAGVAGAIALGGPGAIFW
                     MWCSAFLGMCTKFSEVTLAIHFREKNKNGEYVGGPMYYIKNGLSKKWHFLAVFYAVFG
                     VLTVFGTGNATQVNTIVSSVNTALMNFNILKGEPNSNVNLIFGIFIAALVAMVLLGGI
                     KRIGQVSEKLVPFMAVLYVILALGVIILNIQRVPGVFAQIVSGAFTPRAATGGIIGSM
                     FLSMKKGVSRG"
     gene            1060..1539
                     /locus_tag="ACZO9R_09630"
     CDS             1060..1539
                     /locus_tag="ACZO9R_09630"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015522087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR0913357.1"
                     /translation="MAYYVAEDAVIKGNITIGEDSGIWYNATIRGDSNQIAIGSRTNV
                     QDNAVVHVSSGYPVSIGDDVTVGHGAIVHGCTVGSNTLIGMGAIILNGAVIGDNCIIG
                     AGALVTQNTHIADGSLAFGNPAKVIRSLTPEEIRETGNNAQRYVNAAKAQLREVNCE"
     gene            1715..>1894
                     /locus_tag="ACZO9R_09635"
     CDS             1715..>1894
                     /locus_tag="ACZO9R_09635"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004606504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR0913358.1"
                     /translation="MLLVIDVGNTNITMGVYDGKNLKTTFRMMSKQPRTSDEYGITLR
                     ELLRTNEVEKEEIEGV"
BASE COUNT          560 a          389 c          481 g          464 t
ORIGIN      
        1 cctcttgaaa cacccttttt catactaagg aacatcgatc cgatgatacc gccggttgct
       61 gctctggggg taaaggcacc actcacaatt tgggcaaaga ctccgggtac gcgttggatg
      121 ttcaggataa ttacgcccaa ggcaaggatg acatacagaa ctgccataaa agggacgagc
      181 ttttcagata cctgtccgat tcgcttgatt ccgccaagca gtaccatggc aaccagtgcg
      241 gcgataaaga tgccgaagat caggttaaca ttgctgttgg gttcgccctt taaaatatta
      301 aaattcataa gtgcggtatt tacagaagaa acaatggtat ttacctgagt cgcattaccg
      361 gttccaaaga cagtcagtac accgaagacc gcataaaaga cagccaggaa atgccatttt
      421 ttagacaggc cgtttttaat gtaatacata ggtccgccaa cgtattcgcc gtttttattt
      481 ttttcacgga aatggatggc gagggttact tcagaaaact tggtacacat gcctaagaat
      541 gcggaacacc acatccagaa gatggcaccc ggtccgccaa gtgcgatggc accggcaacg
      601 cctgcgatat ttccggttcc gacggttccg gcaagggccg tacatacagc ctgaaaggga
      661 ctcatggagc catccctggc ctgcgttttg tcaaaaatct ttccgatggt gtttttcatg
      721 gcaacgccaa atttgcgcac ctgaatacat ctcgttctga cggttaaaag gatgccaacg
      781 ccgatgatgc agatcatggc gggaacaccc cagatgaagc tgtttacaac actgttaata
      841 cttgcaattt tttctgacat tttcccttta ttccctcgta aattttttaa aacgacaact
      901 taattattat gacatagaat cgtgtcggtt tacaagaaat tttttgagga taattggcgg
      961 atggataaca ggcaatagaa aattaacaat gtgaattgag atctgttcca gtccgtgata
     1021 cgataagaaa gagatcagat tggatcagga ggacaaacca tggcttacta tgtggcagag
     1081 gatgcagtta tcaaaggaaa tatcacaatt ggagaagact cgggcatctg gtacaatgca
     1141 acgatccgtg gcgattccaa tcagattgcc attggcagca ggacgaatgt acaggacaat
     1201 gccgtggtgc atgtgagctc cggctatccg gtgagcattg gggatgatgt gacggtcgga
     1261 catggagcga ttgttcatgg atgtacggtt ggaagcaaca cactcattgg aatgggagct
     1321 attattctga acggagcagt gattggagac aactgcatca ttggtgcggg agctcttgtg
     1381 acacagaata cacacattgc ggatggaagc cttgcctttg gcaatccggc gaaagtgatc
     1441 cgcagcctga ctccggagga aatcagggaa acggggaata atgcacagcg atatgtgaat
     1501 gcggcgaagg ctcagctccg ggaagtgaac tgtgaataat tactgtaatt gttgcaacta
     1561 ttcagtacag gtcgaagcat ttactgctga gaccgtaaga aaatgattca gtaatgcaaa
     1621 atcccatcgg cgaagccgat ttggaatgga ttttgcatcg taactgtgaa agtgctgaac
     1681 agttacgaaa tgtgtgaata ggaaaggaaa gaatatgctg ctggtcattg atgttggcaa
     1741 tacaaatatt acaatgggcg tttatgacgg aaagaattta aagaccacct tccggatgat
     1801 gagcaaacag cccagaacct ccgatgagta tggaattacg ctgcgcgagc tgcttcgtac
     1861 gaatgaggtg gaaaaggaag aaatcgaggg agtc
//
LOCUS       DBMDQJ010000501         1893 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20663_length_1893_cov_1.910714, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000501 DBMDQJ010000000
VERSION     DBMDQJ010000501.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20663_length_1893_cov_1.910714"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            102..713
                     /locus_tag="ACZO9R_09640"
     CDS             102..713
                     /locus_tag="ACZO9R_09640"
                     /EC_number="2.3.1.275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR0913359.1"
                     /translation="MSSYLGFVIGGYLLGSILFAYVLPKLTRHIDIREMSEDGNPGTY
                     NAFRYGGTVCGICVLLLELLKGFFPVWLCERTVGLQSPWFAAVMAAPVLGHAYSVYHR
                     GRGGKAIAVSFGVLLGLVPAGIWQPLAMLVLFYLLFSLLLKIKSHARRSVVTYLCFAA
                     GTCLTVKERPVLMGSLLIAGTVIYRHTFGCLGEEKNERTDFIM"
     gene            688..1866
                     /locus_tag="ACZO9R_09645"
     CDS             688..1866
                     /locus_tag="ACZO9R_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009247 - glycolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGDG synthase family glycosyltransferase"
                     /protein_id="HIR0913360.1"
                     /translation="MNVLILSCNTGGGHNAAARAVAEVFRAHGDEAVVLDYLKLAGEK
                     VSRTVGNVYVETVKIAPGLFGLLYKIGMLVSRLLRRSPVYYVNGKMAVYLAAYLKKHP
                     ADVIVMPHLYPAETITYMKRKGMKLPLTVAVMTDYTCIPFWEETDCDYYMIPHESLIP
                     EIVKRGIPEEKLVVTGIPVAAACSEPWSGMPEESAAADTHTHSIARKNAQQAAKKSLG
                     LEEKQRYILVAGGSMGAGSIDRLIPALLRRMQREEHLILICGSNQKLEQRMRARFGRD
                     ARVTILGSVSNMPVYLHACDIIYTKPGGLTSTEAAVTEIPIVHSKPIPGCETKNRSFF
                     VKKGMSVTAYTEYGLVRKGMQLLHNSGKKNAMQRAQCQGIEKHSAEKIYQFVREKGIY
                     "
BASE COUNT          441 a          420 c          597 g          435 t
ORIGIN      
        1 gggtgacaga aagagtttct gccgccctgt cttttcttat gacagaaaat atgttacatt
       61 acatatcagt gttagaaacg agagcgatag gagcagcggt tatgagcagt tatctgggat
      121 ttgtgatcgg cggctatctt ctgggaagca ttttatttgc ttacgtgctt ccgaagctga
      181 cccgccatat cgatatccgg gagatgtcgg aggacggcaa tcccgggacc tacaatgcgt
      241 tccggtacgg cggcacagtc tgtggaatct gtgtgttgct gctggagctt ttgaagggat
      301 ttttcccggt atggctgtgc gagcggactg tgggtctgca atccccgtgg tttgcggcgg
      361 taatggcggc accagtgctg ggacatgcct attccgtcta tcaccgcggg cgcggcggca
      421 aggcgatcgc ggtatccttc ggggttctct taggactggt gccggcggga atctggcagc
      481 cgctagcgat gctggtgctg ttttatctgc tgttttcact tttgcttaag atcaaaagcc
      541 atgcgaggcg gagcgttgtg acgtatctgt gttttgcagc gggaacctgt cttacggtga
      601 aggagcggcc ggttctgatg ggaagcctgc tgattgccgg aacggttatc taccggcaca
      661 cattcggatg cctgggggag gaaaaaaatg aacgtactga ttttatcatg taatacaggc
      721 ggcggtcaca acgcggcggc acgtgcggtt gcggaggttt tccgtgcgca cggggacgag
      781 gcggtggtgc tggattatct caagcttgcg ggagagaagg tctcgaggac ggtcggtaac
      841 gtctatgtgg agacagtaaa gattgccccg ggtctgttcg gacttttgta taagatcggc
      901 atgcttgtca gccggcttct ccgccggtcg ccggtctatt atgtcaacgg gaagatggca
      961 gtctacctgg cggcttatct gaagaaacat ccggcggacg tgattgtcat gccgcatctg
     1021 tatccggcgg aaaccattac ctatatgaag cgaaagggaa tgaagcttcc gctcacggtg
     1081 gcggtgatga cggattatac ctgtatcccg ttctgggaag agacggactg cgactattat
     1141 atgattccgc acgagagtct gatcccggag attgtgaagc gtgggattcc ggaagaaaag
     1201 ctggtggtaa cgggcattcc ggtggcggcg gcatgcagtg agccgtggag cggtatgccg
     1261 gaagaaagtg ctgcggcgga tacacacacg cacagcattg cgcgcaaaaa cgcgcagcag
     1321 gcggcgaaaa agagtctggg actcgaagaa aagcagaggt atatccttgt ggctggcggc
     1381 agcatgggag cgggatcgat cgaccggctg attccggcat tgctgcggcg catgcaaagg
     1441 gaggagcatc tcatcctgat ctgcggcagc aatcagaagc tagagcagcg gatgcgtgcg
     1501 cgcttcggca gagatgcgag ggtgacgatc ctgggaagcg tcagcaacat gccggtttat
     1561 ctgcatgcgt gtgacatcat ttacacaaaa cccggcggac ttacctccac ggaggccgca
     1621 gtgacggaga ttcccattgt ccactcaaag ccgatacccg gatgtgagac aaagaaccgg
     1681 agcttttttg tcaaaaaggg aatgagcgtg accgcataca cggaatatgg cctggtcaga
     1741 aaaggaatgc agttgcttca taattccggt aagaaaaacg caatgcagag agcacagtgc
     1801 cagggaatcg aaaaacacag tgcggaaaag atttatcagt ttgtaaggga aaagggcatt
     1861 tattaaattt ttttaaatgg atccgtatac tct
//
LOCUS       DBMDQJ010000502         1891 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20681_length_1891_cov_1.582123, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000502 DBMDQJ010000000
VERSION     DBMDQJ010000502.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20681_length_1891_cov_1.582123"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="ACZO9R_09650"
     CDS             <1..228
                     /locus_tag="ACZO9R_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HIR0913361.1"
                     /translation="GAKIIEAVIRMAESLDMMVIAEGVEEKHQVDFLNDLGCDYIQGY
                     YFGRPMSQDQYEKLTNHDEEEQHDMPQPESS"
     gene            289..813
                     /locus_tag="ACZO9R_09655"
     CDS             289..813
                     /locus_tag="ACZO9R_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HIR0913362.1"
                     /translation="MGVMIFLSDIMIPLLIFGIVGYGILNRQNIYEEFIDGAKDGFQT
                     VIGIMPTLIGLMVAVGILRASGFLECFSGLLGRGIGWLGFPPELIPVTVVKMFSSSAA
                     TGLLLDIYKEYGADSMLGKMASIMMSSTETIFYTMSVYFMAAGIKKSRYTLVGALIAT
                     TAGTVASIILAGYM"
     gene            1051..1124
                     /locus_tag="ACZO9R_09660"
     tRNA            1051..1124
                     /locus_tag="ACZO9R_09660"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1085..1087,aa:Met,seq:cat)
     gene            1309..>1891
                     /locus_tag="ACZO9R_09665"
     CDS             1309..>1891
                     /locus_tag="ACZO9R_09665"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034979 - NAD-dependent protein lysine
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HIR0913363.1"
                     /translation="MTNIEQFLQMVKESDNIVFFGGAGVSTESGIPDFRSVDGLYNQK
                     YDYPPETILSHSFYIKYPEEFYRFYRDKMLCLDAEPNITHKKLAELEAAGKVKAVVTQ
                     NIDGLHQLAGSKRVLELHGSVHRNYCRKCGKGFDAEYVRDYPGKVPLCDACGGTIKPD
                     VVLYEEGLDQQTLEDAVFYISHADMLIIGGTSLA"
BASE COUNT          481 a          364 c          566 g          480 t
ORIGIN      
        1 ggtgcaaaga tcatcgaggc ggtcatccgg atggcggaat ctctggacat gatggtgatt
       61 gcggaaggtg tggaggaaaa gcatcaggtg gattttttaa atgacctcgg atgcgactat
      121 attcagggtt attattttgg ccgccccatg tcgcaggatc agtatgagaa gctgacgaat
      181 cacgatgagg aagagcagca tgacatgccg cagcccgaaa gttcgtgaat atgaatattt
      241 accgttcgga ttccatatag tagaataacg gaagattctg gatgtatcat gggtgtaatg
      301 atttttctat cggacataat gataccgctt ctcattttcg gtattgtcgg ttatggaatt
      361 ctgaacagac agaatatcta tgaagaattc atagacgggg caaaggacgg ctttcagacg
      421 gtcatcggaa tcatgcccac actgatcggg cttatggtgg cggtcgggat tctgcgggct
      481 tccggttttc tggagtgttt ttccgggctg ctgggacggg gaatcggctg gctgggtttt
      541 ccgccggagc tgataccggt cacggtggtc aaaatgttct cgtcctctgc ggcgaccggt
      601 cttcttttgg atatatataa ggagtacggc gcggattcga tgctggggaa gatggcatcc
      661 atcatgatga gcagcacgga gacgatcttt tatacgatgt cggtctattt tatggcggcg
      721 gggatcaaaa aaagccgtta cacgctggtg ggcgcgctga tcgcaaccac tgcgggaacc
      781 gtcgcaagca ttatcctggc gggatatatg tgagcagccg gcggctgtgg ctgcgcccgg
      841 gaaggatttt ttctgcaaaa atgtctgtga aaacagaaaa atgaaaaaat cggttctata
      901 ggatgtcagc tctgcatata atgatagagt aaggcaggaa aaagcagggt gcggaagcgg
      961 atttgacaag tgggtacgaa agagttataa tacattatgt taacaagcaa cggacattgt
     1021 gatacaggaa gcaatggtgg ttgcatatag cgcgggatgg agcagtctgg aagctcgtcg
     1081 ggctcataac ccgaaggtca taggttcaaa tcctattccc gcaacttttt aggcagcagt
     1141 cgcatgactg ctgttttttt gctttatcta aaagacctgg gttctgctgt agctgatgtt
     1201 tgcgttttag agaacgtttt ccggatctgc ggacggaata ccaatagatt ttggcgtgcg
     1261 gttggtgtat gatagagaaa atcgcagtgg gatgggaggc gccagtcaat gacgaatatc
     1321 gaacaatttt tgcagatggt gaaggagtcg gacaatattg tattcttcgg gggagccggt
     1381 gtatctacgg agagcggaat tcctgatttc cgcagtgtgg atggtcttta caaccagaaa
     1441 tatgactatc cgccggagac gattttaagc cattcctttt atataaaata cccggaggag
     1501 ttttaccgtt tttaccggga caaaatgctc tgccttgacg cggaaccgaa catcacgcat
     1561 aaaaagctgg cggagcttga ggcggccgga aaagtaaaag ccgtcgtgac gcagaatatt
     1621 gacggtctgc atcagctggc gggaagtaag cgcgtgcttg aactgcacgg cagcgtgcac
     1681 cgtaattact gcaggaagtg cggcaaggga tttgatgcgg agtatgtgag ggattatccg
     1741 ggaaaagtac ctctgtgcga tgcctgcggc ggtacgatca agccggatgt ggtactctat
     1801 gaggaaggac tggaccagca gacgctggag gatgcggtat tttatatcag ccatgcggat
     1861 atgctgatta tcggtggaac atcgcttgcg g
//
LOCUS       DBMDQJ010000503         1889 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20712_length_1889_cov_1.540268, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000503 DBMDQJ010000000
VERSION     DBMDQJ010000503.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20712_length_1889_cov_1.540268"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            24..206
                     /locus_tag="ACZO9R_09670"
     CDS             24..206
                     /locus_tag="ACZO9R_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HIR0913364.1"
                     /translation="MAVPQAKEAMNRFKQEVASEIGVPLKDGYNGDLTSAQAGSIGGE
                     MVKKMIMKQEEQMSGK"
     gene            complement(894..>1889)
                     /locus_tag="ACZO9R_09675"
     CDS             complement(894..>1889)
                     /locus_tag="ACZO9R_09675"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HIR0913365.1"
                     /translation="TVEGEAFTKEYELPNDMNYAETCASIGLVFFARNMLKTEKNGRY
                     ADVMERALYNGIISGMQLDGKRFFYVNPLEVNPGVSGEIFGYKHVIPERPGWYACACC
                     PPNLVRMVTSLGKYAWDEDETAVYSHLFLGQEAALGKADIRVESAYPWEGSVTYHVSA
                     KIDELFTLAIHIPAYMKDLRVTVNGEAFDTAGEIRDGYLYISRKWGSDDQVELHFPLP
                     VRKIYASTHVREDVGCVALMRGPVVYCFEGADNGANLQALAVKKELDAKALVCTEGRL
                     SGLTLLDVAGIRLVPSEELYTEEPPKEETVTLRAIPYFAWGNRGLNQMRVWMHEK"
BASE COUNT          460 a          525 c          454 g          450 t
ORIGIN      
        1 attctaattc cggcacaaac cagatggcag tgccgcaggc aaaagaggca atgaaccgtt
       61 ttaagcagga agttgcaagt gagatcggtg taccgttaaa ggacggctac aacggcgact
      121 tgacttccgc acaggccgga agcatcggcg gcgagatggt caagaaaatg atcatgaagc
      181 aggaagagca gatgtccggc aaataaaccg gctgcttctg ccgattgcac aaaaacgggg
      241 ctgtcgcttt aagcaggcag tcccgtttgg catgagaaga aataggacga gagaatcatg
      301 cccttcgatt tcagatagaa tgcagatgga atattttttg tgaaaaaatg agagcatttc
      361 tttctgccgg atatttgcat cggattccgt gtctcttcca cgtttggaca atccgacggg
      421 gacatgtccg gcacaccaaa gacaatgagc ggactttcta gccgccctaa ctgtactccc
      481 tcatagacaa tataaaaccg cgcgaaccga aactcgttgc catctgctat taaaaacaag
      541 gcagatgaca attcagacat cggcttcgcg cggttatgtt ttattgcgac ataaggatgg
      601 ctgatttatg ccatccgttg tttggtactg gtaagggcgg cacaaaaggc ctacaaattg
      661 tcttcggggt gcggacatga cccgtggagt gttccaaact tggaaagaca cggatcatcg
      721 cggatgcaaa tatccggctg gctagaaatg ccgcattttt gaacagaatt tccatctgca
      781 tttccatctg aaatcgcgag gcatgatctt cttgtcctat tacttatcat tccattacgc
      841 atcacaccaa atgggactgc ccgtttcatg cggcagcccc attttttgct tttttatttc
      901 tcgtgcatcc agacgcgcat ctggtttaaa ccacggttgc cccatgcaaa ataagggatt
      961 gcgcgaagcg taaccgtttc ttccttcggc ggttcctcgg tgtagagttc ctccgacggc
     1021 acaagccgga tccccgccac atcgagcagt gtgagaccgg aaagcctgcc ttccgtgcat
     1081 acgagagcct ttgcatccag ttcttttttt accgcaagcg cctgcagatt ggctccgttg
     1141 tcagcgccct caaagcagta gaccacaggt ccgcgcatca gcgccacgca tccgacgtcc
     1201 tcgcgcacat gcgtactcgc ataaattttg cgcaccggca gtggaaaatg aagttccacc
     1261 tgatcgtcgc ttccccattt tctgctgata tacaaatagc catcacggat ttctccggca
     1321 gtatcaaacg cctccccgtt gaccgtcacg cgcaggtctt tcatataagc cgggatatga
     1381 atggcaagtg taaagagttc gtcgatcttc gcggacacat ggtaggtcac gcttccctcc
     1441 cacgggtatg cgctctccac gcggatgtcc gccttgccga gcgccgcctc ctgtcccaga
     1501 aacagatggg agtacaccgc cgtctcgtcc tcatcccacg catattttcc aagggaggtc
     1561 accatgcgga ccaggttcgg cgggcagcat gcgcacgcat accagcccgg acgctccgga
     1621 atcacatgtt tgtaaccgaa aatctcgccg gacacacccg gattgacctc cagcggattg
     1681 acgtagaaaa accgctttcc gtcgagctgc ataccgctga tgatgccatt gtagagcgcc
     1741 cgctccatca catcggcata tcttccgttc ttctccgtct ttaacatatt tctcgcaaaa
     1801 aagaccagac cgatcgacgc acaggtctcc gcatagttca tatcgttcgg cagttcatac
     1861 tcttttgtga atgcctcgcc ctccacggt
//
LOCUS       DBMDQJ010000504         1885 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20765_length_1885_cov_1.483184, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000504 DBMDQJ010000000
VERSION     DBMDQJ010000504.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20765_length_1885_cov_1.483184"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="ACZO9R_09680"
     CDS             complement(<1..311)
                     /locus_tag="ACZO9R_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0913366.1"
                     /translation="MATLKDVAKETGLTVTTVSRVLNNRGYISEETRRKVYDAMKKLN
                     YHPNEVARSLSKQSTNTIGVIVPHIRDPYFAELISNLESQAYRHQHKILLFNSQEKNE
                     K"
     gene            complement(317..1867)
                     /locus_tag="ACZO9R_09685"
     CDS             complement(317..1867)
                     /locus_tag="ACZO9R_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HIR0913367.1"
                     /translation="MEKQIKIKADTLFLPICAGKKTELMECFCEGKKVMELQVPVDIQ
                     EKNGYHIDYYARFPVKQFIGKTLILRGHMPESFLEEIRNDAVCDTAASDRTDSDRPRR
                     PRIHFTVQTGWINDPNGLVYKDGYFHLYFQYNPCDIAWENMCWGHAVSRDLLHWTQQD
                     TVLYPDEHGTVFSGSGVVNEHGMLGLPESALLFFYTAAGACNRWCENDNSTQRLAYSL
                     DGGATLQKMNWELATICKENRDPKVFWHEESRAYVMVLWLEKNDFGILRSDDLRNWRM
                     TDRLTLDQAWECPDLIRLPGADGGAEWMFWSADGYYFWGTFDGFRFQTDGIRHEAYLG
                     KLPYAAQTYAGVSDRVISVPWLRLPNRGTLYTGAMGLPRELSVVRLDGEKYLAQSLPL
                     EWRKNCVPVDLTEMERGERLTYQYHGGEEVIELRVCLREDAQEMALRLGKDALTYDKK
                     SGILSVNGTDYPIGCDVRDFSFLLDDVLLEVTAKYDTIIGVFELQERECIIEIERDAF
                     EKMQMNRM"
BASE COUNT          429 a          567 c          385 g          504 t
ORIGIN      
        1 tccttctcat tcttctcctg cgaattaaag agcagaattt tatgctgatg gcggtaggcc
       61 tgactttcca gattgctgat cagctccgca aaatagggat ctctgatatg cggaacaatc
      121 acgccgatgg tattcgtgga ctgtttggaa agtgagcgcg ccacctcatt cgggtgataa
      181 ttcagttttt tcattgcatc gtacaccttc ctgcgcgtct cctcgctgat gtatccgcgg
      241 ttatttaaca cgcgggacac cgtcgtgacc gtaagtccgg tttcctttgc cacatccttc
      301 aatgttgcca taactttcac atcctgttca tctgcatttt ctcaaatgca tctcgctcta
      361 tctcaatgat acattcccgt tcctgcaatt caaatactcc tattattgta tcatactttg
      421 cagtcacttc aaggagcaca tcatccagca aaaacgaaaa atctctgaca tcacagccaa
      481 tcggataatc cgtcccgttc acggaaagga ttccgctttt tttatcgtag gtaagcgcat
      541 cttttccgag gcgtaaagcc atctcctgtg catcctcccg caggcacacc cgcagttcaa
      601 tgacttcctc cccgccatga tactgatacg tcagacgctc tcccctctcc atttctgtaa
      661 gatctaccgg gacacagttc tttctccact cgagcggcag actctgcgcc agatattttt
      721 caccatccag ccgaaccacc gaaagttccc gcggcagtcc catcgctcct gtatacaagg
      781 taccacggtt tggcaggcgc agccacggca ccgaaatcac gcggtcactc accccggcat
      841 aggtctgcgc cgcatacggc agcttcccga gatatgcctc atgccggata ccatccgtct
      901 gaaaccggaa tccgtcaaaa gtcccccaga aatagtagcc atccgcgctc cagaacatcc
      961 actccgcacc gccgtccgca ccgggcagcc ggatcagatc cgggcactcc catgcctgat
     1021 ccagtgtgag acgatccgtc attctccagt ttctcagatc gtctgatctc aggatgccaa
     1081 aatcattttt ctccagccac agtaccatga cgtaagcccg gctctcctcg tgccagaata
     1141 ctttcgggtc acggttctcc ttgcatattg tagcaagctc ccagttcatt ttctgaagcg
     1201 tcgccccgcc atcaaggctg tacgccagac gctgggtgga attatcattt tcacaccacc
     1261 ggttgcatgc gcctgccgcc gtatagaaaa acagtagtgc gctctccggc agtccgagca
     1321 ttccatgttc atttaccacg ccgctcccgg aaaacacggt tccgtgttcg tccggataca
     1381 gcacagtatc ctgctgggtc cagtgcagca gatccctgct caccgcatgt ccccagcaca
     1441 tattttccca tgcaatatca cagggattgt attgaaaata cagatgaaag tatccatcct
     1501 tatatactaa tccattggga tcgttgatcc atccggtctg caccgtaaaa tggattcggg
     1561 gacgccgggg tctgtcgcta tctgtccgat cagacgcggc agtatcacac actgcgtcat
     1621 ttctgatctc ctccagaaaa gactccggca tatgtcccct taggattagg gttttaccga
     1681 taaattgctt taccggaaaa cgggcataat aatcaatgtg gtatccgttt ttctcctgga
     1741 tatccaccgg aacctgaagt tccatgactt ttttcccttc gcagaaacac tccatgagtt
     1801 ctgtcttctt gcctgcgcag atcggcagga aaagtgtatc cgcttttatt tttatctgtt
     1861 tttccatgat accatcctcc tctat
//
LOCUS       DBMDQJ010000505         1882 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20806_length_1882_cov_1.091522, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000505 DBMDQJ010000000
VERSION     DBMDQJ010000505.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20806_length_1882_cov_1.091522"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="ACZO9R_09690"
     CDS             complement(<1..356)
                     /locus_tag="ACZO9R_09690"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0913368.1"
                     /translation="MLELIKESDLDTVRHFYRALAGTEFCAWTDIYPGEPELQSDFSR
                     DSLFCIRDDVSGEIAGLVSIDDDPEVESIACWSEELQPSAEISRLGVAEKYQNRGIAR
                     ELLAGAMEELRRRGCK"
     gene            complement(590..>1882)
                     /gene="dnaB"
                     /locus_tag="ACZO9R_09695"
     CDS             complement(590..>1882)
                     /gene="dnaB"
                     /locus_tag="ACZO9R_09695"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR0913369.1"
                     /translation="VEAEQSVIGSMLMDREAIIAASEIITGADFYQHQYGVMFESMVE
                     LFNEGKPVDLVTLQNRLREKDVPPEVSSLDFVRDIMTSVPTSVNVKSYANIVREKAVL
                     RRLIKVNEEIENLCYGGRDKLEDILAHTEKSVFDLLQSRGGGEFVPIRQVALNVLEKI
                     EAASKSNSTVTGIPTGFIDLDYRTSGFQPSDFILIAARPSMGKTAFVLNVVEHVAVKK
                     GLPCMVFSLEMSKEQLVNRMLSMESNVDSQKLRTGTLTDSDWDAVVEGIGTIGSSKLI
                     IDDTPGISISELRSKCRKVKLEYGLSLVIIDYLQLMSGSGKSGENRQQEISEISRSLK
                     ALARELNAPVISLSQLSRACETRPDHRPMLSDLRESGAIEQDADVVMFLYRDDYYNKD
                     TDTPNIAEVIIAKQRNGPIGTVQLMWRPELTKFANLAK"
BASE COUNT          454 a          578 c          415 g          435 t
ORIGIN      
        1 ctcttgcagc cgcgtcttct aagttcctcc atcgcgcctg ccagaagctc gcgtgcgatg
       61 cctcggttct ggtacttctc cgccacaccc agtctggaaa tttctgccga cggctgcagc
      121 tcctccgacc agcaagcaat cgattccacc tccggatcat catcgatcga aaccagccct
      181 gcgatctccc ccgacacatc atcccggata caaaaaagcg aatccctaga aaaatccgac
      241 tgtagctccg gctcccctgg atagatgtcc gtccacgcgc agaactcggt tccagctaag
      301 gcacggtaaa aatggcggac ggtgtccaga tccgattctt ttatcaattc aagcatttct
      361 catattcctt tttacataat aatatttatc caaaaaaaag ggactggatg aaaattgcag
      421 agtcggcgtt ccaattatga attgtacgaa tcttgaacac acgcgtccga acagggcgaa
      481 tgcagtccgc catgggcgaa cacgtgtagt gaaagcaagc gcacaatcat caccggagac
      541 gcagacggtg caatttccat ccagtcccat cccttacacg attctatcgt tatttcgcca
      601 aattcgcaaa cttcgtcagc tccggtctcc acatcagctg caccgttcca atcggaccat
      661 tacgctgttt ggcaatgatt acctccgcga tattcggcgt atccgtatcc ttgttgtaat
      721 aatcatcacg gtacaaaaac ataaccacat cggcatcctg ctcaatcgca ccagactcac
      781 gcagatccga aagcatcggg cggtggtccg gacgtgtctc gcaggcacgg gaaagctgcg
      841 acagggagat aaccggcgca ttcagctctc gcgccagcgc ctttagtgaa cgggaaatct
      901 ccgagatctc ctgctggcgg ttctctccgc tctttccgct gccgctcatc agctgtaaat
      961 agtcgatgat gacaaggctt aaaccgtact ccagcttcac cttgcggcat ttcgagcgca
     1021 gctccgagat cgagataccc ggcgtatcat cgataatcag cttggaactt ccgatcgtgc
     1081 cgatgccttc cacgacggca tcccagtcgg aatccgtcag cgtaccggta cgcagcttct
     1141 gggaatcgac attcgactcc atcgacagca tacggttgac cagctgttcc ttcgacattt
     1201 ccaggctgaa taccatgcac ggaagccctt ttttgaccgc cacatgctcg acgacattca
     1261 gaacaaacgc cgtcttaccc atcgaaggac gcgccgcaat caaaataaaa tcggacggct
     1321 ggaatcccga cgtccggtaa tccagatcga taaatccggt cggaataccc gtgacggtgc
     1381 tgttgctctt cgacgccgcc tcaatctttt ccagaacatt caatgccacc tgccggatcg
     1441 gcacaaactc tccgcctccg cggctctgga gcaggtcaaa caccgatttc tccgtgtgcg
     1501 ccagaatatc ctccagcttg tcccttccgc cgtagcagag attttcaatc tcctcattca
     1561 cttttatcaa acggcgcagg actgcctttt cccgcacgat attggcatac gatttgacat
     1621 tgaccgaggt cggcacagat gtcatgatat cccggacaaa gtccaggctg ctgacctccg
     1681 gcggaacatc tttttcgcgc agccgatttt gaagcgtcac cagatcgacc ggctttcctt
     1741 cgttgaaaag ctcgaccatc gactcaaaca taacgccata ctggtgctgg taaaaatcgg
     1801 cgcctgtgat aatctcagac gccgcgataa ttgcttcccg atccataagc atggatccga
     1861 tgactgactg ttccgcctct ac
//
LOCUS       DBMDQJ010000506         1881 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20816_length_1881_cov_1.402247, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000506 DBMDQJ010000000
VERSION     DBMDQJ010000506.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20816_length_1881_cov_1.402247"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="ACZO9R_09700"
     CDS             <1..200
                     /locus_tag="ACZO9R_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913370.1"
                     /translation="GRWGIPTIRRTMQEYHLPQPEFLDERRSFIAMKGKRIKDKDSVN
                     RTRIALSKTSLQCIIALMICE"
     gene            219..917
                     /gene="pyrF"
                     /locus_tag="ACZO9R_09705"
     CDS             219..917
                     /gene="pyrF"
                     /locus_tag="ACZO9R_09705"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR0913371.1"
                     /translation="MGKDVIIACDFNSKAEVIAFLEKFKDEKPFVKIGMELFYAEGPD
                     IVRTIKGMGHKIFLDLKLHDIPNTVKKAMAVLSGLDVDMCNVHAAGTKGMMQAALEGL
                     TRADGSRPLLIAVTQLTSTSEEVMQQELWIDKPIDETVMHYAKNTMEAGLDGVVCSPL
                     EAGKVHAVCGEKFLTITPGVRFADGDVGDQKRVTTPAKAKELGSDYIVVGRPITQAAD
                     PVAAYRRCVEEFVG"
     gene            998..1723
                     /locus_tag="ACZO9R_09710"
     CDS             998..1723
                     /locus_tag="ACZO9R_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HIR0913372.1"
                     /translation="MPQKGFSGSTLKLIAIITMLIDHIGAAVIARLLIAGQGSEMLYK
                     IYYAMRAVGRVAFPIFCFLLVEGFFYTGSRKKYALRLFGFALLSEIPFDLAFSGKILE
                     FGYQNVFFTLLIGLLTIMLFDAVVKKQEWHPVLRSALLVIITFGGMGAAYLLHTDYDA
                     KGVLAILVFYMTRQMRGLQIVAGCLAFCWELPAIVAFIPIAFYNGKRGWNIKYLFYAF
                     YPVHLLVLYLICVGMRTAQIAVV"
BASE COUNT          481 a          362 c          589 g          449 t
ORIGIN      
        1 caggacgatg gggaatcccg acaatacgca gaacgatgca ggaatatcac ttgccacaac
       61 cggaattttt ggatgaaaga aggagtttta ttgcgatgaa aggaaaaagg atcaaggata
      121 aagattctgt caacagaacg cgaattgcct tgtcaaagac ttcgctgcag tgtataattg
      181 cactgatgat ttgcgagtga agaacacggg aggaagacat gggaaaagac gttattattg
      241 catgtgattt taacagtaag gcagaagtca ttgcgtttct tgagaagttc aaggacgaga
      301 agccgttcgt caagatcgga atggagcttt tttatgcgga aggaccggac atcgtgcgta
      361 ccatcaaggg gatgggacac aagatcttcc ttgatctgaa gcttcatgac attccgaaca
      421 cggtgaaaaa ggcgatggcg gttctctcgg gactggatgt cgatatgtgc aatgtacatg
      481 cagcgggaac gaagggcatg atgcaggcgg cactggaggg actgacgaga gcggacggca
      541 gcagaccgct tctgatcgcg gtgacgcagc ttacctccac gagcgaggaa gtgatgcagc
      601 aggagctgtg gattgacaag ccgatcgatg agacggtcat gcactatgcg aaaaatacca
      661 tggaagcagg actggatgga gtcgtatgct cgccgcttga ggcaggcaag gtacatgcag
      721 tctgcggaga aaagtttttg acgatcacgc cgggcgtgcg tttcgcggac ggcgatgtgg
      781 gagatcagaa gcgtgtgacg acacctgcaa aggcaaagga actcggatcg gattacatcg
      841 tagtcggaag accgatcaca caggcggcgg atccggtagc ggcataccgc agatgtgtgg
      901 aagaatttgt aggttaggac gacatacacg aagaggagat agacgaaaca gcctggtctc
      961 ctcttttttt cataggaaga aaaggacggt ggggaagatg cctcaaaaag gattcagcgg
     1021 aagcacattg aagttgatcg cgatcatcac gatgctgatc gaccatatcg gagcggcggt
     1081 cattgccagg ctgctgattg ccgggcaggg gagtgagatg ctctataaga tctattatgc
     1141 gatgcgcgcc gtcggcagag tggcattccc tattttctgt tttctgcttg tggagggatt
     1201 tttttacacg ggcagccgga aaaaatatgc acttcggctg tttggatttg ccctgctgtc
     1261 ggagataccg ttcgatctgg cattttccgg gaaaatactg gaattcggtt atcagaatgt
     1321 atttttcacg ctgctgatcg ggctgcttac gatcatgctg tttgatgcgg tggtgaaaaa
     1381 gcaggagtgg catccggttt tgcgcagtgc gctgcttgtc atcatcacat tcgggggaat
     1441 gggtgcggcc tatctgctgc acacagatta tgatgcaaaa ggcgtgctcg caattctggt
     1501 gttttatatg acgagacaga tgcggggatt acagatcgtg gcaggctgcc tggcgttctg
     1561 ctgggaactt ccggcgatcg tggcgtttat cccgattgcg ttctataacg ggaagcgcgg
     1621 atggaacata aagtacctgt tctatgcgtt ctatccggtg catctattgg ttctttatct
     1681 gatctgtgta gggatgagaa ccgcgcagat tgcggtggtg taggagagaa agggaggaag
     1741 agagtaagta tggagatatc aaaggagacg cggaaacgga tgatgcagat cattgcattt
     1801 gggattctgc tctattgcgg gatggagcat tttgacgtgg tgatcggagc ggtggatttt
     1861 gtgctgggga ttgtgatgcc g
//
LOCUS       DBMDQJ010000507         1878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20857_length_1878_cov_1.434440, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000507 DBMDQJ010000000
VERSION     DBMDQJ010000507.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20857_length_1878_cov_1.434440"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="ACZO9R_09715"
     CDS             <1..329
                     /locus_tag="ACZO9R_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HIR0913373.1"
                     /translation="YSAMIPAHEDCSHLQIGDVIEAKVTGVKPDGKLDITMREKAYLQ
                     MDADAEKVMEVIEEFAGVLPFTDKASPEVIMRETGLSKNAFKRAVGRLYKERRIEITE
                     RAIRKL"
     gene            398..772
                     /locus_tag="ACZO9R_09720"
     CDS             398..772
                     /locus_tag="ACZO9R_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR0913374.1"
                     /translation="MKNVISTDKAPAAIGPYSQAIEVNGMVYTSGIIPVVPATGEIPE
                     GSVAQAEQALTNLSHLLETAGTSMANVVKTTVFIKEMNDFAAINEVYATFFEGAYPAR
                     SCVEVARLPKDVMLEIEAIATK"
     gene            complement(897..1109)
                     /locus_tag="ACZO9R_09725"
     CDS             complement(897..1109)
                     /locus_tag="ACZO9R_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfB family protein"
                     /protein_id="HIR0913375.1"
                     /translation="MDFSNVDLALGTLTAYDSGSSVSSGSLAYAVGTAVLSQSLDLTQ
                     QMGASMIQMMEHSVNPELGGNIDMYV"
     gene            1498..>1878
                     /locus_tag="ACZO9R_09730"
     CDS             1498..>1878
                     /locus_tag="ACZO9R_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR0913376.1"
                     /translation="MIYAAPVAGVSVATTAEAVAVGTGSSSPSAGVSLAVADCLVASN
                     EEVAQEAAAQQEQVSENETPVVESEYADIAIAQVDNYVNVRTEPNTESEVVGKLYNNS
                     AATILQTTEDGWYEITSGNVTGYVK"
BASE COUNT          551 a          396 c          539 g          392 t
ORIGIN      
        1 gctactcggc gatgataccg gcacatgagg actgcagcca tcttcagatc ggcgatgtga
       61 ttgaggcgaa ggtcaccggg gtgaagccgg acggcaagct tgacatcacg atgcgggaaa
      121 aggcatatct gcagatggat gcggacgccg agaaggtcat ggaagtgatt gaggaattcg
      181 cgggcgtgct tccgtttacg gacaaggctt caccggaagt catcatgcgt gagacggggc
      241 tgagcaaaaa tgcattcaag cgtgcggtcg gcagactgta caaggagcgc cgcatcgaga
      301 ttacggagcg cgcaatccgc aaattatagt agcatcgctg tttcaggtgt gctatgatag
      361 atgaaatcga cagactataa gaaagaagga atacagcatg aaaaatgtaa tcagcacgga
      421 caaggcaccg gcagcgatcg gaccatattc ccaggcgatt gaggtaaacg gcatggtata
      481 cacatccggt attattccgg ttgtaccggc aacaggcgag atcccggagg gatctgtggc
      541 gcaggcagag caggcgctga cgaatctgtc ccatctgctt gagacagcag gcaccagcat
      601 ggcgaacgtg gtaaaaacaa ccgtatttat taaggaaatg aatgatttcg cagcgatcaa
      661 cgaagtatat gcgacatttt tcgagggcgc gtacccggcg agaagctgcg ttgaggtggc
      721 acgtcttccg aaggacgtta tgctcgagat cgaagcgatt gctaccaaat aattgctgcg
      781 acaggaacag gaatacctga gactgccgga ggagatctgg aacataaaaa gacgcggcac
      841 actgttacat cgtgtgccgc gtcttttctt ttcagagagc atctgttaca tgatccctat
      901 acgtacatat cgatattgcc gccgagctcc gggttcaccg aatgttccat catctgaatc
      961 atggaggcgc ccatctgctg ggtcaggtct aatgactggc tgagaacggc cgtgccgact
     1021 gcataggcga gactgccgga gctgacagaa gaaccggagt cgtaggccgt cagtgtgcca
     1081 agcgccagat caacattgga aaaatccata cgaatcccac ctttccttct tatgatagct
     1141 ttattatgcc acagatggag gagaatggca attatgaaaa atgcaacaaa ttataaaacc
     1201 ggaaaggcat aaggcagcag caggaaggga aaatctggga gcatatgtaa cccggatttg
     1261 tcggaaaatg gcgcagattg tggaaaagac gtgtcgaaaa agaaaatatt gtgtaaaatc
     1321 cgcggagtat tacaaaatat agcgggttga catcatatag acgctttgtt ataataacca
     1381 tgtaataaat ataacaaatt tgtaataaat ccgttggagg cattcatgaa cagaagaaca
     1441 ctgagagcta cagggattgt attgactgga atgttattgg caggagatcc tgtatctatg
     1501 atatatgcag caccggttgc gggtgtgtcc gttgctacca cagcagaagc agtggcagtc
     1561 ggaacgggaa gcagcagccc gagcgcaggc gtttcgcttg cagtggcaga ttgtctggtc
     1621 gcaagcaatg aggaagtggc gcaggaagca gcagcgcagc aggaacaggt aagcgagaat
     1681 gagacaccgg tggtagagtc cgaatatgca gacattgcca ttgcgcaggt agataactac
     1741 gtaaacgtgc gtaccgagcc gaacacagag agcgaggtag tcggtaagct ttacaataat
     1801 tcagcggcaa cgattttaca gaccacagag gatggctggt atgagatcac atctggcaat
     1861 gttaccggtt atgtgaag
//
LOCUS       DBMDQJ010000508         1878 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20862_length_1878_cov_1.238604, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000508 DBMDQJ010000000
VERSION     DBMDQJ010000508.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20862_length_1878_cov_1.238604"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(67..1437)
                     /locus_tag="ACZO9R_09735"
     CDS             complement(67..1437)
                     /locus_tag="ACZO9R_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HIR0913377.1"
                     /translation="MNYSMVLYILGYILKFESAFLMLPALVSLIYQERAGIPFVITAA
                     ICLVLGIVFTHKKPSSSTVYTREGFVTVALSWIVMSIFGAIPFVWNGDIPYYVDALFE
                     TVSGFTTTGSSILSDVESLSHASLFWRSFSHWIGGMGVFVFIMSILPLMGGSTINLMK
                     AESPGPSVSRLVPHIKDTAKILYGIYLAITLLEIVILCALGMPLFESLLTSFGTTGTG
                     GFGFRNDSFASFSPAIQNTVTTFMILSGINYTFYFCILSRRIKDAFRIEEVRWYLFLI
                     FASVGMITYNIRPLYANTRDALRNAFFQVGAIITTTGYSTADFDMWPALSQTILVTLM
                     FVGACAGSTGGGMKVSRLVILFKTIRKELSLIIHPREIRKIRMDGHVVEHSTLRSTNV
                     FLVIYFILLMLSTLIISLDEFDFTTNFTAVVSCLNNIGPGLNRVGPAQNFSIFSPPTF
                     RIHHVL"
     gene            complement(1444..>1878)
                     /locus_tag="ACZO9R_09740"
     CDS             complement(1444..>1878)
                     /locus_tag="ACZO9R_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015519992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HIR0913378.1"
                     /translation="ILLSLYANDVSNAKTVTKINRSSFNSVINEMNLGSIIYPRVITA
                     DYILKYVRSTVNSINSNVETLYKLAGGKAEALEFIIKENSAVAGIPLQDLNFRKNTLI
                     CCIYRNGKVILPGGQDMMLAGDSVLVVISGYRISDIKEILED"
BASE COUNT          555 a          432 c          486 g          405 t
ORIGIN      
        1 aaacaagaat catggagata tacgaaaacc ttaaagccgg aaaagagctt gacggacgag
       61 gcaatattaa agaacgtgat gaatacgaaa ggtaggcggc gagaagatcg aaaaattctg
      121 cgccggtccg acacggttta agccaggacc gatgttgttc agacacgaga cgactgctgt
      181 aaagttcgtg gtaaaatcga actcgtccag gctgatgata agcgttgaca gcatgagcaa
      241 aataaagtaa atgaccagaa aaacattggt agagcgcagg gtgctgtgtt ctacgacatg
      301 tccgtccatg cggatcttgc ggatctcgcg ggggtggata atcagggaaa gctctttgcg
      361 gatggttttg aataaaatca ccagccggga tactttcata ccgccgcctg tgcttcccgc
      421 acatgcgccg acgaacatca gcgtcacaag aatcgtctgg gagagcgcag gccacatgtc
      481 gaagtcggca gtggaatatc cggtggtggt gatgatcgca ccgacctgga aaaaggcatt
      541 ccgcagcgcg tccctggtat ttgcgtaaag cgggcgtata ttataggtaa tcattccgac
      601 agaagcaaag atcaaaaaga gataccagcg cacttcctcg atgcggaatg catccttgat
      661 tctgcgcgat aaaatacaaa aataaaatgt gtaattgata ccgcttaaga tcataaaagt
      721 tgtgaccgtg ttctggatag caggcgaaaa gcttgcaaag ctgtcgttac ggaaaccgaa
      781 accaccggtg ccggtcgtgc cgaaactcgt cagaagagac tcaaacagcg gcatgccgag
      841 cgcacacaga atgacaatct caaggagcgt gatggcaagg taaatgccgt ataatatttt
      901 cgcggtatcc ttgatgtgcg gaaccagacg gctgacggaa ggtcccgggc tctccgcctt
      961 catgaggttg atcgtagagc cgcccatcag aggcaggatc gacataataa agacgaacac
     1021 acccatgccg ccgatccagt ggctgaaact gcgccagaaa aggctggcat gcgacaggga
     1081 ctcgacatcg gagagaatac ttgaaccggt cgtggtaaaa ccggagaccg tttcaaacag
     1141 ggcgtctaca taatatggaa tgtcgccgtt ccacacgaag gggatagcgc cgaaaatact
     1201 cataacgatc cagctcaagg cgacagtaac aaacccctcc cttgtataca cggtcgagga
     1261 agacggtttt ttatgtgtga aaacaatccc taaaaccaga cagattgcag cggtaatgac
     1321 aaacggaatg cctgcccttt cctgatagat caacgatacc agtgccggga gcatcagaaa
     1381 cgcgctctca aattttaaaa tatagcccag aatatataaa accatggaat aattcattgc
     1441 aggttaatcc tccagaatct ctttgatgtc ggagatgcgg tatccggaaa tgacgaccag
     1501 aacggagtct ccggcgagca tcatatcctg tccacccggc agaatgacct taccgttgcg
     1561 gtagatgcag cagattaaag tattcttgcg gaaattcaga tcctgcagcg gaatgcctgc
     1621 gacggcagaa ttctccttga tgatgaattc cagagcctcg gcttttccgc cggcaagctt
     1681 gtaaagtgtc tccacgttgc tgttgatgga attcactgtg gagcgcacat attttaaaat
     1741 gtaatcagcg gtaatgacac gcggataaat aatacttccc agattcatct cgttgatgac
     1801 ggagttaaaa ctgctccggt tgatcttggt caccgttttt gcattggaga catcgttggc
     1861 gtacagcgac agcaggat
//
LOCUS       DBMDQJ010000509         1877 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_20883_length_1877_cov_0.947072, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000509 DBMDQJ010000000
VERSION     DBMDQJ010000509.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20883_length_1877_cov_0.947072"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(68..1018)
                     /locus_tag="ACZO9R_09745"
     CDS             complement(68..1018)
                     /locus_tag="ACZO9R_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003508252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR0913379.1"
                     /translation="MMELDTIFCGDALDVLRTLPDNFVHCCITSPPYYALRDYGVDGQ
                     IGREDTPAQYVARLTEVFSEVRRVLLPSGTLWLNIADTYCGTGSKGDHLDPKNPSGRN
                     GQSVSLAQRVENVKAKDMLGIPWMLAFALRDHGWYLRSDIIWQKANPMPESCKDRPSR
                     CYEHVFLLTKSRSYYYDALAIAEPMAPSSITRYKRGRIGGKYAEEIPGQNKVQGVNQT
                     RSGGYYEDGAVPTVRNKRDVWQINPFPYKGGHFAAFPPKLVETCLLAGCPQDGIVLDP
                     FLGSGTTAAVAKQMGRHYIGIELNPNYCALAEQRIGGVTE"
     gene            complement(1015..1113)
                     /locus_tag="ACZO9R_09750"
                     /pseudo
     CDS             complement(1015..1113)
                     /locus_tag="ACZO9R_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289913.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     assembly_gap    1197..1206
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1207..>1877)
                     /locus_tag="ACZO9R_09755"
     CDS             complement(<1207..>1877)
                     /locus_tag="ACZO9R_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB4-like conjugal transfer ATPase, CD1110
                     family"
                     /protein_id="HIR0913380.1"
                     /translation="GLQPVEKTIIDRCVRMVYRDYLNDPKPENMPVLEDLYQELRRQE
                     EKEAQYVATALEIYVTGSLNVFNHRTNVDIQNRIVAFDIKELGKQLKKIGMLVVQDQV
                     WNRVTVNREAHKATRYYIDEFHLLLKEEQTAAYSVEIWKRFRKWGGVPTGITQNVKDL
                     LASREVENIFENSDYIYMLNQASGDRQILAKQLNISPHQLSYVTHSGEGEGLLFYGNI
                     ILPFV"
BASE COUNT          395 a          530 c          486 g          456 t
ORIGIN      
        1 gtcacgcgga ttttgcgttc cagcggccac gcctgcatga cgcttaaatc ctgccgggtg
       61 tagacgttca ttccgtcaca cctccgatcc gctgctcggc tagcgcgcag tagttcgggt
      121 ttagctcaat gccgatgtaa tgccgcccca tctgctttgc aacagcggcg gtcgtgccgg
      181 agccgagaaa cgggtccaga acgataccgt cctgcggaca accggcaagc aggcacgttt
      241 ccaccagctt gggcggaaat gctgcaaaat gaccgccctt gtagggaaac ggattgatct
      301 gccacacatc gcgtttgttc cgaacagttg gtactgcacc atcttcataa tagccgccgc
      361 tgcgcgtctg attgactcct tgcaccttat tctgtccggg aatttcttcg gcgtatttac
      421 cgccaatgcg tccgcgcttg tagcgcgtga tggaggacgg agccattggc tctgcaatcg
      481 ccaaggcgtc atagtaatag ctgcgtgatt ttgtcagcag gaaaacgtgt tcatagcagc
      541 ggctcggacg gtctttgcag ctctccggca tggggtttgc cttttgccag atgatgtccg
      601 agcgcagata ccagccgtga tctcgcagcg caaacgccag catccacggg ataccgagca
      661 tatcctttgc cttgacattt tccactcgct gcgcaagcga tacactctgt ccgttcctgc
      721 cgcttggatt cttcgggtcc agatggtcgc ctttgctgcc ggtgccgcag taggtatccg
      781 caatgttcag ccagagcgtg ccgcttggaa gaagcacgcg ccgaacctcg gaaaatacct
      841 ccgtcagccg cgccacatac tgcgcaggtg tgtcctcgcg cccgatttgt ccatccacgc
      901 cataatcccg cagcgcgtaa tagggcggcg aggtgatgca gcaatgcaca aagttgtccg
      961 gcagcgtgcg cagcacatcc agtgcgtcgc cgcagaaaat cgtatcaagc tccatcatgc
     1021 gccgctcacc tcgttcggct tggtggtcat cacccgatac agctcggtgt ccttggggaa
     1081 acggtctacg aaaggcaaaa tgatattccc atattcaaaa tgcggattgt gtgactgaaa
     1141 cgcgtctggg aaattctatt ttagttgtat atggctttta taagccgacc acaaccnnnn
     1201 nnnnnntcta cgaaaggcaa aatgatattc ccatagaaca gcaatccctc gccctcgccg
     1261 gagtgggtca catacgagag ctgatgtggg gagatattga gctgcttggc gagaatctgc
     1321 cggtcgccgc tggcttggtt gagcatataa atgtagtcgc tgttttcaaa gatgttttcg
     1381 acctcgcggg aggcaagcag atccttgaca ttttgtgtga taccggtcgg aacaccgccc
     1441 cattttctaa aacgcttcca aatctcaaca ctgtacgcgg cggtttgttc ctcttttaag
     1501 agcaagtgga actcgtcaat ataataccgc gtggccttgt gcgcttcacg gttgaccgtg
     1561 accctgttcc acacctgatc ctgtacgaca agcatcccaa tcttcttgag ctgcttgcca
     1621 agctctttaa tatcaaacgc gacgatgcgg ttctgaatgt ccacgtttgt ccggtgattg
     1681 aacacattca gagagccggt gacgtagatt tccagtgccg tcgccacata ctgcgcttcc
     1741 ttttcctcct gacggcgcag ctcttgataa aggtcctcca gcaccggcat attttctggc
     1801 ttagggtcat tcaggtaatc ccggtagacc atacgcacgc agcggtcgat gatggttttc
     1861 tcgaccggct gtaagcc
//
LOCUS       DBMDQJ010000510         1866 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21047_length_1866_cov_1.137677, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000510 DBMDQJ010000000
VERSION     DBMDQJ010000510.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21047_length_1866_cov_1.137677"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..893
                     /locus_tag="ACZO9R_09760"
     CDS             <1..893
                     /locus_tag="ACZO9R_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HIR0913381.1"
                     /translation="QRDRDRILHSKAFRRLKNKTQVFLTPKGDHYRTRLSHTLEVSQN
                     ARTIAKALRLNEDLVEAIALGHDIGHTPFGHAGEFVLNGLCENGFRHNEQSVRIVEKL
                     EKDGKGLNLTWEVRDGILNHQSRLMPHTLEGKIVRFSDKIAYINHDIDDAIRALVLTE
                     DDIPLELRKTLGFSTKQRLNTLIHNIIMNSREKDDIMMSPEIEEAMIDLRKFMFENVY
                     KNPVAKGEEVKAKAMIEQLFYFYKEHLDQLPAKYLRMMDEGESAERVVADYIAGMTDQ
                     YAITKFSEYFLPQAWQVDGY"
     gene            981..>1866
                     /gene="dnaG"
                     /locus_tag="ACZO9R_09765"
     CDS             981..>1866
                     /gene="dnaG"
                     /locus_tag="ACZO9R_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR0913382.1"
                     /translation="MPRYSDELIEEVRSRNDIVDVISQYVRLSKKGSTYFGLCPFHNE
                     KTGSFSVSPNKQMYYCFGCHAGGNVFTFLMQYENYTFGEAMEALAERAGVDLPKQEYT
                     AAQRQEADRRARLLEINKEAAKYFFVLLRGERGKRALDYFKKRALSDETIHKFGLGYS
                     DQYSDDLYRYLRHVGYDDALLKESGLVSIDEVRGGHDKFWNRAMFPIMDVHNRVIGFG
                     GRVMGEGEPKYLNSPETKIFDKSRNLYGLNIARTTRKPQLLLCEGYMDVIALHQAGFD
                     NAVASLGTSLTSGHASLLK"
BASE COUNT          474 a          409 c          568 g          415 t
ORIGIN      
        1 ttcagcgtga cagagaccgc atcctgcaca gcaaggcgtt ccggcgtctc aagaacaaaa
       61 cgcaggtgtt tctgacgccg aagggcgatc attaccggac gaggctgtcc catacactcg
      121 aggtatcgca gaatgccaga acgatcgcaa aggcactgcg cctcaatgag gatctggtcg
      181 aggcgattgc gctcggacac gatatcgggc atacgccgtt tggacatgcc ggcgagttcg
      241 tgttaaacgg gctctgcgag aatggattcc ggcacaacga acagagtgtg cgtatcgtgg
      301 agaagctgga gaaggatgga aaggggctga atctcacatg ggaagtgcgg gacggtattt
      361 tgaaccatca gtccagactg atgccgcata cgctcgaggg aaagattgtg cgtttttcgg
      421 ataagattgc ctatatcaac cacgatattg acgatgcgat ccgtgcgctg gtcttaacgg
      481 aggacgatat tccgcttgaa ctcagaaaga cccttggttt ttccaccaag cagcgcctca
      541 atacgctgat acataacatt atcatgaaca gcagggagaa ggatgacatc atgatgtcgc
      601 cggagatcga ggaggcgatg atcgatctgc gcaagttcat gtttgagaac gtctacaaga
      661 accctgtggc aaagggagag gaagtcaagg cgaaggcgat gatcgagcag ctcttttact
      721 tttacaagga gcatctggat cagctcccgg caaaatatct gcgcatgatg gacgagggcg
      781 agagcgcgga gcgcgtggtc gcggactaca tcgcaggaat gacggatcag tatgccatca
      841 ccaagttttc ggagtacttt ttgcctcagg cctggcaggt ggacggctat taagactgtg
      901 tcatgcctga ggcgtcccag ggcctggcag gtggacggct attagaatgg aaataaaaag
      961 cgggaagaaa ggagcggaag atgccgcgtt attcggatga attgatagaa gaagtgcgtt
     1021 cgcgcaacga tatcgtggac gtcatttcgc agtatgtgcg gctgtcaaaa aagggcagta
     1081 cctacttcgg gctgtgccct ttccataatg agaagaccgg ttctttttcg gtctctccca
     1141 ataaacagat gtactattgt tttggctgcc acgcgggagg caatgttttt accttcctga
     1201 tgcagtacga gaattacacg ttcggggaag cgatggaagc gcttgccgag cgggcgggcg
     1261 tcgacctccc gaagcaggaa tatacggcgg cgcagcggca ggaggcggac cggagggcac
     1321 gtcttttgga gatcaacaag gaggcggcaa agtatttttt cgtgctgctt cgcggggagc
     1381 gcggaaaacg tgcgcttgat tattttaaaa agagggcgtt gtccgatgag acgatccaca
     1441 agttcgggct gggatattcg gatcagtaca gcgatgatct ctatcgctat ttaagacatg
     1501 tgggatatga cgatgcgctg ctgaaagagt cggggcttgt ctccatcgat gaggtgcgcg
     1561 gaggccatga caaattctgg aatcgtgcca tgtttccgat catggatgta cataaccggg
     1621 tgatcggatt tggagggcgt gtcatgggcg aaggggaacc gaagtatttg aattcgccgg
     1681 agacgaagat ttttgacaag agccggaatc tgtatggatt aaatattgca aggacaacca
     1741 gaaaaccgca gctgctttta tgtgagggat atatggatgt cattgcactg caccaggcag
     1801 gctttgacaa tgcggtggcc tctcttggca catcgcttac cagtggacat gccagtctgc
     1861 tgaaac
//
LOCUS       DBMDQJ010000511         1865 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21061_length_1865_cov_1.348639, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000511 DBMDQJ010000000
VERSION     DBMDQJ010000511.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21061_length_1865_cov_1.348639"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="ACZO9R_09770"
     CDS             <1..204
                     /locus_tag="ACZO9R_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endosialidase"
                     /protein_id="HIR0913383.1"
                     /translation="KATDTGMEFSVEGAEDAQVTVELEDEAEYDIVIDGADAGRMKTN
                     LGGKLSLSVELGDAPVKVSVQKC"
     gene            381..1751
                     /gene="radA"
                     /locus_tag="ACZO9R_09775"
     CDS             381..1751
                     /gene="radA"
                     /locus_tag="ACZO9R_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR0913384.1"
                     /translation="MAKGKTTVYFCQECGYESAKWMGQCPACHEWNSFVEETVEKKSA
                     RGSRSADSSREAKAVPLSQIEMTQEQRVSTGMKELDRVLGGGIVQGSLVLVGGDPGIG
                     KSTLLLQVCRNLAEHKVKVLYISGEESLQQIKIRAERIGTFGDSLSLLCETNLDAIRR
                     VIDREKPEIVVIDSIQTMYNEDVSSAPGSVSQVRESTGTLMQIAKGLGISIFIVGHVT
                     KEGVVAGPRVLEHMVDTVLYFEGDRHAAYRILRGVKNRFGSTNEIGVFEMRGDGLTEV
                     ENPSEFMLSGKPEGASGSVVACSMEGTRPILLEIQALVCHSNFGMPRRTAAGTDFNRV
                     NLLMAVLEKRLGLSLGNCDAYINIAGGIRMNEPAIDLGLVLAIVSSYKDRPIDEKTIC
                     FGEVGLSGEVRAVSMAEQRVLEAKKLGFKTCILPEVCRESLKEIKGIGLVGVRTVKDA
                     MDYVMR"
BASE COUNT          472 a          375 c          612 g          406 t
ORIGIN      
        1 aaagcaacag acacaggaat ggagtttagc gtagaaggcg cagaggacgc acaggttacc
       61 gtagagcttg aggatgaggc tgagtacgac atcgtgatcg acggtgcaga tgccggcagg
      121 atgaagacga atctcggagg aaagttaagc ctgagcgtag agctcggcga tgctccggta
      181 aaggtaagcg ttcagaagtg ctagagcagc cgctttcgca tatgacaatt acaagatcag
      241 tgagacctgt ccgtatggac aggtctttgt tgctctatag gaaatgacgt gttatgttaa
      301 agcgcgaaag ccgcgctttc tatggagaaa aaagccctgt ccgggcagca gccgcgtctg
      361 cgggaaagga agataagaac atggcaaaag gaaaaacaac agtgtacttc tgtcaggagt
      421 gtggatacga gtcggcaaaa tggatgggac agtgtccggc atgccatgag tggaactcgt
      481 ttgtggagga gacagtcgag aaaaagagcg cgcgtgggag ccgcagtgcg gatagcagcc
      541 gtgaggcaaa ggcagttccc ctgtcacaga ttgagatgac gcaggaacag cgggtgtcca
      601 ccggcatgaa ggagcttgac cgggtgcttg gcggcgggat tgtgcaggga tccctggtgc
      661 ttgtgggtgg agatccggga atcggaaaat cgacactgct tttgcaggta tgcaggaatc
      721 ttgcggaaca taaggtgaag gtgctctata tttccggtga ggaatccttg cagcagatca
      781 aaatccgggc agaacgcatc ggaacctttg gggacagctt aagtctgctc tgtgagacca
      841 atcttgatgc catacggcgc gtgattgacc gggagaagcc cgagatcgtc gtgattgact
      901 ccattcagac gatgtataat gaagacgtct cgtctgcacc gggaagcgtc tcgcaggtgc
      961 gggaatcgac cggcaccctg atgcagatcg ctaaaggact tggaatttcc atttttatcg
     1021 tcggacatgt gacaaaggaa ggcgtggtgg ctggtccgcg tgtgctggaa cacatggtgg
     1081 atacggtgct ctattttgag ggggaccgcc atgcggcgta tcgtattctg cgcggtgtga
     1141 agaaccgctt tgggtcgacg aacgagatcg gtgtttttga gatgcgggga gatggtctga
     1201 cagaggtgga gaacccgtcg gaattcatgc tgagcggaaa gccggaggga gcctccgggt
     1261 ctgttgtggc atgctcgatg gaaggaacgc gaccgatcct tctggaaata caggcattgg
     1321 tctgccacag caatttcggt atgccgaggc ggacggcggc aggaaccgat tttaaccggg
     1381 tgaatcttct gatggcggtt ctggaaaagc gtctgggact ttcactggga aattgtgatg
     1441 cctatatcaa tattgcggga ggcatccgga tgaatgagcc ggcaatcgat cttggtcttg
     1501 tgctggcgat tgtctccagt tacaaagacc gcccgatcga tgagaagacc atctgcttcg
     1561 gtgaggtggg actcagcggt gaggtgcgcg cggtcagcat ggcagagcag cgtgtgctcg
     1621 aggcgaagaa gcttggattt aagacatgta ttctgccgga ggtgtgcagg gagagcctaa
     1681 aggagatcaa ggggatcggt cttgtcggcg tgcgcacggt aaaggatgcg atggattacg
     1741 tgatgcggta gtccgtagga agggaaaatg aatggttctg aattgtatgt acaattaaaa
     1801 caattcagaa ccgaaaactt tcttttttaa aacggcggaa atgtggttga cattctaaaa
     1861 taatg
//
LOCUS       DBMDQJ010000512         1864 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21078_length_1864_cov_0.990357, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000512 DBMDQJ010000000
VERSION     DBMDQJ010000512.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21078_length_1864_cov_0.990357"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            82..1503
                     /locus_tag="ACZO9R_09780"
     CDS             82..1503
                     /locus_tag="ACZO9R_09780"
                     /inference="COORDINATES: protein motif:HMM:NF012245.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913385.1"
                     /translation="MVIVWCSIIALSLVSLMGYGFTVLAIRGIAILVVAGIISTIGCY
                     LPIDDAKKVLILVFPPAIGTLLYSWVYGGNSIPYLANFVLLAMTSSYFMESVIIYFAV
                     PFAVISVVFMAFSPETIAGIDYTMAGVVTRIFLFIVTAILLYFATKRGAGVVKKTEET
                     LSIVQNNAKVANTISANLNTTIHKSMSSVHALADGSSSVKSAASQMGQVVEDTANATV
                     SVMDKINAATTEINRNHELAVSLDQGFQKVQSAVEKGNGAIQTAKSSILSMEETVDSA
                     RKSTDSLLTEMNRITSILGEINSIASQTNLLSLNASIEAARAGEHGRGFAVVADEIRA
                     LSEESAKAANNIQEILTWLTDTTGQISKEITAGTDAASASVELVGGLMDYFSNINDAT
                     DEASQIVDEEYKIIEHVKEHFGNIQQEIETLVATSEENSATIQNITDTITSQNDSIRS
                     ISAEIDEISSLSADLEQHFWEDN"
     gene            1605..>1864
                     /locus_tag="ACZO9R_09785"
     CDS             1605..>1864
                     /locus_tag="ACZO9R_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR0913386.1"
                     /translation="MQITDMQEIPAISKDTIALMERAVLEEQPDLVVYTGDQIKGYGV
                     TYKGKGEELEREVAQTIGKLLEPVTKRGIPFAVTFGNHDCQVG"
BASE COUNT          511 a          367 c          522 g          464 t
ORIGIN      
        1 tgatatatat aatagataca tacgcaaaga gaagttttct taagaaggga acatgatatg
       61 gaaaaattgc acaggcagaa tttggttatt gtctggtgct cgattattgc acttagcctt
      121 gtttctttga tgggatacgg atttaccgtg cttgcaatcc gtggaattgc aattctggta
      181 gttgcgggaa ttatttctac gattggatgt taccttccga ttgacgatgc gaaaaaagtg
      241 ctgattcttg tatttccgcc ggcgatcggt acgctgttgt attcgtgggt atatggtggc
      301 aattcgattc cgtatctggc gaattttgta cttttagcga tgacaagttc ctattttatg
      361 gagtcggtta tcatctattt cgcagtgccg tttgccgtta tttcggtcgt attcatggct
      421 ttcagccccg agaccatcgc gggaattgac tacacgatgg ccggggtcgt gacgcgtata
      481 tttttgttta tcgtgacagc gatcctgctt tattttgcga cgaagcgcgg tgcgggtgtt
      541 gtgaagaaaa cggaagagac gctttccatc gttcagaaca atgcgaaagt cgccaatacg
      601 atttcagcga atctgaacac cacgatccac aagagtatga gttcagtgca tgcgcttgcg
      661 gacggcagct ccagcgtgaa atcggcggca tcgcagatgg ggcaggtggt tgaggatact
      721 gctaatgcaa ctgtcagcgt gatggataaa attaatgcgg caacaacaga gattaaccgg
      781 aaccatgaac tggcagtttc tctggatcag ggatttcaga aggtgcagag tgcggtagaa
      841 aaaggaaacg gagcgataca gacggcaaag agttccattc tttccatgga ggagaccgtc
      901 gattccgcgc gcaaatcgac cgattcgctg cttaccgaga tgaaccggat cacatcgatt
      961 ctcggagaga tcaattccat tgcatcccag acgaatctgt tgtcgttaaa tgcatctatt
     1021 gaggcggcgc gtgcgggaga acacggaaga ggatttgcgg tggttgcaga tgagatccgc
     1081 gcgctgtcag aagagagtgc caaggcggcg aacaacattc aggagatttt gacctggctg
     1141 acggatacga ccgggcagat ctcaaaagaa atcacggcgg gcaccgatgc tgcttccgcg
     1201 agtgtggagc tggtcggcgg attgatggat tattttagta acatcaatga tgcgacggat
     1261 gaggcgagcc agattgtcga tgaagaatat aagatcattg aacatgtgaa ggaacacttt
     1321 ggaaatatcc agcaggagat cgagacgctg gtggcaacct cagaggaaaa ttctgcaacc
     1381 atccagaata tcacggacac aatcacgtcg cagaatgatt ccatccgcag catttcggct
     1441 gaaatcgatg agatttcatc attatcggca gatctggaac agcatttttg ggaagataac
     1501 taagaaaagg tcaagggaga catgcagggg cgcagtctcc cttttttaca ccacggagaa
     1561 taaggatgaa attgagattt caaaaagacg gtacttttaa aatcatgcag attacagata
     1621 tgcaggaaat tcccgcaata tcgaaagaca cgatcgcgct gatggagcgg gcggttctgg
     1681 aggagcagcc ggatcttgtc gtctacactg gtgatcagat caagggctat ggtgtgactt
     1741 acaagggaaa aggagaggag ctggagcggg aggtagcaca gacgattgga aaattattag
     1801 agccggtgac aaagcgtggc attccgtttg cagtgacatt tggtaatcat gactgccagg
     1861 tcgg
//
LOCUS       DBMDQJ010000513         1864 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21079_length_1864_cov_0.857629, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000513 DBMDQJ010000000
VERSION     DBMDQJ010000513.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21079_length_1864_cov_0.857629"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="ACZO9R_09790"
     CDS             complement(<1..829)
                     /locus_tag="ACZO9R_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913387.1"
                     /translation="MTKTVQKSKARDMTQGSIWKQLLLFSLPLMAGNLFQQLYNTVDS
                     IVVGNFVGKEALAAVGSVGPIINSLIGFFMGLSTGAGVIISQYYGAKADEKVSRTVST
                     TLVMTFFLSIVFTILGIFITPYMLRMMSTPNDVIRESATYLKIYFGGVAGLMFYNMGS
                     GVLRAVGDSRHPLYFLIFSAVVNTVLDLLFVVSFGMGIEGVALATVIAQCLSAVLTLY
                     VLTTTDGPYRICWKKLCMDWSLLYRIVCVGLPAAIQQMVTSVSNVFVQSYINVFGSDV
                     "
     gene            1129..>1864
                     /locus_tag="ACZO9R_09795"
     CDS             1129..>1864
                     /locus_tag="ACZO9R_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR0913388.1"
                     /translation="MGDYFVCSKTDPVVETKAGKVRGFRLNTTYAFHGIHYAEADRFQ
                     MPQPVKPWKGIKNALAYGYVCPLLKQDEPNMEVLVPHRYWPQDEHCQNLNVWTQSLDP
                     GAKKPVMVWLHGGGFSAGSAIEHVAYEGDHLSEFGDVVVVSVNHRLNILGYLDLSPFG
                     EKYKNSANAGNADMVAALQWVHDNIAAFGGDPENVTIFGQSGGGMKVATLMNTPAADG
                     LFQKGIIESGVYEAKVYQKEDGDGTAI"
BASE COUNT          528 a          402 c          522 g          412 t
ORIGIN      
        1 taacatccga gccgaataca ttgatatagg actgcacgaa aacgttggaa acggaagtca
       61 ccatctgctg gatcgctgcc ggcagtccga cgcagacgat gcggtacagc aaagaccagt
      121 ccatgcagag ctttttccaa cagatgcggt atgggccgtc tgttgtggtc agcacataca
      181 gcgtcagaac cgccgaaagg cactgtgcaa taacggttgc aagcgccaca ccctcgattc
      241 ccatgccgaa ggaaacgaca aacaaaagat cgagcacggt attgacaaca gcggaaaaaa
      301 tcaggaaata cagcggatgg cgggaatcgc cgacagcgcg gagaacgccg gagcccatgt
      361 tgtagaacat cagaccggca acaccgccaa agtaaatttt aagatacgtt gctgactcgc
      421 ggatgacgtc gttcggcgta ctcatcatgc ggagcatgta cggtgtaatg aagatgccga
      481 gaatagtaaa gacaatagag agaaaaaatg tcatcaccag tgtcgtgctg acggtgcggc
      541 tgaccttttc atccgccttt gcgccataat actgagagat aatgacaccg gcaccggtgg
      601 aaagtcccat gaaaaagccg atcagagaat tgatgatcgg accgacggag ccgacggcgg
      661 cgagtgcttc cttgccgaca aagttgccga caacgatgct gtcaacggtg ttgtaaagct
      721 gctggaagag atttccggcc atcagcggaa gcgagaagag aagaagctgt ttccagatag
      781 agccctgggt catatcgcgg gctttgctct tttgtactgt tttcgtcata gtagtcccct
      841 tttttacgtt gattttacct tcttttttat tgtagcactt tgaagttgtg atggaaatat
      901 cagtttttga cgcgattttc ggactttatt gatataaaca gccgaaaata aaagtgaaaa
      961 gaattgtttc tgaaaaaatt gtctggtata ataaggagca gatgcgtaaa tgcaaaaaca
     1021 acagatgctt caaagaccgg tgcaaaaacg gaaagatcag ggggattttg cggtggtttt
     1081 ttggaaactg cgcgtcacag aacaggatag aaagaaggta actgaaatat gggagattat
     1141 tttgtatgca gtaaaacaga tccggtagta gagacaaaag caggaaaggt acgcggtttc
     1201 cgactgaaca cgacgtatgc ctttcatgga atccattacg ccgaggcgga tcgtttccag
     1261 atgccgcagc cggtgaaacc gtggaaagga atcaaaaatg cactggcata cggctatgta
     1321 tgcccgcttt taaagcagga tgagccgaac atggaggtgc tggtaccgca tcgctactgg
     1381 ccgcaggacg agcattgcca gaacctgaat gtctggacac agagtcttga tccgggtgcg
     1441 aaaaagccgg tcatggtatg gctccacggc ggcggcttca gcgccggctc tgcgattgag
     1501 catgtggcat atgaggggga tcacttaagc gagttcggtg atgtcgttgt tgtttccgtg
     1561 aaccatcggc tgaatatcct cggttatctc gatctctcgc cgtttggcga aaaatacaaa
     1621 aattctgcga atgcaggaaa cgcagatatg gtagctgcat tgcagtgggt acatgacaat
     1681 atcgcggcat tcggcggaga cccggagaat gttacgattt tcggtcagtc cggcggcggt
     1741 atgaaggtag caacgctgat gaatacgccg gcagcagacg gcctgttcca gaagggtatc
     1801 atcgagagcg gtgtttatga ggcaaaggtt tatcagaaag aggacggaga cggcaccgcg
     1861 attg
//
LOCUS       DBMDQJ010000514         1863 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21091_length_1863_cov_1.368331, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000514 DBMDQJ010000000
VERSION     DBMDQJ010000514.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21091_length_1863_cov_1.368331"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="ACZO9R_09800"
     CDS             <1..1253
                     /locus_tag="ACZO9R_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HIR0913389.1"
                     /translation="VEQGTMAENNIYRGLILRQEQVYSSDTAGALNVYVKEASRVGYG
                     NLICSVDEGGSVSKKIEEAAGNASNLSAHDLSELEDSISEFQTSYSAQNFYNVSTFKE
                     DLDSALNESLSLAALDGISDYAATAQAENTFHTYHADQPGIVVYSTDGYEGVTTDTFQ
                     SSMFDEASYDRTDGQRALQVAAGAPLYKLITSEYWSLVIPIGSDLAKRLADDDTLQLR
                     FMKDNTTTYATYTITEKEGSTYLILTLRSGMVRYAKDRYAEVELLLSEETGLKIPNSS
                     ITEKEFYTVPKDFFMKGGDSGSLGILVQRSDSSGKAGAEFIAPTIYYETDTDYYIDGE
                     EVGASDIIRKADSTETYQIGSGTASLQGVYNINKGYAIFKQIDILYQNEEYAIVRTGT
                     TYGIALYDHIALDGSKIHENDLIN"
     gene            1269..>1863
                     /locus_tag="ACZO9R_09805"
     CDS             1269..>1863
                     /locus_tag="ACZO9R_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR0913390.1"
                     /translation="MLKENLKTVEEHVQEACKRAGRSREEVTLIAVSKTKPLEMLQEI
                     YGEGVRDFGENKVQELCDKMEQLPSDIRWHMIGHLQRNKVKYIVGKVALIHSVDTYRL
                     AEEINIQAKKRGIIVPILVEVNIAGEETKFGTTAEDAMLLVEEISKLENVRIRGLMTI
                     APFVENPEDNRLYFRKIKQLSVDITNKNIDNVSMEILS"
BASE COUNT          512 a          427 c          505 g          419 t
ORIGIN      
        1 aagtagagca gggcacgatg gcagaaaata atatataccg cggactgatt ctgcgccagg
       61 agcaggtcta ttcctcggat acggcggggg cgctgaatgt atatgtaaag gaagcttccc
      121 gggtgggtta cggaaatctg atctgctccg tcgatgaagg tggcagtgtg tccaagaaaa
      181 tcgaggaggc agccggcaac gcttccaatc tgagtgcgca tgatctctcg gagcttgagg
      241 attccataag cgaatttcag acttcctaca gcgcccagaa tttttataac gtttccacgt
      301 ttaaggaaga tcttgactct gcactgaatg aatccttgag ccttgccgcg ctggacggaa
      361 tctctgatta cgccgcgacg gcgcaggcag aaaatacatt tcacacctat catgccgatc
      421 agcccggcat tgtcgtctat tccaccgacg ggtatgaggg cgttaccacg gatacgtttc
      481 agtcgtccat gtttgacgag gcatcctacg accgcacgga cggtcagcgg gcactgcagg
      541 ttgcggcagg agcccctcta tataagctga ttacaagtga atactggtcg cttgtcattc
      601 caatcggctc cgatctggca aagcggctgg cggacgatga taccttgcag ctgcgtttta
      661 tgaaggataa caccacgacc tatgcgacct ataccatcac ggaaaaggaa ggttccacct
      721 atctgattct gacgcttcgc tcggggatgg tgcgctatgc caaggaccgc tatgccgagg
      781 tggagctgct gcttagtgag gagaccggac ttaagattcc caattcctcc atcacggaga
      841 aggaattcta taccgttccc aaggatttct tcatgaaagg cggcgattcc ggcagtctgg
      901 gcattctggt gcagaggagc gattcctccg ggaaagcagg ggcggagttc attgccccga
      961 ccatctacta tgagacggat accgattatt acatcgacgg tgaggaggtt ggcgcttcgg
     1021 atatcatccg aaaggcggat tctacggaga cgtaccagat cggttccggc acggcgagcc
     1081 tgcagggcgt ttataatatc aataagggat atgccatctt caaacagatc gacattctct
     1141 atcagaatga ggaatacgcc atcgtgcgga ccgggacaac ctacggaatt gcgctctatg
     1201 accatatagc actcgacgga agtaagattc acgaaaatga tctgatcaat taaacggagg
     1261 aaatagatat gttaaaggaa aatttaaaaa cagtggaaga gcatgtacag gaggcttgta
     1321 agcgtgcagg acgttcccgc gaggaagtga cgttgatcgc tgtcagcaaa acaaaacctt
     1381 tggaaatgct gcaggaaatc tacggggagg gcgtccgcga tttcggagaa aataaagtgc
     1441 aggaactgtg cgataaaatg gagcagctcc cgtccgacat ccgctggcac atgatcggcc
     1501 atctgcagcg caacaaggtc aaatatattg tcggaaaggt tgccctgatt cattcggttg
     1561 atacataccg gctggcagaa gagatcaata tccaggcaaa gaaacgcggc atcatcgtgc
     1621 cgattctggt ggaagtcaat atcgccggcg aggagaccaa attcggcaca acggcagaag
     1681 atgccatgtt gcttgtggaa gagatttcca aactggaaaa tgtgcggatc aggggactta
     1741 tgacgattgc gccgttcgtt gaaaatccgg aggataaccg cctttatttt cggaaaataa
     1801 agcaattatc tgttgacata acaaataaaa acatagataa tgtatctatg gaaattctgt
     1861 cta
//
LOCUS       DBMDQJ010000515         1859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21139_length_1859_cov_1.602958, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000515 DBMDQJ010000000
VERSION     DBMDQJ010000515.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21139_length_1859_cov_1.602958"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="ACZO9R_09810"
     CDS             <1..203
                     /locus_tag="ACZO9R_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HIR0913391.1"
                     /translation="LYDASSDQVFLGDPADPDGNRSWIPLRYVYDALKTVSQYQYLLV
                     QDYAEEQNQWMHDGITDAWGGM"
     gene            302..832
                     /locus_tag="ACZO9R_09815"
     CDS             302..832
                     /locus_tag="ACZO9R_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913392.1"
                     /translation="MKENVAYLLGIVAGVAAVGIICWVKKRGRRETDYDERQYQTRDR
                     ACRYSFLTLLLYELVYGALYMREAPSWCDYTMGNYLGIGLALLVFGVYCIWNDAYMNL
                     NQKPWSVHLMFGVLALCNLGIAENNQRIGTLFADGKLGFGAVNLVFAVMFIVFEIVFF
                     IKRSREKRDLSEEKAE"
     gene            829..1032
                     /locus_tag="ACZO9R_09820"
     CDS             829..1032
                     /locus_tag="ACZO9R_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0913393.1"
                     /translation="MKNLRLKAARAAKDLSQQTLAEKVGVSRQTINAIEKGDYNPTIR
                     LCIAICRELDKTLDEIFWEEEEC"
     gene            1026..>1859
                     /locus_tag="ACZO9R_09825"
     CDS             1026..>1859
                     /locus_tag="ACZO9R_09825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0913394.1"
                     /translation="MLNLEYILIGKLPKTERDILLENLKTEREYGWNVLEWDSAKEHI
                     QEGALAEKAILVSEDAKELSEAQRIGMAALCYLMPEDGRTQREETAGEMEVTPPADMY
                     AEGMEEIDGSFLQHVYERHHHIPWIILKTPRCIVKEFSMEYLDALFELYAGKGMTDYM
                     EPLYPYEEEREYQQAYIEQMYRFYGYGMWIVCDRNTGELIGRAGVEHREELGGELELG
                     YAIGVPWQRQGYATEVCSAILTYVKEELMLPSVSCLIEEGNVVSEHLAQKLGFHYCET
                     VQ"
BASE COUNT          498 a          333 c          608 g          420 t
ORIGIN      
        1 gcctttacga tgcgtcgtcc gatcaggtgt tcctcgggga tccggcggat ccggacggca
       61 accgctcctg gattccgctg cgctacgtgt atgacgcatt aaaaaccgtc agccagtatc
      121 agtatcttct ggtacaggat tatgcggagg aacagaatca gtggatgcat gacgggatta
      181 cggatgcatg gggaggaatg taatatatat ttgacatatt gtaaagcaga tgctaccata
      241 aaagaagaaa tcagaaacta tcaggattgt gtgacaggaa atgaaacagg aggattggaa
      301 gatgaaagaa aatgtggcgt atctgcttgg gattgtggca ggggtggcag cggtaggaat
      361 tatctgctgg gtgaagaaac ggggacgcag ggagactgac tatgatgagc ggcagtatca
      421 gacgcgcgac cgtgcatgcc ggtacagttt tctgacactg ctgttgtatg aactggtata
      481 tggggcattg tatatgaggg aggcaccgtc ctggtgcgac tatacgatgg gaaattatct
      541 gggaattggt ctggcactgc ttgtgttcgg cgtatattgt atctggaacg atgcttatat
      601 gaacctgaac cagaagccgt ggagtgtaca tctgatgttt ggagtgctgg cactgtgcaa
      661 tcttggaatc gcggaaaata accagcggat cggaacgctg tttgcggacg gaaaactggg
      721 atttggcgcg gtgaatctgg tgtttgcagt gatgtttatt gtctttgaaa ttgttttttt
      781 tataaaaaga agccgggaga agcgggatct gtcggaggaa aaagctgaat gaaaaatctc
      841 aggttaaagg cggcgcgcgc ggcaaaggat ctgtcccagc agacgctcgc cgagaaggtg
      901 ggagtgtcga gacagacgat caatgccatc gagaagggag attataatcc cacaatccgg
      961 ctgtgcattg cgatatgcag ggagctggac aaaacactgg atgaaatttt ctgggaagag
     1021 gaagaatgtt aaatttagaa tatattttaa ttggaaagct gccaaagaca gagcgggaca
     1081 tattgctgga gaatttgaaa acggagcggg aatacgggtg gaacgttttg gaatgggaca
     1141 gcgcgaagga acacattcag gagggggcgc ttgcggaaaa ggcaattttg gtctcggaag
     1201 atgcgaagga actaagcgag gcacagcgga tcgggatggc tgcgctttgc tatctgatgc
     1261 cggaggatgg aaggacgcag agagaagaga ccgccgggga gatggaagtg acgcctccgg
     1321 cggatatgta tgcggaaggg atggaggaga tcgacggatc ttttttacag cacgtttatg
     1381 agagacatca tcatattccg tggatcattt taaaaacgcc gcgctgcatc gtgaaggaat
     1441 tttccatgga atacttagat gcactgtttg aactgtatgc cgggaaagga atgacggact
     1501 acatggagcc gctgtatccg tatgaggagg agcgggaata tcagcaggcg tatatcgagc
     1561 agatgtaccg gttctacgga tacggcatgt ggatcgtgtg cgacaggaat accggagagc
     1621 tgatcgggcg cgccggcgtg gaacaccggg aggagctggg cggagagctg gagctcggtt
     1681 atgcgatcgg tgtgccgtgg cagagacagg gctatgcgac ggaggtatgc agtgcgattc
     1741 ttacctatgt aaaggaggaa ctcatgctgc cgtcggtcag ttgtctgatc gaggagggga
     1801 acgtagtatc ggagcatctg gcgcaaaagc tgggctttca ttactgtgaa acggtgcag
//
LOCUS       DBMDQJ010000516         1859 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21146_length_1859_cov_1.322526, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000516 DBMDQJ010000000
VERSION     DBMDQJ010000516.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21146_length_1859_cov_1.322526"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            176..1012
                     /locus_tag="ACZO9R_09830"
     CDS             176..1012
                     /locus_tag="ACZO9R_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014376.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0913395.1"
                     /translation="MNDFASSSCHDRIADFKITQVQRDSHFDMGESHYHPYYEFYYLL
                     SGQCRCFISHSIYRLFPGDLIMIPPSVLHRVLYETKRPAERINIFFSEDYLHRVSAAS
                     GPDLCVGLFSRSRLTLPSSSRPQLERLMTHLRLESESSDPYAPLMTRSLFSELLVEIA
                     RCQDVHQTPQFLDETSTAIQESARYIYEHYEEPVSLAAAAAVAHMNPTYFSKKFRETT
                     GFGFKEYLTHIRLQNAAHLLRTTDTSITEIAGICGFSDGNYFGDAFRKHYGMSPRAYR
                     LT"
     gene            complement(1157..>1859)
                     /locus_tag="ACZO9R_09835"
     CDS             complement(1157..>1859)
                     /locus_tag="ACZO9R_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014081214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913396.1"
                     /translation="DKATNRVIIDKKEALNLLQRLNVCIYELMEEHELTQQSRDAAER
                     QFRKKTDGIVEDANRMAEDVYAGSVMYTDEALHRVQDIMQDAMDSVREICDKMNDALA
                     QEKSNVRRDQSELKSHLEDLRDTNKYMNLIEQRNKEIAKERAKREQEEEEATSVYASV
                     KPEIKINEEYFEKNGIPLETEEPEEEPEKNTGETGAEIKVNLDAEYFKWKEDADGGKE
                     EKKPERHSFFGKRPK"
BASE COUNT          386 a          550 c          382 g          541 t
ORIGIN      
        1 gtatctgcgt ttctttactt ttctgttctc ccatggtata acaaaaatat atttatttat
       61 acgaattttt tccgttattt tgctttatac tactgatttt ttgtcattgc aggcaaaaat
      121 ccagaatcac cattccgcag aaaccttcac ggaaccagga acaggagtta tacccatgaa
      181 cgatttcgct tcctccagct gtcacgaccg cattgccgat tttaaaatta cgcaggtaca
      241 gcgcgattcg cattttgata tgggagaatc ccattatcac ccgtactacg aattttatta
      301 cctgctgtcc ggtcagtgcc gctgttttat cagccacagc atttaccggc tctttccagg
      361 ggatctgatt atgattcctc cctctgtgct gcaccgcgtt ctctatgaaa ccaagcgtcc
      421 tgccgagcgc atcaacatct ttttttcgga agattactta caccgggtgt ctgccgccag
      481 cggtccggat ctttgcgtcg ggcttttttc ccgcagcaga ctcacccttc cgtcttccag
      541 ccgtccgcag ttagaacgcc tgatgacgca cctgcgcctt gaatccgaaa gcagcgatcc
      601 ttatgcaccg ctcatgacca gaagtctttt ttcagagctt ctggttgaga tcgcgcgctg
      661 ccaggatgtc caccagactc cgcaatttct ggacgagacc agcaccgcca tccaggaaag
      721 cgcgcgctac atctacgaac attacgagga accggtctca ctggcagccg ccgcggcggt
      781 cgcacatatg aatcccactt atttttccaa aaaattccgg gagacaaccg gattcggatt
      841 taaggaatac ttaacgcaca tccgccttca gaacgccgcg caccttctgc gcacgacgga
      901 cacttcgatc actgaaattg ccgggatctg cggtttttcc gacggcaatt atttcgggga
      961 cgcgttccgg aaacactatg gcatgtcgcc gcgcgcctac cggctgacgt aacaaaacgc
     1021 ctgagtcccc caagcacctc gtgagatgca atataaggga actcaggcgt ataacccggc
     1081 aatacttctt ccttgcttcc tgtatgaaat tgaatggtgg aaatatcttt ctccggatct
     1141 gctccttttg attctcttac ttcggccgct tgccaaagaa agaatggcgc tccggtttct
     1201 tctcctcttt cccgccgtca gcgtcttcct tccatttaaa gtattccgca tcaagattta
     1261 cttttatctc cgccccggtc tcaccggtgt tcttctccgg ctcttcctcc ggttcctctg
     1321 tctctaacgg aatcccgttc ttctcaaaat actcttcgtt tatcttgatc tccggcttta
     1381 cggatgcata gacggaagtc gcctcttctt cctcctgctc acgcttcgcg cgttcctttg
     1441 caatctcctt gttgcgctgc tcgatcagat tcatgtattt gttcgtatcc cttaaatcct
     1501 caaggtggct ctttaactcc gactgatctc tgcgcacatt tgatttttcc tgcgcaagcg
     1561 catcattcat cttatcgcag atctcccgga cagagtccat cgcatcctgc atgatatcct
     1621 gtacgcggtg aagcgcctca tccgtataca tgaccgatcc ggcatacaca tcctccgcca
     1681 tgcggttcgc atcctcgacg attccgtccg tcttcttgcg gaactgtcgt tccgctgcat
     1741 cgcggctctg ctgggtaagc tcatgttcct ccatgagttc gtagatgcag acattcaggc
     1801 gctgcaggag atttaacgcc tcctttttgt ctatgataac ccgatttgtc gctttatca
//
LOCUS       DBMDQJ010000517         1858 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21158_length_1858_cov_1.238475, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000517 DBMDQJ010000000
VERSION     DBMDQJ010000517.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21158_length_1858_cov_1.238475"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(41..1168)
                     /locus_tag="ACZO9R_09840"
     CDS             complement(41..1168)
                     /locus_tag="ACZO9R_09840"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HIR0913397.1"
                     /translation="MANMMDYLDWRGDLTFTASGFNEVDNLLFAELVYTSFDGIVTGQ
                     SEAEAVTLAEASAVFWEQNSREEILARVSMTKAAPFVLEKMAKTERFRDVKLWGYVND
                     ISEEEQSQFAVMCAGLPDGSIYVSFRGTDNTITGWREDFNMGYLSETPGQLKAVSYVE
                     QMLGDGSCPVRIGGHSKGGNLAVYAAVHCSSGLQDRILAVYSNDGPGFRRDIVESAAY
                     QRVLPKILTILPESSIVGMLLEHQEAYEVVRSSNSGIQQHDAMSWEVLGTSFVYVREV
                     AAQSLMLDETMKAWIYQLNGEEREQIVDTVFGMLEAADIKTVDDFYHSKWKAVQELMK
                     AKSRLPEKSQKVFAKALKLLWSEGNKTVRKTVKQVVGEKRR"
     gene            complement(1209..>1858)
                     /locus_tag="ACZO9R_09845"
     CDS             complement(1209..>1858)
                     /locus_tag="ACZO9R_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HIR0913398.1"
                     /translation="RSNIRSEKILGIGFDGIVAACGTHIEYEGKKVCERLLTPEEAEE
                     LLRVIEAYHMEAVLEGPHYIYANGEDFKEDPFIQYLRSELGEDVREIRGQTEYEINKL
                     SAYMNGASVRAVEAELVPHFDVIEHNPQLIEVVPKGYTKATGIAQVCDMLGIARENTY
                     AFGDSTNDLEMLTYVGHGIAMGNGAPAAKAAAEYVTTDIMNHGIKNGLKHYELIG"
BASE COUNT          382 a          625 c          370 g          481 t
ORIGIN      
        1 tttcatccat tttttttgat tttatacttt ttttagaaac tcaccgtcgc ttctctccaa
       61 cgacctgttt caccgttttg cggacagttt tatttccctc cgaccacagg agttttaagg
      121 cttttgcaaa gaccttctgg ctcttctccg gcagacggct tttcgccttc atcagctcct
      181 gcaccgcctt ccatttgctg tggtaaaaat catccaccgt cttaatgtcc gccgcttcaa
      241 gcatgccgaa taccgtatcc acaatctgtt cgcgttcctc cccgttcagc tggtaaatcc
      301 atgccttcat cgtctcatcc aacatcaggc tctgggccgc cacctccctg acatagacaa
      361 aggatgtgcc gagcacctcc cacgacatgg catcgtgctg ctggattcca ctgttggaac
      421 tgcgcaccac ctcatacgcc tcctgatgct ctaagagcat tccgacaatc gaagattccg
      481 gcagaatcgt gaggattttc ggcagtacgc gctggtatgc cgcgctctct acaatatccc
      541 tgcggaaccc aggtccgtca ttgctgtaca ccgcaagaat cctgtcctgc agcccgctgc
      601 tgcagtggac tgcagcatag accgcaagat ttccgccctt ggagtgaccc ccgatgcgca
      661 cggggcagct gccatctcca agcatctgtt ccacgtacga aacggctttc agctgaccgg
      721 gcgtctccga gaggtatccc atattaaaat cctctctcca ccctgtgatc gtattgtctg
      781 tgccgcggaa cgacacatag atactgccat ccggcagccc ggcgcacatg accgcaaact
      841 gtgactgctc ctcctcactg atgtcattga cgtaacccca gagctttaca tcgcggaacc
      901 gctctgtttt cgccatcttc tccagaacaa acggcgccgc ctttgtcatt gacacccgcg
      961 caaggatctc ctccctgctg ttctgctccc agaacacggc ggatgcctcc gcaagcgtga
     1021 ctgcctccgc ctcgctctgc ccggtcacaa tcccgtcaaa cgaagtatat accagctccg
     1081 caaaaagcag attatccacc tcgttaaatc cggacgcggt gaaagtcaga tccccccgcc
     1141 agtccagata atccatcata tttgccatcc cgctcctcct tctcttcccg ctgcaatccc
     1201 gttgcttcct atccgatcag ctcatagtgc ttcagtccgt ttttaattcc atgattcata
     1261 atatcggtcg tcacatactc cgcggcagcc ttcgccgcag gtgccccgtt tcccatggcg
     1321 atgccatgtc ccacataggt gagcatctcc agatcattcg tgctgtcgcc gaacgcataa
     1381 gtattctccc tggcaatccc aagcatgtca catacctgcg ctattccggt cgcctttgtg
     1441 tagcccttcg gcaccacttc aatcagctgc ggattgtgct caatcacatc aaaatgcggc
     1501 accagttccg cttccaccgc acgcacgctc gccccgttca tataagcgct caatttgttg
     1561 atctcatact ccgtctgccc acggatctcc cgtacatcct ctcccagttc actgcgcaga
     1621 tactgaataa acggatcttc cttaaaatct tccccgtttg cgtagatata atgcggtccc
     1681 tccagcaccg cttccatgtg gtatgcctca atgacacgta gaagttcctc tgcttcttca
     1741 ggcgtaagca gcctctcgca caccttcttt ccttcatatt caatatgtgt gccgcacgcc
     1801 gccacaatcc cgtcaaaccc tattccaagg atcttctcac tgcggatgtt cgaccggc
//
LOCUS       DBMDQJ010000518         1857 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21169_length_1857_cov_1.257973, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000518 DBMDQJ010000000
VERSION     DBMDQJ010000518.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21169_length_1857_cov_1.257973"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /gene="nusA"
                     /locus_tag="ACZO9R_09850"
     CDS             <1..1167
                     /gene="nusA"
                     /locus_tag="ACZO9R_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR0913399.1"
                     /translation="LEAIENSLVTACKNHFGTSENVKVTMDPQTCEYHVFQEKTVVEQ
                     VEDPVEQISLVNAKMVDSKYEIGDIVNVEIQSKEFGRIATQNAKNVILQKIREEERKV
                     LYNQYYSMEKDVVTGVVQRYIGRNVSVNLGKVDAILTENEQVRGEVFQPTERIKVYIL
                     EVKDTSKGPKILVSRTHPELVKRLFESEVTEVREGIVEIKAIAREAGSRTKIAVWSND
                     PDVDPVGACVGMNGARVNAIVNELRGEKIDIITWNKNPAMMIENALSPAKVISVIADA
                     EEKSAKVIVPDYQLSLAIGKEGQNARLAARLTGFKIDIKSETQARESGDFMDYESEYE
                     DDEYYEDDEYAEDGEYTEDGEYAGDRYAEDADAEGEYAEESELADETDTENEEE"
     gene            1186..1476
                     /gene="rnpM"
                     /locus_tag="ACZO9R_09855"
                     /gene_synonym="ylxR"
     CDS             1186..1476
                     /gene="rnpM"
                     /locus_tag="ACZO9R_09855"
                     /gene_synonym="ylxR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase P modulator RnpM"
                     /protein_id="HIR0913400.1"
                     /translation="MANKKIPMRQCVGCRELKPKKDMIRVIKTAAEDEASSQIVLDAT
                     GRKNGRGAYVCPNSECLRAAIKNKGLERSLKMPIPKEVYDTLTKEMEKFETR"
     gene            1463..1783
                     /locus_tag="ACZO9R_09860"
     CDS             1463..1783
                     /locus_tag="ACZO9R_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L7Ae/L30e/S12e/Gadd45 family ribosomal protein"
                     /protein_id="HIR0913401.1"
                     /translation="MKPDKVLSMLGIAAKAGGVASGEFSTEKAVKDGRAWLVIVASDA
                     SENTQKHFRDMTTFYEVPMYVYGEKETLGHFIGKEFRASLAVTNEGLAHSIEDKLKLT
                     LTTE"
     gene            1801..>1857
                     /locus_tag="ACZO9R_09865"
     CDS             1801..>1857
                     /locus_tag="ACZO9R_09865"
                     /inference="COORDINATES: protein motif:HMM:NF016635.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HIR0913402.1"
                     /translation="MAKMRVHELAKELGIENKQ"
BASE COUNT          567 a          354 c          575 g          361 t
ORIGIN      
        1 cttgaggcaa tcgagaattc actggtgacc gcctgcaaga accatttcgg tacatccgag
       61 aatgtaaagg tgacgatgga cccgcagacc tgtgagtatc atgtgttcca ggagaagacc
      121 gtagtagagc aggtggagga tccggtagag cagatcagcc ttgtcaatgc caagatggtg
      181 gattccaaat acgagatcgg agatatcgtg aatgtcgaga tccagtccaa ggagttcgga
      241 cgcatcgcaa cacagaatgc caagaatgtc atcttacaga agatccgtga agaggagcgc
      301 aaggttttat acaaccagta ttacagcatg gaaaaggatg ttgtgaccgg tgtggtacag
      361 cgttacatcg gaagaaatgt gagcgtcaac cttggcaagg tggatgcgat cctgaccgag
      421 aacgagcagg tgagaggcga ggtattccag ccgaccgagc gcatcaaggt ctatatcctg
      481 gaggtgaagg atacgtccaa gggaccgaag attctcgtgt caagaaccca tccggagctg
      541 gtaaagcgcc tgttcgagtc tgaagtgacc gaggtgagag agggaatcgt tgagattaaa
      601 gcgatcgcgc gggaggcagg aagcagaacc aagattgccg tctggtccaa tgatccggat
      661 gtggatccgg tgggcgcatg tgtcggtatg aacggtgcga gagtcaatgc gatcgtcaac
      721 gaactgcgcg gtgagaagat tgacatcatt acctggaata agaatccggc aatgatgatc
      781 gagaacgcat tgagcccggc aaaggttatt tctgttatcg cggatgcgga ggagaagtcg
      841 gcgaaagtca tcgttccgga ttatcagctt tccctggcga tcggcaagga aggacagaat
      901 gcgagacttg cggcaagact taccggcttt aagatcgaca tcaagagcga gacacaggcc
      961 agagagtccg gcgacttcat ggattatgag agcgagtacg aggacgacga gtattacgag
     1021 gatgacgagt acgcagagga cggcgaatat accgaagacg gcgagtatgc cggggacaga
     1081 tatgctgagg atgcggatgc cgagggagaa tacgctgagg aatccgaact tgcggatgag
     1141 acagacacag agaatgaaga agagtaggca ggggttgatc agagaatggc aaacaaaaag
     1201 attccaatgc gccagtgcgt ggggtgcaga gagcttaagc cgaaaaaaga tatgatccgg
     1261 gtaatcaaga cggcggcgga agatgaggcc tcctcacaga ttgtacttga tgctacgggg
     1321 cggaaaaacg gcagaggcgc ttatgtgtgt ccgaacagtg agtgcctgcg cgcggcaata
     1381 aaaaacaagg gtctggaacg atcattaaaa atgccaattc caaaggaagt ctatgataca
     1441 ctgacaaagg agatggagaa gtttgaaacc agataaagtg ttgtcgatgc ttggcattgc
     1501 agccaaggcg ggcggtgtgg caagcggaga gttttccaca gaaaaagcgg tgaaggatgg
     1561 cagggcatgg cttgtcattg tcgcgtcgga tgcgtctgag aatacacaga agcatttccg
     1621 ggatatgacg actttttatg aagttccgat gtatgtgtac ggagaaaaag agacattggg
     1681 acatttcatt ggaaaggaat tccgtgcatc gcttgcagtg acaaacgaag ggctggccca
     1741 ttcgatcgaa gataaattga aactgacgtt aacaactgaa taatggagga aacaggatat
     1801 atggcaaaaa tgagagttca tgaacttgca aaagagttag gaatagaaaa caaacag
//
LOCUS       DBMDQJ010000519         1852 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21229_length_1852_cov_1.376927, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000519 DBMDQJ010000000
VERSION     DBMDQJ010000519.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21229_length_1852_cov_1.376927"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            172..744
                     /locus_tag="ACZO9R_09870"
     CDS             172..744
                     /locus_tag="ACZO9R_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HIR0913403.1"
                     /translation="MTNQNIKKLVTAALLAAMTCVATMIIKIPTPTFGYIHPGDGFVL
                     LCGVVLGPLPGALAAGIGSMFSDIFSGYVTWAPATFIIKALTGGIAGLLFHRFKNTLH
                     SKKGRYAALAIGGIVGEAIMVIGYFLYETGLAALGSGALNQASIAAGIASSATGIPFN
                     IVQGVVGIILSLILLPVLSKIPDVRDWIER"
     gene            complement(741..>1852)
                     /gene="nagA"
                     /locus_tag="ACZO9R_09875"
     CDS             complement(741..>1852)
                     /gene="nagA"
                     /locus_tag="ACZO9R_09875"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HIR0913404.1"
                     /translation="HGFEERDVRIKNGCFAGSEAAGTDGEEILDAGGDYLIPGLVDIH
                     FHGCAGHDFSDGTPEALQAIGAYELRQGVTSICPASMTLSEETLKTVCANAYAYTKRP
                     EAEQDTGARLIGIHLEGPFISMEKKGAQNPAYIRNPDVEMFLRLQEAAHGLVRLITIA
                     PETDGAKEFIRRLADQVHISVGHTCSDYETAYRAFSQGADHVTHFFNAMPPFTHRAPG
                     VFGAAFDAKHVMPELICDGIHIDASAVRVVFSLFGAERMILISDSMMATGMEDGAYSL
                     GGLPVTVRGNLATLEDGTIAGSATNLMDCMRKAVSMGIPLETAVRAATYNPARAIGAA
                     DVCGSITAGHRGDCVLLSKDDLSTKKVIFGGKVVA"
BASE COUNT          399 a          575 c          446 g          432 t
ORIGIN      
        1 agtgtatcag tgctttgtgt ttcaggcaag aaaaaagacc attccggcaa actggcatgg
       61 tcaatttaat cgcaactgga catttttatc aatccatctt gtgttatact cttatgcatc
      121 aacgttgaaa tacaaacttt tatatgaatc tgacagaatg gagacttcct aatgacaaat
      181 caaaatatta aaaaactggt aactgctgcc cttctcgccg ccatgacatg cgtggcgacc
      241 atgatcatca agatcccgac accaacgttc ggctacatcc atccgggaga tggcttcgtt
      301 ctgctgtgtg gcgtcgtgct tggcccactt cccggagcgc tcgctgccgg catcggctcg
      361 atgttctcag acattttctc cggctacgtg acctgggctc ctgcaacttt cattatcaag
      421 gcgctcacgg gcggcattgc cggactgctc ttccatcgtt ttaaaaacac cctgcactcg
      481 aaaaagggac gctacgcagc gctcgcaatc ggcggcatcg tcggagaagc catcatggtc
      541 atcggctatt ttctctacga aaccggactt gccgcactcg gcagcggagc cttaaaccag
      601 gcatctatcg ccgccggcat tgcttcctct gcgaccggta tcccgtttaa catcgtacag
      661 ggtgttgtcg gcattattct tagtctgata ctgcttccgg tgctctctaa gatcccggat
      721 gtccgtgact ggatcgagcg ctaggcgacc acctttcctc cgaagatgac cttcttcgtg
      781 gacaggtcat ccttggagag aaggacacag tcgccgcggt ggcctgcggt aatgcttccg
      841 cacacatccg ccgcaccgat cgctctcgcc ggattatagg ttgcagcgcg cacggctgtc
      901 tctaacggga ttcccatgga gacagccttt cgcatgcagt ccatgagatt cgtcgccgag
      961 ccggcaatcg ttccgtcctc taaggttgca agattgccgc gcacggtaac cggaagtccg
     1021 ccgagggaat acgctccgtc ctccataccg gtcgccatca tgctgtcgct gattaggatc
     1081 atgcgctccg ccccgaacag ggagaatacc acgcggacag ccgaagcatc aatatgaatc
     1141 ccgtcgcaga tcagctccgg catgacatgc ttcgcgtcaa acgccgcgcc aaacacgccc
     1201 ggcgcgcgat gggtgaacgg cggcatcgca ttgaaaaaat gcgtcacatg atccgccccc
     1261 tgtgaaaatg cgcggtatgc agtctcgtaa tcactgcagg tatgtccgac cgagatatgt
     1321 acttgatccg ccagccggcg gatgaactcc ttggcaccgt ccgtctccgg tgcaatcgta
     1381 atcagacgca ccagcccgtg tgctgcctcc tgcagccgca ggaacatctc tacatccgga
     1441 ttccggatat aagccggatt ctgcgcaccc tttttctcca tggaaataaa cggtccttcc
     1501 agatgaatcc cgatcagtct cgccccggta tcctgctctg cctccggtct ctttgtatac
     1561 gcatacgcat tggcacacac cgtcttaagc gtctcctccg agagtgtcat cgatgcaggg
     1621 cagatgcttg tcacgccctg gcgaagctca tacgcgccga tcgcctgcaa tgcctccggt
     1681 gttccgtccg agaaatcatg acctgcacat ccgtgaaaat gaatgtccac aagccccggt
     1741 atcaggtaat ctccccctgc gtccaggatc tcttccccgt ccgtgccggc cgcttcactc
     1801 cccgcaaaac agccgttttt tatccggaca tcccgctcct caaatccatg ct
//
LOCUS       DBMDQJ010000520         1850 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21257_length_1850_cov_1.823328, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000520 DBMDQJ010000000
VERSION     DBMDQJ010000520.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1850)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1850)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1850
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21257_length_1850_cov_1.823328"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="ACZO9R_09880"
     CDS             <1..203
                     /locus_tag="ACZO9R_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913405.1"
                     /translation="KRKSDDLDDDLFDDDDAYEDDDAYGDDDDTYGDSDAPDDEKEKV
                     SGGKDQKNKQDDELDIIDFNDL"
     gene            242..>1850
                     /locus_tag="ACZO9R_09885"
     CDS             242..>1850
                     /locus_tag="ACZO9R_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR0913406.1"
                     /translation="MQTAYTAKAKKAIDIATRISKSLHHSYIGTEHILLGLLKEGTGV
                     ASQVLADNGVEYDKVLELIEELIAPGNAVAVLEDGLSPRAAHVLEVSKAEAARFHSEK
                     IGTEHLLIAMIKETECVASRLLNTLSVNVQKMYVDTLIAMGEDVSQYKDEFQNGKPGK
                     RKNAEGTPSLDQFSRDLTELARDGKLDPVVGRETEIDRVIQILSRRSKNNPCLIGEPG
                     VGKTAIVEGIAERIMGGMVPDTVLGKRVVSLDLSGIVAGSKYRGEFEERIKKVLAEVA
                     KAGNVLLFIDEIHTIIGAGGAEGAIDASNILKPALARGEVQVIGATTIEEYRKYIEKD
                     AALERRFQPVVVEEPTEEEAISILKGLRGQYESHHHVTITDEAVEAAVRLSARYINDR
                     FLPDKAIDLMDEAAAKVRLHVGGDPREAAELRREIAESQETLEQALSGGDLEAAREAQ
                     TKRQELEEKLEKLNAKAKQGGRRHHQTVGEDEIADVVSGWTKIPVKKLTEGEAARLKK
                     LEAALHKRVIGQEEAVSAVAKAVRRGRV"
BASE COUNT          509 a          377 c          623 g          341 t
ORIGIN      
        1 ggaagcggaa gtctgacgat ctggatgacg atctgtttga tgatgacgac gcgtacgagg
       61 atgatgacgc gtacggggat gacgatgaca cgtacgggga cagcgatgcg ccggacgatg
      121 aaaaagaaaa agtttcaggc ggcaaagacc agaaaaacaa gcaagacgat gagctggata
      181 tcatagattt taatgatctg taaagacgaa tgtgatatcg gactggaaat aggaggtagc
      241 gatgcagaca gcatatacag caaaggcaaa aaaggcgatt gatattgcaa cgcggatatc
      301 aaagtcattg catcacagtt atatcggcac ggagcacata ctgcttggcc tgttgaaaga
      361 gggaaccggt gtggcaagcc aggtacttgc cgacaatggt gtagagtatg ataaggtgct
      421 ggaactgatc gaggagctga ttgcaccggg gaatgcggtt gcggttctgg aggacggatt
      481 gtccccgaga gcggcgcatg tgcttgaggt atccaaagcg gaggcggcgc gtttccactc
      541 ggagaagatt ggaacggaac accttttaat tgcgatgatc aaggagacgg aatgtgtggc
      601 gtccagactg ttgaatacat tgtctgtcaa tgtgcagaaa atgtatgtgg acaccctgat
      661 cgcgatgggc gaggatgtga gccagtataa ggatgaattc cagaacggga aacccggcaa
      721 gcggaaaaac gcggagggaa cgccgtcact ggatcagttt tccagagatc tgaccgaact
      781 tgcacgcgac ggaaagcttg atccggtggt cggcagagag acggagatcg accgtgtcat
      841 ccagatttta agccggagaa gcaagaacaa tccgtgcctg atcggagaac ccggcgtagg
      901 aaaaacggcg attgtagagg gaattgcaga gcgcattatg ggcggaatgg ttccggatac
      961 ggtgcttgga aaaagagtgg tctccctgga tctttccggg attgtggcag gctcgaagta
     1021 caggggtgaa tttgaggagc gcatcaagaa ggtgcttgca gaggtggcga aagccggaaa
     1081 cgtcctgctg ttcatcgatg agattcatac catcatcgga gcgggcggag cggaaggcgc
     1141 gattgatgca tccaacattt taaaacctgc gcttgcgaga ggtgaggtgc aggtgattgg
     1201 tgcgacgacc atcgaggagt atcgcaaata tatcgaaaaa gatgccgctc tggaacgtcg
     1261 gttccagccg gttgttgtgg aggagccgac ggaggaagaa gccatttcca tattaaaggg
     1321 attgcgcgga cagtatgaga gccaccacca tgtgacgatt acggatgagg ccgtggaggc
     1381 ggcagtgcgt ctttctgcgc gttacatcaa cgaccgtttc ctcccggaca aggcgattga
     1441 cctgatggat gaggccgcgg caaaagtgcg ccttcacgtg ggcggagacc cgagggaggc
     1501 tgcagaactc cggcgggaga ttgcggagtc gcaggagaca ttggagcagg cactttctgg
     1561 cggagacctg gaagcggcga gagaggcaca gacaaagcgt caggaactgg aagaaaagtt
     1621 ggaaaaactg aacgcaaagg caaagcaggg cggcagacgg catcaccaga ccgtcggaga
     1681 ggatgagatt gccgatgttg tgtcgggatg gacgaaaatc ccggtgaaga agcttacaga
     1741 gggggaggct gccaggctta aaaagctgga ggcagctctt cacaagcgcg tgatcggaca
     1801 ggaggaggcg gtatcggcag tggccaaagc cgtgcggaga ggccgcgtcg
//
LOCUS       DBMDQJ010000521         1847 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21292_length_1847_cov_1.653494, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000521 DBMDQJ010000000
VERSION     DBMDQJ010000521.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21292_length_1847_cov_1.653494"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1422
                     /locus_tag="ACZO9R_09890"
     CDS             <1..1422
                     /locus_tag="ACZO9R_09890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR0913407.1"
                     /translation="FDSNQMQSEFSELTFFSVHQCVEVYADGEQIYSLEKSSTVFGRT
                     PGTGWNTIQLQEGVKSFSVRIEAVYPAVRDHVTRFYQENDGQMVYQTMRGSVPEIMVS
                     TVDGAIGIMLLIYYAIARRKVKMGPGILYFGIFTLMMGMWSMNEAELVAYMVKSRTAA
                     SYTGYMMIMLMIAPFAAFLQEFSEVEEKYISNIICICSFANAIVCTVLHMTGICEFKN
                     TVFCTHFLMACDLLYLLYVIWQRYRVRGMDRRVCVCIWGLVILLLSYAVDMSAYYIGW
                     RRTDVIGRFGFLLFICLLAREATAVSIEKIDEGRKAEIYRELAEHDMPTGLHNRNAYD
                     EWVSKNDRDCETAIVTFDLNDLKYCNDTFGHAAGDKYIQDAAKLISEVFEPAGVCYRI
                     GGDEFCAVIRHARSEWVEECLRRLEYLERKYNEGKTDVPMRIAYGYAIFDEQYDKNME
                     ETRNRADVKMYKKKRAIKAEERK"
     gene            complement(1459..>1847)
                     /locus_tag="ACZO9R_09895"
     CDS             complement(1459..>1847)
                     /locus_tag="ACZO9R_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013361.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HIR0913408.1"
                     /translation="FSYILSLSDVKEDIAVPFINTKTTVSLSKSKKDSLTAEICRITR
                     ECLGKGENWVMTGFEDNASLFFQGDSAAVAYVEVKTFGTPSAAGTSQMTGKLCHLLSG
                     ELSIPADHIYVAYFPTDNWGWNGSNF"
BASE COUNT          467 a          368 c          562 g          450 t
ORIGIN      
        1 ttcgacagca atcagatgca gtcggagttt tcggaactga cattttttag cgtacatcag
       61 tgcgtggaag tgtatgcgga cggcgagcag atttattcgc tggaaaagag cagtacggtg
      121 ttcgggagga cgcccggaac cggatggaat acgatccagc tgcaggaggg cgtgaagagt
      181 ttcagtgtgc ggatcgaggc ggtgtacccg gcagtgcggg atcatgtgac gaggttctat
      241 caggagaatg acggacagat ggtctatcag acgatgagag gatcggttcc ggagatcatg
      301 gtcagcacag ttgacggagc gatcggtatt atgctgctca tttattatgc aattgcaaga
      361 cgcaaagtaa agatgggacc gggcattctg tattttggca tttttaccct gatgatggga
      421 atgtggtcca tgaatgaggc ggagctggtt gcctacatgg taaaaagccg gacggcggcc
      481 tcgtacaccg gctatatgat gattatgctg atgattgcgc cgtttgcagc atttttgcag
      541 gagttctccg aggtggagga gaagtatata tcaaatatta tctgcatctg ttcatttgcg
      601 aatgctatag tttgtaccgt actccacatg acggggatct gcgagtttaa aaatacggta
      661 ttttgcacac attttctgat ggcatgtgac ctgctctatc tgctctatgt gatctggcag
      721 cgttaccggg tgcgcggaat ggaccggcgg gtgtgtgtct gtatctgggg acttgtaatt
      781 ttgctattgt cttacgcggt cgacatgtcg gcgtactaca tcggatggag aagaacggat
      841 gtgatcggca ggtttggatt cctgctgttt atctgcctgc tggcgagaga ggcgactgcg
      901 gtgtcgatag agaagattga tgagggacgc aaggcggaga tctaccggga actggcggag
      961 catgatatgc caaccggact gcataaccgc aatgcttatg atgagtgggt atcgaaaaat
     1021 gacagagact gcgagactgc gattgtaacg ttcgatctga atgatctgaa gtactgcaat
     1081 gacacgttcg gacatgcggc gggcgataag tatattcagg atgcggcaaa gctgatctcg
     1141 gaagtgtttg aacctgccgg ggtgtgctac cggatcggcg gggatgaatt ctgcgccgtg
     1201 atccgccatg ccagaagtga gtgggtcgag gaatgtctgc gcagactgga atatctcgag
     1261 cggaaatata atgaagggaa aacggacgtc ccgatgcgga tagcatacgg ctacgcgatc
     1321 tttgacgagc agtatgataa aaatatggaa gagacaagga accgtgcgga tgtgaagatg
     1381 tacaagaaaa agagagcgat caaggcggaa gagagaaaat gaaaaaatgc gggaattccc
     1441 gcattttttc tgttgtagtc agaagttgct tccgttccag ccccagttgt cagtcgggaa
     1501 gtatgccacg tagatgtggt cggcaggaat ggaaagttcg ccggagagca gatggcagag
     1561 ctttccggtc atctggctgg tgcctgcagc ggacggcgtg ccgaaggttt tgacctccac
     1621 ataggcaacg gcggcagaat ccccctgaaa gaacaggctg gcattgtcct caaaaccagt
     1681 cataacccag ttctctccct tgccgagaca ctccctggta atgcggcaga tctctgcggt
     1741 cagggaatcc tttttggatt tgctcaggga aacggtggtt ttcgtattga taaatggcac
     1801 ggcaatatcc tcctttacat ctgataaaga tagtatatac gaaaata
//
LOCUS       DBMDQJ010000522         1844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21332_length_1844_cov_1.530694, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000522 DBMDQJ010000000
VERSION     DBMDQJ010000522.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21332_length_1844_cov_1.530694"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="ACZO9R_09900"
     CDS             complement(<1..974)
                     /locus_tag="ACZO9R_09900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0913409.1"
                     /translation="MKQKIGGSYIKCRLKWIAAVTGCGIVMTAVLFLNEVPAGEIAYG
                     MLLCLGILLFAATMDGISYGRRHRELFALQQSITVSLENLPEPGEILEEDYQQLLGLL
                     AEEKMRIWNTAVSEKRDLMEYYTMWVHQIKTPIAALKLLIEEEADIPGEEEPLGADRE
                     SLQQQREKRRDEELQQLFAIEQYVNMALSYMRLGSETTDFVLRQTDLDEVIRMAVRRY
                     ARHFISKKITLHYEETGARVLTDEKWLGFVIEQLLSNALKYTHEGSITIGMEEGRLTI
                     TDTGIGIRAEDLPRVCEKGYTGYNGHANQKSTGIGLYLCSTILKKLGH"
     gene            complement(984..1655)
                     /locus_tag="ACZO9R_09905"
     CDS             complement(984..1655)
                     /locus_tag="ACZO9R_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0913410.1"
                     /translation="MYRILIVEDDEIIARSLKKHLESWDYDVVCVEDFSDVMKEFAGA
                     APQLVLMDIKLPFYNGYHWCSRIREVSKVPVIFVSSASDNMNIVMAVNMGGDDFIAKP
                     FDLDVLTAKIQALLRRTYDFAGQNTVLEHRGALLNLGDATLTCGEMRVDLTKNELKIL
                     QVLMENKEKIVSRDTLMTKLWESDSFVDENTLSVNVGRLRKKLDAAGLPDFILTKKGI
                     GYHIG"
     gene            complement(1666..>1844)
                     /locus_tag="ACZO9R_09910"
     CDS             complement(1666..>1844)
                     /locus_tag="ACZO9R_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006855357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR0913411.1"
                     /translation="SCKVSGEIRKLVKGLMIESYIEDGAQKVGDGCYGKSITDPCLGW
                     EKTEKLIYELAELW"
BASE COUNT          399 a          601 c          363 g          481 t
ORIGIN      
        1 ctgtgcccca gcttttttaa aatcgtgctg caaagataca gcccgatccc ggtcgatttc
       61 tggtttgcat gaccgttata gccggtatag cctttttcgc agactctcgg cagatcctcc
      121 gcacgtattc cgatccccgt atcggtaatg gtaaggcgtc cctcctccat tccgatcgtg
      181 atgctcccct catgcgtgta cttgagcgca ttcgacaaca gctgttcgat gacaaagccg
      241 agccattttt catcggtgag cacccgtgcc cctgtctcct cgtagtgcag tgtgatcttc
      301 ttgctgataa aatgtctcgc atagcggcgc accgccatcc ggatcacttc gtcgagatct
      361 gtctgccgca gcacaaagtc cgtggtctcc gatccgagac gcatatacga aagcgccatg
      421 ttcacatact gctcaatcgc aaacagctgc tgcagttctt catccctgcg cttctccctc
      481 tgctgctgca gactttccct gtccgctccc aaaggctcct cttcaccggg aatatccgct
      541 tcctcctcga ttaaaagctt tagcgccgca atcggcgtct tgatctgatg cacccacatg
      601 gtatagtatt ccatcagatc gcgcttttca ctgaccgccg tattccagat acgcattttc
      661 tcttccgcca aaagcccaag cagctgttga taatcctcct ccaggatttc gccgggttcc
      721 ggaagattct ccagcgatac cgttatcgac tgctgcagtg cgaacagttc cctgtgccgc
      781 ctgccgtaac ttatcccatc catcgtcgct gcaaacagca gaattcccag acataacagc
      841 atgccgtagg caatctcccc ggcaggcact tcatttaaaa ataagactgc cgtcatcacg
      901 atcccgcagc cagtcactgc ggcaatccat ttcagacggc attttatgta ggatccccct
      961 attttctgct tcatcaacat cccctacccg atatgatacc cgattccttt ctttgtcaaa
     1021 ataaaatccg gaagtcctgc cgcatccagc tttttccgca gccttcccac attgacggaa
     1081 agggtgttct catccacaaa gctatcgctt tcccagagtt ttgtcatcaa cgtatcgcgg
     1141 gacacgatct tctccttgtt ttccattaac acctgcagta tcttaagttc gtttttcgtt
     1201 aaatccaccc gcatctcccc gcaggtgagt gtcgcatcgc ccagatttaa gagcgcgcct
     1261 ctatgctcca gcaccgtatt ctgccccgca aaatcatagg tccggcgcaa aagcgcctgg
     1321 atcttcgcgg tcagcacatc cagatcgaac ggcttggcaa tgaaatcatc gccgcccata
     1381 ttgaccgcca tgacgatatt catattgtcc gatgccgacg acacgaagat taccggaacc
     1441 ttcgacacct ccctgatcct gctgcaccag tggtacccgt tgtaaaaggg aagcttgatg
     1501 tccatcagca ccagctgggg cgccgccccc gcgaactcct tcatgacgtc ggaaaaatcc
     1561 tccacgcaga ccacatcata atcccagctt tccagatgct tttttaagct tcgcgcaatg
     1621 atctcatcgt cctccacgat caatatccgg tacattccct ctcctttacc acagttccgc
     1681 cagttcgtag atcaacttct cggtcttctc ccagccgaga cacggatccg tgatcgactt
     1741 tccgtaacag ccgtcgccga ccttctgcgc accgtcctcg atataactct cgatcatcaa
     1801 tccctttacc agctttctta tctctcccga caccttacag ctgt
//
LOCUS       DBMDQJ010000523         1844 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21333_length_1844_cov_1.478485, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000523 DBMDQJ010000000
VERSION     DBMDQJ010000523.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21333_length_1844_cov_1.478485"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1737)
                     /locus_tag="ACZO9R_09915"
     CDS             complement(<1..1737)
                     /locus_tag="ACZO9R_09915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR0913412.1"
                     /translation="MKPEETVFHEITLQDKAWMDQKFAEDDRNACEYSFANNFMWRNV
                     YQVRVAELHGCLVVRFIDGEIHCFSFPVGNGDKKAAVEELLAICHEEGIQLLIAPASE
                     QDRAQMLEWFPGRFLIEGDRNDYDYIYTREKLATLAGKKLHGKRNHIARFKDSGDWSY
                     EPMTAENVEECRTMTYTWIHMREEKWNEEMNQEISVLHEAFDHMQELGLVGGVLRRDG
                     QIVAFSIGERLNSETFVVHFEKAYPDLQGAYPMINQQFVLHECEGYAYVNREEDTGDP
                     GLRKAKLSYYPDILLPKYELEESGVVYADPERDAPYIQEIWQKCFGDEEDYIQFYLKH
                     RMTRENMLVIFRDGRPVAMASFLPVQYLCNGAYVDARYVYAVATLPEYRGQKLAEQIL
                     RFAEEKYQVPLILSPAEESLIRYYENLGFKNAFQGERKNVSGSDITALEVKDTEPVAC
                     MEPVTPEEYVRIRDEKCAKEGYVHWDVDAVSYAMELAASCGGGTAAVSCEDKNTRNEQ
                     EDDRDILMYDIREKELVILETTLSDDALSQVLPQLMEETGTSAASYGRERGMIWLPET
                     MADLPVAGDGYLA"
BASE COUNT          400 a          582 c          384 g          478 t
ORIGIN      
        1 tgcgagataa ccgtcgcccg caaccggcag atctgccatc gtctccggca gccagatcat
       61 gccccgctcc cgtccgtacg atgctgcgct ggttcccgtc tcttccataa gctgcggaag
      121 cacctgtgag agagcgtcgt ccgacagcgt tgtctccagt atcacgagct ctttttcccg
      181 gatatcatac atcagaatat ccctgtcatc ttcctgctca ttccgtgtgt ttttatcctc
      241 acagctgact gccgccgtac cgccgccgca gcttgccgcg agttccatcg cataggaaac
      301 cgcatccaca tcccagtgta catacccttc cttcgcgcac ttttcatccc ggatccgtac
      361 atactcctcc ggtgtgacag gctccatgca tgcaaccggc tccgtgtcct ttacctcgag
      421 cgccgtaatg tcactgccgc tcacattttt gcgttctccc tggaatgcat tcttaaatcc
      481 cagattttca tagtaacgga tcagcgactc ctcggccggc gacagaatga gcggcacctg
      541 atatttttct tctgcaaagc gcagaatctg ttccgcaagc ttctgtcccc ggtattccgg
      601 cagtgtcgca accgcataca catagcgcgc gtccacatac gctccgttgc acaggtactg
      661 caccggcaga aaacttgcca tcgctaccgg tcttccatcc cggaaaatca cgagcatatt
      721 ctctctcgtc attcggtgct tcagataaaa ctgaatataa tcttcctcgt cgccaaagca
      781 tttctgccag atttcctgaa tatacggcgc atcccgctcg gggtccgcat agaccacccc
      841 gctctcctcc agttcgtatt ttggaaggag aatatccgga taataggaaa gctttgcctt
      901 tctaagtccg ggatcgccgg tgtcctcctc gcggttgaca tacgcatacc cctcacattc
      961 atgcaggaca aactgctggt tgatcatcgg atacgctccc tgaagatccg gatacgcctt
     1021 ttcaaaatgc acaacaaacg tctccgaatt cagccgctcc ccgattgaaa acgcgacgat
     1081 ctgcccgtct ctccggagca ctccgccgac aagtccaagc tcctgcatat ggtcgaacgc
     1141 ctcatggagt accgagatct cctggttcat ctcctcattc cacttttcct cgcgcatgtg
     1201 aatccacgta tacgtcatcg tgcggcattc ttctacattc tcagcggtca tcggctcata
     1261 agaccagtct cccgaatctt taaagcgggc aatgtgattg cgctttccgt gcagtttctt
     1321 gccggcaagc gtcgcaagct tctctcttgt atagatatag tcatagtcgt tccggtctcc
     1381 ctcgattaag aatcttcccg ggaaccactc gagcatctgc gcgcggtcct gctcgctggc
     1441 aggcgctatc aacagctgga ttccctcctc atggcagatc gcaagcagct cctccaccgc
     1501 cgcttttttg tcgccgtttc cgaccggaaa tgaaaagcag tgaatctctc catctataaa
     1561 ccgaactacc aggcagccgt gaagctccgc cactctgacc tgatagacat ttctccacat
     1621 aaaattattt gcaaacgagt attcgcaggc attccggtca tcctccgcga acttctgatc
     1681 catccatgcc ttatcctgca gtgttatctc gtgaaatact gtttcttctg gtttcataaa
     1741 tacctcaatt ctaaattttt aaaaggatcg agtaggagga atttctctta aataagaaat
     1801 tccgacctct cacaccaccg tgcgtaccgt tcggtacacg gcgg
//
LOCUS       DBMDQJ010000524         1837 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21427_length_1837_cov_2.153226, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000524 DBMDQJ010000000
VERSION     DBMDQJ010000524.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21427_length_1837_cov_2.153226"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="ACZO9R_09920"
     CDS             <1..685
                     /locus_tag="ACZO9R_09920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913413.1"
                     /translation="AAGFVASVFGNVLWMAGFCILFVINLVRSVRKAEKKDNRLVWLE
                     WLLAGYCALIVLTTRFPYFKVAYPLMFLVIVMELNFFAETTQRPHRIKMVYSILAVVF
                     TCSSLVYTYYNKNTEVRVSDQVEAALEAADTDRLILIREHAEGYECFPLLGRYESVYV
                     LTNGEEKLDALLADTLIGEQKDITILITNTKADARTFQRWVSGHGFEDDMDILCKTGK
                     YEVIKWHRE"
     gene            702..1730
                     /locus_tag="ACZO9R_09925"
     CDS             702..1730
                     /locus_tag="ACZO9R_09925"
                     /inference="COORDINATES: protein motif:HMM:NF039646.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6077 domain-containing protein"
                     /protein_id="HIR0913414.1"
                     /translation="MNSALGIVIGLVVLSCIFWILGAGVCRLMGRKEGPGFLWLFGMA
                     LFLGIFSVVDIFTEAGGAAFHVLFYTALAVLVIAVALGVVYLVRAGRKEGGSWMRYPA
                     RDVWLLLFLVLLVVQIWYGMGNQLYVSRYDTSYYNGNAINALYTDTMYQYDAYTGVYV
                     GKAVAWHDSYSMLIAFLAKLFFMHPLVVVNRIMGVIEIVAANLAVYEIALRLSKGNRA
                     VAVWTAIIRALISVLCWNLGEVPGYYLWTRMAESKSMLANVYLPLALLAIVLAAEETE
                     KKQCWIMLGVFAFAGATMSMSGVFMIPMLLGVGLLPVLLRRKKLKYWGYAVLSVLPCL
                     AAGVLSFL"
BASE COUNT          389 a          354 c          579 g          515 t
ORIGIN      
        1 tgcggcagga tttgttgcgt cggtgttcgg aaatgttctg tggatggcgg gattttgcat
       61 tttgtttgtt ataaatcttg ttcgatccgt tagaaaagcg gagaaaaaag ataaccggct
      121 ggtctggctg gaatggcttc tagcaggcta ctgtgcattg attgttctga ccacccggtt
      181 tccttatttt aaggtggcat atccgttgat gtttcttgtg attgtaatgg agttgaattt
      241 ctttgcagag acaacccaaa gacctcacag aatcaagatg gtatattcga tacttgcggt
      301 ggttttcacc tgcagcagtc tggtgtatac ttactataat aagaacacgg aagtgcgggt
      361 gagcgatcag gttgaggcgg cgcttgaggc ggcagataca gatcgactga ttctgatccg
      421 ggagcatgcg gaagggtacg agtgcttccc gttactcggt cgttatgaga gcgtttatgt
      481 gttgacgaac ggagaagaga aactggatgc attgcttgca gatacactga ttggtgagca
      541 gaaagatatt acaattctga ttacgaacac aaaggcagac gcaaggactt tccagaggtg
      601 ggtatccggg catggcttcg aggatgacat ggatattttg tgcaagaccg gaaaatatga
      661 agttataaag tggcatagag aatagaaaaa ggaaaaaacg tatgaattct gctttgggga
      721 tcgtcattgg actggtggtg ttaagttgca ttttctggat attgggggcg ggagtctgcc
      781 gtcttatggg gcggaaggaa gggccgggct ttttgtggct gttcggtatg gcacttttcc
      841 tcgggatttt ctccgttgtg gatattttca ccgaggcggg cggtgcggcg ttccatgtgc
      901 ttttctacac ggcacttgcg gtgcttgtca ttgccgtggc gcttggcgtg gtgtatctgg
      961 tcagagccgg aagaaaagag ggcggatcct ggatgcggta tcccgcgcgc gatgtgtggc
     1021 tgttgctatt tctcgtgctg ctggtggttc agatctggta tgggatggga aaccagttgt
     1081 atgtgagccg ctatgatacc agctactata acgggaatgc aatcaatgcg ctctacacgg
     1141 acaccatgta ccagtacgat gcgtataccg gcgtctatgt gggaaaagcg gtggcgtggc
     1201 acgacagtta ttccatgctg atcgcgtttt tagcgaagct gtttttcatg catccgctgg
     1261 tggtggtcaa ccggatcatg ggcgtgatcg agatcgtggc agccaatctg gcggtctatg
     1321 agattgccct gcgtctctcc aaaggaaacc gtgcggttgc ggtgtggacg gcaatcatcc
     1381 gtgcgctcat tagtgtgctg tgctggaatc tcggcgaggt tccgggctat tatctgtgga
     1441 cgcgcatggc agagtcgaaa tccatgctgg cgaatgtata tcttccgctt gcgctgcttg
     1501 ccattgtgct ggcggcggag gagacggaga aaaagcagtg ctggatcatg ctcggtgtct
     1561 ttgcgttcgc cggggcgacg atgtcgatga gcggcgtgtt catgataccg atgcttttgg
     1621 gtgtcggact cctcccagtg ctgcttcgca ggaagaagtt aaaatactgg gggtatgcgg
     1681 tgctgtcggt gctgccgtgt ctggctgccg gtgtgctgag ttttctgtag gttgagggaa
     1741 aagaatggcg gaaatagatt tcttttatca gaatttttgg aaatattgga acggagcgct
     1801 gtatctggtg ccgtacctgg gctgcctggt gtatctt
//
LOCUS       DBMDQJ010000525         1825 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21601_length_1825_cov_1.466937, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000525 DBMDQJ010000000
VERSION     DBMDQJ010000525.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21601_length_1825_cov_1.466937"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1765)
                     /locus_tag="ACZO9R_09930"
     CDS             complement(<1..1765)
                     /locus_tag="ACZO9R_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016296112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HIR0913415.1"
                     /translation="MKYFCNPVNINYRYQFNMDPREGALKVAREAADPSMIYFKGKYY
                     IFASMNLSVWVSEDLAHWESHRLPENLPLYDYAPDVRVMGEYVYFSASRRGEVCNFYR
                     TKDILNGEYEEIPGTFDFWDPNLFVDDDKRVYFYWGCSNTTPLWGVEMDPKTMLPLGE
                     RQVMIEGDAFAKGYERAGEDHCQWPLSDEEIEIRFQGFLKMNHQTEEQLPKELVPLIK
                     GMLSQKPYIEGAWMDKWDGKYYLQYACPGAQYNVYADGVYIGDSPLGPFTLAKNNPFS
                     YKPGGFLPGAGHGSTMEDTAGNLWHTSTMRISVNQQFERRVGLWQAGRDVDGELFCNQ
                     RYGDWPMEVTGEKQDPWENPKWYLLSYGKQMTASSCEEGKGPEKAADENVQTWWRAAS
                     EKPGEWLQIDLGKNYDVRAVQINFADDKIDIPVPGKIKGTQTQPRYIEEQDHVTRWIL
                     EGSVDGEHFEVLEDKSQAETDLPHDLVVMEAGKQIRYVKLNILEIPYDQKPCISGLRV
                     FGIGEGAKPEVPEFEAVREGDVDMNVSIKENGAMGYNILWGHAPEKLYHSYMVFGNTK
                     KVGALVKGQDYWVRVDAFNESG"
BASE COUNT          420 a          526 c          334 g          545 t
ORIGIN      
        1 ttccactctc attgaaagca tccactctca cccagtagtc ctgtccttta acaagcgctc
       61 ctaccttttt ggtatttccg aataccatat agctgtggta cagtttctcc ggcgcatgtc
      121 cccataggat gttgtagccc attgctccat tttccttgat ggacacgttc atatccacat
      181 caccttcgcg tactgcctcg aattctggca cttctggttt cgcaccttcc ccgataccga
      241 atacacgtaa tcctgagata catggttttt gatcatatgg aatctccaga atgttcagct
      301 tcacatagcg gatttgtttt cctgcttcca tcacgaccag atcatgcggt aaatccgttt
      361 ctgcctgtga tttatcctcc agcacctcaa aatgctcgcc atccacagag ccttccagaa
      421 tccatctcgt cacatgatcc tgctcttcga tatatcttgg ctgtgtctgt gttcctttaa
      481 ttttccctgg aaccggaatg tcaatcttat catctgcaaa gttgatctgc acggcacgca
      541 catcatagtt tttgccaagg tcaatctgta gccattctcc cggcttctca gatgctgctc
      601 tccaccatgt ctgtacattc tcatcagcag ctttttccgg accttttcct tcttcacaag
      661 aagaagctgt catttgtttt ccataactta aaagatacca ctttggattc tcccatggat
      721 cctgcttctc gcctgtcact tccatcggcc agtcaccata acgctggttg cagaataact
      781 ctccgtctac atcccttcct gcctgccaga gcccgacacg acgctcgaac tgctggttca
      841 cactgattcg catggtcgag gtatgccaca gatttcccgc tgtatcctcc atcgttgaac
      901 catgtcctgc acctgggaga aatccgcccg gcttatagga gaatggattg ttcttcgcca
      961 atgtaaatgg cccgagcggg ctgtcaccga tatagacacc gtccgcatag acattatact
     1021 gcgcccccgg acatgcatac tgtaagtaat attttccgtc ccacttatcc atccatgcac
     1081 cctcaatgta tggtttctga ctgagcatgc ctttaatcaa cggaactagt tctttcggaa
     1141 gctgctcctc tgtctgatga ttcattttca aaaatccttg aaaacggatc tctatctctt
     1201 catctgagag tggccactga cagtgatcct ctcctgcacg ttcgtaccct ttggcaaatg
     1261 catctccctc aatcatcacc tggcgttcgc cgagtggcag catggttttc ggatccatct
     1321 ccacgcccca gagcggtgtt gtattggaac atccccagta gaaatacact cttttgtcat
     1381 catccacgaa caggttcgga tcccagaaat caaaggttcc cggaatctct tcatactctc
     1441 cgtttaggat gtctttcgtg cggtagaaat tacaaacctc tccgcgtctt gatgcgctga
     1501 agtacacata ctcgcccatc acacgcacat ccggtgcgta atcatacagc ggaaggttct
     1561 ccggcagacg atgactctcc cagtgagcca gatcttctga cacccagaca cttaagttca
     1621 tcgatgcgaa aatataatac tttcctttga aataaatcat ggacgggtct gccgcctcgc
     1681 gggcaacctt caacgccccc tctcttggat ccatgttaaa ctgatagcgg taattgatat
     1741 ttaccggatt acaaaaatat ttcataatta agcctccctc ttttgtttca gttgattctt
     1801 cactttttta taaataatta ccgta
//
LOCUS       DBMDQJ010000526         1820 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21688_length_1820_cov_1.836533, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000526 DBMDQJ010000000
VERSION     DBMDQJ010000526.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21688_length_1820_cov_1.836533"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1577)
                     /gene="recD2"
                     /locus_tag="ACZO9R_09935"
     CDS             complement(<1..1577)
                     /gene="recD2"
                     /locus_tag="ACZO9R_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SF1B family DNA helicase RecD2"
                     /protein_id="HIR0913416.1"
                     /translation="MEKLAGYVEHIIYRNADNGYTVLNLVSGEEEITCVGIFSAIAEG
                     ENIEASGDYTDHPTYGKQFKVESFEEKAPEDEEAIERYLGSGAIRGIGLALAARIVRR
                     FKADTFRIIEEEPERLAEVKGISERKAMEIADQVNEKRDLRQAMIFLQQYGITMNLAV
                     KVYQQYGQEVYGIIRENPYRLADDIEGVGFRTADEIAVRVGIRMDSDFRIRSGILYVL
                     LQASTEGHTYLPEEELTRRTGQLLEVGEEQIEKQYMDLAIERKIIMKQGENQTQIYAA
                     SFYYMEANTATMLKQLNVSYEVPDLEIEERVRRIEKQTGMELDEHQMTAVKEAVRNGL
                     LIITGGPGTGKTTTINTIIKYFEMEGLDIFLAAPTGRAAKRMSETTGFEARTIHRMLE
                     LNGGVDGAAGFERNEQNPLETDVVIIDEMSMVDISLMHALLKAVAVGTRLILVGDVNQ
                     LPSVGPGSVLRDIIRSHECNVVMLTKIFRQASTSDIIVNAHKINQGEEVTLDNKSMDF
                     FFLKRYDADVIISVVLQL"
BASE COUNT          385 a          580 c          366 g          489 t
ORIGIN      
        1 agctgcagta ccacgctgat aatcacatcc gcgtcatacc gctttaggaa aaagaaatcc
       61 atgcttttat tgtccaacgt cacttcttcg ccctgattga ttttgtgcgc attcacaatg
      121 atgtcgctcg tggatgcctg gcggaaaatc ttcgtcagca tgaccacatt gcactcgtgc
      181 gagcgaatga tgtcccgcag aacacttccc ggtcccacac tcggcagctg attgacatcc
      241 ccgaccagaa tcagtctcgt tccaacggca accgccttta acagtgcatg catcaaggaa
      301 atatccacca tggacatctc atcgataatc acaacgtccg tctcaagcgg attctgctca
      361 ttccgctcaa accctgccgc accgtccaca ccgccgttca gttccagcat gcggtgtatg
      421 gttctagcct caaacccggt tgtctcactc atgcgcttcg ccgcacgtcc ggtgggcgcc
      481 gcgagaaaaa tgtcgagtcc ttccatctca aaatacttga tgatcgtatt gatcgtcgtc
      541 gtttttccgg tacccggtcc tccggtgata atcagaagtc cgttgcgcac cgcctctttg
      601 accgctgtca tctggtgctc atccagttcc attccggtct gcttctcaat tctgcgaaca
      661 cgctcctcga tttccagatc cggaacctca tagctgacat tgagctgttt taacatggtt
      721 gccgtatttg cctccatgta gtaaaaggac gccgcataga tctgcgtctg gttttcgccc
      781 tgcttcatga tgattttccg ctcgatcgcc agatccatat actgcttctc gatctgctcc
      841 tccccgacct caagaagctg tccggttctt ctggtcagtt cctcctccgg caggtaggta
      901 tgcccctctg tggatgcctg aagcagcaca tataaaattc cgctccggat ccggaaatca
      961 gaatccatgc ggattcctac ccggacagca atctcgtctg ccgtgcggaa tcccacaccc
     1021 tcaatgtcgt ccgccagccg atacggattc tcacggataa tcccgtatac ctcctgtcca
     1081 tactgctgat atactttcac tgccaggttc atcgtgatgc cgtactgctg gaggaaaatc
     1141 atcgcctgcc gcagatcacg tttttcattc acctgatccg caatctccat cgccttgcgc
     1201 tcgctgattc cctttacttc cgccagccgc tccggctcct cctcgataat ccgaaaggta
     1261 tccgccttga atctgcgcac aatccgcgct gccagcgcca gaccaattcc gcggatcgcc
     1321 ccggaaccga gatagcgttc gatggcttcc tcatcttccg gcgctttctc ctcaaaactt
     1381 tcgaccttaa actgtttgcc gtaagtagga tgatcggtat agtcgccgga tgcctcgatg
     1441 ttctccccct ctgcgatcgc cgagaagatt cccacgcagg taatctcctc ctccccgctc
     1501 accagattga gaaccgtata tccgttgtct gcatttctgt atataatatg ttccacatat
     1561 cctgctaact tttccaaatc attcctcccg ccgaaaaaag cagagaaccc gtcacctcta
     1621 acggtgaccg ccctctgcct cgttcattct taactgccgg catctattct atgccatagt
     1681 tccgtttcat ctgctcataa agctgctgcg tataattttc tcccacctga tccacaatct
     1741 catctgccat cagttcgatc gtgctgtcca tgtgaaaatc tgtcagcgta ctgagagaag
     1801 aatccttatc ctcgtcatca
//
LOCUS       DBMDQJ010000527         1818 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21721_length_1818_cov_1.497962, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000527 DBMDQJ010000000
VERSION     DBMDQJ010000527.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21721_length_1818_cov_1.497962"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="ACZO9R_09940"
     CDS             complement(<1..388)
                     /locus_tag="ACZO9R_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AB"
                     /protein_id="HIR0913417.1"
                     /translation="MLIEHIFLGFCGLAAGLAVSAGTFAFLIVIGVIPRMIGKCNRAA
                     ETMHFENAVILGGICGNLASVFLQIRIPFGPALLCVYGISAGIFVGSIAVALAEILNT
                     FPITFRRMGLKVGLFWVMLAMAAGKVA"
     assembly_gap    455..464
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<465..1098)
                     /locus_tag="ACZO9R_09945"
     CDS             complement(<465..1098)
                     /locus_tag="ACZO9R_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AA"
                     /protein_id="HIR0913418.1"
                     /translation="MSAKSETLYLKIDQNTIVTDRHVTLGDIAKMECPDASIVRRLKQ
                     KKIYTFAEAMNPRKQKNQMKVFSILKIVELIHEEYPNVDISNEGEKDFIIEYIPNPEK
                     PKWINALKTVALCVVIFFGAAFTIMAFNNDVSVGDVFSKFYRQVMGKESSGVTELEIC
                     YSIGLAIGITLFFNHVGSKKITPDPTPIQVEMRKYEKDVDTTFIEEAGRDG"
     gene            complement(1095..1256)
                     /locus_tag="ACZO9R_09950"
     CDS             complement(1095..1256)
                     /locus_tag="ACZO9R_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-spanning protein"
                     /protein_id="HIR0913419.1"
                     /translation="MNCRKYRACLVGVLAIALICGALLYLKYGREGETPVDGVLVERN
                     STWEAGTAA"
     gene            1429..>1818
                     /locus_tag="ACZO9R_09955"
     CDS             1429..>1818
                     /locus_tag="ACZO9R_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HIR0913420.1"
                     /translation="MAVTKEILALAVEIGDALLRNGAEVYRVEDTVMHILEAYEIENY
                     DVYVLSNGIFASANEDREDACSMIRHIPLGTTHLGRIAALNQLSREICSHECSLIDAW
                     NRLERCKNISFGKPVVHIFFCGLGCGCF"
BASE COUNT          440 a          509 c          374 g          485 t
ORIGIN      
        1 ctgccacttt tcctgccgcc atcgcaagca tgacccagaa gagtccgacc tttaagccca
       61 tgcgccggaa tgtaatcgga aatgtattca ggatctccgc gagcgccacc gcgatgcttc
      121 ccacaaatat cccggcagaa ataccgtaca cgcacagaag cgccggtccg aacggaatcc
      181 ggatctgcaa aaaaacagac gccagattgc cgcagattcc tcccagaatc acggcattct
      241 caaaatgcat cgtctccgcc gcacggttgc attttccaat catgcgcggg atcacaccga
      301 tgacgatcag aaacgcaaat gttcccgcag agacggcaag tcccgcggca agcccgcaga
      361 agcccaaaaa tatatgctca attaacatcg atgctgtgtc cctttctctc cgcgttatct
      421 atgaatgtag tatctatgtc cttctcatat ttgcnnnnnn nnnngcccat cccttcccgc
      481 ttcctcgata aaggttgtat ccacatcctt ttcgtatttg cgcatctcca cctggatcgg
      541 tgtcggatcc ggcgtgatct tcttactgcc cacatgattg aaaaacagcg taatgccaat
      601 agcaagtccg atgctgtaac agatttcaag ttctgtcacg ccgcttgact cctttcccat
      661 cacctgccga tagaatttgg aaaagacatc tcccacggat acatcgttgt tgaacgccat
      721 aatcgtgaac gccgccccga aaaaaatgac cacgcagagc gcgacggtct ttaacgcatt
      781 gatccacttc ggtttctcgg gattgggaat gtattcaatg ataaaatcct tctctccctc
      841 attgctgatg tccacattcg gatattcctc atgaatcagt tcgacgatct ttaatatgga
      901 aaaaaccttc atctggttct tctgtttgcg cggattcatc gcctccgcaa atgtatagat
      961 tttcttctgc ttcaggcggc gcacaatgga cgcatccggg cactccattt tcgcaatgtc
     1021 cccaagcgtc acatgccggt ctgtcacgat tgtattctgg tcaatcttaa gatacagtgt
     1081 ctcactcttt gcactcatgc cgcagttccc gcttcccatg tagaattccg ttccaccagc
     1141 acgccgtcca ccggggtctc cccctctctc ccgtatttca gatataaaag tgccccgcag
     1201 atcagcgcga tggccagcac tcccacaagg catgctctgt attttctgca attcatattc
     1261 ctgtcctcct gtccgttttt acatcacttg catgatttat gcattattat gcctgtcctc
     1321 acagatatta tgtacaggac aactgacaaa agtaccagat tgcttttttt ataaaaacgt
     1381 gttacttttt catatggaca ttttattatc agaaaggcgg acatccaaat ggctgtaacc
     1441 aaggaaattc tggctctcgc cgtggaaatc ggcgatgcac tgcttcggaa tggtgccgaa
     1501 gtctaccgcg tcgaggacac ggtcatgcat attcttgagg catacgaaat tgaaaattat
     1561 gatgtttatg tgctttccaa cggaatcttt gcaagtgcaa acgaggatcg tgaagatgcc
     1621 tgcagcatga tccgccacat tccgctcggc accacgcatc tcgggcgcat cgctgcatta
     1681 aaccagcttt cccgcgagat ctgttcccac gaatgttcgc tgatcgacgc ctggaaccgt
     1741 ctggaaagat gtaaaaatat ttcgttcggt aaacctgtcg tccacatctt cttctgcgga
     1801 ctaggttgtg gctgtttt
//
LOCUS       DBMDQJ010000528         1817 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21734_length_1817_cov_1.744755, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000528 DBMDQJ010000000
VERSION     DBMDQJ010000528.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21734_length_1817_cov_1.744755"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="ACZO9R_09960"
     CDS             <1..431
                     /locus_tag="ACZO9R_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR0913421.1"
                     /translation="FQSANQAFFAGGVVFELLGSCLVIPLAEELLYRSYLMVTIARRY
                     SMTVAVLISAVIGALPYAAGAVEAPLAFLNGMLFGIFAALYLIRRGNIWGIGAFHAAW
                     SFVRDIVFGTPDQVATAVVLLVGMILVGGKRPWGKAESCC"
     gene            404..1675
                     /locus_tag="ACZO9R_09965"
     CDS             404..1675
                     /locus_tag="ACZO9R_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0913422.1"
                     /translation="MGESGILLLIFLLLLGAILAVSIVLIRKAERERDEALQHVADAR
                     TDFLSRISNDIKTPMNVIMGMTAVGLEESDHPEKMVECLGKIDTASRFLMGLLNDLVD
                     VSMIELGRFRLHPKPYALEDFMEAIRDMTEPECAKKGITFHMSGGENSLNVMVDPIRL
                     EQLFFNLLGNAVKFTPEGGEISFRVCNYAVHSGTFSADYVVADTGIGMSREFQELLFE
                     PFTRERRDEAERRHGSGLGLAITQNIVQQLGGSIAVTSAIGEGTKVKVHLDLPLVDIQ
                     PQKEVARGGGSRQAFAALGGKRVLLAEDHPLNVEITRKLLERRGVEVVCAEDGRQALE
                     LFMEREEHYFDAVLMDIVMPKMDGFEAARQIRRVPHKDAQMIPIIAMSAKDAREDMDA
                     CKEAGMNDYLAKPVEPQRLYQVLAEYLENPM"
BASE COUNT          411 a          360 c          613 g          433 t
ORIGIN      
        1 gctttcaaag cgcgaatcag gcgttttttg ccggcggggt tgtgtttgaa ctgctcggct
       61 cctgcctcgt gattccgctg gcggaggagc ttttgtaccg cagttacctg atggtaacga
      121 tcgcgcggcg ctattcgatg acggtggcgg tgcttatcag tgcggtgatc ggtgcgcttc
      181 cgtacgcggc gggagccgtg gaggcgccgc ttgcattttt aaacggtatg ctgttcggta
      241 ttttcgcggc gctttatctg atccggcgcg gaaatatatg gggaatcgga gcgtttcacg
      301 cggcgtggag ttttgtgcgt gacattgttt ttgggacacc ggatcaggtg gcgacggcgg
      361 tagtgctgct tgtcggaatg atacttgttg gaggaaaaag accatggggg aaagcggaat
      421 cctgctgctg attttcctgc tgcttctcgg agcgatactg gcggtcagca tcgttttgat
      481 ccggaaggcg gagcgtgaga gggatgaggc gttgcagcat gtcgcggatg caaggacgga
      541 ttttctctcc cgcatcagca atgacatcaa gacgccgatg aacgtgatca tgggaatgac
      601 ggcggtcgga cttgaagagt cggatcaccc ggagaagatg gtggaatgtc ttggaaaaat
      661 tgatacggcg agccggtttc tgatggggct tttgaatgat ctcgtggatg tttccatgat
      721 tgaactgggg agattccggc tgcatccgaa gccgtatgcg ctcgaggatt ttatggaggc
      781 gatccgggat atgacagagc cggagtgtgc caaaaagggg atcacatttc acatgtccgg
      841 cggcgagaac agtctgaatg tcatggtgga tccgatccgg ctggagcagc tcttttttaa
      901 tctgctgggg aacgcggtca agttcactcc ggaaggggga gagatctctt ttcgggtatg
      961 caattatgcg gttcacagcg gtactttttc tgcggattat gttgtcgcgg acaccggaat
     1021 cggcatgagc agggagtttc aggaactgct gtttgagccg tttacgcggg aaagacggga
     1081 tgaggcggaa cggcggcacg gcagcggact ggggctggca attacacaga atattgtgca
     1141 gcagcttggc ggaagtattg cagtcacgag tgcgatcggc gagggcacga aggtgaaggt
     1201 gcatctggat cttccgctcg tggatattca gccgcagaag gaagtggcga gaggcggggg
     1261 ttccagacag gcgtttgccg cgctcggggg gaagcgggtg cttctcgcgg aggatcatcc
     1321 gctcaatgtt gagattacgc ggaagctttt ggagcgccgg ggcgtggaag tcgtctgtgc
     1381 ggaagatgga cggcaggcgc tggagctttt tatggagagg gaagagcatt atttcgatgc
     1441 ggtcctgatg gatattgtga tgccgaagat ggacggcttt gaggcggcaa ggcagatcag
     1501 acgggtgccg cacaaggatg cgcagatgat tccgattatc gcaatgagcg cgaaggatgc
     1561 gcgcgaggat atggatgcct gtaaggaagc cggaatgaac gactatcttg caaagccggt
     1621 ggagccgcag agactgtacc aggtgcttgc ggaatatctt gagaatccga tgtgagtttt
     1681 tggacagatt gccgaaacgg tcagatgtaa aataaaaata aaataaatga cgtgtattga
     1741 cttttaaaaa gaagttgata tattagtgta ttagtactaa ggatatacta aaaataagcg
     1801 aaaggaataa taatccc
//
LOCUS       DBMDQJ010000529         1817 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21736_length_1817_cov_1.673077, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000529 DBMDQJ010000000
VERSION     DBMDQJ010000529.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21736_length_1817_cov_1.673077"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(124..360)
                     /locus_tag="ACZO9R_09970"
     CDS             complement(124..360)
                     /locus_tag="ACZO9R_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polya polymerase"
                     /protein_id="HIR0913423.1"
                     /translation="MKIKNITDIDGFFKVIDSCQGKVELITGEGDRLNLKSKLCQYVS
                     LTSILKTDVEIPELELVAYEPEDTEKIIKFLMKG"
     gene            complement(430..651)
                     /locus_tag="ACZO9R_09975"
     CDS             complement(430..651)
                     /locus_tag="ACZO9R_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913424.1"
                     /translation="MIEKVHEVNDAASNMAAITEEQSASAEKIEATAVSIQELADVVS
                     ENSVDVQKDSKEMENTAETLKEHISRFTI"
     gene            complement(676..>799)
                     /locus_tag="ACZO9R_09980"
     CDS             complement(676..>799)
                     /locus_tag="ACZO9R_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR0913425.1"
                     /translation="VKYDNLGIDYVLKDTPQLTKAEAKSAESIILEAMRQARNV"
     assembly_gap    800..809
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<810..1442)
                     /gene="pflA"
                     /locus_tag="ACZO9R_09985"
     CDS             complement(<810..1442)
                     /gene="pflA"
                     /locus_tag="ACZO9R_09985"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HIR0913426.1"
                     /translation="MGKIHSMESFGTVDGPGVRFVVFFEGCPMRCLYCHNPDTWHLED
                     GQEMSAEEILSRMERNRSFYRTGGITATGGEPMLQIDFLTELFAGAKERGIHTCLDTS
                     GIMFPGCGGTSEEERERLKKVERLLSVTDLVMLDIKHMDAEEHRKLTGQDNEKILAFA
                     RYLDEKQIPVWIRHVVVPGITFQREELEALGRFLSTLSNVEKLEVLPYHSM"
     gene            complement(1587..>1817)
                     /locus_tag="ACZO9R_09990"
     CDS             complement(1587..>1817)
                     /locus_tag="ACZO9R_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009462180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HIR0913427.1"
                     /translation="TGEEQNLVALLDAYAEKGGHHLNVNVLNRETLLDAQKHPEQYPQ
                     LTIRVSGYAVNFIKLTKEQQDEVIARTFHERM"
BASE COUNT          388 a          554 c          375 g          490 t
ORIGIN      
        1 ccttgctgtt cggctataca cttcctcgtt gcctaggcgt gttcgggact ttcacccgtt
       61 agagcgcgcc catggcgcgc aaaccaaaaa agaaccgcca acctgtgttg acggttcttt
      121 ctctcagccc ttcatcagga acttaataat tttctctgta tcttccggct cgtaagcgac
      181 aagctcaagc tccggaatct ccacatccgt ctttaaaata ctggtaagtg acacatactg
      241 acaaagcttt gacttgaggt tcagtctgtc cccctctccg gtaatgagtt ccacttttcc
      301 ctggcaggag tctattacct taaaaaatcc gtcgatatcc gtgatgttct tgatcttcat
      361 aatacatgct cctttcttaa tctgagtctc tatctacact ataatacgaa agtacctctt
      421 ctgcaagttc taaatcgtaa acctggaaat atgttccttc agcgtctcag ccgtgttttc
      481 catctccttc gagtctttct gcacgtccac actgttctcg gagacaacat ccgccagttc
      541 ctgaatgctg accgcagtcg cctcaatctt ctccgcggat gccgactgtt cttccgtgat
      601 agccgccata ttggacgcgg catcgtttac ttcatgcact ttctctatca ttccctgcac
      661 gatccgttcc ggcagtcaga cgtttctcgc ctgacgcatt gcctccagaa tgatgctctc
      721 cgcactcttc gcctccgcct tggtgagctg tggcgtatct tttaatacat aatcgatgcc
      781 aaggttatca tatttcactn nnnnnnnnnc atggagtgat acggaagcac ctccagcttt
      841 tccacattcg agagtgtcga aaggaaccgg cccagagcct ccagttcttc cctctgaaat
      901 gtgatccccg gaacaaccac atgccggatc cagaccggta tctgtttttc atccagatac
      961 cttgcaaacg caagaatctt ctcattgtcc tgtccagtca gcttccggtg ctcctccgca
     1021 tccatatgtt tgatgtcaag catcacaaga tccgtcactg agagcagccg ttccaccttc
     1081 tttaagcgtt cccgctcctc ctccgacgtg ccgccacacc cggggaacat aatgcccgac
     1141 gtgtccagac aggtatgaat cccgcgttcc tttgcccctg caaagagctc cgtcaaaaaa
     1201 tcaatctgga gcatcggctc tccgccggtc gcagtgattc cgccggtccg gtaaaacgac
     1261 cggttgcgct ccatgcgtga cagaatctct tccgcgctca tctcctgccc atcctccaga
     1321 tgccaggtgt cgggattatg gcagtacagg cagcgcatgg gacatccctc aaagaacacc
     1381 acaaagcgga ctcccggtcc atccaccgtt ccaaagcttt ccatcgaatg aatttttccc
     1441 ataaagtcct cctttttgat cccatccatc tgataatcat aatacaatcg cgcgagccgg
     1501 cgtatcccgg tgtccctgcc gcaaaagcag cagggcaccg gttccggttc gcgcgattta
     1561 tcctgtctgt atcacttcca catgaactac atgcgctcgt ggaaggttct tgcaatgact
     1621 tcgtcctgct gctctttggt cagcttgata aagtttaccg catatccgga aacacggatt
     1681 gtgagctgcg gatactgctc cgggtgtttc tgtgcatcca aaagcgtctc gcggttcaat
     1741 acgttgacgt tcaagtgatg tccgcccttc tcggcgtaag catccagcag cgccacaagg
     1801 ttctgctcct cgcccgt
//
LOCUS       DBMDQJ010000530         1812 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21810_length_1812_cov_1.599065, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000530 DBMDQJ010000000
VERSION     DBMDQJ010000530.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21810_length_1812_cov_1.599065"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            162..1811
                     /locus_tag="ACZO9R_09995"
     CDS             162..1811
                     /locus_tag="ACZO9R_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HIR0913428.1"
                     /translation="MRNIFKIFFADVKRISTNVVAVVVILGLCILPSLYAWFNILSNW
                     DPYGPSATRNLKIAVASDDEGVTILGNSVNVGSSVISALESNTTMGWVFTDTTDEAID
                     GVYDGSYYAALIIPENFTEDMISFLGGEVDHPDIIYYENAKKNAIASKITQKAKTAVQ
                     EQVNSTVVSTLAETTMKVTNALSDVDAEDSDPAESVLVRLNNLSSDLQSYINILNSFV
                     SIMNSAQSIVEASQTMLPNVNNMVSNGQASINSMESLLVAGNSTVSSTADMVSYSFDM
                     MNDSLNNVSSVITNALNRLSEYSQGTSVTVQGLSAMMPYVRQLFDTSVSGWEDTAPED
                     MQAQINAIRTQLDTITADLTALENNADTSMETVRNLSNKIVTEIANCQKAVDGLRNDF
                     AYNVKPKLENTMSSMQQSMIAAAGILDGVDADFNGVENALSDYQSTLERGSNSLGGSL
                     SMAQELLAKLNSVTADLVSLQQNDQYQELLEIVKSNPEELGAFISSPVNLETVGIYEI
                     ENYGSAMSPFYTILALWVGALILVAIIHVKVHPVEFCECRK"
BASE COUNT          488 a          386 c          520 g          418 t
ORIGIN      
        1 aacgcagact gtggaaaagg cacagactgc ggaagagaca gagaaacaaa aggatgcgga
       61 tgcacaggct gtagaagatg cagatgcaca gaaagatgcg gatgcacagg ctgtggaaga
      121 tgcagatgca cagaaagatg cggacacgga ggtgaaggag aatgcgtaat atatttaaga
      181 tttttttcgc cgatgtcaag cgcatcagca ccaacgtggt ggctgtcgtt gtgatcctgg
      241 gactctgtat tctgccgtct ttgtatgcat ggtttaatat tttgtcaaac tgggatccgt
      301 acggaccgtc cgcaaccaga aatcttaaga tcgcggtggc gtcggacgac gagggagtca
      361 caattttagg gaacagtgta aatgttggaa gcagtgtcat ctctgcgctt gagtcgaaca
      421 cgacgatggg ctgggtgttt acggatacga ccgatgaggc gatcgacggc gtctatgatg
      481 gaagttatta tgcggcgctc atcatcccgg agaattttac ggaagatatg atcagtttcc
      541 tcggcggaga ggtggatcat ccggatatta tctattatga gaatgccaag aaaaacgcca
      601 ttgcctccaa gatcacacag aaggccaaga cggctgtgca ggagcaggta aacagcacgg
      661 tggtgagtac ccttgcagag accacgatga aggtgacgaa cgccctctca gatgtggatg
      721 cggaagattc cgatccggcg gagagtgttc tggtaagact caataattta agcagcgacc
      781 tacagagtta catcaatata ttgaactcgt ttgtcagcat tatgaattcc gcccagagta
      841 ttgtggaggc gagtcagacc atgcttccga atgtgaacaa catggtttcc aacggtcagg
      901 catccatcaa cagcatggag agtctgctgg ttgcgggcaa cagcacggtg agttcgacgg
      961 cggatatggt gtcgtacagc tttgacatga tgaacgattc gttaaataat gtgtcgtccg
     1021 tcattaccaa tgcgttaaac cgtctctcgg agtattccca gggcacttct gtcacggtac
     1081 agggcttatc ggcgatgatg ccatacgtga ggcagctgtt tgatacttcg gtatccgggt
     1141 gggaggatac ggcgccggag gatatgcagg cgcagattaa tgcaatccgc acgcagttgg
     1201 ataccatcac tgcggacctg acagcgctgg agaacaatgc cgatacatcg atggagacgg
     1261 taaggaatct cagtaataaa attgtgacgg agattgcaaa ctgccagaaa gcggtcgatg
     1321 gactgcgcaa tgattttgct tacaatgtca agccgaagtt agagaacaca atgagctcca
     1381 tgcagcaatc catgattgcc gcagcgggta ttttagacgg cgtagatgcc gattttaacg
     1441 gtgtggagaa tgcactttcc gattatcaaa gcacgcttga gcgtggaagc aacagccttg
     1501 gcggatcact gtccatggcg caggagcttc tcgccaagct aaacagcgtc acggcggatc
     1561 tggtaagctt acagcagaat gaccagtatc aggagctgct tgaaatcgta aagagtaatc
     1621 cggaggagct gggagccttt atttcttccc cggtaaatct ggagacggtc ggtatctatg
     1681 agattgaaaa ctacggctcg gcgatgtctc cgttctacac gatcctcgct ctctgggtag
     1741 gagcgctgat tctggttgcg atcatccacg tgaaggtgca tccggttgaa ttctgtgaat
     1801 gtcgaaaatg aa
//
LOCUS       DBMDQJ010000531         1812 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21813_length_1812_cov_1.313267, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000531 DBMDQJ010000000
VERSION     DBMDQJ010000531.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21813_length_1812_cov_1.313267"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="ACZO9R_10000"
     CDS             complement(<1..245)
                     /locus_tag="ACZO9R_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913429.1"
                     /translation="MKKYQIMYYVGAAISLLVGLWHFTVPWMFGWASYIPYETLVVPI
                     NYVNLCFSFLLSGLSLTVILWGKKVFVQNPEVFYLYG"
     gene            complement(252..1001)
                     /locus_tag="ACZO9R_10005"
     CDS             complement(252..1001)
                     /locus_tag="ACZO9R_10005"
                     /inference="COORDINATES: protein motif:HMM:NF014566.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HIR0913430.1"
                     /translation="MKRKDRIILKTAMATLLLLLFFFAQGAIVVITGIEGIPSALIRG
                     AVIWGLVIITLANSIIRYKGISKLGFRSAEKGAAKRMLYFSPLLLIALSPFTAGININ
                     GGAALILANLFLTLGIGMAEEIFFRGIIFGLWLEHGVGKAMIISSVLFGFSHLLNIAG
                     GAELGETVLQICFALVYGMVFALIFAESGSLLPCVLLHALHDFCSFISGEASAQFEIF
                     LGAVQFIVLLVYFLYLFRITARKREPALKGR"
     gene            complement(1063..>1812)
                     /locus_tag="ACZO9R_10010"
     CDS             complement(1063..>1812)
                     /locus_tag="ACZO9R_10010"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0913431.1"
                     /translation="MKRIKVKQINNHIWLLNDNDESTGYLVTGSERGLIIDTMTGYGN
                     VRKVAEEITDFPLTVVNTHGHPDHIYGNVYFEKVYIHPADIPVAERFYQDPAFVAAIR
                     QMNRQSAEFLTLTEGDVFDLGGLFLEVYELPGHTPGGIVLLDRKDRILFSGDSILEQT
                     WMQLPECLPMEQFLASLDKIQKMRGEFDYLLTGHNLELVNASFCEVHRKAVWEVCVGI
                     KDKDEPYSYFGGTCMAHPYGKEPRRIVYRER"
BASE COUNT          495 a          491 c          367 g          459 t
ORIGIN      
        1 aagccgtaga gatagaacac ctccggattc tgcacgaata cctttttgcc ccacagaatg
       61 acggtaaggc tcagccccga cagtaaaaat gaaaagcata ggttgacata attgatggga
      121 accaccagcg tttcatacgg tatatacgaa gcccagccaa acatccatgg tacggtaaaa
      181 tgccacagtc ccacaagcag gcttatcgcc gcgcccacat agtacatgat ttgatatttt
      241 ttcatcggtt ttcacctgcc tttcaaagcc ggttcccttt tgcgagcagt tattcggaac
      301 agatagagga aatagaccag caagacgata aactgtaccg cgcccagaaa tatctcaaac
      361 tgcgcggaag cctccccgct gatgaaggaa cagaagtcat gcagagcgtg gagcagtacg
      421 caggggagaa gactgccgct ttcagcgaaa atcagggcaa ataccatccc gtagaccagg
      481 gcgaagcaga tttggagaac tgtttccccc aattccgcgc ctccagctat gttcaggaga
      541 tggctgaacc cgaatagaac ggatgagatt atcattgcct ttcccacgcc gtgctccaac
      601 cacaggccga agataatgcc tcggaaaaat atctcctccg ccatcccgat tccaagcgtc
      661 aaaaacaagt ttgctaaaat caatgccgca ccaccgttaa tgtttattcc cgccgtaaaa
      721 ggggaaaggg caatgagcaa aagcggcgaa aaatagagca ttctctttgc cgcccctttt
      781 tccgcactgc gaaatcccag ctttgagatt cctttgtatc ggatgatgga attcgccaat
      841 gtgataatta caagccccca gatcacagca cccctgatca gcgcggaggg aatgccttct
      901 attcccgtga tcaccacgat tgctccctgc gcgaaaaaga acagcagcag caaaagcgtt
      961 gccatagcgg ttttcagtat gattctgtct ttccgtttca ttttaagcct cctgtcccag
     1021 tcacagacat atgtcttacc aagcgatcgg gtctctttcg tcctatcttt cccgatatac
     1081 aattcttctt ggttcttttc cataaggatg agccatgcag gttccgccaa aatagctgta
     1141 gggctcatcc ttatccttga ttcccacgca gacttcccag actgcctttc tatgtacttc
     1201 gcaaaaagaa gcattcacaa gctccaggtt atgtcctgtc agaagatagt caaattctcc
     1261 ccgcattttc tgtattttgt ccagagatgc cagaaactgt tccatgggaa gacactccgg
     1321 cagctgcatc catgtctgtt ccagaatgct gtcccccgaa aataaaattc tgtctttcct
     1381 gtccaaaagc acaatcccgc ccggtgtgtg tcccggcagt tcatacactt ccagaaaaag
     1441 acctcccaga tcaaatacat cgccttccgt taacgtcaga aactcagccg actgccggtt
     1501 catttgcctg attgctgcca caaaagcagg atcttgataa aacctttcgg ccaccggaat
     1561 atcagcagga tgtatatata ccttctcaaa gtatacattt ccatagatat gatcaggatg
     1621 accgtgggta ttgacaactg taagaggaaa atccgtgatc tcttcggcca ccttccgcac
     1681 attcccatat cctgtcatag tgtcgataat cagtcctctc tcacttcccg tcaccagata
     1741 tcctgtggac tcatcattgt catttaacag ccagatatga ttgttgatct gttttacttt
     1801 aattcttttc at
//
LOCUS       DBMDQJ010000532         1811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21828_length_1811_cov_1.408187, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000532 DBMDQJ010000000
VERSION     DBMDQJ010000532.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21828_length_1811_cov_1.408187"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(240..818)
                     /locus_tag="ACZO9R_10015"
     CDS             complement(240..818)
                     /locus_tag="ACZO9R_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009268 - response to pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HIR0913432.1"
                     /translation="MKETLKRIKADIIVSALLCIALGIVLLVWPAETIDVFCKILAVG
                     LIIMGGVDIASYFMNRSIHPFAGALGLIVLLIGIWIFLRPESIVSLIPIVIGVILCVH
                     GIQDLKLAIETKRNGYEKWWSMLIIAAVSLVFGVLCIVNAFGMVKLALQFIGIALIYD
                     GISDLWVANRAVHAAKMMKQEAEAVDVDYKEI"
     gene            1059..1541
                     /gene="ybaK"
                     /locus_tag="ACZO9R_10020"
     CDS             1059..1541
                     /gene="ybaK"
                     /locus_tag="ACZO9R_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR0913433.1"
                     /translation="MGKEVKTNAMRILDKNKIPYEILNYECGEFIDGLHTAEATGAPV
                     EQSFKTLVMQGKSRQYYVFVIPIADEVDLKAAARAVGEKSVEMIHVKDITAITGYVRG
                     GCSPLGMKKQFPTIVHESAAAFDKIYISGGRIGTSITVNPDSLLTVVRGQYADVIRHE
                     "
     gene            complement(1577..>1811)
                     /locus_tag="ACZO9R_10025"
     CDS             complement(1577..>1811)
                     /locus_tag="ACZO9R_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913434.1"
                     /translation="VVASYDTFDEAADAYNQDAGKIDALISDFSATSEELLASIDGVL
                     DAMEGMATAANEGAKGTIDIAQKTVEVKSKADT"
BASE COUNT          483 a          488 c          383 g          457 t
ORIGIN      
        1 agccaaaata gccggtttaa ataagttttt gcaaatctat tatgcaagag tgatggaaat
       61 atataagaat atagcatagt tgcataagaa aaaagcctga ataatcaggc ctatttctta
      121 tgcaaaaaaa attaaaaatt gtgttgacat ctcttaacaa atctaaaaag tccgcccatt
      181 gtctccggcg tgccggacat gcacccgttg gattgtccaa acgtggaaga gacgctatct
      241 tatatctctt tatagtctac atccacggct tccgcttcct gcttcatcat ctttgccgca
      301 tgcacggcgc ggtttgccac ccacagatcc gagataccgt catagatcag ggcaattccg
      361 ataaactgca gtgccagctt taccatgccg aacgcattca cgatacagag cacaccgaac
      421 accaggctga ccgctgcaat gattaacatg ctccaccatt tctcataacc gttgcgcttc
      481 gtttctatcg caagcttcag atcctggatt ccatgtacgc agaggatcac gccgatcaca
      541 atcgggatca ggctcacaat gctctccggg cgcaggaaaa tccagattcc gattaacaat
      601 acaatcagtc ccaatgctcc cgcaaacgga tgaatactgc ggttcataaa ataggatgcg
      661 atgtcgacgc ctcccataat gatcaatcca acggcaagga ttttacagaa tacatcgatc
      721 gtctcagccg gccataccag aagcacaatg ccaagtgcaa tgcaaagcag cgccgagact
      781 atgatgtctg ccttgatccg ctttaatgtt tctttcatat cacatacctc tctgtcactt
      841 tttctgcatt atactctatt caatccacag tttcaagaaa caatgcggcg gctttgtcta
      901 ttcccattca gcccggtctg tcccgcttga ctttttttca caattccact agactatggg
      961 cacaatgaat catatgtccc gccgcagtgc aatccacgcg gagcgttttt ccttcacagg
     1021 acatattttc cggaaagaaa caaaggagtg aatattttat gggaaaagaa gtaaagacga
     1081 atgctatgcg cattcttgat aaaaataaga ttccgtacga aattttaaat tatgagtgtg
     1141 gagaatttat cgacggactg cacaccgccg aggcgaccgg cgccccggtg gaacagtcct
     1201 tcaagactct ggtgatgcag ggaaaaagca ggcaatacta tgtattcgtt attcccatcg
     1261 cggacgaggt ggacttaaaa gccgccgcca gagccgtcgg cgaaaaatcc gtagagatga
     1321 ttcacgtcaa agacatcact gccatcacgg gttatgtccg cggcggctgc tctcctctgg
     1381 gcatgaaaaa gcagttcccg accatcgtgc acgagagcgc tgcggctttt gacaagatct
     1441 acatcagcgg cggacgcatc ggcacctcca tcacggtcaa tccggattcc ctgcttaccg
     1501 tggtgcgcgg ccagtatgcc gacgtcatcc ggcatgagta agttcttctt ccatctcctc
     1561 taagatgtgg agcagatcac gtatctgcct tggatttcac ttctacggtc ttctgtgcaa
     1621 tatcgatcgt tcccttggca ccttcgttcg ctgccgttgc cattccctcc atcgcatcca
     1681 gcacgccatc aatcgatgcc agaagttcct cggatgtcgc gctgaaatcg ctgatcagtg
     1741 catcgatctt tccggcatcc tggttataag cgtctgccgc ctcatcaaac gtatcatagc
     1801 tcgccaccac a
//
LOCUS       DBMDQJ010000533         1811 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21832_length_1811_cov_1.356725, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000533 DBMDQJ010000000
VERSION     DBMDQJ010000533.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21832_length_1811_cov_1.356725"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="ACZO9R_10030"
     CDS             complement(<1..1772)
                     /locus_tag="ACZO9R_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR0913435.1"
                     /translation="MSLAACTTTKEIAYFQDTERDEMMKIVEDNLPRIKPDDILSITV
                     SSSKPELAAPYNLLRATQDVNSSSNRWEQEAYQVNSEGYINFPVLGALKVAGMTREEL
                     VVMLQKRLADLGKILGTVSVFLCVLLFGMAILQKRDVGEMLITAISLAVAAVPEGLPA
                     IVTMVLALSVSRMVKANAIVKRLPSVETLGCVSVVCSDKTGTLTQNRMTVKKCYVNGR
                     VHAVKELHPADDRYFLQGFSLCNDASLHGGERRGDPTELALLDMAAAFGIQRETLEDT
                     MPRIDEIAFDSERKRMTTLHETLDGRISFTKGSPDEILERCRYVWSNGKTAAFSAAAR
                     EQAKRGLAEMTEAGLRVLAIGMHPKAAQMEESGLTFLGMAGMEDPVRPEAAEAVEQFA
                     RAGVRTIMITGDRADTALAIAHQLGIAERRDECVTGEEMEQMDEQTLAARMGRTRVFA
                     HVSPEHKVRIVSACKKNGEIVAMTGDGVNDAPSLKSADVGIAMGMAGTDVARNAADMV
                     LADDNFATIAGAIAQGRSIYENIRKSVLFLLSSNFGEIITMLAAVAMGLTSPLKSSHI
                     LWINLITDSLPALALGVDVEEQKD"
BASE COUNT          382 a          585 c          423 g          421 t
ORIGIN      
        1 taatccttct gttcctccac atccactccg agtgccagcg ccggcaggga atccgtaatc
       61 agattgatcc agagaatatg gctcgacttt aagggcgatg tcagccccat ggcaactgcc
      121 gccagcattg tgataatttc tccgaaatta gatgaaagta aaaacagcac cgacttgcgg
      181 atattttcat agatgctgcg cccctgcgca atcgctcctg caatcgttgc aaaattgtca
      241 tccgccagca ccatatccgc cgcatttctg gcgacgtccg ttcccgccat tcccatggca
      301 attcccacgt ccgcagattt taacgacgga gcgtcattga ctccgtcccc tgtcatagcg
      361 acaatctccc cgttcttttt gcatgcggac acgattctga ccttgtgctc cggggataca
      421 tgggcaaaca ccctggttcg tcccatccgc gccgccagcg tctgttcgtc catctgctcc
      481 atctcctcgc ccgtcacaca ttcatctctc cgctctgcaa tgccgagctg gtgtgcaatg
      541 gcaagcgccg tgtccgccct gtccccggta atcatgatcg tccggactcc ggcacgtgca
      601 aactgttcca ccgcctccgc cgcctcgggg cgtaccgggt cttccattcc tgccatcccc
      661 agaaacgtaa gcccgctctc ctccatctgt gccgctttag gatgcatgcc aatcgccagc
      721 acgcgaagtc ccgcttccgt catctcggca agtccccgct ttgcctgttc tctcgccgcc
      781 gcagaaaacg ccgccgtctt gccgttgctc cacacatacc ggcaccgctc caggatttca
      841 tccggcgatc cctttgtgaa agagattctg ccgtccaacg tctcatgcag cgtcgtcatc
      901 cgtttccgct ccgaatcgaa ggcaatctcg tcgatccgcg gcatggtatc ctccaatgtc
      961 tcgcgctgta tgccgaacgc tgccgccata tccaagagcg ctaattctgt cggatcgccg
     1021 cggcgttccc ctccgtggag ggatgcgtcg ttgcagagag aaaatccctg caaaaaatac
     1081 cggtcgtcgg ccggatgcag ttcttttacc gcatgcactc taccgttcac gtaacatttt
     1141 tttacagtca tgcggttctg tgtcagtgtt cccgtcttat ccgagcacac aacgctgaca
     1201 catcccaatg tctccacgct cggcaggcgc tttacaatgg catttgcctt caccatccgc
     1261 gagacggaaa gtgccagcac catcgtcaca atggcgggaa gtccctccgg caccgccgcc
     1321 accgcaagag agatcgccgt aatcagcatc tctcccacat cccgtttttg caggattgcc
     1381 atgccaaaaa ggagcacaca taagaatacg gacaccgtgc ccaaaatttt gccgaggtcc
     1441 gccagccgct tctgcaacat cacgacgagc tcttcccgcg tcatgccggc caccttgaga
     1501 gcacccagta caggaaaatt gatatacccc tcgctgttga cctgataggc ttcctgctcc
     1561 cagcgattgg aggacgaatt gacatcctgt gtggcgcgca gcagattgta gggagccgcc
     1621 aattcgggct tggaagaaga gaccgtaatg gagagaatgt cgtcgggctt tatccgggga
     1681 agattgtctt cgacgatctt catcatctca tcccgttccg tatcttgaaa ataggctatt
     1741 tccttggtcg tggtacatgc agcaagcgac atggccaaca cgaaagaaag gcagatttta
     1801 ttcatgagca a
//
LOCUS       DBMDQJ010000534         1806 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21896_length_1806_cov_1.777713, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000534 DBMDQJ010000000
VERSION     DBMDQJ010000534.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21896_length_1806_cov_1.777713"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="ACZO9R_10035"
     CDS             <1..1320
                     /locus_tag="ACZO9R_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR0913436.1"
                     /translation="YSDDLYRYLRSKGYDDEILKDSGLVTIDEVRGGHDKFWNRAMFP
                     IMDVHNKVIGFGGRVMGDGEPKYLNSPETKIFDKSRNLYGLNFARATKKPQLLLCEGY
                     MDVIALHQAGFDNAVASLGTALTSGHANLLKRYTKEVYLTYDSDGAGIKAALRAIPIL
                     KEVGITTKIINMKPYKDPDEFIKALGAEAYQERIDQAENSFLFEVRMEEQQHDMHDPE
                     GKTAFYNAVAKMLCGFTEKLERDNYIEAVAAKYMISPDDLRRLVNQQGLKAGLAGGGR
                     APQSVADAQDGARTEYKKSAKKREDGMVQSQKLLLTWLTNSPALFPKVQRYVGADDFT
                     DPICHSVAKMLFEQYEKDGTVNPARIISTYEDEEQQREAAGILNATIHRVDTREEREK
                     ALRETVIRVRENSINHKLANTFDVQEMAALAKEKNELPGTVHIPLEE"
     gene            1404..>1806
                     /locus_tag="ACZO9R_10040"
     CDS             1404..>1806
                     /locus_tag="ACZO9R_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HIR0913437.1"
                     /translation="MAKKKENTDNKEVLGENKENSAAKTADAGAENGVASAQEKFQIK
                     LRELLALAKKKKNMLEYQEISDFFSDMQLDAEQFEKILDFLEANNIDVLRITDDDVDD
                     EMLLDVDDEDEIEVEKIDLSVPDGVSIEDPVR"
BASE COUNT          517 a          380 c          561 g          348 t
ORIGIN      
        1 tacagcgatg atctgtaccg gtatctgcgg tcgaagggct atgacgacga gatcttaaag
       61 gattccggtc ttgtcacgat cgatgaggtg cggggcggac atgataagtt ctggaaccgt
      121 gccatgtttc cgatcatgga tgtgcacaat aaggtgatcg gatttggcgg gcgcgtcatg
      181 ggagatggag agccaaagta tctcaattcc cccgagacga agattttcga caagagccgc
      241 aatctttacg gactcaattt cgcacgggcg acaaaaaagc cgcagctgct cttgtgcgag
      301 ggatatatgg atgtgattgc actgcaccag gcagggtttg acaatgccgt ggcgtcgctc
      361 ggtacggcgc tcacaagcgg tcatgccaat ctgctcaagc gctataccaa ggaagtctat
      421 ctgacgtatg acagcgacgg ggcggggatc aaggcggcgc tcagagccat cccgatttta
      481 aaagaagtcg gaatcacgac caagatcatc aacatgaagc cgtataagga tcccgatgag
      541 tttatcaagg cgcttggcgc ggaggcgtat caggagcgca tcgaccaggc ggagaacagt
      601 tttctgtttg aggtgcgcat ggaagagcag cagcacgaca tgcacgatcc ggagggcaag
      661 acggcctttt acaatgcggt cgcgaagatg ctttgcggat ttacggagaa gttagagcgc
      721 gataattata tcgaggcggt tgcagccaaa tacatgatct cgccggacga tttgagaagg
      781 ctggtcaacc agcagggatt aaaggctgga cttgccggag gaggcagggc gccgcagagc
      841 gtcgcggacg cacaggacgg tgcgcgcaca gaatacaaaa agagtgcgaa aaagcgcgag
      901 gacggcatgg tgcagtcgca gaagcttcta ctgacgtggc tgacgaacag tccggcgttg
      961 tttccaaagg tgcaaagata tgttggtgca gatgatttta cggacccgat ctgccacagt
     1021 gtggcaaaaa tgctgtttga acagtatgag aaggacggga cagtcaatcc tgcgcggatc
     1081 atcagcacct acgaggatga ggagcagcag cgggaggcag cgggaatctt aaacgcgaca
     1141 atccaccggg tggataccag ggaggaacgc gaaaaagcgc tgcgcgagac cgtgatccgc
     1201 gtgcgggaga acagcatcaa tcataagctg gcaaacacgt ttgacgttca ggagatggcg
     1261 gctctggcaa aagaaaagaa tgagctgccg ggaacggttc atattccgct ggaggaatga
     1321 gagcgggcaa agaagcagga atgtggatga aaatctgcat ttaaggacaa aatagtaacg
     1381 aactatgaga ggatgaagcg aaaatggcaa aaaagaaaga aaacaccgac aataaggaag
     1441 ttctgggaga aaacaaggag aactctgctg cgaaaactgc cgatgcggga gcagagaacg
     1501 gggtggcaag cgcccaggag aagttccaga taaagttaag agaactgctt gcgctggcaa
     1561 aaaagaagaa aaacatgctg gagtatcagg agatcagcga cttcttttcc gacatgcagc
     1621 tggatgcaga gcagtttgag aagattcttg atttccttga ggcaaacaac atcgacgtgc
     1681 ttcgtattac ggatgacgac gttgacgatg agatgcttct tgacgtcgat gatgaggatg
     1741 agattgaggt cgagaagatt gatctttccg ttccggacgg tgtctccatt gaagatccgg
     1801 tgcgta
//
LOCUS       DBMDQJ010000535         1802 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21951_length_1802_cov_1.597884, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000535 DBMDQJ010000000
VERSION     DBMDQJ010000535.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21951_length_1802_cov_1.597884"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="ACZO9R_10045"
     CDS             <1..1122
                     /locus_tag="ACZO9R_10045"
                     /inference="COORDINATES: protein motif:HMM:NF025314.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4214 domain-containing protein"
                     /protein_id="HIR0913438.1"
                     /translation="RDNYQNAQLYVTRLYESLLEREPDASGMNAYATAISEETMTAAD
                     VAVDIISSSEFKNKNYTHEVYVRKLYAALFARSPQDSEVSNWVEVLSNGVSQKYVLKQ
                     LIESSEFATVCSYYMFSPGTVSLTENRDQNYNATAYVMRCYRKILSRDADVSGLNTWT
                     GKLVAGNGGAEIVKDLVMSEEFRNLNKSDAEFVDILYAAMLDRSSDESGKNTWLSTLN
                     DGVSYVYVINGFAGSTEFGNICSGYGITPGQAEITEARDKNIKVTQYVNRCYEKALGR
                     TGETDGINYWCSIILSGAQSPKDVAYGFVFSQESENQNRNNADYTEMLYNLCLGRASE
                     AAGKADWVGRLEQGTSREEVYWGFANSEEFENIIASYGL"
     gene            1147..>1802
                     /locus_tag="ACZO9R_10050"
     CDS             1147..>1802
                     /locus_tag="ACZO9R_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913439.1"
                     /translation="MKKRIAAILLLGCMVMGSVGTPAQVSAAEVAETVTEEETEQTSE
                     ETSVDTAAAESTETAENTETAENTETAESTESAEPVKAAAADTTENETEATAEEITHP
                     TGLLDLNVINVGTIAEDDILDETEANSSKSSSVYAAAWDAYSSNYVYNMLSTEERKFW
                     DGLDASCRQVLLGTDNLTDGHIPYVSCGSLNSNRITYVAYMFRYMNPQYYFINVSLYQ
                     G"
BASE COUNT          519 a          353 c          525 g          405 t
ORIGIN      
        1 agagataatt atcagaatgc acagctgtat gtgaccaggc tgtatgagag ccttctggag
       61 cgtgagccgg atgcaagcgg catgaatgca tatgccactg caatttcgga ggagacgatg
      121 accgctgccg atgttgcggt tgatatcatt tccagcagtg aatttaagaa taagaattac
      181 acgcatgagg tgtacgtgcg gaagctttac gcggcacttt ttgccagaag tccgcaggat
      241 tcggaagtca gcaactgggt ggaggttctg tccaacggag tctcacagaa atatgtgtta
      301 aaacagctga ttgagtcgtc agagtttgcg acagtgtgct cctattatat gttttcaccg
      361 ggaacggtga gcctgacgga gaaccgtgat cagaactaca atgcgacggc gtacgtgatg
      421 cgctgctacc gcaagatcct tagcagggat gcggatgtgt ccggtctgaa tacatggaca
      481 ggcaagctgg ttgccggtaa cggtggagct gagatcgtaa aagatctggt aatgagcgaa
      541 gagtttcgta atctgaacaa gtctgatgcg gaatttgtgg acatcctcta tgcggcaatg
      601 ctggatcgaa gctcggacga gtccggaaag aatacctggc tttcaacctt aaatgatggt
      661 gtttcctatg tctatgttat caacggtttt gcggggtcga cagaatttgg aaatatctgt
      721 tccggttatg gaatcacacc gggacaggcg gagatcacag aggcgagaga caaaaatatc
      781 aaggtgacgc agtatgtcaa ccggtgctat gagaaggcgc ttggaagaac cggagagacg
      841 gacggcatca attactggtg ttccatcatt ctttcggggg cacagtcgcc gaaggatgtg
      901 gcgtacggat ttgtattttc gcaggaatct gaaaaccaga accgaaacaa tgcggattac
      961 acagaaatgc tttacaacct gtgcctgggc agagcatctg aggctgccgg aaaagcagac
     1021 tgggtcggcc ggctggagca gggaacttcc agagaagagg tatactgggg atttgcgaac
     1081 tcggaagagt ttgaaaatat catcgcaagc tacggtctgt aatgatcata aaaaggagta
     1141 ttaggtatga agaaacgaat tgcagcaata ctgctgttgg gatgcatggt aatgggaagt
     1201 gtcgggacac cggcacaggt atccgcagca gaagtggcag aaactgtaac ggaggaagaa
     1261 acagagcaga catctgaaga gacgtctgtg gacaccgccg cagcagagag tacggagacc
     1321 gcagaaaata cggagactgc ggaaaatacg gagactgcgg aaagcacgga gtctgcggag
     1381 cctgtgaaag cggcagctgc cgacacaaca gagaacgaga cagaagcgac cgcagaggag
     1441 attacacatc cgacaggttt gctggatctt aacgtgatca atgtcggaac gattgcagaa
     1501 gatgatattt tagacgaaac agaggcaaac agcagcaaga gttccagtgt atatgcagca
     1561 gcgtgggatg cttattcgtc caattacgtg tataatatgc tttcgacgga agaacgcaaa
     1621 ttctgggatg gactggatgc atcctgcaga caggtgcttc ttggtacgga taatctgacg
     1681 gatggacata taccgtatgt atcctgcgga agcctgaatt caaacagaat tacgtatgtg
     1741 gcatatatgt tccgttacat gaatccacag tattatttta tcaatgtgtc tttgtatcag
     1801 gg
//
LOCUS       DBMDQJ010000536         1802 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_21955_length_1802_cov_1.408583, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000536 DBMDQJ010000000
VERSION     DBMDQJ010000536.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21955_length_1802_cov_1.408583"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            52..551
                     /gene="tadA"
                     /locus_tag="ACZO9R_10055"
                     /pseudo
     CDS             52..551
                     /gene="tadA"
                     /locus_tag="ACZO9R_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005345192.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
     gene            complement(545..>1802)
                     /locus_tag="ACZO9R_10060"
     CDS             complement(545..>1802)
                     /locus_tag="ACZO9R_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913440.1"
                     /translation="GTEPMSGVAIVNQLFFVFNICIFGGVSGAGIFSAQFFGKGDFEG
                     QKYTFRFKLYACLLITAIACVLFHFLDEPLISLYLNDGGSVGNTRLALSYGKEYLAIM
                     IFGLLPFAVSQTYVSTIRETGQTFVPMVSGIVAVITNLVLDYVLIFGAFGAPELGVAG
                     AAIATVIARYTECLIVVVWAHRHLYKNPYLIGVYKGLRIPGSILVDIFRKGLPLMFNE
                     MLWAVGMAVIVQCYAVRGLEVVAAQNISSTISNLFNIVYLQLGNCISIVVGQKLGAGQ
                     LEEAKDADNKIIFFDVACCACISVIMILLGGLFPEIYKTEPGIKALAKNFIIISAMAM
                     PLCAFSHCSYFTLRSGGKTIVTFLFDSVYTWVVMIPYAFVLSRFTTLSITMVFFLVSF
                     TEIIKVIIGFFMIKSNVWLQNIVNSY"
BASE COUNT          588 a          382 c          459 g          373 t
ORIGIN      
        1 gaccaggagg ttctggtgaa aacaacacaa ctaaccgacc aggaaaagta catgaaggaa
       61 gcattacggc aggcgaagaa ggcctatgca ttgggtgagg taccgattgg ctgcgtgatc
      121 gtgcatgaag gaaaaattat cgggagagga tataaccgtc ggaatacgga taaaaataca
      181 ttggcgcatg cggaaattac cgcaatcaat aaggcaagca ggaaattagg tgactggagg
      241 cttgagggat gtacgctgta tgtgacgctg gagccttgtc agatgtgcgc cggggcgcaa
      301 gaatcgatga agtggttatg gccagtatga atccgaaggc aggctgcgga ggatccatct
      361 taaatattct ggaaatgcct gagtttaatc atcaggtgaa ggtgacaagg ggaattttgg
      421 aagaagaatg ctcccatatg ctgacacagt tctttaaaga gctccgtatc cgcaacaagg
      481 cagaaaagga actccgtaaa gaaaaggatc agcagaagaa tctgaatgag gatttatcat
      541 ccggtcaata agaattcaca atgttctgca gccagacatt actcttaatc ataaagaagc
      601 cgatgatgac cttgatgatt tctgtaaagc tgacaaggaa aaataccatc gtgatggaaa
      661 gcgtggtaaa gcgtgataga acgaaggcat agggaatcat gaccacccag gtataaacac
      721 tgtcaaacag gaaagtgacg atggttttgc cgccggaacg cagggtaaaa tagctgcagt
      781 gggagaaggc acacagaggc atcgccattg cagaaataat aatgaagttt ttggcaagag
      841 ccttgatgcc gggttctgtc ttatagattt cagggaaaag accgccgagc aggatcataa
      901 tgacagagat acaggcacaa cacgccacat caaagaagat gatcttatta tcggcatcct
      961 ttgcttcctc gagctgaccg gctcccaatt tctgaccaac cacaattgaa atacagtttc
     1021 caagctgcag atagacaata ttaaacagat tggaaattgt ggaggaaata ttctgcgcag
     1081 caaccacctc gagaccccgt acagcatagc attgtacgat gacggccatg ccaaccgccc
     1141 acagcatttc attaaacatt aagggcagtc ccttacggaa aatatcgaca agaatggatc
     1201 cggggatgcg taatccttta tacacaccaa taagataagg attcttataa aggtggcggt
     1261 gagcccagac aacaacaatc aggcattccg tgtaacgggc gattacagtg gcaatggcag
     1321 ctccggctac accaagctcc ggtgctccga aggcaccgaa aatcagtaca taatcaagga
     1381 ccaggttggt aatcaccgca acaataccgg aaaccatcgg cacgaaggtc tgtccggttt
     1441 cacggatcgt gcttacgtag gtctgggaaa cagcaaaggg gagcaggcca aagatcatga
     1501 tggcgagata ttctttgcca taggacaggg caagccgggt attaccgaca gaacctccgt
     1561 catttaagta gagggaaatg agcggttcat ccaggaaatg aaacagcaca caggcaatcg
     1621 cagtgatcag cagacaggcg tacaatttga aacggaaggt atatttctgt ccctcaaagt
     1681 ctccttttcc gaaaaactgc gcactgaaaa tacctgcacc ggagacgcct ccaaagatgc
     1741 agatattaaa tacaaaaaat aactgattta caatggcaac gccggacatg ggttctgttc
     1801 cg
//
LOCUS       DBMDQJ010000537         1797 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22032_length_1797_cov_1.303066, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000537 DBMDQJ010000000
VERSION     DBMDQJ010000537.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22032_length_1797_cov_1.303066"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            125..1797
                     /gene="feoB"
                     /locus_tag="ACZO9R_10065"
                     /pseudo
     CDS             125..1797
                     /gene="feoB"
                     /locus_tag="ACZO9R_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006857960.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
BASE COUNT          452 a          385 c          517 g          443 t
ORIGIN      
        1 ttttagtgtt ccctgaaaca aaaaattcca ttacaccgga atgccggttg acaatgcaga
       61 agcatcatat tagtataagt gagaatgaat atcaatatca aaagagaaaa ggtgtgtgga
      121 aaatatgaca ttagaggaac tgccaatcgg aaagcgcgct gctattactg cggtcggcgg
      181 agagggcaca ctgcggtgcc ggctgttaga tatgggactg atcccgaaga ctgctgtgga
      241 ggtgcgcaag gtggctccga tgggagatcc gattgaaata cgcatccgcg ggtatgagct
      301 tacgatccgg cgggaggacg cagcgaaaat tgaagtgaaa ccggaggaag aaaaatgata
      361 tttgcattgg ctggaaacca gaactgcggt aagacgacgt tgtttaatca gctgacagga
      421 gctaaccagc atgtcggcaa tttccccggc gttacggtgg atcagaagat gggggaaatc
      481 cgctccacga agaattgttc cgtggtagat ctccccggaa tctattccat cagaccttat
      541 accaatgagg agattgtcac cagagatttt atcttaaatg agaagccgga tggaatcatc
      601 aacattgtgg atgcgacgaa catcgagaga aatctttacc tgacgctcca gcttctggaa
      661 atgcacattc cgatggtgtt agcactcaat atgatggatg aggtccgcgg aaacggaggt
      721 tccattgatt acaggcagat gtcgcaggaa ttagggattc ccgtgattcc gatcagtgcg
      781 gcgaaggatg agggaattga ggatctgatt gcggcgattg tggaagttgc gggcaaaaag
      841 ataccgccca aagtcatgga tttctgcgag caggggccgg tacaccgctg cattcatgcg
      901 gtgtcgcatc aggtggagga tcatgcggta aatatcggga tgtccccgcg ctttgcggcg
      961 acgaagatcg tggaaaatga cacggacatt atagaacgtc tggctctttc tgagaatgag
     1021 ctggaaatga tggaacatag tattgcggag atggaaaaag actgtggatt ggaccggaat
     1081 gcggcgttag ctgacatgcg gtataccttt atagagaagg tgtgccaaaa aacggttaaa
     1141 aaatgtcagg agagcaggga gcacatccgg agtgtgaaaa tcgacggtgt gctcacgaac
     1201 aagtaccttg cgatcccgat gtttctactg atcatgtttt tgattttctg gctgacgttc
     1261 catgtggtcg gcgcggcgct aagcgactgg ctggcggtgg gaatcgacgc gtttactgcc
     1321 gtctgcgaca ggggacttac cgcgtacgga ctgaatccgg ttgtgcacag cctgctcatc
     1381 gacggtgttt ttgccggcgt cgggagtgtg ttaagcttcc tgccgatcat cgtggtgctg
     1441 tttttcttcc tctcgattct ggaggactcc ggctacatgg cgcgcgtggc tttcgtgatg
     1501 gacaagccgc tgcgtaaaat cggactttcc gggcgaagct ttgtgccgat gctgatcgga
     1561 ttcggctgta cggttccggc ggtcatggca tcccgtgcgc tggcaaccga gcgggaccgc
     1621 cgccgcacca ttctcattac accgttcatg tcctgctctg caagacttcc gatttatgta
     1681 ttattttcct ctatgttctt cggaaaatat gccatgctgg tctgttattc catgtatctt
     1741 ttgggaatcg ctgtagcaat cataaccaca tttattctat ctaaaataga tggaagc
//
LOCUS       DBMDQJ010000538         1797 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22036_length_1797_cov_0.835495, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000538 DBMDQJ010000000
VERSION     DBMDQJ010000538.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22036_length_1797_cov_0.835495"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(43..642)
                     /gene="recR"
                     /locus_tag="ACZO9R_10070"
     CDS             complement(43..642)
                     /gene="recR"
                     /locus_tag="ACZO9R_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR0913441.1"
                     /translation="MEYYSSQISQLVEQLASLPGIGAKTAQRLAFHIINMPEEQVESL
                     SAAITDAKKNIRYCKSCYTITDQELCPVCASPKRNHKLIMVVETPRDLAAYERVGQYQ
                     GVYHVLNGVINPAMGIGTGDIRLKELILRLEQEDVEELIIATNSSVEGEATAMYISKL
                     VKPSGIKVTRIASGVPVGGDLECIDEVTLLRALEGRTLL"
     gene            complement(642..986)
                     /locus_tag="ACZO9R_10075"
     CDS             complement(642..986)
                     /locus_tag="ACZO9R_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR0913442.1"
                     /translation="MAKRGGFPGGGMPGNMSNLMKQAQRMQRQMEESAKELETAEFTA
                     TAGGGAVEVPVTGKKEVTKVKLDPEAVDPDDVEMLEDVIMAAVNEALRKADEASAANM
                     AKLTGGLGGFGL"
     gene            complement(1006..>1797)
                     /locus_tag="ACZO9R_10080"
     CDS             complement(1006..>1797)
                     /locus_tag="ACZO9R_10080"
                     /inference="COORDINATES: protein motif:HMM:NF023591.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR0913443.1"
                     /translation="ICDQKLTDVVRELEQIILQGRELSQFVTDFIWYLRNLLMLKSDQ
                     NMEDVLDVSTERLAQMKKEADLTDTDTLMRYIRVLSELSGQLRYATQKRVLVELAMIK
                     LCRPAMEQNYDSILQRIRVLEQQMEKGIPMMAAVESGAVQSRQETESRSQEKLPREAL
                     STDVRMFVDRFQELRRKMGGMQSQQLKDAALRELPDGTGVQIILARELPVDEQELADS
                     VEKMIERELKVRMKVQIHTPHTDPRPQDPVTDLSGFIDMDIDIEP"
BASE COUNT          387 a          537 c          332 g          541 t
ORIGIN      
        1 gattctcttc atactgtaac ctccttaatt tatgtgtctc ctctacaaca aagttcttcc
       61 ttccaatgcc cgaagtaacg tcacttcatc aatacattcc agatcgccgc cgaccggaac
      121 gccacttgca attcttgtga ccttgattcc agatggcttg accagcttgg atatatacat
      181 cgcagtcgct tcgccctcca cactggaatt tgttgcaata atcaactctt ctacatcttc
      241 ctgctccaac cgcaggatca gttccttcaa ccggatatct ccggttccaa ttcccatcgc
      301 cgggttgatc acaccattta atacatggta gacgccctga tactgtccca cacgctcata
      361 tgctgccagg tctcgtggag tctcgacaac catgatcagc ttatggttcc gcttcggcga
      421 tgcacacacc ggacacagct cctgatcagt aatcgtataa caggatttgc agtaccggat
      481 attcttcttt gcatctgtga ttgctgccga caggctttcc acctgttctt ccggcatatt
      541 gataatatgg aacgccagcc gctgtgcggt ctttgctccg atacccggca aagaagccag
      601 ttgttctacc aactggctga tctgactgct gtaatattcc atcagagacc aaatcctccc
      661 agccctccgg taagcttggc catatttgct gctgatgctt cgtctgcttt gcgcagtgcc
      721 tcattgaccg ctgccatgat cacatcttcc agcatctcta catcatccgg atcaactgct
      781 tccggatcaa gtttcacttt tgtcacttct tttttaccgg ttacaggcac ttctactgct
      841 ccgccgcctg cggttgctgt aaattctgct gtctccagtt cttttgcact ctcttccatc
      901 tgacgctgca ttctctgtgc ctgtttcatc agattactca tgtttcccgg cattccgcct
      961 cctggaaatc cacctctttt tgccatattt ctatcctcct gttttctatg gttcaatgtc
     1021 aatatccata tctataaacc ctgacagatc cgtcacagga tcctgcggtc ttggatctgt
     1081 atggggtgta tgaatctgta ctttcatgcg gaccttaagt tctctttcaa tcattttttc
     1141 cacgctgtcc gccagttcct gttcatccac cggcagttct cttgccagaa tgatctgcac
     1201 accggttcca tccggcagtt ctcgcaaagc cgcatccttc aactgctggc tctgcattcc
     1261 acccatcttt ctgcggagtt cctgaaagcg gtctacaaac atccgcacat ctgtagataa
     1321 agcttctctt gggagcttct cctgactgcg ggactctgtc tcctgccggg actgtaccgc
     1381 tcctgattcc actgctgcca tcataggaat ccctttttcc atctgctgtt ccagcacacg
     1441 gatcctctgc aaaatagaat cataattctg ttccattgcc ggacggcaca gcttgatcat
     1501 ggccagttcc acaagcacac gcttctgcgt cgcataacga agctgtcctg ataattctga
     1561 caacacacga atataacgca tgagcgtatc cgtatctgtc agatctgctt cttttttcat
     1621 ctgtgccaga cgttccgttg atacatccag cacatcttcc atattctgat cgctctttaa
     1681 catcagaaga ttgcgaagat accagataaa atctgtgaca aactgagaca gttcccgacc
     1741 ctgcagaatg atctgttcca gttcccgcac cacatctgta agcttctgat cacagat
//
LOCUS       DBMDQJ010000539         1794 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22077_length_1794_cov_1.243355, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000539 DBMDQJ010000000
VERSION     DBMDQJ010000539.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22077_length_1794_cov_1.243355"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1794)
                     /locus_tag="ACZO9R_10085"
     CDS             complement(<1..>1794)
                     /locus_tag="ACZO9R_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR0913444.1"
                     /translation="RNARNFWCHIGTHGSWSATGNSAEEIAKKFTDLADENEVTAGFM
                     WQTVTRTSRKYQLSAQITSFVPLDDPMEIMYVEITNTGNMQVRFTPTAAIPVYGRSAD
                     NLRDHRHVTSLLHRIRTVENGVLVTPTMSFDERGHRPNEMTYFVCGYDQTGEAPEDFY
                     PTVQDFIGEGGNFERPKAVVKNLAGVPAGSAYAGCEAVGGLHFKERILAPGETAGYTI
                     MIGAVRKTEHAGETILSMTAAYRTAAQTKAAYERMKTYWEQKVNVSYHTKDRQFDRYM
                     QWVSFQPILRRIYGCSFLPHHDYGKGGRGWRDLWQDCLALLLMNPDGVREMLLNNFQG
                     VRIDGTNATIIGSKEGTFIADRNQITRVWMDHGVWPFVTTKLYIDQTGDLAVLEEEIP
                     YFKDQQAGRGTKKDAAWKEQDGCWQRDAAGNVYRGSVLEHLLLQNLTAFYEVGEHGHI
                     RLRGADWNDALDMAGERGESVAFTNAYAGNLRELAELLALYAEKTGKESVPLNRDLFV
                     LLADDDTLYASVQKKQELLTRYTDACIHEVSGQKTEVSITELVRSLQKKAAFLTGHIR
                     ATEWVTDEEGNGWFNGYYDNHGRCVEGVFAQ"
BASE COUNT          356 a          572 c          426 g          440 t
ORIGIN      
        1 cctgcgcaaa gacaccctcc acgcacctgc catgattgtc atagtatccg ttaaaccagc
       61 cgtttccctc ctcatcggtc acccactctg tggcacggat atgtccggtc agaaaggctg
      121 ctttcttctg caggctgcgg acaagctctg taatcgaaac ctctgtcttc tgcccgctta
      181 cttcatggat gcatgcatcg gtatagcgcg ttaagagttc ctgctttttt tgaaccgatg
      241 catacagggt gtcgtcatcc gcaagcagca cgaaaagatc ccgatttaac ggcacagact
      301 ccttcccggt tttctctgcg tagagtgcaa gcagctccgc gagttcccgg agattgcctg
      361 cataggcgtt ggtaaatgca acgctctccc cgcgctcgcc cgccatatcc agcgcatcgt
      421 tccagtccgc gccgcgaagg cggatatgtc cgtgttcgcc cacctcgtag aaagccgtca
      481 gattctgcag cagaagatgc tccagcacgc ttccgcggta gacatttccc gcggcatccc
      541 tctgccagca gccgtcctgc tccttccatg ccgcatcctt ctttgtcccg cgccctgcct
      601 gctgatcctt aaaatatggg atctcttcct ccagaaccgc aagatcgccg gtctggtcga
      661 tatagagctt tgtcgtcaca aacggccaga ccccgtgatc catccacacg cgcgtaatct
      721 gattgcggtc ggcgataaag gttccctcct tgcttccgat aatggtcgca ttggttccgt
      781 cgatgcggac accctgaaaa ttattgagaa gcatctcgcg cacaccgtcc ggattcatca
      841 aaagaagtgc cagacagtcc tgccagagat ctctccagcc tcttcctccc tttccgtaat
      901 catggtgcgg caaaaaggaa cagccataga tccggcgcag gatcggctga aagctgaccc
      961 actgcatata gcggtcaaac tggcggtctt tcgtgtggta ggatacgttt actttctgtt
     1021 cccagtatgt tttcatgcgc tcatacgccg ccttcgtctg cgccgccgtg cggtacgctg
     1081 ccgtcatgct taaaatcgtc tccccggcat gttccgtctt tcgcaccgcc ccgatcatga
     1141 tcgtgtatcc tgcggtctcg cccggtgcaa gaattctctc cttaaaatgg agtccgccga
     1201 ccgcctcaca tccggcatat gcactgcccg ccggcacacc ggcaagattt ttgacaaccg
     1261 cctttggccg ctcaaaatta cctccctcgc cgataaaatc ctgcacggtc gggtaaaaat
     1321 cttccggtgc ctcccccgtc tggtcgtatc cgcacacaaa gtaggtcatc tcgttcgggc
     1381 ggtgcccccg ctcgtcaaaa gacatcgtcg gcgtcacaag cacaccgttc tccaccgtgc
     1441 ggatgcggtg cagcagcgat gtcacatgcc ggtgatccct cagattgtcc gcgctcctgc
     1501 catagaccgg tatggctgcg gtcggcgtga atcgcacctg catgttgcct gtattggtaa
     1561 tctccacata catgatctcc atcggatcgt ccagcggaac aaacgatgtg atctgtgccg
     1621 aaagctgata cttcctggag gttctcgtca cggtctgcca cataaaaccg gctgtcactt
     1681 cgttttcatc cgcaagatcc gtaaattttt ttgcgatttc ttccgccgaa ttgcccgtgg
     1741 cggaccagct tccgtgcgtt ccgatatggc accagaaatt tctggcattc cggt
//
LOCUS       DBMDQJ010000540         1786 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22182_length_1786_cov_1.741246, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000540 DBMDQJ010000000
VERSION     DBMDQJ010000540.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1786)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1786)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1786
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22182_length_1786_cov_1.741246"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..>1786
                     /locus_tag="ACZO9R_10090"
     CDS             <1..>1786
                     /locus_tag="ACZO9R_10090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR0913445.1"
                     /translation="PWYEHHKATEETLRRQRKQSAAWKGAPCVSIVVPLYCTNETFLR
                     EMIGSVQAQSYENWELCLADGSPEENAARLEAVVRKCAGEDARICYRRLEKNLGIAGN
                     TNAAIAMAHGEWIALLDHDDLLAPDALYETVHLILRGPKDETGVAATGFRKAGAQYDM
                     IYTDEDKVDMDGKTHFQPHFKPDINIDLLRSNNYITHFTMMRKTLLEKAGMIREEYDG
                     AQDYDLFLRCVECAEAIGHVPRVLYHWRCHTESTSENPFSKQYAVDAGRRAIEDHLKR
                     QGIAGEVTATKDMGFYTVRYPYSGNPLVSIVIPSRDEVETLKKCLAAIQKTQYANYEV
                     IVVENNSAPETFDFYRSITSEEVKEGEAVCYEGTLSGGQRICVAVWKEGFNYSALNNF
                     GVSHAKGEYLVLMNNDIEMITEDWLEEMLGTCQRREVGVVGAKLYYPDDTVQHAGIVV
                     GIGGNARGIGQNMFMGLQRARSGYLHKASLAMDYSAVTAACLMVKRSIYEAVGGFEEK
                     LAVAFNDVDFCLKVRKEGYLVVYQPRVEAYHYESRSRGSEDSPEKVARFGREIEYMRN
                     HWIGILKNGDPYYNPNFSTVYPNYALRDY"
BASE COUNT          443 a          405 c          570 g          368 t
ORIGIN      
        1 ccatggtatg agcatcacaa ggcgacggag gagacgctgc gccgtcagcg gaagcagtcc
       61 gccgcgtgga agggagcacc ctgcgtcagc atcgtggtgc cgctctattg taccaatgag
      121 acattcctgc gggagatgat cggttcggtg caggcgcaga gttacgaaaa ctgggaactc
      181 tgcctggcag acggaagtcc ggaggaaaac gctgcgcgtc ttgaggcggt cgtgaggaaa
      241 tgtgccggag aggatgcgcg catctgttac cgcagactgg aaaaaaatct tggaatcgcc
      301 ggcaatacca atgcggcgat tgcaatggcg cacggggagt ggattgcact tttagatcat
      361 gacgatctgc tggcgccgga cgcactgtat gagaccgtgc atctgattct gcggggaccg
      421 aaggatgaga ccggagttgc agcgacaggt tttagaaaag ccggtgcgca gtatgacatg
      481 atctacacgg atgaagacaa ggtggacatg gatggaaaaa cgcatttcca gccgcacttt
      541 aagccggaca tcaacatcga tctgcttcgt tccaacaact atatcacgca ctttaccatg
      601 atgcggaaaa cgcttctgga gaaagccggc atgatccgtg aggagtatga cggtgcgcag
      661 gattacgacc tgtttctgcg ctgcgtggaa tgcgcagagg ccatcggaca tgtgccgcgg
      721 gtgctctacc actggcgctg ccacacggag tcgacgtcgg aaaacccgtt ttccaagcag
      781 tatgcggtgg atgcgggacg gcgcgcgatc gaggatcatc tgaagcgtca ggggattgcg
      841 ggggaagtga cggcgacaaa ggatatgggc ttttacacgg tgcgctatcc ttacagcggc
      901 aatccgctgg tctcgatcgt gataccgagc agggatgaag tggagacgtt aaaaaaatgt
      961 cttgcagcca ttcaaaaaac gcagtatgca aattatgaag tcattgtggt ggagaataac
     1021 agcgctccgg agacatttga cttttaccgc tcgatcacat ccgaagaggt gaaagagggc
     1081 gaagcggtgt gctatgaggg aaccttatcc ggcggacagc ggatctgcgt ggcagtctgg
     1141 aaggaagggt ttaattattc ggcgcttaac aatttcggcg tctctcatgc aaagggcgaa
     1201 tatctggtgc ttatgaacaa tgacatcgag atgattaccg aagactggct cgaagagatg
     1261 cttggcacct gccagcgcag ggaggtcggt gtcgtgggag cgaagctgta ttatccggat
     1321 gataccgtgc agcatgcggg aatcgttgtc ggaatcggcg gcaatgcgcg cggcatcggg
     1381 cagaatatgt ttatgggact gcagagagcg cgcagcggat acctgcacaa ggcgtcgctt
     1441 gcgatggatt actcggcggt cacggcggca tgcctgatgg taaagcggag tatctacgag
     1501 gcggtgggcg gatttgagga aaagcttgcc gtggcgttca atgatgtgga cttctgtctg
     1561 aaagtccgca aagaggggta ccttgtggtg taccagccgc gggtggaagc ctatcactac
     1621 gaatccagat cgcgcggaag cgaggattcc ccggaaaaag tggcgcgctt cggacgggag
     1681 atcgaatata tgcggaatca ctggatcggc attttaaaga acggtgatcc ctactacaat
     1741 ccaaactttt caacggtata tccgaattat gcgctgagag attatc
//
LOCUS       DBMDQJ010000541         1781 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22260_length_1781_cov_1.191071, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000541 DBMDQJ010000000
VERSION     DBMDQJ010000541.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22260_length_1781_cov_1.191071"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="ACZO9R_10095"
     CDS             <1..675
                     /locus_tag="ACZO9R_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012741466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR0913446.1"
                     /translation="ETNGQSFGPVHRQQSSGKTGWELDQELQQIYAREFGMSREDMED
                     QERRKWLKKKSDAPKPNVVKYDKKGNPIYPAKEPQEEYLIVDGYNIIFAWKDLNELSR
                     VNIDSARDKLLDILSNYQGYKSCPVLVVFDAYKRKEHPGAKSKYHNLDVVYTKTDETA
                     DAFIERTVHEIGHKYRVTVATNDGLEQLTVMSQGALRMSADNLREEIERLSRLEYENY
                     LASQKR"
     gene            756..>1781
                     /locus_tag="ACZO9R_10100"
     CDS             756..>1781
                     /locus_tag="ACZO9R_10100"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015515609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR0913447.1"
                     /translation="MFVAEDMYQLGSQRSAIRELFEYGKQRAAVVGAENVYDFSLGNP
                     TVPAPDCVNETIRDLTQKLDSISLHGYTSAQGDIETRQAIADYLNKTYETAFSADNFY
                     LTMGAAASLSVCFRALTTSPEDEFITIAPFFPEYRVFVEANGAKLVVVPPHTSDFQID
                     FEALEKAISAHTKGVIINSPNNPSGAVYSKETIRKLAALLTKKSEENGTPVFILADEP
                     YREIAYDGVEVPYVTKYYANTLVCYSYSKSLSLPGERIGYVIVPDEVTESKTVYAAIA
                     GAGRALGYVCAPSMFQKVIASCVGNTGDIELYRKNRDLLYDGLTKIGYECFKPQGAFY
                     MFVKALES"
BASE COUNT          501 a          388 c          482 g          410 t
ORIGIN      
        1 gagaccaacg ggcagagttt tggcccggtg caccggcagc agtcttccgg caagaccggc
       61 tgggagttag atcaggagct gcagcagatt tatgcccgcg agtttggcat gagccgcgaa
      121 gatatggaag atcaggaacg gcgcaagtgg ctgaaaaaga aatccgatgc gccgaagccg
      181 aatgtggtaa aatatgataa aaaaggcaat ccgatctatc cggcgaagga accgcaggaa
      241 gaatatctga ttgtggacgg ttacaatatt atttttgcat ggaaagactt aaatgaactt
      301 tctcgtgtca atattgattc cgcaagggac aagcttttgg atattttaag caattatcag
      361 ggatacaaga gctgtccggt gctggtggtg tttgatgcct ataagcgcaa ggaacatccg
      421 ggtgccaaaa gcaaatatca caatctggat gtggtctaca cgaaaaccga tgagaccgcg
      481 gatgcattta tcgagcgcac cgtgcatgag atcgggcata agtaccgtgt cacggttgcg
      541 acaaatgacg ggctggagca gcttacggtc atgagtcagg gggcgcttcg catgtcggcg
      601 gacaatctcc gggaggagat cgagcggctg agccggctgg agtatgaaaa ttatctggca
      661 agtcagaagc gataacttga aaaacaggag atataaaagg ttgaaaagcc ttgaatttta
      721 atagagacac ctgattatat gaggaggaaa ataaaatgtt cgtagcagaa gatatgtatc
      781 aattaggaag ccagcgttcg gcaatccgtg aattatttga gtatggcaag cagcgcgcag
      841 cagttgtggg agcggaaaat gtctatgatt tttcccttgg aaacccgacc gtaccggcac
      901 cggactgcgt caatgagacc atccgtgacc tgacacagaa actggattcc atttctttgc
      961 acggatatac ttctgcacag ggagatatcg agacgagaca ggcaatcgca gattacctga
     1021 acaagacata tgagactgcg ttttcggcag acaactttta cctgactatg ggagctgccg
     1081 catccttgtc ggtatgtttc cgcgcactta ccacttcacc ggaagatgag tttatcacga
     1141 ttgcaccgtt tttcccggag taccgtgtgt ttgtggaagc caacggagcg aaacttgttg
     1201 tggtaccgcc tcacaccagc gatttccaga ttgattttga agccttagaa aaagccatca
     1261 gcgcacacac caaaggcgtg atcatcaact ccccgaacaa cccgtccgga gctgtctatt
     1321 cgaaagagac gatccggaaa cttgctgcac ttctgacaaa gaaatccgaa gagaacggca
     1381 caccggtctt tatccttgcg gatgaaccgt accgtgagat cgcatacgat ggcgtggaag
     1441 taccgtatgt gacaaaatat tatgcaaaca cactggtatg ctattcctac agcaaatccc
     1501 tgtcgctgcc gggtgaacgc atcggctatg tgattgttcc agatgaagtg accgagagta
     1561 agacggtcta tgcggcaatt gcaggagccg ggcgtgcgct gggttatgtc tgtgcaccga
     1621 gcatgttcca gaaagtgatt gcatcctgtg tcggcaacac tggggatatc gaattatata
     1681 ggaaaaaccg cgatctgctc tacgatggcc tgacaaagat cggctatgaa tgttttaagc
     1741 cacagggcgc tttttacatg tttgtcaaag cattggagtc g
//
LOCUS       DBMDQJ010000542         1777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22318_length_1777_cov_1.584726, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000542 DBMDQJ010000000
VERSION     DBMDQJ010000542.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22318_length_1777_cov_1.584726"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(100..765)
                     /gene="sdaAB"
                     /locus_tag="ACZO9R_10105"
     CDS             complement(100..765)
                     /gene="sdaAB"
                     /locus_tag="ACZO9R_10105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HIR0913448.1"
                     /translation="MNLLDIIGPVMVGPSSSHTAGAVKIGRVSRKLLAEEVADAKIYF
                     HGSFLATGKGHGTDKAILAGLLGMQVDDPRIPESFTMAKESGMSFTLEGIDLGDVHPN
                     SVKMNLTGKSGRTLEVIAASVGGGQIRICELDGLTANFSGDYPTLIVHNIDQPGHVAE
                     VTSMLAHKSVNIATMQLYRESRGTRAVMVIECDREVPQESIEWLEHLEGILKVTYLSM
                     LEA"
     gene            994..1395
                     /locus_tag="ACZO9R_10110"
     CDS             994..1395
                     /locus_tag="ACZO9R_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HIR0913449.1"
                     /translation="MTHTHVDGTTHTHTDGTTHTHADDTVHTHASGSVHTHAHGSTHT
                     HPHHHENTKAVLNRMNRAIGHMEAVRTMIEDDRDCSEVLVQIAAVRSAINNIGKIILE
                     DHISHCLVDAVETGDEQAIRDLNDAIDKFVK"
BASE COUNT          449 a          512 c          348 g          468 t
ORIGIN      
        1 atcggtacat tgtcatcctc cctctgtttt tctatcatcc agacggctcg caccgtcttc
       61 ccgtcacata cgctgcatcc ttctgtccac cggatgtctc tatgcttcga gcatgcttaa
      121 ataggtaacc tttaaaattc cctccagatg ctccagccac tcgatcgact cttgcggcac
      181 ctctctgtca cactcgatga ccataaccgc cctggtgccc cggctctcgc ggtaaagctg
      241 catcgtagcg atatttaccg acttgtgcgc cagcatcgaa gtcacctcag ccacatgccc
      301 cggctggtca atgttgtgca cgatcaaggt cggataatcc ccgctgaaat tcgctgtcag
      361 cccatccagc tcgcagatcc ggatctgtcc gccgcctacc gacgccgcaa tgacttccag
      421 cgtacgcccg gattttccgg tcagattcat cttgaccgag ttgggatgta catctcccag
      481 atcaatcccc tccagtgtaa aactcatccc agactccttt gccatcgtaa agctctccgg
      541 gattctcgga tcatccacct gcatcccgag cagtcctgcc aggatcgcct tatccgtacc
      601 gtgtcccttg cccgttgcca gaaaagagcc atgaaaatat atttttgcgt ccgcaacctc
      661 ctccgctaaa agctttctgc tgactctgcc gatcttcaca gctcccgctg tatgtgaact
      721 tgacggtcct accatcacag gtcctatgat gtcgagtaaa ttcatccttt ttctattcct
      781 tcctttgcgc ttttcgcgta aaataagggg tatatccccg atataccccc ttgtctgcca
      841 aattctttcc ttattatata agcattgtac aaaaaagaca acctgttttt tttcgggaga
      901 atatgataca ctgaatgaaa tgcaacgaat cggaggaatc ccatgaaaac acagaagact
      961 tccacagcaa atacacatat acacgccgac ggcatcactc atacgcacgt tgacggcacc
     1021 actcatacac acaccgacgg caccactcac acgcatgctg acgacaccgt tcatactcat
     1081 gccagcggct ccgttcatac tcatgcccac ggctccactc acacacatcc gcatcaccac
     1141 gagaatacca aggcggtctt aaaccgcatg aaccgtgcga tcggacatat ggaggccgtc
     1201 cgcacgatga tcgaagacga cagggactgc agcgaagtgc ttgttcagat tgcggcggtg
     1261 cgttccgcca tcaacaatat cggtaaaatc attttagagg accacatcag ccactgcctt
     1321 gtcgacgccg tcgaaaccgg ggatgagcag gccatccggg acttaaacga cgcaatcgac
     1381 aagtttgtaa agtgatacat ttttcatttg gacatagttt gttcattttt tattcataat
     1441 ttatagaaat tcaggaaatc ttaagccttt tttatacatc ttcccgattg atttttgatg
     1501 caattagcgc tatattctct tagtatcgaa atttagcttt acttcctttt tacaggagtt
     1561 ttacactgga gaatcggcag cccggctgcc cacatatatt gcacgcgtga gcgtatcggt
     1621 gtttcacacc gcctacagtc attttgggag ggttctatga aaaaattatt taacattcta
     1681 aaagaaaagc ttgcaccgct ttccagtaag cttcaatctc tgaagagcat tcttccgcag
     1741 aagaagccac acagtgaaaa atactataaa cggattg
//
LOCUS       DBMDQJ010000543         1777 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22323_length_1777_cov_1.229714, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000543 DBMDQJ010000000
VERSION     DBMDQJ010000543.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22323_length_1777_cov_1.229714"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="ACZO9R_10115"
     CDS             complement(<1..398)
                     /locus_tag="ACZO9R_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR0913450.1"
                     /translation="MAKKETYDESSISVLEGLEAVRKRPGMYIGSVSRKGLNHLIYEI
                     VDNAVDEHLAGACDTIYVTLEADGSCTVEDNGRGVPVGMHAKGVSAARIVYTTLHAGG
                     KFDDSAYKTSGGLHGVGSSVVNALSTYMDV"
     gene            complement(579..>1777)
                     /locus_tag="ACZO9R_10120"
     CDS             complement(579..>1777)
                     /locus_tag="ACZO9R_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913451.1"
                     /translation="KENVYWFCGEDVFIRQIEGERHVYEFLDAYRKRVAEHKELPFMV
                     DALNCAMGCIYGTGVEPEKTAGDDVLYELHKIREKSKKVKGPWARSATPKQRLAQLNR
                     QFAQLEPADFTRRYTDRSKGNEIRVPEGQELEAVYSRLNKQTAMDRAIDCSACGYKTC
                     LDMATAIYNGCNSENSCIHFIKNEAEERGRAAQAAAEESQQANVEIQRRSDLIAGIIE
                     GLNSDFQELDSAIGQMAAGNSSNASESTAITDAMTGVTGFCQEMKQSFEAITELLDKL
                     ENNNNDITKIANQTNLLSLNASIEAARAGAAGRGFAVVAEEIKNLSENSRATANDSND
                     NKNEISQAIGKLLEESDHLTEIIREVDERMTNLAASTEEIAASTDSVKEISSDLKDKA
                     AELMNM"
BASE COUNT          346 a          564 c          383 g          484 t
ORIGIN      
        1 tcgacgtcca tataggtgga cagtgcattc acgaccgagg aacccactcc gtgcagtccg
       61 ccgctggtct tgtatgcgga atcgtcaaac ttgccgcccg catgcagcgt cgtgtacaca
      121 attcgcgctg ccgacacgcc cttcgcgtgc atgccgaccg gcacgcctcg tccgttatct
      181 tccactgtac aggagccatc cgcctccaat gtgacatata tggtatcgca ggcacccgcc
      241 agatgctcgt ccactgcgtt gtccacaatc tcgtaaataa gatgattcag accctttctg
      301 gacacgcttc cgatgtacat tccgggtctc ttccttacgg cttcgagtcc ttccagaacg
      361 gaaatactac tctcgtcata agtctctttc tttgccatgt tcttcttcct ttccttgaca
      421 ttcctctgga taatataaca tactgggaaa acccgtgcaa gcaacagaac gtatgttttg
      481 taaaaatttt ctatttttgt gcaaacagac gaaatacgcg gaaaagaatc cgattgcttt
      541 ctttgtccgc gtattatcat ttgcttttct gttgtatctt acatgttcat cagctcggct
      601 gccttatcct tcagatcaga ggaaatctcc ttcaccgaat ctgtggatgc ggcgatctcc
      661 tcggtgcttg ccgccagatt ggtcatccgc tcatccactt cccggataat ctctgtcaga
      721 tgatccgact cctccaacag ctttccgatc gcctgactga tctcattctt gttatcgttg
      781 ctgtcgtttg cggttgctct gctgttctcc gagagattct tgatctcctc cgccaccacc
      841 gcaaatcctc tgcctgccgc accggcacgg gcagcctcga tggaagcgtt cagtgagagc
      901 agattggtct ggttggcaat cttcgtgatg tcgttgttgt tgttctcaag cttatccaaa
      961 agctccgtga tcgcctcaaa cgactgcttc atctcctgac agaatccggt cacgccggtc
     1021 atcgcatccg taatcgccgt gctctcgctc gcattgctgc tattcccggc agccatctgc
     1081 ccgatggcgg aatccagttc ctgaaaatcg gaattcagtc cctcgataat tcctgcaatc
     1141 agatcgcttc tgcgctggat ctccacattc gcctgctggc tttcctccgc agccgcctgt
     1201 gccgcacgcc ctctttcctc cgcctcgttc ttgataaaat gaatacagga attctcactg
     1261 ttgcacccgt tgtagatcgc tgtcgccata tccaggcagg tcttataacc gcaggcgctg
     1321 cagtcgatcg cacgatccat cgccgtctgc ttgttcagac ggctgtatac tgcttctaac
     1381 tcctgtccct ccggcacgcg gatctcattc cccttcgacc ggtcggtata ccggcgcgta
     1441 aaatccgccg gttcaagctg cgcaaactga cggttcagct gtgccagccg ctgcttcggc
     1501 gtcgccgatc tcgcccacgg tcccttgacc ttcttgctct tctcccggat cttatgcagc
     1561 tcgtagagca catcatcccc ggcagtcttc tccggctcaa cgcccgtacc gtagatgcat
     1621 cccatcgcac agttcagcgc atcgaccata aacggcagtt ccttgtgctc tgcgacacgc
     1681 ttgcgatagg catccaggaa ttcatagaca tggcgctctc cctcaatctg ccggataaat
     1741 acatcctctc cgcagaacca gtagacattc tccttca
//
LOCUS       DBMDQJ010000544         1774 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22380_length_1774_cov_1.404065, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000544 DBMDQJ010000000
VERSION     DBMDQJ010000544.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22380_length_1774_cov_1.404065"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="ACZO9R_10125"
     CDS             complement(<1..486)
                     /locus_tag="ACZO9R_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR0913452.1"
                     /translation="MPDIHFPRNSVSGNSLKNSLITGTLLLTLAGVLTRIIGFFYRIF
                     LSRLIGAEGLGIYQLLSPVMALGFAVTAAGIQTAISRFVSSELAQNNPAGARLYFRIG
                     LLLSLFLSAATGFVIWKYADFIGVSMLGDVRCIPLLKIISLSFVPCCIHACINGCYYG
                     QK"
     gene            540..1706
                     /locus_tag="ACZO9R_10130"
     CDS             540..1706
                     /locus_tag="ACZO9R_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR0913453.1"
                     /translation="MCDQEYKAGVVYLDNAATTRMAEEAEAAMQPYLVEKFGNASTAY
                     GYGEEAQAAIAHAREVIAASLDARPSEIYFTSGGSEADNWAVKGVAEAYGKYGRHIIT
                     SRIEHHAVLNSCHYLEEQGYEVTYLDVDQNGMISLEQLEQAVREDTILISVMFGNNEV
                     GTIEPVMQIGRFARDHQILFHTDAVQAYAQIPISMQQYHIDLLSASAHKFHGPKGVGF
                     LYVRDGVTMPSFIHGGSQERGKRAGTENVPGIVGMGKAVELAFCNMRRRIRREMMLRN
                     YLIEKIMHEIKGVRLNGHPTNRLPGNVNVSFSGVDGASLLILLYEDGICASGGSACNT
                     GEERVSYVIGALGVPDEYAPGTVRMTLSGETTREEIDRAVEALKQNLRKLRDEK"
BASE COUNT          473 a          383 c          559 g          359 t
ORIGIN      
        1 tttctgtcca tagtagcagc cgttgatgca ggcgtggata cagcaaggga caaaggagag
       61 ggagatgatc ttaaggagcg ggatgcaccg cacatccccg agcatggaaa caccgataaa
      121 gtctgcgtat ttccagatta caaagccggt cgcggcagat aaaaacagcg acagcagcag
      181 gccgatccga aaatagagcc tcgcacccgc gggattgttc tgcgcaagct cggaggagac
      241 gaagcgtgag atcgcagtct gaattccggc ggcggtgacg gcgaaaccga gcgccatcac
      301 cggggacagc agctggtaga tcccaagacc ttctgcacca atcagccttg agaggaatat
      361 tctatagaag aagccgataa tccgtgtgag cacaccggcg agtgtgagca gcagtgtgcc
      421 ggtgatcagg gaatttttca gagaatttcc agaaacggaa ttcctgggaa aatgaatgtc
      481 tggcaagtgt gtctccggga attggaatct ttttattata tgtaaggtgt gggggaaaaa
      541 tgtgcgatca ggaatataaa gcaggtgtgg tctatctgga taatgctgca acgacgcgga
      601 tggcggagga ggccgaagcc gctatgcagc cttatctggt ggaaaagttt ggcaatgcgt
      661 caacggctta cggttacggg gaggaagcgc aggcggcgat tgcccatgcg agagaggtga
      721 ttgcagcatc actggatgcc agaccgtcgg aaatctattt tacatcgggc ggatcggagg
      781 cggacaactg ggcagttaag ggggtggcgg aagcgtatgg aaaatacggt cgtcatatca
      841 ttaccagcag gattgaacat cacgcggtcc taaactcctg ccattatctc gaggagcagg
      901 gctacgaggt gacttatctg gacgtcgatc aaaacggaat gatctcgctg gaacagttag
      961 agcaagctgt ccgggaggat acgatcctga tctcggtcat gtttggaaac aatgaggtcg
     1021 gaacgatcga accggtcatg cagatcggaa ggttcgcaag agaccatcag attttgtttc
     1081 atacggatgc ggtgcaggca tatgcccaga ttccgatctc catgcagcag taccacatcg
     1141 atctgcttag cgcgagtgca cacaaatttc acggaccgaa aggtgtggga tttttgtatg
     1201 taagagacgg ggttacgatg ccttccttta tccacggcgg cagccaggag cgtggaaaac
     1261 gcgcgggaac ggagaatgtg ccggggatcg tcgggatggg gaaagcggtg gaactggcat
     1321 tctgtaatat gcgcagaaga atccgcaggg agatgatgct gcgcaattat ctgattgaaa
     1381 agatcatgca tgagatcaag ggggtccgcc tgaacggaca tcccacgaac cgcctgccgg
     1441 ggaatgtgaa tgtgagcttt tccggcgtgg acggtgcatc gcttctcatt ctgctgtatg
     1501 aggacggaat ctgcgcctcg ggcggctccg cctgcaacac cggggaggag cgggtctcct
     1561 atgtaatcgg ggcgttgggt gtgccggacg aatacgcacc ggggaccgtg cgtatgacgt
     1621 tgtcgggaga gacgacaagg gaagagatcg accgcgccgt ggaggcgctc aagcagaatc
     1681 ttaggaaact gagagatgaa aaatagtttt ctctggggaa aaaatatgtg aaaatattac
     1741 aaaaacgaaa cgtacctttg cgggaaatga gaaa
//
LOCUS       DBMDQJ010000545         1772 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22420_length_1772_cov_1.399761, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000545 DBMDQJ010000000
VERSION     DBMDQJ010000545.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22420_length_1772_cov_1.399761"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..114)
                     /locus_tag="ACZO9R_10135"
     CDS             complement(<1..114)
                     /locus_tag="ACZO9R_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HIR0913454.1"
                     /translation="MYELLKQEGRAKRGVFHTVHGDIQTPVFMNVGTVAAIK"
     gene            complement(161..1486)
                     /gene="aroA"
                     /locus_tag="ACZO9R_10140"
     CDS             complement(161..1486)
                     /gene="aroA"
                     /locus_tag="ACZO9R_10140"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR0913455.1"
                     /translation="MIEKYLVKKITEPVDWKVTVPGSKSMTNRALLMAVLSAGTVRVE
                     GVLFSDDSRHFLACLVALGFTVEIEEEKKCVTVTGCGGRIPRGEAVIDVGSAGTAARF
                     LAAMLGFSDGCYEIRCSAQMKRRPMAELFDLLSGAGAKITFLEEEGHLPVRITGCRYA
                     RADKTAGETPRELSLDITKSTQFLSALLLIAPMVPEGMKIHITSEKTDGSYIRITRKM
                     MQEFGVSVAFDGRDYEVPCRSSYRKETYMVEPDMSAACYFYAAAAVTGGRALVRHVHM
                     DNTQGDLRFLEVLEHMGCTADDTPEGILVTGPADGALRGITVNMNDFSDQALTLAAIA
                     PFADAPVRIEGIGHIRGQECDRIHAIQTNLTALGIRCEEEETAATIYPGSPHGGSVET
                     YDDHRVAMAFSLVGLRTDGIVIENPACCRKTFENYFTVLSELTGKKMEA"
     gene            1611..>1772
                     /locus_tag="ACZO9R_10145"
     CDS             1611..>1772
                     /locus_tag="ACZO9R_10145"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006856981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR0913456.1"
                     /translation="MAGSTLGTLFKITTWGESHGKGVGVVIDGCPAELSLCEADIQKF
                     LDRRKPGQSR"
BASE COUNT          365 a          568 c          414 g          425 t
ORIGIN      
        1 cttgatggca gccaccgtac ccacattcat aaataccggt gtctggatat ctccgtgcac
       61 cgtatggaaa acgcctctct tcgcgcgtcc ttcctgtttt aataattcat acatctgttt
      121 ttcgtctcct ttttattact catgcaacac cggcgctgct tcacgcctcc atcttcttcc
      181 cggtcagttc gcttaacacg gtaaaataat tttcaaacgt cttgcggcag cacgccgggt
      241 tctcaatgac aatcccgtcg gttctaagtc cgaccaggga aaatgccatc gccacgcggt
      301 gatcgtcgta tgtctccacg cttccgccgt gggggctgcc gggatagatc gtggcagccg
      361 tctcttcctc ctcacaccgg atgccaagcg ctgtcaggtt cgtctgtatc gcgtgaatcc
      421 ggtcgcattc ctgcccacgg atatgtccga ttccctctat gcgcaccggc gcgtcggcaa
      481 acggcgcgat cgccgccagg gtcagcgcct gatcggaaaa atcgttcata ttgactgtga
      541 tgccccgtag cgcaccgtcc gccggtcccg ttaccaggat gccctccggc gtatcgtccg
      601 cggtacatcc catgtgctcc aacacttcca ggaaacgcag atctccctgt gtgttgtcca
      661 tatgcacatg ccgcacaagc gcccgtccgc cggtcacagc cgcagccgca taaaaatagc
      721 aggcagccga catatccggt tccaccatat aagtctcttt ccggtaggaa cttctgcacg
      781 gcacctcgta gtctcttccg tcaaaagcga cactcacgcc gaattcctgc atcatcttcc
      841 gcgtgatgcg gatgtatgag ccgtccgtct tctcactggt gatgtggatc ttcatccctt
      901 ccggaaccat cggcgcaatc aacagcagcg cgcttaaaaa ctgtgtgctc ttcgtaatgt
      961 caagagagag ctcccgcggc gtctcccctg ctgtcttatc cgcccgggca tatctgcatc
     1021 ctgtgatgcg caccggaaga tgcccttcct cctcgaggaa tgtaatcttc gctcccgcac
     1081 cggaaagcag atcgaacagt tccgccatcg gccgccgctt catctgcgct gagcagcgga
     1141 tctcatagca cccgtccgaa aatccgagca tcgccgcaag aaaacgcgcc gctgtcccag
     1201 cgcttccgac gtcgattacc gcctccccgc ggggaatcct gccgccgcag cccgtcacgg
     1261 tcacgcactt tttttcttcc tctatctcca ctgtaaagcc gagcgccaca agacacgcca
     1321 gaaagtgtct ggaatcatcg gaaaacagga ctccttccac gcgcaccgtc cctgcggaca
     1381 gcactgccat aagaagcgcg cggttcgtca tgctcttcga tcccggcacg gttaccttcc
     1441 agtccaccgg ctctgttatc tttttcacaa ggtatttctc tatcatgaaa tattcttctc
     1501 ctcgcctgct ggtacttctg ctccgtcaaa tcataacata tttctgttgc ggcgcaaagt
     1561 caaatctttt ataatcgaga aagatcatta tgtaagcagg agggaatcac atggcagggt
     1621 ctactttggg aactcttttt aaaatcacaa cctgggggga atctcacgga aagggcgtcg
     1681 gcgtcgtcat cgacggctgt ccggcggaac tttctctctg tgaagcggat atccagaagt
     1741 ttctcgaccg gcgcaagccc ggtcagtcca ga
//
LOCUS       DBMDQJ010000546         1770 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22460_length_1770_cov_0.897543, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000546 DBMDQJ010000000
VERSION     DBMDQJ010000546.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22460_length_1770_cov_0.897543"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(156..1349)
                     /gene="coaBC"
                     /locus_tag="ACZO9R_10150"
     CDS             complement(156..1349)
                     /gene="coaBC"
                     /locus_tag="ACZO9R_10150"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR0913457.1"
                     /translation="MLTGKTIILGVTGGIAAYKAANLASLLKKQHADVHVIMTKNALE
                     FITPLTFETLTVNKCLTDTFDRNFKFEVEHIELAKKADLAIIAPATANVCAKLAHGLA
                     DDMLTTTLLACQCPRIIAPAMNTRMYENPITQDNLRLLEHYGFTIIEPASGLLACGDS
                     GKGKFPDEGLILEYILRAIAYPKDLAGKKILVTAGPTQEAVDPVRYLTNHSTGKMGYA
                     LARMAMLRGADVTLVTGETSLTPPPFVDTIRIKSAHDLFEAVTSRSEEQDIIIKAAAV
                     ADYRPAVVSDEKVKKSDGDLSLALERTEDTLSYLGAHKRPGQLLCGFAMETSQLEERA
                     RGKLERKNLDLIAANNLKVAGAGFGTDTNVITLIDKQQTRHLEQMSKEAAAMEILTVL
                     RDMMK"
BASE COUNT          399 a          421 c          474 g          476 t
ORIGIN      
        1 tgaaattatc ttgatcatag gagtatggcg gattcgtttt aaatagccac attctaaaaa
       61 taacacttat gatatattcc atattcaatg aaagaggagg catccgtcca tcgacctcgg
      121 ttgcttcctc tttctctatc tatttcaatc aaaatctatt tcatcatatc gcgtaaaacc
      181 gtcaatatct ccatcgctgc cgcttccttg ctcatctgct ccaggtggcg ggtttgctgc
      241 ttatcgatca gcgtgatgac gttcgtatcg gtgccaaatc cggcgccggc tacctttaag
      301 ttgttggcag cgatcagatc cagatttttg cgttccagct tgccgcgtgc gcgttcctcg
      361 agctgggaag tctccatcgc gaagccgcag agcagctgtc ccggacgttt gtgtgcgccg
      421 aggtagctta aagtatcctc agtgcgctcc agcgccaggc tcagatcgcc gtcggatttc
      481 ttgacctttt catcagaaac gactgccgga cggtagtcag cgacggctgc cgccttgatg
      541 ataatgtcct gttcctcact ccgggaggtg actgcctcaa agaggtcgtg tgcgcttttg
      601 atgcggatag tatcgacaaa cggaggcggg gtcagcgaag tttcgccggt gacgagtgtc
      661 acgtcggcac cgcgcagcat cgccatccgt gccagcgcat agcccatttt tccggtcgaa
      721 tggttcgtga ggtagcgcac cggatcgacc gcttcctggg tcggaccggc ggtcacaagg
      781 atcttctttc cagcgagatc cttcgggtag gcgatcgcgc ggagaatata ttccaggatc
      841 agcccctcat ccgggaattt gccctttccg gaatcgccgc aggcgagcag accgcttgcc
      901 ggttcgatga tggtaaagcc gtaatgttct aacaggcgga gattatcttg ggtaatagga
      961 ttctcataca tccgtgtgtt cattgccggt gcaatgatgc gcgggcactg gcaggctaag
     1021 agcgtggtcg tcagcatatc gtcggcaagt ccgtgtgcca gcttggcgca gacattggcg
     1081 gtcgccgggg cgatgatcgc gagatcagct tttttcgcca gctcgatgtg ttccacttca
     1141 aatttaaaat tacggtcaaa ggtgtcggtc aggcacttat tgaccgtcag ggtctcaaag
     1201 gtcagcggtg tgataaattc cagcgcattt ttggtcataa tcacgtgtac atccgcgtgc
     1261 tgtttcttca aaaggctggc aaggttcgcc gccttgtatg cggcgattcc gccggttact
     1321 cccaaaataa ttgtttttcc ggttaacata atacatatcc tttcggtttg cactctggtt
     1381 ttctgggtca ttttcgaaca gcatagcatc tggaggaaaa attgtaaacc ttttttgact
     1441 tccgttctct gttggggctt tggtaagcgc acataacagg agaggaaaga aacatgtatt
     1501 ttgtgaaaag acaataaaaa acagcacata ttctggtaga attgttggtt gtatgttgaa
     1561 aagtgatgtg atatagtgtg gaggtgtctg ctggctgacc tggttttcac tggatgtgaa
     1621 cagggacagg cggcagcagt tgtattgtat cccatagaaa acaggcaccg aacgtgctgg
     1681 cagcaaatct gcggaaagcg gcagaagaaa gaaacttctg ccggaatgtg cgtatctgca
     1741 tacggatctg tatctgagat ggagaatcat
//
LOCUS       DBMDQJ010000547         1758 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22646_length_1758_cov_1.584792, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000547 DBMDQJ010000000
VERSION     DBMDQJ010000547.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22646_length_1758_cov_1.584792"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>712)
                     /locus_tag="ACZO9R_10155"
     CDS             complement(<1..>712)
                     /locus_tag="ACZO9R_10155"
                     /inference="COORDINATES: protein motif:HMM:NF016119.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HIR0913458.1"
                     /translation="RSRKILGAVCVTAAFLCFGWYFWSDYTNHRGSQANEYLKMLKEN
                     PTEAAEMVDNDTFFSEDAPELAAGLDQTERENEPPPPVLPEYEAIVAQHSDFAGWITI
                     DGTKIDYPVMLTPNDGDYYLKRNVNGEDDINGTLFMDPRTDLVQRSTNIIIYGHNMKS
                     GAMFGSLKKYLDEDYWREHAQIRFDTIYEKGTYEVFAVCLAKVQYRNSQEFRYYDFIQ
                     ADSEEAFNDYLDHIIQLSV"
     assembly_gap    713..722
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<723..1007)
                     /locus_tag="ACZO9R_10160"
     CDS             complement(<723..1007)
                     /locus_tag="ACZO9R_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913459.1"
                     /translation="MAAYKIGNFTFDTEEEYARGLEDAKKIEKIQSTVDLNEPETALR
                     LYWLIRTGKIKFGSKVGKKFFLDIADAVAKSAAKNIAQAQAPEQQAGEEPR"
     gene            complement(1038..>1758)
                     /locus_tag="ACZO9R_10165"
     CDS             complement(1038..>1758)
                     /locus_tag="ACZO9R_10165"
                     /inference="COORDINATES: protein motif:HMM:NF012271.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR0913460.1"
                     /translation="GLEVKGVSSTYTYTGKAITPEPEVWADGVQLEKDKDYTVSYEDN
                     INAGVAKLVITASGTHYAGTKELSFQILRKSIHLCDIGSIQTQVYTGSDIKPTVTVED
                     DGKALELLSDYTLMYSNNRKAGTGVAAVAGKGNYTATKNLTFDIRPCDAGAAVVTGAS
                     ADSLSISWTGDGAVTGYEVYRAGADGKWQQVTRTRDAFYTDKKLAAETTYSYKVRSYL
                     VADGETYYGEFTGAVTGTTTK"
BASE COUNT          356 a          496 c          383 g          513 t
ORIGIN      
        1 acaccgagag ttgtatgata tgatcgaggt agtcgttaaa cgcctcctcg gaatccgcct
       61 gtataaaatc ataataacga aattcctgtg aatttcggta ctgcaccttt gccagacaga
      121 cggcaaacac ttcataggtg cctttctcat aaattgtatc aaatctgatc tgcgcgtgct
      181 ccctccagta atcttcatcc agatacttct tgagacttcc gaacatcgca ccgcttttca
      241 tgttgtgtcc gtagataata atattggtgc tgcgctgcac cagatccgtt ctcggatcca
      301 taaacaaggt tccgttgata tcgtcctccc cgtttacatt gcgcttcaga taataatccc
      361 cgtcattcgg ggtaagcatg accggatagt caatcttcgt cccgtcaatg gtgatccatc
      421 cggcaaaatc actgtgctgc gccacgatgg cttcatattc cggcagaacc ggaggaggcg
      481 gttcgttctc ccgctccgtc tggtctaaac ccgccgcaag ttccggtgcg tcctccgaaa
      541 aaaaggtgtc attgtccacc atctctgcag cttctgtcgg attttctttt aacatcttca
      601 aatactcatt cgcctgactg ccgcggtgat tcgtgtaatc tgaccagaaa taccagccaa
      661 aacatagaaa tgcggccgtc acacacaccg caccgagaat ctttctgctg cgnnnnnnnn
      721 nnccggggtt cctcccccgc ctgttgttcc ggtgcctgtg cctgcgctat attttttgcc
      781 gcggattttg ccacggcatc tgcaatatca agaaaaaatt tcttgccgac tttgctgccg
      841 aacttgatct tccctgttcg gatcagccaa tagaggcgta gagcggtttc cggttcattg
      901 agatcgacgg tgctctgaat cttttcgatt ttcttcgcat cttcgagtcc tctggcatat
      961 tcctcttctg tgtcaaatgt aaaattccct atcttatatg ccgccatgat cggaaccgct
     1021 ctccctcctc tctgtcttta ttttgtggtt gttccggtaa ctgctccggt aaactcaccg
     1081 tagtaagtct cgccgtccgc caccaggtag cttcttactt tataagagta tgttgtctct
     1141 gccgcaagct ttttatcggt atagaaagcg tctctggtac gcgttacctg ctgccacttt
     1201 ccatctgcgc cggcacggta tacttcgtat cccgtcacgg caccgtcgcc cgtccagctg
     1261 atggacagac tgtctgcgga tgcgcctgtc acaaccgccg cgcccgcatc gcacggtctg
     1321 atgtcaaacg tcaggttctt tgttgccgtg taattgcctt ttcctgcaac tgccgccaca
     1381 ccggtacctg ccttgcggtt gttggaatac atcagtgtgt agtcgcttaa aagttccagg
     1441 gctttgccgt catcttccac cgtcacggtc ggcttgatgt cagatccggt atatacctgt
     1501 gtctgaatcg aaccgatgtc gcacagatgt atgctcttgc gcaggatctg gaagctcagt
     1561 tccttagtac ctgcataatg ggttccgctt gctgtgatta ccagcttcgc tacacccgcg
     1621 ttgatgttgt cttcatagga taccgtgtaa tccttatctt tttccagctg cacgccgtct
     1681 gcccatactt ccggctccgg tgtgattgct tttccggtat aagtgtaagt agaagaaaca
     1741 cccttcactt ccagtccg
//
LOCUS       DBMDQJ010000548         1754 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22709_length_1754_cov_1.526316, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000548 DBMDQJ010000000
VERSION     DBMDQJ010000548.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22709_length_1754_cov_1.526316"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="ACZO9R_10170"
     CDS             <1..362
                     /locus_tag="ACZO9R_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913461.1"
                     /translation="ANLNTSISTSQDALNQSMENVDSTYEVFDQITTAAGGADTVHQQ
                     ITTAMSAAEQKLDEVSQSFSLEERQFDAVMEHINHANDLGTTKSSLFEDMTNLLTQIA
                     PLTEELEKNTIVLDNSK"
     regulatory      401..502
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            548..1231
                     /gene="thiT"
                     /locus_tag="ACZO9R_10175"
     CDS             548..1231
                     /gene="thiT"
                     /locus_tag="ACZO9R_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015559391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015234 - thiamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HIR0913462.1"
                     /translation="MNNFFATPEGGWGDGSVHLTTVGIAIVIAVIALLLIVAAIVRHK
                     NATHKSALTTKQLVYSAVAIALAVVCSMIKLFDMPMGGSVTLLSMLFIVLIAYWYGPY
                     VGIMTAVAYGLVQFVLEPIFYTIPQMLLDYPLAFGALGLAGFFNKKKWGLQIGYIAGV
                     IGRFVFATISGVVFFAAYAPEGMSPLAYSAGYQASYLVPEAIITLIIICIPPVSHALA
                     EVRKRAVEA"
BASE COUNT          438 a          453 c          423 g          440 t
ORIGIN      
        1 ccgccaacct gaataccagt atttccacct cgcaggacgc cctgaaccag agtatggaaa
       61 atgttgattc cacgtatgaa gtgtttgatc agatcacaac cgctgccggc ggtgcggaca
      121 ccgtacacca gcagattaca accgccatgt ccgccgcaga gcagaagctg gatgaagtca
      181 gccagtcttt cagccttgag gaacggcagt ttgacgctgt tatggagcat atcaatcacg
      241 ccaatgatct cgggaccacc aagagttccc tcttcgagga catgaccaat ctgctcacac
      301 agattgcgcc gctcacagaa gaactggaga agaacacaat cgtacttgac aattccaaat
      361 agaatcgtta tagtaaagtt cgttataata atatcgaata tgaaaacgct aggggtgcag
      421 attctgctga gagatgatcg ttcatcgacc cttattactt gaaccggata ataccggcgt
      481 aaggaagctt tttgcaataa cgcagacaaa tcctcctggc tacatattca ccaaggagga
      541 tttttttatg aataactttt ttgctacacc ggaaggcggc tggggtgacg gttccgtcca
      601 tctgactacc gtcggtattg caatcgtcat cgcagtcatc gcccttcttc tgattgttgc
      661 cgctatcgtg cgccacaaga acgccacgca caagtccgca ctgaccacaa agcagctcgt
      721 ctactctgct gttgcgatcg cgctggccgt tgtatgctcg atgatcaagc tgttcgacat
      781 gccaatgggt ggttccgtca ctctgctgtc catgcttttc atcgttctga tcgcttactg
      841 gtatggtccg tatgtcggca ttatgaccgc agtggcttac ggcctggtgc agttcgtact
      901 ggagccgatt ttctacacaa tcccgcagat gctcttagac tacccgctgg cattcggtgc
      961 tctgggtctt gccggcttct tcaataagaa aaagtgggga cttcagatcg gctatatcgc
     1021 cggcgtcatc ggacgttttg tatttgcaac gatctccggc gttgtatttt tcgcagcata
     1081 cgcaccggaa ggcatgagcc cgctggcata ttctgccggc tatcaggcat cctaccttgt
     1141 tccggaggca atcatcacct tgattattat ctgcattccg ccggtatcac atgcacttgc
     1201 ggaagtccgc aaacgcgccg tagaagcgta ggcagcgcta taaaaaggac tgccgccccc
     1261 gcgctttgca ctttaaaacg ggatatgttt tattataaga atattggaga atatgaagtt
     1321 ccagaatgca accagaggcg gcaattcttt gaagcaaaat ccgaggattt cttccgtgcc
     1381 gcgtcgaaca tcgagttctg tgtctttcca cgtttggaca gtccccgggg cgtgtccgac
     1441 aaaacacaga caatgagcgg actttctggt gcccctagtg gcaccgcttc acagacaata
     1501 taaaaccgcg cgaaccgaaa ctcgttatca tctgctattg aaaacaaggc agatgataac
     1561 tcagacatcg gcttcgcgcg gttatgttgt gttcagtggt gccaacaagg ggcacacaca
     1621 aaagtcctta aattgtctgc ggtttgcgga cacgccacgt ggaatgttcc aaacttggaa
     1681 aaacacagat gatcaaggat gttcgacgcg gcatggttag aaataccgga ttttgcgctt
     1741 agaattgccg cctc
//
LOCUS       DBMDQJ010000549         1752 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22738_length_1752_cov_1.421563, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000549 DBMDQJ010000000
VERSION     DBMDQJ010000549.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22738_length_1752_cov_1.421563"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1752)
                     /locus_tag="ACZO9R_10180"
     CDS             complement(<1..>1752)
                     /locus_tag="ACZO9R_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013986.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HIR0913463.1"
                     /translation="IRREVADEEQKRKALPRYDYEKEALHRTRPLRGGEKLWLGIDVG
                     STSTNLVLTGEDGAVIDDLYLRTRGNPLGAVQQGMAQLKRKYGEALTWEGIGVTGSGR
                     YYIAEKTGAGTVLDEITAQARAAAHLCPEVDTVFEIGGQDSKYISIRDGQVVDFEMNK
                     VCAAGTGSFVEEQANRMEVSLEEIGNSALKANVPIDLGERCTVLMESKIAAELAAGTD
                     KEDICAGLCRSIVRNYLNRVVGNKRKGEHICLQGGVVHNEGIVAAFYEVFGERLHITP
                     FYDVTGAYGAALAAKEQGGTSQKESIRNEENYRKSQKWFLAGYDGTLLPGKKTVGIPR
                     ALMIYKFFPMAYQYFKTLGFNVLLSPETDDKIIALGQEMAAEETCYPVKLLHGHMEWL
                     ARKKVDYIFIPCIHTIRHETSGVAHNYGCVYMQTAPRIVASVLGLSERGVELICPVLD
                     LDMGKPQLAGAMIKAGQELGCDKARCAAALAKGAAAMVRCEKNSEKLGAQILDELKPE
                     DKVLVLITRNYGVSDPVLNMGIPGEMLKRGCRVLTLSHLKGHDVDLSGHYPNLYWPFA
                     QHILSGAKIIKEHPNLYA"
BASE COUNT          354 a          585 c          384 g          429 t
ORIGIN      
        1 ggcatacaga ttcggatgct ccttaatgat cttcgctccc gacagaatgt gctgcgcaaa
       61 tggccagtac aaatttggat aatgaccgga caaatccaca tcatgtcctt tcagatggga
      121 aagtgtcaac actctgcacc ctctctttaa catttccccg ggaattccca tatttaaaac
      181 cgggtctgac acgccgtagt ttcttgtaat cagaacgagc accttgtcct ccggtttcaa
      241 ctcgtccagg atctgcgcgc ccagtttttc agaattcttc tcacagcgca ccatcgccgc
      301 cgcgcccttc gcgagcgccg ccgcacaccg tgccttgtcg catcccagtt cctgacccgc
      361 tttgatcatc gctcccgcga gctgcggttt tcccatatcc agatccagaa ccgggcagat
      421 cagctccacg ccgcgttccg aaagccccag cacacttgcg acaatccgcg gcgctgtctg
      481 catatagaca cagccgtaat tgtgcgcaac gccggaagtc tcatgccgga tcgtatggat
      541 acacgggata aaaatgtagt ccaccttttt tctcgcaagc cattccatgt gcccgtgcag
      601 taattttacc ggataacagg tctcctccgc cgccatctcc tgtccgagcg caatgatctt
      661 atcgtccgtc tccggcgaca gcaagacatt aaagccgaga gtcttaaaat actggtacgc
      721 catcgggaaa aatttataaa tcatgagcgc acgcgggatt cccacagtct ttttccccgg
      781 cagcagcgtt ccgtcatagc ctgccagaaa ccatttctgg ctcttccggt aattctcctc
      841 attccggatg ctctcctttt gggatgtgcc accctgctct tttgccgcga gcgccgctcc
      901 gtatgcccct gtcacatcgt aaaacggcgt gatgtgaagc cgttctccaa acacttcata
      961 gaaagccgca acgattccct cgttatgcac gactccgccc tgaaggcaga tatgttctcc
     1021 cttgcgctta ttgccgacta ccctgtttaa atagtttctg acaatcgagc ggcacagccc
     1081 tgcacagata tcctccttgt ccgtgcctgc cgcaagctcc gctgcaatct tggattccat
     1141 caacacggtg cagcgctctc ccagatcgat cggcacattt gctttcaagg cactgtttcc
     1201 gatctcctcc agcgatactt ccatgcggtt cgcctgttcc tcgacgaagg agcccgtacc
     1261 cgccgcacac actttgttca tctcgaaatc gacgacctga ccgtcccgga tactgatgta
     1321 tttggagtcc tgaccgccga tctcaaacac ggtatccacc tccgggcaca gatgggcagc
     1381 cgcgcgcgcc tgcgccgtga tctcatccaa caccgtgcct gcaccggtct tctccgcgat
     1441 atagtaccgc cccgatcccg tgacaccaat gccttcccac gtgagtgctt ccccgtattt
     1501 ccgctttaac tgtgccattc cctgctgcac cgcgccgaga ggattgccgc gcgttctaag
     1561 atacagatcg tcaatgacgg ctccgtcctc ccccgtcagc acaaggtttg tgctcgtgga
     1621 tcccacgtca atcccgagcc agagcttttc cccgccacgt aacggtctgg tccggtgaag
     1681 tgcctccttc tcgtagtcat atcgcggcag cgcctttctt ttttgctcct catccgccac
     1741 ctccctgcgg at
//
LOCUS       DBMDQJ010000550         1740 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22921_length_1740_cov_1.313606, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000550 DBMDQJ010000000
VERSION     DBMDQJ010000550.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1740)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22921_length_1740_cov_1.313606"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(243..>1740)
                     /locus_tag="ACZO9R_10185"
     CDS             complement(243..>1740)
                     /locus_tag="ACZO9R_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR0913464.1"
                     /translation="TSGSDRNYIYKDKSERYRYMNTFYIFAVVILWVLFGVYLLAKLA
                     SKSIAVGTAVGNVVLIILFAVVELAFYLRNKSDVRLKRVVAIGVGVEFALIGMQTDAE
                     FIYFALIAILTLQIPYYDMKRYKQTCIAFSALFTLVEVIRVIKGIGLQDVDSLCRVLC
                     VYLLFFIMYRVGSIAKTFSDHALGSAASEGEKQKVMLEGILDISKTVRDESERSTSLV
                     DELVHTSETVAQSMQEISSATNTTAKNIEEQNNMTQSIQSAIDETGERSRKMVDVATD
                     SNENIQENLQVMEELKNQSEQIAATNHEVTESMTRLQNKTKEVEEIAGMILNISNQTN
                     LLALNASIESARAGEAGRGFAVVAEQIRQLAEQTKKSTEEITTIINELNENARDVMTS
                     VESSVEATESQNEKIAVAAESFERLNENMSQLIGDINEIAGQITGLSDSNNRIVENIS
                     QLSAATEEVTASAEQVREISEQNRNYAEEVKGAIGTIRGRTDEMKQFT"
BASE COUNT          414 a          500 c          341 g          485 t
ORIGIN      
        1 gcgcggttat gttttgttca gaggtgtcca caagggacac acacaaaagt cctaaaattg
       61 tctgccggct tgcggacaca gtccgtggag tgttccaaac ttggaaagac acggatcatc
      121 gaagatggca agcgcggcat ggttagaaat accggatttt gcggtcagaa ttgccgccgt
      181 ccgttgtatc cggaacttcc agttatctgt tttttgcaat atcacggatc ccgcttttct
      241 ttttatgtaa actgcttcat ctcgtcagtt ctgcctcgga tcgttccgat ggcccccttt
      301 acttcctccg catagttccg gttctgctcg cttatctcac gcacctgctc ggcgcttgca
      361 gtcacttcct ctgtcgctgc agacagctgt gagatattct ccacaatacg gttgttggaa
      421 tccgacagtc ctgtgatctg tcccgcgatc tcattgatat ctccgataag ctgtgacata
      481 ttttcattca gacgttcaaa agactctgcc gcaaccgcaa tcttctcatt ctggctctct
      541 gtcgcttcca ccgaagactc caccgaagtc ataacgtcgc gtgcattttc attcagctcg
      601 ttgatgatcg tggtaatctc ctccgtggac ttcttcgtct gctcggcaag ctgtctgatc
      661 tgttcagcaa ccacggcaaa tccgcgtcct gcctcaccgg ctctcgcact ctcaatggat
      721 gcgttgagtg ccagcaggtt cgtctggttg gagatattga ggatcatgcc tgcgatctcc
      781 tcgacctcct tggtcttatt ctgcaggcgt gtcatggact ccgtcacttc atgattcgtc
      841 gccgcaatct gctcggactg gttcttcaat tcttccatta cctgcagatt ctcctgaata
      901 ttctcattgg aatccgtcgc cacatccacc atctttctgg agcgctcgcc cgtctcgtca
      961 atagccgact ggatactctg tgtcatatta ttctgctctt cgatattctt cgccgtcgtg
     1021 ttggttgcgg aagaaatctc ctgcatgctc tgcgcaactg tctccgaagt gtgcaccagt
     1081 tcatccacaa gactggtact cctctcagac tcatcccgca cagtctttga gatgtcaagg
     1141 attccttcca gcatcacctt ctgtttctca ccctcggacg ctgcagaacc cagtgcatga
     1201 tcgctgaatg tcttggctat gctgcccacc cggtacataa tgaaaaataa cagatacaca
     1261 cataataccc ggcacaagct gtccacatcc tgcaaaccaa tccccttgat tacacgaatg
     1321 acctccacca gtgtaaataa cgccgaaaaa gcgatacagg tctgtttata tctcttcata
     1381 tcataatacg gaatctgcaa tgtcagaatg gcaataagtg caaaatagat aaactctgca
     1441 tctgtctgca ttccaatcaa cgcaaattct acgccgacac cgatggcaac aaccctcttt
     1501 aatctcacat cagacttatt gcgcagataa aacgccagtt caaccaccgc aaataaaata
     1561 atcagcacaa catttccgac cgccgtaccg actgcaatgc tcttgctcgc aagcttcgcc
     1621 agcagataca ccccaaacag tacccataaa ataaccactg caaaaatata aaatgtattc
     1681 atatagcggt agcgttcact cttatcttta taaatgtaat tcctgtctga acccgatgtc
//
LOCUS       DBMDQJ010000551         1739 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_22936_length_1739_cov_1.355922, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000551 DBMDQJ010000000
VERSION     DBMDQJ010000551.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22936_length_1739_cov_1.355922"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="ACZO9R_10190"
     CDS             complement(<1..207)
                     /locus_tag="ACZO9R_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
                     /protein_id="HIR0913465.1"
                     /translation="MYAYIKGVLADVEEESVVVEAGGIGYRIYTTGQTFDYLPAVGEE
                     VKLYTYLNVREDAMILYGFLTKDDL"
     gene            complement(221..715)
                     /locus_tag="ACZO9R_10195"
     CDS             complement(221..715)
                     /locus_tag="ACZO9R_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HIR0913466.1"
                     /translation="MRDKVAYLGVFLALALICSYVESLIPFYFGIPGVKLGLTNIVVV
                     LMLYCVGTKEAFAISMLRIVLAGFLFGNLFSILYSLAGGMFSFLVMYLLKRTGKLGVL
                     PVSVAGGMSHNIGQIAVAAFVVENINIFYYLPVLFVAGIVTGLLIGVAAQEIILRIGG
                     RLKR"
     gene            complement(705..1091)
                     /locus_tag="ACZO9R_10200"
     CDS             complement(705..1091)
                     /locus_tag="ACZO9R_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HIR0913467.1"
                     /translation="MKKRISRNDVIFLAILLAVVTVFLVVYERTGTRADAGACVRVTV
                     DGSVYGTYALGEEQEIPIVQDGVTTNVLTIRDGRADMTEADCPDKLCVHQKAISKNHE
                     MIVCLPNKVVVEVTGSEENGFDSIAR"
     gene            complement(1088..>1739)
                     /locus_tag="ACZO9R_10205"
     CDS             complement(1088..>1739)
                     /locus_tag="ACZO9R_10205"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR0913468.1"
                     /translation="ERIRFMTSHPKDLSDELIEVMGRSKKICKHLHLPLQSGSSRILK
                     IMNRRYDKEHYLELVDKIRAAVPDIALTTDIIVGFPGETEEDFEETMDVVRKVRYDSA
                     FTFIYSKRTGTPAASMEEQVPEDVIKARFDRLLKEVQKISAEKAGALTGQTLPVLIEE
                     QNEQDASLVTGRLSNNSVVHLPGTADMIGKILDVKLTECRGFYYLGERADGNGQTV"
BASE COUNT          402 a          565 c          366 g          406 t
ORIGIN      
        1 cagatcatcc ttcgtcaaaa acccgtacag aatcatcgca tcttcgcgca cattcagata
       61 cgtatagagc ttgacttcct ctcccacagc cggaagatag tcaaacgtct gcccggtcgt
      121 atagatccgg tatccgattc cgcctgcctc cacaaccacg ctctcttctt ccacatccgc
      181 aaggacgcct tttatatatg cgtacacacg tatttcctcc ttatcgtttc agccgtccgc
      241 caatccttag tatgatctcc tgggctgcca caccgatcag cagtcccgta acgatgcctg
      301 ccacgaaaag caccggcaga tagtaaaaaa tgttgatatt ctccaccaca aacgccgcca
      361 cggcaatctg accaatgttg tgtgacattc cgcccgcgac gctgaccggc agcacaccga
      421 gctttccggt ccgtttcaaa agatacatca ccagaaagct gaacattccg ccggcaaggc
      481 tgtataagat actgaacaga ttgccgaaca gaaaacccgc cagtacaatc cggagcatcg
      541 agatggcaaa cgcctcctta gtgccgacac agtacagcat cagtaccacc acaatattgg
      601 tgagccccag ctttaccccc ggtattccga aataaaacgg gatcagggat tccacatagc
      661 tgcagattag ggcaagcgca agaaaaaccc cgagatacgc caccttatct cgcaatggag
      721 tcgaatccgt tttcttcact tcccgtcacc tccacaacca ctttgttcgg cagacagaca
      781 atcatctcat gattcttaga gattgccttc tgatggacac acagcttgtc cgggcagtcc
      841 gcctccgtca tgtccgccct gccatcgcgg attgtcagga cattcgttgt cacgccgtcc
      901 tgcacaatcg gtatctcctg ctcctccccg agcgcatagg tcccgtacac actgccgtcc
      961 acggtgacgc gcacacaggc acccgcatcc gcgcgcgttc ccgtccgctc atagaccaca
     1021 agaaataccg taaccacggc aagcagtatt gcaagaaaaa tcacatcgtt tctgctgatt
     1081 cgctttttca taccgtctga ccatttccgt ctgcccgctc gccaaggtaa taaaagcccc
     1141 ggcactcggt cagctttaca tccagaatct ttccgatcat gtccgctgtc cccggcagat
     1201 gtaccaccga gttattgctg agtcttcccg tcacaagcga agcatcctgc tcgttttgct
     1261 cttcaatcag caccggcagc gtctgtccgg tcagcgcccc cgctttttct gcggagatct
     1321 tctgcacttc cttaagcagg cggtcaaaac gcgccttgat cacatcctcc ggcacctgct
     1381 cttccatgga tgccgccggt gtcccagtac gtttggaata gataaacgtg aacgcgctgt
     1441 cataacgcac ttttcgcacc acgtccatcg tctcctcgaa gtcctcctcc gtctcccccg
     1501 ggaatcccac gataatatcc gtcgtcagcg caatgtccgg cactgccgcg cggatcttgt
     1561 ccaccagctc cagataatgt tccttatcat atctgcggtt catgatcttt aagatccggc
     1621 tgctcccgga ctgtaacggc aggtgcagat gtttgcagat ctttttggat cgtcccatca
     1681 cctcgatcag ctcgtccgag agatccttcg ggtgggaggt cataaaacgg atacgctct
//
LOCUS       DBMDQJ010000552         1730 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23090_length_1730_cov_1.122775, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000552 DBMDQJ010000000
VERSION     DBMDQJ010000552.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23090_length_1730_cov_1.122775"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            22..>308
                     /locus_tag="ACZO9R_10210"
     CDS             22..>308
                     /locus_tag="ACZO9R_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR0913469.1"
                     /translation="MDFGAIGIFTPFPELNTIMHKVWTDKSTISFSSICVSSYAYHFT
                     EILHAENPTAKEKMSKGHLYDLKSNVMDRLPDIFLSNPLDDTEYLTVLGRTG"
     assembly_gap    309..318
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <319..754
                     /locus_tag="ACZO9R_10215"
     CDS             <319..754
                     /locus_tag="ACZO9R_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR0913470.1"
                     /translation="RVYKYIRRKYIQTIDNTDLFKVFISAATGTGQFGETFAEPIVAE
                     PGLIATETFSSIGAFSTEQEANNCLKYIKAKFARALLGVLKKTQHLTVENWKYVPLQD
                     FTPASDIDWSRSIHEIDLQLYRKYGLDEKEIEFIESHVKEMA"
     gene            754..>1730
                     /locus_tag="ACZO9R_10220"
     CDS             754..>1730
                     /locus_tag="ACZO9R_10220"
                     /inference="COORDINATES: protein motif:HMM:NF016724.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HIR0913471.1"
                     /translation="MAAIQIKTASKVVPQCYAYTTPGVTAHDGWTKIGFTERDVETRI
                     NEQTHTVGVAHKTWWAMRAAYMTEPYGTFTDKDFHAYLKKLGISREAGTEWFRIEPNA
                     ARGNFIDFTQNHGVVSEDDVDAVIPYKLREEQAEAVQRTADYFRSRENAEFLWNAKPR
                     FGKTLSAYDLCMKLGAQNILIVTNRPAIANSWYQDYETFFGPQSGYLFVSNVDGIKDR
                     KFVYSREEYIAKLNENTKGCIEFVSLQDLKGSIYFGGRFDKLSELSAEKGLTWDVLIV
                     DEAHEGVDTYKTDTAFNHIRRKWTLHLSGTPFKALANDKFPESAIYNWT"
BASE COUNT          460 a          409 c          437 g          414 t
ORIGIN      
        1 ataacatatc gtgatgattc catggatttt ggtgccatcg gaatttttac gccttttcct
       61 gaattgaata caattatgca taaagtgtgg acggacaaga gcactatttc tttctcttca
      121 atatgcgtgt ccagctacgc ataccatttt actgagatcc tacacgcaga gaacccaacg
      181 gcaaaagaaa aaatgagcaa ggggcatttg tatgatttga aatcgaacgt catggacagg
      241 ctaccggata tctttctttc aaatccgctt gatgacaccg aatatcttac cgttttgggc
      301 cgaacaggnn nnnnnnnnac gagtttataa atatattcgc cgaaaatata ttcagacgat
      361 tgacaacacg gacttattta aggttttcat atcagccgct actggaacgg ggcagtttgg
      421 agaaaccttt gctgaaccga ttgtcgctga accgggattg attgctacag aaaccttctc
      481 cagcattggg gcattttcta ctgagcaaga agccaataat tgcttgaaat atatcaaggc
      541 caaatttgcc cgcgcccttt taggcgtgct gaaaaagacc caacacttaa ctgtggaaaa
      601 ctggaaatat gtcccccttc aggacttcac tcccgcctcc gacatcgact ggtctcgctc
      661 tattcatgag attgacttac agctttatcg caagtacggg ttggacgaaa aggagatcga
      721 gtttatagaa tctcatgtaa aggagatggc ataatggcag cgatccagat caaaacagcc
      781 tccaaggtcg ttccgcagtg ctatgcctac accactccgg gagtcacggc gcatgacggt
      841 tggacgaaga tcggctttac cgagcgggat gtggaaactc gtattaacga gcagacccac
      901 acggtgggtg tcgcgcacaa gacctggtgg gcaatgcgtg ccgcctatat gacagagccg
      961 tacgggacat ttacggataa ggacttccac gcttacctga agaagctcgg tatttcccgc
     1021 gaggccggga cggagtggtt ccggatcgag ccgaatgccg cccgcggtaa ttttatcgac
     1081 ttcacgcaga atcacggtgt ggtatcggag gatgatgtcg atgcagtgat tccgtacaag
     1141 ctccgcgagg aacaggcgga ggctgtgcag agaacggcgg attatttccg cagtcgtgag
     1201 aatgccgagt ttctttggaa cgcaaagcct cgctttggaa aaacgctgtc cgcctatgac
     1261 ctgtgcatga agctcggtgc acagaatatc ctcatcgtga cgaaccgtcc ggcgattgca
     1321 aattcctggt atcaggatta tgagaccttc ttcggccccc agtccggcta tctctttgtc
     1381 agtaatgtgg acggaatcaa ggacagaaag tttgtttaca gccgagagga atacatagca
     1441 aagctcaatg agaacacaaa gggctgcatt gaatttgtca gtctccagga tttgaagggc
     1501 tccatctatt tcggcggacg ctttgataag ctgtcggaac tcagcgcaga gaaagggctg
     1561 acatgggatg tcctcatcgt agatgaggcg cacgagggcg ttgataccta taagaccgac
     1621 accgccttta accatatccg ccgcaagtgg acgctgcatc tttccggcac accgtttaag
     1681 gcgctggcga atgacaagtt cccggagagc gccatctaca actggacgta
//
LOCUS       DBMDQJ010000553         1727 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23129_length_1727_cov_1.898524, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000553 DBMDQJ010000000
VERSION     DBMDQJ010000553.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23129_length_1727_cov_1.898524"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="ACZO9R_10225"
     CDS             complement(<1..411)
                     /locus_tag="ACZO9R_10225"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase domain-containing
                     protein"
                     /protein_id="HIR0913472.1"
                     /translation="MKSDNVKKGMQQAPHRSLFNALGFTEEEMNRPMVGIVSSYNEIV
                     PGHMNLDKIVNAVKLGVAEAGGVPVVFPAIAVCDGIAMGHIGMKYSLVTRDLIADSTE
                     CMALAHQFDALVMVPNCDKNVPGLLMAAARINVPT"
     gene            complement(437..1537)
                     /gene="leuB"
                     /locus_tag="ACZO9R_10230"
     CDS             complement(437..1537)
                     /gene="leuB"
                     /locus_tag="ACZO9R_10230"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR0913473.1"
                     /translation="MEYKVGVISGDGIGPEIVTEAKKVLDQIGKTYGHVFRYEEILMG
                     GCSIDATGEPLTDAAIASAKASDAVLMGSIGGNTSTSPWYQLPPEKRPEAGLLKLRKA
                     LNLFANLRPAYLYEELKAACPLRDDIIGDGFDMMIMRELTGGLYFGARKTEEVDGVLT
                     ATDTLTYNENEIRRIAKRGFDIAMKRRKKVTSVDKANVLDSSRLWRKIVEEVAQDYPE
                     VIYEHMLVDNCAMQLVKDPKQFDVILTENMFGDILSDEASMVTGSIGMLSSASLNDTK
                     FGLYEPSGGSAPDIAGKGIANPIATILSAAMMLRYSFDLDREADAVEQAVKQVLKDGY
                     RTIDIMPQEKQQEKVIQVGTAQMGDLICERIR"
BASE COUNT          368 a          541 c          357 g          461 t
ORIGIN      
        1 tgtgggtaca ttgatgcgcg ctgccgccat caaaagtccc gggacattct tatcgcagtt
       61 cggaaccatg acgagcgcgt caaactgatg cgcaagcgcc atacattccg tggaatcggc
      121 gatcagatca cgcgtcacca gcgaatattt cataccgata tgtcccatcg cgattccgtc
      181 gcagaccgcg attgccggaa ataccaccgg cacgccgcca gcctccgcaa cgccaagctt
      241 taccgcgttg acaatcttat ccaaattcat gtgaccggga acaatctcgt tgtaggagct
      301 cacaataccg accatcggcc tgttcatctc ctcctcggta aatcccagcg cgttaaacaa
      361 acttctatga ggtgcctgct gcatcccttt ttttacatta tcactcttca tctatcttaa
      421 ctccgtttct gtcattctat ctgatccgct cgcagatcag atctcccatc tgtgccgtgc
      481 cgacctggat taccttttcc tgctgcttct cctgcggcat gatgtcgatc gtccggtagc
      541 catcctttaa gacctgcttc accgcctgct ccactgcatc cgcctcccgg tccagatcaa
      601 agctataacg cagcatcatg gctgcactta agatcgttgc gatcggattc gcaattccct
      661 ttcctgcaat gtccggtgcc gaaccgccgc tcggctcgta gagtccgaac tttgtatcgt
      721 ttaggctggc gcttgagagc ataccgatcg aaccggtcac catgctcgcc tcatccgaca
      781 aaatatcgcc gaacatgttc tccgtcagaa tcacgtcgaa ctgcttcgga tcttttacca
      841 gctgcatcgc acagttgtcc acgagcatat gctcatagat cacttccgga taatcctgcg
      901 ccacctcctc gacgatcttt ctccagagtc tcgaggaatc cagcacgttt gccttgtcca
      961 cactcgtcac ctttttgcgc cgcttcatgg caatgtcaaa accgcgctta gcgatccgtc
     1021 tgatctcgtt ctcattgtag gtcaacgtgt ccgtcgctgt cagcacgccg tccacttcct
     1081 cggtcttgcg cgctccgaaa tacagtccgc cggtcagttc ccgcataatc atcatgtcaa
     1141 atccgtcccc gatgatgtcg tcccgaagcg ggcatgcggc ttttaattct tcatataaat
     1201 atgccggtct taagttggca aacaggttca gcgccttacg gagctttaaa agtcccgcct
     1261 ccggtctctt ctccggcggc agctgatacc acggcgaagt cgatgtgttg ccgccgatcg
     1321 atcccatcag caccgcatcg gaagccttcg cggaagcgat cgccgcatcc gtgagcggct
     1381 cacccgtcgc gtcaatggag cagcctccca tcagaatctc ttcatagcgg aacacatgtc
     1441 cgtaggtctt tccaatctga tccagaactt tctttgcctc tgttacgatc tccggtccga
     1501 tcccgtcgcc ggaaatcacg cctaccttat attccatctc tctaatccct cttttcttcc
     1561 tgccatgcag gaatccgtct tgtattgact ttagtacatt actaacagac agtatataaa
     1621 ttctataata atgtatctcg ggaaacaatg caacctctga atccattttt ctttttcata
     1681 ctccctataa ccgggtctta tgaaaaagga gcagcctgcc ttttgtg
//
LOCUS       DBMDQJ010000554         1723 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23195_length_1723_cov_1.129470, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000554 DBMDQJ010000000
VERSION     DBMDQJ010000554.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23195_length_1723_cov_1.129470"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            28..101
                     /locus_tag="ACZO9R_10235"
     tRNA            28..101
                     /locus_tag="ACZO9R_10235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:62..64,aa:Arg,seq:ccg)
     gene            complement(172..1488)
                     /locus_tag="ACZO9R_10240"
     CDS             complement(172..1488)
                     /locus_tag="ACZO9R_10240"
                     /inference="COORDINATES: protein motif:HMM:NF015531.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR0913474.1"
                     /translation="MKDAQPTKAELLAEINEEHRRYEGGSSDPTPYLRHYDDPADAQK
                     STDYLTDTYSEYDAAKELNPEEAEEDVNYLFDAFYYDFAFYDYFGGHAVFDQAKADTL
                     QEVQSRDSLTCEDLQKILVSHLTFIKDGHFNINQDYPSEKDIPFFFRQVMFVKTDSGY
                     QNANGKVVASVDDHPDLDELFKRSISEQGYLVYYPVLLKPATFDGTEWEKHTCDETLT
                     VHYTDGSTDTLTADAWSQYYKELPKDQNTDLRQTDGIPVFQFNSFEVSFLQEVNAAAA
                     QMSDAAISMLDLRSNVGGYEEVAHQWITRYSRQRVQGNGVRYDVVNPVSVAIQAHRWQ
                     THDNILILLSGKCSASSAEITMDLAYNLENSLIIGENTNGCMIGNGGHVELPNSKCSV
                     IMTSVCVYLLPEGTDYFEEMRGLSPDIWVPAKEAETLAAKLMENLK"
BASE COUNT          392 a          417 c          436 g          478 t
ORIGIN      
        1 tacattacac tggtttgcca gtaatgtgcg atcgtagctc agctggatag agcgttcggc
       61 tccgacccgg aaggccagag gttcgaatcc tcccggtcgc atgtgttaat aaaatccccg
      121 gtacacaaaa gtgcgccggg gatttttctt tgtggccggg agggtcagac cttactttag
      181 attttccata agttttgcgg ccagcgtttc ggcttctttg gccgggaccc agatatccgg
      241 agaaaggcca cgcatttctt caaaataatc tgtaccctcc ggcagcagat agacacatac
      301 ggatgtcatt ataaccgagc atttgctgtt cggcagttcc acatgcccac catttccgat
      361 catacatcca tttgtgtttt ctccgatgat caggctgttt tccagattat aggcaaggtc
      421 cattgtgatt tctgcagaag aggcagagca ttttccgctt aaaagaatca gaatgttgtc
      481 gtgcgtttgc caccgatgag cttgtatcgc tacggatacc ggattaacaa cgtcatagcg
      541 gactccattg ccctgtacac gctggcggct gtatcgggtg atccactgat gagctacttc
      601 ttcatatccg ccgacattgg agcgcagatc cagcatggag atcgcagcat cactcatttg
      661 tgctgccgca gcattgacct cctgtagaaa agagacctca aagctgttga actggaatac
      721 cgggattcca tctgtctggc gaagatcggt attctggtct ttgggaagct ctttgtaata
      781 ctggctccat gcgtctgctg tcagtgtgtc ggtactgccg tctgtatagt gcaccgtcag
      841 cgtttcatca caggtgtgct tttcccattc tgtcccatca aatgtagcag gcttcagcag
      901 gaccggataa tacaccagat acccctgctc agagattgaa cgcttgaaca gttcatccag
      961 atcgggatga tcgtccacgg atgcaacgac ttttccattg gcattctgat agcccgaatc
     1021 cgtttttacg aacatcacct gacggaagaa aaacggaatg tctttttccg acggatagtc
     1081 ctgattgatg ttgaagtgac cgtccttgat aaaggtaaga tgacttacca gaatcttctg
     1141 caaatcttca caggtcaggc tgtccctgct ttggacttcc tgcagagtat cagcctttgc
     1201 ctgatcgaat acagcgtgtc cgccaaaata gtcataaaag gcaaaatcat aatagaatgc
     1261 gtcgaagaga tagttcacat cttcttcggc ttcttccgga tttaattctt tggctgcatc
     1321 gtattcgctg taggtgtcag tgagataatc ggttgatttc tgggcatcag cggggtcatc
     1381 gtaatgccgt aggtatggag ttgggtcaga ggaaccgccc tcataacggc gatgctcttc
     1441 gttgatttct gcgagcagtt ccgctttggt tggctgcgcg tctttcacca gctgtgtgac
     1501 cttggtacag ccggaaaagc tcacgagcat gattagacag cagagcagag atgcaagttt
     1561 ggacttcatg gtgtaggact ccttttgatt ttgatattac cctttccgat ttttctgcca
     1621 gatgcggtat aggccgtccg tgatcagata ctcccggtat tcgatcggcg tgcggcaggc
     1681 ctcccggatg gtgcggggca ggttgccggt ggcataaaag cgg
//
LOCUS       DBMDQJ010000555         1713 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23328_length_1713_cov_1.293424, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000555 DBMDQJ010000000
VERSION     DBMDQJ010000555.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23328_length_1713_cov_1.293424"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="ACZO9R_10245"
     CDS             complement(<1..516)
                     /locus_tag="ACZO9R_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913475.1"
                     /translation="MEQPILSFLTAYFSDLHIQCRLTDRKQLELADLDLGLRNSILMQ
                     EADAQKQAPFPFVQNTIYFIVDYYECNYSFFRLPDKSAFLFVGPFLWREFERSQILSL
                     MNRLDIPTELFPQLQEYYHALPLIADKASMTALLRHVYQTLDADADVSVQTLHLKDLE
                     TRESFLEKHQFV"
     gene            729..>1713
                     /locus_tag="ACZO9R_10250"
     CDS             729..>1713
                     /locus_tag="ACZO9R_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-isomer specific 2-hydroxyacid dehydrogenase
                     family protein"
                     /protein_id="HIR0913476.1"
                     /translation="MKITAYEVRPDEKDYLLAAAQQEQIEITLVQGPMTQDNIDRAEG
                     ADGITILGDTKMDAALIEETAKRRIRVVATRTIGYDHIDLNAAKSNGVHVCNGYYDPD
                     GVAEYTVMLILMSLRNYKQALFRGNANDYSLGGLIGRELRNLTVGIIGTGKIGQKVAQ
                     NLQGFGCRILAYSRRQNADPALGIEYCSLEELYAKSDIISLHTPLSNQTRYMIDKKAI
                     DQMKDGVILINCARGELMRVDDIIEGIETRKIGALGLDVIEHEEGIYHMDRRSDILAN
                     RNMAYLRQFPNVTMTQHIAFYTEEAVKSMAESDGYADADSYLAATVGAGCNL"
BASE COUNT          474 a          373 c          478 g          388 t
ORIGIN      
        1 gacaaactgg tgcttttcca aaaaggattc tctggtctcg agatctttca aatgcagggt
       61 ctgtacggat acatccgcgt cggcatccag cgtctgatag acgtggcgca gcagggctgt
      121 catggaggct ttgtccgcga tcagcggcag tgcgtgataa tattcctgca gctgtggaaa
      181 aagttctgtc ggaatatcca gccggttcat aagtgacaga atctgggagc gctcaaattc
      241 tctccagaga aaaggcccga cgaacaggaa ggcactttta tccggcagac ggaaaaaaga
      301 ataattacac tcgtaataat ctacaataaa atagatggtg ttctgcacaa atggaaatgg
      361 cgcctgtttt tgcgcatcgg cttcctgcat caggatactg ttgcgcagtc cgagatccag
      421 atccgccagt tctaactgtt tgcggtcggt cagtctgcac tggatatgca ggtcagaaaa
      481 atatgcggta agaaaagata aaatcggctg ctccatcaag aacctcctgt ccgggtcagt
      541 gtctgttgtt ttgattttgg cacaatattt tctgttatgc aaggaaaaac aaaaaaatga
      601 caaggattat tcatcccttt aaagcatcgg cttgcagacc ggagcaattt atgataattc
      661 tataacaacg tgttcacggt gaacacattt tagaaaatag tgcagaatgc ttgaaagaga
      721 ggatacccat gaaaatcaca gcttatgaag tgagaccgga tgaaaaagat tacctgcttg
      781 ccgcagcaca gcaggagcag atagagatca cactggtgca ggggccgatg acacaggaca
      841 acatcgatcg tgctgaggga gcggacggca ttacgattct tggagataca aagatggacg
      901 ccgcattgat tgaggagact gcaaagcggc gtatccgcgt cgttgcgaca cgtaccatcg
      961 ggtatgacca tattgacctg aatgcggcga agagcaatgg tgttcacgtc tgcaacggct
     1021 actacgatcc ggacggcgtt gccgagtata cggtcatgct gattttaatg tcgcttcgca
     1081 attataagca ggcactgttc cgcgggaatg ccaacgatta ttcgcttggc gggctgatcg
     1141 gaagggagct tcgtaatctt accgtcggta tcatcggaac gggaaaaatc ggacagaagg
     1201 tggcacagaa tctgcaggga ttcggctgca ggattctggc atacagcaga cgtcagaatg
     1261 ccgatccggc gcttggaatc gaatactgtt ctctggagga actgtacgcg aagagtgata
     1321 ttatctcact gcacacgcca ctgtccaatc agacacgtta tatgattgat aaaaaggcga
     1381 tcgaccagat gaaggacggc gtcattctga tcaactgcgc gagaggagag ttgatgcgcg
     1441 tggatgacat catcgaggga atcgagacgc ggaagatcgg tgcgctggga ctggatgtga
     1501 tcgagcatga ggagggaatc taccatatgg accgccggtc ggatattctt gcaaaccgga
     1561 atatggcgta tctgagacag ttcccgaatg tgacgatgac gcagcacatt gcgttttata
     1621 cagaggaagc ggtaaaaagc atggcggaga gcgacggcta tgccgacgcg gacagctatc
     1681 tggccgcgac tgtcggcgcg ggctgcaacc ttg
//
LOCUS       DBMDQJ010000556         1713 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23336_length_1713_cov_0.768610, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000556 DBMDQJ010000000
VERSION     DBMDQJ010000556.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23336_length_1713_cov_0.768610"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /gene="nusB"
                     /locus_tag="ACZO9R_10255"
     CDS             <1..355
                     /gene="nusB"
                     /locus_tag="ACZO9R_10255"
                     /inference="COORDINATES: protein motif:HMM:NF013216.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR0913477.1"
                     /translation="LKLGGAFVNDAKNQNSGYPVLAWQDADAIAARFEKIKDKIPELD
                     KLLNENTEGWDTTRVGKVELAVLRLGAYELRYDDDIPDGVAINEAVEIAKKYGQESSG
                     GFVNAILAKIMKSGE"
     gene            357..1568
                     /gene="xseA"
                     /locus_tag="ACZO9R_10260"
     CDS             357..1568
                     /gene="xseA"
                     /locus_tag="ACZO9R_10260"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR0913478.1"
                     /translation="MQNVVSVRQLNTYIKNMFTQDYFLQTVFVKGEVSNCKYHPSGHI
                     YFSLKDAEGTISCVMFAGSRRGLAFPMRDGDNVIVGGSISVYERDGKYQIYAKEITKE
                     GAGLLYEKYLALKAELEEMGMFSPEYKQPIPFYNRKIGIVTAPTGAAIRDIMNISRRR
                     NPYVQLILYPALVQGEQAAESIVKGIQTLDAYGVDVMIVGRGGGSIEDLWAFNEEIVA
                     RAIFECRTPVISAVGHETDTTIADYVADLRAPTPSAAAELAVVDYRQLVESVRIFKNQ
                     LADRVEQKIVRNRDRARYLQARLLQASPQYQLREKRQYTADLNDKMRQAFSDKVKDRR
                     HRLALYAERLDGCSPLKKLQQGYSYTESPDGKALTSITQVKEGDAVTIHVTDGTVVAR
                     TEGIRSLERNG"
     gene            1570..>1713
                     /locus_tag="ACZO9R_10265"
     CDS             1570..>1713
                     /locus_tag="ACZO9R_10265"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR0913479.1"
                     /translation="MAKKKETAEQEQFRLEEAFDRIEALLEKLQDKDVTLEESFGLYQ
                     EGMG"
BASE COUNT          457 a          364 c          531 g          361 t
ORIGIN      
        1 gctgaagctc ggcggcgcat tcgtgaacga cgcgaagaat cagaacagcg gctatcccgt
       61 ccttgcctgg caggatgcgg atgccattgc agcccgtttc gagaagatta aggacaagat
      121 cccggaactg gataaactgt tgaatgagaa taccgaaggc tgggatacca cccgtgtggg
      181 aaaggtagaa ctcgcagtgt tacgtctggg tgcgtatgag ctgcgttatg atgacgatat
      241 cccggacggg gtagcgatca acgaggcggt ggagattgcc aaaaaatacg gtcaggagag
      301 ctccggcgga ttcgtcaatg cgatccttgc caagattatg aagtccggtg agtagaatac
      361 aaaatgtagt cagcgtacga caactgaata cttatatcaa aaatatgttc acgcaggatt
      421 actttttaca gacggtgttt gtaaaaggtg aggtaagcaa ctgtaaatac catccgtccg
      481 gccacatcta tttttcctta aaagatgcgg aaggaaccat ttcctgtgtc atgttcgccg
      541 gttcgcggcg cggccttgcc ttcccgatgc gggacggcga taacgtgatt gtcggcggca
      601 gcatcagcgt ctatgagcgg gacggaaagt atcagatcta tgcaaaagag atcacaaaag
      661 agggcgccgg ccttttatac gaaaagtatc tggccctaaa agcagaactg gaagagatgg
      721 gcatgttttc tccggagtat aagcagccga tcccatttta taatcggaag atcgggatcg
      781 tgacagctcc aacaggagcc gcgatccgtg atattatgaa tatcagcaga cggcggaatc
      841 cttatgtgca gctgatcctc tacccggctc tggtgcaggg tgaacaggcg gcggagagca
      901 ttgtaaaggg aatacagacg ctggatgctt acggtgtgga tgtgatgatc gtcgggcgtg
      961 gcggtggatc cattgaggat ctgtgggcat tcaatgagga gatcgtggcg cgtgctatct
     1021 tcgagtgtcg cacaccggtg atctcggcgg tgggtcatga gacggacacc accattgcag
     1081 attatgtggc ggatcttcgg gcaccgaccc catccgcagc agcggagctg gctgtggtgg
     1141 attaccgtca gctggtggag agtgtacgga tctttaagaa tcagctggca gaccgggtgg
     1201 aacagaagat agtaaggaac cgtgacaggg caagatacct gcaggcgcgg cttttgcagg
     1261 caagcccaca atatcagctt cgggagaagc ggcaatatac agcagatttg aatgataaaa
     1321 tgcggcaggc attttcagat aaagtaaaag accggcggca caggctggca ttatatgcgg
     1381 agcggctgga tggctgttcc cctttgaaga agcttcagca gggatactca tatacagaga
     1441 gccctgatgg aaaagcattg acctccatca ctcaggtgaa ggaaggggat gcagtgacca
     1501 ttcatgtgac ggacggaacc gttgtagcgc ggacggaagg tatccgcagt ctggaaagga
     1561 acggctgata tggcgaagaa gaaagagaca gcagagcagg agcagtttcg gctggaagag
     1621 gcatttgatc ggatcgaagc actgctggag aaactgcagg ataaagatgt gacactggag
     1681 gagtcctttg gtctctatca ggagggcatg ggg
//
LOCUS       DBMDQJ010000557         1712 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23339_length_1712_cov_1.723153, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000557 DBMDQJ010000000
VERSION     DBMDQJ010000557.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1712)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23339_length_1712_cov_1.723153"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            44..1195
                     /locus_tag="ACZO9R_10270"
     CDS             44..1195
                     /locus_tag="ACZO9R_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR0913480.1"
                     /translation="MNHTTDWGRRWRNTGYITFFLSGICAISSGIIVSILQGKYEFDY
                     GMTGTMLGLMSIGNMAASFAAGILPQKIGVKNTVLLLCSGYFLGYFLMAVTGMPGVLM
                     AAFLLVGFAKGGALNNCTILVKDNSADRTRSMNILHACYACGALLCPFLAASLLGIND
                     TLPMVGVAVTGLLLWMIFLTAGLPGAVKEKNGERGKISFAFLKDPKFWLITGLLFCQN
                     AAETSVTGWLVTYYKDMGILSGSFAAYTVTVMWGATLIARLLIAFVFPVHHIFRTLVW
                     MGGCCSVLYCGLVYMQHPAGAIAMLFAFAFAMAGVNPIAVAGAGREMNATSLGVMLPV
                     AGIGAIVMPWLIGVIADRVGLVTGMFCNLIPCVGILVFSVLILKYQAKN"
     gene            1335..>1712
                     /locus_tag="ACZO9R_10275"
     CDS             1335..>1712
                     /locus_tag="ACZO9R_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 2 galactose-binding
                     domain-containing protein"
                     /protein_id="HIR0913481.1"
                     /translation="MREVINFNTKWAFTKEATEVPKEMPEKWYWVTLPHSWNEIDGQD
                     GGNDYYRGTCYYAKQLKKSELPEADCYYLELRGANASADVYVNGKAVAHHDGGYSTWR
                     VDITKELTEEENLIVIAVENGVND"
BASE COUNT          415 a          343 c          520 g          434 t
ORIGIN      
        1 gtattgcggc tctctgtaag tggttatagg ggagaaggta cagatgaatc atacaacgga
       61 ctggggcaga agatggcgca acaccggcta tattacattc tttttgagcg gtatctgcgc
      121 aatatcgagc gggattattg taagtattct gcagggaaaa tatgaatttg actatggcat
      181 gacgggtacg atgctcggac tgatgagcat tggaaatatg gcggcgtcct ttgcggcggg
      241 tattctgccg cagaaaatcg gagtgaagaa tacagtactt cttctctgca gcggttattt
      301 cctggggtac tttctgatgg cggtgaccgg aatgccgggc gtgttgatgg cggcatttct
      361 gttggtcgga tttgcaaagg gaggagcact caataattgc acgatccttg taaaagacaa
      421 cagcgccgac cgtacccgca gcatgaacat actacatgcc tgctatgcgt gtggtgcgct
      481 gctgtgtccg tttcttgcgg cgtcgcttct tggaatcaac gacacgctgc cgatggtggg
      541 agtggcggtg acggggctgc ttttgtggat gatatttttg acggcgggac ttccgggcgc
      601 ggtcaaagag aagaacggcg aacggggaaa gatctcgttt gcatttttga aggatccgaa
      661 attctggctg attaccgggc ttttattctg ccagaatgcg gcggagacga gtgtgacggg
      721 atggcttgtg acatactata aagacatggg aattctctcg ggttcctttg cggcatacac
      781 ggtaacggtg atgtgggggg cgactctgat cgccagactt ctgatcgcgt tcgtttttcc
      841 ggtgcatcat attttccgca cgcttgtgtg gatgggcggc tgctgcagcg tgttgtactg
      901 cgggcttgta tacatgcagc atccggcggg agccatcgcc atgctgttcg cctttgcatt
      961 cgccatggca ggggtcaatc cgatcgccgt ggcgggagcc ggcagggaga tgaatgcaac
     1021 gagtctcggt gtgatgctgc cggtggcagg gattggcgcg atcgtgatgc cgtggctgat
     1081 cggtgtgatt gctgatcgtg tggggcttgt gaccggaatg ttctgcaatc tgattccgtg
     1141 cgtgggcatt ctggtttttt ccgtattgat cctgaaatat caggcaaaga attgaacttg
     1201 tttttcaaaa taaaggtaga atgctaaata ttgtgccatt tttgccaaaa actatctaaa
     1261 gaaaaaagtg cggttctgtg ttacgatgca gacagatcaa tgatgacgat attttttatg
     1321 gagggatgcg acacatgaga gaagtaatca attttaatac gaaatgggca tttaccaagg
     1381 aagccacaga agtgccgaag gagatgccgg agaagtggta ctgggtaaca cttccacatt
     1441 cctggaatga gattgacgga caggacggcg gcaacgatta ttaccgcgga acctgttatt
     1501 atgcaaaaca gttaaagaag agcgaactgc cggaggcaga ctgctattat ctggagttaa
     1561 gaggagcaaa cgcttccgca gacgtctatg tgaacggcaa ggcagtggca catcacgacg
     1621 gaggatattc cacctggcgt gtagacatca caaaagagct cacagaagaa gagaacctga
     1681 ttgtgatcgc tgtggagaat ggcgtaaacg ac
//
LOCUS       DBMDQJ010000558         1707 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23427_length_1707_cov_1.407223, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000558 DBMDQJ010000000
VERSION     DBMDQJ010000558.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23427_length_1707_cov_1.407223"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(16..645)
                     /locus_tag="ACZO9R_10280"
     CDS             complement(16..645)
                     /locus_tag="ACZO9R_10280"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR0913482.1"
                     /translation="MGNKIYTIGREFGSGGREIGEKLAERLGIKLYDKELLQQAAKES
                     GFCEEIFENHDEKPTSSFLYSLVMDTYSLNGYHSAPFLDMPLNHKVFLAQFDTIKKIA
                     ERESCVIVGRCADYALSDNPDCINIFIHADMDKRVKIISQRANITESKARDLIQKQDK
                     QRASYYNYYTSKKWGDSQSYHLTLDSGKLGIDGCIDMILKFREILDASR"
     gene            complement(723..1646)
                     /locus_tag="ACZO9R_10285"
     CDS             complement(723..1646)
                     /locus_tag="ACZO9R_10285"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR0913483.1"
                     /translation="MTRETFEAGIAYEGERLDKYLNLIFSETDAAQSRSFFQKLIKEG
                     HVLVNDTVQKANYRLKADDMICVEIPDAVQTPILPENIPLDILYEDDDLLVVNKPKGM
                     VVHPSAGHYTGTLVNAIMYHCRDSLSGINGEIRPGIVHRIDMDTTGSLIVCKNDESHV
                     FIAEQIKEHSVNRRYRGIVYGVVKEEEGTVNAPIGRHPIERKKMAINEKNGKPAITHY
                     KVLERFERYTYMEFKLETGRTHQIRVHMASIGHPLLGDTLYSSGRSPYKLQGQTLHAM
                     TIGFIHPRTREYMEVTAPLPEYFEKILKDLR"
BASE COUNT          406 a          424 c          373 g          504 t
ORIGIN      
        1 tattagccga tgatgttatc tggatgcatc gaggatttca cggaacttta agatcatatc
       61 aatacaaccg tcaattccca gtttaccgga gtccagtgtc agatgatagc tctgggaatc
      121 gccccatttt ttgcttgtat aatagttata ataacttgca cgctgcttat cctgtttctg
      181 gatcagatcc ctggctttgc tttccgtaat attggcgcgt tggctgataa tcttaacgcg
      241 tttgtccatg tccgcatgaa taaagatatt gatgcagtcc ggattgtccg ataatgcgta
      301 atcggcgcat cttccaacaa tcacgcagga ttcccgctct gcaatctttt tgatggtgtc
      361 aaactgcgcc aggaacactt tgtggtttaa aggcatatcg agaaacggtg ccgaatgata
      421 gccgttcagc gaatatgtat ccatgaccag ggaatacaaa aagctgctgg tcggtttctc
      481 atcatgattc tcgaaaatct cctcgcagaa accgctttcc tttgccgcct gctgtaacaa
      541 ttctttgtcg tagagcttaa ttccaagacg ttccgcaagc ttttcgccga tttctcttcc
      601 cccgctgcca aattcacgac caatggtata gattttgttg cccataaaag atcccgcctt
      661 tcctaacatt ctatgaatat attataatac atttttttga aaaacggaat tattttttga
      721 aattatcgca aatcttttag aattttttca aaatattctg gaagaggcgc cgtaacctcc
      781 atgtactctc tggttctcgg atgaatgaag ccgatcgtca tggcatgaag tgtctgcccc
      841 tgcagcttat agggacttct tccgctggaa tacagcgtgt cgccaagcag cggatgacca
      901 atgcttgcca tgtgcacgcg gatctgatgg gtgcgcccgg tctccagttt aaactccatg
      961 tatgtgtacc gctcaaagcg ttccagaacc ttgtaatggg tgatggcagg cttgccgttt
     1021 ttctcattga tcgccatttt cttgcgctcg attggatggc gtccgatcgg cgcattgacg
     1081 gtaccctcct cctctttcac cacaccgtat acgatgccgc ggtagcgccg gttcacggaa
     1141 tgttccttga tctgctccgc aataaagaca tggctctcat catttttgca tacaatcagt
     1201 gaacccgttg tatccatatc gatacggtgt acgatgcccg ggcggatctc cccgttgatg
     1261 ccggacaaac tgtccctgca gtgatacatg atggcattga caagtgtgcc ggtgtaatgc
     1321 ccggcggacg ggtggacgac cattcccttc ggcttgttga cgactaaaag atcgtcatcc
     1381 tcgtagagaa tgtcaagcgg aatattttcc ggaagaatcg gcgtctgtac cgcatccgga
     1441 atctcaacgc agatcatatc atccgccttt aaccggtagt tggctttctg caccgtatca
     1501 ttgacaagca catgcccctc tttgatcagt ttttgaaaaa aggagcgcga ctgcgccgca
     1561 tctgtctcgc tgaaaatgag attcagatac ttgtccagcc gctctccctc atatgcaatt
     1621 cccgcttcaa atgtttctct agtcattgtc ttttttctcc gaagaatgtt gtctggaaga
     1681 atccgaaggg aaaatctgtt caaaatc
//
LOCUS       DBMDQJ010000559         1700 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23550_length_1700_cov_0.914321, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000559 DBMDQJ010000000
VERSION     DBMDQJ010000559.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1700)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1700)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1700
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23550_length_1700_cov_0.914321"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            44..418
                     /locus_tag="ACZO9R_10290"
     CDS             44..418
                     /locus_tag="ACZO9R_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HIR0913484.1"
                     /translation="MKTNESAENYLETILVLSQKKPVVRGVDIAEELGFKKSSVSVAM
                     KNLREKEQIRVTPEGYIYLTDSGRAIAEMIYERHQLLSRGLMSLGVDEQTATEDACRI
                     EHVISQESFEAIKRHLHMTAEK"
     gene            complement(523..>1700)
                     /locus_tag="ACZO9R_10295"
     CDS             complement(523..>1700)
                     /locus_tag="ACZO9R_10295"
                     /inference="COORDINATES: protein motif:HMM:NF036578.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase-related protein"
                     /protein_id="HIR0913485.1"
                     /translation="QRNERMGWAGDTHIFCHTALNNSSLKKFYERNLQAMRDLQTPEG
                     QYPEIAPVGGGFGGITYECASIFMAWELYEQYGDIRTLEKFYPGMQKYMDYMKDKGLP
                     GTKVNPAIGPLGDWLAPEETDLLLLWNAFYYKEADLMSRIAGALGRTEEQHQYEALAA
                     KVKKFWNETFVLPDSGKTCNADGTLCDTQCSYAIALSYGVAEDRKRIGEHLIRKTRAI
                     GHTVGTGFFGTGILNQMLTEQGAVEDAWKMMLQTAFPSWLYPVTQGATTIWEHWDSYT
                     KEKGFGGQNAMNSFNHYSLGSVLSWLYHTGLGIQRDETKPGYQHILLKPQPGPLEFMK
                     GSVDSPYGVISAGWERKGDKIEYQVTIPVNTTATLFKNTQDPADTVELGSGTYTFML"
BASE COUNT          399 a          495 c          372 g          434 t
ORIGIN      
        1 ttatgaatct tcagctattt agaaaaacga ggtgaacatc ttgctgaaaa cgaacgaatc
       61 cgcagaaaat tatttggaaa ccattttagt cttaagtcag aaaaaacccg ttgtacgcgg
      121 tgttgatatt gcagaagaac ttggctttaa aaaatcaagt gtcagcgttg ccatgaaaaa
      181 cttaagagaa aaagagcaga tccgcgtaac gccggaaggc tacatctacc tgaccgacag
      241 cggacgcgcg atcgcagaga tgatctatga gcgccaccag ctcctctccc gcggcctgat
      301 gagccttggt gttgatgagc agaccgccac ggaagatgcc tgccgcatcg agcatgtcat
      361 cagccaggaa agctttgaag cgatcaaacg tcatctgcac atgactgccg aaaaataaac
      421 atgacaaaga aaacgctgcc ggagttctct tctctccgac agcgtttctc tttccgcccg
      481 aaagtctccc atttctttgc cggagtcttt cgggttttct ttttacagca taaaagtata
      541 cgttccgctt cccagttcca cggtatccgc cggatcctgc gtgtttttaa acagggttgc
      601 cgttgtattg accggaatcg taacctgata ctctattttg tcacctttcc gctcccatcc
      661 ggcagaaatc acgccgtacg ggctgtctac ggaacctttc ataaactcga gcgggcccgg
      721 ctgcggtttc agcagaatgt gctgataacc cggttttgtc tcgtctctct ggataccaag
      781 tcccgtgtga tacagccagg aaagaacgct tccgagagaa taatgattga acgagttcat
      841 cgcattctgt ccgccgaagc ctttttcctt cgtgtaggaa tcccagtgct cccagatcgt
      901 cgtcgctccc tgggtcaccg gatacagcca cgacgggaat gccgtctgca gcatcatctt
      961 ccaggcatcc tccacagcgc cctgctcggt cagcatctgg ttcagaatac ccgttccaaa
     1021 gaatcccgtt ccgaccgtat ggccgatcgc tctcgtcttt cggatcagat gctctcctat
     1081 tctctttctg tcttctgcca caccataaga cagtgcaatt gcatacgagc actgcgtatc
     1141 acacagcgtt ccgtccgcat tgcaggtctt tccggaatcc ggaagtacaa atgtctcatt
     1201 ccagaatttc ttcaccttcg cagcaagcgc ctcatactga tgctgctcct ctgttcttcc
     1261 gagcgcacct gcaatccggc tcatcagatc ggcttcttta taatagaatg cattccataa
     1321 aagcagcaga tccgtctctt ccggtgccag ccagtcgcca agcggtccga tcgccggatt
     1381 taccttcgtt cccggcagac ccttatcctt catatagtcc atatatttct gcatgccagg
     1441 ataaaatttc tccagtgtgc ggatatcccc atactgctca tacagttccc acgccataaa
     1501 aatactcgca cactcatacg taatgccgcc gaatccgccg cccaccggtg caatctccgg
     1561 gtactggcct tccggtgtct gcagatcacg catcgcctgc aggttccgct catagaattt
     1621 cttcaggctg ctgttgttga gcgccgtatg gcagaaaata tgcgtatcgc ccgcccagcc
     1681 catgcgctca tttctctgcg
//
LOCUS       DBMDQJ010000560         1697 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23579_length_1697_cov_1.550125, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000560 DBMDQJ010000000
VERSION     DBMDQJ010000560.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23579_length_1697_cov_1.550125"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            81..1688
                     /locus_tag="ACZO9R_10300"
     CDS             81..1688
                     /locus_tag="ACZO9R_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015569448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR0913486.1"
                     /translation="MKLEFLGAAHEVTGSCHYLEFADKHVLVDCGMEQGPDLYVNQEI
                     PVNASTIDYVFVTHAHIDHSGLLPMLYNHGFRGQIFTTTATHQLCEIMLKDSAHIQMF
                     EAEWKNRKAKRAGRPEVVPLYDMNDAVGVLGHFVSCDYHSVINVCEGLKVRFVDAGHL
                     LGSSSIEMWISENTAEGVEERKIVFSGDIGPGNRPLIKDPEYLTSADYVVMESTYGDR
                     THETPPDYAVELARVIRDTFTRGGNLVIPAFSVGRTQEMLYFIRRIKMENLLPEFQNF
                     EVYVDSPLAVEATTIFGKNVQDCFDDTALALVQQGINPIGFPGLRMAITSDESKMINF
                     NDKPKVILSASGMCEAGRIRHHLKHNLWRKDSTILFVGYQVPGTLGNMLLNGAKEVKL
                     FGETIEVQAKIENLPGISGHADVNQLTKWVSMFDPKPKRVFIVHGEDKVTEQFAAHIH
                     EELGLEAYAPFSGDAFDLLTGACVAQGSREAVEKKSTRAVNNIFARLVAAGERLMTVI
                     RKCEGMPNRELGKFADQINELCNKWER"
BASE COUNT          404 a          382 c          537 g          374 t
ORIGIN      
        1 agagacaagg gaaaagaatt gtaaccgcag gacattacgt gtataatggt cttatcaaaa
       61 aaatgctggg gggaatgcat atgaagcttg aatttttggg ggctgcacac gaggtgacag
      121 gaagctgtca ttatctggag tttgcggata agcatgtact ggtggactgc ggtatggaac
      181 agggaccgga tctgtatgtc aatcaggaga taccggtgaa cgcgtcgacg atcgactatg
      241 tgtttgtgac acacgcgcac atcgatcact ccggtctgct tccgatgtta tataatcatg
      301 gcttccgcgg gcagattttt acgacgacgg cgacacatca gctgtgcgag attatgctca
      361 aggacagcgc gcatatccag atgtttgagg cggagtggaa gaaccgcaag gcgaagcgtg
      421 ccggcaggcc ggaggtcgta ccgctctacg atatgaatga tgcggtgggg gttctgggac
      481 attttgtatc ctgcgactac cacagcgtga tcaacgtctg tgagggctta aaagtccggt
      541 ttgtggatgc ggggcatctg cttggatctt ccagtattga gatgtggatc tctgagaaca
      601 ctgctgaggg tgtggaggag cgcaagatcg tattttccgg agacatcgga ccggggaacc
      661 gcccgctcat caaggatccg gagtatctta catcggcgga ttacgtggtc atggagtcga
      721 cgtacggtga ccgcacgcat gagacaccgc cggattatgc cgtggagctt gcccgcgtga
      781 tccgcgatac gtttacgaga ggcggcaatc tggtcatacc ggcgttctcg gtgggacgta
      841 cgcaggagat gctgtatttt atccgtcgga tcaagatgga gaatctgctt ccggaattcc
      901 agaattttga ggtgtatgtg gacagtccgc tcgcggtgga agccaccacg attttcggca
      961 aaaatgtgca ggactgtttt gatgacaccg cgctcgcact tgtgcagcag ggcatcaacc
     1021 cgatcggttt tccagggctt cggatggcga tcaccagcga cgagtcgaag atgatcaatt
     1081 ttaacgacaa gccgaaagtc attctctctg cgtccggcat gtgcgaggcg gggcggatcc
     1141 ggcatcatct gaagcacaat ctatggagaa aggattccac gatcctgttt gtcggatatc
     1201 aggttccggg cacgctcggg aatatgctgt taaacggggc aaaggaagtg aagctctttg
     1261 gcgagacgat cgaggtgcag gcgaagatcg agaaccttcc gggaatcagc ggccacgcgg
     1321 acgtgaacca gctgactaaa tgggtgagca tgtttgaccc gaagccgaag cgcgttttta
     1381 tcgtacatgg cgaagacaag gtgacggagc agtttgcggc gcatattcac gaggagctgg
     1441 ggctggaggc ttacgcgccc ttcagcggtg atgcgtttga tctgctgaca ggcgcctgtg
     1501 tggcacaggg ctccagagag gcggtggaga agaagagtac ccgcgcagtc aacaatatct
     1561 ttgcaagact tgtggcagcc ggcgagcgcc tgatgacggt gatccgcaag tgcgagggta
     1621 tgccgaaccg cgaacttggc aaatttgcgg accagatcaa tgaactctgc aacaagtggg
     1681 agcggtaggc attacat
//
LOCUS       DBMDQJ010000561         1691 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23691_length_1691_cov_1.945912, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000561 DBMDQJ010000000
VERSION     DBMDQJ010000561.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23691_length_1691_cov_1.945912"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            201..791
                     /locus_tag="ACZO9R_10305"
     CDS             201..791
                     /locus_tag="ACZO9R_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913487.1"
                     /translation="MQTLHLTGINKLLHPERDKRSATERIFDHLSHSNLGLNRGEATA
                     DTGSAASALDSFSVTQNISELLSPACGMSEEEKKAYLAKIIAKLKSGKKLTSEEMRFL
                     QAEDPQLYQQAARVQAMRGSLESGLAHSTSKEEAQSVYLDALTHISEEDPMKEYIIAA
                     YDDAMKEFQKSDQYQSLPETKEDAAKQHTGSRHSHS"
     gene            complement(776..>1691)
                     /locus_tag="ACZO9R_10310"
     CDS             complement(776..>1691)
                     /locus_tag="ACZO9R_10310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR0913488.1"
                     /translation="EKGMFIAESPKVIERALDAGYMPVSILMEKQHIEGEGQKILARC
                     GEIPVYTAEFDVLTKLTGFQLTRGMLCAMYRQPLPSVRSVCKGAKRIAVLENVVNPTN
                     VGAIFRSAAALGMDAVLLTTGCSNPLYRRAIRVSMGTVFQVSWTYLGEETKPETVRNL
                     DPEEEKEPGTGIERNPDPEEEKEPGTGIERNSEPEEKADSGNWQKQLHELGFHTVAMA
                     LKEDSLSIDAPKLMNEDKLAIVLGTEGDGLAPETIAACDDTVCIPMAHGVDSLNVAAA
                     SAVAFWQLGRQAHKDNCCNMNSNYQLWE"
BASE COUNT          391 a          503 c          355 g          442 t
ORIGIN      
        1 aacggctttt ccgccagagt tccctttttc tcctgtgcgc ttctctttaa aggcagcatt
       61 atgattttat ggcaattatt tcccgctagg aatccgtgac agttttttcg gaattcatga
      121 cagctttctt ggattcacga tatttttcat tggacagtta tttctcatat catatttttt
      181 tcacatgaaa ggattttttt atgcagacac ttcatctgac agggatcaat aaacttcttc
      241 atccggagcg cgacaaacgc tccgccaccg agcgcatttt tgaccatttg agccactcaa
      301 acctcggatt aaaccgcggc gaagcaacgg ctgataccgg ctctgcggca tcggcgctcg
      361 acagtttttc tgtcacgcag aatatctcgg agcttctcag tcccgcctgc ggcatgagcg
      421 aggaagagaa aaaagcctac cttgccaaaa tcattgcgaa attaaagagc ggcaaaaagc
      481 ttacctcaga agagatgcgc tttctgcagg cggaagatcc acagctatac cagcaggcgg
      541 ctcgtgtcca ggcaatgcgt ggctcccttg agtcgggtct tgcgcacagc acttccaaag
      601 aggaggcgca atctgtctat ctggacgcgc tcacccatat cagcgaggaa gatcccatga
      661 aagaatacat catcgcagcc tacgatgatg ccatgaagga attccagaaa tccgaccagt
      721 accagtcgct gccagagaca aaagaggatg ccgcaaagca gcacaccggt tcccgtcatt
      781 cccatagctg ataatttgag ttcatgttgc agcaattatc tttgtgtgcc tgtcttccaa
      841 gctgccagaa cgcaaccgca ctggcagctg caacatttaa agaatcgacg ccgtgcgcca
      901 ttggaatgca taccgtatca tcgcatgctg cgatcgtctc cggtgccaga ccatctccct
      961 ctgttcccag tacaattgca agcttgtcct cattcataag cttcggagca tcaatactta
     1021 aggaatcctc tttcaatgcc atcgccaccg tatgaaatcc cagttcatga agctgcttct
     1081 gccaattccc ggaatctgct ttctcttccg gctccgagtt tcgttctatc cctgttcccg
     1141 gctctttctc ttcttccgga tccggatttc gttctatccc cgttcctggc tctttctctt
     1201 cttccggatc cagatttctc acagtctccg gctttgtctc ctctccaaga tatgtccacg
     1261 acacctgaaa caccgtcccc atactcacac ggattgctct ccggtaaagc ggattgctgc
     1321 accctgtggt cagcagcacc gcatccattc caagcgccgc agcagagcgg aaaatagcgc
     1381 ccacgttcgt cggatttacc acattttcca aaactgcaat tcgttttgcc cccttgcaga
     1441 cactccgcac cgaggggagc ggctgccggt acatcgcaca gagcattcct ctggtcagtt
     1501 gaaaccccgt cagcttcgtg agtacatcaa actccgccgt gtagaccggg atctcgccgc
     1561 accgcgcaag aatcttctgt ccttccccct caatatgctg tttttccatc agaattgaca
     1621 ccggcatata gcctgcgtcc agcgccctct cgataacctt gggactttcc gcaataaaca
     1681 tgcctttttc c
//
LOCUS       DBMDQJ010000562         1685 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23813_length_1685_cov_1.042929, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000562 DBMDQJ010000000
VERSION     DBMDQJ010000562.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23813_length_1685_cov_1.042929"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..146
                     /locus_tag="ACZO9R_10315"
     CDS             <1..146
                     /locus_tag="ACZO9R_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HIR0913489.1"
                     /translation="LKNNVPRYLVIDFSKAEQETTASDEDVLSISKRLMEQNKEAYEV
                     LAK"
     gene            173..286
                     /locus_tag="ACZO9R_10320"
                     /pseudo
     CDS             173..286
                     /locus_tag="ACZO9R_10320"
                     /inference="COORDINATES: protein motif:HMM:NF014691.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
     gene            complement(333..1193)
                     /gene="rsmI"
                     /locus_tag="ACZO9R_10325"
     CDS             complement(333..1193)
                     /gene="rsmI"
                     /locus_tag="ACZO9R_10325"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR0913490.1"
                     /translation="MAGKLYLCATPIGNLEDMTFRAIRILQEVDLIAAEDTRNSIKLL
                     NHFEIHTPMTSYHEYNKYDKGRELVQKLLAGTNIALITDAGTPGISDPGEELVKMAYE
                     AGIEVTSLPGACACVTALTLSGLSTRRFVFEAFLPPDKKEHKLALERLKNETRTMIVY
                     EAPHHLLKTLRELLETLGNRRLTLCRELTKKHETAWQTTIEDAITRYEQEDPKGECVL
                     VIEGRSQKEIEDEAKAAFEEISIEEHMKRYEDQGIARKEAMKLVAKDRGVTKRDIYQY
                     LLGKESYNDF"
     gene            complement(1198..>1685)
                     /locus_tag="ACZO9R_10330"
     CDS             complement(1198..>1685)
                     /locus_tag="ACZO9R_10330"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HIR0913491.1"
                     /translation="RSVRYNHLENDVEIFQGDVKTASQQFGKEVFDVVTCNPPYMTGQ
                     HGLTNALEAKTIARHEVLCSFDDIAKEASAVLKTKGRFYLVHRPFRLAEIFSTLKKYH
                     LEPKRMQLVYPFVDKEPNMVLIEAMKGGNPRITVEKPLIVYETPGVYTKDIRELYGDG
                     R"
BASE COUNT          375 a          505 c          347 g          458 t
ORIGIN      
        1 ttctgaaaaa taacgttcca agatatcttg tcatcgactt cagcaaagcc gagcaggaaa
       61 ccactgcatc cgacgaggat gtgttaagca tctcaaaacg cctgatggaa caaaacaaag
      121 aagcatacga ggtacttgct aaatgaaaag actgagtaaa aagcagattc tcatgctgca
      181 tactcagttg attcagcaaa ctggcggaag cgacggagtc agagattaca atctgctgga
      241 ctccgctctc gaaacgcctt tccagtcctt cggcggagaa gacttgtacc ccaccctgca
      301 ggcaaaagcc gcaagactgg gctatggcct gatcaaaaat cattgtatga ttctttcccc
      361 agcaaatact gataaatatc cctcttcgtc accccgcggt ccttcgccac gagtttcatc
      421 gcttcttttc ttgcaatccc ctgatcttca taccgtttca tatgttcttc gatcgaaatc
      481 tcctcaaacg ctgccttcgc ctcgtcctcg atttctttct ggcttctgcc ctcgatgaca
      541 agaacgcatt cgcctttcgg atcttcctgt tcgtagcgag tgatggcatc ttcaatcgtg
      601 gtctgccatg ccgtctcgtg tttcttggtc agttcccggc agagtgtcag ccgccggttg
      661 ccaagcgtct ccagcagctc ccgcagcgtt ttcagcagat gatgcggcgc ctcgtatacg
      721 atcatcgtcc gcgtctcgtt tttcagccgt tccagcgcca gtttatgttc ttttttatcc
      781 ggcggaagaa atgcttcgaa gacaaatctt ctcgttgaaa gtcccgacag tgtcagtgcc
      841 gtcacacacg cgcatgcgcc cggcaggctt gtcacttcga tgcctgcctc gtacgccatc
      901 ttcaccagct cctcacccgg atcagaaatt cccggtgttc cggcatccgt gatcagcgcg
      961 atatttgttc ccgccagtag cttctgcacc agctcgcggc ccttgtcgta tttgttgtat
     1021 tcgtgataac tcgtcatcgg cgtatgaatc tcaaaatggt tcagcagctt aatgctgttt
     1081 ctcgtatcct ccgccgcgat cagatcgacc tcctgcagaa tgcggattgc acggaaggtc
     1141 atgtcctcca gatttccaat tggtgttgca cacaaatata attttcctgc catgttctta
     1201 tctcccgtct ccatataatt cccggatgtc ctttgtatac acgcccggcg tctcatacac
     1261 aatcagcggt ttctccacgg taattctcgg attcccgcct ttcatcgcct cgatcagcac
     1321 catgttcggc tccttgtcca caaacggata aacgagctgc atcctttttg gttccagatg
     1381 atattttttc agcgtcgaaa aaatttccgc cagccggaac ggccggtgca ccagatagaa
     1441 ccgtcctttc gtctttaaaa ccgccgatgc ctctttcgca atatcatcaa acgagcacag
     1501 cacctcatgc cgcgcaatcg tcttcgcctc aagcgcattc gtcagcccgt gctgccccgt
     1561 catgtacggc ggattgcacg ttaccacatc aaacacttct tttccaaact gctgcgatgc
     1621 cgtcttcaca tctccctgga aaatctcaac atcattttcc agatgattat accgcacact
     1681 ccgct
//
LOCUS       DBMDQJ010000563         1684 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23820_length_1684_cov_1.678459, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000563 DBMDQJ010000000
VERSION     DBMDQJ010000563.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23820_length_1684_cov_1.678459"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(226..>1684)
                     /gene="hcp"
                     /locus_tag="ACZO9R_10335"
                     /gene_synonym="priS"
     CDS             complement(226..>1684)
                     /gene="hcp"
                     /locus_tag="ACZO9R_10335"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HIR0913492.1"
                     /translation="GMRTCGNIRRGQSRHILIEGLFTTITNVNFNDETLREMIAKVHA
                     EKERVVPNCATCASPCGNTSDYDMKEIWEADEDIRSLKSLILFGIRGMAAYAYHAAVL
                     GYEDETVNAFFYKALSFLGFDLEMGDLLPVVLEVGEVNLKCMELLDRANTETFGNPKP
                     TKVPLTVEKGPFIIITGHDLYDLKLLLEQTKDKGINVYTHGEMLPAHAYPLLKQYPHL
                     KGNFGTAWQNQQKEFDGVPAPILYTTNCLMPVKESYRDRVFTTEVVSYPQMVHIGADK
                     DFTPVIEKALELGGYKEDTEFTGINGGKEVMTGFAHGTVLSVADQVVDAVKSGAIRHF
                     FLVGGCDGARPGRNYYTEFVKQTPKDSVILTLACGKYRFNDLDLGEISGLPRLMDMGQ
                     CNDAYSAIKVAVALAEAFGCSVNELPLSMVLSWYEQKAVCILLTLLHLGIQNIKLGPT
                     LPAFISPNVLAYLVEHYHIAPITTPEADLKEILGA"
BASE COUNT          404 a          489 c          379 g          412 t
ORIGIN      
        1 ctcgccaccg cacaaaacga ttttgatgcg gcgcgctttg ccggatttag cgaggaagag
       61 ctcatctgct atgcgaaagt gacggaaaag atcaaggaaa ataccatgcg ggtgctaggc
      121 gtctagcgcc cgtccgtgtt ttttagatac cgtaacaaaa aagacaagta caaactgctt
      181 ttttgtacct gccttcttcg attagaatga aattttcccg ttcccttacg ctccgagaat
      241 ctcctttaag tctgcctctg gtgtcgtgat cggtgcaata tggtaatgct ccaccagata
      301 tgctaacaca ttcggggaga taaacgccgg aagtgtcggt cccagcttga tgttctggat
      361 gcccagatgc agcagcgtca aaaggataca gaccgccttc tgctcatacc aggacaatac
      421 catggataac ggcagttcat tgaccgagca gccaaacgcc tcggcaagcg ccaccgcaac
      481 cttgatcgcg ctgtacgcat cattgcactg tcccatatcc atcaggcgcg gcagaccact
      541 gatctccccg agatccagat cgttaaagcg gtattttccg catgcaagcg ttaagatcac
      601 actgtccttc ggggtctgtt ttacaaattc ggtatagtaa ttgcgtcccg gtctggcacc
      661 gtcacagccg cccaccagga agaaatgacg gattgcgccg ctctttaccg catccaccac
      721 ctgatccgca acggagagca ccgtgccgtg tgcaaatccg gtcatcacct ctttgccgcc
      781 gttgatgccg gtgaactccg tatcttcctt gtaaccgccg agctctaacg ctttctcgat
      841 caccggcgta aagtccttgt ccgcaccgat atgcaccatc tgcggataag aaaccacctc
      901 tgttgtgaat acacggtcac ggtagctctc cttcaccggc atcaagcagt tcgttgtata
      961 cagaatcggc gccggaaccc cgtcgaactc cttctgctga ttctgccatg ccgttccaaa
     1021 gtttcccttt aaatgcggat actgctttaa cagcggatac gcatgtgccg gaagcatctc
     1081 accgtgcgta tagacattga ttcccttatc cttcgtctgc tccaaaagca gttttaaatc
     1141 gtagagatcg tgaccggtga tgatgataaa cggtcccttc tccacggtaa gcggcacctt
     1201 tgtcggcttc ggattgccga atgtctctgt gttggcgcgg tctaaaagct ccatacactt
     1261 taaattcacc tcgccgactt ccagaaccac cggaagcagg tctcccatct ccagatcaaa
     1321 gcccaggaac gaaagcgcct tatagaaaaa tgcattgacc gtctcatcct catagccgag
     1381 caccgccgca tgataagcgt aggcagccat gccccggatg ccaaataaaa tcaaagactt
     1441 cagggaacgg atatcctcat ccgcctccca gatctccttc atgtcgtaat ccgaggtatt
     1501 gccgcacggc gatgcgcagg tggcacagtt cggaaccacg cgttccttct ccgcatgaac
     1561 ctttgcgatc atctcacgca gtgtctcatc attaaagttg acattcgtga tcgttgtgaa
     1621 taatccttct attaaaatat gtcgggattg tcccctccgg atattgccgc acgttctcat
     1681 accc
//
LOCUS       DBMDQJ010000564         1682 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23846_length_1682_cov_1.909551, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000564 DBMDQJ010000000
VERSION     DBMDQJ010000564.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23846_length_1682_cov_1.909551"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="ACZO9R_10340"
     CDS             <1..621
                     /locus_tag="ACZO9R_10340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HIR0913493.1"
                     /translation="KQKLPKFVDATPYDIQVLTPMRKGLLGVERLNGILQRYLNPPSP
                     QKREKEHGDILFREGDKIMQTRNNYQLEWEIRTKYGLSVDKGTGVFNGDMGIVREIND
                     FAETMTVEFDEGRMVEYPYKLLDELELAYAITIHKSQGSEYPAVVIPLLSGPSMLMNR
                     NLLYTAVTRARKCVTLVGNEVTFAQMVQNTSQQKRYSGLCDRLKEA"
     gene            complement(1232..>1682)
                     /locus_tag="ACZO9R_10345"
     CDS             complement(1232..>1682)
                     /locus_tag="ACZO9R_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR0913494.1"
                     /translation="SWGITKKERDIYREMLATLDLGLEDRLSSKVGLLSGGQRQAITL
                     LMASIQKPRLLLLDEHTAALDPKTAAKVLEISDKIIADHHLTAMMVTHNMKDAIAHGN
                     RLIMMHEGRVILNIAGEEKKKLTVEDLLHQFEKVSGEEFANDKALLS"
BASE COUNT          417 a          419 c          460 g          386 t
ORIGIN      
        1 aagcagaaac tccccaaatt tgtggatgcg acgccgtacg atatacaggt gttgacgccg
       61 atgcgcaagg gactgctggg cgtagagcgg ttaaacggaa tactgcagcg ctatctgaat
      121 ccgccgtctc cgcagaagcg tgaaaaggaa cacggcgata ttctgttccg tgaaggagac
      181 aagatcatgc agacacggaa caactaccag ttagagtggg aaatccgcac aaaatacggt
      241 ctgtctgtgg ataaaggaac gggagtattc aacggagaca tgggaattgt ccgggagatc
      301 aacgatttcg cggagacgat gactgtggaa tttgatgagg gcagaatggt ggaatatcca
      361 tacaaactgc tggatgaact ggaactggcg tatgcgatta ccatacataa gtcgcaggga
      421 agcgagtacc cggcggtcgt cattccgctt ttgtccggac cgtcgatgct tatgaaccgg
      481 aatctgctgt acacagcggt cacccgcgct cggaaatgtg tgacgctggt cggcaatgaa
      541 gtaacatttg cacagatggt acagaacacg tcgcagcaaa aacgatacag cgggctgtgt
      601 gaccgcctga aggaggcata acactgccgt ggaatatttt gggagaaaaa gatatccggg
      661 gaaaacgaat gcaggcgaga agcccttcgt gtttcaggat gcaacgggca cagtcccttc
      721 tttggagcaa aatcccgtat ttcttccgtg cgatcgttgt cttaggttcc gtgtctttgc
      781 cacgtttgaa caccccacgg aagctgtccg tcaagcagca gacaatgagc ggactttctg
      841 gcagcactaa ctgcactcac gcatagacat cataaaaccg cgcgaaccgg aactcgccgg
      901 catctgctat tgaaaatagg gcagatgccg actcagacat cggcttcgcg cggttatgtt
      961 ttatgcttag agtgcaggta agtgctgcac aaaagtccta aaattgtctg tagcttgcgg
     1021 acagcttccg cgggggtgct ccaaacttgg aaagacacgg atcatcgcaa aggcaacggc
     1081 tgcacggatt agaaatacac gattttgcga ttagaaggga ttgtgcccgt tacatcctga
     1141 aacacaaaag gcttccgcct gcgctcactt catccggaca gcttcttctc ccgaaatatc
     1201 ctgcgaccgc gttatgcctt tttccctgcg gttacgacag cagcgcttta tcgtttgcaa
     1261 attcctcgcc gctgaccttc tcaaactgat ggaggagatc ttccaccgtc agctttttct
     1321 tctcttctcc ggcaatgttt aagatgaccc ggccttcatg catcatgatg aggcggttgc
     1381 cgtgcgcgat ggcgtccttc atgttatggg tgaccatcat ggcggtcagg tgatgatctg
     1441 caatgatttt gtcggaaatc tcaagcacct tggcggctgt cttcggatcg agtgccgcgg
     1501 tgtgctcgtc taaaagcaga agcctcggct tctggatgga agccatgagc agcgtgattg
     1561 cctggcgctg accgccggac aacaacccta ctttgctgga aagccggtct tccagaccga
     1621 gatctaaggt ggcaagcatc tcacggtaga tgtcgcgctc cttcttcgtg atgccccagg
     1681 ag
//
LOCUS       DBMDQJ010000565         1681 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23870_length_1681_cov_1.384177, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000565 DBMDQJ010000000
VERSION     DBMDQJ010000565.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23870_length_1681_cov_1.384177"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            48..1196
                     /locus_tag="ACZO9R_10350"
     CDS             48..1196
                     /locus_tag="ACZO9R_10350"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR0913495.1"
                     /translation="MYKIMTAGPTQVRENVRRARSFVTTNPDLDETFVDFYKETCELI
                     SRLLHTGNETLILGGEGILGLEAACASLTEPGDPVLVLDNGVYGRGFADFVSMYGGKP
                     VLYPVDEKNPIDPAALEEYLKEHHDYKYATLVHCDTPSGMLNDISALCPLLKKYGILT
                     VVDSVSGMFGEDVRVDDFQIDVLCGGSQKAVSAPPGLTFVTVSDAAWAAIDGRNVPIA
                     SFYANLKVFEGYYEKKWFPYTMPISDIYGLRAAFDNIAGDPDMLARHARIGEACRAAV
                     RAVGLNLHLASGFSNTVTVFDVPKETTADAILQTMRQKHGIMLAGSFDSLAGQVIRIG
                     HMGSNANVVDMIETLDALDDTLRELNVPLKGQLRMIFQEEVSKKGLVL"
BASE COUNT          417 a          378 c          476 g          410 t
ORIGIN      
        1 aagcaaatcg cggcgagata ctaaagctgc aaaaggagga aacggttatg tataagatta
       61 tgacagcggg accgacacag gttcgcgaaa atgtcagaag agcaaggagc tttgtgacga
      121 ccaatccgga tctggatgaa actttcgtag atttttataa ggagacctgt gaactgatca
      181 gccgcctgct gcacaccggg aatgagaccc tgattttagg cggagaggga attctggggc
      241 ttgaggctgc ctgtgcctcc ctgacggaac cgggcgatcc ggtactggtg ctggataacg
      301 gcgtatacgg cagagggttt gctgatttcg tttccatgta tggcggaaag ccggttctgt
      361 atcccgtgga tgagaaaaat ccgattgatc cggccgccct ggaagaatat ttaaaggaac
      421 atcacgatta caaatatgca acgctcgtgc actgcgatac cccgagcggc atgttaaatg
      481 acatctctgc gctttgtccg cttctgaaaa aatacgggat actcacggtg gtggattccg
      541 tctccggcat gttcggcgag gatgtgcgcg tggatgattt tcagattgat gtcctctgcg
      601 gcggttccca gaaagctgtt tcggcgcctc cggggctgac gtttgtcacg gtgagtgatg
      661 cggcatgggc agccatcgat ggcagaaatg taccgattgc ctctttttat gcaaacctta
      721 aggtgtttga gggatattac gagaaaaaat ggtttccgta caccatgccg atcagtgata
      781 tctacggact gcgcgccgcg ttcgacaata tcgcgggcga tccggatatg ctggcgcgtc
      841 atgcacggat cggtgaggca tgcagggcgg cagtccgcgc agtgggactg aacttacacc
      901 ttgcatccgg tttctccaat accgtcacgg tgtttgacgt cccgaaagag acgaccgcgg
      961 atgccatttt acagaccatg cgtcagaagc acggtatcat gctggcagga tcgtttgact
     1021 ccctagcggg gcaggtgatc cgtatcggac acatgggtag caatgcgaac gtggtggaca
     1081 tgatcgagac gttagatgca ttggatgaca ccctgaggga actgaatgtt ccgctgaaag
     1141 ggcagcttcg catgatcttt caggaggaag tgtccaaaaa ggggctggta ctttagaggg
     1201 cagtgaattt tgaagcgata agtaatatga tagacagctt atctgttgat ttcagctgga
     1261 atgcatagga tacattctgt tcaaaaaatg cggcatttct aaccagccgg gatttgcatc
     1321 tgcgatgatc cgtgtctttc caagtttgga acactccacg gactgtgtcc gcaagccaac
     1381 agacaatttt aggacttttg tgcgtgcccc ttgtgccacc actgagcaaa acataaccgc
     1441 gcgaagccga tgtttgaatt ggcatctgcc atgttttcaa tagcagatgc caacgagttt
     1501 cggttcgcgc ggttttatat tgtctgtgaa gtggtggcac taggggcact aaaaagtccg
     1561 ctcattgtct gtggcttgtc ggacacacac cgggagagtc caaacgtgga agagacacgg
     1621 aatcagatgc aaacctccgg caggaagaaa tgctctcatt tttttcaaat aattgtatcc
     1681 t
//
LOCUS       DBMDQJ010000566         1678 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23928_length_1678_cov_1.077362, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000566 DBMDQJ010000000
VERSION     DBMDQJ010000566.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23928_length_1678_cov_1.077362"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            9..161
                     /locus_tag="ACZO9R_10355"
     CDS             9..161
                     /locus_tag="ACZO9R_10355"
                     /inference="COORDINATES: protein motif:HMM:NF025422.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4250 domain-containing protein"
                     /protein_id="HIR0913496.1"
                     /translation="MLLSFVNTQLRDHYPSLEEFAATYQIDAAEVISALKNIDYEYDP
                     ATNQFI"
     gene            complement(189..764)
                     /locus_tag="ACZO9R_10360"
     CDS             complement(189..764)
                     /locus_tag="ACZO9R_10360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR0913497.1"
                     /translation="MRERTYEIKIEGVPIIVTKKRMKNMYLRISKEDGTVRISAPHQM
                     SDARIAAFARERIEWIRKYQQKYKEAGERRSEKQELDAAEIARRKKQLKREVERLVAK
                     WEPVMRVKVSGITIRQMKTRWGSCNVQTHHININLALLSKPPECLEYVVVHEMTHILE
                     ASHNQRFWGFMTKFYPDWKRVRKYLSDETVD"
     gene            complement(761..>1678)
                     /locus_tag="ACZO9R_10365"
     CDS             complement(761..>1678)
                     /locus_tag="ACZO9R_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR0913498.1"
                     /translation="VTTQEVIDYLEANGIRSERKTVYTDMEELEDFGLDIIRVKERPG
                     GYYLASRKFELAELKLLVDAVQASKFITTKKSRELIAKLETLCGREQAKQLHRQVVVT
                     NRSKAVNENIYYNVDMIYNAIAENVKIRFQYFEWTVNKEMKLRRDGSYYEVSPWLLSW
                     DDENYYLIAYDDRSRDIRHYRVDKMLKIELTAESREGKEQFGNFDVAAYSRKTFGMFA
                     GEDETVTLICEDALTGVMIDRFGKEVAMRRYDETRIRVRVSVAVSRQFFGWLAGLGAA
                     VRIESPEPVVREYREYLETILKTYGEQEL"
BASE COUNT          341 a          526 c          348 g          463 t
ORIGIN      
        1 atcccgtcat gctcttaagc tttgtcaaca cacagcttcg tgaccactac ccgtcgctgg
       61 aagaatttgc tgcaacctac cagattgatg ccgcagaggt tatctctgca ttaaaaaata
      121 tagactacga gtacgatccg gcgaccaatc agtttatctg atccgtcagc cccgcttctt
      181 ccggcacgct aatccacagt ctcgtcactt agatattttc gcacgcgctt ccagtccggg
      241 taaaacttcg tcataaatcc ccagaagcgc tgattgtggc tcgcctccag aatatgtgtc
      301 atctcatgta ccacgacata ctcgaggcac tccggcggct tggacaagag cgccagattg
      361 atgttgatgt gatgtgtctg cacattgcag gacccccatc tcgtcttcat ctgccggatg
      421 gtgattccgg agactttcac cctcatcacc ggctcccatt ttgccacaag acgctccacc
      481 tcacgcttta actgtttctt tctgcgcgcg atttctgccg catccagttc ctgtttttcc
      541 gaacggcgct ctccggcttc tttgtatttc tgctgatatt tccgtatcca ttcgatccgt
      601 tctctggcaa atgccgcaat ccgcgcatca ctcatctgat gcggcgctga gatccgcact
      661 gtaccgtcct ctttactgat gcgcagatac atgtttttca tccgtttttt cgtgacaatg
      721 atcggcacgc cctctatctt tatctcataa gtgcgctctc tcatagttcc tgctccccgt
      781 atgttttcaa aatagtttcc agatactcgc ggtattcccg cacaacaggt tccggcgact
      841 cgatccgcac tgccgcgcca agtccagcca gccagccgaa aaactggcgg ctgaccgcga
      901 cactcacccg cacccggatt cttgtctcat cataccggcg catcgcgacc tccttgccga
      961 aacggtcgat catgactccc gtgagggcgt cttcgcagat taaggtcaca gtctcgtcct
     1021 cccccgcgaa catgccgaac gtttttcggg aatatgccgc cacgtcaaag ttcccgaact
     1081 gctccttccc ctctctgctt tccgccgtaa gttcgatctt tagcatttta tccacacggt
     1141 aatggcggat gtcgcggctt ctgtcgtcgt aggcgatcag ataatagttt tcgtcatccc
     1201 acgagagcag ccagggactc acttcatagt aactgccgtc ccgccgcagt ttcatctctt
     1261 tattgaccgt ccattcaaaa tactgaaaac ggatcttcac attttccgcg atggcattgt
     1321 aaatcatatc tacattataa taaatgtttt cattgaccgc cttgctgcgg ttggtcacca
     1381 cgacctgcct gtggagctgc tttgcctgtt ccctgccgca gagcgtctcc agcttggcaa
     1441 tcagctctct cgacttcttc gtcgtgatga acttcgacgc ctgcacggca tccacgagaa
     1501 gttttaactc cgccagttca aatttgcggc tggcaagata atatccgccg ggtcgctcct
     1561 tcacccgtat aatatccagc ccgaaatctt ccagttcctc catatcggtg tacactgtct
     1621 tgcgctccga acggattccg ttcgcctcca gatagtcgat cacctcctgc gtcgtcac
//
LOCUS       DBMDQJ010000567         1675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23987_length_1675_cov_1.515248, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000567 DBMDQJ010000000
VERSION     DBMDQJ010000567.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23987_length_1675_cov_1.515248"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="ACZO9R_10370"
     CDS             <1..1038
                     /locus_tag="ACZO9R_10370"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR0913499.1"
                     /translation="KNPGDSVTTTLNYDLQLQAYDALGSYDGAVVVMEPETGRILAMV
                     SKPDFDPNTIADDWDTFVSDDSDSSVLLNRATQGLYPPGSTFKIFTTLEYIHENADYA
                     DYHFDCSGEYTVGTKTIHCHNNKRHGSEDLKTSFANSCNSSYASLGLTLDLEQFDDLC
                     DQMLFNTALPTDFESSKSSFVLEPGDSDSAVMETSIGQGKTLVTPFHMALIASAIAND
                     GVLMKPYVIDRITDAEGEVVEQYEPAEYKTLLSESDASVLQEYMRYVVEEGTATKLKS
                     DSYEAAGKTGSAEFSSSSDATHSWFVGYAHNGEEPDIAVAVIVENSGVGSEYAVPIAK
                     QIFDAYYTWEN"
     gene            1119..1571
                     /locus_tag="ACZO9R_10375"
     CDS             1119..1571
                     /locus_tag="ACZO9R_10375"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012199368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR0913500.1"
                     /translation="MIEATSCGGVVIFRGKILLLYKNYKNKYEGWVLPKGTVEAGEEY
                     PDTATREVLEETGVKAAIIKYVGKSQYSFTVPEDTVEKDVHWYLMMADSYYSKPQREE
                     YFVDSGYYKYHEAYHLLKFPNEKQILEKAYNEYLELKKSNLWGNHKYF"
BASE COUNT          456 a          334 c          500 g          385 t
ORIGIN      
        1 aaaaatccgg gggacagtgt cacaacgacg ttgaattatg atctgcagct tcaggcgtac
       61 gatgcactgg gaagctatga cggggcggtc gttgtgatgg agccggagac aggcaggatc
      121 cttgccatgg tgtcaaagcc ggatttcgat ccgaacacga tcgcggatga ctgggatacc
      181 tttgtgtcag acgacagtga cagctcggtt cttctaaacc gtgccaccca ggggctgtat
      241 ccgccgggat cgacgtttaa gatctttacc acgctcgagt acattcatga gaatgcggac
      301 tatgcggact atcattttga ctgcagcggc gagtataccg tgggcacaaa gaccatccac
      361 tgccataaca ataagcggca cggatcggag gatttaaaga cctccttcgc aaattcgtgt
      421 aattcctcct atgcgtcctt aggactcaca ctggatctgg aacagtttga tgatctctgt
      481 gaccagatgc tgtttaatac cgcgcttccg acggatttcg agtcgtcgaa gagcagcttt
      541 gtgctggagc cgggagacag cgattcagcg gtgatggaga cgtccatcgg acagggtaag
      601 acgcttgtga caccgtttca catggcgtta atcgcttctg cgatcgccaa cgacggtgtg
      661 ctgatgaagc cgtatgtgat tgaccgcatc acggatgcgg agggcgaggt tgtggagcag
      721 tatgagcctg cggagtacaa aacactgctc tcggagagcg atgcttccgt tttgcaggaa
      781 tatatgcgct atgttgtgga ggaaggaaca gcgaccaagt taaagagcga cagctatgag
      841 gcagccggaa agacgggttc ggcggagttc tcatccagct cggacgccac acattcctgg
      901 tttgtgggat atgcgcataa cggggaggaa ccggacatcg cggtggcggt gatcgtggag
      961 aattccggcg tgggaagcga gtatgcggtc ccgattgcaa aacagatctt tgatgcgtat
     1021 tatacatggg agaactaagc gcatacgagg ttgcgtgaag tctgaaaaag tattatactg
     1081 gatataatta tgaaaagacc acatcgggag gtattgcaat gatagaggca acgagctgtg
     1141 gcggtgtggt aatattccgt ggtaagatat tgcttttata caagaattat aagaacaagt
     1201 atgaaggctg ggttctgccc aaggggacgg tggaagccgg ggaggagtat ccggatacgg
     1261 caacgcgtga ggtgttggag gagaccgggg tgaaggcggc aattatcaaa tatgtcggca
     1321 agagccagta ttcctttacg gtaccggagg acacggtgga gaaggacgtc cactggtatc
     1381 ttatgatggc agacagttat tacagcaagc cgcagcggga agaatatttt gttgattccg
     1441 gatattataa gtatcacgaa gcgtatcatc tcttgaagtt tccgaacgaa aaacagattc
     1501 tggagaaagc ttacaatgaa tatctggagc ttaagaagag taatttgtgg ggaaatcaca
     1561 aatactttta gagcagtgtg atatggagag catgcatgac atggtatgac gagttatatg
     1621 taggggaaag tattgtacat aagacaaagc gcgtgaagtg gaaaatcaac cacaa
//
LOCUS       DBMDQJ010000568         1675 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_23994_length_1675_cov_1.161372, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000568 DBMDQJ010000000
VERSION     DBMDQJ010000568.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23994_length_1675_cov_1.161372"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            232..1389
                     /locus_tag="ACZO9R_10380"
     CDS             232..1389
                     /locus_tag="ACZO9R_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR0913501.1"
                     /translation="MKLFHLSDLHIGKQLHHYNLAAEQRKTLAQIVALAKSEHPDAIL
                     IAGDIYDTPVPSAEAVSVFDAFLSALCEIEPQIAVMIIAGNHDSARRIDFASSILAKH
                     EVYITGLPPMQEGETIRRVTLADGYGEVDFYLLPFVKPGYVRKLFDEEIKGYDMAVRR
                     LIEREEIDPARRCVILSHQFYTAGGKEPATCDSEIHYVGGVENVDAGVLAPFDYAALG
                     HIHRAQHIGAEKYRYCGAPFPYSISEAGEEKSVTVVELGEKGQPPVIRQIPLPPVREV
                     KKLTGTLHEILEQAGEEVCHAFVSITLTDDVEAFQPKERLEEKYDHILEIRIDNARTR
                     KLLSDTEEQLAEVRPEEAFAMFFSEMNGREMTPEERELMRQVIAQEMEGDE"
     gene            1386..>1675
                     /locus_tag="ACZO9R_10385"
     CDS             1386..>1675
                     /locus_tag="ACZO9R_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004847585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0913502.1"
                     /translation="MKPEKLILSAFGSYAGRTEIDFTAQTGGLFLITGDTGAGKTTIF
                     DAITYALYGEASGGGKSGAMMRSQYAKSVTETYVEFFFLYAGEAYRVRRNPE"
BASE COUNT          409 a          369 c          530 g          367 t
ORIGIN      
        1 cattcttcac aatatttata gttcccaaat aaatcctgtg gttgttccat cgtctgtcac
       61 ctccggtagt atgctgccac atccgtccgc cgcatgcagg catgggcgtt cgtctttact
      121 ccatgttcac tgtttataga ttcattatac catgaacgac ggtgaatgaa aactttttat
      181 cacgtagaaa agaaaagaaa cttagcagaa gcttagcggg gaggaaacga tttgaaactg
      241 tttcatttgt cggatcttca tataggaaaa cagctgcatc actacaatct cgcggcagag
      301 cagcgcaaga cactggcgca gattgtggcg cttgcaaaaa gcgagcaccc ggatgccatc
      361 ctgattgcgg gagatattta cgacacgccg gtgccttcgg cggaggcagt gtctgtcttt
      421 gatgcgtttc tgtcagcatt atgcgagata gagccgcaga ttgcagtcat gatcattgcg
      481 gggaatcatg acagtgccag aagaattgat tttgccagca gtattcttgc aaagcatgag
      541 gtgtatatca cgggacttcc gccgatgcag gagggggaga cgatccgccg cgtgacgctt
      601 gcggacggat acggtgaggt ggatttttat ctgcttccgt ttgtgaagcc gggttatgtg
      661 agaaagctgt ttgacgagga aataaaaggc tatgacatgg cggtaaggcg cctgattgag
      721 cgcgaggaga tagatccggc gcgaagatgc gtgatcctgt cacaccagtt ttatacggcg
      781 ggcggaaagg agcctgcgac ctgtgactcc gaaatccact atgtcggtgg cgttgaaaat
      841 gtcgatgccg gcgtgcttgc gccgttcgac tatgctgcat tgggacatat ccaccgcgca
      901 cagcatatag gagcggaaaa ataccgctat tgcggggcgc cgtttccgta ttccatcagc
      961 gaggcggggg aggagaagag cgtgacggtc gtggaactgg gagaaaaggg acagccgcct
     1021 gtgatccggc agattccgct tccgccggtg cgggaggtaa aaaagctgac cgggacgctg
     1081 catgagattt tggagcaggc gggggaggag gtctgccacg catttgtgag catcaccctc
     1141 acggatgatg tggaagcgtt ccagccgaag gagcgtctgg aggaaaaata cgaccacatc
     1201 ctggagatac ggatcgacaa cgcgcggacg aggaagctgt tgtcggacac ggaagagcag
     1261 cttgccgaag tgcggccgga ggaggcgttt gccatgtttt tttcggagat gaatgggaga
     1321 gagatgacgc ctgaggagcg ggaactgatg cgtcaggtga ttgcgcagga aatggagggg
     1381 gacgaatgaa gcctgaaaaa ctcatcctgt cggcgttcgg atcgtatgcg ggacgcacgg
     1441 agattgattt tacggcacag acgggcggac tgttcctgat cacgggggat accggcgccg
     1501 gtaagactac gatttttgat gccatcacct atgcactgta cggggaggcg agcggcggag
     1561 gcaaaagcgg agccatgatg cggagccagt atgccaaaag cgtgacggag acctatgtgg
     1621 aattcttttt tttgtatgcg ggcgaggcgt accgcgtgcg gagaaatccg gaata
//
LOCUS       DBMDQJ010000569         1667 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24138_length_1667_cov_1.086207, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000569 DBMDQJ010000000
VERSION     DBMDQJ010000569.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24138_length_1667_cov_1.086207"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="ACZO9R_10390"
     CDS             complement(<1..1361)
                     /locus_tag="ACZO9R_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015567923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR0913503.1"
                     /translation="MTIKEAASAWGITERRVNELCKAGRISGAYKEGRQWFIPDGTQK
                     PMDKRGRRSAVKPAVSSARKPLPIGVSDYRDACKNYYYVDKTLMIKEFLDERPKVSLF
                     TRPRRFGKTLNMDMLRTFFEKTTEDTSVYFRDKKIWFCGESYRAHHRKYPVIFLSFKD
                     VKYTSWEETYQTLQKLIAQEFRRHDALASSSALSDYEKEEYSLLATEAADEVEYQMSL
                     RTLTLLLHKHHTVAPIVIIDEYDTPIQQGHVSGFYDTIIGFMRNLFSGGLKDNPHLSY
                     GFLTGILRVAKESIFSGLNNLKINSILDERYSKYFGFTSSEVHEMADYYHASDKYQEL
                     CEWYDGYLFGNTEIFNPWSVIGYFNNNCRPKAFWQSTGSNDIIREVLASATPDIMERL
                     ELLMKGESFVTHIDTGVIYPQIQNDPSSVYSFLLVAGYLKAVSCDQSYGRISMYIASV
                     PLG"
BASE COUNT          431 a          407 c          404 g          425 t
ORIGIN      
        1 attcccaagg gcacgctggc aatgtacatg gaaatcctgc cgtaagactg gtcgcagctc
       61 acagccttca aataacctgc caccagcagg aagctgtaaa cagaggacgg atcattctgg
      121 atctgcggat aaatgacgcc ggtgtcaatg tgcgtaacga aggattcgcc cttcatcaac
      181 agctccagac gctccataat atccggcgtt gcagaagcca gcacctcacg gatgatgtca
      241 ttgcttccgg tggactgcca gaatgctttg ggacggcagt tattattgaa gtatccaatg
      301 acagaccagg gattaaaaat ctcggtattg ccgaaaaggt aaccatcata ccactcgcaa
      361 agctcctgat atttatcaga agcatgatag tagtctgcca tctcgtgtac ttcgcttgaa
      421 gtgaagccga aatatttact gtagcgctcg tctaaaatcg agttgatctt cagattgttc
      481 aggccactga agatactttc cttggcaaca cgcaggatgc cggtcaggaa accatagctc
      541 agatgcggat tgtctttcag accaccggag aacaggtttc gcatgaaccc gatgatcgtg
      601 tcgtagaagc cgctcacatg gccctgctga atgggagtat catattcgtc aatgatgacg
      661 ataggtgcga cagtgtggtg cttatggagg agcagcgtca gtgttcgcag ggacatttga
      721 tattccactt cgtcagcagc ttctgttgca agaagggaat attcctcttt ttcgtaatca
      781 ctaagcgcag agctggatgc caacgcgtcg tggcggcgga attcctgcgc aatcagcttt
      841 tggagcgtct gataggtttc ttcccaagaa gtatatttca cgtccttaaa agacaggaaa
      901 atcacaggat actttctgtg atgggcacgg taggattccc cgcaaaacca gattttcttg
      961 tctctgaagt aaacggatgt gtcctcggtc gtcttttcga agaatgtacg gagcatgtcc
     1021 atgttcagcg tcttgccaaa acgtctggga cgggtgaaca gggatacctt ggggcgctca
     1081 tccagaaatt ccttgatcat cagggtcttg tccacatagt agtagttctt gcaggcatcc
     1141 cggtaatcag acacaccaat aggcaagggc ttgcgtgcgg atgacactgc gggcttcaca
     1201 gcacttcggc ggccacgctt atccatcggc ttttgagtac cgtccgggat aaaccactgc
     1261 ctgccctcct tatatgcgcc agagatacgt ccagctttgc aaagctcatt gacccgtcgt
     1321 tcggtaatac cccaagcgga agccgcttct ttgatcgtca tattggaaca cctccattgc
     1381 agtccgtcaa gaacattata accactattc cgaataaaat caactgtatt attccgaatg
     1441 ataacggcgt tattcggaac ggttttattc tgcgaatatt ctgttctttc agtagctatt
     1501 tcctgctttc tgtgatattg ttcgtttgcc aaaaggaggc gatgatatgc agaaagaaaa
     1561 taatctcgcg gtcgggcaat ggtgcgacca ctggttcacc gctaaccgcc acaaatggaa
     1621 tagcaacacc gagggcggat accgcaatct gatttacagc cacatca
//
LOCUS       DBMDQJ010000570         1663 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24202_length_1663_cov_1.412292, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000570 DBMDQJ010000000
VERSION     DBMDQJ010000570.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24202_length_1663_cov_1.412292"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="ACZO9R_10395"
     CDS             <1..299
                     /locus_tag="ACZO9R_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913504.1"
                     /translation="GMLVLHRIYKIGQDGLYLLGDHQTAIEGPVRREQVKGKMTGMVR
                     DGRYMDVGNPGYRMLSVVWLWLRPARRAIMVPAARLRRMVRFALFRRNNKRYNK"
     gene            328..>1663
                     /gene="glgA"
                     /locus_tag="ACZO9R_10400"
     CDS             328..>1663
                     /gene="glgA"
                     /locus_tag="ACZO9R_10400"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - alpha-1,4-glucan
                     glucosyltransferase (UDP-glucose donor) activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HIR0913505.1"
                     /translation="MMKILFAASEAVPFMKTGGLADVTGSLPGYFDKKHFDVRIIMPK
                     YLCMDEKWKAQLRFVCHFYVNLSWRRQYVGIFETVQNGITYYFVDNEFYFAGDKPYHN
                     IYEDVEKFAFFSKAVLEALPYLDFWPDVIHCHDWQTGLVPVFLRTFYGDQRCYSNIRT
                     VFSVHNLKFQGRFSLPAVIDITGLPEQIFSSDKLESYGEANYLKGGVVYADAVTTVSP
                     TYAREIMTPEGGEGLDGLMRAREGSLYGILNGIDIREYDPQTDAYLANHYNETNHTEG
                     KGLNKLKLQKLLGLPVDKDVFLLGMVSRMTDQKGFDLIAYVMDELMSTERLQLVVAGT
                     GEARYEEMLRYFAEKYPQKIHVTIGYSEELAHRIYGSCDAFLMPSLFEPCGLSQLMSL
                     RYGTVPIVRETGGLKDTVMPYNEYEQTGTGFSFANYNAHEMLGVLRYAMRVYY"
BASE COUNT          399 a          349 c          515 g          400 t
ORIGIN      
        1 atggaatgct ggtactgcac cggatttata agatcgggca ggacggactg tatctgctgg
       61 gggatcacca gacagccatc gagggacccg tgcggcggga gcaggtcaag gggaaaatga
      121 ccggtatggt aagagacgga cgctatatgg atgtggggaa tccgggatac cggatgttaa
      181 gtgtggtgtg gctatggctc agaccggcgc gcagagctat tatggtgcct gccgcgcggc
      241 tgcggcgcat ggtaagattc gcacttttca gaagaaacaa taagcgctat aataaataga
      301 gggatcaaaa agcctggggg tgattacatg atgaagattt tatttgcagc ttcggaagca
      361 gtgccgttta tgaaaacggg tggtctggcg gatgtgaccg gatcgctgcc ggggtatttc
      421 gataagaagc attttgatgt gcgcattatc atgccaaagt atttatgcat ggatgaaaag
      481 tggaaggcac agctgcggtt tgtctgccat ttctatgtga atctcagctg gcggagacag
      541 tacgtcggta tctttgaaac ggtacagaac ggcattacat actattttgt ggacaatgaa
      601 ttctactttg cgggagataa gccgtatcac aatatctatg aggacgtgga gaagttcgca
      661 ttcttctcga aagcggtgtt agaggcgctg ccgtatctgg atttctggcc ggatgtcatt
      721 cactgccatg actggcagac gggactggta ccggtattct tgcggacatt ttacggggat
      781 cagagatgtt attccaatat ccgcacggtc ttttccgttc acaatctgaa gtttcaggga
      841 aggttctcac tgccggcggt tatagatatt accggtctgc cagagcagat cttttcttcg
      901 gataagctgg agtcctacgg ggaggccaac tacctgaagg gcggtgtggt gtatgccgat
      961 gcggtcacga cggtgagccc cacatatgcg agagagatca tgacgccgga gggcggagag
     1021 ggattggacg gtctgatgcg cgcccgggaa ggcagcctgt atggaatctt aaacggcatt
     1081 gatatcaggg aatatgatcc gcagacagat gcctatctgg cgaaccatta caacgagacc
     1141 aatcacacgg aggggaaggg actcaataag ctaaagcttc agaagctttt gggactcccg
     1201 gtggataagg acgtattttt acttggaatg gtgtcccgga tgacggatca gaagggattt
     1261 gacctgattg cctatgtgat ggatgaactg atgagcaccg agcgtctgca gcttgtggtg
     1321 gcgggtaccg gtgaggcgcg ctacgaggag atgctgcgct attttgcgga aaaatatccg
     1381 cagaagattc acgtgacgat cggctactcg gaggagcttg cacaccgcat ctacggctcc
     1441 tgcgatgcgt ttttaatgcc gtcgctgttt gagccgtgtg gtctgtcgca gctgatgagt
     1501 cttcgctatg gaaccgtgcc gatcgtgcgc gagacgggcg ggctgaaaga tacggtgatg
     1561 ccgtacaatg aatacgagca gaccggcaca ggattctcct ttgccaatta taatgcgcat
     1621 gaaatgctgg gcgtcctccg ctatgcgatg cgcgtgtatt aca
//
LOCUS       DBMDQJ010000571         1658 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24291_length_1658_cov_1.630058, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000571 DBMDQJ010000000
VERSION     DBMDQJ010000571.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1658
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24291_length_1658_cov_1.630058"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            169..>1658
                     /locus_tag="ACZO9R_10405"
     CDS             169..>1658
                     /locus_tag="ACZO9R_10405"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR0913506.1"
                     /translation="MADYSQKEKELEREHLEKLVKEEDRSIKPMKEFESPEDLYHELI
                     ASVRKYHPSADISLIEKAYHVARDAHEGQTRKSGEPYIVHPLCVAIILAELELDKETI
                     AAGLLHDVLEDTVMTADEIKEEFSEEVLLLVDGVTKLRHLHLADNSHNHDQKDRSAER
                     LEMQAENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYQSKEAQIRIARETQEIYCPI
                     AQRLGISKIKIELDDLSLKYLEPEAYYDLVRQIDQRKSVRDDYVQSLVEEVRAHIKAA
                     GIVAEIEGRAKHFFSIYKKMVNQDKTLDQIYDLFAIRIIVDSVKDCYAALGVIHEMYK
                     PIPGRFKDYIAMPKPNMYQSLHTTLIGPTGQPFEIQIRTYEMHRTAEYGIAAHWKYKE
                     ASNNGGAAVPMTVTEEEKLSWLRQILEWQRDMSDNKEFMSLLKSDLDLFSDTVFCFTP
                     TGDVKNLPNGSTPIDFAYSIHSAVGNKMIGAKANGKLVPIDYVIQNG"
BASE COUNT          461 a          361 c          473 g          363 t
ORIGIN      
        1 ttttagaaaa agcggaatgg tgtctgatgt aacgcttacg atgcctgacg gagaagtttt
       61 tgtcgggaaa aataaagaga cagatgagaa aatacaaagt tgcattttat taggaaataa
      121 aatataatat tgaaataaaa attatcatgg atgaagtggg tgaggcacat ggcagattac
      181 tctcagaaag aaaaagaact tgagcgtgag catttggaaa aactggtaaa ggaagaggac
      241 cgcagtatca agccaatgaa ggagtttgag agtccggagg atttgtatca tgagctgatc
      301 gccagtgtgc gcaaatatca cccttccgca gacatttctc ttattgaaaa ggcgtatcat
      361 gtggcgcggg acgcgcatga gggacagacg cgcaaatctg gtgagccgta tattgtgcat
      421 ccgctgtgcg tggcaatcat tttagcagag ctggagcttg acaaggagac gatcgcggca
      481 ggtctgcttc atgatgtgct tgaggacaca gtcatgacgg cagatgagat caaggaggag
      541 ttcagcgagg aagtgctgct tctcgtggat ggcgtgacga agctgcgcca tctgcatctc
      601 gcagacaaca gccataatca cgatcagaag gaccgttctg cggagcgctt agagatgcag
      661 gcggagaacc tgcgcaagat gttcctcgcc atggcaaagg atatccgggt catcttaatc
      721 aagcttgcag accggcttca caatatgcgt accttaaagt accagtccaa ggaggcacag
      781 atccgcatcg cgcgtgagac gcaggagatc tactgcccga ttgcacagcg gctcggtatt
      841 tcaaagatca agattgaact tgacgacctc tcgttaaagt atctggagcc ggaggcatac
      901 tacgaccttg tgcgtcagat tgaccagcgc aagagtgtgc gcgacgacta cgtgcagtcg
      961 ctcgtggagg aagtgcgcgc gcatatcaag gcggcgggca tcgtggcaga gattgaggga
     1021 cgcgcgaagc attttttcag catctacaag aagatggtga atcaggataa aacgctcgac
     1081 cagatctatg atctgttcgc catccgcatc atcgtggatt ctgtcaagga ctgctatgcg
     1141 gcgcttggtg tgattcacga gatgtacaag ccgatcccgg gccgttttaa ggattatatc
     1201 gcgatgccga agccgaacat gtaccagtcg cttcatacga cgttaatcgg accgaccgga
     1261 cagccgtttg agatccagat ccgtacctat gagatgcacc ggacagcgga atacggtata
     1321 gcggcgcact ggaaatataa ggaagcgagc aacaacggcg gcgcggcagt gccgatgact
     1381 gtcacggagg aggagaagtt aagctggctg cgccagatct tggagtggca gcgcgacatg
     1441 tctgataata aggagttcat gagcctttta aagagtgatc tggatctttt ttcggacaca
     1501 gtattctgct ttacaccgac cggcgatgtc aagaacctgc cgaacgggtc gacaccgatt
     1561 gattttgcgt acagcattca ctctgcggtc ggcaacaaaa tgatcggtgc aaaagccaac
     1621 ggaaagctgg ttccgatcga ttatgtcatc cagaacgg
//
LOCUS       DBMDQJ010000572         1648 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24456_length_1648_cov_1.239819, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000572 DBMDQJ010000000
VERSION     DBMDQJ010000572.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1648)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24456_length_1648_cov_1.239819"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="ACZO9R_10410"
     CDS             complement(<1..324)
                     /locus_tag="ACZO9R_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein"
                     /protein_id="HIR0913507.1"
                     /translation="MNGIAALLEQGRYLAAQTDAFPSTTETVEALNHAQEGIEEVAKN
                     PGIIRTWLEGLVPDLLNFAFQILIALLVYVIGGRIIKWITKLVRRSMDRAGTDEGVKQ
                     FMIPVI"
     gene            complement(358..1599)
                     /locus_tag="ACZO9R_10415"
     CDS             complement(358..1599)
                     /locus_tag="ACZO9R_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HIR0913508.1"
                     /translation="MAEIRPFVSIRPQRGKAAEIAALPYDVYNREEATKEVERHPQSF
                     LSIDRAETQFPLSVDMYDDKVYERAHDTLWQWVENGTFLTEDKKCYYLYELTMNGRVQ
                     TGVAACASIDDYENGIIKKHENTRAEKEVDRIRHVDVCNAQTGPIFLAYRANDGIRRI
                     VEKTKQGEPEYDFTSGDGVRHRVFVIREDADIAAIHEAFAGIDSIYIADGHHRAASAV
                     KVGQKRRKEHPGYTGEEEFNYFLSVLFPDDELMIMDYNRVIRDLNGYSFKGLYREVEK
                     RFDVEEITGAEDTRPKERGTFCMYMEGHWFSCHIRQEDRTPEDPVADLDVSILQNKLL
                     GPVLGIRDPKTDGRIDFVGGIRGMDELVRRCEQDCAVAFAMYPTSIAELFAVADAGLL
                     MPPKSTWFEPKLRSGLFIHEI"
BASE COUNT          347 a          530 c          346 g          425 t
ORIGIN      
        1 gatcaccggt atcatgaact gcttgacgcc ctcatcggtt cccgcccggt ccatcgagcg
       61 ccgcaccagc tttgtgatcc acttgatgat cctgcctccg atcacataca caagcagtgc
      121 gatcagaatc tggaatgcga agtttaacag atccggcacc agcccttcca gccatgtacg
      181 gataatcccc ggattctttg ctacctcctc aattccctcc tgtgcatggt tcaatgcctc
      241 cacggtctcc gtggtcgacg ggaatgcatc tgtctgtgcc gccagatacc ttccctgttc
      301 caaaagtgcc gctatcccgt tcactctctc gtcctccatc caactttata atccttctta
      361 aatttcatga ataaacagac cgctccttag cttcggctcg aaccaggtcg atttcggcgg
      421 catcaaaagt cccgcatctg ccaccgcgaa cagttccgcg atggacgtcg gatacattgc
      481 aaacgccacc gcgcagtcct gctcacagcg ccgcaccagt tcgtccatgc cccggattcc
      541 gccgacaaaa tcaatgcgtc catccgtctt cggatctctg atgccgagca ccggtccaag
      601 aagcttattc tgcagtatgg aaacatccag atctgcaacc ggatcttccg gcgtgcggtc
      661 ctcctgccgg atatggcagg aaaaccagtg tccttccata tacatgcaaa aggttccccg
      721 ctccttaggt ctggtatcct ctgccccggt aatctcctca acgtcaaacc gcttctcgac
      781 ttcacggtac aatcccttaa aagaataccc gttcagatcg cgtatcacac ggttgtaatc
      841 cataatcatg agttcatcat ccggaaacaa taccgagaga aaatagttaa actcctcctc
      901 cccggtataa cccggatgtt ccttccggcg cttctgccct acttttaccg cggacgctgc
      961 ccggtggtgt ccgtctgcaa tgtagatgct gtcaatgccc gcaaacgctt cgtggatcgc
     1021 agcaatatcc gcatcctcgc ggatgacaaa cacccggtgt ctcacgccgt cccccgatgt
     1081 gaaatcatat tccggctctc cctgcttcgt cttttccacg atacgccgga tcccgtcatt
     1141 cgcgcggtac gccaaaaaga tcggtccggt ctgtgcgtta cagacatcca catggcggat
     1201 gcggtcgacc tccttctccg cgcgggtatt ctcgtgcttt ttgatgatgc cattctcata
     1261 atcatcgatg gacgcgcagg ctgccactcc cgtctgcact cttccgttca tggtaagttc
     1321 gtagaggtaa taacactttt tatcctccgt cagaaacgtg ccgttctcca cccattgcca
     1381 gagtgtatca tgcgcacgct catacacctt gtcgtcgtac atatccaccg acagcgggaa
     1441 ctgtgtctcc gcgcggtcga tgctaaggaa agactgcgga tgccgctcca cttccttcgt
     1501 agcttcctct ctgttataaa catcataagg gagggcagca atctctgctg ccttccctct
     1561 ctgtggtctg atgcttacaa atggtctgat ttctgccatt gttctcacac ctcgttattt
     1621 tactttccgt acttttaata ccccatcc
//
LOCUS       DBMDQJ010000573         1638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24634_length_1638_cov_1.599219, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000573 DBMDQJ010000000
VERSION     DBMDQJ010000573.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24634_length_1638_cov_1.599219"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(67..762)
                     /locus_tag="ACZO9R_10420"
     CDS             complement(67..762)
                     /locus_tag="ACZO9R_10420"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:NF013786.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR0913509.1"
                     /translation="MTNIIALCDEGLYEELLRYVPTERQEKIARCGKRQDRMRSLAAG
                     LLLEYGLRSMGCSLLPLVPETETVHLVCGTHGKPELSGGAGVHFNLSHAGDYAAAVFD
                     EGEVGIDVEQVRRVNVNVMRRQFTAEENAYLLSEPDAFLRLWTRKESYCKAVGEGLHL
                     PLSSFDVLEDQVAGRTAPAAEAAVREADAGQFYLYTEVPVKGYVLSVCAGHPITGCPV
                     ENVDLRKLFDGIS"
     gene            complement(771..1124)
                     /locus_tag="ACZO9R_10425"
     CDS             complement(771..1124)
                     /locus_tag="ACZO9R_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_function: GO:0043424 - protein histidine kinase binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HIR0913510.1"
                     /translation="MEELFDSLKNWGCDVDGALARFIGDRELYRSCLHTAAGDQNFEL
                     LPEALRAGEMGRAFECAHTLKGMLANMGLIPLYDIVVRIVEPLRAGEPAELLPVCDEL
                     LAARDRLNTLLKESA"
     gene            complement(1108..>1638)
                     /locus_tag="ACZO9R_10430"
     CDS             complement(1108..>1638)
                     /locus_tag="ACZO9R_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR0913511.1"
                     /translation="VGTLTDITEQRMMDQELQKYRQVLLAAEQTERTMLVVDDQEVNR
                     GLLKCIFEEQYRILEAENGADAFEVLNRESGKVDIILLDLAMPVMDGMEFLKRKQQDA
                     VLAGIPVIIITADDTTGHQIQMLQLGAEDYIVKPFVPEIAMRRVENVLGAGRLLKRVS
                     VAGEKDKGETDNGGTV"
BASE COUNT          356 a          557 c          336 g          389 t
ORIGIN      
        1 gctctgtcag ttcatcgtac atgatctatc ccatcctttt tggctgatga atttattata
       61 gtgattttag gaaatgccgt caaataattt ccgcagatca acgttctcca caggacaccc
      121 cgtgatggga tgtcccgcgc agaccgacaa tacatagcct tttaccggca cttctgtata
      181 cagataaaac tgcccggcat ccgcttccct gaccgccgcc tctgctgcag gcgcagtcct
      241 tcctgccacc tgatcctcca gcacatcaaa cgaagaaagc ggcaggtgca gtccttcccc
      301 taccgctttg caatagcttt ccttgcgcgt ccagagtctt aaaaacgcat ctggttcact
      361 cagcagatac gcattctcct ctgccgtaaa ctgtcttcgc atgacattca cattcacacg
      421 ccgcacctgt tccacgtcga tcccgacttc accctcatca aataccgcgg ctgcataatc
      481 tcccgcatgg gacaggttaa agtgcacccc cgccccgccg gaaagctccg gctttccgtg
      541 ggtgccgcac accagatgca ccgtctctgt ctctggcacc agaggaagta gggaacagcc
      601 catgcttcgc agaccatact ccagcagcag acctgcggca aggctcctca tgcgatcctg
      661 ccgcttcccg cagcgtgcaa ttttctcctg tctctctgtc ggaacatacc gcaaaagttc
      721 ctcatacagc ccctcatcac acagagcgat gatatttgtc atcagaaccc tcatgccgac
      781 tcctttaaca gggtattcag cctgtctctc gccgccagaa gttcgtcgca gacaggtaaa
      841 agttccgccg gctctcccgc acgcagcggc tccacaatcc gcaccacaat atcgtatagc
      901 ggaatcagcc ccatattggc aagcattccc tttaatgtat gcgcacattc aaatgcacgc
      961 cccatctccc ctgcgcgcag cgcctccggc agaagttcaa aattctgatc ccccgccgcc
     1021 gtgtgcaggc agctgcggta cagctccctg tcgccgatga accgtgccag tgcgccatcc
     1081 acgtcacagc cccagttttt cagggaatca aacagttcct ccattgtccg tctctccttt
     1141 atccttctct ccggcaacag acacccgctt caacagtctg ccagctccca gaacattttc
     1201 cactctgcgc atcgcaatct ccggcacgaa aggcttcacg atgtagtcct ctgcgcccag
     1261 ctgcagcatc tggatctgat gtccggtcgt gtcgtccgct gtgatgataa tcaccgggat
     1321 tcccgcaagg acggcatcct gctgctttcg ctttaaaaat tccattccgt ccatcaccgg
     1381 catcgcaaga tccaacagga taatatccac cttgccggac tccctgttta agacctcaaa
     1441 ggcatccgct ccgttttccg cttcaaggat ccggtactgt tcctcaaaaa tgcacttaag
     1501 cagtccacgg tttacctcct ggtcgtccac aaccagcatc gtccgctctg tctgctccgc
     1561 cgccagaagt acctgacggt atttctgcag ttcctgatcc atcatccgct gttccgtaat
     1621 atcggtaagc gtaccgac
//
LOCUS       DBMDQJ010000574         1638 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24647_length_1638_cov_0.900455, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000574 DBMDQJ010000000
VERSION     DBMDQJ010000574.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24647_length_1638_cov_0.900455"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="ACZO9R_10435"
     CDS             <1..353
                     /locus_tag="ACZO9R_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015541775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH36 C-terminal domain-containing protein"
                     /protein_id="HIR0913512.1"
                     /translation="RKLIPEQTAMYHKYHELIREGEYYRILSSRENHRSDCWAVASED
                     KSEVLVTYVQVLAQANMPSRKVRLRGFDPAKKYRLEGTDEVYSGEMLMNAGFRMKDFW
                     GDFVSRLYHFVAVD"
     gene            366..1367
                     /locus_tag="ACZO9R_10440"
     CDS             366..1367
                     /locus_tag="ACZO9R_10440"
                     /inference="COORDINATES: protein motif:HMM:NF012742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR0913513.1"
                     /translation="MAKVRLADIAKVAGVSAVTVHNAITGQKGVSDEMRARILEIAKE
                     MGYRQPGSGARNTQGKSLRCIGVVIPERYLAEYTTFYWKMYQEMALAATEKSCMVTVE
                     ILKQEMEDEMIVPCIAKEQTVDALILMGDTSRDYIHYLKKNVDLPIVFLDFYDNELAK
                     DAVIADNFHGMYLMTEYLYEHGLTKLAFVGSIHFSSSIMDRYCGFSKAVLEHRDVTPA
                     EWILEDRDERGQIEIHLPEQMPEAFVCNCDLTAGLLMLELEKRGISVPEDVSVVGFDN
                     YLYPGFPDLKITSYEINTKAMVKVALEKALKQIRGAASGKNMEIVSGQLVPKESVKL"
BASE COUNT          476 a          344 c          451 g          367 t
ORIGIN      
        1 agagaaagct gattccggag cagaccgcaa tgtatcacaa atatcatgaa ctgatccgcg
       61 agggagagta ttaccgtatt ctttccagcc gtgaaaacca cagatccgac tgctgggcag
      121 tagcatcgga ggataaaagc gaagtgcttg tgacctacgt acaggtgctg gcacaggcaa
      181 atatgccgtc ccgcaaggtt cgcctgcgcg gctttgatcc ggcgaaaaaa tatcgtctgg
      241 agggaaccga tgaggtttac agcggcgaga tgctgatgaa tgccggtttc cggatgaaag
      301 atttctgggg tgatttcgtc agcagactgt atcactttgt tgcagtagac taaggagcaa
      361 cggatatggc aaaagtaaga ttggcagata ttgcaaaggt ggcgggcgtc agcgccgtca
      421 ccgtgcataa cgcgattaca ggacagaaag gggtcagcga tgagatgcgc gcccggattc
      481 tggaaattgc aaaggaaatg gggtaccgcc agccgggaag cggagcgaga aatacacagg
      541 gaaaaagcct gcgctgcatc ggagtggtga ttccggaacg gtatctggca gagtatacga
      601 cattttactg gaaaatgtat caggagatgg cactggcggc gacggaaaag agctgtatgg
      661 tgaccgtgga gatcctgaaa caggagatgg aagatgagat gatcgtgccg tgcatcgcca
      721 aggagcagac cgtcgatgcg ctgatcctga tgggagacac gagccgcgat tacatccact
      781 atctgaagaa aaacgtggat cttccgatcg tatttctgga tttctacgac aatgagctgg
      841 caaaagatgc cgtgatcgca gataactttc acggaatgta tctgatgacg gaatacctgt
      901 atgagcatgg ccttacaaag ctggcttttg tcggttcgat tcatttttcc agcagtatca
      961 tggatcggta ctgcggtttt tccaaggctg ttctggaaca ccgcgatgtg acaccggcgg
     1021 aatggattct ggaggaccgt gatgagcggg gccagatcga aattcatctc ccggaacaga
     1081 tgccggaagc gttcgtctgc aactgcgacc tgaccgccgg ccttctgatg ctcgagctgg
     1141 aaaaacgcgg catctccgtg ccggaggacg tttccgttgt cggcttcgac aattacctgt
     1201 atccgggttt cccggatctc aagatcactt cgtatgagat caatacgaag gcgatggtaa
     1261 aagtagcgct cgaaaaagcg ctgaagcaga tccgcggcgc ggcatccggc aaaaatatgg
     1321 aaattgtctc cggacagctg gtgccgaaag agagcgtaaa gctttgattt ttataactga
     1381 attataaatt aagaaacggg gcattttgaa atgccccgtt tttgtacata ttacacaaca
     1441 aagtcactta aaaattgaca gtatctaacg gaacttattt tttaagcaat ttccagttga
     1501 aaaattaacc atctatgtta aaattaagct aaaggactgg gcgtgagggt gataaaacac
     1561 ggcggttcgg agaaaaaaga attaaaaaac aagattctgt ccactaacag aaacttaaaa
     1621 aaacaaagta caactcat
//
LOCUS       DBMDQJ010000575         1620 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_24954_length_1620_cov_1.069783, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000575 DBMDQJ010000000
VERSION     DBMDQJ010000575.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24954_length_1620_cov_1.069783"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1411
                     /locus_tag="ACZO9R_10445"
     CDS             <1..1411
                     /locus_tag="ACZO9R_10445"
                     /inference="COORDINATES: protein motif:HMM:NF024085.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3794 and LysM peptidoglycan-binding
                     domain-containing protein"
                     /protein_id="HIR0913514.1"
                     /translation="QGEKVLIRGKLVFRVLYRRESGGLQAMGGEIPFEETVNVPELSE
                     RDYTQIVWNIEDLNITMIHSRKLSAKAVLTLTVRAESRVDAQAAADVETDDAGLQVLK
                     RSVPAATLAVRRKDIYRVREEVSVSANKPNIETILWQEMRLRDVSVRPLDGKLHLDGE
                     LSVFLIYSGEGEHTPVQWLEESIPFSGEVELSEAVEEMIPAVGVRILHAEADKKPDSD
                     GEMREVEVEAVLELDIRLYKEESVELLSDLYSTGCEVVSEQGEVCFDRLLTRNSCKMR
                     VGGSVSFQKPERILQICHSSGIVRLDESRPSENGLAIEGVLEVSLLYLTDDDAAPVGA
                     ISEVLPFSGTVEAEGIREDCVWQLNLGLEQLGAVMLGGGEAEVKAQLTVELLVFQPMQ
                     EEVVTSVQTQPFDLKRWEQQPGIVGYIVQPGDSLWKIAKKFHTTVDQVREMNEIDGEE
                     AGAGARLLLIKEVTAGNCS"
BASE COUNT          373 a          332 c          573 g          342 t
ORIGIN      
        1 tcagggggaa aaggtgctga tccggggaaa gcttgtgttc cgggtgctgt accgccggga
       61 gtcgggcggg ctgcaggcga tgggcgggga gatcccattt gaggaaacgg tcaatgtgcc
      121 ggagctttcg gagcgcgact atacgcagat cgtatggaat atcgaagatt tgaatattac
      181 catgattcat tcgcgcaagc tgagcgccaa agcggtgctg acgcttacgg tgcgggcgga
      241 aagccgcgtc gatgcgcagg ctgccgcgga cgtcgagacg gatgatgcgg ggctgcaagt
      301 cctgaagaga agcgttccgg cagcgacgct tgcagtgcga cgcaaagata tctaccgggt
      361 ccgggaggaa gtaagcgtat cggcaaataa gccgaatatt gagacgattc tctggcagga
      421 gatgcgtctt cgggatgttt cggtgcgccc gctggatgga aaactgcatc tggacggtga
      481 attgtcggtg ttcctgattt acagcggcga gggcgagcac acgccggttc agtggttgga
      541 ggagagtatt ccgttttctg gtgaagtgga actctcggag gcggtggagg agatgatccc
      601 ggcggtcggg gtccggattc ttcatgcaga ggcggacaaa aagccggaca gcgatgggga
      661 gatgcgcgaa gtggaagtgg aggcggtgct ggagctggac atccgccttt ataaagaaga
      721 atcagtggaa cttttaagcg atctctattc gaccggctgc gaggtcgtga gcgagcaggg
      781 ggaggtgtgc tttgaccggc ttttgacgcg caacagctgc aaaatgcggg tgggcggaag
      841 cgtttctttt cagaaaccgg agcgaattct gcagatttgc cacagcagtg ggattgtccg
      901 gctggatgag agccgcccgt cagaaaacgg tctggcgatt gagggagtgc tggaagtcag
      961 tctcctgtat ctgacggatg atgacgcggc gccggtcggg gcaatttctg aggttctgcc
     1021 attttctggc acggtcgagg cggaggggat ccgggaagac tgcgtctggc agttgaatct
     1081 ggggctggaa cagctcggcg cggtgatgct gggcggcggt gaggcggagg tcaaggcgca
     1141 gctcacggtg gaactgcttg tattccagcc gatgcaggaa gaggtggtta catccgtgca
     1201 gacacagccg ttcgatttaa agcgctggga gcagcagcca gggattgtgg gttatattgt
     1261 gcagccaggg gattctctgt ggaaaattgc aaaaaaattc cacacgacgg tggatcaggt
     1321 gcgggaaatg aatgaaatcg acggggagga agccggtgca ggggcgcggc tgcttctgat
     1381 caaagaggta acggcgggta actgttcata ggtaggaatt ttagagagaa aaaatcgttt
     1441 ctgttcacgg gtgggcactt ttgggaccga aagtgccgta agaaaacgtg gtggcagcag
     1501 gaatttgccg tattgaaatt cgaagccacg gcaaattccg ttactggaca cgtatgcgtg
     1561 tacagtaaca aaaaatccag ccggaatggt tgcatttggg ggaaaacttt atatagtatt
//
LOCUS       DBMDQJ010000576         1612 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25118_length_1612_cov_1.222369, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000576 DBMDQJ010000000
VERSION     DBMDQJ010000576.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1612)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25118_length_1612_cov_1.222369"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="ACZO9R_10450"
     CDS             <1..1008
                     /locus_tag="ACZO9R_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR0913515.1"
                     /translation="LVTYPGYDSNRLANNVDTEYFNSLQDDKSLPQYNYATQQETAPG
                     STFKMVTSTAGLAEGSITIGERIRDLGVYENVSNHPKCWIFSSNGGTHGEINVSEALR
                     DSCNYYFYEVGYRLATNNYSTAYNDSLGIEKLQKYASLFGLNERTGIEIEEAAPQIAN
                     EFPVMAAIGQSNNNYTTVQLARYVTAVANEGTVYQYTLLNRLEDSDGNVLQTFEPQVR
                     NQIDVLDTEEWNAIHSGMRMVVENTKEFDGFPIEAAGKTGTAQQNLKRPNHALFVGYA
                     PYDDPEISIATRIAYGYTSHNAAKLARNVFAYYFNVENEDELLNGQAANGNSSSNEIS
                     D"
     gene            1027..>1612
                     /locus_tag="ACZO9R_10455"
     CDS             1027..>1612
                     /locus_tag="ACZO9R_10455"
                     /inference="COORDINATES: protein motif:HMM:NF046784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HIR0913516.1"
                     /translation="MSQAVIIKSSKNGINLVLDASMPFPELLTEILHKFTEAERFFAN
                     ASFAISFEGRELSDEEKYQIVDAIMEQTRVKILCILDSDEIRDAVIEQKCREMQQTNE
                     EKEARRQSRGCFYRGSLLPGERLETEESIVIIGDVPQEAVVVSQSDIVVLGELSGSAF
                     AGMSGNTNATITALSFLPEQYNIAGVYGSPMEKAK"
BASE COUNT          452 a          403 c          425 g          332 t
ORIGIN      
        1 cttgtcacct acccgggata tgacagcaac cgtctggcaa acaacgtcga caccgaatac
       61 tttaacagtc tgcaggatga caaatcactc ccgcagtaca attacgcgac ccagcaggag
      121 accgcaccgg gatctacctt caagatggta acctccacgg caggtctggc ggagggcagc
      181 atcacaatcg gggagcgcat ccgtgatctc ggcgtctatg agaacgtaag caaccatcca
      241 aaatgctgga ttttttcatc caacggcgga acccacgggg agatcaatgt ctccgaagcg
      301 cttcgtgatt cctgtaacta ctatttctac gaggtaggat accggcttgc cacgaacaat
      361 tattccacgg catataacga ttcgctcggc attgaaaagc ttcagaaata cgcgagcctc
      421 ttcggtttaa acgaacgcac cggaatcgag atcgaggaag cggcgccgca gatcgccaat
      481 gaattcccgg tcatggccgc tatcggacag agtaataaca actacaccac ggtacagctt
      541 gccagatatg tgacagccgt cgccaatgag ggaaccgtct accagtatac actgttgaac
      601 cgcctggagg attccgacgg aaatgtgtta cagacatttg agccgcaggt gcgcaatcag
      661 atcgacgtgc ttgatacgga agagtggaat gccatccatt ccggtatgcg catggtggtg
      721 gagaatacaa aagagtttga cggttttccg atcgaggcag ccggaaaaac cggtacggca
      781 cagcagaatt taaagcggcc gaaccacgca ttatttgtgg gatatgcgcc ttacgacgat
      841 ccggaaatct ccatcgcgac gcgtatcgca tacggttata cctctcacaa tgcggcgaag
      901 ctcgcccgga atgtatttgc ctactacttt aatgtcgaga atgaagatga actgttaaac
      961 ggtcaggctg caaacggcaa ttcttcatcc aacgagatca gtgattaaga gagaaaggcg
     1021 cagacaatga gtcaggcagt cattatcaaa agcagtaaaa acggaatcaa tctggtgctg
     1081 gatgcatcga tgccgtttcc ggaactgctc acagaaattc ttcacaaatt tacggaggcg
     1141 gagcgctttt tcgcgaacgc atcgtttgcc atctcttttg agggcagaga attaagcgat
     1201 gaagaaaaat accagatcgt agacgccatc atggaacaga ccagagtcaa aatcctgtgt
     1261 attttagaca gtgatgagat ccgtgatgcg gtgatcgaac agaaatgccg cgagatgcag
     1321 cagacaaacg aagagaaaga ggcgagaaga cagagccgcg gctgctttta ccgtggctcc
     1381 cttctgccgg gagagcgcct tgagaccgag gagagcatcg tcatcatcgg cgatgtcccg
     1441 caggaagcgg tggtggtatc ccaatcggat atcgtggtgc tcggcgaact ctcgggaagc
     1501 gcctttgccg gaatgagcgg caataccaat gccacgatca cggcgctttc tttcctgccc
     1561 gagcagtata acattgccgg cgtttacggc agtccgatgg aaaaagcaaa aa
//
LOCUS       DBMDQJ010000577         1610 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25159_length_1610_cov_1.188204, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000577 DBMDQJ010000000
VERSION     DBMDQJ010000577.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25159_length_1610_cov_1.188204"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="xseA"
                     /locus_tag="ACZO9R_10460"
     CDS             complement(<1..1095)
                     /gene="xseA"
                     /locus_tag="ACZO9R_10460"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012743047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR0913517.1"
                     /translation="MRKNIYSVGQVNTYIQNMFAQDFMLHQISIKGEVSNCKYHSSGH
                     IYFTLKDQTGTIAAVMFSRYRRGLAFQMKEGDKVVVTGSVEVYERDGKYQLYAREIEL
                     EGAGNLYLKFEALKRELEEMGMFDAAYKKPIPRYATRIGIVTAPTGAAIQDIRNIAAR
                     RNPYVQLILYPALVQGDGAAASIVRGIHALDVLGVDVIIVGRGGGSIEDLWAFNEEEV
                     ARAIFACNTPVISAVGHQTDTTISDYVADLRAPTPSAAAELAVFDVQLVEKWLMDYGT
                     QMRRQLELKLNQMRTRLMHDEEKLRYLNPRAKLKENEKRLDELGAALERRMQATLEQK
                     RHTLALLSGRLDGMSPLKKLAQGYSYKKCQR"
     gene            complement(1092..1487)
                     /gene="nusB"
                     /locus_tag="ACZO9R_10465"
     CDS             complement(1092..1487)
                     /gene="nusB"
                     /locus_tag="ACZO9R_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR0913518.1"
                     /translation="MTRRELREQIFKLLFGVEFHDRQELPEQIAVYEETEEPWDAKDG
                     AYISGKCEDIVAHLAEIDEAIDAVAEGWKTGRMGKVDLTLIRLAVYEMKYDEDVPVGV
                     AINEAVELAKKYGTDESPAFINGVLAKLV"
BASE COUNT          334 a          513 c          351 g          412 t
ORIGIN      
        1 acgctggcac ttcttatagg aatacccctg cgccagcttc ttaagcggcg acatcccgtc
       61 cagtcttcct gaaagaagtg ccagcgtatg gcgcttctgt tcaagcgttg cctgcatccg
      121 gcgctctaag gcggcgccca gctcgtccaa acgcttttca ttctctttga gcttggcgcg
      181 cggattcaga tagcgcagct tctcctcatc atgcatgaga cgcgtgcgca tctggttcag
      241 ctttaattcc agctggcgtc tcatctgtgt tccgtaatcc atcagccact tttcgacgag
      301 ctgcacatca aacactgcaa gctccgccgc cgcggaaggt gtcggtgccc gcagatccgc
      361 cacataatcc gatatcgtcg tatccgtctg atgtccgacc gccgagatca ccggcgtgtt
      421 gcatgcaaaa atcgcccgcg caacctcttc ttcattgaat gcccacagat cctcgatcga
      481 accgccgccg cgtcccacaa tgatgacgtc cacacccagc acatccagcg catggatgcc
      541 gcgcacaatg cttgctgcgg cgccgtcccc ctgcacgagc gcgggatata agatcaactg
      601 cacataggga ttccttctgg ccgcaatgtt gcggatatcc tgtatggcgg ctcccgtagg
      661 cgctgtcaca atgccgatcc tcgtggcata tctgggtatc ggctttttgt atgccgcatc
      721 gaacattccc atctcctcga gttcgcgctt taacgcctca aacttcagat agagattccc
      781 ggcaccctca agctcaatct ctctggcata gagctggtac tttccgtccc gctcgtacac
      841 ctcgacggag ccggtcacaa caaccttgtc tccctccttc atctgaaagg caagtccgcg
      901 gcggtacctg gaaaacatga ccgcggcgat cgtccccgtc tgatccttga gggtaaaata
      961 aatatgaccg ctggagtgat acttacagtt cgacacttcc cccttgatgc tgatctggtg
     1021 caacataaaa tcctgtgcga acatgttctg gatgtatgta tttacctgac cgacagaata
     1081 gatatttttt ctcataccag ctttgcaagt acaccgttga taaatgccgg cgactcatcg
     1141 gtgccatact ttttggcaag ctccaccgcc tcgttgatgg caacgccgac cggcacatcc
     1201 tcatcatact tcatttcata gaccgccagg cgaatcagcg tgagatctac ctttcccatg
     1261 cgtccggtct tccagccttc tgccacagca tcgatcgcct cgtcaatctc tgcaagatgc
     1321 gccacgatat cctcgcattt gccggaaata tacgcgccgt cctttgcatc ccacggctcc
     1381 tccgtctcct catagacggc aatctgctcc ggcagttcct gcctgtcatg gaactccacg
     1441 ccaaacagca gcttaaatat ctgttctctc aactctcttc tggtcataaa cctttccttt
     1501 ctctcatgtt ccgtcttctt cgtcacattt ccctgtacag gaaaaaagga tgtcgcaggg
     1561 acatccttta tcttattcac cgtgattcga tgcgttactt gttgctgccc
//
LOCUS       DBMDQJ010000578         1606 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25246_length_1606_cov_1.254485, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000578 DBMDQJ010000000
VERSION     DBMDQJ010000578.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1606)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25246_length_1606_cov_1.254485"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="ACZO9R_10470"
     CDS             complement(<1..338)
                     /locus_tag="ACZO9R_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015560799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-ATPase domain-containing protein"
                     /protein_id="HIR0913519.1"
                     /translation="MQSQNELRNLLQRIDHKSYPAYKDTRGSYDFGNYVLSIDHVQGD
                     PFAAPSKVSVHVRGGAAKFPADLYRMKCRRLAVCDYLLRQFGLEQQTIRITVTAEADR
                     PPVIPGFQQFP"
     gene            complement(387..>1606)
                     /locus_tag="ACZO9R_10475"
     CDS             complement(387..>1606)
                     /locus_tag="ACZO9R_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HIR0913520.1"
                     /translation="EYTMSEIIASVYRRIRDSGQREGILFIDEINCVSETLAPTMLQF
                     LQCKTFGNQKIPEGWVIVAAGNPPEYNKSVRDFDMVTLDRVRYMTVEADYRVWKENAR
                     AQHISGAILSYLELRPGNFYRVEADVDGMQFVTARGWEDLSNLMAVYEKLGFLVDEQV
                     IREYIHHADVAEDVAAYLDLYRKYQDDYGIPQILDGCVRPEIYARIYAAAFDERLSVV
                     HLLLDGLAAFFGRAASERALTDAWYAFLKEYRSQVETAADPAECYRTLVAEREQEMLR
                     QIANDAYTREEAKREKLLLEKLKAAQQEVTKDSAQRCFEAAKAGFDRQREVLVQTEEA
                     AGAALEHAFDFMEQAFENGEEMVVFVTELTITSESARFLSEHTCERYLTYNKQLLIGT
                     RRAELLSELRRDN"
BASE COUNT          335 a          527 c          358 g          386 t
ORIGIN      
        1 ggaaactgct ggaaccctgg tataaccggc ggacgatcag cctcagctgt gactgtaatc
       61 cggatggttt gctgttccag cccgaactgg cgcagcagat agtcgcacac tgccagcctg
      121 cggcatttca tccggtagag atccgccggg aacttcgctg cccctcccct gacatggacg
      181 ctcaccttcg acggcgcggc aaacggatct ccctgcacat ggtcgatcga caacacataa
      241 ttgccgaaat cgtagcttcc tctcgtatcc ttgtaagccg gataactctt atgatcaatt
      301 ctctgtaaca gatttctaag ttcattctgt gactgcatct ttttattcct tcttatattc
      361 cttttatatt ctttcctgcg aaatcgtcag ttgtcccgac gcagctccga caacagctcc
      421 gctcgtctcg ttcctattaa aagctgtttg ttgtatgtca gatagcgctc gcaggtatgc
      481 tcggagagaa accttgcgga ctccgacgta atcgtgagct ccgtgacaaa caccaccatc
      541 tcctcaccgt tctcaaacgc ctgctccata aagtcaaacg cgtgctccaa cgccgcgccc
      601 gccgcttcct cggtctgcac cagtacctcc cgctgccggt caaatcccgc ttttgccgct
      661 tcaaaacagc gctgtgccga atcttttgtc acttcctgct gcgccgcttt taatttttcc
      721 agtaaaagct tctcccgctt tgcctcctcg cgggtgtaag cgtcatttgc aatctgacgc
      781 agcatctcct gctcccgctc tgcgacaagc gtccggtaac attccgccgg atctgcggct
      841 gtctccacct ggctgcggta ctcttttaaa aaggcatacc acgcatccgt caatgcccgc
      901 tcagacgctg ccctgccgaa aaacgccgcc agaccgtcca gcagcaggtg taccaccgag
      961 agccgctcat caaacgccgc cgcatagatg cgcgcgtaga tctccggtct gacgcagccg
     1021 tccagaatct gcggaatgcc gtagtcatcc tgatatttgc ggtagagatc cagatacgcc
     1081 gccacatcct ccgccacatc ggcgtgatgg atatattccc ggataacctg ctcgtccacc
     1141 agaaaaccga gcttctcata gaccgccatc agattggaga gatcctccca tcctcgcgcc
     1201 gtcacaaact gcatgccgtc cacatccgcc tccacgcggt aaaagttgcc ggggcgaagt
     1261 tccaggtagc ttaagatcgc tccgctgatg tgctgtgccc tcgcattctc cttccagacc
     1321 ctgtaatccg cctccacggt catatagcgc acacggtcga gggttaccat atcaaaatcg
     1381 cgcacggatt tgttgtactc cggcggattg cccgccgcca caatcaccca tccctctggt
     1441 atcttctggt tgccgaaggt cttgcactgt aaaaactgca gcatcgtcgg cgcaagcgtc
     1501 tcagacacac agttgatctc atcgataaac aggattccct cccgctgccc gctgtcacga
     1561 atcctgcggt atacactggc gatgatctca ctcatcgtgt actccg
//
LOCUS       DBMDQJ010000579         1601 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25345_length_1601_cov_1.923333, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000579 DBMDQJ010000000
VERSION     DBMDQJ010000579.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25345_length_1601_cov_1.923333"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(38..271)
                     /locus_tag="ACZO9R_10480"
     CDS             complement(38..271)
                     /locus_tag="ACZO9R_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913521.1"
                     /translation="MTKRPLCLGAAGVLSGILAAAYGWSVFARALLACGVLACGILGG
                     IFADGYRPGDGISTAERKRTLDDLKMPAAPLWA"
     gene            complement(284..1270)
                     /locus_tag="ACZO9R_10485"
     CDS             complement(284..1270)
                     /locus_tag="ACZO9R_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR0913522.1"
                     /translation="MSREAYRWRKKLICLGLACLMFLSGSLAACGRQTEQSAYHIEYL
                     NKDKNRLVEEPYEPSATDTDGMIKEFLAKLSSDSDNVDYRKPIPNDVEITNYSLDGVL
                     LTLHFDEDYSKMSAVDEVLCRAAVVRTMTQIDGVDCVAFYIGDAPLTDAKGNLVGTMN
                     QDSFIENPGEQINSIQNTTLTLYFSNLDGDGLVKEVREDVYYSSNISMEKLIMEQLLD
                     GPKTKDAKSAIPEGTKLVSVSVVDGVCYVSLDEAFKNQDYKVNEAIVIYSIVDSLSEL
                     STISKVQISVNGDTSGVYRDNFPLADMYDRNMDYVTGAESAFSETEEPTETD"
     gene            complement(1285..>1601)
                     /locus_tag="ACZO9R_10490"
     CDS             complement(1285..>1601)
                     /locus_tag="ACZO9R_10490"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015520268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR0913523.1"
                     /translation="NLIENAVKYNRPDGWVHVSLNADHQYFYLKVEDCGIGIPEESLE
                     HIYERFYRVDKSHSREIGGTGLGLAITRSAVLMHRGAIKVFSTVGEGTIFNVRIPLNY
                     IS"
BASE COUNT          382 a          472 c          344 g          403 t
ORIGIN      
        1 ccggtattgc tctaataaaa aatagtattt ccgctattta tgcccagagc ggagcggcgg
       61 gcatttttaa atcgtcaagc gttctctttc tttccgccgt ggaaatcccg tcgcccggac
      121 ggtacccgtc agcaaaaatg ccgccaagga ttccgcaggc caggacgccg caggctagca
      181 atgcccgcgc gaatacgctc cagccgtatg ccgccgcaag aatccccgac agaacgcccg
      241 ctgcgccaag acacaaaggc cgttttgtca cacacttccc cctttaatcc gtttcggtag
      301 gttcctccgt ttccgaaaag gcgctctctg cccccgtgac ataatccata ttgcggtcat
      361 acatatccgc gagcgggaaa ttgtcgcggt agactccgct cgtatcgccg ttgacggaaa
      421 tctgaacctt gctgatcgtg gaaagctccg acagggaatc cacgatcgaa tagataacga
      481 ttgcctcgtt tactttgtaa tcctgattct tgaacgcttc atccaggctg acgtagcaga
      541 cgccgtccac caccgacaca gagacaagct tggtcccctc cggaatcgca gactttgcat
      601 ccttggtctt cggaccgtct aaaagctgct ccataatcag tttttccatg gaaatattgc
      661 tgctgtagta gacatcctcg cgcacttcct tgaccagacc gtccccgtcc agattggaaa
      721 aatacagcgt gagtgttgta ttctgaatgg aattgatctg ctctcccgga ttttcgataa
      781 agctgtcctg attcatggta ccgacaagat tccccttcgc atccgtaagc ggtgcgtctc
      841 caatgtaaaa tgccacgcag tccaccccgt cgatctgtgt catcgtccgg acaaccgccg
      901 cccggcacag tacctcatcg acagcactca ttttgctgta atcctcatca aaatgcaggg
      961 ttaaaagcac accgtccagt gaataattcg tgatttccac atcatttgga atcggcttcc
     1021 ggtaatccac attgtccgaa tccgaactca gtttcgcaag gaactccttg atcattccgt
     1081 cggtatcggt tgccgaaggc tcataaggtt cttccaccag acggttcttg tccttattca
     1141 gatattcgat atgataggca ctctgctccg tctgcctgcc gcacgccgcg agcgagcctg
     1201 acaggaacat cagacatgcc agtcccagac atattaactt ttttctccat cgataagctt
     1261 ctcggctcat agactctccc ttatctaaga aatataattc agcggaattc tgacgttgaa
     1321 aatcgttccc tctcccacgg tactgaacac cttgatggct ccgcggtgca tcagaaccgc
     1381 gctcctcgta atggcaagtc caagtcccgt tccgccaatc tccctggaat gagacttgtc
     1441 cacacggtaa aaacgctcat aaatatgctc taacgactcc tccggaatac cgattccgca
     1501 atcctccact ttcagataaa aatactggtg atcggcattc aaagagacat gtacccatcc
     1561 gtccgggcgg ttatatttga ctgcattttc gatcaggttg g
//
LOCUS       DBMDQJ010000580         1600 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25365_length_1600_cov_2.033356, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000580 DBMDQJ010000000
VERSION     DBMDQJ010000580.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25365_length_1600_cov_2.033356"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            27..1172
                     /gene="fucO"
                     /locus_tag="ACZO9R_10495"
     CDS             27..1172
                     /gene="fucO"
                     /locus_tag="ACZO9R_10495"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HIR0913524.1"
                     /translation="MANRIMLNQTSYHGAGAIAEIVNEAKAHDYKKAFVCSDPDLIKF
                     QVSTKVTDLLDQAGLSYEIYSNIKANPTIENVQTGVAAFKKSGADYIIAIGGGSSMDT
                     AKAIGIIIANPEFEDVRSLEGVAPTRKPCVPIIAVPTTAGTAAEVTINYVITDVERKR
                     KFVCVDPHDMPIIAVVDPEMMSSMPKGLTASTGMDALTHAIEGYTTKAAWEMTDMFHL
                     KAIEIISKSLRGAVANTPEGREGMALGQYIAGMGFSNVGLGIAHSMAHTLGAVYDTPH
                     GVACAMMLPIVMEYNADCTGEKYREIARAMGVEGVDAMSQEEYRKAAVAAVRKLSEDV
                     GIPSKLEAIKESDLDFLAESAHADACAPGNPKDASVEDLKELFRKIM"
BASE COUNT          453 a          330 c          463 g          354 t
ORIGIN      
        1 ttatgtatgg gaaaggggaa caaaaaatgg caaacagaat tatgttgaat cagacatcct
       61 atcatggggc gggagcgatt gcggagatcg tgaatgaggc gaaagcacat gactacaaaa
      121 aagcatttgt ctgctcagac ccggatctga ttaaatttca ggtatccaca aaggtaacgg
      181 atctgctgga tcaggcagga ctttcctatg agatttattc caacatcaag gcaaatccta
      241 cgattgagaa cgtgcagacc ggtgtggctg cgttcaaaaa atccggagcg gactatatca
      301 ttgcaatcgg cggcggatct tccatggata cggcgaaagc gatcggcatt atcattgcaa
      361 atccggaatt tgaggatgtc agaagcctgg agggcgttgc accgaccagg aagccgtgcg
      421 tgccgatcat tgctgtaccg acaaccgcag gtaccgctgc agaggttacg atcaactatg
      481 ttatcacgga tgtggagaga aagagaaagt ttgtatgcgt agatccgcat gatatgccga
      541 tcattgcagt ggtagatccg gaaatgatgt cctccatgcc gaagggactg acggcatcga
      601 ccggaatgga tgcgctgaca catgccatcg agggctacac gacgaaagca gcctgggaga
      661 tgacggatat gttccattta aaggctatcg agattatttc aaaatctctg cgcggcgctg
      721 tggcaaacac accggaggga cgcgagggta tggcactcgg acaatatatc gcaggtatgg
      781 gattttccaa tgtcggtctt ggaattgccc actccatggc acatacctta ggcgcggttt
      841 atgatacgcc gcacggcgtg gcatgtgcga tgatgctccc gattgtgatg gagtataatg
      901 cagactgtac aggcgagaag taccgtgaga ttgcacgggc gatgggcgta gaaggcgtgg
      961 atgccatgtc tcaggaagag taccggaaag ccgctgttgc agcagtcaga aagctctccg
     1021 aggatgtggg aattccgtca aaactggaag ctatcaaaga gtcagatctt gatttccttg
     1081 cagagtccgc gcatgcggat gcctgcgcac cgggcaaccc gaaggatgca tcggtggagg
     1141 atttaaaaga gctgttccgc aagattatgt aatccatctt acatgaacgc aattaagaag
     1201 tcaggttagg acagatccct tctttgccgg attgccggcg gagaggggat ttttttgttt
     1261 cataggaaat accgtgatat attaatatgt gaaagcagaa ccttcctatg aaataaaaaa
     1321 cgctccgcga ggatgcgcag ctcgcggtaa agaaaatatc gctttgcgaa ccgctcgcgc
     1381 gcggagaatc tcgcaagcga gattcttttt acatatcgtt tgagtgcgcc ggtgcgtgtc
     1441 agaaacaggt gagaaaagtc gtggcgggaa gctgtcaggt atgatagtat aaaagagaaa
     1501 gaagaaaatg aggagtggcg gtaaaaatga aaatcagaca gttgtatgcg tatgaactcc
     1561 gaccggcggc agcgctggtg atggagacat acagcaggat
//
LOCUS       DBMDQJ010000581         1590 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25552_length_1590_cov_1.703828, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000581 DBMDQJ010000000
VERSION     DBMDQJ010000581.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1590)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25552_length_1590_cov_1.703828"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="ACZO9R_10500"
     CDS             <1..270
                     /locus_tag="ACZO9R_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR0913525.1"
                     /translation="YEQSVTYGYYMVFLQMLYITIFLSANLGVMNLLPLPALDGGRLL
                     FLIVEVIRGKRVDPDKEGMVHFIGMMLLFALMFFTFFNDIRKLFG"
     gene            283..1335
                     /gene="ispG"
                     /locus_tag="ACZO9R_10505"
                     /gene_synonym="gcpE"
     CDS             283..1335
                     /gene="ispG"
                     /locus_tag="ACZO9R_10505"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR0913526.1"
                     /translation="MTKVVKIGDRVIGGGNPILIQSMTNTKTEDVAATVAQIQALTAA
                     GCDIIRCAVPTMEAAEALTEIKKQVSIPVVADIHFDYRLALAAIEHGADKIRINPGNI
                     GSTERVRAVVDAAKERGIPIRVGVNSGSLGKDLVEKYHGVTAEGIVESALDKVHLIED
                     MGYDNLVISIKSSDVMMCVKAHELIATQTDHPLHVGITESGTIISGNIKSSIGLGLIL
                     HQGIGDTIRVSLTGDPLEEIKSAKLILRTLGLRKGGVEVVSCPTCGRTRIDLIGLANQ
                     VENMVADIPLDIKVAVMGCVVNGPGEAKEADIGIAGGIGEGLLIKNGEVYKKVPEGEL
                     LEALRYELLHWNEQQK"
     gene            1366..>1590
                     /locus_tag="ACZO9R_10510"
     CDS             1366..>1590
                     /locus_tag="ACZO9R_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III N-terminal
                     domain-containing protein"
                     /protein_id="HIR0913527.1"
                     /translation="MAKRFFDVFPSLQVEGDMKNLLTETEVTKVGMNHERDHLRVYLS
                     STRLIHKKDIRQLESAIAQQIFKGRMMQVKV"
BASE COUNT          411 a          338 c          485 g          356 t
ORIGIN      
        1 tatgagcaga gcgtgacgta cggctattat atggtatttt tgcagatgct ttatattacg
       61 atcttcttat cggcgaacct cggtgtgatg aatctgctgc cgcttccggc gctggacgga
      121 ggcagactat tgtttctgat tgtcgaggtg atccgcggaa agcgcgtcga tcccgacaag
      181 gaaggcatgg tgcattttat tggcatgatg ctgttgtttg cactcatgtt ctttacattt
      241 tttaacgata tccgcaagct gttcggatag aaaggcaaaa agatgacaaa agtagtgaaa
      301 attggcgacc gggtgatcgg cggaggcaat ccgatcctga tccagtccat gacaaataca
      361 aagacggagg acgtggctgc aactgtggcg cagattcagg cgcttaccgc tgccggatgt
      421 gatattatcc gctgcgccgt gccgactatg gaggcggcgg aggcattgac cgagatcaaa
      481 aagcaggtga gcattcccgt tgtggcagat attcattttg attaccgcct ggcgcttgcc
      541 gcgatcgagc acggtgcgga taagatccgc atcaatccgg gaaatatcgg aagtacagag
      601 cgtgtgcgcg cagtggtgga tgccgcaaaa gagcgcggta ttccaatccg tgtcggcgtc
      661 aacagtggat ctctgggaaa agatcttgtg gaaaaatatc acggtgtgac ggcggagggg
      721 attgtcgaga gcgccctcga taaagtacat ctgatcgagg atatggggta tgacaatctg
      781 gtcatcagca tcaaatcctc cgatgtgatg atgtgcgtga aggcgcacga gctgattgcg
      841 acacagacgg atcatccgct gcatgtcggt atcacggagt ccggcaccat catcagcggc
      901 aatatcaaat cttcgatcgg actgggactc attctgcatc agggaatcgg tgatacaatc
      961 cgcgtatcgc tcacgggaga cccgttggag gagatcaagt ccgcgaagct gattctgcgt
     1021 accttagggc tgcgcaaagg cggtgtggag gtcgtgtcct gtccgacctg cggcagaacc
     1081 agaatcgatc tgatcgggct tgccaaccag gtggaaaata tggtggcaga catcccgtta
     1141 gacatcaagg tggcggttat gggctgcgtt gtcaacggac cgggtgaggc aaaggaagcg
     1201 gatatcggta ttgccggagg aatcggagag ggactcttga tcaagaacgg ggaagtgtat
     1261 aaaaaggtgc cggagggcga actgcttgag gcattgcgct atgagctgct gcactggaat
     1321 gagcagcaga agtagaacag actgaggaag aagtgagaaa aaggcatggc aaaacggttt
     1381 tttgatgtat ttccatcact gcaggtagaa ggcgacatga aaaatctgct gacggagacg
     1441 gaagtaacga aggtgggcat gaatcatgag cgcgatcatc tgcgcgtcta tttaagcagc
     1501 acgcgtctga ttcacaaaaa agatatccgc cagttggaat ctgccattgc acaacagatt
     1561 tttaagggca ggatgatgca ggtcaaggtc
//
LOCUS       DBMDQJ010000582         1575 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25881_length_1575_cov_1.437585, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000582 DBMDQJ010000000
VERSION     DBMDQJ010000582.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25881_length_1575_cov_1.437585"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="ACZO9R_10515"
     CDS             <1..1254
                     /locus_tag="ACZO9R_10515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR0913528.1"
                     /translation="VQQRECNHTITNVLEDYDRMHTILMEENDALRRTVEQLQVEMSE
                     VHRKIGMSTLFDVYQKTPRIIQIVASLNFGDAVGNDVRAIARALNEAGYITGIFTLAI
                     HPKIKDEGVYLINMLPELNENDLIIYHYATADELADIIKEAPCKVVLRYHNVTPPAFF
                     RGYDEGAEKVTREGLDEIADLKDAIDYGIVVSDFNKKDLIDMGYQCPIAVAPILIPFK
                     DYEQEPDKDVVTRYSDGKTNIVFVGRIVPNKKFEDVIACFAAYKEKYDPTARLFLVGN
                     YQETDLYYQYLQDVIKKCGVEDVIFPGHIAFNAILAYYKIADLFLCMSEHEGFCVPLV
                     EAMFFETPIVAYASTAIPGTLGGSGVLVETKEPEAVAEKMHQVITDQAYREEIIEKQK
                     RRLEDFSYDRIKEQILKEITDIQEK"
     gene            1288..>1575
                     /locus_tag="ACZO9R_10520"
     CDS             1288..>1575
                     /locus_tag="ACZO9R_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913529.1"
                     /translation="MATALLSIGLLVLFSIIGIMTFSFFDGERKGDLLFLAPAVGLSI
                     TTVLAVDCVALAPVRIMKYVVLGVFIVMLSVRGRKMLATAKNELKYKRFAIY"
BASE COUNT          462 a          304 c          424 g          385 t
ORIGIN      
        1 gtccagcaga gggaatgcaa tcacaccatc acgaacgtgc tggaagatta tgaccgcatg
       61 catacgattc ttatggaaga aaacgacgca ctccgcagga ctgtagagca actgcaggtg
      121 gaaatgtcgg aagttcatag aaagataggt atgagcacgc tgtttgacgt gtatcagaaa
      181 accccaagaa ttattcagat tgtagcctcc ctgaactttg gagatgcggt aggaaatgat
      241 gtgcgggcga ttgccagagc tttaaatgag gccggctaca taaccggaat ttttacactg
      301 gcgattcatc caaagatcaa ggacgaaggc gtctatctga tcaatatgct gccggaatta
      361 aatgaaaatg atctgattat ctatcactat gcgacggcag atgaactcgc cgacatcatc
      421 aaagaggcgc cctgtaaagt ggtgctgcgg tatcacaacg taactccgcc ggcgttcttt
      481 cgcggatatg atgagggcgc ggagaaggtg acgagagagg gactggacga gatcgcggat
      541 ctgaaggatg cgatcgatta cggaatcgtt gtctcggatt tcaataaaaa ggatctgatc
      601 gacatggggt atcagtgccc gatcgcagtg gcaccgattc tgattccgtt caaagattat
      661 gagcaggagc cggataagga tgtggtaacc cgctattccg acgggaaaac gaatattgtg
      721 tttgtcggaa ggatcgtgcc gaacaagaag tttgaggacg tgattgcctg ctttgcggca
      781 tacaaggaga aatacgatcc gaccgcaaga ctttttctcg tcggcaatta tcaggagaca
      841 gatctctatt atcagtattt acaggatgtc ataaaaaaat gcggggtaga ggatgtgatc
      901 ttcccgggac acattgcatt taacgcaatt ctggcttact acaaaattgc agatctcttc
      961 ttatgtatga gtgagcatga gggattctgc gtaccgctgg tggaggcaat gttctttgag
     1021 acaccgatcg ttgcgtatgc ttccacggcc attcctggaa cactcggcgg cagtggagtg
     1081 ctggtggaga ccaaggagcc ggaagctgta gcggaaaaaa tgcatcaggt cattacggat
     1141 caggcatacc gggaagaaat cattgaaaag cagaagagaa gactggaaga tttttcatac
     1201 gacagaatca aggaacagat tttaaaggaa attacagata tacaggaaaa gtaaagcgat
     1261 acaaacgtaa cgttttaagg aggcacaatg gcaacagcat tattaagcat cgggctgcta
     1321 gttttattct caatcatcgg aattatgacg ttttcctttt ttgacgggga aagaaaaggg
     1381 gatttgctct ttcttgcacc ggcggtagga ctttcgatta cgacagttct ggctgtcgac
     1441 tgcgtggcgc ttgctccggt acggatcatg aaatatgtcg ttttgggcgt atttatcgtc
     1501 atgcttagtg taagaggcag aaaaatgctt gcaacggcaa aaaatgaact gaaatataag
     1561 aggtttgcaa tctat
//
LOCUS       DBMDQJ010000583         1571 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_25969_length_1571_cov_1.587075, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000583 DBMDQJ010000000
VERSION     DBMDQJ010000583.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25969_length_1571_cov_1.587075"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /locus_tag="ACZO9R_10525"
     CDS             complement(<1..752)
                     /locus_tag="ACZO9R_10525"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0913530.1"
                     /translation="MELCSIASGSSGNCICVGTDECHVLVDAGISGKRIESGLNSIDL
                     KTEEMQGILITHEHVDHIAGLGVLARRYGLPMYATPGTIDAILHTKSVGTIDPALFHE
                     IHAGEDFTIGDMTIEPIAISHDAAEPVAYKMKQPGKSMAVMTDLGIYDDHIVEKLKGL
                     DVLLLEANHDVHMLQVGSYPYPLKQRILGEKGHLSNELSGRLLGEVLHDHFGTVFLGH
                     LSKENNYAELAYETVRLEVAMGDNPYKGDDFP"
     gene            complement(770..>1571)
                     /locus_tag="ACZO9R_10530"
     CDS             complement(770..>1571)
                     /locus_tag="ACZO9R_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR0913531.1"
                     /translation="EAAVCTLEQLDEYSSSDMKVVVNGRIVRCPKCLEVNGSLELPSY
                     EIKEGDAVETRSFYTVEQLAAFMDVEVDMDHEILVNNRVATMESLVYENFSVDWVVLS
                     FGAAAVNPAELPVAEQPVTAADGGTEQTADETLAETVEEVDAAENEAQNGTDSEETEH
                     RMSQEPSSVTDATGAESAVEQPAEGEVQQASQKTDLGLVVFINQQPVRLTGKKSYIFV
                     DIFDFYDFDLTASAGRAIITKLNGQQAQYSAVLSEGDQIELRWQEINR"
BASE COUNT          353 a          505 c          312 g          401 t
ORIGIN      
        1 atcggaaaat catccccctt ataaggatta tctcccatcg ccacttccag ccgtaccgtc
       61 tcgtacgcaa gttccgcata gttattttcc ttggacagat gtcctaaaaa aaccgtgccg
      121 aaatgatcgt gcagcacctc cccgagcagc cgcccggaaa gctcgttgga caggtgtccc
      181 ttctctccga gaatacgctg ttttaacgga tacggataac ttccgacctg cagcatatgt
      241 acatcatgat tcgcctcgag aagaagcacg tcaagcccct ttaacttctc cacaatatgg
      301 tcgtcataaa ttcccagatc cgtcatgacc gccatggatt ttccgggctg cttcatctta
      361 tacgccaccg gctcagccgc gtcatgcgag atggcaatcg gctcgatcgt catatctcca
      421 atcgtaaaat cctcccccgc atgaatctca tgaaacaggg cgggatcgat cgttccgacg
      481 gatttggtgt gcagtatcgc atcgatcgtc cccggagtcg catacattgg cagaccgtaa
      541 cgtctcgcaa ggacgccaag tcccgcaata tgatccacat gctcatgtgt gatcagaatt
      601 ccctgcatct cttccgtttt cagatcaatc gaattcagac cggattcaat ccgctttccg
      661 ctgattccgg catccaccaa aacatggcat tcatctgttc ccacgcagat acagttgcca
      721 ctgctgccgc ttgctatact gcataattcc attttatttc ttccactctc tatctgttaa
      781 tttcctgcca gcgaagctca atctggtctc cctcggaaag cactgccgaa tactgtgcct
      841 gctgtccgtt caactttgtg atgatcgctc ttcccgcaga tgccgttaaa tcaaaatcgt
      901 aaaaatcaaa aatatctaca aagatgtagc tcttctttcc ggtcagacgt accggctgct
      961 gattgataaa cacaaccagt cccagatccg tcttctgcga agcctgctgc acttcccctt
     1021 cagccggctg ctccacagcg ctctctgctc ccgtggcatc tgtcacagat gaaggttcct
     1081 gtgacattct atgctccgtt tcctctgaat ctgtaccgtt ctgtgcctcg ttctctgcgg
     1141 catccacttc ctctaccgtc tcagccagag tctcgtccgc tgtctgctcc gtcccgccat
     1201 ccgccgccgt cacaggctgc tccgcaaccg gaagttctgc cgggttcacc gccgctgctc
     1261 cgaaagaaag caccacccag tccacggaaa agttctcgta caccagactc tccatggttg
     1321 ccacgcggtt attcacaaga atctcatgat ccatgtccac ttccacatcc ataaatgccg
     1381 ccagctgctc cacggtatag aagcttctcg tctcaaccgc atcgccctct ttgatctcat
     1441 aggacggcag ttccagactt ccgttgactt ccaggcactt cggacagcgc acaatcctgc
     1501 cgttgacgac caccttcata tccgaagagg aatactcatc cagctgctct aaggtacaaa
     1561 ccgccgcttc a
//
LOCUS       DBMDQJ010000584         1562 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26138_length_1562_cov_1.449692, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000584 DBMDQJ010000000
VERSION     DBMDQJ010000584.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26138_length_1562_cov_1.449692"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /gene="ftsH"
                     /locus_tag="ACZO9R_10535"
     CDS             <1..1387
                     /gene="ftsH"
                     /locus_tag="ACZO9R_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR0913532.1"
                     /translation="GKSRAKLMTDDPAKRVTFANVAGLKEEKEELEEIVDFLRAPKKY
                     TRLGARIPKGVLLVGPPGTGKTLLAKAIAGEAGVPFFSISGSDFVEMFVGVGASRVRD
                     LFEEAKKNAPCIVFIDEIDAVARRRGTGMGGGHDEREQTLNQMLVEMDGFGVNEGIIV
                     MAATNRVDILDPAIMRPGRFDRKVHVGRPDVGGREEILSVHAKNKPLGDDVDLKQIAQ
                     TTAGFTGADLENLLNEAAIIAAKEDRAYITQTDIKKAFVKVGIGAEKKSRVISEKEKR
                     ITAFHESGHAILFHLLPDVGPVYSVSIIPTGAGAAGYTMPLPERDDMFNTKGKMLQDI
                     IVSLGGRVAEELVFDDITTGASQDIKQATRMAKDMVTKYGMSENIGLICYDNDDDEVF
                     IGRDLAHTRGYGEGVATTIDLEVKRIIDECYEKAKQMIAEHRDVLDACANLLLEKEKI
                     SQQEFEALFEQ"
BASE COUNT          407 a          329 c          481 g          345 t
ORIGIN      
        1 cggcaagagc cgcgcaaagc tcatgacaga tgacccggca aagcgggtga catttgccaa
       61 tgtggcaggt ctgaaagagg aaaaggaaga gcttgaggag atcgtggatt tcctgcgtgc
      121 cccgaagaag tataccaggc ttggcgcgcg tattccaaag ggtgttcttc tcgtgggacc
      181 tccgggaacc ggtaagacac ttctggcaaa ggcgattgcc ggtgaggcag gcgtgccgtt
      241 ctttagtatt tccggttcgg attttgtgga gatgtttgtt ggcgtcggcg cttcgcgtgt
      301 ccgtgatctg tttgaggaag ccaagaaaaa tgcgccgtgt atcgtgttta tcgatgagat
      361 cgatgctgtc gcaagacggc gtggtaccgg tatgggcggc ggccacgatg agcgcgagca
      421 gacattgaac cagatgctcg ttgagatgga cggtttcggt gtcaatgagg gaattatcgt
      481 tatggcggcg acgaaccgtg tggatatttt ggatccggcg atcatgcgtc cgggacgttt
      541 cgaccggaag gtgcatgtgg gacgtccgga tgtcggcgga agagaggaga ttctttctgt
      601 tcatgcgaag aacaagccgc tcggggatga tgtcgactta aaacagatcg cacagacgac
      661 tgcgggcttt accggcgcgg atcttgagaa tcttctgaat gaggcggcta ttatcgcagc
      721 gaaggaagac cgtgcgtata ttacacagac ggacatcaag aaggcgtttg tgaaggtggg
      781 aatcggcgcg gagaaaaaga gccgcgtgat ctcagagaag gaaaagcgta tcaccgcatt
      841 ccacgaatcc ggtcatgcga ttctgttcca tctgcttccg gatgtcggtc cggtctattc
      901 cgtatcgatc attccgacgg gagcgggagc tgccggttac acgatgccgc tgccggagcg
      961 tgacgacatg ttcaacacga aaggcaagat gctgcaggat attatcgtat ctctgggagg
     1021 acgtgtggcg gaggaacttg tatttgatga catcacgaca ggggcatctc aggacatcaa
     1081 gcaggcgacc cggatggcaa aggatatggt cacaaaatac ggaatgtcag agaacattgg
     1141 tctgatctgc tacgacaatg acgacgatga ggtatttatc ggcagagacc tggcgcacac
     1201 gagaggctat ggagagggcg ttgcgaccac cattgacctg gaagtgaagc ggatcattga
     1261 cgaatgttat gaaaaagcga agcagatgat tgcggagcac cgcgacgtgc tggatgcctg
     1321 cgcgaatctt ctgctggaga aggaaaaaat cagccagcag gagtttgagg cattgtttga
     1381 gcagtaattg tgcataacgg caatgaaaaa agaaaaaccg ggcagaaggt actatacaaa
     1441 atgaataaaa atgtgtgcac aaaattgtga agtttgtgca cacttatctg gcggggcatg
     1501 ctattataat aaccgtaaac cccccccaat acattatata tagtttttgc tataccccat
     1561 ag
//
LOCUS       DBMDQJ010000585         1557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26257_length_1557_cov_1.330357, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000585 DBMDQJ010000000
VERSION     DBMDQJ010000585.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26257_length_1557_cov_1.330357"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="ACZO9R_10540"
     CDS             <1..304
                     /locus_tag="ACZO9R_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF7768 domain-containing protein"
                     /protein_id="HIR0913533.1"
                     /translation="SPYSGDTERNIKNAKRYSRFAVDKHYLPIAPHIYFTQFMDDDIP
                     EERDTAIFMNWVLMSKCVELWVFGENISVGMKAEIDRAKRKHMKIRYFTEELEEKL"
     gene            301..1104
                     /locus_tag="ACZO9R_10545"
     CDS             301..1104
                     /locus_tag="ACZO9R_10545"
                     /inference="COORDINATES: protein motif:HMM:NF020291.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HIR0913534.1"
                     /translation="MKFTLYTADCTGNAKNTNYPHQKVITSEADLKKAVAFDHVCALY
                     DNFSRSDTNFQLSDVVPMDCDNDHSDDTDEWITPEKLSEMLTDVAFAVTYSRHHMLAK
                     GSVSARPRFHVFFPTTPCKDATFHKAIKARIYKELPFFDGNALDASRFLFGSKSEVVW
                     HEGSLTIEDWLTLMKSNRSIPEGQRNSTLSRIAGRLVKRFGVTDEARQKFLDKAAECN
                     PPLDDTELESIWNSACKFGSKVTSQDGYVPPDQFGQNPLLPDDFSGTGS"
     gene            complement(1143..1535)
                     /locus_tag="ACZO9R_10550"
                     /pseudo
     CDS             complement(1143..1535)
                     /locus_tag="ACZO9R_10550"
                     /inference="COORDINATES: protein motif:HMM:NF020075.6"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
BASE COUNT          394 a          422 c          357 g          384 t
ORIGIN      
        1 ctctccttac tccggagata cggagaggaa tatcaagaac gccaagagat acagccgctt
       61 tgccgtagac aagcactatc tgccgattgc accgcacatc tattttacgc agttcatgga
      121 cgacgatatt ccagaggaac gggatacagc catttttatg aactgggtgc tgatgagcaa
      181 gtgcgtggag ctttgggtgt tcggtgagaa tatctccgta ggcatgaagg cggagattga
      241 ccgtgcgaag cgtaaacaca tgaaaatccg ttatttcacg gaagaattgg aggaaaagct
      301 atgaaattta ccctgtatac tgccgactgt accggcaatg cgaagaacac caactaccca
      361 caccagaaag tcattacctc tgaagctgac ctaaagaagg cggtcgcctt cgatcatgta
      421 tgcgcactgt atgataattt ttcccgcagt gacactaact tccagctctc ggatgttgtg
      481 ccgatggact gtgacaacga ccattccgac gatacggacg agtggatcac gcccgaaaag
      541 ctgtcggaga tgctaacaga tgtggcattt gcggtcacat acagtcgtca tcatatgctg
      601 gcgaaaggct cggtatccgc ccgccctcgt ttccatgtat ttttcccgac aacgccatgc
      661 aaggatgcaa catttcacaa agcaatcaaa gcccgtatct acaaggaact tcccttcttt
      721 gacggcaatg cgctggatgc ctcccgtttt ctgtttggct cgaaaagtga ggtggtttgg
      781 cacgaaggga gtctgactat cgaggactgg ctgacactga tgaaatcgaa ccgcagcatt
      841 ccagaagggc agcgcaacag caccctgtcc cgtattgcgg gcagactggt caagcgtttc
      901 ggtgtgacgg atgaagcccg tcagaaattt ctggacaaag cagccgagtg caatcctcct
      961 cttgatgata cggaattgga aagcatctgg aacagtgcct gcaagttcgg cagcaaggtt
     1021 acctcgcagg acggatatgt tccgcccgac cagttcggac aaaatcctct cctgccggat
     1081 gatttttccg gtaccggatc gtagccgccg cggcagaacg gttgacagcg caacagtcgc
     1141 cagattatca aactgcttcc gataatcaac ccacgttttt tcaacgcttc caatgcatag
     1201 gaagccggca tctcgctggg aaccacaaac cgaagatatt cctccaacgg tacttcattg
     1261 acaatgaccc acccttgcgg ctcctcatag taatcaagtt ctcccggata tccttcccct
     1321 gcatcttttt ccttatgata aatactgcca ctctgatcca gcagaagaac acggatcttt
     1381 gcattcctcc ctggagatgt ccatattttt tcctgtccaa cagcttcccc tgtctcttcg
     1441 ccgccttcct cttctgatat cagtaattct tctcctgtct ctccttttct atccggatct
     1501 ctcctaagtg ccaggatcat cagaacccct gtcatacaca gcagaatcaa tgtcttt
//
LOCUS       DBMDQJ010000586         1557 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26260_length_1557_cov_1.160714, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000586 DBMDQJ010000000
VERSION     DBMDQJ010000586.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26260_length_1557_cov_1.160714"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="ACZO9R_10555"
     CDS             <1..488
                     /locus_tag="ACZO9R_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HIR0913535.1"
                     /translation="RTFFNLFVVWSLTALWHGANYNLLIWGGILLCCLLVEKLFLLRL
                     LDKSRVIGHLYVLFVVPLSWVAFAVTDLSELGVYMTRLFPFAGHAAGVINHLDYLKYL
                     NDYAPLFLLGVLFATPLPETCYRLIERRWIGRVILLGIFGLCMYYLAVSANNPFLYFN
                     F"
     gene            500..1420
                     /locus_tag="ACZO9R_10560"
     CDS             500..1420
                     /locus_tag="ACZO9R_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HIR0913536.1"
                     /translation="MDLRKLLKCPVTVALLILWAALSVVGYLGKDTIYKNYTVDIRTT
                     PYFVLVFDGLHDGIFPWSHVGAPGGIGENGKLPSILLPGESVSDTELAGGTETVGETE
                     TEEAPHTREFCMVDDDWFDDAVFIGDSRTVGLRDYGGLNNATFYASTGLGIYDMWTEK
                     FCEVDGEKTTLEDALSRRQFGKIYFQIGINEMGRGTLDGFMEQYAQSVQKFRELQPDA
                     IIYVQGIMRVAKAKSDSDKIFNNQGINERNMRIAELADNQTIFYIDVNDVVCDADGNL
                     RADLTFDNLHLYGSKYGIWVDFLKTKGIAP"
     gene            complement(1395..>1557)
                     /locus_tag="ACZO9R_10565"
     CDS             complement(1395..>1557)
                     /locus_tag="ACZO9R_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR0913537.1"
                     /translation="SGSSSFDIPFNRQQLADYLSVDRSAMSNELCHLRDEGLISFQKN
                     HFTVQFPSS"
BASE COUNT          354 a          318 c          469 g          416 t
ORIGIN      
        1 cgcgcacctt tttcaatctg tttgtggtgt ggagccttac cgcgttgtgg cacggggcga
       61 attataactt actgatctgg ggcgggattc tgctttgctg cctgttggta gaaaagctct
      121 ttctgctgcg cctgctggat aaaagcaggg tgatcggtca tctttatgta ttgtttgttg
      181 ttccgctcag ctgggtggcg tttgcggtca ccgatctgtc ggagcttggt gtttatatga
      241 cgcggctgtt cccgtttgcg gggcatgcgg caggggtcat caatcatctg gattatttaa
      301 aatatctgaa tgactatgca ccgctgtttc ttctgggcgt gctctttgca acgccgcttc
      361 cggagacctg ctatcggctg atcgagcgca gatggatcgg cagggtgata ctgctcggca
      421 ttttcgggct ttgcatgtac tatctggcgg tgtcggcaaa caatccgttt ttgtacttta
      481 atttttaaat gtgtggtgga tggatttgag aaagcttttg aaatgtccgg taacggtcgc
      541 actgcttatc ttgtgggcgg cgctgtcggt tgtcggctat ctggggaaag atacaatata
      601 taagaattat acggttgata tacgaacgac accatacttc gtcctggtgt ttgacggact
      661 gcatgacgga atttttccgt ggagtcatgt gggtgcgccg ggcggcatag gagagaacgg
      721 aaagctgccg tccatcctgc tgccggggga gagtgtctct gatactgagc ttgccggcgg
      781 gacggagacg gtcggagaga cagagacgga agaggcgccg cacacgcggg aattctgtat
      841 ggtggatgac gactggttcg acgacgcggt ctttatcggg gattcgagaa ctgtggggct
      901 gcgcgattac ggcgggctga acaatgcgac cttttacgca tccacagggc ttggaatcta
      961 cgatatgtgg acggaaaagt tctgtgaggt ggacggggag aagaccacgc ttgaggatgc
     1021 gctttccaga cggcagtttg gcaaaattta tttccagatc ggcatcaatg agatgggaag
     1081 aggaaccctg gacggtttta tggagcagta cgcgcagtcg gttcagaaat ttagagaact
     1141 ccagcccgat gcgatcatct atgtgcaggg tatcatgcgg gttgcaaagg ccaagtccga
     1201 ctcggacaaa atttttaaca atcagggaat caatgaacgc aatatgagaa ttgcggaact
     1261 tgcggataat cagactattt tttacattga tgtgaatgat gtcgtatgtg atgcagacgg
     1321 aaacttaaga gcggatctca cgtttgacaa tctgcatctg tacggctcga aatacggaat
     1381 ctgggtggat ttcctcaaga cgaagggaat tgcaccgtga agtggttctt ctgaaaagaa
     1441 atgagaccct cgtctcttag atgacacagc tcgttcgaca tggcgctccg gtctacggag
     1501 agatagtcgg cgagctgttg tctgttaaag ggaatatcaa aagacgaact tccggat
//
LOCUS       DBMDQJ010000587         1556 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26290_length_1556_cov_1.030241, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000587 DBMDQJ010000000
VERSION     DBMDQJ010000587.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26290_length_1556_cov_1.030241"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(199..>1556)
                     /locus_tag="ACZO9R_10570"
     CDS             complement(199..>1556)
                     /locus_tag="ACZO9R_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913538.1"
                     /translation="YVIVNYLYVGCMFIVAKVIETISYGIENAWNPGKSCILSPLYYL
                     NNNLRSSLIYTENGNGITGIKITGMPLIGIYAAAAVVLVVVACQMYKRRQIETAGDWV
                     SIGIIKPVFRWGAAICGGLVVGVTVISMLTDLPQLRKYPWLLLCSVVMGFLFFFGAEM
                     LLEKNFRVFRRKRVLEWAATAVITVVFVSLFEMDAFGLERYVPEVADVETAFVYLDYP
                     VQVKGEELATLTELQRQIIANKDAYQEVERTGSGFYYTTFLYYMQDGSKVERRYALPL
                     PEADGGADSTPAEQILAWESEPENLEREIFGIGNSECRFTSGYMDLYTSTGLNNTHMF
                     SEKEIEQILKAIRADIEDGNLAKYYQYCEQPEDTYYSNIELEYYSTGRYVDNWMYYYD
                     YHNSLEKLEGKAVDGQEMTVGGNLYLTFGSDCVHIVETLEKLGITNDEWKLMTNQEMM
                     Q"
BASE COUNT          396 a          475 c          295 g          390 t
ORIGIN      
        1 acccgtcgga ttgtccaaac gtggaagaga cacggaatcc gatgcaaaca ttcagccgga
       61 agaaatgctc acatttttct caaacgaatt tccacctgca tttccacatg aaatccccaa
      121 gtatgtttct aaaccatatt acttatcaac acaaagcaca atatgcttcg tgcggcagtt
      181 cctcttcctg ccgtatctct actgcatcat ctcttggttc gtcatcagct tccactcgtc
      241 atttgtgatg ccaagtttct caagcgtctc tacgatatgc acgcagtccg atccgaacgt
      301 cagatacaga ttgccgccca ctgtcatctc ctgcccgtcc acggcttttc cttccagctt
      361 ctctaaactg ttatgatagt cataataata catccagttg tcaacatacc tgcctgtact
      421 ataatattcc agttcaatgt tgctgtagta cgtgtcttcc ggctgctcgc aatactgata
      481 atacttcgcc agatttccat cctcgatatc cgcccggatg gcctttaaga tctgctcaat
      541 ctccttctcg gaaaacatgt gcgtattgtt aagccccgta gaagtataca gatccatata
      601 tccagaggta aaccggcact ctgaatttcc gataccaaaa atctcacgct ccagattctc
      661 cggctcgctc tcccacgcaa ggatctgctc tgccggtgta ctgtcagcac ccccatccgc
      721 ctcaggcagc ggaagtgcat accgccgctc caccttggaa ccatcctgca tataatacag
      781 gaacgttgta taataaaatc ccgaaccggt acgttctacc tcctgatagg catccttatt
      841 tgcaataatc tggcgctgca gctccgtcag tgtcgcaagt tcttctcctt tcacctgcac
      901 cggataatcc agatagacga acgctgtctc cacatccgcc acctccggca catagcgctc
      961 cagaccgaat gcatccattt caaacaggga tacaaacacc actgtgatca cggcagtcgc
     1021 cgcccactcc agcacgcgtt ttctgcgaaa taccctgaaa ttcttttcca gcagcatctc
     1081 tgctccaaag aaaaagagga aacccatcac cacactgcag agcagcagcc acggatattt
     1141 gcgcagctgc ggcagatccg tgagcatgga tatgaccgtg acaccgacga ccagacctcc
     1201 gcagattgcc gctccccagc ggaataccgg cttgatgatc ccgatgctga cccagtcgcc
     1261 cgccgtctcg atctgccgcc tcttatacat ctggcaggcc accacgacaa gcacaactgc
     1321 tgccgccgca tagattccga tgagcggcat gcccgtgatc ttaatgccgg taattccatt
     1381 cccgttctcc gtgtagataa gactgctgcg aagattattg ttcagataat aaagcggcga
     1441 taaaatgcag ctttttcccg gattccatgc attctcaatc ccgtagctga tggtttcaat
     1501 cacctttgca acaataaaca tgcatcccac atacagatag ttgacaatca cataat
//
LOCUS       DBMDQJ010000588         1555 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26308_length_1555_cov_1.260660, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000588 DBMDQJ010000000
VERSION     DBMDQJ010000588.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26308_length_1555_cov_1.260660"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(168..515)
                     /locus_tag="ACZO9R_10575"
     CDS             complement(168..515)
                     /locus_tag="ACZO9R_10575"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR0913539.1"
                     /translation="MVIRRGDIFYADLRPVVGSEQGGVRPVLIIQNDVGNRHSPTVIC
                     AAITSQMNKAKLPTHVELDSQKYALVKDSVVLLEQLRTIDKTRLKGKVCHLDHQILVK
                     IDKALEISLELYT"
     gene            complement(605..>1555)
                     /locus_tag="ACZO9R_10580"
     CDS             complement(605..>1555)
                     /locus_tag="ACZO9R_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HIR0913540.1"
                     /translation="AGKVVVADIGITPDAFGERKPAVAALEDADLSLLAARESHSNKG
                     TFGRLLVIAGSQGMSGAAYLCAKAAYASGCGLVRIFTPEENRAILQTQLPEAVITAYS
                     SKKAEAPQLLEAMSWADTIVCGPGIGTPDTAAGMVRTVIKNAAVPVLLDADALNVIAK
                     DTNLLLLPHTELVVTPHLGEMSRLTGDSVAYLQNHLIEAADEFARQYNVICVLKDEHT
                     ATAIPYSQTWLNVSGNAGLATAGSGDVLSGIIGGLMAQGIRVEQAAPLGVYLHGKAGE
                     AAGARRSMRGMTASDIHDGMCEILAGLEQTQSQPGKDGIS"
BASE COUNT          323 a          492 c          350 g          390 t
ORIGIN      
        1 ggcgataaag ccaaaaacca caaaatctaa tatgacaaat cctgcaaaaa tgaggatgcc
       61 cacagtgggg ctgcaaccat catccatata gtatcctttc tttcgtgtct ccggtattac
      121 acactaaaac atcagaaatc ataacatttt cgcgcataat agtcaagtta tgtatagagt
      181 tccagactga tctccagcgc cttgtctatt ttcacaagga tctggtgatc cagatggcac
      241 actttacctt tcagtctcgt cttatcgatc gtccgcagtt gttccaacag caccacggaa
      301 tccttgacca gcgcatattt ctggctgtca agctccacat gtgttggaag ctttgcttta
      361 ttcatctgcg atgtgattgc cgcgcagatg accgtcggac tgtgtctgtt tccaacatcg
      421 ttctgtatga tgagcaccgg ccgcacaccg ccctgttccg agccgaccac cggtcgcaag
      481 tctgcataaa agatatcccc acgtcgaatt accatacttt ccactccatt tctctctgga
      541 tcttttacgg atagtattgc catttttcac gggtgtattc cattttgttt ttattttccc
      601 aacactatga tatgccatct tttcccggct gtgactgcgt ctgttccagt ccggccagga
      661 tctcgcacat tccgtcatga atgtccgatg ccgtcatgcc gcgcatggag cgccgcgcac
      721 ctgccgcttc gcccgccttt ccgtgcaggt agacgccgag cggcgccgcc tgctccacac
      781 ggattccctg cgccatcagt ccgccgatga taccgcttaa gacatccccg cttcccgccg
      841 ttgcaagccc ggcattgccg gatacattca gccatgtctg gctgtacgga atcgcggtag
      901 ccgtgtgttc gtccttcaag acgcagatca cattatactg tcttgcgaat tcatccgccg
      961 cctcaatcag atgattctgc agatacgcca ccgaatcccc cgtcagccgg ctcatctctc
     1021 caaggtgcgg cgtcaccacg agttccgtgt gcggcagaag cagcaggttt gtatcttttg
     1081 caatcacatt cagcgcgtcc gcatccaaaa gcaccggaac cgccgcattc ttgatcactg
     1141 tgcgaaccat ccccgcagcc gtatccggcg tgccgatccc cggtccgcac acgatcgtgt
     1201 ccgcccagct cattgcctcc aaaagctgcg gcgcctccgc ctttttggag gaatatgccg
     1261 tgattaccgc ctccggaagc tgcgtctgta aaatggcgcg gttttcctcc ggtgtaaaaa
     1321 tgcgcaccag cccgcagccg cttgcatatg cggctttcgc gcaaagatat gccgctccgg
     1381 acattccctg gcttccggca atcacgagaa gtctgccgaa cgttcccttg ttggagtggc
     1441 tctcccttgc ggcaagaagg gacagatccg cgtcttctaa cgctgccacc gccggtttgc
     1501 gctccccgaa tgcatccggc gtaatcccga tatccgccac aacgactttc ccggc
//
LOCUS       DBMDQJ010000589         1550 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26420_length_1550_cov_1.239476, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000589 DBMDQJ010000000
VERSION     DBMDQJ010000589.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26420_length_1550_cov_1.239476"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="ACZO9R_10585"
     CDS             complement(<1..296)
                     /locus_tag="ACZO9R_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR0913541.1"
                     /translation="MVTIKDIARESGYSVSTVSRVLNNRNDVSPDARKKIEEVVAKFN
                     FVPNNNAKHLKQNNSKAIGVLVKGISNMLFASVVEEIQRMIEKTEYTLVVSYLD"
     gene            complement(347..1219)
                     /locus_tag="ACZO9R_10590"
     CDS             complement(347..1219)
                     /locus_tag="ACZO9R_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR0913542.1"
                     /translation="MAKNKKADNQVSAVRCAKHGWILTVFFSILAVLWLYPIFLVAMN
                     SLKKKGFITRYPFALPDHRSFFGIENFIKGVQKTNYFAAMGNSVIITVGSVAVIILCT
                     SMCAWYITRVHNKVNSTIYMLCLFSMIVPFQMVMFSLSKLANMMHLSNPIGIILVYLG
                     FGAGLAVFMFCGFVKSIPLEIEEAAMIDGCTPLQTFFQVVFPIMKPTTITVAILQAMW
                     IWNDYLLPYLVLDLKKYKTIPIVIQYLKGGYGAVDWGTMMAMLVLAIVPIIIFYAACQ
                     KYIIEGVVAGAVKG"
     gene            complement(1219..1473)
                     /locus_tag="ACZO9R_10595"
                     /pseudo
     CDS             complement(1219..1473)
                     /locus_tag="ACZO9R_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
BASE COUNT          401 a          406 c          320 g          423 t
ORIGIN      
        1 tcgtccagat aagacacgac cagcgtatat tccgttttct cgatcatgcg ctggatttcc
       61 tccacgacac tggcaaaaag catatttgag atacctttga ccagtacgcc gattgccttg
      121 gaattgttct gttttaaatg cttcgcattg ttgttcggca caaaattaaa ctttgcgaca
      181 acttcctcga tcttttttct tgcatccggg ctgacatcat ttctattatt aagcactctg
      241 gaaaccgtgc tgacggaata gcctgactct ctggcaatat cttttattgt aaccataact
      301 tttcctgcct gtttttgtct tcttgatttc actggcaatt attatatcag cctttgacag
      361 ctcctgcaac aacaccttct ataatatact tctggcaggc tgcataaaaa ataatgatcg
      421 gaacaatggc aagcaccagc atcgccatca tggtacccca gtcaaccgct ccgtagccgc
      481 cctttaaata ctggatgaca atcgggatgg tcttgtattt tttcagatcc agcaccagat
      541 aaggcagcag atagtcgttc cagatccaca ttgcctgcag aattgccacc gtgatcgtag
      601 tcggcttcat aatcgggaat accacctgga aaaaggtctg gagcggcgta cacccgtcaa
      661 tcattgccgc ctcctcaatc tcaagcggaa tgctcttcac aaaaccgcag aacataaaca
      721 ccgccagtcc ggctccgaat ccgaggtata ccagaataat accgatggga ttgcttaaat
      781 gcatcatgtt cgccagcttt gacagggaga acataaccat ctggaacggc acgatcatgg
      841 aaaacaggca taacatgtag atcgtggagt tcactttgtt atgtactctc gtaatgtacc
      901 atgcgcacat ggaggtacag agaatgatta ccgccacaga acctaccgtg atgataacgg
      961 agttccccat cgccgcaaaa tagttcgtct tctgcacgcc cttgataaaa ttctcaattc
     1021 cgaagaagga acggtgatcc ggcagtgcaa acggatatct cgtgatgaat ccttttttct
     1081 ttaaggagtt catcgccacg aggaaaatcg ggtacagcca cagtaccgca aggattgaga
     1141 aaaatacggt caaaatccat ccgtgtttgg cacatcttac cgcgctcacc tgattgtctg
     1201 cttttttgtt ctttgccatt attgctgcac ctccttactt ctggtaagtc tgttctgtag
     1261 cattgcaatg actgccacaa tgataaagaa taccaccgct ttcgcctgac cggcgccctc
     1321 ccatccggtt ctggtgtaga atgtctcata aatattcagt gccagcatct gggaactgtt
     1381 cgacggctca cccgcagtca gcgccaggtt ctggtcaaac agcttaaagg agttcgtcag
     1441 tgtcaggaat gtacagatcg taatcgacgg catacccctc cgcttccttg tcaatccata
     1501 gaaattcccc gtcaggcagc tttgcgctcc actccgtagg ctgtccgttt
//
LOCUS       DBMDQJ010000590         1544 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26551_length_1544_cov_1.517672, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000590 DBMDQJ010000000
VERSION     DBMDQJ010000590.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1544)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26551_length_1544_cov_1.517672"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            267..812
                     /locus_tag="ACZO9R_10600"
     CDS             267..812
                     /locus_tag="ACZO9R_10600"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR0913543.1"
                     /translation="MDQQVKRVKRELIKHGAILDMYEDTMELPDGKQETWDFISHRKG
                     AAAVVPVRGDGKILMVKQYRNAIDRMTLEIPAGSRDSVTEDTRVCAARELEEETGYCS
                     DDLTRILSLKTTVAFCDEFIDVYLAKNLKPGHQHLDEGEFIDVAAYGVDELCDMIYAG
                     TIQDAKTVAGILAYKNLLNQD"
     gene            862..1413
                     /locus_tag="ACZO9R_10605"
     CDS             862..1413
                     /locus_tag="ACZO9R_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913544.1"
                     /translation="MKREGCIVMLAFLGGIVLTNLLGANRQIHGQLLNQTFLQQYAYQ
                     KVDGNQLFGHVVMVRMQMLVLIFLLGRAVDGKVFSVLAKSMLSAGFGFLLVVGIMEYG
                     GYGCLIILAGLLPQWIFYTAALLFYANSKKRQSRIVGWNSWQDRTRQLAEAGLVTGCV
                     LLGTFVESFVNPAVFAWVLKIFC"
BASE COUNT          419 a          278 c          457 g          390 t
ORIGIN      
        1 ccgtgccgcc ttcatgtgtg aaagcagaac cttcctatga aataaaaaac gctccgcgag
       61 gatgcgcagc tcgcggtaag gaaaatatcg ctttgcaaac cgctcgcgcg cggagaatct
      121 cgcaagcgag attctttttc ataggaaata ccgtgtgatg ttaaagcgtg gaagatgttt
      181 cctgtagggc aaaatgcttc gcggaggccg cattgcggcg aaggcgggga tgttatatca
      241 gatgcagaat agaaaggatg ggtaatatgg atcagcaggt aaagagagtc aagagagaac
      301 tgataaaaca tggtgcaatt ctggatatgt atgaggatac catggaactt ccggatggaa
      361 agcaggagac atgggacttt atctcacacc gcaagggagc ggcggccgtt gtgccggtgc
      421 gcggggatgg aaagatcctg atggtaaagc agtaccggaa tgcgatcgac cgcatgactc
      481 tggagattcc ggcaggttcg cgcgacagcg tgacggagga taccagggta tgtgcagcaa
      541 gggaactgga ggaggagacc ggctattgca gcgacgatct gaccagaatc ttatctctta
      601 agacgacagt cgcattctgt gacgagttta ttgatgtcta tctggcaaaa aatttaaagc
      661 ccggacatca gcatctcgat gagggggaat ttattgatgt tgccgcctac ggggtggatg
      721 aattgtgcga tatgatctat gcgggaacca tccaggatgc caaaacggtt gccggaattt
      781 tagcatataa gaatctgctg aatcaggact aggagatccg cataaatata atacagaaaa
      841 gatatgagcg gagtgcaatg tttgaagcgg gaaggatgta ttgtgatgct ggcatttctg
      901 ggcggaattg ttctgaccaa tctgcttggc gcaaatcggc agatacatgg acagctgctg
      961 aatcagacat ttctgcaaca gtatgcttat caaaaagtgg atgggaatca gctgtttggt
     1021 catgtggtta tggtgcgcat gcagatgctg gttctcatct ttttgcttgg aagagcggtt
     1081 gacggaaagg tgttttccgt gctggcaaag agcatgctct cagcaggatt cggattcctg
     1141 cttgttgtcg gaattatgga atacggagga tatggctgcc tgatcattct tgccggattg
     1201 ctgccgcagt ggatttttta tacggcggcg ctgcttttct atgcgaacag taagaaaaga
     1261 cagagcagaa tcgtgggatg gaacagctgg caggatcgga cgcggcagct ggcggaagcg
     1321 gggcttgtga caggctgtgt gttattggga acgtttgtgg agagctttgt caatccggca
     1381 gtattcgcct gggttttaaa aattttttgc taaaattttt aaaataatta caatatatgg
     1441 tacggagaaa ataagggaaa cgagatgttg tagtttcgct ctaaatgccc gaattataag
     1501 ggaaaaatgc gattgctttt cggtttggtt ctgattataa tgaa
//
LOCUS       DBMDQJ010000591         1542 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26615_length_1542_cov_1.124219, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000591 DBMDQJ010000000
VERSION     DBMDQJ010000591.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1542)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26615_length_1542_cov_1.124219"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..531)
                     /locus_tag="ACZO9R_10610"
     CDS             complement(<1..531)
                     /locus_tag="ACZO9R_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR0913545.1"
                     /translation="MKKALSVLLTAAMAASMMAGCGSSSDGAASGTTTDNGAAAGTES
                     AGAAAADGAAFKIGGIGPTTGAAAIYGTAVMNGAQIAVDEINAAGGINGYQIAYKFED
                     DQSDAEKSVNAYNTLKDWGMQMLIGTTTSTPCTAVVEESHNDNLFQLTPSATAVESIQ
                     YDNAFRMCFSDPNQGTA"
     gene            complement(721..>1542)
                     /locus_tag="ACZO9R_10615"
     CDS             complement(721..>1542)
                     /locus_tag="ACZO9R_10615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR0913546.1"
                     /translation="LQAAASSFEIGLKMTDGFYIIKGRNVRSGEGAREMLFDRAAKGK
                     KSKELKGELKDLKKSLLNAGGEKAYVNEALRDYQAALEQGDTLREQYEEARRHLAQSR
                     AAIERLLECMKESPVSEVKKSLDELMGDLDQVYHDCSIRDDDLDFQSTIQSLKQMAAE
                     YEEHAAKAQTEAGSKLAAIMLRSELENVAAVLKDATGWEEPDFFALAYYLGHENRGSL
                     REMENAQRNSYLVDYCGERFWNRFLELCRRAEAEQMVRFMTRKYIYNTEESPSNS"
BASE COUNT          332 a          472 c          288 g          450 t
ORIGIN      
        1 tgcagtaccc tggttcgggt ctgagaagca catacggaat gcattatcat actggatact
       61 ctctactgct gtagcagacg gtgtcaactg gaacagatta tcgttatgtg actcttcaac
      121 tactgctgta caaggtgtag atgttgtcgt accgatcagc atctgcatac cccagtcttt
      181 cagagtattg taagcattga cagatttttc agcatcggac tggtcatcct caaacttata
      241 tgcgatctga tatccgttga taccgcccgc tgcgttaatc tcatcaacag caatctgtgc
      301 tccgttcata actgctgtac cgtagatcgc tgctgctccg gtagtcggtc cgataccgcc
      361 aatcttaaat gccgcgccat ctgccgctgc ggctcctgcg ctctctgtac ctgccgctgc
      421 gccgttatct gtggtcgtac cggatgctgc tccgtcggaa gagcttccac agcctgccat
      481 catacttgca gccatcgctg ctgtcaacaa tacactcaat gctttcttca taatattgtc
      541 ctccctcaag tgcctttatc ttttctgaaa taatagcaaa attagagatg attcacaatg
      601 ttaaaattac accaaattct tgatttattt tatcgtatga ttatgcaatt tgtctttttc
      661 aataaccata ggttatattg cgtattccag gcggctatat tgtccgttct tctgcatttt
      721 ttaactgttt gacgggcttt cttccgtgtt gtaaatatac ttgcgcgtca taaaacgcac
      781 catctgctcc gcctctgccc ggcggcacag ttccagaaac cggttccaga aacgctcgcc
      841 gcagtaatcc accagatagc tgttgcgctg cgcattctcc atctcccgca aggatccccg
      901 gttctcatgt ccgagataat atgcaagcgc gaagaaatcc ggctcctccc agcctgtcgc
      961 atccttcaat accgccgcca cattctcaag ctcactccga agcatgatcg ctgccagctt
     1021 gctccccgcc tcggtctgcg ccttcgccgc gtgctcctca tactccgctg ccatctgttt
     1081 taagctctga atcgtggact gaaaatcgag atcgtcatcc cggatggaac aatcatgata
     1141 gacctggtcc agatctccca tcagctcgtc caggctcttt ttgacttcac ttaccggact
     1201 ttccttcatg cactccaaca gacgctcaat cgccgccctc gactgcgcga ggtgccttct
     1261 cgcctcctca tactgttccc gcagagtatc cccctgttcc agcgccgcct gataatccct
     1321 cagcgcctca ttcacatacg ccttctcgcc ccccgcattc agaagactct tttttaaatc
     1381 cttcaactcc ccttttagct cttttgactt ctttcctttc gcagctctgt caaacaacat
     1441 ttccctcgct ccttctcccg atctgacatt tctacccttt attatataaa acccatccgt
     1501 catcttcaag ccaatttcaa acgaagaggc tgccgcttga ag
//
LOCUS       DBMDQJ010000592         1537 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26719_length_1537_cov_1.106546, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000592 DBMDQJ010000000
VERSION     DBMDQJ010000592.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26719_length_1537_cov_1.106546"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="ACZO9R_10620"
     CDS             complement(<1..491)
                     /locus_tag="ACZO9R_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HIR0913547.1"
                     /translation="MKDKKQKNEQNAGAIAAKPKKKKKVLIVVLVIVAVLVVWIGISI
                     HNATKQVAMAVNTVEVEPVQKRDLSDTISVKGTVAGASSTNVTSKAASEITSMNVQVG
                     DIVKEGDVLCTLDSTSIEEKIADLEKSMSNANAVSSINTQQAADALQQAKDDQTTTLA
                     AAQ"
     gene            complement(657..1514)
                     /gene="nudC"
                     /locus_tag="ACZO9R_10625"
     CDS             complement(657..1514)
                     /gene="nudC"
                     /locus_tag="ACZO9R_10625"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HIR0913548.1"
                     /translation="MIQDILPLHLQNQYEKKQPDDTSYMMIYRENKILVREGETLAFL
                     TYRELREICETAGEMAPQSIYLFSVGEETYFLTELPEHLTPEGYCFEKMFAVRSGKPK
                     EHILAAATAWHLYEWYRDNRFCGRCGQKLMHNGTLRMLFCPACGNQVFPKIAPAVIVG
                     VTDGEYILMTTYANREYKRYALIAGFTEIGETAEETVMREVQEEVGLKVKNIHYYKSQ
                     PWGFDQNLLLGYFCELDGERDIVLDTGELATAEWVHYSDVPEDPEGLSLTREMMSVFQ
                     QKFLSEKMS"
BASE COUNT          324 a          462 c          305 g          446 t
ORIGIN      
        1 ttctgcgccg ccgcaagcgt cgttgtctgg tcatccttcg cctgctgcag ggcgtccgcc
       61 gcctgctgtg tattgatgct gctcaccgca ttcgcattcg acatggactt ctctaagtct
      121 gcgatcttct cctcgatcga cgtggagtcc agtgtgcata agacgtcacc ctctttcacg
      181 atatcgccga cctgcacatt catggaagtg atctcggacg ccgcctttga cgtcacattc
      241 gtgctgctgg ctccggcaac cgttccctta accgaaatcg tatcggacag atccctcttc
      301 tgtaccggct ccacttccac ggtattcacc gccatcgcca cctgcttggt cgcgttatgt
      361 atggatatac cgatccagac caccagaact gccacaataa ccagcacaac gatcagtacc
      421 ttctttttct tctttggctt tgctgctatc gcgcctgcat tctgctcgtt tttttgcttc
      481 ttgtctttca ttctcgtctc tcccttacct gtattttaca aatttatggt gattgtatca
      541 ttatcacccc tcaaaaaaaa tgggtttaag taaaacttaa gaattcttag gaaatcctac
      601 cattttttta aagtttcttt gtactattgt aataatacag tatatttatt tgactgtcaa
      661 gacattttct cagacaaaaa tttctgctgg aatacggaca tcatctctct ggtcagcgac
      721 agcccctccg gatcttccgg cacatccgaa taatgcaccc actccgctgt cgcaagctct
      781 cccgtgtcca gcacaatgtc ccgttctccg tccagctcac agaaatagcc gagaagaagg
      841 ttctggtcaa atccccacgg ctggctctta taatagtgga tattcttcac cttaagaccg
      901 acttcctcct gcacttcccg catgaccgtc tcctccgccg tctccccgat ctccgtaaat
      961 ccagcgatga gcgcgtatcg tttgtactcc ctgttggcgt aggttgtcat cagaatatac
     1021 tctccgtccg tcaccccgac aatcaccgcc ggtgcgatct ttgggaacac ctggttgccg
     1081 catgcgggac agaataacat ccgcagcgtt ccgttgtgca tgagtttctg tccgcatctt
     1141 ccgcagaacc ggttatcccg ataccactca tacagatgcc atgccgtggc agccgcaagg
     1201 atgtgctcct tcggctttcc cgaccgcacc gcaaacattt tttcaaaaca gtatccttcc
     1261 ggtgtgagat gctccggcag ttccgtcaga aaatacgtct cctccccaac ggaaaacaga
     1321 tagatgctct gcggcgccat ttctcccgcc gtctcacaga tctcccggag ttcccggtaa
     1381 gtaaggaagg caagcgtctc tccctccctt acaaggatct tgttctccct gtagatcatc
     1441 atatagctgg tatcgtccgg ctgttttttt tcgtactgat tctgcagatg cagtggtaaa
     1501 atatcctgta tcatttcgtc tctcctttat tacataa
//
LOCUS       DBMDQJ010000593         1525 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_26987_length_1525_cov_1.656601, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000593 DBMDQJ010000000
VERSION     DBMDQJ010000593.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26987_length_1525_cov_1.656601"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..1105)
                     /locus_tag="ACZO9R_10630"
     CDS             complement(<1..1105)
                     /locus_tag="ACZO9R_10630"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR0913549.1"
                     /translation="MSIYDTLNEQQKLGVITTEGPVLILAGAGSGKTRVLTHRTAYLI
                     EEKGVNPYNIMAITFTNKAAGEMRERIDALVGYGSESIWVSTFHSTCVRILRRHIDRI
                     GYDTNFTIYDSDDSKSVMKEICKRLNIDTKIYKERSLLAAISSAKDELVSPTQFALQA
                     AASSDFAKKKQAQVYQEYQAALQKNNALDFDDLIVKTVELLQTDPEVLAYYQERFRYI
                     MVDEYQDTNTAQFELIRLLAGKYKNLCVVGDDDQSIYKFRGANIYNILNFEKHFPDAV
                     TIKLEQNYRSTQNILNAANSVIANNVGRKAKTLWTANGEGEKIDFEQFDTAYEEADYV
                     ARDIASGVREGKYGYGDYAVLYRTNAQSRLFEER"
     assembly_gap    1183..1192
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1277..>1525
                     /locus_tag="ACZO9R_10635"
     CDS             1277..>1525
                     /locus_tag="ACZO9R_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015568767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HIR0913550.1"
                     /translation="MDNDTKKFLSDLLAELQRIDYVRPEDIPNIDLYMDQITTFMDSQ
                     LETSKRHADDKILTKTMINNYAKNNLLPPPEKKKYTKEH"
BASE COUNT          375 a          405 c          324 g          411 t
ORIGIN      
        1 aacgctcctc aaacagacgt gactgtgcat tcgtgcggta aagcaccgcg tagtcgccgt
       61 aaccgtactt tccttcccgg acgccggacg caatatccct ggcgacataa tccgcctcct
      121 cgtaggcggt gtcaaactgc tcaaagtcga tcttctctcc ctcgccattt gccgtccaga
      181 gcgtcttcgc ctttctgccg acattgttcg caatgacgct gttcgccgca tttaagatgt
      241 tctgcgtcga acggtaattc tgttccagtt taatcgtgac cgcatccgga aaatgctttt
      301 caaagtttaa aatattgtag atattcgctc cgcggaactt gtagatcgac tgatcgtcat
      361 caccgaccac gcacaggttt ttgtatttgc ccgcgagaag acggatcagt tcaaactgcg
      421 ccgtgttcgt atcctgatac tcgtccacca tgatatagcg gaaacgctcc tgatagtagg
      481 caagcacctc cggatccgtc tgcagcagct ccaccgtctt tacaatgagg tcgtcaaaat
      541 ccaatgcatt gttcttctgc agcgctgcct gatactcctg atagacctgc gcctgctttt
      601 tcttcgcaaa atcactcgat gccgccgcct gcagggcaaa ctgcgtcgga cttacaagct
      661 cgtcctttgc agaggagatc gccgccagaa ggctgcgctc cttgtagatc ttggtgtcaa
      721 tattcaggcg cttgcagatc tccttcatca cactcttgga atcatccgaa tcatagatcg
      781 taaaattcgt gtcgtatccg atgcggtcaa tgtgtctcct taaaatgcgc acgcaggtag
      841 agtgaaacgt tgacacccag atgctctccg agccgtatcc gaccagcgca tcaattcgct
      901 cgcgcatctc gcccgccgcc ttgttagtga acgtgatcgc cataatattg tacggattga
      961 cacccttttc ctcgatcaga tatgccgtcc ggtgtgtcag cactctcgtc ttgccggaac
     1021 ccgctcccgc gagaatcagc accggtcctt cggtggttat aacgccgagc ttctgctgct
     1081 cgtttaatgt atcataaata ctcatgcctg tttttccttt tccgtttata gtgatatgag
     1141 aaagtataac acatttctct ctgtttttcg ataccataca gannnnnnnn nngggacact
     1201 gtcatataca tttcccaaca tttgtctctt gtcatctggt tttgaaaact atataatgtg
     1261 ataagaggtg acgcatatgg acaacgatac aaaaaaattt ctgagtgatc tgttagcaga
     1321 attacagaga attgattatg tcagaccgga agatattccg aacattgatt tatatatgga
     1381 tcagattacc acatttatgg attcccagtt agagacatcc aagcggcatg cggatgacaa
     1441 gattctgaca aagacgatga tcaacaacta cgccaagaac aatcttcttc ctccgccgga
     1501 gaagaagaaa tacaccaaag agcac
//
LOCUS       DBMDQJ010000594         1524 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_27019_length_1524_cov_1.338721, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000594 DBMDQJ010000000
VERSION     DBMDQJ010000594.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27019_length_1524_cov_1.338721"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(107..1345)
                     /locus_tag="ACZO9R_10640"
     CDS             complement(107..1345)
                     /locus_tag="ACZO9R_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012955.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR0913551.1"
                     /translation="MNRIRSGAVALCAALFLLLSGCGAASDGQAVRQQAALSGGALQD
                     GQMSEQQMTEQQENGEASTAGTEGQREEQMPDGTLSVTFLDVGQGNAVLVEQGGAYML
                     IDGGNRDYSSFVVSYLKEQGVEELAYVIASHYDADHLNGVVGALHAFSCGQVLAPDYV
                     TDTRVYESFERVIKEQDIALAYPAVGDTYTLGDASFTVVCPKAYDPKEDNDNSVGIRL
                     VYGDTSFLICGDAGKAEEQAMLDSGVTLESDVYLASHHGSEGSSSEAFMRAVSPSAVV
                     ISAGAGNSYGHPTRTVLDRVKACGAALYRTDLQGTITTQDYRDGDEVAAGAADTTGTS
                     GAADTAGSAAQASSGGETAAGGTADVTGEYVLNTNTKKFHRSSCSSVAQMSPENKAAF
                     SGSREELVAAGYDPCKRCNP"
BASE COUNT          296 a          506 c          347 g          375 t
ORIGIN      
        1 aacaaaaatg gaatggccgc tctaccgcgc ttccattcca ttttttacgc tttctgttaa
       61 aaatcgttgc atttttctta tcatctgttg tgcatgcaga atcccgtcag ggattacatc
      121 tcttgcacgg atcatatccg gctgcaacca gttcctccct gctaccggag aatgccgcct
      181 tgttttccgg cgacatctgc gcaacactcg agcaggatga ccggtgaaac ttctttgtgt
      241 tcgtattgag cacgtattct cctgtcacgt ccgctgtgcc gcccgcggca gtctctcccc
      301 cggatgatgc ctgtgccgcg cttcctgcgg tatctgccgc cccggatgtt cccgtggtgt
      361 ccgcagcacc tgccgcgacc tcatccccgt cccggtaatc ctgcgtcgtg atggttccct
      421 gcagatccgt ccggtagagc gctgccccgc atgccttcac ccggtcgagt accgtgcgcg
      481 tcggatggcc gtaggagttc cccgcacccg cactgatcac taccgccgac ggcgacacgg
      541 cgcgcataaa agcttcggag gaagaaccct cactcccgtg atggctcgcc agatacacgt
      601 ccgactccag tgtcacgccg gagtcaagca tcgcctgctc ttcggctttt ccggcgtcac
      661 cgcagattaa aaagctggtg tcgccgtaca caaggcggat tccgacggaa ttatcgttat
      721 cctccttcgg atcataggct tttgggcaga ccaccgtaaa agaagcgtcg ccaagcgtgt
      781 aagtgtctcc cactgccggg taggcaagtg caatatcctg ctcctttatc acgcgctcaa
      841 acgactcata gactctggta tccgtcacgt aatcgggagc aagcacctgt ccgcaagaaa
      901 atgcgtgcag ggcgccgacc acaccgttta agtgatccgc atcatagtga gaagcgatca
      961 cataggcgag ttcttccacg ccctgttcct tcagatagct taccacaaac gaggaatagt
     1021 ctctgttgcc gccgtcgatc agcatatacg ctccgccctg ctccactaaa acggcattgc
     1081 cctgaccgac atccaaaaac gtcaccgaca gcgttccgtc cggcatctgc tcttctctct
     1141 gtccttccgt ccctgccgtc gatgcttctc cgttctcctg ctgctctgtc atctgctgct
     1201 ccgacatctg cccgtcctgc aaggctcccc cggacagtgc tgcctgctgc cttaccgcct
     1261 gcccgtccga tgctgctccg catcccgaca gcaaaagaaa cagggccgca caaagtgcca
     1321 ctgccccgct tctgattcta ttcacgttgt atttcttatt tttctgcttc cccgctgtct
     1381 gcatgtcctt tttcctcttc cataaaatac atgcgcgcag cgaaatcctg aaaataccgc
     1441 acctcactgt tgtatccggt tccgccgaac gcctgtttaa gcttcactcc atgatacatt
     1501 aacatatctc cggctgcatg gcaa
//
LOCUS       DBMDQJ010000595         1506 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_27405_length_1506_cov_1.689680, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000595 DBMDQJ010000000
VERSION     DBMDQJ010000595.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27405_length_1506_cov_1.689680"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="ACZO9R_10645"
     CDS             complement(<1..511)
                     /locus_tag="ACZO9R_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HIR0913552.1"
                     /translation="MAGYVYTNENCIGCNRCISVCPILTANQAVVVDGKPRIEVNHAQ
                     CINCGACFDACEHQAREYADDTEQFFADLQRGESISLLLAPAFLANYPREYEQILGGL
                     RQMGAKRIISVSFGADITTWAYINYITKNQFTGGISQPCPAVVNYIEMYAPELLPKLM
                     PVHSPMMCAA"
     gene            complement(575..>1506)
                     /locus_tag="ACZO9R_10650"
     CDS             complement(575..>1506)
                     /locus_tag="ACZO9R_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HIR0913553.1"
                     /translation="SQMLSGCHFFKVYVDGELEYVLLTKSSAEDAYMVGKLAVCQIRT
                     LVSAYMEQFDRNNFMQNILLGNMLVVDMYNKAQKLHIEQAERVVFVIELEGKKDGTAI
                     ELVKNLFAAKTRDFVTEVDEQSIVLIKDTREMGEDEDLASLASMIVDNMHAEAMVRVR
                     VGYGNRVNNLQDIAKSYQEAKMALEVGKIFYVEKETIAYRYLGIGRLIYQLPMSLCEM
                     FIHEVFGDEIPDVFNEETTTTIQKFFENNLNISETARQLYVHRNTLVYRLERLEKVIG
                     LDIRKFDDAMTFKIAMMVISHMQYVKNSDNALN"
BASE COUNT          366 a          440 c          302 g          398 t
ORIGIN      
        1 ttgccgcaca catcatcggg ctgtgtaccg gcatcagctt cggaagcagc tccggtgcat
       61 acatctcgat gtagttgacc accgcaggac acggctgcga gatcccgccc gtaaactgat
      121 tcttcgtaat ataattgata tacgcccagg tcgtaatgtc ggcgccaaag ctgacactga
      181 tgatccgctt tgcccccatc tgtcttaagc cgcctaaaat ctgctcatac tctctgggat
      241 aattcgccag aaatgccggc gccagaagca gtgaaatgga ttcgcctctc tgcagatccg
      301 caaaaaactg ctccgtatca tcggcatatt ccctcgcctg atgttcacag gcatcaaaac
      361 atgcaccgca gttgatgcac tgtgcatggt taacctcaat gcgcggcttc ccgtccacca
      421 cgacagcctg attcgctgtc agaatgggac agacggaaat gcaccggttg cacccaatac
      481 agttctcatt cgtgtaaaca tagccagcca taactcttcc actcccttat gctgtctttt
      541 gtttaatttt aacatatagt gttgttttca tcaactaatt cagtgcatta tcactatttt
      601 tgacatactg catgtgggaa atgaccatca ttgcaatctt gaatgtcatc gcatcatcaa
      661 acttgcggat atcaagcccg atcaccttct ccaggcgctc cagacggtac accaacgtgt
      721 tcctgtgcac ataaagctgt cttgccgtct cagagatatt cagattgttc tcaaagaact
      781 tctgaatcgt tgtcgtcgtc tcctcgttga acacatccgg aatttcatcc ccgaatacct
      841 catgaataaa catctcgcac agagacatcg gaagctgata aatcagtctg ccaataccca
      901 gataacggta tgcgattgtc tctttttcca catagaagat tttccccacc tcaagcgcca
      961 tctttgcctc ctggtaggat ttcgcaatat cctgcaggtt gttcacacgg ttgccgtagc
     1021 cgacccggac gcgtaccatc gcctccgcgt gcatattgtc cacgatcatt gacgccaggg
     1081 aggccagatc ctcgtcctct cccatctctc tggtatcttt gatgagcaca atgctttgct
     1141 cgtccacctc tgtgacgaaa tccctggtct tcgccgcgaa cagattcttg actagttcga
     1201 tcgccgtacc gtccttttta ccttccagct cgatcacaaa taccacgcgc tccgcctgct
     1261 cgatgtgaag cttctgcgcc ttgttataca tatcgaccac gagcatattg ccgagcagga
     1321 tattctgcat aaaattgttc cggtcaaact gctccatata agcagacaca agcgtccgaa
     1381 tctggcagac tgccagtttt ccgaccatgt acgcgtcctc cgcagaagat ttggtcaaaa
     1441 ggacatattc cagttccccg tccacataca ccttaaagaa atgacatccg gacagcatct
     1501 ggctct
//
LOCUS       DBMDQJ010000596         1505 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_27420_length_1505_cov_1.891738, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000596 DBMDQJ010000000
VERSION     DBMDQJ010000596.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27420_length_1505_cov_1.891738"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            69..1361
                     /locus_tag="ACZO9R_10655"
     CDS             69..1361
                     /locus_tag="ACZO9R_10655"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015521308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HIR0913554.1"
                     /translation="MLTSVTGINWGDEGKGRIVDLLTEKADYVVRYQGGNNAGHTVVT
                     DQGKFVLNLLPSGILHPTAVCVLGNGMVIDPDHLRHEIAMMQYMKVKVDASNLKISDK
                     ATICMPFHVRQDVLEEERLSKTGSAFGSTRRGIAYAYGDKYMKKTLRMGDLLELDAPS
                     VQHRLKVIIDSKNLVLENVYNQEPLSYDEIMEWCRSQAEFFKDYICDVGTLLGAAAGQ
                     GKNILFEAQLGALRDIDYGIYPFTSSSNTIASYAPIGAGIPGCALDNVVGVMKAYSTC
                     VGEGPFVAETAMGEAWNEKLRESGGEFGAATGRPRRVGPFDAVASRYGIRCQKAQDIA
                     LTKLDVLGAFDKIPVITGYRLHGVLLDSFPTDSRLEEAEPVIEELPGWECDISGCRSW
                     KDLPENARKYVCTLEKLVDHPIYLVSTGAERDAYLLRE"
BASE COUNT          401 a          312 c          451 g          341 t
ORIGIN      
        1 agaaaaagaa agacaagagt gtccaagaag ctggaggaca tccgcaatta gaaagagagg
       61 caattattat gttaacatca gtgacgggaa ttaactgggg cgatgaagga aagggaagaa
      121 tcgtggatct tctcacagaa aaggcggact atgttgtccg ttatcaggga ggcaataacg
      181 ccgggcatac ggtggtgacg gatcagggaa aatttgtgct gaatctgctt ccgtccggta
      241 tcctgcatcc gacggcagtc tgtgtgctgg gaaacggtat ggtgatcgat ccggatcatc
      301 tgcgccatga gattgcaatg atgcagtata tgaaagtaaa agtggatgcg tcgaacctta
      361 agatttcaga taaggcgacc atctgcatgc cgttccatgt gagacaggat gtgcttgagg
      421 aggagcggct ttccaagacg ggttccgcat tcggatcgac ccggagaggc atcgcctacg
      481 cctacggaga taaatatatg aaaaagacgc ttcggatggg cgatctgctg gaactggatg
      541 cgccgtccgt acagcatcgt ttaaaagtta tcatcgacag caagaatctg gtgctggaga
      601 atgtatacaa tcaggaaccg ctctcctatg atgagattat ggagtggtgc agaagccagg
      661 cagaattttt caaagattat atctgcgacg tgggaacatt gctcggtgct gctgccggtc
      721 aagggaaaaa tattctgttt gaggcgcagc ttggagcgct ccgcgatatt gattatggca
      781 tttatccgtt tacatccagc tccaatacaa ttgcaagcta tgccccgatc ggtgccggta
      841 ttccgggctg cgcgctcgac aacgtggtag gcgtcatgaa agcatattcc acatgcgtag
      901 gcgaggggcc gtttgtagcg gagacggcga tgggcgaagc ctggaatgaa aaattgcggg
      961 agagcggcgg agagttcggc gcagcaacag gcagaccgcg ccgtgtcgga ccgtttgacg
     1021 ctgttgcaag ccgttacggc atccggtgcc agaaggcgca ggatattgca ctgaccaaac
     1081 tggatgtgct cggagcattt gataaaattc cggtaattac gggataccgt ctgcacggtg
     1141 tgttgcttga ttctttcccg acggacagca gactcgagga ggcagaaccg gtgatcgagg
     1201 aactgccggg ctgggaatgc gatatttccg gctgcagaag ctggaaggat ctgccggaaa
     1261 atgccagaaa atatgtgtgt acactggaga aactcgtgga tcatccgatt tatctggtat
     1321 ctacgggagc agagagggat gcgtacctgc tgcgtgagtg acaagaacgc ctgcaagggc
     1381 attttcgata tccggttttg tttttatttc ataggaaata ccgtgataca ttcatgtgtg
     1441 aaagtggaac tttcctatga aataaaaaac gctccgcgag gatgcgcagc tcgcggtaag
     1501 gaaaa
//
LOCUS       DBMDQJ010000597         1505 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_27428_length_1505_cov_1.289174, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000597 DBMDQJ010000000
VERSION     DBMDQJ010000597.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27428_length_1505_cov_1.289174"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="ACZO9R_10660"
     CDS             <1..632
                     /locus_tag="ACZO9R_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005348645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HIR0913555.1"
                     /translation="GDRLSSTYFTENKRPFHNFGNSLVRKSINTLFKNNIKDIMTGYR
                     AFSYEFVKTFPVLSKGFEIETEMSIHAIDKNMFVENVVITYRDRPEGSVSKLDTYSDG
                     FKVLKTIARLYRAYKPMGFFGIIALILAVIATAFFIPVMGTYLATGLVPDFPTLIVCG
                     FTMLSAIQSLFVGVTLETMAQKNRQDFEMQLQRVTAEKKRLCGEKNDAD"
     gene            complement(633..>1505)
                     /locus_tag="ACZO9R_10665"
     CDS             complement(633..>1505)
                     /locus_tag="ACZO9R_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR0913556.1"
                     /translation="TREHFFYIQEAGYLKSLKPHMSRRSGLQSYLFLIVLSGSGEVAY
                     RDTAKPVPATGSDMGRRSGTSAAGDGGNGSLATVHAKAGDCFFLDCRNEYTHISSEDD
                     PWELMWIHFYGPQCDAYYDYFLKQQTWHFHTAHLAELTAAIQSVIRFHEEPTDDTDLL
                     TAQQIVNILTLICTETDRTGAEISALGSKLKQILHYLDEHYTETISLDFLAEEFYISK
                     YYLAREFKKEYGTTIIRYVLAKKITHAKELLRYSNSSIEEIARLCGMDDASYFNKVFK
                     KMEGCTASEYRKQW"
BASE COUNT          330 a          381 c          429 g          365 t
ORIGIN      
        1 tgggagaccg cctgtcctcc acatacttta cggagaacaa gagacctttt cacaatttcg
       61 gcaattctct ggtgcgaaag agcatcaata ccctgtttaa gaacaacatc aaggatatca
      121 tgacgggata ccgggcgttc agctatgagt tcgtcaagac gtttccggtg ctctcgaagg
      181 gctttgagat tgagacggag atgagcatcc acgcgatcga caagaatatg ttcgtggaaa
      241 atgtggtcat tacctaccgc gaccgcccgg agggaagcgt gtccaagctg gatacctatt
      301 cggatggatt taaggtgctt aagacgattg cgagactgta ccgcgcctat aagccgatgg
      361 gattcttcgg aatcattgca ctgatcctcg ccgtgatagc gaccgccttt ttcatcccgg
      421 tgatgggtac ttacctggca accggtcttg tgccggattt cccgaccctg atcgtctgcg
      481 gcttcacgat gctctctgcg atccagtcgc tctttgtggg cgtgacgctt gagaccatgg
      541 cgcagaagaa caggcaggac tttgagatgc agctgcagag agtgaccgcc gagaaaaagc
      601 gtctgtgcgg ggagaaaaat gacgcagact gatcaccact gcttccggta ttcggatgcc
      661 gtgcatcctt ccattttttt gaatacttta ttgaaataac tggcgtcatc catgccgcag
      721 agccgcgcga tttcctcaat cgaggaattg ctgtagcgca gaagctcttt ggcatgggtg
      781 attttttttg ccagcacata gcgtatgatc gtggtgccgt actctttttt aaattccctc
      841 gcaagataat acttgctgat gtagaactct tcggcgagaa aatcgaggga gatcgtctcc
      901 gtgtagtgct cgtccagata gtgcagaatc tgtttgagct tgctgcccaa tgcggagatc
      961 tccgcaccgg tgcggtcggt ctcggtgcag attaacgtga ggatattgac gatctgctgg
     1021 gcggtcagaa ggtcggtgtc gtcggtcggc tcctcgtgaa agcggatgac ggactggatt
     1081 gcggcggtca gctcggccag gtgcgccgtg tgaaaatgcc acgtctgctg tttgagaaaa
     1141 tagtcgtagt aggcatcgca ctgcggaccg taaaaatgaa tccacatcag ttcccaggga
     1201 tcgtcctcac tgctgatgtg ggtatattcg ttccggcagt ccagaaaaaa gcagtccccg
     1261 gccttggcgt gcacagtggc aaggctgccg tttccgccat cgccggcggc ggaggtgccg
     1321 ctccgtctgc ccatatcgct gccggtggca ggaactggct tggcggtgtc gcggtatgcg
     1381 acctcgccgc tgccggagag cacgatcaga aacaggtagg actgcagacc gctccgccgg
     1441 ctcatgtgcg gctttaaact ttttaaatac cctgcctcct ggatatagaa aaaatgctcg
     1501 cgcgt
//
LOCUS       DBMDQJ010000598         1504 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_27447_length_1504_cov_1.149679, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000598 DBMDQJ010000000
VERSION     DBMDQJ010000598.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27447_length_1504_cov_1.149679"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            78..>1504
                     /gene="cls"
                     /locus_tag="ACZO9R_10670"
     CDS             78..>1504
                     /gene="cls"
                     /locus_tag="ACZO9R_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HIR0913557.1"
                     /translation="MKKIAHLLMGRLTIVGVSILLQICWLFLVLWKFSYQFTYANLII
                     RAAAIVLVLVIANKWENPANKLSWTFLILLSPILGIMLYFLFGRSGLTKHTREQMDAV
                     NREVDACMPVDEEAVRLLQEADPSVLRQSKYIADWGHFPVYRNTATKYYESGEAMFPD
                     MLAALESAEHFIFLEYFIIEEGHMFHQIEDILEKKVKEGVEVRLIYDDVGCIGTLPPR
                     YYKQLQKKGIKCAAFNPFRPLLSVIMNNRDHRKIFVVDGAVGFTGGINLADEYINEVK
                     RFGYWKDTGVRIEGEAVWSLTSMFLSMWNYIVHSTEDYSRFMPAQHQKAPFENDGFVQ
                     PYGDSPLDHENVGENIYLNIINRAKNYVYIFTPYLIIDHEMLTALCNAAKDGVDVRIV
                     TPGVPDKKMIFLLTQSYYAPLIQSGVKIYQYTPGFIHAKCFVCDDEIATVGSVNLDFR
                     SLYLHFECGVFFYRSAVVAQVKEDCL"
BASE COUNT          364 a          329 c          439 g          372 t
ORIGIN      
        1 ggtgacgtga ccggcggttg acattttata tgaaaaaacg taagataaag gatacggttt
       61 tgaaagggag gagacttatg aagaagattg cacaccttct gatgggaaga ctgaccattg
      121 tcggggtttc gatattgctg cagatctgct ggctgtttct ggtgctgtgg aaattcagct
      181 atcagttcac ctatgcgaac ctgataatcc gcgctgcggc gattgttctg gtgcttgtga
      241 ttgcgaacaa gtgggagaat ccggcgaata aactttcctg gacgtttctg attctgttgt
      301 cgccgatcct tggaatcatg ctgtatttcc tgtttggacg ctccggtctg acgaaacaca
      361 ccagagaaca gatggatgcc gtgaaccggg aagtggatgc ctgcatgccg gtagacgagg
      421 aggcggtcag gctgctgcag gaggcggatc catcggtctt acgacagtca aaatatattg
      481 cggactgggg acattttccg gtctaccgga ataccgccac aaagtattat gagagcgggg
      541 aggcgatgtt cccggatatg cttgcagcgt tggagagcgc ggaacacttt atttttctgg
      601 agtatttcat tatcgaggag ggacatatgt tccaccagat cgaggatatc ctggagaaaa
      661 aggtcaaaga gggcgtggag gtacgcctga tctacgatga tgtcggctgc atcggcacac
      721 tgccgccgcg gtattataag cagcttcaga aaaagggcat caagtgcgct gcctttaatc
      781 cgttccggcc gttgctctcg gtaatcatga acaaccggga tcaccggaag atttttgtgg
      841 tggacggcgc ggtcggtttt acgggcggga tcaatctggc agatgaatat atcaatgagg
      901 taaaacggtt cggctactgg aaggacaccg gcgtgcgcat cgagggcgag gcggtctgga
      961 gtctgaccag catgtttttg tccatgtgga attacatcgt ccattccacg gaagattaca
     1021 gccggtttat gccggcacag catcagaaag caccctttga gaatgacggg ttcgtgcagc
     1081 cgtacggtga cagccctctg gatcatgaga atgtgggcga aaatatttac ctgaatatca
     1141 taaacagggc aaagaattac gtctatatct tcacaccgta tctgatcatt gaccatgaga
     1201 tgctgacggc gctctgcaac gcggcgaagg acggtgtgga cgtgcggatt gtgacgccgg
     1261 gggttccgga caagaagatg attttcctgc tcacgcagtc ctactatgca ccgctcatac
     1321 aaagcggggt aaaaatttat cagtacacgc cgggatttat tcacgcaaaa tgttttgtct
     1381 gtgacgatga gatcgcaacg gtgggcagcg tgaatcttga cttccgcagc ctgtatctgc
     1441 atttcgagtg cggcgttttc ttctaccgct ccgcggttgt ggcacaggta aaggaagact
     1501 gcct
//
LOCUS       DBMDQJ010000599         1501 bp    DNA     linear   ENV 29-APR-2026
DEFINITION  MAG TPA_asm: Roseburia hominis isolate MAG1363
            NODE_27522_length_1501_cov_1.427857, whole genome shotgun sequence.
ACCESSION   DBMDQJ010000599 DBMDQJ010000000
VERSION     DBMDQJ010000599.1
DBLINK      BioProject: PRJNA1288367
            BioSample: SAMN50508462
            Sequence Read Archive: ERR10114007
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseburia hominis (human gut metagenome)
  ORGANISM  Roseburia hominis
            Bacteria; Bacillati; Bacillota; Clostridia; Lachnospirales;
            Lachnospiraceae; Roseburia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Tang,Y., Kuang,J., Xia,X., Yao,C., Zhou,Z., Liu,J., Ren,Z.,
            Ding,K., Li,M., Li,Y., Jiao,F., Zheng,D., Chen,T., Zhao,A., Wan,X.,
            Ji,G., Zhang,S., Zheng,X. and Jia,W.
  TITLE     Targeting microbiota-generated acetaldehyde to prevent progression
            of metabolic dysfunction-associated steatotic liver disease
  JOURNAL   Cell Metab (2026) In press
   PUBMED   41775257
  REMARK    Publication Status: Available-Online prior to print
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Zhang,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2025) Department of Pharmacology and Pharmacy,
            The University of Hong Kong, 21 Sassoon Road, Pokfulam 2052, Hong
            Kong
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Date          :: 05-JUN-2025
            Assembly Method        :: SPAdes v. 4.2.0
            Genome Representation  :: Full
            Expected Final Version :: No
            Genome Coverage        :: 1.497x
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 02/14/2026 16:24:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.10
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,135
            CDSs (total)                      :: 2,113
            Genes (coding)                    :: 2,074
            CDSs (with protein)               :: 2,074
            Genes (RNA)                       :: 22
            tRNAs                             :: 19
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 39
            CDSs (without protein)            :: 39
            Pseudo Genes (ambiguous residues) :: 0 of 39
            Pseudo Genes (frameshifted)       :: 10 of 39
            Pseudo Genes (incomplete)         :: 27 of 39
            Pseudo Genes (internal stop)      :: 2 of 39
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Roseburia hominis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27522_length_1501_cov_1.427857"
                     /isolate="MAG1363"
                     /isolation_source="stool; NASH/Fibrosis stage >=2; MASH1"
                     /host="Homo sapiens"
                     /db_xref="taxon:301301"
                     /environmental_sample
                     /geo_loc_name="China: Hong Kong SAR"
                     /lat_lon="22.3193 N 114.1694 E"
                     /metagenome_source="human gut metagenome"
                     /note="metagenomic"
     gene            137..1429
                     /locus_tag="ACZO9R_10675"
     CDS             137..1429
                     /locus_tag="ACZO9R_10675"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014080393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR0913558.1"
                     /translation="MTYEEVVDQINQIRRFGNLAGVEVAGRMLKALGSPQAGIPLIHI
                     AGTNGKGSVSAFLCAILREAGLRVGMFTSPHLVDFRERIQINGEMISKEDTQRIGVQL
                     LAMDFGVQPTMFDVTLAMAILYFKEQRCDVMILETGLGGRLDATNAVGVPDVTVITKI
                     GYDHMAVLGNTLAEIAAEKTGIIKKGTSLVLESQEAEVMTVFEAKVREEGIIDFRLID
                     PLEIKEQTYRDGRQYFSFGAYRDLSMRMLGVHQYENAIAALLGAEAFFRRHTEWICGG
                     TKEREEGVRRAVCQGIAKTVWKGRMEILSKKPFLLVDGAHNSNGVEALRESLVALFPG
                     EKFHFIMGVMADKDYETMIGELLPLAIDFTTCTVECERALQADALADCIRRKGITAAS
                     ADDFASCFQRGRLGETHRTVAFGSLYFIGEIEALFEAK"
BASE COUNT          384 a          310 c          460 g          347 t
ORIGIN      
        1 ttgcaaattt caagaaaatc attatcataa atactgccgg tgtcagcagt atgtatgcaa
       61 atgatggcgg cgtaatcatg gcagtgtaag acacgcgggt gtgtcgctgc tgcccggaga
      121 ggaacgcggg acacagatga catatgaaga agtagtagat cagatcaatc agatcagaag
      181 attcgggaat cttgccggag tggaggttgc cgggcgcatg ttaaaggcgc tgggcagtcc
      241 gcaggcgggg attccgttga tacacatagc agggacgaac ggaaaaggtt ctgtctctgc
      301 ttttctctgc gcaattttaa gggaggcggg acttcgcgtc gggatgttta cctccccgca
      361 ccttgtggac tttcgggaac gcatccagat aaacggggaa atgatctcta aggaggatac
      421 gcagaggatc ggcgtgcagc ttcttgcgat ggattttggc gtgcagccga cgatgtttga
      481 cgttacactt gccatggcaa tcctctattt taaggaacaa cgatgcgatg tcatgatctt
      541 agagaccgga cttggcggga ggctggacgc aaccaatgcc gtaggtgtgc cggatgtaac
      601 cgtgatcaca aagatcggct atgaccacat ggcggtgctt ggaaatacgc ttgctgagat
      661 tgctgcggaa aagacgggca tcatcaaaaa aggcacctcg cttgtgcttg agagccagga
      721 ggcagaagtc atgacggtgt ttgaggcgaa ggtcagggag gaaggaatta tagatttccg
      781 gctgatcgac cctttggaga taaaagagca gacttaccgc gacggcagac agtatttttc
      841 gtttggtgcc taccgtgatc tgtccatgcg catgcttggc gttcatcagt atgaaaatgc
      901 catcgcggcg cttcttggtg cagaggcatt tttcaggaga catacagagt ggatctgcgg
      961 cgggacaaaa gagcgggaag agggcgtaag gcgcgcagtc tgtcagggga tcgctaagac
     1021 cgtatggaag ggcaggatgg aaatcctctc aaaaaaaccg ttcctgctgg tggacggcgc
     1081 acacaacagc aacggcgtgg aagcgttaag agagagcctc gtggcgcttt tcccgggaga
     1141 aaagtttcat tttatcatgg gcgtgatggc ggataaggat tatgagacaa tgatcgggga
     1201 attgctgccg cttgccattg attttacaac ttgcacggtg gagtgcgagc gggcgctgca
     1261 ggcggacgcc ctggcggatt gcatccggcg gaaaggaatt acagctgcat cagctgatga
     1321 ttttgcgtcc tgttttcaga gagggcggct cggtgaaaca catcgcacgg ttgcgtttgg
     1381 ttcgctctat ttcatcggtg agatcgaggc gctgtttgag gcaaaatagt tacgaaaaga
     1441 ttacagaata gagcgtttat gacaaaaatg atacaatttc acaaactttc agtaatagtt
     1501 c
//
